LOCUS       DAOQFI010000001         9556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_2307_length_9556_cov_3.445216, whole genome shotgun sequence.
ACCESSION   DAOQFI010000001 DAOQFI010000000
VERSION     DAOQFI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9556
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2307_length_9556_cov_3.445216"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /gene="prfB"
                     /locus_tag="PLE93_00005"
     CDS             complement(<1..1065)
                     /gene="prfB"
                     /locus_tag="PLE93_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157591830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNH85456.1"
                     /translation="MGARLAAIDEHVALLADYLDPEGLEAEVAEFEQVMQQPGFWDDQ
                     DKAASVSADHARVQRKLEGFRALSSEAGDLAEMAEMAELDEEIAAELEEQMSSVEARL
                     EELEEARLFSGEYDHGDAIVSVHAGAGGTDSQDWAEILLRMYLRWAERRGFSVEMREA
                     SEGEEAGLKSATFLLKGENAYGLIAAERGVHRLVRISPFDSSSRRHTSFAQVDVAPVV
                     DEDVEIEIDDAEIRVDTYRASGAGGQHVNKTDSAVRITHEPTGVVVQCQNERSQTQNK
                     AVAMQMLKSKLVELEERKRAEAVNQERGEVKDVAWGSQIRSYVLQPYTMVKDHRTDFE
                     VGDAQRVLDGDIDGFIREYLN"
     gene            1190..2275
                     /locus_tag="PLE93_00010"
     CDS             1190..2275
                     /locus_tag="PLE93_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNH85457.1"
                     /translation="MIIATAVALISTLGPAGSATASPDASASIINGNPASIGDWPWQV
                     AIASSTSKRPGQSPFYRTFCGGSIVAPTVILTASHCALDMYKGEVGDYTVISGRTNLN
                     NQAAGQEVFVTGIYFPRTSNGKILYKTRQAWDVSLLTLEEPLAGQPVKLLGPDEHSIL
                     KPGRRLIETGWGSTITGRDVYPKTLKMGGTNIQPPSVCHGLLGSRYFKPSLQVCLGDA
                     KANQANCYGDSGGPAVVATSAGYRQVGVTSFGTADNCAGLIPNVDVLVGGGPVRDWIQ
                     AGVQVLGGVDPVGSGGTAGAISGLCHMPGVRGLSIARARHLIKASGCRKVRVIHRGRG
                     NRVSRLPALKGWLWDASKPIRLLAGGR"
     gene            2297..3265
                     /locus_tag="PLE93_00015"
     CDS             2297..3265
                     /locus_tag="PLE93_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNH85458.1"
                     /translation="MSEASAAGGTDSGGEARRKRLIRVISVSITVAVLTIGMKLAAWL
                     LTGSVGLLSDAAESVVNLVAAGVALIVILWSTRPADEDHTYGHEKADYLSAGLEGALI
                     LLAAVTIAYAAIERLIHPVELNSVGIGLAISAAASVLNLLAARLLIRTGEEETSLVLV
                     ADGRHLMADVITSAGVIVGVALVWLTGLERLDPVIALVVAANIIRTGTGLVSDSMSGL
                     MDKALPEDELQKVSQVLDGFESDDIRFHALRTRRAGRRTFISIHVLVPGNWSVKQGHD
                     LLERVEEDLRACFEQTTVFTHLEPIEDPVSFADTGLDRTSNPHTGG"
     gene            complement(3295..3678)
                     /locus_tag="PLE93_00020"
     CDS             complement(3295..3678)
                     /locus_tag="PLE93_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH85459.1"
                     /translation="MRPVTPEEKTVREFVATFNRSELDEFVELLDPEVEIHGMRGLRK
                     GRDEARAWATKKPGGVQQTVLIENCRVAGDRVLLGVKREWRWEEDDELAYTDELAWLL
                     ELRGARVLSWKPFEDPEEARTAFEA"
     gene            complement(3739..6597)
                     /gene="secA"
                     /locus_tag="PLE93_00025"
     CDS             complement(3739..6597)
                     /gene="secA"
                     /locus_tag="PLE93_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNH85460.1"
                     /translation="MAIQIIDRALRMGEGRKFKAYEKRVESINRIEPEMKELEDSELL
                     EEANALRERAKNGESLDDLLPESFALTREAAFRAIGQRHYDVQLIGGMVVHDGAIAEM
                     KTGEGKTLTSTLAIVLNALGGKSVHVVTVNDYLSKRDALWMKPIYDMLGVSVGWIQEN
                     DDPAARREKYACDVTYGTNSEFGFDYLRDNMAASLEQKVQRTHDFAIVDEVDNILIDE
                     ARTPLIISGAPEEAAQTYYTFARLAKQLEGVPAKTKLKSMGESKDTSEADYDYEFDEK
                     HKTVSPTERGVDNAEKFVGVENLYMAENGNLVNHLVQSLKAESLYKKDKDYAVVDGEV
                     MIIDEFTGRILEGRRWSEGLHQAVEAKEGVAIREENQTLATITLQNYFRLYDKLSGMT
                     GTALTEAVEFMKIYKVPVVEVPTNRPISRDDQNDQIFKTKEGKWQAVIRELVARHETG
                     QPILVGTVSVEVSEMISAELKKQGIEHVVLNAKPEHAEKEGETIAQAGRKGAVTIATN
                     MAGRGVDIKLGGDPEGMAHPQVAKQGLNPEDEGYNEAVEKIAAELKPQCDAEAEEVKE
                     LGGLFICGTERHESRRIDNQLRGRAGRQGDPGASRFFLSAEDDVIRLFAGDRIYKILD
                     RLGPTDEEGHEMPLEAKMLTKTVENAQKKVEEQNFLIRKRVLEYDDVMNEQRRVIYKY
                     RGEILEGRDMSEIAREEIDGVIGRLVDEFMAGDIIEEWDLPGLDSQLHQIWPCAIDVA
                     GLPPESTEPEALKQDLCEDAQKAYDEREAEFGEEIMRYLERTLLMQVIDSRWREHLYD
                     MDYLREGIHLRGFAQIDPLVAYKNEGYTMFQELMNGIWEEFSRLIYNVEIEIEPDQNG
                     SFAPPAEEPDEQTYDYSGGTMEAQPSALAEVAAGATPGPADAGGVPGVVPGGVPNVGG
                     AVDPANSATVVKTEREDIGRNDPCWCGSGKKYKKCHGQ"
     gene            complement(6741..7109)
                     /gene="raiA"
                     /locus_tag="PLE93_00030"
     CDS             complement(6741..7109)
                     /gene="raiA"
                     /locus_tag="PLE93_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNH85461.1"
                     /translation="MRIEIRGRNVEVNDDMREQVTQRFERLGEQVSSLARLEVVFSEE
                     QNPAISDKFVAEATLHLKGVTLHAHEASPEMTHTIHELAEDMRRQVKRHREKRRKRRE
                     TRKLVNRLQGRETGGAQPGL"
     gene            complement(7189..7830)
                     /locus_tag="PLE93_00035"
     CDS             complement(7189..7830)
                     /locus_tag="PLE93_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85462.1"
                     /translation="MGLDRIAGMFVPSRCPLCRGAIDPDLLVCGECMRELNRSPVLRG
                     EPPEGVDRIVSCADHDGAPRDLLAAFKFRHLTTLGGLIAGYMADAAGQPGEQSLLVPV
                     PPASLRTWFRGFDPVAMLTARLAGLLDIPEPPVPVLARRGSGRQRGRGRAGRLSDPPD
                     IRPTEAAGRQVGGRTVLLVDDVMTTGATLSTAAGALLLAGAGSVSALTFTRRL"
     gene            complement(7883..8899)
                     /locus_tag="PLE93_00040"
     CDS             complement(7883..8899)
                     /locus_tag="PLE93_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNH85463.1"
                     /translation="MKAMVLAAGLGTRLRPVTRAVPKPMVPVLNRPVMEHIIRLLARH
                     GFTEVIANIHWYPDVIRDHFGDGSGFGIELSYSFEEELLGTAGGVRNASAFFDDDFLV
                     ISGDAMTDIDLSAMREFHRSHGGMATLATRRVDDTSEFGVVITDDEGRIQGFQEKPDP
                     DEALSDLANCGIYMFNREIFDYFPGPGESKLMAPGHPDDFADWAYDVFPAMLDAGIAF
                     YAHETDAYWNDIGTIEELVQSNFDGLFGDVDLGLPEKQPLPEVWVAGEVEGLDGAEID
                     GPVLIGDGARIGEGARLVGPLVLGEGCSIGAGAGVREAVLLSRSSLEKGAFLAGGLLG
                     PTRD"
     gene            complement(8899..>9556)
                     /locus_tag="PLE93_00045"
     CDS             complement(8899..>9556)
                     /locus_tag="PLE93_00045"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNH85464.1"
                     /translation="GTGGELGARARDAGIGVVGLPGFFQPRMTVGYMTVVSTYIAHLA
                     GVAPDVRRELEQAADFLDTVKGDMEHLARALAAQIGDTPVVVHGASLTTAPARRWANQ
                     FNENAKQLAFSAEIPEANHNLMEAWSKGSGGLGAVFLTDSGQSARERRRMKLTAESIE
                     GTGAQVFEVETLGETRSERLFWSVMFGDLVSVEVASLRGVDPLPVDEIQNFKRRLGNT
                     "
BASE COUNT         1529 a         3166 c         3033 g         1828 t
ORIGIN      
        1 gttgaggtat tcgcggatga agccgtcgat gtcgccgtcg aggacccgct gggcgtcgcc
       61 gacttcgaag tcggtgcggt gatccttgac catcgtgtag ggctggagca cgtaagagcg
      121 gatctgggag ccccaggcga catccttgac ctcaccgcgt tcctgattga ccgcctcggc
      181 ccgcttccgt tcctcgagct ccaccagttt cgacttgagc atctgcatcg cgaccgcctt
      241 gttctgggtc tgtgagcgct cgttctggca ctggaccact accccggtcg gctcatgggt
      301 gatccgcacc gccgagtcgg tcttgttgac gtgctggccg ccggcgccgg aggcccggta
      361 ggtgtccacc ctgatctcgg cgtcgtcgat ctcgatctcc acgtcctcgt cgacgaccgg
      421 ggcaacgtcc acctgggcga agcttgtgtg ccggcgggac gaggaatcga agggcgagat
      481 ccggaccagc cggtggacgc cccgttccgc ggcgatcaga ccgtaggcgt tttcgccctt
      541 gagcaggaag gtggccgatt tcagcccggc ttcttcgcct tcggatgctt cccgcatctc
      601 gaccgagaag ccgcgtcgct cggcccagcg caggtacatc cggagcagga tctcggccca
      661 gtcctgggag tcggtcccgc cggccccggc gtgaaccgag acgatcgcgt caccgtggtc
      721 gtattcgccg ctgaagagcc tcgcttcttc cagttcctcg agcctcgcct cgaccgagct
      781 catctgctct tcgagttcgg ccgcgatttc ctcgtcgagt tcggccattt ccgccatctc
      841 ggcgaggtcg cccgcttccg agctgagggc acggaaaccc tcaagcttcc gctggactct
      901 cgcgtggtcg gctgaaaccg acgcggcctt gtcctggtca tcccagaatc ccggctgctg
      961 cattacttgc tcgaactccg ccacttcggc ttcgagccct tcggggtcga ggtaatcggc
     1021 gaggagtgcg acgtgttcgt cgatcgccgc caggcgggct ccgatcgact cggtgttcag
     1081 ggcttcggcc acaaccggag cctacccatc ccgatgcctg cgaagatgta ccccaagcag
     1141 agtgtcaaca gggagaaacg tgacttcagc cagagggcgc atccgggtaa tgatcatcgc
     1201 caccgccgtg gccctgatct ccacgctcgg accggcagga tcagcgacgg cttcgccgga
     1261 cgcttcggcc agcatcatca acggcaatcc ggcttccatt ggcgactggc cgtggcaggt
     1321 cgcgatcgcc tcctcgacga gcaagcggcc gggccagtcc cccttctacc ggaccttttg
     1381 cggtggttcg atagtcgcac cgaccgtgat cctcacggcc agtcactgtg ccctggacat
     1441 gtacaagggc gaggttggcg actacacggt catctcgggc aggaccaacc tcaacaacca
     1501 ggcagccggg caggaagtct tcgtaaccgg gatctacttc ccccggacca gcaacggcaa
     1561 gatcctctac aagacccggc aggcgtggga cgtttccctg ctgacgctcg aggagccgct
     1621 tgccggccag ccggtcaagc tgctcggacc ggacgaacac tcgatcctca aaccgggacg
     1681 ccgcctgatc gaaaccggat ggggcagcac gatcaccggc agggacgtct acccgaagac
     1741 gctgaagatg ggtggcacga acatccagcc cccatcggtc tgccatggcc tgctcggttc
     1801 caggtatttc aagccctccc tccaggtctg tctcggtgat gcgaaggcaa accaggccaa
     1861 ttgctacggc gacagcggcg gaccggcggt cgtcgcaacc tcggccggct accggcaggt
     1921 cggggtaacc agtttcggca ccgcggacaa ctgcgccggc ctgatcccga atgttgacgt
     1981 gctggtcggc ggcggcccgg tccgcgattg gatccaggca ggagttcagg tactcggtgg
     2041 agtcgatccg gtcggcagcg gcggaacggc gggggcgatc agcggcctct gccacatgcc
     2101 gggagtcagg gggctttcaa tcgcccgggc ccgtcacctc atcaaggcca gcggctgcag
     2161 gaaggtccgc gtgatccacc gcggccgggg caacagggtt tcgaggctcc ccgccctcaa
     2221 aggctggctt tgggatgcca gcaagccgat taggcttctg gctggtggca ggtaacgaac
     2281 ctgaaagcag ttccacgtga gtgaggcatc cgccgcgggc gggacggaca gcggcggtga
     2341 agcccggcgg aaacggctga tccgggtcat ctcggtttcg atcaccgtcg cggtcctgac
     2401 catcggcatg aagctcgccg cctggcttct gaccggatcg gtcggcctgc tttccgacgc
     2461 cgccgagtct gtggtcaacc tggtcgccgc cggggtcgcc ctgatcgtga tcctctggtc
     2521 aacccgcccg gccgacgagg accacaccta cggccacgag aaggccgact acctgtcggc
     2581 cgggctcgaa ggagccctga ttctgctggc ggcggtgacc atcgcctacg ccgcgatcga
     2641 gcggctaata cacccggtcg agttgaactc ggtcggaatc ggcctggcga tcagcgccgc
     2701 cgcatcggtc ctcaacctcc tcgccgcgag gctgctgatc cggaccggtg aagaggaaac
     2761 ctccctggtc ctggtcgccg atggccgtca cctgatggcc gacgtgatca cctcggccgg
     2821 ggtgatcgtc ggggtcgcgc tggtctggct gaccggcctg gagcggctcg atccggtcat
     2881 cgcgctcgtc gtcgccgcca acatcatccg caccggaacg ggcctggttt ccgattcgat
     2941 gtccgggctg atggacaagg ccctgcctga agacgaactc cagaaggtca gccaggtgct
     3001 tgacgggttc gagagtgacg acatccggtt ccacgccctg agaacccgcc gggccggccg
     3061 acggactttc atttcgatcc acgtgctggt cccgggcaac tggagcgtga agcagggtca
     3121 cgacctgctc gaacgggtcg aagaggacct gcgggcctgt ttcgaacaga ccacggtctt
     3181 cacccacctt gaaccgatcg aggacccggt ctccttcgcc gacaccggcc tcgaccggac
     3241 ctccaacccc cataccgggg gctaacgagc cggcgagcct cgccccgccc gcggtcaggc
     3301 ttcgaaggcg gttcttgcct cttcggggtc ttcgaagggt ttccagctga ggaccctggc
     3361 gccccggagt tcgaggagcc aggcgagttc gtcggtgtag gcgagctcgt cgtcttcctc
     3421 ccagcgccac tcccgtttca cgcccagcag tacccggtcc ccggcaaccc ggcagttctc
     3481 gatcaggacc gtctgctgaa ctccgcccgg tttcttcgtt gcccaggccc gggcctcgtc
     3541 gcggcccttg cgcagacctc gcatgccgtg gatttcgacc tcggggtcga gcagctcgac
     3601 gaactcatcg agctccgacc ggttgaacgt cgccacgaac tcgcggactg tcttttcttc
     3661 aggagttaca ggcctcacca tccaggtcat acagcgagac catcagttgc ctctaccggg
     3721 cgaaatgacc tggatgcatc attgcccgtg gcacttcttg tacttcttgc ccgagccgca
     3781 ccagcaggga tcgttgcgcc cgatgtcttc gcgttcggtc ttgaccacgg tcgccgagtt
     3841 cgccggatcc accgcgccgc ccacgttcgg gacgccaccg ggaaccacac ccggaacccc
     3901 gccggcgtcg gccggaccgg gagtcgcgcc cgcagcgacc tcggccaggg ctgaaggctg
     3961 ggcttccatg gtgccgcccg agtagtcgta ggtctgctcg tccggctcct cggccggcgg
     4021 ggcgaacgat ccgttctgat ccggttcgat ttcgatctcg acgttgtaga tcaggcggct
     4081 gaactcctcc cagatgccgt tcatcagctc ctggaacatc gtgtagccct cgttcttgta
     4141 ggccaccagc gggtcgatct gggcgaaacc gcgcaagtga atgccttcac gcaggtagtc
     4201 catgtcgtag aggtgctcgc gccagcggga gtcgatcacc tgcatcaaca gggtccgctc
     4261 gaggtagcgc atgatctctt cgccgaactc ggcttcccgt tcgtcgtagg ccttctgggc
     4321 gtcttcacag aggtcctgct tgagcgcctc gggttcggtc gattccgggg gcaggccggc
     4381 cacgtcgatc gcgcaaggcc agatctggtg gagttgcgaa tcgaggcctg gcaggtccca
     4441 ttcctcgatg atgtcgccgg ccatgaactc gtcgaccagg cgtccgatca ccccgtcgat
     4501 ctcttcccgg gcgatctccg acatgtcgcg gccttcgaga atctcgccgc ggtacttgta
     4561 gatgacccgg cgctgctcgt tcatcacgtc gtcgtattcg aggacgcgct tgcggatcag
     4621 gaagttctgc tcctcgacct ttttctgggc gttctcgacg gtcttggtga gcatcttcgc
     4681 ctcgagcggc atctcgtggc cttcctcgtc ggtcgggccg agccggtcga ggatcttgta
     4741 gatgcggtcc ccggcgaaga ggcggatcac gtcatcctcg gccgagagga agaaccggga
     4801 cgcgccgggg tcaccctgac ggccggcacg gccgcgcagc tggttgtcga tccgcctgga
     4861 ctcgtggcgt tcggtgccgc agatgaacag gccgccgagt tccttgacct cttcggcctc
     4921 ggcgtcgcat tgcggtttca actcggcggc gatcttttcg accgcctcgt tgtagccctc
     4981 gtcttccgga ttgagtccct gcttggcgac ctgcggatgg gccatgccct ccgggtctcc
     5041 gccgagcttg atgtcaacgc cacggccggc catgttggtg gcgatcgtta ccgcgccctt
     5101 gcgtccggcc tgggcgattg tctcgccttc cttttcggcg tgctcgggct tggcgttcag
     5161 gaccacatgc tcgatcccct gcttcttcag ttcggccgag atcatttccg agacctcgac
     5221 tgaaaccgtg ccgaccagga tcggctggcc ggtctcgtgg cgggcgacca gttcgcggat
     5281 cacggcctgc cacttgcctt ccttggtctt gaagatctgg tcgttctggt cgtcgcggga
     5341 aatcgggcgg ttggtcggaa cctcgaccac cgggaccttg tagatcttca tgaactcgac
     5401 cgcttcggtc agcgcggtac cggtcatgcc cgagagcttg tcgtagaggc ggaagtagtt
     5461 ctgcagggtg atcgtggcga gggtctggtt ttcctcgcgg atcgccacgc cttcctttgc
     5521 ttcgaccgcc tggtggaggc cttccgacca gcggcggcct tcgaggattc ggccggtgaa
     5581 ctcgtcgatg atcatgactt caccgtcgac cacggcgtag tccttgtcct tcttgtagag
     5641 ggattcagcc ttcagcgact ggaccaggtg gttgaccagg ttgccgttct cggccatgta
     5701 gaggttctcg accccgacga acttctcggc gttgtccacg ccgcgctcgg tcggcgagac
     5761 ggtcttgtgc ttctcgtcga actcgtagtc gtaatcggct tccgaggtgt ccttgctttc
     5821 gcccatcgac ttcagcttgg tcttggcggg cacgccctcg agctgcttgg ccaggcgggc
     5881 gaaggtgtag taggtctggg ccgcctcttc cggggcaccg gagatgatca gcggggtgcg
     5941 ggcctcgtcg atcaggatgt tgtcgacctc gtcgacgatc gcaaagtcgt gggtgcgctg
     6001 gaccttctgc tcgagcgagg cagccatgtt gtcgcgcagg tagtcgaaac cgaactcgga
     6061 gttggttccg taggtgacgt cgcaggcgta cttctcgcgc cgcgcggcgg gatcgtcgtt
     6121 ctcctggatc cagccgaccg acacgccgag catgtcgtag atcggcttca tccagagcgc
     6181 gtcgcgtttg gagaggtagt cgttgacggt gaccacgtga accgacttgc cgccgagggc
     6241 gttgagcacg atggccaggg tgctggtcag ggtcttgccc tcaccggtct tcatttcggc
     6301 gatcgcgccg tcgtggacca ccatgccgcc gatcaactgg acgtcgtagt ggcgctggcc
     6361 gatcgcccgg aaggcagcct cgcgggtcag ggcaaacgac tccggaagca ggtcgtcgag
     6421 cgactctccg ttcttcgccc tttccctgag cgcgttggct tcttcaagca gctcgctgtc
     6481 ttcgagctcc ttcatttccg gctcgatccg gttgatcgac tcgacccgct tttcgtatgc
     6541 cttgaacttg cgcccctccc ccatcctgag cgcgcggtcg attatctgaa tggccatgcg
     6601 acaaggttag ccgctcggct tcaccgccga ctaaaggcgg agcccggctg caagacgaag
     6661 gggccggata tcggcccctc cgcactgact ggattgcttc cggtcccggg ccgttggccc
     6721 tgaccggcac ctttctcggc ctagaggccg ggctgcgcgc ctccggtctc acgtccctgg
     6781 aggcgattga caagctttcg ggtttccctc cgcttccttc tcttctcccg atgccgtttc
     6841 acctggcgcc gcatgtcctc ggcgagttcg tggatggtgt gggtcatctc cgggctcgcc
     6901 tcatgggcat gaagggtgac tcccttcagg tgaagcgttg cttcggccac gaacttgtcg
     6961 gaaatggccg ggttctgctc ctcggagaaa actacttcca ggcgggcgag cgacgaaacc
     7021 tgttcaccga gccgctcgaa ccgctgggtc acctgctcgc gcatgtcgtc gttgacttcg
     7081 acgttgcgac cacgaatctc gattcgcatg ggacctcctg gcgggttgtg ttgctttggc
     7141 cgatcgtagc cgggtaacag gatgatggca agggcaaagt ggcgaatttc acagccggcg
     7201 ggtgaaggtc agggccgaaa ccgacccggc accggccagc agcagcgccc ccgccgcggt
     7261 cgagagggtt gccccggttg tcatcacgtc atcgaccagc aggaccgtcc ggccgccgac
     7321 ctggcgaccg gcagcttcgg tcggcctgat gtcgggcggg tcggagagcc ggccggcccg
     7381 gccacggccg cgctggcgac cggaaccccg gcgggccagc accggaaccg ggggttcggg
     7441 aatgtcgagc aggccggcaa gccgggcggt cagcatcgcg accggatcga agccgcgaaa
     7501 ccaggtccgg aggctggccg gaggcacggg gacaaggagc gactgctcgc ccggctgccc
     7561 ggcggcatcg gccatgtacc cggcgatcaa cccgcccagg gtcgtgagat gcctgaactt
     7621 gaaggccgcc agcaggtccc tgggagcccc gtcatggtcg gcgcaggaga cgatccggtc
     7681 aaccccctcc ggtggctcac cccgcagcac cggtgagcgg ttcagctcgc gcatgcattc
     7741 gccgcagacc agcaaatcgg ggtcgatcgc gccccggcag agcgggcaac gcgaaggaac
     7801 gaacatcccg gcaatccggt caaggcccat cccccaacaa tccccgaaaa aacctcacaa
     7861 gtggctcaac aactgcaacg attcagtcac gagtgggacc caacaggccg ccggccagga
     7921 aggctccctt ttccagggat gagcggctga gcaggactgc ttcccgcacc ccggcccccg
     7981 ccccgatcga gcaaccctcg ccaaggacca gaggcccgac cagccgggcg ccctcgccga
     8041 tccgggcccc gtccccgatc aggaccggcc cgtcgatctc ggcgccatcg agtccctcga
     8101 cttcgcccgc gacccagacc tccggcaggg gctgcttttc gggcagcccc aggtcaacgt
     8161 cgccaaagag tccgtcgaag ttgctctgca cgagctcttc gatcgtgccg atgtcattcc
     8221 agtacgcgtc ggtctcgtgc gcgtagaagg cgatcccggc gtccagcatc gccgggaaga
     8281 cgtcataggc ccagtcggca aaatcgtcgg gatggcccgg ggccatcagc ttgctttcac
     8341 cgggaccggg aaagtagtcg aagatctcgc ggttgaacat gtagatgccg cagttcgcga
     8401 ggtcggagag agcctcgtcg gggtccggct tctcctggaa gccctggatt cgcccttcat
     8461 cgtcggtgat cacgacgcca aactcggaag tgtcatcgac ccgtcgggtc gcgagagtcg
     8521 ccatgccgcc gtgtgaccgg tggaactcac gcatcgccga aaggtcgatg tcggtcatcg
     8581 catcaccgga gatgaccagg aagtcatcgt cgaagaaggc cgaggcgttg cgtacgccgc
     8641 cggcggtgcc gagcagttct tcctcgaagc tgtaactgag ttcgatgccg aaacccgagc
     8701 cgtcgccgaa gtggtcccgg atcacgtccg ggtaccagtg gatgttggcg atgacttcgg
     8761 tgaagccgtg acgggcgagg agccggatga tgtgctccat gaccggccgg ttcaggaccg
     8821 ggaccatcgg cttgggcact gcccgggtga ctggcctgag cctcgtgccc agtccggcgg
     8881 ccagcaccat tgctttcatc aggtattgcc caggcgccgt ttgaagttct ggatttcgtc
     8941 gaccggcaaa ggatcgactc cgcgcaggga ggcaacctcg accgagacca ggtcaccgaa
     9001 catcaccgac cagaacaggc gttccgatcg tgtctcgcca agggtctcca cctcgaagac
     9061 ctgggcgccc gtcccctcga tcgattcggc ggtgagcttc atccgccgcc gttcgcgggc
     9121 cgactggccc gaatcggtga ggaatacggc tcccagccct ccggacccct tcgaccacgc
     9181 ttccatcagg ttgtggtttg cctcggggat ctcggccgag aatgcgagct gtttggcgtt
     9241 ttcgttgaac tggttggccc agcgcctggc cggggccgta gtgaggcttg ctccatgcac
     9301 gacaacggga gtgtccccta tttgggccgc gagtgctctc gcgagatgct ccatgtcacc
     9361 cttgacggtg tccaggaagt ccgcggcctg ctcgagctca cggcggacat caggcgccac
     9421 gcccgccagg tgtgcaatgt aggtcgatac gacagtcatg tacccgaccg tcatccgggg
     9481 ctggaagaag ccgggcaggc cgactactcc tatgccggcg tcgcgagccc ttgctccgag
     9541 ctctccaccc gtcccg
//
LOCUS       DAOQFI010000002         8678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_2731_length_8678_cov_3.246086, whole genome shotgun sequence.
ACCESSION   DAOQFI010000002 DAOQFI010000000
VERSION     DAOQFI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2731_length_8678_cov_3.246086"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="PLE93_00050"
     CDS             <1..1716
                     /locus_tag="PLE93_00050"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNH85465.1"
                     /translation="GSALRTIGIGVNGYSDPATYEGGRKNYVPGRTTRALKPGPIEAG
                     QWAVEFGAGWIDPDGAGVDWKLEVITSTAPEWSNDAFTADPYAPYSANPNPGWYTGDL
                     HAHGEMEPGNATMKQTMDLGFGALPAGNGLDFMTVVDHNNSNSLGVLGEDQYSYPGKL
                     VIPGIEVTTYNGHWNAIGSSNFADFRFSDVLRWDDAGDDNIQTDGELSTVRPAIQPAS
                     QMQPILDGGGYTQVNHPETLKAAPAQCRGCAWTYSDAQTDWSKVTALEIQNGPAGIPA
                     GSPTAMNPFSVDAIKTYERLLADGFHIAAVGSSDDHQGGEATGAFDSPVGRGATVVHA
                     DQLSTQGVIDAVKAGHTYVKPFGADAPDVELSASTPQGGKAIPGDSVTGSSLAIKIAV
                     EGAASAVRPGPYTLKLLQDGIEVDSVTVTGDSYSHTFNVSESGRYGFELTRPLGDQTM
                     IEAYSTPVWFTFKKPSNAFSFGAYKANRAKGTATLKVKVVSPGSVKLTGPGLNNASAK
                     VTKANQTVTLNLKPKASLKKQLKKKGSAKVKVKVTNTPTGGSAASKSKTVKLLGKKAK
                     KKKRH"
     gene            1762..2694
                     /locus_tag="PLE93_00055"
     CDS             1762..2694
                     /locus_tag="PLE93_00055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNH85466.1"
                     /translation="MSGSADPKDQHKDATGSAIAGLPVVFGIWLVSVSFVFFPLSLLL
                     TDWLGDPAGATPGEMLGDPLFLSIFLAVTGLWTLFAPLALTGWADRTVWMKRLGCREL
                     TPEERSRLDPLWREVFLYAGIHPEAYRLKMSDKPVQNAFAVGDGMITLDQGILSMDDA
                     DLRAIIGHELGHHVRRHTQINMLALWFGAPARILYGILLFLVAGLIRLAGALSGCSWA
                     IIVLLVAFALAVPAIVLFLLLGIAQLLTRLLDRKSEFEADLYSAGIGYRQEMIGALTE
                     LREIYGDPEPNKPWSPARLGSTHPPFTDRIAALG"
     gene            complement(2702..3079)
                     /locus_tag="PLE93_00060"
     CDS             complement(2702..3079)
                     /locus_tag="PLE93_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85467.1"
                     /translation="MIPALAGVALLLAAAGIGFGIGKTTASRDPGPDLTRAEAFVQAR
                     KKAEKEVSRQMARRGFDAGRRSGHSHGIIAGGMAAESAVTIQFRQQRASVAQSDAAEV
                     QSELAGMTAAPAPPVPTPEPDDG"
     gene            complement(3114..3635)
                     /locus_tag="PLE93_00065"
     CDS             complement(3114..3635)
                     /locus_tag="PLE93_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85468.1"
                     /translation="MNYDRISPWLISGVLAVLMAGLAGWAIGAGAANGESDASAARKD
                     GFREGFALVFPDARSATAGRGFKEGVHRGRTAGAKTGSREGATIGAGNVEIEQAVDSE
                     KAAEAAASAAQSEIAARQPNCGIVPDAPSWCPTSDELASYKAAVKAAEEAAKEAEKQK
                     QEDQKPGKANGFP"
     gene            complement(3632..4465)
                     /locus_tag="PLE93_00070"
     CDS             complement(3632..4465)
                     /locus_tag="PLE93_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85469.1"
                     /translation="MRPPRKPTRRRGPDPAPEDTQEMFAAGQAVPGEPAGDEETREYE
                     TVATGEGEPVDREEAIEPVEEEEETAEALDEAETGVIEEDTEVIEEEDTEVIEEDDTE
                     VIEEEAWDDEGYEDWEQEAEEVEEEPDPEAKAEPENSGGFAVWFTGFRQRANEVWSGF
                     WGRIGSIKLPRHELNGRKVLAVAGVLAIALMVGAGGYLLGKGSGDDVDQARLEGEFAG
                     RKAGAIAGATRGYAAGFKKGRDLAFRKSYSASYKRNYIRAYQDAGMDPPKPEDVEVPE
                     P"
     gene            4545..6824
                     /locus_tag="PLE93_00075"
     CDS             4545..6824
                     /locus_tag="PLE93_00075"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNH85470.1"
                     /translation="MGLCAGGVREGCRTVVAALAMLAFTVALAGPARANETVFGGELT
                     CVTQPGGVRQCAGPGGPDTNAPADTVPSFDGTPIDINFALPDKSRFGPPYPLAMYFHG
                     FGGEKEPFGGYLKRFTDRGIAALGMTERGFKESCGSEVAIEALDADTPGACDEGFIHL
                     MDSRYEVRDAQYFAGILADEGLIHPQKIGVVGGSYGGGKALALATLKNRVMLPDGSLV
                     PWKSPAGKSMAIAVAAPIVPWSDMAYALAPNGGTLDYASSSPYRKPYGVMKSGIVNGL
                     FATGENFSGSYGAPVDPLFDVIGWKDELAAGEPYGNDPLIATAVGELTRFHSAGYIDD
                     SVTPAPMFIAQGLTDDIFPVDEAIRYYNRIKASHPDARIGLYLADIGHPRALLATQGR
                     PADIQIADARVEEWFAHYLLGDGPEPETTVVARSQVCPYEEPSGGPFTADTWAKLAPG
                     EVRISDPGRHVIEATSGDDGVAAGFISILPGCSQMPDTAEPGTWSRDFPAPGGDGYTV
                     AGSTTVIADISSPNGGESEIAARLLEVTGGQERLIGRALYRPARSGRQVFQLHANVYA
                     IGPDAHLRLQLLPRDGMTGPSAAASYGRPSSDQRDITISDIEVRVPVAEHPGEAGGQV
                     RQPLRKVLPAGRSLAAQFRPTGAARPTGAAQVDRLRIAGRRLSLRVRCRADTDRCLSG
                     RLVIRGYRRGGPVGRGLIASHRLPRMVTGHGRTYRLPLQRKLLERLRRLDNEKVRVRV
                     TVTVPGSREESTLLIFLAG"
     gene            complement(6843..7418)
                     /locus_tag="PLE93_00080"
     CDS             complement(6843..7418)
                     /locus_tag="PLE93_00080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNH85471.1"
                     /translation="MDDDLTRGDGEDEGLSRRPDPEDLDPTRIKLRPVAVDHVGVLRW
                     IHERPEVSRWWDEPGEDFPWDEPDSTRLTIFVDGQIGGMIQYWEEDEPKYRHAGIDLF
                     IDPALHNKGIGTTAVREVARYLFEARGHHRLEIDPAAENLAAIRVYEKVGFKPVGLTQ
                     RAERDADGGGWHDSLLMDMLSTELLPAEQED"
     gene            7507..7854
                     /locus_tag="PLE93_00085"
     CDS             7507..7854
                     /locus_tag="PLE93_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNH85472.1"
                     /translation="MGQHFLKPLFDRVVIKELEPDRMRNSGLVVPPGTSEPPPQHGIV
                     LAVGQGIDWWEAAGVRMPVRPGDHVVFPAQAGNWVEVDEERLLVCRVTELLGVLENAR
                     DPETPELGGEVLD"
     gene            complement(8046..>8678)
                     /locus_tag="PLE93_00090"
     CDS             complement(8046..>8678)
                     /locus_tag="PLE93_00090"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNH85473.1"
                     /translation="GHSEGGQAALFTAEIGLRRAPGLSFRGVSAFAPASHVSDFVELA
                     RSFDTSSPLTDGFSGLGSLILEGAAIAEPSLWQEYRDGGLSAEALELMPDTQRLCLAD
                     LSGTGSFGSMPPGALEGPDGPQVLPEIYAVLDRNDPRTLSLPGVPVRLDQGSKDLVVW
                     QSQTAEVADVLESKGAEVSYEVWPGADHTDITDLDYAASASADWLIDHLR"
BASE COUNT         1403 a         2985 c         2828 g         1462 t
ORIGIN      
        1 ggcagcgcac tgcggactat cgggatcggc gtgaacggtt actcggatcc ggctacctat
       61 gaaggcggcc gcaagaacta cgtgcctggc cgcaccaccc gcgcgttgaa gcccggcccg
      121 atcgaagcgg ggcagtgggc ggtcgagttc ggtgccggct ggatcgaccc ggatggtgcc
      181 ggggttgact ggaagttgga agtaatcacc agcaccgcac cggagtggag caacgacgcc
      241 ttcaccgccg atccgtacgc gccctacagc gcgaacccga atcccggctg gtacaccggt
      301 gacctgcacg ctcacggtga aatggaaccc ggtaacgcga ccatgaagca gacgatggat
      361 cttggtttcg gagcgctgcc cgccggcaac ggactcgact tcatgaccgt ggtcgaccac
      421 aacaacagca actcgctcgg ggtgctcggt gaggaccagt acagctaccc gggcaagctg
      481 gtcattccgg gcatcgaggt caccacgtac aacggccact ggaacgcgat cggttcgagc
      541 aacttcgccg acttccggtt cagtgacgta ctgcgttggg acgacgccgg cgacgacaac
      601 atccagaccg acggcgaact cagcacggtt cgccccgcga tccagcccgc ctcgcagatg
      661 cagccgatcc tcgacggcgg cggctacact caggtcaatc acccggagac cctgaaggct
      721 gcgccggccc agtgccgcgg ctgcgcctgg acctactcgg acgcgcagac cgactggagc
      781 aaggtcaccg ccctggaaat ccagaacggc ccggccggga ttccggccgg ttcgccgacc
      841 gcaatgaacc cattctcggt cgacgcgatt aagacctacg agcggctgct cgcggatggg
      901 tttcacatcg ccgcggtcgg ctccagtgac gaccaccagg gtggcgaagc gacgggagcc
      961 ttcgattccc cggtcggccg gggagccacc gtggtccacg ccgatcagct ctcgacccag
     1021 ggcgtgatcg acgcggtcaa ggccgggcac acctacgtga agcccttcgg cgccgatgcc
     1081 cccgatgtcg agctctccgc ctcgaccccg cagggtggca aggcgattcc cggtgattcg
     1141 gttaccggat cgagcctcgc aatcaagatc gccgtcgagg gcgcggccag cgcggttcgc
     1201 ccaggtccct acacgctgaa gctgctgcag gacggcatcg aggttgattc cgtcacggtc
     1261 accggtgaca gttacagcca caccttcaac gtctccgagt cgggccgcta cgggttcgag
     1321 ctgacccggc ccctcggcga tcagacgatg atcgaggcct actcgacccc ggtctggttc
     1381 accttcaaga agccgtcgaa tgccttcagc ttcggcgcct acaaggcgaa ccgggccaag
     1441 gggaccgcga ccctcaaggt caaggttgtg agccccggca gcgtcaagct gaccggcccc
     1501 gggttgaaca acgcgtcggc caaggtgacc aaggcaaacc agacggtgac cctgaacctg
     1561 aagcccaaag cttcgctgaa gaagcagctg aagaagaagg gctcggccaa ggtgaaggtc
     1621 aaggtgacca ataccccgac cggcggatcg gccgcctcga aatcgaagac ggtcaagctg
     1681 ctcggcaaga aggcgaaaaa gaagaagcgg cactagccgg ggccggccca ggggcagcca
     1741 gcgcaacccc gggatcgacc ggtgagcgga tcggccgatc cgaaggatca gcacaaggac
     1801 gcgacggggt cggcgatcgc cggcctgccg gtcgtgttcg ggatctggct ggtctcggtt
     1861 tccttcgtct tcttcccgct ttcgctgctt ctgaccgact ggcttggcga cccggccggg
     1921 gcgacgccgg gcgaaatgct gggcgacccg ttgttcctgt cgatattcct ggccgtgacc
     1981 gggctgtgga cgctgttcgc gccgctggcc ctgaccggct gggcggatcg caccgtctgg
     2041 atgaagcgac tcggctgccg ggaactgacc ccggaagagc gatcccggct cgacccgctc
     2101 tggcgggaag tcttcctcta cgccggcatc catccggagg cctaccggct gaagatgagc
     2161 gacaagccgg tacagaatgc gttcgcggtc ggcgacggca tgatcaccct ggaccagggg
     2221 atcctcagca tggatgacgc ggacctccgc gcgatcatcg gccacgaact tggccaccac
     2281 gtccggcgcc acacccagat caacatgctc gctctctggt tcggtgctcc ggcccggatc
     2341 ctctacggca tcctgctttt cctcgtcgcc ggattgatcc ggctcgccgg ggccctttcc
     2401 ggctgcagct gggcgatcat cgtgctgctg gtcgccttcg cgcttgcggt tccggcgatc
     2461 gtcctgttcc tgctgctcgg catcgcccag ctcctgaccc ggctgctgga ccgcaagtcc
     2521 gagttcgaag ccgacctcta ctcggccggg atcggctatc gccaggagat gatcggagcc
     2581 ctgaccgaac tgcgtgaaat ctacggtgac ccggaaccca acaaaccgtg gtcaccggcc
     2641 cgcctgggct ccacccaccc gcccttcacc gatcgaatcg ccgccctggg ctgagccggg
     2701 gtcagccgtc gtcgggttcg ggagtcggga ccggcggcgc gggggcggcg gtcatcccgg
     2761 cgagctctga ctggacctcg gctgcgtcgg actgggcgac cgacgcgcgt tgctggcgaa
     2821 actggatagt cacggcggat tcggcggcca tcccgccggc gatgatcccg tggctgtggc
     2881 cggaacggcg gcctgcgtcg aagccgcgcc gggccatctg gcggctcacc tccttttccg
     2941 ctttcttccg ggcctgaacg aaggcctcgg cccgggtcag gtcaggcccg ggatcacggg
     3001 aagcggtggt cttgccgatc ccgaaaccga tccccgccgc ggcgagcaac agggcaacac
     3061 cggccagggc gggaatcatc cagggccttc cccccggttc gctttcttcc ggttcagggg
     3121 aatccattgg ccttgcccgg cttctgatct tcctgctttt gcttctctgc ttccttggct
     3181 gcttcctcgg ctgccttgac cgcggccttg taggaagcga gttcgtcact ggtcgggcac
     3241 caggaaggcg cgtcggggac tatcccgcag ttcggctggc gggccgcaat ctccgactgg
     3301 gccgccgacg cggccgcctc ggcggccttc tccgaatcga ccgcctgctc gatctcgacg
     3361 ttgccggcgc cgatcgtggc tccttcccgg gagccggtct tcgcaccggc cgtccttccc
     3421 cggtggacgc cctccttgaa cccccgaccg gcggtggccg accgtgcgtc ggggaagacc
     3481 agcgcgaatc cttcccgaaa accgtccttg cgggcggcgc tggcgtcact ctcgccgttt
     3541 gccgcccctg ccccgatagc ccagccggcc agcccggcca tcaggacggc cagcacgcct
     3601 gagatcagcc agggggagat ccggtcgtag ttcatggttc ggggacttcg acgtcttccg
     3661 gcttcggcgg gtccatcccg gcgtcctggt aggcccggat gtagttgcgc ttgtaggagg
     3721 ccgaatatga cttgcggaag gcgaggtcac gacccttctt gaaaccggcc gcgtagcccc
     3781 tggtcgcccc ggcgatggcg ccggctttgc ggccggcgaa ctcgccctcg agccgggcct
     3841 ggtcgacatc gtcgccggaa cccttgccga gcagatagcc accggcaccg accatcagcg
     3901 cgatcgccag aacccccgcc acggcgagga ccttccgtcc gttcagctca tgccggggca
     3961 gtttgatcga accgatccga ccccagaatc cggaccagac ctcgttcgcc ctctgcctga
     4021 aaccggtgaa ccagaccgcg aaaccacctg aattctccgg ttcggccttc gcctccggat
     4081 ccggttcctc ttctacttcc tctgcttcct gctcccagtc ttcgtatccc tcgtcgtccc
     4141 aggcttcctc ttcgattacc tcggtgtcgt cttcctcgat tacctcggtg tcttcctctt
     4201 cgatcacctc ggtgtcttct tcgattaccc cggtctcggc ctcatcgaga gcttcggcgg
     4261 tctcttcttc ttcttcgacc ggctcgatgg cctcttcccg atctaccggt tccccttcgc
     4321 cggtcgcgac ggtttcgtac tccctggttt cctcgtcccc ggcgggttcc ccgggcacgg
     4381 cctgccctgc ggcgaacatc tcctgcgtgt cctcgggggc agggtcggga ccgcgtcttc
     4441 tggtgggctt tcttggggga cgcatcgaca aaatcctcag tcgggatacc caatgattac
     4501 ccaagtacac ctgaatgccc ttccctcagg tctagaattg cggcatgggc ctgtgcgcgg
     4561 gaggagtgcg ggagggatgc cggacggttg ttgccgccct ggcgatgctt gctttcacgg
     4621 tggcgctggc cggccccgcc cgggccaacg aaaccgtgtt cgggggagag ctgacctgcg
     4681 tgacccagcc cggcggagtg cgccaatgcg ccggtcccgg cgggccggac accaacgcgc
     4741 cggctgacac ggtcccgagt tttgacggaa ccccgatcga catcaacttc gccctgcccg
     4801 acaagtccag gttcggcccg ccctatccgc tggccatgta cttccacggt ttcggcggcg
     4861 agaaggagcc cttcggcggc tacctcaaac gattcacgga ccgcgggatc gccgcactcg
     4921 gcatgaccga aaggggattc aaggaatcct gcggcagcga ggtcgcgatc gaagcgctcg
     4981 atgccgacac gcccggggcc tgcgacgagg gcttcatcca cctgatggac agccgctacg
     5041 aagttcgcga tgcccagtac ttcgccggga ttctggccga cgagggcctg atccatccgc
     5101 agaagatcgg ggtggtcggc gggtcctacg gcggaggcaa ggcgctggcg ctggccacgc
     5161 tgaagaaccg ggtcatgctg ccggacggca gcctcgtgcc gtggaagagc ccggccggca
     5221 agtcgatggc gatcgcggtg gccgccccga tcgtgccctg gtccgacatg gcctatgccc
     5281 tggctccgaa cggcggcacc ctcgactacg cgagcagctc tccctaccgc aagccttacg
     5341 gggtgatgaa gtccggaatc gtcaacggct tgttcgctac cggcgagaac ttcagcggca
     5401 gttacggagc cccggtcgat ccgctctttg acgtgatcgg ctggaaggac gagctggctg
     5461 cgggcgagcc ctacgggaac gacccgctga tcgccaccgc ggtcggcgag ttgaccagat
     5521 tccattcggc cggctacatc gacgattcgg tgaccccggc cccgatgttc atcgcccagg
     5581 gactgaccga cgacatcttc ccggtagacg aggcaatccg ctactacaac cggatcaagg
     5641 cctcgcaccc cgacgcgagg atcgggctct acctcgccga catcggtcat ccccgggccc
     5701 tgctggccac ccagggcagg cccgcagaca tccagatcgc ggacgccagg gtcgaggaat
     5761 ggttcgccca ctacctgctc ggtgacggac ccgagccgga gaccacggtc gtcgcccgct
     5821 cccaggtctg cccgtacgag gaaccctcgg gcggaccctt caccgccgac acctgggcga
     5881 agctggcccc cggcgaggtg cgcatctcgg acccgggccg ccatgtgatc gaggccactt
     5941 cgggagacga tggtgtcgcc gccggtttca tcagcatcct gcccggctgc tcgcagatgc
     6001 cggacaccgc ggaaccgggt acctggtccc gggacttccc ggcccccggc ggggatggct
     6061 acacggtcgc cggctcgacc acggtgatcg ccgacatctc ctctccgaac ggcggtgagt
     6121 cagagatcgc ggcccggctc ctggaagtga ccggaggcca ggagcgcttg atcggaaggg
     6181 cgctctaccg tccggcccgc tccggccggc aggtgttcca gctccacgcg aacgtttacg
     6241 cgattggccc ggacgcgcat ctccggctgc aactcctgcc cagggacggg atgaccggcc
     6301 ccagcgcggc ggcttcctac gggcggcctt cgagcgacca gcgggacatc acgatctccg
     6361 acatcgaggt ccgggtcccg gtcgccgagc atcccgggga ggcagggggc caggtcaggc
     6421 agccgctgcg gaaggtcctg ccggccggcc gcagcctggc cgcccagttc cggccgaccg
     6481 gggcggccag gccaaccggt gcggcgcagg tcgatcggct gcgaatcgcc ggccgtcgac
     6541 tgagcttgag ggtgcgctgc cgtgccgaca cggatcgctg cctgtccggc cggctggtga
     6601 tccgcggtta ccgccggggc ggcccggtcg gccgcggcct gatcgccagc caccggctgc
     6661 cgcggatggt gaccggccac ggcaggacct accggctccc gctccagcgg aagctgctcg
     6721 aacggcttcg ccgcctggac aacgagaagg tcagggtgag ggtgaccgtg accgtgccgg
     6781 gcagccggga ggagagcacc ctgctcatct tcctggccgg gtagcgccag aggcgccggc
     6841 ggtcagtctt cctgttcggc cggcagcagc tcggtcgaaa gcatgtccat cagcaagctg
     6901 tcgtgccagc ccccgccgtc ggcatcccgc tcggcccgct gggtcaggcc gaccggcttg
     6961 aagccgacct tctcgtacac cctgatcgcc gcgaggttct ccgccgccgg gtcgatctcg
     7021 agccggtggt ggccccgggc ctcgaacagg tacctggcaa cctcccggac cgcggtggtg
     7081 ccaatgccct tgttgtgcag cgccggatcg atgaagaggt cgatgccggc gtgccggtac
     7141 ttgggctcgt cctcttccca gtactggatc atcccgccga tctggccgtc aacgaaaatc
     7201 gtcaaccggg tcgaatcggg ttcatcccag ggaaagtcct cgcccggctc gtcccaccac
     7261 ctggagactt cggggcgttc gtgaatccag cgcaagacac cgacatggtc cacggcaacc
     7321 ggcctgagct tgattctcgt cgggtcgagg tcctccgggt ccgggcggcg cgagagccct
     7381 tcgtcctccc cgtccccgcg ggtaaggtcg tcatccatga tggccgcagg ctaccggtat
     7441 tggcctctgg gaaccgggcc tgcccgcttc gcaacccgaa tttccggcag cccgtaggct
     7501 tgggtcatgg gtcagcattt tctgaagccg ctcttcgacc gggtggtgat caaggaactt
     7561 gagccggacc ggatgcggaa ctccgggctg gtcgttccgc cgggcacctc cgagccgcca
     7621 ccccagcacg ggatcgtgct ggcggtcggt cagggcatcg actggtggga agcggccggg
     7681 gtgaggatgc cggtccgccc cggtgaccat gtcgtctttc cggcccaggc cggcaactgg
     7741 gtcgaggttg acgaagaacg gctgctggtc tgccgggtca ccgagctgct gggtgttctg
     7801 gagaacgcca gggatccgga aaccccggag ctcggcggcg aagtcctcga ctgaacccac
     7861 gggagagcag agaaacgctt gagcccgatc ccccccggat cgggctcagc gcctccctgc
     7921 ttgttggttc cctcccgttt ggaacccggc tgcctttcca gaggtcagac aaccgctctt
     7981 ataaccggat aagggttcgg gagtttcggt cccgcaaaca aagaagttcg cgattggttg
     8041 aggcctcaac gaaggtggtc gatcagccag tcggcagagg cggacgccgc atagtcgaga
     8101 tcggtgatgt cggtgtgatc ggcaccgggc cagacttcgt aactgacctc cgcccccttt
     8161 gactcaagaa cgtccgcgac ctccgcagtt tgtgactgcc agacgaccag gtcctttgat
     8221 ccctggtcga gccggacggg aaccccgggc aggctcaggg tgcgggggtc attgcgatcg
     8281 aggaccgcgt aaatctcggg cagcacctgc ggcccgtccg gtccctcgag cgcaccgggc
     8341 ggcatcgaac cgaacgaacc ggttccgctg aggtcagcca ggcaaagtcg ctgggtgtcg
     8401 ggcatcagct ccagcgcttc ggcgctgagg ccgccatcgc ggtactcctg ccacaacgac
     8461 ggctcggcga tcgcggcgcc ttcgaggatc agtgacccga ggccggagaa gccgtcggtc
     8521 aggggcgagg aggtgtcaaa ggagcgggcc agctcgacga agtcgctgac gtggctggcc
     8581 ggtgcgaagg cggagacacc gcggaaggaa agcccgggcg cgcgccggag gccgatctcc
     8641 gccgtgaaca gcgctgcctg cccaccctcg gaatggcc
//
LOCUS       DAOQFI010000003         8562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_2784_length_8562_cov_3.574703, whole genome shotgun sequence.
ACCESSION   DAOQFI010000003 DAOQFI010000000
VERSION     DAOQFI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2784_length_8562_cov_3.574703"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1528)
                     /locus_tag="PLE93_00095"
     CDS             complement(20..1528)
                     /locus_tag="PLE93_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH85474.1"
                     /translation="MDRVAESHPRRWAILGVICASLMVVVIDVTVLHVAAPAISEDLD
                     PTALQLLWIIDVYPLVAAPLLIASGVLGDRLGRKRLLVAGMAVFGLASLGATFAPTAE
                     TLIAARALLGVGGAMIMPPTMSIIRDVFRDREERVRAVGIWSAVSAGGAAIGPLIGGF
                     LVEHFWWGAVFLINVPIVLVIIPVAIRLLPESRAAEPPPWDGFAVIMTVIGILGIAFG
                     LKEGARYGFLDPKVLLSLLVGSGSLFWFTLRQLRAPEPLLDVRLFKRREFTAAVGCVF
                     LSMFGLVGIEFFGAQYLQLVLGLEPLEAALRLLPLMLSTLAGGLLAARLLGRLGTRWT
                     ISLGLAGTALGLVPMLALGLTDQYYLLWPSFLVIGFTLEVALVAANDVIISSVPADEA
                     GQAAAIEETAYELGGGFGVAVLGSILAIVYTAKIGPIEGVTGAGIANAEESLARALEI
                     AGKMASRTADPLIEAAKLAFTSGYHTAIAVSIILVGASAGLAAVLLRPRTPQ"
     gene            complement(1575..3284)
                     /locus_tag="PLE93_00100"
     CDS             complement(1575..3284)
                     /locus_tag="PLE93_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85475.1"
                     /translation="MTGRRTILFSLIALTMGLGLGAQAQAAKPKADVVFSNGGRILTM
                     KADGTERKVLFGKDRLPRNDGLGAVEPSVSPDGTKITFGFRRLDRYDSLFDVWVMNFD
                     GTGARKLLASNRKQRYADPEFTAGGRIIAASFRENSSRADARIFTMDTNGKSRKVMLR
                     LKQRQRPWEGWKSLAEPSLSPDGKHLLYLLDPGYDGTSFEEGYGASLRVLNIDTGKSR
                     EIAGNSLGGDFSPGGNRIVFSEVDSEGDNDFCWSYDYSCINFSRIRLVNLNGTGARDL
                     TSLDRRAADERRPDWSAPGRIVFQSTRGPEREVAETTEIWSIRPGGECLARLTNGSPA
                     SLSPVLVDPEGKLTGPRGCNARPPGPNRELTAPDMSGSAIDNLWLGRSHHNVLLSNVE
                     AERNRSTFTYADCGVIPFSGCGRGVLIYNEDICSLQGYTAAYIGSDWAQKQRGIPVLR
                     SLKPGRETPAFTAGFTGRTAFFIIGGSGLGDRKLQHRKEIDALRAVGSPLATGDLPKP
                     RIPVSDIRAMKRVQRVYKRTGSVARTARLLDRGNYFVRSNLKFARIFKAGGYGRINCP
                     GAD"
     gene            complement(3287..4888)
                     /locus_tag="PLE93_00105"
     CDS             complement(3287..4888)
                     /locus_tag="PLE93_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85476.1"
                     /translation="MRLRFLAGLLAGMLGCLVLWPGAGQATTPPLAPLAEIVFENGGR
                     IVSIKADGSGRKVLTRKGTDLLRRNYLADIHYFGDSQPAISPDGEKLAFVRYRPAAHS
                     QQVMLADRDGWGATAVKGINAEIQSLDFMPDGRLLLTTWDYRYGYSRTTETFRILALD
                     PATGKKKVLVSKRFFGNYNDDTLQAQEVSPDGKSLLYTRDFGENDDGYDVVQLRIRNL
                     VSGAERVLVTGAEAGSFSPDGERVAFTKMCVDTEGYAHCAHSGDGRTGVWTIGVDGSD
                     RQLIVTGDGGYSSPDWGPGGGYIIFASSRNFPEERQDANEIYSVRSDGECLTWLTNGS
                     PESVMPAWAPETEVRTDPGAKCGAAGRKPLAEARPPRAIDTDPRPRYWAGPIVANRLL
                     SKVDGYAQDEFGSPIYRYGDCPFFDSSDCRRPITIEIAFACGGLGEVRVPPRGREQVI
                     RRRGVRVTRHWTARGKFVTTTLRTGLVDIGIGQSMLDVEDIGGRASTFSDHLAFIDNL
                     RPVGKSLDPGSKLPPAAMVKCQGRR"
     gene            complement(4885..6768)
                     /locus_tag="PLE93_00110"
     CDS             complement(4885..6768)
                     /locus_tag="PLE93_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85477.1"
                     /translation="MSTSGGRDNHHNRWPASLIALFAAALLLAAFLLAADRAAAIPPE
                     QLPAATIVFENGGRIVSIKADGTGRKVLTPRRAKTMIETGIRDSDPAVSPDGQTVLFS
                     RDDDSSGSVDRSKLGLFSIDVDGGTPTQIRATEEIETLQLTTYTGGFAWAPDGSRIYE
                     VEYIVKLVPAGWPYAVRTRIISMLPDGSDVKVIEVGKTTFKNEKPSGDLWIASEVEPS
                     PDGSELLVTRIPMNGHAQPRIDALDLSTRKSKVIEKSAFDPSFSPDGQRIVFSTTRDK
                     VNESCYEGSCSSSQQLYLMDADGSNPRLLLGKVQPWNFAEPDWSGDGSRILLTSNKAS
                     VTTIFGDEIWSVEPDGSCLSRITNGSPASESGSWGLTPDTSPTGCGLGMGSRGVAEVE
                     PPAKIADLEVTPLWLGRNFGLLLFSDYFVDRSNASFTYWDCSWFRNRCPATIAIGSAH
                     ACERNLEGELGNGAYQGMVRRKGGLAVMTGSVRPSLSTIFLADGREVWITSPWRHDGK
                     ATSRAWHMKAIDHLRPYGASKPPASFEAPVFAWHTVHTARVMVRSYRRTQSLKKAAVR
                     AGIFRAGMGISFSYSRRAAQAWLRFSRDLARLGPVKSVECRRWANKPGTIDVRSDFRE
                     LIR"
     gene            complement(6930..7003)
                     /locus_tag="PLE93_00115"
     tRNA            complement(6930..7003)
                     /locus_tag="PLE93_00115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6967..6969),aa:Pro,seq:cgg)
     gene            complement(7055..7774)
                     /locus_tag="PLE93_00120"
     CDS             complement(7055..7774)
                     /locus_tag="PLE93_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85478.1"
                     /translation="MSSAFRQRRPGQFRTLIILVVAIGALLIALVGMAIQKPDNEYEN
                     VQGKQDMRQIFGGVRQLDDRLGSDDAPAQMQVFLDAQSSTYKDQFLDTIPALVSGPVR
                     DGTLKLLYRNRSLTRNATERSFYGIEAAMKQGYGWQYAYLMFRNQDLAKEQGIDDDFL
                     TTLAESIDNLEIEQWKQDYQDGLEEGSAMNADLQAQDQIAIDLKIRDAPAAVVNGPSG
                     TEILQDAPDLAEMQAAIGEVQ"
     gene            complement(7776..8039)
                     /locus_tag="PLE93_00125"
     CDS             complement(7776..8039)
                     /locus_tag="PLE93_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85479.1"
                     /translation="MAEEPETEVAAASDPFIESLMDTDLYSMGAYFSDRNPELIDELI
                     DRSVAIEERGLVRFSEEEDMELEQAFKTLITGLAVRYYKSVTG"
     gene            8085..>8562
                     /locus_tag="PLE93_00130"
     CDS             8085..>8562
                     /locus_tag="PLE93_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNH85480.1"
                     /translation="MADRNEIIAFCDELLEIESFGDYGPNGLQVPGKAVIGKVATAVS
                     ANLASIEAALATGVDLLLTHHGLFWDFHPRALTGAMARRLRLALGADLNVAGYHLPLD
                     GHPEIGNNALIVRQLGFEPVPGEIGEAKGRHIGQIGRRAEPIPATALFELVGRELGR"
BASE COUNT         1489 a         2786 c         2800 g         1487 t
ORIGIN      
        1 cacctgccgg attgcggggc tactgcggcg tccgggggcg cagcaggacc gcggccagac
       61 cggcgctggc gccgaccaga atgatcgaga ccgcgatcgc ggtgtggtag ccggacgtga
      121 aggcaagctt ggccgcctcg atcaagggat cagccgtccg gctcgccatc ttgccggcga
      181 tttcgagcgc ccgtgccagc gactcctcgg cgttggcgat cccggccccg gtgacgccct
      241 cgatcggacc gatcttcgcc gtgtagacga tcgccaggat cgaaccgagc acagccacgc
      301 cgaaaccgcc tccgagttcg taggccgtct cctcgatcgc cgccgcctgg ccggcctcgt
      361 cggccgggac cgaggaaatg atcacgtcat tggcggcgac cagggccacc tccagggtga
      421 agccgatcac caggaaggaa ggccagagca ggtagtactg gtcggtcagg ccgagcgcca
      481 gcatcggaac aagaccgagt gcggtgccgg ccagcccgag tgaaatcgtc cagcgggtgc
      541 cgagcctgcc cagcaggcgc gcagcaagca agcctccagc cagggtggaa agcatcagcg
      601 gcaagagccg gagcgccgcc tccaagggtt cgaggcccag caccagctgc aggtactggg
      661 ccccgaagaa ctcgatcccg accagtccga acatggacag gaagacgcaa ccgaccgccg
      721 ccgtgaactc ccgccgtttg aagagccgca cgtcaagcag gggctcaggc gcccgcagct
      781 ggcgaagggt gaaccagaaa agggatccgg aaccgaccag aagcgagagg agcaccttcg
      841 gatcgaggaa gccgtagcgg gcaccttcct tgaggccgaa ggcgattccg aggattccga
      901 tcacggtcat gatcaccgcg aagccgtccc agggcggcgg ttcggccgcc cggctttcgg
      961 gcagcagcct gattgcgacg gggatgatca caagcacgat cggcacgttg atcaggaaca
     1021 cggcacccca ccagaaatgc tcgaccagga aaccgccgat cagcgggccg atcgcggccc
     1081 cgccggcgct gaccgcgctc cagatcccga ccgcccgcac ccgctcctcc cggtcgcgga
     1141 agacatcccg gatgatcgac atggtcgggg gcatgatcat cgcgccgcca accccgagca
     1201 gggcccgggc cgcgatcagg gtttccgcgg tcggggcgaa ggtcgccccg agagatgcca
     1261 gcccgaagac ggccatgccc gcgaccagta gccgtttgcg accgagccgg tctcccagca
     1321 ccccggatgc gatcaggagc ggcgccgcga ccagcggata gacgtcaatg atccagagca
     1381 gctgaagcgc cgtcggatcg aggtcttcgg aaattgccgg cgccgccaca tgcagcacgg
     1441 tcacgtcgat caccaccacc atcagcgagg cgcagatcac cccgaggatc gcccagcggc
     1501 gaggatgtga ttcagcgacc cggtccatga tcaccagatg aaatcaggag atggcttgcc
     1561 acccccgggc tggttcagtc tgccccgggg cagttgatcc tgccgtagcc accggctttg
     1621 aagatgcggg cgaacttcag gttgctgcgg acgaagtagt tgccgcggtc gagcagcctg
     1681 gcggtacggg cgaccgaacc ggtgcgcttg tagacacgct gaacccgctt catcgcacga
     1741 atgtccgaga ccgggatccg cggcttcggc aggtcgcccg tggcgagcgg cgagccaacg
     1801 gcccgcaagg catcaatctc cttgcggtgc tggagtttgc gatcaccgag ccccgagccc
     1861 ccgatgatga agaaggccgt gcggccggtg aagccggccg tgaaggccgg agtctcgcgg
     1921 cccggtttga ggctgcgaag caccgggatg ccccgctgct tctgggccca gtcggaaccg
     1981 atgtatgccg cggtgtatcc ctgcagggag cagatgtcct cgttgtagat caaaaccccg
     2041 cggccgcagc cggaaaaggg aatgacgcca caatcggcgt aggtgaaggt cgaccggttc
     2101 cgttcggcct cgacattcga gagcagcacg ttgtggtggc tgcgcccgag ccacaggttg
     2161 tcgatcgccg acccggacat gtcaggggcg gtgagctccc ggttcggtcc cggtggtctg
     2221 gcgttgcatc cccgcggccc ggtcagcttg ccctcggggt cgaccagcac gggcgagagg
     2281 ctggccggcg agccgttggt caggcgggcc aggcattcgc cgcctggccg gatcgaccag
     2341 atctcggtcg tctcggcgac ttccctttcc ggaccacggg ttgactggaa gacgatgcgg
     2401 cccggagcgg accagtccgg ccggcgctcg tcggcggctc gcctgtcgag gctggtcagg
     2461 tcgcgcgcac cggttccgtt caggttgacc aggcggatcc ggctgaagtt gatgcacgaa
     2521 tagtcatagc tccagcagaa gtcgttgtct ccttcactgt caacctcact gaagacgatc
     2581 cggtttcccc cgggcgagaa gtccccgccg agcgagttcc cggcaatctc gcggctcttg
     2641 ccggtgtcga tgttcagcac ccgaagcgaa gccccgtagc cctcctcgaa agaagtcccg
     2701 tcgtatcccg ggtcgagcag atagagcaga tgcttcccgt cgggcgagag cgaaggttcg
     2761 gccaggctct tccagccctc ccagggacgc tggcgctgtt tcagccgcag catcaccttg
     2821 cgggatttgc cgttcgtatc catggtgaag atccgggcgt cggcccggct ggagttctcg
     2881 cggaaggagg cggcgatgat ccggccacct gccgtgaatt ccggatcggc gtagcgttgc
     2941 ttcctgttcg aggccagcag cttgcgggct ccggtgccgt cgaagttcat cacccagaca
     3001 tcgaacagag agtcatatct gtcgagccgc ctgaagccaa aagtgatctt tgtcccgtcc
     3061 ggggaaaccg aaggctcgac ggcgccgagc ccgtcgtttc ggggaaggcg gtccttgccg
     3121 aagaggactt tccgttcggt gccgtcggcc ttcatcgtga ggatgcggcc gccgttgctg
     3181 aacaccacgt ccgccttcgg cttcgccgcc tgagcctggg cgccaaggcc gaggcccatg
     3241 gtgagggcga taagcgaaaa gaggatcgtg cgacggccgg tcatcttcac ctccggccct
     3301 ggcacttgac catggcggcg ggaggcagct tcgaaccggg gtcgagggac ttgccgaccg
     3361 gtcgcagatt gtcgatgaag gccaggtggt ccgagaaggt cgaggccctg ccgccgatgt
     3421 cctcgacgtc gagcatcgac tggccgatcc caatgtcaac caggccggtc cgcagggtgg
     3481 tagtgacaaa ttttcctcgc gctgtccagt gccgggttac cctgactccg cgtcgccgga
     3541 tcacttgctc cctgcctcgg ggtggcaccc tgacctcgcc gaggccaccg caggcgaagg
     3601 caatttcgat cgtgatcggt cgccggcaat ccgagctgtc gaaaaaggga cagtcgccgt
     3661 agcggtagat cggggaaccg aactcgtcct gggcgtaacc gtcgaccttg gagagcagcc
     3721 ggtttgcgac gattggcccg gcccagtagc gcgggcgggg gtcggtgtcg atcgccctcg
     3781 gcggccgcgc ctcggccagc ggtttgcgtc cggccgcacc gcatttcgca ccggggtcgg
     3841 ttcgcacctc ggtctccggc gcccaggcgg gcatgaccga ctccggtgat ccgttggtca
     3901 gccaggtcag gcattcgccg tcggagcgca ccgagtagat ctcgttcgcg tcctgccgct
     3961 cctccgggaa gttccggctg ctggcgaaga tgatgtagcc gccgcccggg ccccagtcgg
     4021 gcgagctgta cccgccgtcc ccggtcacga tcaactggcg atcggaaccg tcgaccccga
     4081 tcgtccagac cccggtcctt ccgtcaccgg aatgagcgca gtgggcgtaa ccctcggtgt
     4141 caacgcacat cttcgtgaag gcgacccgct cgccatcggg agagaacgag cccgcttcgg
     4201 cgcccgtcac cagtacccgc tctgccccgc tgaccaggtt ccggatccgc agctggacca
     4261 cgtcgtaacc gtcatcgttc tcgccgaagt cacgggtgta gagcagcgac ttgccgtcgg
     4321 ggcttacttc ctgggcctgg agcgtgtcgt cgttgtagtt gccgaagaac cttttcgaga
     4381 cgaggacctt tttcttcccg gtcgcggggt cgagcgcgag aatccggaag gtctcggtgg
     4441 tccggctgta gccgtagcgg taatcccagg tggtgaggag gagccggccg tcgggcatga
     4501 agtccagcga ctggatttcg gcgtttatgc ccttgaccgc ggttgccccc caaccgtccc
     4561 ggtcggccag catcacctgc tgcgaatgtg cggcgggccg gtacctgacg aaggcgagtt
     4621 tttcgccgtc cggggaaatc gccggctggc tgtcgccgaa gtaatggatg tcggccagat
     4681 agttgcggcg gagcaggtcg gtgcccttgc gggtcagaac cttgcgtccg gatccgtcgg
     4741 ccttgatcga aacgatccgg ccaccgttct cgaaaacgat ctccgccagc ggcgccagcg
     4801 gcggcgtcgt cgcctgcccg gcccccggcc aaagcaccag gcacccgagc atcccggcga
     4861 gcagcccggc caggaaccgc aacctcaccg aatcagctcc ctgaagtccg agcggacatc
     4921 gatggtgccg ggtttgttgg cccagcgtct gcattcgacc gacttgacgg gtcccagccg
     4981 ggccagatca cggctgaacc tgagccaggc ctgcgccgct cgcctcgagt agctgaagct
     5041 gatgcccatc ccggcccgga agatccccgc ccgaacggcc gccttcttca gggattgagt
     5101 ccgtcggtag ctgcgcacca tcacccgcgc ggtgtgcacg gtgtgccagg cgaagaccgg
     5161 ggcctcgaag ctggccggcg gcttgctcgc cccgtacgga cgcagatggt cgatcgcctt
     5221 catgtgccag gcccgcgaag tcgctttgcc gtcatgtcgc cagggcgagg taatccagac
     5281 ctcacgacca tcggcgagaa agatggttga aagcgacggc ctgacgctgc cggtcatgac
     5341 tgccagtcca cccttgcgac ggaccattcc ctgataggcc ccgtttccga gctctccctc
     5401 gaggttgcgc tcgcaggcat gggccgatcc gattgcgatc gtggccgggc aacggttcct
     5461 gaaccaggaa cagtcccagt aagtgaagct tgcgttgctt cgatcgacga aatagtcgct
     5521 gaacaacagc agcccgaagt tcctgcccag ccacagaggc gtcacctcga ggtctgcgat
     5581 tttcgcgggt ggttcgacct ccgcaactcc tctcgaaccc atcccgaggc cgcaaccggt
     5641 cggggaggta tccggggtca atccccagga accggattcg ctggccgggc tgccgttggt
     5701 gattcggctc aggcaagagc catccggctc caccgaccag atctcatcgc cgaagattgt
     5761 cgtgaccgaa gccttgttgc tggtcaggag tattcgcgag ccgtctccgg accagtcggg
     5821 ctcggcgaaa ttccagggct gaaccttccc gagcagcaac cgcgggttcg aaccgtccgc
     5881 gtccatcaga tacagctgct gggacgacga gcagctgcct tcgtaacaac tttcgttcac
     5941 cttgtcgcgg gtcgtcgaaa agacgatcct ctggccgtcc ggtgaaaatg agggatcgaa
     6001 ggccgacttc tcgatcacct tcgatttccg ggtagaaagg tccaacgcgt cgattcgggg
     6061 ctgcgcgtgt ccgttcatcg ggatgcgggt caccaggagc tccgacccgt ccggtgaggg
     6121 ttcgacttca gacgcgatcc agaggtctcc ggatggcttc tcgttcttga aggtcgtctt
     6181 gccgacttcg atgaccttga cgtcgctgcc gtcgggaagc atcgagatga tccgggtccg
     6241 gaccgcatac ggccagccgg ccggaaccag cttgacgatg tactcgacct catagatccg
     6301 cgaaccatcc ggtgcccatg cgaagccgcc ggtgtaggtg gtcagctgga gggtctcgat
     6361 ctcctccgtt gccctgatct gggtcggagt tccgccgtcg acgtcaatcg agaaaaggcc
     6421 gagcttcgac cggtcaaccg atccggatga atcatcgtcc cgcgagaaga gcacggtctg
     6481 cccgtccgga gagacggccg gatccgaatc ccggatcccg gtctcgatca tcgtctttgc
     6541 ccgccgcggg gtcaggacct ttcgacccgt cccgtcggcc ttgatcgaga cgatccggcc
     6601 gccgttctcg aagacgatcg tggccgcggg cagctgctcc ggcgggatgg ccgcggcccg
     6661 gtctgccgcg aggaggaaag cggcaagcag cagcgccgcg gcgaaaagcg cgatgagcga
     6721 tgcgggccac cggttgtggt ggttatccct gccccctgat gtcgacatcc tgcgactcat
     6781 ctttctgttt cccggatccg ttccggtttc cctgtagaag cagggattct accgtccggt
     6841 gacttgaaac tgttacgtgg gtgtgtcctc gcccgtcacg gtaaagctga cggatgacac
     6901 accccgtaac acggtcggat tgagaagtat cggggcgccc ggatttgaac cggggacctc
     6961 accgacccga acggtgcgcg ctaccaggct gcgccacgcc ccgaggaaga atgagcctac
     7021 agaaattgcg gcccggaacc cgtcccggcg gcggctactg gacctcgccg atcgccgcct
     7081 gcatctcggc caggtcgggc gcgtcctgaa ggatctcggt cccggacggg ccgttgacca
     7141 ccgcagcggg ggcgtcgcgg atcttcaggt cgatcgcgat ctggtcctgg gcctggagat
     7201 cggcgttcat ggcgctgcct tcctcgagac catcctggta atcctgcttc cactgttcga
     7261 tctcgaggtt gtcgatcgac tccgccagcg tggtcaggaa gtcgtcgtct atgccctgct
     7321 ccttggcgag atcctggttg cggaacatca ggtaggcgta ctgccagccg tagccctgct
     7381 tcatcgctgc ctcgatcccg tagaagctgc gctccgtcgc attgcgggtg agcgaacggt
     7441 tgcggtagag aagcttgagg gtgccgtccc ggaccggccc gctgaccagg gcgggaatcg
     7501 tgtcgaggaa ctggtccttg taggtcgagc tctgggcgtc gagaaagacc tgcatctggg
     7561 ccggggcgtc atccgagccg agccggtcgt cgagctggcg caccccgccg aagatctggc
     7621 gcatgtcctg cttgccctgg acgttctcgt attcgttgtc gggtttctgg atcgccatgc
     7681 cgaccagggc gatcagcagg gcaccgatcg ccaccacgag gatgatcagc gtccggaact
     7741 ggccgggacg gcgctgcctg aaggcgctgc tcatctcagc cggttaccga tttgtagtac
     7801 cgcaccgcaa gaccggtgat cagggttttg aaggcctgtt cgagctccat gtcctcctct
     7861 tcggagaaac ggaccagacc gcgctcctcg atcgccacgc ttcgatcgat cagttcgtcg
     7921 atcagttcgg gattacgatc ggagaaatag gcacccatcg aatagagatc ggtgtccatc
     7981 agagactcga tgaaggggtc gctggcggca gcgacttcgg tttccggctc ttcagccacg
     8041 gaaccatctt agggacaggg gcgatcccct tatatggtgg ggccgtggcc gaccgcaacg
     8101 agatcatcgc cttctgcgac gaactgctgg agatcgagag cttcggggac tacggcccga
     8161 acggcctcca ggttccgggg aaggccgtga tcgggaaggt cgcgaccgcg gtttccgcca
     8221 atctcgcctc gatcgaggcc gctttggcga ccggcgtcga cctgctgctg acccatcatg
     8281 gcctgttctg ggattttcac ccccgggcac tgaccggggc gatggcccgg cgacttcgcc
     8341 tggccctcgg cgcggacctc aacgtggccg gctaccacct gccgctggac ggacacccgg
     8401 aaatcggcaa caacgcgctg atcgtccgcc agctcggttt cgaacccgtt cccggcgaga
     8461 tcggcgaagc gaaaggccgc catatcggtc agatcggccg gcgggcggaa ccgatcccgg
     8521 cgactgcgct tttcgagctg gtcgggcggg aactcggccg gg
//
LOCUS       DAOQFI010000004         8373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_2905_length_8373_cov_3.572854, whole genome shotgun sequence.
ACCESSION   DAOQFI010000004 DAOQFI010000000
VERSION     DAOQFI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2905_length_8373_cov_3.572854"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PLE93_00135"
     CDS             <1..1281
                     /locus_tag="PLE93_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH85481.1"
                     /translation="TGSPEVPARAIAKALDGIAFELSEEFPAWRAKDRDDAWQVDVAE
                     LRAPDIERDLELRDFTVGAVAVSLENGEAIDPLGGLGDLAAGTIRVCGPTSFSDDPLR
                     LMRAARLVAQFGWEIEEATAELARQSAPRAGDPAGERTLAELILLIGARDPLAGLAAM
                     DRIGLFDWLLPEIGDLHDVVQGPNHHLDVYGHTMEVVEGILRIESDLEAFTGERAPEV
                     SAYLSRPLADGATRGAALRLGALFHDCAKPQTRSEEGGFVSFRGHDRLGAEQIDSRFR
                     SLRASRKLSSYIADLTRHHLILGFLVNQMPLDKRQVFAYLDHTDPVSIDVTLLTVADR
                     LAARGTAEIAGQKMVAGHLELAREMVGHALDWDRHGPPAQFLPGNELAEALGIEPGPE
                     LGNLMKELAAARFAGEIEDATEAVEHARAFLEAG"
     gene            1301..1519
                     /gene="infA"
                     /locus_tag="PLE93_00140"
     CDS             1301..1519
                     /gene="infA"
                     /locus_tag="PLE93_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNH85482.1"
                     /translation="MAKEEKIEMEGEITEALPNTMFRVKLDNDHEVLGHISGKMRRHY
                     IRILPGDRVKIELSPYDLTRGRITYRYK"
     gene            1578..2432
                     /locus_tag="PLE93_00145"
     CDS             1578..2432
                     /locus_tag="PLE93_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85483.1"
                     /translation="MFQYSMLRSPLTTDGLRVARVFAVLSGLAALILIAFAPHAEAKS
                     TQRIELGARNINTLQPNCGRDFTRDCTVEGKVTAFQSLSARYPGRNFVVPFNGKLVAW
                     SISLSNPTRVEIDNNGAQLPSFNLIFGSPAQANIAILRQVEKRKKGGPRFKLVRKSPI
                     QILNPYFGTKVTFALDVPLNVYEGNVVALTIPTWAPALWKPKACNQTDFGILDPGRCD
                     QAEKAYTWRGSRVARGEPETCGLSNDGGPNEQLQKTAPQTHLDSVRRYGCYYGGNVLL
                     YSAIIVGR"
     gene            2573..3397
                     /locus_tag="PLE93_00150"
     CDS             2573..3397
                     /locus_tag="PLE93_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85484.1"
                     /translation="MSRPALRLGALVAATLGLFACLVLAGAFGDADGKSNRRVVLGAT
                     RDKVTPNCGTRSGARACIAEGKVTGFQVFQKGVTGKNFVVPFKKGKVVAWSIQLSNPL
                     KEDSRRFGPAQQPYFNKLFGSPSKARISILRPIKKGARYPPRYKLIRASGTQKLNRYF
                     GSEIKFAIKPLKVIRGDVVALTIPTWAPAFWVPMACSPSDRGTANAARCADARKANTW
                     RGSRGPGQCAIGTNAFDQPNEALRNSHPQQKVNSIKRYACYYDAGRLLYKATVVAP"
     gene            complement(3474..3746)
                     /locus_tag="PLE93_00155"
     CDS             complement(3474..3746)
                     /locus_tag="PLE93_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85485.1"
                     /translation="MSTESVSNEQIYRLRAAASGREAIAPVKDGVVYRDAETGEELEP
                     VAAVLPTTGEGSTLPRTPVNLRVCRRCEQLTPRDLDTCEFCGLPAS"
     gene            3768..5318
                     /locus_tag="PLE93_00160"
     CDS             3768..5318
                     /locus_tag="PLE93_00160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH85486.1"
                     /translation="MKILIFHGYLLRGTGSNIYNANLARALAGLGHEVHLLCQDREAG
                     SLDWVNAVGTWPDAEGPESIGELQFEQIRQPGGEGSVTVYRPPIGKLLPVYVEDPYEG
                     FEARAFPRLTDQEIDFYVGSNVAAVEAVERQIGGSDAALANHLIMGPVILARAGVRPY
                     AVKNHGSDLEYTIKPNPRFIPWAAEGVESAAAVLVGSHHTAASMWEALPGLGLEEKTG
                     FGPPGVDTQAFAPLKPGEHEGRIAALRNEIANEPPSTTFGRDADEAVAALDAFGAAEG
                     PRVIFVGKLIVSKGVDLIVAAWPLIHRQNPGARLMLVGFGAYEMGLRALVTALTDGDI
                     QSARDIAAIGRGLEGGEEEPLSYLTDFFSDLPAGYEEAGVQAAGSIAFAGRLDHDEVA
                     RVVPAADSMIVPSTFPEAFGMVAAEAAASGVLPVCAEHSGLAEVTATLAEEVPAVESM
                     IGLPLGPGAVAELAGKVNRWLALDPAERLEIGQNIAESADRNWSWQGVAKDVISASAG
                     QVRKVVQS"
     gene            5396..6187
                     /gene="coxB"
                     /locus_tag="PLE93_00165"
     CDS             5396..6187
                     /gene="coxB"
                     /locus_tag="PLE93_00165"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNH85487.1"
                     /translation="MVLAVIVLGVVQSVVLLQVDWFGDAASTAAGPIDTLLDVTIVVS
                     SFVFAIVCVALGYALIKWRAKPGDESDGLPIHGNTKLEIVWTIIPIIMVLGLAGYSWA
                     VLDDIEKTDADAIHINAYAQQFAWTFGYPESGNKWSEGELHVPVDRQIIIDLQAQDVL
                     HSFWVPEWRIKKDAVPGLETDAIITPDKEGTYQLICAELCGIGHTTMRAIVVVESEAK
                     YQAWLKQQTTEVPDDLLLTTDQALEKQKQQQEQSGGIEGSGVKEQ"
     gene            6226..8139
                     /locus_tag="PLE93_00170"
     CDS             6226..8139
                     /locus_tag="PLE93_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNH85488.1"
                     /translation="MAAAIRQPGWYRAAIGTVLGAAFGFFLVVGLRLLSGLDAFQTEQ
                     TGYPQVIVPLITTPFGFLFGFGAFDYWFRWAAGKPTIPEDHSGHGATRWQDYFTFNTD
                     HKVIGTQYVVTTLFFFLIGGLLALLMRAELAQPGGQIVAANTFNGLFSTHAVVMIFLF
                     IIPIFAGLANYVLPLMIGAPDMAFPRLNALSFWLLPIAGIIFLGSFLAPGGSFDAGWT
                     GYAPLSTGAPLGQSFFNIGVQFAGASSIATALNFLVTIITMRAPGMNLWRMPLLVWAN
                     LSTSLLVVGATPFVAGAQFMVFFDRILGMNFFNFLEGGDVISYQHIFWFYSHPAVYIM
                     MLPGFGIISEIISTHARKPVFGYRLMALALIGIVVLSYSVWAHHMFVAGMFSWLRVPI
                     MITTMLIAVPTGIKVFSWLGTLFYGKIHTYSTAMLFSLAFIITFTVGGISGVMTAMIP
                     LDIHVSDTYFIVAHIHFVLFGGSVFTIFAGIYHWFPKMTGRMYDERLGRIHFWGTLVG
                     TWMTFVPMHWIGMDGMPRRVADYATQYGNWNLLISVSAFVLGAVQLIFLYNMIVSWRF
                     GPKAGNNPWRANSIEWQVSSPPPVFNFDEIPRVVGGPYEYGTPGARHAIMGGEGEGAE
                     VPETQPSTVTAPS"
     gene            8136..>8373
                     /locus_tag="PLE93_00175"
     CDS             8136..>8373
                     /locus_tag="PLE93_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85489.1"
                     /translation="MSKPLLIFLNEVAGGRKLLEAAREKAADASYVVVVAPQNQPSVG
                     QLFDPEERAEAARARVDVTLAVLDEFGIEAIGEVL"
BASE COUNT         1446 a         2792 c         2608 g         1527 t
ORIGIN      
        1 accgggtcgc cggaggtccc ggcgcgggcc attgccaagg ctctggacgg aatcgccttc
       61 gagctttcgg aggagtttcc cgcctggcgg gccaaggatc gcgacgacgc ctggcaggtg
      121 gacgtggccg aactcagggc cccggacatc gagcgggatc tggaactgcg cgacttcacg
      181 gtcggcgcgg tggccgtaag cctggaaaac ggcgaggcga tcgacccgct cggcggactt
      241 ggcgatctcg ctgccggcac gatcagggtc tgcggcccga catcgttcag cgacgacccg
      301 ctgcggctga tgcgggcggc ccggctggtc gcccagttcg gctgggagat cgaggaggcg
      361 accgcagagc tggcacggca atccgccccc cgggccgggg acccggccgg tgagcgcacc
      421 ctggccgagt tgatcctcct gatcggggcc cgcgatccgc tggccgggct ggctgcgatg
      481 gaccggatcg gcctctttga ctggctgctg ccggaaatcg gtgacctcca cgatgtggtc
      541 cagggtccga accaccatct cgacgtctac ggccacacca tggaagtggt cgaagggatc
      601 ctcagaatcg aatccgacct cgaggctttc accggcgagc gggcacccga ggtttccgct
      661 tacctctccc ggccgctggc cgacggcgct acccgcggcg cggcgctccg gctcggggcg
      721 ctcttccacg attgcgccaa accgcaaacc cgaagcgagg agggcggctt cgtctccttc
      781 cgtggccacg accggctcgg ggccgaacag atcgacagcc ggtttcgcag cctgagggcc
      841 agccggaaac tgtcctccta cattgccgac ctgacccggc atcatctgat tctcgggttc
      901 ctggtcaacc agatgccgct cgacaaacgc caggtcttcg cctacctcga ccacaccgat
      961 ccggtctcga tcgatgtgac tttgctcacg gtggccgacc gcctggctgc ccggggcacg
     1021 gccgagatcg ccggccagaa gatggtcgcg ggtcacctcg aactggcccg ggagatggtc
     1081 ggccacgccc tcgactggga cagacatggc ccgccggcac agtttctgcc cggcaacgag
     1141 ctggccgagg ctctgggcat tgagccgggc ccggagctcg gaaatctcat gaaagaactg
     1201 gctgccgccc gttttgccgg tgaaatcgag gatgcaaccg aagctgtaga gcatgcccgt
     1261 gccttcctcg aagccgggtg agtagtagcc tcatctccgc atggctaaag aagaaaagat
     1321 cgaaatggag ggtgaaatca ccgaagccct cccgaatacg atgttcaggg tcaagctcga
     1381 caacgatcat gaggtccttg gtcacatctc gggcaagatg cgtcgccact acatccgcat
     1441 cctgccgggg gaccgggtca agatcgagct ttcgccctat gacctgaccc gcggccgcat
     1501 cacctaccgc tacaagtaac gatcgccacg accggggtcc gatcagatcc cggagcgcaa
     1561 cattgaggca cccaaccgtg ttccagtatt cgatgcttcg ctcccccctc acaactgacg
     1621 gccttcgggt cgcccgggtt ttcgcggtcc tgagcggcct tgccgccctg atcctgatcg
     1681 ccttcgcacc gcatgcggag gccaagtcca cgcagcggat cgaactcggc gcccgcaaca
     1741 tcaacaccct tcagccgaac tgcggccggg acttcactcg cgactgcacg gtcgaaggca
     1801 aggtgactgc cttccagtca ctttccgccc ggtatccggg gcgcaacttc gtggtgccgt
     1861 tcaacggcaa gctggtcgcc tggtcgattt cactttccaa cccgacccgg gtcgagatcg
     1921 acaacaacgg agcccagctt ccctccttca acctgatctt cggttcaccg gcacaggcca
     1981 atatcgcaat cctcaggcag gtcgagaagc gcaagaaggg tggcccgcgc ttcaagctgg
     2041 ttcgcaagag cccgatccag atcctcaatc cctacttcgg gaccaaggtc accttcgcgc
     2101 tcgacgttcc gctcaacgtc tacgaaggca acgtcgtcgc actcacgatc ccgacctggg
     2161 cgccggcgct ctggaagccg aaggcctgca accagaccga tttcggcatt ctcgaccccg
     2221 gtcgttgcga tcaggccgag aaggcctaca cctggcgtgg cagtcgcgtg gcccggggtg
     2281 aacccgaaac ctgcggactg agcaacgacg gcggcccgaa cgagcagctc cagaagaccg
     2341 ctccgcagac ccatctcgac tcggttcgcc gttacggctg ctactacggc ggcaacgtcc
     2401 tgctctactc ggcgatcatc gtcggccgct gaccccggtc ggatatttcc ccggctacga
     2461 gccgttggac cgtccgggca ggatttcccg accgccgctc tgacttgaag ttccgcacct
     2521 gtggcgtagc ctcggtgttg gatcctgtat ggccgaacct cgttcacatt tcgtgagccg
     2581 cccggccctt cgcctcggcg cgctcgttgc agccactctg ggtctcttcg cctgcctcgt
     2641 ccttgccggc gctttcggcg atgccgatgg caagagcaac cggcgggtcg tgctcggggc
     2701 aaccagggac aaggtcaccc cgaactgcgg taccaggtcc ggcgcacggg cctgcatcgc
     2761 ggaaggcaag gtgaccggtt tccaggtctt ccagaagggg gtcaccggca agaacttcgt
     2821 ggttcccttc aagaagggca aagtcgtggc ctggtcgatc cagctttcga atccgctcaa
     2881 ggaagacagc cgccggttcg ggcctgccca gcagccttac ttcaacaagc tctttggctc
     2941 accctcgaag gcacggatct cgatcctccg gccgatcaaa aagggcgcca ggtacccgcc
     3001 ccgctacaag ttgatcaggg ccagcggcac ccagaagctg aatcgctact tcggcagcga
     3061 gatcaagttc gcgatcaagc cgctcaaggt gatccgcggt gacgtcgtcg ccctcacgat
     3121 cccgacctgg gcgccggcct tctgggttcc gatggcatgc agtccttccg atcggggcac
     3181 tgcgaatgcg gcccgctgcg ccgacgcgcg caaggccaac acctggcggg gcagccgcgg
     3241 acccggccaa tgcgcgattg gcaccaatgc cttcgatcag ccgaatgaag ccctgaggaa
     3301 ttcgcatccc cagcagaagg tcaattcgat caagcgatac gcctgctatt acgacgccgg
     3361 ccgcctgctc tacaaggcga ctgtcgtcgc tccctaagac tgaggatccg ggtgcttgta
     3421 cttagttggt cagtggccaa ctaagtacaa gcacccggat gaaactcgtg aaatcaggaa
     3481 gcggggaggc cgcagaactc gcaggtgtcg agatcgcggg gggtgagctg ctcgcagcgg
     3541 cggcagaccc tgaggttgac cggagtgcgg gggagggtgg aaccttcgcc cgtggtcggc
     3601 agaacggccg cgaccggctc tagttcttcg ccggtttcgg cgtcccggta gacgacgccg
     3661 tccttgacgg gggcgatggc ctcgcgaccg gaagccgcgg cacgaagtcg gtagatctgt
     3721 tcgttggaga cgctttcggt gctcatcaag gtggagaata gccggacatg aagatcctca
     3781 tctttcacgg ctacctgctc cgcggcaccg gaagcaacat ctacaacgcc aacctggccc
     3841 gggctctcgc cggcctcggc cacgaagtcc acctgctctg tcaggaccgc gaggccggga
     3901 gcctcgactg ggtgaatgcg gtcggcacct ggccggacgc ggaagggccg gaatcgatcg
     3961 gcgagcttca gttcgaacag atccgccagc ccgggggcga ggggtcggtg acggtctacc
     4021 ggccgccgat cgggaaactg ctgcccgttt atgtcgagga tccgtacgag gggttcgagg
     4081 cccgggcctt cccccggctg accgatcagg agatcgactt ctacgtggga tcgaacgtgg
     4141 ccgccgtgga ggcggtggag cggcagatcg ggggatccga cgccgccctg gccaatcacc
     4201 tgatcatggg tccggtgatc ctcgcccggg ccggggtgcg gccatacgcg gtcaagaacc
     4261 acggatccga cctcgagtac acgatcaagc ccaacccccg attcatcccc tgggcggccg
     4321 aaggagtgga atccgcagcg gcggtcctgg tcggttcgca ccacacggcg gcctcgatgt
     4381 gggaggcgct gcccggtctc ggcctcgagg aaaagaccgg cttcggcccg cccggcgtcg
     4441 acacgcaggc gttcgctccg ctgaagccgg gcgagcatga gggccggatc gcggctctca
     4501 ggaacgagat cgcaaacgag ccgccgtcaa ccacgttcgg gcgggacgcc gacgaagcgg
     4561 ttgctgccct ggacgctttc ggggccgcgg aagggccgcg ggtgatcttc gtcggcaagc
     4621 tgatcgtctc caagggagtc gacctgatcg tcgccgcctg gccgctgatc caccgccaga
     4681 accccggggc gaggctgatg ctggtcggct tcggcgccta cgagatgggt ttgcgggccc
     4741 tggttaccgc gttgaccgac ggggacatcc agtcggcccg cgacatcgcc gcgatcggcc
     4801 gcgggctcga aggcggggag gaggaacccc tttcctacct gaccgacttc ttctcggacc
     4861 tgccggccgg atacgaggag gccggtgtcc aggcggccgg gtcaatcgcc ttcgcgggcc
     4921 ggctggatca cgatgaggtg gccagggttg tgccggccgc cgactcgatg atcgtgccct
     4981 cgaccttccc cgaggcattc ggcatggtcg ccgccgaagc ggccgcttcc ggggtgcttc
     5041 cggtctgcgc cgagcactcg ggcctggccg aggtcaccgc cacgctggcc gaggaagttc
     5101 ccgccgtgga atcgatgatc gggctgccac tcgggccggg agccgtggcc gagctggccg
     5161 gcaaggtcaa ccgctggctg gccctcgacc cggcggagcg gcttgagatc ggtcaaaaca
     5221 ttgccgagtc cgccgatcgg aattggagct ggcagggggt cgcaaaggat gtgatttccg
     5281 catcagcagg ccaagtgcgg aaggtcgtcc agtcctgagc gcagggtcag aaagcattcg
     5341 tccccggctc taatagaatg gcggtcgcct catgactcgt ctcccgattt tgaaaatggt
     5401 gctggccgta atcgtcctcg gcgtggtcca gtctgtggtt ctgctccagg tcgactggtt
     5461 cggcgatgca gcttccaccg cggccggtcc gatcgacacg ctgctggacg tcaccatcgt
     5521 cgtctcgtcg tttgtcttcg cgatcgtctg cgtcgccctc ggctacgccc tgatcaagtg
     5581 gcgggccaag cccggtgacg aaagcgacgg actgccgatc cacggcaaca cgaagctcga
     5641 gatcgtctgg acgatcatcc cgatcatcat ggtgctcggc ctggccggct actcgtgggc
     5701 cgttctcgac gacatcgaga agaccgatgc ggacgcgatc cacatcaacg cctatgccca
     5761 gcagttcgcc tggaccttcg gttacccgga gagcggcaac aaatggtcgg agggcgagct
     5821 tcacgttccg gtcgaccggc agatcatcat cgaccttcag gcgcaggacg ttctgcactc
     5881 cttctgggtt ccggaatggc ggatcaagaa ggacgcggtg ccgggacttg aaaccgacgc
     5941 gatcattacg ccggacaagg aaggcaccta ccagttgatc tgtgccgagc tctgcggcat
     6001 cggccacacg acgatgcgtg cgattgtggt tgtcgagtcc gaagcgaagt accaggcctg
     6061 gctcaagcag cagaccaccg aagtgcctga cgatcttctc ctgaccaccg atcaggcgct
     6121 ggagaagcag aaacagcagc aggagcagtc cgggggcatt gaaggaagcg gggtcaaaga
     6181 acagtgaagg cccggttcca caacccgatg atggaggtca agtaaatggc agcggcaatc
     6241 aggcaacccg gttggtatcg cgcagcgatc ggaaccgttc tcggtgccgc gttcgggttc
     6301 tttctggtgg tcggactgcg ccttctttcg ggccttgacg ccttccagac cgagcagacc
     6361 ggctacccgc aggtcatcgt cccgctgatc accacgccct tcggtttcct cttcggcttc
     6421 ggtgcctttg actactggtt ccgctgggcg gcaggcaagc cgaccatccc ggaagatcat
     6481 tccggccacg gcgccacccg ctggcaggac tacttcacct tcaacaccga ccacaaggta
     6541 atcggcaccc agtacgtggt gaccaccctg ttcttcttcc tgatcggtgg cctgctcgcc
     6601 ctgctgatgc gggccgaact ggcccagccg ggcggccaga tcgtcgccgc caacaccttc
     6661 aacggtctct tctccaccca cgcggtggtg atgatcttcc tcttcatcat tccgatcttc
     6721 gccgggctcg ccaactacgt gctgccgctg atgatcggcg ccccggacat ggccttcccg
     6781 cgcctcaacg cgctttcctt ctggctgctg ccgatcgccg gcatcatctt cctcggcagt
     6841 ttcctcgccc cgggcggttc cttcgacgcc ggctggaccg gttacgcgcc gctctcgacc
     6901 ggagccccgt taggccagtc cttcttcaac atcggcgtgc agttcgcggg tgcctcctcg
     6961 atcgccacgg cactcaactt cctggtcacg atcatcacca tgcgggcgcc tggcatgaac
     7021 ctctggcgca tgccgctgct ggtctgggcc aacctctcga cctcgctgct ggtcgtcggt
     7081 gcgaccccgt tcgtcgccgg tgcccagttc atggtcttct tcgaccgcat cctcgggatg
     7141 aacttcttca acttcctcga gggcggcgac gtcatctcgt accagcacat cttctggttc
     7201 tactcacacc cagccgtcta catcatgatg ttgcccggct tcgggatcat ttccgagatc
     7261 atttccaccc atgcccgaaa gccggtcttc ggctaccggc tcatggccct cgccctgatc
     7321 ggcatcgtcg tgctctccta ctcggtctgg gcgcaccaca tgttcgtcgc cggcatgttc
     7381 agctggctgc gagtgccgat catgatcacc acgatgttga tcgcggtacc gaccggcatc
     7441 aaggtcttct cctggctggg cacgctcttc tacgggaaga tccacaccta ttcgacggcg
     7501 atgctgttct cgctggcctt catcatcacc ttcaccgtcg gcggaatctc gggcgtgatg
     7561 accgccatga tcccgctcga catccacgtc tcggacacct acttcatcgt cgcccacatc
     7621 cattttgtgc tcttcggcgg ctcggtcttc accatcttcg ccggcatcta tcactggttc
     7681 ccgaagatga ccggacgcat gtatgacgag cgactcggcc ggatccactt ctggggcacc
     7741 ctggtcggta cctggatgac cttcgtcccg atgcactgga tcgggatgga tggcatgccc
     7801 cgccgtgtcg ccgattacgc gacgcagtac ggcaactgga acctgctgat ttccgtctcg
     7861 gccttcgtgc tcggtgcggt ccagctgatc ttcctctaca acatgatcgt cagctggcgg
     7921 ttcggcccga aggcgggcaa caacccgtgg cgcgcgaact cgatcgagtg gcaggtctcc
     7981 tctccgccgc cggtcttcaa cttcgacgag atcccgcggg tcgtcggcgg tccctacgag
     8041 tacggcacgc cgggtgcgcg tcacgcgatc atgggcggcg aaggcgaagg ggcggaagtg
     8101 cccgaaaccc agcccagcac ggtgactgcg ccgtcatgag caagcccctc ctgattttcc
     8161 tgaacgaggt cgccggcggc cgcaagctgc tcgaggcggc ccgcgaaaag gcggccgacg
     8221 cctcctacgt ggtggtcgtc gccccgcaga accagccttc ggtcggtcag ctcttcgatc
     8281 ccgaggaaag ggcggaagcc gcccgggccc gggttgacgt gaccctcgcg gtgctcgacg
     8341 agtttggtat cgaggcaatc ggcgaagtgc tcg
//
LOCUS       DAOQFI010000005         8129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_3043_length_8129_cov_3.115928, whole genome shotgun sequence.
ACCESSION   DAOQFI010000005 DAOQFI010000000
VERSION     DAOQFI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3043_length_8129_cov_3.115928"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLE93_00180"
     CDS             <1..839
                     /locus_tag="PLE93_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85490.1"
                     /translation="AEMREAMLGAGITNAIPAAGLTASNALGRESDLLGCATEDPQPG
                     CGVLVHFDVQVLRNQLPAVVFAQLLFGMELMKADPNWAGMNMVQPEDAAYSTSDYNLH
                     MRMVRYLRSVYPDESVTLHAGELVPGIVPPDDLRFHIRQAVNIAGADRIGHGADLRWE
                     RNPDQILRSMKSKGTCLEANLTSNQQILRLSGKRHPITDYVRAGIKVTLSTDDEGVSR
                     TDLTAQYVKAVRQHGFGYRRLKQFATYGLRCAFLTAAEKKAALADQKQRFRKFEARFP
                     SR"
     gene            complement(829..1590)
                     /locus_tag="PLE93_00185"
     CDS             complement(829..1590)
                     /locus_tag="PLE93_00185"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNH85491.1"
                     /translation="MSRLIDLSAPIEPGPEGAPEVVRIDVEYTPHEEGAKQIQALFGV
                     GPELLRDGEGWAVDTFTLLGTHSSTHVDAPWHYNSTIQGKPAETIDQLPLDWFFRPGF
                     LLDFHERADGEVVEIADIEAELRRTGHEIAERDICLVRTGCDQYLGTLEYMAHGPGVS
                     AAATRWLYDRGVRVMGIDAWGWDRPLWMQAEEAKEKGEAGIFWEAHQADIPYSQIERL
                     ANLDQLPATGFTVACFPLKLVGGSAAPARVVAIID"
     gene            1614..2636
                     /locus_tag="PLE93_00190"
     CDS             1614..2636
                     /locus_tag="PLE93_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HNH85492.1"
                     /translation="MTATDGAKGTVLVTGGSGFLGGRAIIDLLERGYDVRTTVRSKSK
                     APRIEENISNEIGHPAGIEFFEADLASDDGWAEAVSGCRYVLHIASPFPPNKPKDPQE
                     LIKPAREGAVRVINAALDAGVERIVMTSSVAAIRGGENPEDRFYNEDDWTDVDAVGAY
                     PQSKTLAEQAAWDLVDQRGERQKLATICPSAILGPVMPDDDSYSLEGIQRLLDGMPAV
                     PEIGFSWVDVRDVSAAHIAAMEKPEAGGQRFIASGPFLWLLEVSEILREKLPELAKKA
                     PTRKAPKFMVRIMAIFDPGARPLIADIGQRNDFDTTRAKEVLGIEARPLEETFVECAE
                     SLAARG"
     gene            complement(2889..3581)
                     /locus_tag="PLE93_00195"
     CDS             complement(2889..3581)
                     /locus_tag="PLE93_00195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH85493.1"
                     /translation="MTPKEAIPFSVDPIRIHLRFSPKGARHISIRVRKLGSGRTIRRI
                     VTGRLAGARWHHIYWDGLTQRRRLAPAGRYLVVAGPIGGPLKRVGRIRLHSHEFPVDG
                     PHGTRGYIGSFGAPRVDGRTHEGFDITAACGTPLVAVRAGRIIKKAYDPELYGNFIVL
                     KGKGERRTYKYAHLIRPAPVRRGQRVRAGRRLGSVGQTGNAAGTPCHLHLEIRTHGHL
                     LNPEPLVNAWDW"
     gene            complement(3738..4322)
                     /gene="clpP"
                     /locus_tag="PLE93_00200"
     CDS             complement(3738..4322)
                     /gene="clpP"
                     /locus_tag="PLE93_00200"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNH85494.1"
                     /translation="MSPLVPMVVEQTSRGERSFDIYSRLLNERIIFLGTGVNEDIANL
                     VVAQLLHLDAEDPEKDISLYINSPGGSVYAGLAIYDTMQFIKPDVQTICVGIAMSMGA
                     LLLAGGAAGKRMSLPNSKILIHQVSSGFQGQATDIEIHANEIIDTRSRLDKILAKHTG
                     QDLEKITHDTERDYFMSAEEATTYGLIDRVIEHR"
     gene            complement(4443..5819)
                     /gene="tig"
                     /locus_tag="PLE93_00205"
     CDS             complement(4443..5819)
                     /gene="tig"
                     /locus_tag="PLE93_00205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNH85495.1"
                     /translation="MKTTVTELEDSRARIEVEVPADEVSVQVQRAARALAREMRMPGF
                     RKGKAPPSLAIQRLGYDTVFQEAIQEGLPEWYQKSVYASGVVPIGSPDVEITKAPTQE
                     GETVEFKYEVGVRPNAELGEYKGLEVEKAGTEVPDEVIDREIDRMREGMASLDVVDRA
                     AEEGDHVLVDFVGSLDGVEFEGGSANDHTIEIGSGQLIDDFEEQIIGAKAGDEVAVNV
                     NFPEEYGAAELAGQNADFKVTVKEVRVKQMPEADDDFASEASEFDTIEELRADIAEKL
                     GESAGQRIEQDFRVAAVDAAVKNAKVGIPEDILRARGEERWERVERQMAQQGMNPDTY
                     LQMQGKTRDEIIDESLDDADMEIRREAVLVAVADAEGIEVTDEEMAEELEHAASHERT
                     TGAKLLERLKRDGRHEMVIADIKVRKAMDVIADSATATEMSSEETAKKLWGLGDDEED
                     SETEPSSE"
     gene            complement(5874..6446)
                     /locus_tag="PLE93_00210"
     CDS             complement(5874..6446)
                     /locus_tag="PLE93_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNH85496.1"
                     /translation="MSDEYLLIHFEDGEQLGAWLEENGETHDEVWVEIYKKHTGVKSI
                     AWAEIVDTVLCFGWIDSQSRRIDEDRYRQRLTPRRKRSKWSKINCDKAEALIADGRMR
                     PAGLAEVERAKEDGRWEEAYDSPNTATVPEEFEKALEAAGLTEKFSKLKSQERYSMLH
                     RIQTAKRADTKARLIEKFCGMISRGEQVHP"
     gene            complement(6443..6565)
                     /locus_tag="PLE93_00215"
     CDS             complement(6443..6565)
                     /locus_tag="PLE93_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85497.1"
                     /translation="MKRAPIRAWLVTGPPGRLVGFLLDFARALRAHRRAKKAER"
     gene            complement(6567..6968)
                     /locus_tag="PLE93_00220"
     CDS             complement(6567..6968)
                     /locus_tag="PLE93_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH85498.1"
                     /translation="MAGVYLDTSALGHLVLDEPDADVIGSALTRFEVVVSSRLLRTEL
                     HRLGLRTGVPSDEIASWLTSLALVPVDEEILSAAESIGPASVATLDAIHLVTAIRLAA
                     SGHISSVMTFDVRLAEGAAQHQLEVIAPVGE"
     gene            complement(6974..7258)
                     /locus_tag="PLE93_00225"
     CDS             complement(6974..7258)
                     /locus_tag="PLE93_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNH85499.1"
                     /translation="MPKVGVRELRQNLSRHLQRVKAGESLEVTEHGRVVARLSPAGEN
                     LPEAYLALAADHGAQVPSTSLSDLVANRPRRATPAGTVDAMLAEGRAERS"
     gene            complement(7391..>8129)
                     /locus_tag="PLE93_00230"
     CDS             complement(7391..>8129)
                     /locus_tag="PLE93_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNH85500.1"
                     /translation="ETDDGERRLELNEGQAIHSIYRPGEYLVGNYWDKHLVLPFVAPP
                     GVTGARQATWATLPAHPKPLKPWPRRIAILGNAAGTVARAYGHFYPGTKIDAVEIDGK
                     VTEVGRKYFGLDNPNMEVHTEDARPWLEAADPVYDVIMVDAYHQPYIPFYMATKEFFE
                     LVRDRLRPGGVVLINVGHPENSDALEENLTATLEEALPEVLRDPVTDTNTMLVGAAGP
                     IEPGNIIQATKGKSSPIPPARFGSARL"
BASE COUNT         1291 a         2680 c         2657 g         1501 t
ORIGIN      
        1 tcgccgagat gcgcgaggcg atgctgggtg ccgggatcac caacgcgatt ccggccgccg
       61 ggcttaccgc atccaacgcg ctcggtcgcg aatccgacct gctcggctgc gcgaccgagg
      121 acccgcaacc cggctgcggc gtgctggtcc acttcgacgt ccaggtcctg cgcaaccagc
      181 tgccggcggt ggtcttcgcc cagcttctct tcggaatgga acttatgaag gccgatccga
      241 actgggccgg catgaacatg gtccagcccg aggacgcggc ctactcgact tcggattaca
      301 acctccacat gcggatggtc aggtacctga ggtcggtcta tccggatgag agcgtgaccc
      361 tccacgccgg cgagctggtg ccgggcatcg tgccacccga cgacctccgc ttccacatca
      421 ggcaggcggt gaacatcgcc ggtgccgacc ggatcggcca cggcgccgac ctgcgctggg
      481 agcgtaaccc cgaccagatc ctccgcagca tgaagagcaa ggggacctgc ctggaagcga
      541 atctgacctc gaaccagcag atcctccggc tgtccggaaa gcgccacccg atcaccgatt
      601 atgtgcgggc cggaatcaag gtgaccctct cgaccgacga cgaaggcgtg tcccgcaccg
      661 acctgaccgc gcagtacgtg aaagcggtgc ggcagcacgg cttcggttac cggcggctga
      721 agcagttcgc gacctacggc ctgcgctgcg ccttcctcac cgcggccgag aagaaagccg
      781 ccctggccga tcagaagcag cgcttccgga agttcgaagc gcgcttccct agtcgatgat
      841 cgccacgacc cgggcggggg cggcgctgcc gccgaccagc ttgagcggga agcaggccac
      901 ggtgaaaccg gtcgccggca gctgatcgag attggccagc cgctcgatct gggaataggg
      961 aatgtcggcc tgatgggcct cccagaagat cccggcctcc cccttctcct tcgcctcttc
     1021 ggcctgcatc cagagcggcc ggtcccagcc ccaggcgtcg atccccatca cccggacgcc
     1081 gcgatcgtag agccagcgcg tggcggccgc gctcacaccg ggcccgtggg ccatgtattc
     1141 gagggtgccg aggtactggt cacagccggt ccggaccagg cagatgtccc gttccgcaat
     1201 ttcatgtccg gtccgccgaa gttccgcctc gatgtcggcg atttcaacca cctcgccgtc
     1261 ggcccgctcg tggaagtcca gcaggaaccc gggccgaaag aaccagtcga gcggaagctg
     1321 gtcgatcgtc tcggccggct tgccctggat cgtcgagttg taatgccacg gagcgtcgac
     1381 gtgggtcgag ctgtgggtgc cgagcagggt gaaggtgtca accgcccagc cttcgccgtc
     1441 gcggagcagt tcggggccga ccccgaacag cgcctggatc tgcttcgcac cttcctcatg
     1501 cggcgtgtat tcgacgtcaa tgcggacgac ttcgggggca ccttcgggtc ccggctcgat
     1561 cggagccgat agatcgatca atctgctcat gcaaggcaga ctagagtgcg gtcatgaccg
     1621 caactgatgg cgcgaagggg acggtcctgg tcaccggagg ctccggcttc ctcggtggcc
     1681 gggcaatcat cgatctgctc gagcggggct acgacgtgcg gaccacggtc cgcagcaagt
     1741 cgaaggcgcc gcgaatcgag gagaacatct cgaatgagat cggccacccg gccgggatcg
     1801 agttcttcga ggctgacctc gcctccgacg acggctgggc cgaagcggtt tccggttgcc
     1861 ggtacgtgct ccacatcgcc tcgccgttcc cgcccaacaa accgaaggat ccgcaggagc
     1921 tgatcaagcc cgcccgtgag ggtgcggttc gggtgatcaa tgccgccctc gacgccgggg
     1981 ttgagcgcat cgtgatgact tcctcggttg ccgcgatccg tggcggcgag aaccccgaag
     2041 accgcttcta caacgaggac gactggaccg atgtggatgc ggtcggcgcc tacccgcagt
     2101 cgaagacgct ggccgagcaa gccgcctggg acctggtcga tcagcgcggc gagcgccaga
     2161 agctggcgac gatctgcccc agcgcgatcc tcggcccggt catgcccgat gacgattcgt
     2221 attccctcga gggaatccag cgcctgctcg atggtatgcc ggcggtgccg gagatcggct
     2281 tcagctgggt cgacgtgcgc gacgtttccg ccgctcacat cgccgcgatg gagaagccgg
     2341 aagccggcgg gcagcgtttc atcgcttccg ggccgttcct ctggctgctc gaggtctcgg
     2401 agatcctgcg ggagaagctg cccgagctgg cgaagaaggc cccgacgcgc aaggccccca
     2461 agttcatggt ccggatcatg gcgatcttcg atccgggcgc ccgcccgctg atcgccgata
     2521 tcggccagcg caacgacttc gacaccaccc gggccaaaga ggtgctggga atcgaggccc
     2581 ggccgctcga agagaccttc gtcgagtgcg ccgaatccct cgccgcccgg ggttagtggt
     2641 tacgccgcgg ggccgggtga gcatcgccgg gacggaccac gcgcggcatt cagccgcgct
     2701 ggccctggag tgtcccggac gacgctcacc cggccccacg gctacacgaa cccggttcgt
     2761 gattagaggt gccgcacgtt caggaccttg gtggtgctgt ttgtacggca cctccacgga
     2821 ctacttggct ccaggacccg cgagtttcca ctttgcgcca cataccggca caaagtgggc
     2881 attcgtcctt accaatccca ggcgttcacg agaggctcgg ggttgagaag gtggccgtgg
     2941 gtgcggattt ccaggtggag gtggcagggg gttccggcgg cgtttccggt ctggccgact
     3001 gagccgaggc ggcggccggc cctcaccctc tggccgcggc ggaccggggc cggccggatc
     3061 aggtgggcgt acttgtaggt gcgcctctcc cccttgccct tcagcacgat gaagttgccg
     3121 tagagctcgg ggtcatatgc cttcttgatg atcctgcccg cccgcaccgc gaccagcggg
     3181 gtaccgcagg cggcggtaat gtcaaagccc tcgtgggtgc ggccgtccac ccggggtgcg
     3241 ccgaagctgc cgatgtagcc gcgggtgccg tgcggcccgt cgaccgggaa ctcgtgcgag
     3301 tgcaggcgga tccgcccgac ccgcttcagg ggcccaccga tcggcccggc aaccaccagg
     3361 taacggccgg cgggggcgag ccggcgccgc tgggtgaggc cgtcccaata gatgtgatgc
     3421 cagcgcgcac cggcgagccg accggtgacg atgcgacgga ttgtccggcc ggacccgagc
     3481 ttcctgaccc tgatcgaaat gtgacgggct cccttcgggc tgaagcgaag atgaatccgg
     3541 atcgggtcaa ccgagaaggg gattgcttct ttcggcgtca cctcaccggc caggagccgg
     3601 aatccgcttc ccccttcgcc cgccccggcg tgggaaagtt ccgacgtggc ggatgcgtgc
     3661 cctgcaaaga ccagagccgc cgccaggatg gcgacggctc cggtgaaact cttgaggctc
     3721 cctttcagga tgcgcctcta ccgatgctcg attacccggt cgatcaggcc gtaggtggtg
     3781 gcctcttcgg cgctcatgaa gtagtcgcgc tcggtgtcgt gggtgatctt ctcgaggtcc
     3841 tggccggtgt gcttggcgag gatcttgtcg agccggctgc gggtgtcgat gatctcgttg
     3901 gcgtggattt cgatgtccgt ggcctgaccc tggaaaccgg aggagacctg gtggatcagg
     3961 atcttcgagt tgggcagcga catccgcttg cccgcggcgc caccggcgag cagcagggcg
     4021 cccatgctca tcgcgatgcc gacgcagatc gtctggacgt cgggcttgat gaactgcatc
     4081 gtgtcgtaga tcgcgaggcc cgcgtaaacg gagccgccgg gcgagttgat gtagaggctg
     4141 atgtccttct cgggatcctc agcgtccagg tggagcagct gggccaccac caggttcgcg
     4201 atgtcctcgt tcacgcctgt gccgaggaag atgatgcgct cgttgaggag ccgggaatag
     4261 atgtcgaacg aacgctctcc gcgagaggtc tgctcgacga ccatgggaac aagtggactc
     4321 atgccgccat cctatagagg gtagttatcg ggccgactcc accagagccg gcccatccgt
     4381 caaatgtctc tgtgggccgg cgggtttctc gtggagggcg ggtcggaccc cgcccgactt
     4441 gcttattccg aagagggttc ggtttccgaa tcctcttcgt cgtccccgag gccccagagc
     4501 tttttggccg tctcttcgga ggacatttcg gtggccgtcg ctgaatcggc gatcacatcc
     4561 atggccttgc ggaccttgat gtcggcgatc accatttcgt ggcggccgtc gcgcttcagt
     4621 cgttcgagca gcttggcgcc ggtggtgcgc tcgtgggacg cggcgtgctc gagttcctcg
     4681 gccatctcct cgtcggtgac ttcgatccct tcggcatccg ccactgcgac caggacagcc
     4741 tcgcggcgga tctccatgtc ggcgtcgtcg agcgactcgt cgatgatctc gtcccgggtc
     4801 ttgccctgca tctggagata ggtgtcgggg ttcatgccct gctgggccat ctgccgctca
     4861 acccgctccc agcgttcttc gccccgggcc cggaggatgt cctcgggaat cccgaccttg
     4921 gcgttcttca ccgcggcgtc gaccgcggcg acccggaagt cctgctcgat ccgctgcccg
     4981 gcggattcac ccagcttctc ggcgatgtcg gcgcggagct cctcgatcgt gtcgaactcg
     5041 gaagcctcgg aggcgaagtc gtcatcggcc tcgggcatct gcttcacccg gacttccttg
     5101 acggtcacct tgaagtcggc gttctggccg gccagctcgg ccgccccgta ctcctcgggg
     5161 aagttcacgt tgacggcgac ctcgtccccg gccttggcgc caatgatctg ctcttcgaag
     5221 tcgtcgatca gctggccgga gccgatctcg atcgtgtggt cgttggcgga gccgccctcg
     5281 aactcgaccc cgtcgagcga gccgacgaaa tcgacgagga cgtggtcgcc ctcctcggcc
     5341 gcacggtcga ccacgtcgag gctggccatg ccctcgcgca tgcggtcgat ctcgcggtcg
     5401 atgacctcgt ccggcacctc ggtcccggcc ttctcgactt cgagaccctt gtattcaccg
     5461 agttcggcgt tcgggcgaac gccgacctcg tacttgaact cgaccgtctc gccttcctgg
     5521 gtcggagcct tggtgatctc gacgtccggc gagccgatcg gaaccactcc cgacgcgtaa
     5581 acgctcttct ggtaccactc gggcaggccc tcctggatcg cctcctggaa gacggtgtcg
     5641 tagccgagtc gctggatcgc cagcgagggc ggcgccttgc ccttgcggaa accgggcatg
     5701 cgcatctcgc gggccagcgc ccgggcggcc cgctggacct ggacggacac ttcgtcggcc
     5761 ggaacttcga cctcgattcg tgccctcgaa tcttctagtt cagtaacagt ggtcttcaag
     5821 gtcgaacgag ggtatcgggg accggctttc cgccgcttcc agagccgccg cgcctaggga
     5881 tggacttgct cgccgcgact gatcatgccg cagaactttt cgatcaggcg ggccttggtg
     5941 tctgcccgtt tcgcggtctg gatccggtgg agcattgagt agcgctcctg ggatttcagc
     6001 ttcgagaact tctcggtcag gccggcggct tccagcgcct tctcgaactc ctcggggacg
     6061 gttgcagtgt tcgggctgtc gtaggcctcc tcccacctgc cgtcttcctt tgcccgttcc
     6121 acctcggcca gaccggccgg acgcatcctc ccgtcggcga tcagcgcttc cgccttgtcg
     6181 cagttgatct tcgaccactt gctgcgtttg cgccgcgggg ttagccgctg gcggtaccgg
     6241 tcctcgtcga tccggcgact ctgactgtcg atccagccga aacagagcac cgtgtccacg
     6301 atctcggccc aggcgatcga cttcaccccg gtgtgcttct tgtagatctc gacccagacc
     6361 tcgtcatggg tctcgccgtt ttcctccagc caggcgccca gctgttcgcc gtcctcgaag
     6421 tggatcagga ggtactcgtc gctcatcgtt cggccttctt cgcccgccgg tgggcgcgca
     6481 gggccctggc gaagtcgagg aggaagccga ccaggcggcc gggtgggccg gtgaccagcc
     6541 aggctcggat gggggcgcgc ttcatctcac tcaccgaccg gggcgatgac ttcgagttga
     6601 tgttgtgccg cgccttcggc gagtcgaaca tcgaatgtca tcaccgacga gatgtgacct
     6661 gaggctgcga gccggatcgc ggtgacgagg tggatggcgt cgagcgtcgc gacagacgcg
     6721 ggcccgatcg actcggcggc tgagaggatc tcttcgtcga ccggcaccag ggcgaggctg
     6781 gtcaaccacg atgcgatctc atcacttggt acgccagttc gaaggccgag gcgatgaagc
     6841 tcggtacgaa ggagacggct tgagacgaca acctcaaacc gggtcaaagc cgaaccgatc
     6901 acatcggcat cgggctcgtc aagcaccaaa tggccgagcg cgctggtgtc cagataaacg
     6961 cccgccatcg gtttcaggat cgctcggcgc gaccctcggc cagcattgcg tcaaccgtgc
     7021 cagccggggt cgctcgcctt gggcgattcg cgaccaggtc cgacaaactg gtacttggca
     7081 cctgggctcc atggtctgcg gccagtgcca gataagcctc aggcaaattt tcacccgccg
     7141 gggaaagcct ggctaccacg cgcccgtgct cggtgacttc gagggactca cccgccttca
     7201 cgcgctgaag gtggcgactc aggttctgtc tcagctctcg aactcctact ttcggcattg
     7261 tgcgtcacac cgtagcacat tgactgaatc caccacaaaa gaccttctca ctcgcgggcg
     7321 tagtccagca gggaaaggtc gaccagccat tcgaccgggg cgtggtcatc ggtccagatg
     7381 tctcccccgc tcagaggcgg gcggatccga atcgcgccgg cgggataggt gagctcttgc
     7441 ccttggtggc ctggatgatg ttgcctggct cgatcgggcc ggccgcgccg accagcatcg
     7501 tgttggtgtc agtgaccgga tcgcggagga cctcgggcag agcctcctcg agggtggcgg
     7561 tcaggttctc ttccagggcg tcgctgtttt cgggatggcc gacgttgatt aggaccaccc
     7621 cgcccgggcg gagccggtcc cggaccagtt cgaagaattc cttcgtcgcc atgtagaagg
     7681 gaatgtaggg ctggtggtag gcatcgacca tgatcacgtc atagaccggg tcagccgcct
     7741 cgagccacgg tcgggcatcc tcggtgtgaa cctccatgtt cggattgtcg agaccgaagt
     7801 acttccgccc cacttcggtc accttgccgt cgatctcgac cgcgtcgatc ttcgtcccgg
     7861 gatagaagtg accgtaggcc cgggcgaccg tgccggcggc gttgccgagg atggcgatcc
     7921 gccgtggcca tggctttaac ggcttcgggt gagccggcag agtcgcccac gttgcttgcc
     7981 gggcgccggt cacccccggc ggcgccacga agggcaggac caggtgcttg tcccagtagt
     8041 tgccgaccag gtactcgccc ggccggtaga tcgagtggat cgcctgcccc tcgttcagct
     8101 ccagccgccg ctcgccgtca tcggtttcg
//
LOCUS       DAOQFI010000006         7913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_3181_length_7913_cov_2.998218, whole genome shotgun sequence.
ACCESSION   DAOQFI010000006 DAOQFI010000000
VERSION     DAOQFI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3181_length_7913_cov_2.998218"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLE93_00235"
     CDS             <1..461
                     /locus_tag="PLE93_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85501.1"
                     /translation="FNKSYFMGVRDVDEPYALLVAALRDSGKAGIGRFTFHNREYLVA
                     LHAEEDRLVLHTLRYAEEIIEPGDLDLPDGGKKPTRKEVELARRLVEGLTEDFDPGEY
                     QDEYRASVMDLIERKAEGKKPKKKRARKREETGDLSKALEKSLAAQGSRS"
     gene            461..1378
                     /locus_tag="PLE93_00240"
     CDS             461..1378
                     /locus_tag="PLE93_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNH85502.1"
                     /translation="MPRPLWKGTISFGLVSVPVEMWSAIRDRNIRFREIHVEDGSPVR
                     HVRYCAEEGKPVDPSEVGRAYELDDGRLVRITDEELEGLEPEKTQTIEIESFAPRAQV
                     EPLRFDRPYNLIPGNQTVGTIRAYRLLVEAMAESDLLALGKFVMRGREYLAAIRVREN
                     VLTLSTMHFPAEIRSTEDVPGPAKKADKEAVKDAVAVIEERSVEWDPGKYPNEHRERL
                     MAVIRKKDKGRKIKAPAPVAEERPTGAPDLMAALKKTLAESRSKRRKPRDLARLSKEE
                     LYEMASERGLKGRSSMNKQQLLKALRANR"
     gene            complement(1383..1976)
                     /locus_tag="PLE93_00245"
     CDS             complement(1383..1976)
                     /locus_tag="PLE93_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNH85503.1"
                     /translation="MKALTINCTLKASPATSNTEALATVVADEIQQAGVETDLVRAVD
                     HNILPGVSNDEGEGDGWPAVLDRVRAAQILIIATPTWLGQPSSVAKRVLERMDALISE
                     TDDEGVPFAYGRVAGVVVTGNEDGAHHVISEISGSLIDIGYTIPGQAWTYWNRGPGPG
                     DDYLSNEAGHDWSHETGRTAARNLLTAARSLEGAWSG"
     gene            2108..2668
                     /locus_tag="PLE93_00250"
     CDS             2108..2668
                     /locus_tag="PLE93_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNH85504.1"
                     /translation="MSQEQMNQKLKDKRVAILVANEGVEEIELKGPLQALRDAGAEVD
                     LVAPEEGQVQAFNHLDKGSPFTVDRTVSEVSSDEYAALVLPGGVANPDNLRTHEDAVA
                     FAGSFFEAGKPVGAICHAPWTLVEAGVLEGRTLTLWPSLKTDIENAGATWVDEEVHVD
                     GEFVTSRRPDDLESFCHQVVETIAAA"
     gene            complement(2683..3153)
                     /locus_tag="PLE93_00255"
     CDS             complement(2683..3153)
                     /locus_tag="PLE93_00255"
                     /inference="COORDINATES: protein motif:HMM:NF039409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HNH85505.1"
                     /translation="MGSSPRDEENWQRLDRNLDELLQELRIALPGVQVLFAFLLAVSF
                     QQRFGGVTDFQQSVYFATLMLTGASTFLLIAPTAFHRVTFRQQQKERLVRLANRLAIA
                     GLGCLGLAVIGAVTLVTDFLYGAAPTALAAGAFLVIATVLWVVIPVRVRTRHRD"
     gene            3273..3539
                     /locus_tag="PLE93_00260"
     CDS             3273..3539
                     /locus_tag="PLE93_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNH85506.1"
                     /translation="MPKGSDHGPSVKDDKLYEELREDGASKEKAARIANAKAGGENVS
                     KKGGESEDYEDRTVEELQDRAAEIGIEGRSDMNKKELISALRNH"
     gene            3566..4009
                     /locus_tag="PLE93_00265"
     CDS             3566..4009
                     /locus_tag="PLE93_00265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNH85507.1"
                     /translation="MIVFLVVDMISRWDFDGADRAADRAGDAVEAINRLRRRAGENLK
                     VVYANDMDGGFHGSRETAFALAMSGRHPELVSPLEPRPDEDFIHKGQHSAFYGTPLAH
                     LLDVCDVETIWLAGQATEQCILYTALDAHVRGSGYYRSQSTPYWR"
     gene            4015..4494
                     /locus_tag="PLE93_00270"
     CDS             4015..4494
                     /locus_tag="PLE93_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85508.1"
                     /translation="MAEISTVEEKLAEVTGLAQAAQGAIEKVAPMIDDESVSESLEKM
                     KQEAVETEERCTSAIEKRDGRKTAILDKARETKQEATEMMSTYLGDDSDGLDGLEFMT
                     MAEAGEVGHWEILGELSQRAGESELEELTGWALPIQERHLAEVRKGSLTLASQEDIE"
     gene            4499..5131
                     /locus_tag="PLE93_00275"
     CDS             4499..5131
                     /locus_tag="PLE93_00275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNH85509.1"
                     /translation="MAEALIIDIDGTLVDSNYQHVIAWQEALREHGLDAEAWKIHRYV
                     GKGGDKMIESVAGEEAERKAGDAVRESEGEIFQRLIDQVRPLEGAGEFVRKVKDLGFI
                     VVLSSSAKDHEVDHYLDQLEVRELIDGHTSSADVDQTKPDPDLIEAALEKAGTRDALM
                     VGDSIWDVEAARRSGIDCLAVLTGGFSKAELAAAGAIEVRPSLADLEIPD"
     gene            5173..6714
                     /locus_tag="PLE93_00280"
     CDS             5173..6714
                     /locus_tag="PLE93_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH85510.1"
                     /translation="MPLAGLVDEGQESPWLESGSEPFAPVSEDIRCDVAVVGGGIVGV
                     STAYRLARAGCSVVLVEARRLAAGVTGHSSAKLSALQGLAYSAIADRNGGEAASAYAE
                     LNARGIRFVEETTAALGIDCGLTHRPAVSYAETDEGRENLALEMDAALAAGLAVTDED
                     LAPLPFPVAATLTLENQAHFDPVKWVRGLAGALTELGGRVHETSRVTGVDGLRGNRKV
                     KMENGVTIEAERVVLATHVPILDRAAFYARVKPLTSFAVAGQIEGPLPQGLYLSVDGP
                     TRSISPLPGGGGTRSLLVGGESHRPGTEDSAVSVARLRQFMRSRFEVRSVDCAWGAHD
                     MMSFDRLPIVGRLLPFDSRILTATGFSKWGLAAGAGSSELLADQLLGEDNPVAATFDP
                     VRLRLSSVPTLVRHNLESGAMLLADRINRRRPRRRLQPGEGMVVGDGVGQKAVSCDRD
                     GVYRELSARCTHLGCIVAWNQAGQSWDCPCHGSRFSPDGSVIEGPAVAPLERSQVITP
                     PAGDS"
     gene            complement(6711..>7913)
                     /locus_tag="PLE93_00285"
     CDS             complement(6711..>7913)
                     /locus_tag="PLE93_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNH85511.1"
                     /translation="FHYTLARPEGAGPGTKGLSLFVVPKWHFDSETGELGTRNGVNVT
                     SIEHKMGIKASATCDMAFGDGDNPAVGYLVGDVHQGIRQMFDVIEFARMMVGTKAIAT
                     LSTGYLNALEYAKERVQGADLTQIMDKTAPRVTVIAHPDVRRSLMTQKTYAEGLRALY
                     LFTALHHDPVAAEVSQGVDAEFAAKINDFLLPIVKGVGSERAYEVLTESLQTYGGSGF
                     LQDYPIEQYIRDAKIDSLYEGTTAIQAQDFFFRKIVRDEAKSLNHLLEKMDATAAGSG
                     AGLELERERLTAAIASMREIVGSMMGYLMGSQEDPKSIYKVGLGSVQFLKAFGDLLIG
                     WLLISQAEIAAAALENGAAGRDRDFYEGKITGARFFARNMLPNLEATKLAIAQIDNDV
                     MELDEAAF"
BASE COUNT         1527 a         2463 c         2680 g         1243 t
ORIGIN      
        1 acttcaacaa gtcctacttc atgggtgtgc gggatgtgga cgagccttac gcgctgcttg
       61 tcgcggcact ccgggacagc ggcaaggcag ggatcggccg gtttaccttc cacaaccgcg
      121 agtacctggt cgcccttcac gccgaggagg acaggctggt gctccacaca ctgcggtacg
      181 ccgaggagat catcgagccg ggtgacctcg acctgccgga cggcggcaag aagccgaccc
      241 gcaaggaagt cgagctggcg cgccggctgg tcgagggact gaccgaggac ttcgaccccg
      301 gcgagtacca ggacgagtac cgcgcgtccg tgatggatct gatcgagcgg aaggccgagg
      361 gcaagaagcc gaagaagaag cgggcccgca agcgtgagga gaccggggac ctttccaagg
      421 cgctcgaaaa gagcctcgcc gcgcagggga gtcgttcctg atgccgcgac cactctggaa
      481 ggggacgatt tcgttcggcc tggtcagcgt gccggtcgag atgtggtcgg cgatccggga
      541 ccgcaacatc aggttccgtg aaatccacgt ggaagacggt tcgccggtca ggcacgtgcg
      601 ctactgcgcc gaagaaggca aaccggtcga tccctcggaa gtcggtcgtg cctacgaact
      661 cgatgacggc cgcctggtcc ggatcaccga cgaggaactg gagggactcg aaccggagaa
      721 gacacagacc atcgagatcg aatccttcgc cccgcgcgcc caggtcgaac cgctccgctt
      781 tgaccggccg tacaacctga ttcccggcaa ccagaccgtg ggcacgatcc gtgcgtaccg
      841 tctcctggtc gaggcgatgg cagaaagcga cctgcttgcg ttgggcaagt tcgtgatgcg
      901 gggacgggag tacctggccg cgatccgggt ccgggaaaat gttctcacgc tctcgacgat
      961 gcatttcccg gccgagattc gatctacgga ggacgtgccg ggaccggcga agaaagcgga
     1021 caaggaggcg gtcaaggacg ccgtcgcggt gatcgaggaa cgctccgtcg aatgggatcc
     1081 cggcaagtat ccgaacgaac atcgtgagcg gctgatggca gtgatcagga agaaggacaa
     1141 gggccggaag atcaaggctc ccgctcccgt cgccgaagag cggccgaccg gtgccccgga
     1201 tctgatggcc gccctgaaga agaccctggc ggaatcccgc agcaagcgca ggaagccgcg
     1261 cgacctcgcc cggctctcaa aggaggagct ttacgagatg gcctccgaac gaggcctcaa
     1321 gggccgctcc tcgatgaaca agcagcagct gctcaaggcg ctccgcgcca accgctagaa
     1381 ggtcagcccg accaggctcc ttcgagggaa cgggcggcgg tgagcaggtt cctggccgca
     1441 gtccttccgg tctcgtggga ccagtcgtgc ccggcctcat tcgaaaggta gtcatcaccc
     1501 ggtccgggac cccggttcca gtaggtccat gcctgaccgg gaatcgtgta gccgatgtcg
     1561 atcaggctgc cggagatctc gctgatcaca tgatgcgcgc cgtcttcatt cccggtcacc
     1621 accacgccgg cgacccggcc gtaggcaaac ggaacgcctt catcgtcggt ctcgctgatc
     1681 agcgcgtcca ttcgttccag cacccgcttg gcgacgctcg aaggctgacc gagccaggtc
     1741 ggcgtcgcga tgatcaggat ctgcgccgcg cggacccggt cgagcactgc aggccagccg
     1801 tcaccttcgc cttcatcgtt gctgactccg ggaaggatgt tgtggtccac cgcccggacc
     1861 aggtcggtct cgaccccggc ctgctggatt tcgtcggcga ccacggttgc cagggcttcg
     1921 gtgtttgagg tggccggact ggccttcagg gtgcagttga tggtcaacgc tttcattccc
     1981 ggccggtacc cctgccggga aggccaaaac gtgccgccac cagaatcagg gcgggtccgg
     2041 acgcggatcc agggttgacc gggggccgaa cgggtaaccg gtaattgaac ccacaagagg
     2101 agaaacgatg agccaggaac agatgaatca gaagctgaag gacaagcgtg tggcgatcct
     2161 cgtggccaac gagggcgtcg aggaaatcga actgaagggg ccgctgcagg cgctgaggga
     2221 cgccggagcg gaagttgacc tggtcgcacc cgaggagggt caggtccagg ccttcaatca
     2281 cctcgacaag ggaagtccct tcaccgtgga ccggaccgtt tccgaggttt cgagtgacga
     2341 gtacgcagcc ctcgtcctcc cgggtggcgt ggcaaacccg gacaacctga gaacccacga
     2401 agacgcggtc gcctttgccg gttccttctt cgaggcgggc aagccggtcg gagcgatctg
     2461 ccatgccccc tggaccctgg tcgaggccgg cgtcctggaa ggccgaacgc tgaccttgtg
     2521 gccaagcctg aagacggaca tcgaaaacgc cggcgctacc tgggtcgacg aggaggtcca
     2581 tgtcgacggc gagttcgtca ccagccggcg cccggacgac ctcgaatcgt tctgtcacca
     2641 ggtcgtagag acgatcgccg ccgcctgagc tccgtccgcg ggtcagtccc ggtgccgggt
     2701 gcgaacccgg accgggatga ccacccacaa cacggtcgcg atcaccagga aggctcctgc
     2761 cgcgagggcc gtgggggccg cgccgtagag aaagtcggta accagcgtca ccgcgccgat
     2821 cacggccaga ccgaggcagc cgagccctgc aatggccagg cgattggcaa gtctgacgag
     2881 gcgttccttc tgctgctggc gaaatgtcac gcggtgaaac gcggtcgggg cgatcaacag
     2941 aaaggtcgag gcccccgtca gcatcagggt cgcgaaatag accgactgct gaaaatcggt
     3001 cacaccgccg aaacgctgct ggaacgaaac cgccagcaga aacgcgaaga ggacctggac
     3061 tccgggcagg gcgatcctga gttcctggag caactcgtcc aggttccggt caaggcgttg
     3121 ccaattctcc tcatcgcgcg ggctcgatcc catacccccg aggtacccgg acggcctcga
     3181 ctcgaaccgg actggttttg acgcgcaacc gtcgggtacc agaaacggta gccaaggttg
     3241 actggcaaat tcgaacgcaa aggagactcg aaatgccgaa aggatcagac cacggaccga
     3301 gcgtcaagga cgacaagctc tacgaagaac tccgcgaaga cggcgccagc aaggaaaaag
     3361 cggcccggat cgccaacgcg aaggcgggcg gagagaacgt aagcaagaaa ggcggcgaat
     3421 ccgaggatta cgaggatcgc accgtcgagg aactgcagga ccgggccgcc gagatcggca
     3481 tagaaggccg ctccgacatg aacaagaagg aactgatcag cgccctgcgc aatcactgac
     3541 ccctgacggc cggacggcca gcgcgatgat cgtattcctc gtcgtcgaca tgatctctcg
     3601 ctgggatttc gacggtgcgg accgggcggc ggatcgggcc ggtgacgcag tcgaagcgat
     3661 caaccggctg cgccgccgtg ccggggagaa cctgaaggtc gtttacgcaa acgacatgga
     3721 cgggggcttc cacggatccc gggaaacagc ttttgccctg gcgatgtcgg gccggcaccc
     3781 ggaactggtt tcacccctcg aaccccgtcc cgatgaggac ttcatccaca agggtcagca
     3841 ctcggccttc tacggcacgc cgctggcaca cctgctcgac gtctgcgacg tggaaacgat
     3901 ctggctggcc ggccaggcga ccgagcagtg catcctctac acggcactgg acgcccacgt
     3961 caggggcagc gggtactacc gcagccagtc aaccccttac tggaggtaat cgaaatggca
     4021 gaaatatcga cggtcgagga gaagctcgcc gaggtcactg gcctggccca ggcagcccag
     4081 ggagcgatcg agaaggtcgc accgatgatc gacgacgaat cggtgtcgga gagcctggag
     4141 aagatgaagc aggaagcggt cgagaccgag gagcggtgca ccagcgcgat cgagaagcgg
     4201 gacggtcgca agaccgcgat tctcgacaag gcccgcgaaa ccaagcagga agcgaccgag
     4261 atgatgtcga cctacctcgg tgacgactcg gacgggctcg acggactcga gttcatgacc
     4321 atggccgagg ccggcgaggt gggccactgg gagatcctgg gtgaactcag tcagcgggcc
     4381 ggagaatccg aacttgagga actcacggga tgggcgctgc cgatccagga gcgtcacctg
     4441 gccgaagtaa ggaagggctc gctgaccctt gccagccagg aagacatcga gtgacttcat
     4501 ggcagaagct ctgatcatcg acatcgacgg caccctggtc gactccaact accagcacgt
     4561 gatcgcctgg caggaagccc tgagagagca cgggctcgac gccgaagcgt ggaagatcca
     4621 tcgctacgtg ggcaagggtg gcgacaagat gatcgaatcc gttgccggcg aggaagccga
     4681 gcggaaagcc ggcgacgcgg tacgcgaaag tgaaggcgag atcttccagc ggctgatcga
     4741 tcaggtcagg ccgctggaag gcgccggcga attcgtacga aaggtgaagg acctcggctt
     4801 catcgtcgtg ctttcgagtt cggccaagga ccacgaggtc gaccattacc tcgaccagct
     4861 cgaggtcagg gaattgatcg acgggcacac cagttccgcc gacgtggacc agaccaagcc
     4921 cgatccggac ctgatcgaag cggccctgga gaaggcgggg acccgtgacg ccctgatggt
     4981 tggcgattcg atctgggacg tggaggcggc ccggcgctcg gggatcgact gcctcgccgt
     5041 cttgacgggc ggcttctcga aggcggaact cgcggcggcc ggggcaatcg aggtaaggcc
     5101 gtccctggcc gatctggaaa tcccggactg accaacgccg tggcgcccga aggcacagga
     5161 agtaaaaagc cggtgccgct cgcgggcctg gtggacgagg gccaggaatc gccctggctg
     5221 gaaagcgggt cggagccgtt tgccccggtc agcgaagaca tccgctgcga cgtggcggtg
     5281 gtgggtggcg gaatcgtcgg ggtctcgacg gcttaccggc tcgcccgggc gggatgctcg
     5341 gtggtcctgg tcgaggcgcg acggctcgcc gccggtgtga ccggtcactc gtcggccaag
     5401 ctgagcgcgc ttcagggtct ggcctactcg gcaatcgctg acaggaacgg gggcgaagcg
     5461 gcctccgcct atgccgaact gaacgcccgg ggcatccgct tcgtcgagga gacgaccgcg
     5521 gcactgggaa tcgactgcgg gctcacccac cggcccgcgg tttcgtacgc cgaaaccgat
     5581 gaaggacggg agaacctggc cctggagatg gacgcggcgc tggccgccgg cctcgcggtg
     5641 accgacgagg atctggcgcc cttgccgttt ccggttgccg cgacgctcac cctcgaaaac
     5701 caggcccact tcgacccggt caaatgggtc aggggtctcg ccggcgccct gaccgagctc
     5761 ggcggccggg ttcatgaaac aagccgggtg accggggtcg acggattgcg gggcaaccgc
     5821 aaggtcaaaa tggaaaacgg cgtgacgatc gaggccgaac gggtggtgct tgccacacac
     5881 gtaccgattc tcgaccgcgc cgccttctac gcccgggtca agcccctgac ctccttcgcg
     5941 gtcgccggcc agatcgaggg gccgctcccg cagggtctct acctgagtgt cgacggcccg
     6001 acccgctcga tcagccccct gcccggcggg ggaggtacgc gctcgttgct ggtcggcgga
     6061 gagagccatc gtccgggcac cgaggacagt gccgtgagcg ttgcccgcct gcgccagttc
     6121 atgaggtcaa ggttcgaggt ccgctccgtg gactgtgcct ggggcgccca cgacatgatg
     6181 agcttcgacc gcctgccgat cgtcgggcgg ctgctgccct tcgactcgag aatcctcacc
     6241 gcgaccggat tctccaaatg gggtctcgcc gcgggcgcag gcagttccga gctgctcgca
     6301 gatcaattgc tgggggaaga caacccggtc gcggcaacct tcgaccccgt ccgtttacgg
     6361 ctgagttcgg tgccgaccct ggtccggcac aatctcgaat ccggagccat gctgctggcc
     6421 gaccggatca accgccgccg gccccggcgc cgcctgcagc cgggcgaggg catggtggtt
     6481 ggcgacggcg tcggccagaa agcggtgagt tgcgaccgcg acggcgtcta ccgggaactc
     6541 tcggcccgct gcacccatct cggctgcatc gtggcctgga accaggcggg gcagtcctgg
     6601 gactgcccct gccacggttc gcgcttttca ccggacggga gcgtgatcga gggtccggcc
     6661 gttgctccgc tcgagcgaag ccaggtcatc acgcccccgg ccggggactc ctagaaggcg
     6721 gcttcgtcga gctccatgac gtcgttgtcg atctgggcga tcgccagctt ggtcgcttcc
     6781 aggttcggca gcatgttgcg ggcgaagaac ctggcgccgg tgatcttgcc ttcgtagaag
     6841 tccctgtcgc gcccggcggc accgttctcg agggcggctg cggcgatctc ggcctggctg
     6901 atcagcagcc agccgatcag caggtcgccg aaggccttga ggaactggac cgaaccgaga
     6961 ccgaccttgt agatgctctt cggatcttcc tgtgagccca tcaggtaacc catcatcgaa
     7021 cccacgatct cgcgcatgct ggcgatcgcg gcagtcaggc gttccctttc gagctcgagc
     7081 ccggcgccgg aaccggcggc ggtggcatcc atcttctcga gcaggtggtt gagcgacttc
     7141 gcctcgtcac ggacgatctt gcggaagaag aagtcctgcg cctggatcgc tgtagtgccc
     7201 tcgtagaggc tgtcgatctt ggcgtcacgg atgtactgct cgatcgggta gtcctgcagg
     7261 aagcccgaac cgccgtaggt ctggagcgat tcggtcagca cttcgtaggc acgctctgaa
     7321 ccgacgccct tgacgatcgg cagcaggaag tcgttgatct tcgccgcgaa ctctgcgtca
     7381 accccctgcg agacctcggc cgcgaccggg tcgtggtgga gagcggtgaa gaggtaaagc
     7441 gcccggaggc cctcggcgta ggtcttctgg gtcatcagtg accggcggac gtcgggatgg
     7501 gcgatgacgg ttacccgcgg ggcggtcttg tccatgatct gggtcagatc ggccccctgg
     7561 acccgttcct tcgcgtactc gagcgcgttg aggtaaccgg tcgaaagggt ggcgatcgcc
     7621 ttggtgccga ccatcatgcg ggcgaactcg atcacgtcga acatctggcg aatgccctgg
     7681 tggacgtcac cgaccaggta gccgacggcc gggttgtcgc cgtccccgaa ggccatgtcg
     7741 caggtggccg aagccttgat ccccatcttg tgttcgatcg aggtgacgtt gacgccgttg
     7801 cgggtgccga gctcaccggt ttccgaatcg aaatgccact tcggcacgac gaagaggctg
     7861 aggcccttgg tgccggggcc ggctccttcg gggcgggcca gtgtgtagtg gaa
//
LOCUS       DAOQFI010000007         7762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_3302_length_7762_cov_2.802517, whole genome shotgun sequence.
ACCESSION   DAOQFI010000007 DAOQFI010000000
VERSION     DAOQFI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3302_length_7762_cov_2.802517"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLE93_00290"
     CDS             <1..221
                     /locus_tag="PLE93_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85512.1"
                     /translation="HAAGPERGRRSKWRPVEVLRTNRRGLFRYRYRFRTITSAQRILF
                     RASALPEAGWPYLPSTTKPKSVIVYPKD"
     gene            273..674
                     /locus_tag="PLE93_00295"
     CDS             273..674
                     /locus_tag="PLE93_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85513.1"
                     /translation="MVVALIALTIAIGGTAAALPGKFTVGRDDLKTSSVGARALGKIV
                     ISHSQVFPSADPTAQDGNFTETIGTITCPDDAPTAIDPFVGGMSTTAYVFGRTAIFNR
                     FRAPQGYRYTVFSDDGPEVGYVLKVNCIPAR"
     gene            complement(818..1438)
                     /locus_tag="PLE93_00300"
     CDS             complement(818..1438)
                     /locus_tag="PLE93_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85514.1"
                     /translation="MAEAKKTTKKSTAKKTAAKKPAAKKRTAAQLRAEAEKKAIHAAK
                     LDAENAKSRLEEVQETAVEYAKKGVDVPVGAALNLADRVSEVIEDWSDQASAEKKVKG
                     YRADLTKTVKRAERRGITTRRKATTQARKQRKSLEKTVRQQRKSIEKTVKTTNKDVNK
                     QIEARVNEVTKRANQVQADVTKVADEQGKKAQDLVNKVTEQLSALV"
     gene            complement(1611..2450)
                     /locus_tag="PLE93_00305"
     CDS             complement(1611..2450)
                     /locus_tag="PLE93_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNH85515.1"
                     /translation="MNQLPPDPALDADWTGICRHIVEEQKKLFAEYETIQQRDNYEGV
                     GEGGDHALVLDRMCEDVIFAELEKLADSGGAAFTAISEERGTVLFNEGGNVWVVIDPI
                     DGSLNFKRTIPQHSLSIAVATAPNMGAVEFGYVYDFGTGDEFLAQSGEGAYLNEERIN
                     LEPREGLEVVGIEGADPTALAPMLAELAGKVYRVRCVGSLAISIASVAAGRFDALAFP
                     HLSRSVDVAAAQLIAREAGALVDLGDGGLSEVGLALEDRFPVTVAATGEGLETLVSIR
                     TVG"
     gene            2504..5563
                     /locus_tag="PLE93_00310"
     CDS             2504..5563
                     /locus_tag="PLE93_00310"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNH85516.1"
                     /translation="MSDSSSRTGADGDPGPGRELVRRHGSGALLIRGGAGTGKSTALR
                     ERVIHLARPETGRPGLDPCRVALIASTDRTAAAHRIRLEDDLPGPYEALSVFTWEGLA
                     EEILRHRPVEAGLGPSFEVVGAAERLAMLLARVDELPLRHQEIRGNPAGLLRELLIGI
                     DRAKREGTDGGELAELIEIHNRILAAADLLDRNELPRIATDLLADPALAAEVTARFPH
                     LVIDELEDLHPARAGLLQRLAVAGPESVVAGIDPSRAIYGPGAEERFLAAFPDLEQFT
                     LDEAWRMSEVRITAARAALGPGPLPEGAWEARGDEGSVRYWRPASARAEAQAAAREVE
                     HQIALGTPPEQLAIAVPDVNSQGVGVVGALAERGIPARPGGGAALFRQPEVRDTIAWL
                     RALTDPTDARAVTRALMRPPTELRSLDLARLTQISKRRKLDMISACEASLESPQMAPE
                     ARQRIEGFLNLYRAASGALDTRRPDAYVRRLIERIGFRRQRLFAARPETAERLLGLSR
                     LAQIATDFSRRDPNASVREFTVFLEALADGGLEPVGGPDLPGVGAVPVMNLDLLKGGE
                     WARIWVLGLERPFGITRNPGPGIGDDQTHPGGPETARVAVATALTSAREEVVLSRASE
                     AEPDPVAAILEATGGLEEIQGEELFGPAEDVAATYRAMLGEVMEASWKAGSELNEPRL
                     DTAIDVNRAVARYLELLKLAALAQRDDGSSDEEAIEAVNGLIAQVATPEQLAELDSST
                     LDPYLLAQERERGMRQGLADSRSEPSLDAFLPRRGDDLRLSATDLDMYLTCPLKYKFA
                     RVFGIPTPPTVNMRFGIVIHNVLQRFHELEQDVGRPVPDGEARLMALLEEGWRRGGFG
                     ETHDELQFLDRAREAMRAYWRSEEASESEPAFLERQFEFKVGPHFLRGRVDRVDRKPG
                     GEFEVIDYKTGQRIDSSRHGGDIQLALYRLGAREAWGIEPEAGSYYYVLEGEKVEVQA
                     SPDDRERVERTVLEVGEGVLGQDFEPRPSPGVCGWCDFRLVCPAAEA"
     gene            complement(5576..6439)
                     /locus_tag="PLE93_00315"
     CDS             complement(5576..6439)
                     /locus_tag="PLE93_00315"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HNH85517.1"
                     /translation="MDQLRWEMNPPKLRDPMMVCAFRGWNDAASAASTALETLADSLE
                     VDVLAEVDPEDFYDFQANRPTIRLEEGAHRRIEWPGNTFIAARAQGAERDLVLLDGTE
                     PNLKWRTYSEMVVQVAEQLDVQLVVVLGSLIAEVAHTLPVPITGVSTDEQTVRSLELE
                     RSDYEGPTGIVGVIHDRCRAAGLPSVSLWAAVPHYVAAVPNPKASLALSEKLESITRV
                     AADISGLEDETMNYEEQIGRAVAADPEVEELVERIEKEQRQRRGPELDVPSGDALAME
                     FQRFLKNRDSD"
     gene            6541..7428
                     /locus_tag="PLE93_00320"
     CDS             6541..7428
                     /locus_tag="PLE93_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH85518.1"
                     /translation="MEAAAKNGTPVVELTPGQSFTGRYACARKDRLTAKNGSAYLALE
                     LRDRTGSIPARIFRDSDRIGINFERGDVVAVKGRVEQFRGQLSAEIEDAKKIDPSEID
                     PADFLPTAYRDREELEGFLEHLAREVHHPGYRAVVEDLLFTGETAEAFRLAPCTRAGH
                     HAYIGGLSEHTVAVGTLVTETCVLHPKLNSDLLMAAALIHDIGKTGEFTYGAEIGLSE
                     RGRNLGHLQIGAELVGEAADRAGLDQAERDALLSTVMCHHGTDPLRLRQFPSAEAIAL
                     YRLNALDAQVKTALEHGLT"
     gene            complement(7463..>7762)
                     /locus_tag="PLE93_00325"
     CDS             complement(7463..>7762)
                     /locus_tag="PLE93_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNH85519.1"
                     /translation="GFLIRATGGGEVVVSCYGAIDRIELGAGEQIVVDSGHLVAFEPS
                     VQYKTRKASSGLMNTLKSGEGFVMEFTGPGVIHTQTRNPDFLVSWLTEVLPFTRA"
BASE COUNT         1251 a         2643 c         2592 g         1276 t
ORIGIN      
        1 cgcacgcggc tgggccggaa cgtgggcgac gaagcaagtg gcgaccggtc gaggtgctcc
       61 ggaccaaccg ccgggggctc ttccggtacc ggtaccgctt ccggactatc acctcggccc
      121 agcgaatcct gttcagggcc agcgccctgc ctgaggctgg ctggccctat ctgccctcga
      181 caacgaagcc gaaatcggtc attgtgtacc ccaaggactg atttggcagg gaaggaggca
      241 ggatgagagg gatacggaga ccgtcgccgg caatggtggt ggcactgatc gccttgacga
      301 tcgcgatcgg tggaaccgcc gctgccctgc ccggcaagtt cacagtgggc cgggatgatc
      361 tcaagacctc cagtgtcgga gccagggcgt tgggaaagat cgtaattagc cattctcagg
      421 tcttcccctc agcggatccg accgcccaag acggcaattt caccgagacc atcggaacga
      481 tcacttgccc cgacgatgcg ccaactgcga tcgatccttt tgttggcggg atgagcacta
      541 cggcctatgt ctttggcaga accgcaatat tcaaccggtt ccgtgccccc cagggctacc
      601 ggtacaccgt cttcagcgac gacggacccg aagttgggta cgtgttgaaa gtcaactgca
      661 tccctgcccg ttgatcacat cacttaagga ccaacggccc cgaggagggg ccgttggcga
      721 gggaggagag gtactacatc gactgacgag gttatgaggt cagccgatct tcaccggttc
      781 gccgttctcc tgcggcggcc gggctaagcg gtttggtcta gaccagagcc gaaagctgct
      841 ccgtgacctt gttgaccaga tcctgggcct tcttgccctg ctcgtcggcg accttggtga
      901 cgtcagcctg aacctggttg gcgcgcttgg tgacttcgtt cacgcgggct tcgatctgct
      961 tgttgacgtc cttgttggtc gtcttgacgg tcttctcgat gctcttccgc tgctggcgga
     1021 cggtcttctc gagcgacttg cgctgcttgc gggcctgggt ggtcgccttg cgacgggtgg
     1081 tgatgccacg acgctcggcg cgcttgacgg tcttggtcag gtcggcgcgg tagcccttga
     1141 ccttcttctc ggcgctggcc tggtcggacc agtcctcgat cacttcggaa acgcgatcgg
     1201 ccaggttgag ggcggcgcct accggcacgt caacaccctt cttggcgtac tcgacggcgg
     1261 tctcctggac ctcttcgagg cggctcttgg cgttctcggc gtcgagcttg gcggcgtgga
     1321 tggccttctt ctcggcctcg gcgcggagct gggcggcggt gcgcttcttg gcagccggct
     1381 tcttcgcggc ggtcttcttg gcggtgctct tcttggtggt cttcttggct tcggccatgg
     1441 ggatcagtta gtcctttgtt tgttgagatc agattactta cttctaagga tgtttgaagt
     1501 gtagcaacca aacacgaaca catgttcttt tttcgtgtga cctcggtcac gcagggagaa
     1561 tccgcatggc agagcggaaa acagcgccgg aatcggacgc aatccgggat tcagccgacg
     1621 gtgcgaatcg aaacgagggt ctccagaccc tcgccggtgg cggccacggt gaccgggaaa
     1681 cggtcctcga gggcgaggcc gacctcgctc agaccgccgt ccccgaggtc aaccagggcg
     1741 ccggcctcgc gggcgatcaa ctgggccgcc gccacgtcaa ccgagcggga gagatggggg
     1801 aaggcgagcg cgtcgaaacg gcccgcggca accgaagcga tcgagatcgc caacgagccg
     1861 acacagcgca cccggtagac cttaccggcc agttcagcca gcatcggggc gagtgcggtg
     1921 gggtcggccc cctcgatgcc gaccacctcg agcccctccc ggggctccag gttgatccgc
     1981 tcctcgttca ggtaggcccc ctcgccggac tgggcgagga actcgtcccc ggtcccgaag
     2041 tcatagacat agccgaactc gaccgccccc atgttcgggg cggtggcgac cgcaatcgaa
     2101 aggctgtgct gggggatggt ccgcttgaag ttgagcgaac cgtcgatcgg gtcgatcacg
     2161 acccacacgt tgccgccctc gttgaacagg acggtcccgc gctcctcgga tatcgccgtg
     2221 aaggcagcgc cgccgctatc ggcgagcttc tccagctcgg cgaagatcac gtcctcgcac
     2281 atccggtcga ggaccagggc atggtcgcct ccctcgccga cgccttcgta gttgtcgcgt
     2341 tgctggatcg tctcgtactc ggcgaagagc ttcttctgtt cctcgacgat gtggcggcag
     2401 atccccgtcc agtcagcgtc gagtgcgggg tccgggggaa gttggttcac ccgcgacatg
     2461 ctagacgctg tgaaacagga cgaggctcgg gtaatttgag ggggtgagcg attcctcgtc
     2521 cagaaccggt gccgacggag atcccggccc cggccgggaa ctggttcgca ggcacggctc
     2581 cggggcgctc ctgattcggg gcggcgcggg taccggcaag agcaccgccc tccgggaacg
     2641 ggtgatccac ctcgcgcggc cggaaaccgg ccggccgggc ctcgatccgt gccgggtcgc
     2701 cctgatcgcc tcgaccgacc gcaccgccgc cgcccaccgg atccggctcg aagatgattt
     2761 gcccgggccc tatgaagcgc tttcggtctt cacctgggag ggcctggccg aagagatcct
     2821 ccgccaccgg ccggtcgagg cagggctcgg accctccttc gaggtggtcg gcgcggccga
     2881 acgcctggcc atgctgctgg cccgggtcga cgagctgccg ctccgccacc aggagatccg
     2941 gggcaacccg gccgggctgc tccgggagct tctgatcggc atcgaccggg ccaaacggga
     3001 gggcaccgac ggcggcgagc tggccgaact gatcgagatc cacaaccgga tcctcgccgc
     3061 ggccgacctg ctcgaccgca acgaactgcc gcgcatcgcc accgacctgc tggccgatcc
     3121 ggccctcgcc gccgaggtca cggcccgctt cccccacctg gtgatcgacg agctcgagga
     3181 ccttcacccg gcccgggccg ggctgcttca gcgactggcc gtggccgggc ccgaaagcgt
     3241 ggtcgcgggc atagacccct cccgggcgat ctacgggccg ggcgccgagg aacggttcct
     3301 ggccgccttc ccggacctgg agcagttcac cctggatgag gcctggcgga tgagcgaggt
     3361 gcggatcacc gccgcccggg ccgcacttgg tccgggcccc ctgcccgaag gagcctggga
     3421 agctcgcggc gatgaaggct cggtccgcta ctggcggccc gcctcggccc gggcggaggc
     3481 acaggccgcg gccagggagg tcgaacacca gatcgccctg ggaactccgc cggagcagct
     3541 ggcgatcgcg gtgccggacg tgaactcgca gggagtcggg gtggtcgggg cgctggccga
     3601 acggggcatc cccgcccggc ccggcggtgg cgccgcgctc tttcggcagc cggaggtgcg
     3661 cgacacaatc gcctggctga gggcactgac cgacccgacc gacgcccggg cggtcacccg
     3721 cgccctcatg cggccgccaa ccgaactccg ctccctggac ctggcccggc tcacccagat
     3781 ctcgaaacga cgcaagctcg acatgatcag cgcctgcgag gcctcgctcg aatcaccgca
     3841 gatggcgccg gaggcccggc aacggatcga aggcttcctc aacctctacc gggcagcctc
     3901 gggcgcgctc gacacccgcc ggcccgacgc ttatgtgagg cggctgatcg agcggatcgg
     3961 ttttcgccgc cagcgactct tcgccgcgcg gcccgaaacc gccgaacgcc tgctcggcct
     4021 ctcccggctg gcccagatcg cgaccgactt ctcccgccgg gacccgaacg cctcggtgcg
     4081 ggagttcacc gtcttcctgg aagcgctggc cgacggcggc ctcgaaccgg tcggtgggcc
     4141 ggatctgccc ggagtcggag cggtgccggt gatgaacctc gacctgctca agggcggcga
     4201 gtgggcccgg atctgggtgc tcggtctgga gcgtccgttc gggatcaccc gcaaccccgg
     4261 cccgggcatt ggcgatgacc agacccaccc cggcggcccc gaaacggcgc gtgtcgcggt
     4321 cgccaccgca ctcacctcgg cccgggaaga agtggttctc tcgcgggcca gcgaggccga
     4381 accggacccg gtcgcggcga tcctcgaagc gaccggcggc ctcgaggaaa tccagggcga
     4441 agagctcttc ggaccggccg aggacgtcgc ggcgacctac cgggcgatgc tcggcgaggt
     4501 gatggaggcc tcctggaagg ccggctccga gctgaacgaa ccacggctcg acacggcgat
     4561 cgacgtgaac cgcgcggtcg cccgctacct cgagctcctg aagctggccg ccctggccca
     4621 gcgcgatgac ggatccagtg acgaggaggc gatcgaagcg gtcaacggcc tgatcgccca
     4681 ggtcgccacc ccggagcagc tggccgaact cgactcctcc accctcgacc cctatctgct
     4741 cgcccaggaa cgcgagcggg gcatgcggca gggcctcgcc gactcccgct cggaaccatc
     4801 gctcgatgct ttcctgcccc ggcgcggtga cgacctccgg ctctcggcga ccgacctcga
     4861 catgtatctg acctgcccgc tgaagtacaa gtttgcgcgg gtcttcggca tccccacacc
     4921 gccaaccgtg aacatgcgct tcgggatcgt gatccacaac gtcctgcagc gcttccatga
     4981 actggaacag gacgtcggcc ggccggtccc ggatggcgag gcccggttga tggccctgct
     5041 cgaggaaggc tggcgtcgcg gtggtttcgg cgagacccac gacgagcttc agttcctcga
     5101 ccgggcccgc gaggcgatgc gggcctactg gaggagcgaa gaggcctccg aatccgagcc
     5161 ggccttcctc gaacggcagt tcgagttcaa ggtcgggccg catttccttc gtggccgggt
     5221 cgaccgggtc gaccgcaagc ccggtggcga gttcgaggtg atcgattaca agaccggcca
     5281 gcggatcgac tcctcccgcc acggcggtga catccagctc gccctctacc ggctcggggc
     5341 gcgcgaggcc tgggggatcg aaccggaggc cggcagctac tactacgtgc tcgaaggcga
     5401 gaaggtcgag gtccaggcct caccggatga ccgcgagcgg gtcgagcgga ccgtgctcga
     5461 ggtcggcgaa ggggtgctcg gccaggactt cgaaccgcgc ccctcgcccg gggtctgcgg
     5521 ctggtgtgac ttcaggctgg tctgcccggc cgccgaagcc tgagcccggc aaaggtcagt
     5581 ctgaatcgcg gttcttgagg aaccgctgga actccatggc aagggcatcg ccgctgggga
     5641 cgtcgagctc cggcccgcgc cgctggcgct gttccttctc gatccgctcg accagttcct
     5701 cgacctccgg gtcggcggca accgcccgcc cgatctgctc ctcgtaattc atagtttcgt
     5761 cttcgagacc ggagatgtcg gcggcgaccc gggtgatcga ttcgagcttc tcgctgagcg
     5821 cgagcgacgc cttcgggttg gggaccgccg caacgtaatg gggcaccgcc gcccagagcg
     5881 aaaccgaggg caggccggcg gcccggcagc ggtcgtggat cacgccgacg atgccggtcg
     5941 ggccttcgta atcggagcgt tcgagctcga ggctgcggac ggtctgctcg tcggttgaaa
     6001 cccccgttat cgggaccggc agggtgtggg caacctcggc gatcagtgag ccgagcacga
     6061 ccaccagctg gacgtcgagc tgctcggcga cctggaccac catctcggag taggtccgcc
     6121 acttcaggtt cggttcggtc ccgtcgagca ggaccaggtc ccgttcggca ccctgggcgc
     6181 gggcggcgat gaaggtgttg cccggccatt cgattctgcg gtgggctcct tcttccaggc
     6241 ggatggtcgg ccggttcgcc tgaaagtcgt agaaatcctc cgggtccacc tcggccagca
     6301 cgtccacctc gagagaatcg gccagggtct ccagggcggt cgaggcggca ctcgccgcgt
     6361 cattccagcc gcgaaaggcg cagaccatca tcgggtcacg gagctttggc gggttcatct
     6421 cccaacggag ttggtccatt agttcaattt agccgacccg ctctcccggc cctgccgcct
     6481 gcgaccggtg gcagctcggc ggcgtcccgc ccgtctgcgg cttcggtcag gatgtggggg
     6541 atggaggccg cggccaaaaa cggaaccccg gtggtcgaat tgacccccgg ccagagcttc
     6601 accggccgct acgcctgcgc ccgcaaggac cggctcaccg caaagaatgg atcggcctac
     6661 ctcgccttgg aactgcgaga ccggaccggc tcgattcccg cccggatctt ccgcgactcc
     6721 gaccgcatcg ggatcaactt cgaacgtggt gacgtggtcg cggtgaaggg ccgggtcgag
     6781 cagttccgcg ggcagctctc ggccgaaatc gaggacgcga aaaagatcga cccgtcggag
     6841 atcgacccgg ccgactttct gccaaccgcc taccgcgacc gggaagaact cgaaggcttt
     6901 ctcgaacacc tcgcccggga agtccatcac cccggctacc gggcggtggt cgaggacctg
     6961 ttgttcaccg gcgaaaccgc cgaagccttc cggctcgccc cctgcacccg ggccggccac
     7021 cacgcctaca tcggcgggct ttcagagcac accgtcgcgg tcggcaccct ggtcaccgaa
     7081 acctgcgtgc tccatccgaa actcaactcc gacctgctga tggccgcggc cctcatccac
     7141 gacatcggca agaccggcga gttcacctac ggggccgaga tcggcctgag cgagcgcgga
     7201 cgcaacctcg gccacctcca gatcggggcg gagctggtcg gtgaagcagc cgaccgggcc
     7261 ggtctcgatc aggccgagcg ggacgccctg ctttcgaccg tgatgtgtca ccacggcacc
     7321 gatccgctgc ggttgcgcca gttcccctcg gccgaggcga tcgccctcta ccggctgaac
     7381 gcgctcgacg cccaggtcaa gaccgcactc gaacacggcc tgacctgagc gaaaagcggg
     7441 aacctccagc ggccggtcgc ggctaggccc gggtgaaggg caggacttcg gtcagccagc
     7501 tgaccaggaa gtccgggttc ctggtctggg tgtggatgac gccgggaccg gtgaactcca
     7561 tgacgaaacc ctcgccgctc ttcagggtgt tcatcaggcc cgaggatgcc ttgcgcgtct
     7621 tgtactgaac gctcggctcg aaggcgacca gatgaccgga gtcgaccacg atctgctcgc
     7681 ccgcgccgag ttcgatccgg tcgatcgcgc cgtagcagga gacgaccact tcgccaccgc
     7741 cggtcgcgcg gatcaggaag cc
//
LOCUS       DAOQFI010000008         7386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_3572_length_7386_cov_2.957714, whole genome shotgun sequence.
ACCESSION   DAOQFI010000008 DAOQFI010000000
VERSION     DAOQFI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3572_length_7386_cov_2.957714"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLE93_00330"
     CDS             complement(<1..427)
                     /locus_tag="PLE93_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85520.1"
                     /translation="MSVFLTIERAEAPAGHEPSRVPLVDADGQITIDPWWGTIQPQRL
                     HPGIPTWGELEVIAHIEAGGRLIDTRLPEYASASGTIPGSLVLPWETITDHLEVFDPA
                     SVTVLFCNGPQCAATPRAVGRLLDAGVEPESLAYYRGGIQ"
     gene            complement(526..1077)
                     /gene="cysC"
                     /locus_tag="PLE93_00335"
     CDS             complement(526..1077)
                     /gene="cysC"
                     /locus_tag="PLE93_00335"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF002059.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNH85521.1"
                     /translation="MSDSKGFTLWFTGLSGAGKTTISEIVARELTDRGSKLEILDGDI
                     VRENLSKGLGFSKEDRDINIRRIAFVADLLSRNGVPVITAAISPYRALRDEARELMGD
                     RFIEVYIEASVEVCAERDVKGLYAKAFSGEIKEFTGVSDPYEAPENPEIALKTAEEEP
                     EESAARLLAYLEERQLIPAVVAS"
     gene            complement(1081..2148)
                     /gene="sat"
                     /locus_tag="PLE93_00340"
     CDS             complement(1081..2148)
                     /gene="sat"
                     /locus_tag="PLE93_00340"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HNH85522.1"
                     /translation="MTELALQLSERQTADFEMIGNGGFAPLTGFQGSADWQRVCDEMR
                     TADGDYWPIPITLATDLECEVGDVVSLTSDEGKALGRITIEEIFERDVEHEAKSVYQT
                     NDQEHPGVAAIREEGARCLAGPIEVDALPDHEEAFQRRYLTPAESKQAFADRGWKRVV
                     AFQTRNPIHRAHEYLTKAALEVSDGLFIHPLIGKTKAGDIPADVRMKCYELLMEDYYP
                     NDRVILGVNPSKMHYAGPREAVLHAIVRRNYGCTHFIVGRDHAGVGDYYGTYDAQKIF
                     EGIDIDELGIEPLMFEHTFWCNECEGLASNKTCPHDAEAHLFLSGTKVREMLGNGEMP
                     PPEFSRPEVAQILIDAYKEDN"
     gene            complement(2242..2997)
                     /locus_tag="PLE93_00345"
     CDS             complement(2242..2997)
                     /locus_tag="PLE93_00345"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNH85523.1"
                     /translation="MEPSSTLTPAEAEAARKAEEATRVLAARFDAEAEAARVEPLDAS
                     QLLEWAFEQFGQDMYIACSFQKTSSVVMHLATEINPEARFFYIDTDFLFPETYETRDR
                     LAEHFGVEFERWHNISPEEQAREFGDQLWKRDPDACCGIRKVDPMARALASGVDCWVA
                     GIRREDSATRAGSPKFMWDRKFNIWKLNPIADWSERDVWTYLHKHGIPYNPLHDQGYP
                     SIGCTHCTRPVLPGQSSRDGRWSGSGKTECGIN"
     gene            complement(3007..4905)
                     /locus_tag="PLE93_00350"
     CDS             complement(3007..4905)
                     /locus_tag="PLE93_00350"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNH85524.1"
                     /translation="MEPTSAPSKPKPADKPKPKPGDKGIEQEDGKLQEGKGRLGTLTN
                     PETYENIPGHIIPIIEEEFVDFEGEANEFLDGNTPEDQFIGFRLKQGVYGQRQPGVQM
                     VRVKLPFGGVTPEQMDAFADAVETYAPLNKGHITTRQNIQIHHIPLNQMVHMLRDLAP
                     SGLSSREGCGNTVRNVTGDPWAGVAEDEIFDPTRYAGAYTRYFVRHPTTQLMPRKIKT
                     SFTGSDADRAMSGIHDIAFLSRIRDGVKGFEIRVGGGTSIMPRVAPTIYDFVEAENGD
                     YLKHAEAILRVYDRQAWLRPNRARARIKVLVDKVGIEEVRRMVDEELKGDWVNERDFT
                     IDHLVFDVDEEASLTEPLPPSGSPNGDRAEFDRYLEANVQPQRQEGFHTVEVKVVRGD
                     LTPEQFRGLGQIMRDYTGGFARMTVQQNIVLRWVREESLYDVWKRLAELGLDGVGPRR
                     ITDVVSCPGTDSCKMGITSSMGLNQAIQERVESMDITDPLTRDIHIKMSGCPNGCSQH
                     HIANIGFYGASLKVGDKTIPAYVPHLAGKYTDGDTIYGTRLKSRLPARRVPDAVERWI
                     RLYETDREEGEIFNAYVERVGTARMEEEVKDLTMPVDFSLDTMQMFIDWQRSDPYKVE
                     RGEGECAV"
     gene            complement(5169..5573)
                     /locus_tag="PLE93_00355"
     CDS             complement(5169..5573)
                     /locus_tag="PLE93_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH85525.1"
                     /translation="MSDEEATEVQPYERSSAEVAELIAAGARLIDVRTAHEHEAARLA
                     GSERIGLEELLDRRDEIEQDGTVIFYCRVGNRSGMAAQAFSEAGFDVVNLTGGIVQWI
                     EDGQPIEPADGYVAEPGEAAAILEARARAAGN"
     gene            complement(5708..5917)
                     /locus_tag="PLE93_00360"
     CDS             complement(5708..5917)
                     /locus_tag="PLE93_00360"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HNH85526.1"
                     /translation="MAVVKIIELVGSSRVSTDDAAQQALKQAQDSLRNIRAVDIVSTG
                     IRGENLDEYRAHVRVAFLIEGKDVT"
     gene            complement(5996..6199)
                     /locus_tag="PLE93_00365"
     CDS             complement(5996..6199)
                     /locus_tag="PLE93_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85527.1"
                     /translation="MSEITISQSTTPQEAAAIAAAIQRFSQDTAIAPATEPAGMNPWL
                     KAALEDGVSAKEAFGPGQPFGTL"
     gene            6284..>7386
                     /locus_tag="PLE93_00370"
     CDS             6284..>7386
                     /locus_tag="PLE93_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85528.1"
                     /translation="MKTARAARILFGVALLASAALLLHYRLDIGFVADDWAFLRDRSL
                     GGLDSLLIPENENIVILQAAVYRIFYILFGIQSSASLQIASLVFFLLSVVAFYAWIRE
                     RVGEWAAAIASIPILFLGTAYQDLLWAFQMGYTVSILAGLTALIALERNARFSQPVAL
                     VAILVSITTSSLGLPFLAGAAVILVSRDGWKGLFNYVLLAPLLVFLVWWAGWGRTADS
                     HLSLTNLLHAPAFVAESVKSVVMGLTGLHRTDSVAGDVLAWVVSGALFAAVSFSLIRR
                     KRLPLPLLVALAVGLAFWVLTAANQAPGMFTREADQSRYQYPGAVFLLMILAGAFDGY
                     RPGRNWLIGLASLAAVSVACSLGTLSEGRKILI"
BASE COUNT         1140 a         2285 c         2506 g         1455 t
ORIGIN      
        1 cctggatccc gccgcggtaa taggcgagag attcgggctc gaccccggcg tccagcagcc
       61 ttcccaccgc tctgggggtt gccgcacact gcggcccgtt gcagaagagc accgtcacgc
      121 ttgcgggatc gaacacctcc aggtggtcgg tgatcgtctc ccagggcaga accagcgagc
      181 cggggatcgt cccgctcgcc gatgcgtatt caggcagccg ggtgtcgatc agacgcccgc
      241 ccgcctcgat gtgcgcgatg acttccagct caccccaggt gggaatcccg gggtggagcc
      301 gctgcggctg gatcgtgccc caccaggggt cgatcgtgat ctgtccgtcg gcatcgacca
      361 gcgggacccg gctgggctcg tgcccggccg gcgcctcggc gcgttcgatg gtaaggaaaa
      421 cggacatggc caaagtatat cactacacaa ctaaacagtt gtttatagag ggaagaaggg
      481 gcgggctcga aagcccgccc cggcgaagta cgttttcgcg gttgcctacg aggcgacgac
      541 cgccgggatg agctggcgct cttccaggta ggcgagcagc ctggcagccg attcctccgg
      601 ctcctcttcc gcggtcttga gggcgatctc cggattctcc ggtgcctcgt acgggtcgga
      661 gaccccggtg aattccttga tctcgccgga gaaggccttg gcgtagagac ccttcacgtc
      721 gcgttcggcg cagacttcaa ccgacgcctc gatgtagacc tcgatgaagc ggtcacccat
      781 cagctctcga gcctcgtcgc ggagtgcccg gtagggcgag atcgcggcag tgatcaccgg
      841 gacgccgttg cgggaaagca ggtcggcgac gaaggcgatc cggcggatgt tgatgtcacg
      901 atcctccttc gagaagccga ggcccttcga gaggttttcg cgaacgatgt caccatcgag
      961 aatctcgagc ttggagccgc ggtcggtcag ctccctggcg acgatctcgg aaatggtggt
     1021 cttgccggca ccggagaggc cggtgaacca gagggtgaat cccttgctgt ctgacattgg
     1081 ttagttgtcc tccttgtaag cgtcaatcaa gatctgggcg acctcggggc gcgagaactc
     1141 gggcggcggc atttcgccgt tgccaagcat ctcgcggacc ttggtgccgg aaaggaaaag
     1201 gtgcgcttcg gcgtcgtgag ggcaggtctt gtttgaagcc aggccctcgc attcgttgca
     1261 ccagaaggtg tgctcgaaca tcagcggctc gatcccgagc tcgtcgatgt cgattccttc
     1321 gaagatcttc tgggcgtcat aggtgccgta gtaatcaccg acgccggcgt ggtcgcggcc
     1381 gacgatgaag tgggtgcagc cgtagttgcg acgcacgatc gcgtggagca cggcctcacg
     1441 ggggccggcg taatgcatct tcgaggggtt gaccccgagg atcacccggt cgttcgggta
     1501 gtagtcctcc atcagcagtt cgtagcactt catccgcacg tcagccggga tgtcgccggc
     1561 cttggtcttg ccgatcagcg ggtgaatgaa gagaccgtcg gaaacctcga gcgccgcctt
     1621 ggtcaggtac tcgtgggcgc ggtgaatcgg gttgcgggtc tggaaagcca cgacccgctt
     1681 ccagccgcgg tcggcgaagg cctgcttcga ctcggccggg gtcaggtagc ggcgctggaa
     1741 ggcttcctcg tgatcgggca gggcgtcgac ctcgatcggg ccggcgaggc aacgggcgcc
     1801 ctcctcgcgg atcgcagcga ccccggggtg ctcctggtcg ttggtctggt agaccgactt
     1861 cgcttcatgc tcgacgtcgc gctcgaagat ctcctcgatc gtgatccggc cgagcgcctt
     1921 gccttcgtcc gaggtcaggc tgaccacgtc gccaacctca cattcgaggt cggtggccag
     1981 ggtgatcggg atcggccagt agtcaccgtc ggcggtccgc atttcgtcgc agacgcgctg
     2041 ccagtcggcc gagccctgga atccggtaag gggtgcgaaa cccccgttcc cgatcatttc
     2101 gaagtctgcg gtttgacgct cggacagctg gagggcaagt tcagtcatgg tttggttcat
     2161 aaggaggagg gaatgttgac aggaacggtg aagaatcgga cagcctagcc gttatgggcg
     2221 gcaaatgccg tccgacagcg gctagttgat gccgcactcc gtcttgcccg agccggacca
     2281 gcgcccgtca cgggaagact ggccgggcaa aacgggacgg gtgcagtggg tgcagccgat
     2341 cgagggataa ccctgatcgt gcagggggtt gtaggggatt ccgtgcttgt gaaggtaggt
     2401 ccagacgtcg cgttcggacc agtcggcgat cgggttcagc ttccagatgt tgaacttcct
     2461 gtcccacatg aacttcggcg agccggcccg cgttgccgaa tcctcgcgcc ggatcccggc
     2521 gacccagcaa tcgactccgg atgcgagcgc ccgggccatc ggatcaacct tgcggatgcc
     2581 gcagcaggcg tccgggtcgc gcttccagag ctggtcaccg aactcgcggg cctgctcctc
     2641 ggggctgatg ttgtgccagc gctcgaactc gactccgaaa tgctcggcca gccggtctcg
     2701 ggtctcgtag gtctcgggga agaggaagtc cgtgtcgatg tagaagaagc gggcttcggg
     2761 gttgatctcg gtggcgaggt gcatcaccac cgagctggtc ttctggaagg agcaggcgat
     2821 gtacatgtcc tgcccgaact gctcgaaggc ccactccagc agctggctcg catccagcgg
     2881 ctcgacccgg gcggcctccg cctccgcgtc aaagcgggcg gccaggacgc gggtggcctc
     2941 ctcggccttg cgcgccgctt cggcttccgc cggagtgagt gtgctggacg gctccatcaa
     3001 ggtttcctag acggcgcact cgccttcgcc gcgctcgacc ttgtacgggt cggagcgctg
     3061 ccagtcgatg aacatctgca tggtgtcgag gctgaagtcg accggcatgg tcaggtcctt
     3121 cacttcttct tccatgcggg cggtgccgac ccgctcgacg taggcgttga agatctcgcc
     3181 ttcctcgcga tcggtctcgt agaggcgaat ccaccgctca accgcatccg gaacccggcg
     3241 ggcgggcagg cgggacttga ggcgggtgcc gtagatcgtg tccccgtcgg tgtacttgcc
     3301 ggcgaggtgc ggcacgtagg ccgggatggt cttgtcgccg accttcagcg acgcgccgta
     3361 gaagccgatg ttggcgatgt gatgctggct gcatccgttc gggcagccgc tcatcttgat
     3421 gtggatgtcg cgggtcagag ggtcggtgat gtccatcgac tcgacccgtt cctggatcgc
     3481 ctggttgagg cccatcgagg aggtgatgcc catcttgcag gagtcggtgc cggggcagga
     3541 gactacgtcg gtgatgcggc gcgggccgac gccatcgagc ccgagttcgg cgagccgctt
     3601 ccagacgtcg tagagggatt cctcgcgaac ccagcgcagg acgatgttct gctggacggt
     3661 catccgggcg aagccgccgg tgtagtcccg catgatctgg ccaaggccgc ggaactgctc
     3721 cggggtgagg tcgccacgga ccaccttgac ctcgaccgtg tggaagccct cctggcgctg
     3781 cggctggacg ttcgcttcga ggtagcggtc gaattcggcg cggtcgccgt tcggcgagcc
     3841 gctcgggggc aggggctcgg tcagcgaggc ttcctcgtcg acgtcgaaga ccaggtggtc
     3901 gatcgtgaaa tcacgctcgt tcacccagtc gcccttcagt tcctcgtcga ccatgcggcg
     3961 gacctcttcg atcccgacct tgtcgaccag gaccttgatc cgggcgcggg cccggttcgg
     4021 acgcagccag gcctggcggt cgtagacccg caggatcgct tcggcgtgct tgagataatc
     4081 gccgttttcg gcctcgacga agtcgtagat cgtgggggcg acgcggggca tgatcgaggt
     4141 gccgccgccg acccggatct cgaagccctt gacgccgtcc ctgatccggc tgaggaaggc
     4201 gatgtcatgg atgccgctca tcgcccggtc ggcgtcggaa ccggtgaagg aagtcttgat
     4261 cttccgcggc atcagctggg tcgtggggtg ccggacgaag tagcgggtgt aggcgcctgc
     4321 gtacctggtc gggtcgaaga tctcgtcttc ggcgacgccg gcccaggggt cgccggtcac
     4381 gttgcggacc gtgttgccgc agccctcgcg ggacgagagg ccgctggggg cgaggtcgcg
     4441 cagcatgtgg accatctggt tcagcgggat gtggtggatc tggatgttct gccgggtcgt
     4501 gatgtgaccc ttgttgagcg gcgcgtaagt ctcgaccgcg tcggcgaagg catccatctg
     4561 ctccggggtg acgccgccga agggcagctt cacgcgaacc atctgcacgc cgggctggcg
     4621 ctggccgtaa acgccctgct tgaggcggaa cccgatgaac tggtcctcgg gggtgttgcc
     4681 gtccaggaac tcgttcgcct caccctcgaa atcgacgaac tcctcttcga tgatcgggat
     4741 gatgtgaccg gggatgttct cgtaggtctc cgggttggtc agcgttccca gccggccctt
     4801 gccttcctgg agcttgccgt cctcctgctc gatccccttg tcaccgggct tcggcttggg
     4861 cttgtcggcg ggcttcggtt tggacggggc cgaagtgggc tccaattggg tttcctcctg
     4921 ctgcgaaaaa ggcttggttg ctgacgtggg ctgacaacgg tagcaaaaag accaaaacct
     4981 gaccggattt catgtaatta gaagtcgggc cggtccgacc gaccctcgag tggctacccg
     5041 tacctgtcta tggaccggtt ctgcagggtt cgggtcggcg gtgcgtcccg aaaacctctg
     5101 gaatgaggag cgcagcgacg gttttcggga agtactgccg ttccgggccc cgtcgtcaag
     5161 ccgctcggct agttgcctgc tgctctggcg cgggcttcga ggatggcggc ggcttcgccc
     5221 ggttcggcca cgtagccgtc ggccggctcg atcggctggc cgtcctcgat ccactggacg
     5281 atgccgccgg tcaggttcac cacgtcaaag ccggcttcgg agaatgcctg ggccgccatg
     5341 ccggaacggt taccgacccg gcagtagaag atgaccgttc catcctgctc gatctcatcg
     5401 cggcggtcga gcagctcctc gagcccgatc cgctcggaac cggccaggcg cgcagcctcg
     5461 tgctcatgcg cggtccgcac atcgatcaaa cgcgcaccag ccgcaatcag ttccgccacc
     5521 tcggcagaac tccgctcgta aggctggact tcggttgctt cttcgtcact catgggtccg
     5581 gaaaggtagc agggaggaca ggcctggccc acccctggca gagcccctcc caaccatcag
     5641 ttacttcaat tgggaggggc gggtgtcaac tggagggcgg gtcggacccc gcccgactcc
     5701 tgttctccta agttacgtcc ttgccttcga tcaggaacgc gacgcggacg tgggcgcggt
     5761 actcgtcaag gttttccccg cggatgccgg tcgagacgat gtccacggcg cggatgttgc
     5821 gaagcgaatc ctgggcctgc ttgagcgcct gctgggctgc gtcatcggtt gaaacccgtg
     5881 agctgccaac cagctcgatg atcttcacta ccgccatgaa ctcctcctcg gtcgatttga
     5941 ctcgatgcta gttgctcgcg cgaagaagtg cgagggggcg gagcagcggg actcgtcaga
     6001 gcgtgccgaa gggctggccg gggccgaaag cttccttggc cgagaccccg tcttcgaggg
     6061 cggccttcag ccagggattc atcccggccg gttcggtcgc gggggcgatc gcggtgtcct
     6121 gcgaaaagcg ctgaatcgcc gcggcgatcg cggcggcttc ctgcggggtc gtggactggc
     6181 tgatcgtgat ttcgctcatg cggcgtcaga ttctatgctc gcccgctccc gcgacggccg
     6241 ccgataggct gcgcccatcg tgggagccgg tttcagtgag ggcatgaaga ctgcgcgagc
     6301 cgcccggatt ctcttcgggg tggcgcttct ggcatcggcc gccttgctgc ttcactaccg
     6361 gctcgacatc ggtttcgtgg ccgacgactg ggcctttctg agggatcgca gcctgggcgg
     6421 gctcgattcg ctgctgatcc cggagaacga gaacatcgtc attctgcagg cggcggtcta
     6481 ccggatcttc tacatcctgt tcggaatcca gtcctcggcg tcgcttcaga tcgccagtct
     6541 cgtcttcttc ctgctgagcg tggttgcctt ctacgcgtgg attcgcgagc gggtgggcga
     6601 gtgggcggcg gcaatcgcat ctatcccgat cctcttcctc ggcaccgcct accaggacct
     6661 gctctgggcc ttccagatgg ggtacacggt ttcgatcctg gccgggctga cggccctgat
     6721 cgccctggag cgaaacgcaa ggttcagcca gccggtggct ctcgtcgcca ttctggtgtc
     6781 gatcacgacc tcgtcgctgg ggctcccctt cctcgccggt gccgccgtca tcctcgtctc
     6841 gagggacggc tggaagggcc tcttcaacta cgtgcttctg gcgccccttc tggtgtttct
     6901 cgtttggtgg gcgggctggg gaaggacggc tgacagtcac ctgagcctga ccaatctcct
     6961 ccacgccccg gccttcgtgg cggagtcggt caagtcggtg gtgatgggat tgaccggact
     7021 ccaccgcacc gattcagtgg ccggggacgt gctggcctgg gtcgtttcgg gggcactctt
     7081 cgccgcggtc tcgttcagcc tgattcgccg aaaacggctg ccgctgccgt tgctcgtcgc
     7141 gctggcggtc gggctcgcct tctgggtcct gaccgctgcc aatcaggcgc cggggatgtt
     7201 cacgcgggaa gccgaccaga gccggtacca gtacccgggg gcggtctttt tgctgatgat
     7261 cctggccgga gccttcgacg ggtaccggcc ggggcggaac tggctgatcg gcctggcttc
     7321 gctggccgcg gtctcggtcg catgcagcct cggcaccctt tccgaaggac ggaagatcct
     7381 gattga
//
LOCUS       DAOQFI010000009         7103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_3816_length_7103_cov_3.778802, whole genome shotgun sequence.
ACCESSION   DAOQFI010000009 DAOQFI010000000
VERSION     DAOQFI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3816_length_7103_cov_3.778802"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..337)
                     /locus_tag="PLE93_00375"
     CDS             complement(2..337)
                     /locus_tag="PLE93_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85529.1"
                     /translation="MADHGLSNHHSSTLAEIFDHQKHSIEWRKVESLLNAVGTVTRKH
                     NGHLKVEVGPETETFEVPHGKEINRQQLVDIRRMLSEAGYGPDGKAVEDQRDRDYRDN
                     RWGDPVDEA"
     gene            complement(419..>1681)
                     /locus_tag="PLE93_00380"
     CDS             complement(419..>1681)
                     /locus_tag="PLE93_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH85530.1"
                     /translation="GCPISMTFSVIPSLRNQPEVAEEWEPRFTSLEYDGKLKPADQKP
                     GCLAGMAMTEKQGGSDVRANTTRAVPLNGGGPGGEYEITGHKWFCSAPMCDAFLVLAQ
                     TDAGVSCFLLPRFTPNGERNRFHIQRLKEKLGNKSNASSEIEFRGAWARMIGEDGRGI
                     RTIIEMVSHTRLDCAIGSSAGMRTGVAQATHHAMNRSAFGKKLIDQPLMENVLADLCV
                     ESEAATATVMRLARAYDEAAAGDEGAALLQRLATPVLKYWICKRAPWHAVESLECFGG
                     NGYAEESGMPRLFRESPLMSIWEGSGNVQCLDALRAMVKSPASYEAFFSEVGEAAGAD
                     SRLDSFVEKLQQEITGEPETLEIRARRVVEGMGLAFQGSMLVRHGDPAVADAFCASRL
                     AGDWGEAFGTLPAGTDFKTIIQRSAPPV"
     assembly_gap    1682..1781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2010..3224
                     /locus_tag="PLE93_00385"
     CDS             2010..3224
                     /locus_tag="PLE93_00385"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNH85531.1"
                     /translation="MSKIKVTNPIVELDGDEMTRIIWSFIKEQLILPYLDVDLKYYDL
                     GIESRDDTDDQITVDAANAIKEHGVGVKCATITPDEARVEEFDLKEMYRSPNGTIRNI
                     LGGVIFREPIVISNVPRLVPGWTKPIIIGRHAFGDQYRATDMVVEGEGTLTMTFTPKD
                     EGEPIEMHVHDFPGGGVAMAMYNHDDSIRDFARASFRYGLERGYPVYMSTKNTIMKRY
                     DGRFMILFQEVFDDEFKDEFDQAGLTYEHRLIDDMVASALKWEGGYVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREHQKGNATSTNPIASIFA
                     WTRGIAARGRMDETPEVSQFGETLEKVCIETVESGKMTKDLALLVGPDQEWQTTQEFL
                     ASIDENLQKAMA"
     gene            3255..4241
                     /locus_tag="PLE93_00390"
     CDS             3255..4241
                     /locus_tag="PLE93_00390"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNH85532.1"
                     /translation="MSSTAKVTVTGAAGQIGYALLFRIASGQMLGPDTKVHLSLLEIP
                     AAVKAAEGTAMELDDCAFPLLEKISISDDPKEAFDGVNVGLLVGARPRGPGMERADLL
                     EANGGIFKPQGEAINAGAADDVKILVVGNPANTNALIAASAAPDVPKERFTAMMRLDH
                     NRAIAQLANKLGRSVSDVKRMTVWGNHSATQYPDIGNALVDGAPAPAQVDDEVWVREE
                     FIPRVAKRGAEVIDARGASSAASAANAAIDHVRDWVLGTPSEDWVSMGIPSDGSYGVP
                     EGIIAGFPVTCSGGEYSIVEGLEIDEFSREKIDANAAELMEERNAVADLGLL"
     gene            4310..5083
                     /locus_tag="PLE93_00395"
     CDS             4310..5083
                     /locus_tag="PLE93_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85533.1"
                     /translation="MRGKFRKPTYAEIVATLALFIALGGISYAAIKIPNNSVGTKQLK
                     KSAVNSAKVRDRSLLAKDFRKGQIPRGATGAQGAPGMIGPTGAAGATGASGIPGAVGA
                     TGPTGAAGVTGENGDPGQPGGAGPTGPTGVTGPSTTAVSSGVVVNLGLGPDYFPISGA
                     SSPQGAPDTVASLSPGVPVTASNLDVKLTAAPFAGFPRIVSLLDDGIAVLSCVIASGT
                     TCHADTSALIDPGSELVMVSSVLAFPSITTARFGFTLGP"
     gene            5134..5934
                     /locus_tag="PLE93_00400"
     CDS             5134..5934
                     /locus_tag="PLE93_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HNH85534.1"
                     /translation="MGNRGKGLKGFKSKLRRPNFAETIAVIALFVALGGASYAAIKIP
                     KNSVGTKQLKKNAVTSAKVKNRSLLAADFKAGQLPAGATGATGLVGPRGATGEQGIPG
                     FPGTPGTPGINGIDGLPGSPGPAGATGATGATGSTGATGTGGFSAMTGRTSLGSSTQF
                     FAVSGSSVAGATATGLGMITPAVPVTLKNLMVVTDTAPGTGITRTFVIQEDGINVISC
                     TLGGIATACFNTSGGGVINAGSTLTMFDTVTGGSAAPSEARWGFTIGS"
     gene            5961..6926
                     /locus_tag="PLE93_00405"
     CDS             5961..6926
                     /locus_tag="PLE93_00405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNH85535.1"
                     /translation="MSERGKVVILRELLPAGRQLLEEKFQVTSLGLDADRSQMKAAAA
                     GADGLVTDLTFAVDDELLGAAGPQLKVVANFGVGYDNVDLAACAARGVTVTNTPGALT
                     NATAELALALAMAAARDLPAAEKDLREGAWQGWDPRRWRGFELSGASIGVVGAGRIGG
                     RFMELVAGFGGERLYTSRQPKPDLENRLGAERVELDELLARSDLISLHLPGSRESRHL
                     IDERALRLMKRTAVLVNTGRGTLVDSAALIRALKEDRIGAAGLDVYEGEPDFDPGFLT
                     APRVALAPHIGSATYRSRDEMSRLVAANVIAVLEGREPPNPVPGV"
BASE COUNT         1257 a         2266 c         2300 g         1180 t
ORIGIN      
        1 gtcaggcctc gtctacgggg tcgccccagc ggttgtcccg gtaatcgcgg tcgcgctggt
       61 cctcgacggc cttgccgtcc ggcccgtatc ccgcttcgct cagcatccgc cggatgtcga
      121 ccagctgctg gcggttgatc tccttgccgt ggggaacttc gaaggtctcg gtttccggac
      181 ccacctccac cttcaggtgg ccgttgtgct tcctggtcac cgtgccgacc gcgttcagca
      241 gcgattcgac cttgcgccac tcgatcgagt gcttctggtg atcaaagatc tcggcgaggg
      301 tgctgctgtg gtggttgctc agaccgtggt cggccatgga gttcctttcg aagttgacgg
      361 aaggatgaat atcacctgcc cacctgcgaa caggtggcca tggcccgtcg gttatcgatt
      421 agaccggggg agcggagcgc tggatgatcg tcttgaaatc ggttccggcg ggaagggtgc
      481 cgaaggcctc gccccagtcg ccggccagac gggaggcgca gaaggcgtcg gccacagcag
      541 ggtcaccgtg gcggacgagc atcgacccct ggaaggcgag tcccatgcct tcgactacgc
      601 ggcgggccct gatctcgagc gtctcgggtt caccggtgat ctcctgctgg agcttctcga
      661 caaacgaatc gagacgtgag tcagcgccgg cggcctcgcc gacctcgctg aagaacgcct
      721 cgtaggaagc ggggctcttg accatcgctc gcagtgcgtc gaggcactgg acgttgccgg
      781 agccttccca gatcgacatc agcgggctct ccctgaacag gcggggcatt cccgactctt
      841 ccgcgtaacc gttgccaccg aagcattcga gcgattcgac ggcatgccac ggggcgcgct
      901 tgcagatcca gtacttgagg accggagtgg ccaggcgctg aaggagagcg gcaccttcgt
      961 caccggccgc ggcctcgtcg tatgcccggg ccagacgcat gaccgttgcg gttgcggcct
     1021 cggactcaac acagaggtcg gccagcacgt tctccatcag cggctggtcg atcagcttct
     1081 tgccgaaggc ggaacggttc atcgcgtggt gggttgcctg cgccacgccg gttcgcattc
     1141 ctgcgctcga gccgatcgcg cagtcaaggc gggtgtggct gaccatttcg atgatggtcc
     1201 ggatgccccg gccgtcctcg ccgatcatgc gtgcccacgc tccccggaac tcgatttcag
     1261 atgaggcatt cgacttgttg cccagcttct ccttcagacg ctggatgtgg aaccggttgc
     1321 gctcgccgtt cggggtgaaa cgcggcagaa gaaagcagga aacgccggca tcggtctggg
     1381 cgagaacgag gaaggcgtcg cacatcgggg cggagcagaa ccacttgtga ccggtgatct
     1441 cgtattcgcc gccggggccg ccaccgttca gcggaaccgc gcgagtggtg tttgcccgga
     1501 cgtcggagcc accctgcttc tcggtcatcg ccatcccggc caggcagcca ggcttctggt
     1561 ctgccggctt cagcttgccg tcgtactcga gggaggtgaa gcggggctcc cattcctcgg
     1621 cgacttcagg ctgatttcga agtgagggga tcaccgagaa ggtcatcgag atcgggcagc
     1681 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggaaggtcg atctcgtaga
     1801 ggttgacgtt ctccagcgga gtcgactgat tgaagaccgt atgggtctcg tgctgcggag
     1861 cttcggcggt ttccagcatg gagtaatggt ctcacagccc cggcgcattg aggcacggat
     1921 aactacccat tgacgactgc aaggcgtttc gctctatagc atcgctgcct gcaagagcct
     1981 tacgccaaaa tcgaagggaa gtaccagtcg tgtcgaagat caaagtgacc aacccgatcg
     2041 tcgagctgga tggcgacgag atgacccgca tcatctggtc gttcatcaag gagcagctga
     2101 tccttcccta tctcgacgtc gatctgaagt actacgacct cgggatcgaa agccgcgacg
     2161 acaccgatga ccagatcacg gtcgacgccg ccaacgcgat caaggagcac ggggtcgggg
     2221 tcaagtgcgc gaccatcact ccggacgagg cccgggtcga ggagttcgac ctgaaagaga
     2281 tgtaccgctc gccgaacggc acgatcagga acatcctcgg cggcgtgatc ttccgcgagc
     2341 cgatcgtgat ctcgaacgtg ccgcgcctcg tgcccggctg gaccaagccg atcatcatcg
     2401 ggcgtcacgc cttcggcgac cagtaccgcg ccaccgacat ggtggtcgag ggcgaaggca
     2461 ccctgaccat gaccttcacc ccgaaggacg agggcgagcc gatcgagatg catgtccatg
     2521 acttccccgg tggtggcgtc gcgatggcga tgtacaacca cgacgattcg atccgtgact
     2581 ttgcccgggc ttccttccgt tacggcctcg agcgcggcta cccggtctac atgtcgacca
     2641 agaacacgat catgaagcgc tacgacggcc ggttcatgat cctcttccag gaagtcttcg
     2701 atgacgagtt caaggacgag ttcgaccagg ccgggctcac ctacgagcac cgcctgatcg
     2761 acgacatggt cgcctcggcc ctgaaatggg agggcggcta cgtatgggcc tgcaagaact
     2821 acgacggcga cgtccagtcc gacaccgtgg cccagggctt cggctcactc ggcctgatga
     2881 cctcggtcct gatgaccccg gacggaaaga cggtcgaggc ggaagccgcc cacggcaccg
     2941 tgacccgcca ttaccgcgag caccagaagg gcaacgcgac ctcgaccaac ccgatcgcct
     3001 cgatcttcgc ctggacccgg gggatcgccg cccggggccg catggatgag acccccgagg
     3061 tcagccagtt cggcgagacc ctcgagaagg tctgcatcga gacggtcgag tccggcaaga
     3121 tgacaaagga cctcgccctg ctggtcggtc ccgaccagga gtggcagacc acccaggaat
     3181 tcctggcctc aatcgacgag aacctccaga aggcgatggc ctagggcccg ccaccagaaa
     3241 ccaaggagaa gaacgtgagt tcaaccgcca aggtaaccgt gaccggcgct gccggccaga
     3301 tcggttacgc cctgcttttc cggatcgcca gtggacagat gctcggtccc gacaccaagg
     3361 tccacctgag cctgctcgag atcccggccg ccgtgaaggc cgccgaaggc accgccatgg
     3421 agctcgacga ctgcgccttc ccgctgctcg agaagatcag catctcggac gacccgaagg
     3481 aagcgttcga cggcgtcaac gtcggactgc tggtcggcgc ccgtccgcgt ggaccgggaa
     3541 tggaacgggc cgatctgctc gaggcaaacg gtggcatctt caagccgcag ggtgaggcga
     3601 tcaacgccgg cgccgccgat gacgtgaaga tcctcgtggt cggcaacccg gccaacacca
     3661 acgccctgat cgctgcctcg gccgctccgg acgtgccgaa ggagcgcttc accgcgatga
     3721 tgcgcctcga ccacaaccgg gcgatcgccc agctcgccaa caagctcggc cgctccgttt
     3781 ctgacgtcaa gcggatgacc gtgtggggca accattcggc cacccagtac ccggacatcg
     3841 gcaacgccct ggttgatggc gctccggccc ccgcccaggt cgatgatgaa gtctgggtcc
     3901 gcgaggagtt cattccgcgg gtagccaagc gtggcgccga agtaatcgac gcccgcggag
     3961 cctcaagtgc cgcgtccgcg gccaacgcgg cgatcgacca cgtccgcgac tgggtgctcg
     4021 gcaccccgag cgaggactgg gtctcgatgg gcatcccttc ggacggctcc tacggcgtgc
     4081 cggagggaat catcgccggc ttcccggtca cctgctccgg cggcgagtac tcgatcgtcg
     4141 aaggcctcga gatcgatgaa ttctcacgcg agaagatcga cgccaacgcc gccgagctga
     4201 tggaagagcg gaacgcggta gccgacctcg gcctgctcta ggcccaagca gttcgccgtt
     4261 tcatgaaagc ccgggccacg acccgggctt tcgtggttat ggtggaaaca tgcgggggaa
     4321 gttccggaaa ccgacctatg cagaaattgt cgcgacgctg gcgctcttca tcgccctcgg
     4381 cggcatttcg tacgcggcga tcaaaatccc gaacaacagc gtcggcacca aacaactgaa
     4441 gaagagcgcg gtcaactcgg ccaaggtccg ggaccgctcg ctgctcgcca aggatttcag
     4501 gaagggccag attccccgcg gggccaccgg cgcccagggt gctccgggca tgatcggccc
     4561 gaccggcgcc gcaggggcca ccggcgccag cggaatcccc ggtgcggtcg gtgctaccgg
     4621 cccgaccggt gcggccgggg tcactggcga gaacggcgat ccggggcaac ccgggggtgc
     4681 cggaccaacc ggtccgaccg gggtgaccgg gcccagcacg accgcggtca gttccggggt
     4741 cgtggtcaat ctcgggctcg gccccgacta cttcccgatc agcggggctt cctcgcctca
     4801 gggagcgccc gacaccgtgg cctcactctc gcccggggtt cccgtcaccg cttccaacct
     4861 cgacgtgaaa ctgaccgccg cccccttcgc gggatttcct cgcatcgtca gcctcctgga
     4921 tgatggtatc gcggtgctca gttgcgtcat agcctcgggt accacttgcc acgcggatac
     4981 ttcagcgctg atcgatcccg gaagcgagct ggtgatggtc agctcggtcc tggcgttccc
     5041 gagcatcacc accgcgcgct tcggcttcac gctcgggcca tgatccgatc gaaggctctg
     5101 gcaatttccg ggctctgcgg gtagggtcgg cacatgggga accgggggaa gggcctgaag
     5161 ggattcaagt cgaagcttcg tcggccgaac ttcgccgaaa cgatcgcggt cattgcgttg
     5221 ttcgttgcgc tgggcggcgc ctcgtatgcg gcgatcaaga tcccgaagaa cagcgtcggc
     5281 accaaacagc tgaagaagaa cgcggtgacc agcgccaagg tcaagaaccg ttcgctgctc
     5341 gcggccgatt tcaaagccgg ccagctgccg gccggagcga ccggtgcgac cggactggtc
     5401 ggccccaggg gggcgaccgg agaacaggga attccgggat tccccgggac gccggggacg
     5461 cccgggataa acggcatcga cggtttgccc ggttcgccag gccccgcggg agcgacggga
     5521 gcgaccggag ccaccggttc caccggtgcg accggtactg gcggattctc ggcgatgacc
     5581 ggacgtacct cgcttggcag ctcgactcag ttcttcgcgg tttcggggtc cagtgtcgcc
     5641 ggcgccaccg caaccggcct ggggatgatt acgccggcag ttccggtcac gctcaagaac
     5701 ctgatggttg tcaccgacac cgcgcccggc accggaatca cccggacttt cgtgattcag
     5761 gaagacggaa tcaacgtgat cagttgcacc ctgggcggca ttgccactgc ctgcttcaac
     5821 acttccggtg gcggcgtcat caacgcggga agcaccctga cgatgtttga cacggtgacc
     5881 ggcggctcgg cggctccttc cgaagcccgc tggggattca cgatcggttc ctgaggcgcc
     5941 ggccgtgtag cgtctggcct gtgagcgaac gggggaaagt cgtgattctg cgcgagctgc
     6001 tgccggccgg gcgccagttg ctcgaggaga aatttcaggt gacttcgctg ggtctcgacg
     6061 ccgaccggtc acagatgaaa gccgcggctg ccggagccga tggccttgtc accgacctga
     6121 ccttcgcggt cgatgacgag ctgctgggtg ccgccggccc gcaactcaaa gtggtggcga
     6181 atttcggggt gggctacgac aacgtcgacc ttgccgcctg tgccgcgcgc ggagtcacgg
     6241 tgaccaacac gcccggtgcc ctgaccaacg ccacggcgga gcttgcactc gcgctggcga
     6301 tggctgcggc ccgcgacctg ccggcagccg agaaggacct gcgggaaggg gcctggcagg
     6361 ggtgggaccc gaggcgatgg cgcggcttcg aactcagcgg ggcgagcatc ggagtggtcg
     6421 gtgccggccg gatcggtggc cgcttcatgg aactcgttgc cggtttcggc ggggagcggc
     6481 tctacacctc gcgccagccc aagcccgacc ttgaaaaccg gctgggagcc gaaagagttg
     6541 aactcgacga gctgctcgcc cgctcggacc tgatcagcct tcacctgccg ggcagccggg
     6601 aatcccgcca cctgatcgac gaacgcgcgc tgaggttgat gaaacggacc gcggtgctgg
     6661 tcaacaccgg tcgcggcacc ctggttgatt ccgcggccct gatcagggcg ctgaaggaag
     6721 accggatcgg cgcggcgggg ctcgatgtgt acgagggcga gcctgatttc gatcccgggt
     6781 tcctgaccgc gccccgggtt gcgctggctc cccacatcgg ctcggcaacc taccgctcgc
     6841 gggacgaaat gtcccgactg gttgccgcca acgtgatcgc cgtcctcgag ggccgggaac
     6901 cgccaaaccc ggtaccgggc gtttgatcat ttcggcctgc tgaagaccag cgaccgttcg
     6961 cccgggaagg ccggtcagga tccgcgatcg gccttgcgcg aacgcggaac ggtgcggaag
     7021 acgtggtccc agaacggggt cgagacgccg tacccgtagt gatggtcctg gaagtgatgg
     7081 cgcatgtgat gttcacgcac ctt
//
LOCUS       DAOQFI010000010         7047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_3867_length_7047_cov_3.606550, whole genome shotgun sequence.
ACCESSION   DAOQFI010000010 DAOQFI010000000
VERSION     DAOQFI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3867_length_7047_cov_3.606550"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PLE93_00410"
     CDS             complement(<1..330)
                     /locus_tag="PLE93_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85536.1"
                     /translation="MANGTPMKLAAPHGEADQNWLRARLEELERIDRPSASAGERAAA
                     EWLVSRFSELGAEARIETEKAHGTYWWPIGIGTALGAAGGIAATRGRRLAGALLGAAG
                     AAAIATDF"
     gene            392..1540
                     /locus_tag="PLE93_00415"
     CDS             392..1540
                     /locus_tag="PLE93_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNH85537.1"
                     /translation="MFGGANSITLFRVAGIRIAVDWSWFIFLFIVIVWLSGFYGDILD
                     EPGSTQSYGLALASAIGFFGSILLHELGHAFEAKRSGIGIRTIRLWLFGGVAEMDQES
                     ESPGTEFRVEIAGPVVTLLIAVILCVIGIQAAGFDNFRDALEMRITAETNGPITMVAW
                     LAAVNILVLGFNLLPAFPMDGGRIVRSIAWKITGKRSAATRFAAGLGRIFGFIFIGLG
                     LLWIFNGNLFSGLWLAMIGFLVNSSARAASRSTVGDKLEGITVSQVMDTEPVSIPAEI
                     DCARALEEYFLRFGWSWFPVVDRHGSFLGMAVRDRLEAVPEVERDATPVISLVEINPG
                     FSVRSDAGLDRLLGNPQLRRFGALMVTDDQGKLAGVVTVEQLGRALQA"
     gene            1594..2841
                     /locus_tag="PLE93_00420"
     CDS             1594..2841
                     /locus_tag="PLE93_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNH85538.1"
                     /translation="MAVATFLIVLALIASERVHRTKVALLGAAIVVLFVGQYNEELAI
                     ESVEFATLGLLAGMMILVYLTQQSGVYDYIAVKAGQLSRGRPFLLVVMLVTTVAVMSG
                     FLDNLTTILLIVPITFLLADTLDINPMPLILIEVMAANIGGAATLIGDPPNIMIGNSA
                     NLSFNDFLINNMPGVVVILATVTVGLYFAYRSQLQVAEENRKYVQELDAAASIRDHDE
                     LRRTLPVLIGTIILFFLHQYIHVEPATIALTGAAVGLMVTKTPIEEALEKIEWTTLFF
                     FIGLFVMVGALEVTGAIEKLAEGITSVTNGNRDAELVGIIWTSSLVGGIVDNIPLTAA
                     MIPVVEDIKAGSTDHAYWWALSLGACFGGNLTLISAACNVAASGMAEKAGRPIGFWTF
                     LKVGFPVTVASTVMATAYVLLRF"
     gene            2877..3353
                     /locus_tag="PLE93_00425"
     CDS             2877..3353
                     /locus_tag="PLE93_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNH85539.1"
                     /translation="MADHIVRSVIREVKPLKTSDPVGYAARLVIESGLPALPVVDEHG
                     KYAGLFGEREFMTAVFPGYVGTLMSARMIRRSLDETIERRIGSQEEPVRGYLTTDPVL
                     VEDDYADTQLAETFLHHRVMVVPIATEGRVHAVVPRSEFFQALYERIADIAEDFGD"
     gene            3394..4233
                     /locus_tag="PLE93_00430"
     CDS             3394..4233
                     /locus_tag="PLE93_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNH85540.1"
                     /translation="MPWNAEKPADQMDGNWLITEGDPESAVILHVPHASRAFPTRELN
                     RLVVDREGLDAELDAITDADTERLAAEAAGLAELRPWIFENRYSRLLVDPERFPDDRE
                     EMLEAGMGPVYLKTTTGAEFRRADEADDERLMSEYYRPYASAFERLVEQRLERLGRVL
                     LIDVHSFPRDPLPYELHSGEARPALCIGTDKFHTPQWLVDAISGLWPDTVELDQPFRG
                     CYVPLSFLESESRIAAVMLELRRDVVARWAGPDSPGRRGEPGLPGFVAAVADLFSRYD
                     PTG"
     gene            complement(4230..5258)
                     /locus_tag="PLE93_00435"
     CDS             complement(4230..5258)
                     /locus_tag="PLE93_00435"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNH85541.1"
                     /translation="MDESRYRVGVLGATGAVGSTILEILHERSFPASEVVAFASERSA
                     GKKVPFGEGELTCVVPDDESIQGIDILISSAGGSVSAEWAPKFSEAGAVVVDNTSYWR
                     MHEDVPLVVAEVNPEAARDHKGIIANPNCTTMVMMLVLAPIHREARLERLILSSYQAV
                     SGTGQKAIAELTEQAGQVLAGETVSYPAVYPHQIAFNVIPQVETFKDGDDYTTEERKV
                     MAETRKILGLGDEVKISATCARVPVFTGHSESLNIETREDLSPDRAREILGAAENVIV
                     VDNPGAGKYPTALDAAGRDETLVGRIRRDPGQEKTLNLWLAGDNLRKGAATNAVQVAE
                     LLVKNDWL"
     gene            complement(5261..6544)
                     /locus_tag="PLE93_00440"
     CDS             complement(5261..6544)
                     /locus_tag="PLE93_00440"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNH85542.1"
                     /translation="MPERDDIVVMKFGGTSVAGPEEIKRAARRMVAAREAGNSVVAVL
                     SARGKTTDELVASAYEISDRPDPREMDMLLSTGERISCALAAMAINDMGHKAVSLTGS
                     QAGIVTDSSHTKAKIIDVKADRIREGLGEDKIVLVAGFQGVSTDSRDVTTLGRGGSDT
                     TAVALAAALGASVCEIYTDVTGVFSADPRIVPDARKLPVVSFEEMLDMAASGAGVLQL
                     RSVEYARNYNIPIHCRSSFEDLPGTLVLPESETMERPIITAVTHSTDEARVAVIGVKD
                     EPGIAGRIFGALADANVNVDVIIQNEPVHEGALADLSFTVETGDLATATDAIASLGDI
                     SQEVRTDEQIGKVSIVGAGMRSHPGVAAKVFEVLGEKEINIEMISTSSIRISCVIQAD
                     QVPEAVRSLHAAFDLGEEQILAEAPTGEHRPVTPS"
     gene            6610..6966
                     /locus_tag="PLE93_00445"
     CDS             6610..6966
                     /locus_tag="PLE93_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNH85543.1"
                     /translation="MTDGSLRCPECGSEHTYELGGFSVCSLCAHEWDPAHDRTEIAGD
                     SAPEIRDSVGNVLTDGDTVTVIKNLKVKGASGPIKAGTKVKGIRLVNGVGDHDIDCRI
                     DGFGQMQLKSSVVKKA"
BASE COUNT         1214 a         2226 c         2288 g         1319 t
ORIGIN      
        1 gaagtcggtg gcgatcgccg cggcaccggc cgccccgagc agggcaccgg cgaggcgcct
       61 gccacgggtc gccgcaatgc cgcccgcggc tccgagcgcc gtcccgatac cgatcggcca
      121 ccagtaggtt ccgtgggcct tctcggtctc gatccgggct tccgctccga gttcagagaa
      181 ccggctgacc agccactcgg cggcagcgcg ctcccccgcc gaagccgaag gacgatcgat
      241 tcgctcgagc tcctcgagtc gggcacggag ccagttctgg tccgcttcgc cgtgcggcgc
      301 ggcaagtttc atcggtgtcc cgtttgccat ggaggagcaa gatagccgtt gcttggcccg
      361 ccccgttcac gcgggtccat acgatgtggg ggtgttcggt ggcgcaaact cgataactct
      421 cttccgggta gccggaatcc ggatcgcggt cgactggtcg tggttcatct tccttttcat
      481 cgtgatcgtc tggctctcgg gcttctacgg cgacatcctg gacgagcccg ggagcaccca
      541 gtcctacgga ctggccctgg ccagcgcgat cggtttcttc ggctcgatcc tgctccacga
      601 actcggtcac gcattcgagg cgaagcgcag cgggatcggc atccggacga tccgcctctg
      661 gctgttcggc ggggtcgcag aaatggacca ggagtccgag tcgccgggaa cggaattcag
      721 agtggagatc gccggaccgg tggtgacctt gctgatcgcc gtgatcctct gcgtgatcgg
      781 gattcaggcg gccgggttcg acaacttccg tgacgccctc gagatgcgga tcaccgcgga
      841 gaccaacggc ccgatcacca tggtcgcctg gctggcagcg gtcaacatcc tcgttctcgg
      901 tttcaacctg ctccccgcct tcccaatgga cggtgggcgg atcgtccgct cgatcgcctg
      961 gaagatcacc ggcaagcgca gcgccgcgac ccggttcgct gccggtcttg gtcgcatctt
     1021 cgggttcatc ttcatcgggc tgggcctgct ctggatcttc aacggaaacc tgttcagcgg
     1081 cctctggctg gcgatgatcg gtttcctcgt caacagctcg gcccgggcgg ccagccgcag
     1141 cacggtcgga gacaagctcg aagggatcac cgtctcgcag gtgatggaca ccgaaccggt
     1201 ttcgatcccg gccgagatcg actgtgccag ggccctcgag gagtacttcc tccgcttcgg
     1261 ttggtcctgg ttcccggtgg tcgaccggca cggctcgttc ctcggaatgg cggtcaggga
     1321 ccggcttgaa gcggtacccg aggtcgaaag ggacgcgacc ccggtgatct cgctggtcga
     1381 gatcaatccg ggcttttcgg tgcgctccga cgccggcctc gaccggctgc tcgggaaccc
     1441 ccagctacgc cgtttcgggg ccttgatggt gaccgacgac caggggaaac tggccggcgt
     1501 ggtgactgtc gagcagctcg gccgggctct tcaggcctga tttacgacct cgccgccttc
     1561 aaccggcgca ctacatagac tcggtatctg gcactggcag tcgcaacatt tctgatcgtc
     1621 ctcgccctga tcgcgagcga acgggtccac aggaccaagg tggccctgct cggtgccgcg
     1681 atcgtggtgc tcttcgttgg tcagtacaac gaggaactgg cgatcgaatc cgtcgagttc
     1741 gccacgcttg gtctgctcgc cggaatgatg atcctggtct acctgaccca gcagagcggg
     1801 gtttacgact acatcgcagt caaggccggc cagctttcgc gcggccggcc attcctgctg
     1861 gtggtgatgc tggtcaccac cgtggcggtg atgtcgggtt tcctcgacaa cctcacgacc
     1921 atccttctga tcgtgcccat caccttcctg ctggccgaca ccctggacat caaccccatg
     1981 ccactgatcc tgatcgaggt gatggccgcg aacatcggcg gtgctgcgac cctgatcggt
     2041 gacccgccga acatcatgat cggcaactcg gcaaacctga gcttcaacga cttcctgatc
     2101 aacaacatgc ccggcgtcgt ggtgatcctc gctaccgtga ccgtgggcct ctacttcgct
     2161 taccggagcc agctccaggt ggccgaggag aaccggaagt acgtccagga actggacgca
     2221 gccgcatcga tcagggacca cgacgagctc cggcgcaccc ttccggtcct gatcgggacg
     2281 atcatccttt tcttcctcca ccagtacatc cacgtcgagc cggcgacgat cgccctgacc
     2341 ggagcggcag tcggactgat ggtcacgaaa acaccaatcg aggaggccct ggagaagatc
     2401 gagtggacga cgctcttctt cttcatcggg ctcttcgtga tggttggtgc cctggaggtg
     2461 acaggcgcga tcgagaagct ggccgaggga atcaccagcg tcaccaacgg aaaccgggac
     2521 gccgaactgg tcgggatcat ctggacctca tcactggtcg gcgggatcgt cgacaacatc
     2581 cccctgaccg ccgcgatgat cccggtggtc gaagacatca aggctggcag caccgatcac
     2641 gcatactggt gggcgctctc gcttggcgcc tgcttcggag gcaacctgac cctgatctcg
     2701 gccgcctgca acgtcgccgc atcggggatg gcggagaagg cagggaggcc gatcgggttc
     2761 tggacttttc tcaaggtcgg cttcccggtg accgtggcgt cgaccgtcat ggcaaccgcc
     2821 tacgtgttgc tgaggttctg accgatgaaa ccggctagtt tctgcgagat gggcaaatgg
     2881 cagaccacat agtccgttcg gtcatccggg aggtcaagcc gctcaagacc agcgacccgg
     2941 tcgggtacgc ggcgcggctg gtgatcgagt ccggcctgcc ggctcttccg gtggtcgatg
     3001 agcacggcaa gtacgccggg ctcttcggcg agcgggagtt catgaccgca gtcttccccg
     3061 gatacgtggg aaccctcatg agcgcccgca tgatccggcg ctcactcgac gaaacgatcg
     3121 agcgacggat cggaagtcag gaggaaccgg tgcgcggata cctgaccacg gacccggtcc
     3181 tggtcgagga cgattacgcc gacacgcagc tggcggaaac cttcctccat caccgggtga
     3241 tggtggtccc gatcgcaacc gaaggccggg tccatgcagt cgtacccaga agcgagttct
     3301 tccaggcgct ctacgagaga atcgccgaca tcgccgaaga cttcggcgac tagagccctg
     3361 tcaggttcgg gagcccgggg cgactctacg caaatgccct ggaacgcaga aaaaccggcc
     3421 gaccagatgg acgggaactg gctgatcacc gagggagacc cggagagcgc ggtcatcctc
     3481 cacgtccccc atgcctcgag ggccttcccg accagggaac tcaacaggct ggtggtcgac
     3541 cgggagggcc tggacgcaga gctggacgcg atcaccgacg ccgacaccga acgactcgcc
     3601 gcagaagccg ccggactcgc cgaacttcgt ccctggatat tcgagaaccg ctactcccgg
     3661 ttgctggtcg atcccgaacg ttttcctgat gatcgcgagg agatgctgga ggccggtatg
     3721 ggccccgtct atctcaagac gacgaccggg gcggagtttc gccgcgcaga cgaggccgat
     3781 gatgaacgtc tcatgtccga gtactaccgg ccgtatgcga gcgccttcga gcggctggtt
     3841 gaacagaggc tggagcgtct tgggcgcgtg ctgctgattg acgtgcactc gtttccccga
     3901 gatccgctcc cttacgaact tcactccggc gaggcaaggc ctgcgctctg catcggcacc
     3961 gacaagttcc atacgcccca gtggctggtc gacgccatct ccggtctctg gccggacacg
     4021 gtcgaactgg accagccctt cagaggctgt tacgtgccac tctcgtttct ggagtcggaa
     4081 tcacgaatcg ccgccgtgat gctcgagttg aggcgtgacg tggtcgcccg ctgggccggc
     4141 ccggacagcc cggggcgaag gggcgaaccg ggcctgccgg ggttcgtggc cgcggtagca
     4201 gacctgtttt ccaggtacga cccgaccggc tagagccagt cgttcttgac caggagctcg
     4261 gcgacctgga cggcgttggt ggctgccccc ttgcggaggt tgtcgccggc cagccagagg
     4321 ttcagggtct tttcctggcc ggggtcacgg cggatccggc cgaccagggt ctcgtcgcgg
     4381 ccggcggcat cgagagcggt tggatacttg ccggcacccg gattgtcgac cacgatcacg
     4441 ttctcggctg cgccgaggat ctcgcgggca cggtccgggc tcaggtcttc gcgggtctcg
     4501 atgttgagtg actccgaatg gccggtgaag accggcacgc gggcgcaggt agcggagatc
     4561 ttcacctcgt caccgagacc gaggatcttc cgggtctcgg ccatcacctt gcgctcttcg
     4621 gtcgtgtaat cgtcgccgtc cttgaaggtc tcgacctgcg ggatgacgtt gaaggcgatc
     4681 tggtgggggt agacggcagg gtaagagacc gtctcgccgg ccagcacctg cccggcctgc
     4741 tcggtcagtt cggcgattgc cttctgaccg gtacccgaaa ccgcctgata gctcgaaagg
     4801 atcaggcgct cgaggcgcgc ctcacggtga atcggagcga gcacgagcat catcaccatg
     4861 gtggtgcagt tcgggttggc gatgatcccc ttgtggtccc tggcggcctc cgggttgacc
     4921 tcggccacca ccagcgggac gtcttcatgc atgcgccagt aagaggtgtt gtcaaccacc
     4981 accgcgccgg cttcagagaa cttcggagcc cattcggcac tcacggatcc gcccgccgag
     5041 gagatcagga tgtcgatgcc ctgaatcgac tcgtcgtcag gaaccacgca ggtcagttct
     5101 ccctcgccaa agggaacctt cttgccggcc gagcgttcgg aggcgaaagc caccacctcg
     5161 ctggccggga atgaacgctc gtgaaggatc tcgaggatgg tcgagccgac cgctccggtg
     5221 gcgccgagga cgccgaccct gtacctggat tcatccatga tcaggacggg gttacgggac
     5281 gatgttcgcc agtcggcgct tcggccagga tctgctcctc gccgaggtcg aaggcggcgt
     5341 ggagggaccg caccgcttcc ggcacctgat cggcctggat aacacatgaa atccggatcg
     5401 acgaggtgga gatcatctcg atgttgatct ccttttcgcc cagaacctcg aaaaccttgg
     5461 ctgcaacgcc ggggtggctg cgcattccgg cgccgacgat ggacaccttg ccgatctgct
     5521 cgtcggtgcg gacttcctgg ctgatgtcgc ccaggctggc gatcgcgtcg gtggccgtgg
     5581 ccagatcacc ggtctcgacc gtgaacgaga ggtccgcgag agcaccctcg tgcaccggct
     5641 cgttctggat gatcacgtcc acgttgacgt tcgcatcggc tagcgcgccg aagatacggc
     5701 cggcaattcc gggctcgtcc tttactccga tgacggccac gcgggcttcg tcggtggagt
     5761 gggtgacggc tgtgatgatc ggtcgttcca tggtttcgct ttcgggaagt acgagggtac
     5821 cggggaggtc ctcgaaactg cttcggcagt ggatcgggat gttgtagttg cgggcgtact
     5881 cgaccgaacg gagctggagt actccggcgc cggaggcggc catgtcgagc atttcctcga
     5941 aggagacgac aggaagtttt ctcgcgtcgg ggacgatgcg ggggtcagcg ctgaagacgc
     6001 cggtcacgtc ggtgtagatc tcgcaaactg acgcaccgag ggcggccgcg agcgcgactg
     6061 cggtcgtgtc tgacccaccg cgtccgagtg tggtcacgtc ccgactgtcg gttgatacac
     6121 cctggaatcc ggcgaccaga acgatcttgt cttcccccag cccctcacgg atgcggtcgg
     6181 ccttgacgtc gatgatcttg gccttcgtat gggaggagtc ggtcacgatt ccggcctgcg
     6241 agccggtgag tgagaccgcc ttgtgcccca tgtcgttgat cgccatcgcg gccagggcgc
     6301 aggaaattcg ctcaccggtc gagagcagca tgtccatctc gcggggatcg ggacgatcgg
     6361 agatctcgta ggccgaggcg accagttcat cggtcgtctt gccccgggcg gatagcaccg
     6421 cgacgacgct gttgccggct tcgcgggcag cgaccattcg ccttgcggca cgcttgatct
     6481 cttccgggcc ggcgaccgag gtcccgccga acttcattac gacgatgtca tctctttcag
     6541 gcatcacccc tgagcatacg gatcaccggt taaccgggtc cccaagcgca tttcaatatc
     6601 ctgcgccggg tgaccgacgg atctctccgc tgcccggaat gcggcagcga acatacctat
     6661 gaacttggcg gtttttccgt ctgctcgctt tgcgcccacg agtgggatcc ggctcacgac
     6721 cgcaccgaga tcgccgggga ttcggccccc gagattcgtg actcggtcgg gaacgtgctc
     6781 accgacggtg acacggtgac tgtgatcaag aacctgaagg tcaagggggc ttcggggccg
     6841 atcaaggcgg ggaccaaggt gaagggaatc cggctggtca acggtgtcgg cgaccatgac
     6901 atcgactgcc ggatcgatgg tttcgggcag atgcagctga agtccagcgt ggtcaagaag
     6961 gcctgagaat gccgaccggg ttcgagcagg acacggcggc cgagccgctg ggcgagggtc
     7021 gctacgcgat caacctgagt gaccgct
//
LOCUS       DAOQFI010000011         6344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_4591_length_6344_cov_2.832247, whole genome shotgun sequence.
ACCESSION   DAOQFI010000011 DAOQFI010000000
VERSION     DAOQFI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4591_length_6344_cov_2.832247"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /locus_tag="PLE93_00450"
     CDS             complement(<1..1979)
                     /locus_tag="PLE93_00450"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNH85544.1"
                     /translation="MSEETKDTKPLLPGIASPADLKGLSDEQLQRVADETRSYIIDVI
                     GEIGGHFGANLGTCEIAVALHSLLESPMDKILWDVGHQAYPHKVLTGRRDQLSTIRQY
                     EGLAPFCSIPESEHDIMGAGHASTSISYGVGLKEAMRKGIGEDGKVVAVIGDGALTGG
                     VAYEAMHAAGGYQTPMVILLNDNGMSISPNVGALSSYFQRARLKPGLFHAREEVEDRL
                     TRLPLGLGKRIERLGPELKSAIKAYWAPGLLFEELDLAYMGVIDGHDVGAMRRALTEA
                     FAADRPVVVHVHTVKGRGFAPAEQGGQAGQEKWHAAKPGSIVDGAPADSKPKLVPVQE
                     SDAPASIAPEILEMPEPKAAPPQYTQVFADALVSEAARDPRVIGITAAMAGGAGLQKL
                     ADERPDQYYDVGIAEQNAVLMASGIALQGGKPVCGIYSTFLQRAYDQIVHDVCLQELD
                     VTFAMDRAGLVGDDGPTHHGAFDIAYFRSLPNVVAMAPRDEAMLVHMLHTAIEHDGPA
                     ALRYPRGAAEGVEIPGGPELIPVGTGETLRNGERVALVGYGSGVGVALGAADLLDGEG
                     IRPTVADARFVKPLDTELMERLAVSHDLIVTIEEGVLQGGFGSAVIEHLEDNFANPGE
                     RARVLRVGLPDRFVTHGKPELLHREVGFTPEAVAE"
     gene            complement(1976..2854)
                     /locus_tag="PLE93_00455"
     CDS             complement(1976..2854)
                     /locus_tag="PLE93_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH85545.1"
                     /translation="MSYPEELRELVDSHLETIHFSRGDGVDGAETTGLDEAMRYSLLA
                     GGKRIRPVLCLATARAIGQPTDDFLGAAAALEMIHTYSLVHDDLPAMDDDDLRRGKPT
                     SHVKYGEAVAILAGDGLFAEAVGELARSPATPERVLEAVSLLSAATGVEGMVGGQYID
                     VSAQALDADGLRHLHSLKTGRLIRASIEVALALGGVTEASREPFRRFATQIGLLFQIV
                     DDILDVTGSDEELGKPQGSDERHGKVTYVSLYGLDRARSLAAETHEGALAALAGAGGE
                     IEDLKRIADYIYSREQ"
     gene            complement(2891..3064)
                     /locus_tag="PLE93_00460"
     CDS             complement(2891..3064)
                     /locus_tag="PLE93_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85546.1"
                     /translation="MSRLEEIRDRLDEITLALRDESVSDTDAAGLADEAAKLTAEAAS
                     EAAAAVERADRQA"
     gene            complement(3061..3558)
                     /gene="nusB"
                     /locus_tag="PLE93_00465"
     CDS             complement(3061..3558)
                     /gene="nusB"
                     /locus_tag="PLE93_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNH85547.1"
                     /translation="MRRSNQRRDAVFACYQQEVTDRPLSVLLAEAKPFTVELAEGVEA
                     HREDLDEVISEYAQGWDLNRIAPLEKNIMRVALYEMNFRDDVPFEVAIDEAVETAKKF
                     CGAEAPGFVNGILGAAARDMKADTEATNAGIETADTEATNAGTEEAGAEAVNTNTEAA
                     NGEAA"
     gene            3802..4797
                     /locus_tag="PLE93_00470"
     CDS             3802..4797
                     /locus_tag="PLE93_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85548.1"
                     /translation="MTHLEVTNLISSLAARQDGVVNRRQLLAAGVAASQVDSRVRNRT
                     LKKLYAGVYAVGHDRLSPLGRQRAAVMAGGPNAVLSHRAAAGLHGLLPPNGILEVIRS
                     SSPDPHQPPPAHASRAIHPGLVIHRTRSLKRGEVRNRHGIPVTSAVRTVINLAEREPP
                     RSLDTAIRRGIARNLFDLDELNRAIDRARGRKGIAKLRKVLANWNPGKLRTKSGLENK
                     TADRFRGWGIPDPLINDFRCGHEVDFQWPGSNILVESDGGAYHASRADRHRDYQKMLD
                     LQAAGNVVVRIDEDMVEKEAYEVGLKLRKLFAEEGVVELQPISPQPVAVSDRAPP"
     gene            complement(4746..5180)
                     /locus_tag="PLE93_00475"
     CDS             complement(4746..5180)
                     /locus_tag="PLE93_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNH85549.1"
                     /translation="MEMFQRDLTLEARSRGFHPVTDEIERGLPELGTLRIGLIHLLIR
                     HTSASLTLNENASPDVRRDFSSWIDSVVPERPTAYRWTHTLEGADDMPAHIKGSVTGF
                     DLTIPVRDGCFHLGAWQGIYLCEHRDHGGARSLTATGWGEIG"
     gene            complement(5401..5961)
                     /gene="efp"
                     /locus_tag="PLE93_00480"
     CDS             complement(5401..5961)
                     /gene="efp"
                     /locus_tag="PLE93_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNH85550.1"
                     /translation="MISTSDFRNGSHIEVEGKIMRIIEFQHVKPGKGGAFVRTKLRRI
                     EDGGVIDKTFRAGEKFRPVRTESKRMQYLYESGDAVVFMDNQSYDQIEIPADIAADAM
                     RWIQPNDEVEVLFVDENPSDVSVGSSIEAKVVKTEPGLKGDTASGGGTKPATLESGVE
                     VQVPLFINEGEVIKVNTETAEYMSRA"
     gene            6021..>6344
                     /locus_tag="PLE93_00485"
     CDS             6021..>6344
                     /locus_tag="PLE93_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HNH85551.1"
                     /translation="MTTTPALLDELLRTPGPSGYEAPAADVWRKAAGFAEITTDGLGS
                     SAATIPSSDENDEAPLVAVVGHIDEIGLVITHIDEKGFLYFAPIGGWDPQILVGQRVT
                     ITGNEG"
BASE COUNT         1043 a         2104 c         2041 g         1156 t
ORIGIN      
        1 ttctcggcca ccgcctccgg ggtgaagccg acttcccggt ggagcagttc gggcttgccg
       61 tgggtcacga aacggtccgg caagccgacc cgcagcaccc gcgcgcgctc gcccggattg
      121 gcgaagttgt cttccagatg ctcgatcacg gccgagccga aaccgccctg gagaacccct
      181 tcctcgatcg tcacgatcag gtcgtggctg accgccagac gctccatgag ttcggtgtcg
      241 agcggcttga cgaagcgggc gtcggccacg gtcggccgga tcccctctcc gtctagaagg
      301 tccgccgctc cgagcgccac tccgaccccg ctgccgtagc cgaccagggc gacccgctcc
      361 ccgtttcgga gggtttcgcc ggtgcctact gggatcaact ccgggccgcc cggaatttcc
      421 actccttcgg cggccccgcg cggatagcgc agcgcggccg gcccgtcgtg ttcgatcgcc
      481 gtgtgaagca tgtggaccag catcgcctcg tcgcgcgggg ccatcgccac cacgttcggg
      541 agcgagcgga agtaggcgat gtcgaaggca ccatggtggg tcggcccgtc atccccgacc
      601 agcccggccc ggtccatcgc gaaggtcacg tcgagctcct gcaggcagac gtcgtggacg
      661 atctggtcgt aggcccgctg caggaaggtc gagtagatgc cgcagaccgg cttgccgccc
      721 tggagcgcga tgccgctggc catcagaacc gcgttctgct cggcgattcc gacgtcgtag
      781 tactggtcgg gccgttcgtc tgcgagcttc tggaggccgg ccccgccagc catcgcggcg
      841 gtgatgccga ttacccgcgg gtcccgtgcc gcctcgctga ccagagcatc ggcgaagacc
      901 tgtgtgtact gcggcggagc agccttcggc tcgggcatct cgaggatctc gggagcgatc
      961 gaggccgggg catcggattc ctgcaccggg accagcttcg gcttcgagtc ggccggagcc
     1021 ccgtcgacga tcgagccggg cttggccgca tgccatttct cctggccggc ctgaccgccc
     1081 tgctcggccg gggcgaaacc gcggcccttg accgtgtgga cgtggacgac caccggccgg
     1141 tccgcggcga aagcttcggt cagagcccgg cgcatcgccc ccacgtcgtg gccgtcgatc
     1201 acacccatgt aggcgagatc gagttcttcg aagagcaggc cgggggccca gtaggccttg
     1261 atcgccgact tgagttccgg cccgagcctt tcgatccgct tgccgaggcc gagcggcagc
     1321 ctggtcaggc gatcctcgac ctcttcgcgg gcatggaaga gcccgggctt cagccgggcc
     1381 ctctggaagt agctggaaag cgccccgacg tttggcgaga tcgacatgcc attgtcgttg
     1441 agcagaatca ccatcggggt ttggtagcca cccgcagcat gcatcgcctc gtaggcgacg
     1501 ccaccggtca gggcgccatc accgatcacc gcgacgacct tgccgtcctc gccgatcccc
     1561 tttcgcatcg cttccttgag gccgaccccg taggagatcg aggtcgaagc gtgaccggca
     1621 cccatgatgt cgtgctcgga ttccggaatc gagcagaacg gagcgaggcc ttcgtactgg
     1681 cggatcgtcg agagctggtc gcgccggccg gtcagcacct tgtgcggata ggcctgatgg
     1741 ccgacgtccc agaggatctt gtccatgggt gattcgagca ggctgtgcag ggcgaccgca
     1801 atctcacagg ttcccaggtt ggccccgaag tgaccgccga tttcaccgat cacatcgatg
     1861 atgtaggagc gggtctcatc ggcgacccgc tggagctgct cgtcgctgag acccttgagg
     1921 tcggccggag aggcgatccc gggaagcaga ggcttcgtgt ctttggtctc ttcgctcatt
     1981 gttctctcga ataaatgtag tcggcgatgc gtttcaagtc ttcgatttcg ccgccggcac
     2041 cggccagcgc ggccagggcc ccctcgtggg tctccgcggc cagcgaccgt gcccggtcca
     2101 atccatacag gctgacataa gtcacctttc cgtgacgctc gtcgctgccc tggggcttcc
     2161 cgagttcttc gtcgcttccg gtcacgtcga ggatgtcgtc gacgatctgg aagagcaggc
     2221 cgatctgggt ggcgaagcgc cggaacggct cacggctcgc ttccgtgacc ccgccgagcg
     2281 ccagcgccac ttcgatgctg gcccggatga gccggccggt cttcagcgaa tggaggtgcc
     2341 tgagcccgtc cgcgtccagg gcctgggccg agacgtcgat gtactggcca ccgaccattc
     2401 cttccacccc ggtcgcggcg ctgagcaggc tgaccgcttc aagaacccgt tccggggttg
     2461 ccggagagcg ggccagttcg ccgaccgctt cggcgaagag cccatcgccc gccaggatcg
     2521 cgaccgcctc gccgtatttc acatgtgagg tcggcttgcc gcgacgcagg tcatcgtcgt
     2581 ccatggccgg caggtcgtcg tggaccagcg aataggtgtg gatcatctcc agggcggccg
     2641 cggccccgag gaaatcgtcg gtcggctggc cgatcgccct ggcggtagcc aggcagagca
     2701 ccggcctgat gcgcttgcct ccggccagga gcgagtaacg catggcctcg tcgagccccg
     2761 tcgtctcggc cccgtcgacg ccatcgccac ggctgaagtg aatcgtttcg aggtgactgt
     2821 cgaccagctc acgcagttct tcggggtagc tcaagagcgg cttccgccct tcaatggaca
     2881 ttccacgggc tcaggcctgc cggtcggcgc gctcgaccgc tgccgcggct tcactcgcag
     2941 cttcggccgt gagcttcgcg gcttcgtcgg ccagacccgc cgcgtcggtg tcggaaacgc
     3001 tctcgtcacg cagcgccaga gtgatctcgt cgagccggtc gcgaatttcc tcgagtcgac
     3061 tcacgccgcc tccccattgg ctgcttcggt gttggtgttg accgcttcgg cgccggcttc
     3121 ttcggtgccg gcgttcgtcg cttcggtgtc ggctgtttcg atgccggcgt tcgtcgcttc
     3181 ggtgtcggcc ttcatgtccc gggcagcggc cccgaggatc ccgttgacga agccgggagc
     3241 ctcggctccg cagaatttct ttgcggtctc gaccgcttcg tcgattgcga cttcgaaggg
     3301 cacatcgtcc cggaagttca tctcgtagag ggcgacccgc atgatgttct tctcgagcgg
     3361 cgcgatccgg ttcaagtccc aaccctgggc gtattcggag atgacttcat cgagatcctc
     3421 ccgatgagct tcaacgccct cggccagctc gaccgtgaac ggtttcgctt cggcgagtag
     3481 gacggagagg ggccgatcgg tcacttcctg ctggtaacag gcaaaaaccg catcccgacg
     3541 ctgattggag cgacgcatca gccaagaata ttggttcgaa cacccaacct cccggtgggt
     3601 cggatttccg tcctccatgc ggactttccg acccgcctcc aggcccgcac cctcccccgt
     3661 cccgccggtg ggtcggattg tcggtcctga cgctggaaat ccgacccacc ggcgagagga
     3721 accgcgagtt tcccggggcc aggttgcaaa tcgttgcacc tgtagagcca cgtgtgagca
     3781 tttccccggc acatttgggg gatgacgcat ctggaggtga ccaacctgat ctcttcgctc
     3841 gctgcccggc aggacggagt cgtcaaccgc cggcagctcc ttgcggccgg ggtggcggcc
     3901 agccaggtcg attccagagt caggaaccgg accctgaaga agctctacgc cggcgtttac
     3961 gcagtcggcc acgaccggct ctcgcccctg ggcaggcagc gggccgccgt gatggccggt
     4021 ggtccgaatg cggtgctttc gcaccgtgcg gcggccggcc ttcacggcct gctgccgccg
     4081 aacgggatcc tcgaagtgat ccgcagctca agccccgacc ctcaccagcc gccgcccgcg
     4141 cacgcaagcc gggcaatcca ccccggtctg gtcatccacc ggacccggtc cctcaagcgc
     4201 ggcgaagtga ggaatcgcca cggcattccg gtcacatcag cggttcgaac cgtgatcaat
     4261 ctggctgaaa gggaaccgcc caggtcgctc gacaccgcca tcaggagagg cattgccaga
     4321 aacctctttg accttgacga attgaaccgg gccattgacc gggcccgggg gcggaaaggc
     4381 atcgcgaaac taaggaaggt gctcgcgaac tggaacccgg gcaagctcag gaccaaatcg
     4441 ggtctggaga acaagacggc tgaccggttc cgcgggtggg gcattccgga tcccctcatc
     4501 aacgacttcc gctgcggtca cgaagtcgac tttcagtggc ccggatcgaa catcctggtc
     4561 gaatccgacg gtggcgccta tcacgcgagt cgggcggacc ggcatcggga ctatcagaag
     4621 atgctcgatc tccaggcggc aggaaacgtc gtcgtgcgga tcgatgagga catggtcgaa
     4681 aaggaggcct acgaagtggg gttgaaactg cgaaagctct tcgcggaaga aggcgtggtc
     4741 gagcttcagc cgatttcacc ccagccggtc gcggtcagcg accgggcgcc accgtgatcc
     4801 cggtgttcgc agaggtagat cccctgccag gctccaagat gaaagcaccc gtcccgcacc
     4861 ggaatcgtca agtcgaatcc ggtcactgaa cccttgatgt gagccggcat gtcgtcggcg
     4921 ccctcaaggg tgtgcgtcca gcgataggcg gtcggccgtt ccggcacgac cgagtcgatc
     4981 catgaactga agtcccgcct cacatcgggc gaggcgtttt cgttcagggt caatgaagcc
     5041 gacgtatgcc ggatcagcag gtggatcagg ccgatccgca gtgttccgag ctccgggagg
     5101 ccccgttcga tctcgtccgt gaccgggtga aagcccctcg atcgagcctc gagagtcaga
     5161 tctcgctgaa acatctccat cgcagaaccg tacccgcccg gaggtgggtc ggataaccag
     5221 ctgccaggac ggaaatccga cccacccctg cgggaggttc aagcggcggt gcctggtggg
     5281 tcgggattca ggtcctgatg acggaaatcc gacccacccg gtgggcaggc tttgggctgg
     5341 ggcccgggtt gggggcctgg gccgggttcg ggggtgtccg ggtccggggg tggccggggt
     5401 tcaggcgcgg gacatgtatt cggcagtctc ggtgttgacc ttgatcactt cgccctcgtt
     5461 gatgaaaagc ggcacctgga cctcgactcc cgactcaagg gtcgccggct tcgtgccgcc
     5521 gcccgaggcg gtgtcgccct tcaggccggg ttcggtcttc accactttcg cttcgatcga
     5581 gctgccgacc gagacatcgg aggggttctc gtccacgaat aggacttcga cttcatcgtt
     5641 gggctggatc cagcgcatcg cgtccgcagc gatatcggcc ggaatttcga tctggtcgta
     5701 gctctggttg tccatgaaga cgaccgcgtc accagactcg tagaggtact gcatccgctt
     5761 tgactcggtg cgaaccggcc ggaacttctc cccggcgcgg aaagtcttgt cgatgactcc
     5821 gccgtcttcg atgcggcgaa gcttggtgcg cacgaacgca ccgcccttgc cgggcttcac
     5881 atgctggaac tcgatgattc gcatgatctt gccttcgacc tcgatgtggg acccgttgcg
     5941 gaaatcgctg gttgaaatca cggcagacac tttagccaca gggcgcccgg gcgagtcgcg
     6001 ccgaacggca taatcaatcc atgactacga ctcccgcgct tctcgacgaa ttgctccgca
     6061 cccccggccc ttccggctac gaagctcccg ccgccgacgt ctggcgcaag gcggccggtt
     6121 tcgccgagat caccaccgac ggtctcggtt ccagcgcggc gacaatcccg agttcggacg
     6181 aaaatgacga agcgcccctc gtcgcggtgg tcggccacat cgacgagatc ggcctggtca
     6241 tcacccacat cgacgaaaag ggcttccttt acttcgcgcc gatcggcggc tgggatcccc
     6301 agatcctggt cgggcagcgc gtcacgatca ccggcaacga aggt
//
LOCUS       DAOQFI010000012         6337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_4599_length_6337_cov_3.669532, whole genome shotgun sequence.
ACCESSION   DAOQFI010000012 DAOQFI010000000
VERSION     DAOQFI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4599_length_6337_cov_3.669532"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..986)
                     /locus_tag="PLE93_00490"
     CDS             complement(138..986)
                     /locus_tag="PLE93_00490"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH85552.1"
                     /translation="MKAWRWTKDHMVLFVGLLALAYMLIPILVIAVFSFNNPEGRYNF
                     EWVGFTTEYWGKPFAITELTDALLVSIRLAFFTAIGATILGTLIAIALVRYQFFGRRA
                     ANLLIVIPMATPEVVIGASLLSMFLIYGAKLGFGTLLIAHIMFSISFVVIVVRSRLIG
                     FDRRLEEAAADLGANAFTTFRKVTLPLIAPGVISAALLSFALSIDDFVISNFNSGSTV
                     TFPLYIFGANQRGIPVQVNVIATMLFAVTVIAMIAVILQQRRAEKAAGLRPDEIADKK
                     AISLPV"
     gene            complement(983..1849)
                     /locus_tag="PLE93_00495"
     CDS             complement(983..1849)
                     /locus_tag="PLE93_00495"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH85553.1"
                     /translation="MTIRRRLIPYWLLAPGLIWLVLFFVIPMYYMGELSLKTGSLETG
                     YQLTWAFENYTDSISEYSTQFIRAFWYAGVATFFCLLIGYPLAYAIAFKAGRWKNLML
                     FAVVAPFFTTYLIRTIAWKTILDDSSPVVSFLKTIHLVPQDGRVLATSAAVIAGLTYN
                     FLPFMVLPLFTALERLDQRLLEAGKDLYASATQTFWRITLPLTMPGIIAGILLTFIPA
                     AGDYVNATFLGGTNQAMIGNVIQSKYLVVNDYPDAAALSFTLMAIILVALLFYIKFAG
                     SEALMGEEEETR"
     gene            complement(1916..3070)
                     /locus_tag="PLE93_00500"
     CDS             complement(1916..3070)
                     /locus_tag="PLE93_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNH85554.1"
                     /translation="MSESNVKRVRLLALAVLALAVSMVLVACGGGGIDEGSDNSDVTT
                     ATGGDPQGSLKISNWPLYIDGATIKDFQNDTGINTTYTEDVNDNNEFFGKVQQQLAQG
                     DSGGRDIIVVTDWMAEKMYNLGYIQNLDKAKLPTVEKNLIPSLQSPSFDPERKYSVPW
                     QSGMTGLVVRKDLAPDVKSINDLFDPKYKGKVTLLTEMRDTVPLVMAADGVDPKEATT
                     QDWLDAIDKIKENVDNGQIRRFTGNDFVQDLAKGDVVAAIGWSGDAVQAQSDNENIEY
                     VQPEQGCSIWSDNMLIPVGAPNPAAAYKFMNYVYEPENQAQITKYVNYVSPVKGVREI
                     LEKQDPAIANNNLIFPSEQFTKNCFAQVSPPGDEAAQQEVEQAFQDVVTG"
     gene            complement(3070..4194)
                     /locus_tag="PLE93_00505"
     CDS             complement(3070..4194)
                     /locus_tag="PLE93_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH85555.1"
                     /translation="MEGVTKKFGDFTAVSEMDLDIADGEFFTMLGPSGCGKTTTLRMV
                     AGFEQPTTGKVLIDGTDVAGQPAYKRPTNTVFQSYALFPHMTLEENVGFGLKRKKVPK
                     AEIKQRVANELERVGLSREAKRKPNQLSGGQQQRVALARALVNLPKVLLLDEPLGALD
                     LKLRKGLQVQLKQIQRDVGITFVYVTHDQEEALTMSDRIAVMNEGRIEQCADPETVYD
                     RPATTFVAGFIGVSNLMPAEVKGSNEIQVVNGPLVPTPDAAKYSGGEKCFAVVRPEKL
                     TVSLPENAPSDQPGVDGIVESSLYLGTSTQMAVRVTEDVMMTVLVPNASEDARQHLPG
                     GGAKVRLNWLPEHMHLVAESESATAATDPTPTEKVTEGVN"
     gene            4527..6008
                     /locus_tag="PLE93_00510"
     CDS             4527..6008
                     /locus_tag="PLE93_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85556.1"
                     /translation="MKTSADHKLPRLARCLALLAMLVALACFGGRADAAVTGDNPCPT
                     SASNSLSWMGASVGQDFTIPSLRHQADSSQVPATFPMTVLRPADTVTYPGPRPVVVFQ
                     HGLGGNRCALWWAAQYLAGHGFVTTVHRSLPGNEQTASDAYRSGVDAVRSVIAYLKGL
                     QVSDPNLGPMIDLGRLGLSGHSMGSIISSQLQGDPSLGIKAIVAFDTLRRYVQGDPGA
                     ARIDCQSDPVPATQVTPSVPALSFAMDEPCAYKPDFKPADLKLSGPRWWSEHGIPNMQ
                     LVMKGYPHGGFTDGASADKKLDLAHWVLPWFERWLNGDKSQEQLLAAPVVNGRPVADL
                     LSSEFLSSACLDGIADSEDLRSWFNAGSPATSGAGRCDSPPGQSPDPAHPAPALTNRK
                     LVKLVRLSSSRGWVRAGGKARLKLRITVKGMGQPVKNLNLLVSSSNRRVRVAGTRKVQ
                     RINPASTRVVVLTVRAGRKARNRVRVKVTVAGRTVGRTLRIRR"
     gene            complement(6005..>6337)
                     /locus_tag="PLE93_00515"
     CDS             complement(6005..>6337)
                     /locus_tag="PLE93_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85557.1"
                     /translation="YYRRELFKSVKNLLFVGLAPVIGALLLTYLFIKSLVDWSDPANS
                     YSGASWFGFAPPAVIGVGFLLLGAMLLLAWRIHQEEPFFKRRREVADPGLLEGKAGAA
                     VALAGPEN"
BASE COUNT         1129 a         1959 c         2056 g         1193 t
ORIGIN      
        1 aatgccgcgc gtgcgtccgt cccggctacc cccacccgac cccacggcgt aaccaccggc
       61 ccccgcggcg taaccacccg atcccacggc gtaaccaccg gggcccactc gggcccgcgg
      121 cgtggtcacc aacagggtca gacggggagg gagatggctt tcttgtcggc gatctcgtcc
      181 ggccgcaggc ccgctgcctt ctcggcccgg cgctgctgca ggatgacggc gatcatcgcg
      241 atcacggtga cggcgaagag catggtcgcg atcacgttga cctggaccgg gatgccgcgc
      301 tggttggcgc cgaagatgta gagcgggaag gtcacggtgg agcccgagtt gaagttcgag
      361 atcacgaagt cgtcgatcga gagggcgaag gaaagcaggg cggccgagat gacccctggc
      421 gcgatcagcg gcagggtcac cttgcgaaaa gtcgtgaagg cattcgcccc gaggtctgcc
      481 gccgcttcct cgagccgtcg gtcgaatccg atcagacgcg acctcaccac gatcaccacg
      541 aagctgatcg agaacatgat gtgggcgatc agcagcgtgc cgaacccgag cttggcgcca
      601 tagatcagga acatcgagag cagcgacgca ccgatcacga cctcgggggt cgccatcggg
      661 atcacgatca ggaggttcgc cgcccgacgc ccgaagaact ggtaccggac cagggcgatt
      721 gcgatcagag tgccgaggat cgtggcaccg attgcggtga agaaggcaag ccggatactg
      781 accagcagtg cgtcggtcag ttcggtgatc gcgaagggct tgccccagta ttcggtggtg
      841 aagccgaccc actcgaagtt gtaacggccc tccgggttgt tgaacgagaa gaccgcgatg
      901 acgaggatcg ggatcagcat gtacgcgagg gcaagcaacc cgacgaagag caccatgtga
      961 tccttggtcc agcgccaggc tttcatcgtg tctcctcctc ttcgcccatc aacgcttccg
     1021 aaccggcgaa ctttatgtag aacaggagtg cgacgaggat gatcgccatc agcgtgaagg
     1081 agagcgccgc cgcatccgga taatcgttga ccaccaggta cttcgactgg atcacgttgc
     1141 cgatcatcgc ctggttggtg ccgccgagaa aagtcgcgtt gacgtagtca ccggccgccg
     1201 ggatgaaagt caagaggatg ccggcgatga tgccgggcat ggtcagtgga agggtgatcc
     1261 gccagaacgt ctgcgtggct gacgcataca aatccttgcc ggcctcaagc agccgctggt
     1321 cgagccgctc gagagcggtg aagagcggca ggaccatgaa gggcaggaag ttgtaggtga
     1381 ggccggcgat caccgcagcc gatgtggcca ggacccgccc gtcctgaggc acgaggtgaa
     1441 tcgtcttgag gaagctgact accgggctgg agtcatcaag gatggtcttc caggcgatgg
     1501 tccggatcag gtaggtggtg aagaagggtg ccaccaccgc gaaaagcatc aggttcttcc
     1561 agcgccccgc cttgaaggcg atcgcgtaag cgagcgggta gccgatcaga aggcaaaaga
     1621 aagtcgcgac gccggcgtac cagaaggccc tgatgaactg ggtcgagtac tcgctgatcg
     1681 agtcggtgta gttctcgaac gcccaggtga gctggtatcc ggtttcgagc gagccggtct
     1741 tcagcgagag ctcccccatg taatacatgg ggatcacgaa gaagagaacg agccagatca
     1801 ggcccggggc gagcagccag taaggaatca gacgccggcg gatcgtcatc agttggaact
     1861 ctttctcatt gatccgccgg cttcttcatc ctgatttctg tgctgtttcg tggggtcagc
     1921 ctgtgaccac gtcctggaag gcctgctcga cttcctgctg cgcggcttcg tcgccgggcg
     1981 gtgagacctg cgcgaagcag ttcttggtga actgctcact ggggaagatc aggttgttgt
     2041 tggcgatcgc aggatcctgc ttctcaagga tctcccttac gcccttgacc ggacttacgt
     2101 agttcacgta cttggtgatc tgcgcctggt tttcgggctc atagacgtag ttcatgaact
     2161 tgtaggccgc ggccgggttg ggcgccccaa ccgggatcag catgttgtcg gaccagatcg
     2221 aacagccctg ctcgggctgg acgtactcga tgttctcgtt gtcggactgg gcctggaccg
     2281 cgtcaccgga ccagccgatc gccgcgacca catcgccctt ggccaggtcc tggacgaagt
     2341 cgttgccggt gaagcggcgg atctgcccgt tgtcgacgtt ctccttgatc ttgtcgatcg
     2401 cgtcgagcca gtcctgggta gtcgcctcct tggggtcgac cccgtcggcg gccatgacca
     2461 ggggcaccgt atcccgcatc tcggtcagca gggtgacttt gcccttgtac ttcgggtcga
     2521 agaggtcatt gatcgatttc acgtccggag cgagatcctt gcggacgacc aggccggtca
     2581 tgccggactg ccacggcacg ctgtacttgc gttcggggtc gaacgacgga ctctggaggc
     2641 tcgggatcag gttcttctcg acggtgggaa gcttggcctt gtcgagattc tggatataac
     2701 cgaggttgta catcttctcg gccatccagt cggtcacgac gatgatgtcg cggcctcccg
     2761 agtcgccctg tgcgagctgc tgctggacct tgccgaagaa ctcgttgttg tcgttgacat
     2821 cctctgtgta agtcgtgttg atgccggtgt cattctggaa atccttgatc gtggccccgt
     2881 cgatgtagag gggccagttc gaaatcttca gtgatccctg cggatcgccg ccggttgcgg
     2941 tggtcacgtc agagttgtca gagccttcgt cgatgcctcc gccgccgcag gcgacgagga
     3001 ccatcgagac ggccagggcc aggacggcca gggccagcag tcgaacgcgt ttcacattgc
     3061 tctcggacat cagttgactc cttcggtgac tttttcggtg ggcgttgggt cggtggcggc
     3121 cgtggccgat tcggactcgg ccacgaggtg catgtgctcg ggcagccagt tgaggcgaac
     3181 ctttgcgccg ccgcccggga ggtgctgccg ggcatcttcg gacgcgttcg ggacaagcac
     3241 ggtcatcatc acgtcctcgg tgacccgcac ggccatctgg gtggaggtgc cgaggtaaag
     3301 cgagctttcg acgattccgt cgacgccggg ctggtcggat ggcgcgttct cagggagcga
     3361 aaccgtcagc ttctccggac gcactaccgc aaaacacttc tcgccaccgg agtacttcgc
     3421 ggcgtcgggg gtcgggacca aggggccgtt cacgacctgg atttcgttcg atcccttgac
     3481 ttcggcgggc atcaggttgg aaacgccaat gaagcccgcc acgaaggttg tggccggtcg
     3541 gtcgtagacc gtctccggat cggcgcattg ttcgatgcgc ccctcgttca tgaccgcgat
     3601 ccggtcactc atggtcaggg cttcttcctg gtcatgggtg acgtagacga aggtgatgcc
     3661 gacgtcgcgc tggatctgct tgagctggac ctggaggcct ttgcggagtt tgaggtcgag
     3721 agcgccaagc ggctcgtcga gcaagagcac cttgggcagg ttgaccaggg cgcgggccag
     3781 ggcgacgcgc tgctgctggc cgccagagag ctgattcggt ttgcgcttcg cctcgcggga
     3841 aaggccgacc ctctcgagtt cgttcgctac acgctgcttg atttcggcct tgggcacctt
     3901 cttgcgcttg agaccgaacc cgacgttctc ctccagggtc atgtggggaa aaagcgcgta
     3961 ggactggaag acggtgttgg tcgggcgctt gtaggccggt tggccggcga catccgtgcc
     4021 atcgatcagg accttgcctg tcgtcggctg ttcgaagccg gcgaccatcc gcagggtggt
     4081 cgtcttgccg cagccggagg gaccgagcat ggtgaagaac tcgccgtcgg cgatgtcgag
     4141 atccatctca cttactgccg tgaaatcacc gaactttttg gtgactccct ccaagcgaac
     4201 tcccccgctc ccagatctcg cggcgttccc ggctacggcc tcggtctgat ccgcatccgg
     4261 atcgatcggc ggtagatctg gcgagccttc catcgaaccc ctctctcagc tggacaagaa
     4321 ccggagtttc agaacgcctc ctccggcaag ttcggttgaa ccgtatgcga aaacgggtgg
     4381 cctccgcaac cacgttttgt ccaaacggga catatcgaat gtgcccattg tccatatccg
     4441 cggcttcgcg actgcctcta tgcacggctt tcgcaggcgc gaccggatcg caactcgcgg
     4501 cggtctcggt gttgggatta gtacccgtga aaacctctgc cgatcacaag cttccccgcc
     4561 tggcgagatg cctcgccctg ctggccatgc tcgtcgccct tgcctgtttc ggcggccggg
     4621 ctgacgctgc ggtgaccggt gacaatccct gcccgacttc tgcctcgaat tcgctttcgt
     4681 ggatgggagc ttcggtcggg caggacttca cgatcccctc gcttcgccat caggccgatt
     4741 ccagccaggt tccggcgact ttcccgatga ccgtgctccg ccccgccgac acggtgacct
     4801 atcccgggcc ccgcccggta gtcgtctttc agcacgggct cggtggcaac cgctgtgcgc
     4861 tctggtgggc ggcccagtac ctggccggtc acggcttcgt gaccacggtt caccgctcgt
     4921 tgccgggtaa cgagcagacc gcttccgatg cctaccgcag cggtgtcgac gcagtccgct
     4981 cggtgatcgc gtacctcaaa ggcctccagg tctcggaccc gaacctcggg ccgatgatcg
     5041 acctgggccg gcttggactt tccgggcatt cgatgggatc gatcatttcg agccagctcc
     5101 agggggatcc ctccctgggc atcaaggcga tagtcgcctt tgacaccctc cgccgatacg
     5161 tgcaggggga tcccggcgcg gcccggatcg actgccagag tgatcccgtc ccggccaccc
     5221 aggtcacccc gagcgttccg gcgctcagct tcgcaatgga cgagccctgc gcctacaagc
     5281 cggacttcaa accggccgac ctgaaactga gcggcccccg ttggtggtcc gaacacggga
     5341 ttccgaacat gcagctggtc atgaaggggt acccgcacgg cgggttcacc gatggcgcct
     5401 cggccgacaa gaaacttgat ctggcccact gggttctgcc ctggttcgaa cgctggctga
     5461 acggtgacaa gagccaggaa cagctcctcg ccgccccggt tgtcaacggc aggccggtcg
     5521 ccgacctgct cagcagcgag ttcctttctt ccgcctgtct ggacgggatc gcggacagcg
     5581 aggatctgcg gagctggttc aacgccggct cgccggccac gagcggagcc ggtcgctgcg
     5641 attcgccgcc cggccagtca ccggatcccg cccatcccgc tcccgccctc accaatcgca
     5701 agctggtcaa gctggtcagg ctgagttcat cccgcggctg ggtccgggcc ggcggcaagg
     5761 ccagattgaa actgcggatc accgtcaagg gcatgggcca gccggtgaag aacctgaacc
     5821 tgctggtcag ctcctcgaac aggcgcgtgc gcgtcgccgg cacgaggaaa gttcagcgga
     5881 tcaatccggc cagcacccgt gtggtcgtgc tgaccgtgag ggctggaagg aaagcccgaa
     5941 acagggtccg ggtcaaggtg accgtggccg gcagaaccgt cggcaggacg ctgcggatcc
     6001 ggcgctagtt ttccggtccg gccagggcga ccgccgctcc ggccttgccc tccagcagcc
     6061 ccgggtcggc gacctcccgg cgccgcttga agaacggctc ttcctgatgg atccgccagg
     6121 ccagcaggag catcgcgccg agcagcagga agccgacccc gatcaccgcc ggcggggcga
     6181 agccgaacca ggaggcgccg ctgtaggaat tggccggatc ggaccagtcg accagcgact
     6241 tgatgaagag gtaggtgagc agcagggctc cgatcaccgg ggcgaggccg acgaagagca
     6301 ggttcttcac cgacttgaag agctcccggc ggtagta
//
LOCUS       DAOQFI010000013         6322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_4622_length_6322_cov_3.222595, whole genome shotgun sequence.
ACCESSION   DAOQFI010000013 DAOQFI010000000
VERSION     DAOQFI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4622_length_6322_cov_3.222595"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1183
                     /locus_tag="PLE93_00520"
     CDS             158..1183
                     /locus_tag="PLE93_00520"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNH85558.1"
                     /translation="MKIGVLTGGGDCPGLNAVIRAVVLRGTREWGHEFTGYLDGWKGP
                     LENESIELGVGDVRGILAQGGTILGSSRTNPEAIEGGVDQVIDNVRAAGLDGLIAIGG
                     DDTLGVARILTESGLGVVGVPKTIDNDLNATDYTFGFDTAVNIVMESLDRLHTTATSH
                     HRCLVVEVMGRHAGWIALHGGMAGGADQILVPEEEFDLEQVCRQVNRSFEAGVAPIVV
                     ASEGAIPAEGSELTTAEGTDAFGHVRLGGIGGWLAERIEENTGHETRSVVLGHLQRGG
                     TPTAFDRVLATRLGSRAIQAANEGEWGTMTTLRGTSIELAPLADAVDESKVVPPERLR
                     EAQALFD"
     gene            complement(1238..2173)
                     /locus_tag="PLE93_00525"
     CDS             complement(1238..2173)
                     /locus_tag="PLE93_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNH85559.1"
                     /translation="MSDPNSKRPERILVTGGTGLIGSTLCNALIDRGDSVVGLSRDPE
                     RASRSLPRVEWHGWEGERERPPRDAFEGVDAVINLAGEPINQRWNDEVKDRIARSRIK
                     ATKNLVAKIAALDQRPKTLISASAIGYYGDRGDELLYEDAEPGDDFLADIVVRWEEAA
                     RSAEDHGLRVATIRSGHVLDPRGGLLKELMLPFKAGVGGPIAGGKQYMSWVHRHDEIG
                     ILLWALENESVSGPVNATAPNPVTNKEFSKALGKQLGRPSFLPVPGFAIDIVKGGGVG
                     HAAREGQRVFPKKATDGGYEFKQPELAGALANLLD"
     gene            2246..4423
                     /locus_tag="PLE93_00530"
     CDS             2246..4423
                     /locus_tag="PLE93_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNH85560.1"
                     /translation="MRLVTSNRSRTWTVAAATAALALFGILSGPGPAGAAPARCFGQK
                     VDRVITGSNKTVNVGAREVVFVEGNRITVKATAFSRICAGDGSQTIEAGHGGPGNDRI
                     SAAKGDSTVFGGPGDDQIETEKGNNIEVQGGPGDDEIRPGKGNNVKVDGDEGNDRIFL
                     FTQTAKGVVDGGLGNDRIEGSQSHDKLIGGPEKNPRNLPDTDLIIGEGGNDSIFDYGG
                     SGNRLIGLEGADELSSLGDAVSELHGGNGSDKLFSNGGRTAAGVTEQLFGEQGNDRLY
                     ADRPGSNGPAYLDGGEGDDWMYGTSKADTIIANSGIKKIQGEGGDDLLISTARGAGNF
                     SGGEGRDTISFAAHTPTDRTGPNGGVTVDLGSGNASGASDQNLSGIENVIGSAFDDQI
                     EGAPGVDNDIDGGLGDDFLSGQGGDNLDGGLGENDCSGGSHTNCNENSPGNQRGQTQV
                     DIMEGGIPIVLGSNQADDISVGFNRGAGRFEVAVAGGALPSGLCEKANPAGSQINCPA
                     DPNDLNGLLVYGDNGADQIKLENSIPATLTTTVNGGTGNNTIDGGPSKDFISTAVGSA
                     GSTINGNDGLDVLYAQDRVTVNGGDDTDIFRVVDPCLGAELRGNAGTDSVVFAGAPNG
                     VKASLAGGYAEWRNGGCGSDTDIARDIEKLEGTDYNDWLIVGKRNSSQDGRSVLFGRG
                     GIDILDAKNGSVDQITTGAGGRNNTVIADKNDRITWGYGLGDY"
     gene            4562..>6322
                     /locus_tag="PLE93_00535"
     CDS             4562..>6322
                     /locus_tag="PLE93_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNH85561.1"
                     /translation="MFEGKSAGRLVRILVLALALLVAAAGQLFLTARAEARPSSCFGK
                     TVNRVVKSSNTTVHLAYRDVAWVEGTGVTVIGKPYSRICAGGGRQVIHAGKGTSFTDA
                     GPGNDRIVLHPKSNDNQVHGGAGDDEIDGSSGNDFIYGSPRRLPSGVSDSDDLVGKGG
                     NDHIYDYGGTGNQLRGLTGSDRLYSLGRAVSQVHGGNGTDFIFSNGGKSRSGVIEKVF
                     GEQGNDRIRADRTPNNGPAYLDGGEGDDWMTGSKRDDIFIYNAGIKKLRGLGGDDLFV
                     TTGRGLGRINGGGGTDTISFAALTSSNAYVPGMGPISGARVDLRTGRSIGYTTYRLSA
                     IEDVIGSAFDDWIRGKSGTAHIDGGLGHNICSGGRELQNCNGNSPGGGNGSRAIVDIN
                     ESGFPIVMGSRGADQVKVGFNGASSRFEVQVAGGAAVSGLCRRANSSGSRINCPANRN
                     NLNGLTISTGDGGDSVKLLGSIPATMTTKIDGGNGRNVIQGGRSKDLIETSTSSAGSV
                     LKGDGGLDIIYANDRVRVMGGGDTDISRVVNPCRGATLDGGPGTDSVVFAGPHARRGV
                     KADLGRGYAQWRSGGCAARTD"
BASE COUNT         1189 a         2053 c         2116 g          964 t
ORIGIN      
        1 gctctcgccc tcgcggtgtt ctcgtactcg cagggccagg gtggtccggt tgccggcctc
       61 gtcttcttcg gtgtgctgtt cattggcgcg ttgattcacg tctgccagcc gctcatcgaa
      121 aagggcaagg cgctccggcc ctcaaaggac taaccccatg aagatcgggg tgttgaccgg
      181 cgggggtgat tgccccggcc tcaatgccgt gatccgtgcg gtggtgctca gaggaacccg
      241 tgaatggggt cacgagttca ccggctatct cgacggctgg aagggtccgc ttgaaaacga
      301 gtcgatcgaa ctcggggttg gcgacgtccg cggcatcctc gcccagggcg gaaccatcct
      361 cggttcgtcc cgaaccaacc cggaagcgat cgaaggcggg gtggaccagg tgatcgacaa
      421 tgtccgggcg gccggcctcg acgggctgat cgcgatcggc ggcgacgaca ccctcggcgt
      481 cgcccggatc ctcacggaga gcggactcgg agtggtcggc gtgccgaaga cgatcgacaa
      541 cgatctgaac gccaccgatt acaccttcgg tttcgacacc gccgtcaaca tcgtgatgga
      601 gtccctggac cggctgcaca cgaccgccac cagtcatcac cgctgcctgg tggtcgaggt
      661 gatgggtcgt cacgccggct ggatcgcgct tcacggcggg atggccggtg gcgccgacca
      721 gattctcgtg ccggaagagg agttcgacct cgagcaggtc tgccgccagg tcaaccgcag
      781 cttcgaggcc ggggttgccc cgatcgttgt cgcttcagag ggcgcgatcc cggcggaagg
      841 cagcgagctc accacggccg aggggaccga cgccttcggt cacgtgcggc tcggcggcat
      901 cggcggctgg ctggcggaac ggatcgagga gaacacgggg cacgagacga ggtcggtcgt
      961 gctcggccat ctccagcggg gtggaacccc gaccgccttc gaccgggtgc tggccacgcg
     1021 gcttggaagc cgggcgatcc aggcggccaa cgaaggcgaa tggggcacca tgaccacgct
     1081 tcggggtacc tcgatcgaac tcgccccgct ggccgacgcc gtggacgaat cgaaagtcgt
     1141 cccgccggag cggcttcgcg aagcacaggc tctcttcgac tgaatcccga atcatcctgc
     1201 cctcgggaaa gggaaggaag ttcacaacgg ggatcgctca gtcgagcagg ttggcgaggg
     1261 cgccggccag ttccggctgc ttgaactcgt aaccgccgtc ggtcgccttc ttggggaaga
     1321 ccctctgacc ctcacgggcg gcatggccga ctccgccgcc cttgacgatg tcaatcgcga
     1381 agccgggcac gggcaggaac gagggccggc ccagctgctt gccgagtgct ttcgagaatt
     1441 ccttgttggt taccgggtta ggtgccgtcg cgttgaccgg tccgctgacc gactcgtttt
     1501 caagggccca gagcaggatc ccgatctcgt cgtgccggtg gacccaggac atgtactgct
     1561 tgccgccggc gatcggcccg ccaaccccgg ccttgaacgg cagcatcagt tccttcagca
     1621 acccgccccg ggggtcgagc acgtggccgg agcggatcgt cgccacccgc aggccgtgat
     1681 cctcggccga gcgggccgct tcttcccagc gaaccacgat gtccgccagg aaatcgtccc
     1741 cgggctcggc atcttcatag agaagctcgt cgccacggtc gccgtagtag ccgatcgcag
     1801 aagcggagat cagggtcttc ggccgctggt cgagggcggc gatcttggcc acgaggttct
     1861 tggtcgcctt gatgcggctg cgggcgatcc ggtcctttac ctcgtcgttc cagcgctggt
     1921 ttatcggctc cccggcgagg ttgatcacgg cgtcgacacc ttcaaaagca tctctgggag
     1981 gcctttcgcg ttcgccttcc cagccgtgcc actcaacccg ggggaggctg cgggaagccc
     2041 tctccgggtc acggctcagg ccaaccacgg aatcgccgcg gtcgatcagg gcgttgcaaa
     2101 gggttgagcc gatcaggccg gtcccaccag tgacgagaat gcgctcgggg cgcttggaat
     2161 tcgggtcaga catcctgaac tagtctagag ttccgggtaa cggggcatcg aatgcctcgt
     2221 cgcggtgtta gcaaggggaa cagggatgag attggtcacg tccaaccgga gtcgcacctg
     2281 gaccgttgca gcggccacgg ctgcccttgc ccttttcggg atcctttcgg gtcccgggcc
     2341 cgccggggcg gccccggcca gatgcttcgg ccagaaggtc gaccgcgtga tcaccggttc
     2401 caacaagacc gtcaacgtcg gggcccggga agtcgtgttc gtcgaaggca accggatcac
     2461 cgtcaaggcc acggcgttca gccggatctg cgccggggac ggatcccaga cgatcgaggc
     2521 ggggcacggt ggaccgggca acgacaggat ttccgctgcc aagggcgatt cgacggtttt
     2581 cggcggaccg ggcgacgatc agatcgagac cgaaaagggc aacaacatcg aggtccaggg
     2641 tggccccggc gatgacgaga tccgtcccgg caagggaaac aacgtcaagg tcgacggtga
     2701 cgaaggcaac gaccggatct tcctattcac ccagaccgcc aaaggcgtcg tcgacggtgg
     2761 tctcggcaat gaccggatcg aaggctccca gagccacgac aagctgatcg gcggccccga
     2821 aaagaacccg cggaacctgc ctgacaccga cctgatcatt ggcgagggtg gaaacgactc
     2881 gattttcgat tacggcggca gcggcaaccg gctgatcggc ctcgagggcg ccgacgagct
     2941 ttccagcctc ggcgatgcgg tttccgaact tcatggtggc aacgggtccg acaagctgtt
     3001 ctccaacggt ggccggaccg ccgccggggt cacggaacag ctgttcggcg agcagggcaa
     3061 cgaccggctt tacgcggacc gtcccggcag caacggaccg gcctacctcg acggcggcga
     3121 aggtgacgac tggatgtacg ggacctcgaa ggccgacacg atcatcgcca actcggggat
     3181 caagaagatc cagggagaag gtggcgacga cctgctgatc tcgaccgcgc gcggggccgg
     3241 gaacttcagc ggcggcgaag gccgggacac gatctcattt gccgcccaca ccccgaccga
     3301 ccggaccggg ccgaacggcg gcgtgacggt cgatctcggc tcgggcaacg cttccggcgc
     3361 ctcggaccag aacctgagcg gcattgaaaa cgtgatcggc tccgctttcg atgaccagat
     3421 cgagggcgcg ccgggcgtcg acaacgacat cgacggcggg ctcggagacg acttcctctc
     3481 cggccagggc ggcgacaacc tcgacggcgg gctcggagaa aacgattgct ccggcggcag
     3541 ccacaccaac tgcaacgaga actcccccgg caaccagagg gggcagacac aggtcgacat
     3601 catggaaggc ggaatcccga tcgtcctcgg cagcaaccag gccgatgaca tttcggtcgg
     3661 cttcaatcgc ggcgccggac ggttcgaagt cgcggtcgcc ggcggggccc ttccatcggg
     3721 gctctgcgaa aaggcgaacc cggccggcag ccagatcaat tgcccggccg acccgaacga
     3781 cctcaacggg ctgctcgtct acggtgacaa cggcgccgac cagatcaaac tcgagaactc
     3841 gattccggcg accctgacca cgacggtcaa cggaggcacc ggaaacaaca cgatcgacgg
     3901 cggtcccagc aaggacttca tttcgaccgc ggtcggcagc gccggcagca cgatcaacgg
     3961 caacgacggc ctcgacgtcc tttacgcaca ggaccgggtc accgtcaacg gcggcgatga
     4021 cacagacatc ttccgggtcg tcgatccctg cctcggagcg gagctgcgtg gcaatgccgg
     4081 caccgacagc gtggtgttcg cgggtgcccc gaacggcgtc aaggcaagcc tggcaggcgg
     4141 ttacgcggag tggcgaaacg gcggctgtgg cagtgacacg gacatcgcca gggacatcga
     4201 gaagctcgaa ggaaccgact acaacgactg gctgatcgtc ggcaagcgga actcctccca
     4261 ggacgggcgg tccgtgctct tcggtcgcgg gggcatcgac attctggacg cgaagaacgg
     4321 cagcgtggac cagatcacca ccggcgccgg cggccgcaac aacacggtga tcgccgacaa
     4381 gaacgaccgg atcacctggg gctacggact gggcgattac tgaggccccg ccccggatcg
     4441 ggccggttcg caggcgggat tgtttacttc cgttcgcctg cgggaacaac agtgacttcg
     4501 caactgttac aggttgtcac gaggagctcc ctggacatga gtacccacga cgggagatca
     4561 aatgttcgaa ggtaaatctg caggtcgtct ggtcaggatc ctggttctgg ccctggccct
     4621 tctggtcgcg gcggccggtc aacttttcct gaccgccagg gccgaagccc ggccctccag
     4681 ctgcttcgga aagacggtca accgcgtcgt caagtccagc aacacgacgg ttcacctggc
     4741 ctaccgggat gtcgcctggg tcgaggggac cggcgtcacc gtgatcggca agccctacag
     4801 ccggatctgc gctggcggtg gccgtcaggt catccatgcc ggcaagggca cctccttcac
     4861 cgacgccgga ccgggcaacg acaggatcgt cctgcacccg aagagcaacg acaaccaggt
     4921 tcacggcggc gccggtgacg acgagatcga cggctcgagc ggaaacgact tcatctacgg
     4981 gtctccccgt cgcctgccct ccggggtcag cgacagcgac gacctggtcg gcaagggcgg
     5041 caacgatcac atttacgact acgggggaac cggcaaccag cttcgcggcc tgaccggctc
     5101 cgaccgcctg tacagcctcg gcagggcggt ctcccaggtg cacggcggca acggcaccga
     5161 cttcatcttc tccaacggcg gcaagagccg aagcggagtg atcgagaaag tgttcggcga
     5221 gcagggcaac gaccgcatcc gggccgaccg gacaccgaac aacggccccg cctatctcga
     5281 cggaggcgag ggtgacgact ggatgaccgg cagcaaacgc gacgacatct tcatttacaa
     5341 cgccggaatc aagaagctga gggggctcgg cggagacgat ctcttcgtaa ccaccggccg
     5401 cgggctgggc aggataaatg gcggcggcgg aacggacacg atttccttcg ctgcgcttac
     5461 ttccagcaac gcctacgtgc cggggatggg cccgatcagc ggggcccggg ttgacctccg
     5521 gaccgggcgc tcgatcggct acacgaccta ccgcctgtcc gcgatcgaag atgtgatcgg
     5581 gtccgcattc gacgactgga tccgcggcaa gtccggcacc gcccacatcg acggcgggct
     5641 gggccacaac atctgttccg gcgggcgcga gctgcagaac tgcaacggga attcacccgg
     5701 cggcggcaac ggctcgaggg cgatcgttga catcaacgag agcggtttcc cgatcgtgat
     5761 gggcagtcgc ggggccgatc aggtcaaggt cggcttcaac ggcgcatcca gccggttcga
     5821 ggtgcaggtc gccggcggcg cggccgtctc cggtctctgc cgcagggcaa actcttccgg
     5881 cagccggatc aattgcccgg ccaaccgcaa caacctgaac gggctcacga tctcgaccgg
     5941 tgacggggga gactcggtca agctgctcgg ctcgatcccg gccacgatga cgaccaagat
     6001 cgacggcggc aacggtcgca acgtgatcca gggaggtcga agcaaggacc tgatcgaaac
     6061 ctcgaccagc agcgccggca gcgtgctcaa gggcgacggc ggcctcgaca tcatctacgc
     6121 caacgaccgg gtccgggtga tgggcggtgg cgacaccgac atctcccgcg tggtcaaccc
     6181 ctgccgtggc gccacgcttg acggcggccc cggcaccgac agcgtcgtct tcgccggtcc
     6241 tcacgcccgg cgcggcgtaa aggccgacct cggccggggc tacgcacagt ggcgaagcgg
     6301 cggctgtgcg gcccggaccg ac
//
LOCUS       DAOQFI010000014         6290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_4651_length_6290_cov_3.317883, whole genome shotgun sequence.
ACCESSION   DAOQFI010000014 DAOQFI010000000
VERSION     DAOQFI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4651_length_6290_cov_3.317883"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLE93_00540"
     CDS             <1..182
                     /locus_tag="PLE93_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85562.1"
                     /translation="TACLKSRRNLAAARKRLVTAKKSLKKAKSQPAKKAARTRVMRIG
                     KDVGRFQKSVARNCG"
     gene            227..2077
                     /locus_tag="PLE93_00545"
     CDS             227..2077
                     /locus_tag="PLE93_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85563.1"
                     /translation="MPRKFAQGLTAFGLAVAIIFGFTGNAAAAPADRSGDAVVLKGSE
                     IARLLGADPARLVGFSFRNGRWSQIPVQVDERHTVDVRSLYPPENRTYVGDSSPDFNL
                     EVYADPKTRSGADEDATFDANDELTFMGGDTGSRVPAGVGAPAPVVPGSGTAVRGSDP
                     VGGGNAWVYLFRSINRKFDPSAGKDYVDYDFKLTGLTGGQTLLNDYGYFSQFNPEDST
                     VKTDDYELHSYDRWMEDELKIRAGNANGTDILDREVAQAGRGSCGRSEYTFSGRWGQD
                     STSGNDNSTDNEGTYVAVIDGPVRAIRSYMGANSGPYVQREHIYYRDHEQNTIFLRVH
                     QMADLYTWTDFAPSAIGMTYRDAKNTAGVTVDGNPDSIAPTTSADISNGAYSWQQISG
                     PQGTATTVMGSDTDLTDPNFGNYYLDDSTPSGPLEVQCGGDGQSIGASGFGIFGEGDT
                     GITPNTDPRSAGFKTLTTRRTRYFGPPADGVAKAENYTARVSSPLTATSSPSPMKQGM
                     GSARAYILTRGVTAHRGRAIRLRVKVRNSGNFRIRKAKACVFGKGLTGRPCTRVNDLG
                     IGNERTVTLKTRVGRFAKGRKLKLSVSVTGSAGDWGFAGVGGYFLIPLRG"
     gene            2135..3955
                     /locus_tag="PLE93_00550"
     CDS             2135..3955
                     /locus_tag="PLE93_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85564.1"
                     /translation="MAVLGLAAAISTGIGGVASAAPADRGNDPVVLKGSQVAGLDGVA
                     PGRIVGFKWEGKWVQVPVQVDQRHTVSARGLYPDEAPAYVNNDLTFDVEIYADAKTRS
                     GADADPNLDPDDEIAFMGSDTGDAAPAATLSPKGVIGSTATRIDVVDPIDTSVAQVYL
                     FKSDGSLDPSAGKDYVDYDFNLTNLGPGETLIDDYGFSNSNNPEDSTVSTANYTLHST
                     DRWMEDEIKIKAGGASDTDILDREAASAGGLGGCGRSEYTFSGNWNRGSDNDEGTILA
                     IKDGPVRAIRDYMGANSGPYVEDTHVYYSDREDRMIHVRVHPIPLMYVWTDYNDQAVG
                     MTYRDQLNPTGVKIDGVPGEQDGQPFDDSIDKATPADVPSGQYLWQQVTGAQGTATTL
                     VSADSSVEPGGIFNGFAGYYLDDSTPTGSSERQCGGDMKAYGASGFGIDGVYPNTDPN
                     LATTASPAAKLSVNRIRYFTSPNGTAADADGLRDRAQDPLTGSSADANIKARAAKLKV
                     TLQGPAPKAKPGRQLRLKLKIANSGTADSAEGKVCVAGVALPHKTCRNFARIFRQKST
                     TVTLTPTVKKTVKANKLVLKVTATSGKASDGLTLKLPLDR"
     gene            complement(3966..4922)
                     /locus_tag="PLE93_00555"
     CDS             complement(3966..4922)
                     /locus_tag="PLE93_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNH85565.1"
                     /translation="MFETRTHQWHEAGLGEQIVGPPQGRTWPRLILFAVLIAAVLVAF
                     NHRQDIAPGYGTEARILTAILLFGLGWGFASALGRTVTPFIMRRLDPGTAGTLGFVIR
                     LGTILIVGLVALGIAGVKPQTLAVGGAATAIVLGLAAQQTLGNVFAGVVLQSTRPFRV
                     GERVRLTGGPMAGSVEGTVSSLGLFYTTILKGQDRMMIPNNLLLLLAVEPLREPDSID
                     LKARFDSHLSPATVQEMLRQAITVPTLRPPRIELEEVDRDEVVLRISATPVNPSDGAR
                     LAEQVVSVTRGTFEYEKPDPEAIAREKGPDQKPDPDVPHGHS"
     gene            complement(5065..>6290)
                     /locus_tag="PLE93_00560"
     CDS             complement(5065..>6290)
                     /locus_tag="PLE93_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85566.1"
                     /translation="TFDLDSDQMPIRSEGNTMQARFSCNIPRSVVGDGAGGGDDTQFH
                     QTARPSLYGHGLFGSINEVTSRNIRQLGVENNVLVCGSDWVGMADQDIGSAINALLDL
                     SKFPALPDRLQQGFLNFLFLGRLMIHPDGFAADPAFKVDGHSVIDTSDLFYYGNSQGG
                     IAGGALTAVATDFTRSVLYVPGMNYSMLLTRSTDFEDYKSILYPAYQNELERPSIFAL
                     MQSMWDRGEPNGYANNMTSNPLPNTPAHHVMIEMSYGDHQVANVATEVEARTIGAPLR
                     TPTIDADRAATGLIEPFYGHDTLGDLAGPAADGNAYFVWDIGPQRLQEDPEGSGNMKL
                     FGTDPAPLTNTPPVTGESDTPLGETSGIDPHDTVIEESPLIRKQIADFLKTDGKVTNP
                     CGLNPCYAAKWKGMP"
BASE COUNT         1168 a         2058 c         2051 g         1013 t
ORIGIN      
        1 cgaccgcctg cctcaaatcc aggcggaacc tcgccgccgc ccgcaagcgg ctggtcacgg
       61 cgaagaagtc gctgaagaag gcgaagagcc agccagcgaa aaaggccgcg cggaccaggg
      121 tgatgaggat cggcaaggat gtcggcagat ttcagaagtc ggttgcccgc aactgcggct
      181 gacctggtct gttcaaatca ccgtcgacca acgatcaagg agaaacgtgc ccaggaagtt
      241 cgcgcaggga ctgactgcct tcggcctggc cgtggcaatc attttcggat tcaccggcaa
      301 cgccgccgcg gcaccggcgg accgctccgg agatgccgtc gtgctcaagg gcagtgaaat
      361 cgccaggctg ctcggggccg acccggcgcg cctggtcggc ttctccttca ggaacggtcg
      421 ctggagccag atcccggtgc aggttgacga gcgccacacg gttgacgtgc gcagccttta
      481 cccgcccgag aaccgcacct acgtgggaga cagcagcccg gatttcaatc tcgaggtcta
      541 cgccgacccc aagaccaggt cgggcgccga cgaggacgca acctttgacg ccaacgacga
      601 gctgaccttc atgggcggtg acaccggcag ccgggttccg gccggcgttg gtgctcccgc
      661 cccggttgtt ccgggctcgg gaacggcggt cagggggagc gacccggtcg gtggcggcaa
      721 cgcctgggtc tatctcttcc gatcgatcaa ccggaagttc gacccttcgg ccggcaagga
      781 ctacgtcgac tacgacttca agctgaccgg actgaccggg ggacagaccc tgctgaacga
      841 ctacggctac ttcagccagt tcaaccccga ggactcgacc gtcaagaccg acgactacga
      901 gctccactcg tacgaccgct ggatggaaga cgaactgaag atccgggccg gcaacgcgaa
      961 cggaaccgac atcctcgacc gggaagtcgc gcaggccgga cggggcagct gcggccgctc
     1021 cgaatacacg ttctccggcc gctggggcca ggattcgacc agcgggaacg acaactcgac
     1081 cgataacgag ggcacctatg tcgccgtgat cgacgggccg gtacgggcga tccgctccta
     1141 catgggcgcc aattccgggc cttacgtcca gcgcgagcac atctactacc gggaccacga
     1201 gcagaacacg atcttcctcc gggttcacca gatggccgat ctctacacct ggaccgattt
     1261 cgcgccttcg gcgatcggca tgacctaccg cgacgcgaag aacacggccg gcgtcacggt
     1321 cgacggcaat ccggattcga tcgccccgac caccagcgcc gacatctcga acggggccta
     1381 ctcgtggcag cagatctccg gcccccaggg aacggcaacc acggtgatgg gatcggatac
     1441 cgatctcacg gacccgaact tcggcaacta ctacctcgac gattcgactc cgtccggtcc
     1501 gctcgaggtc cagtgcgggg gtgacggcca gtcgatcgga gccagcggct tcgggatctt
     1561 cggcgagggc gacacgggca taaccccgaa cacggacccc cgcagcgccg gtttcaagac
     1621 tctgacgacc aggcgcaccc gttatttcgg cccgccggct gacggggtcg ccaaagcgga
     1681 gaattacacg gccagggtga gcagcccgct gaccgcgacc agcagcccga gcccgatgaa
     1741 gcagggcatg ggttcggccc gcgcctacat cctcacccgg ggcgtaaccg cgcaccgtgg
     1801 ccgggcgatc aggctccggg tcaaggtacg aaacagcggc aacttccgga tccggaaagc
     1861 gaaggcctgt gttttcggca agggactgac cggcaggcca tgcacccggg tcaacgatct
     1921 cggtatcggc aacgagcgga ccgtgacttt gaagaccagg gtcggccggt tcgcgaaggg
     1981 caggaaactg aagctgagcg tctcggtcac cggcagcgcg ggtgactggg gatttgccgg
     2041 cgtcggcggt tacttcctga ttccactccg cggctgagcg gctatccttc ccgacatggg
     2101 atgggacctc gcgcgtaaga cgttcgcgcg gtcgatggct gtgcttggcc tcgccgcggc
     2161 aatttcaacc gggatcggtg gtgtcgcgtc ggcggctccg gccgaccggg gcaatgatcc
     2221 ggtcgttctg aagggcagcc aggtcgcggg gctcgacggt gtcgcgccgg gccggatcgt
     2281 cggtttcaaa tgggaaggaa agtgggtcca ggttccggtt caggtggacc agcgccacac
     2341 ggtcagtgcc cgcggcctct atcccgacga ggcgcccgcc tacgtcaaca acgacctgac
     2401 cttcgacgtc gagatttacg ccgacgccaa gacccgctcc ggcgccgacg cagaccccaa
     2461 tctcgacccg gatgacgaga tcgccttcat gggcagtgac accggagatg ccgctccggc
     2521 cgccacgctt tcgccgaaag gcgtcatcgg ttccaccgcg acgcggatcg atgtggtcga
     2581 tccgatcgac accagcgtcg cccaggtcta cctcttcaag tccgacggtt cactcgaccc
     2641 ctcggccggg aaggactatg tcgattacga cttcaacctg accaacctcg ggccgggcga
     2701 gaccctgatc gatgactacg ggttctcgaa ctcgaacaat cccgaagatt cgacggtcag
     2761 cacagccaac tacaccctcc actccaccga ccgctggatg gaagacgaaa tcaaaatcaa
     2821 ggcgggtggc gcgagcgata ccgacattct cgaccgcgag gcggcctcgg ccggtggcct
     2881 cggtggctgc ggccggagtg aatacacctt ctccggaaac tggaatcgcg gcagcgacaa
     2941 cgacgaaggc acgattctcg cgatcaagga cggcccggtc cgcgcgatcc gggactacat
     3001 gggcgccaac tccggcccgt atgtggagga cacccatgtc tactactccg atcgcgaaga
     3061 ccggatgatc cacgtgcggg tccacccgat cccgctcatg tatgtctgga ccgactacaa
     3121 cgaccaggcg gtaggcatga cctatcgtga ccagctgaac ccgactggcg tcaagatcga
     3181 cggcgttccc ggcgagcagg atggtcagcc cttcgacgat tcgatcgaca aggcgacgcc
     3241 ggccgacgtg cccagcggcc agtacctctg gcagcaggtg accggcgcgc aaggcaccgc
     3301 gaccactctg gtctcggcgg acagctcggt cgaacccggc ggcatcttca acggattcgc
     3361 cggttactac ctcgacgatt cgactcccac cggctcctcc gagaggcagt gcggcggtga
     3421 catgaaggcg tacggggcca gcggctttgg catcgacggc gtctacccga acaccgatcc
     3481 gaaccttgcc accaccgctt caccggcggc gaagctctcg gtcaaccgga tccgctactt
     3541 caccagcccg aacggcaccg ccgccgacgc agacggcctc cgggaccgcg cccaggaccc
     3601 gctcaccggt tcgtcggcag acgccaacat caaggcccgg gcagccaagc tgaaggtcac
     3661 gcttcagggt cccgctccga aggcgaagcc gggcaggcaa ctcaggctga aactgaagat
     3721 cgcaaacagc ggtaccgcgg actcggctga aggcaaggtc tgtgtggccg gtgtggccct
     3781 cccgcacaag acctgcagga acttcgcgcg gatcttccgc cagaagtcga ccacggtgac
     3841 cctcacaccg accgtgaaga agaccgtgaa ggcgaacaag ctggtcctca aggtcactgc
     3901 cacttcaggc aaggcctcgg atggcctgac gctgaagctc cccctcgacc gctaggaagt
     3961 cccgctcagg aatgaccgtg gggaacatcg gggtcgggct tctggtccgg ccccttttcc
     4021 ctcgcgatcg cttccggatc cggcttctcg tattcgaagg tgccgcgggt taccgagacg
     4081 acctgctcgg ccagcctggc gccgtctgac gggttgaccg gggtggcgct gattcgaagc
     4141 accacttcgt ccctgtcgac ctcctccagc tcgatgcggg gaggccgcag ggtcggcacg
     4201 gtgatcgcct ggcgcagcat ctcctgaacg gttgccgggc tcaggtggct gtcgaagcgg
     4261 gccttcagat caatcgaatc cggttcgcgc agcggctcga ccgccagcag caggagcagg
     4321 ttgttgggaa tcatcatccg gtcctggccc ttgaggatcg tcgtgtagaa gaggccgagc
     4381 gaactgaccg ttccctcgac cgagccggcc atcggcccgc cggtcaggcg cacccgctcg
     4441 ccgacccgga acggccgggt gctctggagc acgaccccgg caaagacgtt gccaagggtc
     4501 tgctgggcgg cgagaccgag gacgatcgcg gtggccgcac cgccgaccgc cagggtttgc
     4561 ggtttgaccc cggcaatccc gagcgcgacc agaccgacga tcaggatcgt gcccagccgg
     4621 atgacgaagc cgagtgtgcc ggccgttccc gggtcgagcc ggcgcatgat gaagggagtg
     4681 accgtgcggc cgagggcgga ggcgaaaccc cagcccaggc caaagagcag gatcgcagtg
     4741 aggatcctcg cctcggtgcc gtagcccggg gcgatgtcct gccggtgatt gaaggcgacc
     4801 aggaccgccg cgatcagcac ggcgaagagg atcagcctcg gccaggtccg tccctgtggc
     4861 gggccgacga tctgttcgcc aaggccggct tcatgccact ggtgggtccg ggtctcgaac
     4921 atccacctcg gcatctgggc ccgggtgcgt tcggtccttt tcttctcacg tcgtgaagcc
     4981 acgacggttc accatacaaa gagcccgggt tgaagctgcc cgcagatcag gcgaacggct
     5041 ccaacccggg cgggaacttc cggcctacgg catgcccttc cacttggcgg cgtagcaggg
     5101 gttgagacca caggggtttg tgaccttgcc gtccgtcttg aggaagtcgg cgatctgctt
     5161 gcggatcagc ggtgactctt cgatcaccgt gtcatgcggg tcgatgccgc tggtctcgcc
     5221 gagcggggtg tcggactcgc cggtgaccgg cggggtattg gtcagcggtg ccggatcggt
     5281 tccgaacagc ttcatgttgc cgctgccttc cggatcttcc tgcagccgct gggggccgat
     5341 gtcccagacg aaataggcgt tgccgtcggc agccgggccg gccaggtcac ccagggtgtc
     5401 gtgaccgtag aacggctcga tcaggcccgt ggcggcccgg tccgcgtcaa tcgtcggcgt
     5461 cctcagcgga gcgccgatcg tccgggcctc gacctcggtt gcgacgttcg ccacctgatg
     5521 gtcgccgtag gacatctcga tcatcacgtg gtgggccggg gtgttcggca acggattgct
     5581 ggtcatgttg ttggcgtagc catttggctc gccgcggtcc cacatcgact gcatcagggc
     5641 gaagatcgag ggtcgctcga gctcgttctg gtaggccggg tagaggatcg acttgtagtc
     5701 ctcgaagtcg gtgctgcgag tcagcagcat cgagtagttc atacccggca cgtagagcac
     5761 cgacctggtg aaatcggtcg ccaccgcggt cagggcaccg ccggcgattc cgccctggct
     5821 gttgccgtag tagaagaggt cactggtgtc gatcaccgag tgtccgtcga ccttgaaggc
     5881 cgggtcggcc gcgaagccgt ccgggtggat catcagccgg cccaggaaca ggaagttgag
     5941 gaaaccctgc tgcaggcgat ccggcagggc cgggaacttg ctcaggtcga gcagcgcgtt
     6001 gatcgcggat ccaatgtcct ggtcggccat cccgacccag tcggagccgc agaccaggac
     6061 gttgttctcg acgccgagct ggcggatgtt gcggctggtc acctcattga tcgagccgaa
     6121 cagaccgtga ccgtaaagcg aggggcgggc cgtctggtgg aactgggtgt catcgccacc
     6181 gccggcaccg tcgccgacca ccgagcgcgg gatgttgcag gaaaaccgcg cctgcatcgt
     6241 gttcccttcg ctgcggatcg gcatctggtc ggaatcgaga tcaaacgtgg
//
LOCUS       DAOQFI010000015         6244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_4708_length_6244_cov_3.566812, whole genome shotgun sequence.
ACCESSION   DAOQFI010000015 DAOQFI010000000
VERSION     DAOQFI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4708_length_6244_cov_3.566812"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1453)
                     /gene="gatB"
                     /locus_tag="PLE93_00565"
     CDS             complement(17..1453)
                     /gene="gatB"
                     /locus_tag="PLE93_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNH85567.1"
                     /translation="MSTEYEAVIGLEIHVQLSTRTKMFCGCELSFGDDPNVHTCPVCL
                     ALPGVLPVPNAQAIEYALQIGAALGCELSPVSTFDRKNYFYPDNPKAYQITQAARPFA
                     VNGRLGEVRIHHAHLEEDAAKTIHTGGGGRIKGSDASEVDFNRGGTPLVEIVTEPDLR
                     SGAQVREWGQLLRATIKQLGVSDVNMEEGSLRCDANVSIRPVGSTEFGTKTEMKNMNS
                     FRFIERGIEAEVARQKEVVASGGQVVQETLHFDPATGELHSLRSKEEAHDYRYLPEPD
                     LVPVVPTPAMLEEAASSLPELPAARAARYEGIGVSEQAALLLSFELEWAGYFEGVLAA
                     DQSIEPRVAANWVTGEFAAELRKQDDQALADSKVGPESLATLIGLVQAKKISHGAGKT
                     VLEEMVASGTDPVTVVAEKGLEQIADSGELEKIVIQAIEDNPKPAEQVREGNEKAIGA
                     LVGAVMKATQGRADGGEVNRLLRQHLGL"
     gene            complement(1450..2907)
                     /gene="gatA"
                     /locus_tag="PLE93_00570"
     CDS             complement(1450..2907)
                     /gene="gatA"
                     /locus_tag="PLE93_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNH85568.1"
                     /translation="MADIELLAASGAETAAKIKSGELSADEVLAFWLDRAGRDRLNGY
                     LWQADRDSARQGLGEGGPLDGVPLAIKDIFCTEGVPTTAASRILGGYVPPYTATSVRK
                     AMAAGMPMLGKTNMDEFAMGSSNENSAFGACLNPWDTERVPGGSSGGSAAVVAGGLAP
                     WSLGTDTGGSIRQPAGFCGIVGLKPTYGAISRFGMIAFASSLDQCGPLTRNVTDAALL
                     LNVLQGRDEMDSTSVGIEGGIKAPDAERLDGMTFGVPRALMGEGIEAGVLARFEDTLI
                     RIAELGGEIVDIELPHAGHAISAYYVLAPAEASTNLSRYDGVRYGLRASNEDLLSMYE
                     VTREDGFGPEVKRRIMLGTYALSSGYYDAYYGRAQRVRTRIVEDFEKAFGEVDFVVTP
                     TSPTVAFKLGEKTSDPWTMYLSDVFTVPISLAGVPAISIPAGLAEPDGGGSELPVGFQ
                     IAAPAFAEQKLLEASYALEQAIGFDGSAALEGGVS"
     gene            complement(2910..3200)
                     /gene="gatC"
                     /locus_tag="PLE93_00575"
     CDS             complement(2910..3200)
                     /gene="gatC"
                     /locus_tag="PLE93_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNH85569.1"
                     /translation="MIEREQVLHVARLSRLRLGDEEIDTLAGELSSILDHVDKLAEVD
                     IEGVEPTSHVVPLENVLRPDEPRPSLDRELALSQAPDPHDGAFRVPSPQAEF"
     gene            complement(3247..3597)
                     /locus_tag="PLE93_00580"
     CDS             complement(3247..3597)
                     /locus_tag="PLE93_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85570.1"
                     /translation="MKLSRVHPFEWLVGLIGLILVAGIVSTVSSPGTLHVVVLLVAAA
                     GAMLPVTVSMSARTNVPIIFETMLWIVSILVSLILLFKAIFPSDVVFESGWLTLAGML
                     AMSFALWRSVAREQ"
     gene            complement(3594..4445)
                     /locus_tag="PLE93_00585"
     CDS             complement(3594..4445)
                     /locus_tag="PLE93_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNH85571.1"
                     /translation="MSAKIIDGKAVSAEVRERVAAEVSEFIEGGGHQPVLATVLVGDD
                     PASHVYVANKHKACEAAGIGSVHHELPAETSQADLVTLVEALGDDDAIDGILVQLPPP
                     EHIDPDAVIAAINPAKDVDGLTATNAGLLAGGRPGLVPCTPAGVIELLDHAGTELEGA
                     EAVIVGRSNLVGRPLISLLLGRNATVTAAHSRTRDLAAVCSRADVLVAAVGVPELLGA
                     DHVKPGATVIDVGINRTDDGLKGDVDFEAAVEVAGAITPVPGGVGPMTIAMLMANTLA
                     AARARAI"
     gene            complement(4473..>6244)
                     /locus_tag="PLE93_00590"
     CDS             complement(4473..>6244)
                     /locus_tag="PLE93_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNH85572.1"
                     /translation="YFEDTAAGVYALYEQRMVESNAMDFDDLLVRMVNVLELFPEVRE
                     RWRRAFRYVLVDEYQDTNHAQYRLLQLLCGEHRNLTVVGDEDQSIYGFRHADIRNILD
                     FGEDFPDAKVVKLEQNYRSTQTILSAANAVVENNRDRRPKKLWTDLGQGDEIEVVRLS
                     DEHEEARWVAGEIERLVEDEGISPLDVAVFYRMNAMSRVLEDTLVRFGTAYRVIGGTR
                     FYDRAEIKDAIAYLQYLANPRDSVAFTRIVNSPKRGIGNTTQGRLLAFANTTGDDIWD
                     VASQVESVPGMGTAAIRAVSEFQRTMRGLRDDAEGRPLADLLERVLDETGYFEALESE
                     RTVEAEGRVENLRELIGVAGEFDTNRALEGESEVEPLDEFLQQISLYSEQDKLTEREE
                     LLTLMTLHNAKGLEYDTVFIIGCEDGIFPHQRALDEGEEEEERRLCYVGITRARKRLY
                     LTSARTRRIFGGRSEATMPSRFLDELPAKLVHQQGSAVGQGGFSTGYSGSGYGGSGRA
                     RSYQAPGRPRPDGRSGGPAATRIEPGTAVHFEVGDDVTHGTFGDGVVIGAEPGGVIIV
                     RFANDGSERKLMAEYAPIRKKSN"
BASE COUNT         1020 a         2073 c         2040 g         1111 t
ORIGIN      
        1 cgctgagaag cgtgaactaa aggcccaggt gctgacggag gaggcggttg acctcgcctc
       61 cgtcggcgcg gccctgggtg gccttcatca cggctccgac cagggcgccg atcgccttct
      121 cgttgccttc ccggacctgt tcggccggtt tcggattgtc ctcgatcgcc tgaatgacga
      181 tcttctccag ctcgcctgaa tcggcgatct gttccaggcc cttttcagcc accacggtga
      241 ccggatcggt tccggaggcg accatctcct caagcaccgt cttgccggcc ccgtgggaga
      301 tcttcttcgc ctgaaccagg ccgatcagcg tagccaggga ctccggcccg actttcgagt
      361 cggccagggc ctggtcatcc tgtttgcgca gctcggcggc aaactcgccg gtcacccagt
      421 tcgcggcaac ccgcggttcg atcgattgat cggccgccag cacgccctcg aagtacccgg
      481 cccattcgag ctcgaacgag agcagcaggg cagcctgttc cgatacgccg atcccttcgt
      541 agcgggcggc ccgggcggcc ggcagttcgg gaagcgagct cgcagcttcc tccagcatcg
      601 ccggggtcgg cacgaccggg accagatcgg gttccggcag gtagcggtag tcgtgtgctt
      661 cttccttgga acggagcgag tgaagctccc cggtcgccgg atcgaagtga agtgtttcct
      721 ggaccacctg gccgccgctc gcgacgactt ccttctggcg ggcgacctcg gcctcgatgc
      781 cgcgttcgat gaagcggaag gagttcatgt tcttcatctc cgtcttggta ccgaactcgg
      841 tgctgccgac cgggcggatc gagacgttgg cgtcgcagcg cagcgagcct tcctccatgt
      901 tcacgtcgga gacgccgagt tgcttgatcg tcgccctcag gagctggccc cattcccgga
      961 cctgggcgcc ggaacgaagg tccggttcgg tgacgatctc taccagcggg gttccgcccc
     1021 ggttgaaatc gacctccgag gcgtcggaac ccttgatccg gccgccgcct ccggtgtgga
     1081 tcgtcttcgc cgcatcctcc tcgaggtggg cgtgatggat ccgtacttcg ccgagccggc
     1141 cgttgaccgc aaagggcctg gctgcctggg tgatctggta cgccttgggg ttgtccggat
     1201 agaagtagtt cttccggtca aaggtggaga cgggggaaag ttcgcagccg agagcggcgc
     1261 cgatctgaag cgcgtactcg atcgcctggg cgttgggcac cggcagcacg ccgggcaggg
     1321 ccaggcaaac ggggcaggtg tggacgttcg gatcgtcacc gaaggagagt tcgcagccgc
     1381 agaacatctt ggttctggtc gaaagctgga cgtgaatctc cagcccgatc accgcttcgt
     1441 attcggtgct cacgagacac ccccttcgag ggcggcggaa ccatcgaagc caatcgcctg
     1501 ttccagcgcg taggaggcct cgagcagctt ctgctcggcg aaggcggggg cggcgatctg
     1561 gaagccgacc ggcagttcgg agcctccccc atccggttcg gccagtccgg ccgggatcga
     1621 gatggccggc accccggcca gcgagatcgg caccgtgaac acgtcactca ggtacatcgt
     1681 ccaggggtcg gaggtcttct cgccaagttt gaaggcaacg gtcggcgagg tcggcgtgac
     1741 cacgaagtcg acctcaccga aggccttctc gaagtcttcg acgatgcggg tgcgaacccg
     1801 ctgggcccgg ccgtaatagg cgtcgtagta gcccgaggaa agggcgtagg tcccgagcat
     1861 gatgcgccgc ttgacctcgg gcccgaagcc gtcttcgcgg gtcacctcgt acatcgagag
     1921 caggtcttcg ttgctggccc tgaggccgta acggaccccg tcatagcgtg agaggttggt
     1981 cgaggcttcg gccggggcga gcacgtaata ggcggagatc gcgtgcccgg cgtgaggcag
     2041 ttcgatgtcg acgatctcgc cgccgagttc ggcgatccgg atcagcgtgt cctcgaaccg
     2101 ggccaggaca ccggcttcga ttccctcgcc catcagggcc cggggcacgc cgaaggtcat
     2161 gccgtcaagc cgttcggcgt ccggcgcctt gatcccgcct tcgatgccga ccgaggtcga
     2221 atccatttcg tcacgcccct gaagcacgtt caggagcagg gccgcgtcgg tgacgttcct
     2281 ggtcagcggc ccgcattgat cgagagaaga ggcgaaggcg atcatcccga agcgggaaat
     2341 cgcgccgtag gtgggcttaa ggccgacgat cccgcagaat ccggccggct ggcgaatcga
     2401 acctccggtg tcggtgccga gcgaccaggg ggcgagtccg ccggcgacga ccgccgccga
     2461 cccgcccgaa gatcctcccg gcaccctttc ggtgtcccag gggttgaggc aggcaccgaa
     2521 ggccgaattc tcgttcgagg agcccatcgc gaactcgtcc atgttggtct tgccgagcat
     2581 cggcatcccc gccgccatgg ccttcctcac cgaggtggcc gtgtatggcg gcacgtagcc
     2641 gccgagaatc ctcgaggcgg ccgtggtcgg cacgccttcg gtgcaaaaga tgtccttgat
     2701 tgcgagcggc accccgtcga gaggaccccc ctcgcccagt ccctggcggg cagaatcacg
     2761 atcggcctgc cagaggtatc cgttcagccg gtcgcgaccg gcccggtcga gccagaaggc
     2821 gagtacttcg tcggccgaaa gctcgccgct tttgatcttt gccgccgttt cagcaccgct
     2881 ggcggccagc agctcgatat cggccatgct cagaactccg cctgcggtga aggaacccgg
     2941 aaagcgccgt cgtgggggtc gggagcctgc gaaagggcga gttcccggtc aagactgggg
     3001 cgcggttcat ccggccgcag cacgttctcc agcggcacca catgcgaggt tggttcgacc
     3061 ccctcgatgt ccacctccgc cagtttgtcg acgtggtcga gaatcgagga aagctcaccg
     3121 gcgagggtgt cgatctcttc gtcgccaagt cgcagccggg agagccgggc gacatgcaga
     3181 acctgttcgc gctcaatcac gatggtgagt ctaagggcgg cgaagcgagt acgtcagccc
     3241 gccgggctac tgctcccggg cgacgctgcg ccacagtgcg aagctcatgg cgagcatccc
     3301 cgccagggtc agccagccgg actcgaagac aacgtccgac gggaatatgg ccttgaacag
     3361 gagaatcaag ctgaccagaa tggagacgat ccagagcatc gtctcgaaga tgatcggcac
     3421 gttcgtccgc gcactcatcg acaccgtcac cggcaacatg gcccctgccg cggcgaccag
     3481 aaggaccacc acgtgcagag tgccgggcga ggagacagtc gagacaattc cggccaccag
     3541 gatcaggccg atcaatccga cgagccactc gaacggatgg acgcgggaga gcttcaaatg
     3601 gcccttgccc ttgcggcggc gagcgtgttt gccatcagca tcgcgatcgt catcggcccg
     3661 accccgcccg gcaccggggt gatcgcgccg gccacctcga ccgcggcctc gaagtcgaca
     3721 tcacccttga ggccgtcgtc ggtgcggttg atgccgacgt cgatcacggt cgcaccgggt
     3781 ttgacgtggt cggcgcccaa aagttcgggt acgccgaccg cagcgaccag cacgtcggcc
     3841 cgggagcaga cggcggcgag gtcacgggtc cgggagtgag cggcggtgac ggttgcgttg
     3901 cggcccagca gcaggctgat cagcggcctg ccgaccaggt tcgaacggcc gacgatcacc
     3961 gcctcggccc cttcgagttc ggttccggcg tggtcgagca gctcgatcac cccggccggt
     4021 gtgcagggca ccagaccggg ccggccgccg gccagcagtc cggcgttggt cgcggtcagg
     4081 ccgtcgacat ccttggccgg gttgatcgcg gcgatcaccg cgtcgggatc gatgtgttcc
     4141 gggggcggaa gctggacgag aattccgtcg atcgcgtcgt catcgccgag cgcctcgacc
     4201 agcgtgacca ggtcggcctg gctggtttcg gcaggcagtt cgtggtgaac cgacccgatc
     4261 ccggctgctt cgcatgcctt gtgcttgttc gcgacataga catgggacgc ggggtcgtcc
     4321 ccgaccagca ccgtggccag caccggctga tggccgccgc cttcgatgaa ctcgctcact
     4381 tcggccgcaa ccctctcgcg aacctcggct gaaacagctt tgccgtcaat gatctttgcg
     4441 ctcaaccgcc tgaccttccg tccgcttgct tttcagttcg acttcttccg gatcggcgcg
     4501 tactcggcca tcagcttccg ctcgctgccg tcgttcgcga accggacgat gatcactccg
     4561 ccgggttcgg cgccgatcac gaccccgtca ccgaaggtgc cgtgggtcac atcatcgcca
     4621 acctcgaaat gaaccgccgt gccgggctcg atcctggtcg ccgccgggcc gccagacctt
     4681 ccgtcgggcc gcgggcgtcc gggggcctgg tagctgcggg cccggccgct gccaccgtag
     4741 ccgctacccg aatagccggt cgagaagccg ccctgcccga ccgccgaacc ctgctggtga
     4801 accagcttcg ccggcagttc atcgagaaaa cgggatggca tggtcgcctc ggagcggccc
     4861 ccgaagatcc gcctggtccg ggcgctcgtc aggtaaagcc gtttcctggc ccgggtgatg
     4921 ccgacgtaac agagccgccg ttcctcttcc tcttcacctt cgtcgagggc ccgctggtgg
     4981 gggaagatcc cgtcttcgca gccgatgatg aagaccgtgt cgtactcgag gcccttggcg
     5041 ttgtgaaggg tcatcagggt aagcagctct tcccgttcgg tcagcttgtc ctgctccgag
     5101 tagagcgaga tctgctggag gaactcgtcc agcggttcga cctcgctttc accctcgagc
     5161 gcgcggttcg tgtcgaactc gccggccacg ccgatcagtt cccggaggtt ctccaccctg
     5221 ccttcggctt ccacggtccg ttccgactcg agcgcctcga aatacccggt ctcgtccagg
     5281 acccgctcca gcaggtcggc cagcggccgg ccctcggcgt catctctcag gccccgcatg
     5341 gttcgctgaa actcggacac cgcccggatt gccgcggtcc ccatgccggg aacactctcg
     5401 acctggctgg ccacgtccca gatgtcgtca ccggtcgtat tggcgaaggc gagcaaccgg
     5461 ccctgggtgg tgttgccgat gccgcgcttg ggggaattga cgatccgcgt gaaggccacc
     5521 gagtcgcgcg ggtttgcgag gtactggaga taggcgatcg cgtccttgat ctcggcccgg
     5581 tcatagaagc gggtgccgcc gatcacccgg taggcggtgc cgaaccggac cagcgtgtct
     5641 tcgagaaccc ggctcatcgc gttcatccgg tagaagaccg ccacgtcgag cggcgagatg
     5701 ccctcgtcct cgaccagccg ttcgatctcg cccgccaccc agcgggcttc ctcatgctcg
     5761 tcagagagcc gcacgacctc tatttcgtcg ccctggccga ggtcggtcca gagcttcttc
     5821 gggcgccggt cgcggttgtt ctcgaccacc gcgttggcgg cggaaaggat ggtctgggtc
     5881 gaacggtaat tctgttcgag cttgaccacc ttcgcgtccg gaaagtcctc accgaagtcg
     5941 aggatgttgc ggatgtcggc gtggcggaag ccgtagatcg actggtcctc gtcgccgacc
     6001 acggtcaggt tccggtgctc gccgcagagc agctgaagca gcctgtactg ggcgtggttg
     6061 gtgtcctggt attcgtcgac cagcacgtag cggaaggccc gccgccaacg ctccctcacc
     6121 tcgggaaaca gttccagcac gttgaccatg cggaccagga ggtcatcgaa gtccatcgcg
     6181 ttggactcga ccatgcgctg ctcgtagagc gcgtacactc ccgccgccgt gtcctcgaag
     6241 tagg
//
LOCUS       DAOQFI010000016         6167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_4802_length_6167_cov_3.076898, whole genome shotgun sequence.
ACCESSION   DAOQFI010000016 DAOQFI010000000
VERSION     DAOQFI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4802_length_6167_cov_3.076898"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLE93_00595"
     CDS             <1..945
                     /locus_tag="PLE93_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85573.1"
                     /translation="DLSPWLLSSSGDMGGKLIGQPLNVYPENNLTPGNNLSGNQFWSP
                     NSQDVLLQERTRTIPATTLSEHVQQKGLTPNRIAIAHIERPAGKKLKVVSSEVGNWAV
                     APTQYFPSVGSNTSVTVNGKAGGTADLVYTGNLQTGSWSSEYHDYTKDGKTFVNGTYF
                     VSRTIEGVWTLKADVTVTGQHTGRLNMDMTIQGGDPLPIMTGGITAVYDGKKAPALPK
                     LGACYKKLPQKSRLAAQVKSKRAGKATKVTVTVTANVYGDRRPVQNAKVKLAGRIVRT
                     DRSGKAKLKVRGRGTRKVLVTAGDTFIKTSKVAHLKQR"
     gene            1047..2207
                     /locus_tag="PLE93_00600"
     CDS             1047..2207
                     /locus_tag="PLE93_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH85574.1"
                     /translation="MPVGYLITLFLLGTCVLLLLAPVRKRPLGLVSFWGGLLVNEQAH
                     WFAVLLAGSTVLAFVEGDISGVARAIAGLAVLAEAGLIVLFLRSFRSRGTLREALAAF
                     DSDSPAALAGRRSYLAALAAPPAMGRSRVRLKSDIPYGPVPGRANRLDLYLPRHGKVT
                     GPTMIYMHGGRFRSGNKRREAQAIKCQLARRGWSVISANYRLNPEGSFPDYLIDLKRV
                     IAWARGEGQAFGIDPERIFLAGGSAGAHIAAMAGLTAGKPELQPGFEQVNTAVSGVIG
                     IYGYYGGLESPGGRPPAVPSSPRRHVQPGAPPFLLIHGDHDSVVGRGGAAALVKELQG
                     AGNQASLAVLPGAEHTFDLLRSVRTENTIDSIVDFAAWAERSENRDLSQSRR"
     gene            2219..2977
                     /locus_tag="PLE93_00605"
     CDS             2219..2977
                     /locus_tag="PLE93_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85575.1"
                     /translation="MGAKGAKGNLPGGLRTGLVGLGLLALAITCLLTSPAPDRSNAET
                     RANAEPQGGILPCGSHVVLTAVYRNRDGRIHFEGVADPKFAGRQARIYDRDNHLVAAT
                     TVGEDGIFWANANSKDEGYTWLTRFIARVGGSESRWKRIGQAVGLRDRQPFELEPTAR
                     NHGKTLERTRVRVKVAGGHSNLLVVGLQVGCSRFDVIELASTYTDSNGVSDLNLPRPP
                     ADRPYWIFRVSTEDDWKISPPIVVKSVPESGPTG"
     gene            3050..4714
                     /locus_tag="PLE93_00610"
     CDS             3050..4714
                     /locus_tag="PLE93_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNH85576.1"
                     /translation="MTQEQLEAYHEYTRKHGVNRGLYLFARIVLQPFFLIYLRLSRQG
                     RGNAQIKGATIVAANHRSFLDPFVIGTCLPWNKPMNYVAKVELFEKRWQGFLLCRVGA
                     FPIRRGEADELAIETAELVLARGGTICIFPEGTRIRKGSLGKPKRGVGRLALRSGAPV
                     LPVAVTGSENVRRGWQIRPRKVDIRVGRPMSFPQSESPSPALAASVTDRIWPNVELQW
                     EDIGGLPPMRRAAVIGAGSWGTAVAVLLARGGLEVQLGTRTADQAEEMALARENTRYL
                     PGVRLPDGIEVRASSQIELGGVDLLCIAVPSASYPQAVGAVADRVGRRAGVLLLAKGL
                     IAPKGQLPSEYVGERIRSRGVSCLGGPAHAKEAVSGSAALVLGSADGDLRAQLGDVFD
                     RAGLVCERSRDVVGVEMAGAAKNAAVLAAAAAEPHGLNAAGIAAAAVWDECVGYAVKH
                     GADLESFNGLAGVGDLLATVMASGSRNRRAGELLGKGSAPDQIRETIGQASEALDSVP
                     QIAATVAADGCPAEALTGLADLATGRISSDEWVAALRRSGHSRRAA"
     gene            4760..5326
                     /locus_tag="PLE93_00615"
     CDS             4760..5326
                     /locus_tag="PLE93_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH85577.1"
                     /translation="MSGPDAKSTPESKAEIDRAFEELYRDHLKDVYSYCYYRIGNHHD
                     AEDLTEQTFLQAYRHFARARRESDGRPLRPWLIRIAHNLAANYYRDRSRKPQTPLESI
                     EPPAAKHSTEDLVEGRAELRAVIENLQDLPDDRREALVMRFALGMDNREIARAMGKSD
                     GATKVLIHRAIKQLQESLEAEGVSVEHG"
     gene            5319..5600
                     /locus_tag="PLE93_00620"
     CDS             5319..5600
                     /locus_tag="PLE93_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85578.1"
                     /translation="MDEIRIENLLADALKPVEPPQRMSDRLHDTFSAITDAAASDLSD
                     WAEELTESELESLRDPRNWIRPVTALAAGGVATGALILVGFRRRSRYRG"
     gene            complement(5633..6139)
                     /locus_tag="PLE93_00625"
     CDS             complement(5633..6139)
                     /locus_tag="PLE93_00625"
                     /inference="COORDINATES: protein motif:HMM:NF025683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HNH85579.1"
                     /translation="MERTAYARLYATVTGAFLVLLGFVSLLSNTEFQARELTSELLGF
                     YAINGWSGVFHVAAGLLGLLLARPLPRLYALLAGIVFTGLGIWGILAANGTWLLGGLP
                     ANRWANLVNLLIGLLGIIAYAASRWDRITAFTGSLGSRFEKMAENRRQKRRRRKIRKR
                     RTATKSRA"
BASE COUNT         1047 a         2060 c         2043 g         1017 t
ORIGIN      
        1 gacctctcgc cctggttgct ctcctcgtcc ggcgacatgg gcggcaagct gatcggccaa
       61 ccgctgaacg tctacccgga aaacaacctg accccgggca acaacctttc gggcaaccag
      121 ttctggagcc cgaacagcca ggacgtcctg ctgcaggagc gcacccggac aatcccggcg
      181 accacgctct cggagcatgt tcagcagaag ggcctgactc cgaaccggat cgcgatcgcc
      241 cacatcgagc gtccggccgg caagaagctg aaggtcgttt cctcggaggt cggcaactgg
      301 gcggtcgctc ccacccagta cttcccctcg gtcgggtcga acacctcggt caccgtgaac
      361 ggcaaggccg gcggcaccgc cgacctggtc tacaccggga atctccagac cggaagctgg
      421 tcctccgagt accacgacta caccaaggac ggcaagacct tcgtcaacgg gacctacttc
      481 gtcagccgca ccatcgaagg ggtctggacc ctcaaggccg atgtcacggt gaccggccag
      541 cacacgggcc ggctcaacat ggacatgacg atccagggcg gcgacccgct gccgatcatg
      601 accggcggca tcactgctgt ctatgacggc aagaaggctc cggccctgcc caagctgggc
      661 gcctgctaca agaaactccc gcagaagagc aggctcgccg cccaagtcaa gtcgaagcgc
      721 gccggcaagg cgaccaaggt gaccgtaacc gtcaccgcca acgtctacgg cgacaggcga
      781 ccggtccaga acgcgaaggt gaagctcgcc ggccggatcg tccgcaccga ccgctcgggc
      841 aaggcgaagc tcaaggtccg cggccgcggc acgcggaaag tgttggtcac cgccggggac
      901 accttcatca agacctcgaa ggtcgcgcac ctgaagcagc gctgaccagg caatcctcgt
      961 cccttccggg tcctgtcggg caacgggccg gccgattatg gccggcccgt ttttcgtggg
     1021 aggaagccgg caactagctt gtgggaatgc ccgtcggcta cctgatcacg ctttttctgc
     1081 tcggaacctg cgtgctcctt ctgctggctc cggtgcggaa gcgaccactc ggcctggtct
     1141 ccttctgggg cgggctcctg gtcaacgagc aggcgcactg gttcgccgtg ctgcttgccg
     1201 gttcgaccgt gctggccttc gtcgagggcg acatttccgg tgtcgcccgg gcgatcgcgg
     1261 ggcttgccgt actggccgag gccggactga tcgtcctctt tctcaggtcg tttcggtccc
     1321 gcgggacctt gcgggaagcg ctggccgctt tcgattcaga ttcacccgcc gcacttgcag
     1381 gccggcgttc atacctggcg gcgcttgccg ctccgccggc gatgggccga agtcgggttc
     1441 ggctgaaatc cgacattccc tacggaccgg tcccgggccg ggccaatcgg ctcgatctgt
     1501 acctgccccg gcacggcaag gtgaccggcc cgaccatgat ctatatgcac gggggcaggt
     1561 tccggtcggg taacaagcga agggaggccc aggcgatcaa gtgccagctg gcccgccgcg
     1621 gctggtcggt gatcagcgcc aactaccggt tgaatcccga aggatcgttt cccgactacc
     1681 tgatcgatct caaacgcgtg atcgcctggg cccgcgggga ggggcaggca ttcggcatag
     1741 atccggagcg gatctttctg gctggcggct cggccggggc tcacatcgcg gcgatggccg
     1801 ggctgaccgc gggcaagccg gagctgcaac cgggattcga gcaggtgaac acggccgtct
     1861 ccggggtgat cgggatctac ggctactacg gcggccttga atcacccggc ggccgccccc
     1921 cggctgtccc ctccagcccc cggcgccacg tgcagccggg cgcacccccg tttctgctca
     1981 tccacggaga ccacgacagc gtggtcgggc ggggcggagc cgcggccctc gtcaaggaac
     2041 tccagggcgc cggcaaccag gcgtcactgg ccgtcctgcc cggggccgaa cacaccttcg
     2101 acctgctgcg ctcggttcgc accgaaaaca cgattgactc gatcgtcgat tttgccgcct
     2161 gggcagaacg ttcggaaaat cgtgatcttt cacaatcccg gcgctaagat gggctgttgt
     2221 gggggcgaag ggagcaaagg ggaatcttcc cggcgggctg cggaccggcc tggtcggtct
     2281 ggggctgctt gcgctcgcga ttacctgtct cctgacttcg cctgcgcccg atagatcgaa
     2341 cgccgagact cgggccaacg ctgagccgca agggggcatc cttccctgcg gcagccacgt
     2401 ggtccttacc gcggtctatc gcaacaggga cgggcggatc catttcgaag gggtcgccga
     2461 tccgaaattc gccggcaggc aggcccggat ctatgaccgg gacaaccatc tggtcgcagc
     2521 gacgaccgtg ggcgaggacg gaatcttctg ggcgaacgcc aattcaaagg acgagggtta
     2581 cacctggctc acccggttca tcgccagggt gggtggtagc gagtcccgct ggaaacggat
     2641 cggccaggcg gtcggcctca gggaccggca gccctttgaa ctggagccga cggcacgcaa
     2701 ccacggcaag accctggagc gcaccagggt cagggtcaag gtggccggcg gccacagcaa
     2761 tctcctggtg gtcggtctgc aggtcggctg ttcccgattc gacgtgatcg aactcgcttc
     2821 gacctacacg gactcgaacg gggtgtccga cctgaacctg ccccgtcccc cggcggatcg
     2881 tccctactgg atcttccgag tcagcaccga agacgactgg aagatctcgc ccccgatcgt
     2941 cgtcaaatcg gtgccggaat ccggcccgac cggctgattc gcccgggccc gcccgaaaac
     3001 ggcgaggacc cgttgtaacc gggtccgggg gtttgtgtag gactcttttg tgactcagga
     3061 gcaactcgag gcctaccacg agtacacgcg caagcacggg gtgaaccgcg gtctttacct
     3121 cttcgcgcgc atcgttctgc agccgttttt cctaatctac ctgcggcttt cgcgtcaggg
     3181 caggggtaac gcgcagatca agggcgcgac catcgtcgcc gccaatcacc gcagcttcct
     3241 cgacccgttc gtgatcggca cctgcctgcc ctggaacaag ccgatgaatt acgtggcgaa
     3301 ggtggaactg ttcgagaagc gctggcaggg gttcctcctc tgccgggtcg gggccttccc
     3361 gattcgccgg ggagaggctg acgagctggc gatcgaaacc gccgagctgg ttctggcccg
     3421 gggcgggacg atctgcatct ttcccgaagg cacccggatc aggaagggtt cgctcggcaa
     3481 gccgaagcgg ggcgtcgggc gtctcgctct ccggagcggg gcaccggttc tgccggtcgc
     3541 cgtcaccggt tccgagaacg tgcggcgcgg ctggcagatc cggccgcgca aggtcgacat
     3601 ccgggtcggc cggccgatgt cctttcccca gtcggaatcg ccttctccgg ccctcgccgc
     3661 atcggtcacc gatcgcatct ggccgaacgt cgaacttcaa tgggaagaca tcggcggtct
     3721 gccgccgatg cgccgggccg cggtcatcgg agccggcagc tggggcaccg cggtcgcggt
     3781 gctgctggcc cgcggcgggc tcgaagtcca gctcggcacc cgcaccgccg accaggcgga
     3841 ggagatggcc ctcgcgcgcg agaacaccag gtatctgccc ggcgttcgcc tgcccgacgg
     3901 catcgaagtc cgggcctcca gccagatcga actcggtgga gtcgacctgc tctgcatcgc
     3961 ggtcccgtca gcttcctacc cgcaggcggt cggagcggtc gccgaccggg tcggtcgccg
     4021 ggccggggta ctgcttctcg ccaaggggtt gatagccccc aagggtcaac tgccatccga
     4081 atacgtgggt gagcggatcc gctcacgcgg cgtctcctgc cttggtggtc ccgcacacgc
     4141 aaaggaagcg gtgtccggtt cggccgctct ggttcttggc agcgccgacg gtgaccttcg
     4201 ggctcaactc ggtgacgtgt tcgaccgggc cggtctggtc tgcgagcgct cgcgggacgt
     4261 ggtcggggtg gagatggccg gggcggcgaa gaatgccgcc gtgctggccg ccgctgcggc
     4321 cgagccacat ggcctgaatg cggccgggat cgccgccgcc gcggtctggg atgaatgcgt
     4381 cggttacgcc gtaaaacatg gggcggacct tgaatccttc aacggcctcg ccggggtcgg
     4441 agacctgctt gccacggtga tggcatccgg cagtcgcaat cgccgggcgg gtgaactgct
     4501 cggcaagggc agcgccccgg accagatccg cgaaacgatc ggccaggctt cggaagctct
     4561 cgattcggtc ccccagatcg ccgccaccgt ggcggccgac ggctgccccg ccgaggccct
     4621 gaccggcctc gcggatctcg ccaccgggcg aatcagctcc gatgaatggg ttgcggcgct
     4681 ccgccgctcc gggcattcga ggcgggcggc atgagcacgc ccgggtcgga ggtcgccggt
     4741 ttcgggataa ggtgcgggcg tgtccggccc cgacgcgaaa tcaactccgg agtcgaaggc
     4801 cgagatcgat cgggccttcg aggagctcta ccgcgatcac ctcaaggacg tctactccta
     4861 ctgctactac cggatcggca atcaccacga cgccgaggat ctgaccgagc agacatttct
     4921 tcaggcctac cggcactttg cccgggcccg gcgggaatcg gacggtcgcc cgcttcgccc
     4981 ctggctgatc cggatcgccc acaacctcgc tgcgaactac taccgcgatc gttcgcggaa
     5041 accgcagact ccgctggaga gcatcgagcc gccggccgcg aagcactcga ccgaggatct
     5101 ggtcgagggt cgggccgaac tccgggcggt gatcgagaac cttcaggatc tgccagacga
     5161 tcgtcgcgag gctctggtca tgcgtttcgc gctcggcatg gacaaccgcg aaatcgcccg
     5221 cgcgatgggc aaatccgacg gggccaccaa ggtgctgatt catcgcgcga tcaagcagct
     5281 gcaggaatcg ctggaagcgg aaggagtttc cgttgagcat ggatgagatc aggatcgaga
     5341 acctgctcgc cgatgcgctg aagccggtcg agccgccaca gcgcatgtct gatcgacttc
     5401 acgacacctt ctcggcgatc accgacgccg ccgcttcgga cctttccgat tgggccgaag
     5461 aactgaccga aagtgaactc gagtcgctgc gtgacccccg caactggatc cgtccggtca
     5521 ccgccctcgc cgcgggcggg gtagctaccg gcgccctgat cctggtcggc ttccgccgtc
     5581 gaagccgcta ccgcggctga cacatcaccc ttgtgaccag ccgcttccgc ggctaggcgc
     5641 ggctcttggt tgctgtccgg cgtttgcgga tcttgcgtcg cctgcgcttc tggcggcgat
     5701 tttccgccat cttttcgaac cggcttccga ggctgccggt gaacgcggtg atccggtccc
     5761 agcggctggc cgcgtaggcg atgatgccga gcaggccgat cagcaggttg accaggtttg
     5821 cccaccggtt ggctggcagc ccaccgagca gccaggttcc gttggcggcg aggatgcccc
     5881 agatgccaag gccggtaaag acgattccgg ccagcagcgc gtagaggcga ggaagggggc
     5941 gggcgagcag caggccgagt agtccggcgg cgacgtgaaa gacaccggac cagccgttga
     6001 tcgcgtagaa gcccaacagc tcgctggtca gctcgcgggc ctgaaactcg gtgttgctga
     6061 ggaggctgac gaaaccaagc agcacgagga aggcgccggt caccgtcgcg tacagccggg
     6121 cgtatgccgt cctctccatc gaccgccatt ctcacaggaa cgccgga
//
LOCUS       DAOQFI010000017         6125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_4866_length_6125_cov_3.064745, whole genome shotgun sequence.
ACCESSION   DAOQFI010000017 DAOQFI010000000
VERSION     DAOQFI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4866_length_6125_cov_3.064745"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PLE93_00630"
     CDS             <1..1065
                     /locus_tag="PLE93_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNH85580.1"
                     /translation="VAAMMGPCAAGTAYIPGLSDFVPMVVGQGAMALAGPHLVKAVTG
                     EEIEMEDLGGAKVHCRKSGVGDLEVKDDPECIESIKRYLSYFPSNCEQKPPVTETADP
                     EDRGSEKLLEIIPDSPRTPYNMYEVIEEIVDDGDWFDIKPKFAKSLITCLARMGGQPV
                     GIVANQPKVLGGVLDVDSADKAARFINLCDAFNIPLLFLQDVPGFLVGSKVEQQGIIR
                     HGAKMLYAVSRATVPKITVLVRKAYGAGYYVMCGRAYEPDLIVAWPGAEISVMGAEGA
                     VNIIGRSAIEAADDPEATREAMLNAVRQQIDPYIAAGNAMIDDIIDPRETRQTVIRGL
                     RMAKDKHVERPWKKHGVMPV"
     gene            1062..1397
                     /locus_tag="PLE93_00635"
     CDS             1062..1397
                     /locus_tag="PLE93_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85581.1"
                     /translation="MSEVIEVTIPHEQVQLNGVIRFDGSVVEIFGFNDVHSVRMHIQQ
                     IEFAEVKFDKGLLAQPRIMFKGKWSNLGYNQAIDPPDQIKPELENFVNAINAAIGNDA
                     ANYPNGETQ"
     gene            1394..2272
                     /locus_tag="PLE93_00640"
     CDS             1394..2272
                     /locus_tag="PLE93_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNH85582.1"
                     /translation="MSKFEDPVIISNSISGVIANRDQCPAIPYTPEEYAAEARRSVDE
                     GASQIHIHARTPDGTPSYEIEDFQAITEAIKAECPEVIINYSTGAIGVPIEKRIAYLE
                     ACRPDVAPLNMSSMNYAKYSKRRKDFVFKAVFENSFDTIVEFITAMNDLGIKPEHECF
                     DAGHVANLDPLYDMELIKPPSQISLVMGVTGGIRPTPRNVAVMADQVPGGPEGPNNWQ
                     VIGISRDQWKLLAAAITLGGNVRAGVEDNLYLPDGTMCKSNGELIGKARQMVEDAGRK
                     VATIAEAREILGLKVA"
     gene            2269..3501
                     /locus_tag="PLE93_00645"
     CDS             2269..3501
                     /locus_tag="PLE93_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNH85583.1"
                     /translation="MSLPLEGIRVLDLTRLLPGGFCSQMLVDFGAEVIKVEDTGPGDY
                     IRYAPPLYESDADPAEATASGLYLSLNRGKRSIRLDLKSEAGREAFYRLLPTADVVLE
                     GFRPGVAAKLGVDYETLSRKNEGLVYCSISGYGQDGPSRDRAGHDINYLGSTGMLAMT
                     GQAGGPPVQPAGQIGDLGGGAMNAAFGILAALIEKGRSGKGQMVDISMTDGAFAWLAM
                     AAGAYFAGGEVPRRGEVMLGGGIVCYLTYEAADGWVSCGALEPKFWCAFCEGTGHEDL
                     IEIQFERPGGEAWEKVAAVFRERTRDEWKSFNDQYDCCIEPILELDEAVESDLLRERG
                     MIVEWEQPGIGPVRQIGSPIKMSRTPAAEPGPAPAIGADTREVLASAGLSAEEIEGLF
                     ESGVAAGPGSGGEMEFRA"
     gene            3498..4211
                     /locus_tag="PLE93_00650"
     CDS             3498..4211
                     /locus_tag="PLE93_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNH85584.1"
                     /translation="MTQAAAEEKPDAPAEQLLKMSGLVEASGVPAPTIKHYLREGLLP
                     EPVRTSRNMAWYRPETVERIRLIKRLQEERYLPLKAIRALFEEDPAQAEAMLEVEDRI
                     LERALAGERTRTSATEVRKYGVPKKVLERLAEIGILTPNSRGYSPSDVEIIEAMSRFR
                     AGGYDEQIGFTVYDTLRYKHALEALVKEEVDVLMERLEGEITPEHAVELIEAGAEPLK
                     DLIAALHTKLMVEEVERRR"
     gene            4248..>6125
                     /locus_tag="PLE93_00655"
     CDS             4248..>6125
                     /locus_tag="PLE93_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNH85585.1"
                     /translation="METGGVSKGDVLRSLGAAALLLLAALLFAPPAAAALTARDSDPV
                     VLKGSQAGNLAGIDPGRLVAFRWTGSAWDQIPLQVDERKTIDMQSLYPWVTGGANQGY
                     VTNYLVPVNVEVYADPDTRTGADPDATLDPNDEFVFMAFDSGSKAPIDSDPAHVVPRS
                     GVELELDDPLNGDSSFVYLFRSDGNLSPGAGRQYVTYSPSLQNGIGSSYRDNYRYFFP
                     LNSNPPANGYNNENTTLTTPSYSVHSIDRWIDDQLRITSGSASGADILDRDKVAFTPG
                     YCGRTEDTFTGRENFNSSDNAEGAFVTNRAGPVRAIRSYIGANSGPYTQREQIFYRDR
                     QDSRTYLRVHSIPYVMSFTDYSPAASGMTWRSSINTTGVTVDGTPDLFTVPGFTDPRD
                     LTGIQYPSAGAGVSGWEQITGTQGTVDAVSRLVTDIPNVAVTAYYLDNSSPSTTFNQS
                     QMTGDPTYQCTGDAFAYGASGLQLKVATPIGTDLPNTDPRAGGSENEVTMERSIFYGP
                     PGGNSVEAESRRQQVDNPIELTSRRHIRPPDGPPDVSPASRDFGTLEISESPGASQQF
                     TIHNAGNYELSLSQAVVSGGAGFEIDGDSCSNQTLPAGMSCSIGIRFDPASAGAVTAK
                     AG"
BASE COUNT         1171 a         2025 c         1981 g          948 t
ORIGIN      
        1 gttgccgcga tgatgggtcc ctgcgccgcc ggcacggcct acatccccgg tctttcggac
       61 ttcgtgccga tggtggtcgg gcagggcgcg atggccctgg ccggtccgca ccttgtcaag
      121 gccgtcaccg gcgaggagat cgagatggag gacctcggcg gggccaaggt tcactgccgc
      181 aagtccgggg tcggagacct cgaggtgaag gacgacccgg aatgcatcga gtcgatcaag
      241 aggtacctga gctacttccc gagcaactgc gagcagaagc ccccggttac cgaaacggct
      301 gatccggagg accgcggctc ggagaagctc ctggagatca tccccgattc gcctcgcacc
      361 ccgtacaaca tgtacgaggt gatcgaggag atcgtcgatg acggtgactg gtttgacatc
      421 aagccgaagt tcgccaagtc gctgatcacc tgcctcgccc ggatgggcgg ccagccggtc
      481 ggcatcgtcg ccaaccagcc caaggtactg ggcggcgtgc tcgacgtcga ttccgccgac
      541 aaggcggccc gcttcatcaa cctttgcgac gccttcaaca tcccgctgct cttcctgcag
      601 gacgtgcccg gcttcctggt cggctcgaag gtcgagcagc aggggatcat ccgccacggg
      661 gccaagatgc tctacgcggt cagccgcgcc accgtgccga agatcaccgt gctggtccgc
      721 aaggcttacg gcgccggcta ctacgtgatg tgcggccggg cctacgagcc cgacctgatc
      781 gtcgcctggc ccggagccga gatctcggtg atgggggccg agggcgcggt caacatcatt
      841 ggccgctcgg cgatcgaggc ggccgatgat ccggaggcga cccgcgaggc gatgctgaac
      901 gcggtccgcc agcagatcga cccctacatc gcggccggca acgcgatgat cgacgacatc
      961 atcgacccgc gcgaaacgcg gcagaccgtg atccgcggtc tccgcatggc aaaggacaag
     1021 catgtcgagc gtccctggaa gaagcacggg gtaatgccgg tatgagcgag gtgatcgagg
     1081 tcacgattcc ccacgagcag gttcagctga acggcgtgat ccgcttcgac ggctcggtgg
     1141 tcgagatctt cggcttcaac gacgtccact cggtccggat gcacatccag cagatcgagt
     1201 ttgccgaggt gaaattcgac aagggcctgc tggcccagcc gcgcatcatg ttcaagggca
     1261 agtggtcgaa cctcggctac aaccaggcga tcgatccacc cgaccagatc aagcccgaac
     1321 tcgagaactt cgtcaacgcg atcaacgccg cgatcggaaa cgacgccgcg aactacccga
     1381 atggagaaac gcagtgagca agttcgaaga cccggtgatc atcagcaact cgatctccgg
     1441 ggtgatcgcc aaccgggacc agtgcccggc gattccgtac acccccgagg agtacgcggc
     1501 cgaggcgcgc cgctcggttg acgagggtgc cagccagatc cacatccacg cccgcacccc
     1561 ggacggcacg ccctcgtacg agatcgagga cttccaggcg atcaccgagg cgatcaaggc
     1621 cgagtgcccg gaggtgatca tcaactactc gaccggcgcg atcggcgtgc cgatcgagaa
     1681 gcggatcgcc tacctcgagg cctgccgccc cgatgtcgcc ccgctcaaca tgagctcgat
     1741 gaactacgcc aagtactcga agcgccggaa ggacttcgtc ttcaaggcgg tcttcgagaa
     1801 ctccttcgac acgatcgtcg agttcatcac cgcgatgaac gacctcggca tcaagccgga
     1861 gcacgagtgc tttgacgccg gtcacgtcgc caacctcgat ccgctctatg acatggaact
     1921 gatcaagccg ccctcccaga tctccctggt catgggtgtg accggcggca tccggccgac
     1981 cccgcgcaac gtggcggtca tggccgacca ggtgccgggc ggccccgagg gcccgaacaa
     2041 ctggcaggtg atcggcattt cccgcgacca gtggaagctg ctggccgctg cgatcaccct
     2101 cggcggcaat gtgcgggccg gggtggagga caacctctac ctgcccgacg gcaccatgtg
     2161 caagtcaaac ggggagttga tcggcaaggc ccggcagatg gtcgaggacg ccggccgcaa
     2221 ggtcgcgacg atcgccgaag cccgcgagat actcggcctg aaagtggcat gagtctgcca
     2281 cttgaaggaa taagggtcct cgacctgacc cggcttctgc cgggcggctt ctgctcgcag
     2341 atgctggtcg acttcggggc cgaggtgatc aaggtcgagg acaccggccc cggtgactac
     2401 atccgctacg ccccgccgct ttacgaaagc gatgccgacc ccgccgaggc gacggcttcc
     2461 ggcctctacc tgtcactcaa ccggggcaag cgctcgatcc gtctcgacct caaaagcgag
     2521 gcgggccgcg aagccttcta ccgcctcctg ccgaccgccg acgtggtgct cgaaggcttc
     2581 cggcctggcg tggccgccaa gctcggggtc gattacgaaa ccctgagccg aaagaacgag
     2641 ggcctcgtct attgctcgat cagcggctac ggccaggacg gcccgtcacg cgaccgggcc
     2701 ggccatgaca tcaactacct cggttcgacc ggcatgctgg cgatgaccgg ccaggccggg
     2761 ggcccgccgg tccagccggc cggccagatc ggcgacctcg gtggcggcgc gatgaacgcc
     2821 gccttcggga tcctcgccgc gctgatcgaa aagggccgaa gcggcaaggg ccagatggtc
     2881 gacatctcga tgaccgacgg agccttcgcc tggctggcga tggcggccgg cgcctacttc
     2941 gccgggggcg aggtaccacg gcggggagag gtgatgctgg gcggcggcat cgtctgttac
     3001 ctcacctacg aggcggccga cggctgggtc agctgcggcg cgctcgagcc caagttctgg
     3061 tgcgccttct gtgaaggcac gggccacgag gacctgatcg agatccagtt tgaacggccc
     3121 gggggcgagg cctgggagaa ggttgctgcg gtattccgcg agcggacccg ggacgagtgg
     3181 aagtcattca acgaccagta cgactgctgc atcgaaccga tcctcgaact cgatgaagcg
     3241 gtcgagtcgg atctgctccg cgaacgcgga atgatcgtcg agtgggaaca gccgggcatc
     3301 ggaccggtca ggcagatcgg ttcgccgatc aagatgagtc gcaccccggc cgcagaacct
     3361 ggccccgccc cggcgatcgg tgccgacacc cgcgaggtgc tggcctcggc cggactgagc
     3421 gcggaggaga tcgagggcct gttcgaatcc ggtgtcgccg ccgggcccgg gagcggggga
     3481 gagatggagt tccgggcgtg acccaggcgg cagccgaaga gaagccggac gcgccggccg
     3541 aacagcttct gaagatgtcc gggctggttg aagcctcggg cgtcccggcc ccgaccatca
     3601 agcattacct gcgggagggt ctcctgccgg agccggtccg gacttcccgc aacatggcct
     3661 ggtaccggcc ggaaacggtc gaacgaatcc gcctgatcaa gcgcctgcag gaggagcggt
     3721 acctgccgct gaaggcgatc cgggcgctct tcgaggagga cccggcgcag gccgaggcga
     3781 tgctggaggt cgaggaccgg attctcgagc gcgccctggc cggcgagcgg acccgaacca
     3841 gtgcgaccga agtcaggaag tacggcgtgc cgaagaaggt tctggagcgg ctggccgaga
     3901 tcggcatcct gaccccgaac tcgcgcggct actcgccctc ggacgtggag atcatcgagg
     3961 cgatgtcacg cttccgggcc ggcggctacg acgaacagat cggcttcacg gtctatgaca
     4021 ccctgcgtta caagcatgcc ctcgaagccc tggtcaagga ggaggtcgac gtgctgatgg
     4081 aacggctcga aggcgagatc acgccagaac acgcggtcga gctgatcgaa gccggggccg
     4141 aaccgctcaa ggacctgatc gccgccctgc acacgaaact gatggtcgaa gaggtcgaac
     4201 gccgccgctg agcggttttg cccggaaccg gcggtaccct tcccttgatg gaaacagggg
     4261 gagtttcaaa gggagatgtg ctgcggtcgc ttggggcggc cgctctgctg ctgctcgcag
     4321 cgctgctttt cgctccgcct gcggctgcgg cgctgaccgc ccgcgattcc gatccggtcg
     4381 ttttgaaagg cagccaggcc gggaacctgg ccgggatcga tccggggcgg ctcgtcgcct
     4441 tccgctggac cggatctgcc tgggaccaga tcccgctgca ggtcgatgaa cggaagacga
     4501 tcgacatgca gagtctctat ccgtgggtca ccggtggcgc caaccaggga tacgtgacca
     4561 attacctggt cccggtgaac gtcgaggtgt acgcggaccc cgacacccgg accggcgccg
     4621 accctgacgc caccctcgac ccgaatgacg agttcgtctt catggccttc gactcggggt
     4681 cgaaggcgcc gatcgattcc gatccggccc acgtggttcc ccgctcgggg gtggagctcg
     4741 aactcgacga tcctctgaac ggagactcct cgttcgtcta cctgttccgg tccgacggca
     4801 acctcagccc cggtgccggc aggcagtatg tgacctattc gccttccctc cagaacggga
     4861 tcggctcgag ttacagggac aactaccgct acttcttccc gctcaactcc aacccgccgg
     4921 ccaacggcta caacaacgag aacacgaccc tcaccacccc gagctactcg gtccattcga
     4981 tcgaccgctg gatcgatgac cagctcagaa tcacctccgg ctcggccagc ggggcggaca
     5041 ttctcgatcg cgacaaggtc gccttcaccc ccggctactg tggtcgcacc gaagacacct
     5101 tcaccggccg tgagaacttc aacagcagcg acaacgccga aggcgccttc gtcaccaacc
     5161 gtgccggccc ggtccgggcg atccgttcat acatcggcgc caacagcggc ccgtacacgc
     5221 agcgtgaaca gatcttctac cgcgatcgcc aggattcccg gacctatctg cgggttcact
     5281 cgatcccgta tgtcatgtcg ttcaccgact actcgccggc tgcatccggg atgacctggc
     5341 gttcatcgat caatacgacc ggggtgaccg tggacggcac gcccgatctg ttcaccgtgc
     5401 ccggattcac cgacccccgg gacctgaccg gcatccagta cccgtccgcg ggcgcgggag
     5461 tgagcggctg ggaacaaatc accggcacgc agggaacggt cgacgcagtc tcgcggctgg
     5521 tcaccgatat cccgaatgtc gcggtcaccg cctattacct cgacaacagc agccccagca
     5581 ccaccttcaa ccagagccag atgaccgggg atccgaccta tcagtgcaca ggggatgcgt
     5641 tcgcctacgg cgcaagcggg cttcaactca aggtcgcgac cccgatcggg accgacttgc
     5701 ccaacaccga cccgagagcg ggcggcagtg agaacgaagt gaccatggag cgctcgatct
     5761 tctacggacc gcccggtggc aattccgtcg aggcagagag caggagacag caagtcgaca
     5821 atccgatcga actgacttcc aggcgccaca tccgaccccc ggacggaccg cccgatgtgt
     5881 cgccggcgag ccgggacttc ggcaccctgg agatcagcga aagcccgggc gcgagccagc
     5941 agttcacgat ccacaacgcc ggcaattacg agctgtcgct gagtcaggcc gtggtcagtg
     6001 gcggagccgg gttcgaaatc gacggtgaca gctgttcgaa ccagaccctg ccggccggaa
     6061 tgagttgcag catcgggata cggttcgacc cggcttcggc aggtgcggtc actgccaaag
     6121 ccggg
//
LOCUS       DAOQFI010000018         6049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_4987_length_6049_cov_2.968302, whole genome shotgun sequence.
ACCESSION   DAOQFI010000018 DAOQFI010000000
VERSION     DAOQFI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4987_length_6049_cov_2.968302"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLE93_00660"
     CDS             complement(<1..297)
                     /locus_tag="PLE93_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNH85586.1"
                     /translation="MPPRTILYTGKGGVGKTSVAAATARRCAAEGRRTVILSTDPAHS
                     LSDSLGVELGPDPVEVSPGLWGQEVLAEAEMRRNWDRVAAWMSGLLQSKGVDQVR"
     gene            327..947
                     /locus_tag="PLE93_00665"
     CDS             327..947
                     /locus_tag="PLE93_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85587.1"
                     /translation="MERTRIPLVLVLVLAFSLVMQACGSSDPDPATILDRALTREHLS
                     SFGNGSGGPTGGIVSVEALGYEDRVLDERKVAADPQVMSDIRSALGSDSGLRGLVGDL
                     RYDGTEEVSGVGTDHISGELDVAGLARALEAAGGGEVGQIAGVKTGSSLEDSLVSADF
                     DLYAGQQDGDIRRLDLTLALDDPDNALPPARIRFSLTPRPSGANQT"
     gene            966..1994
                     /locus_tag="PLE93_00670"
     CDS             966..1994
                     /locus_tag="PLE93_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNH85588.1"
                     /translation="MEYLLLLFSFAIILTGALLFTNAIEWLGHKLNLGEGAVGSILAA
                     VGTAMPETLIPIVAIIGGVSGSDEVAVGAIVGAPFLLATIAMALVGISALAYIKRRPQ
                     GKHLDVHVETLDRDLVFFMIFFTLALVVGVVDVPQSVQIGGGVICLLAYVYYVVQTIR
                     NSGDVAESHEMDPLIMDRRTDRTGGPPNSIMIIQLLVGLASMVGGAHLFVHELLSVAE
                     HLGVSAIILSLILAPLATELPEKANSFFWVRDGKDALALGNITGAMVFQSTIPVAIGM
                     IFIDWSLDKYAVLSMLLGLAGGVVAYWSLRRNGQFNKPSIAIWGLLYLTFLAFVILSG
                     DTGHSTAH"
     gene            complement(2196..2984)
                     /locus_tag="PLE93_00675"
     CDS             complement(2196..2984)
                     /locus_tag="PLE93_00675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH85589.1"
                     /translation="MSNWDKGSYEVTAEQIAPATPAVADAVEPVTGKLALDLACGTGN
                     EALELARRGARVTGYDGAPRLLQVAGERAAAEGLELERVQGDLAELPFDDDSFEIVTS
                     VFGLIFTGSPQDSAAEIGRVLRPQGRIAFTSWVEEGLFQYMQDMAKAAVASHFGQAPP
                     EGADAPFAWGDEVAVRELFSESGLMLQVETRELVIEEESAAALNDRWFDLHPIWITMQ
                     EVIGDEAYEEMRREALPVVESHNEASDGSFRNTLRYLLFEGSPV"
     gene            complement(2986..3456)
                     /gene="bcp"
                     /locus_tag="PLE93_00680"
     CDS             complement(2986..3456)
                     /gene="bcp"
                     /locus_tag="PLE93_00680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNH85590.1"
                     /translation="MLEEGDKAPDFTLEDQLGEKVKLSDHRKELVVLYFYPKASTSGC
                     TTQACSIRDNSADYAKAGATVIGISPDEVKDISRFATKNDLEFTLVADKDHKVAEKYG
                     TWVEKSMYGNKYMGVQRATFIIRGGKILRVFPKVQPKRHDGLVLGALAEINEEN"
     gene            complement(3497..4381)
                     /locus_tag="PLE93_00685"
     CDS             complement(3497..4381)
                     /locus_tag="PLE93_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNH85591.1"
                     /translation="MADRGPLTLLGVPIDSVGTAGGTELGPNVLRQLLGPEGFEDAGD
                     TSRKIRGLERDRANGWLAFDDILAMTGEVRGRVADLTAAGKVPVLLGGCCTLLPGALA
                     GARDSLGEFGLAYFDGHLDLFTGLTSPTGEGADFPVAASLGMAPDPLLEVIGETPVVA
                     PGRMSLVGARDKEELQLISPLPEGLGIGRIEFRDDLRHADLNEVGASLEAELTDGGSG
                     FWLHLDVDVLDREAFPATDYLMPDGLSMDELRALFAPLASSPGLIGVDVTCFNPEKDP
                     DERCGTELAELLRGTLNG"
     gene            complement(4381..4713)
                     /locus_tag="PLE93_00690"
     CDS             complement(4381..4713)
                     /locus_tag="PLE93_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85592.1"
                     /translation="MDDSTSRYELEELVVQPGTYFNPQTEVVVVVDDSASIDQEIFNM
                     ESYEGADWVRIADDVAVDEQQRDELLEHFQTHYHPGNGGSVSGTALEQGDDELDEDEE
                     GQEIGRED"
     gene            complement(4743..5171)
                     /locus_tag="PLE93_00695"
     CDS             complement(4743..5171)
                     /locus_tag="PLE93_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85593.1"
                     /translation="MVAELSFVLILLCVVVVPVAAIAFARSGKAYEEIGKGRFAVDFD
                     DSSETDHHEELRQLVEAKAYRQARRGEQPVDVEREVERLSKGEPEETADAAPPPDPAT
                     AGIRAEIREVVIAKNESRERRGEEPLDVESEVERMLAEFG"
     gene            5312..>6049
                     /locus_tag="PLE93_00700"
     CDS             5312..>6049
                     /locus_tag="PLE93_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH85594.1"
                     /translation="MVDFTLSDEQKDLREMAHNFAEKEMRSVAFEYDKDGTWPAEVIQ
                     KAWEVGLMNTHVDEAYGGPGLDYLSGCLIEEEFGWGCSGIGTSLACNGLASAPVALGG
                     SEETKAKYLGMLCEEPKLASFCLTEPDAGSDVAGMKTRAVKKGDKYVINGSKCFITNG
                     AHADWFTVYAKTDPDAGSRGITAFVVDKQDGITVDKKEDKMGQRASNTVTLTFNDVEV
                     PAENMLGEENKGFKLAMMTLDRTRPGVA"
BASE COUNT         1037 a         2011 c         1890 g         1111 t
ORIGIN      
        1 ccgcacctgg tcgaccccct tgctctgcag cagcccgctc atccaggcgg cgacccggtc
       61 ccagttgcgg cgcatctcgg cttcggcgag cacctcctgg ccccaaagcc cgggcgaaac
      121 ctcgaccgga tcagggccga gttcgacccc gagggaatcg gagaggctgt gggcggggtc
      181 ggtcgagagg atcaccgtgc gtctgccttc ggctgcgcag cgacgggcgg tggcggcggc
      241 aacgctggtc ttgccaacgc ctcctttgcc tgtgtagagg atggtgcggg gtggcattca
      301 agggcgagcg tatagcctgc cccgacttgg aacggacgcg gatccccctt gttctggtgc
      361 tcgtgctggc gttctcgctg gtgatgcagg cctgcggcag cagtgacccg gacccggcga
      421 cgattctgga ccgggccctg accagggagc atctttcgag cttcggcaac ggatcgggcg
      481 gtcccaccgg gggcatcgtt tcggtcgagg cacttggcta cgaggaccgg gttctggacg
      541 agcggaaggt ggcggccgac ccgcaggtga tgtctgacat ccgcagcgcc ctgggctccg
      601 attcggggct gcgcggactg gtcggcgacc ttcggtacga cgggaccgaa gaggtttccg
      661 gggtcgggac cgaccacatc tccggtgaac tcgacgtggc cggcctggcc agggcactgg
      721 aagcggccgg gggcggggag gtgggccaga tcgccggggt caaaaccgga tccagccttg
      781 aggattcact cgtttcagcc gatttcgacc tgtacgccgg ccagcaggat ggcgacatcc
      841 gccggctcga tctgaccctg gcccttgacg acccggacaa tgcacttccg cctgcccgga
      901 tacggttcag cctgacgccg cgaccatccg gcgcaaacca gacctagagg acctccccct
      961 tacccatgga atacctgctc ctgcttttca gcttcgcgat catcctgacc ggcgcgctgc
     1021 tcttcaccaa cgcgatcgag tggctcggtc acaagctcaa cctgggtgag ggcgcggtcg
     1081 gttcgatcct ggcggcagtg ggcaccgcga tgccggaaac cctgatcccg atcgtcgcga
     1141 tcatcggcgg ggtcagtggc tccgacgagg ttgcggtcgg ggcgatcgtc ggggcgccgt
     1201 tcctgctggc gacgatcgcg atggccctgg tcggcatctc ggcgctcgcc tacatcaaac
     1261 gccggccgca gggcaagcac ctcgacgtcc acgtcgaaac cctcgaccgg gacctggtct
     1321 tcttcatgat cttcttcacc ctggccctgg tggtcggggt ggttgacgtt ccacagtcgg
     1381 tgcagatcgg cggcggagtg atctgcctcc tggcttacgt ctactacgtg gtgcagacga
     1441 tccgcaacag cggtgacgtt gccgagagcc acgagatgga tcccctgatc atggatcgcc
     1501 ggaccgaccg caccggcggg ccgcccaact cgatcatgat catccagctg ctggtcggtc
     1561 tcgcctcaat ggtcggcggt gcccatctct tcgtccacga actgctgagc gtggccgagc
     1621 atctcggcgt ttcggcgatc atcctctcgc tgatcctcgc gccgctggcg accgagctgc
     1681 cggagaaggc gaactcgttc ttctgggtcc gcgacggcaa ggatgccctc gccctcggca
     1741 acatcaccgg agcaatggtc tttcagtcga ccatccccgt cgcgatcggc atgatcttca
     1801 tcgactggtc gctggacaag tacgcggtcc tctcgatgct cctcggcctc gccggcggtg
     1861 tcgtcgccta ctggtcactc cgccggaacg gccagttcaa caagccttcg atcgcgatct
     1921 ggggtctgct ctacctgacc ttcctcgcct tcgtgatcct cagcggcgac accggccact
     1981 cgaccgcgca ttagccggaa aaacccaggc gagacgcaac gtcccgtctc gccgcaggac
     2041 cacaaaacgt caaatccaga cgagacgcaa cgtcccgtct cgccgaagga ccacaaaacg
     2101 tcaaatccag acgagacgca acgtctcgtc tcgccgcagg ttcacaaaac gtcacatcca
     2161 ggcgagacgc aacgtctcgt ctcgcgggag ttttttcaga cggggctgcc ctcgaagagg
     2221 aggtaacgca gcgtgttgcg gaagcttccg tcgctggctt cgttgtggga ttcgaccacc
     2281 ggcaacgcct cgcggcgcat ctcttcgtag gcctcgtcgc cgatcacttc ctgcatggtg
     2341 atccagatcg ggtgaaggtc gaaccagcgg tcgttcagcg ctgcggccga ttcctcttcg
     2401 atcacgagtt ccctggtttc gacctgcagc atgaggccgc tttcggagaa cagttcacgc
     2461 acggcgacct cgtcgcccca cgcgaacgga gcgtcggcgc cttccggcgg cgcctggcca
     2521 aagtgggagg caactgccgc cttcgccatg tcctgcatgt actggaagag accttcctcg
     2581 acccatgaag tgaaggcgat ccggccctgg ggccgaagga cccggccgat ctcggcggcg
     2641 gaatcctgcg gcgagccggt gaagatgagc ccgaagacag aggtgacgat ctcgaagctg
     2701 tcgtcgtcga agggaagctc cgcgaggtcg ccctgaaccc gttccagctc gagtccctcg
     2761 gctgcggctc tctcgcccgc cacctggagc aggcgcgggg caccgtcata tccggtcacc
     2821 ctggcgccgc ggcgggccag ctcgagcgct tcgtttccgg tgccgcaggc gaggtcgagg
     2881 gcgagcttgc cagtgaccgg ttcgacggca tcggcgaccg cgggagtggc gggcgcgatc
     2941 tgttcggcag tgacttcgta tgagcccttg tcccaattgg acatctcagt tctcctcgtt
     3001 gatctcggcc agtgccccca ggacgaggcc gtcgtgcctc ttcggctgga ccttggggaa
     3061 gacccgcagg atctttccgc cccggatgat gaaggttgcc cgctgcacgc ccatgtactt
     3121 gttcccgtac atcgactttt cgacccaggt gccatatttc tccgcgacct tgtggtcctt
     3181 gtcggcgacc aaggtgaact cgaggtcgtt cttggtcgcg aaccttgaga tgtccttgac
     3241 ctcgtccggc gagatcccga tcaccgtcgc gcccgccttc gcgtaatcgg cactgttgtc
     3301 gcggatcgag caggcctggg tggtacaccc gctggtactg gccttcggat agaagtagag
     3361 gacgaccagt tccttgcggt gatcggagag ctttaccttc tccccgagct ggtcctcaag
     3421 tgtgaaatcg ggagctttgt cgccttcttc gagcatggcg ggatcttatg gggcactcat
     3481 ggcgcgagcc gccgggtcat ccattcaggg ttccgcgcag cagttcggcc agctccgtcc
     3541 cgcatctctc gtcgggatcc ttttccgggt tgaagcaggt gacgtcaacc ccgatcaggc
     3601 ccggagagga agccaggggc gcgaacaggg cgcggagctc atccatgctc aacccgtcgg
     3661 gcatcaggta gtccgtcgcg gggaaggctt cacggtcgag gacgtcgacg tcgaggtgaa
     3721 gccagaatcc ggagccacca tcagtcagtt cggcctcgag cgaggcgccg acttcgttca
     3781 ggtcggcgtg cctgagatcg tcgcggaact cgatccggcc gatgccgaga ccctccggca
     3841 gcggcgagat caactggagc tcctccttgt ctcttgcgcc gaccagagac atccgcccgg
     3901 gcgcgaccac cggtgtctcg ccaatcacct cgagcagggg atcgggagcc attccgagcg
     3961 atgcggcgac cggaaagtcg gccccttcac cggtcggcga agtcagcccg gtgaaaaggt
     4021 cgagatggcc gtcaaagtag gcgagaccga attcgccgag cgaatcccgg gctccggcca
     4081 gcgcgcccgg caaaagggtg cagcagccgc cgaggagcac cggcaccttc ccggcggcgg
     4141 tcaaatccgc gacccggccc ctcacctcgc cggtcatcgc caggatgtcg tcgaaagcca
     4201 gccagccgtt ggccctgtcc cgctccagtc cgcggatctt gcggctggtg tcgccggcgt
     4261 cctcgaaccc ttcgggcccc agcagttgcc gcaagacgtt cggtccgagc tcggtacctc
     4321 cggccgtgcc cacgctgtcg atcgggacgc ccaggagggt cagcggcccg cgatcggcca
     4381 tcagtcttcg cggccgattt cctggccttc ttcgtcctcg tcgagctcgt cgtcaccctg
     4441 ttccagggcg gtgccggaaa ccgacccgcc gtttccgggg tggtagtgag tctggaaatg
     4501 ctcgagcagt tcatcgcgtt gctgctcgtc gaccgcgaca tcgtcggcga tcctcaccca
     4561 gtcggcgccc tcgtaggact ccatgttgaa gatctcctgg tcgatcgacg ctgaatcgtc
     4621 aaccacgaca acgacttcgg tctgcgggtt gaagtaggtc cccggttgaa ccaccagctc
     4681 ttcaagttcg taacgggaag tcgagtcatc catgagccaa tactacccac gacccgtcgc
     4741 cgctagccga actcggccag catccgctcg acttcggatt cgacgtcaag cggttcctcg
     4801 ccccggcgct cgcggctctc gtttttcgca atgaccacct cgcggatttc cgcgcgaatt
     4861 cctgcggtcg cgggatccgg cggcggagcg gcatcggccg tttcttccgg ttctcctttc
     4921 gagagccgct cgacttcccg ctcgacgtca accggttgct cgccccgtct cgcctggcgg
     4981 taggccttgg cctcgaccag ctgtcggagc tcctcgtgat ggtcggtctc gctgctgtca
     5041 tcgaagtcga ctgcgaatcg tcccttcccg atttcttcgt acgccttgcc cgagcgggcg
     5101 aaggcgatcg ccgcgaccgg caccacgacc acgcaaagga ggatcaggac gaaagaaagt
     5161 tccgcaacca tccccccaag agtaaaggcg cttggtaccc tgttcgaaga gttattgatt
     5221 gacaagtcaa tcgggagccg cccgggcctc ctcgccccgg ccccgcatcg agcaaatcga
     5281 acggttccaa ccgacctaag gagcttgaag agtggttgat ttcacgctga gcgacgagca
     5341 gaaggatctg cgcgaaatgg cgcataactt cgccgagaag gagatgcgaa gcgtcgcctt
     5401 cgaatacgac aaggacggca cctggcctgc cgaagtgatt cagaaggcct gggaagtcgg
     5461 cctcatgaac acccatgtag acgaggctta cggcggcccg ggactggatt acctttccgg
     5521 ctgcctgatc gaggaagagt tcggctgggg ctgctccggc atcggcacct cgctcgcctg
     5581 caacggcctc gcttcggccc cggtcgccct cggcggctcc gaagagacca aggccaagta
     5641 cctcggcatg ctctgtgagg agccgaagct cgcctccttc tgcctgaccg agccggacgc
     5701 cggctccgat gtcgccggca tgaagacccg cgccgtgaag aagggtgaca agtacgtcat
     5761 caacggttcc aagtgcttca tcaccaacgg tgcccacgcc gactggttca cggtctacgc
     5821 caagaccgat ccggacgccg gcagccgcgg catcaccgcc ttcgtggtcg acaagcagga
     5881 cggcatcacc gtcgacaaga aggaagacaa gatgggtcag cgggcctcga acaccgtgac
     5941 cctcaccttc aacgacgttg aagtcccggc cgagaacatg ctcggcgaag agaacaaggg
     6001 cttcaagctc gcgatgatga ccctcgaccg cacccgtccc ggtgtggcc
//
LOCUS       DAOQFI010000019         5731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_5463_length_5731_cov_3.195208, whole genome shotgun sequence.
ACCESSION   DAOQFI010000019 DAOQFI010000000
VERSION     DAOQFI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5463_length_5731_cov_3.195208"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLE93_00705"
     CDS             <1..341
                     /locus_tag="PLE93_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85595.1"
                     /translation="FLTTIFALTQLGIIEDFFPGWVYFLAASQLFFGNFVFVYLGLAA
                     SVRRGDYSLAPYALFLPFYWGLMSVAAWKGFGQLVTNPFYWEKTEHGLDLGGGAEHAH
                     TTTPRKAGTV"
     gene            341..2287
                     /locus_tag="PLE93_00710"
     CDS             341..2287
                     /locus_tag="PLE93_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85596.1"
                     /translation="MADERIDINPVIDDGGTAAVQEEVRTRRVRRTYVDPEVASSIGE
                     VPPEPRPFSPDAPRANWESGIVFAVFAIIYGVLDYFMLTDGRIVDFISLAHLNEAYMV
                     FWNDPPRLAAIGLDAATFSSIAYMPLSLVKPLATSLVAMPVLGAITAGLLMASLNSLM
                     RICEINLIFRIVLLILFGLNPMFAFFAANGGPETLGLCLLAIALSSTIAWSVTDQTRH
                     IAGAGLAMGLAVMVDYGYILVGLGFMVAIMIISASKNDDGLKTRSSLLLFLTPVAYAL
                     LFWCLMNWVLLGDPFQWVTAQNGVIQVNTTGALQAITTDVGGSLGDLFEVTLGVAPLA
                     LVAGFLLIIGSFLKKDATGWGLLVVGLCTAAPVIRAVAADQADLLTISYGLPLAVFAV
                     GAVAWFGKDGWGVVAAVILGVGLIAAIPLSWNTMRDYRFQNQAEAFTRWVEHRDSQEG
                     TKSLGDYTVGIDPELSMARFINNKIPQEKRSILVDQNFSYGVMITSGRPQNFLDRVDA
                     GEDDWQATIDNPWGKDGERDVSYMLITTSREGDQIAKKWPTAVQGGEAGFTPIFRTDR
                     YVLVKVSETKPPSDESDQQSNEPRTTPNPVTPTSPLNPNGPDLTTPSQSETDVTPVSP
                     SPSNNGSTVEPNNGTSSAPSLEGE"
     gene            2287..3570
                     /locus_tag="PLE93_00715"
     CDS             2287..3570
                     /locus_tag="PLE93_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85597.1"
                     /translation="MPNRSMQRWILVIALTALLGLLVAACGSNDSDNGDSGDNSAKST
                     TGGTGATGDTAATANETTGATEPGTGSAVTAGTDPVAEGRLFSADSPWNTTAEGLPID
                     DASDRMLELAQFRRAAKEVPGQEGVQTFTRKIVDDGIFVNSQVWAPLIVAANTQDSVN
                     TKFVCRQSKCSSADPKVPSTLNIPANTTPDPRYDGWLSVIDRENGMGYDFWRARREGD
                     GTISYQFGKAWTLDGPGFSKPVSIDPDRAVGARGSGLPLFAGVIGPQELERGEISHAL
                     AISVPGLARRNFVQPASVTDGNGDRKSLPAGARIRLRADVSINEKKLRTLKGQRRRSA
                     DAILTALRTYGAIVVDRAAVPSLYAPLGTSPKLLRGDELDWLKLDDFEVISLPQIYKD
                     PPLSQVGLEGPETGGDNSQVQSGSAASGNEVGGGL"
     gene            3576..4997
                     /locus_tag="PLE93_00720"
     CDS             3576..4997
                     /locus_tag="PLE93_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNH85598.1"
                     /translation="MLKNKRAGIACASAVLMLLVAGCGVNPDEAPESAGHDGSDANFV
                     PAQTGNSKTKEAKPTPREVGTIRIAGKAQGSLTPDLVRTYNAAGGTANVTVDEEGENQ
                     AFQQFCAGKADVVDSARPISEAEYRKCIANGIQPVQFQVASDAVVLAIKNETDVGTDC
                     LSFDEVRNIFRAGSDINSWSQVGYGHDLGGDTNEPRMKVAGPDEDSNVFGFFGQYVLG
                     APEPTMLDFRSDYQAFPTDAGVRRAVVGSNRDFRAAAQFQRSRQVYQGILDSLAENRR
                     AVRDARAEVAKGIADERPPEDQARDRRKLAKAQANLENLEKSLPAARRYARQNRAALR
                     RLAEVRGTLGLFRFSYYEAFEEQLRPMEVSASNDFAQPECIFPSQATVTNATYPLARQ
                     LLLTINYKNMTDQDINAFLVQSLSRSQQLAEDAALVPLPDETQATELAWLNGTSEPDI
                     IFYESGAGTSVGTQQEADSASGE"
     gene            5141..>5731
                     /locus_tag="PLE93_00725"
     CDS             5141..>5731
                     /locus_tag="PLE93_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85599.1"
                     /translation="MPAASGAAAKKKRVGPCSTLPINTKLSTHDPTGLLEDGYAWLYL
                     NPKGTVRSPNVKVVRHGRVFARGRIVGSLVPGRTTLVKMHVGRRMQQGKYRIRVTASK
                     PGRCSKRITKTRVWKFHRPTLGLKAIPVSTRIGDNVGVVRFALRPVRRTKVGWTRVSL
                     IGPGGGVVSQQVIKSIGGKQILAELPIKGKLKPAKYR"
BASE COUNT         1061 a         1854 c         1769 g         1047 t
ORIGIN      
        1 ggttcctgac caccatcttc gcgctgaccc agctcggcat catcgaggac ttcttccccg
       61 gctgggtgta cttcctcgcc gcgtcacagc tcttctttgg caacttcgtc ttcgtttacc
      121 tggggctcgc ggcttcggtc cggcgtggtg attacagcct cgcgccctac gcgctcttcc
      181 tgccgttcta ctggggcctg atgtctgtcg cggcctggaa gggcttcggt cagctcgtaa
      241 ccaacccgtt ctactgggag aagactgaac acggactcga cctcggcggc ggtgccgaac
      301 atgcccacac gacgactccg cggaaagcgg gcaccgtctg atggccgacg agcggatcga
      361 cattaatccc gtaattgacg acgggggtac cgccgcggtt caggaagaag tcaggaccag
      421 aagggtccgt cgcacctatg tcgaccctga ggtcgcgtca agcatcggtg aagtcccgcc
      481 cgaaccgcgg cctttcagcc cggacgcgcc cagggccaac tgggagagcg gcatcgtctt
      541 cgcggtcttc gcgatcatct acggcgtcct cgactacttc atgcttaccg acgggcgcat
      601 cgtcgatttc atttcgctgg cgcacctcaa cgaggcctac atggtcttct ggaacgaccc
      661 tccgcgcctg gcggctatcg gcctggatgc cgcgaccttc agttcgatcg cctacatgcc
      721 gctcagtctg gtcaagccgc tggcgacctc gctggtcgcg atgccggttc tgggggcaat
      781 tacggccggt ttgctgatgg catctttgaa ctcgctgatg cggatttgcg agatcaacct
      841 gatcttccgc atcgttctgc tgatcctgtt cggcctgaat ccgatgttcg cgttcttcgc
      901 cgccaacggc ggcccggaga ccctcggtct gtgcctgctc gccatcgcac tctcatcgac
      961 gatcgcctgg tcggtcactg accagacccg tcacatcgct ggtgccggtc tggccatggg
     1021 gctggccgta atggtcgatt acgggtacat cctcgtcgga ctgggcttca tggtggcgat
     1081 catgatcatc agcgccagca agaacgatga cggactgaag acccgctcca gcctgctgct
     1141 cttcctgacc ccggtcgcct acgcgttgct cttctggtgc ctgatgaact gggtgctcct
     1201 gggtgacccg ttccagtggg tgaccgccca gaacggggtc atccaggtca acaccacggg
     1261 ggccctgcag gcgatcacca ccgatgtagg gggcagtctc ggagacctgt ttgaagttac
     1321 ccttggggtg gcgcctttgg ctctggtagc gggattcctg ctgataatcg gctcgttcct
     1381 gaagaaggac gcgaccggct ggggcctgct ggtagtcggg ctctgcactg ccgcgccggt
     1441 gatccgggcc gtcgccgccg accaggcgga tctgctcacc atttcctacg gtctgccgct
     1501 tgcggtgttc gcggttggtg cggtggcctg gttcggcaag gatggctggg gggtcgtcgc
     1561 tgcagtaatc ctcggggtcg ggctgatcgc ggcgataccg ctgagctgga acacgatgcg
     1621 ggattaccgg ttccagaacc aggcggaggc atttacccgc tgggtcgagc atcgcgacag
     1681 ccaggagggc accaagtctc tgggcgacta cacggtcggc atcgatccgg aactctccat
     1741 ggccagattc atcaacaaca agattcccca ggagaaacga tcgatcctcg ttgatcagaa
     1801 cttcagctac ggcgtgatga tcacctccgg gcgtccccag aacttcctcg accgggtcga
     1861 cgcgggtgaa gatgactggc aggcgacgat tgacaacccc tggggcaagg acggcgagcg
     1921 ggacgtcagt tacatgctga tcaccaccag ccgcgaaggc gatcagatcg ccaagaagtg
     1981 gcctaccgcc gtccagggcg gagaagcggg gttcaccccg atattccgaa ctgaccgcta
     2041 cgtgctggtg aaagtctccg agaccaagcc gccttcggat gagagcgatc agcagtcgaa
     2101 tgagccgcga acgactccga atccggtcac cccgacttcg cccctgaatc cgaacggtcc
     2161 ggatctgacc acaccgtccc agtctgaaac ggatgtgact ccggtttcgc catcgccgtc
     2221 caataacggc agcacggttg aaccgaacaa cggtacgtcc tccgccccga gcctggaggg
     2281 tgagtagtgc cgaatcgcag catgcagcgc tggatcctcg tgatcgcgtt gacggccctg
     2341 ctcggcctgc tggtcgctgc ctgcggttcg aatgattcgg acaatggtga ttccggcgac
     2401 aactcggcca agtcgactac cggcggcacc ggagcaactg gcgataccgc tgcgaccgcg
     2461 aacgaaacga ctggtgcaac cgagcccggc accggttccg cggtgaccgc tggcaccgat
     2521 ccggtcgccg agggccgcct tttctcggcc gacagtccct ggaacacgac ggccgagggt
     2581 ctgccgattg atgatgcctc cgaccggatg ctggaactag cccaattccg aagggctgcg
     2641 aaggaggttc ccggccagga aggcgttcag accttcactc gaaagatcgt cgacgacggc
     2701 atcttcgtca actcgcaggt ctgggctccc ctgatcgtcg cagccaacac ccaggattcg
     2761 gtcaacacca agttcgtctg ccgtcagtcg aagtgcagct cggctgatcc caaggttccg
     2821 agcacgctga acatccccgc gaacaccact cccgacccgc gctacgacgg ctggctcagc
     2881 gtgatcgacc gggaaaacgg tatgggatat gacttctggc gtgcccgccg tgaaggcgac
     2941 ggcaccattt cctaccagtt cggaaaggcc tggaccctgg atggccccgg cttcagcaag
     3001 ccggtcagca tcgacccgga ccgggcggtt ggtgcgcgtg gatccgggct gccgctcttc
     3061 gcgggtgtta tcggccccca ggaactggag cggggcgaga tcagccatgc cctggcgatt
     3121 tcagttcccg gcctcgcccg tcgcaatttt gttcagcccg cttcggttac tgacggcaac
     3181 ggcgatcgca agtcgctgcc ggccggggcc cggatccgtt tgcgcgcaga cgtgtcgatc
     3241 aacgagaaga agctccgcac cctgaagggg cagcgtcgac gctcggccga cgcgatcctg
     3301 accgcgctca ggacctacgg cgcaatcgtg gttgatcggg ccgcggtgcc cagcctctac
     3361 gccccgctcg gtacctcgcc gaagctgctc cggggtgatg aactcgactg gctgaagctg
     3421 gacgattttg aagtgatttc gctgcctcag atctacaagg atccgccact aagccaggtc
     3481 ggcctcgaag gtcccgagac cggcggtgac aattcgcagg ttcagtccgg cagcgccgcg
     3541 tctggcaacg aagtcggagg tggactgtga gtcgggtgct gaaaaacaag agggccggta
     3601 tcgcctgcgc atcagccgtg ctgatgctgc ttgtcgccgg ctgtggcgtg aatccggatg
     3661 aagcacctga gtcggcgggt cacgacggct ccgacgcgaa cttcgtgccg gcccagaccg
     3721 gcaacagcaa gaccaaggaa gccaaaccca ccccgcgtga ggtcgggacc atccggatag
     3781 ccggcaaggc ccagggttcc ctgacccccg atctggtcag gacctacaac gcggctggtg
     3841 gcaccgcaaa cgtgaccgtc gacgaagaag gcgagaacca ggccttccag cagttctgcg
     3901 ccggcaaggc cgatgtcgtc gattcggccc ggccaatctc cgaagccgaa taccgcaaat
     3961 gcatcgccaa cggcattcag ccggtccagt tccaggtcgc ctcggacgcg gtcgtccttg
     4021 cgatcaagaa cgagaccgat gtcggcaccg actgcctcag cttcgatgaa gtccggaaca
     4081 tcttccgggc cggttccgac atcaacagct ggagccaggt cggttacggc cacgatctcg
     4141 gcggcgacac gaacgaaccg aggatgaagg tcgccggacc ggacgaggat tcaaacgtct
     4201 tcggcttctt cggccagtac gtgctcgggg ctccagaacc gaccatgctc gatttccgca
     4261 gcgactacca ggccttcccg accgacgccg gggtgcggcg tgcggtcgta ggcagcaacc
     4321 gggacttccg ggcggccgcc cagttccagc gctcgcggca ggtctaccag gggatcctcg
     4381 attcgctggc cgagaaccgc cgcgcggtgc gtgacgcccg ggccgaagtc gccaagggaa
     4441 tcgccgacga acgcccgcca gaggatcagg cccgcgatcg ccgcaagctg gccaaggccc
     4501 aggcaaatct cgagaacctc gagaagtccc tgcctgcagc ccgccgctat gcgaggcaga
     4561 atcgcgccgc cctccgtcgc ctggcggaag tccgcggaac gctcgggctc ttccgcttct
     4621 cctactacga ggcattcgaa gagcagctgc ggccgatgga agtcagcgca tcgaacgatt
     4681 tcgcccagcc ggaatgcatc ttcccctcgc aggctacggt taccaacgcg acctacccgc
     4741 tggcccggca actgctcctg accatcaatt acaagaacat gactgaccag gacatcaacg
     4801 ccttcctggt ccagagtctc tctcgttccc agcagcttgc tgaagacgca gccctcgtgc
     4861 cgctgccgga tgagactcag gcgaccgagc ttgcctggct gaacggcacc tccgagccgg
     4921 acataatttt ctacgaatct ggcgcaggaa cctcggtcgg aacgcagcaa gaggccgatt
     4981 cggccagtgg cgagtagccg caattcctca cagatccacg cggattttcc gctactcggc
     5041 cttgaacgcg ggtttaaggc ggggtgagat catgattaac gtgtttcggc gtatagcgac
     5101 aatcctcttg ctcatcggtc tgggttttac cttggccgcg atgcctgctg cttccggcgc
     5161 ggccgcgaaa aagaagcggg tcggtccctg ctcgaccttg ccgatcaaca ccaagctgag
     5221 tacccatgac ccgaccgggc tactggagga cggttatgcc tggctgtacc tgaaccccaa
     5281 gggcaccgtc cgctcgccga acgtcaaggt tgtacggcat ggccgcgtgt ttgccagagg
     5341 ccggatcgtc ggcagtctcg taccgggtcg caccacgctg gtcaaaatgc atgttggccg
     5401 tcgcatgcag cagggcaagt atcggatcag ggtgaccgcc agcaagccgg ggcgctgcag
     5461 caagcgaatc accaagaccc gcgtctggaa atttcaccgg cctaccctcg ggctgaaggc
     5521 gattccggtc agtacccgga tcggtgacaa cgtcggcgtc gtccgcttcg ccctgcgacc
     5581 ggtccgccga accaaggtcg gctggaccag ggtttcgctg atcggtcccg gcggtggcgt
     5641 tgtcagccag caggtgatca agtcgatcgg cggcaagcag atcctggccg agctgccgat
     5701 caaaggcaag ctgaagccgg ccaagtaccg g
//
LOCUS       DAOQFI010000020         5573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_5721_length_5573_cov_3.488583, whole genome shotgun sequence.
ACCESSION   DAOQFI010000020 DAOQFI010000000
VERSION     DAOQFI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5721_length_5573_cov_3.488583"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLE93_00730"
     CDS             <1..243
                     /locus_tag="PLE93_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85600.1"
                     /translation="SILAALLAGMRKVMPGLGAPPRSYDPDRGPPAARAAILAAFVAV
                     LVHTLTYAGFLDDPVTWVLIAIGYSLAFPCRATSAA"
     gene            219..1043
                     /locus_tag="PLE93_00735"
     CDS             219..1043
                     /locus_tag="PLE93_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNH85601.1"
                     /translation="MSGYLRRLATTGAAYTAASIISKLIAVALLPLYTRYLTPADYGA
                     AEVMFSAVVAASIIIRLGVVEALLRFYYKQGEEPAAVVSTSFATLFWAGLISVIVLMP
                     FAGPISQLLLGESEPTLGRIAIVGLFVLTLSEYLLTIFRLDERARAFFTVTMLSVLIT
                     IPVTVILVVPLDLGAEGLLAGSYGTGLAIVLGLIFYNRRRLALAPDRPLLKRMMAFGL
                     PTMPAELSLYSLSFIDRILIARKLGLAEAGLYSIAIKMSQGIAVLVLPAARLLDPG"
     gene            1072..1785
                     /locus_tag="PLE93_00740"
     CDS             1072..1785
                     /locus_tag="PLE93_00740"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNH85602.1"
                     /translation="MVTWFVTVMAFVVSFLWLIAPWIVGLLAAPEFSEAAVVVGPVSA
                     GAALYALYMVLLVVLGRTGRTEYNLPATIAGLVVNVGLNLLLLPLWGIIGAGVALVVS
                     YLVVVVLMYLFTQRLFPVPYEWLRLVRVLLATAALVAIGEVFVPDDGLAWLAVRLLLV
                     LALPFALLLTGFFTAEERGWLLLLTKPGELKAKILAAREVGKAAGEEGEGTSASGRPG
                     SRGLPEAVEVEQWDEDLRS"
     gene            complement(1843..2808)
                     /locus_tag="PLE93_00745"
     CDS             complement(1843..2808)
                     /locus_tag="PLE93_00745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH85603.1"
                     /translation="MNSPSPAPDPATEATEVDLSYCVVNNDGREYLMDCLAAIEKFHP
                     AGLESEVLVLDNASSDGSVEAVREQFPSAEVIAMESKQGKAANDSLLLERARGRYCLL
                     LNEDSELRAGAAGALVAALDAEPEAGAAGAQLFDGEGNAYACAWRFPGVESAFVGALF
                     LHSRLTVESKGEETRRVDWAQSSALMVRREAAREIGGLDPQFFVYYDECDFCKRLADA
                     GWSTLYVPSAEAVHHNQLSTDLSSGLPRIVEFHRNRDLYMRKHHNRAAAFTVRLLTAW
                     AYGLRTIASVFMPGAPTPVYRAHTRQALFPYRGESLADRASSREP"
     gene            2832..3992
                     /locus_tag="PLE93_00750"
     CDS             2832..3992
                     /locus_tag="PLE93_00750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH85604.1"
                     /translation="MHVKLIDPSAFTPPYDRSLAAALAAEGAEVELITSEFTYGPVPE
                     PEGYEVSLDFYRRISPRARRFSKALQHFPEMRRLRRRLENAPDPMVTHYQWLTMPRFD
                     HRLISKRRPRLMTAHYILPPGPNGKQAAAAKRVFGAMDAVIAHSKGGASRLVEEVGLP
                     EERVRVIPHGAFDYLTRLPEEAPLPPELADSGAEAPVILFFGLLRPYKGIDVLIRAAG
                     QLAADADRDGPAPELWIVGNPRMDLTELVERSRKLKVKVRWLPRFIEDQEIPAIMRRA
                     DVVVLPYLDGEQSGVLYTGIAFGKAMVVSDVGGIGEVAREHGLARTVEPGDVDQLAAE
                     LSALVLGDRAAAAREELETRCRVAAEGPFAWAAVARQTIALYEDLLGRGSGS"
     gene            3989..5167
                     /locus_tag="PLE93_00755"
     CDS             3989..5167
                     /locus_tag="PLE93_00755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH85605.1"
                     /translation="MIVVYILFWLSAGLVFFTHIGYPITLWLLDFIGLGGRAGEVRGR
                     KLSEIPPAIDPDTEEEILPSVSLIIAAYNEETVIEAKVKNALELEYPRELLQVIVASD
                     GSSDRTVEIAREAGADLVLDLPRGGKIAAQNAAAEAATGNLLAFSDANSTWEPGALAE
                     LIDPFRDERVGYVCGQVRFIGPDGGNLEGAYWRYEMRVREMESELAGVTAGNGAIYAV
                     RASAYIPLAPSGSHDLSFPFALAKRRLASLYRPRARATEKLVPSLDGELARKRRMMVG
                     LWDIVVGEGMLDPRGYSPLYLFELFSHRLLRYLSPALHLIAFLTNLALLGHGWFYGLT
                     MLLQLLLVAGAILGRWIPILPFRISRYYLEVTLSIALGLWDRWRQGPPAAWEKAEGTR
                     "
     gene            5199..>5573
                     /locus_tag="PLE93_00760"
     CDS             5199..>5573
                     /locus_tag="PLE93_00760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNH85606.1"
                     /translation="MSRGFEKLVTALVLVITAPLLVVCAILIRLEGGGSAIYRQTRVG
                     LHGEEFEMLKLRTMIPGSDPVGVGTVVGREDPRVTRVGRWLRRTSLDELPNLVNVWRG
                     EMALVGPRPTIPAQVADYTPHQH"
BASE COUNT          773 a         1905 c         1876 g         1019 t
ORIGIN      
        1 agcatccttg ccgcgctgct ggccggaatg cgaaaggtga tgcccggcct cggtgccccg
       61 ccgcgctcgt acgacccgga ccgcggaccc cccgccgcca gggccgccat cctcgccgcc
      121 ttcgtcgcgg tgctggttca cacgctcacg tacgcaggat ttctcgacga cccggttacc
      181 tgggtcctga tcgccatcgg ctatagcctc gcctttccat gtcgggctac ctccgccgcc
      241 tagcgacaac cggcgccgcc tacacggcgg ccagcatcat ctcgaagctg atcgcggtag
      301 ccctgttgcc gctttacacg cgctacctga ccccggctga ttacggcgcc gcggaagtca
      361 tgttctcggc cgtggtcgcg gcctcgatca tcattcggct cggcgtggtc gaggccctgc
      421 tccgcttcta ttacaagcag ggtgaggagc cggccgccgt cgtctcgact tccttcgcca
      481 ccctgttctg ggcggggctg atctcggtca tcgtcctgat gcctttcgcc gggccgatct
      541 ctcagctcct cctcggggaa tccgaaccga ccctgggccg gatcgcgatc gtcggcctct
      601 tcgtgctcac cctttccgaa taccttctga ccatcttccg gctggacgag cgggcccggg
      661 ccttcttcac ggtgaccatg ctctcggtcc tgatcacgat cccggtcacc gtgatcctgg
      721 tcgtcccgct cgacctcggg gccgaaggcc tcctggccgg cagctacggc accggcctcg
      781 cgatcgtcct cggcctgatc ttctacaacc gccgtcggct cgccctggcc cctgaccgtc
      841 cgctgctcaa gcggatgatg gccttcggcc tgccgaccat gccggccgag ctctcgctct
      901 actcgctgag cttcatcgac cgcatcctga tcgcccgcaa gctcggtctg gccgaggccg
      961 ggctttactc gatcgcgatc aagatgtcac aggggatcgc ggtcctggtc cttcccgccg
     1021 ctcgcctact cgatccagga tgacgacgag gcccgcctcg cctactcggc ggtggtcacc
     1081 tggttcgtca cggtgatggc cttcgtggtc agcttcctct ggctgatcgc tccctggatc
     1141 gtcggcctgc tcgccgcccc cgagttctcc gaggcggccg tcgtggtcgg accggtatcg
     1201 gccggcgccg ccctctacgc gctctacatg gtgctgctgg tcgtgctcgg ccggaccggg
     1261 cgcaccgagt acaacctgcc ggcgacgatc gccgggctgg tcgtcaatgt cggcctcaac
     1321 ctgctgctgc ttccgctctg ggggatcatc ggggccgggg tcgcgctggt cgtctcctac
     1381 ctggtcgtgg tggtgctgat gtacctcttc acccagcgtc tcttcccggt cccctacgag
     1441 tggctgcggc ttgtccgggt gctgctcgcc accgccgccc tggtcgcgat cggcgaggtc
     1501 ttcgtcccgg atgacggtct cgcctggctg gcggtgcggc tgctgcttgt tctcgcgctc
     1561 ccgttcgctc tgctcctcac gggctttttc accgcggagg agcgggggtg gttgctcttg
     1621 ttgacgaagc ccggtgagct gaaggcgaag atcctcgccg cacgggaggt tggaaaagcg
     1681 gcgggcgagg agggtgaagg caccagcgcc tcaggcaggc ccgggagtcg cgggttgccg
     1741 gaggcggttg aggtcgagca gtgggacgag gatcttcgtt cctagaggtg ccgcaggttt
     1801 tgccccttga tggtgctgga tgtgcggcac ctctcccgga actcagggtt cccggctgct
     1861 ggcgcggtcg gcgaggcttt ctccccggta ggggaagagg gcctggcggg tgtgggcgcg
     1921 gtagaccggg gtgggggcgc cgggcatgaa gaccgaggcg atcgtccgca ggccgtaggc
     1981 ccaggcggtc agcaggcgga ccgtgaaggc ggcggcccgg ttgtggtgct ttcgcatgta
     2041 gaggtcgcgg ttgcggtgga actcgacgat gcggggcagg ccggaagaga ggtcggtcga
     2101 gagttggttg tggtggaccg cctcggccga agggacgtag agcgtgctcc agccggcgtc
     2161 ggcgaggcgt ttgcagaaat cgcattcgtc gtagtagacg aagaactgcg ggtcgagccc
     2221 gccgatttcc cgggccgctt cgcggcggac catcagggcc gaggactggg cccagtcgac
     2281 ccgccgggtt tcctcgccct tgctttcgac ggtcaggcgc gagtggagga agagcgcgcc
     2341 gacgaaagct gactcgaccc cgggaaagcg ccaggcgcag gcgtacgcgt tgccttcgcc
     2401 gtcaaacagt tgcgcccccg ccgcccccgc ttccggttcg gcatcgaggg ccgcgaccag
     2461 cgccccggca gcgccggccc tcagctccga atcctcgttc aagagcaggc agtagcggcc
     2521 gcgggcccgc tccagcagca gtgagtcgtt ggccgccttg ccctgcttgc tctccatggc
     2581 gatcacctct gccgagggaa actgttcccg caccgcttcg accgaaccgt cgctggatgc
     2641 gttgtcgagg acgagtacct cgctctccag tccggccggg tggaacttct cgatcgcggc
     2701 caggcaatcc atcaggtact cccggccgtc gttgttgacc acgcagtagc tcaggtccac
     2761 ttctgtggct tcggttgcag ggtcgggtgc cggagagggg ctgttcacgg ttgccaacga
     2821 taatcccggt tgtgcatgtc aagctgatcg acccctcggc tttcaccccg ccgtacgacc
     2881 ggtcgctggc tgctgccctg gcggcggaag gcgccgaggt cgagctgatc acctccgaat
     2941 tcacctacgg gccggtgccg gaaccggagg gttacgaggt cagcctcgac ttctaccgcc
     3001 gcatttcgcc gcgggcccga cggttcagca aggcgcttca gcacttcccg gagatgcgcc
     3061 gcctccggcg caggctggag aacgccccgg acccgatggt cacccattac cagtggctga
     3121 ccatgccgcg gttcgatcat cgactgatct cgaagcgccg tccccgtttg atgaccgccc
     3181 attacatcct gccgcccggc ccgaacggga agcaggccgc ggccgcgaag agggttttcg
     3241 gcgcaatgga cgcggtgatc gcccattcga agggcggggc gagccggctg gtcgaagagg
     3301 tcggcctgcc ggaggagcgg gtgcgggtga tccctcacgg cgccttcgac tacctgaccc
     3361 ggctgccgga ggaagccccg ttgcccccgg aactggccga ctccggtgcc gaggctccgg
     3421 tcatcctctt cttcggcctg cttcgcccct acaagggaat cgacgtgctg atccgcgcgg
     3481 ccggacagct ggccgcggat gccgaccgag acgggcccgc tcctgagctt tggattgtcg
     3541 gcaatccgcg catggacctg accgaactgg tcgaacgctc gaggaagctg aaggtcaagg
     3601 tccgctggct gccccgcttc atcgaggatc aggagatccc ggcgatcatg cgacgggccg
     3661 atgtcgtcgt cctgccatac ctcgatggcg agcagtcggg tgtgttgtac acggggatcg
     3721 ccttcggcaa ggcgatggtc gtctccgacg tgggcggtat cggcgaggtc gcccgggaac
     3781 acgggcttgc ccggaccgtc gagccgggtg atgtcgacca gctggccgcc gagctttcgg
     3841 cgttggtcct cggggaccgg gccgctgcgg ccagggaaga actcgagacg aggtgcaggg
     3901 tcgcggcgga gggacccttc gcctgggccg ccgttgcccg acagacgatc gcgctctacg
     3961 aggacctgct cggccggggg agtggatcgt gatcgtcgtc tacatcctct tctggctcag
     4021 cgcgggcctg gtcttcttca cccacatcgg ctacccgatc acgctctggc tgctcgactt
     4081 catcggcctg ggcggccgcg ccggggaggt gcggggccgc aagctcagcg agattccgcc
     4141 ggcaatcgat cccgacaccg aggaggagat tctgccttcg gtctcgctga tcatcgccgc
     4201 ctacaacgag gagaccgtga tcgaggcgaa ggtgaagaac gcgctggagc ttgagtatcc
     4261 gcgcgagctc cttcaggtca tcgtcgcctc ggacggctca tcggatcgca ccgtggagat
     4321 cgcccgggag gccggcgccg accttgtcct ggatcttccc cggggcggca agatcgccgc
     4381 gcagaatgcc gcggccgaag cggcgaccgg gaacctcctg gccttctcgg atgcgaactc
     4441 gacctgggaa ccgggcgcgc tcgccgaact gatcgacccc ttccgcgacg agcgagtcgg
     4501 atatgtctgt ggtcaggtcc gcttcatcgg acccgacggc ggcaacctcg aaggcgccta
     4561 ctggcgctac gagatgcggg tccgcgagat ggagtcggag ctggccgggg tcaccgccgg
     4621 aaacggcgcg atctacgcgg tccgggccag cgcctacatc ccgctcgccc cctcgggcag
     4681 ccacgacctt tctttcccct tcgccctggc caagcgaagg ctcgcttcgc tttaccggcc
     4741 ccgggcccgg gcaaccgaga agctggtccc cagcctcgac ggcgaactgg cccgcaagcg
     4801 gcggatgatg gtcggcctct gggacatcgt ggtcggggag ggaatgctcg acccgcgggg
     4861 ctactcgccg ctctacctct tcgaactctt ctcgcaccga ctgctccgct acctcagccc
     4921 ggcccttcac ctgatcgcct tcctgaccaa cctcgcgctg ctcggccacg gctggtttta
     4981 cgggctgacc atgctgcttc agctgctgct cgtggccggg gcgattctcg gtcgctggat
     5041 cccgatcctg cctttccgca tcagtcgcta ctacctcgag gtgaccctct cgatcgcgct
     5101 cggcctctgg gaccgctggc gccagggccc gccggccgcc tgggagaaag ccgagggcac
     5161 caggtgagca tgccgggcga caggatggcg gtggctcgtt gagccggggc ttcgaaaaac
     5221 tggtcacggc gctcgtgctg gtgatcaccg ccccgctcct ggtggtctgc gcgatcctga
     5281 tccggctcga gggcggcggc tccgcgatct accggcagac ccgggtcggc ctgcacggtg
     5341 aagagttcga gatgctgaag ctgaggacga tgatccccgg ctcggatccg gtcggggttg
     5401 gcaccgtggt cggccgggag gacccgcggg tgacccgggt cggccgctgg ctgcgccgaa
     5461 cctcgctgga cgagctgccc aacctggtga acgtctggcg gggtgagatg gccctggtcg
     5521 gcccccgccc gacgatcccg gcccaggtcg ccgattacac gccgcatcag cat
//
LOCUS       DAOQFI010000021         5367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6079_length_5367_cov_4.067959, whole genome shotgun sequence.
ACCESSION   DAOQFI010000021 DAOQFI010000000
VERSION     DAOQFI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6079_length_5367_cov_4.067959"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="xseA"
                     /locus_tag="PLE93_00765"
     CDS             complement(<1..898)
                     /gene="xseA"
                     /locus_tag="PLE93_00765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNH85607.1"
                     /translation="MSEELPEPGNPAAAEDAAPTPVGDYARQLKQDLKRLPEATILGE
                     ITNFSQSRVQIYFELRDERGGVPGTMWKREFDALGLPAGTVKVGSQVVVTGAPDYYEG
                     GKNASPSFSFRAIDIRPFGEGDLIARLAALRKQFQAEGLTEPQKNLPRRLLPRRIGVI
                     TAEGGAARQDLMVGLERRGWKGEVVWGFAPVQDRKAAPQITRILSDMAAFADVDSIVV
                     CRGGGSLTDLWAFCDEDLCRTVVMLAVPVISAVGHERDVTLLDDVAAVRASTPTHAAE
                     AVVGIDIPAARASLARAAGTTRR"
     gene            1119..2141
                     /locus_tag="PLE93_00770"
     CDS             1119..2141
                     /locus_tag="PLE93_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH85608.1"
                     /translation="MSFSSSKKTRRSIQALLVLALVAFGAIIAGCGSSDGDSGSKVQL
                     VAYSTPQTAYEEGLEPAFNETPEGDGVEFSNSFGASGDQSRAVEAGQPAGYVNFALET
                     DVTRLVDAGLVSKDWQNNKYKGIVTNSVVSIVTRPGNPEGIESFQDIIDKDVSVVTPN
                     PFTSGGARWNIMAIYGAALHEGKSEQEALDTVKQVLSQAEVQPASARDALQAFVGGAG
                     DVLISYENEAIAAQNAGEDVDYVIPDSTILIEQPAAVTVDAPKSATDFLNYLWSDAGQ
                     KIWAEQGYRPVNKSLVDESVYPTPKDEFTIAEFGGWDKVADEFFDDKTGSIAEIEKEL
                     GVSTEG"
     gene            2206..2970
                     /gene="cysT"
                     /locus_tag="PLE93_00775"
     CDS             2206..2970
                     /gene="cysT"
                     /locus_tag="PLE93_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNH85609.1"
                     /translation="MTKGLVVTYLSLIVLIPLAAIAAKAFSAGPAEFWDAIRQPQAIA
                     ALKLTAICSLIVVAINAVMGTLIAWVLVRDEFPGKSIVNSMIDLPFALPTIVASLTLL
                     TLYGNDSFLGIHAAYTKFAVIIALLFVTLPFVVRAVQPLLLSLDRDMESAAESLGAGK
                     VRIFTQIIFPNLLPGIAAGVALAFARALGEFGALVLISGNVPYKTEVASVFIRGQIES
                     DQVVGAAAVSVVLLVFSMCMLAFVNLIQKWGLRHDR"
     gene            2960..3772
                     /gene="cysW"
                     /locus_tag="PLE93_00780"
     CDS             2960..3772
                     /gene="cysW"
                     /locus_tag="PLE93_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNH85610.1"
                     /translation="MTASGRLSKYGLRFVALFYLTALLLLPIGVALVKTFEDGIGEVI
                     KQITEPDAIHALKMSLLTVIIAVPLNTVFGIAAAHVVVRTKFRFKWAINALIDMPFAV
                     SPVVIGLALVLVYGTGGWFGADLAAMGIQVIFSTPGIVMAVIFVSLPFVAREVIPVMQ
                     EIGTDQETAAETLGASPWQTFRKITLPAIRWGVTYGIVLTTARALGEFGAVTVVSGNI
                     SGETQTLPLYVSKQFETFNSVGAYSASLILAVLALGTLLSMNLLRRREDQDD"
     gene            3765..4844
                     /locus_tag="PLE93_00785"
     CDS             3765..4844
                     /locus_tag="PLE93_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNH85611.1"
                     /translation="MTEITDHLNPESHGIEVREVNKEFGDFQALKDVNLIVPDGSMTA
                     LLGPSGSGKSTLLRTIAGPEVPTSGQVFIGGEDQARKPVRTRNVGFVFQHYAAFKHMT
                     VFDNIAFGLKVRKWDKQKTRDRVNELIDLVQLTGLAKRYPSQLSGGQRQRMALARALA
                     VEPSVLLLDEPFGALDAKVRKDLRQWLRRLHDEVHVTTIFVTHDQEEAMDIAEQIVVM
                     NNGQIEQTGSATDLYDRPENEFVMGFVGEVNSLGGRLVRPHDTEILPEPTEDTTEVEI
                     DRVVRLGFSGRVEMLTVPDKKEVWAQLTRSELETLSLEAGTRAYVRPRAEAVTKEDTG
                     ALEEALREESPATDTPIPAEPALPV"
     gene            complement(4783..5154)
                     /locus_tag="PLE93_00790"
     CDS             complement(4783..5154)
                     /locus_tag="PLE93_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH85612.1"
                     /translation="MTGSTDTWQTADGRTTRPALTEVCPHFHAGIELIGKRWSGAIIW
                     ALDDGPLRFAELKRSIPGLSDRLLSQRLRELEDAGLMTREVEDGPQVKVTYSLTEMGE
                     GLRPAVLALREWACRWQDSLP"
BASE COUNT          958 a         1817 c         1642 g          950 t
ORIGIN      
        1 cccggcgagt ggtacccgcc gcccgggcca ggcttgcgcg ggcggccggg atgtcgatcc
       61 cgaccacggc ttcggccgca tgggtcgggg ttgaggcccg aaccgccgcc acgtcgtcga
      121 gcagggtcac gtcccgttcg tgacccaccg cggaaatgac cggcaccgcg agcatcacga
      181 ccgtgcggca gagatcctcg tcgcagaagg cccagaggtc agtcaggctg ccgccgcccc
      241 ggcagacgac gatcgagtcc acatccgcga aagcggccat gtcactgagg atcctcgtga
      301 tctgcggagc cgccttgcgg tcctggaccg gggcaaagcc ccagaccacc tcgcccttcc
      361 agccgcgacg ctcgaggccg accatcagat cctgccgggc tgccccacct tccgcggtga
      421 tcaccccgat ccgccggggc agcaacctgc gcggcaggtt cttctgcggc tcggtcagcc
      481 cttccgcctg gaactgtttg cgaagcgccg caagacgggc gatcaggtcc ccctccccga
      541 acggacggat gtcgatggcc cggaaactga aggaaggcga agcgttcttg cctccctcgt
      601 aataatcggg cgcgccggtc acgactacct gcgaaccgac cttgacggtt cccgctggca
      661 ggccgagggc gtcgaactcc cgcttccaca tcgttcccgg caccccgccg cgttcgtcgc
      721 gcagctcgaa atagatctgg acccgtgact ggctgaagtt cgtgatttcg ccgaggatcg
      781 tcgcttcggg gaggcgcttc aggtcctgct tcagctggcg ggcatagtcg ccgaccgggg
      841 tcggcgcggc atcctctgcc gccgcagggt tgcccggttc cggaagctct tcgctcatcg
      901 ctccagcatg acaccgaccg ccgacgggcc tctgtgacgg atcggcacag gatcgtgctt
      961 ttggcttgaa acaggggaaa acccgaagat gccgacggta aatatgacta ttacgaccag
     1021 ttgactcgac attctctccg gacccgtgct accttgcccg tcgttatttg agtttgtagg
     1081 taaagaaagt aaccacatca acaagggggg aatcccgaat gagtttcagc agttccaaga
     1141 agacccggcg cagcattcag gcgcttctcg tcctggctct cgtagccttc ggcgcgatca
     1201 tcgccggttg cggcagcagc gacggcgaca gtggcagcaa ggtccagctg gtcgcctact
     1261 ccacccccca gaccgcctac gaagaaggtc tcgagccggc tttcaacgag acacccgaag
     1321 gtgacggtgt cgaattctcg aactcgttcg gagcttccgg cgaccagagc cgagccgtcg
     1381 aagccggcca gcccgccggg tacgtcaact tcgcgctcga gaccgacgtg acccgtctgg
     1441 ttgatgccgg cctcgtctcg aaggactggc agaacaacaa gtacaagggc atcgtgacca
     1501 actcggtcgt ctcgatcgtg acccgtcccg gcaacccgga aggcatcgag tccttccagg
     1561 acatcatcga caaggacgta agtgtcgtga ccccgaaccc gttcacctcc ggtggcgccc
     1621 gctggaacat catggccatc tacggtgccg ccctgcatga gggcaagagc gagcaggaag
     1681 cccttgacac cgtcaagcag gtcctctcgc aggccgaagt ccagccggcc agcgcccgtg
     1741 acgccctcca ggccttcgtc ggcggcgccg gtgacgtcct gatctcctac gagaacgagg
     1801 cgatcgccgc gcagaacgcc ggcgaggacg tggactatgt gattccggat tcgacgatcc
     1861 tgatcgagca gccggccgca gtcacggtcg atgccccgaa gtccgcgacc gacttcctca
     1921 actatctctg gagcgatgcc ggccagaaga tctgggccga gcagggttac cgccccgtca
     1981 acaagagcct ggttgacgag tccgtctacc cgacaccgaa ggacgagttc acgatcgccg
     2041 agttcggtgg ctgggacaag gtcgccgacg agttcttcga cgacaagacc ggctcgatcg
     2101 ccgagatcga gaaggaactg ggagtttcca ctgaaggctg aatccacagc cgtagcggca
     2161 cctcgacccc gggcgggcct gcgcctgccc ggggttgaaa ccgccttgac caagggcctg
     2221 gtcgtcacct acctgagcct gatcgtgctg atcccgctgg cggcgattgc ggccaaggct
     2281 ttctcggcgg gtccggccga gttctgggac gcgatcaggc agccgcaggc gatcgccgcc
     2341 ctgaagctga ccgcgatctg ctccctgatc gtcgtggcga tcaacgcggt gatgggcacc
     2401 ctgatcgcct gggtcctggt ccgggacgag ttccccggca agagcatcgt caactcgatg
     2461 atcgacctgc ccttcgccct gccgaccatc gtcgccagcc tcaccctgct cacgctctac
     2521 ggcaatgaca gcttcctcgg gattcacgcc gcctacacaa agttcgcggt gatcatcgcc
     2581 ctgctcttcg tgacccttcc cttcgtggtg cgggccgttc agccgctgct gctttccctt
     2641 gaccgtgaca tggagtccgc cgccgaatcg cttggcgccg gcaaggtccg catcttcacg
     2701 cagatcatct tccccaacct gttgcccggc atcgccgccg gcgtcgcact cgcctttgcc
     2761 cgcgcgctcg gtgaattcgg cgccctcgtg ctgatctcgg gcaatgtgcc ctacaagacc
     2821 gaggtcgcct cggtcttcat ccgtggccag atcgaatccg accaggtggt cggtgcggcc
     2881 gccgtctcgg tcgtgctcct ggtcttctcg atgtgcatgc tggccttcgt caacctgatc
     2941 cagaaatggg gtctgcgtca tgaccgctag tggccgactc tccaaatacg ggctccggtt
     3001 cgtcgcgctc ttctacctga ccgctctgct gctgctgccg atcggcgtgg ccctggtcaa
     3061 gaccttcgag gacgggatcg gtgaagtgat caaacagatc acggagccgg acgcgatcca
     3121 cgccctgaag atgtcgctgc tcacggtgat catcgcggtg ccgctcaaca cggtcttcgg
     3181 catcgccgcc gcccatgtgg tcgtgcggac gaaattccgg ttcaaatggg cgataaatgc
     3241 gctgatcgac atgccctttg ccgtctcgcc ggtggtcatc ggcctcgccc tggtcctcgt
     3301 ctacgggacc ggcggctggt tcggtgccga cctggccgcg atgggcatcc aggtcatctt
     3361 ctccaccccg ggaatagtca tggcggtgat cttcgtttcg ctgccgttcg tggctcgcga
     3421 agtaatcccg gtgatgcagg aaatcgggac cgaccaggaa accgccgccg agacgcttgg
     3481 cgccagcccc tggcagacct tccgcaagat caccctgccc gcgatccgct ggggcgtgac
     3541 ctacggaatc gtgctgacca cagcccgtgc cctgggcgag ttcggcgcgg tgacggtcgt
     3601 ctccggaaac atctccggcg aaacccagac gctgccgctc tacgtatcca aacagttcga
     3661 aacctttaac tcagtgggcg cctattcggc ttcgttgatc ctcgccgttc ttgcgctggg
     3721 caccctgctt tcaatgaacc tgctacgccg ccgagaggac caagatgact gaaatcactg
     3781 accacctcaa cccggaaagt cacggcatcg aggtccggga agtcaacaag gagttcggtg
     3841 acttccaggc cctcaaggac gtcaacctga tcgtccccga cggatcgatg accgcgctcc
     3901 tcggaccttc cggttcgggc aagtcaaccc tgcttcgcac catcgccggg cctgaagtgc
     3961 cgaccagcgg ccaggtgttc atcggcggcg aggatcaggc gaggaaaccg gtccggaccc
     4021 gcaacgtcgg tttcgtcttc cagcactacg ccgcgttcaa gcacatgacg gtgttcgaca
     4081 acatcgcttt cggcctcaag gtccgcaagt gggacaagca gaagacccgc gaccgggtca
     4141 acgagctgat cgatctggtc cagctgaccg gcctcgcgaa gcgttacccc tcgcagctct
     4201 ccggcggcca gcgccagcgc atggccctgg cccgggcgct cgcggtcgaa ccttcggtgc
     4261 tgctgctcga cgagcccttc ggcgcgcttg acgccaaggt ccgcaaggac ctgcgccagt
     4321 ggctgcgccg cctccacgac gaggtccacg tgaccacgat cttcgtcacc cacgaccagg
     4381 aagaggcgat ggacatcgcc gaacagatcg tggtcatgaa caacggccag atcgaacaga
     4441 ccggctccgc caccgacctc tacgaccggc ccgagaacga gttcgtgatg ggcttcgtcg
     4501 gcgaggtcaa cagcctcggc ggccggttgg tccgccccca tgacaccgag atcctgccgg
     4561 aaccgacaga ggacacgacc gaggttgaaa tcgaccgggt ggtgcggctg ggcttctccg
     4621 gccgggtcga gatgctcacg gttccggaca aaaaggaggt ctgggcgcag ctgacccgct
     4681 ccgagctgga gaccctcagc ctcgaagcag ggacgagggc ttacgtcagg ccacgggccg
     4741 aagcggtgac caaggaagac acgggcgcgc tcgaggaagc gctcagggaa gagagtcctg
     4801 ccaccgacac gcccattccc gcagagccag cactgccggt ctgagccctt cccccatctc
     4861 ggtgaggctg taggtcacct tcacctgggg gccatcttcg acctcgcggg tcatcaggcc
     4921 cgcgtcctcg agctcgcgca ggcgctggga gagcagccgg tccgaaagcc cggggatcga
     4981 acgcttcagt tccgcgaacc gcaggggccc gtcatccagg gcccagatga tcgcgccact
     5041 ccagcgcttg ccgatcagtt cgatgcccgc gtgaaaatgg gggcaaactt cggtcagggc
     5101 cggtcgggtg gtacggccgt cggccgtttg ccaggtatcg gttgaaccgg tcaccgttct
     5161 ttcactttag cggagccgga atgtgggaaa atccggattt cgaccgacgg gaggagatac
     5221 ccgcctacgc gcaaggtccg gtgtcgactt ccttgccgct gacttttttg ctgatgtcgg
     5281 caaggacccg gttcgggatt gccagcccgc cctgcggccc ttccgtggtt gcggcgaaca
     5341 ccgtgcccag cacctgcccc cgggaat
//
LOCUS       DAOQFI010000022         5317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6160_length_5317_cov_3.525276, whole genome shotgun sequence.
ACCESSION   DAOQFI010000022 DAOQFI010000000
VERSION     DAOQFI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6160_length_5317_cov_3.525276"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PLE93_00795"
     CDS             complement(<1..138)
                     /locus_tag="PLE93_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L16"
                     /protein_id="HNH85613.1"
                     /translation="MLMPKRVKHRKVMRGRRKGNSKGGTAVTFGEYGLKSLDRGWITN
                     RQ"
     gene            complement(138..860)
                     /gene="rpsC"
                     /locus_tag="PLE93_00800"
     CDS             complement(138..860)
                     /gene="rpsC"
                     /locus_tag="PLE93_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNH85614.1"
                     /translation="MGQKIHPEGFRVGYIHDWKSNWFADKDFSDLLMQDLAIRDHIEN
                     KLAHAGLSDITIERRGEVAVDIHTARPGIVIGKSGSEVDALRKDLHKITGSPVKVNIR
                     EVKRPELDAKLVAQSIAEQLQNRVAFRRAMKRALTSAMRSGAKGVKVQISGRLGGAEM
                     ARTEGYSDGRVPLHTLRADIDYGFVEAKTGTGRIGVKCWINKGEVMPEGFESSRTSDE
                     APMSDRPDRRGGRGDDRRGGRR"
     gene            complement(865..1248)
                     /gene="rplV"
                     /locus_tag="PLE93_00805"
     CDS             complement(865..1248)
                     /gene="rplV"
                     /locus_tag="PLE93_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNH85615.1"
                     /translation="MAAKTTRKEEAAEPVLVRATSKYVRVSPRKARLVADQVRGKHID
                     DARSLLQFSPRSVADDISKVIESAAANAEANHDLIGDEMIVAEIRVDEGPTLKRFRPR
                     AMGRATPIHKRTCHISVALTPEDEE"
     gene            complement(1248..1514)
                     /gene="rpsS"
                     /locus_tag="PLE93_00810"
     CDS             complement(1248..1514)
                     /gene="rpsS"
                     /locus_tag="PLE93_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNH85616.1"
                     /translation="MGRSTKKGPYVEERLMGRIEAMNEAGNKNMVKTWSRRSTIFPEM
                     VGHTIAVHDGRKHVPVFISESMVGHKLGEFAPTRTFKSHTGGKD"
     gene            complement(1518..2363)
                     /gene="rplB"
                     /locus_tag="PLE93_00815"
     CDS             complement(1518..2363)
                     /gene="rplB"
                     /locus_tag="PLE93_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNH85617.1"
                     /translation="MAIRKTKPTSPGRRFATYQLREQITATEPEKSLIEGIKKSGGRN
                     NNGRITSRHRGGGAKRRYRKIDFKRTKDGIPAKVATVEYDPNRSAYIALLHYADGEKA
                     YILAPSQIEVGNQVSSGPGSDIRPGNCLALGEMPVGTTVHNVELKPGQGGKLGRSAGT
                     AIQLVAKEGDMVTLRLPSSEMRMVRAECRATIGTISNAEHQNVKIGKAGRNRHKGKRP
                     QSRGIAMNPVDHPHGGGEAHKTPGGHPVTPWGKPTLGYRTRKKGKQSDRYIVRGRRRG
                     KKKGR"
     gene            complement(2387..2680)
                     /gene="rplW"
                     /locus_tag="PLE93_00820"
     CDS             complement(2387..2680)
                     /gene="rplW"
                     /locus_tag="PLE93_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNH85618.1"
                     /translation="MDPRSVIIRPVISEKAYALIAANKYTFRVDDRAHKFEIKDAVEK
                     AFGVEVVKVRTSKVRSKPKRRGLHQGRSRSWKKAIVELAPGDSIELFEGAAVE"
     gene            complement(2680..3336)
                     /gene="rplD"
                     /locus_tag="PLE93_00825"
     CDS             complement(2680..3336)
                     /gene="rplD"
                     /locus_tag="PLE93_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNH85619.1"
                     /translation="MASAKAPVLGKTTKADLPAGLFDEAFHQSLVHETARADANARRQ
                     GTASTLTRGEVSMTTAKAWRQKGTGRARVGALSVPNRYGGGAAFGPKPRHYTVKVNRK
                     ARRKAMRSALSVHAERGSVAVVKAADFETPSTRDAAAALEKWGAKRSTLVIILGEETG
                     LLKSFRNIPRVSVMEVGATGVVDVIGAASIVVSEAALEVLAAQSTEPDRGTGTEKDGE
                     "
     gene            complement(3329..4009)
                     /gene="rplC"
                     /locus_tag="PLE93_00830"
     CDS             complement(3329..4009)
                     /gene="rplC"
                     /locus_tag="PLE93_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNH85620.1"
                     /translation="MSAILGKKLGMTQLFREDGEVVPVTVIEAGPCPVTAIRNEERDG
                     YSAIQLAFDEVKESKLSKAEVGHLAKADAGPMRVLVEFRDVELPAAGSAGEEGSEGEE
                     AEGDGGEVKLGQQVTVEAFEEGQKVKVAGITIGKGFQGTVKRHNFGRGPVSHGSHNVR
                     APGSIGASAWPARVFKGMRLPGQMGGKRRTQLGLEIAGIDADRNLLMIKGSVPGAKNS
                     IVEIRTDG"
     gene            complement(4015..4326)
                     /gene="rpsJ"
                     /locus_tag="PLE93_00835"
     CDS             complement(4015..4326)
                     /gene="rpsJ"
                     /locus_tag="PLE93_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNH85621.1"
                     /translation="MAAQKIRIRLKAYDHDAIDRAAREIVDTAERTGATVSGPVPLPT
                     ERNIYCVIRSPFKDKDSREHFEIRTHKRLIDIKQPTPKTVDSLQRLDSLPAGVDIEIR
                     L"
     gene            complement(4632..>5317)
                     /locus_tag="PLE93_00840"
     CDS             complement(4632..>5317)
                     /locus_tag="PLE93_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNH85622.1"
                     /translation="PIIKGSALKALEGDADFQQRIVELGEALDSYIPEPERDLDKPFL
                     MPIEDVFSITGRGTVATGRIEQGIVNTGDEVEIVGIHDKTETTTITGVEMFRKILDEG
                     RAGDNVGCLLRGTKREEIERGQVLCKPGSITPHTKFRAEVYCLKKEEGGRHTPFFSGY
                     RPQFYFRTTDVTGVANLPEGTEMVMPGDNVEMTIELIQPIAMDQGLRFAIREGGRTVG
                     SGVVSEIIE"
BASE COUNT          772 a         1700 c         1777 g         1068 t
ORIGIN      
        1 ctgacggttg gtgatccagc cgcgatcgag ggacttgagc ccgtactcgc cgaaagtcac
       61 ggcagttccg cccttggaat taccctttcg gcggccccgc atgaccttgc ggtgcttgac
      121 acgcttcggc atcaacatca gcgacggcct cccctgcgat cgtcgccacg gccgccgcgg
      181 cggtcgggac ggtcgctcat cggggcctca tccgaggtcc gcgaggattc gaagccttcg
      241 ggcatgactt cgcccttgtt gatccagcac ttgacgccga tgcgcccggt gccggtcttc
      301 gcctcgacga agccgtagtc gatgtcggcg cgcagggtgt ggagcggcac gcggccgtcc
      361 gagtaaccct cggtgcgggc catttcggcg ccacccaggc gaccggaaat ctggaccttg
      421 acgcccttgg cgccggagcg catggcggag gtgagcgccc gcttcatcgc gcgccggaag
      481 gcgacgcggt tctggagctg ctcggcgatc gactgggcga ccagcttggc gtcgagctcg
      541 gggcgcttga cctcgcggat gttcaccttc accggcgagc cggtgatctt gtgaaggtcc
      601 ttgcggagcg cgtcgacctc gctgcccgac ttgccgatca cgatgcccgg acgggcggtg
      661 tgaatgtcca cggcgacctc gccgcgacgc tcgatcgtga tatcggacag gccggcgtga
      721 gcgagcttgt tctcgatgtg gtcgcggatc gccaggtcct gcatcagcag gtccgaaaag
      781 tccttgtcgg cgaaccagtt ggacttccag tcgtggatgt agccgactcg gaagccttcg
      841 ggatgaatct tctgtcccat gcttctattc ctcgtcttcc ggtgtcagcg ccacggagat
      901 gtggcaggtg cgcttgtgga tcggggtggc ccggcccatc gcccgcggcc ggaaccgctt
      961 cagggtgggt ccttcgtcga cccggatctc ggcgacgatc atctcgtccc cgatcaggtc
     1021 gtggttggcc tcggcgttgg cggcggccga ctcgatcacc ttggagatgt cgtcagcaac
     1081 cgaacgcggc gaaaactgca gcagcgagcg ggcgtcgtcg atgtgcttgc cacggacctg
     1141 gtcggcgacc aggcgggcct tgcggggtga cacgcgcacg tacttcgagg tggcacggac
     1201 cagtaccggc tcggccgcct cttccttcct ggtggtcttg gcagccatca gtccttaccg
     1261 ccggtgtggg acttgaaggt gcgggtcggg gcgaactcgc ccagcttgtg accgaccatc
     1321 gattcggaga tgaagacagg cacgtgcttg cgaccgtcgt ggacggcgat cgtgtggccg
     1381 accatctccg ggaagatcgt cgaacgacgc gaccaggtct tgaccatgtt cttgttgccg
     1441 gcctcgttca tggcttcgat ccggcccata agccgctctt ccacgtacgg gcccttcttg
     1501 gttgacctac ccacggtcta cctacccttc ttctttccgc gacgacggcc gcgcacgatg
     1561 taacggtcgg actgcttgcc cttcttgcgg gtgcggtacc cgagcgtggg cttgccccag
     1621 ggagtgaccg gatggccacc gggggtcttg tgtgcctcgc caccgccatg cgggtggtcg
     1681 accgggttca tggcgatgcc gcgcgactgc gggcgcttgc ccttgtgccg gttgcggccg
     1741 gccttgccga tcttgacgtt ctgatgctcc gcgttcgaaa tcgtgccgat cgtcgcgcgg
     1801 cattccgcac ggaccatccg catttccgag gaaggaaggc gcagggtgac catgtcaccc
     1861 tccttcgcga ccagctggat cgcggtgccg gcgctgcggc cgagcttgcc gccctggccg
     1921 ggcttcagct ccacgttgtg gacggtggta ccgaccggca tctcgccgag ggcgaggcag
     1981 ttgccgggac ggatgtccga gcccgggccg gagctgacct ggttgccgac ctcgatctgc
     2041 gagggcgcca ggatgtaggc cttctcgcca tccgcgtaat gcagcagggc gatgtaggcg
     2101 gagcggttcg ggtcgtactc gacggtcgcg accttggcgg ggatcccgtc cttggtccgc
     2161 ttgaagtcga tcttccggta gcggcgcttg gcccctccgc cacggtggcg ggaggtgatg
     2221 cggccgttgt tgttgcggcc acccgacttc ttgatgccct cgatcaggct cttctcgggc
     2281 tcggtcgcgg tgatctgctc acgcagctgg tacgtcgcga agcgacgccc ggggctcgtt
     2341 ggttttgtct tgcgaattgc cattactcag ttattccttc aagcccttac tcgaccgccg
     2401 cgccttcgaa cagctcgatg ctgtcgccgg gggcgagttc gacgattgcc ttcttccagg
     2461 agcggctgcg accctgatgc aggccacgac gcttcggctt tgaacggacc tttgaggtcc
     2521 ggaccttgac cacttcgact ccgaacgcct tctcgacggc gtccttgatc tcgaacttgt
     2581 gggcccggtc gtccacccgg aaggtgtact tgttggccgc gatcagggcg taggcctttt
     2641 ccgagatgac cggacggatg atcacgctgc ggggatccat cactcaccgt ccttttcggt
     2701 cccggttccc cggtccggtt cggtcgactg ggcggccaga acctcgaggg ccgcttcgga
     2761 aaccacgatc gaagcggcgc cgatcacgtc gacgactccg gtcgcgccaa cctccatcac
     2821 ggagacccgg gggatgttgc ggaagctctt cagcaggccg gtctcttccc cgaggatgat
     2881 caccagggtc gagcgcttcg cgccccactt ctcgagggcc gccgcggcgt ccctggtcga
     2941 aggggtctcg aagtcggccg ccttgaccac ggcgacgctg ccacgctcgg cgtgaaccga
     3001 aagggcggag cgcatcgcct tgcggcgggc cttgcggttg accttgaccg tgtaatggcg
     3061 cggcttcggc ccgaaggcgg cgccgccgcc gtagcggttc ggcacgctga gggcgccgac
     3121 ccgggcacgg cccgtgccct tctggcgcca ggccttggcg gtggtcattg aaacttcgcc
     3181 gcgggtcagc gtcgaggcag tgccctggcg ccgcgcgttg gcgtcggccc gggcggtctc
     3241 gtggaccagc gactgatgga aggcctcgtc gaagaggccg gccggcagat cggccttggt
     3301 ggttttgccc agaacggggg ccttggcgct agccatcggt acggatctcc acaatcgagt
     3361 tcttggctcc gggaacggag cccttgatca tcagcaggtt cctgtcggca tcgatgccgg
     3421 cgatctccag gccgagctgg gtgcggcgct tgccgcccat ctggccgggg aggcgcatgc
     3481 ccttgaacac gcgggcaggc caggcggacg caccgatcga gcccggggca cgaacgttgt
     3541 gcgaaccatg cgaaacggga ccgcggccga agttgtgccg cttgacggtg ccctggaagc
     3601 ccttgccgat cgtgatgccg gcgaccttga ccttctggcc ttcctcgaag gcctcgacgg
     3661 tgacctgctg gccgagcttg acctcgccgc cgtcaccttc ggcttcttcg ccttcggaac
     3721 cttcttcgcc ggccgagcca gcggcgggga gctccacgtc gcggaactcg accagcaccc
     3781 gcatcgggcc ggcgtcggcc ttggccaggt ggcccacttc ggccttgctg agcttcgact
     3841 ccttgacttc gtcgaaagcg agctgaatcg ccgagtagcc gtcgcgctcc tcgttgcgga
     3901 tcgcggtaac cggacagggt ccggcttcaa tgactgttac gggcacgacc tcaccgtctt
     3961 ctctgaacag ttgggtcatg ccaagctttt ttccgagaat cgccgacatg gtgcctacag
     4021 tcggatctcg atgtcgacgc cggccgggag cgagtccagg cgctgcagcg aatcgacggt
     4081 cttgggggtg ggctgcttga tgtcgatcag acgcttgtgg gtgcggatct cgaagtgttc
     4141 ccgagagtcc ttgtccttga acggcgaacg gatcacgcag tagatgttgc gctcggtcgg
     4201 cagcggcacg ggaccggaaa cggtcgcgcc ggtgcgctcg gccgtatcga cgatctcgcg
     4261 ggcagcgcga tcgatggcgt cgtgatcgta ggccttgagc ctgatcctga ttttctgtgc
     4321 ggcaatagcg gccactttgg cttctcttct tactttcttc tgctggcgca aatcagctgt
     4381 atcggctgag gcgctgatgt caaggtcccc cgccgcctgc ggctcagtcc ccgcccgtga
     4441 ggcggatcca gtgccgtggt tcgacaatcc ggggtacttc aaaggtcgta tctgttactg
     4501 gtgctgcgaa cttctttact tcgttactgc gttactgctg tgctgcggta cttcaggccc
     4561 tgagggcgca aaaacggggc ggacccgaaa acgtttgctg tcttcgggtc cgccccgggc
     4621 aaatcgtgtc gctactcgat gatctccgag acgacgccgg agccgaccgt gcggccaccc
     4681 tcgcggatcg cgaagcggag gccctggtcc atggcgatcg gctggatcag ctcgatcgtc
     4741 atctcgacgt tgtcacccgg catgaccatc tccgtgccct cgggcaggtt ggcgacgccg
     4801 gtcacgtcgg tggtccggaa gtagaactgc gggcggtagc ccgagaagaa cggggtgtga
     4861 cgaccgcctt cttccttctt caggcagtaa acctcggccc ggaacttggt gtgcggggtg
     4921 atcgagcccg gcttgcagag aacctggccg cgctcgattt cctcacgctt ggtgccacgg
     4981 agcaggcagc cgacgttgtc gccagcgcga ccctcgtcga ggatcttgcg gaacatctcg
     5041 acgccggtga tcgtggtggt ctcggtcttg tcgtgaatac cgacgatctc gacctcgtca
     5101 ccggtgttga cgatgccctg ctcgatacgg ccggtggcga cggtgccgcg accggtgatc
     5161 gagaagacgt cctcgatcgg catcaggaac ggcttgtcga ggtcacgctc cggctccggg
     5221 atgtaggagt cgagagcctc accgagctcg acgatgcgct gctggaagtc ggcgtcacct
     5281 tcgagggcct tgagggccga gcccttgatg atcgggg
//
LOCUS       DAOQFI010000023         5248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6302_length_5248_cov_3.781244, whole genome shotgun sequence.
ACCESSION   DAOQFI010000023 DAOQFI010000000
VERSION     DAOQFI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6302_length_5248_cov_3.781244"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3685
                     /gene="gltB"
                     /locus_tag="PLE93_00845"
     CDS             <1..3685
                     /gene="gltB"
                     /locus_tag="PLE93_00845"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNH85623.1"
                     /translation="MLAGRSLPHAAMMMIPESYQGRTDLSDELKGFYAFHSCLMEPWD
                     GPAAVCFTDGRVIGATLDRNGLRPGRWVETNDGMVVLGSEIGLLDIAPEDIKRLGRLQ
                     PGKLFLVDLEQKRIIEDEEVKREVATQQPYGEWFENASVHFDDLPTAYVTMTGIQPTP
                     RRQQAFGYTQEDLRVLITPMAATGAEPIGSMGNDVALPVLSDNRPPLYSYFKQLFAQV
                     TNPPIDPIREEIVMSLSAGVGAERNLLDESEQHAHQLCLDQPILMNQELETIRHVNHD
                     IFRADTIDITWDVAEGTAGIESRLDAICEEAEEAIEKGFNILILSDRRTGPRRVPIPA
                     LLAVGAVHHHLVRAGTRLRAGLILESGEPREVHHMATLIGYGCSAINPYLMLDSVDDL
                     VVNGLVPDMQSPEKAQRNVVKAIGKGLLKTISKMGISTTQSYGGAQIFEAVGIEKEVI
                     DKYFTGTASRIGGIGLDVIAQETMDRHASAFPGAGDDLLPVGGVYAWRRDGEFHQWNP
                     ETVSLVQHAVRGEGGSAQEKYDEFAQQVNEIALKRATLRGLFKLKAKDSGPVPIEEVE
                     PAKEIVKRFATGAMSLGSISRESHENLAIAMNRLGGKSNTGEGGEDPVRFTPEENGDS
                     RRSAIKQVASGRFGVTFHYLANADQLQIKMAQGAKPGEGGQLPGHKVDRYIGSVRHTT
                     PGVSLISPPPHHDIYSIEDLKQLIYDLCCSNPKASVSVKLVSEVGVGTVAAGVAKANA
                     DHVLIAGHDGGTGASPLSSVQSAGIPWEIGLAETQQTLIRNDLRSRITVQTDGQLKTG
                     RDVVVAAILGADEMGFSTAPLIASGCIMMRACHLNTCPVGIATQDPELRKRFKGQPEH
                     VVNYFFFVAEEARKIMAGMGVRSIDELIGRIELLETDDAVDHWKARGIDLSGLLAWPE
                     GIDHSVDRRRTRPQEPVLDDNLDWKILEQAGDAVENGIPVEFDMTVQNVDRCVGGITS
                     NAIANAQGAEGLPEDTIKVNFRGAAGQSFGGWLAPGITFSLRGEVNDYCGKGLSGGIL
                     AVSPPDGAAYQPEENVIVGNTSLYGATKGRAFFRGIGGERFAVRNSGATAVIEGVGDH
                     GCEYMTGGTVVVLGPTGRNFAAGMSGGIAYVLDEQDEFSGRVNPSMSNQLEEPDEKDR
                     IELRGLIQEHLERTGSEVARRILDDWESTSARFVKVFPEDYKRVLAELAAEGENEPGV
                     GPETNDVEVIGEPATGVASEKGA"
     gene            3689..>5248
                     /locus_tag="PLE93_00850"
     CDS             3689..>5248
                     /locus_tag="PLE93_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNH85624.1"
                     /translation="MGKLGGFLQIERAGIPYRDPAERVGDYNEFVVRRSDEELADQGA
                     RCMECGVPFCHNGCPLGNLIPDWNDLVYHGRWQDAIRQLHSTNNFPEFTGRLCPAPCE
                     AACVLEIREGEAVTIKQIEQSIADRAWKEGWIRPRPPKRETGRHVAVVGSGPAGMAAA
                     QQLRRGGHSVTLFERDEAAGGLVRFGVPDFKIEKTVVERRVQQLIHEGVEVRCGVEVG
                     VDVTVEELKERFDAVVLATGSRVPRDLPAPGRENDGVHFAMDYLYSRNRWLASEVGPK
                     PMTEAPAPAPISAKGKHVVVIGGGDTGADCVGHSIREGAAEVVQLELLPEPPEHRPDD
                     RTPWPLWPTKFRLSYAMEEAKGIEKGEQDFSVTTTSFNGEDGQVRSITVAKADPNPPF
                     AAVEGSEFEIKADLVLLAMGFLHPEQELLDGLGVDKDQRGNVKAGTYETSVPGVFAAG
                     DARRGQSLIVWAINEGRQAARMVDRFLGTLPSPSPVPRTPDVPTGNVNPADEGPEGPP
                     KHVAPGLGPDPS"
BASE COUNT          948 a         1804 c         1701 g          795 t
ORIGIN      
        1 gatgctggcc ggtcgctcgc tgccgcatgc ggcgatgatg atgatccccg agtcctacca
       61 gggccggacg gacctgagtg acgagctgaa gggcttttac gcgttccact cctgcctgat
      121 ggagccgtgg gacggaccgg cggcggtctg cttcaccgac ggccgcgtga tcggcgccac
      181 gctcgaccgc aacggtctga ggcccggccg ctgggtcgaa accaatgacg gcatggtcgt
      241 gctcggctcc gagatcggcc tgctcgacat cgcccccgag gacatcaagc gcctcggccg
      301 cctgcagccc ggcaagctct tcctcgtcga tctcgagcag aagcggatca tcgaggacga
      361 ggaagtcaag cgggaagtcg ccacccagca gccctacggc gaatggttcg agaacgcctc
      421 ggtccatttc gacgacctgc cgaccgcgta tgtgacgatg accgggatcc agccgacccc
      481 gcgtcgccag caggccttcg gctacaccca ggaggacctg cgggtcctga tcaccccgat
      541 ggccgcgacc ggtgccgaac cgatcggctc gatgggcaac gatgtcgcgc tgccggtcct
      601 ctccgacaat cgtcccccgc tttacagcta cttcaagcag cttttcgccc aggtcaccaa
      661 cccgccgatc gatccgatcc gcgaggaaat cgtgatgagc ctctcggccg gggtcggcgc
      721 cgaacgcaac ctgctcgacg aaagcgagca gcacgcccat cagctctgtc tcgaccagcc
      781 gatcctgatg aaccaggaac tcgagacgat ccgccacgtc aaccacgaca tcttccgcgc
      841 ggacacgatc gacatcacct gggacgtcgc cgaaggcacg gccggcatcg agtcccgcct
      901 tgacgcgatc tgcgaagagg ccgaggaagc gatcgagaag ggcttcaaca tcctgatcct
      961 ttccgaccgc cggaccggcc cgcgccgcgt cccgatcccg gccctgctcg cggtcggcgc
     1021 ggttcaccat cacctggtcc gggccggcac gcgcctgcgg gcgggcctga tcctcgagtc
     1081 gggcgagccg cgcgaggtcc atcacatggc gaccctgatc ggctacggct gctcggcgat
     1141 caacccctac ctgatgctcg actcggtcga cgacctggtc gtaaacggtc tcgtgccgga
     1201 catgcagtcg ccggagaagg cccagcgcaa tgtagtcaag gcgatcggca agggccttct
     1261 gaagacgatc tcgaagatgg gcatttccac cacccagtcc tacggtggcg cccagatctt
     1321 cgaagcggtc ggcatcgaga aggaagtgat cgacaagtac ttcaccggca cggcctcgag
     1381 gatcggcggc atcggtctcg acgtgatcgc ccaggagaca atggaccgtc acgcctcggc
     1441 gttccccgga gccggcgacg acctgctgcc ggtcggcggt gtgtacgcgt ggcgtcgtga
     1501 cggcgagttc caccagtgga acccggagac ggtgtcactg gtccagcacg cggtccgcgg
     1561 cgaaggtggc tccgcccagg agaaatacga cgagtttgcc cagcaggtca acgagatcgc
     1621 cctgaagagg gccaccctgc gcgggctctt caagctgaag gcgaaggatt cgggtccggt
     1681 tccgatcgag gaagtcgaac ccgctaagga aatcgtgaag cggttcgcga ccggcgccat
     1741 gtcgctcggc tcgatctccc gcgaaagcca cgagaacctg gcgatcgcca tgaaccgact
     1801 cggcggcaag tcgaacaccg gcgagggcgg ggaggacccg gtccggttca ccccggaaga
     1861 aaacggcgac tcgcgccgct ccgcgatcaa gcaggtcgct tccggccggt tcggggtcac
     1921 cttccactac ctcgcaaacg ccgaccagct ccagatcaag atggcccagg gcgccaagcc
     1981 cggcgagggc ggccagctgc ccggccacaa ggtcgaccgc tacatcggct cggtccggca
     2041 cacaaccccg ggcgtcagcc tgatctcgcc gccgccgcac cacgacatct actcgatcga
     2101 ggacctcaag cagctgatct acgacctctg ctgctcgaac ccgaaggcct cggtttcggt
     2161 caagctggtt tccgaagtcg gagtcggcac ggtcgcggcc ggcgttgcca aggcaaatgc
     2221 cgaccatgtg ctgatcgccg gccacgacgg cggcaccggc gcctcaccgc tttcgtcggt
     2281 ccagtcggcc gggatcccct gggagatcgg cctggcggaa acgcagcaga ccctgatccg
     2341 caacgacctc cggtcccgga tcacagtcca gaccgatggt cagctgaaga ccggccggga
     2401 cgtcgtcgtc gccgccatcc tcggcgccga cgagatgggc ttctcgaccg ctccgctgat
     2461 cgcctcgggc tgcatcatga tgcgggcctg ccacctcaac acctgcccgg tcggcattgc
     2521 cacccaggat ccggagctgc gcaagcgctt caagggccag cccgagcacg tcgtcaacta
     2581 cttcttcttc gtggccgagg aggcccggaa gatcatggcc gggatgggcg tacggtcgat
     2641 cgatgaactg atcggccgga tcgagctgct cgagaccgat gatgcggtcg accactggaa
     2701 ggccaggggc attgacctct ccggcctcct ggcctggccc gaggggatcg accactcggt
     2761 cgaccgccgc cggacccgcc cgcaggagcc ggtcctcgac gacaacctcg actggaagat
     2821 tctggaacag gcgggggacg cggtcgagaa cggcatcccg gtcgagttcg acatgaccgt
     2881 ccagaacgtc gatcgctgcg tcggtggcat cacctccaac gcgatcgcca acgcgcaggg
     2941 cgccgagggt cttcccgagg acacgatcaa ggtcaacttc cgcggcgccg ccggccagtc
     3001 cttcggcggc tggctcgcgc ccggcatcac cttctcgctg cggggcgagg tcaacgatta
     3061 ctgcggcaag ggcctttccg gcggcatcct cgcggtgtca ccgcccgatg gcgccgccta
     3121 ccagccggaa gaaaacgtga tcgtcggcaa cacctcgctc tacggtgcga ccaagggccg
     3181 ggccttcttc cgcggcatcg gcggcgagcg cttcgccgtc cgcaactccg gtgccaccgc
     3241 ggtgatcgag ggcgtcggcg accacggttg cgaatacatg accggcggca cggtggtcgt
     3301 gctcggtccg accgggcgca acttcgcggc cgggatgagc ggcggcatcg cctacgtgct
     3361 cgacgagcag gacgagttca gcggacgggt caacccgtcg atgagcaacc agctcgagga
     3421 accggacgag aaggaccgga tcgaactccg cggcctgatc caggagcacc tcgagcgcac
     3481 cggttccgaa gtcgcccgcc gcatcctcga tgactgggag tcgacctcgg cgaggttcgt
     3541 caaggtcttc cccgaggatt acaagcgggt actggccgag ctcgcagccg aaggcgagaa
     3601 cgagccgggg gtcggacccg aaaccaacga cgtcgaagtc attggcgagc cggccaccgg
     3661 tgtcgcctcg gaaaagggag cctgagcaat gggcaagctg ggaggtttcc tccagatcga
     3721 acgtgccgga atcccctacc gggatcccgc cgaaagggtc ggcgactaca acgagttcgt
     3781 ggttcgcaga tccgatgagg agctggccga ccagggcgcg cgctgcatgg aatgtggtgt
     3841 ccccttctgc cacaacggct gcccgctcgg caacctgatc ccggactgga acgacctcgt
     3901 ctaccacgga cgctggcagg acgcgatccg tcagctccac tcgaccaata acttccccga
     3961 gttcaccggt cgcctttgcc cggccccctg cgaagccgcc tgcgtgctcg agatccgcga
     4021 gggcgaagcg gtaacgatca agcagatcga gcagtcgatc gccgaccggg cctggaagga
     4081 aggctggatc cggcctcgtc cgcccaagcg cgagaccggc cgccacgtcg ccgtggtcgg
     4141 ttcgggcccg gccggcatgg ccgccgccca gcagcttcgc cggggcggcc acagcgtcac
     4201 cctcttcgag cgggatgaag ccgccggcgg cctggtccgc ttcggagtac ccgacttcaa
     4261 gatcgaaaag accgtggttg aacgccgcgt ccagcagttg atccacgaag gcgtggaggt
     4321 caggtgcggg gtcgaagtcg gggtcgatgt gaccgtcgag gaactgaagg aacgcttcga
     4381 tgcggtcgtg cttgccaccg gttcccgcgt tccccgtgac cttccggcgc cgggccgcga
     4441 aaacgacggt gtccatttcg cgatggacta tctctacagc cgcaatcgct ggctggcgtc
     4501 cgaggtcggt cccaagccga tgaccgaggc cccggcaccg gccccgattt cggcgaaggg
     4561 caagcatgtc gtggtgatcg gcggtggcga caccggcgcc gactgcgtcg ggcattcgat
     4621 ccgcgagggc gcggccgagg tggtccagct cgaactgctg cccgagccgc ccgagcatcg
     4681 cccggatgac cgcaccccgt ggccgctctg gccgaccaag ttccgcctct cctacgcgat
     4741 ggaagaagcg aagggaatcg aaaagggcga acaggacttc tccgtgacca cgacctcctt
     4801 caacggggag gacggccagg tccggtcgat cacggtcgcc aaggccgatc cgaacccgcc
     4861 tttcgccgcg gttgaaggca gcgaattcga gatcaaggct gacctggtcc ttctggcgat
     4921 gggcttcctc catccggagc aggaacttct cgacggtctc ggtgtggaca aggatcagcg
     4981 cggcaacgtg aaggcaggca cctacgaaac ttcggtgccc ggcgtctttg ccgccggtga
     5041 tgcccgtcgc ggccagtcgc tgatcgtctg ggcgatcaac gaaggccgcc aggccgcccg
     5101 catggttgac cgcttcctcg ggaccctgcc ttcgccgtca ccggtgccgc gcacgcccga
     5161 cgtgccgacc ggcaacgtca atccggccga cgaaggcccg gaaggaccgc caaagcacgt
     5221 cgccccgggc cttggccccg atcccagc
//
LOCUS       DAOQFI010000024         5246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6304_length_5246_cov_3.294934, whole genome shotgun sequence.
ACCESSION   DAOQFI010000024 DAOQFI010000000
VERSION     DAOQFI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6304_length_5246_cov_3.294934"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(894..1175)
                     /locus_tag="PLE93_00855"
     CDS             complement(894..1175)
                     /locus_tag="PLE93_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85625.1"
                     /translation="MEELNEQQIAALLRELPAPPQHLVERAKMIPRVNRQMEQVLPKF
                     ETLEKGRRVETAELEKAIEAAGLEHDKVLIDALKERLEQETDDQDGGTP"
     gene            complement(1209..1769)
                     /locus_tag="PLE93_00860"
     CDS             complement(1209..1769)
                     /locus_tag="PLE93_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH85626.1"
                     /translation="MLRAGSLSDGDLVNRCLDGDDEAWAVFVERFSSFVYAILMRGFR
                     LSTHEAEDVFQEVFIRAFDRLDTVRDADAVKSWLAQITRRLAVDRIRSGGREVVVEEL
                     PEQTDDGFYARLDEAMDVREALEAMGGNCRDILDRFFIRDQTYRTIGEELEIPPGTIA
                     SRISRCLSNLKDQLEEPETDLAEEGR"
     gene            complement(1795..2370)
                     /locus_tag="PLE93_00865"
     CDS             complement(1795..2370)
                     /locus_tag="PLE93_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85627.1"
                     /translation="MDENDADGLAGIPLGEILTRISADADGLAAGSTAAISLAFAAGL
                     VESVARTYLGEGSPASLVAQAETVRDRAIDVAGRNRVDYARARAALSGKTETDDLRLR
                     EALDSTLENLGWIAGHAADLTMLAAQLVGKVDPDREPDLESAVCLAESAARVSIKLAD
                     ANLLNSPAMPLLDEIAEDLRIVVSTREKLDH"
     gene            complement(2370..4055)
                     /locus_tag="PLE93_00870"
     CDS             complement(2370..4055)
                     /locus_tag="PLE93_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85628.1"
                     /translation="MEVRIANSGYRALLSATAVLILFLAWPGSEARGSGFAALTTGAT
                     HGCALRSSGEALCWGANSEGQLGDGGTEKELIPTPVEGLADATSISAGNSHTCAIRGS
                     GQVVCWGSNGNGQLGDGLSDHLTPEPVPGLNDATSISAGDSHTCAIRSSGQAVCWGYN
                     GWGQIGDGTTDDSLTPTAVPGLTDATEIAAGWSHSCAIRTGGQVVCWGGNASGQIGDG
                     TTDDQLTPTPVTGLTDAVSIAVGQGQSCAVRTSGQAVCWGDNSGGQLGDGTTDDRLTP
                     TAVIGLTDAGEITAGGSHTCAIRTTGQAVCWGTNRDGQLGDGTSSGDEDPDGIPTPVS
                     SLSNALNIAAGDSYTCAIRGIDQAVCWGKNNGRLGDGTLQDRLLPTAVSDLPPPVIGV
                     TVNLVKIKGKVTTKCPGERRFRKLIKPRQVKVGCRLDTRKGTVSLTSAKAKKSGTQAG
                     RFWGGLFRVGQTRRKTLTTMNLTGSTKCPVNAPHRRPIRRGWGKDKGGKFRIGGHGGS
                     ASSRGTHWGVVDRCDGSTKVLVKEGSVSVRDFVRKKTVTVRAGHSYVTGKRKR"
     gene            complement(4480..>5246)
                     /locus_tag="PLE93_00875"
     CDS             complement(4480..>5246)
                     /locus_tag="PLE93_00875"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH85629.1"
                     /translation="GTFQVMYLLLAFQRQQTRDRFSRFVPESVVEQVLADGSGDLKLG
                     GVRTEATVLFSDIRNFTTYSESRPPEDVVEVLNHYLGLMTDTIMNHGGTLISYMGDGI
                     MAVFGAPVAQPDHADRAISGAREMLNKSLPTFNEWMKQAGYGDGFRIGIGVNTGEVMS
                     GQVGSERRMEYTTIGDTVNTAARLEGMTKGSGFEILVSETTREACTRTVSDLDEIGER
                     EVRGRDEAIRLWGLRAVEEKTKGRDKEDSLVPFRLD"
BASE COUNT          986 a         1709 c         1494 g         1057 t
ORIGIN      
        1 atggatggcg gtgtcgatgc tccccacagt gagggagcgc atcatcgttt cggggagttt
       61 ggagatctcc gaatcccttc cagtaaggac ttccgtgcca ccgctcccag gcttgcaatc
      121 tgaggacgga ccgtccccgt tccgccacct ggcttatcgg acacatcgat cgccacgaac
      181 ggtccctccg catcgacagc catgagcgaa accggcaggt agtcgagcac cccgccgtca
      241 agcagaagct ttctcggtta ccccgaggtc ctcaacgcag gcgtccgcct tcctgtcaac
      301 cggttcccat cctcccgatg gtttacagac tccggtatcg gcgcttggtg atcacaaatc
      361 cgtgtctcga gcgattgaca cagacgactt tcctcctgtt cgcgcggcaa gcaaccccga
      421 tcctccgata tgaccatttc tgaccgaccc tgagggttgg caagaacttt ggagaccgtg
      481 gcggcgcctg ccaggtgtac gggctgatca ttgccctgtc aggtcggccg ttgccgctga
      541 ggaaaacaaa gccgggatct ccgtccggag gcgggtttgc gggtggcggg atcacccgcg
      601 cctgacacaa gatctggctg gccggttgat tctgcaagtg aaatacaact ccgcaaatca
      661 ctttcccgtc aagtgccttc cagagcaaga cgtgagaagt cctgcgatgc acggccgaac
      721 ccgaccgatc accgattgcc cgggccccga ccggcgagga ggcggaagcg tcagcctgcg
      781 accggcccgc gaagcttccg aacccgagag caagcaggac cgccagcgcg acaagcgcca
      841 ctgcggacaa ggatgagttc cactgtcttg gttcagtcat tgccgcggta ctttcatggt
      901 gtgcctccat cctggtcgtc tgtctcctgt tcgagtctct ccttgagagc gtcgatcagg
      961 actttgtcgt gttcgaggcc ggccgcctcg attgcctttt ccagttcggc ggtttcgacc
     1021 cggcgtcctt tttccagcgt ctcaaacttt ggcagcacct gctccatctg ccgattgacc
     1081 cgagggatca ttttggcccg ctcgaccaag tgttggggtg gggcaggcag ttcccgtagg
     1141 agtgccgcga tctgttgttc gttcagttct tccatggtcg gtcagagtca agacggaact
     1201 ggtgccgtct atcttccctc ctcggccaaa tcggtttccg gctcctccag ttgatccttc
     1261 agattggaga ggcaacggga aatccggctg gcgatggtgc cgggcgggat ctccaactcc
     1321 tcgccgatgg ttctgtaggt ctggtcccga atgaagaacc ggtcgaggat atcccgacag
     1381 ttccctccca tcgcctccag tgcctctcga acatccatgg cctcgtcgag acgcgcgtag
     1441 aacccatcgt cggtctgttc cggcaactcc tcgactacca cttcacgacc accggagcgg
     1501 attcgatcca ccgccagccg gcgggtgatc tgggccagcc aggacttgac tgcgtccgcg
     1561 tcgcggaccg tgtcgagccg atcgaaagca cgaatgaaca cttcctgaaa gacgtcctcg
     1621 gcctcgtggg tagacagccg gaagccgcgc atgaggatcg cgtacacgaa cgaagaaaag
     1681 cgctcgacga agacagccca ggcctcatca tccccatcca gacaacgatt gaccaaatcc
     1741 ccatcgctga ggcttccagc cctgagcata gggcgagcct agcagcgaaa tcggtcagtg
     1801 atccagtttt tcccgcgtcg aaacaacgat tctcaggtct tcggcgatct cgtccagcaa
     1861 gggcattgcc ggtgaattca gaagatttgc atcggccagc tttatcgaga cccgggcggc
     1921 cgactccgcc aggcacacgg cagactccag atccggctcg cgatcggggt cgaccttgcc
     1981 gacgagttgt gcggcgagca tggtcaggtc ggcggcatgg cccgcgatcc atcccagatt
     2041 ctccagagtg gagtcaagcg cctcacgcag ccgcagatca tcggtctccg ttttaccgga
     2101 caaagccgcc ctggcccgag cgtaatcaac ccggttgcgg cccgccacgt cgatcgcgcg
     2161 atccctgacc gtttcggctt gggcgaccag tgacgcgggc gaaccttccc ccagatatgt
     2221 gcgagcgaca gattcgacca agcccgccgc gaaggccaga gagatcgctg ctgtggagcc
     2281 ggccgccaga ccgtcggcgt ccgccgagat tcgagtgaga atttctccaa gaggaatacc
     2341 ggccagaccg tcggcgtcat tctcatccat caacgcttcc tctttccggt gacgtaggaa
     2401 tggccggccc tgacagttac cgtcttcttg cggacgaagt ccctgacgct gaccgaaccc
     2461 tccttcacca gcactttggt cgaaccatcg caacgatcca ccacgcccca gtgagtacct
     2521 cgcgaactcg ccgatccacc gtggccaccg atccggaatt tgcccccctt gtccttgccc
     2581 caacccctcc ggatcggacg acgatgggga gcattcacag gacacttcgt cgaccccgtc
     2641 aaattcatcg tggtgagtgt cttccttcgc gtctggccaa ccctgaacag tccgccccag
     2701 aacctgccgg cctgggtgcc cgacttcttt gccttcgcgg aagtgaggct caccgttccc
     2761 ttccgggtat cgagccgaca gcccactttg acctgacggg gcttgatcaa tttcctgaat
     2821 cggcgctcgc cagggcactt ggtggtgact ttgcctttga tcttcacgag gttgacagtc
     2881 acgccgatga ccggaggcgg cagatccgat acggccgtcg gcaaaagacg atcctggagc
     2941 gtgccgtcac cgagccggcc gttgttcttg ccccagcaga cggcttgatc gatgcccctg
     3001 attgcgcagg tgtatgagtc tcccgcggcg atgttcaacg cgttcgacag ggagctgacg
     3061 ggcgttggga taccgtcggg atcctcgtca ccgctggagg taccgtcacc cagttgtcca
     3121 tcccgattgg taccccagca gacggcctga ccagtggttc gtatcgcaca tgtgtgtgaa
     3181 ccaccggcgg taatttcgcc ggcgtcggtg aggccaatca cggccgtcgg ggtgaggcgg
     3241 tcgtcagtag tgccgtcgcc aagctgcccg cctgagttgt cgccccagca gacggcctgc
     3301 cccgaggttc gaacagcgca gctttgtccc tggccgacgg cgatcgacac cgcgtcggtc
     3361 aggccggtca ccggggtcgg ggtcaactgg tcgtcagtgg tgccatcacc gatctggccg
     3421 gatgcattgc cgccccagca gacgacttgc ccgccggtcc ttatcgcgca gctgtgagac
     3481 cagccggctg cgatttcggt ggcatcggtc aagcccggga ccgcggtcgg agtgagactg
     3541 tcatcggtgg ttccgtcgcc gatctggccc catccgttgt agccccagca aaccgcttgg
     3601 cccgagcttc gaatcgcgca ggtgtgcgaa tcgccggccg agatagaggt cgcgtcattc
     3661 agccccggca cgggttccgg ggtgagatgg tcggacaaac catcaccgag ctggccgttg
     3721 ccgttgctgc cccagcaaac cacctgaccg gaaccccgga tcgcgcatgt atgtgaattg
     3781 ccggcactga tcgaagtcgc atcggccagc ccttcgacgg gagtcggaat gagctccttt
     3841 tcggtgccac catcaccgag ctggccttcc gaattggcac cccagcacag agcctcaccg
     3901 gaacttcgca gagcacaacc gtgagtagcg ccggtggtca gggccgcgaa tccggacccc
     3961 cttgcttctg atcctggcca ggccagaaag agaatcagaa ccgccgtcgc ggagaggaga
     4021 gccctgtaac ccgaatttgc gatccgaacc tccatccgat cctaggcgaa gggcagctga
     4081 gttccgaaga ccattatccg ccactcgtaa cgatttacct tgggtccgtg tgaacaacca
     4141 agtgcgccac cggcaccgct tgagcaacgc cagcgacctg gcaggattga ataccaccca
     4201 cttcgaccgg tccccggtgg acgcacgttc ctgccaccgc cttcaccgta ggtcttgatc
     4261 agccggcgag ccctgtggca cgaaacgtga ccccggtaaa tggtcacttt gtaagcaatg
     4321 ccgttgaccc gaatccgacc gcagttggtc atccccgccg aagccgggct cgttggcagt
     4381 ccggaaaaga aaagtgccgc gacgattgcg gctatcccga tgatggttct tcgcatgatg
     4441 cctgctcctt gttgcagatg gccagattca tcacttctgt cagtccagac gaaagggaac
     4501 cagggaatct tccttgtccc gaccctttgt cttctcttcc acggccctta gtccccagag
     4561 cctgattgct tcgtcccgtc ccctcacttc ccgctctccg atttcgtcca ggtctgacac
     4621 cgtacgcgtg cacgcctctc tggttgtttc ggatacgagg atctcgaatc ccgatccttt
     4681 ggtcatacct tcgagccggg ctgcggtatt cacagtgtcg ccgatcgtcg tgtattccat
     4741 ccggcgctcg gaaccaacct ggcctgacat cacctcgccg gtgttcacgc caatcccgat
     4801 gcgaaatccg tcaccgtagc cagcttgctt catccattcg ttaaaggtgg gcaggctctt
     4861 gttgagcatt tcgcgagctc cggaaatcgc tcggtccgca tgatcgggct gtgccaccgg
     4921 agctccgaaa acggccatga tgccgtcccc catgtacgag atgagggtgc ctccgtgatt
     4981 catgatcgtg tcggtcatca gccccaggta gtgattcaga acctcgacga catcctccgg
     5041 gggccgactc tcggaatacg tggtgaagtt acggatatca ctgaacagga ccgttgcctc
     5101 ggtccgcaca ccgcccagtt tgaggtcgcc cgagccatcg gcgagcacct gctcgacgac
     5161 ggactccgga acgaacctgg aaaatctgtc tcgggtttgc tggcgctgaa acgccagcag
     5221 cagatacatg acctggaagg ttccca
//
LOCUS       DAOQFI010000025         5196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6426_length_5196_cov_3.272904, whole genome shotgun sequence.
ACCESSION   DAOQFI010000025 DAOQFI010000000
VERSION     DAOQFI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6426_length_5196_cov_3.272904"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLE93_00880"
     CDS             complement(<1..339)
                     /locus_tag="PLE93_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNH85630.1"
                     /translation="MALRSIWNGTISFGLVKVPIKLYSATEPKGIAFREIHVKDNSLL
                     QHRRVCKAEGREVDRSEIAKGYEVRPDQYVVLESDEVKAAAGERPKTIEIEEFVDVEA
                     IDPFYFNKSYF"
     gene            432..1340
                     /gene="ligD"
                     /locus_tag="PLE93_00885"
     CDS             432..1340
                     /gene="ligD"
                     /locus_tag="PLE93_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNH85631.1"
                     /translation="MKARLGDPGKGVAWNFEVKWDGYRAIAFCSGGLKLQGRRLNEIG
                     ADFPEIKPLGSDSRARGLVLDGELVVVDEDGRPDFQLMQSRRERQLEAHFMIFDLLWA
                     DGTDLRPMPYLERRARLEALGLTGPTWSVPERLAGELNEVLEATAALGLEGVVAKDPD
                     SPYVSGRRTNYWLKVKHVKRQEFVIGGWLPGKGHRSGSLGSLLVGYYDGNSELRLAGR
                     VGTGMGDELLAELFATLSASARVDSPFVAEDAASIPRGAGWCEPELVIEVRFTEWTRE
                     GRLRNPVFVGFRPDKSPREVVREEPA"
     gene            1337..2293
                     /gene="ligD"
                     /locus_tag="PLE93_00890"
     CDS             1337..2293
                     /gene="ligD"
                     /locus_tag="PLE93_00890"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNH85632.1"
                     /translation="MTFGWKRSERLTSAVTGGPGKASIEIDGRELRLTNLDRVLYPET
                     GFTKGDLIDYYAAVAPLLLPHLRDRPVTMRRFPDGVESEGFWEKHCPDHRPGWVKTAS
                     IESSSSSKGRVDYCLVNDVATLVWMANLGCIELHVSLARARRRKTPDFMVFDLDPGPP
                     ADILDCAETALLIHDTLAGQDLVSLAKVSGSKGIQVYVPLNGRASYDRTGGFAHSLAR
                     AFEQELPDQIVSRMTKSLRKGKILIDWSQNSEHKTTVSVYSMRARSSPSVSMPVGWER
                     LEQALDRSDDSLLRFGPAEVLRRISGTDPFGAILELKQKLPG"
     gene            complement(2355..2777)
                     /locus_tag="PLE93_00895"
     CDS             complement(2355..2777)
                     /locus_tag="PLE93_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNH85633.1"
                     /translation="MSKTAREVMTGGAECIGESESITRAATRMAELDVGSMPICGEDD
                     RLKGMLTDRDIVVKVIAEGKDPDEVTAGEFGQGKPVTVGADDSVEVAIETMKEHAVRR
                     LPVIDGHELIGIVSQGDLACEVSEKAAGDLVEAISSAP"
     gene            2959..3174
                     /locus_tag="PLE93_00900"
     CDS             2959..3174
                     /locus_tag="PLE93_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85634.1"
                     /translation="MVKVGIAIGLVFLVAALSVFGSPFFAVLLLVPAGILAFAFLGGA
                     VVAEETGPEDSGKEPGPPKTSEAGQQN"
     gene            3247..3957
                     /locus_tag="PLE93_00905"
     CDS             3247..3957
                     /locus_tag="PLE93_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85635.1"
                     /translation="MIEAIGWGAVAASSLVLGVLLAFARPWSDRLVGSVLAFGAGALI
                     SAVSFSLAGEGLDIGSPPSVGAGLAVGALAYYLASRFIDRPRPGERADSSASGRDLAL
                     GSFLDGIPEQLVLGIGIVSGSGISLALLMAIFVSNLPEGIGSAADMERAGASRAYVLR
                     LFILIAIACAIAAAVGYLLADFISNELQACLDGFAAGALLVMMIDSMIPEAREKAGNV
                     AGLLTVVGFAAATALSGA"
     gene            complement(3960..4421)
                     /locus_tag="PLE93_00910"
     CDS             complement(3960..4421)
                     /locus_tag="PLE93_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85636.1"
                     /translation="MKFRETAIKALVLIGAVATCLAFTVPATSARSSKPPVFWIDLSG
                     TAQQHPDFVFFSANAGSQVNHLKWTGWGKNRAVGRGHYYVTSPPIPGEKNPEGAARIV
                     VWKPVKCVPDFGNREGKTIHIYKHARMLRPVSGGGRKWVDISAFAGAATCR"
     gene            complement(4522..4824)
                     /locus_tag="PLE93_00915"
     CDS             complement(4522..4824)
                     /locus_tag="PLE93_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNH85637.1"
                     /translation="MADSNGNTHGYVHDKERYLARLKRIEGQARGIHRMIEEDKYCID
                     ILTQISALTSALEGVAIGLLDEHMKHCVVDAAREGGPEAEAKIKEASDAIARLVRS"
     gene            complement(4814..>5196)
                     /locus_tag="PLE93_00920"
     CDS             complement(4814..>5196)
                     /locus_tag="PLE93_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNH85638.1"
                     /translation="MVGDGVNDAPALAQADLGLAMGTGTDVAIEAADITLVRGDLRSV
                     GEAIRLSRRTLGTIKVNLFWAFAYNVAAIPLAAFGLLNPMLAGAAMAFSSVFVVGNSL
                     RLRSFGRGREEPAGNIEKGTGNGR"
BASE COUNT          839 a         1713 c         1646 g          998 t
ORIGIN      
        1 gaagtaggac ttgttgaagt agaagggatc gatcgcctcg acatcgacga actcctcgat
       61 ctcgatcgtc ttcggccgct cgccggccgc cgcctttacc tcatcactct ccagcacgac
      121 gtactggtcg gggcgcacct catagccctt ggcgatctcg cttcggtcaa cttccctgcc
      181 ctccgccttg cagacccggc ggtgctgcag cagcgaattg tccttcacgt gaatctcgcg
      241 aaaggcgatt cccttcggtt cggtcgcaga gtacagtttg atcgggacct tgaccagccc
      301 gaaggagatg gttccgttcc agattgaccg caacgccatg cggtgagcgt acccgcgaac
      361 cgcttcatcc acaccatcgc gggtattcgg tagacatgat gcaagcgatg ttgcccggtc
      421 caatcgaacc catgaaagcc cggctcggcg atcccggcaa gggggtcgcc tggaactttg
      481 aagtcaagtg ggacggttac cgggccatcg ccttctgttc cggaggcctg aaactccagg
      541 gccggaggct caacgagatc ggcgccgact ttcccgaaat caagccgctc ggttccgact
      601 cccgcgcccg cggcctggtc ctcgacggcg agctggtggt agttgacgag gatggccggc
      661 cggacttcca gttgatgcag tcccgacgcg agcgtcaact cgaagcgcat ttcatgatct
      721 tcgacctgct ctgggccgac ggcaccgacc tcaggccgat gccctacctc gaacgccgcg
      781 cgcggctgga ggctctcggt ctaacgggcc cgacctggtc ggtcccggaa cggctcgccg
      841 gagagctgaa cgaggtgctc gaagccacgg ccgcactcgg gctcgaaggg gttgtcgcca
      901 aggatcccga cagcccctat gtgtcgggcc gacggacgaa ctactggctc aaggtcaagc
      961 acgtcaaacg gcaggaattc gtgattggcg gctggctgcc cggcaagggt caccgttccg
     1021 gttcgctggg ttcgctcctg gtcggctact acgacggcaa ctccgaactc cggctggccg
     1081 gcagggtcgg caccggaatg ggtgacgaac tgctggccga actcttcgcg accctttctg
     1141 cttcggcccg ggtcgactcc ccgttcgtcg cggaagacgc tgcctcgata ccccggggcg
     1201 ccggctggtg tgaaccggaa ctggtgatcg aggtcaggtt caccgagtgg acccgcgaag
     1261 gacgactgcg gaatccggtc ttcgtcgggt tcaggccgga caagtcgcca cgggaagttg
     1321 tccgggagga gccggcatga ccttcggctg gaagcgcagc gaacgactca cctcggcggt
     1381 caccgggggc ccgggcaagg catcgatcga gatcgacggc agggaactca ggctgacgaa
     1441 tctcgaccgg gtgctctacc cggagaccgg tttcacaaag ggcgacctga tcgactacta
     1501 cgccgccgtg gcccccttgc tcctccccca tctccgcgac cgtcccgtca ccatgcgtag
     1561 gttccccgac ggggtcgaaa gcgaaggctt ctgggagaag cattgtcccg accatcgccc
     1621 cggctgggtc aagactgcct cgatcgagag cagctcatcc tcgaaggggc gggttgacta
     1681 ctgcctggtc aacgacgtcg ccaccctcgt ctggatggcg aacctgggat gcatcgaact
     1741 ccatgtttca ctggcccggg ccaggcgccg gaagacgccg gacttcatgg tcttcgacct
     1801 cgacccgggc ccgccggctg acatcctcga ttgtgccgag actgccttgc tgatccacga
     1861 cacgctcgcc ggccaggatc tcgtctcgct ggcgaaggtc tcgggctcaa agggaatcca
     1921 ggtttacgtt ccgctcaacg ggcgggccag ctacgaccgg accggaggtt tcgctcattc
     1981 actggcccgg gccttcgaac aggaattgcc ggaccagatc gtttcgcgga tgacgaaaag
     2041 cctgcgcaag ggaaagatcc tgatcgactg gagccagaac tccgagcaca agaccaccgt
     2101 ttcggtgtac tccatgcggg ccaggagcag cccgtcggtt tcgatgcccg tcggctggga
     2161 gcgactcgag caggcgctcg accgctcgga cgactcgctg ctccgcttcg ggcccgcgga
     2221 agtgcttcgc aggataagcg gcaccgatcc gttcggcgcc atcctcgagc tgaagcagaa
     2281 gctgcccggc tgacccggga gagggagtca aggattgggg gcgccgccct tgccacggcg
     2341 acgccccggg cgggtcaggg tgcggaggaa atcgcttcca cgagatcccc ggccgccttc
     2401 tcggagacct cacaggcgag gtcaccctga ctcacgattc cgatcagttc atgtccgtcg
     2461 atcaccggga gccgccttac cgcgtgctcc ttcatggtct cgatcgcgac ttcaaccgag
     2521 tcgtcggctc cgacggtgac gggcttaccc tgaccaaact cgccggcggt cacctcgtcg
     2581 ggatcctttc cttccgcgat caccttcacg acgatgtcgc ggtcggtcag cattcccttc
     2641 aggcggtcgt cttcgccgca aatcggcatc gatcccacat caagttcggc catccgcgtg
     2701 gcggcgcgag tgatgctctc cgactcgccg atgcattcgg caccgccggt cattacttcc
     2761 cgtgctgtct tgctcatctc ctgcctcctg tctgggtggt ttgcccctcc gccgaccgct
     2821 gtggcggagt tcaactagag gcgtaccccc agccggagcg tccaaactca aagattggga
     2881 gatccggtgt cgggtacctg ccgaagcgtt cccgaaagtt ggccggtcgc gatttcgcgg
     2941 cgggccggaa aggcgtcgat ggtcaaagtt ggcatcgcta ttggactcgt tttcctcgtc
     3001 gcggctctct ccgtattcgg atcgccgttc ttcgcagtcc tgcttctggt cccggccggc
     3061 attctcgcct ttgccttcct gggcggagcg gtagtggccg aggagaccgg tcccgaagat
     3121 tccgggaaag agccgggtcc gccgaagacc tccgaggcag gccagcagaa ctgaccccgg
     3181 gcccgggttc ggtatccgcc gcacccgtct agagtccccg ccgatggccg tggcggagac
     3241 tttccgatga tcgaggcgat cggctggggt gccgttgccg cgtcttctct ggttctcggc
     3301 gttctgctcg cgttcgcgcg gccctggtcc gaccgcctgg tcgggtccgt gcttgccttt
     3361 ggcgctggtg cgctgatcag cgcggtcagt ttcagcctgg ccggcgaggg cctggacatc
     3421 ggcagcccgc catcggtcgg cgccggtctc gcggttggag cgcttgccta ctaccttgcg
     3481 agccgtttca tcgaccggcc ccggcccggc gaaagggccg attcgtcagc cagcggccgg
     3541 gaccttgccc tcgggtcctt cctcgacggg attccggaac agctggtgct gggtatcggc
     3601 atcgtttccg gatccgggat cagcctggcc ctgctgatgg cgatcttcgt ttcaaacctg
     3661 ccggagggaa tcggttccgc cgccgacatg gagcgggcgg gtgcctcgcg ggcgtacgta
     3721 ctccggctgt tcatcctgat cgcgattgcc tgcgcgatcg ctgcggcggt cggttacctg
     3781 ctcgccgatt tcatctcgaa cgaactccag gcctgcctgg atggtttcgc cgccggcgcc
     3841 ctgctggtga tgatgatcga ctcgatgatt cccgaagcaa gagaaaaggc cggcaatgtt
     3901 gccggccttc tcacggtcgt cggttttgcc gcagcgactg cgctgagtgg cgcctgaact
     3961 tacctgcagg tggccgcgcc ggcgaaggcg gagatgtcga cccacttgcg ccctcccccg
     4021 gaaacgggtc gcagcatcct cgcgtgctta tagatgtgaa tcgtcttgcc ctcgcgattg
     4081 ccgaaatccg ggacgcactt gaccggcttc cagacgacga tccgcgccgc gccttccggg
     4141 ttcttttcac cggggatcgg cggcgaggtc acgtagtagt ggccgcggcc gaccgcgcgg
     4201 ttcttgcccc agccggtcca cttgaggtga ttgacctggg agccggcatt cgccgagaag
     4261 aaaacgaagt cgggatgctg ctgggcggtt ccggagaggt cgatccagaa gaccggaggc
     4321 ttggaggacc tggccgaggt ggccggaacg gtaaacgcga gacaggttgc gacggcaccg
     4381 atcagcacca gcgccttgat tgcggtctcc ctgaacttca ttgaataccc cctgttgatt
     4441 ggtcggagcc cgtgatgata tccgctgctg cgcccggcct caatttcgaa gcagtgaggc
     4501 aagggccggt cgcgaccgat ttcacgacct gaccaggcgg gcgatcgcgt cggaggcttc
     4561 cttgatcttc gcctcggctt ccgggcctcc ctccctcgcc gcgtcaacca cgcagtgctt
     4621 catgtgctcg tcgagcaggc cgatcgccac tccttccagg gccgaggtaa gcgccgagat
     4681 ctgggtgagg atgtcgatgc agtacttgtc ctcctcgatc atccggtgga tgccgcgagc
     4741 ctggccttcg attcgtttca ggcgagccag ataccgctcc ttgtcgtgaa cgtacccgtg
     4801 ggtgtttccg ttgctatcgg ccattgcccg ttcctttctc gatgttgccg gccggttcct
     4861 ccctgccccg cccgaacgat ctcagccgca ggctgttacc caccacgaag acgctggaga
     4921 aggccatcgc cgccccggcc agcatcgggt tgagcaggcc gaaggccgcc agcgggattg
     4981 ccgccacgtt gtaggcgaag gcccagaaga ggttcacctt gatcgtgccg agagtacgcc
     5041 gggaaagccg gatcgcctcg cccacgctcc tcagatcccc acggaccagg gttatgtcag
     5101 ccgcctctat cgccacgtcg gtgccggtgc ccatcgcgag cccgaggtcg gcctgggcga
     5161 gcgcgggcgc gtcgttgacg ccgtcgccga ccatcg
//
LOCUS       DAOQFI010000026         5169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6477_length_5169_cov_3.010559, whole genome shotgun sequence.
ACCESSION   DAOQFI010000026 DAOQFI010000000
VERSION     DAOQFI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6477_length_5169_cov_3.010559"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLE93_00925"
     CDS             complement(<1..312)
                     /locus_tag="PLE93_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNH85639.1"
                     /translation="MAVQTNTPEAVFQAVRENDVRFVRLWFTDVLGNLKSFSINSSEL
                     ERAFEQGIGFDGASLTGFNPVEESDMVARPDASTFSLIPWRPGGTDAVARMFCDIQVP
                     GG"
     gene            526..1092
                     /locus_tag="PLE93_00930"
     CDS             526..1092
                     /locus_tag="PLE93_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNH85640.1"
                     /translation="MTVKTEVSRFHIHDELTAPDESAPILKSIQVGGRQVNKLVGVLA
                     GSPAVLRAYTRMRAELQQGELPQATRQRIALAVAEHRGDAYSVVQYAKSARNAGLGLD
                     EVSRARSFTSSDPKEQALLTYLKAALEADGDPPIYLHEEAREAGWDDEEILEALAHVA
                     MGEFQSVMAAAAALPKDQATPRVLPDAA"
     gene            complement(1237..2040)
                     /locus_tag="PLE93_00935"
     CDS             complement(1237..2040)
                     /locus_tag="PLE93_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85641.1"
                     /translation="MSTKTFPTVGRRTQAAAAFAGLIALIAGLAFMSGPGNQAGAASG
                     AAAAAGKAVYLGKASAKRIPLCPQNCQSVVIVSGFQAKAGGVGNAYRVPFIGNITRWR
                     LKLGKPTAKDRQFFQDRFGAVPQAAIGVLAKREQNGKIVYKLRRRSAIQNLNRFLGKT
                     ATFNLAAPIKVNKGDYVALIVPTWAPALAVPDACSIVRVNGKDQMKNPSVCRQFNASN
                     SWVASRDRTTCTQKVTMRNSQPQIDVNSLASYGCRFNGALTYGVRVESR"
     gene            complement(2209..2904)
                     /locus_tag="PLE93_00940"
     CDS             complement(2209..2904)
                     /locus_tag="PLE93_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HNH85642.1"
                     /translation="MERSHRLGKLNIAVVAGILLVLALVVVLAGSGNDKAAASTGGIG
                     LASSKKSQARTSSSLVRGARLGARTLREGMSGPDVKVLNSIVRFKNFLSGAATPTFSR
                     PTTSAVRRFQNSADMASSGVVNKTTARALIGSLHTSEASWYGPGLYGNGTACGKVTLR
                     PTTIGLANKNLPCGTRVLIGYRGKFIIAPVIDRGPYGPGRTWDLTKAAADAVGLTSAG
                     VGRVRHAILSRGY"
     regulatory      complement(2911..3064)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            3304..3697
                     /gene="rnpB"
                     /locus_tag="PLE93_00945"
     ncRNA           3304..3697
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLE93_00945"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4163..4840)
                     /gene="lexA"
                     /locus_tag="PLE93_00950"
     CDS             complement(4163..4840)
                     /gene="lexA"
                     /locus_tag="PLE93_00950"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNH85643.1"
                     /translation="MVDLTKRQREIFDYIGKYTDKMGYPPTVREIGQAVGLTSSSTVH
                     VHLANLEKAGVLRRDPSKPRALEVLVGKAKSAGGSVRDSARDVVDSVATAASNVAATP
                     SGLPLLGRVSAGPGILAEENIEDYVDVPGMIGGSDGDYVLEVRGESMKNAGIMDGDYV
                     IVHPSPEARDGEIVVALLGDEDATVKRFYKETDHFRLEPENETMSSIITTDAQVMGKV
                     IGVFRKV"
     gene            5044..5130
                     /locus_tag="PLE93_00955"
     tRNA            5044..5130
                     /locus_tag="PLE93_00955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5078..5080,aa:Leu,seq:gag)
BASE COUNT          934 a         1627 c         1673 g          935 t
ORIGIN      
        1 accgcccggt acctggatgt cgcagaacat gcgggcgacc gcgtcggtcc cgccgggtcg
       61 ccagggaatg aggctgaagg tcgaagcgtc gggccgggcg accatgtccg attcctcgac
      121 cggattgaaa cctgtcagcg aggccccgtc gaagccgatc ccctgctcga aggcccgttc
      181 gagttcgctc gaattgatcg agaagctctt caggttgccc agcacgtcgg tgaaccagag
      241 gcggacaaaa cggacgtcgt tttccctgac tgcttgaaag acggcttccg gggtatttgt
      301 ctgtactgcc atcggactcc ttggaggggc taaataagcg gcttcgagcc ggaatcgagg
      361 tgcttcaggc ggaaacgata cgcgaggggc ttgcgtaacg ccgccggagt gggtataact
      421 tacttgcggt aagtaactag ctaaaagtaa agtaatggct cgttaaacgg atggtaaagg
      481 ccggagacga gccgtaactc ggagaacact gggaagcgtg acatcgtgac cgtaaagaca
      541 gaagtcagcc gattccatat ccacgacgaa ctgaccgctc ccgacgagag cgcgccaatt
      601 ctcaagagca tccaggtggg cggccgccag gtgaacaagc tggtcggcgt gctggccggc
      661 tcccccgccg tgcttcgcgc ctacacccgc atgcgtgcgg aactccagca gggtgagctt
      721 ccccaggcca cccggcagcg gatcgctctc gcggtggccg aacacagggg tgatgcctac
      781 agcgtcgtcc agtacgcgaa gtctgcccgc aacgccggcc tcggcctcga tgaagtgtcg
      841 cgggcccgca gcttcacttc ctcggacccg aaggagcagg cgctgctcac ctacctgaag
      901 gccgcgctcg aagcagacgg cgacccgccg atctaccttc acgaggaagc ccgcgaggcc
      961 ggttgggatg acgaagagat tctcgaagcc ctggctcacg tggcaatggg tgagttccaa
     1021 agcgtgatgg ctgcagccgc agccctcccg aaagatcagg caactccgcg ggttctgccc
     1081 gacgccgcct gatcacctcc ataaaaggac cctcccccgg ggtccgatcg aggcggcccc
     1141 tctctctccc ctggccgacc tctcccccta acaaaaaagg acggccccga tcggggccgt
     1201 cctttctttc gtacctctcg aaactgtgtc gaagcgctag cggctctcga cccggacgcc
     1261 gtatgtcagg gcgccgttga accggcaacc gtatgacgcc aggctgttga cgtcgatctg
     1321 cggctgcgaa ttcctcatcg tgactttctg ggtgcaggtc gtgcgatcgc ggctggcgac
     1381 ccagctgttg ctcgcgttga actgcctgca gacgctcggg ttcttcatct ggtccttgcc
     1441 gttcacccgg acgatcgagc aggcgtcggg aacggcgagg gccggagccc aggtcgggac
     1501 gatcagggcg acgtaatcac ccttgttcac cttgatcggg gcggccaggt tgaaggtcgc
     1561 ggtcttgccc aggaaccggt tcaggttctg gatcgccgag cggcgacgga gcttgtagac
     1621 gatcttgccg ttctgctcac gcttcgcgag cacgccgatc gcagcctgtg gaacggcgcc
     1681 gaaccggtcc tggaaaaact ggcggtcctt ggcggtgggc ttgccgagct tcaggcgcca
     1741 gcgggtgatg ttgccgatga aggggacccg gtaagcgttc ccgacgccac cggccttcgc
     1801 ctggaaacca ctgacgatga ccacggactg gcaattctgc gggcaaaggg gaatgcgctt
     1861 ggcgctcgct ttgccgagat agaccgcctt gccagcggcg gcagcggcac cactggctgc
     1921 gccggcctgg ttgccggggc cgctcatgaa agccaggccg gcgatcaggg cgatcaggcc
     1981 cgcaaacgcg gcggcggcct gcgtgcggcg gccgacggtc gggaaggtct tcgttgacat
     2041 cagtttcact agctccttgt tgtaagtctc gcggcgcaag tcaggtattt ggcgcggcgg
     2101 gtcacggtac aaacagtcga aaaaccgcga tggatcggtt tttccctgca aagaaaaggt
     2161 cgccaagggg tttcgccccc cgccgacgcc tgatttccgg tgatgcggtt agtagccgcg
     2221 gctgagaatc gcgtggcgga cccggccgac gccggcgctg gtcaggccta ccgcatcggc
     2281 agcagccttg gtcagatccc aggtgcgtcc gggcccgtag ggaccacggt cgatgaccgg
     2341 cgcgatgatg aacttgccgc ggtagccgat cagcaccctg gtgccgcaag ggaggttctt
     2401 gttggcgagg ccgatcgtgg tcggacgcag ggtgaccttg ccgcaggcgg tcccgttgcc
     2461 gtagaggccc gggccgtacc aggaagcttc ggaagtatgg agcgacccga tcagcgcccg
     2521 ggcggtcgtc ttgttgacaa ccccgctgct ggccatgtcg gccgagttct ggaagcggcg
     2581 gactgcgctg gtggtggggc gactgaaggt cggggtggcc gcgccggaga ggaagttctt
     2641 gaagcggacg atgctgttca gcaccttcac gtcggggccg gacatgccct cgcgaagcgt
     2701 ccgggcgccg agccgggctc ccctgaccag gctggaagaa gtccgggcct gggacttctt
     2761 ggaagaagcg aggccgatgc caccggtcga ggctgctgcc ttgtcgttgc cgctgccggc
     2821 cagaaccacg accagggcca gcacgagaag aatcccggcg acgactgcga tattcagttt
     2881 gcccagccga tggctgcgtt ccattccttc ttctctcttt cgtaaggctt gcggggttag
     2941 ctgtcgggct cgcgctgaaa gagcagcgct acctggcgga tgccagtgat tcgcccctgg
     3001 accgatgtcc tttgggtccc ccgttctccg cgatcttgag gcggagattc agcagaaggt
     3061 tcgggaagat tagcaaacac tcacgttatg tgtgcatttc atttgtgtga tgtacgtccg
     3121 tttcttgaaa cgagccgcga gaactccggg cggggaccgg aggtaccgcc tcctcggccc
     3181 gaaacgtcgt cggcgccacc tccgatcccc gcaaagtggc gcgccgatca gtgccgcatg
     3241 ccggatcgac atgggcgctc ccccactttc agcccgccgc taccaggccg gcggtacaat
     3301 tcatctgtgg gtcggacggc tgcggggcga gagaaaacgc ttcgaggaaa gtccggacac
     3361 cgaagggcaa ggacggtggg taacgcccac ccggggaaac ccgcgggaaa gtgccacaga
     3421 aatacacggc cgatggcgcg accacacgtg agtcgcgatc aggcaaaggt gaaaaggtgc
     3481 ggtaagagcg caccggcgtc gtggtgacac ggcggccagg taaaccccgt ccggtgcaag
     3541 accaaacagg atcgtcgacc cggcccggcg agatccaggt aggtcgcagg caagtccggg
     3601 attcgctcag cgaaccccgg caggcccacc ggtccgatca accggtgaga tagatggtcg
     3661 ttcaagacag gatccggctt acagacccac taaggaagag gccccctcgg gggccttttc
     3721 tacttgcatc aaagcttcca ggtaacgccg ctttgcccag gtcggtggtt acgccgtggg
     3781 gccgggtggg ggtagccggg acggacgcac gcgcggcatt cagccgcgct ggtcctggag
     3841 tgtcccggag acccccaccc gatcccacgg ctacacgaac ccgggaggtc cccacccgac
     3901 cccgcggctg ccatggccct cccgacgcga ttcgcgagag tccacttttg gccgcatagc
     3961 gcactcaact ccgatctcga atcggcgggc cggatcgaga tcggagttgg gtgcgctatt
     4021 tgccaataac tcccgcctcg aattcccccg agattccgac ccactccccc ttcaccagcc
     4081 cctgcagact tcaaggtgac gcaggtttgc gacctctgcg gttgcaaacc tgcggcacct
     4141 ttcagaggcg gaccccccgt gcctagacct ttcggaaaac tccgatcacc ttgcccatga
     4201 cctgggcatc ggtggtgatg atcgagctca tcgtctcgtt ctccggttcg agacggaaat
     4261 ggtccgtttc cttgtagaag cgcttgaccg tggcgtcttc atcgccgagc agggcgacca
     4321 cgatctcgcc gtcgcgggct tcaggtgagg ggtggacgat cacgtagtcg ccgtccatga
     4381 tgccggcgtt cttcatgctt tcgccgcgaa cctcaagcac gtagtcgccg tcgctgccgc
     4441 cgatcattcc gggcacgtca acgtaatcct cgatgttctc ctcggcgagg atgccgggac
     4501 cggccgaaac ccgcccgagc aggggcaggc cggaaggggt agcggccaca ttgctggcgg
     4561 cggtggcgac gctgtcaacc acgtcacggg cgctgtcccg gacgctgccg ccggcgctct
     4621 tcgccttgcc gaccagcacc tcgagggccc ggggcttgct cgggtcacga cgcaggactc
     4681 cggccttctc gaggttggcg agatgcacgt ggacggtcga tgaggaagtg aggccgactg
     4741 cctggccgat ttcccggacc gtgggcgggt aacccatctt gtccgtgtac ttgcctatgt
     4801 agtcgaatat ttcgcgctgc cgcttggtca gatcaaccac gacgctcctt ttcggctctc
     4861 ggccttctgt tgggaagcgg tccggcccgg agtggcgaac ctgtgttcgt aatgtacccc
     4921 tccttgcgga cgtatgttcg tgccggggcg aaaaccgcaa cctgacgacc agaatgcgac
     4981 cgaatcgaat ccgggtcagc ctgccgccgc ctgccccctt cccctagaat ccgcggtcct
     5041 cttgcggctg tggcggaatt ggtagacgcg caagcttgag gggcttgtgt ccgcaaggac
     5101 gtggaggttc gactcctctc agccgcacca tagtttcgac gtgagaacct ccacgcccag
     5161 agatgggct
//
LOCUS       DAOQFI010000027         5165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6483_length_5165_cov_3.137769, whole genome shotgun sequence.
ACCESSION   DAOQFI010000027 DAOQFI010000000
VERSION     DAOQFI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6483_length_5165_cov_3.137769"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLE93_00960"
     CDS             <1..219
                     /locus_tag="PLE93_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85644.1"
                     /translation="GASASQELGDRLSPREPKTSQELGELFAGLDPDRQQTIGLAIIR
                     RETAVMTLASFVIAFLIWRALDTTAGMF"
     gene            complement(484..1488)
                     /locus_tag="PLE93_00965"
     CDS             complement(484..1488)
                     /locus_tag="PLE93_00965"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNH85645.1"
                     /translation="MDARRRVSPAELNRKLGQVVKSGHGVITGDDLREIGVSRQTIRR
                     RKESGLLIPVLRDVFTLPGCRPDEIGRRKAAVHAGGSSCCLSHRSALAHHGLIYDQLP
                     VHVLRTGGADRRNGSALKRSRDFGFSVLSHQTRNLPPDEITIIEGIRVATPERALLDF
                     AATGRGSDIGKVLTQGERKGNLCWGRLESILENSRGRRGVTTLREEMDLFDPESVNSE
                     SDPEEDFLRMIRRRSLPMPLVNVWLAPYRVDFLWWHLMLIVELDPYGTHRGKRSFHGD
                     RRKSVELETRGFRVIRLTEKDLYAFEERTARDLETIMEAQAALLGVSVWPAGRNAATR
                     "
     gene            complement(1852..2769)
                     /locus_tag="PLE93_00970"
     CDS             complement(1852..2769)
                     /locus_tag="PLE93_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNH85646.1"
                     /translation="MSSHPFFWLDVFAESPLAGNGLAVVLDADGVSDEIMLAFARETG
                     LSETTFVQSPSSEAASSGADYRNRIWTIATEVPFAGHPSLGTAVAVALDRGVEEITFT
                     QETGAGLQQVEVSRAGAADPSPEGGEAAAGSGVWRASVLQEPASFGEAVPRDRAARLL
                     NLDESRLHPDYSAQVVSTGFPTVLLPLRNVEALADAVPDRAEFESFEFGDLAVNVYPF
                     VFDVPTRSVRARCFALDLGDFEDPATGSAAGPLVAYLQDILDVDRVEIHQGVEIGRPS
                     LIEARMDSGRPRVSGRVHVVMTGTATLPG"
     gene            complement(2807..3301)
                     /locus_tag="PLE93_00975"
     CDS             complement(2807..3301)
                     /locus_tag="PLE93_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNH85647.1"
                     /translation="MAAPDRNEFRQTVGLLPTGVTVVSAFQASGPAGATASAVCSLSL
                     EPMMMLVCLDLGSRSLEAVREAGRFGISVLGESQRAAAEMFATKADQEEKWSSVVWRE
                     HLEVPIIEGSLARVVCDLAEVIPGGDHVIVTGEVLEVEAEDEPGEPLIYHGGLFRRLD
                     AQQD"
     gene            3334..3780
                     /locus_tag="PLE93_00980"
     CDS             3334..3780
                     /locus_tag="PLE93_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH85648.1"
                     /translation="MRAVAITTLAVAAALGLSACGFGEEGITVSKDSPDYEGAQLFAA
                     NCAGCHTLTPAGALGTGNRGTRNQGPNLDQRAVTYEDALHAIQNGGFSGAIMPQNIVV
                     GEEAEQVARFVAEYAGSDAGEAGAEAYSAEEDASSPQDEPIPTDGQ"
     gene            3796..5061
                     /gene="serS"
                     /locus_tag="PLE93_00985"
     CDS             3796..5061
                     /gene="serS"
                     /locus_tag="PLE93_00985"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNH85649.1"
                     /translation="MLDLKLIRSDPERIKAALARRGAADQVDALLTLDERRRQLLPEI
                     EGARSERKQESEKIGALKKAGEDASAQMEAVRQLKARLEEMEAELEAVDGKLREVAAT
                     LPNLPDPTSPDGYTDEDAELIREVGEIPDFGFEPRDHVELAGDSIELEAAARVSGARF
                     AYLKGDLVMLEFALVRWALEKLRAEGHEPVVPPILVREEALFGTGFLPGDRDQIYELE
                     KDELFLAGTSEVPLAGLHADQILEADQLPLRYAGFSSCFRREAGAAGRDTHGIFRVHQ
                     FDKVEMFSFVKPEDAGAEHERILAIEESILTELGLPYRVVNVAVGDLGGSAAKKYDCE
                     AWIPSQGRYRELTSCSNTTDFQARRIGSRYRPEPGAPPEFVNTLNGTAVAVGRTIIAL
                     LENHQQADGSVRLPESLVSYGAPAVIGGN"
BASE COUNT          947 a         1719 c         1700 g          799 t
ORIGIN      
        1 ggcgcatccg ccagccagga acttggggac cggctcagcc cgcgggaacc gaagaccagc
       61 caggagctcg gcgagctctt cgccggactc gacccggacc gccagcagac gatcggcctg
      121 gcgatcatcc gccgcgaaac cgcagtgatg actctcgcca gtttcgtgat cgccttcctc
      181 atctggcgcg ccctggatac caccgccgga atgttctaaa cccctccctc gaagcatcct
      241 gaaacgaggc agacgccagt ggaggcactc gcctcgtgtc ggcacccacg tcgcggccac
      301 actcacctgg tgccggcgtc ctgacacgag gcaggcgcca gttgcgacgc tcccgtcgtg
      361 gcggcactcg cctcgtgtcg gcacccacgc cgcggccaca ctcacctcgt gccggcgtcc
      421 tgacacgagg caggcgccag tcgcggggct cgcgtcgtgt cgggactcgc ctcgtgtcgg
      481 ggctcacctc gtggcggcat tccgtcccgc cggccagacc gagacgccga gtagcgctgc
      541 ctgggcctcc atgatggtct cgaggtccct cgcggttctt tcctcgaacg cgtagagatc
      601 cttctcggtc agcctgatga ccctgaagcc ccgggtttcg agttcgacac tctttcgacg
      661 atcgccgtgg aatgacctct tgccccgatg cgttccatac ggatcgagtt cgacgatcag
      721 catcaagtgc caccaaagga agtcgacccg gtatggggcc agccagacat tcaccagggg
      781 catcggaagg gaccgccgcc ggatcattcg aagaaagtct tcttccgggt cggactcgga
      841 gttgactgat tcgggatcga aaaggtccat ttcttcccgc agcgtggtca ctccccggcg
      901 gcctcgagag ttctcaagga tcgactcgag cctgccccag cacaggtttc ccttgcgctc
      961 cccctgggtc aggaccttcc ctatgtccga tcccctgccg gtcgcggcaa aatcgagcag
     1021 ggcacgctcc ggagtcgcaa cccggattcc ctcgatgatc gtgatctcgt ccggcggaag
     1081 attgcgggtc tgatgactga ggacggaaaa cccgaaatcc cgacttcgct tgagggctga
     1141 accatttctg cggtccgcgc caccggtccg gagcacatgg acgggaagtt ggtcgtagat
     1201 caggccgtga tgggcgagag cgctccgatg cgaaaggcag cagctactcc cgcctgcgtg
     1261 aaccgcggcc tttcggcgcc cgatttcgtc aggccggcag ccgggcaggg tgaacacatc
     1321 gcgaaggacg ggtatcaaaa gcccggactc ctttctgcga cgaatggtct gcctggagac
     1381 tccgatttcc ctcaggtcat caccggtgat caccccgtga cctgacttca cgacctggcc
     1441 aagcttcctg ttcagttcgg ccgggctgac ccttcgtcgt gcatccatgg gaaaagcatg
     1501 aagcggaaaa gctcacacat ggctcgacag gagcaacaat tcagtaacag gaaaccgccg
     1561 aagcccgggc ggttgccgaa gcctgggcgg taccgaagcc tgggcggttg ccgaagcctg
     1621 ggcggttgcc gaagcctggg cggtaccgaa gcctgggcgg taccgaagcc tgggcggttg
     1681 ccgaagcctg ggcggttgcc gaagcctggg caatcgctga agcctgggca atcgccgaag
     1741 cctgaagccc ggagccgacg gctggtgcca cgagaagggt gccagtcccg gggcctggtt
     1801 cgtggcagaa ccagctctga ggcgaaaacc ccggggaccg gtgagagcgg ttcagccggg
     1861 gagggtcgcg gtgccggtca tgaccacgtg gacccggccg cttacccggg ggcggcccga
     1921 atccatgcgg gcctcgatca ggctggggcg gccgatctcg actccctggt ggatctcgac
     1981 ccggtcgacg tcgaggatgt cctgcaggta ggcgaccagc gggccggcgg ccgaaccggt
     2041 tgccgggtct tcgaaatcgc cgaggtcgag ggcgaaacag cgcgcgcgga ccgaacgggt
     2101 cgggacgtcg aagacgaagg gatacacgtt caccgcgaga tcaccgaatt cgaaactctc
     2161 gaactcggcc cggtcgggaa cggcatcggc cagcgcctcg acgttccgaa gcgggagcag
     2221 caccgtggga aaaccggtcg agacgacctg ggcggagtag tccgggtgaa ggcgcgactc
     2281 gtccagattg agcaggcgcg ccgcccggtc gcggggaacg gcctcgccga aactggccgg
     2341 ctcctgaagg accgaggccc gccagacccc cgacccggct gccgcctccc cgccctcggg
     2401 gcttggatcc gcagcacctg cccggctgac ctccacctgc tggaggccgg ccccggtctc
     2461 ctgggtgaag gtgatctcct cgaccccgcg gtcgagcgcg accgccacgg cggtaccgag
     2521 cgaagggtga ccggcgaagg gaacctcggt cgcgatcgtc cagatccggt tgcggtagtc
     2581 ggcgcccgac gaagccgctt ccgacgaagg cgactggacg aaggtggttt ccgagagacc
     2641 ggtctcgcgg gcgaaggcga gcatgatctc gtcgctgacc ccgtccgcgt cgaggaccac
     2701 ggcgagcccg ttgcccgcca gcggggattc ggcgaagacg tcgagccaga aaaaggggtg
     2761 gctgctcatc ggccaagcct agccggggca aaccggaccg ggtgcgtcaa tcctgttgtg
     2821 catcgagccg gcgaaagagc ccgccgtggt agatcagcgg ctcccccggt tcgtcctcgg
     2881 cctcgacttc gagtacctcg ccggtcacga tcacgtggtc tccgcccgga atcacttcgg
     2941 ccaggtcgca gaccacccgg gccaggcttc cctcgatgat cggcacctcc agatgctccc
     3001 gccagacgac cgacgaccat ttctcttcct ggtccgcctt cgtcgcgaac atctcggccg
     3061 cggcccgttg cgactcgccg aggaccgaga tcccgaaacg gccggcctcg cgaaccgcct
     3121 ccagagagcg ggaaccgagg tcgaggcaga ccagcatcat catcggctca agggagagcg
     3181 aacagaccgc ggaagcggtc gcgccggccg gtcccgaggc ctgaaatgcg gagaccacgg
     3241 tgactccggt cggcaacaaa cccaccgtct ggcgaaactc gtttcggtcc ggggctgcca
     3301 tcgtttcgcc gcagacaata tgatgcgctc gctgtgcgtg ccgtagccat cacaacactt
     3361 gccgtcgccg ccgccctcgg cctgtccgca tgcgggtttg gcgaagaagg aatcaccgtt
     3421 tcgaaggatt ccccggatta cgagggagcg caactcttcg ctgccaactg cgccggctgc
     3481 cacaccctga ccccggccgg ggctctcgga accggcaacc gcgggacccg gaaccagggt
     3541 ccgaacctcg accagcgggc cgtgacctac gaagacgccc ttcacgcaat ccagaacggt
     3601 ggattctccg gcgcgatcat gccgcagaac atcgtggtcg gggaagaagc cgagcaagtc
     3661 gcccggttcg tcgccgaata cgccgggtcc gacgccggtg aggcgggcgc cgaggcttac
     3721 tcggcagagg aagacgcttc ctccccgcag gatgagccga tcccgaccga cggccagtaa
     3781 ccggcggcgg gggccgtgct cgacctcaaa ctgatccgct ccgatcccga gcggatcaag
     3841 gctgccctgg cccggcgcgg cgccgccgac caggttgacg cactgctcac gctcgacgag
     3901 cgccggcgac agctcctgcc cgaaatcgaa ggcgcccgca gcgagcgcaa acaggaatcc
     3961 gagaagatcg gtgcgctgaa gaaggccggc gaggacgcct ccgcacaaat ggaggccgtg
     4021 cgccagctga aggccaggct cgaggagatg gaagccgagc tggaagccgt tgacggcaag
     4081 ctccgggagg ttgctgcgac gctgcccaac ctgcccgacc cgaccagccc cgacggatac
     4141 accgacgaag atgccgagct gatccgcgag gtcggcgaga ttccggattt cggcttcgag
     4201 cccagggacc acgtggaact tgccggcgac tcgatcgagc tggaagcggc ggcaagagtc
     4261 tccggtgcca ggttcgccta cctcaagggg gatctggtca tgctcgaatt cgcgctggtg
     4321 cgctgggcac tggagaaact tcgggccgaa ggccacgagc cggtggtgcc gccgatcctg
     4381 gttcgcgagg aagccctgtt cgggaccggt tttttgcccg gcgatcgcga tcagatctac
     4441 gaactggaga aggacgagct cttcctggcg ggcacctcgg aagtgccgct ggccggcctt
     4501 catgccgacc agatcctcga agccgatcag ctgccgcttc gctacgccgg attctcaagc
     4561 tgcttccgcc gcgaggcggg agccgcaggc cgggacaccc acggcatctt ccgggtccac
     4621 cagttcgaca aggtagagat gttctctttc gtcaagcccg aagacgcggg agccgaacac
     4681 gagcggatcc tggcgatcga ggaatcgatc ctgaccgaac tcgggctgcc ctaccgggtg
     4741 gtcaacgtgg cggtcggcga cctcggcggt tcggcggcca agaagtacga ctgcgaggcc
     4801 tggatcccga gccagggcag gtaccgcgag ctgacttcat gttcgaacac gaccgacttc
     4861 caggcccggc gaatcggcag ccgataccgg ccggaacccg gggcaccgcc cgaattcgtg
     4921 aacacgctga acggcacggc ggtcgcggtg ggaagaacga tcatcgccct gcttgagaac
     4981 catcagcagg ctgacggttc ggtccggctt ccggaatcgc tcgtgagcta cggagctccc
     5041 gcagtcatcg gcggcaattg acggatcaca gggtcctcat ctgtgatgac cactggatcg
     5101 tccgccaggc gattcgccag cgcatcgacg accttgaggg gttcaccgtg gtggaggagg
     5161 tcggg
//
LOCUS       DAOQFI010000028         5149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6513_length_5149_cov_2.701413, whole genome shotgun sequence.
ACCESSION   DAOQFI010000028 DAOQFI010000000
VERSION     DAOQFI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6513_length_5149_cov_2.701413"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..509)
                     /locus_tag="PLE93_00990"
     CDS             complement(51..509)
                     /locus_tag="PLE93_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH85650.1"
                     /translation="MSQNDEVIVRFYEAFAAGDADTMAGCYHERVHFHDPVFEDLNGP
                     EVMKMWRMLLGRSDDLEITLGEHQAEGDQGSGHWLAVYTFSQTGRVVHNEIDATFRFE
                     DGLIIDHRDSFDFWKWTRMALGVPGTLLGWSPIVQGKVRKQSKLLLAGAG"
     gene            complement(544..1605)
                     /locus_tag="PLE93_00995"
     CDS             complement(544..1605)
                     /locus_tag="PLE93_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNH85651.1"
                     /translation="MSTPLALMISLLLLAGNAFFVGAEFALISARRSVIELRADEGGW
                     AARITLHAMERVALMMAGAQLGITLCTLGLGALAEPAIAHALEHPFEGLGIPGSLVHP
                     AALVVALFLVGGFHVVLGEMVPKNIALAGPDRSALALGPPIAAVVKVLHPIIWVLNKT
                     ANGVLRLLGVTPSDEVTSAFTRDEVAGLVEESRREGMLDADETRLLTGALTFEERDAS
                     VVLLPIETMVTLPITVTPAEVEEAAGRTGFSRFPVTRDDEMIGYLHLKDALEVKEKHR
                     NRPIAESWVRPLPTVRLTDQLREVLEVMQSSGAHLARVVVRGARGRGKTVGVVALEDV
                     LEELIGEVRDAAQQIESES"
     gene            complement(1602..2957)
                     /locus_tag="PLE93_01000"
     CDS             complement(1602..2957)
                     /locus_tag="PLE93_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNH85652.1"
                     /translation="MIELLLVLVSVALVVACGAFVAAEFSFVTVDRSRVDREADEGDR
                     RARGVRQGLRTLSTQLSAAQVGITVTNLLIGYLAQPSVASLIDAPLESLGVSAGAVDG
                     VALTVAFILANAVTMIFGELVPKNLAIANAMGTAKAVQGFQRGFTRIIHPLVRVTNGI
                     ANWILRRVGIEPQEELASARSPEELTSLVRRSAEKGTLEEPTAELLEKSLEFGELRAD
                     DVMTPRVKVKTLAPDQPVIAVIEAARAGGFSRFPVISEGSEIVEGIVHVKHAVAVPHE
                     DRDDVPIRDVMADPVLVPSTIELDPLLAELRKVGLQVAIVVDEFGSFDGIVTLEDLIE
                     EIVGEVRDEHDRDEDPVREIAQGIWAVSGLMRPDEVEDQIGVRLPEDEDYETVAGLIG
                     FELGRVPGRGDSVRVEATNEEKEPLLADLYVLRMDGLRVDRVRLVVSKPQPEEDGEGE
                     K"
     gene            3142..4614
                     /locus_tag="PLE93_01005"
     CDS             3142..4614
                     /locus_tag="PLE93_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNH85653.1"
                     /translation="MASLLRRLLFAVLAATFLISSGASAAAPEPVVDGNQIVDSATGD
                     QFVPHGVNFPGYEYACKQGWNYGEEGTRPAEIASTVAGIASWNINTVRLPLNQDCWLG
                     DDGLPAGPGRTVAGYRQSVEDFVNALNAAGIVAIVDIHWSGPDGVVADGLRPMPDNRS
                     PAFWSSVATTFKDNPSVIFDLFNEPHSRWNADDTKVFGLSWDCWANGGCMAPAERDLA
                     KVSGLSWYRTVGMKKLTEVVRATGATQPILLSGTDYANDLRGWLANAPEDDQLIAGFH
                     NYLEQRCRTETCWDREVAPVAEHVPVLAAEFGQNDCGPPGHMNRFMDWADDHLIGYLA
                     WAWWSLPDEGCHNFALVSNLDGTPLAPAGTALNSHLASLPRVLPQPVKRVSPGLVIKR
                     AQWKRPTLRLRVAIKPNASAAITVQIRLARDSHARRAPRTNRRLITKKLRTGFGFVDL
                     HTAIPTGLKPVLVTVSYPGDKRLLPKTVKRKPGSVSKLTR"
     gene            4642..>5149
                     /locus_tag="PLE93_01010"
     CDS             4642..>5149
                     /locus_tag="PLE93_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH85654.1"
                     /translation="MTVRQTIEIHGQPVTYHKMGEGPPVLLVHGITSSSRTWKSVMPR
                     LAETHTVIAPDLLGHGRSAKPQGDYSLGAYASGMRDLLVALDVEKVNVVGHSLGGGVA
                     MQFAYQFPDRISRLVLVDTGGIGREVNPALRAAALPGAEFVLPLLFTPTLHDAGLKVR
                     NFFAGVGIH"
BASE COUNT          887 a         1779 c         1639 g          844 t
ORIGIN      
        1 ggcagagcag gttctcggtt accgcttcgt tggccccgac caaaccagcc tcagccggcg
       61 ccggcgagga gcagcttcga ctgcttgcgg accttgccct ggacgatcgg actccagccc
      121 agcaacgttc cgggcacacc gagtgccatc cgggtccact tccagaagtc gaagctgtcc
      181 cgatggtcga tgatcaggcc atcctcgaac cggaaggtgg cgtcgatctc gttgtggacc
      241 acccttccgg tctggctgaa ggtgtagacg gccaaccagt gtccggaacc ctggtcacct
      301 tctgcctgat gctcgccgag cgtgatctcc aggtcatccg agcgacccag cagcatccgc
      361 cacatcttca tcacttcggg cccgttcaga tcctcaaaga cggggtcgtg aaagtggact
      421 cgctcgtgat agcagccggc catcgtgtcc gcgtcaccag cggcgaaggc ttcatagaag
      481 cggacgatca cctcgtcgtt ctggctcatg cgccgatcct atccagcccg ggaggtgccg
      541 cggtcaggat tcggactcga tctgctgtgc cgcgtcccgg acctcgccga tcagctcttc
      601 gagcacgtcc tcgagggcga ccacaccgac cgtcttgccg cggccacggg cgccccgcac
      661 cacgacccgg gcgaggtgcg cgccggagct ctgcatcact tcgagcactt cgcgcagctg
      721 atcggtgagc cgcacggtcg gcagggggcg aacccaggat tcggcgatcg gccggttgcg
      781 gtgcttctcc ttgacctcaa gggcatcctt gaggtggagg taaccgatca tctcgtcgtc
      841 gcgggtgacc gggaagcggg agaagccggt ccggccggcc gcctcctcga cctcggccgg
      901 cgtgacggtg atcggcaggg tcaccatggt ttcgatcggg agcagaacca cgctggcatc
      961 ccgttcttca aaggtcaggg ccccggtcag cagccgggtt tcgtcggcgt cgagcattcc
     1021 ttcacgccgt gactcctcga ccagcccggc gacttcatcc cgggtgaagg cgctggtcac
     1081 ctcatcggaa ggagtcacgc ccagcagccg caggaccccg ttcgcggtct tgttgagcac
     1141 ccagatgatc ggatgaagca ccttgaccac cgcggcgatc ggcgggccga gcgccaacgc
     1201 ggaacggtcc ggcccggcca gggcgatgtt cttcgggacc atctcgccga gcaccacatg
     1261 gaagccgccg accagaaaca gggcgaccac cagggccgcc ggatggacca gtgacccggg
     1321 gatgcccagg ccctcgaagg ggtgctcgag cgcgtgcgcg atcgccggct cggccagggc
     1381 gcccagcccg agcgtgcaga gggtgatgcc gagctgggcg ccggccatca tcagggctac
     1441 ccgttccatc gcgtgaaggg tgatccgcgc cgcccagcca ccttcgtcgg cgcggagctc
     1501 gatcaccgac cggcgggcgg agatcagcgc gaactcggcc ccgacgaaga aggcattgcc
     1561 ggccagcagc aagagagaaa tcatcagggc cagcggggtg ctcacttctc gccctcccca
     1621 tcctcttccg gctgaggctt cgagaccacg agccgcacac ggtcgaccct gagcccgtcc
     1681 atccgcagca cgtagaggtc ggccagaagc ggctccttct cctcgttggt cgcttcgacc
     1741 ctgaccgagt cgccgcggcc gggcacccgg ccgagttcga agccgatcag cccggcgacg
     1801 gtttcgtaat cctcgtcttc agggagcctc accccgatct ggtcttcgac ctcgtccggc
     1861 cgcatcagcc cggaaaccgc ccagattccc tgcgcgattt cacgcaccgg atcctcgtcc
     1921 cggtcatgct cgtcacgaac ctcgccgacg atctcctcga tcaggtcttc aagcgtgacg
     1981 atgccgtcga agctgccgaa ctcgtcgacc acgatcgcga cctgcaggcc gaccttgcgc
     2041 agttcggcca gcagcgggtc aagctcgatt gtcgagggga ccaggaccgg atcggccatc
     2101 acgtcgcgga tcggcacatc gtcgcggtct tcatgaggga ccgcgaccgc gtgcttgaca
     2161 tggacgattc cctcgacgat ctcggatcct tcgctgatca ccgggaaacg ggaaaagcca
     2221 ccggcccggg ccgcctcgat taccgcgatc accggttgat ccggagccag ggtcttgacc
     2281 ttgacccgcg gagtcatcac gtcgtctgcc ctgagctcgc cgaactcgag tgacttctcg
     2341 agcaactcgg cggtcggctc ctccagcgtc cccttttcag ccgagcggcg gaccagcgag
     2401 gtcagctcct ccggtgaccg ggccgaggcc agttcttcct ggggctcgat cccaacccgg
     2461 cggaggatcc agttggcgat gccgttggtc accctgacca gcgggtggat gatccgcgtg
     2521 aatccgcgct ggaaaccctg gaccgccttg gccgtcccca tcgcgttggc gatcgcgagg
     2581 ttcttgggca ccagctcgcc gaagatcatg gtgacggcgt tggcgaggat gaaggcgacc
     2641 gtcaaagcga caccgtcaac ggcgccggca gaaaccccga gcgactccag cggggcgtcg
     2701 atcagcgaag cgaccgaggg ctgggcgagg taaccgatga gcaggttggt gaccgtgatc
     2761 ccgacctggg cggccgaaag ctgggttgaa agggtccgca gcccctgccg gactccgcgg
     2821 gcccggcggt caccttcatc ggcctcgcgg tcaacccggg accggtccac ggtgacgaag
     2881 gagaattcgg cagcgacgaa ggctccgcag gccacgacga gggcaacgga aacgagtacg
     2941 agcagcagct cgatcatcga ggcagcgctc ccggcacccg gctcaggaag cgcctgggac
     3001 tagaaccggg gtcgtcgtcc atccaggttt ccgcgagact tcatccggtt cgaagattac
     3061 ccctcacggg cagatgcatc ggtccgatac ccgctccgat cccgctgatc gcgggtttac
     3121 taccccagat accctttgtc agtggcatcc ctcctcagac gcctgctttt tgccgtgctc
     3181 gcggccacat tcctgatctc gtcgggggcg tcggcggctg cgccggaacc ggtggttgac
     3241 ggcaaccaga tcgtcgattc ggcgaccggg gatcagttcg tcccgcacgg ggtcaatttt
     3301 cccggctacg agtacgcctg caagcagggc tggaactacg gggaggaagg cacccgcccg
     3361 gccgagatcg cgtcgaccgt ggccgggatc gcatcttgga acatcaacac cgtccggctc
     3421 ccgctgaacc aggactgctg gctcggcgat gacgggctgc ccgccgggcc gggccgcacc
     3481 gtggccggat accgccagtc ggtcgaggat ttcgtcaacg ccctcaacgc ggccggcatc
     3541 gtcgcgatcg tcgacatcca ctggtccggg ccggacggcg tcgtggccga cggcctgcgc
     3601 ccgatgcccg acaaccgctc gcccgccttc tggagttcgg tcgcaacgac tttcaaggac
     3661 aacccgtcgg tcatcttcga cctcttcaac gagccccatt cgcgctggaa cgccgatgac
     3721 accaaggtct tcggcctgag ctgggactgc tgggcgaacg gcggctgcat ggctccggcc
     3781 gagcgcgacc tggccaaggt cagcgggctt tcctggtacc gcacggtcgg catgaagaag
     3841 ctgaccgagg tggtcagggc gaccggagcg acccagccga tcctgctttc cgggaccgat
     3901 tacgcgaatg acctgcgtgg ctggctggcc aacgcgccgg aagatgatca gctgatcgcc
     3961 ggtttccaca actatctcga gcagcgctgc cggaccgaga cctgctggga ccgcgaagtt
     4021 gctcccgtcg ccgagcacgt tccggtcctg gccgccgagt tcggtcagaa cgattgcgga
     4081 ccgcccggcc acatgaaccg cttcatggac tgggcagacg accacctgat cggatacctg
     4141 gcctgggcct ggtggtcgct tcccgacgag ggttgccaca acttcgccct ggtctccaac
     4201 ctggacggca ccccgctcgc cccggcgggg actgcgctga acagccatct cgccagcctt
     4261 ccacgggtgc tgccgcaacc ggtcaagcgg gtctcccccg ggctggtgat caagcgggcg
     4321 cagtggaagc gacccaccct gcggctccgg gtcgcgatca agccgaatgc ctcggcggcg
     4381 atcacggtgc aaatccggct ggcccgcgat tcgcacgcac gccgcgcgcc ccgcacgaac
     4441 cggcgattga tcaccaaaaa gctgaggacc gggttcggct tcgtcgacct tcacaccgcg
     4501 attccgaccg ggctcaaacc ggtcctggtc accgtttcct accccggtga caagcggctg
     4561 ctgccaaaga cggtgaagcg caaacccggt tctgtgtcca agctcacccg ttagaaagcc
     4621 gaaaagcggg aaaataggga catgacggta cgccagacga tcgagatcca cggccagccg
     4681 gtcacctacc acaagatggg cgaaggcccg ccggtcctgc tggtccacgg aatcacctcc
     4741 agttcgcgaa cctggaaatc ggtgatgccg cggctggccg agacgcacac ggtgatcgcg
     4801 ccggacctgc tcgggcacgg ccgctcggcc aagccccagg gtgactactc gctcggcgcc
     4861 tacgccagcg ggatgcgtga cctgctggtc gcgcttgatg tggagaaggt caacgtggtc
     4921 ggccattcac tcggcggcgg cgtcgccatg cagttcgcct accagttccc cgatcgcata
     4981 tcgcggctgg tcctggttga caccggcggg atcggccggg aggtcaatcc ggccctcagg
     5041 gccgccgccc tgcccggagc cgagttcgtc ctgccactgc tcttcacgcc caccctgcac
     5101 gatgccgggc tcaaggtgag gaacttcttc gccggggttg gcattcacg
//
LOCUS       DAOQFI010000029         5089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6641_length_5089_cov_3.836114, whole genome shotgun sequence.
ACCESSION   DAOQFI010000029 DAOQFI010000000
VERSION     DAOQFI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6641_length_5089_cov_3.836114"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PLE93_01015"
     CDS             <1..1414
                     /locus_tag="PLE93_01015"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNH85655.1"
                     /translation="DRVIIAPGTQTSTGSFKAPGNDPLEIIGSGRDKTFLTSSATGNT
                     YVLDLTSGSNRAVTVKNLAVVVPASFSTGAAISESADRFESVDLISRNDGSSGVFNPT
                     GGSSFSDVRAFGQNGAGFGGVFYQQSQCGTGPVSFERIHITDASSGLIWNCPEANPVT
                     VDRARFTGVGAAISVSDGALVSVTNTLVESGEGAPIRAFSATNKVTTLNLDHLTMVAT
                     GDPNQPAIRAQVNGVASPTKDIEISIGNSIISGFADSWSLEAPVDSVKGNVSLDASFL
                     ASDSDGLTAGESFVTGANNLALGGEAIFAAADDFHLATGSVVIDAGDPAATLPDHDLD
                     GEPRPVDGNADLVPVGDLGAYEYQPTCEEDPAFCPVDATAPRISKVSFTSRPGRGSSL
                     RLKISEAATVKAVFTPLSKRGRPKRGKVKLSRKARAGSLTIKLGKRKLKPGRYRLTLQ
                     AADKAGNKSKMITRKVRVKG"
     gene            1423..3003
                     /locus_tag="PLE93_01020"
     CDS             1423..3003
                     /locus_tag="PLE93_01020"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNH85656.1"
                     /translation="MRPVRFGVAAGTVMLALLVFSAPASATTFCVPGFFDACPDNGTN
                     QARTDLGDAMTDDGSDGIPDKVMIDSGTITSTGSIVAGGIDPLEVTGAGRDKTFLTSS
                     STGNISVLRINNRKGTKMSDLTIVVPASFPDEFGNGSAAQIGDSELTRVDVEVRNSDS
                     SAFASMVGSNLIRDSHVYAAEGSKVRWAFVTGGAPGKSTIIGARVEDPVYGFVADNPD
                     TSIEVKASRVIGPTAYGAWAGSGGQISLENTLIATYGGGALTADPENGESDPTIVTAV
                     SSTMVNTGTSTSPGIQVDIPNSITAGDAFVTVTDSIIRGYGATWDLFVPIGPGFAAAN
                     LNVVYSNFPPVAAPGSAGNVNVGSPTNINQDPLFVSPQDFRLKPGSPSVDAGNPASTL
                     TTDIDGSPRPRDGDGNRTSINDQGAFEFQPTCATAPALCPTVSKVKFRFRAGKGGSLR
                     FLLSLAAKVRAVFTPVPAHRKPKRKVVKITRKLKAGQVKIKLGKHRLMPDRYKLKITA
                     YDKTGHSAKPLIKKVTVK"
     gene            complement(3030..3446)
                     /locus_tag="PLE93_01025"
     CDS             complement(3030..3446)
                     /locus_tag="PLE93_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH85657.1"
                     /translation="MTPANLIVDASIAVKWFRPREIEPERELAEILIAEASIRITSLC
                     LYEVGNVLATKTRDSDEQIGRKLEALVHYCGPPLELTVPDFRLAGNLARRHKLTFYDA
                     SYVAIAERLGRKVVSADQDLIGPGLAVDLATAVGAG"
     gene            complement(3446..3682)
                     /locus_tag="PLE93_01030"
     CDS             complement(3446..3682)
                     /locus_tag="PLE93_01030"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNH85658.1"
                     /translation="MAKVMVSIPDELLAQIDAEARRQGTTRSGLLQRAARVEVGVRQV
                     SRDEVRAQLDGLSSDWIDPGEDITEAIRRDRRNH"
     gene            3855..4157
                     /locus_tag="PLE93_01035"
     CDS             3855..4157
                     /locus_tag="PLE93_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85659.1"
                     /translation="MLCALTVRKVKPGTAEEFIENFRPPDDEYPGGWKRFFAIRNLAD
                     ENEVITFGLFDGDLDQLNASQAEDNKYEDRINAVAPFIESTGTSGVFEVLVDQSID"
     gene            4574..>5089
                     /locus_tag="PLE93_01040"
     CDS             4574..>5089
                     /locus_tag="PLE93_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85660.1"
                     /translation="MKLKTGVAALAVSMLLFTADAGAAVSHQVTGPEAKASRGFWTAA
                     RVAAALKRDPDSALNAASSPDRHPVATHHRPVTVYRPEVGKILAWDRNIGEFSCSGSI
                     IDTRSLRLVLTAGHCVYADGVWARRHIFIPNFKNGRRPFGAYRVKATWVSNAWFNNSF
                     GTRGMNFDIGLL"
BASE COUNT          942 a         1691 c         1576 g          880 t
ORIGIN      
        1 cgacagggtg atcatcgctc ccggcacgca gacttccacc ggcagcttca aggcgcccgg
       61 caacgatccg ctggagatca tcggctcggg ccgcgacaag accttcctga ccagcagcgc
      121 aaccggcaac acctacgtgc tcgacctgac ttccggcagc aaccgggcgg tcacggtgaa
      181 gaacctggcc gtcgtggttc cggccagctt ctcgaccggc gcggcgatct cggagtccgc
      241 cgaccggttt gaatcggttg acctgatcag caggaacgac ggttcctccg gggtcttcaa
      301 cccgaccggc ggcagcagct tctccgacgt ccgcgccttt ggccaaaacg gggccggctt
      361 tggcggggtc ttctaccagc aaagtcaatg cggcaccggc ccggtttcct tcgagcggat
      421 ccacatcacc gacgcttcgt cgggcctgat ctggaactgc cccgaggcga atccggtcac
      481 ggttgaccgg gcccggttca ccggggtcgg agccgcgatc agcgtttccg acggcgccct
      541 cgtttcggtc accaacaccc tggtggaaag tggcgaggga gcgccgattc gcgccttcag
      601 cgccaccaac aaggtgacca cgctcaacct cgaccatctc accatggtcg ctaccggcga
      661 ccccaatcag ccggcgatcc gcgcccaggt gaacggtgtc gccagtccga ccaaggacat
      721 cgagatcagc atcggcaact cgatcatcag cggcttcgcc gactcctggt cactggaagc
      781 accggttgat tccgtgaagg gcaatgtgag cctggacgcc agtttcctgg cctccgattc
      841 ggacgggctt accgccggcg agtccttcgt gaccggggcg aacaacctcg ctctcggcgg
      901 cgaggcgatc ttcgcggcag ccgacgattt ccacctggcg accggttccg tcgtgatcga
      961 cgccggcgac cctgcggcca ccctgccgga tcacgacctc gacggcgaac cccggccggt
     1021 tgacggcaac gccgacctgg tcccggtcgg tgacctcggg gcctacgagt accagccgac
     1081 ctgcgaagag gacccggcat tctgcccggt cgatgcgacc gccccgagga tcagcaaagt
     1141 gagctttacc tccaggccgg gccgcggcag ttcgcttcgg ctcaagatct ccgaggcggc
     1201 cacggtcaag gccgtgttca cgccgctttc gaaacggggc aggcctaagc gcggaaaggt
     1261 caagctcagc cgcaaggcca gggcgggctc cttgacgatc aagctcggga agaggaaact
     1321 caagcccggc cggtacaggc tgacgcttca ggcagccgac aaggccggca acaaatcgaa
     1381 aatgatcacc cggaaggtcc gggtcaaggg ataggggcag acatgaggcc agtcaggttt
     1441 ggagtcgccg cgggaaccgt gatgctcgcg ctgctcgttt tctccgctcc ggcttcggcc
     1501 acgaccttct gcgtgccggg ctttttcgat gcctgtcccg acaacggcac caaccaggcc
     1561 cggacggacc tgggcgatgc gatgaccgat gacggcagcg atggaatccc ggacaaggtg
     1621 atgatcgact cggggacgat cacctccacc ggctcgatcg tcgccggcgg catcgatccg
     1681 cttgaggtga ccggggcggg cagggacaag accttcctca cctcgagctc gaccgggaac
     1741 atcagcgtgt tgcggatcaa caaccgcaag ggcacgaaga tgtccgatct gaccatcgtc
     1801 gtaccggctt cgttccctga cgaatttgga aacggttcgg ccgcccagat cggggattcg
     1861 gagctgacca gggttgacgt ggaggtcagg aactccgatt cgagcgcctt cgcctcgatg
     1921 gtcgggagca acctgatcag ggattcacat gtgtacgcgg ccgaagggtc gaaggtgcga
     1981 tgggcattcg tcaccggcgg ggcaccgggc aagtcaacga tcatcggagc cagggttgaa
     2041 gacccggtct acgggttcgt agccgacaac cccgatacct cgatcgaggt gaaggcaagc
     2101 cgggtgatcg gcccgaccgc ttatggcgcc tgggccgggt cgggagggca aatcagtctc
     2161 gagaacaccc tgatcgcgac ctacggggga ggcgctttga cggcggaccc ggagaacggc
     2221 gaatccgacc cgaccatcgt gaccgcggtc tcgtcgacga tggtcaacac cggcacctcg
     2281 acatctcccg ggattcaagt cgacattccg aattcgatta ccgccggtga cgccttcgtg
     2341 acagtcaccg actcgatcat ccgtggctac ggggcgacct gggatctgtt cgtcccgatc
     2401 gggccgggct ttgccgcggc gaacctcaac gtcgtctatt caaacttccc cccggtcgcc
     2461 gccccgggct cggccggaaa cgtaaacgtc gggagcccga ccaacatcaa ccaggatccg
     2521 cttttcgtca gcccccagga ctttcgcctg aagccgggct caccttcggt tgatgcgggc
     2581 aatccggcct cgaccctgac caccgacatc gacggcagcc cccgcccccg ggacggtgac
     2641 ggcaaccgga cctcgatcaa cgatcagggt gctttcgagt ttcaacccac ctgcgccacc
     2701 gccccggcac tttgcccgac cgtctcgaag gtgaagttca ggttcagggc cggcaaaggc
     2761 ggttcgctcc gcttcctcct ctccctggcg gccaaggtga gggcggtctt cacgccggtc
     2821 cccgcgcacc ggaagccgaa gcgcaaggtg gtgaagatca cccgcaagct caaggccggc
     2881 caggtgaaga tcaagctcgg caagcaccgg ctcatgccgg accgctacaa gctgaagatc
     2941 accgcctacg acaaaaccgg ccacagcgcc aagcccctga tcaagaaggt caccgtcaag
     3001 taatcccgcg cccgggccgg gagcgttctt cagccagccc cgaccgcagt agccaggtca
     3061 accgccagcc ctggcccgat caggtcctgg tcggcagaaa ccaccttccg tcccagccgt
     3121 tcggcgatcg ccacatacga agcgtcatag aaggtgagct tgtggcgtcg ggccaggttg
     3181 ccggcgaggc ggaagtcggg gacagtcagc tcgaggggag ggccgcagta gtggacgagc
     3241 gcctccagtt tgcggccgat ctgttcgtcg ctgtcacgcg tcttggtcgc gagcacgttc
     3301 ccgacctcgt acaggcatag cgaggtgatc ctgatcgacg cctcggcgat gaggatctcg
     3361 gccagctccc gttccggttc gatctccctg ggccggaacc acttgaccgc gatgcttgca
     3421 tcgacgatca ggtttgccgg agtcatcaat ggttccggcg atcccgccgg attgcttcgg
     3481 ttatgtcctc cccggggtcg atccagtccg aggaaagccc gtccaactgg gctcgaactt
     3541 cgtcacggct cacttgccgg actccgacct cgaccctcgc agctcgctgg agcagcccgc
     3601 ttctcgtcgt gccctggcgc cttgcttcgg cgtcgatctg ggcgagcaat tcatcgggta
     3661 tggagaccat aaccttcgcc atccttccga gtatatcccg ttggcaatac ccacttctgt
     3721 ctttcgaaca tgccacaccc gcaagcggaa tcggcttcag ggccacgaat cgtcacggtt
     3781 ctgtcacaga gattttctcg ttctgttgca cagttgcggt taggctcggt gcggaggcag
     3841 agaaaggagc agtcatgctg tgtgcattga cggtccgcaa ggtgaagccg ggcactgccg
     3901 aggagttcat cgagaacttc cgcccgcccg acgacgaata ccccggcggc tggaagcggt
     3961 tctttgcgat ccgcaacctg gccgacgaga acgaggtaat caccttcggg ctcttcgacg
     4021 gtgacctcga ccagctgaat gccagtcagg ccgaggacaa caagtacgaa gacaggatca
     4081 acgcggtggc cccgttcatc gaatcgaccg gaaccagcgg cgtattcgag gttctggtcg
     4141 atcagtcaat cgactgaatc gcggcggcat ccccggcggc ccggtcgggc gccccgcgag
     4201 gggcgcgccg gccagcttca gagatgccgc cgcccgcggc gcccaacatc tgggtcggac
     4261 gccggccagt cgaagctcag tcggagttca tccagatcaa ccagtggacc gggctcacgg
     4321 ttccggtagg tggccagctc gcttcgcacg gccgactgga acatttcgtc ccggtcaacc
     4381 ccgcggatcg cggcttgcct ctccattcct gtgagcagtc cgtccgggat ctgtccctcc
     4441 gtccttgcca ttggccgaac atagcgccgg tccttcggcc tgtgtgagat atgtcaaccc
     4501 cgggtcgatg aaaaacccgg catttgcgga attcctttgt cagatcaggg agaatggaag
     4561 cgacatgggg ggcatgaaac tgaaaacggg agtcgcagct ctcgctgtct cgatgcttct
     4621 gttcaccgcc gatgccggcg cagcggtcag ccaccaggtg accgggccgg aggcgaaggc
     4681 atcccggggt ttctggacag cggcccgggt ggctgccgcg ctcaagcgtg acccggactc
     4741 cgctctgaac gccgcctcct cgcctgaccg gcacccggtc gcaacccatc accgtccggt
     4801 cacggtgtac cggcccgagg tcggcaagat cctcgcctgg gatcgaaaca tcggcgagtt
     4861 cagctgcagc ggcagtatca tcgacacccg cagcctgcgg ctcgtcctca ctgcgggcca
     4921 ttgcgtctat gccgacgggg tctgggcgcg gaggcacatc ttcatcccga acttcaagaa
     4981 cggccgccgt ccgttcgggg cctaccgggt caaggccacc tgggtgtcca atgcctggtt
     5041 caacaattcc ttcgggaccc ggggcatgaa tttcgacatc ggactgctg
//
LOCUS       DAOQFI010000030         5075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6668_length_5075_cov_3.823307, whole genome shotgun sequence.
ACCESSION   DAOQFI010000030 DAOQFI010000000
VERSION     DAOQFI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6668_length_5075_cov_3.823307"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /locus_tag="PLE93_01045"
     CDS             <1..2251
                     /locus_tag="PLE93_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNH85661.1"
                     /translation="DADSDKIRNEVIRMLSGPGGRRPGQSGSGSSSGSSQGEGSGKKS
                     SKLLDQFGRNLTKLAEDGKLDPVVGRETEIERIMQILSRRTKNNPVLLGEPGVGKTAV
                     VEGLAARIIKGEVPEMLRGKQIYTLDLAALVAGSKYRGEFEERLKKVMKEIASRGDIV
                     LFIDEIHNLVGAGAAEGAIDAASILKPALARGEIQTIGATTLDEYRKYLERDSALERR
                     FQQIRVEQPTPEQTVKILEGLRERYEQHHKVQISDDALEAAAELADRYISDRFLPDKA
                     IDLIDEAASRMRIKSMTAPPVYRDLEEEIETARREKEAAIEAQEFEKAANLRDTERQL
                     TTRKRQLEEEWAEGEEGERPVLGEEEIADIVSMWTGIPVFKLTEAETKKLIQMESELH
                     KRVIGQEAAIEAVSKAIRRSRAGIKDPKRPAGSFVFLGPSGVGKTELARTLAEFLFGD
                     EESMVRIDMSEYMEKHAVSRLVGSPPGYVGYDEGGQLTEAVRRKPYSVLLLDEIEKAH
                     PDVFNILLQILEDGRLTDSQGRTVDFRNAIIIMTSNIGASEIARNQGIGFTVSDDTGI
                     TYDDMKGRIMSELRKVFRPEFLNRIDEVIVFHKLSREEVREIIELMLARVRNQVAEHE
                     LMLELDNGAKDFLADKGWDPSMGARPLRRAIQRYIEDPLADEVLKAGGGDSVAGSLVT
                     VTRDESGDDDDKPLKLKITKPRKPRKKKEDGQEKVGVGAGSTDSPSSEPPAVKPDVAD
                     GPAAESAAD"
     gene            2391..4448
                     /locus_tag="PLE93_01050"
     CDS             2391..4448
                     /locus_tag="PLE93_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNH85662.1"
                     /translation="MGALATFAFACLLMASPSLASAGWTSPATVSGPGTSVSQTTVAG
                     GSDGTSWVVWKRDVGGFNLIQGTRVQIDGTQGPIVTLSPVGQNANNPVIASRADGSAV
                     VGWTNESGTSDFIQSVSIAADGTVGAVTDRSVNGPAGQDASDLAIAVGADGTAGLAWL
                     RFDGTEPVVQAVRVASDGSSGTVHNLSAASAAPGPPDVGAAPPTTAGDPYFYRVMWPQ
                     GSAATSQVANADVMANDTVTTTVLLYTNASGADCADPFDIQIAYGSDGAMNAFWVCYK
                     KTIDFNTFNTFYNWSVQWLRTAKGVPLSTGGAASATNASPTIYLSPYPISGVVAAESF
                     TGQPAIAWIHDLDGGGQRVETWRIMINVLNGVPLPVGQGWANTTTEATAVGSPAIAVN
                     SNNTGIAGGVELGALPGQNTTNFTRFSNSSYDPVTPSGGFTYSEDPGYVLTDGGKTLA
                     AFTGITGPVGSAMVMTFEDPGISVDPDSFNFGHADIGTKRSVSISIRSTGQTTNTVTG
                     ITLGGANADRYAMSGADDCIREMTPNTNCRFTVTFTPGSTASQTAQISVTSDGGNEVA
                     NLTGTGLNRTRNSITAKPGHAARRKGKVVRIRVKASNLGGVSSNNTRICVNLRKRALK
                     LAGNRCRNLGTLGVGATRNLNYRIRVTWRAQRGVKLPVTFVLRSNNTVVRQAVVQLRR
                     KGR"
     gene            complement(4510..>5075)
                     /locus_tag="PLE93_01055"
     CDS             complement(4510..>5075)
                     /locus_tag="PLE93_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85663.1"
                     /translation="PRPINYRDNHRVPGEKLLRAWRKRSDMPYKRFVDGRFKGTTDEI
                     IQWASLKWGIDTDTMRAAAVVESWWDMDVRGDNGDSFGLYQVRRPYHCWGKCKIARYF
                     TAFNADYYGAIIRSYYDGKQTWLNTVSGNGKKYRAGDLEGSMGAWYSGRWWGPPERPI
                     EPYLEKIARQKADRTWRHADFIAYRQP"
BASE COUNT          934 a         1709 c         1632 g          800 t
ORIGIN      
        1 cgacgccgac tcggacaaga tccgcaacga ggtcatccgc atgctttccg gccccggcgg
       61 ccgccggccc ggccagagtg gcagcggttc ctcctccggc tcaagccagg gcgagggttc
      121 cggcaagaag tcctcgaagc tgctcgacca gttcggccgc aacctgacca agctggccga
      181 ggacggcaag ctggatccgg tcgtcggtcg cgagaccgag atcgagcgga tcatgcagat
      241 cctttcccgc cgcaccaaga acaacccggt cctgctgggt gagcccgggg tcggcaagac
      301 cgccgtggtc gagggtctgg ccgcccgcat catcaagggc gaggtcccgg agatgctgcg
      361 cggcaagcag atctacaccc tggacctggc cgcactggtc gccggctcca agtaccgcgg
      421 cgagttcgag gaacggctca agaaagtgat gaaggaaatc gcctcccggg gtgacatcgt
      481 cctcttcatc gacgagatcc acaatctggt cggtgccggc gccgccgagg gcgccattga
      541 cgcggcctcg atcctcaagc cggccctggc ccgtggcgag atccagacga tcggcgcgac
      601 caccctcgac gagtaccgca agtacctcga acgcgactct gccctggagc gccgcttcca
      661 gcagatccgg gtcgaacagc cgaccccgga acagaccgtg aagatcctcg aaggcctgcg
      721 cgagcgctac gagcagcacc acaaggtcca gatctccgat gacgccctgg aagccgcggc
      781 cgagctggcc gaccgttaca tctccgaccg tttcctgccg gacaaggcga tcgacctgat
      841 tgacgaagcc gcctcgcgga tgcggatcaa gtcgatgacc gctccaccgg tctaccgcga
      901 tctcgaagaa gagatcgaga ccgcccggcg cgagaaggaa gccgcgatcg aggcccagga
      961 attcgagaag gccgccaacc tgcgtgacac cgaacgccag ctcaccaccc gcaagcgcca
     1021 gctcgaagag gagtgggccg aaggcgagga gggcgagcgc ccggtgctcg gcgaggaaga
     1081 gatcgccgac atcgtctcga tgtggaccgg catcccggtc ttcaagctga ccgaggccga
     1141 gaccaagaag ctgatccaga tggagagcga gctgcacaag cgcgtgatcg gtcaggaagc
     1201 cgcgatcgaa gccgtctcga aggcgatccg tcgctcgcgg gccggcatca aggacccgaa
     1261 gcgtccggcc ggctcattcg tcttcctcgg cccatcgggc gtcggcaaga cggagctcgc
     1321 gaggaccctg gccgagttcc tcttcggcga cgaggaatca atggtccgga tcgacatgtc
     1381 cgagtacatg gagaagcatg cggtcagccg tctggttggc tcgccccccg gctacgtcgg
     1441 ctacgacgag ggcggccagc tgaccgaggc cgtgcgccgc aagccgtact cggtgctgct
     1501 gctcgacgag atcgagaagg cccacccgga tgtcttcaac atcctgctcc agatcctgga
     1561 agacggccgc ctgaccgatt cgcagggccg caccgtggac ttccgcaacg cgatcatcat
     1621 catgacctcg aacatcgggg cttccgagat cgcccgcaac cagggcatcg gcttcacggt
     1681 ctccgacgac accggcatca cctacgacga catgaagggt cgcatcatga gcgagctccg
     1741 gaaggtcttc cggccggagt tcctcaaccg gatcgacgaa gtgatcgtct tccacaagct
     1801 gagtcgcgag gaagtgcgcg agatcatcga gctgatgctg gcaagggtca ggaaccaggt
     1861 ggccgagcac gagctgatgc tcgagctcga caacggcgcc aaggacttcc tcgccgacaa
     1921 gggctgggac ccgtcgatgg gcgcccgacc gctccgccgg gcgatccagc gctacatcga
     1981 ggatccgctg gccgacgagg tgctgaaggc gggcggcggc gattcggtgg ccgggtcact
     2041 ggtcacggta acccgcgacg agtcgggcga cgatgacgac aagccgctca agctgaagat
     2101 caccaagccg cggaagcctc gcaagaagaa ggaagacggt caggagaagg tcggtgtcgg
     2161 ggccggttcc accgattccc cctcgagcga gcctccggcg gtcaagcctg acgtggccga
     2221 cggtcccgcc gccgagtcgg ctgccgactg agcagcgtcg cttccgccgc ttctcacctc
     2281 accgaccgcg tcacttgatt gtttccggtg ttcctacagc tagactgacc cgacagcaac
     2341 tgggacggag ggttcgcttg cggtatggcc gtgcaaaaac cggatcgatt atgggcgcgc
     2401 tggcgacttt cgcctttgcc tgcctgctga tggcttcgcc ttcgctcgcg agcgccggct
     2461 ggacttcgcc ggccacggtt tccgggcccg ggaccagcgt cagccagacc acggtggccg
     2521 gtggcagcga cggcacttcc tgggtcgtct ggaagcgtga cgtgggcggt ttcaacctga
     2581 tccagggcac ccgagtccag atcgatggca cccagggtcc gatcgtcacc ctctctccgg
     2641 tcggccagaa cgccaacaat cccgttatcg ccagccgggc tgacggctcg gcggtggtcg
     2701 gatggaccaa cgagagcggc acttccgatt tcatccagtc ggtttcgatc gccgccgacg
     2761 gcaccgtcgg ggcggtcacc gaccggtcgg tcaacgggcc ggccggccag gatgccagcg
     2821 acctcgcgat cgccgtcggc gccgacggca ccgccggtct tgcctggctc cgctttgacg
     2881 gcaccgagcc ggtcgtccag gccgtcaggg tcgcgtccga cggcagttcc gggacggttc
     2941 acaacctgtc cgccgcctcg gctgcgcccg gtccgcccga tgtcggcgcc gcccctccga
     3001 ccacggcggg cgacccatac ttctaccggg tcatgtggcc ccagggctcg gcggcgacca
     3061 gccaggtcgc caacgccgac gtcatggcca atgacacggt caccaccacc gtcctcctgt
     3121 acacgaatgc gagcggggcg gactgtgccg atcccttcga cattcagatc gcctacggct
     3181 ccgacggagc catgaacgcc ttctgggtct gttacaagaa gacgatcgat ttcaacacct
     3241 tcaacacctt ctacaactgg tcggtccagt ggctgcgtac ggccaagggg gtgccactca
     3301 gcacgggagg ggcggcttca gccaccaacg cctcaccgac catctatctg agcccgtacc
     3361 cgatcagtgg cgtcgtcgcg gccgagtcct tcaccgggca gccggccatc gcctggatcc
     3421 acgaccttga cggcggcggc cagcgggtcg agacctggcg gatcatgatc aacgtgctga
     3481 acggggttcc gcttccggtc ggccagggct gggccaacac gaccaccgaa gcgaccgcgg
     3541 tcggctcccc ggcgatcgcc gtcaactcga ataacaccgg catcgcgggt ggcgtcgagc
     3601 tgggagcgct gccggggcag aacaccacca atttcacccg gttctcgaac agttcctatg
     3661 acccggtgac gccctccggt ggcttcacct acagcgaaga tccgggttac gtactgaccg
     3721 acggcggcaa gacactggcc gccttcaccg gaatcaccgg tccggtcggg tcggccatgg
     3781 tgatgacctt cgaagacccc gggatcagcg tggatccgga cagcttcaac ttcggccatg
     3841 ccgacatcgg tacgaagcgg agcgtctcga tctcgatccg cagcacgggg cagaccacca
     3901 acacggtcac cgggatcacc ctgggcggag ccaacgccga tcgttacgcg atgagcggag
     3961 ccgacgactg catcagggaa atgaccccca acaccaattg ccgcttcacg gtgactttca
     4021 cgcccggttc gaccgcgagc cagaccgcac agatttcggt tacctcggac ggcggaaacg
     4081 aggtcgccaa cctgaccggc accggcctca accggacccg gaactcgatc acggcaaagc
     4141 ccggccacgc cgcccgacgc aagggcaagg tcgtccggat cagggtcaag gcctcgaacc
     4201 tgggcggggt atcgtcgaac aacacgagga tttgcgtgaa cctccgcaag cgggctctga
     4261 agctggccgg caaccgctgc cgcaacctgg gaacgctcgg ggtcggggcg accagaaatc
     4321 tcaattaccg gatccgggtg acctggcgcg cccagcgtgg cgtcaagctt cccgtcacct
     4381 tcgtcctgcg ctcgaacaac accgtggtgc ggcaggccgt ggttcagctt cgccgcaagg
     4441 gccgctaggc aaaaactccc tggcggattc ggaacctgcc gcgggtggca ggttcatttc
     4501 cggtaggccc tagggctgcc ggtaggcgat gaagtccgcg tggcgccagg tccggtcggc
     4561 cttctgccgg gcgatcttct cgaggtatgg ctcgatcggg cgttccggcg ggccccacca
     4621 gcgtcccgag taccaggcgc ccatgctgcc ctcgagatcc ccggcccggt acttcttgcc
     4681 gttgccggaa acggtgttca gccaggtctg cttgccgtcg tagtaggaac ggattatcgc
     4741 gccgtagtag tcggcgttga aggcggtgaa gtaccgggcg atcttgcatt tgccccagca
     4801 gtggtagggc cggcgcacct gatagaggcc gaatgaatct ccgttgtcgc cgcgcacgtc
     4861 catgtcccac caggattcga ccaccgcggc ggcgcgcatc gtgtcggtat caatgcccca
     4921 cttgagggag gcccactgga tgatctcgtc ggtcgtgccc ttgaagcggc cgtcgacgaa
     4981 acgcttgtac ggcatgtcgc tgcgcttgcg ccaggcgcgg agcagctttt cgcctggtac
     5041 ccggtgattg tcccggtagt tgatcgggcg cggct
//
LOCUS       DAOQFI010000031         5018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6786_length_5018_cov_3.537981, whole genome shotgun sequence.
ACCESSION   DAOQFI010000031 DAOQFI010000000
VERSION     DAOQFI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6786_length_5018_cov_3.537981"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLE93_01060"
     CDS             <1..688
                     /locus_tag="PLE93_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNH85664.1"
                     /translation="PSIGDFMEVYGPLLDEGREIVSLHLSGAISGTCEAARQAAVQLE
                     ADGRGGERITVVDSRTTAGGLAFCNLAAANAIKDGKDVAGVVAQIEACREEIDTLILV
                     DTLEYLRKGGRIGGAQAWIGGALKIKPLITLRETVEPVDKVRTLSRAMQRLKDHASEK
                     AASGQEITWTAQHIQAPELAAELAAHCREVFQCEGAFLDEMSTVLGCHAGPGLVAIGT
                     VPTDAVGSKF"
     gene            732..1259
                     /locus_tag="PLE93_01065"
     CDS             732..1259
                     /locus_tag="PLE93_01065"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNH85665.1"
                     /translation="MNLAVTAIGRDRPGIVAAITSALLEAGGNVDDSQMSILHGHFAV
                     MLIVSVPADASLESLEARLAEVGREFDLGGITVSPVEGLDRGAGPTHVLTVYGADRPG
                     IVHDTAATLASLGVNITDLRTRRTGDPDSPLYTLMVEVAVPEGTGPELDQALDGLRQS
                     KQLEARLAELESEVF"
     gene            1293..1814
                     /gene="def"
                     /locus_tag="PLE93_01070"
     CDS             1293..1814
                     /gene="def"
                     /locus_tag="PLE93_01070"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNH85666.1"
                     /translation="MVVREVLTYPDPILKSVCSPAQSGPGDREETGRVITDLLDTMNS
                     FDHCVGLAAPQIAESVRIAVVDITGHPRAKEPHGLMVLVNPRVVHRSEGTKVSREGCL
                     SLPDLTANVRRPRKATVEFLDGQRNLEMNQVQLAGFEARCVLHEIDHLDGILFLDRVA
                     SITDDLFRRQNYG"
     gene            complement(2289..2684)
                     /locus_tag="PLE93_01075"
     CDS             complement(2289..2684)
                     /locus_tag="PLE93_01075"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HNH85667.1"
                     /translation="MSDEKPETGASGSENPVEGVEKPFRPEGVGPEAPVDRTPNRHVA
                     PEGTPAPAAGAAGSPYSPPPPKRNSTQIRADIDAQRAELGKSVDQLRDRVTELTDWKS
                     QLRKHRKQIIVGAVATGFVVGGLMALRRR"
     gene            complement(2677..3051)
                     /locus_tag="PLE93_01080"
     CDS             complement(2677..3051)
                     /locus_tag="PLE93_01080"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNH85668.1"
                     /translation="MVFEVSERTSNLIREEIELAKTEVSEKVTTLARGSAVGIAAGVF
                     AFLALILLMHAFALGVASLFGWGAWAGYLLEGVIFIAIAGGAGYFAYSSFEKTGAPIP
                     TEAIEEAQKTKALLTPGEDSNE"
     gene            complement(3148..3282)
                     /locus_tag="PLE93_01085"
     CDS             complement(3148..3282)
                     /locus_tag="PLE93_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85669.1"
                     /translation="MDNDIVRRLTWSVMLTASSALASLIAARLAAVAYRRFFNEDPPE
                     "
     gene            complement(3434..4033)
                     /locus_tag="PLE93_01090"
     CDS             complement(3434..4033)
                     /locus_tag="PLE93_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH85670.1"
                     /translation="MAREAAPERRRQILDAAVRVFARQGFHSTRVADIADEADVAYGL
                     VYHYFSSKENVLNELFIQRWSLLIEAIDEADRSTLAHEEKLGAVAGFIIDSYRYDPDL
                     MKVIIVEVTRAANSFGQTHLEEINRAYELIGRIVADGQKQSIFREDIDPAFAAMVFYG
                     AIEQLLSGWIFGVIPGSDRDFDEARDLLVKAICEGLERR"
     gene            complement(4023..4865)
                     /locus_tag="PLE93_01095"
     CDS             complement(4023..4865)
                     /locus_tag="PLE93_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNH85671.1"
                     /translation="MRAAVVQINSGADRSRNLEVAGELVVAAAADGAELVVLPEKWAL
                     LGDGAALTAGAESLDGEAITAARGWAADLGIHLLAGSFTMLPDPGGVPPSALPTNTSV
                     LISPEGQIVCSYDKLHMFDVEAGGVEYRESDHEQAGDGLAVADAGDLRIGLTICYDLR
                     FPELFRALLDRGATAFTVPSAFTSATGRDHWETLLRARAIENQAFVLAANQVGTAAPS
                     YDSWGHSMIVGPWGEVLAEVKEGEGFACAELDLERQAEVRRKLPAVNHRRPEIFSEGV
                     AHGS"
BASE COUNT          848 a         1642 c         1701 g          827 t
ORIGIN      
        1 accctcgatc ggggatttca tggaggtcta cgggccgctt ctcgacgagg gcagggagat
       61 cgtctcgctg cacctctcgg gcgcgatctc cggaacctgc gaagccgccc ggcaggccgc
      121 ggtccagctc gaagccgacg gtcgcggcgg ggaacggatc accgtggtcg attcccgcac
      181 caccgccggc ggcctggcct tctgcaatct tgccgccgcg aacgcgatca aggacggcaa
      241 ggacgtggcc ggggtcgtcg cccagatcga ggcctgccgc gaggagatcg acacgctgat
      301 cctggttgac accctcgagt acctgagaaa gggcggccgg atcggcgggg cccaggcctg
      361 gatcggcggg gcgctcaaga tcaagccgct gatcaccctc cgcgagaccg tcgagccggt
      421 cgacaaggtg cgaaccctct cccgtgccat gcagcggctg aaggatcacg ccagcgagaa
      481 agccgcctcc gggcaggaaa tcacctggac cgcccaacac atccaggctc cggaactggc
      541 ggccgaactg gccgcccact gccgcgaggt ctttcagtgc gaaggagcat tcctcgacga
      601 gatgtcgacc gtgctgggat gtcacgccgg cccggggctg gtcgcgatcg gcacggtgcc
      661 caccgacgcg gtcggctcga agttctgatg actgcggcgc taccgcgggc ctggcgggcg
      721 aggctgcgcc cgtgaacctg gccgtcaccg cgatcggccg cgaccgtccc ggcatcgtcg
      781 ccgcaatcac cagcgccctg ctcgaagcgg gcggcaacgt ggacgactcg cagatgtcga
      841 tcctccacgg ccatttcgcc gtgatgctga tcgtctcggt tcccgcggat gcctcgctcg
      901 agtcgctgga ggcgaggctg gccgaggtcg gccgcgagtt cgacctgggc ggcatcacgg
      961 tcagtccggt cgaggggctg gaccgtggag cgggaccgac ccatgtactc acggtctacg
     1021 gggccgaccg tcccgggatc gtccatgaca cggcggccac gctggcttca ctcggcgtca
     1081 acatcaccga cttgcgaacc cggcgcacgg gcgatcccga ttcgcccctc tacacgctga
     1141 tggtcgaggt agcggtgccg gaaggaaccg gtccggaact cgaccaggcc ctcgacgggc
     1201 tgcgccagtc gaagcagctg gaagcccggc tcgccgaact cgagtccgag gtcttctaag
     1261 tggccggctc cgcggtctga ggcaggcaac cgttggtagt tcgcgaggtc ctcacctatc
     1321 ccgaccccat tctcaagtcg gtctgttccc ctgcccagtc gggaccgggt gaccgcgaag
     1381 aaaccgggcg ggtgatcacc gacctgctcg acacgatgaa ttccttcgat cactgcgtcg
     1441 gcctggccgc gccccagatc gcggagtcgg tccggatcgc cgtggtcgac atcaccggcc
     1501 atcccagggc gaaagaaccg cacgggctga tggtgctggt caacccccgg gtggttcacc
     1561 gctcggaagg aacgaaggtc agccgcgagg gctgtctctc gctgcccgac ctcacagcca
     1621 acgttcgccg cccccgaaag gccacggtcg agtttctcga cggccagagg aacctcgaga
     1681 tgaaccaggt ccagctggcc ggcttcgagg cccgctgcgt cctccacgag atcgaccacc
     1741 tcgacggaat cctcttcctc gaccgcgtcg cctcaatcac cgacgatctc ttccgccgcc
     1801 agaactacgg ctaacccgca accgacccac tgcgctgtcg aggaccggtt cggatggtcc
     1861 ggtgggtcgg ttttctggct gctacgacgg aaatccgacc cactgcggtg gaacagggca
     1921 ggtgaagggg gaagttgaag ggatcccggg tccgagctgg cttcacggcc aggtcgcgtc
     1981 cctatccagg ctggtgccgc aggtttgcaa cctctgaggt cggaaacctg cggcaccggc
     2041 cccgaatcac ggtcaggaat cgatcgagat gctgcttgtc ggcgctatat gccgacaaac
     2101 cgcatctcgc gaaatgtcaa caccaccaac cgtgagcggg tcgtgcccag gggcctccag
     2161 ccgttcgctg gcaaggagac aggccggaaa tgaccgcgat agtacgcggg tacatgagct
     2221 gtcgtttccg gccgacgacg aagctagcgg acggctggtg gttcctgggc gcggtgcccg
     2281 ggggctgtct accggcggcg gagggccatt agtccgccga ctacaaagcc ggtggcgacc
     2341 gcgccgacga tgatctgctt gcggtgcttg cggagctggc tcttccagtc ggtgagctcg
     2401 gtcacccggt cgcggagctg gtccacggac ttgccgagct cggccctctg ggcgtcgatg
     2461 tccgcccgga tctgggtcga gttccgcttc ggaggcggtg gcgagtaggg cgagcctgcc
     2521 gcgccggcgg ccggggccgg ggtgccctcg ggagcgacat ggcggttcgg tgtgcggtcg
     2581 accggggcct ccgggccgac gccctcggga cggaaaggct tctcgacacc ttcgaccgga
     2641 ttctccgatc cggatgcgcc cgtctcgggc ttttcgtcac tcattggaat cttctccggg
     2701 ggtcagcagg gccttggtct tctgggcttc ctcgatcgcc tcggtcggga tcggggcgcc
     2761 ggtcttctcg aaagagctgt aggcgaaata cccggcgccg ccggcgatcg cgatgaagat
     2821 cacgccttcc agcaggtagc cggcccaggc gccccagccg aagagcgagg cgacgccgag
     2881 cgcgaaggcg tgcatcagca ggatcagtgc gaggaaggcg aagactccgg cggcgatgcc
     2941 gaccgccgag ccgcgggcca gggtggtgac cttctcgctg acctcggtct tggcgagctc
     3001 gatctcttca cggatcaggt tcgaggtgcg ctcggaaacc tcgaagacca tttccccgac
     3061 ggtcttttcg gatgccgggc gattcggatc cgggcggccg ggtggtcgct ggtagggcgg
     3121 cggctggtag tcctctggag ccacggtcta ttcgggcggg tcctcgttga agaaccggcg
     3181 gtaggcaacc gcagcaagcc gggcggcgat caggctcgcc aaggcgctgc tcgcggtgag
     3241 catgacgctc caggtgagtc ttctgacgat gtcgttatcc attggtcaaa ccacaatacc
     3301 caagcctccg gagaacgaat acggaaacgg tccgcggggt cggaggatca cgggcgatcc
     3361 cgtggctacc tgaggttcaa caagacaggc aggaacaaac cctcggtcgg gggttcccgg
     3421 cgccgcccgg gcgtcagcgg cgttccaggc cttcgcagat cgccttgacc aggaggtccc
     3481 gggcttcgtc gaagtcacgg tcgctgccgg ggatcactcc gaatatccag ccggagagga
     3541 gctgctcgat cgcgccgtag aagaccatgg cggcgaaggc cgggtcgatg tcctcgcgga
     3601 agatgctctg tttctggccg tcggcgacga tccggccgat cagctcgtaa gcgcggttga
     3661 tctcctccag gtgcgtctgg ccgaaggaat tggccgcccg cgtgacctcg acgatgatca
     3721 ccttcatcag gtccgggtca tagcggtagg agtcgatgat gaaaccggcc accgcgccca
     3781 gcttctcctc gtgggcgagg gtgctgcggt cggcctcatc gatcgcctcg atcagaagcg
     3841 accagcgctg gatgaagagc tcgttgagga cgttctcctt ggaggagaag tagtggtaca
     3901 ccaggccgta ggcgacatcg gcctcatcgg cgatgtcggc cacccgggtg gagtggaagc
     3961 cctgacgggc gaagacgcgg accgccgcgt cgaggatctg gcgccggcgt tccggcgcgg
     4021 cctcacgagc catgagcgac tccttcactg aaaatttccg gccggcgatg gtttaccgcc
     4081 ggcagcttgc ggcggacctc cgcctgcctt tcgagatcga gttcggcaca ggcgaacccc
     4141 tccccctcct tcacttcggc cagcacctcg ccccagggac cgacgatcat cgaatgcccc
     4201 caggagtcgt acgaaggcgc ggcggtacca acctggttgg ccgccagaac gaatgcctgg
     4261 ttctcgatcg cccgggccct gagcagcgtc tcccagtggt ccctgccggt ggcgctggtg
     4321 aaggccgacg gcaccgtgaa cgcggtggcg ccgcgatcga gcagggcccg gaagagttcg
     4381 ggaaagcgca ggtcgtaaca gatggtcagg ccgattctga gatcgccggc gtcggccacc
     4441 gccagaccat ctccggcctg ctcgtggtcg gactcgcggt actccactcc gcccgcttcg
     4501 acgtcgaaca tgtggagctt gtcgtaggag cacacgatct gcccttcggg ggagatcagg
     4561 accgaggtgt tggtcggcag agccgaaggg ggaacgccac cggggtcggg gagcatcgtg
     4621 aagctgcccg ccagcaggtg gatgccgaga tccgcggccc agccacgggc ggccgtgatc
     4681 gcctctccgt cgagcgactc ggccccggcg gtgagagcag cgccatcccc gagcagtgcc
     4741 catttctctg gcagcacgac cagctctgcg ccgtcagctg cggcggcgac aaccagttcg
     4801 ccggcaactt cgaggttgcg ggagcggtcg gcacctgagt tgatctgtac gacggcagct
     4861 ctcatgatcg ggacttgatt gacacgtcaa tcaagaagga aagtataggt cgattaagaa
     4921 accggagcgc ccggcctcaa ggccgggcgc tccgaataca gcgggtgtgg cgggtgctcc
     4981 cctaccagcc cttcttcacg gccttggcac caccgttg
//
LOCUS       DAOQFI010000032         5013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_6802_length_5013_cov_3.476200, whole genome shotgun sequence.
ACCESSION   DAOQFI010000032 DAOQFI010000000
VERSION     DAOQFI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6802_length_5013_cov_3.476200"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLE93_01100"
     CDS             complement(<1..210)
                     /locus_tag="PLE93_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85672.1"
                     /translation="MASASATAAARPHVVTVRAPQKMAQGSAYVVATVIRNPAAKIAR
                     GSIRITLSTNRGSGPWLLGRREVAGV"
     gene            complement(371..1219)
                     /locus_tag="PLE93_01105"
     CDS             complement(371..1219)
                     /locus_tag="PLE93_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH85673.1"
                     /translation="MGENVRSEIFREDLLEGKVVVLSGAGTGLGREATLEMIRLGATV
                     VGCGRRAEPLEETAKLAGNLPGAFEHDTLDIRDEEGVDSFFDGVMERHGRIDTLVNNA
                     GGQFLTPAEAITPKGFRTVIELNVTGTWLMTHAAATKAFIPQGEGAVISVTLSPHNGM
                     PGMVHSGAARAAVENMMKTLSIEWARFGIRTCAIAAGQFATDTFVNKYPKEIVDVVHR
                     TIPMGRVGESEEMGWLIAFLASGAGAFHSGSVITLDGARDNWFGPWPPPSITGESGTP
                     PAEERR"
     gene            1270..2175
                     /locus_tag="PLE93_01110"
     CDS             1270..2175
                     /locus_tag="PLE93_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HNH85674.1"
                     /translation="MSIHAESRPLETPLPGGKEGATVTVEPMVTGTFSSPPILHDSPK
                     GKAGTLAILRSTRKEREPTPIPCFLIRHPSAGLILVDTGLHPSIASDPASNLGKFTTW
                     LMRPHQEPGQDVLSQLREKGINPADIRMVIFTHLHYDHASAISEFPEATLIVNDVEWK
                     YAATVRSPDFKGYRRQQFDYAFDFRTISFDSDKVSSYASFGRTLDLFGDGSIRLASTP
                     GHSPGHQSLICRLGDRDLVISGDAIYEESKLEAGANLPGTMFDEHNYKRSLSELKRFH
                     ETYPSAVITAGHETAFYENAPAVWE"
     gene            complement(2263..3036)
                     /locus_tag="PLE93_01115"
     CDS             complement(2263..3036)
                     /locus_tag="PLE93_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH85675.1"
                     /translation="MSELKPQKYKDNRPAEYFDHIHERSRNHEPNWVYEAVRAALTPI
                     AVGLYRTRAIGTENVPAEGPVLLAPNHFSNMDHFFAGVYLRRKIRFMAKSQLFGAPVL
                     TQIFKYGGVFPVRRGYKDEEAFKTVDAIFDQGGCVLVYAEGGRSRTGGLGEAKPGIGR
                     IALDSGVPVVPVAIHGSASVRKWKRLVLPKVTVQFGEPMTFPVIENATREQQLDCSKE
                     VFDQVKKMYTALDEKGRRGVKQALREKVKAPEPGRTIVP"
     gene            complement(3125..4630)
                     /gene="glpK"
                     /locus_tag="PLE93_01120"
     CDS             complement(3125..4630)
                     /gene="glpK"
                     /locus_tag="PLE93_01120"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNH85676.1"
                     /translation="MILAIDQGTTGSTALVFNDEGRICGRGYAEFRQHFPKPGWVEHD
                     AEEIWETSWGVAARALAGAGISGPDLDAIGITNQRETVVAWDRETGKPLHHALVWQDR
                     RGAARCDELKAVGLEGLVRERTGLVLDPYFSATKIEWLRQNVEGADRAVFGTIDSWLV
                     HRMCGEHLTDLTNASRTMLFDTGRLQWDPELCEVFGTDPASLPEARPSAGMFGTTSGF
                     GGEVPVAGIAGDQQAALFGQMCHEPGMAKNTYGTGSFVLLNAGDEKPDPGEGLLGTIA
                     WGIGNGAGSGAENKEVSVEYALEASVFVTGSAVQWLRDGLGIIKDASETEALAASLDS
                     NDGVYFVPALTGLGSPWWDPYARGTIVGLTRGTGRAHFARAALEAIAYQTVDAVRAQE
                     SASGEKLEALRADGGAVANKWLMQFQADLLGVPVEIPEISETTAFGAACLAGIGSGSW
                     TVEQVRGMWKPTARYEPKMNRDEAESLLNDWHRALESTLTASRAGSDPPSQ"
     gene            complement(4627..>5013)
                     /locus_tag="PLE93_01125"
     CDS             complement(4627..>5013)
                     /locus_tag="PLE93_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85677.1"
                     /translation="KRVGGGFGFALGFSAWVIRRRCGAEPQAPISGQRAMKLDVLRRV
                     LPFAPAYGMETGMTIDAVRAGYRLGEYEIDLAHKATGKSIKGFIHRFRQLLDFARVWW
                     ARRGGRGPDGKRPVESVAGSRPGATG"
BASE COUNT          821 a         1753 c         1550 g          889 t
ORIGIN      
        1 gaccccggcc acctcgcggc ggccaagcag ccaagggccc gaaccgcgat tcgtcgagag
       61 cgtgatcctg atcgaacccc gggcgatctt cgcggccggg ttgcgaatca ccgtggccac
      121 gacataggca cttccctggg ccatcttctg tggtgcccgg accgtgacca cgtgtggtct
      181 ggccgcagcc gtcgcagagg cggaggccat cgctccgaac agcaacagga gcgtcagcac
      241 aacagaccac ccgatcggcc tcctggtccg tgcttcgttg aagtccatcg tccagctcct
      301 tcccgcgcct ctgccagcct tcgtgatcag cataacgccg gccacatcgg ttccgggcga
      361 ttcgggagcg ttagcggcgt tcttcggccg gcggggtgcc gctctcgccg gttatcgagg
      421 gcggcggcca gggcccgaac cagttgtcgc gggcgccgtc cagggtgatc accgacccgg
      481 agtggaaggc accggcaccg gaggcgagga aggcgatcag ccagcccatc tcctcggatt
      541 ccccaacccg tcccatcggg atggtccggt gaaccacatc gacgatctcc ttcgggtact
      601 tgttcacgaa ggtgtcggtg gcgaactgac cggcagcgat cgcgcaggtc ctgatgccga
      661 atctcgccca ttcgatcgaa agcgtcttca tcatgttctc caccgcggcc cgggcggccc
      721 ccgagtgaac catgccgggc atgccattgt ggggagacag ggtgacgctg atcaccgcac
      781 cttccccctg gggaatgaag gccttggtcg cggcggcgtg ggtcatcagc caggtgccgg
      841 tcacgttcag ctcgatcacc gtgcgaaagc ccttgggggt gatcgcctcg gccggggtca
      901 ggaactggcc gccggcgttg ttgaccaggg tgtcgatccg gccgtgacgc tccatcacac
      961 cgtcaaagaa ggaatcgact ccttcctcgt cgcggatgtc aagggtgtcg tgttcgaagg
     1021 cgcccggcag attcccggcc agcttcgcgg tctcttcaag cggctccgct cgtcggccgc
     1081 aaccgaccac ggtcgccccc agccggatca tttccagggt ggcttcacgg ccgaggccgg
     1141 ttccggctcc ggagagcacg accaccttgc cctcgagcag gtcttcacgg aaaatctctg
     1201 agcgtacgtt ttctcccatt ggctgccgcg aatcctatcc ctgagccgtc cggcggtaag
     1261 gtccggcagg tgagcatcca cgctgagtcg agaccgctcg aaacgccgct tcccggcggg
     1321 aaggagggcg cgaccgtaac ggtcgagccg atggtgacgg gcaccttcag cagcccgccg
     1381 atccttcacg acagcccgaa aggcaaggcg gggactctgg ccatcctccg atcgacccgc
     1441 aaggaacggg aaccgacccc gattccctgt ttcctgattc gccacccttc cgccggactg
     1501 atcctggtcg acacgggact tcacccgtcg atcgcttccg acccggcttc caacctcggc
     1561 aagttcacga cctggttgat gcgccctcat caggagcctg gtcaggacgt tctctcccag
     1621 ctgcgagaga agggcatcaa ccccgccgac atccggatgg tgatcttcac tcaccttcac
     1681 tatgaccacg cctcggcgat ctccgaattc ccggaagcga ccctgatcgt caatgacgtc
     1741 gagtggaagt atgcggcgac cgtccgcagc ccggacttca agggttaccg ccgccagcag
     1801 ttcgattacg ccttcgactt ccggacgatc tccttcgatt ccgacaaggt cagctcgtac
     1861 gcaagcttcg gccgcaccct cgaccttttc ggggacgggt cgatccgcct ggcctcgact
     1921 ccgggtcact cacccggcca ccagtcgctg atctgccgcc tgggggaccg ggatctggtg
     1981 atcagcggcg acgcgatcta tgaagaaagc aagctcgaag ccggagcgaa cctgccgggc
     2041 acgatgttcg acgagcacaa ctacaagcgc agcctcagcg agctgaagcg cttccacgag
     2101 acctacccct cagccgtaat aaccgccggc cacgaaacag ccttctacga aaacgccccg
     2161 gccgtctggg agtagttatc ccgagacgtc aggcggggcc ggtggggact ccgacgacgt
     2221 ttcgggccga ggaggaggcc ccaccggccc cgcccccaag gctcagggca caatcgtccg
     2281 gccaggctcc ggcgccttca ccttctcgcg caacgcctgc ttcacgccgc gtcgcccctt
     2341 ctcgtcgagg gccgtgtaca tcttcttgac ctgatcgaag acttccttcg agcagtcgag
     2401 ctgctgctcc cgggttgcgt tctcgatcac cgggaaagtc atcggttcgc cgaactggac
     2461 ggtcaccttc ggcaacacca gtcgcttcca tttacggacc gacgccgaac cgtggatcgc
     2521 tacgggtaca accggaaccc cgctgtcgag ggcgatccga ccgatgccgg gcttcgcctc
     2581 accgagaccg ccggtccggg accggccgcc ctcggcgtag accagcacgc aacccccctg
     2641 gtcgaagatc gcatccactg tcttgaatgc ctcttcgtcc ttgtagccgc gccgcacggg
     2701 gaacactccg ccgtacttga agatctgggt cagtaccggg gccccgaaaa gttgtgactt
     2761 cgccatgaag cggatcttcc ggcgcaggta gaccccggcg aagaagtggt ccatgttcga
     2821 aaagtggttg ggcgccagca gtaccgggcc ttcggccggg acgttctcgg ttccgatcgc
     2881 ccgggtccgg tacaggccga ccgcgatcgg ggtcagtgcc gccctgaccg cctcgtagac
     2941 ccagttcggc tcgtggtttc tcgaccgttc gtggatgtgg tcgaaatact cggcgggccg
     3001 gttgtctttg tacttctgtg gcttcagttc ggacatgagt tagccggtct acccaaaggc
     3061 ctgcccgggg ttacacccga cggagccgac ccatccgtca ttttttctgt gggtcggcgg
     3121 gttctcactg ggagggcggg tcggaccccg cccgacttgc agttaaggtg ctctctaggg
     3181 ctctatgcca gtcgttcagg aggctttccg cctcgtcgcg gttcatcttc ggctcgtagc
     3241 gggcggttgg cttccacata ccgcgaacct gttcgaccgt ccagctgccg ctgccgatcc
     3301 cggccaggca ggccgcgccg aaggcggtgg tctcgctgat ttcgggaatc tcgaccggga
     3361 ctccgagcag gtcggcctga aactgcatca gccacttgtt ggccaccgcg ccgccgtcgg
     3421 cccgaagcgc ttcgagcttc tcgccggatg ccgattcctg ggcccggacc gcatccaccg
     3481 tctggtaggc gatcgcctcg agggccgccc gggcgaaatg ggcacgaccg gtgccgcggg
     3541 tcagcccgac gatcgtgccg cgggcgtatg gatcccacca gggagatccg agtccggtca
     3601 gggccggtac gaaatagacg ccgtcgttcg agtcgagcga cgccgccagg gcttcggtct
     3661 cgctggcgtc cttgatgatt ccgaggccgt cccgcagcca ctggaccgcc gacccggtca
     3721 cgaagacaga ggcttcgagg gcgtattcga ccgagacctc cttgttttcg gcaccggaac
     3781 cggcgccgtt gcctatcccc caggcgatcg ttccgagcaa gccctccccc ggatccggct
     3841 tctcgtcgcc ggcgttgagc agaacgaagc tgccggtgcc gtaggtgttc ttcgccatgc
     3901 ccggctcgtg acacatctgc ccgaacaggg cagcctgctg gtcaccggcg atcccggcca
     3961 ccggcacctc accgccgaag cccgaggtcg tgccaaacat tcccgccgag ggccgggcct
     4021 cgggcagcga ggcagggtcg gtcccgaata cctcgcagag ctccgggtcc cactggagcc
     4081 tgccggtatc gaacagcatg gtccgcgagg cgttggtcag gtcggtcagg tgctcgccac
     4141 acatccggtg gaccagccag gagtcgatcg ttccgaagac cgcccggtcc gcgccttcga
     4201 cgttctggcg cagccactcg atcttggtcg ccgagaaata agggtcgagg accagcccgg
     4261 tccgttcgcg aaccagtccc tccagcccca ccgccttcag ctcatcgcag cgggcagcgc
     4321 cccggcggtc ctgccagacc agggcgtgat gcagcggctt gccggtctcc cggtcccagg
     4381 caaccaccgt ctcgcgctgg ttggtgatcc cgatcgcgtc gaggtcggga ccgctgatcc
     4441 cggccccggc cagagcccgt gccgccaccc cccagctggt ctcccagatc tcctccgcat
     4501 cgtgctcgac ccaacccggc tttgggaagt gctgccggaa ttcggcgtag ccgcgaccgc
     4561 agatccggcc ctcgtcgttg aagaccagcg cggtcgaacc ggtggtcccc tgatcgatcg
     4621 cgaggatcat cctgtcgctc cgggcctgct cccggcgaca ctttcaaccg gtcgcttccc
     4681 gtccggtccg cggccaccgc gcctggccca ccagacccgg gcgaaatcga ggagttggcg
     4741 gaaccggtgg atgaacccct tgatgctctt tccggtcgcc ttgtgggcga ggtctatctc
     4801 gtactcgccg agccggtagc cggcccggac cgcgtcgatg gtcattccgg tctccattcc
     4861 gtaggcggga gcgaagggga gtacccgtcg cagcacgtcc agtttcatcg cccgctgacc
     4921 cgagatcggc gcctgtggtt cggccccgca gcgtcggcgg attacccagg ccgagaaccc
     4981 gagggcgaac ccgaagccgc caccgaccct ttt
//
LOCUS       DAOQFI010000033         4883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7133_length_4883_cov_3.232187, whole genome shotgun sequence.
ACCESSION   DAOQFI010000033 DAOQFI010000000
VERSION     DAOQFI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7133_length_4883_cov_3.232187"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2430)
                     /locus_tag="PLE93_01130"
     CDS             complement(<1..2430)
                     /locus_tag="PLE93_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HNH85678.1"
                     /translation="MNFPARIIARVVIVAAILGLGFASTAQADRSFSVRYTTNDTGSI
                     KGIANTNMTCPSSASGCSTAQTQDPTSSANSYYNNNYDMTYVDVDSDSSTFNSSTANQ
                     SLPSGSTILFAGLYWGGDYSTGSASAPNSSDRNKVKLKLPGSSTYTTITASTLDDSTL
                     NVGRYQAFADVTSLIQALPNAGNGTYGVANIQAGKGSDHYAGWTLIVAYRNTAETAKN
                     MSIFDGLVTIRPSDPPTTIPFSGFLTPPSGPVNPQIGFVTWEGDHGLVGDSASLNGNT
                     LSDAQHPATNFFDSRISYNGVLYTDRNPNYANALGIDAAWTTPPPGTVGNSETSATLR
                     VTTSGDQYLPGVITFQTEIYAPKIDQTKSVTDDNGGNVRQGDTLTYKIYAKNNGQDGT
                     SNFVLRDPIPPNTTYVPGSINITKNTNASTGAKTDASSDDIAEYDSANSRIVARLGEG
                     ATTSVGGNVKPDSEYEVTFKVKVNGPTVNPVPQGTVLTNTATASFNSRATGTPLTATS
                     TATATVTATPNLKITKVRTGAAFTAGGNSAYTLTVNNHGDAPTSGTVSVTDPIPSGLT
                     VTAISAPGWTCNSVPASSLSCSRSDALAAGASYPNIVLTVAIDSGVSGEVENTSTVSG
                     GGDSDLSDNTSSSTNPVSNVADLGLTKTASKDEVLIGENFTYALTVRNYGPSKATSVA
                     ITDELPAGLSFVSATPAPGCAIQPVTGTLGCEVGTLNSGASYTVTVTVHVDGSAHGVL
                     HNTATVGAQQTDPNPGNNSGSDDVEVLGADLQVTKVKKSPATVVTGNTVVYEIKVKNL
                     GPSNATGVVLHT"
     gene            complement(2878..3783)
                     /locus_tag="PLE93_01135"
     CDS             complement(2878..3783)
                     /locus_tag="PLE93_01135"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNH85679.1"
                     /translation="MEASPQYTLTERGPLRAALKTARPQEWVKNVLVFAGLIFSGQLD
                     DTWAIGQALITFACFCAISSAGYFINDLNDVELDRQHPKKRFRPIAAGQLKVSTAWIL
                     SVVLAAGAIGVAFGVVNWKVGCMVAGYGVIQVAYSFGLKQVVIIDVMTLATLFILRVM
                     AGAEAVDAHASEWLILCTGMLACFLGFTKRRQEAVSELHEGTSSRPVLEHYSLPFLDQ
                     MVAMVTTGTVVSYAIYTVNSPLAGSEMMLTIPPVVYGIFRYLYLIYDRSDDRSTAAIV
                     AEDRGVIIAGAVFVAVALLVLYAFD"
     gene            4092..4532
                     /locus_tag="PLE93_01140"
     CDS             4092..4532
                     /locus_tag="PLE93_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HNH85680.1"
                     /translation="MELTYEEGALTPEARDQLVENLTTNLLKAEGAPNTEFFRSVTWV
                     LINERPAALTYAGDKPGAGVVKLDVITPEGALNDDRREQMIQAGTATIREAFGIADED
                     ALKVWVLCRDVVDGGWGAGGNVIHYKELVEIAKAQRAEREAAAA"
     gene            4600..>4883
                     /locus_tag="PLE93_01145"
     CDS             4600..>4883
                     /locus_tag="PLE93_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNH85681.1"
                     /translation="MTTSTREAMLGSAIRLFRERGVGDTSFADVLAASGAPRGSVYHH
                     FPGGKKQLVQEAIESAGGYVAANFERSEDGPAEMVDRFARFCEVAGKALG"
BASE COUNT          828 a         1444 c         1705 g          906 t
ORIGIN      
        1 cgtgtggagg acgacgccgg tggcgttcga gggaccgagg ttcttcacct tgatctcgta
       61 gacgacggtg ttaccggtga ccacggtggc cggagacttc ttgaccttgg tgacctgaag
      121 gtccgcgccg agcacttcga cgtcatccga accgctgttg ttgcccgggt tcgggtcggt
      181 ctgctgggcg ccgaccgtcg cggtgttgtg aagcacaccg tgagcggaac cgtcgacgtg
      241 gactgtcacg gtgacggtgt agctcgcccc ggagttcagg gtgccgactt cgcaaccgag
      301 cgttcccgtg accggctgga tcgcacagcc gggagctggg gtggccgaga cgaagctgag
      361 gccggccggc aactcgtcgg tgatcgcaac cgaggtcgcc ttcgagggtc cgtagttcct
      421 gaccgtgagg gcgtaggtga agttctcgcc gatcagaacc tcgtccttgg acgcggtctt
      481 ggtcaggccg aggtcggcga cgttcgagac cgggttggtc gagctcgagg tgttgtcgct
      541 caggtcggag tcaccgccgc cggagacggt cgaggtgttc tcgacctcac cgctgacgcc
      601 gctgtcgatc gcgacggtca ggacgatgtt cgggtagctc gcaccggcag ccagcgcatc
      661 gctgcgcgag caggaaagcg aactggccgg gacgctgttg caggtccagc cgggcgcgct
      721 gatcgcggtt acggtcagtc cggaggggat cggatcggtc acggagaccg tacccgaggt
      781 gggagcgtcg ccgtggttgt tgacggtcag cgtgtaggcg ctgttgccgc ctgccgtgaa
      841 ggccgcacca gtgcgcacct tggtgatctt caggttcggc gttgccgtca ccgttgcggt
      901 ggcggtcgag gtggcggtca gcggggtgcc ggtggcccgg ctgttgaagg aagccgtcgc
      961 ggtgttggtc aggaccgtgc cctgcgggac cggattgacg gtcgggccgt tgaccttgac
     1021 cttgaaggtg acctcgtatt cgctgtccgg cttcacgttg ccaccgaccg aggtcgtcgc
     1081 accctcgccc aggcgggcga cgatccggct gttggccgag tcgtattcgg cgatgtcgtc
     1141 cgagcttgcg tcggtcttgg cgccggtcga ggcgttggtg ttcttggtga tgttgatcga
     1201 gccgggcacg taggtggtgt tcggcgggat cggatcgcgc aggacgaagt tcgaggtgcc
     1261 gtcctggccg ttgttcttcg cgtagatctt gtaggtcagg gtgtcgccct ggcgcacgtt
     1321 gccgccgttg tcgtcggtga ccgacttggt ctggtcgatc ttcggcgcgt agatctccgt
     1381 ctggaaggtg attacgccgg gcaggtactg gtcacccgag gtggttaccc gcagggtcgc
     1441 cgaggtttcg ctgttgccga ccgtgcccgg gggcggggtg gtccaggcgg cgtcgatgcc
     1501 gagcgcgttt gcgtagttcg ggttgcgatc ggtgtagagc acgccgttgt acgagatccg
     1561 cgaatcaaag aagttggtcg ccggatgctg ggcatcggag agcgtgttgc cgttcaggga
     1621 agccgaatct ccgaccaggc cgtggtcacc ctcccaggtg acgaatccga tctgggggtt
     1681 gaccggaccc gaaggcggcg tcaggaagcc cgagaaagga atcgtggtcg gcgggtccga
     1741 gggacggatg gtgaccagac cgtcgaagat cgacatgttc ttggccgttt ccgcagtgtt
     1801 ccggtaagcg acgatcaggg tccagccggc gtagtgatcc gagcccttgc cggcctggat
     1861 gttggccacg ccataggtgc cgttgcccgc gttgggcagc gcctggatga ggctggttac
     1921 gtcagcgaag gcctggtaac ggccgacgtt cagggtcgag tcgtcgagcg tgctggccgt
     1981 gatcgtggtg taggtgctcg atccgggaag cttgagcttg accttgttgc ggtcgctcga
     2041 gttgggcgcg gacgccgagc cggtgctgta atcgccgccc cagtagagac cggcgaagag
     2101 aatcgtcgat ccggaaggca ggctctggtt agccgtcgag gagttgaagg tcgaactgtc
     2161 ggaatcaacg tcgacatagg tcatgtcgta gttgttgttg tagtacgagt tcgccgagga
     2221 ggtggggtcc tgggtctgtg cggtcgaaca gcccgatgcg ctcgaggggc aggtcatgtt
     2281 ggtgttcgcg atgcccttga tcgacccggt gtcattggtc gtgtagcgaa cgctgaagct
     2341 gcggtcagcc tgggcagtgg aagcgaagcc gaggccgaga atggcggcga cgatgacgac
     2401 gcgggcgatg attcgagcgg ggaaattcat tcagttctcc gaggtttctc ttttgggtac
     2461 accggtttcg ttcgagccat ttcctggccc ggctgaacgg tgggaaagcc tcgaaggccc
     2521 ggtcaactgc gcaagtatcg agattcagca gttgacagag tgtcttttct ggatccctgc
     2581 cggtttccaa ccggctctcg aagggggtcc gcacccgtct cccgtgttct tattggtcga
     2641 ttgagaatac gcgttttctg atgcgtgtca agtgtccgga ccgactgata tgaaaaatgc
     2701 gaattacccg caatcggcgg tccagcacgg gtttaccggt tatcgggctc ggcggactgc
     2761 cttgaatgtg cccgcaaata gccgggattt gcctgctttg ccggcttgag ccggtcgacc
     2821 gcaagtcagg cacctccggc ccgaatcgga cgaccggatt ctgcccgccg gcctggatca
     2881 gtcgaaggcg tagaggacca gcagggcgac tgcaacgaag accgccccgg cgatgatcac
     2941 tccccggtcc tcggccacga tcgccgcggt tgaacggtca tccgagcggt cgtaaatcag
     3001 atagaggtag cggaagatgc cgtagacgac ggggggaatc gtcagcatca tctcggaacc
     3061 ggccaggggc gagttcacgg tgtagatcgc gtaactgacc acggtgccgg tagtcaccat
     3121 ggcgaccatc tggtcgagga agggcaggga gtagtgctct agcaccggtc ggctgcttgt
     3181 tccctcgtga agttcggata ccgcttcctg gcgccgcttg gtgaagccga ggaagcaggc
     3241 cagcatgccg gtgcagagga tcagccactc cgaggcgtgg gcatccacgg cctcggcgcc
     3301 ggccatcacc cggaggatga acaacgtggc gagggtcatc acatcgatga tcacgacctg
     3361 tttgaggccg aagctgtagg cgacctggat cacgccgtaa ccggcgacca tgcagccgac
     3421 cttccagttg accaccccga aggcgacccc gatcgcaccc gcggcgagca ccaccgagag
     3481 aatccaggca gtgctgacct tgagctgccc cgccgcaatc ggccggaacc gcttcttcgg
     3541 atgctgacgg tcgagctcga cgtcgttcag gtcgttgatg aagtagccgg cgctggagat
     3601 cgcgcagaag caggcgaagg taatcagggc ctggccgatc gcccaggtgt catcgagctg
     3661 accggagaag atcaggccgg cgaagaccag gacgttcttg acccactcct ggggccgcgc
     3721 cgtcttcagg gcggctcgca acggtccgcg ctcggtcagt gtgtattggg gggaggcttc
     3781 catggtcggg gttcgcatcc cgactgcggg acacggccgg ggaaggctac cggctcaggc
     3841 cccggttcaa cctgttcatc gccagatgca cgcctcagtc catcgaaagc gccgcggcca
     3901 accgaccccg acccgtcgag atcccgtttt gcgccacata ccggcacaaa gtgggcactc
     3961 ggtgaatctt cccggcaaga actccgcccc ggcacttgac tatgcacccc tgcatagggt
     4021 atgcatacct gcatagtcgg ctcaatcccg agccgatcct tcccggacca aggagaaatc
     4081 caatgccttt gatggaactg acttacgaag aaggggccct gacgcccgag gcccgcgacc
     4141 agctggtcga gaacctgacc accaacctgc tcaaggccga gggtgccccg aacaccgagt
     4201 tcttccgcag cgtgacctgg gtcctgatca acgagcgacc cgccgcgctc acctacgccg
     4261 gtgacaagcc cggagcaggt gtggtgaagc tggacgtgat cacgcccgag ggcgcgctca
     4321 acgacgaccg gcgcgagcag atgatccagg ccggcaccgc cacgatccgc gaggccttcg
     4381 gcatcgccga cgaggatgcc ctcaaggtct gggtgctttg ccgcgacgtg gtggacggcg
     4441 gatggggcgc gggcggcaac gtcatccatt acaaggagct ggtcgagatc gccaaggccc
     4501 agcgggccga acgggaagcc gcggcggcct gaccctgagg cgccggctgg ccgcacagcc
     4561 agccggcgcc ccgccatccg gaacggatct ctttcgacca tgacaaccag cacccgcgaa
     4621 gcgatgctcg gctcggcgat ccgcctcttc cgcgagcggg gggtcggcga cacctccttc
     4681 gccgacgtgc tcgccgcgag cggcgccccc cgggggtcgg tctaccacca ctttccgggc
     4741 ggcaagaagc agctcgtcca ggaagcgatc gagtcggccg gcggctatgt cgccgccaat
     4801 ttcgaaagga gcgaggacgg gccggccgag atggtcgacc gcttcgcccg cttctgcgag
     4861 gtggccggca aggccctggg cga
//
LOCUS       DAOQFI010000034         4872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7163_length_4872_cov_3.194727, whole genome shotgun sequence.
ACCESSION   DAOQFI010000034 DAOQFI010000000
VERSION     DAOQFI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7163_length_4872_cov_3.194727"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLE93_01150"
     CDS             <1..633
                     /locus_tag="PLE93_01150"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNH85682.1"
                     /translation="PVGLRFLLASTSEVYGDPLVHPQPEAYWGNVNPIGPRGCYDEAK
                     RFAEAITMAYHRAHGLDTRIVRIFNTYGPRMRPGDGRVVSNFIVQALRGEPLSVYGDG
                     AQTRSFCFVDDLVRGLIALAESGEHLPVNIGNPDEYTLLQLANAVIEASGAESTITFE
                     PLPTDDPQVRQPNIDRAKELLGWEPTVPLSEGLKKTIEQSGRDRLIGKTG"
     gene            669..1532
                     /locus_tag="PLE93_01155"
     CDS             669..1532
                     /locus_tag="PLE93_01155"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH85683.1"
                     /translation="MLGASGFFGSRIAAELAAAGIEVLGPERSESELLDPAGLSELIA
                     AAAPAAVVNAAGMTSPASARANPSACFAVNAGGALNLLEALRQRAPGAQLVSLSSAAV
                     YAGSAPFDESSATAATTPYAASKLAMETICGQYRRGYGIPVTVLRCFNLIGPGEPSTQ
                     ASSEFVRAALAAGTGGRAEVEVGEPATARDFTDVRDAARAVRRVLEEGATGTLNLCSG
                     KATSLAELAALTGELTGVDLTLRGSGTGRPASGLLSIRGDFSKLRELTGWRPGITLER
                     SLTDLIESLRP"
     gene            1544..2161
                     /locus_tag="PLE93_01160"
     CDS             1544..2161
                     /locus_tag="PLE93_01160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH85684.1"
                     /translation="MAANSTETVDTTNYEKFQTGNPVVKRLIGGFYDTVEEVVKPLKP
                     ATVLDAGCGEGETLDRLSEVLPPAPTGIDLNPESVEFAARRLPGATIRQADLLNLPFE
                     DGSFDLVFCLEVLEHLPDPAAGLAELARVSGRDIVVSVPHEPWFRLGSFARGKYLKTW
                     GNHPEHVNHWNPKSFRTFLSTRTEVVEVQTSMPWIIARCRPLPAG"
     gene            complement(2127..3116)
                     /locus_tag="PLE93_01165"
     CDS             complement(2127..3116)
                     /locus_tag="PLE93_01165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH85685.1"
                     /translation="MDLSLIIPAYNESEALPGLIAEIDSACEGLGLQWETIVIDDGST
                     DGTFGVISEIAKSNPRVGVVRLRRNFGKSVALAVGFDVTRGEKVITIDGDGQDDPADI
                     PRLLEGLENGSDLVSGWKSDRQDPVTRRLASKIFNWFTARVTGVDLHDMNCGFKAYRG
                     ECARSIEVYGEMHRFLPAFAAQQGWRVTELPVNHRPRLHGSSRFGLERYRRGALDLLT
                     VAFLGRYQNRPLHLFGLLGLVLGSLGFLICLYLSIIWIGGEAIGEPPLLFLGVLLIVV
                     GVQLFSLGLLGQMMVVGRRAGVSEEKVLIEKVLSPESDPELGLSRLEAVGSGR"
     gene            3149..4594
                     /locus_tag="PLE93_01170"
     CDS             3149..4594
                     /locus_tag="PLE93_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85686.1"
                     /translation="MTHPRELIRRTFGNPDEGTAGRKARLAGLIGVPVAGAFLIGVIL
                     GVIGQSLSAGATFILLAIPVAIGGGILTGRAVLGGAMQLRGNQKAGWITEKLEPLNVA
                     ADPGQPLRVEIIHPAVDLKHFFGGFIAVFNLARRLGEGGHRVRIVTLEPLDLPDDWRR
                     QVAAYQGIGDSVGDLEVIDGSDRAVPVAMNPDDRLIATHWTAAHIASAAAAELDQESF
                     TYLIQEYEPLIFPMGSAAALADQSYELDHNAIFSTGFLRDYFRDQGIGVYRPEGPGEA
                     GAAVFDNAITPAATVTTGELQGKDRRRLLFYARPEAHAERNLFELGIMALDRAILAGH
                     FRGWDLAGIGNVDGTGELTLPQSKARLRMLPRVAQREYADTLRQFDVGLALMYTPHPS
                     LVPLEMAAAGLVTVTNTYANKDRASLEAISGNLIAAEPRIDQIEEALASAEVMSRDFE
                     ARVEGSKVGWPDNWDQALDDATMERIGRLLD"
     gene            4619..>4872
                     /locus_tag="PLE93_01175"
     CDS             4619..>4872
                     /locus_tag="PLE93_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85687.1"
                     /translation="MAEQGKSTDEKIDAVREGYESTLSWRVTRPLRSISDAARRLRGR
                     ETEIPWAEVMVEEKLEPDWTSWLDTFFGEELERIDAALAET"
BASE COUNT          825 a         1675 c         1636 g          736 t
ORIGIN      
        1 ccagtaggcc tccgcttcct gctcgcctcg acctccgagg tgtacggcga cccgctcgtg
       61 catccgcagc cggaggccta ctgggggaac gtcaacccga tcggtccccg cggctgctac
      121 gacgaggcga agcgcttcgc cgaggcgatc acgatggcgt accaccgcgc ccacggcctc
      181 gatacgcgga tcgtgcggat cttcaacacc tacggccccc ggatgcggcc cggcgacggg
      241 cgggtcgtct cgaacttcat cgtgcaggcg cttcgcggcg agcctctctc ggtgtacggc
      301 gacggggcgc agacccggtc cttctgcttc gtcgatgacc tggtccgcgg cctgatcgcg
      361 ctggccgaat cgggggagca cctgccggtc aacatcggca accccgacga gtacacgctg
      421 ctgcagctcg cgaacgcggt gatcgaggct tccggggccg aatcgacgat caccttcgag
      481 ccgcttccga ccgatgaccc gcaggtccgc cagcccaaca tcgaccgggc gaaggagctg
      541 ctcggctggg agccgaccgt gcccctgtcc gagggcctca agaagacgat cgaacagtcg
      601 ggccgcgacc ggctgatcgg caagaccggc tgaattccag gaccgtgacc gggcccggga
      661 gagttctcgt gctcggggct tccggctttt tcgggagccg gattgcggcc gaactcgccg
      721 cggccggaat cgaggtgctc ggtccggagc ggagcgaatc cgagctgctc gatcccgccg
      781 gcctcagcga gctgatcgct gccgccgcac cggcggccgt ggtcaacgcc gcggggatga
      841 cctcaccggc atcggcccgg gcaaaccctt ccgcctgctt cgcggtcaat gcgggcgggg
      901 cgctgaacct gcttgaagcg ctgcggcaac gggcccccgg ggcgcagctc gtgtcccttt
      961 cctcggccgc ggtctacgcc gggtcagcgc ccttcgacga atcttcagcg accgcggcga
     1021 ccacgcccta cgccgcttcg aagctggcca tggagacgat ctgcggccag taccgacgcg
     1081 gctacgggat accggtgacg gtgctgcgct gcttcaacct gatcggcccc ggcgagcctt
     1141 caacccaggc gagttcagag ttcgtccggg ccgccctggc cgccgggact ggcggacggg
     1201 ccgaggtgga ggtcggggag ccggccaccg ccagggactt caccgacgtg cgcgacgccg
     1261 cccgggcggt ccgccgggtt ctcgaggaag gagcaaccgg caccctcaac ctctgctcgg
     1321 gaaaggcgac gagcctggcg gaactggccg ccctgaccgg cgagctgacc ggggttgacc
     1381 tgaccctgcg cggctccgga accggacggc ccgcgtccgg gctgctttcg atccgcgggg
     1441 acttctcgaa gcttcgcgag ctgaccggct ggcgccccgg gatcacgctt gagcgcagcc
     1501 tcacggacct catcgagagc ctccgcccat aggatcaccc gccatggcag cgaacagcac
     1561 cgaaaccgtc gacaccacca actacgagaa gttccagacc gggaacccgg tggtgaagcg
     1621 gctgatcggc ggcttctacg acacggtcga ggaagtcgtg aagccgctga agccggccac
     1681 cgtgctcgat gccggatgcg gcgaagggga aaccctggat cgcctgagcg aggttctgcc
     1741 gcccgccccc accgggatcg acctcaaccc cgagtcagtc gagttcgccg cccggcgact
     1801 ccccggcgcg accatccggc aggccgacct gctcaacctg ccgttcgagg acggcagctt
     1861 cgacctcgtc ttctgcctcg aggtgctcga acacctgccc gacccggctg ccggcctggc
     1921 cgaactggcc cgggtttccg gccgggacat cgtcgtttcg gtgccgcatg agccctggtt
     1981 ccggctcggc agcttcgccc gcggcaagta cctcaagacc tggggcaacc atcccgagca
     2041 cgtgaaccac tggaatccga agagcttccg gaccttcctc tcgacccgga ccgaagtggt
     2101 cgaggttcag acctcgatgc cgtggatcat cgcccgctgc cgaccgcttc cagccggctg
     2161 agaccgagtt ccggatcgct ttcggggctg agcaccttct cgatcagcac cttctcctcg
     2221 gagacaccgg cccgccggcc gaccaccatc atctggccga gcaggcccag tgagaacagc
     2281 tggaccccga ccacgatcag cagcaccccg aggaagagca gcggtggctc gccgatcgcc
     2341 tcgccaccga tccagatgat cgagaggtag aggcagatca gaaaaccaag cgagccgagg
     2401 acgagcccga gcaggccgaa aaggtgcagg ggacggttct ggtagcggcc gagaaaggcg
     2461 acggtcagca ggtcgagggc gccgcgccgg tagcgctcga ggccgaagcg ggaagagccg
     2521 tggaggcggg gacggtgatt taccggcagc tcggttaccc gccagccctg ctgggcggcg
     2581 aaggccggca ggaagcggtg catctccccg tagacctcga tcgagcgggc gcattcaccg
     2641 cggtaggcct tgaagccgca gttcatgtca tgcaggtcga ctccggtcac ccgggcggtg
     2701 aaccagttga agatcttcga cgccaggcga cgggtgacgg gatcctgacg gtcgctcttc
     2761 cagcctgaga caaggtcgga cccgttttcc aggccttcga gcagtcgcgg gatgtcggcc
     2821 gggtcatcct ggccatcacc gtcgatcgtg atcaccttct cgccgcgggt aacgtcaaag
     2881 ccgaccgcca gggcgaccga cttgccgaag ttccgcctca gcctgacgac cccgacccgg
     2941 ggattggact tcgcgatctc cgagatcacc ccgaaggtgc catcggtcga gccatcatcg
     3001 ataacgatcg tctcccactg aaggccgagc ccttcgcagg cggaatcgat ctcggcgatc
     3061 agcccgggca gggcttcgct ctcgttgtag gcgggaatga tcagggacag gtccattgct
     3121 tgcccgtcaa ggtaatacgc tgcgtcgcgt gacgcacccc cgggagctga tccgacggac
     3181 ttttggcaac cccgatgaag gcaccgccgg acgcaaggcg aggctggccg ggctgatcgg
     3241 cgtgccggtt gccggggcct ttctgatcgg cgtgatcctc ggggtgattg gccagagcct
     3301 ctcggccggc gcgaccttca tcctcctggc gattccggtg gcgatcggcg gcggcatcct
     3361 gaccggccgg gccgtgctgg ggggagcgat gcagctgcgc ggcaaccaga aggccggctg
     3421 gatcaccgag aaactggaac cgctgaacgt cgcggccgac cccggccagc ctttgcgggt
     3481 cgagatcatc caccccgccg tcgacctcaa acatttcttc ggtggcttca tcgcggtctt
     3541 caacctggcc cggcggctgg gcgagggcgg ccaccgggtc cggatcgtca ccctggagcc
     3601 actcgacctg cccgatgact ggcgccggca ggtcgccgcc tatcagggga tcggtgactc
     3661 ggtgggtgac ctggaagtga tcgatggcag cgatcgggcc gtcccggtcg cgatgaatcc
     3721 ggatgaccgc ctgatcgcca cgcactggac tgccgcccac atcgcctcgg ctgctgccgc
     3781 ggaactcgat caggagtcct tcacttacct gatccaggaa tacgagccgc tgatctttcc
     3841 gatgggcagc gccgccgccc tcgccgatca gagctacgaa ctagaccaca acgcgatctt
     3901 ctcgaccggg ttcctgcggg actacttccg cgaccaggga attggcgtgt accggccgga
     3961 aggaccgggg gaagcgggcg ccgcggtctt cgacaacgcg atcaccccgg cggccacggt
     4021 caccaccggc gagcttcagg gcaaggaccg gcgccggctc ctgttctacg cgcgccccga
     4081 ggcccacgcc gaacgcaacc tcttcgagct ggggatcatg gccctcgacc gggcgattct
     4141 cgctggccat ttccggggct gggacctggc cgggatcggg aacgtggacg ggaccgggga
     4201 gctgaccctg ccgcagtcga aggcccggct gcggatgttg ccgcgggtcg cccagcggga
     4261 gtacgcggac acgctacgcc agttcgacgt cggcctggcc ctgatgtaca ccccgcatcc
     4321 gagcctggtc cccctcgaga tggcggccgc cgggctcgtc accgtcacca acacctacgc
     4381 gaacaaggac cgggcttcac tcgaggcgat ttcgggcaac ctgatcgccg ccgagccgcg
     4441 gatcgaccag atcgaggaag cgctggcctc ggccgaagtg atgagccggg acttcgaggc
     4501 ccgggtcgaa ggctcaaagg tggggtggcc cgacaactgg gaccaggccc tggacgacgc
     4561 aacgatggaa cggatcgggc gcctgctcga ctagacaacc tctagggttg cccctcaggt
     4621 ggccgaacag gggaagagca ctgacgagaa gatcgacgcg gtccgggagg gctacgagtc
     4681 aaccctgagc tggcgggtga cccgtccgct ccggtcgatc agcgatgccg cccggcggct
     4741 gcgcggccgc gaaacggaga ttccctgggc cgaggtgatg gtcgaggaga agctcgaacc
     4801 ggactggacc tcctggctcg acaccttctt cggtgaggag ctggaaagga tcgacgctgc
     4861 cttagccgag ac
//
LOCUS       DAOQFI010000035         4869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7170_length_4869_cov_3.507063, whole genome shotgun sequence.
ACCESSION   DAOQFI010000035 DAOQFI010000000
VERSION     DAOQFI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7170_length_4869_cov_3.507063"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..970
                     /locus_tag="PLE93_01180"
     CDS             98..970
                     /locus_tag="PLE93_01180"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNH85688.1"
                     /translation="MLIGAHVSTSGGLAKAVSRGEEIGCRSIQIFNQSPRAWKPTEYS
                     EEDFAEFREAMAASQVESVIIHAIYLINCVTPDRDLARKSRKALIHALRVGDGIGADG
                     VVLHPGSRKQEEVVPAIVRGGGLIEEALSESESCPLLIEQMAGHQNILAHTFEEIAGL
                     IEAAGGDRRLGLCLDSCHLFASGYDIGTYQGIDRMLKESDKAVGKRRLKALHLNDSKE
                     PLGSRRDRHEDIGKGEIGETGFARFLSEPRFEGLPATMETPGPDRKGPVAADIELAEK
                     LKRRGRAARRRRNS"
     gene            1081..2475
                     /locus_tag="PLE93_01185"
     CDS             1081..2475
                     /locus_tag="PLE93_01185"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HNH85689.1"
                     /translation="MTPSDPTPQQKLNPEQQEMMVESFFTRPAADRKIPELGVIPRAA
                     YNMVASEMELDGDPNKNLATFVTTWMEPEARQVITDSLHKNYIDHAEYPRTAEISKRC
                     VRMIHDLFNGPEGQDAPGTACAGSSEAVMLGALSMKWNWKNRQEKAGKDATRPNLVYG
                     SDVHVVWDKFCRYFDVEPRNIPIPKGKTVMGPDDFREHIDENTIGVIGVVGTTFTGQC
                     DDVVGIDQMLTEFKGKGLEVPMHIDAASGGFVFPFSHPDFEWDFRLDSVVSINVSGHK
                     FGLVYPGIGWLITRTDAQVAEDLIFYEDYLGEKDATFTLNFSGSSSFVLAQYYQFLRL
                     GHSGYSSMVRAMTKNREALADRLRDMDALTVYEDDSPTLPLLIAKTNDNEPFDSNDLV
                     GELARRRGWLVPAYQMPPNNENDRIMRMLVKFNQTRELVDALCDDFEASISFLRKRGE
                     GKVDSPPAHTGHGY"
     gene            complement(2751..4013)
                     /locus_tag="PLE93_01190"
     CDS             complement(2751..4013)
                     /locus_tag="PLE93_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH85690.1"
                     /translation="MTATETSEETTVDESSEPKFSLELNQDQKDIQEWVHGFAENVMR
                     PAAHEWDEREEFPWEIVQEAAKIGLYGFEGTAQFFADPTGLSLPIVNEELFWGDAGLG
                     MAIQGTGLAVASIFGQGTPEQMGEWVPQCYGTEDDVKIAAFCSSEPDAGSDVSNVRTT
                     AKYDEAKDEWVINGQKAWATNGGIANIHVVIASVDRELGSRGHAAFIIPPNTPGCSQG
                     AKVKKHGIRASHTADVHLDDCRVPGSCLLGGKEKLDERLARVREGKKGKSQAAMATFE
                     ASRPIVGSQAIGIARAAYEYALEYAKERVQFGRPIIENQAIAFTLADMCLEIDAARLL
                     VWRASWMGRNQIPFKNSEGSMSKLKGGEVATWVTERAIQILGGNGYTREFPVERMHRD
                     AKIYDIFEGTAEIQRLVISRSISGIKIK"
     gene            complement(4155..>4869)
                     /locus_tag="PLE93_01195"
     CDS             complement(4155..>4869)
                     /locus_tag="PLE93_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH85691.1"
                     /translation="TAIPFKDEAEALVLANDVQYGLTGYLWTADITRAFRFTDHLDAG
                     MIWVNSENVRHLPTPFGGVKNSGIGRDGGDWSFDFYMETKNIAFATLSDIEHDIELML
                     FNLEDSEKQQLVQMDEVVMVRGTVDQSEEGSKLKVRDAELFNPSEAQIEKAKIAYAKK
                     IEPFVVRVVPEQMSLDTLEDMKSVFGQHQGESDVVVVVEGDREHRLKLGPDFRVRHSP
                     GLATEIDQIFGDGTAARAA"
BASE COUNT          892 a         1555 c         1545 g          877 t
ORIGIN      
        1 gaaaagcggc ctccgggtca gattcgcacc ttctcccccg atgtgtagtg cgccggtggg
       61 agaaggtgcg aatctgaccc ggaggccgct tttctcaatg ctcatcggtg cccatgtttc
      121 cacttccggc ggcttggcga aggccgtgtc ccggggggaa gagatcggct gccgctcgat
      181 ccagatcttc aaccagagtc cgcgggcctg gaagccgacc gaatactcgg aggaggactt
      241 cgccgagttc cgcgaggcga tggccgcgag tcaggtcgag agcgtgatca tccacgcgat
      301 ctacctgatc aactgcgtca cgccggaccg tgacctggcc aggaagtcgc gcaaggcgct
      361 gatccacgcg ctgcgggtcg gcgacggaat cggggccgac ggcgtggtcc tccaccccgg
      421 ttcacgcaag caggaagagg ttgtgccggc gatcgtccgg ggcggcggtt tgatcgaaga
      481 ggccctttcg gagagcgaaa gctgtccgct cttgatcgaa cagatggccg gccaccagaa
      541 catcctcgcc cacaccttcg aggagatcgc cggattgatc gaggcggccg gcggcgaccg
      601 gcgactcggg ctctgcctgg attcctgcca tcttttcgcg tcggggtacg acatcggtac
      661 ctaccaaggc attgaccgga tgctgaagga gtcggacaag gcggtcggca agcggcgcct
      721 gaaggctctt cacctgaatg attcgaagga gccgctcggc agccgccgcg accgtcatga
      781 ggacatcgga aagggcgaga tcggcgaaac cggattcgcc cgattcctct cggaaccgag
      841 attcgaaggc ctgcccgcaa cgatggagac cccggggccg gacaggaagg gaccggttgc
      901 cgccgacatc gaactcgccg aaaagctgaa gcggagggga cgggccgctc gccgtcgccg
      961 caattcgtag ggcctgctcc gttgatcatc cgcgccggat cggggatcat ccgatcggca
     1021 tgaacggaca gtgacaggca gcggataggt ttttgggaaa ccgatcgtcg aaggagatag
     1081 atgaccccgt cggaccccac accccagcag aagctgaatc ccgaacagca ggagatgatg
     1141 gtcgagagct tcttcacccg gcctgctgcg gaccgcaaga ttcccgagct tggtgtcatc
     1201 ccccgggccg cctacaacat ggtcgcctcg gagatggaac tcgacggtga cccgaacaag
     1261 aacctggcga ccttcgtcac cacctggatg gagcccgaag cccgccaggt gatcactgac
     1321 agcctgcaca agaactacat cgaccacgcc gagtacccgc gcaccgcgga gatctccaag
     1381 cgatgcgtgc gcatgattca cgacctcttc aacggtcccg aaggccagga tgctccgggc
     1441 accgcctgtg ccggctcgtc cgaggcggtc atgctcggcg cgctttccat gaagtggaac
     1501 tggaagaacc gccaggagaa ggccggcaag gacgcgaccc ggcccaacct cgtctacggg
     1561 tcggacgttc acgtggtctg ggacaagttc tgccgctact tcgacgtcga gccccgaaac
     1621 atccccattc cgaagggcaa gacggtcatg gggccggacg acttccgcga gcacatcgac
     1681 gagaacacga tcggggtgat cggcgtcgtc ggcactacct tcaccggtca gtgcgatgac
     1741 gtggtcggca tcgaccagat gctgaccgag ttcaagggca agggcctcga ggtgccgatg
     1801 cacatcgacg ccgcttcggg cgggttcgtc ttcccgttct cgcaccccga cttcgagtgg
     1861 gatttccggc tcgactcggt cgtttcaatc aacgtctcgg gccacaagtt cggcctggtc
     1921 taccccggga tcggctggct gatcacccgg accgacgccc aggtggccga agacctgatc
     1981 ttctacgagg actacctcgg ggagaaggac gccaccttca ccctgaactt ctcgggaagc
     2041 tcgtccttcg tcctcgccca gtactaccag ttcctgcggc tcggtcattc gggctacagc
     2101 tcgatggtca gggcgatgac caaaaaccgc gaggcgctcg ccgaccggct ccgagacatg
     2161 gatgcgctca ccgtctacga ggacgacagc ccgaccctgc cgctgctgat cgcgaagacg
     2221 aatgacaacg agccgttcga cagcaacgac ctggtcggag aactcgcccg ccgtcgaggc
     2281 tggctcgtgc cggcgtacca gatgcccccg aacaacgaga acgaccggat catgcgcatg
     2341 ttggtcaagt tcaaccagac ccgtgaactg gtcgacgcgc tctgcgacga tttcgaagcc
     2401 tcgatctcct tcctgcgcaa gcgcggagaa ggaaaggtcg atagcccccc ggcccacacc
     2461 ggccacggct actgagaaac cagatccgcg acccgcgggg tcgcttccgc gctccggcag
     2521 cgggtggtta cgccgtgggg ccgggtggcc ctcccaaaga tgcctctacc gtcgcgtgaa
     2581 cgagtgcctg ctcgacggtc ggagcagtaa tcgggaaggc actttgggag ggccacccga
     2641 ccccgcggct actccagatc gggagatgcc tcccctcgcc cgcctttgaa gtccgaggtg
     2701 ccgcaggttt gcgacctctg gggtcgtaaa cctgcggcac ctcgtttccc ctacttgatc
     2761 ttgattcccg agatgctgcg ggagatcacg agacgctgga tttccgcggt gccctcgaag
     2821 atgtcgtaga tcttggcgtc gcggtgcatc cgctcgaccg ggaactcgcg ggtgtagccg
     2881 ttgccgccga ggatctggat cgcacgctcg gtgacccagg tcgcgacctc gccacccttg
     2941 agcttcgaca tcgagccttc ggagttcttg aacgggatct ggttgcgacc catccaggag
     3001 gcgcgccaga cgagcagccg agccgcatcg atctcgaggc acatgtcggc gagggtgaag
     3061 gcgatcgcct ggttctcgat gatcggacga ccgaactgga cgcgttcctt ggcgtactcg
     3121 agggcgtact cgtaggcggc acgggcgatg ccgatcgcct gcgagccgac gatcggccgg
     3181 gaagcctcga aggtcgccat cgctgcctgc gacttgccct tcttgccttc acgaacccgg
     3241 gcgagacgct cgtcgagctt ctccttgccg ccgagcaggc agctgcccgg cacacggcag
     3301 tcgtcgaggt gaacatcggc agtgtgcgag gcgcggatgc cgtgcttctt gaccttcgcg
     3361 ccctggctgc agcccggggt gttcggcgga atgatgaacg cagcatggcc gcgcgaaccg
     3421 agttcgcggt cgaccgaggc gatcacgacg tgaatgttgg cgatgccgcc gttggtcgcc
     3481 caggccttct ggccgttgat cacccattcg tccttggcct cgtcgtactt ggccgtggtc
     3541 cggacgttgg agacgtcgga gccggcgtcg ggctcggagg agcagaacgc ggcgatcttg
     3601 acgtcatcct cggtgccgta gcactggggg acccattcac ccatctgttc cggggtgccc
     3661 tggccgaaga tcgaggcgac ggcaaggccg gtgccctgga tcgccatgcc gagaccggca
     3721 tcgccccaga acagttcctc gttgacgatc ggcaggctga ggccggtcgg gtcggcgaag
     3781 aactgtgcgg tgccctcgaa gccgtagagg ccgatcttgg cggcctcctg cacgatctcc
     3841 caggggaact cctcgcgctc gtcccattcg tgggcggcgg ggcgcatcac attctcggca
     3901 aagccgtgga cccactcctg aatgtccttc tggtcctgat tcagctcaag gctgaacttg
     3961 ggctcagaag actcatcaac agtggtttct tctgaagttt ctgtagcagt cataccccga
     4021 atgttacacg gatcggtgta atcattcgca tgtcgtcccg tcttcgcggg acgacctcgg
     4081 aaacccgccc cttctcctag ctcaaatgat ggcgagaagg ggctctgtcg gcaaagggtc
     4141 cgccggaatg tcgttcaggc agcgcgggcc gcggtgccgt caccgaagat ctggtcgatc
     4201 tcggtcgcca gcccggggga atgcctgacc cggaagtcgg gaccgagctt gagccggtgc
     4261 tcccggtccc cttcgaccac cacgaccaca tccgattcac cctgatgctg gccgaaaacc
     4321 gatttcatgt cctccagcgt gtccagcgac atctgctccg gcaccacccg gaccacgaag
     4381 ggctcgatct tctttgcgta ggcgatcttc gccttctcga tctgggcctc gctgggattg
     4441 aaaagctcgg cgtcgcgcac cttcagcttc gagccctcct cggactggtc gacggtcccc
     4501 ctgaccatca cgacctcgtc catctggacc agttgctgct tctcggaatc ctccaggttg
     4561 aagagcatca gttcgatgtc gtgctcgatg tcgctgaggg tggcgaaggc gatgttcttc
     4621 gtttccatgt agaaatcgaa ggaccagtcg ccgccgtcgc ggccgatgcc ggaattcttg
     4681 acgccgccga agggcgtcgg caggtggcgg acgttttccg aattcaccca gatcatgccg
     4741 gcgtcgagat gatcggtgaa gcggaaggcg cgggtgatat ccgccgtcca gagatagccg
     4801 gtgaggccgt actggacgtc gttggcgagc accaaagcct ccgcctcgtc cttgaagggg
     4861 atggcggtc
//
LOCUS       DAOQFI010000036         4857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7199_length_4857_cov_2.615369, whole genome shotgun sequence.
ACCESSION   DAOQFI010000036 DAOQFI010000000
VERSION     DAOQFI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_4857_cov_2.615369"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLE93_01200"
     CDS             complement(<1..648)
                     /locus_tag="PLE93_01200"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNH85692.1"
                     /translation="MEAADLIRYRGRMIYGGRARVGNLNLAAAAVAIAAVALLALFLP
                     GNPASASTRSNLNQALNRVMKAPNGPPAISMQIVKNGHSQYYRRGVADLRTGAKPRLR
                     MRFRIASVAKAFSGAVALNLVARGKLSLHSTIGEVLPGMLPKAKQVTLGQALHHTGGL
                     PEYIKSKGFLNEVGNDPGAFVPPTRIISWVRGSGLTHRPGTKYEYSDTDNIVVGLM"
     gene            complement(630..1193)
                     /locus_tag="PLE93_01205"
     CDS             complement(630..1193)
                     /locus_tag="PLE93_01205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNH85693.1"
                     /translation="MSAGAGSPLRGFHPGAGSDAAGGPIDFTRSGVVWLEGEAVLIEP
                     TGTGKPLARLRDGRLVVGGGGRAQVLAAIDRWYRREARQRIEPLVEVEGQRLGLEAGK
                     VRIGNQRTRWGSCSTSGTVSFNWRLVLGRPEALHYVVVHELMHLRHHNHSRDFWNDLA
                     AAFPGFREEARWLRVNERRLLQWKPRI"
     gene            complement(1190..1900)
                     /locus_tag="PLE93_01210"
     CDS             complement(1190..1900)
                     /locus_tag="PLE93_01210"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNH85694.1"
                     /translation="MIIGRPHLHYRQVDSTNERARQVAAGGAPDGTLVTAAEQTAGRG
                     RQGRPWSTPAGSAVAWSVLIRRQVEIPGTLPLQVGTGVCDAVEALGASGVQMKWPNDI
                     WIDGRKCAGILVEARPQDGWAVAGVGLNLSIEDDEFPEELRGRATSLGGGVTFTEAVA
                     ALNERVGRSIDRPVEETLAAFADRDGLRGRQVSWAEGQGTAVGIDPQGNLLVESTAGC
                     IATLNAGEVHLSLPVDAA"
     gene            complement(1897..2748)
                     /locus_tag="PLE93_01215"
     CDS             complement(1897..2748)
                     /locus_tag="PLE93_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNH85695.1"
                     /translation="MSPSVSPENANRGQTRPVTFLTDFGYSDEYPGVCRLVIASIDPR
                     IKVIDLTHGIPPGDIRRGGLALAAAVRFSAPAVHLAVVDPGVGSRRRAVVLTCGEHHL
                     VGPDNGLLSLATAVLGGVEAAFDVSATPVRVGRSDTFHGRDVFSPVAAELARGAAPGE
                     LGEAVGFRELAPLELPEPLVTPEQVDAHVLYPDQYGNVILNIHSEVLDGTFFTEGDAI
                     SLETSLQKLEVPLRRTFADVEPGEPVLFTDSSGYLALAINRGSAASRFGLGPDNQLRF
                     TPVASRQ"
     gene            complement(2726..3691)
                     /locus_tag="PLE93_01220"
     CDS             complement(2726..3691)
                     /locus_tag="PLE93_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNH85696.1"
                     /translation="MRGISKASGAWSRNVAIAVAAGLGLAGLFLAPSASAGLSCRYVA
                     AGPDGPRGNRLEIKATRFEEVVALLPGKGSNIEVTDDQRMKPLACKGGKPTMVNIDRV
                     NFTADRRATGSSMYIAEAPEFGPGATPAEAGGKGITFIAKGPSLSFGIGGTDGPDLIQ
                     MGMEGKAAALDFFPDLYPDSESGDNIDAKIYAKFVNLLVRGGRGADAIAGSQIDYSST
                     WFSGPLRVPTSIYGEVGPDALTGGKSMDYLDGGPGGDFMLGAQGADQLFGGGGIDTFI
                     AGPGRDEIDALDGIGGEDLRCGRGRDLARMDLEDQDRDCESFRFP"
     gene            complement(3726..>4857)
                     /gene="efeB"
                     /locus_tag="PLE93_01225"
     CDS             complement(3726..>4857)
                     /gene="efeB"
                     /locus_tag="PLE93_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HNH85697.1"
                     /translation="PPDPEQSVVSFEGAHQAGIITPAQNRLHFAAFDLTTNRRSDLQQ
                     LLREWTAASRRMISGEMAGPVNGEPLLPPDDTGEALGLGPANLTITFGLGPGIFDRRF
                     GLASKRPPQLEPLPPLPGDELDPNISGGDLCIQACADDPQVAFHAVRNLTRIASGMAV
                     MRWCQLGFGRTAKTGAAQETPRNLMGFKDGTNNILNDDQQALDEFVWAGGEGPDWMRG
                     GSYLVARRIRMLIEAWDRTFLQEQEEVIGRAKESGAPLGEAKEDDLVDLKATGSDGTP
                     VIRQDAHIRQAAPSVNDGQKILRRGYSFTDGFDRVRGQLDAGLFFICFQRDPRKQFVP
                     IQERLGKLDGLMEYIVHDGSGLFAVPPGVSEPDGFIGQGLFS"
BASE COUNT          752 a         1649 c         1659 g          797 t
ORIGIN      
        1 catcaggccg accacgatgt tgtcggtgtc cgagtactcg tacttcgtcc ccgggcggtg
       61 ggtcagcccc gatccacgga cccaggagat gatccgggtc ggcgggacga atgccccggg
      121 atcattgccg acctcgttga gaaaaccctt cgacttgatg tattcgggca ggccgccggt
      181 gtggtgaagc gcctggccga gagtcacctg cttcgccttc ggcagcatgc cggggaggac
      241 ttcgccaatc gttgaatgca ggctgagctt gccgcgagcg accaggttca gcgcgaccgc
      301 accgctgaag gcctttgcca cgctggcgat ccggaagcgc atgcgcagcc ggggcttcgc
      361 accggtcctc aggtcggcga cgcctcgacg gtagtactgc gagtgaccgt tcttcacgat
      421 ctgcatcgag atggcggggg gtccgttcgg cgccttcatt acccggttca gggcctggtt
      481 caggttcgaa cgggtcgagg cgctggccgg gttgccgggc aaaaacaggg cgagcaaggc
      541 gaccgctgcg atcgcgacag ctgccgcagc gaggttcaaa ttcccgactc tggccctgcc
      601 cccgtagatc atgcggccac ggtaccggat cagatccgcg gcttccattg gaggagccgg
      661 cgctcattca cccgcagcca tctggcctct tccctgaatc ccgggaaggc ggcggcaagg
      721 tcgttccaga agtcgcggga atggttgtgg tggcgcaggt gcatcaactc gtgaacgacc
      781 acgtagtgga gtgcctcggg ccggccgagg accaggcgcc agttgaagga gacggtgccg
      841 gaggtcgagc aggagcccca gcgggtgcgc tggtttccga tccggacctt gccggcttcg
      901 agaccgagcc gctggccctc gacctcgacc aggggttcga tccgctgccg tgcctcgcgg
      961 cggtaccagc ggtcgattgc ggcgaggacc tgggcccggc cacccccgcc caccacaagc
     1021 cggccgtcac gcaggcgcgc gagcggcttg cctgtgccgg tcggctcgat cagaacggcc
     1081 tccccttcca gccagacgac cccggagcgg gtgaagtcga tcggcccccc ggccgcatcg
     1141 gagccggccc cggggtgaaa gccccggagc ggagaacctg ctcccgcgct cacgccgcgt
     1201 caaccggaag cgagaggtgg acctcgcccg cgttcagggt cgcgatgcag ccggcggtcg
     1261 attcaaccag caggttgccc tggggatcga ttccgacggc ggtgccctgc ccctcggccc
     1321 agctgacctg ccggccccgc agtccgtccc ggtcggcgaa agcggcgagc gtctcctcca
     1381 ccggcctgtc gatgctgcga ccgacgcgct cgttcagtgc cgccaccgct tcggtgaaag
     1441 tcacgccgcc accaagtgag gtcgcccgtc cgcgcagctc ctccgggaac tcgtcgtctt
     1501 caattgaaag attgagaccg accccggcca cggcccagcc atcctgcggt ctcgcctcga
     1561 ccaggattcc ggcgcatttg cgaccgtcta tccagatgtc attcggccac ttcatctgga
     1621 ccccggatgc accgagcgcc tcgaccgcat cgcagacccc ggtccccacc tgcagcggca
     1681 gggtgcccgg aatttcgacc tggcgtcgga tcagcacgct ccaggcgacc gccgagccgg
     1741 ccggggtcga ccatggccgc ccctgcctgc cccggcccgc ggtctgttcc gccgcggtca
     1801 ccagggtgcc gtcgggggcg ccgccggccg caacctgacg ggcccgctca ttggtcgaat
     1861 caacctgacg gtagtgaaga tgcggacgcc cgatgatcac tggcgggagg ctaccggggt
     1921 gaatctgagt tgattgtcgg ggccgagtcc gaaacggctg gcggcgctgc cgcggtttat
     1981 ggccagggcg agatagccgc tcgagtcggt gaagaggacc ggttcgccgg gctcgacatc
     2041 ggcgaaggtg cggcggagcg gcacctcgag cttctggagg ctcgtctcca gcgagattgc
     2101 gtcaccctcg gtgaagaagg tcccgtccag cacctcggaa tggatgttca ggatcacgtt
     2161 tccgtactga tccgggtaga gcacatgggc gtccacctgc tccggggtga ctagcggttc
     2221 gggcagctcg agcggggcca gctcccggaa accgacggct tccccgagtt cgccgggggc
     2281 cgcaccgcgg gccagttcgg ccgccaccgg gctgaagacg tcacggccgt ggaaggtgtc
     2341 actccggccg acccggaccg gagtggcgga gacgtcgaag gcggcctcga ccccgcccag
     2401 caccgcggtg gccagggaga gcaggccgtt gtccggaccg accagatgat gctcgccgca
     2461 ggtgagcacc acggcccggc ggcggctgcc gactccggga tcgaccacgg ccaggtgaac
     2521 cgccggggcg gagaaccgta ccgccgcagc cagggccagg ccgccgcgcc ggatgtcacc
     2581 ggggggaatg ccgtgggtca ggtcgatgac cttgatccgg ggatcgatcg aggcgatcac
     2641 cagccggcag accccggggt actcgtcgct gtacccgaag tcggtcagga aggtgaccgg
     2701 ccgcgtttgc ccccgatttg cgttctcagg ggaaacggaa ggactcacaa tcccggtcct
     2761 gatcttccag gtccatccgg gccagatccc tgccgcggcc gcaacgcaga tcctctcccc
     2821 cgattccgtc gagcgcgtcg atctcgtccc ggcccgggcc ggcgatgaag gtgtcgatgc
     2881 cgccgcctcc gaagagctgg tcggctccct gcgcgcccag catgaagtca ccgccggggc
     2941 cgccgtccag gtaatccatg ctcttgccgc cggtcagggc gtcggggcca acctcgccgt
     3001 agatcgaggt cggaactctc agcggaccgc tgaaccaggt gctcgagtaa tcgatctggc
     3061 tgccggcgat cgcgtcggct ccccgcccgc cgcgaaccag gaggttgacg aacttcgcgt
     3121 agatcttcgc gtcgatgttg tcgcccgatt cactgtcggg atagagatcg ggaaagaagt
     3181 cgagcgcggc ggccttgcct tccatgccca tctggatcag atccgggccg tcggtgccgc
     3241 cgatcccgaa gctgagcgac ggacctttgg cgatgaaggt gatgcccttg cccccggcct
     3301 cggccggggt ggccccggga ccgaactccg gcgcttcggc gatgtacatc gacgagccgg
     3361 tcgccctgcg gtccgcggtg aagttgaccc ggtcgatgtt gaccatggtt ggcttgccgc
     3421 ccttgcaggc aagcggcttc atgcgctggt catcggtgac ttcgatgttc gagcctttgc
     3481 cgggcagcag cgcgaccact tcctcgaacc tggtcgcctt gatctccagc cggttgcccc
     3541 tcgggccgtc cggtcctgcc gccacgtagc gacagctgag tccggccgag gcggaaggtg
     3601 ccagaaacag cccggccaac ccgagcccgg cggcaaccgc gatcgcgaca ttccggctcc
     3661 acgccccgct tgccttgctg attccccgca tggccggatt ctatttgccc ggccgcgcgg
     3721 ccgggctagc tgaagagtcc ctggccgatg aagccgtccg gttcactgac ccccgggggg
     3781 acggcgaaca ggccgctgcc gtcgtggacg atgtactcca tgagaccgtc cagcttgccg
     3841 aggcgttcct ggatcggcac gaactgcttg cgcggatcgc gctggaagca gatgaagaag
     3901 agaccggcat cgagctggcc gcggacccgg tcgaagccgt ccgtgaagga gtagccgcga
     3961 cgcaggatct tctggccatc gttgaccgag ggcgccgcct ggcggatgtg ggcgtcctgg
     4021 cggatcaccg gcgttccgtc ggatccggtc gccttcaggt caaccaggtc gtcctctttg
     4081 gcttcaccca ggggggcgcc actttccttg gcccggccga tcacttcctc ctgctcctgc
     4141 aggaaggtgc gatcccaggc ttcgatcagc atgcggatcc ggcgggcgac cagatagctg
     4201 ccgccgcgca tccagtccgg gccctcgccg ccggcccaga cgaactcgtc cagtgcctgc
     4261 tggtcgtcat tgaggatgtt gttggttccg tccttgaagc ccatcaggtt gcgcggggtt
     4321 tcctgggcgg cgccggtctt cgcggtgcgg ccgaagccga gctggcacca gcgcatgacc
     4381 gccatccccg aggcgatcct ggtcaggttg cggaccgcgt ggaaagcgac ctggggatcg
     4441 tcggcgcagg cctggatgca gagatcgccg ccgctgatgt tcgggtcgag ttcatcgccg
     4501 ggaagcggcg gcagaggctc aagctgtggc ggtcgctttg atgcgaggcc gaaacgccgg
     4561 tcgaagattc ccggcccgag gccgaaggtg atggtcaggt tggccggccc caggccaagc
     4621 gcttccccgg tgtcatccgg cggcaggagc ggctcgccgt tgaccgggcc tgccatctcg
     4681 cccgagatca tgcgccggct cgccgcggtc cactccctga gcaactgttg aagatcggag
     4741 cgccggttcg tggtcaagtc aaaggccgcg aagtgaagcc tgttctgggc cggggtgatg
     4801 atcccggcct gatgcgcccc ctcgaaggag accaccgact gctccgggtc gggcggg
//
LOCUS       DAOQFI010000037         4821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7286_length_4821_cov_3.375577, whole genome shotgun sequence.
ACCESSION   DAOQFI010000037 DAOQFI010000000
VERSION     DAOQFI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7286_length_4821_cov_3.375577"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLE93_01230"
     CDS             complement(<1..1316)
                     /locus_tag="PLE93_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH85698.1"
                     /translation="MDRDPGGTRLSGRERTSNGRPSAETVEAIWRIESARLVARLSRL
                     TGEVGLAEDMAQEAFIAALEVWPGSGIPRNPAAWLMTTARNRGIDRIRRDRVLTEKYG
                     QIAHEMELAGPGPGPEQAVLNEDRIGDDVLTLLFTACHPCLTTETQVALTLKAVGGLT
                     TDEIARAFLVSDSAIAQRIVRAKRTLADSGIPFEPPSTDEMGERLAAVLGVIYLIFNE
                     GYSATAGDQWMRTELSVEALRLGRIMVGLAPREPEVHGLVSLMEIQASRFPARTGPQG
                     EPVLLMDQDRSRWDRLLIRRGLKELETARSLGGNGPYVIQASIAACHARALRPEQTDW
                     QEILGYYDELAALGDSPVAELNRAVAMAMSGDPRSALDAVDRVATDSRMKGYYLLPSV
                     RGDLLERLGRSGEAAAEFERAASLTDNQAERRVLIERAERAKDFPG"
     gene            complement(1333..2163)
                     /locus_tag="PLE93_01235"
     CDS             complement(1333..2163)
                     /locus_tag="PLE93_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH85699.1"
                     /translation="MSEQQVGPKTIVITGAARGIGLATARILATRGNRVVMSDLDGEL
                     AAAEAEKIGDRAAGFGVDVTDRDAVREHLEMVEREFGPVDVMINNAGIASASPNILEQ
                     DPKITERTIDINLKGAMNGTIEAIRLMEPRRRGQVVNVASLAGIIGVKGLAAYSASKF
                     GLVGFTESIRLEFADKGIKLTCVMPGPVDTGMMDGTSSSPLVSMLAPEEMATGIADAV
                     TTGKPRVSLPRSSWLLARTISVLPPAIGIRVGHWTRIDRIYTEVDPAARAAYEERISR
                     "
     gene            complement(2213..2851)
                     /locus_tag="PLE93_01240"
     CDS             complement(2213..2851)
                     /locus_tag="PLE93_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH85700.1"
                     /translation="MGGQAEGSRTWRGQTIEERQAGRRERLIEAGIELFGTRGYANTP
                     VKAICEEAGLTERYFYEAFEDRESLLDEIYSMVLADCRRATVEAIDGAPDDFEASMRA
                     GLTAFAHEWIRDPRRARIQEIEVVGVSPEIERKRRDSIHAFAGIVVDRTLQLGGREQL
                     GALRLDVIALGLIGSVNEQLIDFVLGDLQISLDDLIENQIAAFAAVASQLLE"
     gene            complement(2851..>4821)
                     /locus_tag="PLE93_01245"
     CDS             complement(2851..>4821)
                     /locus_tag="PLE93_01245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH85701.1"
                     /translation="GADAGGEAWLRSLLEADVPDRLELSDAEVLDLLDGAAERLEASG
                     IVLDWDPDLDQSLTGKVVVGSSGRLSQSVPDVFDQNALQGFSWQLSLGGTPLSEEEMA
                     VIADTDGPIARLREKWVLVPRVMAERARHPDLKPLTAIDALSVALTGSTVVDGQRVDV
                     EAKGWVAQLREQVTGDSEQSLIEPPADLNATLREYQVEGMRWLHRMTSLGLGGCLADD
                     MGLGKTVMLISLHLHRRLNPQTAGPTLVVCPASLLGNWEREIQRFAPTEKVRRYHGPN
                     RSLDQITSGFVLTTYGTMRLDAELFEAVPWGLLVADEAQHVKNPRSAGAKALRTIPAR
                     SRVALTGTPVENNLSELWAILDWTTPGLLGSLGAFRKKWADPIEVEGSQSATRLLNRL
                     VRPFLLRRRKSDPGIAPELPPKTITDQPVELSPEQIKLYEAAVRENMLQIRTAEGINR
                     RGLVLKLLTSLKQICNHPAQFLGEEGGPLAERSGKLELLDELLDTILSEDSSVLVFTQ
                     YVAMARLLSRHLEERGIGAMLLHGGTAVRQRERMVERFQDGEVPVFLLSLKAAGTGLN
                     LTRADHVIHYDRWWNPAVEEQATDRAHRIGQLNSVQVHRLIAEGTIEDRIASIHETKR
                     ELADAIVSTGEAALTELSNEDLAELVELRNAG"
BASE COUNT          757 a         1648 c         1531 g          885 t
ORIGIN      
        1 cagcccggaa aatcttttgc ccgttcggcc cgctcgatca gtacccgccg ttccgcctga
       61 ttatcggtca gtgaagcggc gcgttcgaac tcggccgccg cctccccgct ccggccaagc
      121 cgctccagca gatcaccgcg cacgctcgga agaaggtaat agcccttcat gcgactgtcc
      181 gtcgccaccc gatccaccgc gtcgagggcc gaccgggggt ctccggacat cgccatcgca
      241 actgcccggt tgagctcggc gaccggtgaa tcccccagcg ccgccagctc gtcgtagtag
      301 ccaaggatct cctgccagtc ggtctgctcc ggcctcaacg cacgggcatg gcaggcagcg
      361 atggacgcct ggatcacgta cggcccgttc ccgccaaggc tcctcgcagt ctccagctct
      421 ttcagccccc ttctgatcag gagccggtcc cagcgtgagc ggtcctggtc catcagcaac
      481 accggttccc cttgcgggcc ggttcgcgcc gggaagcgcg aagcctgaat ctccatcagg
      541 gacacaaggc catgcacctc cggctcccgg ggagccagcc cgaccatgat ccggcccagc
      601 cggagcgcct caacggaaag ctcggtgcgc atccattgat cgccggcggt tgccgaatag
      661 ccctcgttga agatcaggta gatcacacca aggacggccg ccagccgctc gcccatctcg
      721 tcggtggacg gcggctcaaa ggggatcccg gaatcggcga gggtgcgctt ggcccggacg
      781 atgcgctggg cgatcgccga gtccgagaca aggaaagccc gggcgatctc atcggtggtg
      841 aggccgccga ccgccttcag agtcagcgcg acctgtgttt cggtggtcag gcatggatgg
      901 caggccgtga agagcagggt caggacgtca tcgccgatcc ggtcctcgtt caacaccgcc
      961 tgctccgggc cgggcccggg tccggcaagc tccatctcat gggcgatctg cccgtacttt
     1021 tcggtcagga ccctgtccct gcggatccgg tcgatccccc ggtttcttgc ggtggtcatc
     1081 agccaggctg cggggttgcg gggaatgccc gaccctggcc agacttcgag ggccgcgatg
     1141 aacgcttcct gggccatgtc ctcggccagg ccgacctccc cggtcagccg cgagagcctt
     1201 gcaacgagcc gggcggactc gatccgccag atcgcctcaa ccgtttccgc gctcgggcgg
     1261 ccattcgagg tcctttcgcg acctgaaagc cttgtgccac ccgggtcccg gtccatggac
     1321 ccacgatatt gatcagcggc tgattcgttc ctcgtatgcg gccctggcgg ccgggtcaac
     1381 ctcggtgtag atccggtcga tccgcgtcca gtgcccgacc cggatcccga tcgccggtgg
     1441 cagtaccgaa atcgtgcggg ccagcagcca gctcgatcgt ggcagcgaga cccgcggctt
     1501 gccggtggtc actgcgtcgg cgattccggt tgccatctct tcgggtgcga gcatcgaaac
     1561 cagcggcgag gaagaggtgc cgtccatcat cccggtgtct accggaccgg gcatcacgca
     1621 ggtgagtttg atccccttgt cggcgaattc cagccggatc gattccgtga atccgaccag
     1681 accgaacttg gatgcggagt aggcagcgag gcctttgact ccgataatcc cggccagcga
     1741 agccacgttc acgacctggc cccggcgcct gggttccatc aggcgaatcg cctcgatggt
     1801 cccgttcatc gcacccttca ggttgatgtc aatagtgcgc tcggtgatct tcggatcctg
     1861 ttcgaggatg ttcggcgacg cggaagcgat gccggcgttg ttgatcatca cgtcaaccgg
     1921 accgaactcc ctctcgacca tctcgaggtg ttcccggacc gcgtcccggt cggtcacgtc
     1981 gacgccgaat cctgccgcgc gatccccgat cttctctgct tctgctgcgg caagttcgcc
     2041 gtcgaggtcc gacatcacga cccggttgcc ccgcgtggcg agaatccggg cggtggcaag
     2101 gccgatgccc ctcgccgcac cggtaatcac gatcgtcttc ggtccgactt gctgttcgct
     2161 catgaggtca ttctcgcaga cctgacacga agcattgtca cctcgccggg ggctattcca
     2221 gaagctggct ggccaccgcg gcaaaggcgg cgatctggtt ctcgatcagg tcatcgaggg
     2281 aaatctgcag gtctccgagc acaaagtcga tcagctgttc gttcacggat ccgatcaatc
     2341 cgagcgcgat cacgtcaagc cgcagcgcgc cgagctgttc cctgccgccc agctgaagcg
     2401 tgcggtcgac cacgatcccg gcaaacgcat ggatcgagtc ccggcgcttg cgctcgattt
     2461 cggggctgac cccgaccacc tcgatctcct ggatgcgggc cctccgcgga tcacggatcc
     2521 attcgtgggc gaaagcggtg agcccggccc gcatcgaggc ttcgaagtca tccggggcgc
     2581 cgtcgatcgc ttccaccgtc gcccgccggc aatcggccag caccatcgag tagatctcgt
     2641 ccagcaggct ctcccggtcc tcgaaggcct cgtagaagta gcgctcggtc agcccggctt
     2701 cctcacagat cgccttgacc ggcgtgttcg cataccccct ggtcccgaac agctcgatcc
     2761 cggcttcgat cagccgctcc cgccggccgg cctggcgttc ctcgatcgtc tgcccccgcc
     2821 aggtgcggct gccttcggcc tgtccgccca tcagcccgcg ttcctcagtt cgaccagttc
     2881 ggccaggtct tcgttgctga gctcggtcag ggccgcttca ccggtgctga cgatcgcatc
     2941 ggcgagttca cgcttggtct cgtgaatcga ggcgatgcgg tcttcaatcg tgccctcggc
     3001 gatcaggcgg tggacctgga ccgagttgag ctggccgatc cggtgggcgc ggtcggtcgc
     3061 ctgctcttcc accgccgggt tccaccagcg gtcgtaatgg atcacgtggt cagcccgggt
     3121 caggttgagt ccggttccgg ccgccttcag ggaaagcagg aagaccggta cctcgccatc
     3181 ctggaagcgt tcgaccatgc gttcgcgttg cctcaccgcg gtaccgccgt ggagcagcat
     3241 cgcgccgatt ccgcgctctt cgaggtggcg tgacagcaga cgggccatcg ccacgtactg
     3301 ggtgaagacc agcaccgagc tgtcctcgct gaggatggtg tcgagcagtt cgtcgagcag
     3361 ttcgagcttg ccggatcgct cggccagcgg tcccccttct tccccgagga actgggccgg
     3421 gtgattgcag atctgcttca gcgaagtgag cagcttcagc accaggccgc ggcggttgat
     3481 tccctcggcg gtacggatct gcagcatgtt ctcgcggacc gcagcttcat agagcttgat
     3541 ctgttcgggg ctcagttcaa ccggctggtc ggtgatcgtc ttcggcggca gctccggtgc
     3601 gatgcccgga tctgacttcc gtcgcctgag caggaacggc cggaccaggc gattgagcag
     3661 acgggtggcc gattggctgc cctcgacctc gatcgggtcg gcccacttct tgcggaacgc
     3721 accgaggctg ccgagcaggc cgggagtggt ccagtccaga atcgcccaga gctcggagag
     3781 gttgttctcc accggtgtgc cggtcagggc aacgcgggag cgggccggga tcgtgcggag
     3841 cgccttggca ccggccgagc gcggattctt tacgtgctgc gcctcgtcgg cgaccagaag
     3901 cccccagggc acggcctcga acagttcggc gtcgagccgc atggtgccgt aggtggtcag
     3961 cacgaacccg ctggtgatct ggtcgaggct gcggttcggg ccgtggtagc gccgaacctt
     4021 ctcggtcggg gcgaagcgct ggatctcccg ttcccagttg ccaagcagcg aggccgggca
     4081 gacgacaagg gtcggtcccg cggtctgggg attgagccgc cggtgaaggt gaagcgagat
     4141 cagcattacg gtcttgccga ggcccatgtc atcggccagg cagccgccaa gaccgagcga
     4201 ggtcattcgg tggagccagc gcatgccttc gacctggtat tcgcgcaggg tcgcgttgag
     4261 gtcagccggc ggttcgatca gcgactgttc gctgtcgcca gtcacctgtt cacgaagctg
     4321 ggctacccag cccttggctt cgacgtccac ccgctggccg tccacgaccg tgctgccggt
     4381 cagggcgacg ctgagggcgt cgatcgcggt caggggcttc agatcgggat ggcgcgcccg
     4441 ttcggccatc acccgcggca ccagcaccca tttttcccgg agtcgggcga tcggcccgtc
     4501 ggtatcggcg atcaccgcca tttcctcctc gctcagggga gttcctccga gcgagagctg
     4561 ccaggaaaag ccctgcagcg cgttctggtc gaacacgtcg ggaacgctct ggctcaaccg
     4621 tccgctgctt cccaccacca ccttgccggt caggctctgg tccaggtccg ggtcccaatc
     4681 cagcacgatg ccggatgcct cgagcctctc ggcggcaccg tccagcaggt ccagaacctc
     4741 tgcgtcactg agttcgagcc ggtccggcac atccgcttcg agcaggctgc gcagccaggc
     4801 ctcaccaccg gcatcagcgc c
//
LOCUS       DAOQFI010000038         4805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7338_length_4805_cov_2.377684, whole genome shotgun sequence.
ACCESSION   DAOQFI010000038 DAOQFI010000000
VERSION     DAOQFI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7338_length_4805_cov_2.377684"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..789)
                     /locus_tag="PLE93_01250"
     CDS             complement(85..789)
                     /locus_tag="PLE93_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85702.1"
                     /translation="MTASSNSRSVRLACAAKGIDFGSPHAVRGGFGLVTGPGEAEYRE
                     IRLLARDPLDPAARVERALAMKRVMADASLRQPGTFAGFEPPELDVEWLRGEATKLAD
                     LRGRPVMLVFWTPISERIIPTADYIAHLIHKGEAAGLQTIVVCDGGTEPGALEEALTK
                     QPLTGARIARDRGTTLDGYFVKIGGFGMPRFLLIRRSGRVAYEGDPGFSSGVGWKPSD
                     GPTFVDQPFDALIDDR"
     gene            complement(944..2566)
                     /gene="argS"
                     /locus_tag="PLE93_01255"
     CDS             complement(944..2566)
                     /gene="argS"
                     /locus_tag="PLE93_01255"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNH85703.1"
                     /translation="MNSSADPTAALLEAVTVTARRLAGNEEISPALDPSPRPEMGDYS
                     TNAAMMLAKPLGRPPREIAAELAAAIEAELGPTLEKTEIAGPGFINLFLGDAWYREAT
                     AAMASTGAAEVPPVETPERIIVEFVSANPTGPLTAASGRHAAYGDSLSRLLRATGNGI
                     TTEYYVNDGGTQVQNFAASIAARMNGEEPPEDGYKGTYVNELAERAKAEGHDPADLEG
                     LARTGVGWMVEGIRGSLANFGVRMDDFFSERSLYEDNLVDRGIERMREAGHVFDEDGA
                     VWLKTTDFGDDKDRVLVRSTGEVTYFGADVAYHVDKIERGNDRLIDVLGADHHGYVAR
                     MAAAMEALEGGKAEFEAQIMQLIHLIEGGQRTKMSKRAGDIVTLDELTGDIGVDAARW
                     FLLQRSHNQTIDLDLDLARSQSSDNPVYYVQYAHARIQSILRKAAEEGADGGGDPLAA
                     PVDPSEKALVSKLLEFPAEVHEAAERRAPHRICAYASETAAAFHAFYRDCQVVGAEGE
                     GVEAARIQLCGVAAETIKRALELLGIGAPERM"
     gene            complement(2606..3052)
                     /locus_tag="PLE93_01260"
     CDS             complement(2606..3052)
                     /locus_tag="PLE93_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNH85704.1"
                     /translation="MKVELIAVGKIKPPFAEAEAHYLKLLKPLMPVEVTETRDDDALG
                     RQVKSALATGGNRPDPGTIVALEAGGRDMTSERWAEWLDQRRHDGQRLTLLIGGPEGL
                     PGEVSGMADHRLSLGPQTMAHQLARVVLLEQLFRASKILAGQKYHL"
     gene            complement(3042..4325)
                     /locus_tag="PLE93_01265"
     CDS             complement(3042..4325)
                     /locus_tag="PLE93_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNH85705.1"
                     /translation="MNSGSAAGRSLNGYALAGVGLGAVLAACLAVLLLAPDQGPPVPD
                     PAAAARVIADRFSPHELDRADDYRSWGRLIGLASLVVQFATLAFLAFWRGAPMRRLLG
                     WLGRRPLPGALAAGALVSLVLALTGLPFDIAAWQLGRDYGLVNQALGERLLDWLLATV
                     VTAIPAALGGLLAMALWRRLKGRFWIAASVLVAIWAVITVWLWPVVISPLFNDFDPLP
                     DGPARTGVLRLADRAGVEVGEVYEVDASRRSSTLNAYVNGIGSTKQVVIYDNAIRTLN
                     RGEFTALVGHELGHVKAADLRRGLLFALLVIPLGVLFVQLATAAALRRNGDDPHSPAL
                     IPVIALWMALAVFALQIPGNALSRQVEAKADRYSIALTGDPQGLVDLQVRLAKANLSD
                     VDPPAFWQFMFGTHPSTFDRIAIAEGAKGGSRDEG"
     gene            4359..>4805
                     /locus_tag="PLE93_01270"
     CDS             4359..>4805
                     /locus_tag="PLE93_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNH85706.1"
                     /translation="MKVAVISDIHANRHALDAVLADIDESGIEEIWFLGDAVGYGAFP
                     GECVLTLKDRCSVFLLGNHDLAALREIDIGTFSPSAAISARWTREHMEEDAFEVLRGL
                     GGTSAEREGIGLFHASPRDPIWEYVVDSDLAEDNLDFQEQRVALIGH"
BASE COUNT          796 a         1633 c         1612 g          764 t
ORIGIN      
        1 gcggtcgcgg accgtggtcg tccgcaccgt cggtttctcc gacgccatcg ctccccgcgc
       61 gtcccgcaac gggacgcggc gcgctcagcg atcgtcgatc agagcgtcga agggctggtc
      121 gacgaaggtc ggaccgtcgc tgggcttcca gcccacgccg ctgctgaagc ccgggtcgcc
      181 ctcgtaggcg acgcgccccg agcggcggat caagaggaag cgcggcatgc cgaagccgcc
      241 gatcttcacg aagtagccat cgagcgtggt gccgcggtcg cgcgcgatgc gggctccggt
      301 caacggttgc ttggtcagcg cctcctccag cgcaccgggt tcggtgccgc cgtcgcacac
      361 cacgatggtc tgcagtcccg cggcttctcc cttgtggatc aggtgcgcga tgtagtcggc
      421 ggtcgggatg atccgctcgg agatgggggt ccagaacacc agcatcaccg ggcggccgcg
      481 cagatcggcg agcttggtgg cttcgccgcg caaccattcg acgtcgagct ccggcggctc
      541 gaagccggcg aaggtgcccg gctggcgcag cgacgcgtcg gccatcaccc gcttcatcgc
      601 cagcgcccgt tcgacgcgcg ccgccggatc gagcggatcg cgggcgagca ggcggatctc
      661 gcggtactcc gcttcgcccg gtccggtgac gagcccgaag ccaccgcgca ccgcgtgcgg
      721 cgagccgaag tcgatgccct tcgccgcgca ggccagccgc acgctgcgcg agtttgacga
      781 cgccgtcacc ggaaaagctt cggtccagcg ttccggcggt cggggccttg gcagcggccg
      841 gcccggccaa cagcatgagc ccgaaaagcg ccgtgaaaag cagcctcttc atgacctcaa
      901 aaacaccggg taacacggaa cgattcggtt ccgtgcagca ccctcacatc cgctcgggag
      961 cgccgattcc gagcagttcc agtgcgcgtt tgatcgtttc ggcagcgacg ccgcagagct
     1021 ggatccgggc ggcctcgacc ccctctcctt cagcccccac aacctggcag tcgcggtaga
     1081 aggcgtggaa ggcggcggcg gtctcgctgg cataggcgca gatccggtgc ggggcgcgcc
     1141 tttctgccgc ttcgtgaacc tcggccggga actcgagcag cttcgaaacg agcgctttct
     1201 ccgaaggatc gaccggagcg gcgagcggat caccaccccc gtcggcacct tcctcggccg
     1261 ccttgcggag gatgctctgg atccgggcgt gggcgtactg gacgtaatag accgggttgt
     1321 ctgacgactg gctgcgggcc aggtcgagat cgagatcaat ggtctggttg tgactccgct
     1381 gaagcaggaa ccagcgggcg gcgtcgacgc cgatgtcccc ggtcagctcg tccagggtga
     1441 cgatgtcccc tgcccgcttg ctcatcttgg tccgctggcc gccctcgatc aggtggatca
     1501 actgcatgat ctgggcttcg aactcggcct tgccgccttc gagtgcttcc attgccgcgg
     1561 ccatccgtgc cacgtaaccg tggtggtcgg ctccgagcac gtcgatcagc cggtcgttgc
     1621 cccgctcgat cttgtcgacg tggtaggcga cgtcggcccc gaagtaggta acctcgcccg
     1681 tggacctgac caggacccgg tccttgtcgt cgccaaagtc ggtggtcttc agccagaccg
     1741 cgccgtcctc gtcgaagaca tggcccgctt cgcgcatccg ctcgatgccg cggtcaacca
     1801 ggttgtcttc atagagcgag cgctcggaga agaaatcgtc catccggacc ccgaagttcg
     1861 ccagcgaacc gcggatgccc tcgaccatcc acccgacccc ggtccgggcc aggccttcga
     1921 gatcggccgg gtcgtgacct tcggccttcg cccgctcggc cagctcgttc acgtaggtgc
     1981 ccttgtagcc gtcctcgggg ggctcctccc cgttcatgcg ggcggcgatc gaggcggcga
     2041 agttctggac ctgggtgcca ccgtcgttga cgtagtactc ggttgtgatc ccgttgccgg
     2101 tggctcgaag caggcgtgaa agcgagtcgc cgtaggcagc gtgccggccc gaagcggcgg
     2161 tcagggggcc ggtcggattg gcggagacga actcgacgat gatccgttcc ggggtttcga
     2221 ccggcggcac ttcggccgct ccggtcgagg ccatggcggc ggtagcttcg cggtaccagg
     2281 cgtcaccgag gaagaggttg atgaaaccgg ggccggcaat ctcggtcttc tcgagagtcg
     2341 ggcccagctc ggcctcgatc gcggcggcca gctccgcggc gatctcgcga ggtggccggc
     2401 ccagtggctt ggccagcatc atcgccgcgt tggtcgagta gtcgcccatt tcggggcggg
     2461 gcgacgggtc aagggccggg ctgatttctt cattgccggc cagccgccgg gccgtgacgg
     2521 taacggcctc aagcagagcg gcggtgggat cggcggaact gttcacgccg gcgattctat
     2581 ttgcgggccc ggccggccgc tgtcactaga ggtggtactt ctggccggcg aggatcttgc
     2641 tggcccggaa gagctgctcc agcaggacga cgcgggcgag ctgatgggcc atggtctgcg
     2701 gaccgagcga gagccggtgg tcggccatcc ccgagacctc ccccggcaaa ccttccggac
     2761 cgccgatcag caaggtcagc ctctgaccgt cgtggcggcg ctggtccagc cattcggccc
     2821 agcgctcgga ggtcatgtcc cgtccgccgg cctcaagcgc gacgatcgtg cccggatcgg
     2881 gccggtttcc gccggtcgcc agggcggact tcacctgccg ccccaaggcg tcgtcgtccc
     2941 tggtttcggt gacctcgacc ggcatcagcg gcttcaacag cttcaggtaa tgggcctcgg
     3001 cctccgcaaa cgggggcttg atcttcccca ccgcgatcag ttcaaccttc atcccggctg
     3061 cctcccttcg caccctcggc gatcgcgatc cgatcgaagg tcgagggatg ggtaccgaac
     3121 atgaattgcc agaaggccgg cggatcgaca tccgacaggt tggccttggc gagccgcacc
     3181 tggaggtcga ccagaccctg gggatcgccg gtcagggcga tcgagtatcg gtccgccttc
     3241 gcctcgacct ggcgggacag cgcgttgccg ggaatctgga gcgcaaagac cgccagggcc
     3301 atccagaggg cgatcaccgg gatcagcgcc gggctgtggg ggtcgtcgcc attgcggcgc
     3361 agggccgccg cggtagcaag ctggacgaag agcaccccca gcggaatgac cagcagggcg
     3421 aagagcagcc cgcgcctcag gtccgccgcc ttcacatgcc cgagctcatg cccgaccagg
     3481 gccgtgaact cgccccggtt cagggtccgg atcgcgttgt catagatgac cacctgtttg
     3541 gtcgagccga ttccgttcac gtacgcattc aaggtagagg aacggcgact tgcgtcaacc
     3601 tcgtagacct cccccacctc caccccggcc cggtccgcca gccgaagcac cccggttcgc
     3661 gccgggccgt cgggaagcgg gtcgaaatcg ttgaacaacg gcgagatgac aaccggccag
     3721 agccagacgg tgatcaccgc ccagatcgcg accagaaccg acgccgcgat ccagaacctg
     3781 cccttcagcc ttcgccaaag tgccatcgcc agcagtccgc ccagcgcagc aggaattgcc
     3841 gtgaccacgg tggccagcag ccagtcgagc aagcgctcac cgagagcctg attgaccagt
     3901 ccgtagtccc gcccgagttg ccaggcggcg atgtcaaagg gcagaccggt caatgccaga
     3961 acaagggaaa cgagggcgcc ggcggccagc gcgccgggca gcggccggcg gccgagccag
     4021 cccagcagcc gccgcatcgg ggcaccacgc cagaaagcaa gaaaggcgag ggtggcgaac
     4081 tggacgacca gggacgcgag gccgatcagg cgaccccagg aacggtaatc gtcggcccgg
     4141 tccagttcat gcgggctgaa ccggtcggcg atcacccggg ctgcggcggc cggatcggga
     4201 accggaggcc cctgatcggg agcgagcagg agcacagcca gacaggccgc cagcaccgcg
     4261 cccaacccga ccccggccag ggcgtagccg ttcaatgacc gacccgcggc cgaacccgaa
     4321 ttcattgctc aatacgatat tcggcgggcc gcctgaccat gaaagtcgcc gtcatctcgg
     4381 acatccacgc caaccggcac gcactggacg ccgtgctcgc cgacatcgat gaaagcggga
     4441 tcgaggagat ctggtttctt ggtgatgcgg ttggctacgg cgcctttccc ggcgaatgcg
     4501 tactgaccct gaaggatcgc tgttcggtct tcctgctcgg aaatcacgac ctggccgcgc
     4561 tccgggagat cgacatcggc accttcagcc cctcggctgc gatatctgcc cgctggaccc
     4621 gggaacacat ggaggaggac gccttcgagg ttcttcgtgg cctgggcgga acctcggccg
     4681 agcgggaggg gatcggcctt tttcacgcct cgccgcgaga tccgatctgg gaatacgtcg
     4741 ttgacagcga cctggccgaa gacaatctcg acttccagga acagcgggtc gccctgatcg
     4801 gccat
//
LOCUS       DAOQFI010000039         4785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7390_length_4785_cov_4.492389, whole genome shotgun sequence.
ACCESSION   DAOQFI010000039 DAOQFI010000000
VERSION     DAOQFI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7390_length_4785_cov_4.492389"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLE93_01275"
     CDS             <1..950
                     /locus_tag="PLE93_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH85707.1"
                     /translation="GFGDGGIKARLEADVILVAGSRIGNLDLPYDKYWGDPENQSLIQ
                     IDTDPSHLGVTRPLAIGMVADAADALGGITAELKRRDKTPSAEATAALAEAREADAAW
                     QAVQASTADDWEGEGIHPARAIEVIGETFGDQAIYTVDGGLTSVWAYAGLPPTPPRSY
                     HSILELGMLGTGIPSAIGSKIGCPDRDVVCVTGDGAAGFNIMEMQSAARDSIPVTVIV
                     FAEGSWTMEEPNELALFNRTFGTEQGETRWDLVAEGLGCHGEFVDQLGDLGEALVRCR
                     DSGRPSVVCLKTNRDANRVIPPAMAARWMEVQAGPIPGV"
     gene            1338..3086
                     /locus_tag="PLE93_01280"
     CDS             1338..3086
                     /locus_tag="PLE93_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNH85708.1"
                     /translation="MDARDGMDLKYSVLLPPGDGPFPAIMNYSGYDPGSIGGTAYQAG
                     NTTMDPELDAKLLKAGYAVVGVNMRGTGCSDGNFSLFANEWGTDGYDAVEWTADQPWS
                     NGKVGMANWSYAGLSQILTAVNNPPHLKAIAPGMAVTDQWRDVASPGGVTNWLFPYGW
                     GLFIQQRWNAAKTSAEVEGDTKCIDNIAAHNAGFNAVSPYREGVERPHVDNKPGRTNH
                     VWRQVHKIKAAVISMVAWQDEATGPRAGYYQNRLDPKRSYLVGTNGPHDIYMADRWQA
                     MLIPFFDRYVKGKKNGFEKKPHVRLWFDTKTPDGSSTKSGNLRKSTPGTVLTQKSLPI
                     KVKPLRLSLRSSGSLTTNKPRKPEPASSYDFPTVSPNVNPDYAEPGDTDWQEDPADPN
                     GTVAFTTAPLQRTLTFQGPASVDLWVSATTPDADIQATITEVRPDGKEVYVNRGWLRL
                     SERALDKKLSTPTLPFLKRTKGAVQMLKDSKPVYGRVEIMQFAHIFRKGTSIRVLLDT
                     PSTTGGYGFEGPRDDTKIKVFHGPNRNSKLVLGLMRVGGIKTPKPACDTVLRQPCREN
                     TFSIPTGKGPDGSTAR"
     gene            3351..4567
                     /locus_tag="PLE93_01285"
     CDS             join(3351..3639,3639..4567)
                     /locus_tag="PLE93_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612098.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNH85709.1"
                     /translation="MSRSKRYPREFRERSVALVLEHQGEHRSQWACIQSVAAKSGMTP
                     ETLRRWVRQAEVDSGGRAGVSTDERKRIRELERENRELRRANEILKAASAFFGAGARP
                     ASTEEMTAFIEEHREKFGIEPICQIMDFAPSTYYAALKRRENPSARDLRDQELIPEIR
                     RVHRENLGVYGARKTWLQLKREGIMVPRCQVERLMREEGLQGVVRGRKAPRTTFPGGQ
                     AKRALDLVDRKFEAQAPNRLWVADFTYVFTWEKLCFAAFVIDVFSRRIVGWRVADSMR
                     TELVLDAFEMALWSRGHEDLPVGEGLVVHSDAGSQYTSFAFTRRLIDAGVDASIGSVG
                     DAYDNALAESTIGLYKTELINKDGPWRTRSDVELATLTWVDWFNNRRLHGACGDLPPI
                     EFEQQHQAASITQ"
     misc_feature    3629..3745
                     /locus_tag="PLE93_01285"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT         1014 a         1483 c         1474 g          814 t
ORIGIN      
        1 tcggcttcgg ggacggcggc atcaaggccc ggctggaggc cgacgtgatc ctggtcgccg
       61 ggtcgcggat cggcaatctc gacctcccct acgacaagta ctggggcgac cccgagaacc
      121 aaagcctgat ccagatcgat accgacccca gccatctcgg tgtaacccgg cccctggcga
      181 tcggcatggt cgccgacgcc gccgacgcgc tgggcgggat cacagccgaa ctgaaaagac
      241 gggacaagac tccctcggcc gaagcgaccg cggccctggc cgaggcccgg gaagctgacg
      301 cggcctggca ggcagttcag gcctccaccg cggatgactg ggaaggcgag gggatccacc
      361 cggcccgggc gatcgaggtg atcggcgaga cctttggcga ccaggcgatc tacacggtgg
      421 acggcggcct gacctcggtc tgggcctacg ccggcctgcc gccgaccccg ccccgttcgt
      481 accacagcat cctcgagctg ggcatgctcg gaaccggcat tccttccgcg atcggatcga
      541 agatcggctg tcccgaccgg gatgtcgtct gcgtgaccgg ggacggcgcc gccgggttca
      601 acatcatgga aatgcagtca gccgcgcgcg actcgatccc ggtcaccgtg atcgtcttcg
      661 ccgagggctc ctggaccatg gaggaaccaa acgaactcgc cctcttcaac cggaccttcg
      721 gtaccgagca gggcgagacc cgatgggatc tggtcgccga ggggctcgga tgccacggcg
      781 aattcgtgga tcaactcgga gacctgggcg aggccctggt ccggtgccgt gattccggcc
      841 ggccttcggt tgtctgcctg aagacgaacc gggatgcgaa ccgggtcatc cccccggcga
      901 tggccgcccg ctggatggag gtccaggcgg gcccgatccc gggggtctga acccggccca
      961 tatcccgaag cgcgccctcc ggatactggg cgtcgcacca gcctgctgcg ggcggaatac
     1021 ctcagttcaa gtggacggcc aagaccaggt acaggatcaa actacctttc aggcaacagc
     1081 gttgtgtatg gttagacaac attgttgtct aggaggagga gtgcgttacc gtggtgcaaa
     1141 ctgaagctgg actcctggtc ctgaagcgga cttcggcttg gtcagtcgca attgctttgg
     1201 cagtcgtcgg actgctggta ttcagctcgc tggcccaagc ggcaaacccg ttgcctgaca
     1261 gtcaacgggc cgaccttccc aagcccggta attacctgcc cgtcaatggg gtctgggcgg
     1321 agcgcttgac cggatacatg gatgcccgag acggtatgga cctgaaatac agcgtccttc
     1381 ttccacccgg cgacggaccg ttcccggcga tcatgaatta ctcgggctat gaccccggaa
     1441 gcatcggtgg caccgcctat caagctggca atacaaccat ggaccctgaa cttgacgcga
     1501 agctgctcaa agctgggtac gcggtggtcg gcgtgaatat gcgcggaaca ggatgctcgg
     1561 acggcaactt cagcttgttc gcgaacgaat ggggcacgga tggctacgac gccgtcgagt
     1621 ggacggccga tcagccgtgg tcgaacggca aagtcggaat ggcgaattgg tcgtatgcgg
     1681 ggctaagtca gatcttgact gcggtgaaca atcctccgca cctgaaggcg atcgctcccg
     1741 gcatggccgt gaccgatcag tggcgcgacg tcgccagccc gggcggtgtg accaactggc
     1801 tattccccta cggctggggt ctcttcatcc agcaaaggtg gaacgccgcc aagaccagcg
     1861 cagaagttga aggcgacacc aagtgcattg acaacatcgc cgcccacaat gccggtttca
     1921 acgccgtcag cccctatcga gagggtgtcg agcgtccaca tgtcgacaac aaacccggtc
     1981 ggacaaatca cgtctggcgt caggtccaca agatcaaggc cgcggtcatc tcgatggttg
     2041 cctggcagga cgaagcgacc ggacctcgag cgggctacta ccagaaccgc ctcgatccga
     2101 agaggtccta cctcgtcggc accaacggcc cgcacgacat ctacatggca gaccgctggc
     2161 aggcgatgct gattccgttc ttcgatcgct acgtaaaggg caagaagaac ggcttcgaga
     2221 agaagccgca cgtccgcctg tggtttgaca cgaagacccc ggatggatct tctaccaaga
     2281 gtggcaacct ccgaaagtcg acccccggaa ctgtcctgac ccagaagagc ctgccgatca
     2341 aggtcaagcc gcttaggctg tcacttcgat caagtggaag cctgacgacg aacaagccga
     2401 ggaagccgga accggcctcg tcgtatgact tcccgacggt gagcccgaac gtcaatcctg
     2461 actacgccga acccggcgac acggattggc aagaggatcc tgctgacccg aacggaacgg
     2521 tcgctttcac caccgctccg ctccagcgga ctctgacgtt ccagggcccg gccagcgttg
     2581 acctctgggt gtcggcaacg actccggacg ccgacattca ggccacgatc accgaggtcc
     2641 ggcctgacgg caaggaggtc tacgtcaacc gcggttggct gcgcctctcc gaaagggcac
     2701 tcgacaaaaa gctctcgacc cccacccttc cgttcctgaa gaggaccaag ggcgcggttc
     2761 agatgctgaa ggactctaag cccgtctacg gacgagtgga gatcatgcag ttcgcccaca
     2821 tcttccgtaa gggcacgagc atcagggttc tccttgacac gccgagcacc accggtggat
     2881 acggattcga aggcccgcgg gacgacacca agatcaaggt gttccacgga ccgaaccgga
     2941 actccaagct cgtgcttgga ctgatgaggg tcggcggcat caagacaccg aagcctgcct
     3001 gtgacaccgt gctgcggcag ccttgccgtg agaacacatt ctcgattccc acgggcaagg
     3061 ggcctgacgg ttctactgcc aggtaagagc ggcctgtcgg cagcctacgc ggagaaacga
     3121 ccgcgtaggc tgccgttagt gccacctgaa atcaacgttc aagagcgcct gtcggcgatt
     3181 gcagaagcga cccttcaact cgttcgtgac gagggaaccc gtggagttac tgttcgagct
     3241 gtcgcgaaaa aactcggtgg ctcaacttcg cttgtcacca agtacgtccc gtcgcgaatt
     3301 gaaccgcccc gggtttgttg gaggccaatt gctttgagag gatcggtcaa atgtctaggt
     3361 cgaagaggta tccgcgggag ttccgggagc gttctgttgc cctggtgctc gagcaccagg
     3421 gcgagcatcg ctcgcagtgg gcgtgtattc agtcggtcgc ggcgaagtcg gggatgaccc
     3481 cggagacgtt gaggcgttgg gtcaggcagg cggaggttga ttccggtggc cgggccgggg
     3541 tcagcaccga tgaacgaaaa cgcattcgtg aactcgaaag ggaaaaccgg gagctcagga
     3601 gagcaaacga gatcctgaag gccgcttcgg cttttttcgc gcgggagctc gacccgcatc
     3661 caccgaagag atgaccgcgt tcatcgagga gcatcgcgag aagttcggga tcgagccgat
     3721 ctgccagatc atggatttcg ccccgtccac ctactatgcg gcactcaaac gaagagagaa
     3781 cccgtcagcg agagatcttc gtgaccagga gttgatcccg gagatccgga gggtccaccg
     3841 ggaaaacctt ggtgtctacg gggcaaggaa aacctggctt cagctgaaac gggagggcat
     3901 catggtgccg cgctgccagg tggaacgcct gatgcgcgag gaaggtcttc aaggcgtggt
     3961 caggggccgg aaagccccga gaacaacctt tcctggaggg caggctaaac gggccctgga
     4021 tctggttgac cggaagttcg aagcccaggc accgaaccgg ctctgggtcg ctgatttcac
     4081 ctacgtgttc acgtgggaga agctttgctt tgcggcgttc gtgatcgacg tgttttcccg
     4141 ccggatcgtt ggctggaggg tcgctgactc aatgcgaacc gaactggttc tcgatgcgtt
     4201 cgagatggcg ctctggtcca gaggccacga agaccttccg gtcggcgagg gactcgtggt
     4261 tcactccgac gccggtagcc aatacacatc gtttgcgttc actcgccggc tcatcgatgc
     4321 cggcgtcgac gcttcaatcg gatcggtcgg ggacgcctac gacaacgcgc tggcggaatc
     4381 caccatcggt ctctacaaaa ccgagctgat caacaaggac ggaccctggc ggacccgctc
     4441 agacgttgaa ctggcaaccc tgacctgggt tgactggttc aacaaccgca ggctccatgg
     4501 agcctgcgga gacctgccac ccatagagtt cgaacagcaa caccaagcag catcaatcac
     4561 ccaatgaaaa ccgtctccac aaaacccggg gcggttcaag ccccgccaag accccggcca
     4621 aggtcaataa tccgaccacg atcaaaccac gtgggaactc gtgagcagca atctccgccg
     4681 cgacaaagac agccagaacc ccaaggatca gcgacccact cgccgccaac cgctcccgac
     4741 ctccaaccga atccctcacc cggcaaacct acctttactt ggacg
//
LOCUS       DAOQFI010000040         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7494_length_4748_cov_3.426593, whole genome shotgun sequence.
ACCESSION   DAOQFI010000040 DAOQFI010000000
VERSION     DAOQFI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7494_length_4748_cov_3.426593"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLE93_01290"
     CDS             <1..469
                     /locus_tag="PLE93_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH85710.1"
                     /translation="VEVVDIGSAVKDVEAAVEVPVGLELRTDVEPDLAAKGDPTLLRQ
                     VLIGLVTNACKNTPAPGSVTIRGRRVGDAEMLLEVIDTGTGIPQAEIERVFERFYRRS
                     ETRRQEGFGLGLAIARRMADVMGGEMGANSVEGEGSTFWARLPLIDQSKTPIA"
     gene            466..1188
                     /locus_tag="PLE93_01295"
     CDS             466..1188
                     /locus_tag="PLE93_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH85711.1"
                     /translation="MNTNPSMNGAAGRILIADDEPSVRDSVGYALAQEGFEVTAAEDG
                     TDAEERLAGEFDFDLLILDIMMPGKSGLDICREVRSRSTVPIIMLTAKDAEVDKVVGL
                     EVGADDYVTKPFSLRELLGRVRAQLRRRELDRAPAPEQTRISAGPVTIDLARHVVTVN
                     GKTVSLTRSEFQLLRLLAARPGEVFSRAKIMEELWQTEFDGDERACDVHISNLRQKVE
                     TDPQRPELVLTVRGIGYKFADS"
     gene            1316..2551
                     /locus_tag="PLE93_01300"
     CDS             1316..2551
                     /locus_tag="PLE93_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNH85712.1"
                     /translation="MATLVTELDLPGIDPADPELKGDRWHVAANGLLDSGKWLAQGPL
                     SLVVIDREAGEFFLRSRSAEFPGRLIADLFGIKDGPLRDQVDANIINRSGDDHRRLRS
                     LVNPALTPRAANRWRPVMREILEGLWEELETKEFDFVSRFSRPYPSQTIARVMGAPAV
                     DASRLHDWSEWIQRQFDPIALSDPETVETVQDKVAEFYAWVDPLIEERRGTPADDLIT
                     TLIRTEEEGEKLSDQELRNLVLNILVGGVDTTQSQLSQAIRLLAAAPDQWGLLREDPE
                     KMVPQAVSEVLRFEPVTAFTARQLVEEVEYRDVTFPAGTLLVVCSFTGNRDPEAFGDP
                     RDFDIAAERGKTRPLTFGAGIHYCVGSNIARAELEEGLRFLAEKVETIELNGLTELQA
                     VSGIYGIDRMDVRIQPVSG"
     gene            2561..3961
                     /gene="wecB"
                     /locus_tag="PLE93_01305"
     CDS             2561..3961
                     /gene="wecB"
                     /locus_tag="PLE93_01305"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNH85713.1"
                     /translation="MTEQKGTDKPVPVKILVVVGTRPEAIKLVPMILALKENEMFIPQ
                     IVSTGQHQEMVKEVFDLAGIEIDFNLWVGGARNELNDRVREVMARLDDFVRVEYGADG
                     TVKQDEAAAGEYPMAIMVHGDTTSAFAAALAAFHLRIPVIHVEAGLRTQSNLTPFPEE
                     LNRQLITCIAAFNLAPTSHNLENLVRENVPVSQIFITGNTGIDSLQWAAGLEVEFSDP
                     IVAEICSSDRRVVTITAHRRENWGAGLDGIVAGVRLLAIEHPNTYFVVPQHPNPAVRQ
                     QLANLGDLPNVCLTDALSYPEFARLMARSYLIITDSGGIQEEAPSLGKPVLVCRESTE
                     RGEGVEAGTLRLVGTDPARIQAEADLLLKSEAAYQAMSEAENPYGDGRASERIVAALT
                     NLYVGGKMPEPFRSTYSRNAVIKASGYTLPGEASGRLDPALVPESERHHEEAVVDPVG
                     NLWAEFGTITEKWFRT"
     gene            3946..>4748
                     /locus_tag="PLE93_01310"
     CDS             3946..>4748
                     /locus_tag="PLE93_01310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH85714.1"
                     /translation="MVPHLIFAEWYPFLGLDPFFEVLFLITFLIVVAMFFWTVVLFFR
                     GIKSDRHLARQEEVNPELFEWIFLVPALNEEVTIADSVSRLEAIEVARKRIVVINDGS
                     DDRTAEILAERTDPDLYVVERKAPQARQGKAAALNYAFDEVIKRFKLDPETTIFCIVD
                     ADGRIAPDSLSFVAKHFVDPKVGGVQTLVRIYNRHRILTWFQDIEFSIYGHLFQAGRN
                     KWGTAGMGGNGQYNRMAALQSIDDRDPGPAEEATVENDPEPEVAVEPASR"
BASE COUNT          851 a         1564 c         1548 g          785 t
ORIGIN      
        1 ggtcgaggtg gttgacatcg gctccgccgt gaaggacgtc gaagcggcgg tcgaggttcc
       61 ggtcggtctc gaactgagaa ccgacgtcga gccggatctg gctgccaagg gtgacccgac
      121 cctgctcagg caggtgctga tcgggctggt caccaacgcc tgcaagaaca ctccggcccc
      181 cggttctgtg accatccgcg gtcgccgggt cggggacgcc gagatgctgc tcgaggtgat
      241 tgacaccggt accggcatcc cccaggcgga gatcgaaagg gtcttcgaac gcttctaccg
      301 gcgctccgaa acaaggcgcc aggagggctt cggactgggt ctcgcgatcg cccgcaggat
      361 ggccgatgtg atgggcggag agatgggagc caattccgta gagggcgagg gcagtacctt
      421 ttgggctcgc cttcctctga tagaccagtc gaagacacca atcgcatgaa caccaatcca
      481 tccatgaacg gcgccgccgg gcgcatcctg atcgccgacg acgaaccttc ggttcgcgac
      541 tcggtcggct acgcgctggc ccaggagggg ttcgaggtga ccgccgccga ggatggcacc
      601 gatgccgaag agcgcctggc cggcgagttc gacttcgacc tgctgatcct cgacatcatg
      661 atgccgggca agtccggcct cgacatctgc cgcgaggtcc gttcccgttc cacggtgccg
      721 atcatcatgc tcaccgccaa ggacgccgag gtcgacaagg tggttggcct cgaggtcggg
      781 gccgatgact atgtgaccaa gcctttctcg ctgcgggagc tgctcggccg ggtccgggcc
      841 cagctccggc gccgcgagct tgatcgcgcc cccgcccccg agcagacccg gatctccgcc
      901 ggaccggtca cgatcgacct ggcccgccac gtggtgaccg tcaacggcaa gacggtcagc
      961 ctgacccgtt ccgagttcca gctgctccgc ctgctcgccg cccgtcccgg tgaagtgttc
     1021 agccgggcca agatcatgga agagctctgg cagaccgaat tcgacggaga cgaacgcgcc
     1081 tgcgacgttc acatctccaa cctccgccag aaggtcgaaa ccgaccccca gcgccccgaa
     1141 ctggtcctca cagtccgcgg aatcggctac aagttcgccg attcctgacc gcagtcgagc
     1201 cttcggcctt cctgtaaccc tggcgccggt tggcgtgacc tgcttcgagc agcggccccg
     1261 ccgacccggg acatccataa gcggatgggt cggctacagg ggatagcctt cgggaatggc
     1321 gaccctggtc accgaactgg acctgcccgg gattgatccg gctgatcccg aattgaaggg
     1381 ggaccggtgg catgttgcgg cgaacggact gctggactcg ggcaagtggc tggcgcaggg
     1441 gccgctttcg ctggtggtca ttgaccggga ggcgggggag ttctttctgc gttcccggtc
     1501 ggccgaattc cccggccggc tcatcgccga cctgttcggg atcaaggacg ggccactcag
     1561 ggaccaggtt gacgccaaca tcatcaaccg gagcggtgac gaccaccggc ggctgaggag
     1621 cctggtcaat cctgccctga ccccgcgggc ggccaaccgg tggcgaccgg tcatgcggga
     1681 gatcctcgaa gggctctggg aggaactgga aaccaaggag ttcgacttcg tctcgcgttt
     1741 cagccggccc tatccctccc agaccatcgc ccgggtgatg ggcgcaccgg cggtcgatgc
     1801 ctcccgactt cacgactggt ccgaatggat ccagcgtcag ttcgacccga tcgccctctc
     1861 cgacccggag accgtcgaga cggttcagga caaggtcgcc gagttctacg cctgggtcga
     1921 tccgctgatc gaggagcgtc gcggcacccc ggccgatgac ctgatcacca ccctgatcag
     1981 gaccgaggag gaaggggaga agctctccga tcaggaactc cggaacctgg tcctgaacat
     2041 tctggtcggc ggggtcgaca ccacccagag ccagctttcc caggcgatcc ggctgctggc
     2101 cgcagcaccc gatcagtggg ggctgctccg cgaggacccg gaaaagatgg tcccgcaggc
     2161 ggtcagcgag gtactgcgtt tcgaaccggt cacggccttc accgcccgtc agctggttga
     2221 ggaagtcgag taccgggacg tgaccttccc ggccggcacc ctgctggtgg tctgttcctt
     2281 caccgggaac cgggacccgg aggccttcgg ggatccgcgg gactttgaca tcgccgcgga
     2341 gcgcggcaag acccggccgc tcaccttcgg cgccggcatt cactactgcg tcggctcgaa
     2401 catcgcccgg gccgaactcg aggagggcct gcggttcctc gcggagaagg tcgaaacgat
     2461 cgaactgaac ggcctgaccg agctgcaggc ggtcagcggg atttatggca tagaccgcat
     2521 ggatgtgcgg attcagcctg tatccgggta ggcttcgcga atgaccgagc agaagggaac
     2581 cgacaagccg gttccggtga agatcctcgt ggtcgtcggc acccggccgg aggcgatcaa
     2641 gctggttccg atgatcctcg ctctcaagga gaacgagatg ttcatcccgc agatcgtttc
     2701 caccggtcag caccaggaaa tggtcaagga agtcttcgat ctggccggaa tcgagatcga
     2761 cttcaacctc tgggtcggcg gggcgcgcaa cgaactgaat gaccgggtcc gggaggtgat
     2821 ggcccggctg gacgatttcg tccgggtcga gtacggggcc gacggcacgg tcaaacagga
     2881 cgaggccgca gccggtgaat acccgatggc gatcatggtc cacggcgaca cgacctcggc
     2941 cttcgctgcc gcgctggccg ccttccacct gaggatcccg gtgatccacg tcgaagcggg
     3001 cctcaggacc cagtcgaacc tgaccccgtt cccggaggaa ctgaaccggc agctgatcac
     3061 ctgcatcgct gccttcaacc tcgcaccgac ctcccacaac ctcgagaacc tggtccggga
     3121 aaacgtgccg gtcagccaga tcttcatcac cggcaacacc ggaatcgact cgctgcagtg
     3181 ggcggccggg ctcgaggtcg agttctccga tccgatcgtg gccgaaatct gctccagtga
     3241 ccggcgcgtc gtcaccatca cggcccaccg gcgcgagaac tggggcgccg ggctcgacgg
     3301 tatcgtcgcc ggtgtccggc tgctcgcgat cgagcatccg aacacctact tcgtcgtccc
     3361 gcagcatccg aatccggcgg ttcggcagca gttggcgaac cttggcgacc tgcccaacgt
     3421 ctgcctgacc gacgcgcttt cctaccccga gttcgcccgg ctgatggccc gctcctatct
     3481 gatcatcacc gactcgggcg ggattcagga ggaggcacca tcgctcggca agcctgtgct
     3541 ggtctgccgc gaatcgaccg aacgcgggga gggcgtcgag gccggcacac ttcgcctggt
     3601 cgggaccgac ccggcaagga tccaggccga agccgacctg ctgctgaaga gcgaggctgc
     3661 ctaccaggcg atgagcgaag cggagaaccc gtacggcgac ggacgggcct cggagcggat
     3721 cgtcgcggcg cttaccaatc tctacgtcgg tggaaagatg ccggaacctt tccgctccac
     3781 ctacagccgc aacgccgtga tcaaggcgtc gggctacacc ctgccgggtg aggccagcgg
     3841 ccggctcgac cccgcgctgg tgccggagtc cgagcgtcat cacgaggagg cggtcgtgga
     3901 cccggtcggc aatctctggg ccgagttcgg aacgatcaca gagaagtggt tccgcacctg
     3961 atctttgccg agtggtaccc cttcctgggg ctggacccgt tcttcgaggt cctcttcctg
     4021 atcaccttcc tgatcgtggt cgcgatgttc ttctggacgg tcgtcctgtt cttccggggg
     4081 atcaagtccg atcgccacct ggcccggcag gaggaagtca acccggagct gttcgagtgg
     4141 atcttcctgg tcccggccct gaacgaggag gtgacgatcg ccgacagcgt cagccgtctg
     4201 gaagcgatcg aggtggcccg caagcggatc gtcgtgatca acgacggctc cgacgaccgc
     4261 accgcggaga tcctcgccga acgcaccgac ccggacctct acgtggtcga gcgcaaggcc
     4321 ccgcaggcga ggcagggcaa ggccgccgcc ctgaactacg cctttgacga ggtgatcaaa
     4381 cggttcaagc tcgatccgga gacgaccatc ttctgcatcg tcgatgcgga tggccgcatc
     4441 gccccggaca gcctgtcctt cgtcgcgaag catttcgtcg accctaaggt tgggggcgtt
     4501 cagaccctgg tccggatcta caaccgccac cggatcctga cctggttcca ggacatcgag
     4561 ttctcgatct acggccatct cttccaggcc gggcgcaaca agtggggcac cgccgggatg
     4621 ggcgggaacg gacagtacaa ccggatggcg gcgcttcagt cgatcgacga ccgtgacccc
     4681 ggcccggccg aggaggcgac cgtcgagaac gatccggagc cggaagtagc ggtcgagccg
     4741 gccagccg
//
LOCUS       DAOQFI010000041         4721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7553_length_4721_cov_3.257180, whole genome shotgun sequence.
ACCESSION   DAOQFI010000041 DAOQFI010000000
VERSION     DAOQFI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7553_length_4721_cov_3.257180"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLE93_01315"
     CDS             <1..658
                     /locus_tag="PLE93_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNH85715.1"
                     /translation="TERKVIGRSVDGRPIVSWHQGDPKGDFRVLVVGSVHGDERQGMR
                     VVARLRQNLAGRGGPKGIGVWSVKTANPDGTALGTRRNARRIDLNRNFPFRWDGSLNG
                     GYNSGPRPASEPETRAIMRLSRRVGFDLAVWFHQPWGRTLAPCDGQRRWAFLYARLSG
                     LGTGGSCDHGRYPGSAVSWQHQATGTTAFVVELGPGLQPRAVINRHARAVIRLAKRMK
                     "
     gene            complement(674..2692)
                     /locus_tag="PLE93_01320"
     CDS             complement(674..2692)
                     /locus_tag="PLE93_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH85716.1"
                     /translation="MAVFLGLLAVPATASALKFEAAPGTPLAPATSVIDPSPVMPPGF
                     SPTDIATGDFNKDGYGDIAVITDYISVQGPDGPEISPLSKNIYVYLGSADGSLESSAS
                     NPIGADSPIAGGRFMIRAIDVDGDGDLDLVTGSGGNPTIIEIYQNGFNGTHEAFATVP
                     GQTLSLDDSESAAGYRSVSLGDQDDDGDVDMALGLYDNRYVFIRNLSSLDGLEIPNPS
                     VEVSRDDPQDIDGITSTAIGNFGDGTGPDAFGDLVMVSSPGFRAENQPPDRLLYARGD
                     GASSFASPVELLRTGTDEYLGWVSKADLNGDQYDDIVFKVQTATGENIVRAALGSADG
                     PVIQAGEGSSISVYGAQQPAPGDFNGDGDQDVAIPQFVDDGFQVGLGDGSGRLALDFA
                     GPFALAAVDSADFFPQASATLDANGDGRLDFAAASGHSGDADQARGVAVMLNKPSSGI
                     SVSPTSIHFGDVPFDAELIAPVPVTIKSNGDLPLNLGQIGFSGPATGGFSLDRGNCPN
                     GNLASGSTCTLEVSMKQNQSGYFNGFLDITSDATGDPVRIEVIGQVGEAPPTARGLSL
                     KVKSAKKVKAGKKLVVSAIVRNSGKARSAHFTIRATAPKKMTGRIKFSPLRNLSLDGR
                     SYTTFFFKVPVKKQAKGVLRVKVSLVAKNRKIVRKTGEVRIVKKARRR"
     gene            2859..3200
                     /locus_tag="PLE93_01325"
     CDS             2859..3200
                     /locus_tag="PLE93_01325"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH85717.1"
                     /translation="MVEDQKEVDRVFHALSNQTRRGMVRQLSGGECSVGDLARPTRMS
                     FAAASKHVQVLEKAGMVNRRVEGRQHICSLRPDPLREASEWLSFYEKFWAGNLDRLAR
                     VVEADMKKEKK"
     gene            3197..3664
                     /locus_tag="PLE93_01330"
     CDS             3197..3664
                     /locus_tag="PLE93_01330"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH85718.1"
                     /translation="MTVSDQSVHLVKVVQAPPEMVYRAFIDPDELVRWMHPDAFTGVS
                     ASNDPRVGGRGELIHALDGEEVGGFNWQYLEVVPGRRLVMDWQFGPAGADPDGHHSRL
                     TIELREAGPGATEVSLTHDRLGDVPPGGPTGVSTGWTQALASLGRHFEAEGDE"
     gene            3667..4350
                     /locus_tag="PLE93_01335"
     CDS             3667..4350
                     /locus_tag="PLE93_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HNH85719.1"
                     /translation="MFEQMKTPPVVSAEEWDAARMELLVREKEVTRARDALAAERRRM
                     PWLKIEKDYEFEGPEGRVGLIDLFEGRRQLVLYRFFFDPDVSGHPDAGCPGCSFGADQ
                     VADLRHLNARDTTFAHASRGSQENIRRLKEKMGWEQIPWYTVIDDFDRDMGVGEWHGT
                     NAFIRDDDGTVYRTYFIDARGDEHMGSTWAHLDLTALGRQEEWEDSPEGYPQTPPYQW
                     WRRHDEYGS"
     gene            complement(4496..>4721)
                     /locus_tag="PLE93_01340"
     CDS             complement(4496..>4721)
                     /locus_tag="PLE93_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH85720.1"
                     /translation="GRTADWAFDLNAIECPARIVWGTEDKVLGWPIAAARYQEGLPDA
                     DWVILEGIGHCPMLDVPERTVELITEFAKD"
BASE COUNT          798 a         1526 c         1584 g          813 t
ORIGIN      
        1 gaccgagcgg aaggtgatcg gccgttcggt tgacgggcgg ccgatcgtga gctggcatca
       61 gggtgatccg aagggcgatt tccgggttct tgtggtcggt tcggtccacg gcgacgagcg
      121 gcaggggatg cgggtcgtgg ccaggctcag gcagaacctg gccgggcgcg gcgggccgaa
      181 gggcatcggg gtctggagcg tgaagaccgc caaccccgat ggcaccgcgc ttggcacccg
      241 tcgaaacgcc cgccggatcg acctcaaccg gaactttccc ttccgctggg acggcagcct
      301 gaacggcggg tataactcgg gcccgcgacc ggcctccgaa cccgagaccc gggcgatcat
      361 gcgactgagc cgccgggtcg gcttcgacct cgcggtctgg ttccaccagc cctggggccg
      421 gaccctcgcg ccctgtgacg gccagcgacg ctgggccttt ctctacgccc ggctctccgg
      481 cctggggacc gggggaagct gtgatcacgg ccggtatccg ggcagcgccg tctcctggca
      541 gcaccaggca accggcacca ccgccttcgt ggtcgaactc ggccccggcc ttcagccccg
      601 cgcggtcatc aaccgccacg ccagggccgt gatccgcctg gcgaagcgga tgaaatgaac
      661 gcaggcgaag gctctaccgc cgccgcgcct tcttcacgat cctgacctcg cctgtctttc
      721 gcacgatctt ccggttcttt gccaccaggc tcaccttgac ccggagcacg cccttcgcct
      781 gcttcttgac gggaaccttg aagaagaagg tcgtgtagct gcgaccgtcg agcgagagat
      841 tgcgaagcgg cgagaacttg atccttccgg tcatcttctt cggtgcggtg gccctgatcg
      901 tgaaatgcgc cgagcgggcc ttgccgctgt tgcggacgat cgcggacacg accagcttct
      961 ttccggcctt gaccttcttc gccgacttca ccttcaggga aagccccctg gcagtgggcg
     1021 gggcttcccc gacctggccg atcacctcga tccggaccgg atcgccggtc gcgtcgctgg
     1081 tgatgtcgag gaagccgttg aagtagccgg actggttctg cttcatcgag acctcgagcg
     1141 tgcaggtcga gccggacgcg aggttgccgt tcgggcagtt gccgcggtcg aggctgaaac
     1201 ctccggtcgc agggccgctg aagccgatct gaccgaggtt cagcgggaga tcaccgttgg
     1261 atttgatcgt gaccggaacc ggcgcgatca gctcggcgtc aaacgggaca tctccgaagt
     1321 ggatcgaagt cggtgagacg gagatcccgg aggagggctt gttcagcatg accgcgacgc
     1381 cgcgagcctg atccgcatca cccgaatggc cgctggccgc cgcgaagtcg agccggccgt
     1441 ccccgttggc gtccagggtg gcgcttgcct ggggaaagaa gtcagccgag tcgactgccg
     1501 ccagggcgaa cggtccggcg aagtcgagag ccagcctgcc gctgccgtcg ccgagaccaa
     1561 cctggaatcc gtcatcgacg aactggggga tcgctacgtc ctggtcaccg tcaccgttga
     1621 agtctcccgg ggccggctgc tgggcgccgt agacggaaat cgagctgcct tcgccggcct
     1681 ggatgaccgg tccgtcggcg ctgccgagcg cggccctgac gatgttttct ccggtggcgg
     1741 tctgcacctt gaagacgatg tcgtcgtact ggtcaccgtt cagatcggcc ttgctcaccc
     1801 agcccaggta ttcgtcggtt ccggttcgca gcagttcaac cggcgaggcg aagctcgatg
     1861 cgccatcgcc ccttgcgtaa aggagccggt ccgggggctg gttttcggcc cggaacccgg
     1921 ggctggagac catcaccagg tcgccgaaag cgtccgggcc ggtgccgtcg ccgaagttgc
     1981 cgatcgcggt ggaggtgatc ccgtcgatgt cctgcgggtc gtcgcgcgaa acctcgacgc
     2041 tcgggttcgg gatctcgagg ccgtccagac tcgacaggtt gcggatgaag acgtaacggt
     2101 tgtcgtaaag gccgagagcc atgtcgacgt caccgtcgtc gtcctgatcc ccaagcgaaa
     2161 ccgaccggta gccggcggcg gactcgctgt catccaggct gagcgtctgg ccgggaacgg
     2221 tggcaaacgc ctcgtgagtg ccgttgaagc cgttctggta gatctcgatg atggtcgggt
     2281 tgccgccgga cccggtgacc agatccaggt caccatcgcc atctacatcg atcgcccgga
     2341 tcatgaaacg cccgcccgcg atcggcgaat ccgcaccgat cggattgctt gccgatgact
     2401 cgagactgcc atccgcgctg ccgaggtaaa cgtagatgtt cttggagagg gggctgattt
     2461 ccggtccgtc gggcccctgg acggagatgt agtccgtgat cacggcgatg tcgccgtagc
     2521 cgtccttgtt gaagtccccg gtcgcgatgt cggtcgggga gaagccaggg ggcatgaccg
     2581 gggaagggtc gatgaccgag gtcgccggtg ccagcggcgt gccgggagcc gcctcgaact
     2641 tcagggccga agctgtggcc ggtacggcga gcaggccgag aaaaacggcc acgaacgtca
     2701 ctagcttgcg caagacaatc cccctcggat cggtcggttg atctcaccgt accgaacact
     2761 gtccgggtcc gttcgatgcg aaggggcggg cagtccgcca gaacttttgt tcaaccaacc
     2821 ggttgacaat cttccgtccg tgctctacgt tcaaccacat ggttgaagat cagaaggaag
     2881 tcgaccgggt gtttcacgcc ctctcgaacc agacccggag ggggatggtg aggcagcttt
     2941 ccggcggcga atgttcggtc ggcgacctcg cccggccgac ccggatgtcc tttgccgcgg
     3001 cctccaaaca cgttcaggtt ctggagaagg cggggatggt caatcgcagg gttgaaggcc
     3061 gtcagcacat ctgttcgctt aggcccgacc cgctcaggga ggccagcgag tggctgagct
     3121 tctacgagaa gttctgggca ggcaatctgg atcgcctcgc ccgggtagtg gaagcggata
     3181 tgaagaagga gaagaaatga ctgtctctga tcaatccgtt catctcgtca aggtcgttca
     3241 ggctcccccc gaaatggtct accgggcgtt catcgacccg gatgaactgg tccgctggat
     3301 gcaccccgat gccttcaccg gagtctcggc cagcaacgac ccccgggtcg gcggccgggg
     3361 cgagctgatc cacgccctgg acggggaaga ggtgggcggt ttcaactggc agtacctgga
     3421 ggtggttccc ggccggcgtc tggtgatgga ctggcagttc ggcccggccg gcgccgaccc
     3481 cgatggtcac cactcccgcc tgacgatcga gctgcgggaa gccgggccgg gagcgaccga
     3541 ggtttcgctg acccacgacc gccttggtga tgtgccgccg ggcggaccca caggagtcag
     3601 cacaggctgg acccaggctc tggcgagtct cgggcggcat ttcgaagcag aaggagatga
     3661 gtagtcatgt tcgagcagat gaagacgcca ccggtcgtct cggccgagga atgggatgcg
     3721 gcccggatgg agttgctggt ccgcgagaag gaagtgacca gggccaggga cgcgctggcc
     3781 gccgaacgcc ggcggatgcc ctggctgaag atcgagaagg actacgagtt cgaaggcccc
     3841 gagggcaggg tcgggctgat cgaccttttt gaaggccgcc gccagcttgt gctctaccgc
     3901 ttcttcttcg acccggatgt aagcggccac cccgatgccg gttgccctgg ctgctcgttc
     3961 ggggccgatc aggtcgccga cctccggcat ctcaatgccc gggacacgac cttcgcccat
     4021 gcttcccggg gcagccagga gaacatccgg cggctgaagg agaagatggg ctgggagcag
     4081 atcccctggt acacggtgat cgacgacttc gaccgcgaca tgggtgtcgg cgagtggcac
     4141 ggaaccaatg ccttcatccg tgatgacgac ggcacggtct atcgcaccta cttcatcgac
     4201 gcgcgcggcg acgagcacat gggcagcacc tgggcccacc tcgacctgac cgcgctcggc
     4261 cgccaggaag agtgggagga ttcgcccgag ggttatccgc agaccccgcc gtaccagtgg
     4321 tggcgccgcc acgacgagta cggctcatga tcctcgccca catcggcccg ctcccggtcg
     4381 aagaactcct cgccctcgcc ccggccctca ctggcgccac cctcgccctc cgcgcccggc
     4441 tccgaaaccg ccgcggcttc tgagccgcta aatctgcccg gccaggcggg ccgggtcagt
     4501 ccttcgcgaa ttcggtgatc agctcgaccg tccgctcggg cacgtcgagc atcgggcagt
     4561 ggccgatccc ttcgaggatc acccagtcgg catccggaag tccctcctgg tagcgggccg
     4621 cggcgatcgg ccagcccagc accttgtcct cggtgcccca gacgatcctg gccgggcact
     4681 cgatggcatt caggtcaaac gcccaatccg cggtgcggcc c
//
LOCUS       DAOQFI010000042         4690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7634_length_4690_cov_2.730744, whole genome shotgun sequence.
ACCESSION   DAOQFI010000042 DAOQFI010000000
VERSION     DAOQFI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7634_length_4690_cov_2.730744"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLE93_01345"
     CDS             complement(<1..698)
                     /locus_tag="PLE93_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNH85721.1"
                     /translation="MIETFTLRDVGPKDGLEQAEMVAGRITDFIGKAEQSLVLALYDI
                     RLPDPVGETVAQAFRDAAARGVKVRLAYNDVPEDPDAGNFLPAVNPPPETNPEILARL
                     PIETKPIKGIPDLMHHKYMVRDEKAVWTGSSNWSVYSWTRQENVLGIIDHGEVAREYL
                     TDFEELWQKHKVERSGFGDPNPIRVGEATVRVWFTPGHGKALAQRISSKLGSARRRIR
                     IASPVLTSGPILSTL"
     gene            complement(695..1126)
                     /locus_tag="PLE93_01350"
     CDS             complement(695..1126)
                     /locus_tag="PLE93_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNH85722.1"
                     /translation="MSAATAEPKTDRKIRVVVAKPGLDGHDRGAKIIARALRDSGMEV
                     IYTGLHQTPEQIVETVIQEDADAIGVSILSGAHMTLVPKILELLEAEGIDDVLITVGG
                     TIPNKDIDALKEMGVSGVFTPGAPTDDIVNFIRTGVSRREL"
     gene            complement(1150..2787)
                     /locus_tag="PLE93_01355"
     CDS             complement(1150..2787)
                     /locus_tag="PLE93_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH85723.1"
                     /translation="MEPTSTSSSSDAARKAAEAADNEFTTMSGKPIKARYGPEDLSGD
                     ADVKIGEPGQYPFTRGPYESMYRGRNWTMRQFAGFGTVEETNERFHYLLDHGQTGLST
                     AFDMPTLMGYDSDHARSLGEVGREGVAVDTIDDMEILFQGIPLGEVSTSMTINAPAAI
                     LLAFYVLVGEQQGVASEKLSGTIQTDILKEYIAQKEWCFPVEPAMRLVTDMVEYCTDH
                     MPRWHPISISGYHIREAGATAAQELAFTLKDGFTYVERAIERGIDVDAFAPRLSFFFN
                     AHIDFFEEIAKYRAARRIWARELKETYGAKKPESMRLRFHTQTAGVSLTAQQPLNNIV
                     RTAIEALAGVLGGTQSLHTNSFDEALALPTEEAVRVALRTQQIIADETGVTNTPDPLG
                     GSYFVESLIDELEADAYDYFRRIDELGGMVEAVRQNFPQREIGEAAFDFQRRVDDGEF
                     TIVGVNQYVTEEEEVPLLHIDPALETKQVERLAATRAKRDSAEVESALTALKDAAAGE
                     ANLMYPILDAARVHTTEGEMIEAMQQVFGTYTETPVF"
     gene            complement(2873..3643)
                     /locus_tag="PLE93_01360"
     CDS             complement(2873..3643)
                     /locus_tag="PLE93_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNH85724.1"
                     /translation="MVQAAFFDLDKTLMAGSSGMQFAKAAQERGLISRAQILRWGRDH
                     IRYRIKGATDDETGEILKVARETLSGVSYNEISRMWPEVLAGILPRIYPEVLAEVHRH
                     QDEGRLTYIVSAAGSDMVESLAQVLGMDGGIGTRYVVEDGIYTGALDGPFVYGRGKVK
                     AIRWLSELREQTGEEPFDLEGSWAYSDSASDLPMMELVGNAVAVNPDAPLLEKARREG
                     WTVMRFDRLGRNLAIGGTVAGALLVGGIGGWLSKRRAG"
     gene            3661..>4690
                     /locus_tag="PLE93_01365"
     CDS             3661..>4690
                     /locus_tag="PLE93_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85725.1"
                     /translation="MAENEGERPREMRKTLIVMLLWLVAGITPALASPPPGSDWVQTN
                     RVQFSGTDGFFRSQGVASDGQSLFFSWNLGLSRTAINDTSNVLADNTTEAIPDDLAES
                     NHNHIGDIDVAWGWIYAPIEDGPAYAEPWVVIYDADTLEPTGQRFLLPASLQRDGVPW
                     IAVDQVRGVAYSMEWKDTGKLFVYSLSDFSLLRTVTLSEPVPRIQGAKIYRGNLYASR
                     DNGSEKSIVAIDPETGTVTHLFDRNLGEDYEAEGIAFVRRSSGTMMVATGIREGSQGY
                     TEMRIYRIGGDTTAPALSGVSFKAKRVRPGSKVTLLATSSEPVTALVRWLRCIGPAAR
                     PCSRTRRSS"
BASE COUNT          769 a         1519 c         1560 g          842 t
ORIGIN      
        1 agggtgctga ggatcgggcc ggaagtcagg accggcgagg cgatccggat ccggcggcgg
       61 gccgaaccga gcttcgacga gatccgttgg gcaagcgcct tgccgtgacc gggggtgaac
      121 cagacccgga ccgtggcctc ccccaccctg atcggattgg gatcaccaaa gccggatcgc
      181 tcgaccttgt gcttttgcca gagttcctcg aagtcggtca ggtattcgcg ggcgacctcg
      241 ccgtgatcga tgatccccaa cacgttctcc tgccgggtcc aggagtagac cgaccagttt
      301 gaggagccgg tccagaccgc cttctcgtcc cgcaccatgt acttgtggtg catcaggtcg
      361 gggatgccct tgatcggctt cgtttcgatc ggcagtctgg ccaggatctc cggattggtc
      421 tccggcggcg ggttcacggc cggcaggaaa ttgccggcat cgggatcttc cggcacgtcg
      481 ttgtaggcga gtcgcacctt gactccccgg gccgcggcat cccggaaggc ctgtgccacg
      541 gtctccccga ccgggtccgg cagccggatg tcataaaggg cgaggaccag gctctgctcg
      601 gccttgccga tgaagtcggt gatcctcccg gccaccatct ccgcctgctc cagcccgtcc
      661 ttcggaccaa cgtcacggag ggtgaaggtt tcgatcacag ctcgcggcgg ctgacgccgg
      721 tgcggatgaa gttgacgatg tcgtcggtcg gggcgccggg agtgaagact cccgagacgc
      781 ccatctcctt gagcgcgtcg atgtccttgt tcgggatcgt gccgccgacc gtgatcagga
      841 cgtcgtcgat gccttcggct tcgagcagtt cgaggatctt cgggaccagg gtcatgtggg
      901 caccggagag gatcgagaca ccgatcgcgt cggcgtcctc ctgaatcacc gtttccacga
      961 tctgctccgg ggtctggtga aggccggtgt agatgacctc catgccggag tcgcgcaggg
     1021 cgcgggcgat gatcttggct ccccggtcgt ggccgtcgag gccgggcttg gccacgacca
     1081 cgcggatctt gcggtcggtc ttcggttcgg cagtggcggc gctcatcagc tgaaaacctg
     1141 ctttcggatc tagaagaccg gagtctccgt gtaggtgccg aatacctgct gcatcgcctc
     1201 gatcatctcg ccttcggtcg tgtggacccg ggcggcatcg aggatcgggt acatcaggtt
     1261 ggcctcgcct gccgcggcat ccttgagggc cgtcagggcg gactcgactt cagccgagtc
     1321 gcgttttgcc ctggtcgcgg cgagacgctc gacctgcttg gtctcgaggg ccggatcgat
     1381 gtgaaggagg ggaacctcct cttcctcggt cacgtactga ttgaccccga cgatcgtgaa
     1441 ctcgccgtcg tccacccggc gctggaagtc gaaggccgct tcaccgatct cgcgctgggg
     1501 gaagttctgg cggaccgctt cgaccatgcc gcccagttcg tcgatccggc ggaagtagtc
     1561 gtaggcgtcg gcttccagct cgtcgatcag cgactcgacg aagtacgaac cgccgagcgg
     1621 atcgggcgtg ttggtgactc cggtttcgtc ggcgatgatc tgctgggtcc gcagcgcgac
     1681 ccggaccgct tcctcggtcg gcagggcgag ggcctcgtcg aaggagttgg tgtgaagcga
     1741 ctgggtgccg ccgagcaccc cggccagggc ctcgatcgcg gtccggacga tgttgttcag
     1801 cggctgctgt gcggtcagcg agaccccggc ggtctgcgtg tggaagcgaa gccgcatcga
     1861 ctcgggcttc ttcgctccgt aggtctcctt gagctcacgg gcccagatcc ggcgggcggc
     1921 gcggtacttg gcgatttcct cgaagaagtc gatgtgggcg ttgaagaaga acgaaagtct
     1981 gggcgcgaag gcgtcgacgt cgatgccgcg ctcgatcgcc cgttcgacgt aggtgaaccc
     2041 gtccttgagg gtgaaggcga gttcctgcgc ggcggtagcc ccggcctcgc ggatgtggta
     2101 cccggagatc gagatcggat gccagcgcgg catgtggtcg gtgcagtact cgaccatgtc
     2161 ggtgaccagg cgcatcgccg gctcgaccgg aaagcaccac tccttctggg cgatgtactc
     2221 cttgaggatg tcggtctgga tcgtgcccga gagcttctcc gaggcgacgc cctgctgctc
     2281 gccgaccagc acgtagaagg cgagcaggat cgccgccggg gcgttgatcg tcatcgaggt
     2341 cgagacctcg ccgagcggga tgccctggaa gaggatctcc atgtcatcga tcgtgtcgac
     2401 ggcgacgcct tcgcggccga cctcgcccag cgatcgcgcg tggtcggagt cgtagcccat
     2461 cagggtcggc atgtcgaagg cggtcgaaag cccggtctgg ccgtgatcga gcaggtaatg
     2521 gaagcgctcg ttggtttcct cgacggtgcc aaagccggcg aactggcgca tcgtccagtt
     2581 gcgaccccgg tacatcgatt cgtaaggacc ccgggtgaag gggtactggc ccggctcgcc
     2641 gatcttgacg tcggcgtcgc cgctcagatc ctcgggaccg taccgggcct tgatcggctt
     2701 gcccgacatg gtcgtgaact cgttgtcagc ggcctcggcc gccttgcggg cagcgtccga
     2761 agaactggat gtggaagtcg gctccatagg cgaggtccat gttaaggccg ttcggaccac
     2821 cggcagcgga gcccctcgat ccactagtgg atatccacga gaggcgggtt cctcagcccg
     2881 cccgtctttt cgaaagccag ccgccgatcc cgccgaccag gagggcgccg gcgacggtgc
     2941 cgcctatcgc gaggttgcgg ccgagtcgat cgaagcgcat caccgtccag ccttcccggc
     3001 gggccttctc gagcagcggg gcatccgggt tgaccgcgac cgcgttgccg accagctcca
     3061 tcatcggcag gtcggaggcc gagtcggagt aggcccagga gccttcgagg tcgaacggtt
     3121 cctcgccggt ctgttcgcgg agctccgaaa gccagcggat cgccttgacc ttgccccggc
     3181 cgtagacgaa gggaccgtcg agcgccccgg tgtagatgcc gtcctcgacc acgtatcggg
     3241 tgccgatgcc gccgtccatg cccagcacct gggccagcga ctcgaccatg tccgatcccg
     3301 ccgcactgac gatgtaggtg agtctgcctt cgtcctgatg ccggtgaacc tcggccagta
     3361 cctcggggta gatccggggc aggatgccgg ccagcacttc cggccacatc cgggaaatct
     3421 cgttgtagga aacaccggaa agcgtttccc gggcgacctt gaggatctcg ccggtttcgt
     3481 cgtcggtggc gcccttgatc cggtaacgga tgtgatcccg gccccagcgc aggatctggg
     3541 ccctcgaaat cagccctctc tcctgggccg ccttggcgaa ctgcatgccg gaggagcccg
     3601 ccatcagggt cttgtcgagg tcgaaaaatg ctgcctggac catgaacgga tacgttagcg
     3661 atggctgaaa acgagggaga aaggccgcgg gagatgagaa agacgctgat cgtgatgctg
     3721 ttgtggctcg tggccggcat aacgcccgcc ctggccagcc cccccccggg ttccgactgg
     3781 gttcagacca accgggtcca gttcagcggc accgacggct tcttccgctc gcagggcgtg
     3841 gccagcgacg ggcagtcgct cttcttctcc tggaacctcg gcctcagccg caccgcgatc
     3901 aacgacacct cgaacgtgct ggccgacaac acgaccgagg cgatccccga cgatctggcc
     3961 gaaagcaacc acaaccacat cggcgacatc gacgtggcct ggggctggat ctacgccccg
     4021 atcgaggatg gccccgccta cgccgaaccc tgggtggtga tctacgacgc cgacaccctt
     4081 gaaccgaccg gccagcgctt cctgttgccc gcctcgctcc agcgtgacgg cgtcccctgg
     4141 atcgccgtgg accaggtccg tggcgtcgcc tactcgatgg agtggaaaga caccggcaag
     4201 ctcttcgtct acagcctgag tgacttcagc ctgctccgca ccgtgaccct gagcgagccg
     4261 gtcccccgca tccagggagc gaagatctac cgcggcaacc tctacgcatc gcgtgacaac
     4321 ggcagcgaga agtcgattgt cgcgatcgac ccggagaccg ggaccgtcac gcacctcttc
     4381 gaccgcaacc ttggcgaaga ctacgaggcc gagggcatcg ccttcgtccg ccggtcctcc
     4441 ggcacgatga tggtggccac cggcatccgt gaaggctcac agggctacac cgagatgcgg
     4501 atctaccgga tcggcggcga cacgaccgcc ccggcactga gcggggtcag cttcaaggca
     4561 aaaagggtca gaccgggctc gaaagtgacc ctgctcgcca cctcttccga gccggtcacc
     4621 gcgctggtca ggtggctcag gtgcatcggt ccggcggcca ggccctgctc gaggacccgg
     4681 cggtcctcaa
//
LOCUS       DAOQFI010000043         4672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7687_length_4672_cov_3.556855, whole genome shotgun sequence.
ACCESSION   DAOQFI010000043 DAOQFI010000000
VERSION     DAOQFI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7687_length_4672_cov_3.556855"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PLE93_01370"
     CDS             <1..1466
                     /locus_tag="PLE93_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85726.1"
                     /translation="GDPTPGWPVEVKLPDSIKSTIPANSYIHDPKLMMAPAVGDVLGT
                     GTDQVFLPGFDCDDAANKTFAYGIQSDGNNNPGGPYLPGWPIKMGSIGGCYSQSIDFV
                     QEGANAASIGDFDNSGQLRIAITPVAGSPVIVNGDGSLYKGMTPGCTSAPCSGYDPYY
                     GSDSITVGVTSQSAIGDLTGDGMPEFMQPTAGGATLSGALNDVGQAALAHVFDQAWNP
                     TTGAVLDNFPTDQDGFPFYASPVVANLTDDGNRSMITANDSYWIHARGPDGQEAPGFP
                     KWTGQWTSFGGAVGDPKFDGQQYLAYGTREGQVFLWKVGGKPSMNDQWWHYRHDEHNS
                     GRYGNDTRPPAGVKFVVKRSKKSAQVTWKASGDDGVNGDKVAVLQVYKSNRKISLSQL
                     GSKVKSPAIAKPNGQQKVNLKLAPNKSLYVAVRQQDADGNWSSLSRFKIKPFKSYNLK
                     KQKKICRKKFRGSKRKQCIRKAEKKAKAVAKQNTQRS"
     gene            complement(1692..2468)
                     /locus_tag="PLE93_01375"
     CDS             complement(1692..2468)
                     /locus_tag="PLE93_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNH85727.1"
                     /translation="MSDATRSSQGGSAVLEIQGVTKAFGERQVLGGIDLTLREHEAIA
                     LIGASGSGKSTLLRCIDLLVEIDDGDIFLDGEVITDPSVDPVSVRRKLGFVFQAFNLF
                     PHMKVIDNVTLGSVRAQGSGKKEAREEGRALLERFGLGGREEDMPDELSGGQQQRVAL
                     CRALAGNPRALLLDEVTSALDPELVGDVLDAVRDLKQEGMTMILATHEMSFAREVADE
                     VAFLHEGRIIEQGSPGQILSEPNRPETRKFLRRLLAAGRL"
     gene            complement(2465..3136)
                     /locus_tag="PLE93_01380"
     CDS             complement(2465..3136)
                     /locus_tag="PLE93_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNH85728.1"
                     /translation="MFFSAESFRDSFPDILKAFWIDIKLFMVVEAIVLVLGLLIAVVR
                     TSKASALFPIRMLAAVYCDFFRGIPVILLVYLVGFGIPALGLAGVPTDPLILAGIALS
                     LAYSSYVAEVYRAGIASIHPSQNDAGLATGLTRIQTLRFVIVPQAVRRVRPPLLNDFI
                     SLQKDVALVSVLGIAEAFRTAQIITLSTFNYTPLLAAALLYLAVTFPMARILDHITAR
                     EQINS"
     gene            complement(3323..4204)
                     /locus_tag="PLE93_01385"
     CDS             complement(3323..4204)
                     /locus_tag="PLE93_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH85729.1"
                     /translation="MSRKRLVLFLFAIFALAVFVGCGSSDDDSSSENSGTDSGSTVAA
                     SDCTPANLSTFKDGVLTIGTDSPAYPPYFEDDDPTNGKGFESALGYAIAGELGFNDSQ
                     VEWATVPFNASYAPGPKDFDFDLNQISITPAREKAVTFSEPYYTANQAVLVKKGSDLD
                     GITSLDQLKDASIGVQIGTTSYDAVVEQIDPSSDPKTFDNSNDVVTAFKQDQVDAIVV
                     DLPTAIYLRDAELSDTVVAGQFEQEGGDQWGALMAKDSDLKGCVDQAINTLKEDGTLE
                     EITNQWMADSAKAPLLN"
     gene            4277..>4672
                     /locus_tag="PLE93_01390"
     CDS             4277..>4672
                     /locus_tag="PLE93_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNH85730.1"
                     /translation="MSTEPKSQAVRDEEEGRPCVEMSEGVARYLMALFDLEQEGGPPT
                     QARLARELDVSQPSALQMVRRIRAMGLINEGDLGLTPAGTSSALVLTSRRTAATDLTS
                     EVLGLDPAEARVEAGHLASSATPAFGRKLV"
BASE COUNT          879 a         1473 c         1551 g          769 t
ORIGIN      
        1 atggcgatcc gaccccgggc tggccggtcg aggtcaagct gccggattcg atcaagtcga
       61 cgatcccggc gaacagctac atccacgatc cgaagctgat gatggctccg gccgtcggtg
      121 acgtgctcgg caccggcacc gaccaggtct tcctgcccgg attcgactgc gacgatgcag
      181 cgaacaagac cttcgcctac ggcatccagt ccgacggcaa caacaacccg ggcggacctt
      241 acctgccggg ctggccgatc aagatgggtt cgatcggagg ctgctactca cagtcgatcg
      301 acttcgtcca ggaaggggcc aacgcggcct cgatcggtga tttcgacaac tccgggcagc
      361 tccggatcgc gatcactccg gtggccggct caccggtcat cgtcaacggt gacgggtcgc
      421 tctacaaggg tatgaccccc ggctgcacct cggccccctg ctcgggctac gacccttact
      481 atggctcgga ttcaatcacc gtcggcgtga ccagccagtc ggcgattggc gatctgaccg
      541 gggacggcat gcccgagttc atgcagccga cggctggtgg tgccacgctc agcggggccc
      601 tcaacgatgt cgggcaggcg gctctcgccc atgtcttcga ccaggcctgg aatccgacca
      661 ccggagcggt gctggacaac tttcccaccg accaggacgg cttcccgttc tacgcctcac
      721 cggtggttgc caacctgacc gatgatggca accgctcgat gatcactgcc aatgactcct
      781 actggatcca cgcccggggc ccggacggcc aggaagctcc cggtttcccg aagtggaccg
      841 gccagtggac ctcgttcggc ggtgcggtcg gtgacccgaa gttcgacggc cagcagtacc
      901 tggcctacgg cacgcgggaa ggccaggtct tcctctggaa ggtcggcggc aagccctcga
      961 tgaacgacca gtggtggcac taccgacacg acgagcacaa ctcgggccgt tacggcaatg
     1021 acacccggcc cccggccggc gtcaagttcg tggtcaaacg ctcgaagaag tcggcccagg
     1081 tcacctggaa ggcctccggt gacgatgggg taaatggcga caaggtcgcc gtgctccagg
     1141 tctacaagtc gaaccggaag atcagcctgt cgcaactcgg cagcaaggtc aagtcgccgg
     1201 ccatcgccaa gccgaacggc cagcagaagg tcaacctcaa gctggctccg aacaagagcc
     1261 tctacgtcgc cgtccgccag caggacgcgg acgggaactg gtcatcgctc agccgcttca
     1321 agatcaagcc cttcaagagc tacaacctga agaagcagaa gaagatctgc cgcaagaagt
     1381 tccgtggcag caagcgcaag caatgcatcc gcaaggccga gaagaaggcc aaagcggtag
     1441 cgaagcagaa cacccagcgg tcctagcggg aggtgccgca ggttctggcc cgatatgggt
     1501 gcaaaacctg cggcaccttt tgattcccgg cggatcggtt catgccccgg aaccggcact
     1561 cggtggtgac gccgtggggc cgggtggccc tcccaaagat ggcgccaccg tcgcgcttgt
     1621 cctcacctgc tcgctgaccg gagcagtaat cgagaagcca ctttgggagg gccacccggc
     1681 cccacggcca actagaggcg gccagcggcg aggaggcggc ggaggaactt gcgggtttcg
     1741 gggcgattcg gttccgagag gatctgcccg gggcttccct gttcgatgat ccggccttcg
     1801 tggaggaagg cgacctcgtc ggccacctcg cgggcgaagc tcatttcgtg ggtggcaagg
     1861 atcatcgtca tcccttcctg cttcagatcc cggacggcat cgagcacgtc gccgaccagt
     1921 tcggggtcga gggcgctggt cacctcgtcc aggaggaggg cccggggatt gccggccagg
     1981 gcccggcaga gggcgacccg ctgctgctgg ccaccggaaa gctcgtccgg catgtcctct
     2041 tcgcggccgc cgagcccgaa acgctccagc agggcccttc cctcctcgcg ggcttccttc
     2101 ttccccgatc cctgggcccg gaccgagcca agcgtgacgt tgtcgatcac cttcatgtgc
     2161 gggaagaggt tgaaggcctg gaagacgaag ccgagcttgc gccggaccga gaccggatcg
     2221 accgaggggt cggtgatcac ctcgccgtcg aggaagatgt cgccgtcatc gatctcgacc
     2281 agcaggtcga tgcagcgcag cagggttgac ttgccggagc cggaagcgcc gatcagggcg
     2341 atcgcttcat gctcccgcag ggtgaggtcg atgccgccga gtacctgacg ctcgccgaag
     2401 gccttggtca caccctggat ttccaggacc gctgaacctc cctggctgga acgcgtggcg
     2461 tcgctcacga gttgatttgc tcccgggcgg tgatgtgatc aaggatccgc gccatcggga
     2521 aggtaaccgc caggtagagc agggcggcgg ccagcagcgg ggtgtaattg aaggtcgaaa
     2581 gcgtgatgat ctgggcggtc ctgaacgcct cggcaattcc gagcacggaa accagtgcaa
     2641 cgtccttctg gagcgagatg aagtcgttga ggagcggcgg ccgcacccga cgcaccgcct
     2701 ggggcacgat cacgaaacgc agcgtctgga tccgggtcag cccggtggcc agaccggcgt
     2761 cattctggct ggggtggatc gaggcgatgc cggcccggta gacctccgcc acatatgagc
     2821 tgtaggcgag cgaaagggcg atgccggcga ggatcagggg atcggtaggc accccggcca
     2881 gcccgagggc ggggatgccg aagccgacca ggtaaacgag caggatgacc ggtatcccgc
     2941 ggaagaagtc acagtagacc gccgccagca tccggatcgg gaagagggcc gatgccttgc
     3001 tggtcctgac caccgcaatc agcaatccga ggaccaggac gatggcctcg acgaccatga
     3061 acagcttgat gtcgatccag aaggctttga ggatgtccgg aaacgagtcc ctgaaggact
     3121 cggccgagaa gaacatctcc cgcacgtccg gccagccgct gctggtcagg atgacggcgg
     3181 caaggccacc gagaaccagg accgatgaaa gaacggcgat cgcctggccg cgccgcgccc
     3241 tggcctttcg ggccttgtcg cgggcggccc ggcgatcgcc ggtaccgctt tgaactggag
     3301 cttccgtggt cagacccggc tcttagttga gcagcggcgc cttggccgaa tcggccatcc
     3361 actggttggt gatctcttcc agcgtgccgt cttccttcag cgtgttgatc gcctggtcca
     3421 cgcaaccctt gaggtcggag tccttggcca tcagcgcgcc ccactggtcg ccaccctcct
     3481 gctcgaactg accggcaacg acggtgtcgg agagctcggc gtcacggagg tagatcgcgg
     3541 tcggaaggtc gaccacgatc gcgtcgacct gatcctgctt gaaggccgta acgacgtcat
     3601 tcgagttgtc gaaggtcttc gggtcggatg agggatcgat ctgctcgacc acggcgtcgt
     3661 agctggtggt accgatctgg accccgatcg aagcgtcctt gagctggtcg aggctggtga
     3721 tcccgtcgag atcagagccc ttcttgacca gcaccgcctg gttggcggtg tagtacggct
     3781 ccgagaaggt gaccgccttc tcgcgggcgg gagtgatcga gatctggttc aggtcgaagt
     3841 caaagtcctt cggtccgggc gcgtaggaag cgttgaacgg cacggtcgcc cattcgacct
     3901 gggagtcgtt gaatccgagc tcgccggcga tcgcgtaacc gagggcgctc tcgaagccct
     3961 tgccgttggt cgggtcgtcg tcctcgaagt acggcgggta ggccggggag tcggtgccga
     4021 tggtcagcac accgtccttg aaggtgctga ggttcgccgg agtgcagtcc gaagcggcga
     4081 cggtgctgcc actgtcggtg ccggaatttt cgctggacga gtcatcgtca ctggagccgc
     4141 agccgacgaa aacggccagg gcgaagatgg caaacaggaa aaggacaaga cgtttacgac
     4201 tcacgagtat tcctccgcag gttgagcgac tgacgtgggg agcctagagc atgttgaggg
     4261 tagaatcgct cgcggtttga gcacagagcc gaaaagtcag gccgtacgcg acgaggagga
     4321 aggccgtccc tgcgttgaga tgagcgaagg cgtggcccgt tacctgatgg cgctgttcga
     4381 cctcgagcag gaaggcggcc ccccgaccca ggccaggctg gcccgggaac ttgacgtttc
     4441 ccagccctct gcgcttcaga tggtccgccg gatccgggcg atgggcctga tcaacgaggg
     4501 ggacctcgga ctcacgccgg ccggcaccag ctccgctctg gtgctcactt ccaggcggac
     4561 cgccgcaacc gacctgacca gcgaggtgct gggactcgac ccggccgaag cgcgggttga
     4621 agccggacat ctcgcctcca gcgccacccc ggccttcggc cgcaagctgg tc
//
LOCUS       DAOQFI010000044         4659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7717_length_4659_cov_3.345786, whole genome shotgun sequence.
ACCESSION   DAOQFI010000044 DAOQFI010000000
VERSION     DAOQFI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_4659_cov_3.345786"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLE93_01395"
     CDS             <1..507
                     /locus_tag="PLE93_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85731.1"
                     /translation="FLREYTDPEIDPAEPPHAFEHVLLDRLPPEAVQLTAEFAQPVTG
                     CGAMMVEIRHMGGALGRPAPGGGASDRIDGEYLFFVVGFPDVAETMNHAVDVLSDFGR
                     GRTYFNFMPRRTGRETAYGQEAIARLADLYEAVDPEGLIDHPHPISRTASVADETATT
                     QVLAILHG"
     gene            615..3989
                     /gene="mfd"
                     /locus_tag="PLE93_01400"
     CDS             615..3989
                     /gene="mfd"
                     /locus_tag="PLE93_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNH85732.1"
                     /translation="MTLRPTIDLLLEDEQISRLSAEVREAVAGSAGTTVRAEVSATLR
                     PALIAAMLEDDFGLEDRPALIVTPDDRSAREMAAALGAFLGDRMVRSYPSRGTGYASQ
                     VTPPPHLTGLRIDALDSFGREEAHPPVVVASAIALAESVPDASLRPAGFRLTVGEEHD
                     PSQVAADLVAAGYERVDQVQERGQFAVRGGILDVFSATEDRATRVDFFGDEIESMRWF
                     STFTQRSLGQAESIELSPAAELDAEHREMAELIIAEARENETDLDLTEALPLDHFRAP
                     LDLIPDSTAVILTADEEIEPALKDHWEDVTTAMHADDARHLYEDVAGPLNQRASLILV
                     RPGEGEGALRAMSAETPARNLKEAEAELRKLVEAGYRTIVAFDSRGEAERARYGFDRL
                     DTKIVDQGPVSIEPGVSLVESRLAEGFVSPRTKVAVLPFRRLLNRRRRADERAPEAVR
                     GRLTFSELRVGDFVVHEDHGVARFAGFETREVGGITRDYLYLEYKGEDRVYVPTDQFA
                     KLSRYSGGAGTPTLSALGGRKWLNMKARARGAADEIAGELVNLYAERKTRKGHAFSPD
                     SEWQMELEANFPWRETADQIEAIEAVKADMESEQPMDRLVCGDVGYGKTEVAIRAAVK
                     AASDGKQVMILAPTTILAQQHLGTFRERLADLPFRVDGVSRLRKPAEVKQVVAEWGEG
                     KVDVLIGTHRLLSRDVRAKDLGLIVVDEEQRFGVKQKELLRQMKLKVDVLSMSATPIP
                     RTLQMSMSGLRDISVIETPPEGRRPVRTYVGPWDEQLVERAIKREISRKGQVFFLHNR
                     VESLDEAAESLRALVPGVRIQVAHGQMEDQELEAAMLNFLRGDADCLVATTIIESGID
                     IPAANTLIVDRADHLGLAQAYQIRGRVGRSRERAFAYLLYPSEESLTAEASTRLATLA
                     DHTELGSGFRIAMRDLDIRGAGNLLGDEQSGHVAAVGFELYCQLIDEAVADLAGREES
                     AGEPEPVRFDIGVNAYLPASYVPFEAAKIDIHRRIAAARRPGDLRQITDELIDRFGPL
                     PEDVENLITLQQARIDLGSAGVEQVQFRGGKLTVSGIELDSEAAAAIRESVPGALYEW
                     RSHELSVRIDNDPAKRLATVSELGRALADVGMLRQS"
     gene            4033..>4659
                     /locus_tag="PLE93_01405"
     CDS             4033..>4659
                     /locus_tag="PLE93_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH85733.1"
                     /translation="MSGARKFGLILFGAILVVLFAGIAIAQGVSKPGVESGDIITVQD
                     VPDGKGSITEEDYNRSFEQTWKRGGLQAAPKEGDAQYDQVKEAAINDLLDQAWLAGVR
                     RLLFLGSSCIYPKHAAQPMSEEALLTGTLEPTNEAYAIAKIAGIKLCESYNRQYGTDY
                     RSVMPTNLYGPGDHYDLQNSHVIPAMIRKLHLAKLATGHDWAAIEQDEA"
BASE COUNT          810 a         1632 c         1518 g          699 t
ORIGIN      
        1 ttcctgcggg agtacaccga cccggagatc gatccggcag agccgcccca tgccttcgag
       61 cacgttctgc tcgatcggct tcccccggag gcggtccagc tgaccgccga gttcgcccag
      121 ccggtgaccg gctgcggggc gatgatggtg gagatccgcc acatgggggg agcgctgggt
      181 cgaccggccc ccggcggcgg cgccagcgac cggattgacg gcgagtacct gttcttcgtg
      241 gtcggtttcc ccgatgtggc ggagaccatg aaccacgccg tcgatgtcct ctcggacttc
      301 ggccgcggtc ggacctactt caacttcatg ccccggagga ccggtcgcga gaccgcctac
      361 gggcaggaag cgatcgcccg gctcgccgac ctctacgaag ccgtcgatcc ggagggtctg
      421 atcgaccatc cccacccgat ttcccgcacc gccagcgtcg ccgacgaaac cgccacgacc
      481 caggtcctgg caatcctcca cggctgacag tcgcccggcg gggcaacctc aaacgcagaa
      541 atacaggcgc ccggcggtct ctccctgacc accgggcgct tttcgctttc aggttcgtgc
      601 tttgatcggt ggccgtgacc ctgcgcccga caatcgacct gctgctcgag gacgagcaga
      661 tctcccgcct ctcggccgag gtccgcgagg ccgtcgccgg ttccgccgga acaacggtcc
      721 gggcggaagt ctccgccacc ctgcggccgg ccctgattgc cgccatgctc gaagatgact
      781 tcggcctgga agaccgcccg gccctgatcg tcacgcccga cgaccggtcg gcccgggaga
      841 tggccgccgc gctgggggca ttcctcggtg accgcatggt tcgctcctac ccttcccgcg
      901 gcaccggcta tgcctcgcag gtaaccccgc cgccgcatct gaccggcctc aggatcgacg
      961 ccctcgattc cttcggccgc gaggaggctc accccccggt cgtggtcgcc tccgcgatcg
     1021 cgctcgccga atcggtgccc gacgcttcgc ttcggccggc cggcttccgg ctcacggtcg
     1081 gcgaggaaca tgatccctcg caagtcgctg ccgacctggt cgcggccggc tacgaacggg
     1141 tggaccaggt ccaggaacgc ggccagttcg cggtccgggg cggcatcctc gacgtcttct
     1201 cggcgaccga ggaccgggcc acccgggtcg atttcttcgg cgacgagatc gaatcgatgc
     1261 gctggttttc caccttcacc cagcgatcgc tcgggcaggc ggaatcaatc gagctctccc
     1321 cggcggccga actggacgcc gaacaccgcg agatggccga actgatcatc gccgaggccc
     1381 gtgagaacga gaccgacctc gacctgaccg aggccctgcc gctcgaccac tttcgcgccc
     1441 cgctggacct gatccccgat tcgaccgcgg tgatcctgac cgccgacgag gaaatcgagc
     1501 cggccctgaa ggaccattgg gaggacgtca ccacggcgat gcatgccgat gacgcccggc
     1561 acctctacga ggacgtggcc gggccgctga accagcgggc gtcgctgatt ctcgtccgcc
     1621 cgggcgaggg cgaaggggcg ctccgggcga tgagcgccga gacgccggcc cgcaacctca
     1681 aggaagccga agcggaactg cggaagctgg tcgaggccgg gtaccggacg atcgtcgcct
     1741 tcgactcccg cggcgaggct gaaagggccc gttacggctt tgaccggctc gacacgaaga
     1801 tcgtcgacca ggggccggtt tcgatcgagc ccggggtcag tctggtcgaa tcacggctgg
     1861 cggagggctt cgtttccccc cggaccaagg tcgcggtact gccctttcgc cggctgctca
     1921 accggcgccg ccgggctgac gaaagggctc cggaagccgt tcgcggccgg ctcaccttct
     1981 ccgaactgag ggtcggcgac ttcgtggttc atgaagacca cggggtcgcc cgcttcgccg
     2041 gattcgaaac ccgcgaagtg ggcggcatca cccgcgacta cctctacctc gagtacaagg
     2101 gcgaggaccg ggtctatgtg cccaccgacc agttcgccaa gctctcgcgc tattccggcg
     2161 gggcaggcac ccccaccctt tccgcgctcg ggggcaggaa gtggctgaac atgaaggccc
     2221 gcgcccgcgg cgctgccgac gagatcgccg gcgagctggt caacctctac gccgagcgga
     2281 agacccgcaa gggccacgcc ttcagccctg actcggaatg gcagatggag cttgaagcga
     2341 acttcccgtg gcgcgagacg gccgaccaga tcgaggcgat cgaggcggtc aaggccgaca
     2401 tggaatccga gcagccgatg gaccggctgg tctgcggcga cgtcggctac ggcaagaccg
     2461 aggtggcgat ccgggccgcg gtcaaggccg cctcggacgg caagcaggtg atgatcctgg
     2521 ccccgaccac gatcctcgcc cagcagcatc tcggcacctt ccgcgaacgc ctggccgacc
     2581 tgcctttccg ggtcgacggg gtcagccgcc tgcgcaagcc cgccgaggtc aaacaggtgg
     2641 tcgccgaatg gggcgagggc aaggttgacg tcctgatcgg cacccaccgg ctgctctccc
     2701 gggacgtccg ggcgaaggac ctcggtctga tcgtggttga cgaagaacag cgcttcgggg
     2761 tcaagcagaa ggaactgctg cgccagatga agctcaaggt ggacgtgctt tcgatgtcgg
     2821 cgaccccgat cccgcggacc ctgcagatgt cgatgtccgg gctgagggac atctcggtga
     2881 tcgagacccc gcccgagggt cgccgcccgg tccgcaccta cgtcggcccc tgggacgaac
     2941 agctggtgga acgggcgatc aagcgggaga tcagccgcaa gggccaggtc ttcttcctcc
     3001 acaaccgggt cgaatcactc gacgaggccg ccgagagcct ccgggcgctg gtgcccgggg
     3061 tccggatcca ggtcgcccac ggccagatgg aggatcagga gctcgaggcg gcgatgctga
     3121 acttcctcag gggtgacgcc gactgcctgg tcgccaccac gatcatcgag tcggggattg
     3181 acatccccgc ggccaacacc ctgatcgtcg accgggccga ccacctcggc ctcgcccagg
     3241 cgtaccagat caggggccgg gtcgggcggt cccgcgagcg cgccttcgcc tacctgctct
     3301 acccctcgga agagtcgctc accgcggaag cctcaacccg cctggcgacc ctcgccgacc
     3361 acaccgagct cggctccgga tttcgcattg cgatgcgcga cctcgacatt cgcggggccg
     3421 gcaacctgct cggggacgag cagtccggcc acgtcgcggc ggtcggcttc gagctctact
     3481 gccagttgat cgacgaggcg gtggctgacc tggccgggcg ggaagagtcg gccggggagc
     3541 cggaaccggt tcgcttcgac atcggcgtca acgcctacct gccagccagc tacgtgcctt
     3601 tcgaagcggc caagatcgac atccaccgcc ggatcgccgc tgcccgccgc ccgggagacc
     3661 tgcgccagat caccgacgag ctgatcgacc ggttcggtcc cctgccggaa gacgtcgaga
     3721 acctgatcac gctccagcag gcccggatcg acctcggctc ggccggggtg gagcaggtcc
     3781 agttccgcgg tggcaagctc acggtctccg gaatcgagct cgattccgaa gcggcggctg
     3841 cgatccgcga atcggtgccg ggagcgctct acgaatggcg ttcccatgaa ctctcggtcc
     3901 gaatcgacaa cgaccccgcc aaacggctcg caacggtgtc cgaactgggc cgcgcactgg
     3961 ccgatgtggg tatgctccgc cagtcgtagt ttcagcgacc agccacatca cctttccagg
     4021 aggagccact gcatgagcgg cgcacgcaaa ttcggtctga ttcttttcgg agccatcttg
     4081 gtggttctct ttgcgggtat cgcaattgca caaggcgtca gcaagcccgg cgttgaaagt
     4141 ggcgacatca tcaccgtgca ggacgtgccc gacgggaagg gcagcatcac ggaggaggat
     4201 tacaaccggt ccttcgaaca gacctggaag cgcggcggcc tgcaggcggc accgaaggag
     4261 ggcgacgccc agtacgacca ggtcaaggaa gcggcgatca acgacctgct tgaccaggcc
     4321 tggctggccg gcgtacggcg actcctcttc ctgggcagca gctgcatcta ccccaagcac
     4381 gctgcacaac cgatgagcga ggaagcactg ctgaccggga ccctcgaacc gaccaacgaa
     4441 gcctacgcca tcgccaagat cgccggcatc aagctgtgcg agagctacaa ccgccaatac
     4501 ggcaccgact accgcagcgt catgccgacc aacctgtacg gcccgggtga ccactacgac
     4561 ctgcagaaca gccatgtcat cccggcgatg atccgcaagc tgcacctggc gaaactcgcc
     4621 accgggcacg actgggcggc catcgagcag gacgaggcg
//
LOCUS       DAOQFI010000045         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7809_length_4628_cov_2.782419, whole genome shotgun sequence.
ACCESSION   DAOQFI010000045 DAOQFI010000000
VERSION     DAOQFI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7809_length_4628_cov_2.782419"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..559
                     /locus_tag="PLE93_01410"
     CDS             173..559
                     /locus_tag="PLE93_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNH85734.1"
                     /translation="MIVLDASLLIAHLDAGDAHHEEAEELLAGTGSARLGASTITLAE
                     ILIAPARQGKLEPAWAAVKKLGVEEIPVMADSAPRLAELRARTGLKMPDCCVLEAAGR
                     NNAEVATFDKSLANAAKGIGLTRSTG"
     gene            611..1432
                     /locus_tag="PLE93_01415"
     CDS             611..1432
                     /locus_tag="PLE93_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH85735.1"
                     /translation="MAEPIPDLPSEKQRDQLVTLLRPLLHVTQPKLYGAENVAKAGSL
                     LVGNHTLYAFLDLPFMMAELWSKHQVTVRGLGDNAHYNFPIWRDLLQVGGMVRGTREN
                     VRSLMDAGENVLVFPGGAREVNKRKGEKYQLIWKERIGFAKLAIEYGYPIIPFAAVGA
                     EDAYDIVLDETNPVYARFTGLVKKVTGWPMQPLARGIGPTLIPRPERLYFWFGEPITT
                     SGYAGDNGDSNARQVRDETKAAVEEGIAFLLREQGEDPQRDLSARLLKRDSAKPV"
     gene            complement(1434..1757)
                     /locus_tag="PLE93_01420"
     CDS             complement(1434..1757)
                     /locus_tag="PLE93_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85736.1"
                     /translation="MKPAIYILSDSYVTFTDLAEAVAALEPDAKLPPVMPLTLARAIA
                     AGGEMVAKLTRKPPLIPRGGLHFLSSHAAPDSAKAVEELNWQITPLNEGLEKSLDFFR
                     RKGWL"
     gene            1776..2081
                     /locus_tag="PLE93_01425"
     CDS             1776..2081
                     /locus_tag="PLE93_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85737.1"
                     /translation="MAQISLRISDELAEDIKADAKARKLSVNGYITHILRSASDPEFG
                     GTEGERVRERLRRAGLLEEWLDEPEVERPDPKEIARIRAKAGKGTSLSDLVIEERRH"
     gene            2078..2515
                     /locus_tag="PLE93_01430"
     CDS             2078..2515
                     /locus_tag="PLE93_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH85738.1"
                     /translation="MIFADSSAIAKLYVDEAGSSHMRKFAVLLVSALCRVETTSAIRR
                     KARSGEINESEAEQLVRDFRADLRSKRTDGPELIALPVSTKLLNSAARLVTIHDLRSL
                     DAVQLATALAAREVDPQCDAFACYDERLARVAALHGFTIHSPS"
     gene            2576..2884
                     /locus_tag="PLE93_01435"
     CDS             2576..2884
                     /locus_tag="PLE93_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85739.1"
                     /translation="MPTKLRRIAVTEDVDLASALRSASQALPGLSDAALVKQLAIIGA
                     RSLSTQPPNDHLEHILRIPGAKLPSRPLGSLLKEIADLPKVSSAEVDRILEEERAERL
                     "
     gene            2884..3279
                     /locus_tag="PLE93_01440"
     CDS             2884..3279
                     /locus_tag="PLE93_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH85740.1"
                     /translation="MDAPAYFDTSALTKLILDEEESPRISEFMNTREVSLVSSQLSEV
                     ELLRAVSRSQPGSRPKARELLDRMVLLPITSSILERAAVLDPPSLRSLDAIHLATAVE
                     IKTHLAGVMTYDNRMRQASEGLQLPVVVA"
     gene            complement(3308..3757)
                     /locus_tag="PLE93_01445"
     CDS             complement(3308..3757)
                     /locus_tag="PLE93_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNH85741.1"
                     /translation="MDLQLRIADKTWPVLTKLMGGHARVYRLTRGRIGHHIPGVPPMC
                     LIDHVGAKSGKKRTSPLLYIEDGENIVIVASKGGYEKSPAWFYNLKANPDTTVQIGSE
                     VRPVRARVADEAERKRLWPKAVEVYPSYQQYQDRTNRKIPIVVLEPR"
     gene            3926..4615
                     /locus_tag="PLE93_01450"
     CDS             3926..4615
                     /locus_tag="PLE93_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85742.1"
                     /translation="MNAVRNQSLAIFFGLLFLLALGGQSIAGYQSYNDDEKARAHLAR
                     EAPETIDYPSYLTSPEFSRDVMENWQSEYLQFLLFILATIWLVQRGSPESKRPDESGT
                     ESDRDQKIGPYADPTSPLPARVGGLVTALYSNSLLILMGSIFFAAWFAQSLTGWPAYN
                     EEQVAYGLPTVSWLSYLGTADFWSRTLQNWQSEFLAVGSMAVFAIYLRQRGSPESKPV
                     GESHDSTGIEG"
BASE COUNT          954 a         1380 c         1435 g          859 t
ORIGIN      
        1 ggccggacgt gaaggaccgg aaggatcttc tgatcctgct ggcccggacc ggcatggcga
       61 aggtcagggc tgacgctgaa aaccgtcggc gggtggtgga tgaaaccagc ggcgccctga
      121 ccggtgtgta ccgcgaggac gagctctccg gactccgtga agactggccg gaatgatcgt
      181 ccttgacgcc agcctcctga tcgcgcatct ggacgccggc gacgcgcatc acgaggaagc
      241 ggaagagctt cttgcgggca ccggaagcgc caggctcggc gccagcacaa tcacgctggc
      301 cgagatcctg attgcgcccg ccaggcaggg gaaactcgaa ccggcctggg ccgcggtcaa
      361 gaagctcggg gtcgaggaaa tccctgtgat ggccgactcg gctccgagac tggctgagct
      421 ccgagcgagg accggcctca agatgcccga ctgctgtgtg cttgaagcgg caggacggaa
      481 caacgccgaa gtggccacct tcgacaagtc actcgcaaat gcggcgaaag ggatcggcct
      541 gacgcgatca actggctgaa gctcctccga gcggcctgcg agagatcccg tgaagtagga
      601 gaatcgtcgt atggcggagc cgatccccga cttgccatcg gaaaagcagc gggaccagct
      661 ggtcacattg cttcggcctc tgttgcatgt cacccagccg aagctttatg gggccgagaa
      721 tgtggcgaaa gccgggtcgc tcctggttgg caaccacacc ctttacgcat tcctcgacct
      781 tccgttcatg atggccgaac tctggtcgaa gcatcaggtg accgtccgcg ggctcggtga
      841 caatgcccac tacaacttcc cgatctggcg agatctcctt caggtcgggg gcatggtgag
      901 gggtactcgc gagaacgtcc gctcactcat ggacgccggc gagaacgtcc tggtgtttcc
      961 gggaggagcc agggaggtca acaagcggaa gggagagaag taccagctga tctggaagga
     1021 gcggatcgga ttcgcgaagc tcgcaatcga gtacggatac ccgatcatcc cgttcgcggc
     1081 agtgggcgcc gaggacgcct acgacatagt cctggacgaa accaaccccg tctacgcccg
     1141 gttcacggga ttggtcaaga aggtcaccgg ctggccgatg caacccctcg cgcgtggaat
     1201 cgggccaacc ttgatcccgc gcccggaacg tctctacttc tggttcggcg aaccgatcac
     1261 aacatccggc tatgccggcg acaacggcga ctcgaatgcg cgccaggttc gagatgaaac
     1321 gaaagctgcc gtcgaagaag gaatcgcgtt cctgctgaga gagcaaggcg aggatccgca
     1381 acgggatctc agcgcccggc tcctgaaaag ggactcagcg aagccggtct aagctaaagc
     1441 caacccttcc tccgaaagaa atccaggctc ttctcgaggc cttcgttcaa cggagtgatt
     1501 tgccaattga gttcttcgac ggccttcgct gaatcgggag ccgcgtgcga actcaggaaa
     1561 tggaggccac cacgtggaat gagcgggggc ttgcgggtca gtttggccac catttcgccg
     1621 ccggcggcga tggcacgggc caatgtgaga ggcatgaccg gaggcaattt cgcgtcgggc
     1681 tcgagtgcgg caaccgcttc tgcaagatcg gtgaatgtga cgtagctgtc ggagagaatg
     1741 taaatagcgg gcttcacgag tgctagcatg aaagcatggc tcagatatcg ctgcgaatct
     1801 ccgatgaact ggccgaagac atcaaggctg acgcaaaagc acggaaactt agcgtcaacg
     1861 gctacatcac gcacatcctg cgatctgcga gcgatcccga gttcggcgga acggaaggtg
     1921 agcgtgttcg tgagcggctc cgacgagcgg gattgcttga ggaatggctc gacgaaccag
     1981 aggtggaacg tcccgatccg aaagagattg cccgaatccg ggcaaaagcc ggcaaaggaa
     2041 catcactcag tgacctggtg atcgaagagc gacgccattg atcttcgctg actcatcggc
     2101 aatcgcgaag ctctacgtcg atgaggcagg ttcctctcac atgcggaagt tcgccgtgct
     2161 tctggtgtca gcgctgtgcc gggtagagac aacttctgcg atccggcgaa aggcgcgtag
     2221 cggcgagatc aacgagtccg aagcggaaca actggtccgg gacttccgag cagatctgcg
     2281 atcaaagcgg accgacggac ccgaactcat tgctttgccc gtatccacga agctgctgaa
     2341 ttccgcggca aggctcgtga caatccacga cctcaggtca ctcgacgcgg tccagttggc
     2401 gaccgcgttg gctgccaggg aggtggatcc ccaatgtgac gcgttcgcct gctacgacga
     2461 acggctggcc agggttgccg ctctacacgg cttcaccatc cattctccaa gttgacataa
     2521 gacacattat cgagcattga agaaagtgtg ccaccgacag tgctacactg cgctaatgcc
     2581 caccaaactg aggagaatcg cggtaaccga ggacgttgat ctcgcttctg ccctgcgatc
     2641 ggcaagccag gcgctgccgg gactcagtga cgcggccctg gtcaagcaac tggcgatcat
     2701 tggagcaagg agtctctcca cgcagccccc gaacgaccat cttgagcaca tcctcaggat
     2761 tcccggggcg aaattgcctt ctcggccgct tggttcgctc ctgaaggaaa tagcggatct
     2821 gcccaaggta agttccgccg aggttgatcg gatcctcgag gaagaacggg cagagcggct
     2881 ttgatggacg cccccgccta tttcgacacc tcggcgctga cgaagctgat tctcgacgag
     2941 gaagagagtc ctcggatcag cgaatttatg aacactcgcg aagtgagcct cgtctcgagt
     3001 caattgagcg aagttgagct cttgcgtgcg gtttcacggt cgcagccggg cagccggccg
     3061 aaagccaggg aattgctgga ccgcatggtt cttctgccga ttactagctc gatcctggaa
     3121 agagccgcgg ttctggatcc accttctctg agaagcctgg acgcgatcca cctggcgacc
     3181 gccgtcgaga tcaagactca tctggcaggc gtaatgactt atgacaaccg gatgcgtcag
     3241 gcatctgaag gcctgcaact cccggtcgtc gtcgcatgac cgccgatcaa aagggaactc
     3301 tcggtgatta gcgtggctcg aggacgacga tcgggatctt ccggttggtg cggtcctggt
     3361 actgctggta tgacgggtag acctcgaccg ctttcggcca gaggcgtttg cgttcggctt
     3421 cgtcggcgac gcgggcccgg accggacgga cttcgctgcc gatctggacc gtagtgtccg
     3481 ggttggcctt gaggttgtag aaccaggccg ggctcttctc gtagccgccc ttcgaggcga
     3541 cgatcacgat gttctcgcca tcttcgatgt agagcagcgg gctcgtgcgt ttctttccgc
     3601 tcttcgcgcc gacgtgatcg atcaggcaca tcggaggcac accgggaatg tgatgaccga
     3661 ttcgtcccct ggtgaggcgg taaacgcgag cgtgaccgcc catcagcttc gtgagcactg
     3721 gccacgtctt gtcggcaatt cggagctgga ggtccattgc ctcaacctat cgtggcccga
     3781 cgacacgggc tcccgcttct cggtcctgcc ggtacgaaaa gggcgccggc gggtacacgg
     3841 cgggtatgag ggtcgtcttc ttcgtgtatc tgatcggggt gattgcgggc cttgcgtttg
     3901 cgatctcggt gggagcaaac agcgcatgaa cgcggtccgg aaccagagcc tcgcgatctt
     3961 cttcggcctg ctctttctgc ttgcgctcgg aggccagtcg atcgccggct accagagcta
     4021 caacgatgac gagaaggcgc gtgcccacct ggcgcgcgag gctcccgaaa ccatcgacta
     4081 ccccagctac ctgacctcac cggaattctc gcgagacgtg atggaaaact ggcagtcgga
     4141 atacctgcag ttcctgctct tcatcctcgc aacgatctgg ctggtccagc ggggctcgcc
     4201 tgaatcgaag cgacccgacg aaagcggcac cgagagtgac cgcgaccaga aaatcggacc
     4261 gtacgcagac ccgacctctc ccctccccgc ccgggtcggc ggtctcgtca ccgctctcta
     4321 ctcgaactcg ctactgatcc tgatgggatc gatctttttc gcggcctggt tcgcccagtc
     4381 acttaccggc tggcctgcct acaacgaaga acaggtcgcc tacggcctgc ccaccgtcag
     4441 ctggttgagc tacctcggaa ccgctgactt ctggagccgc acgctccaga actggcaatc
     4501 ggaattcctc gcggtcggtt cgatggccgt cttcgcgatc tacctgagac agcgaggctc
     4561 gccggaatcc aagccggtcg gcgagagcca cgattcgacc gggatcgagg gttgatgccc
     4621 gatcacct
//
LOCUS       DAOQFI010000046         4581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7933_length_4581_cov_3.667256, whole genome shotgun sequence.
ACCESSION   DAOQFI010000046 DAOQFI010000000
VERSION     DAOQFI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7933_length_4581_cov_3.667256"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLE93_01455"
     CDS             complement(<1..517)
                     /locus_tag="PLE93_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH85743.1"
                     /translation="MGRLIYGSMSITLDGYFADADGGIGWTVPEDEVHRLANQQVRGH
                     AAVIMGRGLYEMMVPYWYDLLGSGEGGEIEQEFAMVWDEIPKFVASRTLTAVHETCQL
                     IEGDAVDWIARKKAETDGDIEVSGIDLASQVEAAGLIDVYRFNLLPVVLGGGKPFFPG
                     NGTRRDLELTDL"
     gene            complement(531..1283)
                     /gene="cofE"
                     /locus_tag="PLE93_01460"
     CDS             complement(531..1283)
                     /gene="cofE"
                     /locus_tag="PLE93_01460"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNH85744.1"
                     /translation="MDAAMIIPVEGLPEVTETFNIGKAIAERVQMTDGDIVAVSQKIV
                     SKSEGRVVRLDETDPGPDAVSLAARTGKDPRLVQHVLNESRNLIRVDEQRSVLITETH
                     HGYICANAGIDQSNTAGENQAILLPLDPDGSARRIRVELETASGVKAAVVVTDSFGRA
                     WRHGQVETAIGCAGIDPLDDWRGQEDQRGRELTATVIAVADQIAAASDLVRTKTDGVP
                     AVVLRGLDHFVTEDDGPGCAAQLREPGDDLFR"
     gene            complement(1284..1937)
                     /gene="cofC"
                     /locus_tag="PLE93_01465"
     CDS             complement(1284..1937)
                     /gene="cofC"
                     /locus_tag="PLE93_01465"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNH85745.1"
                     /translation="MNSVAVITAKRFPAAKQRMAGGVPEERRLALVEAMLGDVLEAVT
                     GARQVNFTVVVTGEVAAADLAAGHGAEVIHDPEDAGHIGAAELGIERAIELGAECVIL
                     LPGDCPLLEAREIDSLLTGIPEKFVTVVPDRHGTGTNALVLRPPDAIKPAFGEGSCQR
                     HLRLARDAGLPHDTEEVAGLALDLDTPADIVALTTKIEMVRATGGNPAPRTAAVLGI"
     gene            complement(1934..2860)
                     /gene="cofD"
                     /locus_tag="PLE93_01470"
     CDS             complement(1934..2860)
                     /gene="cofD"
                     /locus_tag="PLE93_01470"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNH85746.1"
                     /translation="MKVALLAGGTGGAKLAAGMQAVVGDGLTVIANTGDDIETLGVYV
                     SPDPDLVTYWLSGQVDEVRGWGIKDDGFDVFQRMARFGAPDWFGLSNLDLAACLYRKD
                     FMASGGRLTDAQAQITRGLGVRATVLPMSDQPVRTRIKSSGEWRGLQEYLIIEGGQTE
                     VEGVQLDGIEEAEPTPELIEAISSADLIVIGPSNPVISIGPILAVPGIRQAMIEAAAP
                     VVAVSPYVAGKVIKGPTDLFMKAIGRPTTATGVAGLYQGLINAMITDADDPDPPPTEV
                     RSFSTATLMHSAESRATVARAVIKVGETLAGS"
     gene            complement(2860..3555)
                     /gene="npdG"
                     /locus_tag="PLE93_01475"
     CDS             complement(2860..3555)
                     /gene="npdG"
                     /locus_tag="PLE93_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNH85747.1"
                     /translation="MSENSESPVIPVIGGTGALGSGLAKRWAAGGARVVIGSRDAGRA
                     AEAAAKIAEETGGTVEGMSNEDAAKAADIVFLTIPFRNQSEYLTNLKGSLTEGQILVD
                     CSVPLAAAISGKATRTIGVWQGSAAEQAQEMVPDGVTVVSALHTVSAPGLADLSHDLD
                     EDVPVCGDRKADKARVAELISRIEGLRPVNAGALEISRIVEQITALMISVNIRYKTHA
                     GLKFTNLPEGETF"
     gene            3601..3972
                     /locus_tag="PLE93_01480"
     CDS             3601..3972
                     /locus_tag="PLE93_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85748.1"
                     /translation="MSIAEKTGTEREENFTVEGMLMTDVGGLLETGVLSTPGMIGMME
                     RNCALLVQEFLPEGSATVGFEVNVKHVSGAGEGAECTAWGRLDEVIDERKFRFSVEVR
                     EGDRVLGVGTHERRAITFNVA"
     gene            3984..>4581
                     /locus_tag="PLE93_01485"
     CDS             3984..>4581
                     /locus_tag="PLE93_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNH85749.1"
                     /translation="MAFSEENFPKAVRIREVGPRDGFQNEPEVIPTEEKIRLINMLGQ
                     TGLRRIEVTSFVRPDVIPQLSDGVEVLNGIDRPEGVSYSVLIPNRRGLENALDLRDRF
                     DETNFFLSASETHNRKNVNRSVEESMSGLKETIPVAVEAGLRCEGVISTSFGCPYEGD
                     VPVENVLALALRLARAGCVEVGFGDTTGMANPVQVRRFF"
BASE COUNT          743 a         1504 c         1484 g          850 t
ORIGIN      
        1 gcagatcggt cagttcaagg tcccgccggg ttccgttccc ggggaagaac ggcttgccgc
       61 cgccgaggac gacgggcagg agattgaagc ggtagacgtc gatcagcccg gctgcctcga
      121 cctgggaggc gaggtcgatg ccgctcacct cgatgtcgcc gtcggtctcg gccttcttgc
      181 gggcaatcca gtccaccgcg tcgccctcga tcagctggca ggtctcgtga accgcggtca
      241 gggtgcgcga ggcaacgaac ttcggtatct cgtcccagac catggcgaac tcctgctcga
      301 tttcaccgcc ctcgccggac cccaggaggt cgtaccagta cgggaccatc atctcgtaga
      361 ggccgcggcc catgatgacg gccgcgtgac ctcgaacctg ctggttggcg agccggtgga
      421 cttcatcctc cggcacggtc cagccgatac cgccgtcggc gtcagcaaag tagccatcca
      481 gcgtgatcga catcgatccg taaatgagtc gtcccatgga ctcagggtaa ctagcggaag
      541 aggtcgtcac cgggttcgcg cagctgggcc gcgcagcccg ggccgtcgtc ttccgtgacg
      601 aaatggtcga gccctctcag gaccacggct ggcacgccgt cggtcttggt ccggaccagg
      661 tcggaagcgg cggcgatctg gtcggccacc gcgattacgg ttgcggtcag ctcccggccc
      721 cgctggtcct cctggccgcg ccagtcgtcc agcgggtcga ttccggcgca gccgatcgcg
      781 gtctcgacct ggccgtgacg ccaggcccgg ccgaagctgt cggtgaccac caccgccgcc
      841 ttgacccccg atgcggtttc cagctcaacc cggatccgcc gggccgaacc gtccgggtcc
      901 agcggcagca ggatcgcttg gttctcgccg gccgtattcg actggtcgat gccggcgttt
      961 gcgcagatgt agccgtgatg ggtctcggtg atcaggacgc tccgctgctc gtcaacccgg
     1021 atcaggttcc ggctctcgtt cagcacgtgc tgcaccagcc tcggatcctt gccggtccgg
     1081 gcggccagcg agacggcatc cggtccgggg tcggtctcat ccaacctgac gacgcgacct
     1141 tccgacttgg agacgatctt ctgcgaaacc gcgacgatgt cgccgtcggt catctgaacc
     1201 cgctcggcaa tcgccttgcc gatgttgaag gtttcggtca cctctggcag accctcgacc
     1261 gggatgatca tcgcggcgtc catctagatg cccaggacgg cggcggtccg cggggccgga
     1321 tttcccccgg tggcacgaac catctcgatc tttgtggtca gggcaacgat gtcagctgga
     1381 gtatcgagat ccagggccag tcccgcaacc tcttccgtgt cgtgcggcag ccccgcatcc
     1441 cgggcgaggc ggaggtgtct ctggcagctt ccttcgccga aagccggttt gatcgcatca
     1501 ggaggacgga gcacaagggc gttcgtaccc gttccgtgcc gatctggaac aaccgtgacg
     1561 aatttctccg ggatgccggt cagcaggctg tcgatttcac gggcctcaag cagcggacaa
     1621 tcgcccggca ggaggatcac gcactcggct ccgagttcga tcgcccgctc gatcccgagt
     1681 tcggcggcac cgatgtgacc ggcgtcctcc gggtcgtgga tcacctcggc gccatggccg
     1741 gccgccaggt ctgccgcggc aacctcgccg gtgacgacta ccgtgaagtt gacctgcctt
     1801 gccccggtca ctgcctcgag cacgtcgccg agcatcgcct cgaccagggc caggcgcctc
     1861 tcttcgggca ctccgccggc catgcgctgt ttggccgcgg gaaatctctt tgccgtgatt
     1921 accgcgaccg agttcaagat ccggccaggg tctcgccgac cttgatcacg gcccgggcga
     1981 ccgttgcccg gctctcggcg ctgtgcatca gggtcgccgt ggagaaggaa cggacctcgg
     2041 tcggcggcgg gtccggatca tcggcgtcgg tgatcatcgc gttgatcaga ccctgataga
     2101 gaccggccac tccggtcgcc gtggttggcc ggccaatcgc cttcatgaag aggtcggtcg
     2161 ggcccttgat caccttgccg gccacatagg ggctgaccgc caccaccggt gccgcggctt
     2221 cgatcatcgc ctggcgtatt cccggcacgg caaggatcgg cccgatgctg atcaccgggt
     2281 tggaaggtcc gatcacgatc aggtccgccg aactgatcgc ctcgatcagc tcgggggttg
     2341 gttcggcttc ctcgattccg tcgagctgga cgccttcaac ctcggtctgg ccgccctcga
     2401 tgatcagata ctcctgcagc ccgcgccact ccccgctgct tttgatccgc gtgcggactg
     2461 gctggtcaga catcggcagc acggtggccc tgactccgag ccccctcgtg atctgcgcct
     2521 gggcatccgt caggcgtccg cctgaggcca tgaagtcctt gcggtagagg caggcggcga
     2581 ggtcgaggtt ggagagtccg aaccagtcgg gggctccgaa tcgtgccatc cgctggaaga
     2641 cgtcgaaacc atcgtccttg atgccccagc cccgcacttc gtccacctgt ccggacagcc
     2701 agtaggtgac caggtccggg tcgggtgaga cgtagacgcc gagggtctcg atgtcgtcgc
     2761 cggtgttggc gatcacggtc agcccgtcgc cgaccaccgc ctgcatcccg gcagcaagct
     2821 tggccccgcc agtgcccccg gccagcaggg cgaccttcat cagaaagtct cgccctcggg
     2881 gaggttcgtg aacttgagcc cggcgtgggt cttgtacctg atgttgaccg agatcatcag
     2941 ggcggtgatc tgttcgacga tccgcgagat ctcgagggcg ccggcattta ccgggcgcag
     3001 gccttcgatc ctcgaaatca gttcggcgac cctcgccttg tcggccttcc tgtcgccaca
     3061 gaccggcacg tcctcgtcaa ggtcatgtga caggtcggcg agtccggggg cgctgacggt
     3121 gtggagagcg gagaccacag tcaccccgtc ggggaccatc tcctgcgcct gttcggccgc
     3181 ggagccttgc cagacgccga tcgttcgggt cgccttgcca ctgatcgctg cggcaagagg
     3241 taccgagcag tcgaccagga tctgaccttc ggtcagcgat cccttgagat tggtcaggta
     3301 ctcggactgg ttccggaagg gaatggtgag aaagacgatg tcggcggctt tcgcggcatc
     3361 ctcgttggac atgccttcga ccgttccacc cgtctcttcg gcgatctttg ccgcggcctc
     3421 tgccgcacgg ccggcgtcac gagagccgat cacgaccctg gcgcctccgg cagcccagcg
     3481 tttggcgagg ccggagccga gcgctcccgt tccgccgatt acagggatga caggtgattc
     3541 gctgttctcg ctcacggggt cgcagtctag gtcaagccgc agcccaacta ttcttggccc
     3601 atgtcgattg ccgagaagac cgggaccgag cgcgaggaaa acttcaccgt cgaggggatg
     3661 ctgatgaccg acgtcggcgg tcttctcgag accggggtgc tctcgacccc cgggatgatc
     3721 ggaatgatgg agcggaactg tgcccttctg gtccaggagt tcctgcccga aggatcggcc
     3781 acggtcggct tcgaggtgaa cgtcaagcat gtctccggag cgggtgaagg ggccgagtgc
     3841 accgcctggg gtcgcctcga cgaggtaatc gacgagcgca agttccgctt ctcggttgag
     3901 gtccgcgaag gcgacagggt tctcggggtt ggaacccacg aacgtcgcgc gatcaccttc
     3961 aatgtcgcct gagcggggaa cccatggcct tcagcgaaga gaacttcccc aaggcggtca
     4021 ggatccgcga ggtaggaccg cgtgacggct tccagaacga gcccgaagta atcccgacag
     4081 aggagaagat ccggctgatc aacatgctcg gccagactgg cctgaggcgg atcgaggtca
     4141 cttccttcgt tcggcccgac gtgatcccgc agctctcgga tggggttgaa gtcctgaacg
     4201 gcatcgatcg tcccgaaggt gtctcctact cggtactgat cccgaaccgt cgtgggctcg
     4261 agaacgcact cgatcttcgc gaccgcttcg acgagaccaa cttcttcctc tccgcttccg
     4321 agacacacaa ccgcaagaac gtcaatcgca gcgtcgagga gtcgatgagc ggtctgaaag
     4381 agacgatccc ggtggctgtg gaagccgggc tccgctgcga aggggtgatt tccacttcct
     4441 tcggttgtcc ctacgaaggc gatgtgccgg tcgagaacgt gctcgcgctg gccctgcggc
     4501 tggccagggc gggctgcgtg gaagttgggt tcggggacac gaccggcatg gccaacccgg
     4561 ttcaggtccg gcgcttcttc t
//
LOCUS       DAOQFI010000047         4572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_7966_length_4572_cov_2.911003, whole genome shotgun sequence.
ACCESSION   DAOQFI010000047 DAOQFI010000000
VERSION     DAOQFI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7966_length_4572_cov_2.911003"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLE93_01490"
     CDS             <1..382
                     /locus_tag="PLE93_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH85750.1"
                     /translation="GGGSGYHAAVLARLARRVISIERQPELVEAARRSLAAAGVVNVE
                     MICGDGSVGCPGEAPFDAINVAAAVRGEVPEALPGQLAEGGRMVVPVGRRHQWLELWR
                     RREGRLSRERMVAVAFVPLVEEGD"
     gene            410..1099
                     /locus_tag="PLE93_01495"
     CDS             410..1099
                     /locus_tag="PLE93_01495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH85751.1"
                     /translation="MARATSVFEKQAATYRGPREKLIPQMERFYGMVPEAIGLAEPET
                     GREGQPLRILDLGAGTGMLSAVVAEAWPDAAFTLFDFSPRMLEQAKESLGEAVRVLTG
                     DMYEGIPAGPFDAVISALAIHHLTDEGKRSVYEAVFGELRPGGIFVNAEHVLGETPAL
                     QQHYAKWHRRQAFEKGLTESEWADTVERMSHDHLTPLSTQLGWLEEIGFTDVDCLFKD
                     HGFAVLFGRRP"
     gene            complement(1116..1916)
                     /locus_tag="PLE93_01500"
     CDS             complement(1116..1916)
                     /locus_tag="PLE93_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH85752.1"
                     /translation="MTPGFTPFHHGGSGPPLVLIHGFTDTWRTWDLVIPRLERDFEVL
                     AVTMPGHAGGPAIEGKLTVDTLPSGIEAAMEAAGFTHAHLAGNSLGGWVALQLAARGR
                     ALSVTGLAPAGGWSAEFQNQWALDYFITQQDLVKKAAPYADQIASTPEGRRQATRFIV
                     SDIGLITPEMVVHQLIGAASCPAAMPMVELAREQGFELDAEQIECPVRFVWGTADLLL
                     RWPETAVRFREEWLPAAEFIELDGIGHCPQLEVPAEVAELIAGTARLA"
     gene            complement(1929..2429)
                     /locus_tag="PLE93_01505"
     CDS             complement(1929..2429)
                     /locus_tag="PLE93_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH85753.1"
                     /translation="MARLRLLFTGALLAFGLQTLIRRLLLTQFRRSVEALNQGDYGPL
                     LDGFADDAVLHFNDAPHRWAGEHRGRDQIETFLKDFVAAGLQGEILDLWTSGPPWAMT
                     AVARFDDRSTGPDGQELYSNQTMIMVRTRWGKVTEQRDFYVDTTSMIDFDRRLTELGI
                     LPVADG"
     gene            2454..3140
                     /locus_tag="PLE93_01510"
     CDS             2454..3140
                     /locus_tag="PLE93_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNH85754.1"
                     /translation="MPGSGTETVSRRVHDELRRQIVEGELAPGDRIPSERVLAEDFGV
                     NRHAVREALKGLQAAGLIRITHGGATRVLDWRDAGGLEVLLDLGGDPLEPPAEIVRSV
                     VEMRASIGVDAARLCASRASQSERDQVEQLGESAAAMIGTGDEAGLDEAFAAFWLAIV
                     AGSGNIAYRLSLNSLLKAMLTFGDVADRIRPADPRIITRLGNAIGAGDPETAAEVAGH
                     MLRSDIDRVA"
     gene            3210..4151
                     /locus_tag="PLE93_01515"
     CDS             3210..4151
                     /locus_tag="PLE93_01515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH85755.1"
                     /translation="MGDLILYAIPFFFLAMGLEYLTLRHAAHEHGEAQAANPDAPIGF
                     EAKDTATSLSMGVGHLFIAGAWKLVVLVIYAAIYEVSPIQLSPSDWWTWVLLFFADDL
                     AYYCFHRSHHRIRLFWAMHVVHHSSEHYNFSTALRQDWSPFSSIFFWMPSALLFPPWM
                     IYLAFSWSLLYQFLLHTEAIDRLPRPIEFIFNTPSHHRVHHGSQEQYLDKNYGGILII
                     WDRMFGSFEPEEERVRYGLTKNIKTFHPVKVAYGEYMNIWRDVRAADSWRDRFGYAFK
                     GPGWKPEPIAADDERIATDSHRRSDGTDRDRRLAAAD"
     gene            4181..>4572
                     /locus_tag="PLE93_01520"
     CDS             4181..>4572
                     /locus_tag="PLE93_01520"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HNH85756.1"
                     /translation="MAADSSAKSLPAPGWYPVPGRDRDTTVERHWDGEVWTDEVRTAP
                     VGSSLATYRRHFWGFVRNGNWKLLLVLILTLAGAAALWGTDRKADLVGGIQILAVPLA
                     AAATFVTMVAFLRFIGRRVGFDRIPVETR"
BASE COUNT          764 a         1517 c         1520 g          771 t
ORIGIN      
        1 gggcggtggc tcgggctatc acgcagcggt gctggcgagg ctcgcccgcc gggtgatctc
       61 aatcgagcgc cagccggaac tcgtcgaagc ggctcgccgg tccctggctg cggccggcgt
      121 cgtgaacgtg gagatgatct gcggtgacgg gtcggtcggc tgcccggggg aagcgccttt
      181 tgacgcgatc aacgtggccg cggcagttcg gggcgaggtg ccggaggcgc tcccggggca
      241 actcgccgag ggcgggcgga tggtggtgcc ggtcgggcgc cgacatcagt ggctggagct
      301 ctggcgaagg cgggaaggcc ggctctcccg cgagcgcatg gttgccgtcg ccttcgtccc
      361 cctggtggaa gaaggggact gacccggtcg aggatctagg atcagaccca tggccagggc
      421 gacctcggtt ttcgagaagc aggccgcgac ctatcgcggc ccaagggaga agctgattcc
      481 ccagatggag cgcttctacg ggatggtgcc ggaagcgatc ggactggccg aacccgaaac
      541 cgggagggag ggccagccgc tcaggatcct cgacctcggc gcgggaaccg gaatgctctc
      601 cgcggtggtc gcggaagcct ggccggatgc ggcgttcacg ctctttgact tttccccgcg
      661 gatgctggaa caggcgaagg aaagcctggg agaagcggtc cgggtgctga ccggggacat
      721 gtacgaggga atcccggccg ggccctttga cgcggtgatc tcggccctcg ccattcacca
      781 cctgaccgac gagggaaagc ggtcggtcta cgaggcggtt ttcggggagt tgagaccggg
      841 cggcatcttc gtcaacgccg agcacgtcct cggcgagacc ccggccctgc agcagcacta
      901 cgcaaaatgg caccgccgcc aggccttcga gaagggcctg accgaatccg aatgggccga
      961 caccgtcgaa cgcatgagcc acgaccacct gaccccgctt tcgacccagc ttggatggct
     1021 cgaagagatc ggtttcaccg acgtcgactg tctcttcaag gaccacggct tcgccgtgct
     1081 cttcggtcgc cggccctgaa cgaccgacgc gcggctcagg ccaggcgggc ggtcccggcg
     1141 atcagctcgg cgacctcggc cggcacctcc agctgggggc agtggccgat cccgtcgagt
     1201 tcgatgaact cggccgccgg cagccactcc tcgcgaaagc ggaccgctgt ctccggccag
     1261 cgaagcagca ggtcagccgt gccccagacg aaccgcaccg ggcactcgat ctgctccgcg
     1321 tccagctcga agccctgttc gcgggccagt tcgaccatcg gcatcgcggc cgggcagctg
     1381 gcggctccga tcaactggtg aaccaccatt tccggcgtga tcaggccgat gtcggagacg
     1441 atgaagcggg tcgcctggcg ccggccttcc ggggtcgacg cgatctgatc ggcgtagggg
     1501 gccgccttct tgaccaggtc ctgctgggtg atgaagtaat cgagcgccca ctggttctgg
     1561 aactccgccg accagccccc ggccggggcg aggccggtga ccgaaagcgc gcgaccgcgc
     1621 gccgcgagct gcagggcgac ccagccgccg agcgagtttc cggccaggtg cgcatgagtg
     1681 aagccggctg cttccatcgc ggcttcgatt cccgacggga gggtgtcgac ggtgagcttc
     1741 ccttcgatcg ccgggcctcc ggcatgcccg ggcatggtca ccgccagcac ctcgaagtcg
     1801 cgttcaagcc gggggatgac cagatcccag gttcgccagg tgtcggtgaa accgtggatc
     1861 aggaccagcg gcggtcccga tccgccgtga tggaaggggg tgaacccggg ggtcatgccg
     1921 gaagcatctc agccgtcggc gaccggcagg attcccagct cggtcagcct gcggtcgaaa
     1981 tcgatcatgc tggtcgtgtc aacgtagaag tcgcgctgct cagtcacctt gccccatctg
     2041 gtccggacca tgatcatggt ctggttcgag tagagctcct gaccgtccgg tccggtcgat
     2101 cggtcgtcaa agcgggccac ggcggtcatc gcccagggtg ggccggaagt ccacaggtcg
     2161 agaatttctc cctggagccc ggccgctacg aaatccttga ggaaggtctc gatctgatcg
     2221 cgcccccgat gctcgccggc ccagcgatgt ggcgcgtcat tgaaatgaag cacggcgtca
     2281 tcggcgaaac cgtccagcag cgggccgtag tccccctggt tgagggcctc cacgctccgc
     2341 cggaattgcg tcagcagcag cctgcgaatc agggtctgaa gaccgaacgc gaggagcgca
     2401 ccggtgaaca gcagtctcaa tcgggccata gggagatggt aaaacctcct gcaatgcccg
     2461 gttccggcac agagaccgtt tcccgtcgcg tccacgacga gctgcgccgc cagatcgtcg
     2521 agggtgaact tgcgcccggg gatcgcatcc cgtccgagcg ggtcctcgcc gaagacttcg
     2581 gcgtgaatcg ccatgccgtt cgcgaggcgc tcaagggtct ccaggcagcc gggctgatca
     2641 ggatcaccca cggcggtgcg accagggtgc tggactggcg tgacgccggc gggctcgagg
     2701 ttctgctcga cctcggtggc gacccgctcg aaccaccggc cgagatcgtc aggtccgtgg
     2761 tcgagatgag agcctcgatc ggggttgacg cggcgaggct ctgcgccagc cgtgcgagcc
     2821 agtcggaacg cgatcaggtc gaacaactgg gcgaaagcgc cgccgcaatg atcggaaccg
     2881 gagacgaggc agggctcgat gaagccttcg ccgccttctg gctggcgatc gtcgccgggt
     2941 ccggcaatat cgcctaccgg ctttcactca actcgctgct caaggcgatg ctcaccttcg
     3001 gagacgtggc cgaccggatc cgcccggccg acccccggat catcacccgg ctcggcaacg
     3061 cgatcggcgc cggcgacccc gagacggcgg ccgaggtcgc tggccacatg ctgcgcagcg
     3121 acatcgaccg cgtcgcctga accggatccc ggctgcggcc gatccgcccc ttgcaaccgt
     3181 ctgggtgaac tggtataacc agttcggcaa tgggcgatct gattctttac gcgatcccgt
     3241 tcttcttcct tgcgatgggt ctggagtacc tgaccctgcg ccacgcggcg catgagcacg
     3301 gagaagcgca ggccgcaaac ccggacgcgc cgatcggctt cgaagcgaag gacacggcca
     3361 ccagtctcag catgggggtc ggccacctgt tcatcgccgg cgcctggaag ctggtcgttc
     3421 tcgtcatcta cgccgcgatc tacgaagtca gcccgatcca gctttcacct tcggactggt
     3481 ggacctgggt gctgctcttc ttcgccgacg atctcgccta ctactgcttc caccgcagcc
     3541 accaccgcat ccggctcttc tgggcgatgc atgtggttca ccactcctcc gagcattaca
     3601 acttctcgac cgcgcttcgt caggactggt cgccgttcag ctcgatcttc ttctggatgc
     3661 cttcggccct gcttttcccg ccgtggatga tctacctcgc gttttcatgg agcctgctct
     3721 accagttcct gctccacacc gaggcgatcg accggctgcc ccggccgatc gagttcatct
     3781 tcaacacgcc cagccaccac cgggtccacc acggctccca ggagcagtac ctcgacaaga
     3841 actacggcgg aatcctgatc atctgggacc gcatgttcgg cagcttcgaa ccggaggaag
     3901 aacgggttcg ctacggcctg accaaaaaca tcaagacgtt ccatccggtc aaggtcgcct
     3961 acggcgaata catgaacatc tggcgtgacg tgcgggccgc cgacagctgg cgggaccggt
     4021 tcggctacgc cttcaagggt ccgggctgga aacctgagcc gatcgccgca gacgatgagc
     4081 ggatcgcgac cgattcacat cggcgctccg acggcaccga tcgggatcga cggctggctg
     4141 ctgctgattg atccgctggt tccggggtag ctaccgtggg atggctgccg actcttccgc
     4201 gaagagcctt cccgcacctg gctggtaccc cgtcccgggc cgcgatcggg acacgaccgt
     4261 cgagcgccac tgggacggcg aagtctggac cgacgaggtc aggaccgctc cggtcggttc
     4321 aagtctcgct acctaccgga ggcacttctg gggcttcgtc cggaacggca actggaagct
     4381 cctgctggtc ctgatcctga cccttgccgg cgcggcggcg ttgtggggta ccgaccgcaa
     4441 ggccgatttg gtcggcggca tccagatcct ggccgtgccc ctggccgcgg cggcgacctt
     4501 cgtgacgatg gtcgccttcc tgagattcat cggccgccgg gtcggtttcg accggatccc
     4561 ggtcgaaacg cg
//
LOCUS       DAOQFI010000048         4552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8048_length_4552_cov_3.225039, whole genome shotgun sequence.
ACCESSION   DAOQFI010000048 DAOQFI010000000
VERSION     DAOQFI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8048_length_4552_cov_3.225039"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLE93_01525"
     CDS             complement(<1..499)
                     /locus_tag="PLE93_01525"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNH85757.1"
                     /translation="MLLAIDVGNTQTHLGAFEGNELTRHWRFQTRAGATSDELAERIS
                     GLMELRGTGFDDLDGLVVSSVVPPLGSEYTRLAQQYLDVGCLLVGPGIRTGMSIEIDN
                     PAEVGADRLVNAIAAYDEVGDVCVGVDFGTGINFDAVSADGAYLGGASAPGVEISLTA
                     LTERAA"
     gene            543..1310
                     /locus_tag="PLE93_01530"
     CDS             543..1310
                     /locus_tag="PLE93_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNH85758.1"
                     /translation="MSKATLFSLPGSHPAVSVKLMLDYKGIDFKTIDLMPLMHKAVVR
                     AAGFPGTTVPALKFNGEKLQGSVDIARALDRIVPEPPLLPADEDARKQVLMAEAFGEA
                     ELQHAVRQILWNAVRRQPKSIATFLEGSSLPLPPKVAAYTSGPIIWGEYKVNESTDEN
                     VKANLIALPEWLDRLDAWVADGTLGGATPNAADFQIATSIRLAATLQDLRPFLENRPI
                     YEAAVRTAPVYAGDVPPGLPTEWLAPLRENAAAPVSD"
     gene            complement(1470..2681)
                     /locus_tag="PLE93_01535"
     CDS             complement(1470..2681)
                     /locus_tag="PLE93_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH85759.1"
                     /translation="MSDAEKIVIVGASLAGARAAEGAREAGFEGEIVLIGDEPERPYE
                     RPPLSKDYLRGEKDDLAWVHEESFYAEHDIDLRTSTFVSGIDPSAGTVLVEGGDPEPY
                     DRLLLATGCEPRRIDIPGAELDGIHYLRSAGDSAELGERFREGTRLVVVGAGWIGSEV
                     AASARQKGCEVTLVEPQEVPLQSVLGTEVGGVYAEIHLEQGVDFKSGTGVEAFLGEGS
                     VSKVRTTAGEIEADLVVVGVGAVPRTGLAEAAGLELNNGVPVDAGLRTEAEGIYAAGD
                     IANQFHPFFNDRIRVEHWANARRQGFAAGRSMAGAEVIYDDLPYFFSDQYDVGMEYVG
                     FASDWDEVVIRGSKENREFIAFWLKDGRIQAGMNVNIWDVSETIGELIKSKRQIDLVA
                     LADPDTDLATI"
     gene            2797..3369
                     /locus_tag="PLE93_01540"
     CDS             2797..3369
                     /locus_tag="PLE93_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85760.1"
                     /translation="MRSEERFPIRRSVTLIVAAFTLTGSAFAVSGCGGSGEEPKQWPN
                     DAGYPFDEVKLAADGVPRTAKLTADQKAEILRIARNDPLVKELLEGHEVEWRKPYPWS
                     RPNMELLGGGVDVHFAEPVDLSGATIPAIENIEELYGKQGEEGVILEEGEPRVEPEIG
                     TTRSPYTDVRALSLLVDLEQGRVAQVLQIG"
     gene            3371..4246
                     /locus_tag="PLE93_01545"
     CDS             3371..4246
                     /locus_tag="PLE93_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNH85761.1"
                     /translation="MSFSQSVSEAVDPDVVSCLPGGGGDINRGWRIETAEGDRFFAKS
                     RPGGSVAEFVNEAAGLRWLGEVGALPVPEVIAVVEDETEPGLILDWVEPGPRADEAEL
                     GRGLALIHQAGADGFDRLAPGATGNEIRFGEAAMPLNPVPAGEGFAGVYAARLRHLGG
                     EAYEHGALGSSDRDVIEQVADRADGLAGPVEPPARVHGDLWSGNLLWSEGRPWLIDPA
                     AHGGHCEIDLAMLELFGSPGPAFYSAYEEVAPLADGYRERIGFWQLQPLLVHAVLFGG
                     GYGRSAAVTARACLG"
     gene            4357..>4552
                     /locus_tag="PLE93_01550"
     CDS             4357..>4552
                     /locus_tag="PLE93_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNH85762.1"
                     /translation="MNKWRYLLVSVLAALVAGLTIPAASSAWAPAASATVHPGVQTVT
                     EGAQCTSNFVFQNGSDVYLGQ"
BASE COUNT          750 a         1552 c         1466 g          784 t
ORIGIN      
        1 gcgccgcccg ttcggtcagc gcggtcagcg agatctcgac accgggggcg ctcgcgccac
       61 cgaggtaggc accgtcagca gagaccgcgt cgaaattgat gccggtgccg aaatcaaccc
      121 ccacgcagac atcgcccacc tcgtcgtagg cggcgatcgc gttgaccagc cggtccgcac
      181 cgacttcggc ggggttgtcg atttcaattg acattccggt gcggataccc ggtccgacca
      241 ggagacaacc cacatcgagg tattgctggg ccagcctggt gtactcggag ccgagcggcg
      301 gcacgaccga ggaaacgacc agtccgtcga ggtcgtcgaa gccggtaccc cgcagttcca
      361 tcaggccgga tatccgttcg gccagctcgt cgctggtcgc cccggccctc gtctgaaaac
      421 gccagtgacg ggtcagttcg ttgccctcga aggctccgag atgggtctgg gtgttgccga
      481 cgtcaattgc cagaagcatg aacaagggag tgtggcaaat gtcaagactt atgatcctcc
      541 ccatgagcaa ggcgacgctg ttctcacttc ccggttccca tcccgcggtc agcgtgaagc
      601 tgatgctcga ttacaagggc atcgacttca agacgatcga cctgatgccc ctcatgcaca
      661 aggccgtggt gcgtgccgcc ggcttccccg ggaccacggt tcccgccctc aagttcaacg
      721 gcgagaagct tcagggttcg gttgacatcg cgcgggcgct cgaccggatc gtgccggagc
      781 cgccgctgct gcccgccgac gaggacgccc gcaagcaggt gctgatggcc gaggccttcg
      841 gcgaggccga actccagcat gcggtccgcc agatcctctg gaatgcggtc cgtcggcagc
      901 cgaagtcgat cgccaccttc ctcgaaggtt cgagcctgcc cctgccgccc aaggtcgccg
      961 cctacacttc cggcccgatc atctggggcg agtacaaggt gaacgaatca accgacgaga
     1021 acgtgaaggc aaacctgatc gcgctgccgg agtggctcga ccgcctggac gcctgggtgg
     1081 ccgacggcac gctcggcggt gccaccccga acgccgccga tttccagatc gccaccagca
     1141 tccgcctggc ggccaccctt caggacctcc gccccttcct cgaaaaccgc ccgatctacg
     1201 aggccgcagt ccgcaccgct ccggtctacg cgggtgacgt ccccccgggc ctgccgaccg
     1261 agtggctcgc cccgctccgc gaaaacgccg ccgcccccgt ttccgactga gccccggccc
     1321 cggctcccgt ttccgactga cccccggcct cggcccgcgt tccgactgag ccccggcccc
     1381 ggctcccgat cgtcgagtgc ccactttgcg ccgcataccg gcacaaagtg gaacttcgcg
     1441 gccttccggc cctccgggtt tgcggtcctc tagatcgtgg cgaggtcggt gtcggggtcg
     1501 gcgagggcga cgaggtcgat ctgccgcttt gacttgatca gctcgccgat cgtctcgctg
     1561 acgtcccaga tattcacgtt catcccggcc tggatccggc cgtccttcag ccagaaggcg
     1621 atgaactcgc ggttttcctt gctgccgcga atcaccacct cgtcccagtc gctggcgaaa
     1681 ccgacgtact ccatgccgac gtcgtactga tcggagaaga aatagggcag gtcgtcgtag
     1741 atgacctcgg ccccggccat tgaccggccc gcggcgaagc cctgacgccg ggcgttggcc
     1801 cagtgctcga cccggatccg gtcgttgaag aagggatgga actggttggc gatgtcaccg
     1861 gccgcgtaga tcccctccgc ctcggtccgc aggccggcat cgaccggaac cccgttgttc
     1921 agctcgagcc cggccgcctc ggcgaggccg gtccgcggca ccgcaccgac gccgaccacg
     1981 acgaggtcgg cttcgatctc gccggccgtg gtccggactt tcgagaccga gccttcgccg
     2041 aggaaggcct cgaccccggt gccggacttg aagtcgacac cctgctcgag atggatctcc
     2101 gcgtacaccc cgccgacctc ggtccccagc actgactgga gcggaacctc ctgtggttcg
     2161 accagggtga cttcgcagcc cttctgccgg gccgaggcgg cgacttcgga gccgatccag
     2221 ccggcgccga ccacgaccag cctggttccc tcccggaacc gctcgccgag ttcggccgag
     2281 tcgcctgcac tgcgcaggta gtggattccg tcgagttcgg ctccggggat gtcaatccgg
     2341 cggggctcgc agcccgttgc cagcagcagc cggtcatacg gctccgggtc gcccccctcg
     2401 accagaactg ttcccgccga gggatcgatc ccggagacga aggtcgaggt ccgcaggtcg
     2461 atgtcgtgtt cggcatagaa cgactcctca tggacccagg cgaggtcgtc cttctcgcct
     2521 cgcagatagt ccttcgaaag cggcggccgt tcgtagggcc gctcgggctc gtccccgatc
     2581 agcacgatct cgccctcgaa tccggcctcc cgcgcacctt cggccgcccg cgccccggcc
     2641 aggctcgccc cgacgatcac gatcttctcg gcatcactca tctccgcctc cgttcaggtc
     2701 tgttcttcaa agctactccc ccgatctctc cggcccgtaa cctgaatcac tgccaccggc
     2761 agcagagacc ggatccagga cccagaggaa atgccattgc gatcagaaga gaggttcccc
     2821 atccgccgga gcgtcaccct gatcgtggcc gccttcaccc tgacgggatc ggcattcgcg
     2881 gtttcgggtt gcggcggcag cggagaagaa ccgaaacagt ggccgaacga cgcgggctat
     2941 ccgtttgacg aagtgaagct ggctgcagac ggtgtacccc gaaccgcgaa actcactgcg
     3001 gaccagaaag ccgagatcct gagaatcgcc aggaacgatc cgctcgtgaa ggaactactg
     3061 gaaggccatg aggtggagtg gcgcaagccc tacccctggt cccgtccgaa catggaactc
     3121 ctgggaggag gagtcgatgt ccattttgcc gagccggtcg acctgtctgg cgccacgatt
     3181 cccgccatcg agaacatcga ggagctgtac ggcaagcaag gcgaggaagg agtgatcctc
     3241 gaagaaggcg agcctcgcgt agagccggag atcggaacta cccggtcccc ttacacggac
     3301 gtgcgtgcct tgagcctgct ggtcgacctc gagcagggcc gggtcgccca ggtgctccag
     3361 attggctgag atgtccttct ctcagtcggt atcggaagcc gtcgatccgg acgtcgtctc
     3421 ctgcctgccc ggtgggggcg gggacatcaa tcgggggtgg cggatcgaga ccgctgaagg
     3481 agaccggttc tttgccaaga gccggcccgg cgggtcggtg gcggagtttg tgaacgaggc
     3541 ggccgggctt cgttggctcg gtgaggtcgg ggcgctgccg gtgcctgaag tgatcgcggt
     3601 tgtcgaagac gagaccgagc cggggttgat cctcgactgg gtcgagcccg ggccacgggc
     3661 tgacgaggcc gagcttggtc gcgggctggc gctcatccat caggcaggtg ccgatggatt
     3721 cgaccggttg gcaccggggg ccaccgggaa cgagatccgg ttcggtgagg ccgcgatgcc
     3781 gctgaatcca gtcccggccg gggaagggtt cgcgggggtc tatgcggccc gcctgcggca
     3841 ccttggcggc gaggcgtacg agcacggcgc gctcgggtcc tcggatcgcg atgtgatcga
     3901 gcaggtcgcc gaccgggccg acggtctggc cgggccggtc gaacctccgg cccgggtcca
     3961 cggcgacctc tggtccggca acctgctctg gagcgagggt cggccctggc tgatcgaccc
     4021 ggccgctcac ggcggccact gcgaaatcga cctggcgatg ctcgagcttt tcggtagtcc
     4081 cggacccgct ttctattcgg cctacgagga agtcgcgccg ttggccgacg gataccggga
     4141 acggatcggc ttctggcagc tccagccgct gctggttcat gcggtcctct tcggtggtgg
     4201 ttacggccga agcgctgcgg tcaccgcccg ggcctgtctc ggctgaggga atcgaagtgc
     4261 agttggtcgg cgatattcgc tgaccaactg catctcacgg accctttatg agaaacgaag
     4321 tgtttatggc taccccgatg gggtagtaga tctcgcatga acaaatggag atacctgctt
     4381 gtttctgtcc ttgcggcgct tgtggccggc ctgaccatcc cggcggcctc gtcggcctgg
     4441 gcaccggctg cttcggcgac cgtccacccc ggcgtccaga ccgtgaccga gggcgcccag
     4501 tgcacctcga acttcgtctt ccagaacggg tccgacgtct acctcggcca gg
//
LOCUS       DAOQFI010000049         4525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8126_length_4525_cov_3.386353, whole genome shotgun sequence.
ACCESSION   DAOQFI010000049 DAOQFI010000000
VERSION     DAOQFI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8126_length_4525_cov_3.386353"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLE93_01555"
     CDS             <1..949
                     /locus_tag="PLE93_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85763.1"
                     /translation="ITQTESEANNQRADVVLPAGLGVDGGRFDQTCPLADFQAGNCPE
                     STKVGEATAVSPLLDSDLSGPVYAVGADGLPQLGVDLKGTLPLKILGSSGFRNGRVET
                     IFEGLPDLPLSRFELVFPGGPTGLIRVIGDVCAESSTFNADFTSQAGQTVSRTLPTTE
                     TGTCPSGNGGGNGNGNGNGNGGNQVKKPKAKVKVSGAKNHRPRASIRVRSGGARMRVV
                     QVELPAALKLNKAAAKHLVAKADGKRVRGGKLVAKGRTLKLRGLPGKGAGVVTVNIPP
                     AAIAAPGRARKGSKLTFRLKVKRTGQKPVSLKASARVTK"
     gene            1007..1627
                     /locus_tag="PLE93_01560"
     CDS             1007..1627
                     /locus_tag="PLE93_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH85764.1"
                     /translation="MPATGQSGTGRQREQSEVSRQKLLDAAAIVFARQGFHKASLQAI
                     GEEAGISRGSIVWHFGTKDALLEAVVERSYLSWAREVESSEAGKPGGRQAIVRVIEAH
                     RRFIEESPAENRLYYALIAEVSANEELAERVRKMHAELYLQLHGYIKEGQEAGEISSQ
                     LDPEVGARLVLSTLGGIGHLVILVPDADVGPIYREVLRSMEANLAI"
     gene            1699..2913
                     /locus_tag="PLE93_01565"
     CDS             1699..2913
                     /locus_tag="PLE93_01565"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNH85765.1"
                     /translation="MSTDALIFDAIRTPRGKGKSSGSLYSTKPVDLVVGLMHETLIRN
                     EKLDPNTIDDVVLGCVSPVGDQGADIAKTAAIKAGLPDTVAGVQLNRFCASGLEAVNI
                     AAQKVASGWEDLVLAGGVESMSRVPMGSDGGAWAMDPETNYDTSFVPQGIGADLIATI
                     EGFTRDDVDEFAVRSQERAATAQKEGRFANSVIPVKDFNDMVVLDHDEFIREGTTTES
                     LAKLNPSFAGMGEMGGFDAVALQKYHWVEQIDPVHHAGNSSGIVDGASLVAIGSEKAG
                     ERNGMTPRARIVATAVSGADPTIMLTAPGPASKKALEKAGLTADDLDLIEINEAFAAV
                     ALRLVRDLDVDMEKVNVNGGAIAMGHPLGATGAMILGTIVDELERTGGRYGLCTLCVG
                     GGMGIATIVERV"
     gene            2942..>4525
                     /locus_tag="PLE93_01570"
     CDS             2942..>4525
                     /locus_tag="PLE93_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNH85766.1"
                     /translation="MSETKTIQWEKGDDGIVILTIDDPNQGANTMNRDYIESMGQIVD
                     RLEAEKESVTGVIITSGKKTFFAGGDLNDLLAVTKEQSVEFAAGIREVKSQLRRLETL
                     GVPVVAAVAGSALGGGLEITLATHHRIVVDDPKIQLGTPEVQLGLLPGGGGVVRTVRM
                     LGLADALMQVLIQGKPLRPAKAREIGLIDELVQSPDDLIPAAKKWIGENPEAVQRWDV
                     KGYKIPGGTPSSPKLAMTLPAFPSNLKKQLKGAKYPAPIAIMSAAVEGSQVDFDTAIE
                     IEGRYFLELATGQIAKNMIQAFFFNLQEVNGDRGRPADGERYTAKKVVVLGAGMMGAA
                     IAYVCAKAGIEVVLKDISLEAAERGKGYSKDIVDKAISRGRSTQEKGDALLALITPTA
                     EAKDADGADLVIEAVFEDPAVKAQVFAEIEPFLAEGALLGSNTSTLPITGLAEGVSRP
                     ADFIGLHFFSPVDKMPLLEIIKGEETSDTTLHRSLDLARQIRKTPIVVNDSRGFFTSR
                     VIGTFINEGIAFLAEGVPAS"
BASE COUNT          851 a         1489 c         1506 g          679 t
ORIGIN      
        1 gatcacccag accgaatccg aggcgaacaa ccagcgtgcc gacgtggtgc tgccggccgg
       61 gctcggagtc gacggtggcc gcttcgacca gacctgcccg ctcgctgact tccaggccgg
      121 aaactgcccc gaatccacca aggtcggtga ggcgacggcg gtcagcccgc tgctcgattc
      181 cgatctgtct ggccccgtat atgcggtagg tgccgacgga ctgccccagc tcggcgttga
      241 cctgaagggc acgctcccgc tgaagatcct cggctcttcc ggcttcagga acggccgcgt
      301 ggagacgatc ttcgagggtc tgccggacct gcccctctcg cgtttcgagc tggtcttccc
      361 cggcgggccg accggcctga tcagggtgat cggggatgtc tgcgccgaat cttcgacctt
      421 caatgccgac ttcacttccc aggccggcca gaccgtgagc cgaaccctgc cgacgaccga
      481 gaccggcacc tgcccgagcg gaaatggtgg aggcaacggc aacggcaatg gcaacggcaa
      541 cggcggcaat caggtcaaaa agcccaaggc gaaggtcaag gtaagcggag cgaagaacca
      601 ccggccgcgg gcgtcgatcc gggtcaggtc cggcggggcc cgcatgcgcg tcgtccaggt
      661 cgagctgccg gcggcgctca agctgaacaa ggccgcggca aagcacctcg tagccaaggc
      721 cgacggcaag cgtgtccggg gcgggaagct cgtggcaaag ggcaggaccc tcaaactgcg
      781 cgggctgccc ggcaagggag ccggtgtcgt gacggtgaac attccgccgg cggcgatcgc
      841 cgctcccggg cgtgcgcgca agggcagcaa gctgaccttc cggctgaagg tcaagcggac
      901 ggggcagaaa ccggtcagcc tcaaggcttc cgcccgggtc actaaatagc cggccgcggg
      961 tggcggcagg gccgcccgca gccggaggag tcgcgatact tgccgcgtgc cagccacagg
     1021 tcaatccgga accgggcgcc agcgggagca gtccgaggta tcccggcaga agcttctcga
     1081 cgcggccgcg atcgtgttcg cgcggcaggg ctttcacaag gcctcgcttc aggcgatcgg
     1141 agaagaggcc gggatcagcc ggggctcgat cgtctggcat ttcgggacca aggacgccct
     1201 gctcgaggcg gtggtcgagc gctcctatct ttcctgggcc cgggaggttg agtccagcga
     1261 agccggaaag ccgggcggtc gccaggcgat cgtcagggtg atcgaggcgc atcgccgttt
     1321 catcgaggag agcccggccg agaaccgtct ctactacgcc ctgatcgccg aggtgagcgc
     1381 caacgaggag ctggccgagc gggtgcggaa gatgcacgcc gagctctacc tgcagctcca
     1441 cggctacatc aaggaaggcc aggaggcggg agagatctcg agccagctcg acccggaagt
     1501 cggggcgagg ctggtgcttt caaccctggg cgggatcggc cacctggtga tcctcgtgcc
     1561 ggatgcggat gttggtccga tctatcggga agtgctccgc agcatggagg cgaatctggc
     1621 tatttgacat aattttgggg ccattcctgt gtcagccagc acagaaaaat cacttctctt
     1681 ccgaaacgga gcctcccgat gagcaccgac gcactgatct tcgacgccat ccgcacaccg
     1741 cgcggcaaag gcaagagcag cggctcgctt tactcgacca agccggtcga cctcgtggtc
     1801 ggactgatgc acgagaccct gatccgcaac gaaaagctcg atcccaacac catcgacgac
     1861 gtcgtgctcg gctgcgtttc accggtcggt gaccagggcg ccgacatcgc caagaccgcg
     1921 gcgatcaagg ccggcctgcc ggacacggtt gccggagtcc agctcaaccg cttctgcgcc
     1981 tccggcctcg aagcggtgaa catcgccgcc cagaaggtcg cctcgggctg ggaggacctg
     2041 gtcctcgccg gtggcgtcga atcgatgtcg cgcgtaccga tgggctccga tggcggtgcc
     2101 tgggcgatgg accccgagac caattacgac accagtttcg tcccgcaggg gatcggggcc
     2161 gacctgatcg cgacgatcga aggtttcacc cgggatgacg tcgacgagtt cgccgtccgc
     2221 tcacaggaac gggccgcgac agcccagaag gaaggccgtt tcgccaactc cgtgattccg
     2281 gtcaaggact tcaacgacat ggtcgtgctc gaccatgacg agttcatccg cgagggcacc
     2341 acgaccgagt cgctggccaa gctgaatccc tctttcgccg ggatgggcga gatgggcgga
     2401 ttcgacgccg tcgcgcttca gaaataccac tgggtcgagc agatcgaccc cgtccaccac
     2461 gccggcaact cttccggaat cgtcgacggc gcctcgctcg tcgcgatcgg cagcgagaag
     2521 gccggcgagc gaaacggcat gaccccgcgg gcccgcatcg tcgccaccgc ggtttccgga
     2581 gccgacccga cgatcatgct caccgcgccc ggcccggcca gcaagaaggc gctcgagaag
     2641 gcgggcctca ccgccgacga cctcgacctg atcgagatca acgaggcctt cgccgcggtg
     2701 gcgctgcgac tggtccgcga cctcgacgtt gacatggaga aggtcaacgt caacggcggc
     2761 gcgatcgcca tgggccaccc gctcggcgcg accggggcga tgatcctcgg cacgatcgtc
     2821 gacgagctcg agcgcaccgg cggccgttac gggctctgca ccctctgcgt cggcggcggc
     2881 atgggaatcg cgaccatcgt cgagcgcgtc tgatccaggg aaagaccagg agaacctgaa
     2941 catgagtgaa accaagacca tccagtggga aaagggcgac gacggaatcg tcatcctgac
     3001 catcgacgat cccaaccagg gcgcgaacac gatgaatcgc gactacatcg agtcgatggg
     3061 tcagatcgtc gaccgcctcg aggccgagaa ggaaagcgtc accggcgtga tcatcacctc
     3121 gggcaagaag accttcttcg ccggcggtga cctgaatgac ctgctcgccg tgaccaagga
     3181 gcagtcggtc gaattcgcgg caggcatccg cgaggtcaaa tcgcagctgc gccgcctcga
     3241 gacgctcggc gtcccggtcg tggccgcggt ggccggttcg gcgctcggtg gcgggctcga
     3301 gatcacgctc gccacccacc accggatcgt ggtcgacgac ccgaagatcc agctcggtac
     3361 cccggaagtc cagctcggcc tgctgcccgg cggcggcgga gtggtccgca ccgtacggat
     3421 gctcggtctg gccgatgccc tgatgcaggt gctgatccag ggcaagccgc tccgtccggc
     3481 caaggccagg gagatcgggc tgatcgacga gctggtccag tcgcccgatg acctgatccc
     3541 ggccgcgaag aagtggatcg gcgagaaccc ggaagcggtc cagcgctggg atgtgaaggg
     3601 ctacaagatc ccgggcggca ccccgtccag cccgaagctg gcgatgaccc tgccggcgtt
     3661 cccttccaac ctgaagaagc agctcaaggg tgcgaagtac ccggcgccga tcgcgatcat
     3721 gtcggccgcg gtcgagggtt cgcaggtcga cttcgacacc gcgatcgaga tcgagggccg
     3781 ttacttcctc gagctggcca ccggccagat cgccaagaac atgatccagg ccttcttctt
     3841 caaccttcag gaagtaaacg gtgaccgcgg ccgccccgcc gacggcgagc ggtacaccgc
     3901 gaagaaggtg gtggtgctgg gtgccggaat gatgggcgcc gcaatcgcct atgtctgcgc
     3961 caaggcgggc atcgaggtgg tgctaaagga catctcgctg gaggccgccg agcggggcaa
     4021 gggttactcg aaggacatcg tcgacaaggc gatctcccgc ggccgttcga cccaggagaa
     4081 gggcgatgcc ctgctggccc tgatcacccc gaccgccgaa gcgaaggacg ccgacggcgc
     4141 cgacctggtg atcgaggcgg tcttcgagga cccggcggtc aaggcccagg tcttcgccga
     4201 gatcgagccc ttcctggccg aaggcgcact gcttggctcg aacacctcga ccctgccgat
     4261 caccggtctg gccgagggcg tttcacgccc ggccgacttc atcggccttc acttcttcag
     4321 cccggtcgac aagatgcccc tgctggagat catcaagggc gaagagacca gcgatacgac
     4381 gctccaccgc tcgctcgacc tcgcccggca gatcaggaag acgccgatcg tggtcaacga
     4441 ttcacgcggc tttttcacca gccgggtgat cggcaccttc atcaacgagg gcatcgcctt
     4501 cctggccgag ggagtgccgg cttcg
//
LOCUS       DAOQFI010000050         4522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8139_length_4522_cov_3.813969, whole genome shotgun sequence.
ACCESSION   DAOQFI010000050 DAOQFI010000000
VERSION     DAOQFI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8139_length_4522_cov_3.813969"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2963)
                     /locus_tag="PLE93_01575"
     CDS             complement(<1..2963)
                     /locus_tag="PLE93_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNH85767.1"
                     /translation="MAERDYLSAISDHIVVFDGGMGATLEMFDLTQEDYGGLAGKCHE
                     ALVLNRPDVIEGVHTSMIEAGAEVVETDTFQASRIKLDEWGLADHTEEINVKAAEIAR
                     KAAGPDRFVAGSIGPTGHLPASDDPTLGQIRFPELVTVFTEQAGGLVKGGADLIIIET
                     AQDILEVKAAIFGARAAFEEIGRSVPIQVSVSLLPNGGKMLLGTDIQAVLTTLEALKV
                     DVIGLNCSTGPEDMRDAIRYLGENSAVPVHCIPNAGLPLQGPDGETIFPEKPEPLAAT
                     LGEFVERYGVGVVGGCCGTTPEHIRAISERVKDLKPGPRPEPGEIRVSSMMTSTALVQ
                     EPTPTMVGERVNSQGSRKAKELLLADDYDGLLQVAEDQVEGGAHVLDVCVALTERQDE
                     DEQMRQVVKRISLTQPSPIQVDSTEPDVIRTALEQIPGRAIVNSVNLEAGRDKLDTVA
                     PLAKAHGACLIALTIDEVGMAKTADRKVEIARRITEMVCDEHGLDREALIFDDLTFTL
                     TTGDEEWRPSAIETIEGIRRIKAEIPGVKTSLGVSNVSFGVGQPARAVLNSVFLHHCV
                     EAGLDLAMVNPNHIKPYGEIPAEEIELTEDLVFNRREDALERFIAHFESKGDEAEEEA
                     EDATAGMEPEEALHFMILRRKKEGVEDWIDKSVEKIGAVPTLNDVLLPAMKEVGDKFG
                     AGELILPFVLQSAEVMKKAVSQLENYLDRIEGHTKGKVVIATVFGDVHDIGKSLVNTI
                     LTNNGYTVIDLGKQVPVDTIIDAAIEHEADAIGLSALLVSTSKQMPIAVQELHQRGLD
                     YPVLIGGAAINRDFGRRTLYPHGKESDEVYEPGVIYCKDAFQGLDTMDALIDDEARAA
                     LIDRLKQEAEEYRNKPVIVEDLPPLDDDSVRSSASTDAPIPSPPWWGVREVPVDFDEV
                     FDHLDLHVLFKLHWGGKGVKGEEWDKLVCEEFRPRLERMWREQDYLHPKARLGYFPCN
                     ADGNELVIFDPEDQD"
     gene            3033..3551
                     /locus_tag="PLE93_01580"
     CDS             3033..3551
                     /locus_tag="PLE93_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNH85768.1"
                     /translation="MVGAISYVALLRAVNVGGTGKLPMADLRKLAGQLGFGDVRTYIA
                     SGNLLITSGLTEAQVKKQLEAALRDYAGREIGVIVRTAAEMRAVELANPFKSAAYNRI
                     GVLFLDHRLPRENIRKAKGRKNEEIEIGKREIYVHYPDGMGRSKLNLPAAKYGTTRNL
                     NTVSKLADMAGG"
     gene            complement(3552..4334)
                     /locus_tag="PLE93_01585"
     CDS             complement(3552..4334)
                     /locus_tag="PLE93_01585"
                     /inference="COORDINATES: protein motif:HMM:NF018031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNH85769.1"
                     /translation="MAGPHAINGFDRTLSRIGTASADGYGIIVRRLKGPGDGWFSASE
                     LDGSGRVQALLLSRISGMAGPPEDHLRAEWLLESLARAIADLGGSFIVAAGQLPELTP
                     ENILLAAEGGLFRATAVASDRGLETTDPQSLATAAGPSDLDVLAEAFRRGFADLLAPT
                     VDWLDAQGLRPAKTLWHAAADRLAQALVWSGKAFDRPGLALELTRLTVGPDCPDPRLA
                     IPIERAEDDWGDEYHLRSTCCLAYRTEGGQLCQSCPLVKDRD"
     gene            complement(4339..>4522)
                     /locus_tag="PLE93_01590"
     CDS             complement(4339..>4522)
                     /locus_tag="PLE93_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85770.1"
                     /translation="LSEYFKVTAVRDGQAKWAEFQFGNLKKESKLEPTKEENGTSIHF
                     KPDNGPAGNLTIRVKQ"
BASE COUNT          708 a         1455 c         1537 g          822 t
ORIGIN      
        1 ctgtcctggt cctccgggtc gaagatgacc agctcgtttc cgtccgcgtt gcaggggaag
       61 taaccgagcc gggccttcgg gtgaaggtag tcctgttccc gccacatccg ctccagcctc
      121 ggccggaact cctcgcatac gagcttgtcc cactcctcgc ccttgacgcc cttgccgccc
      181 cagtggagct tgaagagcac atgcaggtcg aggtggtcga agacctcgtc gaaatcgacc
      241 ggtacctccc gcacgcccca ccagggcggc gacgggatcg gcgcgtcggt gcttgccgaa
      301 gagcggaccg aatcatcgtc cagcggcggc aggtcctcga cgatgaccgg cttgttgcgg
      361 tactcctcgg cctcctgctt gaggcgatcg atcagcgccg cgcgggcctc gtcgtcgatc
      421 agggcgtcca tcgtgtcgag gccctggaag gcgtccttgc agtagatgac gcccggctcg
      481 tagacctcat cggactcctt gccgtgcggg tagagcgtgc ggcggccgaa gtcgcggtta
      541 atcgcggccc cgccaatcag caccgggtag tcgagcccgc gctggtggag ttcctggacg
      601 gcgatcggca tctgcttcga ggtcgagacg agcagggcgg agaggccgat cgcgtcggcc
      661 tcgtgctcga tcgcggcatc gatgatcgtg tccaccggaa cctgtttgcc gaggtcgatc
      721 accgtgtagc cgttgttggt caggatcgtg ttgaccagcg acttgccgat gtcatgaacg
      781 tcaccgaaga cggtcgcgat cacgaccttg cccttggtgt ggccttcgat ccggtcgagg
      841 taattctcga gctgcgaaac agccttcttc atcacctcgg ccgactgcag cacgaagggc
      901 aggatcagct cgccggcccc gaacttgtcg ccgacctcct tcatcgccgg caacagcacg
      961 tcgttcaggg tcgggaccgc gccgatcttc tcgaccgact tgtcgatcca gtcttcgacg
     1021 ccttccttct tgcggcgcag gatcatgaag tggagcgctt cttccggctc catccccgcg
     1081 gtcgcgtcct cggcttcctc ctcggcttcg tcgcccttcg actcgaagtg ggcgatgaac
     1141 cgttccagcg cgtcctcgcg ccggttgaag accaggtcct cggtcagctc gatctcctcg
     1201 gccgggatct cgccgtacgg cttgatgtga ttcgggttga ccatcgcgag gtcgaggccg
     1261 gcctcgacgc agtgatggag gaagaccgag ttgagcaccg cgcgggcggg ctggccgacg
     1321 ccgaagctca cgttcgagac gccgagcgag gtcttgactc cggggatctc cgccttgatc
     1381 cggcggatgc cctcgatcgt ctcgatcgcg gacgggcgcc actcctcgtc acccgtggtc
     1441 agggtgaagg tgaggtcgtc gaagatcagg gcttcccggt cgaggccgtg ctcatcgcaa
     1501 accatctcgg tgatccgtcg ggcgatctcg accttgcgat cggcggtctt ggccatgccg
     1561 acctcgtcga tggtcagggc gatcaggcag gcgccgtggg ccttggccag cggggccacg
     1621 gtgtcgagct tgtcgcggcc ggcttcgagg ttcacggagt tcacgatcgc ccggcccggg
     1681 atctgctcca gggcggtcct gatcacgtcc ggctcggtcg agtcgacctg gatcggtgaa
     1741 ggctgggtca gcgagatccg cttgacgacc tgccgcatct gctcgtcctc gtcctggcgt
     1801 tcggtcagcg cgacgcagac gtcgagcacg tgggctccgc cttcgacctg gtcctcggcg
     1861 acctggagca gtccgtcgta atcgtcggcc aggagcagct ctttcgcctt gcgggagccc
     1921 tgggagttga cccgctcccc gaccatggtc ggggtcggtt cctggaccag cgcggtggaa
     1981 gtcatcatcg aggaaaccct gatctcgccc ggctcgggcc gcgggcccgg cttcaggtcc
     2041 ttgacccgct cgctgatcgc ccggatgtgc tccggcgtgg tgccgcagca gccgccgacc
     2101 acgccgactc cgtagcgctc gacgaactcg cccagcgtcg cggccagcgg ttccggcttt
     2161 tcggggaaga tcgtctcgcc gtccgggccc tgcaggggca ggccggcgtt ggggatgcag
     2221 tgaaccggaa ccgccgagtt ctcgccgagg tagcggatcg catcccgcat gtcctcggga
     2281 ccggtcgagc agttgaggcc gatcacgtcg accttgaggg cttcgagcgt ggtcagcacc
     2341 gcctggatgt cggtgccgag cagcatcttg ccgccgttgg gcagaagcga gacggaaacc
     2401 tggatcggga cgctgcggcc gatctcctcg aaggcggcgc gggctccgaa gatcgccgcc
     2461 ttgacctcga ggatgtcctg ggcggtttcg atgatgatca ggtcggcgcc gcccttgacc
     2521 aggccgccgg cctgttcggt gaagacggtg accagctcgg ggaagcggat ctgcccgagg
     2581 gtcgggtcat ccgaggcggg caggtgcccg gtcggtccga tcgagccggc aacgaaacgg
     2641 tccgggccgg ctgccttgcg ggcgatctcg gccgccttga cgttgatttc ctcggtgtgg
     2701 tcggcgagac cccactcgtc tagcttgatc cgggaagcct ggaaggtgtc ggtctcgacc
     2761 acctcggcgc cggcttcgat catcgaggtg tgaacccctt cgatcacgtc gggccggttc
     2821 aggaccaggg cctcgtgaca tttgccggcc aggccgccgt aatcctcctg ggtcaggtcg
     2881 aacatctcca gggtcgcgcc catcccgccg tcgaagacga cgatgtggtc ggaaattgcg
     2941 ctcagatagt cacgttcggc catcaagcga ggataccgat cagctaaatc cgatcaggat
     3001 tcgggatttc gcgttgccct agtcttagga ccatggtggg ggccatttca tacgtcgcgc
     3061 tgctcagggc cgtgaacgtc ggcggcaccg gcaagctgcc gatggccgat ctgcggaaac
     3121 tggccggaca gctcggtttc ggggatgtga ggacctacat cgccagcggc aatctgctca
     3181 tcacttccgg gctgaccgaa gcgcaggtga aaaagcagct cgaggcggcg ttgcgtgact
     3241 acgcggggag ggaaatcggc gtgatcgtcc ggaccgcggc ggagatgcgg gcggtcgagc
     3301 tggccaatcc gttcaagagc gccgcctaca accggatcgg cgtcctgttt ctcgatcacc
     3361 gcctgccgcg ggagaacatc aggaaggcga agggccggaa gaacgaggag atcgaaatcg
     3421 gcaaacggga gatctacgtc cactacccgg acgggatggg caggtcgaaa ctgaacctcc
     3481 ccgccgccaa gtacggcaca acgagaaacc tgaacacggt cagcaagctg gccgacatgg
     3541 ccggcggctg atcagtcccg gtccttgacc agggggcagg actggcagag ctggccgcct
     3601 tcggtgcggt aggccaggca gcaggtgctc cggaggtggt actcgtcgcc ccagtcgtcc
     3661 tcggctcttt cgatcgggat cgcgagccgg gggtccgggc aatcgggacc gacggtcagc
     3721 ctggtcagtt cgagcgcaag tccgggacgg tcgaaagcct tgccggacca gaccagtgcc
     3781 tgggccagcc ggtcggcggc cgcgtgccag agcgtcttgg ccggtctgag gccctgggcg
     3841 tcaagccagt caacggtcgg ggccagcagg tcggcgaaac cgcggcggaa cgcttcggcg
     3901 aggacatcga ggtccgacgg tccggccgct gtggcgagcg attgcgggtc ggtagtttcg
     3961 aggccccggt cgctggccac cgccgtcgcc cggaagaggc cgccctcggc tgccaacagg
     4021 atgttctccg gggtcagctc cggcaactgg ccggcagcga cgatgaacga gccgccgagg
     4081 tcggcgatcg cccgggccag gctctcgagc agccactccg cccgcaggtg atcctcgggc
     4141 gggccggcca tgccgctgat ccgggaaagc aggagcgcct ggacgcggcc gcttccatcc
     4201 agctccgagg cggagaacca gccgtcaccc gggcccttca ggcgacggac gatgatcccg
     4261 taaccgtccg cggacgcggt gccgatccgg ctcagggtcc ggtcgaaccc gttgatcgca
     4321 tgcggcccgg ccatgcggtc actgtttcac acggatggtg agattcccgg cagggccgtt
     4381 gtcgggtttg aagtggatgc tggtgccgtt ttcctctttg gtcggttcca gtttgctttc
     4441 ctttttcagg ttgccgaact ggaattcggc ccatttggcc tgcccgtcgc gcacggcggt
     4501 cactttgaaa tattcggaca ag
//
LOCUS       DAOQFI010000051         4501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8191_length_4501_cov_3.307692, whole genome shotgun sequence.
ACCESSION   DAOQFI010000051 DAOQFI010000000
VERSION     DAOQFI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8191_length_4501_cov_3.307692"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLE93_01595"
     CDS             complement(<1..406)
                     /locus_tag="PLE93_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH85771.1"
                     /translation="MDSESQALPTLRQQGDVYLLDLGDGENRFNPDRNSVIEELLAEV
                     EAAPAPRALVTVASGKVWSNGLDLEWFQAHPDRVQEALDQTERLFAAFLGAGIPTVAA
                     IQGHCFAGGAMLALAHDYLIMREDRGYLCLPEA"
     gene            486..1166
                     /locus_tag="PLE93_01600"
     CDS             486..1166
                     /locus_tag="PLE93_01600"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HNH85772.1"
                     /translation="MGEAVGQTLPLAVGVAISPIGIIASVVMLTSGRGAGNGLAFLVG
                     WFIGLAVACWVLLQLNLGADPASGGHPADWTSYAKIILGLLLLVVAHRQRGKKTAPGE
                     TPELPKWMDKADSIKPLRATGLAFLLGAVNPKNLILTMAAATAIGQVDAGSADQLTSV
                     LVYALIASVGAAVPVGIYFLMGDRADSMLARLKESMARNNGVIMAVICVVIAAKLIGD
                     GITTLSLF"
     gene            complement(1186..1869)
                     /locus_tag="PLE93_01605"
     CDS             complement(1186..1869)
                     /locus_tag="PLE93_01605"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNH85773.1"
                     /translation="MFDSDFDPRDRGRFEGRCGDPGFGPAAGDGQPGFGPETDSGRRR
                     YRHGPGRGGPHGFGGGHHGRGPGRPGRGRRARRGDIRQAILLLLEEEPRNGYGLMQEV
                     EERSGGAWRPSPGSVYPALSQLEDEGLVEMAESEGRKDFSLTGAGREWVEEHREEIGS
                     PWDTEGRGTPRELGSLRKSTQALAMAARQLAHTGTPAQLEEARKLVDEARRKLYGILA
                     QSEEEQEQS"
     gene            complement(1974..3782)
                     /locus_tag="PLE93_01610"
     CDS             complement(1974..3782)
                     /locus_tag="PLE93_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85774.1"
                     /translation="MMIRKQGENLCDTKVEQYQVMTVDALFGNPAVKLMRNNGKQDSS
                     LITRAPTLRDIRNRGEEAYIDLPGDPLGETCVYAKDFDRMRREGRAPVAVYAHIAREK
                     GHSGLALQYWFYWYFNQFNDLHESDWEGMQITFDANTPAGALKQEPHEMIVFQHAGGE
                     RANWDDAKVEKEGSHPVVYPAAGSHATFYQSAIYPQNGSHGSGVGCDNTSEPLTELRP
                     VPVLLPERPTDRGEFAWLSFDGRWGQKEKSFNNGPTGPQTKTQWDEPFTWMEAQRWSS
                     PRMPGGGLVGPEAVNAFCGVIESVTGVMNLQQSDPLAAYLIAAAFILIVFFVFGYSKW
                     RPAGADRLEQKRAYGQIMNASFRLYWRHLSAFAVLGAIAIPIVGGTQALGGWLGDMSG
                     GDGLLQTLSDLVNSFGRPAAQALISAFLIVFIRDLVQGQTATVRTALDGTRERFWRVV
                     IAKMLAIAGVTLLALTVIGIPFAVRFLVNWTFVQQEVIFTDLSIRDSFRASSDLVRGH
                     WWRTVRTMVPLLLLLTVLGPLLGLFLIFTPLPLLLVNLIGSVVFAIAIPFTTAGTTLL
                     YFDLRARQQEHGVVPRRSWAVWRPATFGRPVGQPTA"
     gene            4014..>4501
                     /locus_tag="PLE93_01615"
     CDS             4014..>4501
                     /locus_tag="PLE93_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH85775.1"
                     /translation="MRSDVPETVDLFHFDQPGAIAAHLFDGVIVDPGAEKTVDRLLDA
                     LGDEVPQAILLTHIHFDHAGATGRLVEKFPEVEVWVHESGAPHMIDPERLVSSARKVF
                     GQHFDAMWGEVVPVPDANIRTLKGGESDGPWRVQYTPGHAKHHVCYFHEPSRTAFTGD
                     VTG"
BASE COUNT          759 a         1507 c         1453 g          782 t
ORIGIN      
        1 cggcttcggg caggcataga tacccccggt cttcccgcat gatcaggtag tcgtgggcga
       61 gagcaagcat cgccccgccc gcgaagcagt ggccctggat cgctgccacg gtcgggatcc
      121 cggcaccgag gaaggcggcg aacagtctct cggtctgatc gagggcttcc tggacccgat
      181 ccgggtgcgc ctggaaccac tcgagatcga ggccgttgga ccagaccttt ccactcgcca
      241 cggtgaccag cgcccgcggc gcaggtgccg cttcgacctc cgcgagcagc tcctcgatca
      301 cggagttccg atccgggttg aaccgattct ctccgtcgcc gagatcgagc agataaacgt
      361 cgccctgttg cctcagggtc ggcaaagcct gactctctga atccatcgcg ctcccttctt
      421 gctggtttcc gccgagccta caggcgaaag ccgcgagctc cgatcgggcg ggttatcctg
      481 tggccgtggg agaggcggtt ggacagacac ttcccctggc cgtcggcgtg gcgatcagcc
      541 cgatcggcat catcgcctcg gtggtgatgc tgacttccgg ccgcggcgcc ggcaacggtc
      601 tcgcattcct ggtcggctgg tttatcggcc tggcggtcgc ctgctgggtc ctgctccagc
      661 tcaacctggg ggccgaccct gcctcggggg gccatcccgc cgactggacc agctacgcga
      721 agatcattct cggcctgttg ctgctggtcg tcgcccatcg ccagagaggg aagaaaacgg
      781 ctccaggcga aaccccggag ctgccgaagt ggatggacaa ggccgattcg atcaagccgc
      841 ttcgtgcgac cggcctcgcc tttctgctcg gcgcggtcaa tcccaagaac ctgatcctga
      901 ccatggccgc cgctaccgcg atcgggcagg tggacgccgg tagtgccgac cagctgactt
      961 cggtactggt ctacgccttg atcgccagcg tcggggcggc cgtcccggtc gggatctact
     1021 tcctgatggg tgaccgggcc gattcgatgc tggccaggct caaggagagc atggcccgca
     1081 acaacggcgt gatcatggcc gtgatctgcg tggtgatcgc cgccaaactg atcggcgacg
     1141 gaatcaccac gctttcactc ttctagatcg ccggcccgcg aaagctcagg actgctcctg
     1201 ctcctcttcg ctctgggcga ggatcccgta gagctttcgc cgggcttcat cgacgagctt
     1261 tcgcgcttcc tcgagctggg ccggtgtgcc ggtatgggcc agttgcctgg cggccatcgc
     1321 cagcgcctgg gtcgacttcc tcagggaacc gagttcccgg ggagtcccgc ggccttcggt
     1381 atcccaggga gaaccgatct cctcgcgatg ctcctcgacc cattctctgc cggcgccggt
     1441 cagggagaag tccttgcggc cttcggattc ggccatctcg accagacctt cgtcctcgag
     1501 ctgggagagc gccgggtaga ccgagccggg gctcggacgc caggcaccgc cgcttcgctc
     1561 ttcgacctcc tgcatcaggc cgtagccatt gcggggctcc tcttcgagca acagcaggat
     1621 cgcctgccgg atgtcacctc gccgggccct cctgccgcgt ccggggcgac caggcccccg
     1681 gccatggtgg ccgccgccga aaccgtgggg tccgcccctt cccggcccgt gccggtagcg
     1741 gcgccggccc gaatccgtct ccgggccgaa acccggctgg ccgtcaccgg ccgcggggcc
     1801 gaagccggga tcgccgcagc gaccctcgaa tcgcccgcgg tcgcgcgggt cgaaatctga
     1861 atcaaacatc tgaaaaacct ttcgtttcga atatctcacg atacataacg atatatcgtg
     1921 atactcaaaa tgtcaagagg cggtaccgcc ggttggatgc cccggcgcag gcttcaggcg
     1981 gtgggctgcc cgaccggccg gccgaaagtc gccggtcgcc agacggccca ggagcggcgc
     2041 ggcaccaccc cgtgttcctg ctggcgggct cgcagatcga agtagagcag ggtcgtgccg
     2101 gcggtcgtga acggaatcgc gatcgcaaag accaccgatc cgatcaggtt gaccagaagc
     2161 agcggcaagg gagtgaagat cagaaagaga ccaagcagcg gtcccagcac ggtcagcagc
     2221 aagagcagcg ggaccatggt ccggaccgtt cgccaccagt gcccgcggac cagatcgctg
     2281 ctggcccgga aggagtcacg gatcgacagg tcggtgaaga tcacctcctg ctggacgaag
     2341 gtccagttga cgaggaagcg gaccgcgaag ggaattccga tcacggtcag agcaagcagg
     2401 gtcactccgg caatcgcgag catctttgcg atcacgacgc gccagaaccg ttcccgggtg
     2461 ccgtcgagcg ccgtcctcac ggtcgcggtc tgcccctgaa ccaggtcacg gatgaacacg
     2521 atcagaaagg ccgagatcag cgcctgggcc gccggccggc cgaaactgtt gaccaggtcg
     2581 gagagcgtct gcagcaaccc gtccccgccg gacatgtccc ccagccagcc gccgagggcc
     2641 tgggtgccgc caacgatcgg gatcgcgatc gcccccagca ccgcaaaggc cgacaggtgg
     2701 cgccagtaca gcctgaagct cgcgttcatg atctgtccgt aggcgcgctt ttgctcaagg
     2761 cgatcggcac ccgcgggacg ccatttcgag tacccgaaca cgaagaagac gatcaggatg
     2821 aaagccgcgg ctatcaggta ggccgcgagc ggatcggact gctgcagatt catcacgccg
     2881 gtcacgctct cgatcacccc gcagaaggcg ttcaccgctt ccggaccgac cagccctccg
     2941 ccgggcatcc gggggctcga ccagcgctgc gcttccatcc aggtgaaggg ctcatcccac
     3001 tgggtcttgg tctgcgggcc ggtcgggccg ttgttgaagc tcttctcctt ctggccccag
     3061 cgcccgtcaa aggaaagcca cgcgaattca ccgcggtcgg tgggccgctc cggcaagagc
     3121 accgggaccg gcctgagctc ggtcagcggc tccgaggtgt tgtcgcagcc cactcccgat
     3181 ccatgcgagc cgttctgggg atagatcgcc gactggtaga aggtcgcatg ggaaccggcc
     3241 gccgggtaga cgaccgggtg gctgccttcc ttttcgacct tggcgtcatc ccagttcgcc
     3301 cgctctcccc cggcgtgctg gaagacgatc atctcgtgag gctcctgctt cagcgccccg
     3361 gccggtgtgt tcgcatcaaa ggtgatctgc atgccctccc agtcgctttc atgcagatcg
     3421 ttgaactggt tgaagtacca gtagaaccag tactggagtg ccaggccgga atgtcccttt
     3481 tcgcgggcga tgtgggcgta gaccgcgacc ggtgcccggc cttcacgccg catccggtcg
     3541 aagtccttcg catacacgca ggtctctccc agcgggtcgc cgggcaggtc gatgtaggcc
     3601 tcctcgcccc ggttcctgat gtctctcagg gtcggtgccc tggtgatcag gctggaatcc
     3661 tgcttgccgt tgttccgcat cagcttcaca gcggggttgc cgaacaacgc gtcgaccgtc
     3721 atcacctggt actgctcgac cttggtgtcg cagaggttct cgccctgctt gcggatcatc
     3781 atcaacgggg cagcgtgagt gcgtggcggt ctcgacttcg cgggccagct cgcgggccgc
     3841 gaccgaccgg gctccggcag cgtcggccgg agcgatcccg gcagatgaaa gcgtcaggag
     3901 ggcgagggcc aggggcagcg aaacgaatgc ggcaagcctc acccccagct tcgcttttcc
     3961 tccggacgcc acggggaaag attattcgtt cggcgtccgg taatttcggc gccatgcgaa
     4021 gcgacgtccc ggaaaccgtt gacctgttcc atttcgacca gccgggggcg atcgcggccc
     4081 acctcttcga cggagtgatc gttgatccgg gtgcggaaaa gaccgtggac cggttgctgg
     4141 acgctcttgg cgacgaggtt ccgcaggcga tcctgctcac gcacattcat ttcgaccacg
     4201 ccggcgccac cggccggctg gtcgagaagt tccccgaggt ggaggtctgg gtgcacgagt
     4261 cgggagcccc gcacatgatc gatcccgagc ggctggtgag cagtgcccgc aaggtctttg
     4321 gccagcactt cgacgcgatg tggggcgagg tcgtgccggt ccccgatgcg aacatccgga
     4381 cgctcaaggg cggcgaatca gacgggccct ggcgggtcca gtacacgccg ggtcacgcca
     4441 agcaccacgt ctgctatttc cacgagccgt ctcgcacggc ctttaccggc gacgtgaccg
     4501 g
//
LOCUS       DAOQFI010000052         4460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8330_length_4460_cov_3.434960, whole genome shotgun sequence.
ACCESSION   DAOQFI010000052 DAOQFI010000000
VERSION     DAOQFI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8330_length_4460_cov_3.434960"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /gene="ruvB"
                     /locus_tag="PLE93_01620"
     CDS             <1..687
                     /gene="ruvB"
                     /locus_tag="PLE93_01620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNH85776.1"
                     /translation="DEIHRLGRAVEETLYPAMEDGELPVVLGQGAGAQTVTLPLPPFT
                     LIGATTRSGLLTTPLRDRFGVSHRLEYYTPADLARIVIRSAGILGVEIDEGGAVAIAE
                     RSRGTPRVANRLLKRVRDFAEVKGSGSVDAEMADAALEMLEVDKAGLDRADRALLELI
                     ATRFQGGPVGLSTIAVAIGEEQDTIEDVLEPYLLQMGMLKRTPRGRVLTQSAFEHLGL
                     PVPSGHQSLF"
     gene            complement(684..1595)
                     /locus_tag="PLE93_01625"
     CDS             complement(684..1595)
                     /locus_tag="PLE93_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH85777.1"
                     /translation="MKIFIAGASGAIGLPLVRQLAAAGHEVVGSTRGGQGAARIREAG
                     GIAVACDVFDRAQLIEAVVKAEPEVVVNQLTSLPGKFEPKKKGFYDANNRIRSEGGDN
                     LIEATAAAGAPRVVTQSISFLYELSGPMIKAEEEPVDRSGVHDAVLGHERKSLEDDRF
                     EAVVLRYGLLYGPGTWYARDGYLSGEVRKRRLPIVGGGHGMTSFLHVEDAASAAVTAL
                     AKGEGIYNVTDDEPAATRDWIPVFAESVGAKPPRKVPFWLAALIAGKPIALQATEGRA
                     ASNAKFKAQTGWEPSIPSWRQGFFEAT"
     gene            1635..2123
                     /locus_tag="PLE93_01630"
     CDS             1635..2123
                     /locus_tag="PLE93_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH85778.1"
                     /translation="MPFFICPSCGNRENSGDRTAGFSPRPKGCSKCGFGFVFELLDDY
                     YPAPNAAFFVCDQEGRVIDAGRGSFQLTGLTDEDVIGRPVREVLGLEWIDPGDGGPEA
                     DTNGDGETDPIETSLEWGVRSLGKRVEVRSEKDSAAQAIADVFPAYDDDGGLMIVLTP
                     EG"
     gene            2136..>4460
                     /gene="secD"
                     /locus_tag="PLE93_01635"
     CDS             2136..>4460
                     /gene="secD"
                     /locus_tag="PLE93_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNH85779.1"
                     /translation="MGGRRRNIFVLLFVLGLVIVSGIVCLKKPTQLGLDLQGGIELTL
                     QGRPTPQVKEVTSEAMDRSVDIIRSGCDKLGVSEIEVSRLGADQISVGIPGATNVGTA
                     TECATKPARLYFYDWENNLVGPARDLSLVGTGADTAEVSKQVRKAWVDAGRPPTEGFN
                     RTWIGLGAEPTMWDAVNLAAKQPRDDKCEDCTSGDKYYLFDEKTHELISGPVTAKEDL
                     YYNDQGRPIPHKGIVKKVPQGTIIVDELPTDSDGKTIDDESQAGYFVLKDNAALSGSE
                     IKDPQQNLDQFNQPNVTFDFTGDGKDSFQEITRRIAQEGQARAIGGVTSADQASALSG
                     HFAIILDGQVVSRPIINFVENPDGIDGRQGAQISGGFTVDQAQNLAELLQRGALPIDL
                     KLTSQSQVSATLGQEALDQGVLALLVGLFCVIIFLIVFYRFLGGVASLALLVYAVLFL
                     ALIKVIPITMTLPGIAGLVLTIGVAADSNIVIFERVKEELRAGRSVQSAITLGYRRGI
                     STIIDANVVTLLTAFILFVLATSGIKGFAFTLGVGTIVSLLTAVVFTQALLGSMSNTK
                     LMRSDKAFAGMEGKERWHFDFMGSAPKFFAISGVILLIGAGALTTKGLNFGIDFESGT
                     RIEVALNDSASTEDVRSVLSPLGLGSADIQTITDNPTLGDNAFQIESETLDPSEVSKV
                     DDALNKEFGVSADGFNSTSVGPTFGASVARSAALAIFFSMILIMGYVAIRFGGKYTVP
                     VLIAIAHDFLIALGIYALLGFEVSSATVAAFLTIL"
BASE COUNT          757 a         1486 c         1418 g          799 t
ORIGIN      
        1 gatgagattc accggcttgg gcgggccgtg gaagagactc tctacccggc gatggaagac
       61 ggtgagctgc cggtggtgct cggccagggg gccggcgccc agaccgtgac cctgccgttg
      121 ccgccgttta ccctgatcgg ggccactacc cgcagcggcc tgctgacgac tccgctgcgg
      181 gaccggtttg gcgtctccca taggctcgag tactacacac cggccgatct ggccaggatc
      241 gtgattcgtt cggccgggat cctcggggtc gagatcgacg aggggggagc ggtggcgatc
      301 gccgagcgtt cccgcggcac gccacgggtc gccaaccgcc tgctcaaacg ggtgcgggac
      361 ttcgccgagg tcaagggatc cggttcggtc gatgcagaaa tggccgacgc tgcgctcgag
      421 atgctcgaag tcgacaaggc cggcctcgac cgggccgacc gcgccctgct cgagttgatc
      481 gcgacgcggt ttcagggcgg gccggtcggc ctttcgacga ttgcggtggc gatcggtgag
      541 gagcaggaca cgatcgaaga cgtgctggag ccctatctgc tccagatggg catgctgaag
      601 cgcaccccgc gggggcgggt tcttacgcag tcagctttcg agcacctcgg cctgccggtt
      661 ccctcgggcc atcagtcgct cttctaggtc gcttcgaaga atccctggcg ccaactgggg
      721 atcgacggtt cccatccggt ctgcgccttg aacttcgcgt tcgatgcggc ccggccttcg
      781 gtggcctgca acgcgatcgg tttgccggcg atcagcgcgg ccagccagaa cggaaccttg
      841 cgtggcggtt tggccccgac cgactctgcg aaaacgggga tccagtccct ggtcgcggcc
      901 ggttcgtcat cggtcacgtt gtagatcccc tcgcctttcg ccagggcagt gaccgcggct
      961 gaagccgcat cttcgacgtg gaggaaagag gtcatgccgt ggccgccacc gacgatcggc
     1021 agccgccgct tgcggacttc accggacagg tagccgtcac gggcgtacca ggtgcccggg
     1081 ccgtagagca gcccgtaacg gagcacgacc gcctcgaacc ggtcgtcttc gagcgacttt
     1141 cgctcgtggc caagcacggc gtcgtggacc ccgctgcgat caaccggttc ctcctcggcc
     1201 ttgatcatcg gtccgctcag ctcgtagagg aaggagatgc tctgggtgac cacgcggggg
     1261 gcgccggcgg cggccgtcgc ttcgatcagg ttgtctccgc cttccgacct gatccggttg
     1321 ttggcgtcat agaacccctt cttcttcggc tcaaacttcc cgggcaggct ggtcagctgg
     1381 ttgaccacga cttcgggttc ggccttgacg acggcctcga tcagctgcgc ccgatcgaag
     1441 acgtcgcagg caaccgcgat gccgccagcc tcgcggatcc gcgccgcgcc ctggccgccg
     1501 cgggtcgacc cgaccacctc gtggcctgcc gccgcgagct gacggaccag gggcaggccg
     1561 atcgcgccac tggctccggc gatgaagatc ttcatggacg cagcctatgt caccaattca
     1621 atacccttcc cgcgatgccc ttcttcatct gtcccagttg cgggaaccgc gagaacagcg
     1681 gcgaccggac cgccggtttc agcccgcgac cgaagggctg ctcgaagtgc gggttcgggt
     1741 tcgtcttcga actgctcgac gattactacc cggccccgaa cgcggcgttc ttcgtctgcg
     1801 accaggaagg gcgggtcatc gacgcgggcc gggggagttt ccagctgacc ggcctgaccg
     1861 acgaggacgt gatcggccgc ccggttcgcg aagtgctggg gctcgaatgg atcgaccccg
     1921 gtgacggcgg accggaagcc gacacaaacg gtgacggtga gaccgacccg atcgagactt
     1981 ccctggaatg gggtgtccgt tcgctcggaa agcgcgtcga agtgcgctcc gagaaggatt
     2041 ccgccgccca ggccattgct gacgtcttcc cggcgtatga cgatgacggg ggtctgatga
     2101 tcgtcttgac gccagaaggg taagcttccg ctcgaatggg aggcaggcgc cgcaacatat
     2161 tcgtgctgct gttcgtgctc ggtctggtga tcgtgtcggg catcgtgtgc ctgaagaagc
     2221 cgacccagct cgggctcgac cttcagggcg gcatcgagct gacccttcag ggtcggccga
     2281 ccccgcaggt caaggaagtc acttccgagg cgatggaccg ctcggtggac atcatccgtt
     2341 ccggttgcga caagctcggc gtttccgaaa tcgaggtttc gcgactgggc gccgaccaga
     2401 tctcggtcgg cattccgggc gcgaccaacg tcggtaccgc gaccgaatgc gcaaccaaac
     2461 ccgcccggct ttacttctat gactgggaga acaacctggt cggaccggcc cgtgacctca
     2521 gcctggtcgg gacgggagcc gacaccgcag aagtctcgaa gcaggtccgc aaggcctggg
     2581 tcgacgccgg ccgcccgccg accgaaggct tcaaccgcac ctggatcggc ctcggtgccg
     2641 aaccgacgat gtgggacgcc gtcaacctcg ccgccaagca gccacgcgac gacaagtgcg
     2701 aggactgcac ctccggtgac aagtactacc tcttcgacga gaagacccat gaactgatct
     2761 cggggccggt cacggcgaag gaagacctct actacaacga tcagggccgg ccgatccctc
     2821 acaagggcat cgtcaagaag gttccccagg gcacgatcat cgtcgacgag ctgccgaccg
     2881 attccgacgg caagacgatc gacgacgagt cgcaggccgg ctacttcgtc ctcaaggaca
     2941 atgcggccct ttccggatcc gagatcaagg atccccagca gaacctcgac cagttcaacc
     3001 agccgaatgt gaccttcgac ttcaccggcg acggcaagga ctccttccag gagatcaccc
     3061 gccggatcgc ccaggaaggg caggcccgcg cgatcggagg ggtcacctcg gccgaccagg
     3121 cttcggccct ttccggtcac ttcgcgatca tcctcgacgg ccaggtcgtc tcgaggccga
     3181 tcatcaactt cgtcgagaac ccggacggca tcgacggccg ccagggggcc cagatctccg
     3241 gcgggttcac ggtcgaccag gcgcagaacc tggccgagct cctgcagcgt ggtgccctgc
     3301 cgattgacct caagctgact tcgcagagcc aggtgtcggc gaccctcggg caggaagctc
     3361 tcgatcaggg cgtgctggcg ctgctggtcg ggctcttctg cgtgatcatc ttcctgatcg
     3421 tcttctaccg cttcctcggc ggtgtcgcct cgctggcgct gctggtctac gcggtcctct
     3481 tcctcgccct gatcaaggtg atcccgatca ccatgaccct gccgggcata gccggtctgg
     3541 tcttaaccat cggggtcgca gccgactcca acatcgtcat cttcgaacgc gtcaaggaag
     3601 agttgcgtgc aggaagatcc gtgcaatcgg cgattacgct cggttaccgg cgcgggatca
     3661 gcacgatcat cgacgccaac gtcgtcaccc tgctgaccgc cttcatcctc ttcgtcctcg
     3721 cgacctcggg catcaagggc ttcgccttca cgctcggtgt cggcaccatc gtttcgctcc
     3781 tgaccgcggt cgtcttcacc caggcgctgc tcggttcgat gtcgaacacg aagctgatgc
     3841 gctccgacaa ggcattcgcc ggcatggagg gcaaggagag atggcatttc gacttcatgg
     3901 gcagcgcgcc gaagttcttt gcgatctccg gcgtgatcct gctgatcggt gccggcgcgc
     3961 tgaccaccaa gggcctcaac ttcggcatcg acttcgagtc cggcacccgg atcgaggtgg
     4021 ccctgaacga ttcggcctca accgaggatg tccgcagcgt gctcagcccg ctcgggctcg
     4081 gcagcgccga catccagacc atcaccgaca acccgaccct cggtgacaac gccttccaga
     4141 tcgagagcga gaccctcgac ccgtccgagg tctcgaaggt cgacgacgcg ctgaacaagg
     4201 agttcggcgt aagtgccgac ggcttcaaca gcacctcggt cggtccgacg ttcggcgcct
     4261 cggtcgcccg cagcgcggcc cttgcgatct tcttctcgat gatcctgatc atgggatacg
     4321 tggcgatccg cttcggcggc aagtacacgg tcccggtcct gatcgcgatc gcccacgact
     4381 tcctgatcgc actcggaatc tatgcattgc tcgggttcga ggtctcgagt gccaccgtgg
     4441 cggcattcct gacgattctg
//
LOCUS       DAOQFI010000053         4457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8340_length_4457_cov_3.622672, whole genome shotgun sequence.
ACCESSION   DAOQFI010000053 DAOQFI010000000
VERSION     DAOQFI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8340_length_4457_cov_3.622672"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLE93_01640"
     CDS             complement(<1..263)
                     /locus_tag="PLE93_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNH85780.1"
                     /translation="MSSYVELHCHSSFSFLDGASSPLELAEKAAELGYPALALTDHDG
                     VWGSMEFAVACKGAGIRAITGTELTVEEGGRLFHLTLLVENETG"
     gene            complement(348..632)
                     /locus_tag="PLE93_01645"
     CDS             complement(348..632)
                     /locus_tag="PLE93_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85781.1"
                     /translation="MSASMRRATMVTSGTRPTRLYEPRPVEVASDAAGAPVQINDVDV
                     VQVREEWIVEDGWWTAAPISRWYFEVVLIDGRDLAVFRAFPSGQWFAQRA"
     gene            complement(635..1780)
                     /locus_tag="PLE93_01650"
     CDS             complement(635..1780)
                     /locus_tag="PLE93_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85782.1"
                     /translation="MLEPIALAPQHDGPQTVGEVSPAAHRLGVRPGMGPGEAVDICPE
                     LALITPDPTRASQIWEAVLGRIESTGAEVESVRDGEAFFDATGVELLHGGLEGVLDAV
                     SEKLGPNFLLAAAPTRLAALALAGRQPAGKSSGHGRVLPPEELAAFLDPLPLATLLDR
                     LPGPAGEVRRMLESMRRLGLGTLGDLVALPADAVADRFGPAGLAARDLALGLEGPIRP
                     RTPFEQITEWLELPDAASGIHLEGGLTILCDRLAGRMRAAGLSLRSLSVEARLLGGGS
                     WSHRASPRRPTTSSPVLRMLLFPALEQLSRPADRLGLRALETAPQAADQLEVIHRPGE
                     TRRRRLGEAARQVRAAVGESALMRILETEPESRLPERRMLLTPYSTE"
     gene            1965..3215
                     /locus_tag="PLE93_01655"
     CDS             1965..3215
                     /locus_tag="PLE93_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNH85783.1"
                     /translation="MGKEQRYIHRDPAWIRGMTQPRLSRRQVMKGAAGAAMSVSLAQL
                     IAACGLPGSRDTGWTAGTDWNAWWRDQQKNGSFNFASWPLYIDTDGGKHPSLDKFTKE
                     TGIDVTYSPVIQDNASFFSQISPVLQNGQSIGYDLIVISNGWQLTQLMQNKWLVPLDH
                     SRMPNFRKYAGTVATDLAYDPHLTYCAVWQAGVTAIAYDEKQVDGPIDSLDALFDPKY
                     KGKVGMLSDPDELGTAGLLALGINPQTSTYADWQKSADLLTRQRDEGIVRQYYDNSYI
                     KALEDGDLAISQAYSGDVFQAQNSGYPNLKFVIPKQGGVIWRDNMVIPYHAENPVDAI
                     EWINFAYNPEIAALIADWVNYICPVPAAKPIIANQLDDPTVANSPLVFPSEAELSRLK
                     EYPVTETLKTHEQWVGLFDPIIQS"
     gene            3244..4092
                     /locus_tag="PLE93_01660"
     CDS             3244..4092
                     /locus_tag="PLE93_01660"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH85784.1"
                     /translation="MKSRIGPYLQSAPAMLWLALFFIVPLLAIVSISLMTGNSIDGYT
                     LTWNFSVFPDALDQYSAQLGRSFLYGGISTLLALIAMYPVAYWIAFHGGRRKSIFLFL
                     LLLPFLVSFIIRILTWQFILSDEGIVLGTLKDLGILGENAHILSTPVAVITGLTYDAL
                     PWMALPIYVALEKIDRDLVEAAGDLYASGYQQFRRIILPLSAPGIYAGILLVGIANIG
                     DYLSAQILGGPSTTMIGNIIQTQYVQNGDYPVASALSLMLMLALLLCVFVYARVFGTR
                     TIQEYA"
     gene            4092..4436
                     /locus_tag="PLE93_01665"
     CDS             4092..4436
                     /locus_tag="PLE93_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85785.1"
                     /translation="MAGKGISSADSARPVRRFPWTRFVLPSYVFVVMAYTLVPIAVMI
                     LYTFNKAPNQRLTFSWHGFTTEWYSKLFEVQDLTQALWHSLEVAVISSIIATALGTCG
                     GPGRSTWWWSPT"
BASE COUNT          789 a         1532 c         1401 g          735 t
ORIGIN      
        1 ccggtctcgt tttcaaccag cagggtcagg tgaaagagcc ggccgccttc ctcgacggtc
       61 aactcggttc cggtgatcgc ccggatcccc gcgcccttgc aggcgaccgc aaactccatc
      121 gacccccaga ccccgtcgtg atcggtcagg gccagggccg ggtaacccag ctcggccgcc
      181 ttctctgcca gttccagcgg cgaagaagcc ccgtccagaa aggagaaaga cgaatggcaa
      241 tgcagctcga catacgacga catgtcatcc tcgggcccgg cgaggtcggg aggggcaccg
      301 agatggcaaa agtggccata tagcgctctc ttttgccatc tcgttcctca ggcccgctgg
      361 gcgaaccact ggccggaggg gaaggcgcgg aagacggcca ggtcacggcc gtcgatcagc
      421 accacctcga aataccagcg gctgatcggg gcggcggtcc accagccgtc ctcgacgatc
      481 cattcctccc ggacctggac cacgtccacg tcgttgatct ggaccggagc ccccgccgcg
      541 tcgctggcca cctcgaccgg tcgcggctcg taaagccggg tcggccgggt gcccgaagtg
      601 accatggtcg ctcttctcat cgatgcgctc accgctactc ggtcgagtag ggggtcagga
      661 gcatgcgccg ctcgggcagg cgggactcgg gctcggtttc caggatccgc atcagggcgc
      721 tttccccgac cgcggcgcga acctgacggg ccgcttcacc gagccggcgg cgacgggttt
      781 cgccgggacg gtggatgacc tcgagctgat cggccgcctg gggagcggtc tccagcgccc
      841 gcaggccgag ccggtcggcc gggcgggaaa gctgctcgag agcggggaag aggagcatcc
      901 gcaaaaccgg cgaggaggtc gtggggcggc ggggcgaggc ccggtgcgac cagctgccgc
      961 cgccgagcag ccgggcctcg accgaaagcg acctcaggct gaggcccgcc gcccgcatcc
     1021 tcccggccag gcgatcgcaa agaatggtca gcccgccttc gaggtggatg ccgctggccg
     1081 cgtcgggcag ctccagccat tcggtgatct gctcgaaggg ggtgcgaggg cgaatcggcc
     1141 cttccaggcc gagggccagg tcacgggcgg ccagcccggc ggggccgaaa cggtcggcga
     1201 ccgcgtcagc cggcagggcg acgagatccc ccagggttcc cagccccagc cggcgcatgg
     1261 attcgagcat ccggcgaacc tcgccggccg gcccgggcag gcggtcgagc agggtcgcga
     1321 gagggagcgg gtcgagaaag gcggcaagct cctccggcgg caggacccgg ccatgaccgg
     1381 aactcttgcc ggccggttgc ctgccggcca gggcgagggc ggccagacgg gtcggagcgg
     1441 cggcgaggag gaagttcggc ccgagctttt cgctgaccgc atcgagtacg ccttcgagcc
     1501 cgccgtggag cagctcgaca ccggtggcgt cgaagaaggc ttcgccatcc cggaccgatt
     1561 cgacctccgc accggtcgat tcgattcgcc ccaggacggc ttcccagatc tggctggccc
     1621 gggtcggatc gggagtgatc aaggcaagtt ccggacagat gtcaactgcc tcgccgggac
     1681 ccatgccggg ccttaccccc agccggtgtg cggccgggga aacctcgccg accgtctgcg
     1741 ggccatcatg ctgcggggcc agggcgatcg gctccagcaa gcggttttga cccttttcga
     1801 gggctgcccg gagagccaga aggggaagca ggacacaaac gaacatatac gaacatatgt
     1861 tcgcatatcc ggccgggcca ggtcaagcag atcgtcatac gaaaggggac tttccggact
     1921 tttcggccga ccggggcgcg aaagcggatc gctatcgtga atccgtgggc aaggagcaga
     1981 gatacatcca tcgtgatccg gcctggatca gggggatgac tcagcccagg ctttcccgcc
     2041 ggcaggtcat gaagggcgcg gccggcgccg cgatgagcgt cagcctcgcc cagctgattg
     2101 ccgcctgcgg tctgcccggc agccgcgaca ccggctggac ggccggcacc gactggaacg
     2161 cctggtggcg ggaccagcag aagaacggct ccttcaactt cgccagctgg ccgctttaca
     2221 tcgacaccga cggcggcaag catccttcgc tcgacaaatt cacgaaggaa acgggaatcg
     2281 acgtcaccta cagcccggta atccaggaca acgcttcctt cttctcccag atcagcccgg
     2341 tcctgcagaa cggccagtcg atcggctacg acctgatcgt gatctcgaac ggctggcagc
     2401 tgacccagct gatgcagaac aagtggctgg tcccgctcga ccattcgcgg atgccgaact
     2461 tcaggaagta cgccgggacg gtcgccaccg acctggccta cgacccgcac ctgacctact
     2521 gcgcggtctg gcaggccggg gtaacggcga tcgcctacga cgagaagcag gtcgacggtc
     2581 cgatcgattc cctcgacgcc ctcttcgacc cgaagtacaa gggcaaggtc gggatgctca
     2641 gcgacccgga cgagctcggt actgccggcc tgctcgctct cggcataaat ccgcagacct
     2701 cgacctacgc ggactggcaa aagtccgccg acctgctgac ccgacagcgc gacgagggga
     2761 tcgtccgcca gtactacgac aacagttaca tcaaggccct cgaagacggc gacctggcga
     2821 tcagccaggc ctattcgggg gatgtcttcc aggcccagaa ctccggctat cccaatctca
     2881 agttcgtgat cccgaagcag ggcggggtga tctggcggga caacatggtg atcccgtacc
     2941 acgccgaaaa cccggtcgac gcgatcgagt ggatcaactt cgcctacaac ccggagatcg
     3001 cagccctgat cgcggactgg gtcaattaca tctgcccggt gccggccgcc aagccgatca
     3061 tcgccaatca gctcgacgat ccgaccgtgg ccaacagccc gctcgtcttt ccatccgaag
     3121 ccgagctttc ccgactcaag gaatacccgg tgaccgagac tctcaagacc catgagcaat
     3181 gggttggact cttcgacccg atcatccagt catgagcgcc ccggccagca agcggaggtc
     3241 acagtgaagt cccggatcgg gccgtacctg cagtcggcac cggccatgct ctggctggcg
     3301 ctcttcttca tcgtgccgct gctggcgatc gtctcgattt cgctgatgac cggcaattcg
     3361 atcgacggct acaccctgac ctggaacttc tcggtctttc ccgacgcgct cgaccagtac
     3421 tcggcccagc tcggccgctc cttcctctac ggcgggatct cgaccctgct ggccctgatc
     3481 gcgatgtatc cggtcgccta ctggatcgcc tttcacgggg gcaggcgcaa gtcgatcttc
     3541 ctcttccttc tgctcctgcc cttcctggtc agtttcatca tccgcatcct gacctggcag
     3601 ttcatcctct ccgacgaggg gatcgtgctc ggcaccctga aggacctcgg gatccttggc
     3661 gagaacgccc acatcctttc gactccggtc gcggtgatca ccggactcac ctatgacgcg
     3721 ctgccctgga tggcgctgcc catctacgtc gccctggaga agatcgaccg cgacctggtc
     3781 gaagcggccg gtgacctata cgcctccggc taccagcagt tccgacggat catcctgccg
     3841 ctctcggccc cgggcatcta cgccggcatc ctgctggtcg ggatcgccaa catcggcgat
     3901 tacctgagcg cccagatcct cggcggtccg agcaccacga tgatcggcaa catcatccag
     3961 acccagtacg tgcagaacgg tgactacccg gttgcctcgg ccttgtcgct gatgctgatg
     4021 ctggcgctct tgctttgcgt cttcgtctac gcccgggtct tcggcacccg gaccatccag
     4081 gagtacgcct gatggccggc aagggcatct cctccgccga cagcgcccgc ccggtccgcc
     4141 gcttcccctg gacccgcttc gtcctgcctt cctatgtctt cgtggtgatg gcctacacgc
     4201 tggtcccgat cgcggtgatg atcctctaca ccttcaacaa ggcgccgaac cagcggctca
     4261 ccttctcctg gcacggcttc acgaccgagt ggtactcgaa gctcttcgag gttcaggacc
     4321 tgacccaggc tctctggcac tcactcgaag tggcggtgat cagttcgatc atcgccaccg
     4381 cgctcggaac ctgcggcggg ccagggcgat cgacctggtg gtggtcacca acctgaccgc
     4441 cccgaccgtg gtggtcg
//
LOCUS       DAOQFI010000054         4446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8379_length_4446_cov_3.988841, whole genome shotgun sequence.
ACCESSION   DAOQFI010000054 DAOQFI010000000
VERSION     DAOQFI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8379_length_4446_cov_3.988841"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLE93_01670"
     CDS             <1..837
                     /locus_tag="PLE93_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85786.1"
                     /translation="VGETILDGTPNQFTNESTAYFDFHSTEPGSYFECDGGDDDWLLC
                     SSPATIVQVSPGVSTFRVRATDSAGNVDPTPATFEFEMDQIKPETTIDSGPSGTITTS
                     QADFAFSSNKSPAQFFCSIDGADAEACASTKTYTGLSDGQHEFRVYARDKFGNQDSTP
                     DSRTFTVDTSGPVDPPSNAFTFGRLKLNTKQGTATLQVKVPGAGKVQLLGSKTVAASS
                     KSARAKSTVTLAVKARGKATKTLKKRGKVGLAVKVRFTPTGGTAKTMTKTVKLVRKKT
                     GK"
     gene            948..1880
                     /locus_tag="PLE93_01675"
     CDS             948..1880
                     /locus_tag="PLE93_01675"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNH85787.1"
                     /translation="MICGIAFLLSGAIGEAGFIPKLDRVKFDDGEVYRQGCIVGDGQL
                     RSRPCRFGYRNSKRRVVLFGDSHAAEWGPALLRLARADHWQVIVLTRASCPAALTNID
                     RYCNTWRHNSLKRIRRLRPGLVVVASAANYHHYWTLRKRRGLDRQASERPLVHGMIRT
                     LRYLKRWSRKVVLIRDHSAAPFSVTDCLRRNPTRPQRCDFRSTRRKAWSYEFKAAGRV
                     DGVGVIDPQPMLCPGRRCYAVDGRHLVYRNYGHLAAAWVRTRNRWLGRNLNDPWNGRD
                     VKPVLSNDSSDERSRPGYLVRSIPSPMNGQATSD"
     gene            complement(1915..2226)
                     /locus_tag="PLE93_01680"
     CDS             complement(1915..2226)
                     /locus_tag="PLE93_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85788.1"
                     /translation="MGVAACAVLLIAGCGGVAELDSSETDTVNAANSAVADATASGSL
                     KPGDQAKLEALMVLCHEKPLAESDGESMRELITRLAPELKSADPKMSRRLKRFATDGC
                     N"
     gene            2354..2569
                     /locus_tag="PLE93_01685"
     CDS             2354..2569
                     /locus_tag="PLE93_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85789.1"
                     /translation="MGVQTSKTPHTVRLEEASRRAHEAEANGDDAAAIAAWREYRLVR
                     DAGRPAEDLLEEGITLSKMADRLIEPR"
     gene            2566..3042
                     /locus_tag="PLE93_01690"
     CDS             2566..3042
                     /locus_tag="PLE93_01690"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNH85790.1"
                     /translation="MTDFGRILKDLNAAGVRYVVVGGIALIRHGVVRATRDVDVVYDP
                     DPSNLEAIRSLAEKWGATRPDGSPLLENAFSAGRSVHLSTPLGEIDLLSEETASVSFV
                     NLLERSEIRRIDAVEAPICSLADLVALKRAAGRERDLADLSDLEATHGSLPARPED"
     gene            complement(3131..3219)
                     /locus_tag="PLE93_01695"
     tRNA            complement(3131..3219)
                     /locus_tag="PLE93_01695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3183..3185),aa:Ser,seq:tga)
     gene            complement(3285..3464)
                     /locus_tag="PLE93_01700"
     CDS             complement(3285..3464)
                     /locus_tag="PLE93_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85791.1"
                     /translation="MSKMGEVESEEEIRKEMEELGVSNDATGSPDVLMSGPMIQVTVI
                     VLVVLLVAGALFLVF"
     gene            3503..>4446
                     /locus_tag="PLE93_01705"
     CDS             3503..>4446
                     /locus_tag="PLE93_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH85792.1"
                     /translation="MRRLLFLSCAVVFLDVTFFSVLTPLLPSYKSDHHLSEGAIGVLA
                     GAFAAGSLVMAVPGGWLVARVGARKTVIAGLTGIGICSPLFGFAEHIVLLDLLRFLQG
                     GCGALMWAGAISWVVVAAPTARRGELLGIVIAAAVVGELLGAPLGAIAHQVGTEPVFS
                     MVLVAALILITIAVTLPEPTGVESQPLGEAWRRIRQSDVPSGVLMLAGPSVAFGLVVV
                     IGPLRMDDLGASPFLIAAAFASGSVVEAVVGPVIGRISDRVGRTLPYLIGAGALILAL
                     VGLGIFDRLSLLFAVVMVMSFGAGMAFTPASTLVTDTAT"
BASE COUNT          755 a         1436 c         1430 g          825 t
ORIGIN      
        1 gttggtgaaa cgatcctcga cggtaccccg aaccagttca ccaacgaatc caccgcctac
       61 ttcgacttcc attcgaccga acccggttcc tacttcgagt gcgacggcgg cgatgacgac
      121 tggcttctct gcagctctcc ggccacgatc gtccaggtca gcccgggagt cagtacgttc
      181 agggtccgcg cgaccgacag cgccggcaac gtcgacccga ccccggcgac tttcgagttc
      241 gagatggatc agatcaagcc ggaaacgacc atcgactcag gtccttccgg aacgatcacc
      301 acaagccagg cggacttcgc cttcagctcg aacaagtcgc cggcccagtt cttctgcagc
      361 atcgacggtg ctgatgcgga agcctgcgcc tccacgaaga catacacagg cttgtctgac
      421 ggccagcacg agttcagggt ttacgcccgg gacaagttcg gcaaccagga ctcgaccccg
      481 gactcccgga ccttcacggt cgacacctcc ggtccggtcg atccgccttc gaacgccttc
      541 accttcggca ggttgaagct gaacactaag cagggcaccg cgaccctcca ggtcaaggtg
      601 ccgggcgccg gcaaggtgca gctgctcggc tcgaagactg ttgccgccag ttcgaagtcg
      661 gcccgggcga aatcgaccgt caccctggcc gtcaaggcca ggggcaaggc gacgaagacc
      721 ctcaagaaga ggggcaaggt cggcctcgcg gtcaaggtcc ggttcacccc gaccggtggc
      781 accgcgaaga ccatgacgaa gaccgtgaag ctggtcagga agaagaccgg aaagtagcgc
      841 cggtgacatc cccgggcatg agtacttctt gacttggaga gtcaggatta tgcttgaatg
      901 ggaagcggtt tttcatggtt gggcacggtc gaatttctct ggctgcggtg atctgcggca
      961 tcgcgtttct gttgtcaggc gccattggcg aggcgggctt catcccgaag ctcgaccgcg
     1021 tcaaatttga cgacggcgag gtctatcgtc agggctgtat cgtcggcgac gggcagctca
     1081 ggtccaggcc ctgccggttc ggctaccgga actcgaaacg gagggtggtc ctgtttggtg
     1141 attcgcacgc cgccgagtgg ggtcctgccc tgctcaggct ggcccgggcc gaccactggc
     1201 aggtgatcgt cctgacccgg gccagctgcc cggccgccct gaccaacatc gaccgctact
     1261 gcaacacctg gcggcacaac tccctgaagc ggatcaggcg tttgcgaccc ggtctggtcg
     1321 tggtcgcctc ggccgcgaat taccaccatt actggaccct tcgcaagcgc cgtgggctgg
     1381 accgccaggc gagcgaacgc ccgctggtcc acggcatgat caggaccttg aggtacctga
     1441 agcgctggtc gagaaaggtc gttctgatcc gggaccactc ggccgcgccg ttcagcgtca
     1501 ccgactgcct gcgcaggaac ccgacccgcc cgcagaggtg tgatttcagg tcaacccgca
     1561 ggaaggcctg gtcatacgaa ttcaaggcgg ccggaagggt ggacggtgtc ggagtgatcg
     1621 atccgcagcc gatgctatgc cccggccgcc ggtgctacgc ggtcgacggc cgccacctgg
     1681 tctaccgcaa ttacggccac ctggcagcgg cctgggtccg gacccggaac cggtggctcg
     1741 gccggaacct gaacgatccc tggaacggaa gggacgtcaa gccggtcctt tcgaacgatt
     1801 cgtccgatga acggtcacgg cccgggtacc tggtccggtc gatcccctcc ccgatgaacg
     1861 gccaggcaac cagcgattga gcgacccggt tccggctggc cggccgggga gcgctcagtt
     1921 gcagccgtcg gtggcgaacc gcttgaggcg ccgggacatc ttcggatcgg cactcttcag
     1981 ctccggtgcg agccgggtga tcagctcgcg catcgactcg ccatccgatt ccgccagggg
     2041 tttctcgtgg cagagaacca tcaacgcttc gagcttcgcc tgatccccgg gtttcagcga
     2101 tccgctcgcg gtggcatccg ccacggccga gttggcggcg ttcaccgtgt cagtctcgct
     2161 ggagtcgagc tccgcaactc ctccgcaacc ggcgatcaac agcaccgcgc atgcggcaac
     2221 tcccaaagct ctcctcacat caacctccgg acgggatcaa ggacgggcga agcctacatt
     2281 cgcaagccgt ccagcgggac tcttgccggc cgaaacccac atgaaaccga cgctgtcggg
     2341 ctatccttga cccgtgggcg ttcagaccag caagactcct cacaccgttc gcctggaaga
     2401 ggcgtctaga agagcgcatg aggcggaggc aaacggcgat gacgcggctg ctatcgcggc
     2461 ctggcgcgag tatcgcctcg ttcgtgacgc tggccgtccc gccgaggatt tgcttgagga
     2521 gggaatcact ctgagcaaga tggccgatcg gctcatcgag ccgcggtgac cgactttggt
     2581 cgcatactca aggacttgaa tgctgcaggg gtccggtacg tggtagttgg cggtatcgcg
     2641 ttgatccgtc acggtgtggt gcgcgccacc cgggatgtgg atgttgtcta tgaccctgac
     2701 ccgtccaatc tggaggcgat tcgttcgttg gcagagaaat ggggtgcgac ccgacccgat
     2761 gggtcacctc tcctggagaa cgccttctcc gctggccggt cggttcatct gtcgacgcca
     2821 ctgggcgaaa tcgacttgct ttcggaagag acggcctcgg tgagctttgt caacctgctg
     2881 gagagatccg agatccgccg catcgacgct gtcgaagccc cgatctgttc acttgccgac
     2941 ctggttgccc tgaagcgcgc agcaggcaga gagcgcgatc tcgcggatct ctcggatctc
     3001 gaagcaactc atgggagctt gccagcccgg cccgaggact gaagcacgcc cgagcttcgg
     3061 cttcgagatt ggcagaatca cagaagcatc ctgccaggcc cgcaccaaga gcgagatttg
     3121 tggtgtggag cggaggggga gggattcgaa ccctcgatac gccgtaagac gcatgctggt
     3181 tttcaagacc agtgcattca accgctctgc cacccctccg tggcctgttc ggcccgggga
     3241 ttctgacagg ccgcgggtcg attggcggga ttgagccgcc gcggctagaa gacgaggaag
     3301 agcgcgccgg cgacgaggag gacgacgagg acgatcaccg tcacctggat catcggtccc
     3361 gacatcagga catccgggga gccggtcgcg tcattgctga ctccgagctc ctccatctct
     3421 ttgcggatct cttcctcgga ttcgacttca cccatcttgc tcatgccgta actatcccac
     3481 gaccgcctgt caggatgggg tcgtgcgtcg tctcctcttt ctttcctgtg cggtcgtctt
     3541 ccttgacgtc acattcttct cggtactgac cccgctgctt cccagctaca agagcgacca
     3601 tcacctcagc gaaggggcga tcggggtgct ggccggtgca tttgccgccg gttcgctggt
     3661 catggcggtt ccgggcggct ggctggtggc cagggtggga gcgaggaaga ccgtgatcgc
     3721 cggcctgacc gggatcggaa tctgcagtcc gctcttcggg ttcgccgagc acatcgtgct
     3781 gcttgatctg ctccgtttcc tccagggcgg ctgcggcgcg ttgatgtggg cgggcgcgat
     3841 cagctgggtc gtcgttgcgg ccccgaccgc gcgccggggg gagctgctcg ggatcgtgat
     3901 cgcggccgcg gtggtcggcg agctgctcgg ggcgccgctt ggggcgatcg cacatcaggt
     3961 cggcaccgag ccggtcttca gcatggtcct ggtcgcggcc ctgatcctga tcacgatcgc
     4021 cgtgaccctg cccgagccga ccggtgtcga gtcccagccc ctcggcgagg cctggcgccg
     4081 gatccgccag tccgacgtgc cgagcggagt cctgatgctg gccggacctt cggtcgcctt
     4141 cgggctggtg gtggtgatcg gcccgctccg catggatgat ctcggagcgt cgccgtttct
     4201 gatcgccgcc gccttcgcat ccggctcggt cgtcgaagcg gtggtcggtc ccgtgatcgg
     4261 gcggatctcg gaccgggtcg gccgcaccct gccctacctg atcggggcgg gtgccctgat
     4321 cctcgccctg gtcggcctcg ggatattcga ccggctttca ctgctcttcg cagtggtgat
     4381 ggtgatgtcc ttcggggccg gaatggcctt caccccggcc agcacgctgg tcaccgacac
     4441 agcaac
//
LOCUS       DAOQFI010000055         4434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8421_length_4434_cov_4.366065, whole genome shotgun sequence.
ACCESSION   DAOQFI010000055 DAOQFI010000000
VERSION     DAOQFI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8421_length_4434_cov_4.366065"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLE93_01710"
     CDS             <1..305
                     /locus_tag="PLE93_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85793.1"
                     /translation="VAPKKRNLKAGRKFNLVVKVTNFITATADAKNVKVTVKSSNKRV
                     KVKSAVIRKISPGKTATVKIPVRALGKARGKSKITVTAAGNKAYSNIKVIPKKKRR"
     gene            complement(302..946)
                     /locus_tag="PLE93_01715"
     CDS             complement(302..946)
                     /locus_tag="PLE93_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HNH85794.1"
                     /translation="MSRRVSSFIACALIGGSALVLAVPAAAHIQVEPTEVAPGDAALF
                     TLLVPGESAAGTSEVKLKVPPGVYPFSYEETPGWKRKLVKKPNGMVDQVIWTGTAAPD
                     GLVRFSFLAGTPEKLGEIKWAAIQNYANGEESRWIGMPESENPAPVTIVSDSVPRQNA
                     GGESEVAHEHASPTSSENATTDSETDWPITIAALLGFFFGLSSLVILLVNRINS"
     gene            complement(951..1316)
                     /locus_tag="PLE93_01720"
     CDS             complement(951..1316)
                     /locus_tag="PLE93_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNH85795.1"
                     /translation="MDSITSSNTVRGLVAGTIVAAAMILGGPAGQAEAHTGVTSTSAN
                     SRMLRVFFAAPIRRGTLRARNASGAIVSKGRGGRDPRNINALRVGLRNLRGGYYRATW
                     SIVGLDGHHQSGTVRFRVR"
     gene            complement(1319..2536)
                     /locus_tag="PLE93_01725"
     CDS             complement(1319..2536)
                     /locus_tag="PLE93_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme enzyme"
                     /protein_id="HNH85796.1"
                     /translation="MGRDRNKSNGRFAVPRRFTTRAMALAAIFLAVSAAAFGLQATGA
                     LKAPNNDWSWALPRNFPTPRVPARNPMSQGKVNIGHRLFYDVRLSGNQTQSCSSCHLP
                     ELAFTDGKTTSVGSTGDVLLRNAPSVANSAYHRTFTWANPALTSLESQMNVPLFGTGP
                     VEMGVNDRNKGKILRRIKSDPWYRKRFPKAFRWSKKPVNWFNIVRSISAFQRSIITGT
                     SKYNRFLRGKAELSGSERSGLNLFMGEEGECHHCHGSFIFDDQATYAGGPLEKLKFHN
                     TGLYNIGGTGAYPTGNRGLYEITGKKKDMGAFRAPSLLNVAKTAPYMHDGSVKTLMDV
                     VDIYARGGRNITEGPFAGDGATNPHKDPLISGINLSEQDKKDIVAFLKTLSERDYSKD
                     PRFWDPFKHKSRK"
     gene            complement(2539..3543)
                     /locus_tag="PLE93_01730"
     CDS             complement(2539..3543)
                     /locus_tag="PLE93_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallo-mystery pair system four-Cys motif
                     protein"
                     /protein_id="HNH85797.1"
                     /translation="MTIAHFRCVIVDEQLENNMKSSKKLGAIFAALALGLVGLIALPV
                     TANAHGGHAKKSKTQKVAIGFKAVNGNTPVSCSKPITGLGTTSRTAKLTDLRFYVSGV
                     QLLRKGGGAVRVKLQGGSKWSYTKGKDAVTLIDLENGAGACSAEGTKATNAFVRGTVP
                     KGRYVGVRYSVSVPDSLSHTDLTAMPSPLNNTAMGWSWQYGRKFMKIELGQGDTPDWA
                     SPIYYLHLGSTNCTGDPAAGEQAKCDLPNQNLITFRKFNPAGQRIAIDFKKLFSGVNV
                     AGESMDMGDGMDMGGMMAMGGCMSATDSPDCDPLFETLGLKLGSTKRTNQTAFRVVRK
                     "
     gene            complement(3629..3946)
                     /locus_tag="PLE93_01735"
     CDS             complement(3629..3946)
                     /locus_tag="PLE93_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85798.1"
                     /translation="MSVISHVSRESKATAVVLLLAWLAVAALSVTVALIAAPAFALFF
                     VLSLGFFPGEQLIVKARELVSRPREWSRPVPIAAPVLPFSSRPVGLDLAFALAVRPPP
                     VLQ"
     gene            complement(4219..>4434)
                     /gene="aspA"
                     /locus_tag="PLE93_01740"
     CDS             complement(4219..>4434)
                     /gene="aspA"
                     /locus_tag="PLE93_01740"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937305.1"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNH85799.1"
                     /translation="NHAQHEKHAEATLATATAFNPYIGYDKASEIVKEAAASGRPLRE
                     VAIEKGVEEKVLDEALDHHKMANPHSA"
BASE COUNT          809 a         1350 c         1388 g          887 t
ORIGIN      
        1 ccgtggcccc gaagaagcgg aacctgaaag cagggcgcaa gttcaacctg gtggtcaagg
       61 tgaccaactt catcaccgcc accgcggacg cgaagaacgt caaggtgacg gtcaagtcat
      121 cgaacaagcg ggtcaaggtg aagtccgcgg tgatcaggaa gatcagccct ggcaagaccg
      181 ccaccgtgaa gatcccggtc cgcgccctcg gcaaggcccg cggaaagtca aagatcacgg
      241 taaccgccgc cggcaacaag gcttactcga acatcaaggt gatccccaaa aagaagcgtc
      301 gctaggagtt tattctgttg accagcagga tcaccagcga cgagagcccg aagaagaacc
      361 cgagcaatgc ggcaatggtg ataggccagt ccgtctccga atccgtggtc gcgttttcgg
      421 agctggtcgg cgaagcgtgt tcgtgagcga cctcgctttc gccgccggcg ttttgacggg
      481 gaactgaatc ggaaacgatc gtgaccgggg cgggattttc cgattccggc attccgatcc
      541 atcgtgactc ctccccgttc gcgtagttct gaattgccgc ccatttgatc tcacccagct
      601 tctccggcgt tccggccagg aagctgaaac gcaccagccc gtccggggct gccgttccgg
      661 tccagatcac ctggtccacc atcccgttcg gtttcttgac gagtttccgc ttccacccgg
      721 gagtctcctc gtaggaaaac gggtaaacgc ccggagggac cttcaacttg acttcggaag
      781 ttccagcggc agattcgccg ggaaccagca gggtgaagag tgccgcgtct cccggagcaa
      841 cctccgtggg ttcaacctga atgtgtgctg ccgcgggaac ggctaggacc agggctgatc
      901 cgccgatcag ggcgcaggca atgaaactgc tgacccgcct tgacaagggg ctacctcacc
      961 cggaagcgca cggttcccga ctggtggtgc ccgtcgagcc cgacgatgct ccaggtcgcc
     1021 ctgtaatagc cgccacgcag attccgcagt cccaccctca gcgcgttgat gttgcgtggg
     1081 tcacgtcctc cgcggccttt ggacacgatc gccccgctcg cgtttcgggc tctcagggtt
     1141 cccctcctga tcggggcagc gaagaaaacc cggagcatcc tgctgttggc ggaggtcgat
     1201 gtgacaccgg tgtgggcctc cgcctggccg gccggcccac cgaggatcat tgctgccgcg
     1261 acgattgtgc cggcgaccag accgcggacc gtgttgctgc tggtgatgct gtccatgttc
     1321 atttccttga tttgtgcttg aagggatccc agaagcgggg gtccttggag taatcgcgtt
     1381 cgctcaacgt cttgaggaag gcgacgatgt ccttcttgtc ctgttcactg aggttgattc
     1441 cggagatcaa cgggtccttg tgaggattcg tcgccccgtc accggcgaat gggccttcgg
     1501 tgatgttgcg gccaccgcgg gcatagatgt cgaccacatc catgagcgtc ttgaccgagc
     1561 cgtcatgcat gtaaggggcc gttttcgcca cgttgaggag cgacggtgcc ctgaaagcac
     1621 ccatgtcctt cttcttgccg gtgatttcgt agaggccgcg gttgccggtg gggtaggcgc
     1681 cggttccacc gatgttgtag aggccggtgt tgtggaactt gagcttctcg agcggcccgc
     1741 ccgcgtaggt cgcctggtca tcgaagatga agctgccgtg acagtggtga cattcaccct
     1801 cttcgcccat gaagagattg aggccgcttc gttccgaacc gctgagttcg gctttgccgc
     1861 gcaggaaccg gttgtatttc gaggtcccgg tgatgatgct gcgctggaat gcgctgatcg
     1921 agcggacgat gttgaaccag ttgacaggtt tcttcgacca gcggaatgcc ttcgggaagc
     1981 gctttcggta ccacggatca ctcttgatcc ttcgaaggat cttgcccttg ttccggtcgt
     2041 ttacgcccat ttcgaccggg ccggtcccga agagcggaac gttcatctga ctctcgagcg
     2101 aggtgagcgc cgggttggcc caggtaaagg tccggtggta ggccgagttg gcgaccgaag
     2161 gagcgttgcg gaggaggacg tccccggtcg atccgaccga agtcgtcttg ccgtcggtga
     2221 atgcaagttc gggaaggtgg caggaagagc aggactgggt ctggttgccc gacaggcgga
     2281 cgtcgtagaa gaggcggtga ccgatgttca ccttcccctg gctcatgggg tttctggctg
     2341 gcacccgggg cgtggggaag ttccggggca gagcccagct ccagtcattg ttgggcgcct
     2401 tcaaggcgcc agtggcctga agaccgaagg ccgcggcgct gacggcgagg aatatcgccg
     2461 ccagcgccat ggcccgggtc gtgaaccggc gcggaaccgc aaagcggccg ttgctcttgt
     2521 tgcggtcccg ccccacgact acttccggac gactcggaac gcggtctggt tcgtcctctt
     2581 ggtcgaaccg agcttcaacc cgagcgtctc gaagagcgga tcgcaatcgg gggagtcggt
     2641 ggccgacatg cagccgccca tcgccatcat gccgcccatg tccatgccgt cgcccatgtc
     2701 catggactca ccggcgacgt tgactccact gaacagcttc ttgaagtcaa tggcgatcct
     2761 ctggccggcc ggattgaact tcctgaaggt gatcaggttc tggttcggca ggtcacactt
     2821 cgcctgctcg cccgctgccg ggtccccggt gcagttggtg ctgccgaggt gcaggtagta
     2881 gatcggtgaa gcccaatccg gggtgtcacc ctgaccgagt tcgatcttca tgaacttgcg
     2941 gccgtactgc cacgaccagc ccatcgctgt gttgttcagc ggtgacggca tcgcggtcag
     3001 atcagtgtgg ctgagtgaat cgggaacgct gacgctgtag cggacgccaa cgtacctacc
     3061 cttgggaacc gttccccgga caaacgcgtt ggtcgccttg gtcccctcgg ccgaacaggc
     3121 gccagcaccg ttctcgaggt cgatcagggt caccgcgtct ttgcccttgg tgtagctcca
     3181 ctttgaacca ccctgcaact tgaccctgac ggctccaccg cctttgcgca gaagctgtac
     3241 gcccgagacg tagaaacgaa ggtcagtcag ctttgcggtc cgggaggtcg ttcccaggcc
     3301 ggtgatcggc ttgctgcaac tgaccggcgt gttgccgttc actgccttga agccgatcgc
     3361 cactttttgg gttttgctct tctttgcatg tccgccatgg gcgtttgccg tgacggggag
     3421 ggcgattagg ccgaccaagc caagggccag ggcggcgaaa atcgcgccaa gtttcttgct
     3481 gctcttcatg ttgttctcca gttgttcgtc gacgatcacg caccggaagt gcgcgatcgt
     3541 cacgggaccc gcctgtgacg atcaagccgc agcgagttcc cgcttgattg atgcctcctt
     3601 caccttcagg tgaagaggcc ctcagtcact actgaagtac cggcggcggt ctgaccgcca
     3661 gagcgaaagc caggtccagg ccaaccggcc tggaagaaaa aggcagcacg ggcgccgcga
     3721 tcggaacggg ccgggaccat tccctcggcc tcgaaaccag ttccctcgcc ttgacgatca
     3781 actgttcccc ggggaagaac ccgaggctca aaacgaagaa gagcgcgaag gctggagcgg
     3841 cgatcagggc gacggtcacc gaaagcgctg ccactgccag ccacgcgagc agcaggacga
     3901 cagcggtcgc cttgctttca cgcgaaacgt gagaaatcac actcatcagc tactcaaaca
     3961 gtaccgggag ccaaaaagtt tcgttcccgc ctgttacgcc gtggggtcgg gtgagcatcg
     4021 ccgggacacg acacgcgcgg cattcagccg cgctgtcatt gagcgtcccg gcgacgctca
     4081 cccgacccca cggctacatc aacccggtcg aacttcagag gtgccgcaca tccagcagca
     4141 tcacgctgca aaacgtgcgg cacctccgtt caacgtggac cacccccgcg ccgggccctt
     4201 cgacaaaacc ggttgcgact aggccgaatg ggggttggcc attttgtggt gatcgagggc
     4261 ttcgtcgaga accttttctt cgaccccttt ttcgatcgcc acctcgcgga gcggtcgtcc
     4321 tgaagcggcc gcttccttga cgatctcgct ggccttgtcg tagccgatgt aggggttgaa
     4381 tgcggtggcc gtggcgaggg tggcctcggc gtgcttttcg tgctgagcgt ggtt
//
LOCUS       DAOQFI010000056         4409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8509_length_4409_cov_3.580386, whole genome shotgun sequence.
ACCESSION   DAOQFI010000056 DAOQFI010000000
VERSION     DAOQFI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8509_length_4409_cov_3.580386"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLE93_01745"
     CDS             <1..732
                     /locus_tag="PLE93_01745"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH85800.1"
                     /translation="GVAGRKPIHLLEPDQRKKVVQLKEMHIDIGADDREDAQKMVSVG
                     DPVVIDCEPQAIAGGRLVSRSMDNRLGSYVALEAARRIHENARAKVRFAGVAAVQEEI
                     GLFGSRTAAFTFRPDLAVAIDVTHATDAPGIDEKEVGSHPFGSGPVIGRGSTLSPKVY
                     ELLRDAAEKAGIDYSVGASGNSTHTDADSLQISRTGIPTGLVSIPLRYMHSPVEMVDL
                     ADVEATVALMVAFVEGLAVDVDLSR"
     gene            908..3625
                     /locus_tag="PLE93_01750"
     CDS             908..3625
                     /locus_tag="PLE93_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85801.1"
                     /translation="MNHQLGITNARRFVALAFALVMAFALTAAFANSQAEAGKKKKKA
                     KPATVKVVNITTANQQALLKQKKLTVKVKSTGKASPKVSAVSSGKSNYFKSVTVKFKK
                     KGTKTVSLALTSAGKTALGTCGAKTVQVNASYKKGKKKAKASKKKTLGKWADNSNCFV
                     TVDVGDDPAHCDFLDATQCLQPWPNDYYTKEDSSTPTGKRLNLGAESTPMNTGNATPV
                     HLDVTDINRGDGFSPGNEILIKIPGLDTPAAFTNSNLVPLDDISAYKDAAQAVIVIDA
                     ATGERQPIWAELDSNPTSVNPTEEDPGGININPTNTQPVNLIVHPAKNFEFGHRYIVA
                     FRNLKDADNNAIQSPLGFRVYRDNLKTKQSVVENRRSHMNSVINDLVNKAGVDRSSLY
                     MAWDFTVASEDSVTGRATTIRDDAFARLGDNNLADRQIAGNSPDIDVLAYCDKSNVAA
                     PSCGNNYPGIPEFNGSNPVTSPVPGTDIQRTVVGYIRDVPCYLDQDGCPASGSQFAFD
                     SNGNLTWNNTFKMDVPFRCMIPSSIVNTGTVVPGHTGTYGHGLLGSLNQINATVEAGN
                     RTNTVFCAANWDGFSTLDAGVVIQSLMNMSNFNKLVDRMQQGFVNFLYLQRALVHPDG
                     FADQAAFQMDHNGATPITPGSAIDTSAGAATRGYYYGISQGGIMGGALTALSPDVDKG
                     VLGVPGINYSTLLSRSVDSDEYFQAPGYGLYPNYPDMSSRQLLLSLIQLLWDRGEGNG
                     YANNMTDNPLPNTNSHEVLLQLALGDHQVTNYAAEVEARTIGAVRYAPTLRPERTWDL
                     DYEALPGNTSFPIGPNESFMVYYDGGPPSFNGTQDPGTAKPPLENVPPRTEWGFGEDP
                     HGFPRRALDANLHIKSFYENGTIDSCEATSPSPGDAYCYANGWDGVTGL"
     gene            complement(4004..>4409)
                     /locus_tag="PLE93_01755"
     CDS             complement(4004..>4409)
                     /locus_tag="PLE93_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNH85802.1"
                     /translation="CTRTFATGPESRLDPLAREIYEVTLRANEAGLGAVKAGAIGSEV
                     DEVARTLIRDAGYGENFGHGLGHGVGLEIHEAPRLGPSSKDELMAGEIVTVEPGIYIS
                     GATGVRIEDLVVVGDRGVDRNLSTVTKALTFV"
BASE COUNT          880 a         1508 c         1287 g          734 t
ORIGIN      
        1 ggcgtcgccg ggcgcaagcc gatccacctg ctcgagccgg atcagcgcaa gaaggtcgtc
       61 cagctgaagg agatgcacat cgacatcggc gccgatgacc gcgaggacgc gcagaagatg
      121 gtcagcgtcg gagacccggt cgtgatcgac tgcgaaccgc aggcgatcgc cggaggacgc
      181 ctcgtttccc gctcgatgga caaccggctg gggtcctatg tggcgctcga ggcggcccgg
      241 cggatccacg agaacgccag ggcaaaggtt cgattcgccg gcgtcgccgc ggttcaggag
      301 gaaatcggcc tgttcgggtc ccgcaccgcc gccttcacct ttcgacccga tctcgccgta
      361 gcgatcgacg tcacccacgc gaccgacgct ccaggcatcg acgaaaagga agtcggcagt
      421 cacccgttcg ggtcgggacc ggtgatcgga cggggttcga ccctctcccc caaggtctac
      481 gaactgctcc gcgacgccgc ggaaaaggcc ggtatcgatt actcggtagg cgccagtgga
      541 aactcgaccc ataccgatgc cgactctctt cagatttccc ggaccggcat cccgacgggt
      601 ctcgtctcga tcccgctgcg ctacatgcat tcgccggtcg agatggtcga cctggccgac
      661 gtcgaggcaa cggtggcgct catggtcgcc ttcgtcgaag gcctcgcggt cgatgtggac
      721 ctctcgcgct gaacccttct ttcggtacgg aatcgttcag aaacatcctg tccgatcggc
      781 gcaacttccg ccatgaatcc ggtctagtat ctgtgagtca agtcacacga ccggtgttgc
      841 cggtcggcca caaatcgcgg ccggtgttcc agccggtcaa agctatgaga gggagttacc
      901 cggagagatg aatcaccaat tgggaataac gaatgcacgc cgtttcgtgg cgctcgcgtt
      961 cgccctggtc atggccttcg cgctgacggc agcatttgcc aacagccagg ccgaggcagg
     1021 aaagaagaag aaaaaggcca agccggccac cgtcaaggtc gtcaacatca cgaccgcgaa
     1081 ccagcaggcc ctgctcaagc agaagaagct gacggtgaag gtcaagtcga ccggcaaggc
     1141 ttcaccgaag gtttccgctg tgagctccgg aaagagcaac tacttcaagt cggtcaccgt
     1201 caagttcaag aagaagggca ccaagaccgt gagtctcgcc ctgacttctg ccggcaagac
     1261 cgccctcggc acctgtggcg ccaagactgt ccaggtcaac gcctcataca agaagggcaa
     1321 gaaaaaggcc aaggcttcga agaagaagac cctcggcaag tgggctgaca acagcaactg
     1381 cttcgtgacg gttgacgttg gcgacgatcc cgcgcactgt gacttcctcg atgcgaccca
     1441 gtgcctccag ccctggccga acgattacta caccaaggaa gattcgtcga ccccgaccgg
     1501 caagcgcctg aacctcggcg ccgagtcaac gccgatgaat accggcaacg ccaccccggt
     1561 ccatctcgat gtcaccgaca tcaaccgcgg tgacggcttc agccccggca acgagatcct
     1621 gatcaagatt ccgggcctcg acactccggc tgctttcacc aacagcaacc tggttccgct
     1681 tgatgacatc agcgcctaca aggatgcggc ccaggccgtg atcgtgatcg acgccgccac
     1741 cggcgagcgt cagccgatct gggccgagct cgactcgaac ccgacctcgg tcaacccgac
     1801 cgaagaggat ccaggcggaa tcaacatcaa cccgaccaac acccagccgg tcaatctgat
     1861 cgtccatccg gccaagaact tcgagttcgg ccaccggtac atcgtcgcct tccgaaacct
     1921 gaaggacgcc gacaacaacg cgatccagtc gccgctcgga ttccgggtat accgggacaa
     1981 cctgaagacc aagcagagcg tcgtcgagaa ccgccgttcc cacatgaact cggtcatcaa
     2041 cgacctggtc aacaaggccg gcgttgatcg ttccagcctc tacatggcat gggacttcac
     2101 cgttgcctcc gaggacagcg tgaccggccg cgccacgacg atccgtgacg acgccttcgc
     2161 gcgtctcggc gacaacaatc tcgccgatcg ccagatcgcc ggcaacagcc cggacatcga
     2221 cgtcctcgct tactgcgaca agagcaacgt cgcggctccg agctgtggca acaactaccc
     2281 cggcatcccc gagttcaacg ggtcgaatcc ggttacctcg ccggtaccgg gcaccgacat
     2341 ccagcgcacg gtcgtcgggt acatccgcga cgtgccctgc tacctggatc aggatggctg
     2401 ccccgcgagc gggtcgcagt tcgccttcga ctcgaatggc aacctgacct ggaacaacac
     2461 cttcaagatg gacgttccct tccggtgcat gatcccgtcg tcgatcgtga acacgggcac
     2521 cgtggttccg ggtcacaccg gcacctacgg tcacggtctg ctcggatcgc tgaaccagat
     2581 caacgcgaca gttgaagccg gaaaccggac caacacggtt ttctgcgctg ccaactggga
     2641 tggcttctcg acgctggacg ccggcgtggt cattcagtcg ctcatgaaca tgagcaactt
     2701 caacaagctg gtcgaccgca tgcagcaggg cttcgtgaat ttcctgtacc tgcagcgggc
     2761 gctggtccac ccggacggtt tcgccgacca ggccgccttc cagatggatc acaacggagc
     2821 caccccgatc acccccggct ccgcgatcga cacgagcgcc ggtgctgcca cccgtggcta
     2881 ctactacggg atcagccagg gcggcatcat gggtggtgcg ctgaccgcgc tctcgcccga
     2941 tgtcgacaag ggcgtgctcg gagtcccggg catcaactac tcgaccctgc tcagccgctc
     3001 ggttgactcg gacgagtact tccaggcccc gggttacggg ctctacccga actaccccga
     3061 catgtcgtcc cgccagctgc tgctctcgct gatccagctc ctctgggatc gcggtgaagg
     3121 caacggctac gcgaacaaca tgaccgacaa cccgctgccg aacaccaact cgcacgaggt
     3181 tctgctccag ctggcgcttg gcgaccacca ggtcaccaac tacgcggctg aagtcgaggc
     3241 ccggacgatc ggtgcggtgc gctacgcgcc gaccctgagg ccggaacgga cctgggatct
     3301 cgattacgag gccctgccgg ggaacaccag tttcccgatc ggcccgaacg agagcttcat
     3361 ggtctactac gacggcggac cgccctcatt caacggcacc caggacccgg gcacggcgaa
     3421 gccgccgctc gagaacgtcc cgccgcggac cgagtggggc ttcggcgagg acccgcacgg
     3481 gttcccccgc cgggcgctgg acgccaacct ccacatcaag tcgttctacg agaacggaac
     3541 gatcgacagt tgtgaggcga cttcaccgtc gccgggcgac gcctactgct acgcaaacgg
     3601 ctgggacggc gtcaccggtc tctagccagc agcagaagca gcattcgaga acggggcccc
     3661 gcggggcccc gttttcgttg cacgagccgg ggtgggtcgg atttcccgtc ccgggactgg
     3721 aaatccgacc caccccggtg gaggcgaccc ggcccggtag aggcgaccca ccccggtgga
     3781 ggcgagctgg ctctgtgccg gcatccgggc tccgcctcgg ctgcggccgt tgatgcaatg
     3841 aaaagaccgg ccccctcagg ccggtctttt cgtctgcacc tctctcctca aaaactccct
     3901 cgccatattg ctctggagtt ccctccccac tcgtgacaga agcaatgtcg tctgtgtgac
     3961 gggtatcaca ctatagagga tcgttacaaa agatgcattc cccctacacg aaggtcaggg
     4021 ccttggtgac cgtgctcagg ttccggtcaa ctcccctatc cccgaccacg accagatcct
     4081 cgatcctcac accggtcgcg ccagagatat atatccccgg ttcgaccgtg acgatctcgc
     4141 ccgccatcag ctcgtccttg ctgctcggac cgagccgggg tgcctcgtgg atctccaggc
     4201 cgaccccgtg gccgagtccg tgcccgaagt tctcgccgta gccggcgtcg cggatcaatg
     4261 ttctggcgac ctcgtcaacc tcggacccga tcgcgccggc cttcaccgcg ccgagccccg
     4321 cctcgttggc tcgcagggtc acctcgtaaa tttcacgggc cagcgggtcg aggcgggact
     4381 ccgggccggt cgcaaaggtc ctcgtgcag
//
LOCUS       DAOQFI010000057         4385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8590_length_4385_cov_3.037413, whole genome shotgun sequence.
ACCESSION   DAOQFI010000057 DAOQFI010000000
VERSION     DAOQFI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8590_length_4385_cov_3.037413"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLE93_01760"
     CDS             complement(<1..557)
                     /locus_tag="PLE93_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNH85803.1"
                     /translation="MGKVVNIEESQKRRQSAKRQKRSRTALVLGGGGFTGGVYEIGTL
                     RALDLLAVNSTVNDFDMYIGTSAGSFIGSMLAAGVTPEEMMRVLTDEKGALLERISQD
                     TVLRPNFSEYAERIVTLPLHVAGMVKSMVPRFRDLSVIDIAYGLAEHLPSGMYSNAGI
                     GAYVREALATKGIVDVRLSDDGRRIL"
     gene            629..1969
                     /locus_tag="PLE93_01765"
     CDS             629..1969
                     /locus_tag="PLE93_01765"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNH85804.1"
                     /translation="MKHSLPLLAAVLSLALAAPSAAASPDPLPNPDRHFATPFMHRGE
                     KGASGPADASASAGRACQVMRMLARRGNGAVGLRVFNLRSRKNVCALNSGARRSLASN
                     NKLFTISTALDRLGQDQRLQTRVMAGGKTSRNGVLDGNLYLKGGGDPSLGTARFVNRW
                     FGGHGSEIEKLARKVRQAGIRKVTGRLFFDDSVFDQVRGVADSGYATSPWIGPLSGIA
                     FNEGYTSPSLSRFSNDPARLAARTFVRAMRARGVKIRPRFSPRDTPVKLLKRVVAREV
                     SPDMGWMARATLLDSNNFFAETLLKVLGASFHGLGTTANGVKVVRRTMASLKVDVWPI
                     DGSGLTYGNRSTAHDVVKLLFRARSEPWGKALVAGMPTAGVDGTLEDRMRGSAAQGNC
                     HAKTGTLTGVSALSGYCFNSSGRRFAFSILMNDVTNLDTAHRAQDRIAALIARL"
     gene            complement(2004..2930)
                     /locus_tag="PLE93_01770"
     CDS             complement(2004..2930)
                     /locus_tag="PLE93_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85805.1"
                     /translation="MSFSGSARRFLAVAGVAVFTIAVGSASATVPGKNGLLLVSGYTD
                     GGINGSTSRIFTETLTGKSKELLGALDQSYSDPAVSPNGKQIVYSVYPGYRMWLGSFA
                     KPKQAKAITPEESDVLNLETVFAPDGKSIYFSKKYYLDSGVFWHLKKYTFKTKKTTSY
                     KVDTKLDWGFSDVSPNGRYLAYNRGGDEDASKIRLLDTRTGKSHTVNTKGAALGPAFS
                     PDGKSMVYTAQYGDAWELVQSRLDGKNARRLTRTGAINFDPHYSPDGKMIAFTQGVEG
                     NKKIGILTLKTGKIRYLVATGDYAEVEQWLRK"
     gene            2962..3531
                     /locus_tag="PLE93_01775"
     CDS             2962..3531
                     /locus_tag="PLE93_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85806.1"
                     /translation="MGERRRLILAFVLGLVIATAGTATAAKLITGKQIKDGTITSKDL
                     SRALRKQISRSGKAGPTGPAGVTGPAGVTGPTGAVDASGFFTKAESDARYPQIDVVQS
                     SGEYAVSVPSGTMQAIATLLCPDNSLAAKVGYEATGFDQGLVSAQLDASTVDIVINNT
                     GPNPANGTLDVKLNCFQIDPAIVPDPTFP"
     gene            complement(3533..>4385)
                     /locus_tag="PLE93_01780"
     CDS             complement(3533..>4385)
                     /locus_tag="PLE93_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH85807.1"
                     /translation="KGARKPVPPKECPACGTEVIKPEDSVFSICPNRSGCPGQIFQHI
                     KHFVSRGAMDIEGLGEKQVARFLEEGLISDVADIYALRQEQLEALDRMGETSAGNLIA
                     EIEKSREIPFPRVLYALGLIGVGSVTAEALADEFGSIDALHEADPERIEQTEGVGPVI
                     ARQIAESLADEGTWALVERLKAAGLKMELAEDERRPEGGPLEGKTVVLTGTLPDLTRD
                     EAGAMVKRAGGKVTGSVSKKTDFVVAGESAGSKLEKAEKLGVTVLDREGLDQLIAGNL
                     PPEPEGD"
BASE COUNT          736 a         1472 c         1371 g          806 t
ORIGIN      
        1 aggatccggc ggccgtcgtc cgagagtcgg acatcgacga tgcccttggt cgcgagcgcc
       61 tcgcgcacat aggcgccgat gcccgcgttg gagtacatgc cggaaggcag gtgctcggcc
      121 aggccgtagg caatgtcgat caccgacagg tcacggaagc gcgggaccat gctcttgacc
      181 atgccggcga catgcagcgg cagggtgacg atccgctccg cgtattcgct gaaattcggg
      241 cgaaggacgg tgtcctggct gatccgttcg agcagggcgc ccttttcatc ggtcaggacc
      301 cgcatcattt cctccggggt gaccccggcc gcgagcatgc tgccgatgaa cgagccggcg
      361 cttgtgccga tgtacatgtc gaagtcattg accgtcgaat taacggcaag gagatcaagc
      421 gcccggaggg ttccgatctc gtatacgccg ccggtgaaac caccgccacc gaggaccaac
      481 gcagtgcggg aacgcttctg ccttttggcg ctctggcgcc gtttctggga ttcctcgatg
      541 tttacgacct tgcccatgag ggtccaatta tgacagcaat cggtgtcacc ttccgctacc
      601 ttcggcccca agtttgttgc aatatccgat gaaacactct cttccgctcc tcgctgcggt
      661 gctttccctg gccctcgcag ctccttccgc ggccgcttcc cccgacccgc tgccgaatcc
      721 ggaccggcat ttcgccacac cgttcatgca ccggggtgaa aagggcgcgt ccggcccggc
      781 cgatgcgagc gccagcgcgg gccgggcctg ccaggtgatg agaatgctgg ccaggcgagg
      841 caatggtgcg gtcggcctgc gggtgttcaa ccttcgcagc aggaagaacg tctgcgccct
      901 gaactcgggg gcccgccgtt cgctggcctc gaacaacaag ctcttcacga tctccaccgc
      961 cctcgaccgg ctcggccagg atcagcggct tcaaacccgg gtgatggccg gcggcaagac
     1021 aagccggaac ggggttctcg acggcaacct ctacctgaag ggcggcggcg accccagcct
     1081 cggaaccgcc aggttcgtga accgctggtt cggtggtcac ggatcggaaa tcgagaagct
     1141 ggcccggaag gtccgccagg cggggatccg caaggtcacc ggccggctct tcttcgacga
     1201 ttcggtcttc gaccaggtcc gtggcgtcgc cgattccggt tatgcgacca gcccctggat
     1261 cgggccgctc tccggaatcg ccttcaacga gggctacacg agcccttcgc tttcccggtt
     1321 cagcaacgat ccggccaggc tggcggcccg gactttcgtt cgggcgatgc gggcccgcgg
     1381 agtgaagatc cgcccgcgat tctcaccccg ggacacaccg gtgaagctgc tcaagcgggt
     1441 agtcgcccgc gaggtttccc ccgacatggg ctggatggcc cgggccaccc tgctggactc
     1501 gaacaacttc ttcgcggaaa ccctgctcaa ggtgctcggg gcctccttcc acggcctggg
     1561 aacgaccgcc aacggggtca aggtggtgcg ccggaccatg gcttcgctga aggtggatgt
     1621 ctggccgatc gacggctcgg gcctcaccta cgggaaccgg tccaccgctc acgacgtggt
     1681 caagctgctc ttccgcgccc gcagcgaacc ctggggcaag gccctcgtcg ccggcatgcc
     1741 gaccgccggg gtggacggca ccctcgagga ccggatgcgc ggcagcgctg cccagggcaa
     1801 ctgccatgcg aagaccggca ccctgaccgg cgtctcggcc ctctccggat actgcttcaa
     1861 cagctccggc cggcgattcg ccttctcgat cctgatgaac gacgtgacca acctggacac
     1921 cgcccaccgg gcccaggacc gcatcgccgc cctgatcgcc cggctttagt ggcgggttgg
     1981 cgggttggat cccgccctac tccttatttc ctgagccatt gctcgacttc ggcgtagtcg
     2041 ccggtcgcca cgagataccg gatcttgccg gttttgaggg tgaggatgcc gatcttcttg
     2101 tttccttcga ccccctgggt gaaggcgatc atcttgccgt cgggcgaata gtgaggatcg
     2161 aagttgatcg ccccggtccg ggtcagtcgc ctggcgttct tgccgtcaag gcgtgactgg
     2221 accagctccc aggcgtcccc gtactgcgcg gtgtagacca tcgacttgcc atcgggggag
     2281 aacgccggcc ccagcgccgc gcctttcgtg ttgaccgtgt ggctcttgcc ggtgcgcgtg
     2341 tccagcagcc tgatcttcga ggcgtcctcg tcgccgcccc ggttgtaggc gaggtaacgg
     2401 ccattgggcg aaacgtcgga gaagccccag tccagtttgg tgtcgacttt gtaggacgtc
     2461 gtcttcttcg tcttgaaggt gtacttcttc aggtgccaga agacccccga atcgaggtag
     2521 tacttcttcg agaagtagat cgacttgccg tccggcgcga atacggtttc gaggttcagc
     2581 acgtcacttt cctctggggt gatcgccttc gcctgcttcg gcttggcgaa ggagccgagc
     2641 cacatccggt agccgggata gaccgagtag acgatctgct tgccgttcgg tgagaccgcc
     2701 ggatcggaat acgactggtc gagggcgcca agcagttcct tcgactttcc ggtcagggtc
     2761 tcggtgaaga tgcggctcgt gctcccgttg atgccaccat cggtgtagcc ggaaacgagc
     2821 agcagcccgt tcttgcccgg caccgtcgcc gaggcgctgc caaccgcgat cgtgaagacc
     2881 gcgacccccg ccacggccag aaaccttctg gcactccctg aaaaactcat gccgcccagc
     2941 ctaatcggtt atgaatgaca catgggagaa cggagaaggc tgatactcgc ttttgtcctc
     3001 ggcctggtta tcgccaccgc cgggacggcg actgcggcaa agctgatcac cggaaagcag
     3061 atcaaggacg gcacgatcac ctcgaaggat ctgtccaggg cgcttcgcaa gcagatcagc
     3121 agatccggca aggccggccc gaccggtccg gccggcgtga ccggaccggc gggtgtaacc
     3181 gggcccaccg gggcggtcga cgcctccggt ttcttcacga aagcggaaag cgacgcccgc
     3241 tacccgcaga tcgatgtggt ccagtcgagc ggggagtacg cggtttcggt tccttccggg
     3301 acgatgcagg ccatcgcgac actgctctgc ccggacaatt ccctcgcggc gaaggtgggc
     3361 tatgaggcca ccggcttcga tcagggcctg gtttccgccc agctcgacgc cagcaccgtc
     3421 gacatcgtca taaacaacac cggacccaac ccggcaaacg gaactctcga cgtcaagctc
     3481 aactgcttcc agatcgaccc ggcgatagtg ccggatccga cctttccctg aatcagtccc
     3541 cttcgggctc cggagggagg ttcccggcga ttagctggtc aaggccctcg cggtcgagaa
     3601 cggtcacccc gagcttctcg gccttttcga gtttggaccc ggctgactct ccggccacca
     3661 cgaagtcggt cttcttcgag accgaaccgg tgaccttgcc accggcccgc ttgaccatcg
     3721 ccccggcttc atcacgggtc aggtcgggca gggtgccggt caggacgacg gtcttgcctt
     3781 ccagcgggcc gccctcggga cgacgctcat cctcggccag ttccatcttc agaccagccg
     3841 ccttgaggcg ctcgaccaga gcccaggtcc cttcgtcggc cagtgactcg gcgatctgcc
     3901 gggcgatgac cggaccgacc ccctcggtct gctcgatccg ctccgggtcg gcctcatgca
     3961 acgcgtcgat cgaaccgaac tcgtcggcca gggcttcggc cgtaaccgat ccaacgccga
     4021 tcagaccgag cgcgtagagg acccggggga aggggatctc ccgggacttc tcgatctcgg
     4081 cgatcaggtt gccggcggag gtctccccca tccggtccag cgcctcgagc tgctcctgcc
     4141 tcagggcgta gatgtcagcg acgtcgctga tcaggccttc ctcgaggaaa cgggcgacct
     4201 gcttttcacc gaggccctcg atgtccatcg ctccgcggga gacgaagtgc ttgatgtgct
     4261 ggaagatctg gccggggcag ccggaacggt tcgggcagat cgagaagacc gaatcctccg
     4321 gcttgatcac ttccgtcccg caggccgggc actccttcgg cgggaccggc ttcctcgccc
     4381 ccttc
//
LOCUS       DAOQFI010000058         4295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8896_length_4295_cov_3.635142, whole genome shotgun sequence.
ACCESSION   DAOQFI010000058 DAOQFI010000000
VERSION     DAOQFI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8896_length_4295_cov_3.635142"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLE93_01785"
     CDS             complement(<1..694)
                     /locus_tag="PLE93_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85808.1"
                     /translation="MPINGRSLRSTVTSDGALELRLIEEEIEEPTGSQVLVSVEATPV
                     NPSDLGVLIGPADASTLGPAEGGGLNGKVPEQALPIMRDRLDKPLPVGNEGAGTVVAA
                     GPEAADLVGRTVSLVGGAMWSDYRLVDGAAVIPLPDDATPADGAALFVNPMTALSMVE
                     TMRAEGHTALVHTAAASNLGQMLVKICKDDEVGLVNIVRKPEQAATLEALGAEHVVDS
                     SLPDFDQRLVEAI"
     gene            complement(758..1153)
                     /locus_tag="PLE93_01790"
     CDS             complement(758..1153)
                     /locus_tag="PLE93_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH85809.1"
                     /translation="MLSLHHTHLMASDIDETIRFWAEGFGGEVVFDREFAGARNVFMT
                     VGKGRIHLYDQAPKVSGQAVVHHLGVQTDEIETVVARLRSMDISVTDIRTEPDASYAM
                     AGGPDDLLVEIFQPDAEAVPEELRPYFGL"
     gene            1244..1597
                     /locus_tag="PLE93_01795"
     CDS             1244..1597
                     /locus_tag="PLE93_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH85810.1"
                     /translation="MSPELTLTPKQKSPNGQPCCEPVVYPDVDRARAERMAKVAKALG
                     DPIRLTLVDVLRKHAGKVCVCELVPLFDVNQSTLSHHLKKLRDAGIVDSERQGLWSYY
                     YVNPEELEELSAWLS"
     gene            1585..2463
                     /gene="arsM"
                     /locus_tag="PLE93_01800"
     CDS             1585..2463
                     /gene="arsM"
                     /locus_tag="PLE93_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNH85811.1"
                     /translation="MAELEKTTDCCSPEDQATCCEPEAKSDCCGVGPECGCPDGAERT
                     DSELREIVRERYAAAARDAGSRDASCCGNDAVITEDQRAVFGSSLYEDHDRSELPEAA
                     QLASLGCGNPTAIADLQEGQTVLDLGSGGGIDVLLSARRVGPAGKAFGLDMTDEMLEL
                     ARSNQAEAGVENVEFLKGEIEAIPLPDDSVDVVISNCVINLSGDKPRVLREAARVLRP
                     GGRFAVSDVIADPDMSEETREDMKQWTGCIAGALTRDEFESNLLAAGFENVEIEETHR
                     VHDQAWSAIVRATMPS"
     gene            2460..3308
                     /locus_tag="PLE93_01805"
     CDS             2460..3308
                     /locus_tag="PLE93_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNH85812.1"
                     /translation="MTGDEIQLNDPQTLYERWEGAQWNPFTVPLGLDQEQWSGLGETD
                     RGLIYWVLSSLMVAEERITTKFSGLVGAHGSEEEASFLATQQVDEARHMQFYARFQNE
                     VIADPATVAAHVDRSREQVSPAFEQIFDVELVQAHEHLIANPGDLAAKVQFVTLYHLI
                     LESTLGLTTFKFVTDYLKEHELLPGFVDGYSKIHHDETRHIGYGVWFLRESVRESPEV
                     APAAVREMLRKLLPSVAESLSPSSEPGGPDIEALGVDDEQIREFALGGLTRRLEIIGV
                     PLESVF"
     gene            complement(3297..4109)
                     /locus_tag="PLE93_01810"
     CDS             complement(3297..4109)
                     /locus_tag="PLE93_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNH85813.1"
                     /translation="MAGAQLKLVTWNVAGRTRLLTQQAAALSRTEPDLVTLQEIRKGT
                     VERWKAALAEDGLVHTIDSGYRTAERRLFNLTASRWKLSDLGDAPVPQPERVVSAVLD
                     TPFGPCELHNVHVPPAPSQGLAKLETLERLHDWLAVPGSHHRILCGDLNVPRLEYPDG
                     VIETFASNHPPEQFERWDRAEKLLLTGLAEYGLKDVFRSLHGYESPEPSWFPHRRNPG
                     PGLPGHRLDHVLASDSLNAVWAGYQHGWREAGLSDHSALEVIFEPRAGNLEN"
     gene            complement(4109..4240)
                     /locus_tag="PLE93_01815"
     CDS             complement(4109..4240)
                     /locus_tag="PLE93_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85814.1"
                     /translation="MNWRACIDRNTEGVRHVEVGSSHLGLGLDPDVWLATAEALAAA"
BASE COUNT          776 a         1330 c         1433 g          756 t
ORIGIN      
        1 aaatggcttc gaccagccgt tggtcaaagt cgggcaggga ggagtcgacc acatgttcgg
       61 cgcccagcgc ctcgagtgtg gcggcctgct cgggcttgcg cacgatgttg accagtccga
      121 cctcgtcgtc cttgcagatc ttgaccagca tctggccgag gttcgaggca gcggcagtgt
      181 gtacgagcgc ggtgtggccc tcggcccgca tcgtctcgac catggaaagc gcggtcatcg
      241 ggttcacgaa gagggcggcc ccgtcggcgg gggtggcatc gtcgggcagg gggatgaccg
      301 cggccccgtc gaccagccgg taatcggacc acatcgctcc gccgacgagg gagaccgtcc
      361 gtcccacgag atcggccgcc tcgggcccgg cggcgacgac cgtgccggcg ccttcgttgc
      421 cgaccggcaa cggtttgtcg agccggtcgc gcatgatcgg cagggcctgt tccggcacct
      481 tgccgttcag gccgccgcct tcggccgggc cgagcgtcga ggcatcggcc ggaccgatca
      541 gaacacccag gtcggaaggg ttcaccggcg tcgcctcgac cgagaccaac acctgggacc
      601 cggtcggctc ttcgatctcc tcctcgatca accggagctc gagggctccg tcagaggtga
      661 ctgtcgaccg caaactccgt ccgttgatgg gcatggacgc cattgtgacc aatttgacct
      721 cccccgtcca cgaatggccg acgggagccc aaggcgttta caggccgaag taggggcgga
      781 gttcttcggg gaccgcttcc gcgtcgggct ggaatatctc caccagcaag tcgtccggac
      841 ccccggccat tgcgtatgag gcgtcgggct cggtccggat gtcggttacc gagatgtcca
      901 tgctgcggag tctggccaca acggtttcga tttcgtcggt ctggacgcca aggtgatgga
      961 ccaccgcctg gccggaaacc ttcggcgcct ggtcataaag gtggatccgg cccttgccga
     1021 cagtcatgaa gacgttcctg gcgccggcga actcgcggtc gaagacgacc tcgcccccga
     1081 atccttcggc ccagaagcgg atcgtctcgt cgatgtcgga ggccatcagg tgggtgtggt
     1141 ggagggagag catcaggtga gttaagacta gacgcgtctt ctttccatcg aagttcatcg
     1201 gctctattga caaatatcaa tggaactgat ctaatgcgct ccgatgagcc ccgaactaac
     1261 cctgaccccg aagcagaaga gcccgaacgg ccagccatgt tgcgagccgg tcgtctatcc
     1321 ggatgttgac cgggcccggg cggaacggat ggcgaaggtc gcgaaggcgc tgggggaccc
     1381 gatccggttg accctggtcg acgtccttcg caagcacgcc ggcaaggtct gcgtctgcga
     1441 actcgtaccg ttgtttgatg tcaaccagtc gacgctttcc catcacttga agaagctcag
     1501 ggacgccggc atcgttgatt cggaacgtca gggcctgtgg tcctactact acgtcaaccc
     1561 cgaggaacta gaggaactga gcgcatggct gagctagaga agaccaccga ctgctgttcg
     1621 cctgaagacc aggccacctg ctgcgaaccc gaagccaaga gcgactgttg cggcgtgggg
     1681 ccggagtgcg ggtgcccgga cggtgccgaa agaaccgatt cggaactgcg ggagattgtc
     1741 cgtgagcgat atgccgccgc ggccagggac gcaggttcca gggacgcatc ctgctgcggc
     1801 aacgatgccg tgattaccga ggaccagcgc gcggtgttcg gttcgagcct ttacgaggat
     1861 catgaccgca gcgagctgcc cgaggccgca cagctggcgt cactcggctg cggaaacccg
     1921 accgccatcg ccgatctcca ggagggccag acggtgctgg atctcggatc aggcggcggc
     1981 atagacgtcc ttctctcggc caggagggtg ggcccggccg gcaaggcttt cgggctcgac
     2041 atgaccgacg agatgctgga gctggcaaga agcaaccagg ccgaagcggg cgtcgagaac
     2101 gtcgagttcc tgaaggggga gatcgaagcg attccccttc ctgacgattc ggttgacgtg
     2161 gtgatctcca attgcgtaat caacctctcc ggtgacaagc cccgggttct gcgcgaagcg
     2221 gcaagggtcc ttcgtccggg aggccggttc gcggtctcgg acgtaatcgc cgatccggat
     2281 atgtccgagg aaacccgcga ggacatgaag caatggaccg gctgcatcgc cggggccctg
     2341 acccgggacg agttcgagtc caacctgctg gcggcgggct tcgagaacgt cgagatcgaa
     2401 gagacgcacc gggtccacga ccaggcatgg tcggcgatcg tgcgtgcgac gatgccatcg
     2461 tgaccggcga cgagattcaa ctcaacgacc cccagaccct ctacgaacgc tgggagggtg
     2521 cgcaatggaa tcccttcacc gtcccgctcg ggcttgacca ggaacagtgg tccggactgg
     2581 gtgagaccga tcggggactg atctactggg tgctgtcttc cctgatggtc gccgaggaac
     2641 gaatcaccac gaagttctcc ggcctggtcg gcgcccacgg ctccgaggag gaggcgagct
     2701 tcctcgccac ccagcaggtt gacgaggccc gacacatgca gttctatgcc cggttccaga
     2761 acgaggtcat cgcggatccc gcgacagtgg cagcacatgt ggatcgttcc cgggagcagg
     2821 tctcacccgc cttcgaacag atcttcgacg tcgaactggt ccaggcccat gagcatctga
     2881 tcgccaaccc cggcgatctg gccgcgaagg tgcagttcgt gacgctctat cacctgattc
     2941 tggagagcac gctcggtctg accactttca agttcgtgac cgactacctg aaggagcacg
     3001 aactgctccc tggtttcgtc gacggatact cgaagatcca ccacgacgaa acccggcaca
     3061 tcgggtatgg ggtctggttc cttcgcgagt cggttcggga gtcgcctgaa gtcgcaccag
     3121 ccgccgtccg ggagatgctg aggaagctgc ttccgtcggt cgccgagtcg ctctcgccgt
     3181 cctcggaacc cggcggcccg gacatcgagg cactgggggt cgacgacgag cagatcaggg
     3241 agttcgcatt gggcgggctg accaggcgcc ttgaaatcat cggcgtaccg ctcgaatcag
     3301 ttttctaggt tccccgcacg gggctcgaag atcacttcca gggccgaatg gtcgctaagg
     3361 ccagcctccc gccaaccatg ctggtacccg gcccagacgg cgttcagaga gtctgacgcc
     3421 aggacgtgat cgagccggtg gccgggcagt cccggtcccg ggtttcgccg gtgggggaac
     3481 cagctgggct cgggtgattc gtagccgtgc aggcttcgaa acacatcctt cagtccgtat
     3541 tcggcgagcc cggtcaacag aagcttctcg gcccggtccc agcgctcgaa ttgttcggga
     3601 gggtggttac tggcgaaggt ctcgatcact ccgtccggat attccagccg gggaacgttc
     3661 aggtctccgc agaggattct gtgatgggaa cccggcaccg ccagccagtc gtgaagccgc
     3721 tcgagcgtct ccagcttcgc gagaccctgg ctgggagcgg gagggacgtg aacgttgtga
     3781 agctcgcagg gcccgaaggg tgtgtccagg acggccgaga cgacgcgctc gggctggggg
     3841 acgggcgcgt ctccgaggtc gctcaacttc caccgcgagg cggtcaggtt gaatagtcgc
     3901 cgttccgcgg tccggtagcc ggagtcgatg gtgtggacaa gtccgtcttc ggccagggcc
     3961 gccttccatc gctcgacggt gccttttcgt atctcctgca gcgtgaccag gtcaggttcg
     4021 gtccggctga gtgcggcggc ctgttgcgtt agaaggcgag tgcgaccggc cacgttccag
     4081 gtgaccagtt tgagctgtgc accggccatc aggccgcagc gagggcctcg gcggtggcca
     4141 gccagacgtc gggatccagt cccagaccga gatggctcga gccgacttcg acgtgacgga
     4201 caccctcggt gtttcggtcg atgcaagcgc gccagttcac gaccccgtcg cgccgggcga
     4261 tcgggagccg cgtgcgaact caggaaatgg aggcc
//
LOCUS       DAOQFI010000059         4267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_8999_length_4267_cov_3.556743, whole genome shotgun sequence.
ACCESSION   DAOQFI010000059 DAOQFI010000000
VERSION     DAOQFI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8999_length_4267_cov_3.556743"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1531)
                     /gene="pyk"
                     /locus_tag="PLE93_01820"
     CDS             complement(95..1531)
                     /gene="pyk"
                     /locus_tag="PLE93_01820"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNH85815.1"
                     /translation="MNQIHSETRRTKIVATVGPASWDPSVLEQMIIAGVDVFRLNFSH
                     GDEATHVRVIKDVRLISERLDKEVGILGDLPGPKLRVGEYPQGVVDIIHGSTVTITPD
                     DVPGTETLIPVDWPELAQALSVEDTVYLADGSIRLRVVANDGRTVECEVEVGGPLSSH
                     KGMNLPGVEAGLDSVSEDDLRWVEFAVANQLDYIAVSFVRKAEDLDPVLNKLKELRSD
                     IPIIAKIEKPQAADAVEEIVDRATGGIMVARGDLGIEVPLSKVPVLQKSLIGAAGRAS
                     KTVITATQMLASMVSAKRPTRAEVTDVATAIYDGTDAIMLSEETAVGEFPVEAVRVMD
                     QIARGTETDLPYWDWVLNRVPQDDQDVSDSVTQSAVIAAYRLNLQAIVVPTASGRTAK
                     VVAAHRPQVPVLAVTHSVRTQRQLKLIFGVRPVVNDQTTEIRNLLYECADDAVRFGAA
                     ASGDLIAIVAGLSDMQLGTNLFEVHRVP"
     gene            complement(1811..>4267)
                     /locus_tag="PLE93_01825"
     CDS             complement(1811..>4267)
                     /locus_tag="PLE93_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNH85816.1"
                     /translation="LLSFLGLAVPVANAAPKDYSAIARNIIPSGQKGTPPLDTTQAVM
                     YDALTPLFNNVTDSDIKEYFKSEKLGTSTDGPYTTETVPKAGVTIQRDKYGVPHVTAT
                     TYDGGIWAAGWIAAEDRGLLLQQARYNARVAAIDAPGLSAIGLVAGLQNFQPSQRTED
                     EIAKQTDALKAAGSEGKAVLKDIDTYLTGINDYLDANSPATPDWTRNDVYAVNALKDQ
                     FLGEGGGDEARRSQFLGGLIKRLGAKKGWKVFNDLRQHTNNESPTSIDGRFAYSPIPS
                     NKSGSVIIDPKSFKPVDVTPNTPEELSDRSSSNPLEPRHQASNTLMITKGRSATGRPL
                     MVGGPQIGYFAPGLTYEIDMNAPGLKWRGATSAPFPGYLLIGRGQDFATTLTSASGDV
                     IDQFAETLCGGSDTKYLYKGKCRTMGTFDAGTLNGDPVSFRTTVHGPVVGYATVKGKK
                     VAISSKRSSYGKDVLDLMFNRRLSNGSVKGPQSFFEAASKTPQTFNSFYIDNKNVALY
                     TSGKLPIRDKRVDPGLLTKGTGQYEWKGFLSKKGHPHGMNPKSGMMVNWNNSVARGFG
                     AADDEWGRNGSVQRVGLLTRMLNRNKSKNGKLNMADVVSAMNAGATQDVRAIFTVPLL
                     AKLLKGSKAPSTQARQMLNQMVDWNKKNGSRLDRDGDNLIDAPGAASLDAAWPKIADA
                     FMKPVIGSQLDELSSLFSRFDQPPSGQYSGWYQYFDKDIRALLGQKVKSPFKVKYCGH
                     GKKAACQKAVWNAIAAAGTEAAAQYGSENPAQWHVNTAYVDDDHRGEQIKFSPLTLVT
                     MRYTNRPSGIQQVISFNGHR"
BASE COUNT          672 a         1336 c         1440 g          819 t
ORIGIN      
        1 cacctcgatc gctggcatcg aggttcagcc tacagaaccc ggttcaagtc accgaattga
       61 ttacccgccc gccccgcgcg gagcccgcgg ccgctcaggg cacgcgatgc acctcgaaca
      121 ggttggtacc cagctgcatg tcggatagtc cggccacgat cgcgatcagg tcacccgacg
      181 ccgccgcccc gaaccggacc gcgtcgtcgg cgcattcgta gagcaggttg cggatctcgg
      241 tggtctggtc gttgacgacc ggccgcacgc cgaagatcag cttgagttgc cgctgggtgc
      301 gcacgctgtg ggtcaccgcc agcaccggaa cctgcgggcg gtgggccgcc acgaccttgg
      361 cggtgcgacc ggaagcggtc ggcaccacga tcgcctgcag gttcagccgg taggcggcga
      421 tcaccgccga ctgggtgacc gagtcggaga catcctgatc atcctgcgga acccggttga
      481 ggacccagtc ccagtacggc aggtccgttt cggtgccgcg ggcgatctgg tccatgaccc
      541 tgaccgcctc gaccggaaac tcgccgaccg cggtctcttc cgagagcatg atcgcgtcgg
      601 tgccgtcata gatcgcggtc gcgacgtcgg tcacctcggc cctggtcggg cgtttggccg
      661 aaaccatcga ggccagcatc tgggtcgcgg tgatcaccgt cttggaagcc cggccggctg
      721 ccccgatcag gctcttctgc agcacgggaa ccttggaaag cggcacctcg atgccgaggt
      781 cgccgcgggc aaccatgatc ccgccggtgg cccggtcaac gatttcctcg accgcgtcgg
      841 cggcctgcgg cttctcgatc ttggcgatga tcgggatgtc cgagcggagc tccttcagct
      901 tgttcagcac ggggtcgaga tcctccgcct tgcggacgaa ggaaaccgcg atgtagtcga
      961 gctggttggc gaccgcgaac tcgacccagc gcaggtcgtc ctcggaaacc gagtcgagcc
     1021 cggcttcgac gccgggcagg ttcatgccct tgtgcgacga gagcggcccg ccgacttcta
     1081 cctcgcactc aaccgtccgc ccgtcgttgg caaccacgcg caggcggatc gagccgtcgg
     1141 ccagatagac cgtgtcctcg acactcaggg cctgggccag ttcgggccag tcgaccggga
     1201 tcagggtttc ggttccaggc acgtcatcgg gcgtgatcgt cacggtcgaa ccgtggatga
     1261 tgtccaccac tccctgcggg tattcaccga cccggagctt tggccccggc aggtccccga
     1321 ggatcccgac ttccttgtcg agccgttccg agatcaggcg aacgtccttg attacccgta
     1381 cgtgagtcgc ctcgtcaccg tgagaaaagt tgagccggaa gacgtctacc ccggcaatga
     1441 tcatctgctc gagcacggac ggatcccatg aggcgggacc gaccgtggcg acgatcttcg
     1501 tacgacgggt ttcggagtgg atctggttca cccccggaat actctcagtc cggcgggtcc
     1561 ggcctgtaaa cgcctgtaaa cgggggtccc gctcgcgcgg actgatcaac ctatccgcag
     1621 ttgaacggcg gacttcggtt ttcagttcgg aatccggggt aaaccgaaat tacggccttc
     1681 attccaccgg ggcgatcccc ctacgacccg atggataggc ctggcggtga gtgcctgcac
     1741 cgccatggaa agaggaaagc ccgccccagg gatggggcgg gctttctcct cgatcgggct
     1801 ttggccggac ctagcggtga ccgttgaagg agatcacctg ctggatgccg gacggcctgt
     1861 tggtgtagcg catggtgacc agggtgagcg ggctgaactt gatctgctcg cctcgatggt
     1921 cgtcatccac gtaggccgtg ttcacgtgcc actgggccgg gttctctgag ccgtactggg
     1981 cggccgcttc ggtgccggcg gcggcgatcg cattccagac cgccttctgg caggcggcct
     2041 tcttgccgtg gccgcagtac ttgaccttga acggactctt gaccttctgg ccgagcagcg
     2101 cccggatgtc cttgtcgaag tactggtacc agccggagta ctggccactc ggcggctgat
     2161 cgaaccgtga gaacagcgag ctcagctcgt cgagctgcga cccgattacc ggcttcatga
     2221 acgcgtcggc gatcttcggc caggcggcgt ccagcgaggc ggcgcccggg gcgtcgatca
     2281 ggttgtcgcc gtcacggtcc agacggctgc cgttcttctt gttccaatcg accatctggt
     2341 tgagcatctg ccgggcctgg gtactggggg ccttcgagcc cttcagcagc ttggccagca
     2401 gcggcacggt gaagatggcg cgcacgtcct gggtcgcacc ggcgttcatc gccgaaacga
     2461 cgtcagccat gttcagcttg ccgttcttgc tcttgttccg gttcagcatc cgggtcagca
     2521 ggccaacgcg ctggaccgaa ccgttgcggc cccactcgtc atcggcggcg ccgaagccac
     2581 gggcgacgct gttgttccag ttgaccatca ttcccgactt cggattcatg ccgtgcgggt
     2641 ggcccttctt ggagaggaag cccttccact cgtactggcc ggtgcccttg gtcaagaggc
     2701 ccgggtcaac ccgcttgtcg cggatcggca gcttgccgct ggtgtagagc gcgacgttct
     2761 tgttgtcgat gtagaacgag ttgaaggtct gcggggtctt cgaggcagcc tcgaagaatg
     2821 actgcggacc cttgaccgaa ccgttcgaca ggcgacggtt gaacatcagg tcgagcacgt
     2881 ccttgccgta gctggagcgc ttcgaggaga tcgcgacctt cttgcccttg accgtggcgt
     2941 agccgacgac cggaccgtgg acggttgtcc tgaaggaaac cgggtccccg ttcagggtgc
     3001 cggcgtcgaa agtgcccatg gtgcggcact tgcccttgta caggtacttg gtatcgctgc
     3061 cgccacagag cgtctcggcg aactggtcga tcacgtcgcc gctggccgag gtcagcgtcg
     3121 tcgcgaagtc ctggccgcgg ccgatcagca ggtagccggg gaacggggcc gaggtggcac
     3181 cgcgccactt cagaccgggc gcgttcatgt cgatctcata ggtgagaccg ggggcgaagt
     3241 agccgatctg cggtccgccg accatcagag gacggccggt ggccgaacgg cccttggtga
     3301 tcatcagcgt gttcgaggcc tggtgacggg gctcgagcgg gttcgaactg ctccggtcgc
     3361 tgagctcctc gggagtgttc ggggtcacgt cgaccggctt gaagctcttc gggtcgatga
     3421 tgacgctgcc ggacttgttc gaggggatcg gcgagtaggc gaaacggccg tcgatcgagg
     3481 tcgggctctc gttgttggtg tgctggcgca ggtcgttgaa gaccttccag cccttcttcg
     3541 caccgaggcg cttgatcaga ccgccgagga actgcgaacg gcgggcttcg tcgccgccgc
     3601 cctcaccgag gaactggtcc ttcagcgcgt tgaccgcgta gacgtcgttg cgggtccagt
     3661 ccggggtggc cgggctgttc gcatcgaggt aatcgttgat gccggtcagg taggtgtcga
     3721 tgtccttgag gactgccttg ccttcggagc cggcggcctt gagggcgtcg gtctgcttgg
     3781 cgatctcgtc ttcggtccgc tggctgggct ggaagttctg cagcccggca accaggccga
     3841 tcgcgctgag gcccggcgcg tcgatggcgg cgacccgggc gttgtaacgg gcctgctgaa
     3901 gcagcaggcc gcggtcttcc gcggcgatcc agccggccgc ccagatgcca ccgtcatagg
     3961 tggtggcggt cacgtgcggc acgccgtact tgtcgcgctg aatcgtgacg ccggccttcg
     4021 gcacggtctc cgtggtgtag ggaccatcgg tcgaggtgcc aagcttctcc gacttgaagt
     4081 actcctttat gtcactgtct gtgacgttgt tgaagagcgg ggtcagcgcg tcgtacatca
     4141 cggcctgggt ggtgtccagg ggcggagtgc ccttctggcc ggaggggatg atgttgcgcg
     4201 cgatcgcgga gtaatccttg ggagcggcgt tcgcaacggg aacggcgaga ccgaggaaac
     4261 tcaagag
//
LOCUS       DAOQFI010000060         4263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_9004_length_4263_cov_3.208175, whole genome shotgun sequence.
ACCESSION   DAOQFI010000060 DAOQFI010000000
VERSION     DAOQFI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9004_length_4263_cov_3.208175"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLE93_01830"
     CDS             complement(<1..853)
                     /locus_tag="PLE93_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85817.1"
                     /translation="MPAAILAFVAFLLLLSAVETPAEAALPEVLSGRTIVQPSPLEKG
                     PGSLDYSHSGWRVSEGGKGYDAWIAFEPTGPRPARAPLAIVTHGYGEFSGYDRLYELI
                     RHTVLKGNVVIFPRWQISDFDPCPGPFNIKACMDSAVTGIHAALEYLRESPNRVQPDT
                     GRTSYYGYSFGGIITANIANQYRFYGLPRPRAIFLDDPHDGEVAGLGEPALDDSLAGI
                     PSDVKFECHSGADGVLGVPRLIEGSCNAVFPKLTHIPARNKALVMTRTDSWGTPVLSS
                     AHGVCASN"
     gene            complement(938..1615)
                     /locus_tag="PLE93_01835"
     CDS             complement(938..1615)
                     /locus_tag="PLE93_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH85818.1"
                     /translation="MAVQSPHDLIPRVREGVFFEPLQALPRGRHSLSGEEVRAIQQER
                     ILVSMTELMAAHGAPGIGSNEIARHAGVSLAAFYECFDGKEACALAGYDRFIEVLFHA
                     MNAVDVGSRTRPELVCGLIRAYLETLQSDLVVARAYQVEIDSLGASVRKRRRDSLLLF
                     AHHLWGVSSDDSPTGRGPEQLPWSAYIGIIYTTRQLASDALDTEEKPDLIALSDELAI
                     WVTDLFR"
     gene            1768..3756
                     /locus_tag="PLE93_01840"
     CDS             1768..3756
                     /locus_tag="PLE93_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85819.1"
                     /translation="MSKQGEGKGEEDPGVREVTPAEAGGITRRGFLARSAATAATAAT
                     VGSLTGAGGASAAGSESRKVPGSFGEVALPDARQVKADYQRMVDFGPRLPGYEEHLEF
                     CNWIEDEFVKAGLELLPTDQIPYRRWRPGKMSLRVGTTDFDPATTYVRVPGTPAEGIT
                     APMMLAGDGPVDGKIVLVEAAVPTPRTAGLFIAMADYWQWRGHTAFEAAAEEFDRFAF
                     SDWPTIKPYADQGAKAVIFISRASRAEVEGNWSPHQAEVQPIPGFVVDRDMGAELKAL
                     AAGQPEATLKLEADWKDTYVPQITGILPGESDEVLIVNSHTDGQNAFEENGAVALVHL
                     ARHFASLRGSRKLKRTLVFIAWPGHMANVSNRPDDRTTLPEQFGWISSHRDLFDRTVA
                     AVTIEHLGATRWSDDPVEGYHSTGMSDFYAIWTTLGAMQDLCKVALKESDLQRHALLR
                     PPIQVTPGVAWHHRGIPHVAGISAPKYLVRVSENGDIDKLDASLASRQTGFYADVVKS
                     LDSADPAALKAGGEGDSSFNSVSDREYPMTRARSGPAREVVLDAGDGGRLAIYIGGRA
                     AGGRDIGVQMAALDHSLERVTVAVYRDDTLLGKSRPTRVDRKVKRVAIHRSGHRADHR
                     NLHRNRSAARFRDGTYTFVVRQDGKLLGQRNVTYQRRR"
     gene            3823..>4263
                     /locus_tag="PLE93_01845"
     CDS             3823..>4263
                     /locus_tag="PLE93_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85820.1"
                     /translation="MKETTIQDLGGTGRARVFLLAAFVALAGMLGMAGSSKAETHTIT
                     VDNGRLTMGFVFLNNKILPADVPGPLDPNISNTSGSGTIAVETSGSTATVAEADFNMP
                     IVWIPDPTAGGAPIPMKFGPKGDLTGTWDAGTGQLSLTGTLQVDV"
BASE COUNT          770 a         1367 c         1447 g          679 t
ORIGIN      
        1 gattggaagc gcagaccccg tgggccgaac tcagaaccgg agttccccat gagtcggttc
       61 tggtcatgac cagggccttg ttgcgggccg ggatgtgggt gagcttcggg aagaccgcgt
      121 tgcaactgcc ctcaatcagg cgagggacgc cgagcacgcc gtcggcgccc gagtggcact
      181 cgaacttgac gtcggagggg atcccggcga gggagtcgtc gagggccggt tcgcccagcc
      241 cggcaacctc tccgtcgtga ggatcgtcga ggaatatcgc ccggggccgg ggcagcccgt
      301 agaacctgta ctggttggcg atgttcgcgg tgattatccc gccgaacgag tagccgtagt
      361 agctggtcct tccggtgtcc ggctggaccc ggttcgggct ttcacgcagg tactccaggg
      421 cagcgtgaat cccggtgact gccgagtcca tgcaggcctt gatgttgaag gggccggggc
      481 aggggtcgaa gtcactgatc tgccagcggg ggaagatcac gacgttgccc ttgaggaccg
      541 tgtgccggat cagttcatag agccggtcgt agccgctgaa ctccccgtag ccgtgggtga
      601 cgatcgccag cggcgcccgg gcgggcctcg gccctgtggg ttcaaaggcg atccaggcgt
      661 cgtagccttt accgccttca ctgacccgcc agccgctatg gctgtagtcg agacttccgg
      721 gacccttttc gagcggggag ggctggacga tcgtgcggcc ggaaaggacc tcggggagag
      781 cggcttccgc aggggtttca accgctgaca acagcagcag aaaggcgaca aatgcaagta
      841 ttgcggcggg catctctcct cctcggtccc ttcggcgtaa agtaccatac gccgatctcg
      901 cgaaggcggg attccgcttc cggcctgaag ccggggatca acggaagagg tcggtgaccc
      961 agatcgccag ttcgtcgctc agggcgatca gatccggctt ctcttcggtg tcgagggcat
     1021 cggaggccag ctgcctcgtg gtgtagatga tgccgatgta ggccgaccag ggcagctgct
     1081 ccgggccgcg cccggtcgga gaatcgtcgc tggagacacc ccagaggtga tgggcgaaga
     1141 gcaggaggga atcccttcgg cgcttgcgaa cggaagcgcc gagtgaatcg atctcgacct
     1201 ggtaggcgcg cgccaccacg aggtcgctct ggagagtctc caggtacgcc cggatcagcc
     1261 cgcagaccag ttcgggccgg gttctgctgc cgacgtcaac cgcgttcatg gcgtggaaga
     1321 gcacctcgat gaagcggtcg tagccggcca gggcacacgc ttccttgccg tcgaagcatt
     1381 cgtagaaggc ggccagcgaa acaccggcgt ggcgcgcgat ctcgtttgat ccgatgcccg
     1441 gtgcgccatg ggcggccatc agttcggtca tcgagacgag aatgcgttcc tgctggatcg
     1501 cgcggacctc ctcgccgctc agggaatgcc tgccgcgggg cagggcctga agcggttcga
     1561 agaagacacc ttcgcgaacg cgggggatca aatcatgcgg ggactggacc gccattggac
     1621 ggaatatacc catggccggg ggcggcgcat ccggtttcgg ccggcgggaa tcctgcggcc
     1681 cccgactccg ggacccggcg ttcccttggt cgttgtcacg tctgctacga tcggcgtaga
     1741 ggattgttct tcaaggagag gatggagatg tccaagcagg gtgaggggaa gggtgaggag
     1801 gatccgggag tccgtgaggt gaccccggcc gaggccggcg gcatcacccg gcggggcttc
     1861 ctggcccgtt ccgcagcgac cgctgcgacc gccgcgacgg tgggaagcct gaccggcgcc
     1921 ggcggggcgt ccgctgccgg gagcgagtcc cgcaaggtac ccgggagctt tggcgaggta
     1981 gccctgcccg atgcaaggca ggtcaaggcc gactatcagc ggatggtcga tttcggcccc
     2041 cggctccccg gctacgaaga acatctcgaa ttctgcaact ggatcgagga cgagttcgta
     2101 aaggcagggc tcgagctgct gcccaccgac cagatcccct accgccgctg gcggccgggc
     2161 aagatgtcgc tccgggtcgg caccaccgac tttgatcccg cgaccaccta cgtgcgggtt
     2221 cccggcactc cggcggaggg gatcacggcc cccatgatgc tggcaggcga cggcccggtc
     2281 gacgggaaga tcgtccttgt cgaggccgcg gtgccgaccc cgcgcaccgc cggcctcttc
     2341 atcgcgatgg ccgactactg gcagtggcgt ggacacacgg ccttcgaagc ggcggccgag
     2401 gaattcgacc gtttcgcctt ctcggactgg cccacgatca agccctacgc cgaccagggc
     2461 gccaaggcgg tgatcttcat aagccgggct tcgcgggccg aagtcgaagg caactggtca
     2521 cctcaccagg cggaagtcca gccgattccc ggcttcgtgg ttgaccgcga catgggggcc
     2581 gaactgaagg cgctcgccgc cggccagccc gaggcgaccc tgaagctgga agcggactgg
     2641 aaggacacct acgttccgca gatcacgggc atcctgccgg gcgagagcga cgaggtgctg
     2701 atcgtcaact cgcataccga cggccagaac gccttcgagg aaaacggggc tgtggccctc
     2761 gtccatctgg ccaggcattt cgcttcgctt cgcggcagcc ggaaactgaa gcgcacgctc
     2821 gtcttcatcg cctggcccgg ccacatggcc aacgtcagca atcgaccgga tgaccggaca
     2881 acgctgccgg agcagttcgg ctggatcagc tcgcaccggg atctctttga ccgcaccgtc
     2941 gccgcggtca cgatcgaaca cctcggggcc acacgctggt ccgacgaccc ggtcgagggc
     3001 taccactcga ccggaatgtc cgatttctac gcgatctgga ccaccctggg agccatgcag
     3061 gacctgtgca aggtcgccct gaaggagtcg gatctgcagc gccacgcgtt gctcaggcca
     3121 ccgatccagg ttacgcccgg cgtcgcctgg caccaccggg ggatcccgca cgtggccggg
     3181 atctcggcgc ccaagtacct ggtcagggtt tccgagaacg gcgacatcga caagcttgac
     3241 gcctccctgg cctcccggca gaccggcttc tacgccgacg tggtcaagtc gctggactcg
     3301 gccgaccctg cggccctcaa ggcgggtggt gaaggcgact caagcttcaa cagcgtgtcc
     3361 gatcgcgagt atccgatgac cagggcccgc tcggggccgg cacgggaagt ggtgctggac
     3421 gcgggcgacg gcggtcgcct cgcgatctac atcggaggcc gcgctgccgg cggccgggac
     3481 atcggcgtcc agatggcagc actcgaccac tcgctcgagc gggtcacggt ggcggtctac
     3541 cgcgacgaca ccctcctcgg caaatccaga ccgacgcgcg tcgaccgcaa agtcaagcgg
     3601 gtcgcgatcc accggagcgg acaccgggcc gaccaccgca atcttcacag gaaccggagc
     3661 gcggccagat tccgcgacgg cacctacaca ttcgtcgtcc gtcaggacgg caagcttctg
     3721 ggtcagcgga acgtcaccta ccagcggcgc cgctaggtac ccggacgagg gaaacaggac
     3781 tgcgatgact gcgcagcaca tgaacagcaa caaggagagg caatgaagga gacaactatt
     3841 caggacctgg gagggacagg aagggcgagg gtgtttctgc ttgccgcctt tgtcgcgctg
     3901 gccggcatgc ttggcatggc cggttcatca aaggccgaga cccacacgat cacggttgac
     3961 aacggacgct tgacgatggg tttcgtcttc ctcaacaaca agatcctccc ggccgacgtg
     4021 cccgggccgc tggatcccaa catcagcaac acctcgggca gcggcacgat cgccgtcgaa
     4081 accagtggct cgaccgccac ggtcgccgag gcggatttca acatgccgat cgtctggatc
     4141 ccggacccga ccgcgggcgg cgctccgatc ccgatgaagt tcggtcccaa aggggacctg
     4201 accggcacct gggacgccgg gacgggtcag ctctccctta cgggcacact ccaggttgac
     4261 gtg
//
LOCUS       DAOQFI010000061         4239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_9087_length_4239_cov_3.206501, whole genome shotgun sequence.
ACCESSION   DAOQFI010000061 DAOQFI010000000
VERSION     DAOQFI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9087_length_4239_cov_3.206501"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLE93_01850"
     CDS             complement(<1..333)
                     /locus_tag="PLE93_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85821.1"
                     /translation="MVRSVAIGLTGLLACFLIFALGTPAGAAASCKAASSSGGLSVGA
                     TKCKPFRKARLIRGKAYAPASAPARVKKVIAWANRIRNKPYIYGGGHGSFNDNGYDCS
                     GSVSYALRG"
     regulatory      complement(371..516)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            605..1312
                     /gene="rpiA"
                     /locus_tag="PLE93_01855"
     CDS             605..1312
                     /gene="rpiA"
                     /locus_tag="PLE93_01855"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HNH85822.1"
                     /translation="MKPARHREVTFEPFEASSISNYEAKFKAAEEVAEMAQSGQVIGA
                     GSGSTAFLAVRAIASRVRHGELHDIRLIPTSIESELTITGLGLVQADLAEVAPDWLFD
                     GADEVDPDGNLIKGRGGALFREKLLFSACQLRRVLIDPSKRVDRLGTSFAVPVEVVPR
                     AASVATASLKSLGATEIVIRTGSGKDGPVLTEQGNLLVDCRFETIEDSLEDEINLITG
                     VLDSGLFQNYAPEIIEA"
     gene            complement(1346..2140)
                     /locus_tag="PLE93_01860"
     CDS             complement(1346..2140)
                     /locus_tag="PLE93_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH85823.1"
                     /translation="MKPAGETRSVRRLEPAVILAVMGREFANFRTFWKSTAFTSTFEP
                     VVYLLAFGLGLGSTLVADVDGIDYVQFVGTGLVATAVVFSSALPAMFGTFVKERFQRT
                     YDAILAAPVDVEELVTAEMLWIGLRAAVFGCFPLIVTMFFGLEPRVTMLLVPIFAFVT
                     ALAFSAFGITMAALVSKIDQFNYVTTLVITPLFLVAGTFFPIDQLPQGFQIAAQFNPL
                     YQLVQLVRDACFGMEAVDALRFLGLLTLAFVLWRIAINRLTKRLIL"
     gene            complement(2137..3027)
                     /locus_tag="PLE93_01865"
     CDS             complement(2137..3027)
                     /locus_tag="PLE93_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH85824.1"
                     /translation="MPAIEIHDLKKSFGPINAVDGLDLVVDEGVCLGLLGPNGAGKST
                     TMKMLTGQAIPDSGVIRVLGYELPGQAKQARAAMGVVPQLDNLDIDVTVEENLAVFAR
                     LYRVDDVAAAVERTLEIARLTDRRKDAVDKLSGGMRRRLLLARGLVHEPKLLLLDEPT
                     VGLDPQIRTELWSLIDNLRSRGTTILMSTHYIEEAERLADEVAVVAGGKVIARATPKE
                     LIAEHAGEKTAEVYGPPERLTEVRSAAEEAGLRVRAAGPAVAIVGAEMAGPGLIPHDA
                     IHRAGSLEDVFVMLTGEDAE"
     gene            3105..>4239
                     /locus_tag="PLE93_01870"
     CDS             3105..>4239
                     /locus_tag="PLE93_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNH85825.1"
                     /translation="MIRGRIRDRAGHEEQSSADQVFEIDPGELTNVFSVPTWLRNAGL
                     GAWFLVGIAILLLSLVWLASLTEVIVVPVIVASIVGAVGSPLVSKMNGFGLPRAIGAI
                     LVMLLLVLVGAACAVMVVNGIVGEVPEISRDLDKATDDLSQFLTDQGIDEEQVEKAKE
                     GAKEGATASIGTLLSGVGSGIAGISSAFFFLAMVLLSSFFMLKDGPTIRGWAERATSL
                     PPPVARIVSGRMISSLRGYFAGVTIVAAFSAVVVMIGALIIGVPLVATIGAVTFVAGY
                     IPYLGAWTAGIFTVLLAYGSGGTDAAMGMAVVQLLANGILQQMIQPFAMGSTLGIHPL
                     AVLVVTIAGGALFGSIGLIIAAPLVAAAAGIVTDLKTAEERSGG"
BASE COUNT          742 a         1314 c         1433 g          750 t
ORIGIN      
        1 gccacgcagc gcgtagctga ccgaaccgga gcagtcgtaa ccgttgtcat tgaagctgcc
       61 gtggccgccg ccgtagatgt agggcttgtt gcgaatccgg ttggcccagg cgattacctt
      121 cttgaccctg gccggggcgc tggccggagc gtacgccttg ccgcggatta gccttgcctt
      181 gcggaacggc ttgcacttgg tcgcaccgac cgaaagaccg ccgctggaag aggccgcctt
      241 gcaggaggcc gcggcgccgg caggcgtgcc aagagcgaag atgaggaagc aggcgagcag
      301 accggtaagc ccgatcgcga cgctgcgaac catggatctc tgaatatgag gggttgaggg
      361 gatgatgccg atctcctttt tcagtgcctg cggagttagc tgacggacta gcgctttagg
      421 agtcgcgctc tcactcggtc gagtgggttc gccccaacta cctgggtccc ccgcttcccg
      481 ggccttgcgg caacgggaat tcggcaaatc ggtttttcag aatggtgaag tgtacagact
      541 ttactaatac tttgctaaca aaccagcgca aagccagaag tttctatgcc ctaggctctt
      601 gatcgtgaaa cctgcccgac accgagaagt cacgttcgaa cccttcgaag catcctcgat
      661 ttccaattac gaagccaaat tcaaggcggc cgaagaagtt gccgaaatgg cccagtccgg
      721 tcaggtgatc ggggccggct ccggctcgac cgcctttctg gcggtgcggg cgatcgcctc
      781 gcgggtgcgc cacggggaac ttcacgacat ccggctgatc ccgacctcga tcgagtccga
      841 actgacgatc accggccttg gcctggttca ggccgatctg gccgaggtgg cgcccgactg
      901 gctgttcgac ggggccgacg aggttgatcc ggacggcaat ctgatcaagg gtcgcggcgg
      961 cgcgctcttt cgggaaaagc tgctcttttc ggcatgtcaa ctgcgccggg tgctgatcga
     1021 cccgtcaaag cgggtggacc ggctgggcac cagcttcgcg gtgccggtcg aagtcgtgcc
     1081 gcgggccgca tccgtcgcga ccgcctcgct caagtcgctc ggagccaccg agatcgtgat
     1141 ccggaccgga tccggcaagg acggcccggt cctgaccgaa cagggcaatc tcctggtcga
     1201 ctgccggttc gaaacgatcg aagacagtct cgaggatgag atcaacctga tcaccggcgt
     1261 cctcgactcc ggcctcttcc agaattacgc tcccgaaata atcgaagcct gagcccctgg
     1321 ccggcgaaga ccggcccgac tgcagtcaaa ggatgaggcg tttggtgagg cggttgatcg
     1381 cgattcgcca gaggacgaag gcgagggtca gcaggccgag gaagcggagg gcgtcgaccg
     1441 cctccatgcc gaagcaggca tcgcgcacca actgaacgag ctggtagagc gggttgaact
     1501 gggccgcgat ctgaaaaccc tggggcaact ggtcgatcgg gaagaaggtg ccggcaacca
     1561 gaaagagcgg cgtgatgacc agggtcgtga cgtagttgaa ctggtcgatc ttcgaaacca
     1621 gggcggccat ggttatcccg aaggcggaaa aggcgagcgc cgtgacgaag gcaaaaatcg
     1681 ggaccagcag catggtcacc cggggctcga gcccgaagaa catcgtcacg atcagcggga
     1741 agcagccaaa gaccgctgcc cgcaggccga tccagagcat ctccgcggtg accagttcct
     1801 cgacgtcgac cggcgccgcg aggatcgcgt cataggttcg ctggaagcgc tccttgacga
     1861 aggtgccgaa catggccggc agggctgaag agaagacgac cgcggtcgcg accaggccgg
     1921 tgccgacgaa ctggacgtag tcgatcccgt cgacgtcggc aaccagggtc gaaccgagac
     1981 caaggccaaa ggccagcagg tagacgaccg gctcaaaggt gctggtgaag gcggtcgact
     2041 tccagaaagt ccggaagttg gcgaattccc ggcccatcac ggccaggatc acggccggtt
     2101 cgagccggcg caccgagcgg gtctcgccgg ccggcttcat tcggcgtcct cgccggtcag
     2161 catcacgaag acgtcctcga gcgagccggc ccggtggatg gcgtcgtgcg ggatcagccc
     2221 cggtccggcc atctcggctc cgacgatcgc gaccgccgga ccggccgccc ggaccctgag
     2281 accggcttcc tcggctgccg agcggacctc ggtcagccgc tcgggcgggc cgtagacctc
     2341 ggcggtcttc tcgccggcat gttcggcgat cagttccttc ggggtggcac gggcgatcac
     2401 cttgcctccg gcaaccaccg cgacctcatc ggccagcctt tcggcttctt cgatgtagtg
     2461 agtggacatc aggatcgtgg tgccgcgtga gcgcaggttg tcgatcaggc tccagagctc
     2521 ggtccggatc tgggggtcga ggccaaccgt gggctcgtcg agcagcagca gtttcggctc
     2581 gtggaccagg ccccttgcca gcaggagccg gcggcgcatg ccgccggaga gcttgtcgac
     2641 ggcgtccttg cgacgatcgg tcagccgggc gatctcgagc gttcgctcga ccgcggcggc
     2701 gacatcgtcg acccggtaga ggcgggcgaa tacggcaagg ttctcctcga ccgtcacgtc
     2761 gatgtcgaga ttgtccagct gcggcacgac ccccatcgcc gcacgcgcct gcttggcctg
     2821 ccccggcagt tcgtagccga gtacccggat cacgccggaa tcgggaattg cctgcccggt
     2881 aagcatcttc atcgtggtcg acttgccggc gccgttcgga ccgagaaggc ccaggcagac
     2941 accttcatcg acgaccagat cgagtccatc gacggcgttg atcgggccga agctcttctt
     3001 caggtcgtgg atttcgatcg cgggcatcgg ggcagtcaat cagaccggcg aacccggccc
     3061 cgtggtcccc ggaaccgtct ggctccggcg gtagcctgcg agccatgatc aggggcagaa
     3121 tcagggaccg ggcggggcac gaggagcagt cttccgccga ccaggtcttc gagatcgatc
     3181 ccggcgagtt gaccaacgtc ttttccgtgc cgacctggct gaggaacgcc gggctgggcg
     3241 cctggtttct ggtcggcatc gcgatcctgc tgctttcgct ggtctggctt gcctcgctga
     3301 ccgaggtgat cgtggtgccg gtgatcgtcg cctcgatcgt cggtgcggtc ggctcgcccc
     3361 ttgtttcgaa gatgaacggg ttcggtctgc cgcgggcgat cggcgcgatc ctggtcatgc
     3421 tgctgctggt cctggtcggt gccgcctgcg cggtgatggt ggtcaacggg atcgtcggcg
     3481 aggtccctga gatcagccgg gacctggaca aggccaccga cgacctgagc cagttcctga
     3541 ccgaccaggg aatcgacgag gaacaggtcg agaaggccaa ggagggtgcc aaagaaggag
     3601 caactgccag catcgggacc ctgctttccg gggtcggttc gggcatcgcc gggatttctt
     3661 cggccttctt cttcctcgcg atggtcctgc tcagctcgtt cttcatgctg aaggacggtc
     3721 cgacgatcag gggctgggcg gagcgggcga cgagcctgcc gcctccggtg gcacggatcg
     3781 tttcgggccg gatgatttcg tcgctgcggg gatatttcgc cggggtcacg atcgttgccg
     3841 ccttcagtgc cgtggtggtg atgatcgggg cgctgataat cggagtgccg ctggtcgcga
     3901 cgatcggcgc cgtcaccttc gtggccggct acatcccgta cctcggtgcc tggacggccg
     3961 gaatcttcac cgtgctgctg gcctacggat ccggtggaac cgatgccgcg atggggatgg
     4021 cggtcgtcca gctgctcgcc aacggaatcc tccagcagat gattcagccg ttcgcgatgg
     4081 gcagcaccct cggcatccat cccctggccg tgctggtggt caccatcgcc ggtggcgcac
     4141 tgttcggatc gatcggcctg atcatcgccg cgccgctggt tgccgcggcg gccgggatcg
     4201 tgaccgacct caagacggcc gaggagagat caggtggtc
//
LOCUS       DAOQFI010000062         4141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_9432_length_4141_cov_2.540382, whole genome shotgun sequence.
ACCESSION   DAOQFI010000062 DAOQFI010000000
VERSION     DAOQFI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9432_length_4141_cov_2.540382"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLE93_01875"
     CDS             <1..256
                     /locus_tag="PLE93_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH85826.1"
                     /translation="RGEATRLANGSRYGLAAAIWTSDFGRAHRVARALKAGTVYINCY
                     DRGDNSLPFGGFKESGIGVDRSLAAMEKYSNLKTIWADIS"
     gene            274..1296
                     /locus_tag="PLE93_01880"
     CDS             274..1296
                     /locus_tag="PLE93_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNH85827.1"
                     /translation="MTDGRVTLQDVASLAGVSQATAGRALGGYGSISEDSRQRVEQAA
                     TKLGYRTNAVARALASGTTKSIGLVIGDIENPFFATVARGLSDVVQEAGYTVVLANAD
                     ENWDMERQALEVFDTRSVDGLVVAPAPGSDASSIEKLGNRIPLVVIDRDLPGLDVDSV
                     LVDNVAGAASATRHLVNLGHREIGLVTEPPVISSVRERIQGYEQVLEENGLATSPSLR
                     AEGTPRLEGGLLAATELLNSPDRPHAIFATTNTMTAAVLAAAAELGLKIPEDLSLVGF
                     DDSEWMRLVSPPITVVRQPVYALGQATGRLLNDRINGWTGDPKQLRLKTELVVRESCR
                     KWQEPS"
     gene            1343..2356
                     /locus_tag="PLE93_01885"
     CDS             1343..2356
                     /locus_tag="PLE93_01885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNH85828.1"
                     /translation="MLNFDVDRYLDIQSGAVNLAEGLDRTIGDLLDGGKKTLIFAGAG
                     GAGMLMQPAAQLLMRRSRFPVFWDRPAEMMAIRPVYFDENAVVIIPSLSGTTMESIEL
                     VEASREVGAVTIALTGHEDSPIAGSVDHNFTNFAEDDTSSESFYLQSLLVALSVLRRE
                     PDRVDYDSVVSELKKLPELLVDAKAAFEDRAEEWAEAFRDEDYHIISAAGPLWAEAWY
                     YGTCILEEMQWIRTRPIHAADFFHGTLELLEPEVSLILLKGSGPSRELENRVEEFAGT
                     ISNKVRVLDTDDVDMPGISDPVREMVSPAIASALLERVSAHLEVKRDHPLTTRRYYRK
                     VAY"
     gene            2614..2907
                     /locus_tag="PLE93_01890"
     CDS             2614..2907
                     /locus_tag="PLE93_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85829.1"
                     /translation="MIGSRAREFMWMFEVEMEDGTAVQAYKHVFTRKYFHLGSDGRSF
                     EFRSFGGEGPSGYEQVPFLRTAGMVLWLYDQERETLWKPGEREYCNCPLNCWD"
     gene            complement(2904..3329)
                     /locus_tag="PLE93_01895"
     CDS             complement(2904..3329)
                     /locus_tag="PLE93_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNH85830.1"
                     /translation="MEELYREQILEHYKRPHNFGAVENPDLEFEDTNPFCGDEQHVTI
                     RLDEDDRVAEVKFEGQGCAISTAATSLLTDELIGMSRDELIKLPKEYVLDLLGIDISA
                     TRMKCALLGLKVVKSAGLGQTTDWEDAGEQGDPSLASEI"
     gene            complement(3383..3676)
                     /locus_tag="PLE93_01900"
     CDS             complement(3383..3676)
                     /locus_tag="PLE93_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85831.1"
                     /translation="MDPEDDLVIFPREDVEACLRNEKMQQEANSDGSPSGQSQAMETS
                     RLGAIEAGAPERIRWIYFESPEWTWQNLCGRSGWLLIDPETIDQYDFVMTMMN"
     gene            complement(3720..>4141)
                     /locus_tag="PLE93_01905"
     CDS             complement(3720..>4141)
                     /locus_tag="PLE93_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85832.1"
                     /translation="SVDITVTVPGLFSRDLSARRFRNLRHDLFVEVEWLKKGRWLNTD
                     QLVKRCGRIQQNLINLQRSIDSGRCEFAAMLIVDDEGFLDFHGDLLRWPPDVIPLVVS
                     PGEVVELGLDDGSVEPVLDEIESLHDSACPCSDGPLA"
BASE COUNT          792 a         1258 c         1308 g          783 t
ORIGIN      
        1 acggggcgag gccacgcgac tcgccaatgg ctcgagatac ggactcgccg ccgcgatctg
       61 gacctcggac tttggccggg cccaccgcgt cgcgcgcgcc ctcaaggcgg gaaccgttta
      121 catcaactgc tacgaccgcg gcgataattc acttcccttc gggggtttca aggagtcggg
      181 gatcggcgtt gaccggtcgc tcgccgcgat ggagaaatac tcgaacctga agacgatctg
      241 ggccgacatt tcatgagcga aagagacgga aacatgaccg acggtcgggt gactcttcag
      301 gatgtggcct cgctcgccgg tgtttcccag gctacggccg gccgggcact gggcggctac
      361 gggtcgatca gcgaggattc ccgccagcgc gtcgaacagg cggcgaccaa gctggggtac
      421 cgaaccaacg ccgtcgcccg ggcgctcgcc tcgggcacga ccaagagcat cgggctggta
      481 atcggcgaca tcgaaaatcc gttcttcgca actgtggccc gcggactttc cgatgtagtt
      541 caggaagcgg gctacaccgt ggttctggca aacgccgacg aaaactggga catggaacgt
      601 caggcgctcg aggttttcga cacccgctcg gtcgacggtc tggtcgtcgc tcccgcgccc
      661 ggttcggacg cgagttcgat cgagaagctg ggcaaccgga ttccactggt cgtgatcgac
      721 cgcgacctgc ccggacttga cgtcgactcg gtgctggtcg acaacgttgc cggggccgcc
      781 agcgcgaccc gacatctggt caatctcggc caccgcgaaa tcgggctggt taccgagccg
      841 cccgtcatct cgtcggttcg cgagcgaatc cagggctacg agcaggtgct cgaggaaaac
      901 ggtttagcga cttccccgtc cttacgagcg gaaggcacgc ccagacttga aggcggcctg
      961 ctcgctgcca ctgaactgct gaactctccc gatcgccccc acgcgatttt cgccaccacc
     1021 aacacgatga ccgcagcggt tctcgcggcg gcggcggaac tcggactgaa gattcccgaa
     1081 gacctgtcgc tcgtcgggtt tgacgattcg gagtggatgc ggctcgtgtc cccgccaata
     1141 accgtggtcc ggcaaccggt ctatgcgctg ggccaggcaa ccggacggct gctgaacgac
     1201 cggatcaacg gctggacggg agatccgaaa caactccgcc tgaaaaccga actggtcgtc
     1261 cgggaatcct gccgcaagtg gcaggagcct tcctgaccca ggtcaataac cccgatcaac
     1321 atgcgcatga acaaggtgaa tgatgcttaa tttcgatgta gacagatacc tggatatcca
     1381 gtccggcgcg gtcaacctcg ccgaaggact ggaccggaca atcggcgacc tgctggacgg
     1441 gggcaagaaa accttgatct tcgccggagc gggaggggcc gggatgctga tgcagccggc
     1501 ggcgcaactg cttatgcggc gatcccgctt cccggtcttc tgggaccggc cagccgaaat
     1561 gatggcgatc aggcccgttt acttcgacga gaacgcggtc gtgatcatcc cctcactgtc
     1621 agggaccacg atggagagca tcgaactggt cgaagccagt cgggaggtcg gcgccgtcac
     1681 tatcgccctg accggtcacg aggattcacc catcgccggg tcggtcgatc acaacttcac
     1741 caacttcgcc gaagatgaca cctcgtcgga gtcgttctat ctccagtccc tgcttgtcgc
     1801 gctttcggtg ctccggcggg agccagacag ggttgactac gactcggtcg tcagcgaact
     1861 gaagaagctt cccgaactgc tcgttgacgc aaaggccgct ttcgaagatc gcgccgagga
     1921 gtgggccgaa gcctttcgcg acgaggatta ccacatcatt tccgccgccg ggccgctctg
     1981 ggcggaagcc tggtactacg ggacctgcat tctcgaggag atgcagtgga tccggacccg
     2041 gccgattcac gcagcggatt tcttccacgg cacgctcgag ttgctcgagc ccgaagtcag
     2101 cttgatcctg ctgaagggaa gcggaccctc tcgggaactc gagaacaggg tcgaggaatt
     2161 tgccgggacg atctcaaaca aggtacgagt cctggacacg gacgatgtcg atatgccggg
     2221 gatatcggac cccgtacggg aaatggtctc acccgcaatt gcctccgcgt tgctggaacg
     2281 ggtcagcgcc cacctcgaag tcaagcggga tcatccactg actacccgcc gctactacag
     2341 gaaagtcgcc tactgaagcc ctttgagaac caggacgtgg attgaatcac ccagtgagcg
     2401 aatcgaaggc gagaagctgg atcgagtttc cccgcgcatt tcgcccgtct gctgcgcttt
     2461 cgtctccatt tcgtcaccca attgttgcta tgaagtcttg tttcgtcaca ccttttcgcg
     2521 catggtcggg gcatgccgaa gaagaccgaa acaccacttg ttatgggtac tgtctcccaa
     2581 cggcgagagc ccgtctggga tcccctcgaa tacatgattg gctcgcgagc ccgcgagttc
     2641 atgtggatgt tcgaggtcga gatggaggat gggacggcgg tccaggccta caagcacgtc
     2701 ttcacgcgaa agtacttcca cctggggagt gacggtcgat cgttcgagtt ccgaagcttt
     2761 gggggcgaag ggccctcggg gtatgagcag gttcccttcc tgaggacagc cggaatggtg
     2821 ctttggctgt atgaccagga gcgggagact ctttggaaac ccggcgaacg tgagtactgc
     2881 aactgcccgc tgaactgctg ggactaaatc tcggaggcca gggaggggtc gccttgttct
     2941 ccggcgtcct cccagtcggt tgtctggccg aggccggctg acttgaccac cttgaggcct
     3001 aggagggcgc acttcatgcg ggtggcggag atgtcgattc cgaggaggtc aaggacgtac
     3061 tccttgggga gcttgatcaa ttcgtcgcgg gacatgccga tgagctcgtc ggtgaggaga
     3121 gaggtggctg cggtggagat cgcacagccc tggccttcga acttgacctc ggcgacccgg
     3181 tcgtcttcgt cgaggcgaat cgtgacgtgc tgctcgtcgc cgcagaaggg gttcgtgtcc
     3241 tcgaactcga ggtccgggtt ctcgaccgcc ccgaagttgt gggggcgctt gtaatgctcg
     3301 aggatctgtt cgcggtagag ctcttccatc ggtcagacga ggttagctga ccgccgctcc
     3361 caggccacga acccttgccg ggtcagttca tcattgtcat cacgaagtcg tactggtcaa
     3421 tcgtttccgg atcgatgagc aaccagccgg agcgcccgca aaggttctgc caggtccatt
     3481 cgggtgactc gaagtagatc caccggattc gctcgggtgc tccggcctca atcgcgccga
     3541 gccgactcgt ttccatcgcc tgactctgcc cggacggaga accgtccgaa ttggcttcct
     3601 gctgcatctt ctcgttccgc aaacacgctt caacgtcttc ccggggaaag atcaccaggt
     3661 catcttccgg gtccatgagt tgagagtcgc gagcccggcc cggacctgac agggtccggt
     3721 caggccaggg ggccgtcgga gcaggggcag gcggagtcgt ggagggattc gatttcgtcg
     3781 aggaccggtt cgaccgagcc gtcgtcgagg ccgagctcaa ccacctctcc cgggctgacc
     3841 accaggggaa tcacgtccgg cggccacctg agaaggtcgc cgtggaagtc gaggaagcct
     3901 tcgtcgtcga cgatcagcat cgcggcgaac tcgcagcgcc cgctgtcgat cgagcgctgc
     3961 aggttgatca ggttctgctg gatccggccg cagcgcttga ccagctggtc ggtgttcagc
     4021 cagcggccct tcttcagcca ctcaacctcg acgaagagat cgtggcgcag gttccggaac
     4081 cggcgtgcgc tcaggtccct ggagaacaat ccgggcacgg tgaccgtgat gtccacggac
     4141 t
//
LOCUS       DAOQFI010000063         4123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_9504_length_4123_cov_3.376106, whole genome shotgun sequence.
ACCESSION   DAOQFI010000063 DAOQFI010000000
VERSION     DAOQFI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9504_length_4123_cov_3.376106"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLE93_01910"
     CDS             <1..404
                     /locus_tag="PLE93_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85833.1"
                     /translation="AFPGELIFQLAFWGAALAILIGAAFLLAVVWERLSTEGSPPSPA
                     SEPEPKPDPDPEPEPEPEPEPAPEAEPAPESGKVKAAKKELRRAPQTDLVAATLKKSK
                     KTKKPAKKSPKDPKASKAPKKPKKKDSKKKK"
     gene            425..1414
                     /gene="rlmN"
                     /locus_tag="PLE93_01915"
     CDS             425..1414
                     /gene="rlmN"
                     /locus_tag="PLE93_01915"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNH85834.1"
                     /translation="MDLTLLEETMAGLEQPAYRTGQVWEWLARGAGSFDEMTNLPASL
                     RQELEGRLPISTLEVDASMKSVDGTEKALFLTADGRPVEAVLMRYRDGRRSLCLSSQS
                     GCPLTCTFCATGTMKFGRNLTESEILDQALHFRRIEPVNHAVFMGMGEPMMNLDNVLA
                     VCERLPEVGIANSHTTVSTVGWQPGIERMTTEGPAVRLALSLHAPNDKLRSEIMPVND
                     RYPMEDVVAACREWRHTRKRKVFIEYLMLDGVNDSVELAHELADLLLPKNDFKVNLIP
                     YNPSGTGYQGSPREAIDRFRDTLMKRGIHATVRLTRGRDIDAACGQLAAKAAA"
     gene            complement(1524..2015)
                     /locus_tag="PLE93_01920"
     CDS             complement(1524..2015)
                     /locus_tag="PLE93_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNH85835.1"
                     /translation="MTEILNVLVEVPKGSRNKYEYDEELGAIKLDRFLFSSMVYPVDY
                     GMVPNTMGADGDPLDAMVCVSEPTFPGCIIPAIPLGLFRMTDDGELDDKILCVPADDP
                     NWNFIESLDQLSSQLKDEISHFFAVYKQPEGKHVEVDGWYPLESALEVLELSRERFAS
                     GTA"
     gene            complement(2087..3523)
                     /gene="zwf"
                     /locus_tag="PLE93_01925"
     CDS             complement(2087..3523)
                     /gene="zwf"
                     /locus_tag="PLE93_01925"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNH85836.1"
                     /translation="MSKTKEREATDALVIFGITGDLARKMTFRALYRLEKRGKLDCPI
                     IGVGRNQDWHHETMKQRAEEAIRGSVDEFDLATFRRLADRMRFVNGNFDEEDLYQSLG
                     GELADYERPLFYLEIPPPLFSEVVHGLANAGLVENARVILEKPFGHDFDSAQELQADL
                     LTVLEEDQILRIDHYLGKEPVMDITYLRFANALLEPVWNRNYVSYVQMTMSENFGVED
                     RGRFYEGVGAIRDVVQNHMLQVLALLAMEAPNANQHDSIRAKKLELFEAIRSADPAKC
                     VRGQYNGYRDIPDVADDSQVETYAALELAIDNWRWSGVPFYLRTGKNLPVKHTEVRVV
                     FKRPPHTGVGMKSHKPPRNQMVVRIDPVPGARMAFVAKAAGIEDFEEADLDVLFEKEP
                     GAEPEPYERLLDDAIHGRYDLFTKFSMVEETWRIVQPLLDDPPPVVPYEVGSWGPEEA
                     DRLVKGICDWDEPWRPTEDPRHHMLLGG"
     gene            3580..>4123
                     /locus_tag="PLE93_01930"
     CDS             3580..>4123
                     /locus_tag="PLE93_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH85837.1"
                     /translation="MEIGIVGLGRMGANIARRLMRDGHRCVVYDVNPEAAKDLAGEGA
                     DPAADLAELAAKLAAPRAVWIMVPAGDITRDTIRKVAAELQPGDAIIDGGNTYYHDDI
                     HFAEELGKQGIDHLDCGTSGGVFGLERGYCLMIGGPEEAYKRLEPIFATLAPGVGEAE
                     RTPGLTGDPSPAENGYLYCGP"
BASE COUNT          753 a         1339 c         1335 g          696 t
ORIGIN      
        1 tcgccttccc gggcgagctg atcttccagc tggccttctg gggcgcggcg ctggcgatcc
       61 tgatcggcgc ggcattcctg cttgccgtcg tctgggagcg gttgagcacg gaaggatctc
      121 caccatcccc ggcctcggaa ccagaaccaa agcccgaccc cgacccggaa ccggaacccg
      181 aaccggaacc cgagcccgca cctgaagcag agccggcgcc ggagtccggc aaggtgaaag
      241 ccgcgaagaa ggaactgcga agggcaccac agaccgatct cgtcgccgca acgctcaaga
      301 agtcaaagaa gacgaagaag ccggcaaaga agagcccgaa ggatccaaag gcttcgaagg
      361 caccgaagaa gccgaagaag aaggactcaa agaagaagaa gtgagctcct gccacactct
      421 tggtgtggat ctgacgcttc ttgaagaaac gatggcgggc ctggagcagc cggcctaccg
      481 gaccggtcag gtctgggagt ggctggcccg cggggccggt tcattcgacg agatgacgaa
      541 cctgcccgcc tcgctgcggc aggaactcga aggcaggctg ccgatctcga ccctggaagt
      601 cgacgcctcg atgaagtcgg tcgatggcac cgagaaggcg ctctttctga ccgccgacgg
      661 ccgcccggtc gaggcggtac tgatgcgcta ccgcgacggc cgccgttcac tctgcctctc
      721 cagccagtcg ggctgcccgc tcacttgcac cttctgcgcc accggcacga tgaaattcgg
      781 ccgcaacctg accgagtcgg agatcctcga ccaggccctt cacttccggc ggatcgagcc
      841 ggtcaaccac gccgtcttca tgggcatggg cgagccgatg atgaacctcg acaacgtgct
      901 ggccgtctgc gaaaggctgc cggaagtcgg gatcgccaac tcccacacca cggtttcaac
      961 cgtcggctgg cagcccggga tcgaacgcat gacgacggaa ggcccggcgg tccgcctcgc
     1021 cctctcgctt cacgccccga acgacaagct ccgctccgaa atcatgcctg tcaacgaccg
     1081 ctacccgatg gaggacgtgg tcgccgcctg ccgcgagtgg cgccacaccc gcaagcgcaa
     1141 ggtctttatc gagtacctga tgctcgacgg ggtaaacgac tcggtcgagc tcgcccacga
     1201 actggccgac ctgctccttc cgaagaacga cttcaaggtc aacctgatcc cctacaaccc
     1261 gtcgggaacc ggctaccagg gctcaccccg cgaagcgatc gaccgcttcc gcgacaccct
     1321 gatgaaacgc ggaatccacg ccaccgtccg cctcacccga ggccgcgaca tagacgccgc
     1381 ctgcggccaa ctggcggcca aggccgcagc ctgaccggac caggttcttc ccgccccgag
     1441 cggagtccct tccttccatc cgtgaatgtg agaggaaggg acgggtttct cgtggagggc
     1501 gggtcggacc ccgcccgact tacttatgcg gttccggagg cgaagcgttc gcggctgagt
     1561 tcgaggactt cgagggcgga ctccaggggg taccagccgt cgacttccac gtgcttgcct
     1621 tcgggctgct tgtagaccgc gaagaagtgg gagatttcat ccttgagctg gctcgagagc
     1681 tggtcgagcg actcgatgaa gttccagttc gggtcgtcgg ccgggacgca gaggatcttg
     1741 tcgtcgagct cgccgtcgtc ggtcatgcgg aaaaggccga gcgggatcgc cgggatgatg
     1801 cagccgggga aggtcggttc ggaaacgcaa accatggcgt cgagcgggtc gccgtcggcg
     1861 cccatcgtgt tcggcaccat gccgtaatcg acggggtaga ccatcgagga gaagaggaaa
     1921 cggtcgagct tgatcgcgcc cagctcttcg tcgtattcgt acttgtttcg ggagcccttc
     1981 gggacttcca ccagaacgtt caggatctca gtcatatcgc cgcgaatcct atcttttaag
     2041 gagtcaggcg gggtccgacc cgccctccat ggcacctccg gtggctttag ccgcccaaga
     2101 gcatgtgatg cctgggatcc tcggtcgggc gccagggttc atcccagtcg cagatgccct
     2161 tgaccaggcg atcggcctcc tccgggcccc agctgccgac ctcgtagggg acgaccgggg
     2221 gcggatcatc gagcagcggc tgcacgatcc gccaggtctc ctcgaccatg gagaacttgg
     2281 tgaagaggtc gtagcggccg tggatcgcgt catcgagcag gcgctcgtag ggctccggtt
     2341 cggctcccgg ctccttctcg aagagcacgt cgaggtccgc ttcctcgaag tcctcgatac
     2401 cggcggcctt ggcgacgaag gccatgcgcg ccccgggcac cgggtcgatc cggaccacca
     2461 tctggttgcg cggcggcttg tgggacttca ttccgacccc ggtgtggggc ggcctcttga
     2521 agaccacccg gacctcggtg tgcttgaccg gcaggttctt gccggtccgc aggtagaagg
     2581 ggacccccga ccagcgccag ttgtcgatcg ccaactccag cgcggcgtag gtctcgacct
     2641 gggaatcgtc ggcgacgtcc gggatgtccc ggtagccgtt gtactggccg cggacgcatt
     2701 tggccgggtc ggccgagcgg atcgcctcga agagctcgag cttcttggcc cggatcgagt
     2761 catgctggtt cgcgttcggg gcctccatgg ccagcagggc cagcacctgg agcatgtggt
     2821 tctggaccac gtcgcggatc gctccgaccc cctcatagaa gcggccgcgg tcctcgaccc
     2881 cgaagttctc gctcatggtc atctggacat aggagacgta attgcggttc cagaccggtt
     2941 cgagcagcgc gttggcgaag cggaggtagg tgatgtccat caccggctcc ttgccgaggt
     3001 aatggtcgat cctgaggatc tggtcttcct cgagcacggt cagcaggtcg gcctgaagtt
     3061 cctgggccga gtcgaagtcg tggccgaagg gcttctcgag gatgacccgg gcgttttcga
     3121 ccagaccggc gttggcgaga ccgtggacca cctcggagaa gagcggcggc gggatctcga
     3181 ggtagaagag cggccgctcg tagtcggcca gctcgccacc gagcgactgg tagagatcct
     3241 cctcgtcgaa gttgccgttg acgaagcgca tgcgatcggc gaggcgccgg aaagttgcga
     3301 gatcgaactc gtcgaccgaa ccgcgaatcg cctcctcggc ccgctgcttc atggtctcgt
     3361 ggtgccagtc ctggttgcgg ccgacgccga tgatcgggca gtccagcttg cctcgctttt
     3421 cgagccggta aagggcccgg aaagtcatct tcctggcgag atcaccggtg attccgaaga
     3481 tgaccagcgc gtcggtcgct tccctctcct tggtcttgct catgaccggc agcgtatcca
     3541 tccgacgaaa tgttccacca cgggttctaa tatggcggca tggaaatcgg gatcgtcggg
     3601 cttggtcgga tgggggcgaa catcgcccgg aggttgatgc gcgatggaca caggtgcgtg
     3661 gtctatgacg tgaatcccga agccgcgaag gatctggccg gtgaaggagc cgatccggcc
     3721 gccgacctgg ccgagctggc cgccaagctg gccgccccgc gcgcggtctg gatcatggtc
     3781 ccggccggag acatcacccg cgacacgatc cgcaaggtcg cggccgaact tcagcccggc
     3841 gacgcgatca tcgacggcgg caacacctac taccacgatg acatccactt cgccgaagaa
     3901 ctgggcaaac agggcatcga ccaccttgac tgcggcacct ccggcggcgt tttcggcctg
     3961 gagcgaggct actgcctgat gatcggcggg ccggaagagg cctacaagcg cctcgagccg
     4021 atcttcgcca cgctcgcccc cggggtcggc gaggcggaac gcaccccggg gctcaccgga
     4081 gatccctccc cggccgagaa cggctacctc tactgcggcc cgg
//
LOCUS       DAOQFI010000064         4072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_9685_length_4072_cov_3.318895, whole genome shotgun sequence.
ACCESSION   DAOQFI010000064 DAOQFI010000000
VERSION     DAOQFI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9685_length_4072_cov_3.318895"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLE93_01935"
     CDS             <1..271
                     /locus_tag="PLE93_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85838.1"
                     /translation="EGADDQGRTASRFEIGCEVTSARRMARGRLTGTDVYGLTAALIV
                     DGAIRASRGEVKGSGALAPAVAFDPATFLSGLDRFGFNWEVTPPR"
     gene            282..842
                     /gene="thpR"
                     /locus_tag="PLE93_01940"
     CDS             282..842
                     /gene="thpR"
                     /locus_tag="PLE93_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNH85839.1"
                     /translation="MSGSSRQNRKRRVFVALDPPVELRSEAEAWARHIARSAKGLRVV
                     PARNSHITLAFLGERDEFEIEEVAGALLETAVPATGLALGAPAWLPKRRPRSLVLDIH
                     DDRGELKELQAGMAGALKSALGWKEDRPYRPHLTCARTGRGFDPSHLRVPVSPSIAFS
                     GQSVTLYASHLGPEGAEYEVIERVDQ"
     gene            complement(1092..1382)
                     /locus_tag="PLE93_01945"
     CDS             complement(1092..1382)
                     /locus_tag="PLE93_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85840.1"
                     /translation="MAVAPFNLKVPAVFSNVPNIGSSPAGEDCTAPGHKSGGHAGSQS
                     TLGAAANRKDGPARRQKAILVLTMLDHRLGKARSEHLRSGHKPELLPRDPLE"
     gene            1434..2021
                     /locus_tag="PLE93_01950"
     CDS             1434..2021
                     /locus_tag="PLE93_01950"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNH85841.1"
                     /translation="MRVRRSRYFNPAQVTRKYGIPILRPSWILLDLARELPDDEFGHA
                     FKEADRLGLLNEEELRACLELGSGLEGIRKFQNLVARRHPDLKDVRTLLETLFLELCA
                     RYGIETPRVNRRKGRYFPDFSWDDIGLIVEVDGYEGHAGRFAFLDDAARENDLRRLGY
                     QVIRFTWEEITEHPELVASLVIREMARCRALARVA"
     gene            complement(2227..3858)
                     /locus_tag="PLE93_01955"
     CDS             complement(2227..3858)
                     /locus_tag="PLE93_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85842.1"
                     /translation="MSFRRFFVAFLMLAAILVLPQSASALLPHSTTKRLDDGTERITY
                     RIGPLTVTPGQNRIAFKPLTGIEKPSVDGWITRIKPNMVNEDGSIPLSSKVMFHHGVW
                     LNFSRQDATSGGQERFFATGEEKTITRFPDGYGYQYKASDVWLLNHMIHNLTPEAMTL
                     YATYTIDFIPADSPAADGIKPVTPIWMDVDNGSVYPVFDIWKGSGGKDGKFTYPDDAD
                     NPYPNGVAKNTWTVDRDGVLLATAGHVHTGGLYTDLYLQRPGARYAGPKCVQPKAARP
                     PNRRNIRRYGAKLKKYKSCKASMPSVKGSKVHLFRSQAHYFEPAGPVSWDMAMVGTRD
                     DWRPEVKKGDVLSMNSTYETKLASWPESMGIMVVYMAEGEHGTNPYRKKVDYKGQLIH
                     GHYAENNDHGGKLPVVGPDPTRLPDGANPDGNPFTISGFSFNGGDFRLPGAAGRPPVV
                     DEGKSLTFQLNQDDINDEIWHSLTSCKTPCNKSTGIAYPIPDGKFTFDSGQLGVGGAP
                     TVERTSWKTPADLPRGTYTFFCRIHPLMRGAFRVK"
     gene            complement(3863..>4072)
                     /locus_tag="PLE93_01960"
     CDS             complement(3863..>4072)
                     /locus_tag="PLE93_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85843.1"
                     /translation="PYGKSASASRKVTLGTSRALDFTAGQSKTVNLPLTKAGRKAIAR
                     PKSVQIKLSAAVPYGKSASASRKVK"
BASE COUNT          682 a         1248 c         1362 g          780 t
ORIGIN      
        1 ggagggcgca gacgaccagg gccgtaccgc cagccggttc gagatcggct gcgaagtcac
       61 ttccgcccgg cgcatggcac gcggccgcct gaccgggacc gatgtctacg ggctcaccgc
      121 ggcgctgatc gtcgacggcg cgatccgggc atcccgcggc gaggtcaagg gctccggcgc
      181 cctggccccg gccgtcgcct tcgatccggc caccttcctt tccgggctcg accgcttcgg
      241 attcaactgg gaggtaaccc cgccccgcta acctcaggcg ggtgagcggg agcagtcgac
      301 agaaccgcaa gcgccgggtc ttcgtggccc ttgacccgcc ggtcgagttg cgctccgaag
      361 cggaggcctg ggcccgtcac atcgcccgca gcgccaaggg cctcagggtg gtgccggccc
      421 gcaattccca catcaccctc gcctttctcg gcgagcgtga cgaattcgag atcgaggagg
      481 tcgcgggtgc cctgctcgag acggccgtcc cggcgaccgg cctggcgctc ggtgccccgg
      541 cctggttgcc gaagcggcgg ccccgcagcc tggtcctgga catccacgat gaccggggcg
      601 aactgaagga gcttcaggcc ggaatggccg gggccctgaa atcggccctg ggctggaagg
      661 aggaccggcc ctaccgcccg cacctcacct gcgccaggac cggccgcggc ttcgatccct
      721 ctcatctgcg ggtgccggtc agcccctcga tcgccttcag cggtcagtcg gtgacactct
      781 acgcctccca cttggggccc gagggcgccg aatacgaagt gatcgaacgg gtcgatcagt
      841 agaagccgac ccggcccggg ccggaccggc cagcctgacg ccctacccac cttccgggtg
      901 acctttgggg caccccggca gcccaaacgc aacccggagt aatcggtcag gcctacgctt
      961 ccgtctcggc gacggttggc tgagaccggc atctgagacc ggcatgtgtg tgaatcgttg
     1021 ctcagtttgc acacttttga gctttttcgt ggcaggctcg acggatgtct gaccgagcgg
     1081 aaatcgaagc ctcactccag aggatcgcgg ggaagcaatt cgggcttgtg acccgaacgc
     1141 agatgctcgg agcgagcctt tccaaggcga tgatcaagca tcgtcaagac cagaatcgcc
     1201 ttctgccggc gggcagggcc gtctttgcgg ttggccgccg cgccgagggt cgactggctg
     1261 cctgcatggc cgccactctt gtggccgggg gcggtgcagt cctctccggc cgggctgctg
     1321 ccgatgtttg ggacattcga aaagaccgcg ggaaccttga ggttaaacgg cgcaacagcc
     1381 acagatctgg cgagttcgtt ctggatcgtc gaggtgttgc cgaggcgaga ccggtgaggg
     1441 ttcgcaggag tcgatacttc aatcccgctc aggtcacccg caaatacgga atcccgatcc
     1501 tgcgaccttc ctggatcctg ctggatctag cccgggaact ccccgacgat gagttcggac
     1561 atgccttcaa ggaagcggat cgcctgggct tgctcaatga agaagagttg agagcttgcc
     1621 ttgagttggg ttccggactc gaaggaatca gaaagttcca gaatctcgtc gctcggcgac
     1681 acccggacct taaggatgtc cggactcttc tcgagaccct attcctcgaa ctctgcgcca
     1741 gatacggcat cgagactcca agagtgaacc gtcgcaaggg ccgatacttc cctgacttca
     1801 gttgggatga catcggcttg atcgttgagg tcgatgggta cgaagggcac gccggacggt
     1861 tcgctttcct cgatgacgcg gcgcgggaaa acgacctccg tcggctcggc taccaggtga
     1921 tcaggttcac ctgggaggag atcaccgagc accccgaact cgtcgcgagt ttggtcattc
     1981 gggagatggc ccggtgccgg gcgctggccc gggtcgcttg atcacagttg gaaggattcc
     2041 gggtgacttt tggggcgcca cagccgacca aacttcaccc agagcggatc acgtacgcgt
     2101 ttcgcctcca tttgggtgac tttggggcac cacagccaac caaacgtcac ccggaagttg
     2161 aatgaagcct ccgagagaca tttcagaggg aacgatgccg aatcagtatt gggaacgggg
     2221 cgacggctac ttgacccgga aggcgccgcg catcaggggg tggatgcggc agaagaaggt
     2281 gtaggtgccg cggggcaggt cggccggggt cttccatgag gtccgctcga ccgtgggcgc
     2341 tccgccgact ccgagctggc cggagtcgaa ggtgaacttg ccgtccggga tcgggtaggc
     2401 gataccggtc gacttgttac agggcgtctt gcaggaggtc agcgagtgcc agatctcgtc
     2461 gtttatgtcg tcctggttga gctggaaggt caggctcttg ccttcatcca ccaccggcgg
     2521 gcgaccggcc gcgccgggca gccggaagtc accgccgttg aaggagaagc cgctgatcgt
     2581 gaacgggttc ccgtcggggt ttgctccgtc cggcagcctg gtcgggtcgg gtccgaccac
     2641 cggcagcttg ccgccgtgat cgttgttctc cgcgtaatga ccgtggatca gctggccctt
     2701 gtagtcgacc ttcttccggt acgggttggt gccgtgctcg ccctcggcca tgtagacgac
     2761 catgatcccc atcgactcgg gccaggaggc cagcttcgtc tcgtaggttg agttcatcga
     2821 gaggacgtcg cccttcttga cctcgggccg ccagtcgtcc ctggtgccga ccatcgccat
     2881 gtcccaggaa acgggaccgg ccggctcgaa gtaatgcgcc tgcgaacgga agaggtggac
     2941 cttgctgccc ttgacgctcg gcatcgaagc cttgcaggat ttgtacttct tcagcttcgc
     3001 cccgtaacga cggatgttcc tgcggttcgg cggtctggcc gctttgggct gcacgcactt
     3061 cggaccggcg tacctggcgc cggggcgctg caggtagagg tcggtgtaga ggccaccggt
     3121 gtggacgtgg ccggcggtgg cgagcaggac gccgtcgcga tcaacggtcc aggtgttttt
     3181 ggctacgccg ttcgggtagg gattgtcggc gtcatccggg taggtgaact tgccgtcctt
     3241 gccgccgctg cccttccaga tgtcgaagac cgggtagacg cttccgttgt ccacgtccat
     3301 ccagatcggg gtgaccggct tgatcccgtc ggcggccggt gaatcagccg ggatgaagtc
     3361 gatcgtgtag gtcgcgtaga gcgtcatcgc ttccggcgtc aggttgtgga tcatgtgatt
     3421 gaggagccag acgtcggatg ccttgtactg gtagccgtaa ccgtcgggga accgggtgat
     3481 cgtcttttcc tcaccggttg cgaagaagcg ttcctgacca cccgaggtcg cgtcctggcg
     3541 gctgaaattg agccacacac cgtggtggaa catgaccttg ctcgaaagcg ggatcgagcc
     3601 gtcctcgttg accatgttcg gcttgatccg ggtgatccag ccgtccaccg agggcttctc
     3661 gatcccggtc agcggcttga aggcgatcct gttctggccg ggcgtaacgg taagcgggcc
     3721 gatccggtag gtgattcgtt cggtgccgtc atcgagacgt ttggtggtgc tgtgaggaag
     3781 cagtgccgaa gccgactggg gcaggaccag aatcgcggcg agcatcagaa atgcgacgaa
     3841 gaacctgcgg aagctcatct ggctacttga cctttcgtga ggcgctcgcc gacttgccgt
     3901 agggcacggc ggcgctcagt ttgatctgga cagacttcgg cctcgcgatt gccttgcggc
     3961 cggccttggt caacggcagg ttgaccgtct tcgactgtcc tgcggtgaaa tcgagggcgc
     4021 ggctggtacc gagcgtgacc tttcgtgagg cgctcgccga cttgccgtag gg
//
LOCUS       DAOQFI010000065         4065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_9718_length_4065_cov_3.996259, whole genome shotgun sequence.
ACCESSION   DAOQFI010000065 DAOQFI010000000
VERSION     DAOQFI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9718_length_4065_cov_3.996259"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLE93_01965"
     CDS             <1..516
                     /locus_tag="PLE93_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85844.1"
                     /translation="HACKRSFPNPLSAIGHFSGIGRLPNAPEALSRYRATRLGEFEAG
                     DLDALNGAFMLVRREALLDVGPLDEGYWLYMEDLDWCRRFHQSGWRVFYEPAGTALHV
                     KGGSSPGRRRPRQEIAFHRGMGRFYRRFDAPENPGVVNLAVYLGIGAKLAVSLLITAA
                     GCFRNRSACGR"
     gene            complement(488..1069)
                     /locus_tag="PLE93_01970"
     CDS             complement(488..1069)
                     /locus_tag="PLE93_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85845.1"
                     /translation="MVLAFGVAPGTVLASSATDVYTEEPPPPTPGKNDNNGNNTNNGN
                     GGTSGNQNSGANGESGSSGSSGSSGGDSDSSGYGSGYSSGSGDGTSTPSKQYSNGQYN
                     PATDSDESGATGATGSSDDSNPDDSTITATPASGDGGDGGGGSALPLIIGLLIGVPLI
                     AGGGYYAWTRYRVRDEDRDRLKTALSDRKPTGF"
     gene            1201..3051
                     /locus_tag="PLE93_01975"
     CDS             1201..3051
                     /locus_tag="PLE93_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNH85846.1"
                     /translation="MALSGGGYDLLARHLAGILAWVLVAVLLLGPWPDRLRPGRSLAL
                     IGGLLLLLAILSALSSIWSSSVSVSLAEAERVIGYLGFFTASYLTMRTPKQREWFARG
                     IAAGLAVIVLLALGDRLLPGGEPGVVPGIDRLSFPLGYWNANGVCAATALVLFTWFAA
                     VTRSKGWRAGSVAMAMLSGTALYLTYSRGGLLLAVIAAILLCLLTPRRLRVLATLAVV
                     VAGTVPILLTVNLYPEISGDSFGDPSLGESLIVILVALGASLLAAAVLEGLFGLGRRQ
                     PEVTERAREISRDRRVLIGIIGAGATLLIVFSFAFGQNVWDQFTDDDVPVPTDARSRF
                     TELSGSWRYEFNRVALDTFSDNPVLGTGAGTYPIEWSRRREVPVITQDAHSFYLENLA
                     ELGIPGGLISISLVISLLTLAAWAFRRRLGREAPVILALTAGLLFSLAFDWFWKLGVT
                     AALLMCLAAWIASAEAVDPERPQTGAGTGFKVAGLLAAWVSIVILAVPALADHYVEAS
                     ADSVRAGNVDRAYDQAKAASRLEPFSADPHMQLGTIAEGRGQYALALAEYNKAIELEP
                     ENWHALVLRFGLNYHAGKLQAARADYARLREINPLYFSEYSFREIQELAS"
     gene            3185..>4065
                     /locus_tag="PLE93_01980"
     CDS             3185..>4065
                     /locus_tag="PLE93_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85847.1"
                     /translation="MLLAALLISFLGLTTFVSKADAARSFQTGIFDGSVYDHGDDEAF
                     DRTADSGATLIRSNLYWTYMVANGDSADRPGSEGQPFNATDPGSPYYNWGVYDRMVRK
                     SAARGLQMVFSIVSPPRWARISSCADSGVCSPRPADYADFATAAARRYDGTFDPGDGN
                     GVLPRVRFWQAANEPNFYLFYKPLLKPNGAPAAPYEYRKILNAFYGAIHAVDNSNVVI
                     AAGLAPNGKKGKSISPLDFTRRALCMTGSYKHPRPIAGCKFKVKADAWAVHPYTTGGP
                     THVPANGNDMSVAALPRL"
BASE COUNT          624 a         1390 c         1254 g          797 t
ORIGIN      
        1 cacgcatgca aaagatcttt cccgaacccg ctttcggcga tcggtcactt cagcgggatt
       61 ggtcgtctgc cgaatgcgcc ggaggccctg tccaggtacc gggcgacgcg gttgggagaa
      121 tttgaagccg gcgacctcga tgccttaaat ggagccttca tgctggtccg tcgagaggca
      181 ctgctcgatg ttggcccact cgacgagggt tattggctct acatggaaga cctcgattgg
      241 tgcagacggt tccatcagag cggatggaga gtcttctatg aaccggcagg gacggccctt
      301 cacgtcaagg ggggatcaag cccggggcgg cgaaggccac ggcaggagat cgcctttcat
      361 cgcggcatgg gccgttttta ccggcgcttc gacgcgccgg agaacccggg cgtggtcaat
      421 ctcgcggtct atctggggat cggcgcgaag ctggcggtca gcctgctgat cacggctgcc
      481 ggctgcttca gaaaccggtc ggcttgcggt cgctgagggc ggtcttgagg cggtcccggt
      541 cctcgtcacg cacccggtag cgggtccagg cgtagtagcc gccgccggcg atcagcggta
      601 cgccgatcag caggccgatg atcaggggca gggccgaacc gccgccgccg tcgccgccat
      661 cccccgaggc cggggtcgcg gtgatcgtcg aatcatccgg gttcgaatcg tcgctcgaac
      721 cggtcgctcc ggtggcaccc gactcgtcgg aatccgtggc cgggttgtat tgcccgttgc
      781 tgtactgctt gctcggggtc gaggtgccat ccccggatcc ggagctgtaa ccggagccgt
      841 agccgctcga atccgaatct ccgccgctgc tgccactgct gccgctgctg ccggattcac
      901 catttgcgcc gctgttctgg ttgccgcttg tgccgccgtt gccgttgttg gtgttgttcc
      961 cgttgttgtc gttcttgccg ggggtgggcg gtggcggctc ttccgtgtag acgtccgtag
     1021 ctgacgaagc aagtaccgtc cccggtgcca caccgaatgc caggaccatc agccccaaga
     1081 tcgatgccgc aaggcagagc aggcgccgcg cggaagcgga acttgtcgca ggagcgttca
     1141 tacgcacgga agatagccga tctgatcgcg tttctggttc cattcctgct cctcgtcggt
     1201 ttggcactga gtggcggcgg ttatgacctt ctggctcgtc acctggctgg aatcctcgcc
     1261 tgggtcctcg tcgccgtgct gctgctcgga ccctggccgg accggctccg cccgggccgg
     1321 tcgctcgccc tgattggcgg tctgcttctt ctgctggcaa ttctttcagc gctttcgtcg
     1381 atctggtcga gttcggtttc ggtcagcctg gccgaagccg agagagtcat tggctatctc
     1441 ggtttcttta cagccagtta cctcaccatg agaaccccga aacagcgcga atggttcgcc
     1501 cggggcatcg cggccggcct ggcggtgatc gtcctgctgg cgctcggtga ccgcttgctg
     1561 cccggcgggg agcccggagt cgttcccgga atcgaccgtc tcagcttccc gctcggttac
     1621 tggaacgcga acggcgtctg cgccgccacg gcccttgtcc tcttcacctg gtttgccgcg
     1681 gtgaccaggt caaaagggtg gcgggccgga tccgtggcca tggccatgct ctccggaacc
     1741 gcgctctatc tcacctactc ccggggcggc ctgctgctcg ccgtgatcgc ggccatcctg
     1801 ctttgcctgc tcaccccgcg ccggctccgg gtcctggcca ccctggcggt cgtggtcgcc
     1861 ggaacggtcc cgatcctgct cacggtcaat ctctatccgg agatctccgg cgacagcttc
     1921 ggtgacccct ccctcggaga gagcctgatc gtgatccttg tcgccctcgg ggcctcactg
     1981 ctcgcggcag cagtcctcga aggtctgttc gggctcggcc gccgtcagcc ggaggtgaca
     2041 gagcgagccc gcgagatttc ccgggatcga agagtgctca tcgggatcat cggggccgga
     2101 gccaccctgc tgatcgtctt ttcgttcgcg ttcggccaga acgtctggga tcagttcacc
     2161 gacgatgacg ttcccgtccc gaccgatgcc cgctcacgct tcacggaact ttcgggatcc
     2221 tggcgatacg agttcaaccg ggttgccctg gacaccttct ccgacaaccc ggtcctcgga
     2281 accggtgccg gcacctaccc gatcgaatgg tcccgccgcc gcgaggtgcc ggtgatcacc
     2341 caggacgccc actccttcta tctggagaac ctggccgagc tcggcatccc gggcgggctg
     2401 atctcgatct ccctggtcat ttcacttctg accctggcgg cgtgggcctt tcgccggcgt
     2461 ctgggaaggg aagctccggt gatcctcgcc ctgaccgccg gactgctctt ctccctggcc
     2521 ttcgactggt tctggaaact cggggtcacc gcggccctgc tgatgtgcct cgccgcctgg
     2581 atcgcctcgg ccgaggcggt ggacccggag cgcccgcaga ccggtgccgg taccggcttc
     2641 aaggtcgccg gcctgctcgc cgcgtgggtc tcgatcgtga tcctggccgt tccggcactg
     2701 gccgaccact acgttgaggc aagcgccgat tcggtccggg caggcaacgt cgaccgcgca
     2761 tacgaccagg cgaaagccgc ctcaaggctg gaacccttct cggccgaccc ccacatgcag
     2821 ctgggcacga tcgccgaagg gcggggccag tatgccctgg cgcttgccga gtacaacaag
     2881 gcgatcgaac ttgagccgga gaactggcat gccctggtcc tgcggttcgg actcaactac
     2941 cacgcaggga agctgcaggc ggcccgggcc gactacgcgc gcctgcgaga aatcaatccg
     3001 ctctacttct ccgagtactc attcagggag attcaggagc tggcctcctg acccccaact
     3061 cgccggttcg ctactttctg cctttggcga tgttcaagta cacaagcgcc acccgagact
     3121 tcgcgccctc gagcgaagtt tgaccctccg aaaggatccc ttgcacgcaa tctcgaatcg
     3181 cggaatgctg ctcgcagcac tgctgatcag ctttctcggc ctgaccacct ttgtctcaaa
     3241 ggccgacgca gcgcgctctt tccagaccgg gatctttgat ggcagcgtgt atgaccacgg
     3301 tgacgacgag gccttcgatc gcaccgccga cagcggcgca acgctgatca ggagcaacct
     3361 ttactggacc tacatggtcg ccaacggcga ttcggccgat cgcccgggat ccgagggcca
     3421 gcctttcaac gcgaccgacc cgggcagccc ctattacaac tggggggtct atgaccgcat
     3481 ggttcgcaaa tctgccgccc gcgggctcca gatggtcttc tcgatcgtca gccctccgcg
     3541 ctgggcacgg atttcgtcct gtgccgattc cggtgtctgc tctccccggc ccgccgatta
     3601 cgcggacttc gcgaccgcgg ctgccaggcg ttacgacgga accttcgacc ccggtgacgg
     3661 caacggtgtc ctgccccgcg tgaggttctg gcaggccgcc aacgagccca acttctacct
     3721 cttctacaag ccgctgctga agcccaacgg ggcaccggct gctccctacg agtaccgcaa
     3781 gatcctcaac gccttctacg gcgcgatcca cgcggttgac aacagcaacg tcgtaatcgc
     3841 tgccgggctc gccccgaacg gcaaaaaggg caagtcgatc tcgcccctcg atttcacccg
     3901 tcgcgccctc tgcatgaccg gctcctacaa gcatccccgc ccgatcgccg gctgcaagtt
     3961 caaggtgaag gccgacgcct gggcggttca cccgtacacg accggcggtc cgacccacgt
     4021 tcccgccaac ggcaacgaca tgtcggttgc cgccctgccc cgcct
//
LOCUS       DAOQFI010000066         4043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_9798_length_4043_cov_3.680040, whole genome shotgun sequence.
ACCESSION   DAOQFI010000066 DAOQFI010000000
VERSION     DAOQFI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9798_length_4043_cov_3.680040"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /gene="thiE"
                     /locus_tag="PLE93_01985"
     CDS             complement(<1..636)
                     /gene="thiE"
                     /locus_tag="PLE93_01985"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNH85848.1"
                     /translation="MSFEPADGAGGLRRERLRTARLYFVCDAAPNGNDPEPLLKAALD
                     GGVDIVQLREKEADTRVIERAADTFRRVCDTYGVPFILNDDPELAMSVRADGVHIGQD
                     DISPAEAREIIGPNHLLGLSTHSEEQIAAAHQTVRDGTPIDHISVGPINETPTKPGRP
                     ATGLELIRHAASTTTIPFFAIGGIDPDNVLEVLQAGARRICVVRAIHDAADP"
     regulatory      677..790
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            799..1947
                     /gene="thiO"
                     /locus_tag="PLE93_01990"
     CDS             799..1947
                     /gene="thiO"
                     /locus_tag="PLE93_01990"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNH85849.1"
                     /translation="MNRAEPREESDVIVVGAGLIGLACAWELAGSGVSVTVFERDEPG
                     CGATRVAAGMLAPIGELDFGEPDLLALNLRSRRLYREFTGEVERATGLDTGYRHTGAL
                     HVALDRDEAGELKREMELQRSHGLESKWLGPIAARDLEPGVSPSLAGAVLVEEDAVVD
                     PRALVEALRAGLAQRGVEIVSAEVTGLERDSAGRVRGVGTSIGSFAAATVVAAPGAVA
                     GVAGWLPEEVRPPVRPVKGQTVELRGPSEDPVCERILGSERVYIAPRPDGRVILGATS
                     EEMGFDTRVTGGGVHELLREAYRLLPDVAEMEFVGAVAGLRPGTPDNLPIIGRTGIEG
                     LILATGHYRNGILLGPVTAEAVAALVAGDQPEWLEAASPGRFTKQEVG"
     gene            1947..2945
                     /gene="thiS"
                     /locus_tag="PLE93_01995"
     CDS             1947..2945
                     /gene="thiS"
                     /locus_tag="PLE93_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNH85850.1"
                     /translation="MRIELNGTPTEVAEGLTITEVIGLVAGERIPEDRRGVAVALRGE
                     VVPRSEWDSTVPAEGDPVEVVAAIQGGSGEADSGQWELGGRTWTSRLIAGSGGFRSLE
                     AMESALALSGTEIITVALRRLDPSAQGSVMDVIEKLGLFILPNTAGCYTARDAVRTAK
                     LAREAFGTEWVKLEVIGDDRTLLPDAPELLRAAEELVGDGFIVLPYTNDDPILAARLE
                     AAGCAAVMPLGGPIGSGAGIRNPYNISIIVENAKVPVILDAGIGTASDATLAMELGCD
                     AVLLASAVSRAEKPDLMAVAMKHAVEAGRAARQAGRIPKRLYAEASTPEEGLPGLG"
     gene            3002..3778
                     /locus_tag="PLE93_02000"
     CDS             3002..3778
                     /locus_tag="PLE93_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNH85851.1"
                     /translation="MNVDVTTRGSVDPEAVELAQEKLTELESAVGRPLSHVRVVLRQE
                     KESIPNAARAEGEAQVGKTIIRGHVAADSMSHAVHELADRLQQQIRRHLDRVTSLKRQ
                     PVETPDGKWDHGTWVPARPAQSLLAPGEREVIRRKVLSLVPLKASEAAELMRELDHGF
                     YLFHDSDTEADSVIYLRDDGQLAVIAAQGVAAPADGEDSSGIQRETSHYSSALTLEDA
                     VSQMDELNHRFMFFTDAATDRAAIIYLRYDGNYGLIEQEA"
     gene            complement(3809..3882)
                     /locus_tag="PLE93_02005"
     tRNA            complement(3809..3882)
                     /locus_tag="PLE93_02005"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3846..3848),aa:Lys,seq:ttt)
BASE COUNT          664 a         1298 c         1446 g          635 t
ORIGIN      
        1 cgggtcggcc gcatcgtgga ttgcccggac cacgcagatg cgacgcgccc cggcctgaag
       61 cacctcgagc acgttgtcgg gatcgattcc gccgatcgcg aagaagggga tcgtggtggt
      121 ggaggcggca tgccggatca gctcgagccc ggtcgctggc cggccgggct tggtcggggt
      181 ttcgttgatc gggccgacgc tgatgtggtc gatcggagtg ccgtccctga cggtctggtg
      241 ggccgccgcg atctgctcct cggagtgggt cgaaagtccg agcaggtggt tgggcccgat
      301 gatctcgcgg gcctcggccg gggatatgtc gtcctggccg atgtggactc cgtcggcccg
      361 gaccgacatc gccagttcgg ggtcgtcgtt gaggatgaaa gggaccccgt aggtgtcgca
      421 gacccgccgg aaggtgtcgg ccgcccgttc gatcaccctg gtgtcggctt ccttctcgcg
      481 cagctgcacg atgtccacgc cgccgtcgag cgccgccttc agcagcggct ccggatcgtt
      541 tccgttcggg gcggcgtcac agacgaagta gagccgggca gtccgcaggc gttcccgccg
      601 aagtccgccg gcaccgtcag ctggttcgaa gctcatgggc tgaggataga ggcagcaagc
      661 cggggaccgc tagcatgcgt ttacctgcgg gagcccgatg tcccgggctg agagggtggc
      721 gacgaagcca ccgaccgcct gaacctgatc cgggtaattc cggcggcagg gatgggcagc
      781 ggttccggtg ggaagcaaat gaaccgagct gagccgagag aagaaagcga cgtgatcgtg
      841 gtcggggccg gcctgatcgg actcgcctgt gcctgggagc tggccggttc cggggtttcg
      901 gtcacggtgt tcgaaagaga cgaaccgggc tgcggtgcga cccgggtcgc ggccgggatg
      961 ctcgctccga tcggtgagct cgacttcggc gagcccgacc tgctcgccct gaacttgcgc
     1021 tcccgccggc tctatcggga gttcaccggc gaggtcgagc gggcgaccgg tctcgacacc
     1081 ggctatcgcc acaccggcgc cctgcatgtc gcactcgacc gggacgaggc gggggagctg
     1141 aaacgggaaa tggagctgca gcggtcccac gggcttgaat cgaagtggct cgggccgatc
     1201 gccgcccgcg acctcgagcc cggagtcagc ccctcgctgg ccggcgcggt tctggtcgag
     1261 gaggacgcgg tggtggatcc ccgggcgctg gtcgaagcgc tcagggcagg ccttgcgcag
     1321 aggggcgttg agatcgtgag cgccgaggta accgggctgg aacgggattc ggccggaagg
     1381 gtgaggggag tcgggacttc gatcgggtcc ttcgccgcgg caacggtggt ggccgctccc
     1441 ggtgccgtcg ccggggtcgc cgggtggctg ccggaagaag tccggccccc ggtgcgtccg
     1501 gtcaaggggc agacggtcga gctgcgcggc ccgagcgaag atccggtctg cgagcggatt
     1561 ctcgggtcgg aacgggtcta catcgcgccc cgtcctgacg gcagggtcat cctcggcgcg
     1621 acctccgagg agatgggctt cgacacccgg gtcaccgggg gcggggttca cgaactgctg
     1681 cgtgaggcct atcggctgct acccgacgtg gcggagatgg aattcgtcgg cgcggtcgcc
     1741 gggctgaggc cgggcacccc ggacaacctg ccgataatcg gccggaccgg gatcgagggc
     1801 ctgatcctcg cgaccggcca ctaccgcaac gggatcctgc tcggcccggt gacggcggag
     1861 gcagtcgccg ccctggttgc cggtgatcaa cccgagtggc tggaggcggc cagtccgggc
     1921 cggttcacaa aacaggaggt gggttgatgc gaatcgagtt gaacggaacc ccgaccgagg
     1981 ttgccgaggg gctgacgatc accgaagtga tcgggctggt tgcgggggaa cggatccccg
     2041 aagaccggcg cggagtcgcg gtcgcgctcc ggggggaggt cgtcccgcgc tcggagtggg
     2101 actcgaccgt tccggccgaa ggcgacccgg ttgaggtcgt cgccgcgatc cagggcggct
     2161 ccggcgaagc ggattcggga cagtgggaac tcggtggccg gacctggacc tcgagactga
     2221 tcgccggcag tggcggcttc cgctcgctgg aagcgatgga aagcgccctg gcactttcgg
     2281 gaacggaaat catcaccgtc gccctgcggc ggcttgatcc ttcggcccag ggttcggtca
     2341 tggacgtgat cgagaagctc gggctgttca tcctgccgaa cacggccggc tgttacaccg
     2401 cccgcgacgc ggtccgcacg gcgaagctcg cgcgggaggc cttcggcacc gagtgggtca
     2461 aactcgaggt gatcggtgac gatcgcaccc tgcttcccga cgctcccgaa ctgctcaggg
     2521 cggccgagga gctggtcggt gacggcttca tcgtgctgcc ttacaccaac gacgacccga
     2581 tcctcgccgc ccggctggag gcggccggct gcgccgcggt gatgccgctg ggcgggccga
     2641 tcggctcggg cgcggggatt cgcaatccgt acaacatctc gatcatcgtc gagaacgcaa
     2701 aggtgccggt gatcctcgac gccgggatcg gcaccgcctc cgacgccacc ctggcgatgg
     2761 agctcggatg cgacgcggtg ctcctggcca gcgcggtttc ccgggccgag aagccggacc
     2821 tgatggcggt cgccatgaaa cacgcggttg aagcgggccg cgccgcccgc caggccggcc
     2881 ggatcccgaa gcgcctttac gccgaagcca gcacccccga agaaggcctc cccggcctcg
     2941 gctaagtccc ccgcatgaag gtgcccctcg gggattcgaa cgcgtcacct atgatctggc
     3001 gatgaacgtc gacgttacga cccgtggttc ggttgatccc gaagcagtcg agctggccca
     3061 ggagaaactc accgaactcg agtccgccgt cggccgtccg ctgagtcacg tccgggtggt
     3121 gctgcggcag gagaaggaga gcatcccgaa cgccgcccgg gccgagggcg aagcgcaggt
     3181 gggcaaaacg atcattcggg gccacgtggc cgcggacagc atgtcccacg cggttcacga
     3241 gctggccgac cggcttcagc agcagatccg gcggcacctc gatcgggtca ccagcctcaa
     3301 gcgccagccg gtcgagaccc cggacggcaa gtgggatcac ggaacatggg taccggcccg
     3361 gccggctcag tcattgcttg ctcccggaga gcgggaagtc atccggcgca aggtcctttc
     3421 actcgtgccg ctgaaggcct ccgaggcggc ggaactgatg agggagctgg accatggctt
     3481 ctacctcttc cacgactcgg ataccgaggc cgattcggtc atctacctcc gggacgatgg
     3541 ccaactcgcg gtgatcgccg cacagggcgt cgccgccccc gcggacgggg aagactcatc
     3601 cggaatccag cgtgagacca gccattactc gtcggccctg acccttgagg atgcggtgag
     3661 ccagatggac gaactgaacc accgcttcat gttcttcacc gatgccgcca cagaccgcgc
     3721 cgcgatcatc tacctgcgct acgacggcaa ctacggcctg atcgaacaag aggcctgaga
     3781 ttcgcggcgc tcgccgcgcc gcagcaaatg ccccctctgg gattcgaacc caggactcac
     3841 aggttaaaag cctgcagctc taaccaactg agctaagggg gcccgcggag cttaactgca
     3901 agtcggcgag cttcgctccg cctccaagtc aaacccgccg gcccacagaa gcaactgatg
     3961 gatgggccgg ctctgtccgg tggcgatact gactcgcctc aattgggtac cttgcgagtg
     4021 tgtccagaat catccgcgtt ttc
//
LOCUS       DAOQFI010000067         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10055_length_3989_cov_3.521607, whole genome shotgun sequence.
ACCESSION   DAOQFI010000067 DAOQFI010000000
VERSION     DAOQFI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10055_length_3989_cov_3.521607"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLE93_02010"
     CDS             <1..618
                     /locus_tag="PLE93_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNH85852.1"
                     /translation="QAIAAENPDASLPDQDIVVVHRSDESGTTENFTDYLHQAAPKDW
                     TDEPDGVWPLQGGEAANGTSGVISAVKSGSGTIGYADLSQVGDLGTVNVKVGSDFVAP
                     SPEAAVKIFDESKRIGGEGKYIFAYELNRTSTAQGLYPISLVSYEMACTTYDSADTAK
                     LVKAFYSYIISDEGQQAAASNAGSAPIPASLSKQITPAVEAIQTS"
     gene            720..1673
                     /gene="pstC"
                     /locus_tag="PLE93_02015"
     CDS             720..1673
                     /gene="pstC"
                     /locus_tag="PLE93_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNH85853.1"
                     /translation="MNAGTVSNSETPPSGRAHRRFGDRAFSGLATAAGLAILVVLAGV
                     AAFLVSEAWPAITAPVADLPDGKSLLHYIWPLVFGTVLSSLIAILIAIPMAIAVALFT
                     SHLAPKPIAMSVSYLTDLLAAIPSIIYGIWGIAVLGPASVPVTQWLSDHFGFIPFFDG
                     PASTTGRTMLVAGVVLALMILPIISAVAREVFQQTPKGLQESALALGATRWEMIRMVV
                     LPFGRSAVVAGSMLGLGRALGETMAVTIILSVSGVITFDLISSENPSTIAANIALQFP
                     ESSGIDVNVLIATGLVLFAITLAVNMAARAVIGRREAAGGR"
     gene            1684..2718
                     /gene="pstA"
                     /locus_tag="PLE93_02020"
     CDS             1684..2718
                     /gene="pstA"
                     /locus_tag="PLE93_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNH85854.1"
                     /translation="MPLGEGSGVLPRRAGLLVFGISAVASALLCLVLGFSPFVWLIFT
                     GLFSLVTITVWSLAVEGRRRALDRVMTVVIVEIFVLALVPLISLVYTVVSEGLARFDV
                     QFLTEDMRGVVGEGGGAFHAAMGTFIITAFATVAAVPVGVLAAIYLVEYGKGRLKRSI
                     TFFVDVMMGIPSIVAGLFAFALFALFFGPGIRLGIMGSVALMVLMIPIVIRSAEEMLR
                     VVPNSLREASYALGVPKWLTVLRVVLPTALAGIVTGVMLAVARVIGETAPLLVTTGSI
                     DTTNLDPFDGRMANLPVYVYEQYKFPGVPPEAFIERAWAGALLLILIVMVLFLAARLI
                     SRRYGVEIRR"
     gene            2829..3608
                     /gene="pstB"
                     /locus_tag="PLE93_02025"
     CDS             2829..3608
                     /gene="pstB"
                     /locus_tag="PLE93_02025"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNH85855.1"
                     /translation="MAKRIDAEDLNIYYGSFLAVKDCTVSIHPNKVTALIGSSGCGKS
                     TFLRSLNRMHELIDGARVEGRVLLDGMDIYSPSMDPVLVRQQIGMVFQQPNPFPTMSI
                     YENTAAGLRLNRRRLKKTEIDGIVERSLRGAHLWDEVKDRLEKPASGLSGGQQQRLCI
                     ARATAIEPEVLLMDEPCSALDPIATLAIEDLINELKKRYTIVIVTHNMQQAARASDYT
                     GFFNIEKSGDPGVLVEIDETEKIFSNPSLQETEDYVSGRFG"
     gene            3688..>3989
                     /locus_tag="PLE93_02030"
     CDS             3688..>3989
                     /locus_tag="PLE93_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85856.1"
                     /translation="MLCAAIDIGSNTTRILVAEPDEGKLKKVMEQRAYTRIGQARLDD
                     GTIPAEKVAEVAEVVDTQVKLARELGAGTFRAVATAALRDAPNGKEAAAEIEAVAG"
BASE COUNT          664 a         1350 c         1263 g          712 t
ORIGIN      
        1 caggcgatcg cggcggagaa cccggacgcg agtctgcctg accaggacat cgtggtcgtc
       61 catcgctcgg atgagtcggg cacgaccgag aacttcaccg actacctgca ccaggcggct
      121 ccgaaggact ggaccgacga gcccgacggg gtctggccgc tccagggcgg cgaggctgcc
      181 aacggcactt ccggcgtgat cagcgccgtc aagtccggca gcggcacgat cggttacgcc
      241 gatctgagcc aggtcggtga cctgggcacc gtcaacgtca aggtcggcag cgacttcgtg
      301 gctccctcac ccgaagcagc tgtgaagatc ttcgacgagt cgaagcggat cggtggcgaa
      361 ggcaagtaca tcttcgcgta cgagctcaac cgcacctcga ccgcccaggg tctctacccg
      421 atctcgctgg tgtcatacga gatggcctgc accacctacg actcggctga cacggccaag
      481 ctcgtcaagg ccttctacag ctacatcatc agcgacgaag gccagcaggc tgcggcctcg
      541 aacgccgggt cggctccgat cccggcatcc ctcagcaagc agatcactcc cgccgtggag
      601 gcgatccaga ccagctgagc tgaagcaagc cggcagcgag aaccccgcgg cggaggcaca
      661 ggccgccgcg gggtgatcgc agtcaggctc accagccgtt tttcgggcag gagatttcat
      721 tgaacgcggg caccgtctca aacagcgaaa caccaccttc aggcagggct caccgccggt
      781 tcggtgaccg tgccttttcc ggtcttgcca ctgcggccgg cctggcgatc ctggtcgtgc
      841 tggccggggt cgcggccttt ctggtcagcg aggcctggcc cgcgatcacc gctccggtcg
      901 ccgaccttcc cgacggaaag tccctcctcc actacatctg gccactcgtc ttcggcaccg
      961 ttctttcctc gctcatcgcg atcctgatcg cgatccccat ggcgatcgcg gtggcgctct
     1021 tcaccagtca cctcgccccg aagccgatcg cgatgtcggt ctcctacctg accgacctgc
     1081 tggccgcgat ccccagcatc atctacggca tctgggggat cgccgtgctc gggccggcgt
     1141 cggtgccggt cacccagtgg ctttccgacc acttcggctt cattcccttc ttcgacggcc
     1201 ccgcctcgac caccggacgc accatgctgg tcgccggggt ggtgctggcc ctgatgatcc
     1261 tgccgatcat cagtgcggtc gcccgcgagg tcttccagca gaccccgaaa gggctccagg
     1321 aaagcgccct cgccctcggc gcgacccgct gggagatgat ccgcatggtc gtgctgccct
     1381 tcggccgctc ggccgtggtc gccggctcga tgctcggtct cggccgggcg ctcggcgaga
     1441 cgatggccgt gacgatcatc ctctcggtct ccggggtgat caccttcgac ctgatcagct
     1501 ccgagaaccc ttcgacgatc gccgccaaca tcgcgctcca gttcccggag tcatccggga
     1561 tcgacgtcaa cgtcctgatc gccaccggcc tcgtgctatt cgcgatcacc ctggcggtca
     1621 acatggcggc tcgggccgtg atcggcagaa gggaggccgc cggtggccgg tagtgccgta
     1681 tcgatgcccc tgggtgaagg ctccggggtc ctgccccgcc gggccggttt gctcgtcttc
     1741 gggatctcgg ccgtcgcctc ggcgctgctc tgcctggttc tcggcttcag cccgttcgtc
     1801 tggctgatct tcaccgggct cttcagcctg gtcacgatca ccgtctggtc gctggcggtc
     1861 gaaggtcgtc gccgggcact cgaccgggtc atgaccgtgg tcatcgtcga gatcttcgtg
     1921 ctcgccctgg tgccgctgat ttcactcgtt tacacggtgg tcagcgaagg gctggcccgc
     1981 ttcgacgtcc agtttctgac cgaggacatg cggggtgtgg tcggtgaagg cggcggcgcc
     2041 tttcacgccg cgatgggcac cttcatcatc actgccttcg ccaccgtcgc ggccgtgccg
     2101 gttggcgtgc tggccgcgat ctacctggtc gagtacggca aggggcgcct caagcgttca
     2161 atcacctttt tcgtcgacgt gatgatgggc atcccctcga tcgtcgccgg cctcttcgcc
     2221 ttcgccctgt tcgcgctgtt cttcggcccc ggcatccggc tcggaatcat gggctcggtc
     2281 gccctgatgg tgctgatgat cccgatcgtg atccgctcgg cggaagagat gctgcgggtc
     2341 gttccgaact cgctgcggga agcttcctac gccctcggcg tgcccaagtg gctgaccgtc
     2401 ctgcgggtcg tgctgccgac cgccctggcc gggatcgtca ccggcgtcat gctggcggtg
     2461 gcccgcgtga tcggcgagac cgcaccgctg ctggtcacca ccggatcgat cgacacgacg
     2521 aaccttgacc cgttcgacgg ccggatggcg aacctgccgg tctatgtgta cgagcagtac
     2581 aagtttcccg gcgttccgcc cgaggccttc atcgaaaggg cctgggccgg ggcgctcctt
     2641 ttgatcctga tcgtgatggt gctgttcctt gcggcccggc tcatcagtcg acgctatgga
     2701 gtggagatcc gccgatgaac cagaccgaga ctatgaagga gaaagagatg gttgacgccc
     2761 aggccggacc ggccgacccc atcatggacc ccccggccag cccgagtgcc accgcgaccg
     2821 tggacgccct ggcgaaacgg atcgacgccg aggacctgaa catctactac gggagcttcc
     2881 tcgcggtaaa ggactgcacg gtcagcatcc acccgaacaa ggtgaccgcg ctgatcggct
     2941 cttctggttg cggcaaatcg accttcctca ggtcgctgaa ccgcatgcat gagctgattg
     3001 acggcgcccg ggtcgaaggc cgggtcctgc tcgacgggat ggacatctac tcgccgtcga
     3061 tggatccggt tctggtccgc cagcagatcg gcatggtctt ccagcagccg aacccgtttc
     3121 cgaccatgtc gatctacgag aacacggcgg cgggcctgag actgaaccgc cgcaggctga
     3181 agaagaccga gatcgacggg atcgtcgaac gctcgcttcg cggcgcccac ctctgggacg
     3241 aagtcaagga ccggctcgaa aagccggcca gcgggctctc cggcggccag cagcagcgcc
     3301 tctgcatcgc ccgcgcgacg gccattgaac cggaagtcct gctcatggac gagccctgtt
     3361 cggccctgga tccgatcgct accctggcga tcgaggacct gatcaacgaa ctgaagaagc
     3421 gctacacgat cgtgatcgtc acccacaaca tgcagcaggc ggcccgggcc agcgattaca
     3481 ccgggttctt caacatcgag aaaagcggtg acccgggcgt cctggtcgag atcgacgaaa
     3541 ccgagaagat cttctcgaac ccgtcgttgc aggagaccga ggattacgtc agtggccgtt
     3601 tcggttgagc ttccggtccc gccggagggc cgaaggctcc gaaccggccc gtccgcttcg
     3661 cctgccagaa tcgtttgggt aaagacgatg ctctgcgcag cgatcgacat aggttcgaac
     3721 accacccgga tcctggtcgc cgagcccgat gaaggcaagc tcaagaaggt gatggagcag
     3781 cgggcctaca ccaggatcgg ccaggcccgg ctggatgacg gcacgattcc ggccgaaaag
     3841 gtcgccgaag tcgccgaagt ggtcgacacc caggtgaaac ttgcgcgcga actcggcgcc
     3901 gggactttcc gggcggtcgc cacggcggca ctgagggacg ctccgaacgg gaaggaagcc
     3961 gcggccgaaa tcgaggcggt ggcgggcat
//
LOCUS       DAOQFI010000068         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10056_length_3989_cov_3.120234, whole genome shotgun sequence.
ACCESSION   DAOQFI010000068 DAOQFI010000000
VERSION     DAOQFI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10056_length_3989_cov_3.120234"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLE93_02035"
     CDS             <1..840
                     /locus_tag="PLE93_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNH85857.1"
                     /translation="NDGADSPVALIGRGGGLRLDGIELGNSVTVVGARKATGYGLEVA
                     ASIGREVAAAGLNVISGLALGIDGAVHRGALERGPTVAVLGCGADRPYPASHSRLYRQ
                     VVESGLVISEVPPGADAWRWSFPARNRIMAALSAMTVVVEAAWRSGSLITAEMASDAG
                     RDVGAVPGPVTAGAAAGTNELLANGAALIRGGQDVLDRMLGAGAGQPLFGPAIEDFEV
                     ELLEAVEAGCRTVDEAALMLEREVGEVATALARLEVRGYLRASTIGTWTRTSLATYPS
                     GGE"
     gene            833..1618
                     /gene="thiD"
                     /locus_tag="PLE93_02040"
     CDS             833..1618
                     /gene="thiD"
                     /locus_tag="PLE93_02040"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNH85858.1"
                     /translation="MSEERQIPAVLSIAGSDSGGGAGIQADLKAFAACGVHGMTAITA
                     ITAQNTVGVDLIEAISPEMIIAQIKAVAGDIGVDAVKIGMLGDRPTIEAVASGLDLLG
                     DVPVVLDPVMVSESGANLLAVEAREALVTRILPRATVTTPNIPEARAITGLAEAGQPE
                     LAEVIVGLGVKAAVVTGGHSDEYVDFFFDGERGVEITGEVYPGRSAHGSGCTHSSALA
                     AGLALGLGPLEAARKARELASRAVAGSLDGIGSGPGPVNALGL"
     gene            1709..3397
                     /gene="ilvD"
                     /locus_tag="PLE93_02045"
     CDS             1709..3397
                     /gene="ilvD"
                     /locus_tag="PLE93_02045"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNH85859.1"
                     /translation="MTGNDLPANLPRPRSAAVFDGPDRAAARAYMKGIGLSDEDLSKP
                     TIGIANTWTEAMPCNYGLRDLAAYVKEGVKAAGGTPMEFNTVAVSDGITMGTQGMKSS
                     LVSREMVADSIELMARGYQFDAIIALAACDKTIPGAVMGVARIDIPSIVLYGGSVKPG
                     RYKGKDVSILEVFEAVGAHIAGRITDEELTEIENVASPGYGACGGQFTANTMACAFEA
                     LGISPAFSAMVPAEDKQKTAVAEKVGELILQVLADDLKPSRIITRKSLENAIAAVCAS
                     GGSTNGVLHLLAIAREAGVELDIDVFEEVSRRTPLLADLKPGGRYVATDLYRAGGVPL
                     ILRNLKQGGLLHEDEITVTGRSIGEEVANAEEEPGQDVVLPVDAPLKAQGGLAILRGN
                     ICPEGAVVKVAGTERRHHTGPARVFEREEDCFAAVKRGEIEKGDVIVIRNEGPSGGPG
                     MREMLQVTAALVGEGMGEHVALLTDGRFSGATRGLMIGHVSPEAVKGGPIGAIHDGDE
                     ITVDIDGRRLEVDLTPEQIDERLKGYSPPTPLYDRGVMAKYAATVSSASMGAVT"
     gene            3448..>3989
                     /locus_tag="PLE93_02050"
     CDS             3448..>3989
                     /locus_tag="PLE93_02050"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNH85860.1"
                     /translation="MREWGRAIRAIVGIALAGLLIGIASQPAAAAEQKFTWLCKPGRA
                     NDPCQQSLRTETVSKDETLGVRTPRTLTRLRNKVDCFYVYPTVSFQTGPNADLTEDPQ
                     VDAIAGQQASRFTPGCRMFAPIYRQFTVPAILGSEITEQVNDTAYSGVKAAWNEYLGR
                     YNKGRGIVLIGHSQGTGHLKR"
BASE COUNT          699 a         1271 c         1419 g          600 t
ORIGIN      
        1 aacgacgggg ccgattcgcc ggtggccctg atcggccggg gcggcgggct tcgcctcgac
       61 gggatcgaac tcgggaactc ggtcaccgtg gtcggagccc gcaaggcgac cggctacggg
      121 cttgaggtag cggcgtcgat cggccgcgaa gtggcggccg cggggctgaa cgtgatcagc
      181 gggctggcgc tgggcatcga cggtgccgtt catcgcgggg cgctggagcg gggaccgacc
      241 gtggcggtgc tcggctgcgg ggcagaccgg ccctacccgg cctcacattc gcggctctac
      301 cggcaggtgg tggaaagtgg tctggtgatc tccgaggttc cgcccggggc tgatgcctgg
      361 cgctggagct tcccggcccg gaaccggatc atggcagcgc tctcggcgat gaccgtggtg
      421 gttgaagcgg cctggcgttc cggctcgctg atcacggccg aaatggccag tgatgcgggc
      481 cgggacgtgg gggcggtgcc cggtcccgtg accgccggag ccgcggcagg gaccaacgaa
      541 ctgctcgcca acggggccgc cctgatccgt ggcggtcagg acgtgctcga ccggatgctc
      601 ggggcggggg caggccagcc cctgttcggt ccggcgatcg aagattttga agtcgagttg
      661 ctggaagcgg tggaggccgg ttgtcgaacc gttgatgaag cagccctgat gctggaaaga
      721 gaggtcggcg aggtggccac ggccctggcc cggctggaag tcaggggcta cctgcgggcg
      781 tcaacgatcg ggacctggac ccggacttcg ctcgccacct atccttcggg cggtgagtga
      841 agaacgacag attccggcgg tgctttcgat cgccggatcg gactccgggg gcggagccgg
      901 tatccaggcc gacctgaagg cctttgccgc ctgcggagtc catggcatga ccgcgatcac
      961 cgcgatcacc gcccagaaca cggtcggcgt cgacttgatc gaggcgatca gccccgaaat
     1021 gatcatcgcc cagatcaagg ccgtggccgg cgacatcggg gtcgatgccg tgaagatcgg
     1081 catgctgggg gaccggccga ccatcgaagc ggtggcctcc ggtctcgacc tgcttggcga
     1141 cgttccggtc gtgctcgatc cggtgatggt ctccgaaagc ggagcgaacc tgctggcggt
     1201 cgaggcccgg gaagcactgg tcacccggat cctgccgcgg gccaccgtga cgactcccaa
     1261 catcccggaa gcacgcgcca tcacgggact ggccgaagcc ggccagcccg aacttgccga
     1321 ggtgatcgtg ggtctcggag tcaaggcggc ggtggtcacc ggcggtcaca gtgacgagta
     1381 cgtcgatttc ttcttcgacg gggaacgcgg ggtggagatc accggcgagg tttatcccgg
     1441 ccgctccgcc catggctccg gttgcaccca ttcctcggcc ctcgccgccg gcctcgcgct
     1501 ggggctcggt cccctcgaag ccgctcgcaa ggcccgcgaa ctcgcctcaa gggcggtggc
     1561 cggaagcctg gacgggatcg gttcgggtcc cggcccggtc aacgcactcg gcctgtgaaa
     1621 caaccagcct gaggagcctg ggttgaagtt atccgcacat gtgacgaata acttcaaggc
     1681 aggtggaagg cggctctagg atgggaacat gaccgggaac gacctgcctg cgaatctgcc
     1741 ccgtcctcga tcggctgcgg tcttcgatgg tcccgaccgg gcggccgccc gggcctacat
     1801 gaagggcatc ggactgagtg acgaagacct cagcaagccg accatcggaa tcgccaacac
     1861 atggaccgag gcgatgccct gcaactacgg cctgagggac ctcgcggcct atgtcaaaga
     1921 aggtgtgaag gcggccgggg gcaccccgat ggagttcaac acggtcgccg tctccgacgg
     1981 catcacgatg ggcacccagg gaatgaagag ttccctggtc agtcgcgaga tggtcgccga
     2041 ctcgatcgag ctgatggccc gcggctacca gttcgacgcg atcatcgcgc tcgccgcctg
     2101 cgacaagacg atccccggcg ccgtgatggg tgtggcccgg atcgacatcc cctcgatcgt
     2161 cctctacggc ggctcggtca agcccggcag gtacaagggc aaggacgtca gcatcctcga
     2221 ggtcttcgag gcggtcggtg cccacatcgc cggcaggatc accgacgagg aactgaccga
     2281 gatcgagaac gtggccagcc ccggttacgg cgcctgcggc ggccagttca cggccaacac
     2341 gatggcctgc gccttcgagg cactggggat ctcgccggcc ttctcggcaa tggttccggc
     2401 cgaggacaaa cagaagaccg cggtcgcgga gaaggtcggg gagctgatcc tgcaggtcct
     2461 ggccgatgac cttaagccga gccggatcat cacgcgcaag tcgctcgaaa acgcaatcgc
     2521 cgcggtctgc gcttccggcg ggtcgaccaa cggggttctt catctgctgg cgatcgcccg
     2581 tgaagccggg gtggagctgg acatcgacgt cttcgaggag gtctcccgcc gcacccccct
     2641 gctggccgac ctgaagcccg gcgggcggta cgtcgcgacc gacctctacc gggccggcgg
     2701 ggtgccgctg atcctcagaa acctcaagca gggcggcctg ctgcatgaag acgagatcac
     2761 ggtgaccggg cgcagcatcg gcgaggaggt cgccaacgcc gaagaggagc cgggccagga
     2821 cgtggttttg ccagtcgatg ccccgctcaa ggcccaggga gggctggcca tcctgcgcgg
     2881 caacatctgc cccgaagggg cggtcgtcaa ggtggcgggt acagaacgcc gccatcacac
     2941 cggtcccgcc cgggtcttcg aacgcgagga ggactgcttc gccgcggtca aacgggggga
     3001 gatcgagaag ggcgacgtga tcgtgatcag aaatgaaggg ccatcgggcg gaccggggat
     3061 gcgcgagatg ctccaggtca ccgctgccct ggtcggcgag gggatgggcg agcacgtcgc
     3121 cctgctgacc gacggacgct tctccggggc aacccggggg ctgatgatcg gccatgtctc
     3181 accggaagcg gtgaagggcg ggccgatcgg ggcgatccac gacggcgacg agatcaccgt
     3241 cgacatcgac gggcggcgcc tcgaagtgga cctcacccct gaacagatcg acgagcggct
     3301 gaagggctac tcaccgccga cgcccctcta tgatcgcggg gtcatggcga agtacgctgc
     3361 gaccgtgagt tccgcctcga tgggcgcggt cacgtaggag atgaagacga gggggcctgg
     3421 gggctcccgg gaggaaagga cagtttcatg agggagtggg gcagggccat ccgggcgata
     3481 gtcggcatcg cgctggcagg gttgttgatc ggcatcgctt cgcaacccgc cgcggcggcg
     3541 gagcagaagt tcacctggct ctgcaaaccg ggaagggcga acgatccctg ccagcagagc
     3601 ctccgcacgg agacggtctc caaggacgaa accctcggag ttcgcacgcc ccggaccctg
     3661 acccgcctga ggaacaaggt cgactgcttc tatgtctacc cgacggtgag cttccagacc
     3721 ggtcccaacg ccgacctcac cgaggacccg caggttgacg cgatcgccgg acagcaggcg
     3781 agccggttca cgcccggttg ccgcatgttc gccccgatct accgccagtt cacggtgccg
     3841 gcaatcctcg gttccgagat caccgagcag gtcaatgaca cggcctactc cggggtcaag
     3901 gccgcctgga acgagtacct ggggaggtac aacaagggcc ggggcatcgt cctgatcggc
     3961 cactcccagg gcaccggtca cctcaagcg
//
LOCUS       DAOQFI010000069         3973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10117_length_3973_cov_4.191169, whole genome shotgun sequence.
ACCESSION   DAOQFI010000069 DAOQFI010000000
VERSION     DAOQFI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10117_length_3973_cov_4.191169"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLE93_02055"
     CDS             complement(<1..826)
                     /locus_tag="PLE93_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HNH85861.1"
                     /translation="MAVDPRPGKPPSADPPKAGQPSVLPVIAIFGPTAIGKTGVAIEL
                     AELLRRKGEDPVAINCDSIQVYRGLELISGAASPEDQTRLEHRLLSFVPIDQEFSAGR
                     FGEAARGEIDSALEAGRRPILVGGTGLYLRSALSDLEMRPPVDQELRRQVELEVESRG
                     PAALHAELPPGLSARVHPNDRKRVARLTELSRSGVDPHPDHEGGGELWTSRLRHPTLL
                     VGLVEDDEPLVEKIHRRVGRMAGAGAGAEAERAVANGASRTVRAAIGFDEFQNDDLE"
     gene            complement(837..2159)
                     /gene="miaB"
                     /locus_tag="PLE93_02060"
     CDS             complement(837..2159)
                     /gene="miaB"
                     /locus_tag="PLE93_02060"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNH85862.1"
                     /translation="MKKFHVTTFGCQMNEHDSERMKGMLTSLGYSESTQRDDADLILF
                     NTCSIRENADNRFISHLGEAKRLKKADPERIVGVGGCWAQSVKDEVFRRFPFVDVAFG
                     PGQIHKLAEFLNSDQLSAQGYFEFEDFAGHLPSHRARQFQGWLQISQGCNCVCSYCIV
                     PSTRGREQSRDPRELLKEAERLAADGVREITLLGQNVNSYGRDLPKESRIRFSDLLGM
                     IDAVDGIDRLRYTSPHPKDMREDVIEAHASLPSLCEHIHLPLQSGSSSILKKMRRTYT
                     RERYMDRVAMIREQVPDCAITTDIIVGFPGETEEDFAETLEVVEEVGYDGAFTFVYSP
                     RRGTLAAEMEDQIAHPVKVERMERLVEAVQRRARERAQRFVGRNMEVLVEGPSRNDPT
                     RIRGRSRHNKAVNFEGTAEAGQMVEVEIGAATSQTLTGTERLLSGIGA"
     gene            complement(2469..2744)
                     /locus_tag="PLE93_02065"
     CDS             complement(2469..2744)
                     /locus_tag="PLE93_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85863.1"
                     /translation="MEIKTVFLIFGISLACFAVIITFIGLRSKNFPSKGALIGLLLLA
                     AFLVGGTTTFAVKLSIEEQHEREEGQKGIGEETTDAAAINIIRQDQA"
     gene            complement(2809..>3973)
                     /gene="rny"
                     /locus_tag="PLE93_02070"
     CDS             complement(2809..>3973)
                     /gene="rny"
                     /locus_tag="PLE93_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNH85864.1"
                     /translation="EEVREARLAELETVAGMTRDRARQILIREVEEEATRQAGLKVKR
                     IEEEARLDAERRARGILAVAMQRLASTHSIESNTFTIHLPNEEMKGRIIGKDGRNIRA
                     LENLTGVDIIIDETPETVVISSFDGVRREVGRLTLEKLIEDGRIHPASCEAAYEEAAA
                     KVDDVMIEAAESALLDARVTGLHPELVRLLGRLRFRTSYSQNVLSHLVECSNLAALMA
                     AELGASVEVARRAALLHDIGKAVSHEVEGTHAAVGARLARRYDEVEAITHAIEAHHNE
                     IEPVTVEAVIVQAADALSGARPGARGDSLEQYVTRLGDLEEIAGRHEGVKKVFAMQAG
                     REIRIMVDPGQVDDEATALISHEIAAAIEREMEYPGQVKITVIRELRSTRIAR"
BASE COUNT          619 a         1344 c         1257 g          753 t
ORIGIN      
        1 gctcgagatc atcgttctgg aactcgtcga agccgatcgc cgcccggacc gtccgggatg
       61 ccccgttggc gaccgcccgt tcggcctcgg caccggctcc tgccccggcc atccggccaa
      121 ccctccgatg gatcttctcg accagcggct cgtcgtcttc gaccaggccg accagcaggg
      181 tcgggtgtct aagccggctc gtccacagtt cgccgcctcc ctcgtgatcc gggtgggggt
      241 cgacgccgga acggctcagt tcggtcagcc gcgccacccg cttgcggtcg ttgggatgga
      301 cccgggccga aagcccgggc gggagttcgg catggagcgc tgccggcccc cgggactcga
      361 cttcgagttc gacctgccgg cgaagttcct gatcgaccgg gggccgcatc tccaggtcgg
      421 atagggccga gcgcagatag agcccggttc ccccgaccag gatcgggcgg cgccccgcct
      481 ccagggccga gtcgatctcg ccccgggcgg cttcgccgaa ccgcccggcg ctgaactcct
      541 gatcgatcgg aacgaaagag agcagccggt gctcgagcct ggtctggtct tccggcgagg
      601 cggcgccgct gatcaactcc aggccccggt aaacctgaat cgagtcgcag ttgatcgcga
      661 ccgggtcctc cccctttcgc cgaagcagtt cggcaagttc gatcgccact ccggtcttcc
      721 cgatcgcggt cgggccgaag atcgcgatca ccggcaggac cgatggctgc ccggccttcg
      781 gcggatctgc cgaaggcggc tttcccggtc gcggatcaac cgccatccgg ctctcctcag
      841 gcgccgatgc cgctgagcag gcgctcggtg ccggtcaggg tctgtgaggt ggcggcgccg
      901 atctccactt cgaccatctg gcccgcttcg gcggtgcctt cgaagttgac cgccttgttg
      961 tgacggctgc ggccacggat ccgggtcgga tcgttgcggc tcgggccctc gaccagcact
     1021 tccatgttgc ggccgacgaa cctctgcgcc cgctccctgg cccggcgctg gaccgcctcg
     1081 accagccgtt ccatccgttc gaccttgacc ggatgggcaa tctggtcttc catctcggcg
     1141 gccagggtgc cgcgacgggg cgagtacacg aaggtgaagg cgccgtcgta cccgacctcc
     1201 tccaccactt caagggtctc cgcgaagtct tcctcggttt ccccggggaa cccgacgatg
     1261 atgtcggtgg tgatcgcgca gtccggaacc tgctcccgga tcatcgcgac gcggtccatg
     1321 taccgctcgc gcgtgtaggt gcgacgcatc ttcttcagga tcgaactgga ccccgactga
     1381 agcggcaggt ggatgtgctc acacagcgag ggcaggctcg cgtgagcctc gatcacgtct
     1441 tcgcgcatgt cctttggatg cggactggtg tacctgagcc ggtcaatccc atcgaccgcg
     1501 tcgatcatgc cgagcaggtc ggaaaaccgg atccggcttt ccttcggcag gtcgcggccg
     1561 taggagttga cgttctggcc cagcagggtg atttcccgca cgccatcagc cgccagccgt
     1621 tcggcttcct tgagcagctc ccgggggtcg cggctctgtt cccgccccct ggtcgaaggc
     1681 acgatgcagt acgagcagac gcagttgcag ccctgggaga tctgcagcca gccctgaaac
     1741 tgccgggccc ggtgcgaggg caggtgaccg gcgaaatcct cgaattcgaa gtagccctgg
     1801 gcgctcagct gatccgagtt gaggaattcg gccagcttgt ggatctggcc ggggccgaaa
     1861 gccacgtcga cgaaggggaa tcgccggaag acctcatcct tgaccgactg ggcccagcag
     1921 ccaccaactc cgacgatccg ttccggatcc gccttcttca gccgcttcgc ctcgccgagg
     1981 tgggaaatga aacggttgtc cgcgttctcg cggatcgagc aggtgttgaa caggatcaga
     2041 tccgcgtcat cgcgctgggt cgattccgag tagccaagcg atgtgagcat gcccttcatc
     2101 cgttcggaat cgtgctcgtt catctggcac ccgaaggtgg tgacgtggaa cttcttcatg
     2161 ggcagttcag attacagtcg gccgtcaagc cacgcccaac cgaccccacg actacatcaa
     2221 cccggattca aggcacggtg ccgcaggttc gcgacctctc cggtaccaaa cctgcgtcac
     2281 cccctgatct gaaggcgcgg tcagcggggg ttaatcagcc ggggaccggt gaccctccca
     2341 aagtgccttc gcgattactg ctccgaccat cgagcaggca cgcgttcacg cgacggtaga
     2401 ggcatctttg ggagggtctc cggcccccgg cgctgcgcgt cccgggaagc cgagccggtg
     2461 cggaaggctt aggcctggtc ctggcggatg atgttgatgg cggcggcgtc ggtggtttcc
     2521 tcgccgatgc ccttctggcc ctcttcacgc tcatgctgtt cttcgatcga gagcttgacc
     2581 gcgaaggtgg tggtgccacc gacgaggaac gcagccagca gcagaagccc gatcagggcg
     2641 cccttgctgg ggaagttctt cgagcgcagc ccgatgaagg tgatgatcac cgcgaagcag
     2701 gcgaggctga tgccgaagat gaggaagacg gtcttgattt ccatcggcgc gaatcctact
     2761 gatttcgacg gcgttccggc ggcatcccga tcagcggtcc ggagctccct accgggcgat
     2821 cctggtcgat cggagttccc tgatcaccgt gatcttgacc tggcccggat actccatttc
     2881 cctttcgatt gccgccgcta tttcatgcga aatcagcgcc gtcgcctcgt catcgacctg
     2941 tccgggatca accatgatcc tgatctcccg gcccgcctgc atggcgaaga cctttttcac
     3001 cccctcgtgg cgaccggcga tctcttccag gtcaccgagc cgggtcacgt attgctcaag
     3061 ggaatccccc ctggcgccgg ggcgggcacc cgagagagcg tcggccgcct gaacgatcac
     3121 ggcttcgacc gtgaccggct cgatttcgtt gtggtgggct tcgatcgcgt gagtgatcgc
     3181 ttccacctcg tcgtaccggc gggccaggcg cgcccccacc gctgcgtggg ttccctcgac
     3241 ttcatggctc actgccttgc cgatgtcgtg aagcagggcc gcccgacggg caacctcaac
     3301 cgacgccccc agttcggccg ccatcagcgc ggccaggttt gaacactcga ccaggtggct
     3361 gagcacgttc tggctgtaac tggtccggaa ccggaggcga ccgagcagtc gcacgagttc
     3421 tggatggagg ccggtgaccc gggcgtccag cagggccgat tccgccgctt cgatcatcac
     3481 gtcatcgacc ttggcggcag cctcctcgta ggcggcttca caggaagcgg gatggatgcg
     3541 gccgtcctcg atcagcttct ccagggtcag ccggccaacc tcgcgacgca ccccgtcgaa
     3601 gctcgagatc accaccgtct ccggagtttc gtcgatgatg atgtcgactc cggtcaggtt
     3661 ctccagcgcc cggatgtttc gtccgtcctt gccgatgatc cggcccttca tctcctcgtt
     3721 cggcaaatgg atcgtgaagg tgttcgactc gatcgagtgg gtggaggcga gccgctgcat
     3781 cgccaccgcg aggatgccgc gggcccggcg ctcggcatcg agacgggctt cctcttcgat
     3841 ccgtttcacc ttcagaccgg cctggcgggt ggcttcttct tcaacctccc tgatcaggat
     3901 ctgccttgcc cggtcgcggg tcatgccggc gaccgtctcg agttcggcca gccgggcttc
     3961 gcggacttct tcg
//
LOCUS       DAOQFI010000070         3972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10122_length_3972_cov_3.418177, whole genome shotgun sequence.
ACCESSION   DAOQFI010000070 DAOQFI010000000
VERSION     DAOQFI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10122_length_3972_cov_3.418177"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLE93_02075"
     CDS             complement(<1..434)
                     /locus_tag="PLE93_02075"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNH85865.1"
                     /translation="MLRVSGHSERTDVGRQRQANEDSYMARAPLFVVADGMGGAQAGE
                     VASLTAIQAFQAGLPEGSPEAAIEQTIGVANRNIYEQAHADPSLSGMGTTITVAAVDS
                     DSEQVIIGHVGDSRAYRLRDGIIQRLTKDHSLVEEMRRRGQIT"
     gene            complement(454..918)
                     /locus_tag="PLE93_02080"
     CDS             complement(454..918)
                     /locus_tag="PLE93_02080"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNH85866.1"
                     /translation="MDYEPISVALKFGFLAVLFLFLLWIATSARKDLKRNSGGNFESD
                     TTIGAMPGGSYDAWLVVVRGGGLEAGTRFDVFGGVTLGRSSDADIAFTDRYASGLHAR
                     LYPRGDRYFLEDMNSTNGTLLDGGAVTSDAEVRDGSMIEIGDTAFRFELDRS"
     gene            complement(937..1704)
                     /locus_tag="PLE93_02085"
     CDS             complement(937..1704)
                     /locus_tag="PLE93_02085"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HNH85867.1"
                     /translation="MSVFRNLEARIEGLVEGVFSRAFSSEVQPVELARKLAKEMDSHK
                     TASVSRVYVPNEYTVHLSEKDRDKLEGYERSLEQELSGYLLEHARRKGYDLLTRPEVH
                     FNTDSRLRLGEFGIQARLVKPPVREGEMPRQADEGHTMVYKVAPQPAADPTARAPERP
                     SLTRAVVDLDDRRYVLDGPRAVLGRSDDAECVLRDPNVSRRHAELRQDAAGQWEIFDL
                     GSTNGVKINGRRVTEAPLREGDQVTIGTTTFRFGIER"
     gene            complement(1732..3288)
                     /locus_tag="PLE93_02090"
     CDS             complement(1732..3288)
                     /locus_tag="PLE93_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH85868.1"
                     /translation="MNGTIVLNRFEIGDRLGAGGFGTVYRAWDRRLEREVAVKVIETA
                     AESGPRIQREAKAAARLNHTGIVTLYEFAHHEYGREGGRAYLVSELVDGHTVRELMDR
                     DLISDREIGEIGIDICEALDHAHSREVIHRDIKPANLIVPNGRGGAKLMDFGVARLTD
                     GDDLTNTGDILGTLAYMSPEAAEGNPVTPAGDVYSLALTLFEAWTGENPRRRPTPTLA
                     LRAIERDLPLLADLRPDLPAEMTEVVDACLDREPGFRPGLEELGHALEDALPELDRSV
                     PDGNSRGAGLTSFADGGLDLGRIAAAAAVCGMVATGMIVSGAADAASVALLTVISALL
                     TLLRPKAGFLAGGVLLAAWLSVIAAMPGAALVVLLVSVPPALLIRGSGRPLALAVVGP
                     LLGTLGMAPFLPLLAAVATDWRDRVVVAVTGLVFTAFAESITGRALLFGKIEKAGTGW
                     EDSLSSAVTGLIVPVVSSPSFLLSVAVWAAVALLVGAIVGWTRGRSGGPVHEPLTIAP
                     VGSSRVPNAN"
     gene            3562..>3972
                     /locus_tag="PLE93_02095"
     CDS             3562..>3972
                     /locus_tag="PLE93_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85869.1"
                     /translation="MSEPDSDPDLELDDPGIPPPPPRRRSGGGSGRGGRPGGRGPRGG
                     GSGGDRQQLMVRRAIALGAGLLVLILLVIGAKGCLDARKNRSLDDYANSVTQIVNETN
                     ALGKSFFGRLSDPGELSVTEFTSEIQSDRSAMDGF"
BASE COUNT          645 a         1309 c         1338 g          680 t
ORIGIN      
        1 gtgatctggc cgcggcgtcg catctcctcg accagggagt ggtccttggt caggcgctgg
       61 atgattccgt cgcggaggcg gtaggcgcgg gagtcgccga cgtggccgat gattacctgc
      121 tcggaatcgg agtcgaccgc ggccaccgtg atcgtcgtgc ccatccccga aagcgacggg
      181 tcggcgtgag cctgctcgta gatgttccgg ttcgcgactc cgatcgtctg ttcgatcgcc
      241 gcttcgggac tgccctccgg caggccggcc tggaaggcct ggatcgcggt cagcgaggca
      301 acctcgccgg cctgggcacc gcccatgccg tcggcgacca cgaaaagcgg ggcgcgggcc
      361 atgtaggaat cctcattggc ctggcgctgg cggccgacgt cggtccgctc ggaatgtccc
      421 gagactctca gcatttggat gtttccgagc tactcatgag cggtcaagtt caaacctgaa
      481 agcggtgtcg ccgatctcga tcatgctccc gtcccggacc tcggcgtcgg atgtcaccgc
      541 gccgccgtcc agcagagtgc cgttggtcga gttcatgtct tcaaggaagt atcggtcacc
      601 gcgagggtag agcctcgcgt gaaggccgga cgcgtaacga tcggtgaagg cgatgtccgc
      661 gtcggaagag cggcccaggg tcaccccgcc gaagacgtca aagcgggtcc ccgcttcgag
      721 tccgcctccg cggaccacga ccagccaggc gtcgtaggag ccgccgggca tcgctccgat
      781 cgtggtgtcg gactcgaaat tgccgcccga gttccgcttc aggtctttcc tcgcgctggt
      841 cgcgatccag agcaggaaca ggaagaggac ggccagaaat ccgaacttga gggcaactga
      901 aatcggttcg tagtccaatt gcgcggccgg cgctttctac cgttcgattc cgaagcggaa
      961 ggtcgtggtt ccgatcgtga cctggtcgcc ttcgcgaagc ggtgcttccg tcacccttcg
     1021 cccgttgatc ttgaccccgt tggtcgagcc gaggtcgaag atctcccatt gaccggccgc
     1081 atcctgccgg agctcggcgt ggcggcggga aacgttcggg tcccggagta cgcactcggc
     1141 gtcgtcggag cggccgagga cggcgcgcgg tccgtcgagg acgtagcgcc ggtcatcgag
     1201 gtcgaccacg gcgcgggtca gcgagggcct ttcgggggcg cgtgcggtcg ggtcggccgc
     1261 cggctgcggc gccaccttgt agaccatggt gtggccctcg tcggcctggc ggggcatctc
     1321 gccctcgcgg accggaggct tgaccaggcg ggcctggatt ccgaactcgc cgagtcgcag
     1381 ccggctgtcg gtgttgaaat gaacctcggg cctggtcagc aggtcgtaac ccttgcggcg
     1441 cgcatgctcc agcaggtacc cggagagttc ctgttccaga gatcgctcgt aaccctcgag
     1501 cttgtcgcgg tccttttccg agaggtgcac cgtgtactcg ttcggcacat agacccggga
     1561 gaccgacgcg gtcttgtggg agtccatctc cttggccagc ttgcgggcaa gttcaaccgg
     1621 ctggacctct gaagaaaagg cacggctgaa caccccttcg acgaggccct cgatgcgggc
     1681 ttcgaggttg cggaaaacgc tcatcgccgc gaatccgtga agccaatcgg ctcagttggc
     1741 attcgggaca cgacttgatc ctacgggagc gatggtcagt ggctcgtgga ccgggccgcc
     1801 gcttcgccct ctggtccagc ccacgatggc cccgacaagc agcgccaccg ccgcccagac
     1861 ggccaccgaa aggaggaagg aaggcgagct cacgaccggg acgatcagcc cggttaccgc
     1921 tgacgaaagt gaatcctccc agcccgtccc ggccttctcg atcttgccga agagcagggc
     1981 gcgcccggtg atcgactcgg caaaggcggt gaagacgaga ccggtcacag cgaccaccac
     2041 ccggtcccgc cagtcggtcg cgaccgcggc cagcaagggc agaaacggcg ccatgcccag
     2101 ggttccgagc aggggtccga ccacggcgag ggccagcggg cgcccggacc cgcggatcag
     2161 gagggccggt gggaccgata cgagcaggac gaccaaagcc gctcccggca tcgcggcgat
     2221 cacgctgagc caggcagcga gcagcacacc cccggccagg aagccggcct tgggccgaag
     2281 cagggtgagc agggcggaga tcacggtgag aagcgcgacc gaggcagcat cggcggcacc
     2341 actgacgatc atcccggtcg ccaccatgcc gcagaccgcg gctgcggcgg caatgcgacc
     2401 gagatcgagg ccgccgtcgg cgaaggaagt cagccctgcc ccgcgggaat tgccgtccgg
     2461 caccgaccgg tcgagttccg gcagggcatc ctccagtgcg tggccaagtt cctccagtcc
     2521 gggccggaat ccgggttcgc gatcgaggca ggcgtcgacg acctcggtca tttccgccgg
     2581 caggtcgggc cgcaggtcgg ccagcagtgg caggtccctt tcgatcgccc gcaaggcgag
     2641 ggtcggggtc ggccggcgcc gggggttctc cccggtccag gcttcaaaga gggtcagggc
     2701 gagcgagtag acgtccccgg ccggggtcac cgggttgcct tccgccgctt ccggggacat
     2761 gtaggcgagc gtcccgagga tgtccccggt attggtcagg tcatcgccgt cggtcagccg
     2821 ggcgaccccg aaatccatca gtttggcgcc gccccgtccg ttcgggacga tcaggttggc
     2881 cggcttgatg tcccggtgga tcacctcgcg ggaatgcgcg tgatcaagcg cttcgcagat
     2941 gtcgatcccg atctccccga tttcccggtc gctgatcagg tcccggtcca tcagctcccg
     3001 caccgtgtgc ccgtcgacca gttccgaaac gagataggcc cggccgcctt cccggccgta
     3061 ctcgtggtgg gcgaattcgt agagggtgac gatgccggtg tggttgagcc gggccgccgc
     3121 cttcgcctcc cgctggatcc ggggaccgga ctcggcagcg gtctcgatca ccttgaccgc
     3181 gacctcgcgc tccagcctgc ggtcccaggc gcggtagacc gtgccgaatc ctccggcgcc
     3241 aagccggtct ccgatctcga atcggttgag cacgatcgtt ccgttcatca ctcagcactt
     3301 ttcggcgcga aagcccccga ccctcccctt tcgggcacca ttgtgcggtt tcgctccgtt
     3361 tcggccggga tttcggtgaa ctccctgcaa attggcatgc gggggtcgat tttccggtaa
     3421 agggaataga ttggccatgc cgaaagtcgt tcggcaggtg gacttttgga gttttcgaac
     3481 ccttacaacc cagacgaccg cgaaaacgat cctttctccg aaggggcgtc cgacaacccc
     3541 ttcgaggacg atcggacgat cgtgtcggag cccgattccg atcccgacct ggaactcgat
     3601 gatcccggca ttccgccccc gcctccgcgg cgtcgttcgg gtggcggttc cggtcgtggc
     3661 ggacgcccgg gtggccgcgg tccccgcggt ggcggttccg gtggtgaccg gcagcagttg
     3721 atggtccgcc gggcgatcgc gctcggtgcc ggcctgctgg tcctgatcct gctcgtgatc
     3781 ggagcgaagg gctgcctcga tgcccgcaag aaccgttcgc tcgacgatta cgcaaacagc
     3841 gtgacccaga tcgtcaacga gaccaatgct ctcggcaaat ccttcttcgg ccggctcagt
     3901 gatccgggcg agctctcggt gaccgagttc accagcgaaa tccagtcgga ccgctcggcg
     3961 atggacggct tc
//
LOCUS       DAOQFI010000071         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10133_length_3969_cov_3.985437, whole genome shotgun sequence.
ACCESSION   DAOQFI010000071 DAOQFI010000000
VERSION     DAOQFI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10133_length_3969_cov_3.985437"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PLE93_02100"
     CDS             complement(<1..963)
                     /locus_tag="PLE93_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNH85870.1"
                     /translation="MKGTIRQRLTFSVVCVTAVMLALLVVGFNLSLRASLDGDVDRLL
                     HARAQATLDNIDLENGKLEVSEGSDDGAEDALVWIYDDRKPLESPQVASQLDRVAASL
                     AAEGSGERDDSDSDTRLFARPVVQNGRVVGTVVTGVSLEPYERTADRAAFASVVLALI
                     MLVLITITTRLVVGRALKPVSEMTEEAAKWSEHDLDRRFNEGEPTDELTRLAATFDTM
                     LDRMAYMVRHERNFSAELSHELRTPLSAISAEAEIALTKDRDAAEYRESLERIAERST
                     ELTKILETLLDVARSEGSGGSDEAADLLDSVDSAVAAAESTESRR"
     gene            complement(960..1625)
                     /locus_tag="PLE93_02105"
     CDS             complement(960..1625)
                     /locus_tag="PLE93_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH85871.1"
                     /translation="MNLNVLVVEDNPELRRLLNQGLDEEGFEVDLARNGREALMLADP
                     QKHDLLVIDIGLPDSDGRDVCQALKSKGIEAPVLFLTAKDSGPDRVSGFSAGGDDYLT
                     KPFVFAELVARLRVLGRRLTRGDRSTSGDLRLDPVRHVLTCGDRETELTPTEFRLTGA
                     MIARSGEVIRRREMIAAAWPPGAIVHDNTVDVYVRRLRRKLDELESESRIHTVHGVGY
                     RLE"
     gene            1785..2267
                     /locus_tag="PLE93_02110"
     CDS             1785..2267
                     /locus_tag="PLE93_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85872.1"
                     /translation="MTSKIKKILASLLALAALALGGAAIASATNGGSDDDHGAQEKAE
                     VNDHDSDANENESTSEGADDNEAEDGQEAADPGEAVSSTVAAKAEAAAVARTGGTVQE
                     VTAETNDAPEKGDDQDPGEKAAPAGTAYEVDLTKGSQELKVSLDRQFNVLQVQTGQDD
                     "
     gene            complement(2372..2992)
                     /locus_tag="PLE93_02115"
     CDS             complement(2372..2992)
                     /locus_tag="PLE93_02115"
                     /inference="COORDINATES: protein motif:HMM:NF037470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HNH85873.1"
                     /translation="MEAATTSTSKAFAAHQDEAAGLGAPRATPADAFRAALRAFVRGP
                     RLDMEKLAQDLGISKTTLYRWTGPRDKLLSEILCFFGEEAARIGREASEGATGVRRIE
                     LFTRGFVESIVNFEPHRRFVRSETELAFRLVLTRGSQVQSTVVERFAEVLREEEASGN
                     LGLRAPAGDLAYAAIRIIEGFIYNDALTEDSPDVEKAVSIVCLLVD"
     gene            3122..>3969
                     /locus_tag="PLE93_02120"
     CDS             3122..>3969
                     /locus_tag="PLE93_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85874.1"
                     /translation="MKSKLIAAGAVLVAVLLTGVLGAGAASASSAKPPFYPIDKKTIK
                     VPNDVQPWLPTYSPDGKHIVFQNQLDGSTWATSKDGKGTSCITCEFSDRPDISGGFTY
                     VFPDNKRLFVSHELGGLTGVDSGPNTDAWILECSPSIYDCQSHQYLPVDMSDDKVGVP
                     LIVQRRTWHLAPDGVHLGWMDIRLDGTVMIVGRLTRLDDRYKVVDQKAVNPYRPTSLT
                     DTDAANWAGDNQLWELKSFADGGASILALGGAGYNIDPVKINLKTGKVTRLTANPDWD
                     EDGAISP"
BASE COUNT          740 a         1305 c         1237 g          687 t
ORIGIN      
        1 tctccttgac tcggtcgatt ccgccgcggc cactgcggaa tcgaccgagt caaggagatc
       61 ggccgcttca tcggatccgc cgctgccttc cgatctcgcg acgtcgagca aggtctcgag
      121 aatcttcgtg agctcggtcg atcgctcggc gatccgttcc agtgattccc ggtattcagc
      181 cgcgtcccga tcctttgtga gcgcgatctc cgcctcggcc gatatcgcgg aaagaggagt
      241 cctgagttca tgcgagagtt cggccgaaaa gttcctctcg tgccggacca tgtaggccat
      301 ccggtccagc atcgtgtcga aagtggccgc cagcctggtc agctcgtcgg tgggctcgcc
      361 ctcgttgaac cttcggtcca gatcgtgctc gctccatttc gcggcttcct cggtcatctc
      421 cgagacgggc ttgagtgccc tgccgaccac gagcctggtc gtgatcgtga tcagcacgag
      481 catgatcaag gcgagcacca ccgaagcgaa ggcggcccga tccgcggtcc gctcgtaagg
      541 ctcgagcgag accccggtaa ccaccgtgcc cactacccgg ccgttctgca ccaccggccg
      601 cgcgaagagc cttgtgtcgc tgtccgagtc atcccgctcc ccgcttccct ccgcggcgag
      661 tgaggccgcc acccgatcca gctggctggc aacctgcgga gactcaagcg gctttcggtc
      721 gtcgtagatc cagaccagcg cgtcttcggc gccgtcgtcc gatccctcgc tgacttcgag
      781 tttcccgttc tccagatcga tgttgtcgag cgtcgcctgg gccctggcgt gaaggagccg
      841 gtccacgtcg ccatcgagac tggcacgcag cgagaggttg aagcccacca cgagcagggc
      901 cagcatcacc gcggtcacgc agaccaccga gaaagtcagt cgctgacgga tcgtcccttt
      961 cattcgagcc ggtatccaac tccgtgaacg gtgtggattc tcgactcgct ctcgagttcg
     1021 tcgagtttcc tgcgaagcct ccgcacgtag acatcgaccg tgttgtcgtg cacgatcgcc
     1081 cccggtggcc aggcggccgc gatcatctcg cggcgtcgga tcacttctcc cgatcgggcg
     1141 atcatggccc cggtcaggcg aaactcggtc ggagtcagtt cggtctcacg gtccccgcag
     1201 gtcagtacgt gccggaccgg atccagcctg agatccccgg aagtggaccg atcgccccga
     1261 gtcaaacgcc tgccgagcac ccgcagccgg gccacgagtt cggcgaacac gaacggcttg
     1321 gtcaggtagt cgtctccacc ggcgctgaag cccgagaccc ggtccggtcc ggaatccttt
     1381 gcggtcagga acagcacggg tgcctcgatc cccttcgact tgagcgcctg gcagacatcg
     1441 cggccatcgg aatccggcaa cccgatgtca atcaccagca ggtcgtgctt ttgcggatcg
     1501 gcaagcatca gggcttcgcg accgttgcgc gccagatcga cctcgaatcc ctcctcgtcg
     1561 agcccctggt tgagcagccg ccggagctcg gggttgtcct cgacaaccag gacgttcagg
     1621 ttcatgtgat cgccgcttcg gtcatacggt ccaatgatcg gtggcaaacc tgaacgactc
     1681 ctgaaaatcc gctcccggtc aggagaaatt cagggttccg cgcacatact cgcctcattg
     1741 gaaaagcgcc gatgcccctc ggccccgatg agaaggaaga acacatgaca tccaagatca
     1801 agaaaatact cgcgtcactc ctggcgcttg cggctctggc cctcggcggt gccgcgatcg
     1861 cttctgcaac caacggcggc tccgacgacg atcacggcgc gcaggagaag gctgaagtca
     1921 acgatcacga ctctgacgcc aacgagaacg agtccacctc tgaaggcgct gacgacaacg
     1981 aagccgaaga cggtcaggaa gcagccgacc ccggtgaagc cgtttcaagc acggtcgccg
     2041 cgaaggctga agccgccgcg gttgccagga ccggcggaac ggtccaggaa gtgaccgccg
     2101 agaccaatga cgcgcccgag aagggcgacg atcaggaccc gggcgagaaa gccgctccgg
     2161 ccggtaccgc ctatgaagta gacctgacca agggctctca ggagttgaag gtctcgctcg
     2221 acaggcagtt caacgtgctc caggttcaga ccgggcagga tgactgaacg tgtcaagggc
     2281 ggaggaggaa cccgggtcga ctccggcccg ggcttctctt gccgcgttta cccgtgccgg
     2341 tcgacgttgc tgaagatccc gacgcagccg ctcagtcgac cagcaggcag acgatgctga
     2401 ctgccttctc gacgtcgggg ctgtcctcgg tcagggcgtc gttgtagatg aagccctcga
     2461 ttatccggat cgccgcgtag gcgaggtcac cggccggggc gcgcaacccg aggttgccgc
     2521 tcgcctcctc ttccctgagc acttctgcga agcgctcgac cacggtcgac tgaacctggc
     2581 tgccgcgggt caggacgagg cggaaggcga gttcggtttc gctgcggacg aacctgcggt
     2641 gcggctcgaa gttgacgatc gattcgacga acccgcgggt gaagagctcg atccgcctga
     2701 ccccggtcgc cccctctgac gcttcgcggc cgatccgggc cgcctcctcg ccgaagaaac
     2761 agaggatctc cgagagcagc ttgtcccggg ggccggtcca gcggtagagg gtggtctttg
     2821 aaatgccgag atcctgagcc agcttttcca tgtccagccg ggggccgcgg acgaatgccc
     2881 gcagggcggc gcggaaggcg tcggccgggg tcgcgcgggg tgcgccgagg ccggcagctt
     2941 cgtcctggtg ggcggcgaat gccttcgagg ttgacgtggt cgctgcttcc atcgaatcga
     3001 actctacaga tcggtgttac ggaaaggtgg agttgcacga aagtgtacga atatgatatt
     3061 tgtacacaat cgaatcggaa acacgacgcg tctacttgcg ccggaaatgg agagagttgg
     3121 gatgaaaagc aagctgatcg cggcaggggc cgtgctcgtc gcagtacttc tgaccggggt
     3181 tctgggggcg ggagcggcgt cggccagctc cgccaagccg cccttctacc cgatcgacaa
     3241 gaagacgatc aaggtgccga acgacgtcca gccctggttg ccgacctaca gcccggacgg
     3301 caagcacatc gtcttccaga atcagcttga cggcagtacc tgggccacct cgaaggacgg
     3361 aaagggcacc agctgcatca cctgcgagtt ctcggaccgg cccgacatca gcggcggctt
     3421 cacttatgtc ttcccggaca acaagcgtct cttcgtgagc catgaactcg gcgggctcac
     3481 cggggttgac agcggcccca ataccgacgc ctggattctc gaatgctcgc cgagcatcta
     3541 cgactgccag agccaccagt acctgcccgt cgacatgtcg gacgacaagg ttggcgtgcc
     3601 gctgatcgtt cagcgacgga cctggcacct cgcgccggac ggcgtccacc tcggctggat
     3661 ggacatccgg ctcgacggca ccgtgatgat cgtcggtcgt ctgacccggc tggacgatcg
     3721 ctacaaggtg gttgaccaga aggcggtcaa tccgtaccgg ccgacctcgc tgaccgatac
     3781 cgacgccgcc aactgggccg gtgacaacca gctctgggag ctcaagagct tcgccgacgg
     3841 cggcgcctcg atcctggccc tgggcggtgc cggctacaac atcgacccgg tcaagatcaa
     3901 cctgaagacc ggcaaggtca cccgcctgac cgccaacccc gactgggacg aggacggcgc
     3961 catctcgcc
//
LOCUS       DAOQFI010000072         3948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10231_length_3948_cov_3.045723, whole genome shotgun sequence.
ACCESSION   DAOQFI010000072 DAOQFI010000000
VERSION     DAOQFI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10231_length_3948_cov_3.045723"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLE93_02125"
     CDS             complement(<1..692)
                     /locus_tag="PLE93_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNH85875.1"
                     /translation="MATPSRAPGASRQARETQRRLARSSRVARNHLIVTVGLGFLQAV
                     LIIAQATLLAKVIAGVFMNGDSFAEVAPWLAWLAAISVGRGLAAAGFESAGRFGAARV
                     MSELREKLARHLLMNRPGALQAEQSGELVSSAIEGVAALENYFARYLPQMVLAGIVPV
                     AVLAWVLRYNWEAAAILAVTAPLIIVFMVLIGLLTEQRTRKRWERMSRLSSHFLDLVG
                     GLDGLNDGLHEW"
     gene            complement(713..1816)
                     /gene="cydB"
                     /locus_tag="PLE93_02130"
     CDS             complement(713..1816)
                     /gene="cydB"
                     /locus_tag="PLE93_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNH85876.1"
                     /translation="MEDTTTLQFVWFVLISVLWIGYLVLEGFDFGIGMLMKLIGGSRE
                     EKRAILHTIGPVWDGNEVWLLVAGGATFAAFPEWYATLFSGFYIALLAILVALIVRNV
                     GFEMWGKRDTDAWRTGWEWCIAIGSLLPALLWGVAWANIIGGVPIEPVTTHGVESIEY
                     TGTFFDLLSVYTLLGGVASLAIFFAHGALFLEIRTEGDIREKARAVAAKATPVAALLA
                     AVFMAWTLIRQDSLEILSLIAAVVAVGSFGFAAINAKSQPMRAFTGTAVGIALYFIAQ
                     FIDLFPNAMVSSIDSAFDMSLNMASSTNYTLTVMTVVAGLLVPVVLLYQGWTYWVFRH
                     RLSAEGFGDVKSPMDLLDQKKEAGSGPESDQRA"
     gene            complement(1835..3280)
                     /locus_tag="PLE93_02135"
     CDS             complement(1835..3280)
                     /locus_tag="PLE93_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNH85877.1"
                     /translation="MSTIAMTSLELARWQFGIVTLYHFIFVPLTIGLAFFTAWYQTRW
                     YRTKDEKWLRATRFWGKLMLISFALGVATGIVQEFQFGMNWSAYSRFVGDIFGAPLAM
                     EGLAAFFVESTFLGLWIFGWGRLSEKVHLACMWAVSLSTMLSAFFILAANSWMQHPVG
                     YELNQATNKVELKSIWDVLTNSTALYAMTHTMLAAALTGGMVIAGISAWHLLRKNEVE
                     VFSSSMKIALPSLAVAGLLTLVVGHFNGILMSEQQPMKMAAADAVFETEDGVGLSLFA
                     TGKFEGNPEETNRNIKLPNALSFIMTGKPDSEVKGVNNLNEEYRQRFGDPASPEYRGP
                     EFKTMDYAPIVWVAYWSWRVMLGCGFLLALFGVIGWWFNRKGKLPDSRRFLKLAMPAA
                     SLPFIAAAAGWIFTEMGRQPWVVFGLLRTQDANSPTVSATEVWISLVGMTLLYGVLAV
                     FAGKIFFKTAGKGPAEIEEGEEDKPYLGMAY"
     gene            complement(3387..>3948)
                     /locus_tag="PLE93_02140"
     CDS             complement(3387..>3948)
                     /locus_tag="PLE93_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNH85878.1"
                     /translation="FIPFGAKRKHQMARYSLRHYGVRSWRLRNPRQIVEHVAVGNSVA
                     GVYRTFAANRPDPEFHELPNVCAHFVVSGRGRVVQLVPLGIRCRHTVGLNHVSFGIEH
                     TGFTDGDVLGNPRQMRASIRLTKWLRCRYGIGIRDVIGHNESLGSRFHRELVPAMKNQ
                     THGDFRHASMRRYRKALHDAGRCPRA"
BASE COUNT          769 a         1283 c         1248 g          648 t
ORIGIN      
        1 atccattcat gcagcccgtc gttcagtcca tcgagcccgc cgaccaggtc caggaaatgc
       61 gacgacagcc gggacatccg ctcccagcgc ttgcgggtgc gctgctcggt cagcagtccg
      121 atcaggacca tgaagacgat gatcagcggc gccgtaaccg cgaggatcgc cgccgcttcc
      181 cagttgtaac ggagcaccca ggccagcacg gctacgggca cgatcccggc cagcaccatc
      241 tgcggcaggt agcgggcgaa gtagttctcc agcgcggcga ccccttcgat cgccgagctg
      301 acgagttcac cgctctgttc ggcctggagg gcacccggcc ggttcatcag caggtggcgg
      361 gccagcttct cccggagctc ggacatgacc cgggccgcgc cgaaacggcc cgcactttca
      421 aatccggccg cggcgagacc ccggccgacc gagatcgcgg ccagccaggc cagccacggg
      481 gcgacctcgg cgaaggagtc cccgttcatg aagaccccgg cgatcacctt ggccagcagg
      541 gtcgcctggg cgatgatcag gacggcctgg agaaacccca ggccgaccgt cacgatcagg
      601 tgattgcgag ctacacggct ggaccgggcc agccggcgct gtgtttcacg agcctgacgg
      661 ctcgctcccg gtgccctaga gggcgtggcc aatactctcc cctcggcccc ggctacgccc
      721 tctggtccga ctccgggcca cttccggcct ccttcttctg gtcaagcaag tccatcggtg
      781 acttcacgtc accgaaccct tcggccgaaa gacggtgccg gaagacccag taggtccagc
      841 cctggtagag cagaaccacc gggaccagca accctgcgac gaccgtcatg acggtcagcg
      901 tgtagttggt tgagctggcc atgttcaggc tcatgtcgaa ggccgaatcg atacttgaaa
      961 ccatcgcgtt cgggaacagg tcgatgaact gggcgatgaa atagagggca atgccgaccg
     1021 cggttccggt gaaggcacgc atcggttgcg acttcgcgtt gatcgccgcg aaaccgaaac
     1081 tgccgaccgc gacgaccgcg gcgatcagcg acaggatctc gagcgagtcc tggcggatca
     1141 gggtccaggc catgaagacc gcggccagca gcgccgcgac cggagttgcc ttcgccgcca
     1201 ccgccctcgc cttctcccgg atgtcacctt cggtcctgat ctcaaggaac agggccccgt
     1261 gggcgaagaa gatcgccagg gaagcgaccc cgccgagcaa cgtgtatacg gagagcagat
     1321 cgaagaaagt gcccgtgtat tcgattgact ccacaccgtg ggtcgtcacc ggttcgatcg
     1381 gcacgccgcc gatgatgttg gcccaggcga cgccccagag cagggccggc aacaggctgc
     1441 caatcgcgat gcaccattcc cagccggtgc gccaggcgtc cgtgtcccgc ttgccccaca
     1501 tctcgaaacc gacgttgcgg acgatcaggg cgaccagaat cgcgagcaag gcgatgtaga
     1561 agccggagaa aagggttgcg taccactccg ggaaggcggc gaaggtggcc ccgccggcga
     1621 ccagcagcca gacctcgttg ccgtcccaga ccgggccgat cgtatgcagg atcgcccgct
     1681 tctcttcccg gctgccgccg atcagcttca tcagcatgcc gatcccgaag tcgaaaccct
     1741 cgaggaccag gtacccgatc cagaggaccg agatcaggac gaaccagacg aactgaagtg
     1801 ttgttgtgtc ttccatggtt ttcctccggc tcccctagta ggccattccc aggtagggct
     1861 tgtcttcctc gccttcctca atctcggccg ggcctttgcc ggccgtcttg aagaagatct
     1921 ttccggcgaa caccgccagc acgccgtaga gcagggtcat cccgaccagc gagatccaga
     1981 cctcggtagc gctcaccgtc ggtgaatttg cgtcctgggt tctgagcagt ccgaacacca
     2041 cccagggttg ccggcccatc tcggtgaaga tccagccggc ggccgcggcg atgaacggca
     2101 gcgacgcggc cggcatcgcc agcttcagga aacgacgcga gtcgggcagc ttgcccttgc
     2161 ggttgaacca ccagccgatc acgccgaaca gggccagcag gaagccgcag cccagcatca
     2221 cgcgccagga ccagtaggcc acccagacga tcggcgcgta gtccatcgtc ttgaactcgg
     2281 gaccgcggta ctcggggctg gccggatcac cgaaccgctg cctgtactcc tcgttcaggt
     2341 tgttcacgcc cttgacctcg gagtcgggct ttccggtcat gatgaacgag agggcgttgg
     2401 gcaacttgat gttgcggttc gtctcttccg ggttgccctc gaacttgccg gtggcaaaga
     2461 gcgacaggcc gaccccgtcc tcggtctcga agacggcatc ggcggcggcc atcttcatcg
     2521 gctgctgctc gctcatcagg atgccgttga agtgcccgac caccagggtc agcaggccgg
     2581 caaccgcgag cgaaggcagg gcgatcttca tcgagctcga gaagacctcg acctcgttct
     2641 tgcggagcag gtgccaggcg gaaatgccgg cgatcaccat gccaccggtc agggccgcgg
     2701 ccagcatggt gtgggtcatc gcgtagagcg cggtgctgtt ggtcagcacg tcccagatcg
     2761 atttcaactc gaccttgttg gtggcctgat tcagctcgta acccaccggg tgctgcatcc
     2821 aggagtttgc ggccaggatg aagaaagccg agagcatggt cgaaagcgag accgcccaca
     2881 tgcaggcgag atggaccttc tcggagagcc tcccccagcc gaagatccag agtccgagga
     2941 aagtcgactc gacgaagaag gcggcgaggc cctccatcgc cagtggcgcc ccgaagatgt
     3001 cgcccacgaa gcgcgagtag gcagaccagt tcatgccgaa ctggaactcc tgcacgatgc
     3061 ctgtcgcgac gccaagcgcg aacgagatca acatcagctt gccccagaaa cgggtcgccc
     3121 tcagccactt ctcatccttc gtgcggtacc agcgggtctg gtaccaggcc gtgaaaaagg
     3181 ccagcccgat cgtgagcggc acaaaaatga agtgatagag cgtaactatt ccgaattgcc
     3241 agcgggccaa ctcgagactg gtcattgcaa tggtgctcat tcgtccacct tcatggatcc
     3301 cagattagct aggcaggagg tcgaaattcc tgcggaaaat caccgcagtt actgcgggga
     3361 agtacggagg tttggcttag ccccggctaa gcccgggggc atcgaccggc atcgtggagc
     3421 gccttgcggt agcggcgcat cgacgcgtgc cggaagtcgc cgtgggtctg gttcttcatc
     3481 gccggcacca gttcacggtg gaacctcgaa ccgagactct cgttgtgccc gatcacgtcc
     3541 cggatgccga tgccgtatcg gcagcggagc catttggtca gccggatcga ggctctcatc
     3601 tgtcgcgggt tgccaagcac gtcaccgtcg gtgaagccgg tgtgctcgat cccgaaggag
     3661 acgtggttca ggccgaccgt gtggcggcag cggatgccga gcgggaccag ctggaccacg
     3721 cggccccggc ccgagaccac gaagtgggcg cagacgttgg gcagctcgtg gaattcgggg
     3781 tcgggccggt tcgcggcaaa agtccggtac accccggcga ccgaattgcc cacagcaacg
     3841 tgttcgacga tctggcgcgg attccggagg cgccaggatc gcaccccgta gtgacgcagc
     3901 gagtagcggg ccatctggtg cttgcgcttc gcgccgaagg gaatgaag
//
LOCUS       DAOQFI010000073         3940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10265_length_3940_cov_3.506049, whole genome shotgun sequence.
ACCESSION   DAOQFI010000073 DAOQFI010000000
VERSION     DAOQFI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10265_length_3940_cov_3.506049"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="PLE93_02145"
     CDS             <1..1647
                     /locus_tag="PLE93_02145"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNH85879.1"
                     /translation="YLPKRPRKAASYQVDDNNFEMSADWPGYAGRKLDALNGGTAVLA
                     AGTLGRQEPPGSVTAYSEVEAQGEIVANEIQRTMAKSTPLTDDTIAASEQFMETPADN
                     DDLLLGISIFTYPGACIDGIERCTIPRSKAPPYMNEVSGEDVIGTYVEAARIGDVAFA
                     TNPGEAFPEVNFAIRDGVTGPRQVNVIAQAGDMLGYYYQRADYTDQQFGSSDFEDYNV
                     GPELGQANANSALAGLNEIGFTATPETVHAAFESSVASKPGIQWYPDEYESTDPTFNI
                     LGSAARSQDGTAPVPTSISWDFGDTTTGTTSVGERFDHTFPGPGSYEVTATVSSNGLT
                     RSWTDTITVDPPLTADASVAKRTGQGASLLGGALGGSGKIVAAHWTCQDGKKVSGLKV
                     LCKSPKAGKASVTVVDGAGNTAKATVPVAKAPPASLTIRKISVKPTKIKYGKKGTMTV
                     AVKNPSTTNATGFKVCVKVPARAKKQIKAMPACKSPGNLRAGKSASVSIRIQVTRQAS
                     PGMKVKLPVETSANGAKKKTASISILGKPGGAVSSSYAAG"
     gene            complement(1731..2294)
                     /locus_tag="PLE93_02150"
     CDS             complement(1731..2294)
                     /locus_tag="PLE93_02150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNH85880.1"
                     /translation="MTKLLIVIASVRPGRVGESVANWFVPIAEADERFEVEVADLKEL
                     DLPFMDEPNMPGRREYTKEHTKKWSAIVDRADAFVFVTPEYNATPAPALKNAIDYLYH
                     EWSYKPCSFVSYGGVWAGARAVQALKPTLTQLSVMPVPEMVPIPGVSGLLDDQGNLQG
                     TEIHEKSAGGMLNSLEKWSGALKTLRQ"
     gene            2346..3866
                     /locus_tag="PLE93_02155"
     CDS             2346..3866
                     /locus_tag="PLE93_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH85881.1"
                     /translation="MDQRSRPKSDERIDPDDLETALRVIGQLDRLPAEHPDSVTIQQA
                     AAGLYKSVKKRRKLEKRRQVLEHDAEITARTATAAPGRIDDETEGLPLVSSAKGAIAG
                     TLIEARACYICKQPFHQVDAFYHQLCPDCAAFNHARRDARTDLTGKRALLTGGRAKIG
                     MYIALRLLRDGADTTITTRFPNDAVRRFRAMKDSEEWFDRLHIVGIDLRDPGQVVDLA
                     DSIAAGGPLDILINNACQTVRRTPDAYAPLIRAEAEELEPGPVPDIETFARHGEHHLQ
                     PAHLEDGNSRWVGNPSGEPEIRPTPNAASHWAPTPHAIAAMALTAGSADFDLVDSGRA
                     IDAGGLVPDVVDTNSWIQRVNEVDPIELLEVQLCNMTAPFILISRLRQAMADSPARRT
                     YVVNVSAMEGQFSRGYKGPGHPHTNMAKAALNMLTRTSAQEMLEADGILMTAVDTGWI
                     TDERPHPMKKRLADDGFHAPLDLVDGAARVYDPIIRGEEGEDIHGVFLKDYKPSPW"
BASE COUNT          766 a         1372 c         1227 g          575 t
ORIGIN      
        1 tatctgccca agcggccgcg caaagccgcg tcctaccagg tggacgacaa caacttcgag
       61 atgtcggccg actggcccgg ctacgcgggg cgcaagctcg atgccctgaa cggcggcacc
      121 gcggtgctgg cggccggcac gctgggccgt caggaacccc cgggaagcgt caccgcgtat
      181 tccgaggtcg aagcccaggg cgagatcgtc gccaacgaga tccagcggac catggccaag
      241 tccacgccgc tgaccgatga cacgatcgcg gccagcgagc agttcatgga aacccccgca
      301 gacaacgacg acctgctgct cgggatcagc atcttcacct atcccggggc ctgcatcgac
      361 gggatcgaac gctgcaccat cccgcgttcg aaggccccgc cctacatgaa tgaggtctcc
      421 ggagaagatg tgatcggcac ctatgtcgag gccgcccgga tcggtgacgt cgcgttcgcg
      481 accaaccccg gtgaagcctt ccccgaggtc aatttcgcga tccgcgacgg tgtgaccggc
      541 ccccgccagg tcaacgtgat cgcccaggcc ggcgacatgc tcggctacta ctaccagcgc
      601 gccgactaca ccgaccagca gttcggctcg agcgacttcg aggattacaa cgtcggaccg
      661 gagctgggcc aggccaacgc caacagcgcg ctcgccgggc tcaatgagat cgggttcacc
      721 gccacgccgg agaccgtcca cgcagcgttt gaaagcagcg tggccagcaa gccgggcatc
      781 cagtggtacc ccgacgagta cgagtcgacc gatccgacct tcaacatcct cggctcggcc
      841 gccaggtcac aggacggcac cgcgccggtc ccgacctcga tcagctggga cttcggagac
      901 accacgaccg gcaccaccag cgtcggcgag cgcttcgacc acaccttccc ggggccgggc
      961 agctacgaag tcacggcaac ggtgagcagc aacggcctga cccggagctg gaccgacacg
     1021 atcacggttg acccgccact cacggccgat gccagcgtcg ccaaacggac cggccaggga
     1081 gccagcctcc tcggcggcgc cctcggcggt tccggcaaga tcgtggccgc ccactggacc
     1141 tgccaggacg gcaagaaggt cagcggactc aaggtgctct gcaagagccc gaaggccggc
     1201 aaggcctcgg tgaccgtggt cgatggcgcc ggcaacacgg caaaagcaac ggtccccgtg
     1261 gccaaggccc cgccggccag cctgacgatc aggaagatca gcgtcaagcc gaccaaaatc
     1321 aagtacggca aaaaggggac catgaccgtg gccgtgaaga accccagcac cacgaacgcg
     1381 accgggttca aggtctgcgt caaggttccg gcgagggcga agaaacagat caaggcgatg
     1441 ccggcctgca agtcgccggg taacctgcgt gccggaaagt cggcctcggt ttcaatccgg
     1501 atccaggtca cccgccaggc cagcccgggc atgaaggtga aactgccggt cgagacaagc
     1561 gccaacgggg ccaagaagaa gaccgcatcg atctcgatcc tgggcaagcc gggcggcgcg
     1621 gtgtcttcga gctacgccgc cggttgagct gacaaaggtg ccgcacgttc tgccccgtga
     1681 cggggctgaa cgtgcggcac cttgagtttt tcgggcttga gtttttcggg ttactgccgc
     1741 agggtcttca gggcgcccga ccacttttcg agcgaattca gcatgccgcc ggcgctcttt
     1801 tcgtggatct cggtgccctg gagattcccc tggtcatcga gcagcccgga aaccccgggg
     1861 atcggcacca tctccggcac cggcatcacc gacagctggg tcagcgtcgg cttcagggcc
     1921 tggaccgcgc gggccccggc ccagactccg ccgtagctga caaaggagca gggcttgtat
     1981 gaccactcgt ggtagaggta gtcaatcgcg ttcttgagcg ccggggccgg ggtcgcgttg
     2041 tattccgggg tgacgaagac gaaggcgtca gcccggtcga cgatcgcgct ccacttcttc
     2101 gtgtgctcct ttgtgtattc gcgccggccc ggcatgttcg gctcgtccat gaaggggaga
     2161 tcgagctctt tcaggtcggc cacctcgacc tcgaagcgct cgtccgcttc ggcgatcggg
     2221 acaaaccagt tggcgacgga ttcgccgacc cggccgggac ggacgctggc gatcacaatg
     2281 agaagtttgg tcatgggccg agactagagg ttgcaacctc taataccctt cgtcagttcc
     2341 tgaccatgga ccagcgcagc cgaccgaaaa gcgacgaacg gatcgacccc gacgacctgg
     2401 agaccgccct ccgggtgatc ggccagctcg accggctgcc ggccgaacat ccggattcgg
     2461 tcaccatcca gcaagccgcg gccggtctct acaagtcggt caagaaacgg cgcaagctcg
     2521 agaaacgccg gcaggtactc gaacacgacg ccgaaataac tgcccggacg gccaccgccg
     2581 caccggggcg gatcgacgac gagacggaag ggctgccgct cgtctccagc gcgaagggcg
     2641 ccatcgccgg caccctgatc gaggcccgtg cctgttacat ctgcaagcag cccttccacc
     2701 aggttgacgc cttctaccat cagctctgcc ccgactgcgc cgccttcaac cacgcccgtc
     2761 gtgacgcccg caccgacctg accggcaagc gggcgctcct gaccggcggc cgggcgaaga
     2821 tcgggatgta catcgccctg cgcctgcttc gtgacggcgc cgacaccacg atcaccaccc
     2881 gtttccccaa cgacgcggtg cgccggttcc gggcgatgaa ggacagcgaa gagtggtttg
     2941 accgtctcca catcgtcggg atcgacctgc gcgacccggg gcaggtggtc gacctcgccg
     3001 actcgatcgc ggccggggga ccgctcgaca tcctgatcaa caacgcctgc cagacggtcc
     3061 gccgcacacc ggatgcctac gccccgctga tccgggccga agccgaggag ctcgaacctg
     3121 gcccggtccc ggacatcgag actttcgccc gccacggcga acatcacctt caaccggccc
     3181 acctcgagga cggcaattcc cggtgggtcg gaaatccgtc aggggaaccg gaaatccgac
     3241 ccaccccgaa tgcggcctcg cactgggctc cgacaccgca tgcgattgcg gcgatggcac
     3301 tgaccgccgg ttcggctgac ttcgacctgg ttgattccgg ccgggcaatc gacgccggtg
     3361 gacttgtccc cgatgtggtt gacacgaaca gctggatcca gcgggtcaac gaggtcgacc
     3421 cgatcgagct gctcgaggtc cagctctgca acatgaccgc acccttcatc ctgatcagcc
     3481 ggctgcgcca ggcgatggcc gactccccgg ccagacggac ctacgtggtc aacgtctcgg
     3541 cgatggaagg ccagttctcc cgtggctaca agggccccgg ccatccccac accaacatgg
     3601 ccaaggcggc gctcaacatg ctgacccgga ccagcgccca ggagatgctc gaggccgacg
     3661 gcatcctgat gaccgcggtc gacacgggct ggatcaccga cgaacgcccc cacccaatga
     3721 agaagcgcct ggccgatgac ggcttccacg ccccgctcga cctagtcgac ggcgccgccc
     3781 gggtctacga cccgatcatc cgcggcgaag aaggcgaaga catccacgga gtcttcctca
     3841 aggactacaa gccttcgccc tggtgacctc cggttgcggg cgaggtggac atcgctgccc
     3901 tgacttgaga acctgcggtc atgagccccg aaccttccgg
//
LOCUS       DAOQFI010000074         3936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10273_length_3936_cov_3.478227, whole genome shotgun sequence.
ACCESSION   DAOQFI010000074 DAOQFI010000000
VERSION     DAOQFI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10273_length_3936_cov_3.478227"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /gene="acnA"
                     /locus_tag="PLE93_02160"
     CDS             <1..886
                     /gene="acnA"
                     /locus_tag="PLE93_02160"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576738.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNH85882.1"
                     /translation="QVVGDSIRREMFEKNYGDVFKGDENWQKVSVPEGDRYTWPDSTY
                     VRKPSFFEDMPADPPGLMPIEGARVLARLGDSITTDHISPAGAIKKDSPAGAWLIEQG
                     VGPADFNSYGSRRGNHEVMVRGTFANIRLRNQLVEKSGGFTRHFPDGEETSIYEASSQ
                     YQADGIPLVVLAGKEYGSGSSRDWAAKGTRLLGVRAVIAESYERIHRSNLIGMGVVPL
                     QYPDGESTDSLGLTGEEVITIGDLKGGEAKTVEVTAEREGADPVIFTATVRLDTPNEI
                     RYINNDGILQTVLRDLNA"
     gene            complement(908..2860)
                     /locus_tag="PLE93_02165"
     CDS             complement(908..2860)
                     /locus_tag="PLE93_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNH85883.1"
                     /translation="MPVSVSLEGQPYSASWPDSASFTARLKVEAGSQGADFGFQLDPT
                     GWGLEGVGFGSVFRDGQAVLDGPGELTSNPIGSGGPSGLPGYCSRGGQVPNFDYRLKL
                     LPDTSTVVEIPVSLNASPLEGMNSDLRIKYYASSPTGISPTIDAPVQVGGKTGVLVRF
                     DTGSSGQSPLTANNREPIRLSGSTAPSLPNTEILIKATPFAASPPAPPVRSISVTTDA
                     EGNFSREVVFDRSAFWRLTAHPMVDSSHDDEPSCGPTLVTTVDTYGDLPAEAGLSASA
                     PVSWPDQKDFEYRLSLTAGDHGADFNFEFQYSDWGLSGVSGTPFSIGEASLEGPGSLT
                     TVSTSVADPLPTACWRGGFGSVFNTYNLKLDPNQETVVTVPATLLAPRLAGMKPEIGA
                     VLSAGDGEPQELTAPVTITGEEGIRIIARVAGATGSVAALAPGLPFRLVGRTVPAVKN
                     RLIRLRVEPRVWEFGKLKPAPVAHRIAVVRTDGNGRFRSQPVTLGREAQWVLTSRLLN
                     AGEFDNRPNCNGTLDSAGSAPTTGELIGRSYRSVAVRGTKLKKPLKISFATGPTMRRD
                     ENGKQIEGPLLLAYVDCNGMGGEYKVRDGRLTWFSVYSTAMYCPGSKDGWLLGLLGRG
                     ARARTNGSLLTLSRGNQRLVLRETGQ"
     gene            complement(3449..>3936)
                     /locus_tag="PLE93_02170"
     CDS             complement(3449..>3936)
                     /locus_tag="PLE93_02170"
                     /inference="COORDINATES: protein motif:HMM:NF028180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HNH85884.1"
                     /translation="LVVFTPKGLLRQTAATATLEQLTGEHFHFILDDPKATERREKVE
                     RLVLCSGRIYHDIDAAASREEAEKVAVARVELLYPFPREQLAELFKAYPNLKEVVWVQ
                     EEPKNMGAWATMWRRLPPIMPEGVNLEYIGRPERSSPSEGYSVAHIREQERIVLTALT
                     P"
BASE COUNT          707 a         1265 c         1314 g          650 t
ORIGIN      
        1 tcaggtggtc ggcgattcga tccgccgcga gatgttcgag aagaactacg gcgatgtctt
       61 caagggcgat gagaactggc agaaagtctc ggtgcccgag ggtgaccgct acacatggcc
      121 ggattcgacc tatgtccgca agccctcctt cttcgaggac atgccggccg acccgcccgg
      181 ccttatgccg atcgagggag cccgggtgct cgccaggctg ggcgactcga tcaccaccga
      241 tcacatttcg cccgccggag cgatcaagaa ggacagcccg gccggtgcct ggctgatcga
      301 gcagggagtc ggcccggccg acttcaactc atacggctca cggcgcggca accacgaggt
      361 gatggtgcgc ggcaccttcg ccaacatccg cctccgcaac cagctggtcg agaagtccgg
      421 cggcttcacc cgccacttcc cggacggcga ggagacttcg atctacgagg cttccagcca
      481 gtaccaggcc gacggcatcc cgctggtcgt cctggccggc aaggagtacg ggtcgggttc
      541 atcccgtgac tgggccgcca agggcacccg cctgctcggg gtccgcgcgg tcatcgccga
      601 aagctacgag cggatccacc gctcgaacct gatcggcatg ggtgtggtcc cgctccagta
      661 cccggacggg gaatcaaccg attcgctcgg cctgaccggc gaagaggtga tcacgatcgg
      721 tgacctgaag ggcggcgaag cgaagacggt cgaagtgacc gccgagcggg aaggagccga
      781 tccggtcatc ttcaccgcga cggttcgcct cgacaccccc aatgaaatcc gctacatcaa
      841 caacgacggg atcctgcaga ccgtcctgag ggacctgaac gcctgatccg gaaagatctg
      901 cgccggccta ttggccggtt tcacgcagga ccagccgctg atttccccgg ctcagagtca
      961 ggagggaacc gtttgtccgt gcccgggcgc ccctgcccag cagaccgagc agccagccgt
     1021 ccttcgagcc ggggcagtac atcgcggtcg aatagacgct gaaccaggtc agcctgccgt
     1081 ccctgacctt gtactcgccc cccattccgt tgcagtcgac gtaggcaagc aacagcggcc
     1141 cttctatctg tttgccgttc tcgtcgcggc gcatggtcgg accggtcgcg aagctgatct
     1201 tgaggggttt cttcagcttg gttcccctga ctgccaccga tctgtaggag cggccgatca
     1261 gttccccggt cgtcggtgcg cttcccgccg agtccagggt tccgttgcag ttcggcctgt
     1321 tgtcgaactc cccggcgttg aggagccggc tggtcaggac ccactgggct tcccggccga
     1381 gcgtgaccgg ctggctccgg aaccggccgt tgccgtcggt gcggacgacg gcgatccgat
     1441 gcgcgacggg tgcgggtttc agcttcccga attcccagac ccggggctcg acccgcagcc
     1501 ggatcagccg gttcttcacg gccggcacgg ttcggccgac cagtcgaaat ggaagcccgg
     1561 gggcgagcgc ggcgacggac ccggtcgctc cggcaaccct ggcgattatg cggatccctt
     1621 cctcgccggt gatcgtgacc ggcgcggtca gttcctgcgg ctcaccgtcc ccggccgaaa
     1681 gcacggcccc gatctccggc ttcatgccgg ccaggcgggg agccagcagg gtggccggaa
     1741 cggtgaccac ggtctcctgg ttcgggtcga gtttgaggtt gtacgtgttg aacacgctgc
     1801 cgaagccacc ccgccagcag gcggtcggca aaggatcggc gacggatgtc gaaacggtgg
     1861 tcagggaacc ggggccctcg agcgaggcct ccccgatcga aaacggagtt ccgcttaccc
     1921 cgctcaggcc ccagtccgaa tattgaaact cgaagttgaa atcagccccg tgatcaccgg
     1981 cagtgagaga tagccggtac tcgaagtcct tctggtcggg ccacgaaacc ggggcagaag
     2041 cgctcaggcc ggcctcggca ggcaggtcac cgtaggtgtc aaccgtggtc acgagggtcg
     2101 ggccgcaact cggttcatcg tcgtgggagg agtcaaccat cgggtgggcg gtgaggcgcc
     2161 agaacgcgct gcggtcgaag acgacctccc tgctgaagtt gccctccgcg tcggtggtga
     2221 cgctgatcga gcggaccggc ggagctggcg gcgatgccgc gaagggcgtc gccttgatca
     2281 ggatttcggt gttgggcagg gacggggcgg tggatccgct gagccggatc ggctcccggt
     2341 tgttggccgt gagcggactc tggccgctgc tcccggtgtc aaacctgacc agcactccgg
     2401 tcttgccccc gacctgaacc ggcgcgtcga tcgtcgggga aatgccggtc ggcgagctgg
     2461 cgtagtactt gatccgcagg tccgagttca tgccttccag cggcgaagca ttgaggctga
     2521 ccgggatctc gaccaccgtc gaggtatcgg gaagaagttt cagtcggtag tcgaagttgg
     2581 gcacctggcc gccccggctg cagtatccgg gcaacccgga gggcccaccg gagccgatcg
     2641 gattcgaggt cagctcaccc ggtccgtcga gaaccgcctg cccgtcccgg aacacgcttc
     2701 cgaagccgac gccttcaagg ccccagccgg tcgggtcgag ctgaaacccg aagtcagctc
     2761 cctgcgaacc ggcttcaacc ttcaaccggg cagtgaagga agccgaatcc ggccaactgg
     2821 ccgagtaggg ctgaccctca agcgaaacag aaacaggcac cgttcccgcc tgggcggcaa
     2881 ccggaaggac accggccgcg agaagagcgg caaggaaaac aaaaaacccc ctgaaagtcc
     2941 tcatgacaaa aaagagcata aacgggaagc cggtatctgg cgacaaagct cagggtctgg
     3001 tcagtggtgc caaccagcgg agccggccca accgtcagtg aatgtgagtg ggccggcggg
     3061 tcaaaaagaa agggccggcg tagaccggcc cggccaccta aaaagtggcg ggcgagggtc
     3121 gcgggaggca gggggtccgc gcgaccctca ccacgcctgc gcgtcggtag ccagggaggg
     3181 atgccgacgc gaacgcttcc gggggtaaac cgccggaagc gggcttggaa aatttcgact
     3241 ggtggcaacc cctccggggc gctggtccga tcctgctcct tcccttttct ccctactgcg
     3301 actgcggacc tgcaacgcag tttatacacc tattgacgat gtgcgtcaac gaggaacgaa
     3361 tacgcagtcc ggtcttagcc ggaatgcggc ggaaacccgc cgccccggag agtcaaatcg
     3421 tcaccgaggc ggccccggcc gaggtggctc agggagtgag agcggtcagg actatgcgtt
     3481 cctgctcgcg gatgtgggcc accgagtagc cctccgacgg gctggagcgt tccggccggc
     3541 cgatgtactc gaggttcacg ccctcgggca tgatcggcgg cagtcgcctc cacatcgtcg
     3601 cccaggcgcc catgttcttc ggttcttcct ggacccagac cacttccttc aggttcgggt
     3661 aggccttgaa cagttcggcc agctgctcgc gggggaaggg gtagagcaac tcgaccctgg
     3721 ccacggccac cttttcggct tcctcgcggg aagcggccgc gtcgatgtcg tggtagatcc
     3781 ggcccgagca gagcaccagc ctttcgacct tttcccggcg ttcggtggct ttggggtcgt
     3841 cgaggatgaa gtggaagtgc tcgccggtca gctgctcgag cgtggcggtg gccgcggtct
     3901 ggcggagcag gcccttcggg gtgaagacga ccagtg
//
LOCUS       DAOQFI010000075         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10371_length_3917_cov_2.990678, whole genome shotgun sequence.
ACCESSION   DAOQFI010000075 DAOQFI010000000
VERSION     DAOQFI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10371_length_3917_cov_2.990678"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PLE93_02175"
     CDS             <1..1084
                     /locus_tag="PLE93_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85885.1"
                     /translation="VEGEVESRSSLFSGVSATGLRTIGAALLIVPCGVLVSSALAYHE
                     SWLAPRAQLAELSQIGQEFKGQGPALMTEYQAYGVRHFLRLLDAEGASELRRRVIPRL
                     DGTQAEKGSWSDTDELVMDPGQQGVLTYRTLVLRRNPKQSRPPSPYELVWQGRYYEVW
                     QRDPDYEASRLIVHHPLGGGFQPAAVPNCAVVQNVAAQAGQTGKVVAALREPNAVANL
                     TEYPPDWVPDPPNKTLTPLSDGTASGEVEIPRSGEYHVWVGGSARGKVSVEIGGQNAG
                     SARGMLNNNAQFMGLDDLDFEAGTDPVAMTYKKGSDLRPATGGYPFGIGPVVLAPVAE
                     PKVVTVSPANAQKLCGKSLDWVEAVR"
     gene            1382..>3917
                     /locus_tag="PLE93_02180"
     CDS             1382..>3917
                     /locus_tag="PLE93_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85886.1"
                     /translation="MSFDVPANVAGTRNTSAFVNNPSDPYLGNNSASAPITIIASADI
                     AVSVDQPSAMRIGDTSSITYSVRNIGTAPTTGSPSVKLVVFMPASLEPVSTNSHDGWE
                     CSAVPRSGNQNARFDCVFPSEMGPGDVTSLNARVRVLQTNDTQTDTLVGAESAGDVNQ
                     LNNTASAFSSITGIDLKATVAPVSGQEDTEAGVTSRRVVTVTNVGTAVTTGPVRVEVP
                     LPAGVSWTPAPPVGTGWECVQLLAQTISCVQSNSLAPGASAPALNIDIQPSRTNAPSV
                     TIPYTVSTTNDENAENNTATRTDTVLYKPETTILSAPSGTTTSRTARIEFEADDHAAT
                     FECKLDTGSFSTCASPVELNEVPLGGHTLSIRAINERGMVEETVAEANWTVITEAPVG
                     ESTGLQATLTGGTLSLAALGEVELPASQLKLRGSRFQSGAWSIPQSGFGFEPIVQVID
                     APGIGEVSVKISISSTGPGVGSLPSGGGAASLNLPVQAKLEASVGGAPLIGPEADCAL
                     RPITFDLSGTYDEAGKTVSLGSPGITFPQVSAGCGSLGATVNSLLELPRSDIAMNLVF
                     ALEQIGNNAVVKPAAPAITSGPSAPVSTKAASFAFSGDLGSTFECQLDDGDWAACSSP
                     KAYSSLAEGAHTFRVRQTSDSGQTSDPATRSWTADTTAPDAPTITAGPSGTVGFSSTS
                     ISFTGESGASFACSLDGGPFEVCVSPKAYQSLADGDRTFRVRQIDAAGNTSATSASRS
                     WTINSDSTQSRLEATLTGGSLSLAALGEVELPADQLKLIGQRAANGTWSVPQSGVIFE
                     PIEQTIDAPGIGSVTVKISISATADGAGSLPTGGGAATLNLPVQAKL"
BASE COUNT          743 a         1090 c         1254 g          830 t
ORIGIN      
        1 agtagagggt gaggttgaga gtaggtcgag ccttttctcc ggggtttcgg cgaccggact
       61 tagaacgatc ggggcggcgc tgctgatcgt gccttgcggg gtcctggttt caagtgctct
      121 ggcctaccac gaatcctggc tggcgccaag ggcacaattg gcggagcttt cccagattgg
      181 tcaggaattc aaagggcagg gtccggctct gatgaccgaa taccaggcct atggagtccg
      241 ccatttcctg cgattgctgg acgcagaagg tgcctcggag cttcgtcggc gggtgattcc
      301 ccgtctggac ggaacgcaag ccgagaaagg ctcctggtcg gacactgacg agttggttat
      361 ggaccccggt cagcagggcg tgctgacata ccggaccctg gtgctgcgtc gcaacccgaa
      421 gcagagccgg cccccttcgc cctacgagct ggtctggcag ggtaggtact acgaggtttg
      481 gcagcgtgat cccgattacg aagccagccg gctgatcgtc catcacccgc tgggcggtgg
      541 cttccagcct gctgcggtgc cgaattgtgc ggtggtccag aacgtggcgg cccaggccgg
      601 gcagactggc aaggtcgttg ccgcgctgcg tgaaccgaac gctgttgcca acctgactga
      661 gtatccgcct gactgggtgc cagacccgcc caacaagacc ctgaccccgc tttccgacgg
      721 aacggcctcc ggcgaggttg agatcccgag atccggcgag tatcacgtct gggtcggcgg
      781 ttcggcgcgc ggcaaggttt cggtcgagat cggcggacag aatgcaggct cggctcgcgg
      841 catgctcaac aacaacgccc agttcatggg cctcgacgac ctcgacttcg aggccggaac
      901 cgatccggtc gcgatgacct acaagaaggg gagcgaccta cgacccgcta ccggcgggta
      961 ccccttcggc atcggcccgg tggtcctcgc accggtggca gaaccgaagg tagtcacggt
     1021 ctccccagca aatgcacaga aactttgtgg aaaatctctg gactgggttg aggcagtccg
     1081 gtgatcggac cctcctgcat tcgcgcagat taattcggct cggcttcggg catagctgaa
     1141 tctcactacc tagaaacatc attgtgccga ttcgtgaacg ccgtttccgg gtttgcgaga
     1201 taccgcaaat gtctatgaaa attcggcttc acacagggga aagcatgcgt tactgtgcgg
     1261 cgctaagggc cagggaatgg cccgtgtcaa acgtccaata cccaggcagc agggagacaa
     1321 agatctgacc tgtaccagga cagcgccgat cgcaccgggt actacgacgc cacagatcac
     1381 ggtgagtttc gatgtaccgg ccaacgtggc tggcaccagg aatacttcgg ccttcgtaaa
     1441 caaccccagc gatccctatc tcgggaacaa ctcggcttcc gcgccgatca cgatcatcgc
     1501 ttccgccgac atcgcggtct cagttgatca accttcggcc atgcgaatcg gggacacgag
     1561 ttcaattacc tacagcgtgc ggaacattgg caccgctccg acaacaggtt caccgtcagt
     1621 gaagctcgtg gtgttcatgc cggcgagcct ggagccggtg agtaccaaca gccatgacgg
     1681 atgggaatgt tccgcggttc cacggagcgg taaccagaac gccaggttcg actgcgtctt
     1741 cccgagtgaa atgggtccag gtgacgtgac ttccctcaac gccagggtgc gcgttctcca
     1801 gaccaacgac acgcagacgg acacactggt gggcgcggaa tcagcaggtg acgtgaacca
     1861 gctgaataac accgcctcgg ccttttcctc gattaccgga attgatctga aggcgacggt
     1921 cgccccggtc agtggtcagg aagacaccga ggcaggagtg acaagccgcc gcgtcgttac
     1981 ggtcaccaac gtcggtacag cggtaacaac cggaccggtt cgcgtggagg tccccctgcc
     2041 ggcgggcgtt tcctggacac cggctccccc ggtaggaacc ggttgggagt gcgtccagct
     2101 gctcgcccag accataagtt gcgtacagag caacagcctc gcacccggtg cttcagcccc
     2161 agctctcaac atcgatatcc agccgagtcg gaccaacgca ccgtcggtca ctattccgta
     2221 caccgtgagc acaactaacg acgaaaacgc tgaaaacaac acggccacga gaacggacac
     2281 ggttctctac aagccggaaa ctacgattct ctcagctccc tccggaacta ccacttcaag
     2341 gacggcccgg atcgagttcg aggccgacga tcacgcggct acattcgaat gcaagctgga
     2401 taccggttcg ttcagcacat gcgccagtcc cgtagaactc aatgaagtcc ccttgggtgg
     2461 tcacactctc tcaatcagag cgattaacga gcggggaatg gttgaggaaa cggtggccga
     2521 ggccaactgg accgtcatta cggaagctcc ggtcggggag agcaccggtc tccaggccac
     2581 gttgaccggg ggtacgttgt cgttggctgc gttgggtgag gtggagttgc ctgccagcca
     2641 gttgaagctc aggggcagca ggttccagtc tggtgcctgg tcgattccgc agtcgggttt
     2701 tggttttgag ccgattgttc aggtgattga tgcgcctggt attggtgagg tgtcggtgaa
     2761 gatttcgatt tcttcgactg ggcctggtgt gggttcgttg ccgtctggtg gtggtgcggc
     2821 ctcgttgaat ttgccggttc aggcgaagct tgaggctagt gttggcgggg cgccgttgat
     2881 tggtcctgag gctgattgtg ctttgaggcc gatcacgttt gatttgtctg gtacttatga
     2941 cgaggcgggc aagactgtga gtcttggttc tccgggtatc acgttcccgc aggtgagtgc
     3001 tggttgtggt tcgcttgggg cgacggtgaa tagtttgttg gagttgccgc gtagcgacat
     3061 tgcgatgaat ctggtgttcg ctttggagca gatcggtaat aacgctgtgg tgaagccggc
     3121 tgctccggcg atcacttcgg gtccgagtgc tccggtgagt acgaaggcgg cttcgtttgc
     3181 tttcagtggt gatctcggtt cgacgttcga gtgtcagctt gatgatggtg attgggcggc
     3241 ttgttcttcc ccgaaggcct acagttcgct ggctgagggt gctcatacct tcagggtcag
     3301 gcagaccagc gattcgggtc agacttcgga tccggctacc aggagttgga cggctgatac
     3361 gaccgctccg gacgcaccga ccattacggc tggtccttcg ggcacggtcg ggttctcgtc
     3421 gaccagcatc tcgtttacgg gtgagtctgg tgcgtcgttt gcctgcagcc ttgatggtgg
     3481 accgttcgag gtttgtgttt caccgaaggc ctaccagtct ttggctgatg gtgacaggac
     3541 tttccgggtc aggcagatcg atgcggccgg taacacttcg gcgacgtcgg cttccaggtc
     3601 ctggacgatc aattccgata gcacccagtc gaggcttgaa gcgacgttga ctggtggcag
     3661 cctgtctttg gcggcgcttg gtgaggttga gttgccggct gatcagttga agctgattgg
     3721 tcagcgggcc gctaatggca cctggtcggt tccgcagtca ggggttattt tcgagccgat
     3781 cgagcagacg atcgatgctc cgggtatcgg atcggtcacg gtcaagatct cgatttcggc
     3841 tactgccgat ggtgccggtt cgttgccgac aggtggcggg gcggcaacgt tgaacctgcc
     3901 ggttcaggcg aagctcg
//
LOCUS       DAOQFI010000076         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10394_length_3913_cov_2.996890, whole genome shotgun sequence.
ACCESSION   DAOQFI010000076 DAOQFI010000000
VERSION     DAOQFI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10394_length_3913_cov_2.996890"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLE93_02185"
     CDS             <1..348
                     /locus_tag="PLE93_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85887.1"
                     /translation="ADDGVHGHELWHLFEDDEVGIVVPAQSPKIRNQATSISLGCPAS
                     EQAGPCRGSLKISRKGRTLGRGSFRIGAGHTGKPVVKLGPRVAAGPVSLEVTVRDDAG
                     NSKTVSRKLRLSR"
     gene            345..1697
                     /locus_tag="PLE93_02190"
     CDS             345..1697
                     /locus_tag="PLE93_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85888.1"
                     /translation="MRPLARIAAVAVALISAGLIAGTAQAAVRAELAADVDPGPSSSR
                     PSKLTAVGDSVYFYADGGPDRQGLWRTNGRLTQLVRPVGDPDLRIYWTEKVAFQGRLY
                     LMGSETGNNYRLWRTVPGGGRIEPVPGWPKELKAGSKPTVANGLLFFRGGDGLHKNEL
                     WKYDGERASLVKDINPGPAGSFPGYITGLGNQVIFNATDGVHGGELWRSDGTASGTRM
                     IGEINPGAKGTNMAWLTRVGDSVYLTADTPGHWLEPWRTDGEQLGLVAAEHEGPEWDN
                     ITSFAGLGGKVYFTANNELWESDDAWPEAEVHPIADDPDFPTTAKNAPVAAGNRIFFA
                     SFAGDWTGTMQSWPSFYDGEKIVTLTDPEEEFDYPWGYPQFFKEFGGRVFFVGADTLH
                     GTELRVVSGRHYRLFADILEGDSSSRPRGFARSKHFMYFVADDGIHGREIWRLRRTAG
                     "
     gene            1850..3583
                     /locus_tag="PLE93_02195"
     CDS             1850..3583
                     /locus_tag="PLE93_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85889.1"
                     /translation="MYPEIHRLRFVLAAASAITLLVISGMLMLGVTNADSAMVSHRFT
                     DINPGASSANPYSFTRVGGDLYFTASDGQHGSEIWRLSDGRTEMVADVNPGKHGSEPG
                     YLTAFQGQLYFRAYSEATGVEMWRTDGETTELLYDIAPGDESSEPYSPTVLGDRLIFT
                     ADNGTSGSEPWMFDGNTLALVQDLRAGAVGSYPAGYTALGDYLYFRADNGVLGTELYR
                     TDGNSATLVANISPGASSSGPEDFTRFGDYLYFSATNGVGDEMWRTNGSSTAIVKDLY
                     PGANGSSPTHFTVMGDHLYFRATDGVTGLELWRTDGAVPAANVEPVADILPGAGSSYP
                     GQFAVMGGHLYFQAENGPVGEELWRTAGTGAEMVGDIAPGPEDSNPAGLITFGSSVYF
                     IANDGTHGSEVWSTNGGQPHLVRDILEGSDGSSPNQFTVYGNSIYFSADDGVSGTEIW
                     RVTGDSTVKVKLPSAAVRVDRFGRASLKVRCAASEISGPCRGTVVVKTRSKVKTGGKR
                     RQVVIGKALFTAEPGKAGRVSLKLSPKVRRLVRKSGQARRVSVRVKARDGVGNTSTLT
                     RNARLTGPGAR"
     gene            3656..>3913
                     /locus_tag="PLE93_02200"
     CDS             3656..>3913
                     /locus_tag="PLE93_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNH85890.1"
                     /translation="MPSGSEESRLVRAAASGDGSAFASLYETYERPIFNYVLRLLGDR
                     HEAEDATQDAFIRVMAKLPELDLDRFEFAPYLYTAARNAGYD"
BASE COUNT          692 a         1324 c         1292 g          605 t
ORIGIN      
        1 gccgacgacg gagtacacgg gcatgagctc tggcatcttt tcgaggatga cgaggtcggc
       61 atcgtggtcc cggcgcagtc gccgaagatc cgcaaccaag ccacgagcat cagcctcggt
      121 tgcccggctt cggagcaggc cgggccctgc cggggcagcc tgaagatctc gcgcaagggc
      181 aggaccctcg gcaggggaag cttccggatc ggggcgggtc acaccgggaa gccggtcgtg
      241 aaactcgggc cccgggtcgc ggccggtccg gtttcgcttg aggtcaccgt gcgcgacgac
      301 gccgggaatt cgaaaacggt cagccgcaag ctgaggctct cccgatgagg ccgctggcac
      361 ggattgcggc cgtggccgtg gccctgatct cggccggtct gatcgcggga acggcccagg
      421 ccgcggtccg ggccgaactc gcagccgacg tcgacccggg cccgagcagc tcccggccga
      481 gcaaactgac cgcagtcggt gattccgttt atttctacgc cgacggcggc ccggaccggc
      541 agggcctctg gcgcaccaat ggcaggctga cccagctggt ccggccggtc ggcgatcccg
      601 acctgcggat ctactggacc gagaaggtcg ccttccaggg ccggctttac ctgatgggat
      661 cggaaaccgg gaataactac cggctctggc ggaccgtccc gggaggagga aggatcgaac
      721 cggttccggg ctggccgaaa gagttgaagg cagggtctaa gccgaccgtc gccaacggcc
      781 tgctcttctt caggggcggt gacgggctgc acaagaacga actctggaag tacgacggcg
      841 aaagggccag cctggtcaag gacatcaatc cgggccccgc cggttccttt cccggataca
      901 tcacaggcct cggcaaccag gtgatcttca acgccaccga cggggtccac ggcggcgagc
      961 tctggcgttc cgacggaacc gcttcaggga cccggatgat cggggaaatc aatccgggcg
     1021 ccaagggcac caacatggct tggctgacca gggtggggga ttcggtctat ctcaccgcgg
     1081 acacgcccgg ccactggctc gagccctggc gcaccgacgg agagcagctc ggactcgtcg
     1141 cagccgaaca cgaaggcccg gaatgggaca acatcacctc gtttgcgggc ctgggcggca
     1201 aggtctactt cacggcgaac aatgaactct gggaaagcga cgacgcctgg ccggaggctg
     1261 aggttcaccc gatcgcggac gacccggact tcccgaccac ggccaagaac gccccggtcg
     1321 cggccgggaa ccggatcttc ttcgcctctt tcgccgggga ctggaccgga accatgcaga
     1381 gctggcccag cttctacgac ggcgagaaga tcgtcaccct gaccgacccc gaagaagaat
     1441 tcgactaccc ctggggctac ccgcagttct tcaaggagtt cgggggaagg gtcttcttcg
     1501 ttggcgcaga caccctccac ggcaccgagc tgcgggtcgt cagcggccgt cactaccggc
     1561 tgttcgcgga catcctcgaa ggggacagca gcagccggcc acgcgggttc gcccggtcga
     1621 agcacttcat gtacttcgtt gccgatgacg gcatccacgg ccgcgagatc tggcgcctgc
     1681 gccggaccgc gggctaaagt ccccggcgcc cggcccggcg ggtcgctcgc ctgccccgga
     1741 aacggggctt cgctgaagtt ttcccgggcc cgcgtaagaa accggccggt tcctcgttac
     1801 acggatgaaa gggcttttcg aagccgcaat caccaagaga ggaatcacga tgtacccaga
     1861 aattcaccgg cttcgattcg tactggcggc cgcttcggcg atcaccctgc tggtcatctc
     1921 tggcatgctg atgctgggtg tcaccaacgc cgactcggcg atggtctcgc accgcttcac
     1981 cgacatcaat cccggggcca gcagcgccaa cccgtacagc ttcacccggg tcggcggcga
     2041 cctctacttc acggccagcg acggccagca cggcagcgag atctggcgtc tgagcgatgg
     2101 caggaccgag atggtcgccg atgtcaaccc cggcaagcac ggctccgaac cgggctacct
     2161 gaccgcgttc cagggccagc tctacttccg ggcctactcc gaagcgaccg gggtcgagat
     2221 gtggcgaacc gacggcgaga cgaccgaact gctctacgac atcgctcccg gtgacgagtc
     2281 atccgagccg tacagcccga ccgtgctcgg cgaccggctg atcttcaccg ccgacaacgg
     2341 cacttccggc agcgagccct ggatgtttga cggcaacacc ctggccctcg tccaggacct
     2401 ccgtgcgggt gcggtcggtt cgtaccccgc cggctacacg gcgctgggtg actacctcta
     2461 cttccgggcc gacaacggcg tgctcgggac ggagctctac cggaccgacg gcaactcggc
     2521 aaccctggtc gcgaacatct cgccgggagc ttccagttcc ggtccggagg acttcacccg
     2581 cttcggcgac tacctctact tctcggcgac caacggggtc ggcgacgaga tgtggcggac
     2641 caacgggtcc tccacggcga tcgtcaagga cctctacccc ggagccaacg gatcctcgcc
     2701 gacgcacttc acggtgatgg gcgaccatct gtacttccgg gcgaccgacg gggtcaccgg
     2761 cctggaactg tggcgcaccg acggtgccgt gcccgccgcg aacgtcgagc ccgtggcgga
     2821 catcctgccc ggtgccggca gctcctaccc cggccagttc gcggtcatgg gtggccacct
     2881 ctactttcag gccgagaacg gtccggtcgg agaggaactc tggcgcacgg cgggaaccgg
     2941 cgccgagatg gtcggtgaca tcgccccggg ccccgaggat tcgaacccgg ccggtctcat
     3001 caccttcggt agcagcgttt atttcatcgc caacgacgga acccacggat ccgaggtctg
     3061 gtccaccaac ggtggccagc cgcacctggt ccgggacatc ctcgaaggca gcgacggcag
     3121 cagtcccaac cagttcaccg tctacgggaa ctcgatctac ttctcggccg acgacggagt
     3181 ttccggcacc gagatctggc gggtcaccgg tgacagcacg gtcaaggtca agctgccctc
     3241 cgcggccgtc cgggtggacc ggttcggccg ggcgagcctg aaggtccgtt gcgcggcctc
     3301 cgagatctcg ggaccctgcc gcggaaccgt ggtggtgaag accaggagca aggtcaagac
     3361 gggtggcaag cgtcgccagg tcgtgatcgg gaaggccctg ttcaccgcgg agccgggcaa
     3421 ggccggccgg gtcagcctga agctttcgcc gaaggtccgc cggctggtcc ggaagtccgg
     3481 ccaggctcgc agggtgagcg tccgggtcaa ggcccgggac ggggtcggca acacctcgac
     3541 gctgaccagg aacgcccgcc tgaccggtcc cggcgcgcgg taggcgcccg gccgtttggc
     3601 agccagggag gcccagagtg acccctcgga cccggagcgg tgttacattg gcaccatgcc
     3661 ctccgggtcc gaggaatcac gcctcgtcag ggctgccgct tccggggacg gctctgcgtt
     3721 tgcgtctttg tacgagacgt acgaacggcc gatcttcaat tacgtgttgc ggttgctcgg
     3781 cgaccggcac gaggccgagg acgcgaccca ggacgccttc atcagggtca tggccaagct
     3841 gccggagctc gatctcgacc ggttcgagtt cgctccctac ctctacacag ccgccagaaa
     3901 cgccggttac gac
//
LOCUS       DAOQFI010000077         3857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10631_length_3857_cov_2.788269, whole genome shotgun sequence.
ACCESSION   DAOQFI010000077 DAOQFI010000000
VERSION     DAOQFI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10631_length_3857_cov_2.788269"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PLE93_02205"
     CDS             complement(<1..1430)
                     /locus_tag="PLE93_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HNH85891.1"
                     /translation="MSNGQDRTPYVDALLAFAQSDPGRFNVPGHQGGIGADPLLHQLV
                     GQTGLSDDIPGLIEGIDVGDPNPFQEAQALAAGAWGAKRTWFLINGASQGNQAMCLAV
                     AHMGGELVVQRNVHSSVIDGMIMAGLKPTFAAPELDPDLGVAHCLTPDDLAEALDRCP
                     DASAAVVVSPTYFGACADISGLAEVAHSRGVPLVADEAWGAHLHFHPDLPPSAIESGA
                     DLVISSTHKIVGSLTQSAMIHLATDRFDQAIIDRCISMTESTSPNSLLCGSLDAARRQ
                     AAIHGERLLGETIEVLVEAREEIRSIPGLDVLDERLLERKSVVGWDPLRLTIDVRGTG
                     STGYHIAELARDLAGVNLELASDTVAVAVFGMGTGTAQRARRLIDGVLTALEAIEAEP
                     VQEKPEFAPPPLWGDPAMTPRDAFLGKQEVVPFSEAEGRIAAEPLATYPPGIPNVLPG
                     ERLTRETLDYITDSVDHGGYVRGAADR"
     gene            1482..2051
                     /locus_tag="PLE93_02210"
     CDS             1482..2051
                     /locus_tag="PLE93_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85892.1"
                     /translation="MNLPSDTTRASRFIPAAFLCALSIALLAGCGGGSSEEDLSREGF
                     IADADAICAQYQPTSATLEQQFNQAIGNGELEDAAQDFEDQAADFSAMLDELEGLTAP
                     TADQTTVTQIIDLGRQRVDIAKEAADAIASGDKDAMISAGKKASILAGQYYQLTDGFG
                     FETCGSSGASGTTGTTGPAGSTGTTGATS"
     gene            complement(2080..3546)
                     /locus_tag="PLE93_02215"
     CDS             complement(2080..3546)
                     /locus_tag="PLE93_02215"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH85893.1"
                     /translation="MGYETRCLIGGEFVEGEGASIEVENPASEEIVATVGSASLEQLD
                     LAVAKATEAKASWGGMPAVDRAPLLHGIAAALREKTDELALLMTTEIGRPLSESADEV
                     EWCAACFDYYAEVARSEIGRVIPPIEASQFSVVVKEPHGVVGCIVPWNFPLLLLVWKV
                     APALAAGNTIVAKPSELTPLSTLAIAEVFSELPDGTVNLLAGAGDVGAGISGHPGIDC
                     VAFTGSVATGRKVGVAAASRNARVNLEMGGKDPFIVCDDIGTDGLEVAARGGAWAAFL
                     NAGQVCTSSERFYVMESVFDEYVSAFVEHTRSLVIGDPLESATDLGPMVSATQRGKAL
                     GQVEDAVAGGAELLTGGGNAGFGKGHYLEPCVLTGVDAAADLLREETFGPVAPIVPVK
                     DLDEAIALANGLDFGLGANIYTRDLKRAIRCVKEVRAGSVWINDPLTDNDAGPFGGFK
                     NSGIGRELGTEGLDAFREAKHAHIDTEIEVKDWWYPYG"
BASE COUNT          654 a         1294 c         1291 g          618 t
ORIGIN      
        1 cggtccgccg cacctcgcac gtagcccccg tggtcgaccg agtcagtgat gtagtcgagg
       61 gtctcgcggg tcagccgctc gccgggcagg acgttgggga tcccgggcgg ataggtggca
      121 agcggttccg cggcgatccg gccctctgct tccgagaagg gcacgacttc ctgcttgccg
      181 aggaacgcgt cccggggagt catcgcggga tcgccccaga gcgggggcgg cgcgaactcc
      241 ggcttctcct ggaccggttc ggcctcgatc gcctcgaggg cggtgaggac accgtcgatc
      301 aggcggcggg cccgctgggc ggtgcccgtt cccatgccga atacggcgac ggcgaccgtg
      361 tccgaggcca gttcgagatt gactccggcc aggtcccggg ccagctcggc gatgtggtac
      421 ccggtgcttc cggttccccg gacgtcgatg gtcagccgga ggggatccca gccgaccacg
      481 ctcttgcgct caagcagcct ttcgtcgagc acgtcgaggc cggggatcga gcggatctct
      541 tcccgcgcct cgaccagcac ttcgatcgtc tccccgagca accgctcccc atgtatggcg
      601 gcctgccgtc gggcggcatc gagcgaaccg caaagcagcg agttcgggct ggttgactcg
      661 gtcatcgaaa tgcagcggtc gatgatcgcc tggtcaaaac ggtcggtggc gaggtggatc
      721 atcgccgact gggtcaggct gccaacgatc ttgtgggtgg aggaaatgac caggtcggcg
      781 ccgctctcga tcgcgctggg cggcaggtca gggtgaaagt ggaggtgggc cccccaggct
      841 tcgtcggcaa ccaggggaac gccgcggctg tgggcgacct ccgccagtcc ggagatgtcg
      901 gcgcaggccc cgaagtaggt gggtgagacg accaccgccg cactcgcatc cgggcagcgg
      961 tccagcgcct cggccaggtc gtcgggagtc aggcaatgcg cgacgccgag gtccgggtca
     1021 agctccgggg cggcgaaggt tggcttcaac ccggccatga tcatgccgtc gatgaccgag
     1081 gagtggacgt tccgctggac caccagctcc ccgcccatgt gggcgaccgc caggcacatc
     1141 gcctggttgc cctgcgaggc cccgttgatc agaaaccagg tccgcttcgc gccccatgct
     1201 ccggcggcca gcgcctgggc ctcctggaac ggattcggat caccaacgtc gatgccctcg
     1261 atcagtccgg ggatgtcatc ggaaaggccg gtttgcccga ccagctggtg cagcagggga
     1321 tccgcaccga tcccgccctg atgacctggc acgttgaacc tgcccggatc actctgggca
     1381 aaggcgagca gcgcgtcgac ataaggagtt ctgtcctgac cgttagacat ggcaacgagt
     1441 ctattgacgg ccgcgccggg tccggagata ggctgccgat ggtgaatctc ccctctgaca
     1501 caacccgcgc ttcgcggttc atcccggccg cgtttctctg cgctctttcg atcgccttgc
     1561 tggccggctg tggcgggggc tccagtgaag aagacctctc gcgggagggc ttcatcgctg
     1621 acgctgacgc gatctgcgcc cagtaccagc caacttcggc cacactcgag caacagttca
     1681 accaggcgat cgggaacggc gagctcgagg atgccgccca ggactttgag gaccaggccg
     1741 cggatttctc cgccatgctc gatgaactcg agggcctgac cgccccgact gccgatcaga
     1801 ccacggtgac ccagatcatc gacctcggcc ggcagcgcgt cgacatcgcg aaggaggcag
     1861 ccgatgcgat tgcctcgggt gacaaggacg cgatgatctc cgcgggcaag aaggcttcga
     1921 ttctggccgg ccagtactac cagctgaccg acggcttcgg cttcgaaacc tgtggcagct
     1981 ccggggcctc gggcacgacc ggcaccaccg gaccggcggg cagcaccggc accaccggcg
     2041 ccacttcctg aaccggcagg gaagcggccc cgcccgggat cacccgtagg ggtaccacca
     2101 gtccttgact tcgatctcgg tgtcgatgtg ggcatgcttg gcttcgcgga aggcgtctag
     2161 accctcggtg ccgagctcgc ggccgatacc tgagttcttg aagccgccga agggtccggc
     2221 gtcattgtcg gtcagggggt cgttgatcca gaccgaacct gcccggacct ccttcacgca
     2281 gcggatcgcc cgcttaaggt cgcgggtgta gatgttcgcg ccgaggccga agtcgaggcc
     2341 gttggcgagc gcgatcgcct cgtcgaggtc cttgaccggc acgatcggcg cgaccggccc
     2401 gaaagtctct tcgcggagca gatcggccgc ggcgtcaacc ccggtcagca cacagggctc
     2461 gaggtaatgg cccttcccga atccggcatt tcctccaccg gtgagcagtt cggctccccc
     2521 ggcgacagcg tcctcgacct ggcccagcgc cttgccgcgc tgggtggcgg agaccatcgg
     2581 cccgaggtcg gtcgccgact ccagcggatc gccgatcact agcgacctgg tgtgctcgac
     2641 gaaggcgctc acgtactcgt cgaagaccga ctccatcacg tagaagcgct cggatgaggt
     2701 gcagacctgg ccggcgttca gaaaggccgc ccaggcaccg ccacgggcgg caacctcgag
     2761 cccgtcggtg ccgatgtcat cgcagacgat gaacgggtcc ttgccgccca tctcgaggtt
     2821 gacccgggca ttgcgggaag cagccgcgac gccgaccttc cggccggtgg caaccgaacc
     2881 ggtaaatgcg acgcagtcga tcccgggatg gccggagatc ccggcgccga catcgccagc
     2941 tcccgccagc aggttcaccg tgccgtccgg cagctccgaa aagacctcgg cgatcgcgag
     3001 ggtcgagagg ggagtcagct cggagggctt ggccacgatc gtgttgcccg cggccagggc
     3061 cggggccacc ttccagacca gcaagaggag ggggaagttc cacggcacga tgcagccgac
     3121 caccccgtgt ggctccttga ccacgaccga gaactgcgag gcctcgatcg gcgggatcac
     3181 gcgtccgatc tccgaacggg cgacctccgc gtagtagtcg aagcaggccg cgcaccattc
     3241 gacctcgtcc gccgactcgg aaagcggccg gccgatctcg gtggtcatca gcagcgcgag
     3301 ttcgtcggtc ttctcgcgca gggccgcggc gatcccgtgc agcagaggag cccggtcaac
     3361 ggccggcatc ccgccccagc tggccttcgc ctcggtcgcc ttggcgacgg cgaggtcaag
     3421 ctgctccagc gaagccgaac caacggtcgc gacgatctcc tcggaagccg ggttctcaac
     3481 ctcgatcgag gcgccttctc cctcaacgaa ctctccacca atcagacaac gagtctcata
     3541 tcccatgcgg gaaaactaac tgcagccggc aagcttcgcc ccgcttccag cttcaacccg
     3601 ccggacctgc aacacctggg ttgctcgcag gtgccgcacg tttaagcccg taacggtcct
     3661 gaatgcgcgg cacctcgaga cgtgccgggt gtgtttttcg aaggtgccgc aggtttgcca
     3721 ccggagaggt cgcaaacctg cgtcaccttg aagccggcag gggctggtga agggggagcg
     3781 ggttggaatc tcgggggggg ggattcgagg cgggagttat cggcaaatag cgcacccaac
     3841 tccgatttcg atccggg
//
LOCUS       DAOQFI010000078         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10657_length_3851_cov_3.772129, whole genome shotgun sequence.
ACCESSION   DAOQFI010000078 DAOQFI010000000
VERSION     DAOQFI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10657_length_3851_cov_3.772129"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLE93_02220"
     CDS             <1..180
                     /locus_tag="PLE93_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85894.1"
                     /translation="LTITAMTERAMSKIPPKDPAAELLHLPDEARPARTADTVGPARG
                     PGPSGSAESWTPLEP"
     gene            complement(255..1280)
                     /locus_tag="PLE93_02225"
     CDS             complement(255..1280)
                     /locus_tag="PLE93_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85895.1"
                     /translation="MIGLPTRTAVLPIPPKQLFFLALLAFAGLGMLISGCGSSESGDE
                     VARGAPSASDFPSAEGKTIEELLNDQTPSDKVVSPAAVVFEPGENRYPFGVFNVDRSQ
                     VQDADVAIYVAKSPQAPVEGPMPAEVVSLETKPAYRSKGAGGPGEANSFYLSHVKFDR
                     NGPWLAIAILKTADGFEAARVTPSPVVGQYPDVAQVGQQAPKVSTPTAEDVGGDLSKI
                     DTRQPPSSMHDDDFKDVVGKKPVVLIFATPALCQSRVCGPVVDVAEQVKDEVGDGVVF
                     IHNEVYNENDPSKGIRPQLTAFGLQTEPWIFLINKDGTIEKRIEGAVGVDELKSDVEQ
                     LKKKNGL"
     gene            complement(1452..2051)
                     /locus_tag="PLE93_02230"
     CDS             complement(1452..2051)
                     /locus_tag="PLE93_02230"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNH85896.1"
                     /translation="MAYEVPDLPYAYDALEPHIDEATMKVHHDKHHQAYVDKANAALE
                     GTEWADKPVEEVIANLSSLPADKQGPVRNNAGGHYNHSLFWEMLSPDGGGAPSGDLAA
                     AIDSAFGSFDDFKEQFKAGGIGQFGSGWVWLVKDGDGVAIVSTANQDSPLTDGKTPLL
                     GADVWEHAYYLKYQNKRPDYLDAFWNVVNWDYVAERFAS"
     gene            complement(2126..3661)
                     /locus_tag="PLE93_02235"
     CDS             complement(2126..3661)
                     /locus_tag="PLE93_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85897.1"
                     /translation="MVFSNGKRLIRIKADATDREVLTLKNSFVSSIDGLHDTALAGSP
                     DGKSVLFRREDWSDRPTGPQGLMSLMVDGGKPKLLVKQGSLSWIQAATFSADGSLIYF
                     TKTRYAGDWESYTLSVNSIRPDGTGLETIWSAPPVDLDDDEYSIGGITPAPDGRLLLT
                     FDYDTSDSHLDLLDPDTGEVTPLKAGAAWGSYSPDGSRIAFINMKQGYGALSVMNADG
                     TGETRIAPKVPGFIDRGPDWSADGSRIIFQSVRDGWVASYGEEVWAVEPDGSCLTRLT
                     NGAPESTAPVWLGTAGDSFEPGGCGDRAPNAIVETSIPKRVRGLDPPRFWMGPEWEGM
                     LYDDLIRIMNGVSFGYSDCTEFDYTQCSGYREVSTDAVCSYPFFPENYAGMRRVRGTV
                     IVRQNGDYRNEATVYTGGLSVDIQINHAKSFADFVEAARLIQPVSGPRLHRLEGPVFT
                     LGDVRTAHLVLRTKRQEGSFASLRRLGISPREARGYLRFARDLDRIGKYGTTRCRGFS
                     NLP"
     gene            3642..>3851
                     /locus_tag="PLE93_02240"
     CDS             3642..>3851
                     /locus_tag="PLE93_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNH85898.1"
                     /translation="MPFEKTTDALSTGGGVSARAGELTAITPKTKAILAVEAFGNPCH
                     MAEIASIAAKHEIPLIEDSCEGLGGA"
BASE COUNT          648 a         1245 c         1269 g          689 t
ORIGIN      
        1 ctgacgatca ccgcgatgac cgaacgggcg atgagcaaga tcccgcctaa ggatcccgcc
       61 gccgaactgc ttcacctgcc ggacgaggcc cgcccggccc ggaccgccga cacagtcggc
      121 ccggcccgcg ggcccggccc ctccgggtcg gccgaatcct ggactcccct cgagccctag
      181 accgcccgcg cggacagttc cgcgagtgcc cactttgcgc cacataccgg cacaaagtgg
      241 acactcggtg aaacctagag gccgttcttc ttcttgagct gctcgacgtc cgacttcagc
      301 tcgtcgacgc caaccgcgcc ttcgatccgc ttttcgatcg tcccgtcctt gttgatcagg
      361 aagatccacg gctcggtctg gaggccgaac gcggtcagct gcgggcggat gcccttgctc
      421 ggatcgttct cgttgtagac ctcgttgtgg atgaagacga ccccgtcgcc gacttcatcc
      481 ttgacctgct cggccacgtc cacgaccggt ccgcagaccc gggactggca gagcgccggg
      541 gtggcgaaga tcagcaccac cggtttcttg ccgaccacgt ccttgaaatc gtcgtcatgc
      601 atgctcgagg gcggctggcg cgtatcgatc ttcgagaggt cgccaccgac gtcttcagcg
      661 gtcggggtgc tgaccttcgg cgcctgctgg ccgacctggg ccacgtccgg gtactgcccg
      721 acgaccggac tcggtgtgac gcgggccgct tcgaaaccgt cggcggtctt gaggatcgcg
      781 atcgccagcc aggggccgtt gcggtcgaac ttcacatgcg agaggtagaa gctgttggcc
      841 tcgccagggc cgccggcacc cttcgagcgg taggcaggct tggtttccag tgaaacgacc
      901 tcggccggca tcgggccttc caccggcgcc tgcggagact tggcgacata gatcgccacg
      961 tcggcatcct ggacctggct ccggtccacg ttgaagacac cgaacggata ccggttctcg
     1021 cccggttcga acacgaccgc ggccggagag acgaccttgt cgctgggggt ctggtcattc
     1081 agcagctctt cgatcgtctt tccttcggcc gaaggaaagt cggaagccga aggagcgccg
     1141 cgggcaacct cgtctccgga ttcactgctg ccgcagccgg agatcaacat gccgaggccg
     1201 gcgaaggcaa gcagcgcgag gaagaagagc tgcttcgggg ggatgggcag gacagcggtc
     1261 cgggtgggaa ggccgatcat cggaaggatc ataagtagtc ggcgagcttc gccccgcttg
     1321 ggcgggtggg aagaacgtga acccacccac agaatcacaa aaagaaagcg gcccggacta
     1381 agccgggccg ccagtgaaac ccgccggccc acgacatcca ggagtggatg ggccggctct
     1441 ggttgggata actaggaggc gaagcgctcc gctacgtagt cccagttgac tacgttccag
     1501 aaggcgtcca ggtaatccgg gcgcttgttc tggtacttga ggtagtaggc gtgctcccag
     1561 acgtcggcgc cgagcagcgg ggtcttgccg tcggtcagcg gagaatcctg gttggcggtc
     1621 gagacgatgg cgacgccgtc gccgtccttg accagccaga cccagccgga gccgaactgg
     1681 ccgatgccgc cggccttgaa ctgctccttg aaatcgtcaa aggaaccgaa ggcggagtcg
     1741 atcgccgcgg caaggtcgcc gctcggggcg ccgccgccgt cgggcgagag catctcccag
     1801 aagagcgagt ggttgtagtg accaccggcg ttgttgcgga ccgggccctg cttgtcggcc
     1861 ggcagcgagg aaagattggc gatgacttcc tcgaccggct tgtcggccca ttcggtgcct
     1921 tcgagggcgg cgttggcctt gtcgacatac gcctgatggt gcttgtcgtg atgcaccttc
     1981 atggtcgcct cgtcgatgtg cggctcgagg gcgtcgtatg cgtagggaag atcgggaact
     2041 tcgtatgcca tcaggggact ccttgactgg tttcttgagg tttcgtgaca ggtacccgaa
     2101 ccctaacggt ccgacgcgct ccgcctcacg gcaggttgct gaatccccgg cagcgggttg
     2161 tgccgtattt gccgatccgg tcgaggtctc gggcgaagcg gaggtagccc cgggcctccc
     2221 gcgggctgat tcccagcctt ctcagcgagg cgaatgaacc ctcctgccgt ttcgtgcgga
     2281 ggaccaggtg cgccgttcgg acgtcgccga gggtgaagac gggtccctcc aggcggtgga
     2341 gccgcgggcc gctgaccggc tggatcaacc ttgccgcctc gacgaaatcg gcgaaggact
     2401 tcgcgtgatt gatctggatg tcaaccgaaa gcccgccggt gtagacggtt gcctcgttgc
     2461 ggtagtcccc gttctgcctg acgatcaccg ttccgcggac ccggcgcatc ccggcgtagt
     2521 tctcggggaa gaacggatag ctgcagaccg cgtcggtgct gacttcgcgg tagccggaac
     2581 attgtgtgta gtcgaactcg gtgcagtccg agtaaccgaa ggagacgccg ttcatgatcc
     2641 tgatcaggtc gtcgtagagc atcccttccc attccgggcc catccagaac cggggcgggt
     2701 ccagcccgcg cacccgtttg gggatgctcg tttcgacgat cgcattcggg gcccggtccc
     2761 cgcagccgcc aggttcgaag gagtcccctg cggtgccgag ccagaccggc gcggtgctct
     2821 ccggcgcacc gttggtcagc cgggtcaggc agctgccgtc cggttcgacc gcccagacct
     2881 cctccccgta gctggcgacc cagccatctc gcacgctttg aaagatgatc cggctgccgt
     2941 cggccgacca gtcgggcccg cgatcgatga agcccggcac cttcggggcg atccgcgtct
     3001 cgccggtgcc gtcagcgttc atcacactga gcgcgccata gccctgcttc atattgatga
     3061 aggcgatccg gctgccgtcg ggcgaatacg agccccaggc ggctccggcc ttgagcggcg
     3121 tcacctcgcc ggtgtccgga tcaagcaggt cgaggtggct gtcgctggtg tcgtaatcga
     3181 aggtgaggag caaccggccg tccggggcgg gcgtgattcc gccgatcgag tactcgtcgt
     3241 catcaagatc gaccgggggc gcggaccaga tggtttccag gccggttccg tcgggacgga
     3301 tcgagttgac gctcagggtg tagctctccc agtccccggc gtacctggtc ttcgtgaagt
     3361 agatcagcga accgtccgcc gaaaacgttg cggcctgaat ccagctcagg gagccctgtt
     3421 tgaccagcag cttcggcttg ccgccgtcaa ccatcagtga catcaggccc tgcgggccgg
     3481 ttggcctgtc agaccagtct tcccgccgga agagcaccga cttgccgtcg ggcgacccgg
     3541 ccagggcggt gtcgtgaaga ccgtcgatgc tgctgacgaa ggagttcttc agggtcagga
     3601 cttcgcggtc ggtggcgtcg gccttgatcc ggatcagccg cttgccgttc gagaagacca
     3661 ccgacgcctt gtcgaccggc ggtggggtca gcgccagggc aggcgagctg accgccatca
     3721 ctccgaaaac caaggccatc ctcgccgttg aagccttcgg caatccctgc cacatggccg
     3781 agatcgcctc catcgccgcc aaacacgaaa tccccctgat cgaagactct tgcgaaggtc
     3841 tcggcggcgc g
//
LOCUS       DAOQFI010000079         3846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10680_length_3846_cov_3.662358, whole genome shotgun sequence.
ACCESSION   DAOQFI010000079 DAOQFI010000000
VERSION     DAOQFI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10680_length_3846_cov_3.662358"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /gene="clpX"
                     /locus_tag="PLE93_02245"
     CDS             <1..829
                     /gene="clpX"
                     /locus_tag="PLE93_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNH85899.1"
                     /translation="DVENILLKLIQAADYDVKKAETGIIYIDEIDKITRKADNPSLTR
                     DVSGEGVQQALLKILEGTTASVPPQGGRKHPHQEFLTIDTTNILFICGGSFSELEKVI
                     ERRVDHKGIGFGAAITEQAHDDPGELFEQVLPEDLVEYGLIPEFIGRLPVVAALHQLD
                     QTDLVRILTEPKHALVRQFQRFFEFDDIELVFADDSLEAIADKALARETGARGLRSII
                     EDALLDVQFELPSREDVSKCVVTRESIEKGQSPMLVTEPSPVADPHDEDLFGEEQTA"
     gene            855..1649
                     /locus_tag="PLE93_02250"
     CDS             855..1649
                     /locus_tag="PLE93_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH85900.1"
                     /translation="MGAPPLDRDSSDLQVTWGGHATVLIEIDGVRLVTDPVLRSRVVH
                     LRRQVPDPGTAFIEGIDAVLISHLHFDHLDIPTLKRFDRGVKVIVPGGAGPMIRKHGF
                     REVIELMEGEATEVGPVTVLAVPADHDGRRHPFGMEIDAIGFVVEGSRRILFAGDTDL
                     HPRLGELADSLEVALLPIWGWGHRLGPGHMDPRGAAQAAATLRPKITVPIHWGTYFPI
                     GMKGIRGALLEKPVKAFEQQMEEIAPGLEIEVLRPGESLSRPAASS"
     gene            complement(1606..3666)
                     /locus_tag="PLE93_02255"
     CDS             complement(1606..3666)
                     /locus_tag="PLE93_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNH85901.1"
                     /translation="MEGLNEPETRRHPGKLFARILVVVLIDAAALLLIEAVLPGFDMH
                     GRFAALPTALGVGLVNALIWPVLSRFTLKLSVLTLGLWGLFLNAVLIGFALMAMPWVR
                     IEGPFEAIVISFGMAILTSLFSSLFAIDEDSTWYYNVVRTQLRRRGQVIETDEPGIVF
                     LEIDGLAHDILRRAMTNGNAPAMAAWVRDGSHKLEGWETDWSSQTGACQAGILHGNNH
                     DMPAFRWWEKETGRALVTNHPKDAAEMEARQSDGNGLLADDGASRANILSGDAPHSML
                     TMSTVMKRRGKIGQDYAAYFARPYGVIKTFIHTIAEIVRERRDNRRQERTGIEPKIER
                     SRVYAIMRAWATVIQLDLQISGVVGDILAGRPSIYTTFLAYDEVAHHSGIERPESLST
                     LAKVDREIDRIRRVVAEAPRPYHLVVLSDHGQSQGATFLQRYGVTLEEVVEKATGKEA
                     IGSGEEGEQNPRAYITASAEEASHEDGAIGSAATALARRDPNRDDLGLEGGPDEVAGG
                     EGKHEIPELSVMASGCLGLVTFPREPGRLTRERIDELYPGLLDTLVSHPGIGFVMVDS
                     EHGGVALGAEGLNYFEINDVEGVDPLAAFGPNTAAHVLRTHRFSNCPDIMINSTWWEE
                     MDEVAAFEELVGSHGGMGGTQSHPFVLHPVDLEWPEEPVIGAEAVHRILKSWLPAGTK
                     TLRA"
BASE COUNT          649 a         1261 c         1274 g          662 t
ORIGIN      
        1 ggacgtcgag aacatcctgc tgaagctgat ccaggccgcc gactacgacg tcaagaaggc
       61 ggagacgggg atcatctaca tcgacgagat cgacaagatc acccgcaagg ccgacaaccc
      121 gtcgctgacc cgtgacgtat ccggcgaagg cgtgcagcag gcgctgctga agatcctcga
      181 aggaaccacc gcctcggtac cgccgcaggg tggccgcaag catccgcatc aggaattcct
      241 gaccatcgac acgaccaaca tcctcttcat ctgcggcggc tcgttctcgg aactggagaa
      301 ggtgatcgag cgacgggtcg accacaaggg aatcggcttc ggcgcggcga tcaccgagca
      361 ggcccacgat gatcccggcg agctcttcga acaggtgctg cccgaggacc tggtcgagta
      421 cggcctgatc cccgagttca tcggccggct gccggtggtg gcggcgcttc atcagctcga
      481 ccagaccgac ctggtccgga tcctcaccga gcccaagcat gctctggtcc gccagttcca
      541 gcgcttcttc gagttcgatg acatcgagct ggtcttcgcc gacgattcac tcgaagcgat
      601 cgccgacaag gctctggccc gcgagaccgg cgcccggggg ctgcgctcga tcatcgagga
      661 cgccctgctc gacgtccagt tcgaactgcc ctcccgcgag gacgtgagca agtgcgtggt
      721 gacccgcgaa tcgatcgaga agggccagtc gccgatgctg gtgaccgaac cgagcccggt
      781 cgcggacccg cacgacgagg acctgttcgg cgaagagcag accgcctgac tcttcgccgc
      841 gggatactcc ctgcgtggga gcgccgcccc ttgaccggga ttcatccgat ctccaggtga
      901 cctggggcgg acacgccacg gtcctgatcg agatcgacgg ggtgcgcctc gtcaccgacc
      961 cggtgctccg cagccgggtg gtccacctgc gccggcaggt gcccgacccc ggaacggcct
     1021 tcatcgaagg catcgacgcg gtcctgatct cacatcttca tttcgaccat ctcgacatcc
     1081 cgaccctgaa gcgcttcgac cgcggggtga aggtaatcgt tccgggcggc gccgggccga
     1141 tgatcagaaa gcacggcttc cgcgaagtga tcgagctcat ggaaggggaa gcgaccgagg
     1201 tcggcccggt tacggtcctc gcagtgcccg ccgaccacga cggtcgccgc catcccttcg
     1261 ggatggagat cgatgcgatc ggcttcgtgg tcgaaggttc gcggcggatc ctgttcgccg
     1321 gtgacaccga cctgcatccg cggctcggtg aactggccga cagcctggaa gtggccctgc
     1381 tgccgatctg gggctggggt cacaggttgg gtcccggcca catggacccg cgcggggcag
     1441 cgcaggcggc cgcgaccctg aggccaaaaa tcaccgtgcc gatccactgg ggcacctact
     1501 ttccgatcgg catgaagggg atccgcgggg ctctgctgga aaagccggta aaggccttcg
     1561 agcagcagat ggaggagatt gcccccggcc ttgagatcga ggtgctcagg cccggagagt
     1621 ctttgtcccg gccggcagcc agctcttgag aatccggtga accgcttcgg ctccgatcac
     1681 cggttcttcc ggccactcga ggtcgaccgg atggagcacg aagggatggc tctgggtgcc
     1741 gcccatgccg ccgtgtgagc cgaccagctc ctcgaaggcg gcgacctcgt ccatctcctc
     1801 ccaccaggtg ctgttgatca tgatgtcggg gcagttgctg aaccggtggg tccgcaggac
     1861 atgggcagcg gtattcggcc cgaacgcggc cagggggtcg accccttcga cgtcgttgat
     1921 ctcgaagtag ttcaggccct cggcgccgag cgcgacgccg ccgtgttcgg aatcgaccat
     1981 cacgaagccg atcccgggat gtgacaccag ggtgtcgagc aggccggggt agagctcatc
     2041 gatccgttcg cgggtgagcc ggcccggttc gcggggaaag gtaaccagcc cgaggcaacc
     2101 ggaagccatc accgacagtt ccgggatctc gtgctttcct tcacctccgg cgacttcatc
     2161 cggtccgcct tcgagcccga ggtcgtcgcg gttcgggtcc cgccttgcca gcgcggtggc
     2221 ggcgctgccg atcgcaccgt cctcgtggct cgcctcctcg gcactggccg tgatgtaggc
     2281 gcgggggttc tgttcgcctt cctcgccgga gccgatcgcc tccttgccgg tcgccttctc
     2341 gaccacttcc tcgagcgtca cgccgtaacg ctgaaggaag gtagccccct gggactggcc
     2401 gtggtccgag aggacgacca ggtggtaggg ccgcggggcc tcggcgacga cccggcggat
     2461 ccggtcgatc tcacggtcga ccttggccag ggtggagagg gattcgggac gctcgatccc
     2521 ggagtggtgg gctacctcgt cgtaggcgag gaaggtcgtg taaatggagg ggcgaccggc
     2581 gaggatgtcc ccgaccacgc ccgagatctg aaggtcgagc tggatcaccg tggcccaggc
     2641 ccgcatgatc gcgtagaccc tcgagcgttc gatcttcggt tcgatcccgg ttctttcctg
     2701 gcgccggttg tcccgccgct cgcggacgat ctcggcgatc gtgtggatga aggtcttgat
     2761 caccccgtag ggacgggcga aataggcggc atagtcctgg ccgatcttgc cccggcgttt
     2821 catcaccgtg ctcatggtca gcatcgaatg cggggcatca ccggagagga tgttggcccg
     2881 gctggcgccg tcatcggcca acaggccgtt tccgtccgac tgccttgcct ccatctcggc
     2941 cgcatccttc ggatggttgg tcacgagggc ccggccggtt tccttctccc accagcggaa
     3001 ggcgggcatg tcgtggttgt tgccgtggag gatcccggcc tggcaggcgc cggtctggga
     3061 ggaccagtcg gtttcccagc cttccagctt gtggctgcca tcccgcaccc aggccgccat
     3121 cgccggggcg ttgccgttgg tcatcgcgcg ccggagaatg tcgtgtgcga ggccgtcgat
     3181 ctcgaggaag acgatgccgg gctcatcggt ctcgatcacc tggccgcggc gcctgagctg
     3241 ggtgcggacc acgttgtagt accaggtgct gtcctcgtcg atcgcgaaaa gcgaggagaa
     3301 gagcgaggtc aggatcgcca tcccgaagga gatcacgatt gcttcgaacg gtccctcgat
     3361 ccgcacccag ggcatcgcca tcagggcgaa cccgatcagc accgcgttca ggaaaagtcc
     3421 ccagaggccg agggtcagga cgctgagctt gagggtgaag cgggaaagga ccggccagat
     3481 caaggcgttg acgaggccga ccccgagtgc ggtgggcagg gcggcaaaac ggccgtgcat
     3541 gtcgaagccc ggcagaaccg cctcgatcag caaaagcgcg gccgcgtcga tcaggacgac
     3601 gaccaggatt cgggcgaaca gcttgccggg gtgccggcgc gtttccggct cgttcagtcc
     3661 ctccattgcc ggaactctcg caaaccgggc ggtccccggc aatcaccccg accggatgaa
     3721 cccctcagcg cctctttttc gccttcgaaa ccttcctgat cacccgcgtc acgaccttcg
     3781 accggttgcc ggccttgtca atcgcccgga tcgacaccgt gttctttccc ttgcggagcc
     3841 tggggg
//
LOCUS       DAOQFI010000080         3794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10934_length_3794_cov_3.327895, whole genome shotgun sequence.
ACCESSION   DAOQFI010000080 DAOQFI010000000
VERSION     DAOQFI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10934_length_3794_cov_3.327895"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PLE93_02260"
     CDS             <1..1312
                     /locus_tag="PLE93_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85902.1"
                     /translation="IVRREWGERAQAGAIWFAFAATISLFSGRLTFALGCLLAMAAVF
                     ASQRGLRIPALLLAGSVGLASPVAALFLACLGFAHFLAERPDRRGLELAGVSFLVAAI
                     VALLFPGGGDEPYVTSSFIPAIGLTLVSAWMVPPGQRLVRTGMLVYAGALVASYVLST
                     PMGGNVNRLGALLLGPVLLCVLAAEPLTPKLWRRGVLIILLPMIVWWQTGPVIRDLKL
                     TDDDPAVTQAFYQPLADALEPRLVREPARVEVVPIASHWEAARAAPEFPLARGWERQT
                     DRNYNPLFYADRLGPNRYRQWLDRLAVGYVALPADTELDYAGEKEAALIRSGLPFLRE
                     IPVGGDWRLFKVIGAQPMVARPARLTELDTDGFAVAAPIAGSFEVRIRSTPYWKVKSG
                     FGCVEPGRGDWTRVTLDRPGSLKVAADFSPGARFGSDRDCRPDR"
     gene            1343..1993
                     /locus_tag="PLE93_02265"
     CDS             1343..1993
                     /locus_tag="PLE93_02265"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH85903.1"
                     /translation="MESSSGPGGGQNGSRRPAQEFREQARNALIQSRLTPNAISMMGL
                     VGNLIAAGLVLHGDFFFAGIAFIIGSVMDTLDGRYSRMSGKGTLFGAFLDSTLDRIEE
                     GIVLAAVAWQFADSGDSVAAAMCVVVVLGSLMVSYTRARAEALGVECKVGIATRPVRV
                     VILSAGLVLGGEEVISGFDLLPIAIYVMAGLTAITTLQRVWHVRTELKKLDAAATE"
     gene            2174..3367
                     /locus_tag="PLE93_02270"
     CDS             2174..3367
                     /locus_tag="PLE93_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNH85904.1"
                     /translation="MTENGLNGNGNGASRGGDDKVRVAIVGVGNCANSFIQGVQYYKD
                     ADPADQVPGLMHVDLGGYHVKDIEFVAAFDIDQEKVGKDLSEAIWSGQNDTIKFADVP
                     NLGVTVERGMTLDGLGKYLKQVITKAPGPTADIVQILKDTKTDVLVCYLPVGSEDAVK
                     WYVEQAIAAGVGFVNCLPVFIASQDYWDGRFKEAGLPIIGDDIKSQVGATIVHRQLAR
                     LFHERGVEMQHTSQLNVGGNMDFFNMLERERLDSKKISKTNAVTSIMGTELEDRDVHV
                     GPSDYVPWLTDRKWAHIRLEGKSFGDVPLNVELKLEVWDSPNSAGVVIDAVRLIKLAL
                     NNGIAGQLDGPSSYLMKSPHNQRPDAEARQQTEDFIVENARGKSALPGTKPVAESAED
                     AKSEA"
     gene            3369..>3794
                     /locus_tag="PLE93_02275"
     CDS             3369..>3794
                     /locus_tag="PLE93_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH85905.1"
                     /translation="MTTGSALSIVQFTPFPWSERGGINEYTRRVSEELARRGHSVLIA
                     APSGGRGGVTETRAALAELADDPDALYRPGEEPRVLSMGGGVKIPRTGKRQAPVSVDV
                     GNRVEALLGTGRFDIVHVHDPFVPGFSSTALRHSFSLNVG"
BASE COUNT          584 a         1324 c         1228 g          658 t
ORIGIN      
        1 gatcgtccgg cgcgagtggg gtgagcgggc ccaggccggg gcgatctggt tcgccttcgc
       61 ggccacgatc agcctgtttt cggggcggct caccttcgcc ctcggttgcc tgctggccat
      121 ggcggcggtc ttcgcctcgc agcgcggcct gcgtatcccg gcgctgctcc tggccggctc
      181 ggtcggtctg gccagcccgg tcgccgccct tttcctggcc tgtctcggct tcgcgcattt
      241 cctggccgag cgacccgacc gccgcggtct ggagctggcc ggagtctcct tcctggtcgc
      301 cgcgatcgtc gcgctgctct ttcccggcgg aggcgacgag ccgtatgtca cttcgagctt
      361 cataccggcg atcggcctga ccctggtcag cgcctggatg gtccctccgg gccagcgcct
      421 ggtccgcacc gggatgctcg tctacgccgg cgccctggtc gcctcctatg tgctcagcac
      481 gccgatggga ggcaacgtca accggctcgg ggccctgctg ctcgggccgg tcctgctctg
      541 cgtgctggcg gccgagccgc tgaccccgaa actgtggcgc cgcggcgtgc tgatcatcct
      601 gctgccgatg atcgtctggt ggcagaccgg gccggtgatc cgggatctca agctgaccga
      661 tgacgatccg gcggtgaccc aggccttcta ccagcctctg gccgacgccc tcgagccacg
      721 cctcgtacgc gagccggcca gggtcgaggt ggtgccgatc gcctcccatt gggaagctgc
      781 ccgggcggcc ccggaattcc cgctcgcgcg cggctgggag cggcagaccg accgcaacta
      841 caacccgctc ttctatgccg accggctcgg tccgaaccgg tatcgccagt ggctcgaccg
      901 gctcgcggtc gggtatgtgg ccctgccggc cgacaccgaa ctcgactacg ccggggagaa
      961 ggaggcggcc ctgatccgct ccgggcttcc gttcctcagg gagatcccgg tcggcggcga
     1021 ctggcgactg ttcaaggtga tcggcgcgca gccgatggtc gcccgtccgg cccgcctgac
     1081 cgaacttgac accgacggct tcgcggtcgc ggcgccgatc gccggcagct tcgaggtgag
     1141 aatcaggtcg accccctact ggaaggtcaa gagcggattt ggttgtgtcg agccgggccg
     1201 gggtgactgg acccgggtca cgctcgatcg ccccggatcg ctcaaggtcg ccgccgactt
     1261 cagccccggg gcccggttcg gctccgaccg ggactgtcgc cccgaccggt gagccacggc
     1321 tccaacctct agagttcccc gcatggagtc ttcgtccggc ccgggaggcg gccagaacgg
     1381 ttcgcgcagg ccagcccagg aattccggga gcaggcccgc aacgctctca tccagtcccg
     1441 gctgaccccg aacgcgattt cgatgatggg gctggtcgga aacctgatcg ccgccggtct
     1501 cgtgcttcac ggcgatttct tcttcgccgg catcgctttc atcatcggtt cggtgatgga
     1561 caccctcgac ggccgttact cgcggatgtc gggcaagggc acgctgttcg gcgccttcct
     1621 cgattccacc ctcgaccgga tcgaagaggg catcgtgctc gccgcggtcg cctggcagtt
     1681 cgccgacagt ggtgacagcg tggccgccgc gatgtgcgtg gtggtcgtgc tcggatcgct
     1741 gatggtcagc tacacccggg cccgagccga ggcgctcgga gtcgaatgca aggtcggtat
     1801 cgccacccgg ccggttcgcg tcgtgatcct ttcggccggg ctggtcctcg gcggggagga
     1861 agtgatctcg ggctttgacc tgctgccgat cgccatttac gtgatggcgg gattgaccgc
     1921 gatcaccacc ctccagcggg tctggcatgt ccggaccgag ctgaagaagc tcgacgcggc
     1981 cgcgactgaa taggcggaca ggcccgaaat taatgcccgg cagcctgtaa ccggggccgt
     2041 tttgtggtgt cattaggggt ctacccggcc ccgcgcccgg atcaggcgtg gctttttgcc
     2101 cctgatcagc gtgcggaccg gtgcccaacc ggcaagagat gccaccccac ccacaggaga
     2161 ctttcgctta cccatgactg agaacggact taacggaaac ggcaacggcg ccagccgtgg
     2221 cggtgacgac aaggtacgag ttgccattgt cggcgtcggc aactgcgcca actccttcat
     2281 ccagggagtc cagtactaca aggacgccga tccggcggat caggttcccg gacttatgca
     2341 tgtcgacctc ggcggctatc acgtcaagga catcgagttc gtggctgcct tcgacatcga
     2401 tcaggagaag gtcggcaagg acctttccga agcgatctgg tcgggtcaga acgacacgat
     2461 caagttcgcc gacgttccca acctcggcgt cacggtcgag cgcggcatga ccctcgacgg
     2521 cctcggcaag tacctcaagc aggtcatcac caaggccccc ggcccgaccg ccgacatcgt
     2581 ccagatcctc aaggacacca agaccgacgt tctggtctgc tacctgccgg tcggctccga
     2641 agacgccgtc aagtggtacg tcgagcaggc gatcgccgcc ggcgtcggct tcgtcaactg
     2701 cctgccggtc ttcatcgcca gccaggatta ctgggacggc cgcttcaagg aagccggcct
     2761 gccgatcatc ggtgacgaca tcaagtccca ggtcggcgcg accatcgtcc accgtcagct
     2821 tgcccgcctc ttccacgagc gcggcgtcga gatgcagcac acctcgcagc tcaacgtcgg
     2881 cggcaacatg gacttcttca acatgctcga gcgcgagcgc ctcgactcga agaagatctc
     2941 gaagaccaac gccgtcacct cgatcatggg caccgagctc gaggaccgcg acgtccacgt
     3001 cggcccctcg gactacgtgc cgtggctgac cgaccgcaag tgggcccaca tccgcctcga
     3061 gggcaagtcc ttcggcgacg taccgctcaa cgtcgagctg aagctcgagg tctgggactc
     3121 gccgaactcg gccggggtcg tgatcgacgc cgtgcgcctg atcaagctcg ccctgaacaa
     3181 cggcatcgcc gggcagctcg atggcccctc ctcgtacctc atgaagtccc cgcacaatca
     3241 gcggccggac gccgaggccc gtcagcagac ggaggacttc atcgtcgaga acgctcgcgg
     3301 caagtcggct ctcccgggca ccaagccggt cgccgaatcg gccgaggacg cgaagtccga
     3361 agcctgattt gaccacgggt tctgcccttt cgatagttca gttcaccccg ttcccctgga
     3421 gcgagcgggg tggcatcaac gagtacacgc gccgggttag cgaagagctg gcccggcgcg
     3481 gtcattcggt gctgatcgct gccccttccg ggggccgcgg cggcgtcacc gagaccaggg
     3541 cggcactggc cgagctcgcc gacgaccccg acgccctcta ccgccccggg gaggaaccca
     3601 gggtcctttc gatggggggc ggggtgaaga tcccgcgcac cgggaagcgt caggccccgg
     3661 tttcggttga cgtgggcaac cgggtcgaag ccctgctcgg gaccggtcgc tttgacatcg
     3721 tccacgtcca cgaccccttc gtgcccggct tctcctcgac cgccctgcgc cattccttct
     3781 cgctcaacgt cggc
//
LOCUS       DAOQFI010000081         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_10983_length_3784_cov_3.405471, whole genome shotgun sequence.
ACCESSION   DAOQFI010000081 DAOQFI010000000
VERSION     DAOQFI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10983_length_3784_cov_3.405471"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLE93_02280"
     CDS             complement(<1..873)
                     /locus_tag="PLE93_02280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH85906.1"
                     /translation="MSQQSFADLGVSNAVVNSLRDRGIEAPFPIQRQVIEDILEGDDV
                     LVQSPTGSGKTLAFGVPLVDLVESTDPRPTALILAPTRELASQIVDEMEAICHARALR
                     IAAVYGGVGLQQQAKRAAKSHIIVACPGRLEDQLQRGAFDLENVRYLVLDEADRMLDM
                     GFKPAVDRIVRQIDDSRQTLFFSATLEGAAGKLAAAYTIDPKTHVNRTPKEKQGTIEH
                     RFIHVSHEAKVETLVGELESPDRGRTLVFVRTKHGADRLVKKLSKRSRQVRAVAMHGN
                     KSQNQRQRALADFED"
     gene            1176..3185
                     /locus_tag="PLE93_02285"
     CDS             1176..3185
                     /locus_tag="PLE93_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH85907.1"
                     /translation="MFVHKAAVVGGGTMGGQIAQAIAAADIPVVVKDVDQKFVDHAIE
                     EVRNVTAGQLASKVKKEKITQEQADARLEEVLANVTGTVTYDEFGDVDFVIEAVPEKM
                     AIKQAVFAELDAVTPGHAILSSNTSALSISEIGEATLRPDKVVGFHFFYPASVMPLVE
                     VVIGDDTSSETAQTSSNFAQAIGKSPITCGEVPGFVVNRILNSAVGEIWRAQEERGLS
                     IAKIDEGVGAANVSPMGPFILIDVLGLDTVYHVAEHLNDSYGERFYVHQGMKQLVADG
                     KLGAKTGGEGMYKDGQPNVPGDADADAQELTDLFLAKSLVEACLILEEGACSVRDIDW
                     GMMNGAGLNPMKGLFPPFWKADLEGLDVSLERIEKLNEKYGDERFPVPVTLKRLVAQG
                     RLGLSTGQGFYPYPQPDEGDQPEKVKLETRGNVAIAWLANPPMNAISPQVIEDLGTVW
                     EKVNANPEIGAMVIASSTGPIVYSAGADIKAFTKMDTEGGAALINNGHELLKSFGSGR
                     VATIAAVNSLAFGGGCELAMACDFRIAAESAVFGQPEIKLGIIPGFGGTQRLPRLVGW
                     GKALEMNTVGDPISSIEAFEAGLVNELVPDQELFDVALNWASRLAGQAPLAVEQVKQV
                     SFRGGDPDAGIDAEKQGFAKVFFSEDGKEGISAFLGKRKPDWQGK"
     gene            complement(3432..>3784)
                     /locus_tag="PLE93_02290"
     CDS             complement(3432..>3784)
                     /locus_tag="PLE93_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85908.1"
                     /translation="LWRAGSGKRRMITATLSRAKDPADVYRLLIPARRQIIANVTQLE
                     GDIVLTAFKPKTRSIYKPGKNVIVRSNRAYPKTEGILVRNLKKRPQMIWLAIPPSTSQ
                     TGNDSPYRLKVARR"
BASE COUNT          670 a         1221 c         1245 g          648 t
ORIGIN      
        1 atcctcgaag tcggccaggg cgcgctgccg ctggttctgc gacttgttgc cgtgcatggc
       61 gaccgcgcga acctgccggc tgcgcttgct cagcttcttg accagccggt cggcaccatg
      121 cttggtccgg acgaagacca gggtacggcc acggtccggg ctttcgagtt cgcccaccag
      181 ggtttctacc ttggcttcat gcgaaacgtg gatgaaacgg tgctcgatcg ttccctgctt
      241 ctccttcggc gtccggttga catgggtctt ggggtcgatc gtgtaggccg cggccagctt
      301 gccggcagca ccttcgagcg tggccgagaa gaagagggtc tggcggctgt cgtcgatctg
      361 gcggacgatc cggtcgaccg ccggcttgaa gcccatgtcg agcatgcggt cggcttcgtc
      421 gaggaccagg tagcgcacgt tttcaaggtc gaatgcgccg cgttggagct ggtcttccag
      481 ccggcccggg caggcgacga tgatgtggga cttcgcggcc cgcttggcct gctgctgcag
      541 gccgaccccg ccgtagacag cggcgatccg gagtgcgcgg gcgtggcaga tcgcttccat
      601 ttcatcaacg atctggctgg ccagctcgcg ggtcggggcg aggatcagcg cggtcgggcg
      661 agggtcggtg gattcgacca ggtcgaccag cggcacaccg aaggcaagcg tcttgccgga
      721 accggtgggg gactggacca gcacgtcatc gccttcgagg atgtcctcga tgacctgacg
      781 ctggatgggg aacggggctt cgattccgcg atcgcggagc gaattgacca cggcatttga
      841 cacgccgaga tcggcaaagg actgctggga catgcttttc aacttcctgt gaggggtaag
      901 ggggagatcc ggtaacaacc ccgacgccgg taagccgcgc gaaacaggaa ggaaacagaa
      961 cctcgatcgc gtccgtcagg gactgcgacc gcggcaaacg ataacaggcc ggataaagga
     1021 aacctctgca aaggacggat ttacgcggtg ctggcggcgt gtcgaccaca gtcccgatag
     1081 ccttattgac tcgcgagtca acttctcgcg cgcagtcggg tcagacaccc gcaaggctct
     1141 caactcacca agcccccgga ggaccaagaa acctcatgtt cgtacacaag gcagccgtcg
     1201 tcggcggtgg caccatgggc ggtcagatcg cccaggcgat cgccgcagct gacatcccgg
     1261 tcgtagtcaa ggatgtcgac cagaagttcg tcgatcacgc gatcgaggaa gtcaggaacg
     1321 tcaccgctgg ccagcttgcc agcaaggtca agaaggaaaa gatcacccag gagcaggccg
     1381 atgcccgcct cgaagaggtc ctggccaacg tcaccggcac cgtcacctac gacgagttcg
     1441 gtgacgtcga cttcgtgatc gaggccgtgc cggagaagat ggcgatcaag caggccgtct
     1501 ttgccgagct tgacgcggtc acccccggcc acgcgatcct ttcttccaac acctcggccc
     1561 tctcgatctc cgagatcggc gaggccacgc tgcgtcccga caaggtcgtc ggcttccact
     1621 tcttctaccc ggcctcggtc atgccgctgg tcgaggtcgt gatcggagac gacacctcgt
     1681 ccgagaccgc tcagacttcg tcgaatttcg cccaggcaat cggcaagtcc ccgatcacct
     1741 gcggcgaggt ccccggattc gtcgtcaacc gcatcctcaa ctccgcggtc ggcgagatct
     1801 ggcgcgccca ggaagagcgc ggcctctcga tcgccaagat cgacgaaggc gtcggcgccg
     1861 cgaacgtttc cccgatgggc ccgttcatcc tgatcgacgt cctcggtctg gacaccgtct
     1921 accacgtcgc cgagcacctg aacgactcct acggcgagcg tttctacgtc caccagggca
     1981 tgaagcagct ggtcgccgac ggcaagctcg gagccaagac cggcggcgaa ggcatgtaca
     2041 aggacggcca gccgaacgtt cccggcgacg ccgacgccga cgcacaggag ctgaccgacc
     2101 tcttcctcgc caagtctctg gtcgaggcct gcctgatcct cgaggagggt gcctgcagcg
     2161 tccgcgacat cgactggggc atgatgaacg gcgccgggct gaacccgatg aagggtctct
     2221 tcccgccgtt ctggaaggcc gacctggaag gtctcgacgt ttcgctcgag cggatcgaga
     2281 agctgaacga gaagtacggc gacgagcgct tcccggtgcc ggtcaccctc aagcgcctgg
     2341 tcgcccaggg ccgcctcggc ctcagcaccg gtcagggctt ctacccctac ccgcagcccg
     2401 acgagggcga tcagcccgag aaggtcaagc tggagacccg gggcaacgtc gcgatcgcct
     2461 ggctggccaa cccgccgatg aacgcgatct cgccccaggt catcgaagac ctcggcaccg
     2521 tctgggagaa ggtcaacgcc aatcccgaaa tcggcgccat ggtgatcgcg tcctcgaccg
     2581 gtccgatcgt ctactcggcc ggtgccgaca tcaaggcgtt caccaagatg gacaccgagg
     2641 gtggcgccgc gctgatcaac aacggccacg agctcctcaa gagcttcggt tcgggccgcg
     2701 ttgcgaccat cgccgccgtc aactcgctcg ccttcggcgg cggttgcgag ctcgcgatgg
     2761 cctgtgactt ccgcatcgcc gcggaatccg cggtcttcgg ccagccggag atcaagctgg
     2821 gcatcatccc cggcttcggc ggaacccagc gtctgccccg tctggttggc tggggcaagg
     2881 ccctcgagat gaacaccgtc ggtgacccga tctcctcgat cgaggcgttc gaggccggtc
     2941 tggtcaacga gctcgtgccc gatcaggagc tcttcgacgt cgccctgaac tgggcgagcc
     3001 gtctggccgg tcaggccccg ctcgcggtcg agcaggtcaa gcaggtcagc ttccgtggcg
     3061 gcgacccgga cgccggcatc gacgccgaga agcagggctt cgccaaggtc ttcttctccg
     3121 aggacggcaa ggaaggcatc tccgccttcc tcggcaagcg caagcccgac tggcagggca
     3181 agtaggaact gcgtcgagaa cccactttgc gccacatacc ggcgcaaagt gggttctcgg
     3241 tgaacccgcg caagggttca cagcaacctg cggcaccggt cgcatcgatt gtttccgggt
     3301 agccgcgggg ccgggtgagc gtgcccggga tactcaggaa gagggcggct gaatgccgcc
     3361 cgaagtgttt gccgcttccc gggcacgctc acccgacccc gcggcgtagc caccagggtc
     3421 cctcacggga gtcagcggcg ggcgaccttg aggcggtagg gggagtcgtt gccggtctgg
     3481 ctggtcgagg gggggatcgc gagccagatc atctggggcc gcttcttgag gttgcggacc
     3541 aggatgcctt cggtcttcgg gtaggcccgg tttgagcgga cgatcacgtt cttgccgggc
     3601 ttgtagatcg acctggtctt cggcttgaat gcggtcagga cgatgtcccc ttcgagctgg
     3661 gtcacgttgg cgatgatctg gcgtcgcgcg gggatcagca gccggtagac atcggccgga
     3721 tccttggccc tcgagagggt cgcggtgatc atccggcgct tgccggagcc cgcccgccag
     3781 agat
//
LOCUS       DAOQFI010000082         3770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11046_length_3770_cov_3.120861, whole genome shotgun sequence.
ACCESSION   DAOQFI010000082 DAOQFI010000000
VERSION     DAOQFI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11046_length_3770_cov_3.120861"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLE93_02295"
     CDS             <1..479
                     /locus_tag="PLE93_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85909.1"
                     /translation="DDRRSVDLAPGYSSSQTMADWAASRPGPDPTPTVQKLKVSGPVS
                     AKGTKATVKVSCPASAASCAKARIDIHGAPKNQKARGAGVLIGMVTGVTVNPGKSKTV
                     SIPMTVAARNLFKDQKGKKGKKGKKGLKSLAVQVVSTSTAGESVLKINLKRTGKVK"
     gene            complement(486..1100)
                     /locus_tag="PLE93_02300"
     CDS             complement(486..1100)
                     /locus_tag="PLE93_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNH85910.1"
                     /translation="MFDMDGVLIDTQAAVHDAYTAWAGENGLDPQEVLAIIHGRRTVE
                     VAQIFDVDEDVEAEADRIEKAIAERARAEDGIEPACELYRSLDRGRVAVATSARLETA
                     LTNLRVLGLEHPEVLVTGQDVESGKPSPDPYLLAAERLGVEPAECVVVEDAPAGIKAG
                     KAAGTFVVALTTTHEAIDLGEADRVIAPGQLEDLFTSAEPSSGA"
     gene            complement(1127..2299)
                     /gene="sufD"
                     /locus_tag="PLE93_02305"
     CDS             complement(1127..2299)
                     /gene="sufD"
                     /locus_tag="PLE93_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNH85911.1"
                     /translation="MASAELAVDAPAWLVERREKGAALVETIELPHAKTKGWEFTDIS
                     RLDLDSYENSPAEVTISGGSDEIIVLPLSEALASHSDLLAEKLGSLVSIEDPFVARNE
                     ATLNDGVLVYVPKNVECEEAIKVDLKLDTDGAAVNWRTLVVLEEGAKAEVWEHYGSAS
                     DETDALLNTVVELVVGQAANLHFVATQDLSEKAWLFSTQRGEVQRDGSLDWATLGFGG
                     GGGKVRMTTNLAGPGSDAKVTGGYAGGNSQHIDYDTLQEHAAEHTTSDLAFRGVLADK
                     SSAVWRGMIKVDEGAQQTDAFQECRNMLLSTDAHADAIPGLEILADDVACTHAAAIAQ
                     VDKDQLFYLESRGLSEETSRSLVVEGFLQSLVERLGKGPVRDEIAERLETRLAEIL"
     gene            complement(2292..3725)
                     /gene="sufB"
                     /locus_tag="PLE93_02310"
     CDS             complement(2292..3725)
                     /gene="sufB"
                     /locus_tag="PLE93_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNH85912.1"
                     /translation="MATAEVLAEKEKVQGINADYAEKFGFSDPETGYAYKAPKGLTRE
                     IVEQISEYKDEPQWMRDFRLKALEIFESKPTPQWGADLDQIDFDDIHYFVRASEKNSR
                     DWSEVPEDIKNTFDRLGIPEAEQKFLSGVGAQYESEVVYHQVNEKLEAQGVIFTDMDT
                     ALREHEDIIKEYWATVIPANDNKLAALNSAVWSGGSFVYVPKGVKVEMPLQAYFRINT
                     ENMGQFERTLIIAEEDSDVHYIEGCTAPVYSTDSLHSAVVEIIAKPGARVRYTTVQNW
                     SQNVFNLVTKRAIAEERATMEWVDCNLGSKVTMKYPAIYLLGEGAHGEVLSIAFAGEG
                     QHQDAGAKIIHGAPNTTSSVFSKSISKDGGRSTYRGLLEVADGATGTRSKVVCDALLL
                     DEKSQSDTYPTIRIGESDADMGHEATVSKVGDDMLFYLQSRGLSEEEASKMIVNGFIE
                     PVTKELPMEYAVELNRLIELQMEGSIG"
BASE COUNT          610 a         1185 c         1263 g          712 t
ORIGIN      
        1 cggatgaccg ccggtcggtt gacctcgctc ccggttacag cagctcgcag accatggctg
       61 actgggctgc gagccggccc ggtcccgacc cgaccccgac cgttcagaag ctgaaggtca
      121 gtggtccggt ctcggccaag ggcaccaagg ccacggtcaa ggtcagctgc ccggccagcg
      181 ccgcctcctg cgccaaggcc cggatcgaca tccacggcgc ccccaagaac cagaaggccc
      241 gcggggccgg ggtgctgatc ggaatggtca ccggcgtcac ggtcaacccc ggcaagtcga
      301 agacggtctc gatcccgatg accgtcgcgg cccgcaatct cttcaaggac cagaagggga
      361 agaagggcaa gaagggcaag aaggggttga agagcctcgc ggttcaggtg gtcagtacca
      421 gcaccgccgg ggagtcggtg ctgaagatca acctcaaacg gaccggcaag gtcaagtaga
      481 gcgcttcagg cgccgcttga aggctcagcc gatgtgaaca ggtcttcgag ttgcccgggg
      541 gcgatcaccc ggtcggcttc gcccaagtca atcgcttcgt gggtggtggt caatgcgacc
      601 acgaaggtgc cggcggcctt tcccgccttg attccggccg gcgcgtcctc gaccacgacg
      661 cattcggccg gctcgacgcc cagccgctcg gcagcgagca ggtaggggtc cggggagggc
      721 ttgccggatt cgacatcctg gccggtgacc agaacctcgg gatgctcgag gccgagcacc
      781 cgcaggttgg tgagggcggt ttcgagacgg gccgaggtgg cgacggcaac ccggccgcgg
      841 tcaagggagc ggtagagctc gcaggccggt tcgatcccgt cttcggcccg ggcccgctcg
      901 gcgatcgcct tctcgattcg gtccgcttcc gcttcgacgt cttcgtccac gtcaaagatc
      961 tgcgcgacct cgacggtacg gcggccgtga atgatggcga ggacttcttg cgggtcgagc
     1021 ccgttctccc cggcccaggc ggtgtatgcg tcgtgaacgg ccgcctgggt gtcgatcagc
     1081 accccgtcca tgtcgaagat gacggtggtg atttcaggcg gcagcttcag aggatctcgg
     1141 ccaggcgggt ttccagccgc tcggcgatct cgtcccggac ggggcctttg ccgagtcgct
     1201 cgaccagcga ctgaaggaaa ccttcaacca ccaggctgcg ggaggtttcc tcgctgaggc
     1261 cgcggctttc caggtagaag agctggtcct tgtcgacctg ggcgatcgcc gccgcatggg
     1321 tgcaggcgac gtcgtcggcg aggatctcga gcccggggat cgcgtccgcg tgagcgtcgg
     1381 tggagagcag catgttgcgg cactcctgga aggcgtcggt ctgctgggcg ccttcgtcga
     1441 ccttgatcat gccgcgccag acagccgagg acttgtcggc cagaacgccg cggaaggcca
     1501 ggtccgaggt ggtgtgttcg gccgcatgct cctgcagcgt gtcgtagtcg atgtgctgcg
     1561 agttgccgcc ggcgtatccg ccagtgacct tggcatcgga gccaggcccg gccaggttgg
     1621 tggtcatgcg gaccttgccg ccgccaccgc cgaaaccgag ggttgcccag tcgagcgagc
     1681 cgtcccgctg gacctcgccc cgctgggtcg agaagagcca ggccttctcg gaaaggtcct
     1741 gtgtcgcgac gaagtgaagg ttggcggcct ggccgacgac cagttcgacc acggtgttga
     1801 gcagtgcgtc ggtctcgtcg gaggcggagc cgtagtgctc ccagacctcg gccttggcgc
     1861 cttcctcgag cacgaccagg gtgcgccagt tcaccgcagc gccgtcggtg tcgagcttca
     1921 gatcgacctt gatcgcttcc tcgcactcaa cgtttttcgg cacgtagacg agcacgccgt
     1981 cattcagggt ggcctcgttg cgggccacga aggggtcctc gatcgagacc agcgaaccaa
     2041 gcttttcggc gagcagatcg gagtgggagg ccaacgcctc gctcagcggc aggacgatga
     2101 tttcgtcgct gccaccgctg atcgtcacct cggccggcga gttctcgtag gagtcgagat
     2161 cgagccgcga gatgtcggtg aactcccagc ccttggtctt ggcgtggggg agttcgatcg
     2221 tctcgaccag ggcagcgccc ttttcccgcc gctcgacgag ccaggcgggt gcgtcaactg
     2281 ccagctcggc gctagccaat tgaaccctcc atctgcagct cgatcagccg gttcagttcg
     2341 accgcgtact ccatcggcag ttccttggtg accggttcga tgaacccgtt gacgatcatc
     2401 ttcgaagctt cttcctcgga gagcccgcga ctctgcaggt agaagagcat gtcgtcgccg
     2461 accttggaaa cggtggcctc gtgacccatg tcggcatcgg actcgccgat ccggatcgtc
     2521 gggtaggtgt ccgactggct cttctcgtcg agcagcagcg cgtcgcagac caccttcgag
     2581 cgggtgccgg tcgcaccatc ggcgacctcg agcagtccgc ggtaggtgga acggccgccg
     2641 tccttggaaa tcgacttcga gaagaccgac gaggtcgtgt tcggggcacc gtggatgatc
     2701 ttcgcgccgg cgtcctgatg ctggccttcg cccgcgaagg cgatcgacag cacttcgccg
     2761 tgggcgcctt cgccgagcag gtagatcgcc gggtacttca tggtgacttt cgaaccgagg
     2821 ttgcagtcga cccattccat ggtcgcccgc tcctcggcga tcgcccgctt ggtgaccagg
     2881 ttgaagacgt tctgcgacca gttctggacg gtcgtgtagc ggacccgggc accgggcttg
     2941 gcgatgatct cgaccaccgc ggagtgaagc gagtcggtcg agtagaccgg ggcggtgcag
     3001 ccctcgatgt agtggacgtc ggaatcttct tcggcgatga tcagggtccg ctcgaactgg
     3061 cccatgttct cggtgttgat ccggaagtag gcctggagcg gcatctcgac cttcacgccc
     3121 ttcggcacgt agacaaagga gccgccggac cagaccgcag agttgagcgc ggccagcttg
     3181 ttgtcgttgg ccgggatcac ggtcgcccag tactccttga tgatgtcctc gtgctcgcgg
     3241 agcgcggtgt ccatgtcggt gaagatcaca ccctgcgctt cgagcttctc gttcacctgg
     3301 tggtagacga cctcggattc gtactgcgca ccgacgccgg agaggaactt ctgctcggct
     3361 tcggggatgc ccaggcggtc gaaagtgttc ttgatgtcct cggggacctc ggaccagtcg
     3421 cggctgttct tctcggaggc gcggacgaag taatggatgt catcgaagtc gatctggtcg
     3481 aggtcggcgc cccactgcgg ggtcggcttg ctctcgaaga tctccagcgc cttgagccgg
     3541 aagtcccgca tccactgcgg ctcgtccttg tactcggaga tctgctcgac gatctcccgg
     3601 gtcaggccct tcggggcctt gtaggcgtag ccggtttcgg gatcgctgaa gccgaacttc
     3661 tcggcgtagt cagcattgat cccctggacc ttctcttttt cggcgagtac ttcggcagta
     3721 gccacgttca aacctccagc gtgatggtgt cgtcgattac ctcgacagga
//
LOCUS       DAOQFI010000083         3763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11086_length_3763_cov_3.652373, whole genome shotgun sequence.
ACCESSION   DAOQFI010000083 DAOQFI010000000
VERSION     DAOQFI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11086_length_3763_cov_3.652373"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLE93_02315"
     CDS             <1..214
                     /locus_tag="PLE93_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85913.1"
                     /translation="AQAQDLTEKTRNRAQGLVDEVTRRGSDARGTLDGLRLVSRDEMR
                     QLEERIDEISSRLAELERKSKAQPEG"
     gene            266..1282
                     /locus_tag="PLE93_02320"
     CDS             266..1282
                     /locus_tag="PLE93_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH85914.1"
                     /translation="MSATKRIVIAGVSTRWGSELARTLQRRPGVGEIIGIDSAPPTSD
                     LGRTEFVEADIRNPVISRILPSLEPDVVVHCGIVWYPEKGRPSRALHDINVIGTLQLL
                     AACEKTSGLQALIARGSAAIYGSAPGAPAFFTEDMARRLPLVTRFQRDIGELEGYFDN
                     FARRRPEITCCMLRFGVEVGPGLDTPFNRYLNLPVVPTRMGFDPRLQLLHVDDATGAL
                     AAATLNPVRGAVNVAPDGAISLSRLLRLCGKSAVGVPPVLVGVINRRIGNYLGSADLY
                     RDGELLLRYGRGCDNRRLREEVGFELAFDSEGTARDFARTSQGIQALFPSPHPGSPVR
                     SVSR"
     gene            1279..2154
                     /locus_tag="PLE93_02325"
     CDS             1279..2154
                     /locus_tag="PLE93_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH85915.1"
                     /translation="MNRPAAPTPEAVLEWAGTVQSTSDLILRRLAGEYHEDAWGFDEE
                     FTEAVFPLFEFLYSTWWRVQVEGAANVPGHGRALLVANHSGAIFPFDGAMIATAIQKE
                     HPLPRWVKAMVLNWAFDLPFVSYILRRLGGVAANPANLGRLLEEDQPVMVFPEGQKGF
                     GKSFADRYRLQRFGRGGFVEVALETGSPIVPVAVVGAEEIYPKLGESKPLARAIGSPY
                     VPVTPTFPWLGPLGLLPLPSRWRIEFLEPIDLSGYGPGSSEDRSLVFDLSEQVREQIQ
                     EALYRGLVKRGPAFL"
     gene            2449..>3763
                     /locus_tag="PLE93_02330"
     CDS             2449..>3763
                     /locus_tag="PLE93_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNH85916.1"
                     /translation="MSVKRIGIASLLAMFAALVVPGMASAAPVTQELFDAKSAELNGT
                     FIALACVLVLLMQCGFMFLEIGFSRMKNAGAGVMKIFVNFSVCTLAFWAVGAAIIGYG
                     NDFIGTHGFFYHFGSMVDDGSGTGTLVAVDSSAVMYMLYSFAFCAVSLAIVWGTTLER
                     IKFAPYIIYAAVFGAVIYPMVGHSVWGGGLLTDIGGKSVMDFAGSSVVHLTGAVGGFV
                     ALLLLGARKGKYAADGSPRAIPGHSMPLVGLGIIILWIGWFGFNGGSTLGTDLSFFGE
                     VMWNTQIAAAAGVIGAMLFTYMKTKSLDVGMAGNGAIAGLVAITAPCGFVETWTAPII
                     GFIAGGVVVIGVLTIEKYLDDPIGALSAHGLAGIWGTLAVGIFASPRLTISAPGIWYG
                     IFGDADFGATLGQLGVQALAVVATFTFVFAASYLVFGIIKATIGLR"
BASE COUNT          587 a         1265 c         1199 g          712 t
ORIGIN      
        1 ggcccaggct caggatctca ccgagaagac gcgaaaccgg gcccaggggc tggtcgacga
       61 ggtgaccagg cggggatccg atgcccgggg gacgctcgac gggcttcgcc tggtcagccg
      121 cgatgagatg cgccagctgg aggagagaat cgacgagatt tccagtcggc tagccgaatt
      181 ggagcgtaag tcaaaggctc aacctgaggg ttagagttat tcgatttggc cccgattttg
      241 gccagatccc ggtccggagt tcaaattgag tgcgacaaaa cggatagtca tcgccggagt
      301 ttcaacccgc tgggggtcgg aactggcgcg gacgctgcag cgccggcccg gggtcggcga
      361 gatcatcggc atcgattcag ccccgcccac ctccgatctc ggccggaccg agttcgtcga
      421 ggcggacatc cgcaacccgg tcatctcccg catccttccc tcgctggaac cggacgtggt
      481 ggttcattgc gggatcgtct ggtacccgga aaagggccgg ccctccaggg cactccatga
      541 catcaacgtg atcggcaccc tgcagctgct cgccgcgtgc gaaaagactt ccggtctcca
      601 ggccctgatc gcccggggtt ctgccgcgat ctacggttcc gctcccggcg ccccggcctt
      661 cttcaccgag gacatggccc gccgcctgcc cctggtcacc cgcttccagc gcgacatcgg
      721 cgagctcgag ggctatttcg acaatttcgc cagaaggcgc cccgagatca cctgctgcat
      781 gctccgcttc ggggtcgagg ttggccccgg cctcgacacc cccttcaacc gttacctgaa
      841 cctgccggtg gtgcccacgc gcatgggttt cgacccccgg ctgcagctgc ttcacgtcga
      901 cgatgcgacc ggggcgctgg cggcggcgac cctgaatccg gtgcggggag cggtcaacgt
      961 cgcccccgac ggggcgattt cgctcagccg cctgctgcgg ctttgcggaa agagcgcggt
     1021 cggcgtgccg ccggtattgg tcggcgtgat caaccgccgg atcggcaact acctcggcag
     1081 cgccgatctc taccgcgacg gcgagctcct gctccgttac ggccgcggct gcgacaaccg
     1141 caggctgcgg gaggaggtcg ggttcgagct cgccttcgac tcggagggaa cagcccgcga
     1201 cttcgcccgc acctcgcagg gcatccaggc cctctttccg tcaccccatc cgggttcccc
     1261 ggtcaggagc gtttccaggt gaaccgcccc gccgccccga ccccggaggc cgtgctcgag
     1321 tgggccggaa cggtccagtc cacctcggat ctgatcctcc gccggctggc cggcgagtac
     1381 cacgaggatg cctggggctt cgacgaagag ttcaccgaag cggtcttccc gctcttcgaa
     1441 ttcctctact cgacctggtg gcgggtccag gttgaagggg cggccaacgt gccggggcac
     1501 ggccgggccc tgctggtcgc caaccattcc ggcgccatct tcccctttga cggcgcgatg
     1561 atcgccaccg cgatccagaa ggagcacccc ttgccccgct gggtcaaggc gatggtgctc
     1621 aactgggcct tcgacctgcc ctttgtcagt tacatcctgc gccggctcgg cggggtggcc
     1681 gccaacccgg caaacctcgg acggctgctc gaggaggatc agccggtgat ggtcttcccc
     1741 gaaggccaga agggcttcgg aaagtcattc gccgaccgtt accggctgca gcgcttcggc
     1801 cgcggcgggt tcgtcgaggt tgcgctcgag accgggtcgc cgatcgtccc ggtcgcggtg
     1861 gtcggggccg aggagatcta tccgaaactc ggcgagtcaa agccgctggc gcgagcgatc
     1921 ggttctccct acgtgccggt taccccgacc ttcccctggc tgggaccgct cggcctgctg
     1981 cctttgccct cgcgctggcg gatcgagttc ctcgagccga tcgacctctc cgggtacgga
     2041 ccgggttcga gcgaggaccg ctcgctcgta ttcgatctct ccgagcaggt gcgggagcag
     2101 atccaggaag ccctgtatcg cggcctggtc aaacgcggcc ccgcattcct ctagcccgcg
     2161 gccgtgaagt ccgggggccg gcggcccagt tgcctggccg ggtgtcccgg cggacaaaac
     2221 ggccgccgat ccagtcgccc gagtccgctc acagacccga atctcaacta cgactcggct
     2281 ctgtagagcc atttaccttc gatttaacaa tcgaacaacc gggatggggt catttcctcc
     2341 gagtggccaa aaagcgctcc ccctcccgtt tctaaattgg cccggtcagc taaacgctat
     2401 ttgcaaccgg tgggtctggg cccggcggaa acaggaggaa tgactcgtat gagtgtcaag
     2461 agaatcggca tagcctcgct tctggcgatg tttgccgccc tggtggtgcc agggatggcg
     2521 agtgcggcgc cggtcacaca ggaactgttc gacgccaagt cggccgagtt gaacggaacc
     2581 ttcatcgcgc tcgcctgcgt gctcgtgctg ctcatgcagt gcggcttcat gttcctggag
     2641 atcggtttct cccgaatgaa gaacgcaggc gccggagtga tgaagatctt cgtcaacttc
     2701 tcggtctgca ccctcgcctt ctgggcggtc ggggccgcaa tcatcggtta cggcaacgac
     2761 ttcatcggaa cccacgggtt cttctaccac ttcggttcga tggttgatga cggcagtggc
     2821 acaggcacgc tggtcgccgt cgattcgagc gcggtcatgt acatgctgta cagcttcgcg
     2881 ttctgcgcgg tctcgctcgc catcgtctgg ggcaccaccc tggagcgcat caagttcgct
     2941 ccgtacatca tctacgccgc agtcttcgga gccgtgatct acccgatggt cggtcactcg
     3001 gtctggggcg gcggcctgct caccgacatc ggtggcaagt cggtgatgga cttcgccggt
     3061 tcctcggtgg tccacctcac cggagcggtc ggcggattcg tcgcactgct cctgctgggt
     3121 gccagaaagg gcaagtacgc ggccgacgga tcaccccgcg cgatccccgg acactcgatg
     3181 ccgctggtcg gcctcgggat catcatcctg tggatcggct ggttcggatt caacggcggc
     3241 tcgaccctgg gcaccgatct ctccttcttc ggcgaggtca tgtggaacac ccagatcgct
     3301 gctgcggcag gcgtgatcgg cgcgatgctc ttcacctaca tgaagaccaa gtcgcttgac
     3361 gtcggcatgg ccggcaacgg cgcgatcgcc ggcctcgtcg cgatcaccgc gccctgcggt
     3421 ttcgtcgaga cctggacggc cccgatcatc ggcttcatcg ccggtggcgt cgtggtcatc
     3481 ggcgtcctga cgatcgagaa gtacctcgac gatccgatcg gtgccctctc ggcccacggt
     3541 ctggccggca tctggggaac cctcgcggtc ggcatcttcg cctcgccgcg actgaccatc
     3601 tccgcgccgg gcatctggta cggaatcttc ggtgacgccg acttcggcgc gaccctgggt
     3661 cagctcgggg ttcaggcgct ggccgtcgtt gcgacgttca cattcgtgtt cgccgcttcg
     3721 taccttgtgt tcggaatcat caaggcaacc atcggcctcc gtg
//
LOCUS       DAOQFI010000084         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11135_length_3755_cov_3.773243, whole genome shotgun sequence.
ACCESSION   DAOQFI010000084 DAOQFI010000000
VERSION     DAOQFI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11135_length_3755_cov_3.773243"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLE93_02335"
     CDS             <1..823
                     /locus_tag="PLE93_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNH85917.1"
                     /translation="KPIRDRVLRRLDVERQLHGAIENEELQVVYQPIISLRTGKLRAF
                     EALTRWDHPEEGILKPELFIPIAEESDLISRIDSWVMAEAVRQAGVWNEGLDPDDRIT
                     VSVNASSRQIHKSGLAAEITDLLVRCDMRPENLAIEITENVLLTGSAVIDDVLREIHE
                     LGVRLALDDFGTGYSSLSYLSEFPLDAIKIDRAFVEGLGRGDPSTSAIADAIAQIGRA
                     LSLLVVAEAVSTETQLQMVRDLGCSMAQGFLISPPLSVEQATDFVKGSGLPDLTR"
     gene            complement(803..2131)
                     /locus_tag="PLE93_02340"
     CDS             complement(803..2131)
                     /locus_tag="PLE93_02340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH85918.1"
                     /translation="MYSTGGVADRFIPLWSRIEGRLGGSLPAAIRFWDGSEIGPGEAG
                     SCPDVIVLRSKRAMTYVVTRPDQLGVARAFVSGDLVLEGSIERVMAAGARLYGFDFTM
                     QDKLEAVKLAVGLGAIKVPPPKPPESEARPKGRLHSLRRDSESIQHHYDVSNDFYRLI
                     LGPTMVYSCAYFNSADDSLEEAQTRKLDVVCQKLRLEPGERLLDIGCGWGSMAIHAAS
                     NYGVKVVGVTLSIAQAERARQRVAEAGLEDRIEIRIQDYREVSDGPFDKLCSIGMFEH
                     TGSDIDGYFAETAKLGKPGGLALHHGICRQHSDEESPNTFINHYVFPDGELHRVARVI
                     AGLERSGQELRDVENLREHYALTLRHWVANLAANQEAAIAEVGEERERIWRLYMTASF
                     LAFERGDVGVNQILGVIPEHEQVALYRPSPGLALARPSFVREEVSAGQVG"
     gene            complement(2230..2433)
                     /locus_tag="PLE93_02345"
     CDS             complement(2230..2433)
                     /locus_tag="PLE93_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNH85919.1"
                     /translation="MPTGTVKWFSDEKGFGFITPDDGSKDVFVHHSAIQADGFRTLAE
                     GAKVSYESEEGPKGPAAASVETI"
     gene            complement(2521..2976)
                     /locus_tag="PLE93_02350"
     CDS             complement(2521..2976)
                     /locus_tag="PLE93_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNH85920.1"
                     /translation="MAEPSKPLGDAVAEATEAASLPKADRPKKRTAKPQDVFGGEMRR
                     RDLLPLLIFHLIRAEPSYGNQLIDAIETLTQGVISVNPNTMYPLLRDLEGKGMIEGSW
                     EHPDKRTRRFYSITESGEAAYQELLPEIEPFLDSVIRSVTMIKAEVYGS"
     gene            3037..>3755
                     /locus_tag="PLE93_02355"
     CDS             3037..>3755
                     /locus_tag="PLE93_02355"
                     /inference="COORDINATES: protein motif:HMM:NF025843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH85921.1"
                     /translation="MDSGTGQLFESEASFNGAVEVVHVIWAAEDDGFAVVEVRDDSGE
                     EFVATGTIGHLKPGERAKLTGQWSEHQKYGPQVQVFTALPMDPSDRAGQVAYLSSLRH
                     IGPIRAEALCELHGPEVLEKIAADPEGILGSLPGLGAKQREAALESWYETRAVRDLHV
                     ELAPHGLAHLAGKIHARFGDKAMRTIREDPYSLTEIDGVGFARADTIAIAAGFPPESD
                     RRAQAAAWYLLGEAERRGHTHL"
BASE COUNT          642 a         1241 c         1208 g          664 t
ORIGIN      
        1 caaaccgatt cgcgaccggg tgctgcgtcg actggacgtc gagcgccagc ttcacggcgc
       61 gatcgaaaac gaggagctgc aggtcgtcta ccagccgatc atcagcttgc gcaccggcaa
      121 gctgcgggcc ttcgaggcgc tgacccgctg ggatcatccc gaggagggca tcctcaagcc
      181 ggagctcttc atcccgatcg ccgaggagag cgacctgatt tcccggatcg acagctgggt
      241 gatggccgag gcggtcaggc aggcgggggt ctggaacgag ggcctcgatc ccgacgaccg
      301 gatcaccgtt tcggtcaacg cttcatcgcg ccagatccac aaatccggtc tcgcggccga
      361 gatcaccgat ctgctggtca gatgtgacat gcggcccgaa aacctggcga tcgagatcac
      421 cgagaacgtc ctgctgaccg gaagtgccgt gatcgacgac gtgctgcggg aaattcacga
      481 gcttggcgtc cggctcgcgc tcgacgattt tggcaccggc tactcgtcac tcagctacct
      541 gagcgagttt ccgcttgacg cgatcaagat cgaccgggcc ttcgtcgagg gtctcggccg
      601 cggtgacccc agcacctctg cgatcgccga cgcgatcgcc cagatcggcc gggcgctttc
      661 gctgctggtc gtggcggagg cagtctcgac cgaaacccag cttcagatgg tccgggacct
      721 cggctgctcg atggcgcagg gctttctgat ctcgccgccg ctcagtgtcg agcaggcgac
      781 cgacttcgtc aaaggctccg gcctacccga cctgacccgc tgagacctct tcccgcacga
      841 aggagggccg ggccagcgcc agtcccggcg agggccggta cagggcaacc tgctcatgct
      901 ccggaatcac cccgagaatc tggttgacgc cgacgtctcc gcgttcgaag gcgaggaagc
      961 tggcggtcat gtagagccgc cagatccgtt cccgttcttc cccgacctcg gcgatcgccg
     1021 cttcctggtt ggcggcgaga ttggcaaccc agtggcgcag ggtgagcgcg taatgctcgc
     1081 gcaggttctc gacgtcccgc agttcctggc cggagcgttc gagcccggcg atcacccggg
     1141 cgacccggtg aagctcgccg tcgggaaata catagtggtt gatgaacgtg ttcggtgatt
     1201 cctcatcgct gtgctgcctg cagatcccgt ggtgaagtgc gaggccgccc ggcttgccca
     1261 gcttcgcggt ctccgcgaaa tagccatcga tgtcggaacc ggtgtgctcg aacatgccga
     1321 tcgagcaaag cttgtcgaag ggaccgtcgc tgacctcgcg gtagtcctgg atgcggatct
     1381 cgatcctgtc ctcgagcccc gcttcggcaa cccgctgcct ggcccgctcc gcctgggcga
     1441 tcgaaagggt gaccccgacc actttcactc cgtagttgga tgccgcatga atcgccatcg
     1501 atccccagcc gcagccgatg tcgagcagcc gctcgcccgg ttcgagcctg agcttctggc
     1561 agaccacgtc cagtttgcgg gtctgggcct cttcgaggct gtcgtcggcc gagttgaagt
     1621 aggcacagga gtagaccatg gtcgggccga ggatgagccg gtagaaatca ttcgagacgt
     1681 cgtagtgatg ctggatcgac tcggagtcgc gacggagcga atgcagcctt cccttcggcc
     1741 tggcctccga ttccggcggc ttcggcggcg gcaccttgat cgctcccaga ccgaccgcca
     1801 gcttcactgc ctcgagcttg tcctgcatcg tgaagtcaaa tccgtaaagc cgggctccgg
     1861 ccgccatcac ccgctcgatg ctgccttcga gcacgaggtc accggagacg aaggcccggg
     1921 ccaccccgag ctggtccggc cgggtcacca cataggtcat cgcccgcttg cttcgcagca
     1981 cgatcacgtc ggggcaggag cccgcttcgc ccggtccgat ctcgctgccg tcccagaagc
     2041 ggatcgcggc cgggagcgac ccgcccagcc tgccttcgat ccgggaccag agaggaatga
     2101 aacgatcggc gacaccacct gtgctgtaca tgcccacgaa agttactccc ggccggacct
     2161 cggctcaagg agctgcaatg aaaacggccg cccgggggcg gccgtttcga acagcttccg
     2221 agaccgggtt cagatggtct cgacggaagc ggctgcgggg cccttcgggc cctcttcgga
     2281 ctcgtagctc accttggcgc cctcggccag ggtgcgaaac ccgtcggcct ggatcgcgga
     2341 gtggtggacg aagacgtcct tggagccatc atcaggtgtg atgaatccga agcccttctc
     2401 gtcactgaac cacttgactg tcccagtcgg cataacttcc tccaacatca tgatgcggtg
     2461 ccggttctct cctcccggcc tgacgcgggg caaattacca cttgagcgcc gggcggcgct
     2521 tcaggagccg taaacctcgg ccttgatcat ggtcaccgac cggatcaccg aatccaggaa
     2581 aggctcgatc tcgggcagca gttcctggta agcggcttcg ccagattcgg tgatcgagta
     2641 gaagcggcgg gtccgcttgt cggggtgctc ccagctgcct tcgatcatcc ccttgccttc
     2701 gaggtcgcga agcagcgggt acatcgtgtt cgggttgacc gagatcacgc cctgggtcag
     2761 ggtctcgatc gcgtcgatca gctggttgcc gtaggaaggc tcggcccgga tcaggtggaa
     2821 gatcagcagc ggcaggagat cccgccggcg catctcgcca ccgaacacgt cctgtggctt
     2881 ggccgtccgc ttcttcgggc ggtctgcttt gggcaggctc gcagcctcgg tcgcttcagc
     2941 gaccgcatct ccaagcggct tggacggctc ggccatagga ggcgagtcta aacccgtccc
     3001 gccgttgtta accgggagga ccattaacct cagcgggtgg attcaggcac gggacaactc
     3061 ttcgagagcg aagcctcgtt caacggcgcg gtcgaggttg tccatgtgat ctgggcggcc
     3121 gaggatgacg gcttcgcggt ggtcgaagtt cgcgacgatt ccggtgagga attcgtcgcc
     3181 accggaacga tcggccacct gaaaccgggt gagcgggcca aattgaccgg gcaatggagc
     3241 gagcaccaga agtacgggcc ccaggtccag gtgttcacgg ccctgccgat ggatccctcc
     3301 gaccgggccg gccaggtcgc atatctcagc tcgctccgtc acatcggccc gattcgggcg
     3361 gaggctctct gtgaactcca cggccccgaa gtgctggaga agatcgccgc cgaccccgag
     3421 gggatcctcg gttccttgcc cgggctcggg gcaaagcagc gcgaggcggc gctcgagagc
     3481 tggtacgaaa cccgggcggt gcgggacctc cacgtcgagc tggcacccca cggtctcgcc
     3541 catctggccg gcaagatcca tgcccgcttc ggcgacaagg cgatgcggac catccgcgaa
     3601 gacccgtatt cgctgaccga gatcgacggg gtcggtttcg cccgggccga cacgatcgcg
     3661 attgccgctg gcttcccgcc ggaatccgac cgccgggccc aggccgccgc ctggtacctg
     3721 ctgggcgagg ccgagcgccg tggccacacc cacct
//
LOCUS       DAOQFI010000085         3754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11142_length_3754_cov_3.583401, whole genome shotgun sequence.
ACCESSION   DAOQFI010000085 DAOQFI010000000
VERSION     DAOQFI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11142_length_3754_cov_3.583401"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="PLE93_02360"
     CDS             <1..1531
                     /locus_tag="PLE93_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85922.1"
                     /translation="PPLAFIAVISAVLLAIFPKGFPNYDTIYYLLWGREIADGLSPDY
                     GAPLAPTPHPLYDLLGALFSPLGDGSITVAVVLAYVSLGLLAWLVYRLGSEWFDRWIG
                     VLAAFLVMTSAPVLSNGLRAYIDIPYMVLCLAALLIETKKPKAGWPVLVLLALAGLLR
                     PEAWLFSGFYFLWLALDFHVSPAEGADTSAPSRRAGISLGRLRIQLRESGIDRNLILM
                     ACLTAAGPVIWVLFDLITTSNPLYSFTGTRETVETLERDTGPLDVIIYGPRRLGEVMQ
                     WPGMVGAFFGVILTLWAMPKRAAIGVISAFLALVAFALMGASGLAIIPRYTMLAAAIL
                     FIFTAAAVLGWRLLEPGHRLRRIWQVAAAITVALWLIWLPNQYDLLSTVDTDLSDQAL
                     VERDLEALVDDGAFNPPSDLPGETSVEDCLPISVPNHRAVPRLAFWLDIRPSDVVSIA
                     AADEQPDSGFFLAPANEFTVENFILDPGEPGRAVTTPPPGFEEVARNRSWVLYTSCLA
                     G"
     gene            1571..1918
                     /locus_tag="PLE93_02365"
     CDS             1571..1918
                     /locus_tag="PLE93_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85923.1"
                     /translation="MSAVVLGLLFIFILIMVTRPALLVGVSADDLAHSLANTTSPSGG
                     SNCRKEADGNWTCNFAPPDGSWRRYEVSVDWLGCWDATLPGADTSTSGTSSSPYDKSG
                     CIELGDVIRIESD"
     gene            complement(1922..2203)
                     /locus_tag="PLE93_02370"
     CDS             complement(1922..2203)
                     /locus_tag="PLE93_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85924.1"
                     /translation="MKLYVCWGTFQVPGAREHPCKIAYDALVQADFEPEVIKAHSVGF
                     LPRAVQTGARKKVEDATGSKWVPALETDEGEWISGSREIVAWAEQHARV"
     gene            complement(2280..>3754)
                     /locus_tag="PLE93_02375"
     CDS             complement(2280..>3754)
                     /locus_tag="PLE93_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNH85925.1"
                     /translation="SSFDNPITGGPALIPGEWAYVAGTWANGVGRLYVNGALVASGSM
                     PHPPSGISTFYLGYGDQAPWLHGDLDEVAYYDHALPAGRIGMRNAVGRYIETPSAPGG
                     PDTTRPDVEIKYPQKNALYVRTKAPKAAFTCSDPDGDVDIASCEADVDGTPINFGAPL
                     PDSPGAHYLRVVAKSQSGLTTVDIHDYKITSFDQVVLKDQPEAYFRFDESSGSGVLVD
                     QTASHNGAFKNDNAPGGTGISGDGSATRAFFGKGGYAFVNGIPASQTGSTLEAWVKPD
                     DAGDMAILEHGGAGALFIRGGKVVFRHVGSEIESPLGAAPVGQTTQVVGVWDGVNMIL
                     YVNGVEVASKEAPGQPSGSSTLYLGLANVGLASPWLRGSLDEVSVYSHALEAGQVDQH
                     FRADPPPPAEDNPSSHDPDQPAMDDHVVDDPDQPVGDDPDQSSTDDPVGSVEAESGGS
                     SPKSGVKSNSKARRSALKKCQRMSNRSKRNRCLRHVRNRF"
BASE COUNT          627 a         1220 c         1149 g          758 t
ORIGIN      
        1 gccgccgctt gccttcatcg ccgtcatctc ggcggtcctg ctggcgatct ttcccaaggg
       61 cttcccgaac tacgacacga tctattacct gctctggggc cgggagatcg ccgatggcct
      121 cagcccggat tacggcgccc cgctcgcccc aaccccgcac ccgctctacg accttctcgg
      181 ggcgcttttc tcaccgctgg gcgacggctc gatcaccgtc gcggtcgtcc tcgcctacgt
      241 aagcctcggc ctgctggcct ggctggtcta ccggctgggc tcggaatggt tcgatcgctg
      301 gatcggggtc ctcgccgcgt ttctggtgat gacgagcgca ccggtcctga gcaatggtct
      361 ccgggcatac atcgacatcc cgtacatggt gctctgcctt gccgccctgc tgatcgaaac
      421 gaagaagccg aaggctggct ggcccgtact cgtgttgctt gccctggccg gcctgctcag
      481 accggaggcc tggctcttct ccggcttcta cttcctctgg ctcgccctcg atttccacgt
      541 gtcgcctgcg gagggcgccg acacctcagc tccgtctcga cgcgccggga tcagcctcgg
      601 acgcctgaga atccagctga gggagtcagg gattgacagg aacctgatcc tgatggcctg
      661 cctgacggcg gccggaccgg tgatctgggt tctcttcgac ctgatcacaa cctcgaaccc
      721 cctctactcc ttcaccggca cccgcgagac ggtcgagacg ctggagcgag acaccggacc
      781 actcgacgtg atcatctacg gaccacgccg gcttggcgaa gtgatgcagt ggccgggcat
      841 ggtaggtgcc ttcttcggag taatcctgac cctttgggcg atgccgaagc gggccgctat
      901 cggggtgatc tcggctttcc tggccctggt cgccttcgcc ctgatgggtg cttcgggcct
      961 ggcgatcatc ccccgctaca cgatgctggc cgcggcgatc ctgttcatat tcacggctgc
     1021 cgcggtacta ggctggcggc tgctcgagcc cggtcacagg ctgcggcgaa tctggcaggt
     1081 ggcagccgca atcacggtcg cgctctggct gatctggctg cccaaccagt acgacctgct
     1141 cagcaccgtc gacaccgacc tgagcgatca ggccctggtc gaaagggacc tcgaggccct
     1201 tgtcgatgac ggtgccttca atcccccgag cgacctccct ggggagacct cggttgagga
     1261 ctgcctgccg atctcggtcc cgaaccatcg ggccgtgccc cgtctcgcct tctggctgga
     1321 cattcggcca tctgatgtcg tgtcgatcgc cgccgcggac gaacaaccag attccggctt
     1381 cttcctcgcc ccggccaacg agttcacggt cgaaaacttc atcctcgacc cgggcgagcc
     1441 gggccgtgcc gtcaccacgc ctccccccgg tttcgaggag gtcgcccgca accggtcctg
     1501 ggtgctctac accagctgcc tggcgggctg agccaccgtg tggtggaagg gcgtaaagat
     1561 cctcggggcc ttgagcgccg tcgtcctggg gctcctgttc atcttcattc tgatcatggt
     1621 cactcggcct gcacttctgg tcggcgtttc agctgatgac ctggcccact cgctggccaa
     1681 caccacctcg ccgagcggcg gcagtaattg ccgcaaggaa gctgatggaa actggacctg
     1741 caacttcgcc cctccggatg gatcctggag acgttacgag gtgagcgtcg actggctggg
     1801 ctgctgggac gccaccttgc cgggcgccga cacaagcacc tccggaacct ccagctcccc
     1861 ttacgacaag tccggctgca tcgaactggg cgacgtgatc aggatcgaga gcgactgaag
     1921 gtcagacccg ggcgtgctgc tcggcccagg cgactatctc tctggatccc gagatccatt
     1981 ctccctcgtc tgtttccaac gccggcaccc acttgcttcc cgtggcgtcc tctacctttt
     2041 tccgggcgcc ggtctgaacg gcgcggggca ggaaaccgac gctgtgggcc ttgatgacct
     2101 ccggctcgaa gtcagcctga accagtgcgt cataggcgat cttgcaggga tgctcccgcg
     2161 ctcccggcac ctgaaaagtt ccccagcaga cgtagagttt catgggacca gactaatcgg
     2221 cgccgtgaag ctgtcgtttt cccacatcat ttgtggaaaa agtgcggttt agcgtggctc
     2281 tagaaccggt tacgaacgtg gcgcaggcag cggttgcgct tcgagcggtt gctcattcgt
     2341 tggcacttct tcaatgcgga ccttctggcc ttggagttcg acttgacgcc ggacttcggt
     2401 gacgatccgc cgctctcggc ttccacgcta ccgaccgggt catctgtgga cgactggtca
     2461 gggtcgtcgc cgaccggttg gtcggggtcg tccactacgt ggtcatccat ggcaggttgg
     2521 tcggggtcgt ggctcgacgg gttgtcctcc gctgggggtg gaggatctgc cctgaagtgc
     2581 tggtcgacct gaccagcttc aagggcatgg ctgtaaacag agacctcgtc gagtgagccg
     2641 cgcagccacg gtgaggcgag cccgacgttg gccaatccca gatagagagt cgaggagccg
     2701 gaaggctgac cgggggcttc cttagaggcg acttccaccc cgttgacgta aaggatcatg
     2761 ttgacgccgt cccagactcc gaccacctga gtcgtttgcc cgactggtgc cgcaccgaga
     2821 ggcgactcga tctcgcttcc tacgtgacgg aaaaccacct tgccgcctcg gataaacagt
     2881 gccccggcgc cgccatgttc gagaatcgcc atgtccccgg catcgtccgg cttgacccag
     2941 gcctcgagtg tggagccggt ctgggaagcc ggtattccat tgacaaacgc gtacccgccc
     3001 ttcccgaaaa aggcgcgtgt tgcactgcca tcacccgaga tgccagttcc gccgggagcg
     3061 ttgtcgttct tgaaggcgcc gttgtgactt gcagtctgat cgaccaggac cccggagcca
     3121 gacgattcgt cgaagcggaa ataggcttcc ggttgatcct tgaggacaac ctgatcgaag
     3181 ctggtgatct tgtagtcgtg gatgtcgacc gtggtaagtc cgctctggct cttcgctacg
     3241 acccgcaggt aatgcgcgcc tggtgagtcg ggaagaggag cgccaaaatt gatcggagtt
     3301 ccgtccacgt ccgcttcaca tgaagcaatg tcaacgtcac cgtcagggtc ggagcacgtg
     3361 aaggctgcct tcggtgcctt ggtcctgaca tacagcgcgt tcttttgcgg gtacttgatc
     3421 tccacgtcag gccgggtggt atccggaccg ccaggagccg agggagtttc gatgtacctg
     3481 ccgaccgcgt ttcgcatgcc gatccggcca gccggcaagg cgtggtcgta ataggcgacc
     3541 tcatcgagat ccccgtggag ccagggagcc tggtcgccgt aaccgagata gaaagtagaa
     3601 atgccagaag gtgggtgagg catggaaccg gaagcaacca gcgcgccgtt cacataaagc
     3661 cttcccactc cgttcgccca ggtaccggcc acataggccc attcgccggg gatcaaggca
     3721 gggcctccgg tgatcggatt gtcgaaactg ctcc
//
LOCUS       DAOQFI010000086         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11227_length_3740_cov_3.092266, whole genome shotgun sequence.
ACCESSION   DAOQFI010000086 DAOQFI010000000
VERSION     DAOQFI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11227_length_3740_cov_3.092266"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLE93_02380"
     CDS             <1..759
                     /locus_tag="PLE93_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNH85926.1"
                     /translation="DRSAMDGFLSRVEKLDTPGDMSAAQDSLTLVYQLRASAMDDIAD
                     QMSTALGDEGAERARRQIAAQMEVLSAADVLYNRVTRHQIDNTIANNGASAPAMPVST
                     FIEDPQDWITVDAINGALDDVSGSTSTTAPDDGLTHGTGIDPASSVTVDGTTLSPDTT
                     TTIAASTDPTVEVTVMNQGGATETDVTVGVSVDGGTSLEAKIPEIAAGSTGTATIPLT
                     PAPTGEVTLDVSVDAVPGEQFTTNNEASYTVSFG"
     gene            767..1615
                     /gene="pheA"
                     /locus_tag="PLE93_02385"
     CDS             767..1615
                     /gene="pheA"
                     /locus_tag="PLE93_02385"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNH85927.1"
                     /translation="MRVAYLGPAGTFSEDALEVSRFAPEGFEAVVCETIPAAIEAVAA
                     GDADRALVPVENSIEGSVRPTLDGLIEHADAVRIAGEFDHEIRSALIARTALDPGSIE
                     VVISHPQPLAQCALFLRTELGQAEQRVATSTSAAVREVAESTGPWAALAPARAARIYE
                     CEVIREGIEDEPGNVTRFLWLAPESAENPVVGDQGGQWKTSLAFAELGADHPGALVEA
                     LHEFSDRRVNLVRIESRPMRRELGRYRFFIDIEGREDADDVTAAIAGLRSRAEWVRVL
                     GSYPAN"
     gene            complement(1646..>3740)
                     /locus_tag="PLE93_02390"
     CDS             complement(1646..>3740)
                     /locus_tag="PLE93_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNH85928.1"
                     /translation="DSDTVDFGPNYDGSQREPLVLPSRFPNLLVNGSSGIAVGMATNI
                     PPHNLTEVAGAVAAYIDNREIDLEGLMKHIKGPDFPGGGLMDPGGIRDAYQTGRGSVK
                     VRARIHSEPLKGGKDALIVSELPFTVKKGGDSGVIAKIAQLVRDKKLDGISDLRDETD
                     RTGMRLVIELKRGAVPNTVLANLFKKTQLQTSFGMNMVSLVDGVPRTLGIRDMIVHYV
                     EHQREVVTRRTQYKLRRAEARAHILEGLLIALDNLDAVIALIRGSSDPDVARDGLISE
                     FDLSREQAQAILDMRLQRLTALESDKIREEHAELMELIGELRAILGDEDRVYGLVRDE
                     LLELVETHGDERRTEFQHFGGDIDIEDTIAEQQMVISLTNTGYVKRLPVDSYRQQRRG
                     GVGVTGMNLKEGDYIEHLHICSTHDFLLFFTNFGKVYRKKVYELPEGSRQARGSALVN
                     LLPLREGERVMAVIPTREFKENKYLVFATAQGQVKKTEFLDYNTPIKADGIIAIKIRE
                     GDELVGVQLTSGEDDLLMVSKSGHASRFHEDQARPMGRGTAGVRGMNVSQKDNRVLSL
                     TVARDDSDLLVVTENGYGKRTGISEYPVKNRGTKGVLTIKLTEAKGGLAGALIVREHQ
                     ELMFITENGMVQRTGAGGISKTGRATQGVKVMNVKDGDRISAVALVVEAANGENGDPE
                     GEEAPEQPELEATEE"
BASE COUNT          597 a         1258 c         1215 g          670 t
ORIGIN      
        1 gaccgctcgg cgatggacgg cttcctcagc cgggtcgaga agctcgacac gcccggggac
       61 atgagcgccg cccaggattc cctcaccctc gtctaccagc ttcgcgcctc ggcgatggat
      121 gacatcgccg atcagatgag caccgcactc ggggacgaag gcgccgagcg ggcccggcgc
      181 cagatcgccg cccagatgga agtgctgagc gccgccgacg tcctctacaa ccgggttacc
      241 cgccaccaga tcgacaacac gatcgccaac aacggcgcca gcgccccggc gatgccggtc
      301 agtaccttca tcgaggatcc ccaggactgg atcacggttg acgcaatcaa cggcgcgctc
      361 gacgacgttt ccggctccac cagtacgacg gcacccgatg acgggctgac ccacggcacc
      421 ggaatcgacc cggcctcgtc ggtcacggtg gatggcacca cgctcagccc ggacaccacg
      481 acgacgatcg cggccagcac cgacccgacc gtggaagtca cggtcatgaa ccagggcgga
      541 gcgaccgaga ccgatgtgac ggtcggcgtc tccgtggacg gcggcaccag cctggaagcc
      601 aagatccccg agatcgcagc cggttcgacc ggcaccgcga cgattccgct gactcccgct
      661 ccgaccggtg aagtgaccct ggacgtgtcg gttgacgccg tccccggtga gcagttcacc
      721 accaacaacg aagcttccta caccgtcagc ttcgggtagg cacgccatgc gtgtcgcgta
      781 tctcggcccc gccggcacct tcagcgagga tgcgctcgag gtcagccgct tcgcgcccga
      841 gggcttcgag gcggtggttt gcgaaacgat tccggccgcg atcgaggcgg tcgcggccgg
      901 ggatgccgac cgggccctgg ttccggtcga aaactcgatc gaggggtcgg tccgccccac
      961 tctcgacggc ttgatcgagc acgccgatgc ggttcgcatc gccggcgagt tcgaccatga
     1021 gattcgttcg gccctgatcg cccggacggc ccttgacccc ggatcgatcg aggtggtgat
     1081 ctcccacccc cagccactgg cccagtgcgc gcttttcctc agaacggaac tcggccaggc
     1141 cgagcaacgg gtcgcgacca gcaccagtgc cgcggttcgc gaggtcgcgg aatcgaccgg
     1201 accgtgggcc gcccttgctc ccgcccgcgc cgcccggatt tacgaatgcg aggtaatccg
     1261 ggagggcatc gaggatgaac cggggaatgt gaccagattc ctctggctgg cccccgaatc
     1321 tgccgagaac ccggtggtcg gggatcaggg cggccagtgg aagaccagcc tcgccttcgc
     1381 tgaactgggt gccgatcacc cgggtgcgct ggttgaagcc ctccacgaat tttccgaccg
     1441 ccgcgtgaac ctggtccgga tcgagtcccg cccgatgcgc cgcgaactcg ggcgctaccg
     1501 cttcttcatc gacatcgaag gccgcgagga cgccgacgat gtcaccgccg caatcgccgg
     1561 actccggtca agagccgagt gggtaagagt cctcggctcc taccccgcaa actgactgca
     1621 agtcgccatg ttgagtgacc cggggctact cctcagtggc ttcgagttcg ggttgctcgg
     1681 gggcttcttc gccttccggg tccccgttct cgccgttggc ggcttcgacc accagggcga
     1741 cggcgctgat ccggtcgccg tccttgacgt tcatgacctt gacgccctgg gtggcgcgac
     1801 cggtcttgct gatgccgccc gccccggtcc gctggaccat tccgttctcg gtgatgaaca
     1861 tcagctcctg atgctcacgc acgatcaggg cgccggccag gccacccttg gcctcggtca
     1921 gcttgatcgt caggacgccc ttggtacccc ggttcttgac cgggtactcg gagatgccgg
     1981 ttcgcttgcc gtaaccgttt tcggtcacga cgaggaggtc cgaatcatcc cgggccacgg
     2041 tcagtgacag cacccggttg tccttctggc ttacgttcat gccgcgcacg ccggcggtgc
     2101 cacggcccat cggccgggcc tgatcctcgt ggaagcgcga agcgtggccg gacttcgaga
     2161 ccatcaggag gtcatcctcg ccggaggtca gctgaacccc gaccagctcg tcgccctcgc
     2221 ggatcttgat cgcgatgatt ccgtcggcct tgatcggcgt gttgtagtcg aggaactcgg
     2281 tcttcttgac ctggccctgc gcggtcgcga agacgaggta cttgttctcc ttgaactccc
     2341 tggtcgggat gaccgccatc acgcgttcgc cttcccggag gggaagcagg ttgaccaggg
     2401 ccgagccgcg ggcctggcgc gagccctcgg ggagctcgta gaccttcttg cggtagacct
     2461 tgccgaagtt ggtgaagaac agcaggaagt catgggtcga gcagatgtgg agatgctcga
     2521 tgtagtcgcc ttccttcagg ttcattccgg tgaccccgac cccgccgcgg cgctgctggc
     2581 ggtaggagtc gaccggcagg cgcttgacgt agccggtgtt ggtcagcgag atcaccatct
     2641 gctgctcggc gatcgtgtct tcgatgtcga tgtccccgcc gaagtgctgg aactcggtcc
     2701 ggcgctcgtc gccatgggtc tcgaccagtt cgagcagctc gtcccggacc aggccgtaaa
     2761 cccggtcctc gtcaccgagg atcgcccgga gctcgccgat cagttccatc agctcggcat
     2821 gttcctcgcg gatcttgtcc gactcgagcg cggtcagccg ctgcagccgc atgtcgagaa
     2881 tggcctgggc ctgttcgcgg ctcaggtcga actcgctgat cagtccgtca cgggcaacat
     2941 ccggatcgga cgagccgcgg atcagggcga tcaccgcgtc gaggttgtcg agcgcgatca
     3001 gcaggccttc cagaatgtgg gcccgggctt cggcgcggcg cagtttgtac tgggtccgcc
     3061 tggtcactac ctcgcgctgg tgttcgacgt agtggacgat catgtccctg atgccgaggg
     3121 ttcgcggcac cccgtcaacc agcgagacca tgttcattcc gaacgaggtc tggagctggg
     3181 tcttcttgaa gaggttggcg agcaccgtgt tcgggaccgc tccgcgcttg agctcgatca
     3241 ccaggcgcat gccggtccgg tcggtctcgt cccgcagatc ggagatcccg tccagcttct
     3301 tgtcccggac cagctgggcg atcttggcga tcacgccgct gtcgccgccc ttcttcacgg
     3361 tgaacggaag ttcggagacg atcagggcgt ccttgccgcc cttgagcggt tcggagtgga
     3421 tcctcgcgcg gaccttgacc gagccgcggc cggtctggta ggcgtcgcgg attccacccg
     3481 ggtccatcag cccgccgccg gggaagtcgg ggcccttgat gtgcttcatc aggccttcga
     3541 gatcgatctc ccggttgtcg atgtaggcgg cgaccgcgcc ggcaacctcg gtcaggttgt
     3601 gcggcgggat gttggtcgcc atgccgaccg cgatccccga ggaaccgttg accagcaggt
     3661 tcgggaaacg ggacggcagg acgagcggct cccgctggga accgtcgtag ttcgggccga
     3721 aatcgaccgt gtccgagtcg
//
LOCUS       DAOQFI010000087         3718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11349_length_3718_cov_3.643462, whole genome shotgun sequence.
ACCESSION   DAOQFI010000087 DAOQFI010000000
VERSION     DAOQFI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11349_length_3718_cov_3.643462"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1681
                     /locus_tag="PLE93_02395"
     CDS             131..1681
                     /locus_tag="PLE93_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH85929.1"
                     /translation="MTVRRAGCWIDGREVPARSGETLETMNPATGEQVGSVPVDSAGD
                     VAAAVARIRGNQPAWEALGFKGRRRWLNELRDWLLDNSDRVADTLKSETGKVRAEATI
                     ETPYLADQINFYGSKAEKFLGEQKVRPNTMLAATRKLRLQYRPYQVVGLISPWNFPLV
                     LSLGDAIPALMAGAAVVMKPSEITPLGLNEIVDAWRNEIGAPDVLHVVNGGAETATAL
                     VDHSDFVGFTGSDVTGKKVMARAAETLTPVSLELGGKDPMVVLEGGNMDRAVNGATWG
                     SMLNSGQMCISVERIYVEDGAYDEFVEKLTDSVGELKQGIDGETAACDVGAMTMLSQL
                     EKVEKQVDDAVEAGARVLTGGKRGEGPGQWFEPTVLVDCTPDMSVMEDETFGPVVTVT
                     RVSDEEEAIRLANDSRYGLCASVFGPRGRAEKVARRIEAGAVNVNDIVFNMLAPGLPM
                     GGWKESGIGYRNGEYGIRKYCRSEAIVSARTLLRSEPLWYPYTPERHALVSKVTKFIG
                     GRGLRRFR"
     gene            complement(1901..2302)
                     /locus_tag="PLE93_02400"
     CDS             complement(1901..2302)
                     /locus_tag="PLE93_02400"
                     /inference="COORDINATES: protein motif:HMM:NF021440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HNH85930.1"
                     /translation="MAMKVTAKARSREDRAGDPLDGLVNLFDIGIILSVGFLLAALSS
                     LNLTTEALSQNDTSPANTVPENSVVTQPDETTEQITIEPGQTVVGQGEPLGTVYQLDD
                     GRTILVKPSTGTTGATGATGTTGVTGSTLNP"
     gene            complement(2302..2991)
                     /locus_tag="PLE93_02405"
     CDS             complement(2302..2991)
                     /locus_tag="PLE93_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNH85931.1"
                     /translation="MQIENFLFSVSDALRWPVLVAVLLALAWSIVEIGILLAEIWRRR
                     WRSINALENAVDQAEVDIVQGDQIGAISALSSVSWSRPMRETMTSIVTLRPSPDAENR
                     IAKRLADYDYNSLRRLERTRILVRMGPALGLMGTLIPLAPALAGLADGNVTELTDNLR
                     VAFGVTVAGLLTGALSFGVSLIRDRIYAQDFSDVEFAAANLAPDKHLAGHFVPSPVEH
                     GHSTQVSMGNP"
     gene            3101..>3718
                     /locus_tag="PLE93_02410"
     CDS             3101..>3718
                     /locus_tag="PLE93_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85932.1"
                     /translation="MLRPLKTSLGLIAFACVLILPNFAQAFDAGVRTPLLTDRSPGAV
                     SVPFEIPVRQSAAVPQPLDFEIAVEGFRLDPAPAADLRIGSLDMVTDAGDFDNKAIYS
                     NGPAIDGARTWTLDWQLSNHPITATVEDGTGLGPAGEKTVDDGSTLISFTVPGNYHGF
                     RLMGLSLRFNQQEKGIPTPGIGAVNPTAPGSYLIREFARHLSSVVA"
BASE COUNT          638 a         1245 c         1237 g          598 t
ORIGIN      
        1 cggccgcagt cggcatccca ccggaggccg acatcgacgt cacctacgcg ggcgatcggg
       61 ccacggtcat ccgcgtccgc ccctgagccc ggccggttat ctcccctcgc accacgccac
      121 gaggtgagcg atgacggtgc gcagggcagg ctgctggatc gacggccgcg aggtccccgc
      181 gaggagcggc gagaccctgg agaccatgaa ccccgccacg ggcgagcagg tcgggtcggt
      241 cccggtcgac tcggccggcg acgtcgccgc ggcggtcgcc cgcatccgcg gcaaccagcc
      301 tgcctgggag gcgcttggct tcaagggccg ccgccgctgg ctgaacgaac ttcgcgactg
      361 gctgctcgac aattccgacc gggtcgccga cacgctcaag tccgaaaccg gaaaggtgcg
      421 ggccgaagcg acgatcgaga ccccctacct ggccgaccag atcaacttct acgggtccaa
      481 ggccgagaag ttcctcggcg agcagaaggt caggccgaac acgatgctgg ccgcgacccg
      541 caagctcagg ctccagtacc gcccttacca ggtggtcggt ctgatcagcc cctggaactt
      601 cccgctcgtg ctctcgctcg gggacgcgat cccggcgctg atggccggcg ctgccgtggt
      661 catgaagcct tccgagatca ctccgctcgg cctgaacgag atcgtcgacg cctggcgcaa
      721 cgagatcggc gcccccgacg tgcttcacgt cgtcaatggt ggcgccgaaa ccgcgaccgc
      781 cctggtcgac cactccgact ttgtcggctt caccggctcc gacgtgaccg gcaagaaggt
      841 gatggcccgc gcggccgaga ccctgactcc ggtcagcctc gaactcggcg gcaaggaccc
      901 gatggttgtt ctcgaaggcg gcaacatgga ccgcgccgtc aacggtgcga cctggggcag
      961 catgctcaac tcgggccaga tgtgcatctc ggtcgagcgg atctacgtcg aggacggcgc
     1021 ttacgacgag ttcgtcgaga agctgaccga cagcgtcggc gagctgaagc aggggatcga
     1081 cggcgagacc gccgcctgcg acgtcggcgc gatgaccatg ttgtcccagc ttgagaaggt
     1141 cgagaagcag gttgacgacg cggtcgaggc cggagcccgg gtactgaccg gcggcaagcg
     1201 cggcgaaggt ccgggtcagt ggttcgagcc gaccgtgctg gtcgactgca cccccgatat
     1261 gagcgtgatg gaggacgaga ctttcggtcc cgtcgtcacc gtgacccggg tgagcgacga
     1321 ggaagaggcg attcgcctcg ccaacgacag ccgctacgga ctctgcgcct cggtcttcgg
     1381 cccccgcggc cgggccgaga aggtggcccg gcggatcgaa gccggcgcgg tcaacgtcaa
     1441 cgacatcgtc ttcaacatgc tggcgccggg cctgccgatg ggcggctgga aggagtcggg
     1501 gatcggctac cgcaacggcg agtacgggat ccgcaagtac tgccgttccg aggcgatcgt
     1561 ttccgcccgc accctgctcc gctcggaacc gctctggtac ccgtacaccc cggagcgcca
     1621 cgccctggtc agcaaagtca ccaagttcat cggcggccgc ggccttcgcc gcttcagata
     1681 gtccctccga aaccccgctg gtaacgccgc cccggcggct acgccgaccc ggttccgggg
     1741 atatcgagat acgacttatt gccgcatagc gcactcaact ccgatctcga cctgatgggt
     1801 cgaatcgaaa tcggagttgg gagcgttata tgcgcaaaac tcccttctcg atatccccga
     1861 aaccccgttc aggctcgact tctcgaaaac ggtttgccgg ctaggggttg agcgtggagc
     1921 cggttactcc ggtggttccg gtcgcgccgg tcgcgccggt cgtgccggtt gagggcttga
     1981 cgaggatcgt tctgccgtcg tcgagctggt agacggtgcc gagcggctcg ccctggccga
     2041 ccacggtctg gccgggctcg atcgtgatct gttcggtggt ctcgtccggt tgggtaacga
     2101 ccgagttctc ggggacggtg ttcgccggtg aggtgtcgtt ctgcgagagg gcctcggtgg
     2161 tcaggttgag cgaggaaagc gcggccagga ggaaaccgac cgagaggatg atcccgatgt
     2221 cgaagaggtt gaccaggccg tcgagcggat caccggcgcg gtcctcgcgg ctcctcgcct
     2281 tggccgtcac cttcatggcc atcagggatt ccccatcgag acctgggtgc tgtgcccgtg
     2341 ctcgaccggt gacggcacga aatgcccggc caggtgcttg tccggcgcga ggttggcggc
     2401 ggcgaactcg acatccgaaa agtcctgggc gtagatccgg tcgcggatca gcgagacgcc
     2461 gaaggagagc gcaccggtga gcaggcccgc cacggtcaca ccgaacgcga cccgcaggtt
     2521 gtcggtcaac tcggtcacgt tgccgtccgc cagtccggcc agagccggag ccagcgggat
     2581 cagggtgccc atcaggccga gtgcggggcc catccggacc aggatcctcg tccgttcgag
     2641 ccggcgcagt gagttgtagt cgtaatcggc gagtcgcttg gcgatccggt tctccgcgtc
     2701 gggggaggga cggagggtga cgatcgaggt catcgtctcg cgcatcggcc ggctccacga
     2761 aaccgaggaa agtgccgaaa tcgccccgat ctgatcgccc tgcacgatgt cgacttcggc
     2821 ctggtcgacc gcattctcga gcgcgttgat cgaccgccag cgccggcgcc agatttcggc
     2881 caggaggatc ccgatttcga cgatcgacca ggccagggcc agcagcaccg cgaccaggac
     2941 tggccagcgg agagcgtcgg aaacggagaa gaggaagttt tcgatctgca tgcttgaggc
     3001 gcccggggct tagggcagcc gaagggtcaa gatagcggag caacgctgtc aggccgcgca
     3061 actgtccggc cccgaatgtg tctatacgct gacccgcccg atgcttcgcc ccctgaagac
     3121 aagcctggga ctgatcgcct tcgcctgcgt tctgatcctg ccgaacttcg cccaggcctt
     3181 cgacgccggg gtgcgtacgc ccctgctcac cgatcgatct cccggcgcgg tcagcgtgcc
     3241 tttcgaaatc ccggtcaggc agtcggccgc ggttccccag cccctggatt tcgagatcgc
     3301 ggtcgagggt ttccgcctcg atccggcccc cgccgccgat ctccgcatcg gctcgctgga
     3361 catggtcacc gacgccggtg acttcgacaa caaggcgatc tactcgaacg gaccggcgat
     3421 cgacggggcc cggacctgga ccctcgactg gcagctcagc aaccatccga tcaccgccac
     3481 ggtcgaggac gggaccggcc tgggcccggc cggtgaaaag accgtcgacg atggctcgac
     3541 cctgatcagc ttcacggttc ccggcaacta ccacggtttc cgcctgatgg gcctcagcct
     3601 gcgattcaac cagcaggaaa aaggcatccc caccccgggt atcggtgctg tcaatccgac
     3661 cgcacccggc agctacctga tccgcgagtt tgcgcgccac ttgagcagcg tcgtggcc
//
LOCUS       DAOQFI010000088         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11395_length_3710_cov_3.491382, whole genome shotgun sequence.
ACCESSION   DAOQFI010000088 DAOQFI010000000
VERSION     DAOQFI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11395_length_3710_cov_3.491382"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLE93_02415"
     CDS             <1..473
                     /locus_tag="PLE93_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85933.1"
                     /translation="IDLLAGTSAISEIKGVEVGVVGSRGFVGGFAPHHLPDFGEPSLR
                     AVYEETSREVSALEAGLSEVATCQIRLVVLHYSPLRATVEGEPPEIFPFLGSDRMARP
                     LLEHGPDLVVHGHAHEGTHEGSIGEVPVRNVSLPVLGGEIWITELNAPVHGPIP"
     gene            complement(455..1081)
                     /locus_tag="PLE93_02420"
     CDS             complement(455..1081)
                     /locus_tag="PLE93_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNH85934.1"
                     /translation="MKESDPDKTEILTTSLKSAAAIMEHESIPYLLGGGLGTWAHGGP
                     PSSNDIDLMVRPADAERAIQAFADAGLRTERPPEQWLVKAWDGDVLIDLIFEPAGMRI
                     TEQVIERAEELNVGGMVVPVMCIDDILTTKLLAMDEHAADFLTLIQIARALREQIDWP
                     SLVERTSSSPFARAFFVLVDGLGIQPLALPGPGGVEWQIGPGRQGIGP"
     gene            1310..2212
                     /locus_tag="PLE93_02425"
     CDS             1310..2212
                     /locus_tag="PLE93_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85935.1"
                     /translation="MKSGITVGLAALVACAGLVSSAHAAFPGKNGRIIFERKASQFAG
                     NSDPWVFSGGKARRLVRISNNAYNFSYSPNGKKIVFEATVPSEELVVMNANGTRPRVI
                     TAKVHKCIGKRHPTWSPNGKEIAFVCLNRNSLSDHDVWSVNANGTGIRRISRTHDAYS
                     PAWSPRGNKIAYTTYGGSIYTVPARGGKSKLLIAEAPGGVFGGTFQGVDWSPNGRLLV
                     SDSSGDGVYTINARTGATSGDLAEIGAEPVFSPDGKKILYVGIAESRGSTRIGLWKMN
                     LDGSNKQQVTRGGYDRAPSWAPAR"
     gene            complement(2265..2337)
                     /locus_tag="PLE93_02430"
     tRNA            complement(2265..2337)
                     /locus_tag="PLE93_02430"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2302..2304),aa:His,seq:gtg)
     gene            2417..3073
                     /locus_tag="PLE93_02435"
     CDS             2417..3073
                     /locus_tag="PLE93_02435"
                     /inference="COORDINATES: protein motif:HMM:NF025510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HNH85936.1"
                     /translation="MSVRREMRGFLVGSLLFALGATPGYLGLVGVTADNLTYFLGSIF
                     FTIAGFTQLRLTGRWQRGGWSDRDAWADWWAAAIQFLGTLAFNVSTLAALLGTIDPGT
                     FERLGWRPDFIGSICFLVASVLAVEATTNRESLWDPEARNWWNTWLNMAGSVAFMISA
                     IFAWINPSTGEPLNTDWANLGTFFGAIGFFAAAALLKPQADADSNPSMNGTGPAENGD
                     "
     gene            complement(3070..>3710)
                     /locus_tag="PLE93_02440"
     CDS             complement(3070..>3710)
                     /locus_tag="PLE93_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH85937.1"
                     /translation="VLAAMNLSFYEAIDRIPLGIAVTFEFIGPLGVALITSHRRRDLI
                     WVAMAAAGILLLSGGIGEEGIELFGVVLAVVAGVFWGCYILLGKRVGEEFYGGQGLAI
                     AMVFSTVICTPFGVADGGADLLLPSVLAVGFAVAILSTTLPLSLEMEALRRLPSNVFG
                     VMMSLEPAMAATIGFLLLGQDMSAAQIAAIALVVSASAGALSSARAPAPIEP"
BASE COUNT          653 a         1257 c         1205 g          595 t
ORIGIN      
        1 gcatcgatct gctcgccgga acttcagcga tcagcgagat caagggcgtc gaggtcgggg
       61 ttgtcggcag ccgcggcttc gtcggcgggt tcgcgccgca tcacctgcct gacttcggcg
      121 aaccctcgct acgcgccgtc tacgaggaga cctcccgcga ggtttccgcc ctggaggccg
      181 gtctgagtga agtggcgacc tgccagatcc ggctcgtcgt ccttcactat tccccgcttc
      241 gggcgacggt cgaaggcgag cctccggaga tcttcccgtt cctcggcagc gaccggatgg
      301 cgaggcccct gcttgagcac gggccggatc tggtcgttca cggccatgcc cacgagggaa
      361 cacacgaagg cagcatcggc gaggtcccgg tgcgcaacgt gtcgctgccg gtgctcgggg
      421 gcgagatctg gatcaccgaa ctgaacgccc ccgttcacgg cccgatcccc tgacggcccg
      481 ggccgatctg ccattcgaca ccgccggggc cgggcagggc gagcggctga atgccgagcc
      541 cgtcgaccag cacgaagaag gcccgcgcga aaggactcga ggaagtccgt tcaaccagag
      601 acggccagtc gatctgctcg cggagcgccc gggcaatctg gatcagcgtg aggaagtcgg
      661 cggcatgctc gtccattgcc agcaacttgg tcgtgaggat gtcgtcgatg cacatcaccg
      721 gcaccaccat gccgcccacg ttcagttctt cagcccgctc gatcacctgc tcggtaatcc
      781 gcatgccggc cggttcaaag atcagatcga tcaggacgtc gccgtcccag gccttgacca
      841 gccattgctc gggcggcctt tcggtgcgaa ggccggcgtc ggcgaatgcc tggatcgccc
      901 gttccgcgtc ggcgggcctg accatcaggt cgatgtcgtt gctggaaggc ggccccccat
      961 gcgcccaggt cccgaggccc cctccgagga ggtagggaat gctttcgtgc tccattatcg
     1021 ctgccgccga cttcaggctg gtggtcagga tctcggtctt gtcggggtcg ctctctttca
     1081 tgccgcaacc cgccgggtac ccccgtcaag cgaggagaaa acggtccggt gcgccgcctt
     1141 cccgggcgga ctttgccgca gcccgaatcg ctagaatcgg cggcgaattg atggatcggg
     1201 ttgggggctc gagttaattg atcaccggcc ggctttggcg gaccagtcgc ggggaaagcc
     1261 ctgcgtggaa ctctgcccgg gttcggcctg gagaagggaa aacagaagga tgaagtcagg
     1321 aatcaccgta ggtctggcgg cgctggtcgc gtgcgccggg ctcgtctcgt cagcgcatgc
     1381 tgcctttccg ggcaagaacg gcaggatcat cttcgaacgc aaggccagcc agttcgcggg
     1441 caactcggat ccctgggtgt tctcgggtgg caaggcgagg cggctcgtca ggatcagcaa
     1501 caacgcttac aacttctcct actcgccgaa cggcaagaag atcgtcttcg aggcaaccgt
     1561 gccctcggag gagcttgtcg tcatgaacgc gaacggcacg cggccgcggg tgatcaccgc
     1621 gaaggttcac aaatgcatcg gcaagcggca cccgacctgg tctccgaacg gcaaggagat
     1681 tgccttcgtc tgcctcaacc ggaacagcct ctccgatcac gacgtgtggt cggtcaacgc
     1741 caacggaacc ggcatccgcc ggatttccag gacccacgat gcctattccc cggcctggtc
     1801 gccgcgcgga aacaagatcg cctacacgac ctatggcggg tcgatctaca cggtcccggc
     1861 ccggggcggc aagagcaagc tgctcattgc ggaggctccc ggcggcgtgt tcggcggcac
     1921 cttccagggc gtcgactggt cgccgaacgg gcgccttctg gtctcggact cgagtggcga
     1981 cggcgtctac acgatcaacg cccggaccgg ggccacctcc ggcgatctcg cagaaatcgg
     2041 ggccgagccg gtcttctcgc ccgacgggaa gaagatcctc tacgtgggca tagcggagtc
     2101 caggggaagc accaggatcg gcctctggaa gatgaacctc gacggctcaa acaagcagca
     2161 ggtcacccgg ggcggctacg accgggcacc cagctgggca cccgcccgct aacggaatcg
     2221 gggtcggttc agcctggaac cggccccgtt cgcactgcct gaggggtgag cgacggggat
     2281 cgaacccgcg accgcctgga ccacaaccag gagctctacc aactgagcta cgcccaccgc
     2341 gaggggaaga aatctaccgg tcggctctcg gatacgctgc gccggtgccg cagccagcaa
     2401 gcccgggaga tgcgcgatga gtgtgcgccg cgaaatgcgg ggcttccttg tcggttcgct
     2461 cctctttgcc ctcggcgcga ctcccggcta tctcggactg gtcggcgtca ccgcggacaa
     2521 cctgacctac ttcctcggct cgatcttctt cacgattgcc ggcttcaccc agctgcggct
     2581 gaccggccgc tggcaacgcg gcggctggtc ggaccgcgac gcctgggccg actggtgggc
     2641 agcggcaatc cagtttctcg gcacccttgc cttcaacgtt tccaccttgg cagccctgct
     2701 cggcacgatc gatcccggca ccttcgagcg cctgggctgg cgccccgact tcatcggttc
     2761 gatctgcttc ctcgttgcct cggtgctcgc ggtcgaggcg accaccaacc gcgaatcgct
     2821 gtgggatccg gaagcccgca actggtggaa cacctggctg aacatggccg gctcggtcgc
     2881 cttcatgatc tcggcgatct ttgcctggat caatccctcc accggcgagc cgctgaatac
     2941 ggactgggcc aaccttggca ccttcttcgg agcgatcggc ttcttcgccg cggccgccct
     3001 gctcaagccc caggccgacg ccgactccaa cccttcaatg aacggcaccg gccccgccga
     3061 aaacggggat tagggctcga tcggggccgg ggcgcgggcc gaggaaagcg cgccggccga
     3121 ggccgagacg accagcgcga tcgccgcgat ctgggccgcg gacatgtcct ggccgaggag
     3181 caggaagccg atcgtcgcgg ccattgccgg ctcgaggctc atcatcaccc cgaagacgtt
     3241 cgagggcagg cggcggagcg cctccatctc gagtgagagc ggcagggtcg tgctgaggat
     3301 cgccaccgcg aagcccaccg ccaggaccga aggcagcagc aggtccgcgc cgccgtcggc
     3361 aaccccgaag ggcgtgcaga tgacggtcga gaagaccatc gcgatggcca gtccctgccc
     3421 gccgtagaac tcctccccca ccctcttgcc gagcaggatg taacagcccc agaacacacc
     3481 ggccaccacg gcgaggacga ccccgaagag ctcaatgcct tcttcgccga tgccaccgga
     3541 aagcagcagg atcccggccg ccgccatcgc gacccagatc aggtcccggc gccggtggga
     3601 ggtgatcagg gcaaccccga gtggcccgat gaactcgaag gtcacggcga tgccgagcgg
     3661 gatccggtcg atcgcctcgt agaaggagag gttcatcgca gccagcacga
//
LOCUS       DAOQFI010000089         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11396_length_3710_cov_3.315732, whole genome shotgun sequence.
ACCESSION   DAOQFI010000089 DAOQFI010000000
VERSION     DAOQFI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11396_length_3710_cov_3.315732"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PLE93_02445"
     CDS             complement(<1..1619)
                     /locus_tag="PLE93_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNH85938.1"
                     /translation="MKFRFSILVMALVAMAFSAAEASASPWIHAHRGGPLENGKAAQA
                     ENSSSGFAASIERGFVLEADVKLTSDKVPVIIHDDTLDRTTTCTGPVSAKTLDEIESE
                     CALDVKGIGDAATNLRAGYPGPTPRIPTLAAFLQVLKRTGAKANIEIKNLPTDNDWDP
                     TFEFAEIVANTVKNSGVPSSQLIIQSFLIDNLTTFKSIDPDPQTSYLTINLVNPIAIS
                     AARDNGIDWVSPEWPIDQAFVSDAHHAGLQVVPWTVDDAAGVREATRLGVDALITNDP
                     LMARAQVRKVAPPLAAIPKAPNLKSCNATFARDTRRPALAMLGRKDARGGPRVFAMQF
                     KQEARHIKTYASFRKKIECMIRKWVVPYKAKGRPNLVAFNEDVGLMTLGTGSRGAAAR
                     AAFAKPSSVTSCTGEAPPCRAIYSLMQVTAAYAGPDGEYTSRYSIPNPFTRGFVAAAD
                     TDARGWMQVFSDMARRYGIYIVGSNTQPRFRESQDPAEINLFRDPDLPKPKSVYVATG
                     PEVYNEAFMWGPKLVTQEGPRPLRNVVASNL"
     gene            1862..2056
                     /locus_tag="PLE93_02450"
     CDS             1862..2056
                     /locus_tag="PLE93_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85939.1"
                     /translation="MSRSSKKVPSCEDCYFRQKMLCALDLEGPCGTFRESRPEGLVPP
                     RQPMLLLRPPRWADRQIGAS"
     gene            2156..2947
                     /locus_tag="PLE93_02455"
     CDS             2156..2947
                     /locus_tag="PLE93_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH85940.1"
                     /translation="MKLTVLGKSPAWQDADGACSGYLVTEGDTTLLLDCGNGVFGKLR
                     KFVDYVDVDAIVISHHHVDHIIDLIPYGFALKFAPRQQPVPVDRWPGTGNPARPKLFV
                     PPGATGFFNRVSDSWGTGNLLEDAFDTAEYDPEGRLEIGPISISFTEVPHYITTYAMD
                     LTGPDGSRITYGADCCPNPALVELASGTDLLIAESTLPRPERTGVRGHLTPAEAGEHA
                     KLAGAGELILTHISDELDPGWAVEQASSVYEGPVQVAYEGLQVEV"
     gene            2981..3448
                     /locus_tag="PLE93_02460"
     CDS             2981..3448
                     /locus_tag="PLE93_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH85941.1"
                     /translation="MSTRDPFANFARMRREMDELLGGTWGRATYISRRSSGFSPNVDV
                     YYSGDPQRAVVKCDLAGIDLGSVGIEVSGRELTIVGERPVQETEGRVYQQVEIPTGPF
                     RYVVELQVDVDSERATATYDDGVLRVELPLKEEAEVTRRVPVDRSRKGRVIDG"
     gene            3441..>3710
                     /locus_tag="PLE93_02465"
     CDS             3441..>3710
                     /locus_tag="PLE93_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85942.1"
                     /translation="MAEPGESVVPVLEVVSGSDEEVEEALRGEQELPEAMPVLPLRDT
                     VAYPDTLAPLGVGQERSIKLVDDVLAGNRMLVMVASRDPEEDTPSP"
BASE COUNT          626 a         1167 c         1254 g          663 t
ORIGIN      
        1 ttcaggttgc tggcgaccac gttgcggagc ggccgcgggc cctcctgggt gaccagcttc
       61 ggcccccaca tgaaggcctc gttgtagacc tccgggccgg tcgccacgta aacgctcttc
      121 ggtttcggca ggtccgggtc ccggaacagg ttgatctcgg ccggatcctg cgattcacgg
      181 aacctcggct gggtgttgga accgactatg tagatcccgt agcgccgggc catgtcggag
      241 aaaacctgca tccagccgcg ggcgtcggtg tcggcggcgg cgacgaaacc gcgagtgaac
      301 ggattcggga tcgagtagcg cgaggtgtat tcgccgtccg gtcccgcgta ggcggcggtg
      361 acctgcatca gcgaatagat cgcccggcag ggcggcgcct caccggtgca ggaagtgacc
      421 gaagagggtt tggcgaaagc ggcgcgggcc gctgctcccc gtgaaccggt gccaagggtc
      481 atcagaccga cgtcctcgtt gaaggcgacc agattcggcc ggcccttcgc cttgtacggc
      541 acgacccact tcctgatcat gcattcgatc ttcttgcgga aactcgcgta ggtcttgatg
      601 tgccgggctt cctgcttgaa ctgcatcgcg aacacgcggg ggccgcccct ggcgtccttg
      661 cggcccagca tcgccagggc aggccggcgg gtgtcgcggg cgaaggtcgc gttgcatgac
      721 ttgaggttcg gagccttcgg gatcgcggcc agcggcggtg cgaccttcct gacctgggcc
      781 cgggccatca ggggatcgtt ggtgatcaac gcgtcgactc cgagcctggt cgcttccctg
      841 accccggccg cgtcgtcaac cgtccaggga acgacctgaa ggccggcgtg gtgggcgtcg
      901 gaaacgaagg cctggtcaat cggccactcc ggtgaaaccc agtcgatccc gttgtccctg
      961 gcggcgctga tcgcgatcgg gttgaccaga ttgatcgtca gatatgaagt ctggggatcg
     1021 gggtcgatcg acttgaacgt ggtcaggttg tcgatcagga aggactggat gatcagctgc
     1081 gaggaaggga ccccggagtt cttcaccgtg ttggcgacga tctccgcgaa ctcgaaggtg
     1141 gggtcccagt cgttgtcggt ggggaggttc ttgatctcga tgttggcctt ggctcccgtt
     1201 ctcttcaaaa cctggagaaa tgccgccagg gtggggatgc ggggagtcgg tcccgggtag
     1261 ccggctcgca ggttcgttgc ggcgtcgccg atccccttca catcgagcgc gcattcggac
     1321 tcgatttcgt caagggtctt ggccgaaacg gggccggtac aggtcgtggt gcggtcgagg
     1381 gtgtcgtcgt ggatgatcac cggcaccttg tccgaggtca gtttcacgtc ggcttcgagg
     1441 acgaatcccc gttcaatcga cgcggcgaag ccactgctcg agttctcggc ctgggccgcc
     1501 ttgccgtttt caagcgggcc gccgcggtga gcgtggatcc acggtgaagc cgatgcctcg
     1561 gccgcgctga aagccatcgc gacaagcgcc atgacgagaa tcgagaacct gaacttcact
     1621 tgaaccccct ctgtctccag tcgccccgcg ggcgcctctc cgtccgtgag gataaccgcg
     1681 gcggcgtccg ggcgggcgga tcgcccggtg catcattctg tccggaccct ggttcgcccc
     1741 ctcgggccgg gtcggcccgc cgcaaaatgc ggggcttgtt gcagacctgt gaaacaagaa
     1801 agctgcgatt tgcggaatcg ctgccgacgc gatccggcgg ggcttgcttg gatggcccgc
     1861 ggtgtcccgc tcaagcaaaa aggtcccctc ctgcgaagac tgctacttcc gccagaagat
     1921 gctctgcgca ctggatctcg aagggccctg cggcaccttc cgcgagagcc ggccggaagg
     1981 gctggttccg ccgcgccagc cgatgctgct gctgcgcccg ccccgttggg cagaccggca
     2041 aatcggcgcc agctaaccag gcggacggcg acgaacattc acggatggtt caccgggctc
     2101 cgctttcggt tgtccggagt agcatccgct tgatgaacgg aatcgggttc ctgtcatgaa
     2161 gctgaccgtg ctgggcaagt cgccggcgtg gcaggacgcg gacggggcat gcagcggcta
     2221 cctggtcacc gagggcgata cgacgctgct tctcgactgt ggcaacgggg tcttcggcaa
     2281 gctgaggaag ttcgtcgatt acgtggatgt cgatgcgatc gtcatttcgc accatcacgt
     2341 ggaccacatc atcgatctga tcccctacgg cttcgccttg aagttcgctc cccggcagca
     2401 accggtcccg gttgaccggt ggcccgggac cgggaacccg gcccggccga aactgttcgt
     2461 tccccccggg gcgaccggtt tcttcaaccg ggtttcggac agctggggta ccggcaacct
     2521 gctcgaggac gcgttcgaca cggccgaata cgaccccgaa ggcaggctcg agatcggccc
     2581 gatatcgatc agcttcaccg aagtccctca ctacataacc acctacgcga tggacctgac
     2641 cggcccggat ggttcccgca tcacctacgg cgccgactgc tgccccaacc cggccctggt
     2701 cgagctggca tccggcaccg acctgctgat cgccgagtcc acgctgcccc gtcccgagcg
     2761 gaccggagtc cgcgggcatc tgaccccggc cgaagcgggt gaacatgcga agctcgccgg
     2821 ggccggggaa ctcatcctca cccacatctc ggacgagctc gatcccggct gggccgtcga
     2881 acaggcgagt tccgtctacg aagggcccgt ccaggttgcc tacgagggtc ttcaagtcga
     2941 agtttgagcc ggaagtgctg cgttactaag cttcgaggtc atgtcaacgc gcgacccatt
     3001 tgccaacttt gcccggatgc gccgggagat ggacgaacta ctcggcggga cctggggccg
     3061 ggccacctac ataagccggc ggtcaagcgg cttttccccc aacgtcgacg tgtattacag
     3121 cggcgatccc cagcgggcgg tggtcaagtg cgacctggcc gggattgatc tgggctcggt
     3181 cgggatcgag gtcagcggac gcgaactgac gatcgtcggc gagcggccgg tccaggaaac
     3241 cgaaggccgg gtgtatcaac aggtcgagat accgaccggg cccttccgct acgtggtcga
     3301 actccaggtc gacgtcgatt cggagcgggc caccgccacc tatgacgacg gggtgttgcg
     3361 ggttgaactg ccactcaagg aagaggccga ggtgacccgg cgggttccgg tcgaccgttc
     3421 ccgcaagggg cgcgtgatcg atggctgaac cgggcgaaag cgtggtgccg gtgctcgaag
     3481 tcgtcagcgg cagcgacgag gaagtggaag aggcgcttcg cggcgagcag gaactgcccg
     3541 aggcgatgcc ggtcctgccc ctgcgggaca cggttgccta tccggacacc ctcgcgccgc
     3601 tcggagtcgg gcaggaaagg tcgatcaagc tggtcgacga tgtcctggcc ggcaaccgga
     3661 tgctggtgat ggtcgcatcc cgcgatcccg aagaggacac gcccagtccc
//
LOCUS       DAOQFI010000090         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11475_length_3697_cov_2.068918, whole genome shotgun sequence.
ACCESSION   DAOQFI010000090 DAOQFI010000000
VERSION     DAOQFI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11475_length_3697_cov_2.068918"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLE93_02470"
     CDS             complement(<1..486)
                     /locus_tag="PLE93_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85943.1"
                     /translation="MGARIIENGEIGISWVMEDEAMARASHALTDGEKVWLVDPVDDD
                     AAIERALTLGKPAVVFQLLDRHNRDCAQVADRLGIDHVALPLELRGTPFEAIEVVENR
                     IWKERALWWKKTKTLVVAEAVGASRMFNPSEAGAGVHIGLRATPPRKQLGTYNPRHLL
                     MG"
     gene            548..2251
                     /locus_tag="PLE93_02475"
     CDS             548..2251
                     /locus_tag="PLE93_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85944.1"
                     /translation="MRGVKQSVNSGGVLWLAALVTCFALLPAAKSEAIPIVKNVQLAG
                     SGGQWKLLVPMTLPLEVSGFEVTGKAGVELPGGQWTKTWQTRVHSGSLREADQRDFFL
                     HVHGIPVSRKLARRILRAGKEASAKVTVRFTGPSADPEIRPDGSLGLSQKGIPRARPG
                     VCETLPRVLLEGSKIRSRTLRFPVCGKDLSWELVQRPLKGDYLSRADVFRYSRRSGQV
                     GADELELAGSFRGRQVTTQRIQFRLGNVPASSVSVAAFGDSVTAGFGYFGATGKPMTL
                     GQLLDCKPGATTLNDACSSNSYNRNSSVGSKPNYLQDFGLSRNISWAAQWANEYGITE
                     YENYAVTGSAPADWLPNGQFHQTLQNIESQNPDYILMTLGANPLLDDVLFDVDTMGCA
                     LESDLFGDFRQCVLDAFASVNLDSNMNQIFTDLVQNSSSHVVLMGYPFTIPSSALAYS
                     AAQLEMMESLLNEVIADEAESVSSSRITVVTPPRFNVGIDMEPLYPSNYSCSLLGYKV
                     DGPSVQSTPTQDELEIDHPLSFCSGPSFGKPWVISGDTGIHPSAAGYLQMSSQIPAPG
                     S"
     gene            2293..2955
                     /gene="msrA"
                     /locus_tag="PLE93_02480"
     CDS             2293..2955
                     /gene="msrA"
                     /locus_tag="PLE93_02480"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNH85945.1"
                     /translation="MFFGRDKTTMPAPEDSLPGRSGYAFPVPDNHTVLGTPIQGPFPE
                     GIEVAHFALGCFWGAERLFWNLDGVYTTAVGYQNGFTPFPTYEEVCSGKTGHAEAVLV
                     AFDPEKVSYERLLEVFFSEHDPTQGMRQGNDVGTQYRSAVFTVGDRQKEIAEMARDTY
                     QRALNERGLGEITTEIEEAGPFYYAEGPHQQYLDKVPNGYCGLAGTGVACQVPVAGGS
                     ST"
     gene            3038..>3697
                     /locus_tag="PLE93_02485"
     CDS             3038..>3697
                     /locus_tag="PLE93_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85946.1"
                     /translation="MTPSLASYIAGTLQIAVVVGAVWYGSWWLRRWIVPEFSGALARL
                     AELTIAFALIILSLELIGSVGLLKEVWITVACVVIPLIGAGLAWKFAPRDVEEIAAPA
                     VPTWAFLVAVAVASWCVAEWAFPTQLSLDQGMFGGDTTWYHMPAAARFIQDSSIIPLH
                     FTDALRLAVWFYPQNSELMHGALMGVMGSDWLSPVFNMLPLGIALLACWCVGRPYGVG
                     PA"
BASE COUNT          620 a         1251 c         1161 g          665 t
ORIGIN      
        1 ccccatcaac aaatgcctcg ggttgtaggt gccgagctgc ttgcggggcg gggtggcgcg
       61 caggccgatg tgaacgccgg cgccggcctc gctcggattg aacatcctgc tggccccgac
      121 cgcttcggcc acgaccaggg tcttggtctt cttccaccag agcgcccgtt ccttccagat
      181 ccggttctcg accacctcga tcgcctcgaa aggagtgccc cggagttcca gcggcagggc
      241 gacatggtcg atgcccagcc ggtcagctac ctgcgcacag tcgcggttgt gccggtcaag
      301 caactggaag accacggccg gcttgccgag ggtcagggcc cgctcgatcg cggcgtcgtc
      361 atcgaccgga tcaacaagcc agaccttctc gccatcggtg agggcatggg aagcccgggc
      421 catcgcctcg tcctccatga cccagctgat cccgatctcg ccgttctcga tgatcctcgc
      481 gcccacgggc gggagtctag acccgcagcc gctccgatcg gccgcaccgg ggtttaaggt
      541 atgcgaaatg cggggagtga agcagagcgt caattcgggc ggggtgctgt ggctggcggc
      601 gctggtcacc tgcttcgccc tcctgcccgc cgccaagtcg gaagcgatac cgatcgtgaa
      661 aaacgtccag ctggccggaa gcgggggaca gtggaaactg ctcgtcccca tgaccctccc
      721 gctcgaagtc tcggggttcg aagtgacggg gaaggcaggg gtcgaactcc cgggcgggca
      781 gtggacgaag acctggcaga cgcgagtcca ttcgggatcg ctccgggaag ccgaccagcg
      841 cgacttcttc ctccacgtcc acggaattcc cgtttcgagg aagctcgcgc gccggatcct
      901 ccgggccggc aaggaagctt ccgcgaaagt cacggtcagg ttcaccgggc cctccgctga
      961 tccggaaatc cgacccgacg ggtctcttgg cctgtcgcag aaaggcatcc cgcgggcgcg
     1021 gcccggggtc tgcgaaacac tgcccagggt tctcctcgaa ggcagcaaga tcagatcacg
     1081 gacgctccgt ttccccgttt gcggaaagga tctctcctgg gaactcgtcc agcgacctct
     1141 caagggcgac tacctgagca gggcggacgt gttccgctac tcgcggaggt ccggtcaggt
     1201 cggtgccgac gaactggaac tcgccggctc tttccggggg cgccaggtga cgacccagcg
     1261 gattcagttc cgtctcggca acgtgccggc atccagtgtc tcggtcgcgg cgttcggcga
     1321 ctcggtgacc gccggcttcg ggtatttcgg ggcgaccggc aagccgatga ccctcgggca
     1381 actgctcgac tgcaaaccgg gagctacgac cctgaacgac gcctgctcct cgaactccta
     1441 caaccgcaac agctcggtcg ggagcaagcc gaactatctg caggacttcg ggctctcgcg
     1501 gaacatttcc tgggccgcgc aatgggcaaa cgaatacggc atcaccgaat acgagaatta
     1561 cgcggtgacc ggttccgcgc cggccgactg gctgccgaac ggtcagttcc atcagaccct
     1621 ccagaacatc gagtcgcaga acccggacta catcctgatg acgctcgggg ccaatccgct
     1681 gctcgatgac gtgctctttg acgttgacac catgggctgc gctctggagt cggatctctt
     1741 cggcgacttc cgccaatgcg tcctcgacgc cttcgcctcg gtcaacctcg actcgaacat
     1801 gaaccagatc ttcacggacc tggtccagaa ctcgagttcg catgtggtgc tgatgggtta
     1861 cccgttcacg attccatcct cggcactcgc ctacagcgcg gcccagctcg agatgatgga
     1921 atcgctgctc aacgaggtga tcgcggacga ggccgaatcg gtctcgagca gccggatcac
     1981 cgtggtgacc ccgccccgct tcaacgtcgg aatcgacatg gagccgctct acccctcgaa
     2041 ctattcctgt tcgctgctcg gctacaaggt cgatggcccg agcgttcagt cgaccccgac
     2101 ccaggatgaa ctggagatcg accacccgct ctccttctgt tccggaccct cgttcgggaa
     2161 gccctgggtg atcagcggcg acaccgggat ccacccgagt gccgccggct acctgcagat
     2221 gtccagccag attcccgcgc ccgggtcctg aagctcgcga accgggaagg gcacccgagt
     2281 agcatcggtt ccatgttctt cggtcgcgac aaaaccacga tgcccgcccc ggaagattca
     2341 ctcccgggcc gctccggcta cgccttcccg gttccggaca atcacaccgt tctgggaacc
     2401 ccgatccagg gacccttccc ggaagggatc gaggtcgccc atttcgcgct cggctgcttc
     2461 tggggcgcgg aacggctttt ctggaacctc gacggtgtct acacgaccgc ggtcggctac
     2521 cagaacgggt ttacgccctt cccgacctac gaagaggtct gctccgggaa gaccggccac
     2581 gccgaggcgg tgctggtcgc cttcgatccg gagaaggtca gctacgagcg gctgctcgag
     2641 gtcttcttct ccgagcacga cccgacccag gggatgcgtc agggaaatga cgtcggaacc
     2701 cagtaccgct ccgccgtctt cacggtcggc gaccggcaga aggagatcgc cgagatggcc
     2761 cgcgacacct atcagcgggc actgaacgag cggggtctgg gcgagatcac gaccgaaatc
     2821 gaagaggccg gccccttcta ttacgcggag ggcccgcatc agcagtacct ggacaaggtc
     2881 ccgaacggct attgcggcct ggctggcacc ggggtcgcat gccaggtgcc ggtggccggc
     2941 ggttcctcca cctgagccgc cggggatccg ccgggaaccg tttcgcccgg ccataaatta
     3001 ggattcccgc ggtgccaacc ctgctcacag gacggctttg accccgtccc tagcctcata
     3061 catcgccgga acgctccaga tcgccgtcgt ggtcggcgcc gtctggtacg gatcctggtg
     3121 gcttcggcga tggatcgtgc ccgaattctc gggtgcgctg gcgcgcctcg ccgagctgac
     3181 gatcgccttc gcgctgatca tcctctcgct cgagctgatc ggttcggtcg gcttgctgaa
     3241 ggaggtctgg atcaccgtcg cctgcgtcgt gatccccctg atcggcgccg gcctggcctg
     3301 gaagttcgcg ccgcgagacg ttgaggagat tgccgcgcca gcggtcccca cctgggcctt
     3361 cctggtcgcg gtcgcggtgg cgtcctggtg tgtggcggaa tgggctttcc cgacccagct
     3421 cagcctcgac cagggcatgt tcggtggtga cacgacctgg taccacatgc cggccgcggc
     3481 gaggttcatt caggactcct cgatcatccc gctccacttc accgacgcgc tgcggctggc
     3541 ggtctggttt tacccccaga actcggagct gatgcatggc gccctgatgg gcgtgatggg
     3601 cagcgactgg ctttccccgg tcttcaacat gctccccctc gggatcgccc tgctcgcctg
     3661 ctggtgcgtg ggccggcctt acggggtagg gcccgcc
//
LOCUS       DAOQFI010000091         3696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11477_length_3696_cov_4.233178, whole genome shotgun sequence.
ACCESSION   DAOQFI010000091 DAOQFI010000000
VERSION     DAOQFI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11477_length_3696_cov_4.233178"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLE93_02490"
     CDS             <1..447
                     /locus_tag="PLE93_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85947.1"
                     /translation="IVFDHSDPWITLLRRFSFLPAIPISGNGKSRYQPVWADDVARAV
                     ENSLKGDGDGDHLYELAGPQTMTYAEMSELVGLLAGRPRPVVRIPLQLVHLGLSAIHR
                     LVGENAFATWQEAELMRVSMVTPRGSEDLESLGVQPRSMADVLADS"
     gene            482..1525
                     /locus_tag="PLE93_02495"
     CDS             482..1525
                     /locus_tag="PLE93_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85948.1"
                     /translation="MRFPARLSLATVVVVFALAAVADAGVFLPPKGKVYGGVSDNGTY
                     RGFENFSEATGKHPAVLQTYHPWGNQLFLAYQRWQNTEVLPMLHISTADDETRAELIT
                     PKEIARGVGDPYLIEINRFFSARHQKAFIRPLGEPNRCLNPYTAFTCSGKRRGGEHKT
                     IWYRRAFRRIALITRGGTTAGRIDRNLKKLQLPKLNWGSLTRSPKLPKAPVSIVWSTL
                     PSGSPSVRGNHPVDYWPGSKYVDWVGTDFYSNYPYWRDLNRFVHNRRWRQKPVALTEW
                     GVVGADDTRFARRVFAWVKQQPRVRMMVYYRGFGTADPYYPYNYPLTVKVIRNKLKSP
                     RFLAFAHGYARRR"
     gene            complement(1522..2553)
                     /locus_tag="PLE93_02500"
     CDS             complement(1522..2553)
                     /locus_tag="PLE93_02500"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HNH85949.1"
                     /translation="MDVTTRRLLAQGLIEPAAATPGQVVETLLAVQAQDGRGARLAIR
                     PRLSERVTEPSASMVDAALDEGSLVVNWLNRGTLHLVRAEDHGWLHALTAPRQATANQ
                     TRLRQEGVSEKQADRGVGMIVDRLKDGPATRGELKEVLAAEGVPVAGQALVHILLFAS
                     LQGLILRGPTLGGEHAFVLVEDWLGPQGEVDLELAAAELARRYLTGHGPATDRDLARW
                     AGVNLGLARKGLAAIAGETVEADGCLELKEKPAPTVAEAPVRLLGAFDPVLHGWTDRA
                     WILPDEETRKVVTTNGIFRPTILAGDRIVGTWTMPSGRVELAPFGKLDRETVAALEVE
                     AEMVTRYLG"
     gene            2602..3327
                     /locus_tag="PLE93_02505"
     CDS             2602..3327
                     /locus_tag="PLE93_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85950.1"
                     /translation="MKSEGFRRHLTYSNVMATIAVFAVLGGGAYAATKIPKNSVGARQ
                     LKKNAVTTAKLKNGAVTSAKVKKGSLLASNFKSGQLPRGATGPTGATGAAAVTVAAHG
                     GGEDPPALFGPTTNIVMASAPISLPRESDVVITGSMGDKQVECSVDGCQGPGGTDSTI
                     VGLYLEFPNSTGPSPVPGGGISVLGSSFNRIQIPPLSGVIRDIPPGIYTAKLILAGLG
                     ISTSVFDNGGADPWITALATDSG"
     gene            complement(3453..>3696)
                     /locus_tag="PLE93_02510"
     CDS             complement(3453..>3696)
                     /locus_tag="PLE93_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNH85951.1"
                     /translation="DNKVRELKEGEGLNAANGEYGDMVKAGVLDPTVVTRSALQNAAS
                     IAKNILVTEAIIAEPPEEGGAGMPAMPDMGGMGGMM"
BASE COUNT          605 a         1268 c         1168 g          655 t
ORIGIN      
        1 atcgtcttcg atcattccga cccctggatc accctgctgc gccgcttctc cttcctgccg
       61 gcgattccga tctccggcaa cggcaagtcc cgctaccagc cggtctgggc tgacgatgtc
      121 gcccgggcgg tcgagaactc gctcaagggg gacggcgatg gcgaccacct gtacgaactc
      181 gccggcccgc agacgatgac ctacgccgag atgtccgagc tggtcgggct gctggccggt
      241 cggccccggc cggtggtccg gattccgctg cagctggtcc acctgggtct ttcggcaatc
      301 caccggctgg tcggtgaaaa cgccttcgcg acctggcagg aggccgagct gatgcgcgtc
      361 tcgatggtca ctccccgggg cagcgaggac ctcgagtcac tgggagtcca accgcgcagc
      421 atggctgacg ttctcgccga ttcctgaccg tcccgggcgc gggctgcgat catttctggc
      481 agtgaggttt ccggcccgtc tcagcctggc gacggtcgtc gtcgttttcg ctcttgccgc
      541 agtcgccgac gcgggcgtct tcctgccgcc gaagggcaag gtttacggcg gggtaagcga
      601 caacggcacc taccgcggtt tcgagaactt ctccgaagcg accggcaagc acccggcggt
      661 ccttcagacc taccacccgt ggggaaacca gctcttcctc gcctaccagc gctggcagaa
      721 caccgaggtg ctgccgatgc tccacatctc gacggccgac gatgagacga gggcagagtt
      781 gatcaccccg aaggagatcg cccggggcgt cggcgacccc tatctgatcg agatcaaccg
      841 gttcttctcg gccaggcacc agaaggcctt catcaggccg ctgggtgaac cgaaccgctg
      901 cctcaatccc tacaccgcct tcacctgttc ggggaagcgg cgcggcggcg agcacaagac
      961 gatctggtac cggcgcgcgt ttcgccggat cgccctgatc acccggggcg gcaccacggc
     1021 gggccggatc gaccgcaacc tgaagaagct ccaactgccg aagctgaact ggggctcgct
     1081 gaccaggagc ccgaagctgc ccaaggctcc ggtgtcgatc gtctggagca cccttccgtc
     1141 gggctccccg tcggtccggg gcaatcatcc ggtcgactac tggccgggaa gcaagtacgt
     1201 cgactgggtc ggcactgact tctattcgaa ctatccctac tggcgggacc tcaaccgctt
     1261 cgtccacaat cgccgctggc gacagaagcc cgtcgccctg accgagtggg gcgtggtcgg
     1321 ggccgacgac acccgcttcg cccgccgggt gttcgcctgg gtgaagcagc agccgcgggt
     1381 ccggatgatg gtctactacc gcggcttcgg aaccgccgac ccctactacc cgtacaacta
     1441 cccgctcacg gtcaaggtga tccgcaacaa gctgaagagt ccccgctttc tcgccttcgc
     1501 ccacgggtac gcgaggcgcc gctaaccgag atagcgcgtc accatttcgg cttcgacttc
     1561 gagagctgcc accgtctccc gatcaagctt tccgaagggt gcgagttcaa ccctgccgga
     1621 gggcatagtc caggtaccga cgatccggtc gccggcaagg atggtcggcc ggaagatccc
     1681 gttggtggtg acgaccttcc gggtctcttc gtccgggagg atccaggcgc ggtcagtcca
     1741 gccgtggagg acgggatcga aggcaccgag cagcctcacc ggcgcctcgg ccaccgttgg
     1801 cgccggtttt tctttcagtt cgaggcatcc gtccgcttcc actgtttcgc cggcgatcgc
     1861 cgccaggccc ttgcgggcca gaccgaggtt gactccggcc cacctggcga ggtcacgatc
     1921 ggttgccggt ccgtggcccg tgaggtaccg gcgcgccagt tcggcggcgg ccagttcgag
     1981 gtcaacctcg ccctgcggcc cgagccagtc ttcgaccaga acgaaggcat gctcgcctcc
     2041 gagcgtcggt ccgcgcagga tcagaccctg aagggaggcg aacagcagta tgtgaaccag
     2101 cgcctggccg gcgaccggca ctccctccgc cgcgagaact tccttcagct ctccgcgggt
     2161 ggccggcccg tccttcagcc ggtcgacgat catcccgact ccccggtccg cctgcttctc
     2221 cgagacgcct tcctgcctca ggcgggtctg gttcgcggtg gcttgccggg gcgcggtcag
     2281 cgcgtgcagc cagccgtggt cttcggcccg aaccaggtgg agggtgccgc ggttcagcca
     2341 gttgacgacc agcgacccct cgtcgagcgc ggcatcgacc atcgaggccg atggttccgt
     2401 gacccgctcg ctcagccgtg gccggatcgc cagcctggct ccccggccgt cctgggcctg
     2461 tacggcgagc agcgtttcga ctacctgacc gggggtcgcc gcagccggct cgatcagacc
     2521 ctgggcaagc agccgtctgg ttgtcacgtc cacgggtcaa ggctatggtg gattcacggt
     2581 tgaacgactt gagggggagc gatgaagtcc gaaggattcc ggcggcacct gacctattcg
     2641 aacgtgatgg ccactatcgc cgtgtttgcg gtcctcggtg gcggcgccta tgcggcaacg
     2701 aagattccga agaacagcgt cggagccagg cagctcaaga agaacgccgt caccaccgcc
     2761 aagctgaaga acggtgcggt cacctcggcc aaggtgaaga agggctcgct gctcgcttcg
     2821 aatttcaagt ccggtcagct gccacgcgga gcgaccggac cgaccggcgc gaccggtgcc
     2881 gccgcggtca ccgtggcggc gcatggaggc ggtgaggatc cgcccgccct gttcggcccg
     2941 accacgaaca tcgtgatggc ctccgcgccg atcagtctcc ccagggagag tgacgtggtg
     3001 attacgggtt cgatgggtga caagcaagtc gagtgttcgg tcgacgggtg ccaggggccc
     3061 ggaggaaccg attcgacgat cgtcggcctc tatctggaat tccctaactc gaccggaccc
     3121 tccccggtcc ccggcggcgg aatctcggtt ctgggcagca gcttcaaccg aatccagatt
     3181 cccccgctct ccggggtgat ccgggatatc ccgcccggca tttacacggc caagctgatc
     3241 ctcgccggcc tcggaataag cacttccgtc ttcgacaacg gcggcgccga cccctggatc
     3301 accgccctcg ccacggactc cggctaggag gcatgcatct gggttgaagt tatccgcaca
     3361 tggaacgaat aacttcaacc cagatgccca tgcaatgaaa aaggcccggg atttctcccg
     3421 ggcccttctc ggaaatctct gaagagattt ctttacatca tgccgcccat tccgcccatg
     3481 tcgggcattg cgggcatgcc ggcgccacct tcctccggcg gctcggcgat gatcgcctcg
     3541 gtcaccagga tgttcttggc gatcgacgcg gcgttctgga gcgcggagcg ggtcacgacg
     3601 gtcgggtcga gcacgcccgc cttgaccatg tcaccgtact cgccgttggc tgcgttcagg
     3661 ccttcgcctt ccttcagctc gcggaccttg ttgtcc
//
LOCUS       DAOQFI010000092         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11675_length_3662_cov_2.837261, whole genome shotgun sequence.
ACCESSION   DAOQFI010000092 DAOQFI010000000
VERSION     DAOQFI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11675_length_3662_cov_2.837261"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..647)
                     /gene="hisIE"
                     /locus_tag="PLE93_02515"
     CDS             complement(30..647)
                     /gene="hisIE"
                     /locus_tag="PLE93_02515"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNH85952.1"
                     /translation="MPEVEVKFDRDGLVPCIAQNAETGEVLTLAYMNEESLRLTRESG
                     LVTYFSRSRQKIWQKGEESGNVQKVRQLRIDCDGDAIVALVEPSGPACHTGERTCFFR
                     DLETGANPEPVSGEALSVLARTIDSRLAEKPEGSYVVKLLEDPAFAGEKVMEEAEEVS
                     RAVREETDDRVANEAADVIFHLAALIRSRGITLDDVGEVLNGRRA"
     gene            complement(647..1078)
                     /locus_tag="PLE93_02520"
     CDS             complement(647..1078)
                     /locus_tag="PLE93_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85953.1"
                     /translation="MLRKVAICALIGSATLVSACGGSDDSTSSEDSQAITELVAKLNQ
                     ATQEKDASAFCLIMQPSAVEQTFHDIDRCVSETTPILKAAGDQGVLKIDTIEVNGDIA
                     AVTFQGGTSREAKFVKEDGQWYVPLSQSEITTNQGSNGSEG"
     gene            1149..2099
                     /locus_tag="PLE93_02525"
     CDS             1149..2099
                     /locus_tag="PLE93_02525"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNH85954.1"
                     /translation="MKWKRGRSGNRNVIDIRGAKRGGGGGGGGFGGIGGMGGGGMQLP
                     GGKGKIGGLVGVIIVIIVIGTQVLGGGGSGGGTGFDMGSMFPGMGAVPGAENPSPIPA
                     GEDPDADLKDFSAYVFTDAQQVWRKAFTRDGRRWQDAQLVLYSGAVSTGGCGNATSAV
                     GPFYCPADNRVYLDLSFYKDMKNQLGADGDFAWAYVIAHEMGHHVQNMGGISDEVSRE
                     KREQSESRNEISVRNELQADCLAGVWASTVFDQLEAGDLDEAIGASEAVGDDRLQSRG
                     GGSVNPDSFTHGTSAQRHRWFKTGFESGDPASCDTFSGDI"
     gene            complement(2354..3262)
                     /gene="era"
                     /locus_tag="PLE93_02530"
     CDS             complement(2354..3262)
                     /gene="era"
                     /locus_tag="PLE93_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNH85955.1"
                     /translation="MSEGSRSGFVGLAGRPNVGKSTLVNAIVGAKVAISSVRPQTTRR
                     AIRGVATDLEAGTQLVLVDLPGVQRPRDELTSRMQKTVESELAGADVALLVINGEEGV
                     GPGDRFIAETLLGARAKAGGLPIICAVNKADRLGRNIVPVLVAAAELAGVDEVFPVSA
                     LTGQGVPELASYLASIVPEGPYLYPPEDHTDQPRELLLAELIRGQALIRTRDEIPHSV
                     EVRVTGIEQREDGLWLVDAEIWVESDSQKRILIGKKGFKVGEIGTGARKDIEGELGGK
                     VHLDLQVKVKRKWRRDEDMLDRLGID"
     gene            complement(3255..>3662)
                     /gene="ybeY"
                     /locus_tag="PLE93_02535"
     CDS             complement(3255..>3662)
                     /gene="ybeY"
                     /locus_tag="PLE93_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNH85956.1"
                     /translation="ERQVSLDLDDVPARFRPAVTATLEAAGVGNGHLAVEVVDEARIR
                     KLNRDHRGKNKPTDVLSFPVDGEEGPGPVELGDVVICPEFCVDEVEAVVHGVLHLCGY
                     DHETDQGEMLDLQDRVVSGLGIEPKMTVEADDE"
BASE COUNT          578 a         1227 c         1190 g          667 t
ORIGIN      
        1 caagatccgg ggtgagccgc gaagcgaggt caggcgcgac ggccattcag gacctccccc
       61 acgtcatcga gggtgatgcc gcgcgatcgg atcagcgccg ccaggtgaaa gatcacgtcg
      121 gccgcctcgt tggccacccg gtcgtccgtc tcctcacgga cggcccggct cacctcttcg
      181 gcttcctcca tcaccttctc gccggcgaag gcgggatcct cgagcagctt caccacgtag
      241 cttccctccg gcttctcggc gaggcgggaa tcaatcgttc gggccagcac cgaaagggct
      301 tcgccactga ccggttcggg gttcgcgccg gtttcgaggt cgcggaaaaa gcaggtgcgc
      361 tcaccggtgt ggcaggcggg gccggacggt tcgaccaggg cgacgatcgc gtcgccgtcg
      421 cagtcgatcc ggagttggcg gactttctgc acgttgccgg attcctcgcc cttctgccag
      481 atcttctggc gcgaacggct gaagtaggta accagaccgg actcgcgggt cagcctcagg
      541 gattcctcgt tcatgtaggc gagggtcagc acctcgcccg tctccgcgtt ctgggcgatg
      601 caggggacca gcccgtcccg gtcaaacttc acttcgactt ccggcatcag ccttcgcttc
      661 cgttcgagcc ctggttggtc gtgatctcac tctggctgag cggcacgtac cactggccgt
      721 cttccttgac gaacttcgct tcgcggctgg tgccgccctg gaacgtgacc gcggcgatgt
      781 cgccattcac ttcgatcgtg tcgatcttga ggacaccctg gtcaccggcc gccttgagga
      841 tcggggtcgt ttcactcacg caacggtcga tgtcatgaaa ggtctgctcg accgcactgg
      901 gctgcatgat caggcagaag gccgaggcat ccttttcctg ggtcgcctga ttcagcttgg
      961 cgacgagttc ggtgatcgcc tgactgtcct cgctcgaagt cgagtcatcg gacccgccgc
     1021 aggcggagac cagcgttgcc gatccgatca gggcgcagat tgcgactttt cgcagcatgg
     1081 cccaatccta cgaaagcctg accgggatcc ggtcgcacga tgccggcttg ccgtctaggg
     1141 ttcaggccat gaagtggaag cgaggccgtt caggcaaccg gaacgtgatc gatatccgcg
     1201 gcgcgaagag gggcggcgga ggcggcggtg gtggcttcgg cggcatcggt ggcatgggcg
     1261 gtggcggcat gcagctgccg ggcggcaagg gcaagatcgg cggcctggtc ggcgtgatca
     1321 tcgtgatcat cgtgatcggc acccaggttc taggcggggg cggctccggt ggcggcaccg
     1381 gcttcgacat gggatcgatg ttcccgggaa tgggcgccgt gcccggcgcg gagaatccct
     1441 ccccgatccc cgccggcgag gacccggatg ccgacctcaa ggatttctcg gcctacgtct
     1501 tcaccgacgc ccagcaggtc tggcgcaagg ccttcacccg ggacggccgg cgctggcagg
     1561 acgcgcagct ggtgctctac tcgggcgcgg tcagcaccgg tggctgcggc aacgccactt
     1621 cggcggtcgg ccccttctac tgcccggccg acaaccgggt ctacctggat ctgagcttct
     1681 acaaggacat gaagaaccag ctcggggccg acggcgactt cgcctgggcc tacgtgatcg
     1741 cccacgagat gggccatcat gtccagaaca tgggtggcat ctccgacgag gtttcccgcg
     1801 agaagcgtga gcaatccgag tcacgcaacg agatctcggt ccgtaacgag cttcaggccg
     1861 actgcctcgc cggagtctgg gcgtcaaccg tcttcgacca gctcgaggcc ggcgacctgg
     1921 acgaggcgat cggcgcctcc gaagcggtcg gtgacgaccg cctccagtcc cgcggcggcg
     1981 gcagcgtcaa cccggactcg ttcacccacg gaacttccgc ccagcgccac agatggttca
     2041 agaccggctt cgagtcggga gatccagcca gctgcgacac gttctcgggc gacatctagc
     2101 ctgattcgcc cggtggttac gccgcggggt gggggagctt ccccgccgta cctccagggc
     2161 cgggttcagg ctctggtttc gggaggtgcc gcacattcag gcccttgatg ggcctatttt
     2221 tgcggcgcct atcccggatc cggccggata tcgatcgaga tcggagttgg gtgcgctata
     2281 tggcagaaaa cgggatctcg aaaagccaac accaccccac ttcagcggct ggtggttcct
     2341 ggcgccaccc gggctaatca atgccgaggc ggtccagcat gtcttcgtcg cggcgccact
     2401 ttcgcttgac cttgacctgg aggtcgaggt ggaccttgcc gccgagttcc ccttcgatgt
     2461 ccttgcgggc cccggtgccg atctctccga ccttgaagcc cttcttgccg atcaggatcc
     2521 gcttctggga gtccgattca acccagatct cggcgtcgac cagccagagg ccgtcttcgc
     2581 gctgctcgat cccggtcacc cgcacctcaa ccgaatgggg aatctcgtcc cgggtacgga
     2641 tcagggcctg gccccggatc agttcggcca gaagcagttc ccggggctga tcggtgtgat
     2701 cctccggcgg gtagaggtaa ggcccctcgg ggacgatcga ggccaggtac gaggcgagtt
     2761 ccggcacgcc ctggccggtc agggcactga ccgggaaaac ctcgtcgaca ccggcgagct
     2821 ccgcggcggc gaccaagacc ggcacgatgt tccggccgag ccggtcggcc ttgttgacgg
     2881 cgcagatgat tggcaagccg ccggctttgg cccgggcccc gagcagcgtt tcggcgatga
     2941 accggtcgcc cggcccgacg ccttcctcgc cattgatcac cagcagggcg acatcggccc
     3001 ccgccagttc gctttcgacc gtcttctgca tgcggctggt caactcgtcc cggggccgct
     3061 ggaccccggg cagatcgacc agaaccagct gggtgccggc ctcgaggtcg gtcgccactc
     3121 cccggatcgc ccggcgcgtg gtctggggcc ggaccgagga gatcgcgacc ttggccccga
     3181 cgatcgcgtt gaccagggtc gacttgccga cgttcggcct tccggccagt ccgacgaaac
     3241 ccgagcggct gccttcactc atcgtcagcc tcgacggtca tcttcggttc gatccccagc
     3301 ccggaaacga cgcggtcctg gaggtcgagc atctcgcctt gatcggtttc gtggtcgtaa
     3361 ccgcagaggt ggagcactcc gtggacgacc gcttccacct cgtcgacgca gaattcgggg
     3421 cagatgacca cgtcccccag ctcaaccggg ccgggaccct cttccccgtc gaccgggaag
     3481 gaaaggacat cggtcggttt gttcttgccg cggtggtccc ggttcagttt ccggatcctg
     3541 gcttcatcca cgacctccac cgccaggtgg ccattcccga cccctgccgc ttcgagtgtc
     3601 gcggtcacgg ccggccggaa acgggccggc acgtcatcaa ggtcaaggct cacttgccgt
     3661 tc
//
LOCUS       DAOQFI010000093         3656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11714_length_3656_cov_3.474590, whole genome shotgun sequence.
ACCESSION   DAOQFI010000093 DAOQFI010000000
VERSION     DAOQFI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11714_length_3656_cov_3.474590"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1012)
                     /locus_tag="PLE93_02540"
     CDS             complement(98..1012)
                     /locus_tag="PLE93_02540"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNH85957.1"
                     /translation="MGKIFFFTGEALRAIKRNVAPTTAAVVTTVLTALLLGVLIPVFQ
                     TAQSKGEDVRGQLELRTFLYDDATKAETQALMVKLEGLPHVESVTLLTKADAKKEFLA
                     SDFGKQNPDALKQLGGKNPLPANFVIKPDDASNLEGIRNAIQPVGAKGDPQYISPIIA
                     SVDDRQEDARDIEQVTGALKLILTVITVLLICASLLLIGNTIRLSIYTRRREIEVMRL
                     VGATRWFIRWPFMIEGVVVGILGGAIAILVLWIGKVTVVDPLSDKFALMNAQDSMSFA
                     ALVGCLFIAAILVSAIGSGLTMRRFLKV"
     gene            complement(1012..1893)
                     /gene="ftsE"
                     /locus_tag="PLE93_02545"
     CDS             complement(1012..1893)
                     /gene="ftsE"
                     /locus_tag="PLE93_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNH85958.1"
                     /translation="MAFGKKKRESAGAELEAARARKQAERPRKGGAPIIEMRDVSKVY
                     PGGHLAVDRVSLAINRGEFVFLVGPTGCGKSTLIKMLIRELEPTDGTISIAGRDINQL
                     SEKKIPQLRRNIGTVFQDFKLLPSRTVYDNVAYALQVIGASRAEIRQKVPETLRLVGL
                     STKLHNYPDELSGGEQQRVSVARAFVNHPPLLLADEPSGNLDPVTSVGIMQLLYRINK
                     TGTTVVVVTHDREMVDRMRRRVVELYEGRVIRDEVAGGYTEESTTEFGVRMRAEMGVG
                     VEGHTNGNSGNSGKGAH"
     gene            2057..3217
                     /locus_tag="PLE93_02550"
     CDS             2057..3217
                     /locus_tag="PLE93_02550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNH85959.1"
                     /translation="MSRAAHLANRAALAGCGLLLAILLAAFLLPSSPAAAGGEPSSSE
                     SGATASIVNGKATTIGQWPWQVALTVSSRIAPRASTSWRFFCGGSVLAPRLVITAGHC
                     VADLNRGQIRKIEIVSGRTRLNSNRGQVARVTGLRMPVNASGKRRFRGLAGAADWDVA
                     LLTLASPLSAEPIKLAGPDEAAAWSPGQVAWTTGWGITKAFSQRVPARLQVARQVLMG
                     NGLCHRADGVAYKSRRMICMGAPRAHASACNGDSGGPLVVETGAGYRLVGLTSYGDGA
                     CRGFVPSVDTRVSGRQIRNWVARTAMNLTGRNVVGSGGTPRPAPDWCKVPDVFRLRQS
                     QAKARLEAAGCRLGRVRTDYYSVGQAGRIVGSRLAPGWLAPPGFKVGVWVAP"
     gene            3214..3504
                     /locus_tag="PLE93_02555"
     CDS             3214..3504
                     /locus_tag="PLE93_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85960.1"
                     /translation="MIGYEPGMNRQRLTGLTVTLLATALMALGLAACGSDDDGSGSSG
                     TTGTEVSQQMNGKKNEVTPDSKAPASAGNKDKNAPDDAISNRPGGPNKPASP"
     gene            complement(3501..3644)
                     /gene="nadA"
                     /locus_tag="PLE93_02560"
     CDS             complement(3501..3644)
                     /gene="nadA"
                     /locus_tag="PLE93_02560"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNH85961.1"
                     /translation="MAFCKYMKMITLPKLRDSLRDMKFEVKVDEALAEKARLPIERMV
                     SIG"
BASE COUNT          604 a         1193 c         1212 g          647 t
ORIGIN      
        1 gagcatgccg ccgatgacgg cccagacgcc aacggttgcg gtcttcaact ggccgccggc
       61 tgactctgat tgacgcccgg tagcgggccc ggccggctca gaccttcagg aatctgcgca
      121 tggtcagtcc ggacccgatc gccgaaacca ggatcgcggc gatgaacagg cagccgacga
      181 gggccgcaaa ggacatcgag tcctgtgcgt tcatcagggc gaacttgtcc gagagggggt
      241 cgacgactgt gaccttgcca atccacagca ccagtatcgc aatcgcgccg ccgaggatgc
      301 cgaccacgac cccttcgatc atgaagggcc agcggatgaa ccagcgggtc gcaccgacca
      361 ggcgcatgac ctcgatctcg cgccggcggg tgtagatgga aagccggatc gtgttgccaa
      421 tcaggagcag cgaggcacag atcagcagca cggtgatcac ggtgaggatc agtttcaggg
      481 cgccggtgac ctgttcgatg tcgcgggcgt cttcctggcg gtcatcgacc gaggcgatga
      541 tcggcgagat gtactggggg tcacccttgg cgccgaccgg ctggatcgcg ttgcggatcc
      601 cttccaggtt ggaagcgtca tcgggcttga tcacgaagtt ggccggcagc gggttcttgc
      661 caccgagctg cttcagggcg tcggggttct gcttgccgaa gtcactggcc aggaattcct
      721 tcttggcgtc ggccttggtc agcagggtta ccgactccac gtgtggcagc ccttccagct
      781 tcaccatcag ggcctgggtc tcggccttgg tcgcgtcgtc gtaaaggaag gtgcgcagct
      841 cgagctggcc acgcacgtcc tcgcccttgc tctgggcggt ctggaagacg gggatcagca
      901 caccgagcag aagcgcggtg agcacggtgg tcactaccgc agccgtggtc ggcgcgacgt
      961 tccgcttgat cgcgcggagc gcttcgccgg tgaaaaagaa gatcttgccc atcagtgtgc
     1021 tcccttgccc gaatttccag agttgccgtt ggtatggcct tcgacgccaa cgcccatctc
     1081 ggcccgcatg cggacaccga attcggtggt cgactcctcg gtgtaaccgc cagcgacctc
     1141 gtcgcggatg acccggcctt cgtagagctc gaccacgcgg cggcgcatgc ggtcgaccat
     1201 ttcccggtcg tgggtgacca cgacgacggt ggttccggtc ttgttgatcc ggtagagcag
     1261 ctgcatgatg ccgaccgagg tgaccgggtc gaggttgccg ctcggctcat cggccagaag
     1321 cagaggcgga tggttgacga aggcccgggc gaccgagacg cgctgctgct cgccgccgga
     1381 aagctcgtcg gggtagttgt gaagcttggt cgagagaccg accaggcgca gggtctcagg
     1441 caccttctgc cggatctcgg cccttgaagc gccgatcacc tgaagcgcgt aggcgacgtt
     1501 gtcgtaaacg gtgcggctcg gcagcagctt gaagtcctgg aagacggtgc cgatgttgcg
     1561 gcggagctgc ggaatcttct tctcggaaag ctggttgatg tcgcgaccgg cgatcgagat
     1621 cgtgccgtcg gtgggctcaa gctcgcgaat cagcatcttg atcagggtcg acttgccgca
     1681 gccggtcggg ccgaccagga agacgaactc gccacggttg atcgccagcg agacccggtc
     1741 aacggcaagg tggccgcccg ggtacacctt gctgacgtca cgcatctcga tgatcggggc
     1801 gccacccttg cgcggacgct cggcctgctt gcgggcgcgt gccgcttcga gctcggcgcc
     1861 tgctgattct cttttcttct ttccgaaagc catctggttc gccctattcc ccccggcccg
     1921 gggtaatcct tcccctgacg gggatctccc acaaagggca accccggcca ggttcatttc
     1981 ttcagagtta gcgaaacgtt agcaatcgct tatatgactt tggctgatct taagcgcggc
     2041 cggtaggatt cctgcaatgt ccagggctgc ccatttggct aaccgggccg ccctcgcagg
     2101 ttgcggcctc ctcctcgcga tcctgctcgc ggcgttcctt ctgccctcct ccccggccgc
     2161 ggccggcggc gaaccttcaa gcagtgaatc cggcgccacg gcctcgatcg tcaacggcaa
     2221 ggcaaccacg atcggccagt ggccctggca ggtcgctctg accgtgagct cgcggattgc
     2281 gccgcgggcc tcgaccagct ggcgtttctt ttgcggcggc tcggttctcg cgccgcggct
     2341 tgtgatcact gccggtcact gcgtcgccga cctcaaccgg gggcagatcc gcaagatcga
     2401 gatcgtttcc ggccgcaccc gtctcaattc gaaccggggc caggtggcca gggtgaccgg
     2461 gctccgcatg ccggtgaacg cctccggaaa gcggcgcttc cgcggtcttg ccggcgcggc
     2521 cgactgggac gtcgccctgc tgaccctcgc ttcccccctt tcggccgagc cgatcaagct
     2581 ggccggtccg gacgaggcgg cggcctggtc cccgggacag gtcgcctgga ccacaggctg
     2641 gggaatcacc aaggccttct cgcagcgggt tccggcgagg ctccaggtcg cccggcaggt
     2701 cctgatgggc aacggcctct gccaccgggc agacggcgtc gcgtacaagt cccgccggat
     2761 gatctgcatg ggagcacccc gggcccatgc ctcggcctgc aacggagaca gtggcggacc
     2821 tctcgtggtc gagaccggtg ccggttaccg gctggtcggc ctgaccagtt atggcgatgg
     2881 tgcctgccgc ggatttgtcc cctcggttga cacaagggtc tccggcaggc agatccgcaa
     2941 ctgggtcgca cggaccgcga tgaacctgac cggccgcaac gtggtcggca gcggtggcac
     3001 tcccaggccc gctccggact ggtgcaaggt gccggatgtc ttccggctca ggcagagcca
     3061 ggcgaaggcc aggctcgagg ccgcgggttg ccgcctcggc cgggtcagga ccgactatta
     3121 cagcgtcggc caggccggcc ggatcgtcgg cagccgcctc gcacccggat ggctggcccc
     3181 gcccggcttc aaggtgggag tttgggttgc cccatgatcg gctatgaacc gggcatgaac
     3241 cgccaacggc tgaccggatt gaccgtgacc ctcctcgcaa cggccttgat ggcccttggc
     3301 ctggccgcct gcggctccga cgatgacggc agcggaagct ccggcacgac cgggacggaa
     3361 gtgagccagc agatgaacgg gaagaagaac gaagtgacgc cggacagcaa ggctccggcc
     3421 agcgccggga acaaggacaa gaacgccccg gacgacgcta tctcgaaccg ccccggcggc
     3481 ccgaacaagc ccgcaagccc ttagccgata ctgaccatgc gctcgatcgg caggcgcgcc
     3541 ttttcggcca gcgcctcgtc gaccttgact tcgaacttca tgtcgcgcag cgagtcgcgc
     3601 agcttcggca gcgtgatcat cttcatgtac ttgcagaagg ccatgcggtt ggcctc
//
LOCUS       DAOQFI010000094         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11739_length_3652_cov_3.617737, whole genome shotgun sequence.
ACCESSION   DAOQFI010000094 DAOQFI010000000
VERSION     DAOQFI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11739_length_3652_cov_3.617737"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1587
                     /gene="rpsA"
                     /locus_tag="PLE93_02565"
     CDS             55..1587
                     /gene="rpsA"
                     /locus_tag="PLE93_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNH85962.1"
                     /translation="MTTTETTLQPAPEGVPVEGSNGLLINVDGRIIPNYAATIVSFEE
                     GDVVNGKVVRIDRDEVLLDIGYKSEGVIPAGELSIRKNVDTSEEVELGEEIDALVVTK
                     EDSEGRLILSKKRARFEKAWRKIEAAAENGEPVEGNVIEVVKGGLILDLGIRGFLPAS
                     LVDIRRVHNLDEFMSTTLECKVIELNRSRNNVVLSRRAVLEEERKEVRDQILDRLQPG
                     EVVEGKISNIVDFGAFVDLEGIDGLIHISELSWSHVNHPSEVVAIGDTVKIKVLDIDR
                     DRQRISLGLKQTQEDPWQRVVSTYRSGDVLEGTVTKVVSFGAFVEILDGVEGLVHISE
                     LADHHVENPSEVVELGASLNVKILEIDEERRRLSLSIKRVEGQNMLLKDLGAALAAAS
                     GEPAVEEETPELGLSEEVFADDAEAPADSAEEVAPEVEAVVDEAIAEAEVADAAEQAE
                     AADETPEEVVEDAVAEAEIEAAADEAAAEEAVVEEAGEITEEAAAEEAAEGEAPEGEE
                     QS"
     gene            1596..2180
                     /gene="coaE"
                     /locus_tag="PLE93_02570"
     CDS             1596..2180
                     /gene="coaE"
                     /locus_tag="PLE93_02570"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNH85963.1"
                     /translation="MTGGVAAGKSEALSAFGRLGAATISADQVVHDLLDREPLLGRLK
                     ERWGDEVAPEGRVDRGVVGAKVFNDPEELAWLESQIHPLVGEEFRGWIEGLDPATKFA
                     VAEIPLLFEGEMYRAFDHTVAIVAHEDTRQERARSRGHEGVEGREARQLSQAEKAERA
                     DTVIENDGTPAELESKLATLLEKFESELATGGQS"
     gene            2199..2843
                     /locus_tag="PLE93_02575"
     CDS             2199..2843
                     /locus_tag="PLE93_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNH85964.1"
                     /translation="MAQNRTSTAGGRRRSNPAPRRGSTKPRTGAGRAFLILAGLIAVG
                     ILAILIISGLASKGIKEVALPLKHEDIIRQQSQEKGVDASLIAAVIYAESQFVDQTSH
                     ADARGLMQITPATAEEIEKLSGGTTFKLEDLRDPELNIRYGTFYLAHLLEMYDGDVVA
                     ALAAYNGGPGNANEWGGADMTIDDITFPETREYVKKVLEKQREYKHEYSLELGY"
     gene            2843..>3652
                     /locus_tag="PLE93_02580"
     CDS             2843..>3652
                     /locus_tag="PLE93_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH85965.1"
                     /translation="MNLRTAALAGAVGLVLADSSIVVLALPEIFRQFDTSISGVSWVL
                     IIFNLVLALLAVPAAHMARRFGPGRSAAVGLAVFAGGSLVCGLATGLGPLLTGRAVQA
                     MGGAVAVVAALELMVSTFGDDRRAIATWVGAGAIGAAIGPGLGGLLTELVSWQSIFLV
                     QVPVAIVAGVPLKDIVIQETREWKIPDPVQPVEGNRPHLPANLALALVSASIAATLFL
                     IVLMLIEGWLLTPIEAALVVTVLPVAALVGSRIGNGIESERIRAASGAILLA"
BASE COUNT          676 a         1188 c         1199 g          589 t
ORIGIN      
        1 agagggcggg tcggaccccg cccgactcct gatatctgcc atcatccccg gactgtgact
       61 actactgaaa ccacactcca acccgcccca gagggggttc cggtcgaagg ctcaaacggc
      121 ctgctgatca acgttgacgg ccggatcatt cctaactacg cagcgacgat cgtttcgttc
      181 gaggaaggcg acgtcgtaaa cggcaaagtc gtccgcatcg atcgcgacga agtcctgctc
      241 gacatcggct acaagtcgga aggtgtaatc ccggccggcg agctctcgat tcgcaagaac
      301 gtcgacacct cggaagaggt cgaactcggc gaagagatcg acgccctggt cgtcaccaag
      361 gaagattccg aaggccggtt aatcctttcc aagaagcgtg cccgcttcga gaaggcctgg
      421 cgcaagatcg aagctgccgc cgaaaacggc gagccggtcg aaggcaacgt gatcgaggtc
      481 gtcaagggcg gcctgatcct cgacctcggc atccgcggct tcctgcctgc ctcgctggtt
      541 gacatccgcc gggtccacaa cctcgacgag ttcatgtcga ccacgctcga gtgcaaggtc
      601 atcgagctca accgcagccg caacaacgtc gtgctttccc gccgcgcagt cctcgaagag
      661 gagcgcaagg aagtccgcga ccagatcctc gatcgcctgc agccgggcga agtcgtcgaa
      721 ggcaagatct cgaacatcgt cgacttcggc gctttcgtgg acctcgaagg catcgacggc
      781 ctgattcaca tctccgagct ttcctggagc cacgtcaatc acccctccga ggtggtggcg
      841 atcggcgaca cggtcaagat caaggtgctc gacatcgacc gcgaccgcca gcgcatctcg
      901 ctgggtctca agcagaccca ggaggacccg tggcagcggg tcgtctcgac ctaccgttcg
      961 ggcgacgtgc tcgagggcac ggtcaccaag gtcgtctcct tcggtgcttt cgtcgagatc
     1021 ctcgacggcg tcgaaggcct ggtccacatt tcggaactgg ccgaccatca cgtcgagaac
     1081 ccgtcggaag tcgtcgagct cggcgccagc ctcaacgtca agatcctcga gatcgacgaa
     1141 gagcgtcgcc gcctctcgct ttcgatcaag cgggtcgagg gccagaacat gctgctcaag
     1201 gacctgggcg ccgccctggc cgccgcctcc ggcgagccgg ccgtcgagga agaaaccccg
     1261 gaactgggtc tctcggaaga ggtctttgcc gatgacgcgg aagccccggc cgatagcgcc
     1321 gaagaggtcg ctcccgaggt cgaggccgta gtcgacgagg cgatcgccga ggccgaagtg
     1381 gccgacgccg ccgagcaggc cgaggccgcg gacgaaaccc cggaagaggt cgtcgaggac
     1441 gccgtcgccg aggccgagat cgaagctgcc gctgacgaag ccgctgccga ggaggccgtg
     1501 gtcgaggagg ccggtgagat caccgaagaa gccgccgcgg aagaggcagc cgaaggtgaa
     1561 gctcccgaag gggaagaaca gtcctgacga tcggactgac cgggggcgta gctgccggta
     1621 agtccgaggc actgagcgcc tttgggcggc tcggcgcggc cacaatctct gccgatcagg
     1681 tcgttcacga cctgcttgac cgggagccgc tgctgggtcg cctcaaagag cgctggggag
     1741 acgaggttgc cccggaaggc cgcgtggatc gcggggtggt cggggcaaag gtcttcaacg
     1801 acccggaaga gctggcctgg ctcgaatcgc agatccatcc gctggtcggc gaggaattcc
     1861 gcggctggat cgagggtctc gatccagcga cgaagttcgc ggtggccgaa atcccgctcc
     1921 tcttcgaggg tgagatgtac cgcgccttcg atcacaccgt cgcgatcgtg gcgcatgaag
     1981 acacccgcca ggaacgggcc cggtcgcgcg ggcatgaagg ggtcgagggc cgggaggccc
     2041 ggcagctgag ccaggctgaa aaggccgagc gggcagacac ggtcatcgaa aatgacggaa
     2101 ccccggccga actcgaatcg aagctggcga ccctgctcga aaagttcgag tcagagttgg
     2161 cgaccggcgg gcaaagctag gctataaggc cacctcgcat ggcccagaac cgaacttcaa
     2221 cagcgggcgg tcgacgccgc tcaaacccgg ccccccggcg cggcagcacc aaaccgcgca
     2281 ccggcgcagg ccgggccttc ctgatcctgg ccggcctgat cgcggtcggc atcctcgcga
     2341 tcctgatcat ctccgggctg gccagcaagg gaatcaagga agtggcgttg cccctgaagc
     2401 acgaggacat aatccggcag cagtcgcagg aaaaaggtgt tgatgcctcg ctgatcgccg
     2461 cggtgatcta cgccgaatcc cagttcgtcg accagacttc ccacgccgac gcgaggggcc
     2521 tgatgcagat cacgccggcc accgcggagg agatcgaaaa gctgagcggt ggcaccacct
     2581 tcaaactcga ggatctgcgc gatccggagc tgaacatccg ctacggcacc ttctacctcg
     2641 ctcacctgct ggagatgtac gacggtgacg tggtcgcggc ccttgctgcc tacaacggcg
     2701 ggccgggcaa cgccaacgaa tggggcggag ccgacatgac gatcgatgac atcaccttcc
     2761 ccgaaaccag ggaatacgtg aagaaagtcc tcgagaagca gcgggagtac aaacacgagt
     2821 attccctgga gctcgggtac tgatgaacct gcgcacggcc gcgctggcgg gtgcggtcgg
     2881 cctggttctc gccgactctt cgatcgtcgt actcgccctg ccggagatct tccgccagtt
     2941 cgacaccagc atctccggtg tctcctgggt gctgatcatc ttcaacctcg tcctggccct
     3001 gctcgccgtg cctgccgccc acatggcccg ccggttcggt cccggccgct cggccgcggt
     3061 cggcctggcc gtcttcgccg gcggcagcct ggtctgcggg ctggcgaccg gcctcgggcc
     3121 gctcctgacc ggtcgcgcgg tccaggccat gggcggtgcc gtcgcggtgg tcgccgcgct
     3181 cgagttgatg gtttccactt tcggtgacga tcgccgggcg attgccacct gggttggcgc
     3241 cggtgcgatc ggtgccgcga tcggacccgg gctcggtggc cttctgaccg agctcgtttc
     3301 ctggcagtcg atcttcctgg tccaggtgcc ggtggcgatc gtcgccggcg tgccgctcaa
     3361 ggacatcgtg atccaggaaa cccgtgagtg gaagatcccg gacccggtcc agccggtcga
     3421 gggcaaccgc ccgcatctgc cggcaaacct ggccctggcc ctggtctccg cctcgatcgc
     3481 ggcgacgctc ttcctgatcg tcctgatgct gatcgagggc tggctgctga ctccgatcga
     3541 agcggctctg gtcgtgacgg tgctgccggt agctgccctg gtcgggtcga gaatcggcaa
     3601 cgggatcgaa tcggaacgga tccgcgccgc ttccggggcg atcttgctgg cc
//
LOCUS       DAOQFI010000095         3630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11864_length_3630_cov_3.107972, whole genome shotgun sequence.
ACCESSION   DAOQFI010000095 DAOQFI010000000
VERSION     DAOQFI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11864_length_3630_cov_3.107972"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1855)
                     /locus_tag="PLE93_02585"
     CDS             complement(179..1855)
                     /locus_tag="PLE93_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH85966.1"
                     /translation="MGVLILFRNNRWRFRVLWLLTGLAAALLTAAFGHSVGLARARPN
                     LVVIQTDDQTVRMLHSHFVGPHGKKHRTMPHVQRLIEDRGLEFTNYYASIPVCSPSRS
                     ALLSGQYGHTSGLIRNVGKLGGVRGISRSPVWKRNLAVALRRVGYYTAHFGRFVNQYG
                     NPEGVDRRAVPPGWSWWATDWTPDVARRFYGYKLNVNGHIQGPFGKLHYQAWSNKDSR
                     KCPAGGLSCNYHTDQITTRALRVIRHAPRRPIYIQVDYEAPHATADGSGESEPASRHI
                     GSARRTPLPKPPGFNERNIRDKPRVLRSRARRLRAGQIAGMRTRWQRELESLRAVDES
                     VGRIVRTLRDTGRLARTYIFFIGDNGAFFGEHRYARSKFLAYEPSSNVPMLVRGPGVK
                     RGRRSGAIVSNVDLAPTIADLAGARLLLKPDGRSMKRLFRFPRRVGRRAVILESYRLP
                     SRPLYQHLEGEGIDTPHDLGPSASAVVPAVNYTAIRAGRYKYIDYEGGGRELYDLKLD
                     PYEVSNRVHWRGYRKVVRKLARQLKWRRFCSGAKCRRGTGRLPLPKLRRH"
     gene            1915..>3630
                     /locus_tag="PLE93_02590"
     CDS             1915..>3630
                     /locus_tag="PLE93_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH85967.1"
                     /translation="MSDREEKAAPLDPPSILMSGLHLAALWALAIVQPLLNLLGNNPD
                     FFVARDNTSSQIIIFVLLLTILPPLFAGLIEALVNLLSQAARWIVHLAFCAVLFWAIA
                     LQVLKQFADGPAWPMIIFAIVLGGLLTWAYAKARFMRSMTDILIIAPPVILIAFFFFS
                     DAKELTLSTTEVDAKTVNASNPHPVVMVIFDEFPAGSLMTPSGRINPKRFPNFAGLKG
                     TSTWYRNTATDASYTAIAVPSILTGQPADHDSLPTAADHPDSIFTLLGGSWQTHAIEP
                     ITQLCTEEICGEREGGKTGMADALSSLTTDLKAVSAHLLLPADMGSSLPDISQSFQGF
                     GGEEDSVERGRARQWVHDRLGEGENSLDGEADVDAFIRRLGSSGKSFDFVHVEKPHYP
                     WTHYPSGLKYSDGTEDFRSFIGETDWEGDGYITDRAHQAHLLEVGFTDNLLGRIISAL
                     KRDGRWDDTLFVATADHGAAWTKGAGRRSAEEETMGQIGMVPLFIKAPGQEQGKVVDR
                     PTCTTEIVPRMAQILGIDLPWTPATCDRKTVRIDNGSGKPVTLPFVKAIAQRQLYINR
                     MARLLG"
BASE COUNT          630 a         1228 c         1166 g          606 t
ORIGIN      
        1 atcggtctgg atcacgacga aactaggccg gcccggatca agggcggccc ggggaatcga
       61 agccgcgctg gcgcggccca tccccaccgc cagaagcagt gcgaagagca gcgtgaatgc
      121 tgccgaccgg gtgaagatgt tgcgatttgc tcccctgatg tctgccccct gaaaccggtc
      181 agtggcgccg gagtttcggc aatggaagcc gcccggttcc gcgcctgcat tttgccccgg
      241 agcagaacct acgccatttc aactgccggg ccagtttccg gaccaccttc cggtacccgc
      301 gccagtggac acgattggaa acctcgtacg ggtcgagctt gaggtcatag agctcgcgcc
      361 cgccgccttc gtaatcgatg tacttgtaac ggccggcgcg aatcgccgtg tagttgaccg
      421 cggggaccac ggccgaggcc gagggaccaa ggtcatgggg cgtgtcgatc ccttcgcctt
      481 ccagatgttg gtagagcggt ctcgaaggca gccggtagga ctcgaggatc acggcccgcc
      541 gtccgacccg ccgggggaac cggaacagcc gcttcattga ccttccgtcc ggcttcagca
      601 gcagccgggc gccggcgaga tcggcgatgg ttggcgcgag atctacattg ctgacgatcg
      661 cgcccgagcg acggcctcgc ttgaccccgg gaccccgaac cagcatcggc acgttgctcg
      721 aaggctcgta ggcgaggaac ttggaccggg cgtagcggtg ctcgccgaag aaggcgccgt
      781 tgtcaccgat gaagaagatg taggtgcggg ccaggcgacc ggtgtcccga agggtcctga
      841 ctatccttcc gacgctctcg tccacggccc ggagcgactc gagctcacgc tgccagcggg
      901 tccgcatccc ggcaatctgg ccggccctca ggcgccgggc ccgggaacgc aggacccggg
      961 gcttgtcgcg aatgttccgc tcgttgaagc ccggaggctt cggcagcggc gtgcggcggg
     1021 ccgaaccgat gtgacggctg gcgggctccg actcgccact gccgtcggcg gtcgcatgcg
     1081 gggcctcgta atcgacctgg atatagatcg gccgccgcgg cgcgtgccgg atcacccgca
     1141 gggcccgggt cgtgatctga tccgtgtggt agttgcagct cagcccgccg gccgggcatt
     1201 tgcggctgtc cttgttgctc caggcctggt agtggagctt tccgaacggc ccctggatgt
     1261 gaccgttcac gttcagcttg tacccgtaga accggcgggc gacatccgga gtccagtcgg
     1321 tcgcccacca ggaccatccc ggcgggacgg cacgccggtc gacgccctcc gggttgccgt
     1381 actggttcac gaaccggccg aaatgcgcgg tgtagtaacc gacccggcgc agggcgacgg
     1441 ccaggttccg cttccagacc gggcttcgcg aaatgcccct taccccgccc agcttgccga
     1501 cgttgcggat cagcccgctg gtgtgcccgt actgcccgga aagcagggcc gaacgggaag
     1561 gcgaacagac cggaatcgac gcgtagtagt tcgtgaactc aaggccgcgg tcctcgatca
     1621 ggcgctggac atgcggcatc gtgcggtgct tcttgccatg cggaccgacg aagtgcgagt
     1681 ggagcatccg cacggtctgg tcgtcggtct ggatgaccac caagttggga cgggcccggg
     1741 caagcccgac cgaatggccg aacgcagcgg ttagcagtgc cgcagccagg ccggtcagca
     1801 gccagaggac gcgaaaacgc cagcggttgt tcctgaagag gatcaagacg cccatcggtt
     1861 gaaacccgct tcatagcggc gagttcccct tcaagagaat aggatcgccg accgatgtct
     1921 gaccgggagg aaaaggccgc acccctggat ccgccctcga tcctgatgag cggcctccac
     1981 ctcgccgccc tctgggccct ggcgatcgtc cagccgttgc tgaacctgct gggcaacaac
     2041 cccgacttct tcgtcgcccg cgacaacacc agcagccaga tcatcatctt cgtcctgctg
     2101 ctgacgatcc tgccgccact tttcgcgggg ctgatcgagg ccctggtcaa tctgctcagc
     2161 caggcggccc ggtggatcgt tcacctcgcc ttctgtgccg tgctcttctg ggcgatcgcc
     2221 ctgcaggtgc tgaagcagtt cgcggacggt cctgcctggc cgatgatcat cttcgcgatc
     2281 gtgctcggcg gccttttgac ctgggcctac gccaaggccc gcttcatgcg ctcgatgacc
     2341 gacatcctga tcatcgcccc gccggtgatc ctgatcgcct tctttttctt cagcgacgcc
     2401 aaagagctca cgctctcgac cacggaggtt gacgccaaga cggtgaacgc ctcgaacccg
     2461 catccggtcg tgatggtcat cttcgacgag ttcccggccg gctcgctgat gaccccttcg
     2521 ggccggatca acccgaagcg cttccccaac ttcgccggcc tgaagggcac ctctacctgg
     2581 taccggaaca cggcgaccga cgcttcgtac acggcgatcg cggtgccctc gatcctgacc
     2641 ggccagcccg cggatcacga cagcctgccg accgcggccg accaccccga ttcgatcttc
     2701 accctgctgg gcgggagctg gcagacccac gcgatcgaac cgatcaccca gctctgcacc
     2761 gaggagatct gcggcgagcg ggaaggcggg aagaccggca tggccgatgc gctctcctcc
     2821 ctgaccactg acctgaaagc ggtttccgcc catctgctgc tgccggccga catgggttcc
     2881 tccctgccgg acatttccca gagcttccag ggtttcggtg gcgaggagga ctcggtagag
     2941 cggggccggg cccgccagtg ggtgcatgac cgcctcggcg agggcgagaa ctcgctggat
     3001 ggtgaagctg acgttgacgc gttcatccgc cggctgggaa gcagcggcaa gagcttcgac
     3061 ttcgttcacg tcgagaagcc gcactatccc tggacccact acccctcggg gctgaagtac
     3121 tccgacggga ccgaggactt ccgcagcttc atcggtgaaa ccgactggga gggtgacggc
     3181 tacatcacgg accgggccca tcaggcccat ctgctcgagg tcggcttcac cgacaacctg
     3241 ctcggccgga tcatttccgc gctgaagagg gacggccgct gggatgacac gctgttcgtt
     3301 gccacggccg atcacggtgc agcctggacc aaaggtgccg gccgacgctc ggcggaggaa
     3361 gaaacgatgg ggcagatcgg gatggtcccg ctcttcatca aggcacccgg ccaggagcag
     3421 ggcaaggtag tcgaccgacc gacctgcacc acggaaatcg tcccccggat ggcgcagatc
     3481 ctcggcatcg acctgccctg gacgccggcc acctgcgacc gcaaaaccgt ccggatcgac
     3541 aacggaagcg gcaagccggt gaccctcccc ttcgtcaagg cgatcgccca gcgccagctc
     3601 tacatcaacc ggatggcccg gctgctcggc
//
LOCUS       DAOQFI010000096         3630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11866_length_3630_cov_2.618741, whole genome shotgun sequence.
ACCESSION   DAOQFI010000096 DAOQFI010000000
VERSION     DAOQFI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11866_length_3630_cov_2.618741"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1057
                     /locus_tag="PLE93_02595"
     CDS             125..1057
                     /locus_tag="PLE93_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNH85968.1"
                     /translation="MSARWGLTLPLPGVSLADTKDFVQLAEAEGYTDLWTGETGGPDG
                     FTPLALSAAWTSKVRLGTGIVGVFQRGPALLAQQAAALTDASSGRFALGIGASSDRMV
                     EGWNQIPFVKPLSKVEQTVDFLRVALAGERTDTGFKLETAPTHETPIVLAALRGKMLE
                     LAVNKADGAFTNFLPLAGLPKVVDGIEGAGNDFELLCRFFCIPGEKEQVEGLARFMFS
                     SYITVPVYANFFRWLGYGEEIEAMVEHWNNKDRQAAAAAAPWELIEDTFLLGSPEQIR
                     ARLDEYVAGGITLPVITPVTTPDNHAAMIKALAP"
     gene            complement(1155..2057)
                     /locus_tag="PLE93_02600"
     CDS             complement(1155..2057)
                     /locus_tag="PLE93_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85969.1"
                     /translation="MGVRLKSTRGQSTIEWVGLITLVAALMAALTAAGIQVPAVGLAH
                     SVSTRLLCAASLSGSCAGEGSLDEAYGPEVAELVRSGAPALMYGKDMLGLPVDYRTCR
                     SPWCADGTGEGEVSRSTAGEPVTLFTRVIHEPDGSIYIQYWAYYPESASLRGAPLLED
                     EGYHRNDWESMQIRVDPDGVVSERASSHAGYNHTRSSVNWGSDLGWGFLTDAAEKVGL
                     REKGGWGDATGQYLIAGGSHAGNVAASLADSDYPSYTPSALVRLVPLESVKDGPLARP
                     ADFDPITPPWEKEVWTEPEAEGTG"
     gene            2141..2419
                     /locus_tag="PLE93_02605"
     CDS             2141..2419
                     /locus_tag="PLE93_02605"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNH85970.1"
                     /translation="MADRNKMTPPSSASKKTPEWKWWAFGIAVLLLLIVVLQNSQDVE
                     FKILFLVDTAAPLFVLLLFAAAIGAIIGYTGPILRRHRHNTRKEYGKD"
     gene            2440..3042
                     /locus_tag="PLE93_02610"
     CDS             2440..3042
                     /locus_tag="PLE93_02610"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNH85971.1"
                     /translation="MIEPVRLEGDRVALEPIRADLADELWIAAQDPEIWHWTTHLNHS
                     REFFDGWVAGGIEGWESGERSTFVTSLRSAGSDEPARVVGSSSYLFYRPDEDVVEIGS
                     TWLNPGAWGTGANTEAKLLMMTHAFETLGCVRVEFKTDRRNERSRAALAALPAQFEGI
                     LRKHTNPFGVGRRDSAYYSVIDDEWPEVRAALEARLARRS"
     gene            complement(3065..>3630)
                     /locus_tag="PLE93_02615"
     CDS             complement(3065..>3630)
                     /locus_tag="PLE93_02615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNH85972.1"
                     /translation="RLEQIHRLLNETGTLYLHIDYREAHYVKVMLDGIFGRESFLNEI
                     IWAYDYGGKSKRRWPAKHDTILVYVKDPDRYYFDSGEVDREPYMAPGLVTPEKVAKGK
                     LPTDVWWHTIVPTNGAEKTGYPTQKPEGIVRRMVLASTRPGDLCLDPFCGSGTLGAVC
                     RKLGRDYLLIDENPEAVEIAGKRIGQD"
BASE COUNT          606 a         1250 c         1164 g          610 t
ORIGIN      
        1 cggcgagggc cgccgactcg gtgacgcgaa ccagctccag ggccaggttg cggtcgggct
       61 ttgatccgga tcgaacgtcg tgcgagataa cacccatgcg gggcaggcta tataggctgc
      121 cgccatgagc gcacgctggg gcttgaccct ccccctgccc ggggtctcac tggccgacac
      181 caaggacttc gttcagctgg cggaggccga gggttacacc gacctctgga ccggggaaac
      241 cggcggcccg gacggcttca cgccgctcgc cctgagtgcc gcctggacca gcaaggtgcg
      301 gctcggcacc ggcatcgtcg gggtcttcca gcgcggaccg gcgctgctcg cccagcaggc
      361 cgccgccctg accgacgctt cctccggccg cttcgccctc ggcatcggcg cctcctccga
      421 ccgcatggtc gagggctgga accagatccc cttcgtcaag ccgctttcca aggtcgaaca
      481 aaccgtggac ttcctgcggg tagccctcgc cggggagcgc accgacaccg gcttcaaact
      541 ggagacggcc ccgacccacg aaaccccgat cgtgctggcc gccctgcgcg gcaagatgct
      601 cgagctggcg gtcaacaagg ccgacggcgc cttcaccaac ttcctgcccc tggccggcct
      661 gccgaaggtg gtcgacggca tcgagggcgc cggcaacgac ttcgagctgc tttgccgttt
      721 cttctgcatc cccggtgaaa aggaacaggt cgagggcctc gcccggttca tgttctccag
      781 ctacatcacg gtgccggttt acgcgaactt cttccgctgg ctcggctacg gcgaagagat
      841 cgaagcgatg gtcgagcact ggaacaacaa ggaccgccag gccgccgcgg cggccgcgcc
      901 ctgggagctg atcgaagaca ccttcctgct cggcagcccg gagcagatcc gcgcgaggct
      961 cgatgaatac gtcgccggcg ggatcaccct gcccgtgatc accccggtca ccaccccgga
     1021 caatcacgcc gcgatgatca aggccctggc tccctgaagc cttcctccaa tcgagaaggc
     1081 gaaagtggcc acatagcgct ccattcagcc ttctcggctg ctcccgacat gcgggcagct
     1141 cgaaagccag ccggctagcc ggttccctcg gcttcgggtt cggtccagac ctccttctcc
     1201 cagggcggcg tgatcggatc gaagtcggcc ggtcgtgcga gcggaccgtc cttgaccgat
     1261 tccagcggga ccagccggac cagggcggac ggagtgtagg aggggtagtc cgaatcggcg
     1321 aggctggcgg caacgttgcc ggcgtggctg ccgccggcga tcaggtactg cccggtcgca
     1381 tcaccccagc ctcccttctc gcgcaggcca accttctctg ccgcgtcggt cagaaagccc
     1441 cagccgaggt cggagcccca gttgaccgac gaccgcgtgt ggttgtagcc ggcatgggag
     1501 gaagcccgct cgctgaccac tccgtcggga tcgaccctga tctgcattga ttcccagtca
     1561 ttccggtggt agccctcgtc ctcgaggagc ggtgcccctc gaagcgaggc gctctcgggg
     1621 tagtaggccc agtactggat gtagatgctg ccgtcgggct cgtggatgac cctggtgaaa
     1681 agcgttaccg gctctccggc agtggaccgg ctgacctcgc cctccccggt tccgtcggcg
     1741 caccagggcg atcggcaggt cctgtaatcg accggcaggc cgagcatgtc ctttccgtac
     1801 ataagggccg gcgccccgga acgaacgagt tcagcgactt ccggaccgta ggcctcgtcg
     1861 agcgaaccct cgccagcgca ggaaccggac aacgaggcgg cgcagagcaa cctggtcgag
     1921 acggaatggg caagcccgac cgccggcacc tggattcccg ccgcggtgag agccgccatc
     1981 agggccgcga ccagggtgat caacccgacc cactcgatcg tcgattggcc ccgcgtcgat
     2041 ttcagcctca cgcccatcgc ccaatcgtgg cgggaaaaag ctcacaagtg tgttaccggt
     2101 gcaacgattc agtgacggac ggccgaggta aaatcggtcc atggccgatc gcaacaagat
     2161 gaccccgccg tccagtgctt cgaagaagac ccccgaatgg aagtggtggg ccttcggcat
     2221 cgcagtcctc ctgctgctga tcgtggttct gcagaactcc caggacgtgg agttcaagat
     2281 tctcttcctt gtcgacaccg cggcgccgct cttcgtgctg ctgctgttcg ccgcggcgat
     2341 cggcgcgatc atcggctaca ccggcccgat cctccgccgc caccgccaca acacccgcaa
     2401 ggaatacggc aaggactgag cggcgcgatg tcgcttccga tgatcgaacc ggtccgcctc
     2461 gaaggcgacc gggtggcact ggagccgatc cgtgccgatc tcgccgacga gctctggatc
     2521 gccgcgcagg atcccgagat ctggcactgg accacccatc tgaaccacag tcgcgagttc
     2581 tttgacggct gggttgcggg cggcatcgaa ggctgggaga gcggcgagcg cagcaccttt
     2641 gtgaccagcc tccggtccgc gggaagcgac gagccagccc gggttgtcgg cagcagcagc
     2701 tacctcttct accggccgga cgaggacgtg gtcgagatcg gctcgacctg gctgaatccc
     2761 ggggcctggg gcacgggcgc caacaccgaa gcgaagctgc tcatgatgac ccacgctttc
     2821 gagacgctcg gctgcgttcg ggtcgagttc aagaccgacc gtcgcaacga gcgctcgcga
     2881 gcggcactcg cggctttgcc ggcgcagttc gaggggatcc tgcgcaagca cacgaatccg
     2941 ttcggggtcg gccgccgaga ttccgcctac tacagcgtga tcgatgacga gtggcccgaa
     3001 gttcgcgcgg cgctcgaggc ccgcctggcc cgccgatcct gagccccggc cccggggcgc
     3061 cgcttcagtc ctgcccgatc cgctttcccg cgatctccac ggcttccggg ttctcgtcga
     3121 tcagcaggta gtcccggccc agtttccggc agaccgcgcc gagcgttccc gaaccgcaga
     3181 aggggtcgag gcaaaggtcc cccggccggg ttgatgcgag caccatcctg cgtacgatcc
     3241 cttccggttt ctgggtcggg tagccggtct tctcggcccc gttggtcggc acgatcgtgt
     3301 gccaccagac gtcggtcggc agtttgccct tggccacctt ctcgggggtg accagccccg
     3361 gcgccatgta cggctcccgg tccacctcgc cggagtcgaa gtagtagcgg tccggatcct
     3421 tgacatagac caggatcgtg tcgtgcttgg ccggccagcg ccgcttcgac ttgccgccgt
     3481 agtcgtaggc ccagatgatc tcgttgagga acgactcccg gccgaagatg ccgtccagca
     3541 tcactttcac gtaatgggct tcgcggtagt cgatatgcag gtagagcgtg cctgtttcgt
     3601 tcagcaggcg gtggatctgc tccagccgcg
//
LOCUS       DAOQFI010000097         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11948_length_3616_cov_2.939343, whole genome shotgun sequence.
ACCESSION   DAOQFI010000097 DAOQFI010000000
VERSION     DAOQFI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11948_length_3616_cov_2.939343"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLE93_02620"
     CDS             <1..783
                     /locus_tag="PLE93_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNH85973.1"
                     /translation="GHVPTVPPPVNAWNHAGPLARRVEDLALALGVLDGSGEADLPGV
                     EGIRCRVFTGNRSMIVSPEVKSAVRRAAGALGSAGMEVEQSPALPIQRMTMLYSHVLR
                     KHALPEINAELGGGERFNWLAEVRRTLTGRARISAEALSVYAYIAYGGVLKPGPGDAD
                     RKLERLRRQVIETVGDGVLVCPLLLVRPARHGATWLPFTQIPMAVPFNITGLPVAMVP
                     VYWTANGLPLSVQVVAGPGKDELALAAARELEERLGGWKPID"
     gene            913..1662
                     /locus_tag="PLE93_02625"
     CDS             913..1662
                     /locus_tag="PLE93_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85974.1"
                     /translation="MNDGANWLADTATAVADRLGLPHNRLFDEWASTLDPSRWPEPPE
                     GDGRPAMLIPGFLAGDPSLSNMARWLKRGDWNMTRSGINWNVNCTQATIEGVEARLRA
                     SVEASGKRALLVGQSRGGAMGKVIAVRNPDLVETLVTLGSPLNDHFNIHPRTWFGIGI
                     IGGLGELGFPGFLSRDCATGDCCKETRIAYGRPFPKDVRFISFYSRADTIVRYQACLH
                     QAAEHVEIESSHLGMGLDSEVWVRMSEELSR"
     gene            1664..>3616
                     /locus_tag="PLE93_02630"
     CDS             1664..>3616
                     /locus_tag="PLE93_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNH85975.1"
                     /translation="MAELKQLTSLDAQFLAIETDRNYGHVGGLAILDPTTAPGGKLTI
                     EDLRQLIQDRLHLIAPFTQRLVSVPLSIDWPYWTQDEHFDIGYHCREIALPEPGSHEQ
                     LLEQVGRIYSRRLDRSRPLWEIYLIHGLEGGKVALLSKTHHAAIDGMSGAEILSALYD
                     LGPEPRKVDPPATAPDRSAPDGKDLLMMWVKGIPRSPLNLVDRLTTVVPHVDMAVAAF
                     GLPGSRRISRVMSRVRIRFGAEEEHVIERPPVKAPKGPYGAALSPHRVFGLGQVSLDD
                     VKTVKNAFGVKVNDVVICLVAGAVREHLTFTGELPEEPLLAMIPVSVRTEEERGTFGN
                     KVSAMIVPLPTHIDDPAERLEFIHSVMGAAKDSHDALPAEALRDLTDFVPPALHARAA
                     RVVTTVAGRFTRPPWNVIISNVPGPPIDLYFAGAKLEAMYPLSIISDGIGLNVTIMSY
                     RNSIDIGITADREQTPEVQTLVDGMQSELELLLAAARAAGIPSVSLDLIAGTPGETDV
                     DWANTVAAALAMHPDHLSAYSLGIEGGTRLAARVRRGDLVEPSPDEAASRYEHLDAAA
                     SSAGLSWYEVSNWARTTADRCRHNLGYWRDDDWWGIGPGAHSHIDGRRWWNHSGLDDW
                     STEALAGRVPAAGSETPTAEERLTEHI"
BASE COUNT          608 a         1222 c         1190 g          596 t
ORIGIN      
        1 ggccacgtgc cgaccgtgcc gccaccggtg aatgcctgga accatgcggg accactggcc
       61 cgccgggtcg aagacctcgc cctcgcgctg ggtgtgctcg acggcagcgg cgaagcagac
      121 ctgcccgggg tggaagggat ccgctgccgg gtcttcaccg gcaaccgctc gatgattgtc
      181 agccccgaag tgaaatccgc ggtccggcgt gcggctggcg cactcggatc ggccggaatg
      241 gaagtcgagc agagtccggc tttgccgatc cagaggatga ccatgctcta ctcgcatgtg
      301 ctcagaaagc acgcattgcc cgagatcaac gccgaactcg ggggaggcga gcggttcaac
      361 tggctcgcgg aagttcgacg caccctcacc ggccgggcaa ggatctcggc cgaggcactc
      421 tcggtctacg catacatcgc ctacggcggc gttctcaaac ccggtccagg tgatgccgac
      481 cggaagctcg agcggctgcg ccggcaggtg atcgagacgg tcggggacgg tgtgctcgtc
      541 tgtccgcttt tgctggtccg accggcccgg cacggggcga cctggctgcc attcacccag
      601 attccgatgg cggtcccttt caacatcacc gggttgccgg tggcgatggt gccggtgtac
      661 tggaccgcga acggtcttcc actttcggtt caggtggtgg ccggcccggg caaggacgaa
      721 ctggccctgg ctgccgcgag ggagctggag gagcggctcg gcggctggaa accgatcgac
      781 tgagccagtc tgcaacccgg cctccggggc gcaacacatg gctggtccgc tgcgccgggg
      841 agtaacattc atcacatgga attgccactg ggagggtttc catcaattgg aaaggttcca
      901 acgctgccgt ccgtgaacga cggcgcaaac tggctggctg acaccgccac cgcggtcgcc
      961 gaccggctgg gacttccgca caaccggctt tttgacgaat gggcatcgac cctcgatcca
     1021 tcccggtggc ccgaacctcc cgagggtgac ggccggccgg cgatgctgat ccccggtttc
     1081 ctcgccggtg acccgagcct ttcgaacatg gcccgctggc tgaaacgcgg cgactggaac
     1141 atgaccagga gcggcatcaa ctggaacgtc aactgcaccc aggccacgat cgaaggcgtc
     1201 gaggcgcgac tccgggcgtc ggtcgaagcc agcggcaaac gggccttgct ggtgggccag
     1261 agccggggcg gagcgatggg caaggtgatc gcggtccgca accccgacct ggtcgagacc
     1321 ctggtgaccc tgggctcccc gctgaacgat cacttcaaca ttcacccccg cacctggttc
     1381 gggatcggca tcatcggcgg actcggtgaa ctcggtttcc ccggtttcct cagccgtgac
     1441 tgtgccacgg gggactgctg caaggaaacc cggatcgcct acggccggcc gttccccaag
     1501 gatgtccgct tcatctcctt ttactcgcgc gccgacacca tcgtgcgtta ccaggcctgt
     1561 ctccatcagg ccgccgagca cgtcgagatc gagagcagcc acctcgggat gggcctcgac
     1621 tcggaagtct gggtccgcat gagcgaggaa ctcagccgct gagatggccg agctgaagca
     1681 gctcacctca cttgacgccc agtttctggc gatcgaaacc gatcggaact acggccacgt
     1741 cggcggcctc gcgattctcg accccaccac ggcccccggc ggcaagctga cgatcgaaga
     1801 tctgaggcag ctgattcagg accggctgca cctgatcgct ccgttcaccc agcggctggt
     1861 ctcggttccg ttgagcatcg actggccgta ctggacccag gatgaacact tcgacatcgg
     1921 ttaccactgc cgggagatcg cgctcccgga accgggcagt cacgagcagc tgctggagca
     1981 ggtcggccgc atctattcgc ggcgactcga tcgttcccgt ccactctggg agatctattt
     2041 gatccacggg ctcgagggcg gcaaggtcgc gctgctttcg aagacccatc acgcggcgat
     2101 cgacggcatg tccggcgccg aaatcctctc cgccctttac gacctcggtc ccgagccgcg
     2161 gaaggtggac ccgccggcga ccgctccgga ccggtccgct ccggacggca aggacctgct
     2221 gatgatgtgg gtgaagggga ttccccgctc gccgctcaac ctggttgacc ggctgaccac
     2281 cgtggtgccg cacgtggaca tggcggttgc ggccttcggc ctcccgggca gccggcggat
     2341 aagccgggtc atgtcccggg tgaggatccg cttcggagcc gaagaggagc atgtgatcga
     2401 aaggccgccg gtaaaggcgc cgaagggtcc ttatggcgcg gcactttcgc cgcaccgcgt
     2461 tttcggcctg ggacaggttt ccctcgatga cgtgaagacc gtcaagaacg cgttcggagt
     2521 caaggtcaac gacgtggtga tctgcctggt cgcgggggcg gtccgcgagc atctcacctt
     2581 cacgggcgag ctgccggagg aaccgctgct ggcgatgatc cccgtctccg tccggaccga
     2641 ggaagagcgc ggcaccttcg gcaacaaggt ctcggcgatg atcgtgccgc tgccaaccca
     2701 catcgatgat cccgccgagc ggctcgagtt cattcattcg gtcatgggtg ccgccaagga
     2761 cagtcacgat gcccttccgg ccgaggctct gcgtgacctc accgacttcg tgccaccggc
     2821 acttcacgcc cgggccgccc gggtcgtcac caccgtggcc gggcgcttca cccggccccc
     2881 ctggaacgtg atcatctcga atgtccccgg acccccgatc gacctctact tcgccggggc
     2941 gaagctcgag gccatgtatc cgctctcgat catttcggac gggatcgggt tgaacgtgac
     3001 gatcatgagc taccggaatt cgatcgacat cgggatcacc gccgaccgtg agcagacccc
     3061 cgaggtccag accctggtcg acggcatgca gtcggaactc gagctgctgc tggccgcagc
     3121 ccgcgccgcc ggaatcccga gcgtgtcgtt ggacctgatc gccgggacgc ccggcgagac
     3181 cgatgtggac tgggccaaca ccgtcgccgc ggcgttggcg atgcaccccg accacctgtc
     3241 ggcgtactcg ctcggcatcg aaggcggcac ccggctggcc gcccgtgtac gccgtggcga
     3301 tctggtcgag ccgtcaccgg acgaggcggc ttcgcgctac gagcacctcg acgcggcggc
     3361 ctcctccgct ggtctgagct ggtacgaggt gtccaactgg gcccggacca cggcggatcg
     3421 ctgtcgccac aacctcggct actggcggga cgacgactgg tggggcatcg gtcccggggc
     3481 ccactcacac atcgacgggc gccgttggtg gaaccactcc ggcctggacg actggagcac
     3541 cgaggcgctt gcgggccggg tgcccgccgc cggctccgag acgccgaccg cggaggagcg
     3601 gctgaccgag cacatc
//
LOCUS       DAOQFI010000098         3607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11993_length_3607_cov_2.768018, whole genome shotgun sequence.
ACCESSION   DAOQFI010000098 DAOQFI010000000
VERSION     DAOQFI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11993_length_3607_cov_2.768018"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1784)
                     /locus_tag="PLE93_02635"
     CDS             complement(60..1784)
                     /locus_tag="PLE93_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNH85976.1"
                     /translation="MKTAAKLLSLGLTVGVLATTATVPASAADTYLDCSAAIDGDGSP
                     AQPRNSLGSAFDLGPGDSLLLKRGTTCHGRLEVTGGGSEIQRARVGAYGSGELPQLNG
                     TGRDAVLLVDTSNLTVGDLDISNPGTGGPIGEGDQIRNGVRVIAQAGTVSDLTIGGLE
                     IHDVDGDLTKTPEGSAAIQISATGAAPVRFTGLHITGNQITSVSRSAISISGSNDLDR
                     PEASQAWPEASSGVVIKDNRIDQVAGDGIVTRGTDGALIEGNVVSHGNLAGRPLLDPE
                     GPMCNAGIWAFRANNTVIRGNEVFGMEHNGCDGTGFDVDYRQDGTVIEGNYSHDNEGG
                     FVLLCTDTATHRADVRFNLSVDDGTMINHGPCGIADGILGDLSGIRMFNNTVVGDSPT
                     TSIQLAVSDEMYQPGRFLFRNNLVYARQPAGQIACGNDCTHNAFFNLPPSGTHAVTAD
                     PQLIAGYRLGPKSPLRGAGMPITGGATTDYFGNPVPATPSIGFDQTPASRPAQPTPAC
                     RKARKARDRTIERAADLNRKLRKLRSKHAPRRQTRSVASRLGKARGEVKRLTRVARRA
                     CRPKGGAS"
     gene            complement(1803..2282)
                     /locus_tag="PLE93_02640"
     CDS             complement(1803..2282)
                     /locus_tag="PLE93_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH85977.1"
                     /translation="MAKKKKAGRVSAGLILYRVGPGGLEVLIAHMGGPHWARKERGWS
                     IPKGEIDPGEEPLETARREFREEVGIEPPAGAPIHLGEITQRSGKRVLAWAIEGDFDP
                     TQQTSVMCEIEWPPKSGEKIEIPEVDRVEWVPPATAKNLVIDGQIPLFDRLEKLVGR"
     gene            2355..>3607
                     /locus_tag="PLE93_02645"
     CDS             2355..>3607
                     /locus_tag="PLE93_02645"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HNH85978.1"
                     /translation="MNCRGWVALVAATVVLCAPGGASAGELTGLVNPFVGTEASAPDF
                     GTGGGAGNTFPGATMPFGMVAFGPDTIPSLTNFTAGYTYSDDQIRGFSLTHFSGAGCA
                     LLQDVPILPVARSLSGSPVVAGSSDIDPSIVPTFSHRHEEARPGFYSVVLNPGKADQI
                     RSELTATTRTAMGRFTFSKANTGTLTLNAGGSTMANYLAAVKVDPKAREVTGTSESGR
                     FCWEPSKYKVYFAARFDRPFKGYGTWKGAALSPGSKQASDESPDAFNYKPVAGGPPFI
                     PGNPSTTAQAGAFVSFNTSGSRPVGMRVGISFVSVAQARKNLSESAGLTFGEVRDRAG
                     QIWRQEMGKVRVSGGRKEDRRTFATALYHSLLEPSVQSDVNGLYRGQDLKVHRVAPGH
                     AHYSDISGWDVYRSQIQLLAMIEPRR"
BASE COUNT          522 a         1215 c         1211 g          659 t
ORIGIN      
        1 gaggacggct cgggcggcag cggctcgagt tcgatccgcc tggtccgccc caaacgccgt
       61 tagctggcgc cgcctttggg ccggcaggca cgacgggcga ccctggtcag tcgcttcacc
      121 tcgccccggg ccttgccgag ccggcttgcg accgatctgg tctgcctgcg gggcgcgtgc
      181 ttcgaacgga gcttcctcag tttccggttg aggtccgctg ctctttcgat cgtccgatcc
      241 cgggccttgc gggccttgcg gcaggccggc gtcggctgag cgggccggct ggccggggtc
      301 tgatcaaaac cgatcgacgg tgttgccggg accggattgc cgaagtagtc ggtggtggct
      361 cccccggtga tcggcatgcc ggctcctcgc agcggtgact tgggaccgag ccggtagccg
      421 gcgatcagct gcgggtcggc ggtgaccgcg tgcgtgccgc tgggcggaag gttgaagaag
      481 gcgttgtggg tgcaatcgtt tccgcaggcg atctgcccgg ccggttgacg ggcgtagacc
      541 aggttgttcc ggaagaggaa tctgcccggc tggtacatct cgtccgatac cgccagctgg
      601 atcgaggtgg tcgggctgtc gccgaccacg gtgttgttga acatccggat gccggaaagg
      661 tcgccgagga tgccgtcggc gatcccgcag ggaccgtggt tgatcatcgt gccgtcgtca
      721 acgctcaggt tgaaccgcac gtcggcccgg tgagtcgcgg tgtcggtaca gagcaggacg
      781 aagccgccct cgttgtcgtg gctgtagttg ccctcgatca ctgtgccgtc ctgccggtag
      841 tccacatcga agccggtgcc gtcgcagccg ttgtgttcca tgccgaagac ctcgttgccc
      901 cggatcacgg tgttgttggc ccggaaggcc cagatgcccg cgttgcacat cgggccttcc
      961 gggtcgagca ggggccggcc ggccaggttg ccgtggctta cgacgttgcc ttcgatcagg
     1021 gcgccgtcgg ttcccctggt gacgattccg tcaccggcaa cctgatcgat ccggttgtcc
     1081 ttgatcacca ctccggagga agcttccggc caggcctggc tggcttccgg ccggtcgagg
     1141 tcgttgctgc cggagatcga gatcgcgctc cgcgacaccg aggtgatctg gttgccggtg
     1201 atgtgcaagc ccgtgaaccg gaccggcgcg gcgccggtcg cgctgatctg gatcgcggcg
     1261 ctcccttccg gcgtcttggt caggtccccg tcgacgtcgt gaatctccag accgccgatc
     1321 gtgaggtcac tgacggttcc cgcctgcgcg atcacccgca ctccgttgcg gatctgatcg
     1381 ccctcaccga tcgggccgcc tgttccggga ttggagatgt cgagatcccc gaccgtcagg
     1441 ttcgaggtgt ccacgaggag caccgcgtcg cggcccgttc cgttcaactg cgggagctcg
     1501 cccgagccgt aggcgccaac cctggcccgc tggatttcgc tgcctccgcc ggtgacctcg
     1561 agccggccgt ggcaggtcgt gccgcgtttg agcagcaggc tgtcgccggg accgaggtcg
     1621 aaggcactgc cgagcgagtt gcgtggctgg gccggcgacc cgtctccgtc aatcgccgcc
     1681 gagcaatcga ggtaggtatc ggccgccgag gcagggacgg tcgcggtcgt tgcgagcacc
     1741 ccgaccgtca gccccaggct cagcaacttc gcggccgtct tcatcgggga gaagtctagg
     1801 ttctagcggc cgaccagctt ttcgaggcgg tcaaagaggg ggatctggcc gtcgatgacc
     1861 aggttcttcg cggtggccgg cggcacccac tcgacccggt cgacctcggg aatctcgatc
     1921 ttctcgcctg acttcggtgg ccactcgatc tcgcacatca cgctcgtctg ctgggtcggg
     1981 tcgaagtccc cctcgattgc ccaggccagc acccgcttgc cggagcgctg ggtgatctcg
     2041 ccaaggtgga tcggagcccc ggccggcggc tcgattccaa cctcttcccg aaactcgcgc
     2101 cgggccgttt ccagcggttc ctcgccgggg tcgatctccc ccttcgggat cgaccatccg
     2161 cgctctttcc gggcccagtg cggaccgccc atgtgggcga tcagcacctc gagacccccc
     2221 ggtccgacgc ggtagagaat cagtccggca ctgacccggc cggctttctt cttcttggcc
     2281 atcgggccga ggctaacttt cccggcgacc cgggcgctcc ctcgaaaggg ggtatggcgt
     2341 gtaatttgca ttgggtgaat tgcaggggat gggtcgcgct ggtcgcggca acagtggttt
     2401 tgtgtgctcc ggggggagcg agtgccggtg agttgaccgg tctggtcaac cccttcgtcg
     2461 gcacggaagc ctcggctcca gacttcggca ccggcggtgg tgcgggcaac acattcccgg
     2521 gcgcaaccat gcccttcggg atggtcgcct tcggtcccga cacgatcccc agcctgacca
     2581 acttcaccgc cggatacacg tattcggacg atcagatccg ggggttctcg ctgacccact
     2641 tcagcggtgc cggctgcgcc ctgctgcagg acgtgccgat cctgcccgtg gccaggagcc
     2701 tgtccggttc acccgtcgtc gccggttcct ccgacatcga cccgtcgatc gtcccgacct
     2761 tcagccatcg ccacgaagag gcccggcccg gtttctacag cgtggtcctc aaccccggca
     2821 aggccgacca gatccgctcg gaactgaccg caaccacccg caccgcgatg ggccgcttca
     2881 ccttctcgaa ggcgaacact gggaccctga ccctgaacgc gggtggcagc acgatggcca
     2941 actacctcgc cgcggtgaag gtcgatccga aagcccgtga ggtgaccgga acctcggaga
     3001 gcggtcggtt ctgctgggag ccgtcgaaat acaaggtcta tttcgcggcc aggttcgacc
     3061 gtccgttcaa ggggtacggc acctggaagg gcgcggccct ttctcccggg tcgaagcagg
     3121 ccagcgacga atcgcccgat gccttcaact acaagccggt cgcgggcggg ccaccgttca
     3181 ttcccggcaa cccgagcacc accgcccagg ccggggcctt cgtcagcttc aacacctcgg
     3241 gttcgcgccc ggtcgggatg cgggtcggga tctccttcgt cagcgtcgcc caggcgcgga
     3301 agaacctgag cgaaagcgcc ggcctcactt tcggcgaggt tcgcgaccgg gccggccaga
     3361 tctggcggca ggagatgggg aaggtgcgtg tttccggggg ccggaaggaa gaccgccgca
     3421 ccttcgcgac ggcgctctac cactccctgc tggaaccgag cgtgcagtcg gatgtcaacg
     3481 gcctctatcg cggccaggac ctcaaggtcc accgggtcgc cccgggtcac gcccattact
     3541 cggacatttc cggctgggat gtctaccgca gccagatcca gctgctcgcg atgatcgagc
     3601 cccggcg
//
LOCUS       DAOQFI010000099         3606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_11996_length_3606_cov_3.199944, whole genome shotgun sequence.
ACCESSION   DAOQFI010000099 DAOQFI010000000
VERSION     DAOQFI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11996_length_3606_cov_3.199944"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..748
                     /gene="trmD"
                     /locus_tag="PLE93_02650"
     CDS             89..748
                     /gene="trmD"
                     /locus_tag="PLE93_02650"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNH85979.1"
                     /translation="MNLDIFTLFPEAFDWFRQQRPVRKAIEHGAELNLRDYRDWTPLK
                     GGRVDDAPYGGGAGMVLRVDVVDAALRGVYGDGERPRTVVLSPAGRVLDDGLVTELAA
                     EPGLALLCGRYEGFDHRVHEHLADDEISIGRYVLAGGELPAMVLADTIMRRQPGALGD
                     EESAIEESWSEALEGLPEYPHYTRPPEYRGWGIPDVLISGDHAKVREWRLEQSRKRGA
                     E"
     gene            817..1158
                     /gene="rplS"
                     /locus_tag="PLE93_02655"
                     /pseudo
     CDS             817..1158
                     /gene="rplS"
                     /locus_tag="PLE93_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            1575..2156
                     /gene="lepB"
                     /locus_tag="PLE93_02660"
     CDS             1575..2156
                     /gene="lepB"
                     /locus_tag="PLE93_02660"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNH85980.1"
                     /translation="MVVIVALALGLALLIQAFLIKPYQIPSGSMEPTLDINQRILVNR
                     FVYHFTDPKPGDVVVFHPPAGVDRGGLEECGAPHQQDQPCPQPTSDKSSETFIKRIVA
                     GPGDTVSIEKGLPVVNGEVVEPDGYEINPCGPAGTGCNMPKEITVPDGHFFMMGDNRG
                     HSDDSRFWGPLPEEWLIGKAFATYWPPNRIGGL"
     gene            2146..2808
                     /locus_tag="PLE93_02665"
     CDS             2146..2808
                     /locus_tag="PLE93_02665"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNH85981.1"
                     /translation="MGSSTARTPAAEQAPGELFSHDLELGASAVAGADEAGRGCLAGP
                     IVAAAVLFDRECLESQGGGRLAEIRDSKKLTAKARERLYPEVLDCAVKTVVVMRSARY
                     IDANGLHVSNMECLARAIEGLGSGPEAVTLVDGFALKGCEVPHRRLVKGDSTSAAIAA
                     ASILAKVTRDRCMARAGLAYDGYGFEAHKGYASPAHRDAIRLLGPSPIHRLSFASEAY
                     QA"
     gene            complement(2846..3220)
                     /locus_tag="PLE93_02670"
     CDS             complement(2846..3220)
                     /locus_tag="PLE93_02670"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNH85982.1"
                     /translation="MDDRRATGRSAEDICARRLRERGWRILARNWRIRPGELDLIALA
                     GSTLVIVEVKAHHQGLRRGPVHPVLAVGPNKQRRIRRLASAWIAGHGRSIGFREVRFD
                     VVGITFDREGRLAAYDHLEDAF"
     gene            3384..>3606
                     /locus_tag="PLE93_02675"
     CDS             3384..>3606
                     /locus_tag="PLE93_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNH85983.1"
                     /translation="MLAETTSFTLDGIAARMIRVEVDVHRGLPNFQIVGLPDAAVREA
                     RERVRAATVNCGFDFPLQRITASLAPADLR"
BASE COUNT          625 a         1201 c         1212 g          568 t
ORIGIN      
        1 gccgtgcgcg aagagcgccg cgtgctggtc gacatcctgg actgacaccg atgcggtccg
       61 gccgcatacc cggcccggaa gcaggtcgat gaatctcgac atcttcaccc tctttcccga
      121 ggcctttgac tggtttcggc agcagcggcc ggtccgcaag gcgatcgagc acggcgcgga
      181 gctgaacctt cgggattacc gcgactggac tccgctgaag ggtggccggg tcgatgacgc
      241 cccttacgga ggcggggcag gaatggtgtt gcgggtcgac gtggtcgacg ccgccctccg
      301 gggtgtctac ggtgacggcg aaaggcccag gacggtggtt ctcagcccgg ccggccgggt
      361 actcgacgat ggcctggtca ccgaactcgc ggccgaaccg ggccttgccc tgctctgcgg
      421 tcgctacgag ggtttcgacc accgggtcca cgaacacctg gccgacgacg agatttcgat
      481 cggccgctac gtactggccg gtggcgagct gccggccatg gtgcttgccg acacgatcat
      541 gcggcgccag cccggcgccc tcggcgacga ggaatcggcg atcgaggaat cctggtccga
      601 ggccctcgaa gggctgcccg aatacccgca ctacacccgt ccacccgagt accggggctg
      661 gggcatcccc gacgtgttga tctccggcga tcacgcaaaa gtcagggagt ggcgactcga
      721 gcaaagccgc aagcgggggg cggaatgacg ctgcctgctc tgcttcggta ggattgcgcg
      781 tccgcgggcc ttcaaccggg tccgcttttt tgagccatga gcaccgtaat ccaggacatt
      841 gagaacagtc agctgaggaa gggacttccg tccttttccc cgggagatcg cgttcgcgtt
      901 cacttcctcg tcattgaggg aaaccgcaag cgcccgcagg tattcgaggg cgtggtgatc
      961 aagcgtcagg gctccggtgc ccgcgagact ttcactgttc gcaagcagtc cttcggcgtc
     1021 ggtgtcgagc gtactttccc gctccactcg ccgaagatcg agaagcttga agtcgcctcg
     1081 cgtggccggg tccgccgggc caagctctac tacctgcgcg gccgggtcgg caaggccgcc
     1141 cgggttgccg agcgtcgctg gggcattgac gaggacatgg tcggcacatc caaggccgtg
     1201 gacgcttcgg gtgaatccct cgaggagatc gaggaagcga ccgaagtaga ggccacggag
     1261 tcggacgctg ctcaggcaga agccacggaa accgccgaag cgaccgaaac cgccgaagcg
     1321 accggggccg aagctccgga agctcccgcc gacgagccgg ccgcggagga agctccggcc
     1381 ggggaagccg aagaggctcc cgccggggag cccgctgccg aggccgatga ggcgccggaa
     1441 gccgaagctg ccgaagaggc tcctgaggcc gaagcaactg aagagcccgc cgaagacggc
     1501 gagcagggcg agaaggaaga agcctgagcc cgaagatccc caagccgaaa acggccgggg
     1561 gtagcttcgt cgaactggtc gtaatcgttg cgctcgccct cgggctggcg ctgctgatcc
     1621 aggccttcct gatcaagccg taccagatcc cgtctggctc aatggagccg accctggaca
     1681 tcaaccagcg gatcctggtc aaccgcttcg tctaccactt caccgacccc aagccgggcg
     1741 atgtcgtcgt ctttcatccg ccagccggag tcgaccgggg cggcctcgag gaatgtggcg
     1801 ccccacatca gcaggaccag ccctgtcctc agccgacctc cgacaagtcg agcgagacct
     1861 tcatcaaaag aatcgtcgcc ggtccggggg acacggtctc gattgaaaag ggtttgcccg
     1921 tcgtcaacgg cgaggtggtc gagccggacg gctacgagat caacccctgc gggccggccg
     1981 gcacaggctg caacatgccg aaggaaatca ccgttcccga cggtcacttc ttcatgatgg
     2041 gcgacaaccg gggacacagc gacgacagtc gcttctgggg gcccctgccc gaagagtggc
     2101 tgatcggcaa ggccttcgcc acctactggc cgccgaaccg aatcggtggg ctctagcacc
     2161 gcccgaactc ccgcggcgga acaggctccg ggggagctgt tcagtcacga cctcgaactg
     2221 ggtgcttccg ccgtcgccgg ggccgacgag gccggccggg gttgccttgc gggcccgatc
     2281 gtggccgcgg cggtcctttt cgaccgcgaa tgcctcgaga gccagggcgg cgggcggctg
     2341 gcggagatcc gggactcgaa gaagctgacc gccaaggccc gcgagcggct ctaccccgaa
     2401 gtgctggact gcgccgtgaa gacggtcgtg gtgatgcgct cggccaggta catcgacgcc
     2461 aacggacttc acgtaagcaa catggaatgc ctggcccggg cgatcgaagg gctgggctcc
     2521 gggcccgaag cggtgaccct ggtcgacggg ttcgccctca agggttgcga ggtgccgcac
     2581 cggcggctcg tcaagggcga cagcaccagc gccgcgatcg cggccgcgtc gatcctcgcc
     2641 aaggtcaccc gtgaccgctg catggcccgg gccggcctgg cctacgacgg ctacggcttc
     2701 gaagcccaca aggggtacgc atcacccgct caccgggacg cgatcaggct gctcggaccc
     2761 tcgccgatcc accggctctc cttcgcttcc gaggcatatc aggcctgacc ggcagcgcga
     2821 tcgccccggg gctcgccgct tcacctcaga atgcgtcttc aaggtggtcg taggcggcca
     2881 gcctgccttc gcggtcaaag gtgattccga ccacgtcgaa gcggacttcg cggaacccga
     2941 tgcttcgccc atggccggca atccaggccg atgccagccg cctgatccgc ctctgcttgt
     3001 tggggcccac cgccaggacc ggatggaccg gcccccgcct cagcccctga tggtgggcct
     3061 tgacctcgac gatgaccagg gtggaaccgg caagggcgat caggtcgagc tcgcccggcc
     3121 ggattcgcca gttgcgggcc aggatccgcc agccccgttc ccggagtctg cgggcgcaga
     3181 tgtcttcggc gcttcgtcct gtggctcgtc ggtcgtccat ccttcgaaag tagggccggg
     3241 accggcgatc tgcggctgaa aacggtcttt tgttgcagtg aattcacccg ggtgtgagca
     3301 ttgccgtccg gaattcacgg aggcgtgcgg aatggttgca cccgtcgagc cacatgtgag
     3361 tgaattcggc ctagcttggc cccatgctgg ccgagaccac ctcattcacc ctcgacggga
     3421 tcgccgcccg gatgatccgg gtggaggtcg acgtgcaccg cggtctgccg aacttccaga
     3481 tcgtcggcct tcccgacgcg gcggtccgcg aagcacgcga gagggtccgg gccgcaacgg
     3541 tcaactgcgg cttcgatttt cccctgcagc ggatcaccgc cagcctcgcg ccggccgacc
     3601 tgagga
//
LOCUS       DAOQFI010000100         3596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12060_length_3596_cov_3.115504, whole genome shotgun sequence.
ACCESSION   DAOQFI010000100 DAOQFI010000000
VERSION     DAOQFI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12060_length_3596_cov_3.115504"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..610)
                     /locus_tag="PLE93_02680"
     CDS             complement(53..610)
                     /locus_tag="PLE93_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNH85984.1"
                     /translation="MEYNATPEKEVKRASGIFTGAGILMILVGCFAIVAPHIVGQAFA
                     LLVGISFVIASVFIFVLAFSAEGAGTVIARIAWALVALIVGLIIVTHTDLSAKAFTVI
                     LIAYFLAAGVVRLGAAFAQRGVPGAGWLGLNGALSLLIGLIFVASWPNSAEWAIGLLI
                     GIDLIFSGWTLILLSQEAKSQALGS"
     gene            655..726
                     /locus_tag="PLE93_02685"
     tRNA            655..726
                     /locus_tag="PLE93_02685"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gln,seq:ttg)
     gene            823..3144
                     /locus_tag="PLE93_02690"
     CDS             823..3144
                     /locus_tag="PLE93_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNH85985.1"
                     /translation="MRKLAVAVAVAVTTFFAQGIQAQAAVTDAFDGAITCKTQPAIPS
                     PLPGSEYAGQRWCGSKTSLPGLGSTVDSWDGTPIDVNFALPPEPGTGPDGNYPVVMIF
                     HGWGGMKSGFTDMQRWLSRGYAVFSMTDRGFHESCGSAAARLTGGTACDNGYIHLLDT
                     RYEVRDAQYLAGLLVDDGLISPTRIATTGGSYGGGLSMALAALKNRVMLPDGALTEWK
                     SPAGTDMSLAVAIPSIPWTDLAYSLVPNGNNLDYIEDASYYGRFGVMKESWVNLLYTL
                     GLGTGNYAAAGEDPGADLTGWKSVLDAGEPYDGQAGAQAILDEITAHHSSYYIDHSIQ
                     PAPLLIYSGFTDDLFPVDEATRFYNRTRAQYPNAKIGLIFGPNSGHMRGMSKSDATAY
                     QQEVENQWVDFYLKDEGAEPASNVRALLQTCPANAPVGDPYVADDWASISPGEIRLVS
                     PESQTVSASGGDPASAGIFNPAPTGQACSTAPGGKETGTASYELDPAPAGGFTVLGAP
                     TVVAKIQQEGVNSQIAARLVDVSPDGTTKLLVARGLWRPQGEGFQVFQLHANAWKVEE
                     GHVLRLELLPKDASVPAGLVANYGRPSNGQTDATVTDLDLRIPVADQPGAAGGLVTAP
                     AAKVLPDRPGVKLAPGYDTDDQVPIDDYEPPPVDHPKTIRKRIVIGKVQIKGKRVLIK
                     AGCPKAATVCPKARITVAGAKRKTRGTLARIKGVVVKPGENRLVSTRLTKRGQKVLKQ
                     TRGRKLAVKVTVKPAGMAKRTVRRVARVTRQRR"
     gene            3290..>3596
                     /locus_tag="PLE93_02695"
     CDS             3290..>3596
                     /locus_tag="PLE93_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85986.1"
                     /translation="MTGPVQAAAPANILDGQIPCGVVTDEGSGGSAVSTSLGQVWCGT
                     IRPSDNTSATVTPAIESVRSTAKTTDGVPLDINFAMPDPGTFGPPPYPTLMAFHGYGG
                     "
BASE COUNT          675 a         1211 c         1152 g          558 t
ORIGIN      
        1 gggttgaagt tattcgttcc atgtgcgaat aacttcaatg cagatgggag cgttagctgc
       61 cgagggcctg ggacttggct tcctgggaga ggaggatcag ggtccagccg gagaagatca
      121 ggtcgatgcc gatcaggagg ccgatcgccc actcggccga attcggccag gaggcgacga
      181 agatcaggcc gatcaggagc gaaagtgctc cgttcaagcc aagccagccc gccccgggca
      241 cgccccgctg ggcgaaagcg gctccgagcc ggacgacgcc ggccgcgagg aagtaggcga
      301 tcaggatcac ggtgaaggcc tttgcgctca ggtccgtatg ggtgacgatt atcaacccga
      361 ctatcaatgc gaccagggcc caggcgatcc tcgcgatcac ggttccggct ccctcggccg
      421 agaaagccaa gacgaagatg aacaccgacg caatgacgaa agagatcccg accagcagcg
      481 caaatgcctg accgacgatg tggggcgcga cgatcgcgaa acaaccgacc aggatcatca
      541 ggatcccggc cccggtgaaa atcccggatg cgcgtttgac ttccttctcc ggtgttgcgt
      601 tgtattccat ggccaaagcc tatcctcgcc agcggaggta gagtccggcg cgattccggg
      661 atggtgtaat cggtagcaca ccggtttttg gtaccggcag tggaggttca agtcctcctc
      721 ccggagtttg gagtacctct atgtttggga atcgcgggac gcggttatgg gtgcagggtc
      781 agtctcgttt tcaggcaata gggacatcac ccggaggaat cgatgcgaaa gctcgcagtc
      841 gcagttgcgg tagccgtcac caccttcttc gcccagggca ttcaggccca ggccgcggtc
      901 accgacgcct tcgacggcgc gatcacctgc aagacccagc cggcgatccc ctctccgctg
      961 cccggcagcg agtacgccgg acagcggtgg tgtggctcga aaacttcgct gcccgggctt
     1021 ggcagcaccg ttgattcctg ggatggaacg ccgatcgacg tcaacttcgc cctgccaccc
     1081 gaaccgggaa ccgggccgga cggcaactac ccggtggtca tgatcttcca cggctggggc
     1141 ggcatgaagt ccggtttcac cgacatgcag cgctggctca gccgcggcta cgcggtcttt
     1201 tcgatgaccg accggggatt ccacgaatcc tgcggcagcg cggccgcccg cctgaccggc
     1261 ggcaccgcct gcgacaacgg atacatccac ctgctcgaca cccgctacga agtgcgcgac
     1321 gcccagtacc tggccggtct gctcgtcgat gacggcctga tctcgccgac ccggatcgcc
     1381 accaccggcg gctcctacgg cgggggtctc tcgatggccc tcgcggccct gaagaaccgg
     1441 gtaatgctgc cggacggcgc cctgaccgaa tggaagagcc cggccgggac ggacatgagc
     1501 ctggccgtgg cgatcccgag cattccctgg accgacctcg cctactcgct cgtcccgaac
     1561 gggaacaatc tcgactacat cgaggacgcg tcgtattacg ggcgcttcgg cgtgatgaag
     1621 gagtcctggg tcaacctgct ctacacgctc ggactgggaa ccgggaacta cgcggctgcc
     1681 ggcgaggatc ccggcgccga cctgaccggc tggaagagcg tgctggacgc cggcgagccg
     1741 tatgacggcc aggccggggc ccaggcgatc ctcgacgaga tcaccgctca ccactcctcg
     1801 tattacatcg accacagcat ccagcccgcc ccgctcctga tctactcggg cttcaccgac
     1861 gacctcttcc cggttgacga ggcgacccgc ttctacaacc ggacccgggc ccagtacccg
     1921 aacgcgaaga tcgggctgat cttcggaccg aactcgggtc acatgcgggg catgagcaag
     1981 tccgatgcga ccgcctacca gcaggaagtg gagaaccagt gggtcgactt ctacctcaag
     2041 gacgaaggcg ccgaaccggc ctccaacgta cgggccctgc tccagacctg cccggcaaac
     2101 gcgccggtcg gtgaccccta tgtcgccgat gactgggctt cgatctcccc cggcgaaatc
     2161 cggttggtct caccggaatc ccagaccgtt tccgccagtg gcggcgatcc cgcgtccgcc
     2221 gggatcttca atcccgcccc gaccggccag gcctgctcga ccgcgcccgg cggcaaggaa
     2281 accgggacgg ccagctacga gctcgacccg gccccggccg gtggcttcac cgtgctcggc
     2341 gctccgaccg tggtcgcgaa gatccagcag gaaggcgtca actcgcagat cgcggcccgg
     2401 ctggttgacg tctcgcccga cgggacgacc aagctgctgg tcgcccgtgg cctctggcgc
     2461 ccgcagggtg agggtttcca ggtcttccag ctccacgcca acgcctggaa ggtcgaggag
     2521 ggccacgtgc tgcgactcga actgctgccg aaggacgcct cggtgccggc cggcctcgtc
     2581 gcgaactacg gccgcccctc gaacggccag accgatgcca ctgtcaccga cctcgacctg
     2641 cggatcccgg tcgccgacca gcccggcgcg gccggcggtc tggtcaccgc gccggccgcc
     2701 aaggtcctgc ccgaccggcc cggcgtgaag cttgcccccg gctacgacac cgacgatcag
     2761 gttccgatcg acgattacga gccgccgccg gtcgaccacc cgaagacgat caggaaacgg
     2821 attgtgatcg gcaaggttca gatcaagggc aagcgagtgc tgatcaaggc cggttgcccg
     2881 aaggcagcaa ccgtctgccc gaaggcgcgg atcaccgtgg ccggcgcaaa gcggaagacc
     2941 cgcgggacac tggccaggat caagggagtg gtcgtcaaac ccggcgaaaa ccggctggtt
     3001 tcgacccgcc tgaccaaacg cgggcagaag gtcctgaaac agaccagggg ccggaagctg
     3061 gcggtgaagg tcaccgtcaa accggccggg atggcgaaga gaacggttcg acgggtagcg
     3121 agggtcaccc gccagcgcag gtagggttcc ccgcggaaat ctgccggctt gcatatatct
     3181 ttgatcagcg ctacgatccc cgcggttcgg caagtggccg ggagtacgac cgggaggggt
     3241 tgaagtggtg cggagagccg ttgttcttgc agtgttcgtg ttctttggcg tgaccggacc
     3301 cgtgcaggcg gcggcgcccg cgaacatcct ggacgggcag attccctgtg gcgtcgtgac
     3361 cgatgaaggt tcaggcggca gcgccgtcag cacctccctg ggacaggtct ggtgcggcac
     3421 gatccgcccc tccgacaaca ccagcgcaac cgtcaccccc gcgatcgaat cggtccgcag
     3481 caccgcgaag accaccgacg gcgttccgct ggacatcaac ttcgcaatgc ccgaccccgg
     3541 cactttcggc ccgcccccgt accccaccct gatggctttc cacggatacg gcggaa
//
LOCUS       DAOQFI010000101         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12091_length_3591_cov_2.882919, whole genome shotgun sequence.
ACCESSION   DAOQFI010000101 DAOQFI010000000
VERSION     DAOQFI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12091_length_3591_cov_2.882919"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLE93_02700"
     CDS             <1..766
                     /locus_tag="PLE93_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH85987.1"
                     /translation="PAFTGENPPAAALAILEPKPLFDPFNPDTRATAGTGGHWILNGV
                     KSMVARPGECELFIVAAATGDARPTLFIIESGSEGLTFEAEPAMGLRAASTGRLVLED
                     VRVPASAMLGEEGDRARIYADCVSGSRIAWSAMFSGTAQAVLDYVIPYVNERQAFGEP
                     ISNRQSVAFSVSDIAIETGGMRLANWRAASRADRGESFAREAALARRLAAAKGVEIGS
                     AGVQLLGGHGYVKEHPVERWYRDLRAAGVMEGALLA"
     gene            792..2009
                     /locus_tag="PLE93_02705"
     CDS             792..2009
                     /locus_tag="PLE93_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH85988.1"
                     /translation="MIYLETPKKFRPLVTQANHVAREIFRPISRKYDEAEHEYPKELD
                     VLASLINGMNEGSGMGAGAAGVSGESGDEPDSGSGNRNGSNLSTVLGIIELCWGDVGL
                     LLAMPGQGLGNSAIASVATAEQLERYGGKWAAMAITEPEAGSDSAAIRTTAVLDGDEY
                     VLNGEKIFVTSGDRAELIVVWASLDRSVGRAAIKSFIVERDNPGLTLDRLEHKLGIRA
                     SDTANFTLQDCRVPKEALLGSPEVDVKKGFAGVMKTFDNTRPLVAGMALGVTRAALQE
                     TKRQLEAAGVEIDYDTPALCQSAAAARYLEMEADYEAALMLTLQAAWMADNRKPNSLE
                     ASMAKAKAGRTGVDVTLGCVEICGSIGLGESELLEKWSRDAKILDIFEGTQQIQLLII
                     ARQLLDKTSAELK"
     gene            complement(2025..>3591)
                     /locus_tag="PLE93_02710"
     CDS             complement(2025..>3591)
                     /locus_tag="PLE93_02710"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNH85989.1"
                     /translation="TRLNSVIVRNAEARAEAAASDARRANGQAMGPLDGIPYTAKDSY
                     LVRGMTAASGSPAFVNLLAMRDAFTVKRLREGGAICLGKTNMPPMANGGMQRGVYGRA
                     ESPYNADYLTAPFASGSSNGAGTATAASFAAFGLAEETWSSGRGPASNNGLCAYTPSR
                     GVISVRGNWPLTPTMDVVVPYARTMADLLEVLDVIVADDPDTRGDLWRAQEWIELPAA
                     SEVRPDSYPSLAASSEALAGNRFGIPRMYINADPAAGTGEKSDVWGPTGERIDTRQSV
                     IDLWQAARNDLEAAGAEVVEVDFPVVSNYEGDRAGCPNIFTRGLVSPAFLDEELRGLS
                     AWAYDDFLRANDDPALNRLADADGELIFPPEAGALPSREGDLAFGMEEYVEMAWAGLT
                     PWDQLPELEAGLRGLEQTRRIDLEEWMDELGLDTVVFPAVADVGPADADVNPRSADRA
                     WANGVWVANGNLVPRHLGIPTVTVPMGLMGDIGMPVGLTFAGRSYDDNRLLTQASAFE
                     SIRPRRIPPPRTT"
BASE COUNT          600 a         1230 c         1209 g          552 t
ORIGIN      
        1 gcccgccttc accggcgaaa atccgccggc cgccgcgctg gcgatcctcg agccgaagcc
       61 gctctttgat cccttcaatc ccgacacccg ggccaccgcc ggaaccggcg gccactggat
      121 cctcaacggg gtgaagtcga tggtcgcccg gcccggcgaa tgcgagctct tcatcgtcgc
      181 ggccgccacc ggtgacgcca ggccgacgct cttcatcatc gaatccggct ccgagggcct
      241 gaccttcgaa gccgaacccg cgatggggct cagggccgca tcgaccggcc ggctggtgct
      301 ggaagatgtg cgcgttcctg cctcggcaat gcttggcgag gaaggcgacc gcgcccggat
      361 ctacgccgac tgcgtttcgg ggtcccggat cgcctggtcg gcgatgtttt ccggcaccgc
      421 ccaggccgtg ctcgactacg tgatccccta cgtgaacgag cgtcaggcct tcggcgagcc
      481 gatctcgaac cgccagagcg tggccttcag cgtttccgac atcgcgatcg aaaccggtgg
      541 catgcgcctc gccaactggc gggccgcgag ccgggccgac cgcggggaat ccttcgcccg
      601 cgaagcagcc ctggcccgcc ggctcgccgc cgcaaagggc gtggaaatcg gctcggccgg
      661 ggtccagctg ctgggcggcc acggctacgt caaggagcac ccggtcgaac gctggtatcg
      721 cgacctccgc gcagcgggcg tgatggaggg cgccctgctc gcctgatcac caggccgagc
      781 gaaaggaaac catgatctat ctggaaacac cgaagaagtt ccgcccgctg gtcacccagg
      841 ccaaccacgt cgcccgcgag atcttccgtc cgatctcgcg caagtacgac gaggccgaac
      901 acgagtaccc gaaggaactc gacgtactgg cctcgctgat caacggcatg aatgagggtt
      961 cgggcatggg cgccggtgcg gccggcgtca gcggggaatc cggtgatgaa ccggattcgg
     1021 gcagcggcaa ccgcaacggt tcgaacctga gcaccgtgct cggcatcatc gagctctgct
     1081 ggggtgacgt cggactgctt ctggcgatgc ccggccaggg gctcggcaac tcggcgatcg
     1141 cgtcggtcgc gaccgcggag cagctcgagc gctacggcgg caagtgggcc gcaatggcga
     1201 tcaccgaacc ggaggcgggc tcggattccg cggcgatccg cactacagcc gtcctcgacg
     1261 gggacgaata cgtgctgaac ggcgagaaga tcttcgtcac ttccggtgac cgggccgagc
     1321 tgatcgtggt ctgggccagc ctcgaccgct cggtcggccg ggcggcgatc aagtcgttca
     1381 tcgtcgagcg tgacaatccg ggcctgaccc tggaccggct cgagcacaag ctgggcatcc
     1441 gggcgtcgga taccgccaac ttcaccctgc aggactgccg cgtgccgaag gaggcgctgc
     1501 tcggctcccc ggaggtggac gtgaagaagg gcttcgccgg agtgatgaag accttcgaca
     1561 acaccaggcc cctggtcgcc ggcatggccc tcggggtaac ccgcgcggcg ctccaagaga
     1621 cgaagcgcca gctcgaagcg gccggtgtcg agatcgatta cgacactccg gcgctttgcc
     1681 agtccgcggc cgcggcccgc tacctcgaga tggaagccga ttacgaggcg gccctgatgc
     1741 tgaccctgca ggccgcctgg atggccgaca accggaagcc gaactcgctc gaagcgtcga
     1801 tggccaaagc caaggccggc cggaccgggg tcgacgtgac cctgggctgc gtcgagatct
     1861 gcggcagcat cggtctcggt gaatccgagc tgctcgagaa gtggtcccgt gatgcgaaga
     1921 tcctcgacat tttcgaaggc acccagcaga tccagctgct gatcatcgcc cgtcagctgc
     1981 tcgataagac ctcggccgaa ctcaagtaac cagaagcccc cggctcaggt ggtccgcggt
     2041 ggcgggatgc gtcgggggcg gatcgattcg aaagccgagg cctgggtgag cagccggttg
     2101 tcgtcgtatg aacgaccggc gaaggtgagg ccgaccggca tcccgatgtc acccatcagc
     2161 cccatcggca cggtcacggt cggaatgccg aggtgccggg gaacgaggtt gccgttggcc
     2221 acccagacac cgttggccca ggcccggtcg gccgagcgcg ggttcacatc ggcgtcggcc
     2281 ggaccgacgt cggcgaccgc cggaaagacc accgtgtcca gtccgagctc atccatccac
     2341 tcctcgaggt cgatccggcg ggtctgctcc agaccccgca ggcctgcttc gagttcaggc
     2401 agttgatccc agggggtgag gccggcccag gccatctcca catactcctc catgccgaag
     2461 gccaggtcac cttcccggct gggcagggca ccggcttcgg gcggaaagat cagctcgccg
     2521 tccgcgtccg ccaaccggtt cagggccggg tcgtcattgg cccggagaaa gtcgtcgtac
     2581 gcccaggccg aaaggccgcg caactcctcg tcgagaaagg ccgggctgac caagccgcgg
     2641 gtgaagatgt tcggacagcc ggcccggtca ccctcgtagt tggagaccac cgggaaatcg
     2701 acttcgacca cctcggcccc ggccgcctcg agatcattgc gggctgcctg ccagaggtcg
     2761 atcaccgact gccgggtatc gatgcgctcg ccggtcggtc cccagacgtc gctcttctcc
     2821 cccgtgcccg cggccggatc ggcgttgatg tacatgcgcg ggataccgaa ccgattgccg
     2881 gccagcgcct cggaagatgc ggccagcgac gggtacgagt ccggtctgac ctcggaggcg
     2941 gccggcagct cgatccactc ctgcgcccgc cagaggtctc ccctggtgtc gggatcatcg
     3001 gcgacgatca cgtcgagcac ctcgagcaga tcggccatgg ttcgcgcgta gggaaccacc
     3061 acgtccatgg tcggggtaag cggccagttg ccccggaccg aaatcactcc gcgtgaaggc
     3121 gtgtaggcgc agaggccgtt gttcgaggcc ggccccctgc cgctcgacca ggtttcctcg
     3181 gccagaccga aggcggcgaa actggccgcg gtcgcggtcc cggccccgtt cgatgatccc
     3241 gaggcgaacg gcgcagtcag gtaatcggcg ttgtaggggc tctccgcgcg gccgtagacg
     3301 ccccgctgca tgcccccgtt ggccatcggc ggcatgttgg tcttgccgag gcagatcgcg
     3361 cccccttccc gcagccgctt gaccgtgaag gcgtcacgca tcgccagcag gttgacgaag
     3421 gccgggctcc cggaggcagc cgtcatgccg cgcaccaggt agctgtcctt agcggtgtag
     3481 gggattccgt caagcgggcc catggcctgg ccgttggccc gcctggcatc ggacgcggcc
     3541 gcttccgccc tggcttccgc gttccggacg atgaccgagt tcagcctggt t
//
LOCUS       DAOQFI010000102         3589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12107_length_3589_cov_3.138936, whole genome shotgun sequence.
ACCESSION   DAOQFI010000102 DAOQFI010000000
VERSION     DAOQFI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12107_length_3589_cov_3.138936"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1718
                     /locus_tag="PLE93_02715"
     CDS             141..1718
                     /locus_tag="PLE93_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85990.1"
                     /translation="MALASGFILYYGRDGWFSIDEAQWISDSPQLDLGGALSPHVGHL
                     VFIPRLVYKLMLETVGIEYIAYRILTVLAILLCTALFFTWARRRLPDFVALAFSLPIL
                     LFAYDPLHLIAGNGFTVMLALSFGIAALLAWDREDRTGDIWTFAFLVLGGLTYTVALP
                     FSVAIAFAAVLDRRRDRLWVGAVPIVLYLAWRTLGDVGATDTAAGGGDLLNILLLPAW
                     SFGGISGVLASWSGLSYDFIRSGTMQPGTGIGPALAVVAIAALGWRIATRGAGTKLWT
                     VIAVAIALFAAQTIAVGGIERYPEMPRYMYPGLIVVFLVVVEAASGFKWSRAAFIGLW
                     LITVISLMTSFSLLRDQSEVREWKATQTRVEVTAVGLLDQTPNPPPVIRQRRRMLTDD
                     FNPVTGSAYGALGYDPATLKDRDPDIGNAVDRFLFNALKIGFQPVTGSFSRADCRPLE
                     AIGGRYRMWVPRGGAVVIGKQDAKVWIGRFGTGRKLRAGRVGPNSPQLLKLRYDGEPT
                     PWFLKSKAPGVSVCPID"
     gene            1766..2836
                     /locus_tag="PLE93_02720"
     CDS             1766..2836
                     /locus_tag="PLE93_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNH85991.1"
                     /translation="MPDTISFARGAPSPDLIPVEAVRVAAAKALEDDWQKALSYGVGI
                     GHPGLCAWIADRHGIDPSQVMTTNGSLEAGAMLFRHLLSPGDRVVVEQPSYDRTLLLL
                     EQIGVVFVPIALEEDGIDVDALEKALELGPIKLAHIIPNFHNPAGCTLSEEKRRRLVA
                     LAAEHDFVIFEDDPYRELPFGEDPPPTMLSMDTADKVIHASSFSKTVSPGVRVGYLAG
                     PEHEIARLAKIANENYISPNMLSGSLVLELCQSGVLDRNIAEVKVALQERRDALVEAL
                     GKHIPEATFVLPGGGYFLWADLAEDRDTVQIAAKAKEAGAPFIPGVDFMLDAGQRSLR
                     FSFASVPPEHMDEGVSRIASCL"
     gene            2967..3197
                     /locus_tag="PLE93_02725"
     CDS             2967..3197
                     /locus_tag="PLE93_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85992.1"
                     /translation="MSKMVQIRDVPDEIHALLVERAASEGRSLSEMLREEVATLARRP
                     SRAEVLKRIRARGRIETPETGAEAIRRLREDD"
     gene            3194..3580
                     /locus_tag="PLE93_02730"
     CDS             3194..3580
                     /locus_tag="PLE93_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH85993.1"
                     /translation="MIVVDASVILDLLRLAEGSTSTEIALVSGEPLAAPDHMLVETTR
                     VLRRWQLGGAFDAETASEMVIQALDLGIRSYSCGDLIPRAWELRNQFTLDDALYIALA
                     EALPARLLTSDQKLASQASRFVEIYE"
BASE COUNT          570 a         1234 c         1141 g          644 t
ORIGIN      
        1 cggggcctta cacacggcgt ttacatccgg aagccgaaac ccgcgaaaat accccggtgt
       61 ctccgtttcg ccagagaatc gatcgcctga gacagggcga gaacagcccc cgcgcgccct
      121 ggttggtgct cgcgggtgcg atggccctgg cgagcgggtt catcctctac tacgggcgtg
      181 acggctggtt ctcgatcgac gaggcccagt ggatctccga cagcccgcaa ctcgacctcg
      241 gcggggcgct ttccccccac gtcgggcatc tggtcttcat tccgcggctg gtctacaagc
      301 tgatgctgga gacggtgggc atcgagtaca tcgcctaccg catcctcacc gtgctggcga
      361 tcctgctctg cacggccctc ttcttcacct gggcccggcg ccggctcccc gacttcgtcg
      421 ccctggcctt cagcctgccg atcctgctct tcgcctatga cccgctccac ctgatcgccg
      481 gcaacggttt cacggtgatg ctcgccctct ccttcgggat cgccgcgctc ctggcatggg
      541 accgcgaaga ccgaaccggg gacatctgga ccttcgcctt cctggttctg ggaggtttga
      601 cctacacggt cgccctgccc ttctcggtgg cgatcgcgtt cgccgcggtt ctcgaccgcc
      661 gccgggaccg gctctgggtg ggagcggtgc cgatcgtcct ctatctcgcc tggcggaccc
      721 tgggtgatgt cggtgccacc gacaccgccg cgggcggggg cgacctgctc aacatcctgc
      781 tgctgccggc ctggagcttc ggcggcatct ccggtgtgct cgcttcctgg tccggcctga
      841 gttatgactt cattcgctcc gggaccatgc agccgggaac cgggatcggc cccgccctcg
      901 cggtggtggc gatcgcggcg ctcggctggc ggatcgccac ccgcggagcg ggcacgaagc
      961 tctggacggt gatcgccgtc gcaatcgcgc tcttcgccgc ccagacgatc gcggtcggcg
     1021 ggatcgagcg ctacccggag atgccgcgct acatgtatcc cggcctgatc gtggtgttcc
     1081 tggtcgtggt cgaggcggcc agcggcttca agtggtcacg ggccgcgttc atcgggctct
     1141 ggctgatcac ggtcatcagc ctgatgacct cgttctcgct gcttcgcgac cagtccgagg
     1201 tcagggagtg gaaggcgacg cagaccaggg tcgaggtgac ggcggtcggc ctgctggacc
     1261 agaccccgaa cccgcccccg gtcatccggc agcggcggcg catgctgacc gatgacttca
     1321 acccggtgac cggttccgcc tacggcgccc tcggctacga cccggcgacc ctgaaggacc
     1381 gggatccgga catcggcaac gccgtcgacc gcttcctctt caacgcgctg aagatcggtt
     1441 tccagccggt gaccgggtcc ttctcgcggg cggattgcag gccgctggag gcgattggcg
     1501 gccggtaccg gatgtgggtg ccgcggggcg gcgccgtggt gatcggcaag caggacgcga
     1561 aagtctggat cggccggttc ggcaccgggc ggaagctcag ggccggcagg gtcgggccga
     1621 attcgccgca gctcctcaaa cttcgctacg acggggaacc gactccctgg ttcctgaaga
     1681 gcaaggcccc cggcgtctcg gtctgtccga ttgactaaca tcgcctctcc cgcccagcga
     1741 ccgctccgag agcgagagtg acgaaatgcc tgacacgatc agttttgccc gaggtgcccc
     1801 cagtcccgac ctgattccgg tcgaggcagt ccgcgttgcc gcggcgaagg cgctcgagga
     1861 cgactggcag aaggcgctct cctacggggt cgggatcggc catcccgggc tttgcgcctg
     1921 gatcgccgac cgtcacggca tcgacccctc ccaggtgatg accaccaacg gttcgctgga
     1981 ggccggtgcg atgctcttcc ggcacctgct ttcacccggt gaccgggtcg tggtcgagca
     2041 accgagttac gaccgcaccc tgctcctgct cgagcagatc ggggtcgtct tcgtgccgat
     2101 cgcactcgag gaagacggaa tcgacgtcga tgcgctcgag aaggccctcg aactgggccc
     2161 gatcaagctc gcccacatca tccccaactt ccacaacccg gccggctgca ccctctccga
     2221 agagaagcgc cgccggctgg tcgcccttgc cgccgagcat gacttcgtga tcttcgagga
     2281 cgacccctac cgcgagctgc ccttcggcga ggaccccccg ccgaccatgc tctcgatgga
     2341 cacggccgac aaggtcattc acgcctcatc cttctcgaag acggtctccc cgggcgtccg
     2401 ggtcggctac ctggccggtc ccgaacacga gatcgcccgt ttggcgaaga tcgccaacga
     2461 gaactacatc tcgcccaaca tgctttccgg ttcgctggtt ctcgagctct gccagtcggg
     2521 tgttctggac cggaacatcg cggaagtgaa ggtcgctctc caggagcgac gcgacgccct
     2581 ggtcgaggcc ctcggaaagc acatccccga agccaccttc gtcctgcctg gcggcggcta
     2641 cttcctctgg gccgacctgg ccgaggaccg tgacacggtc cagatcgccg ccaaggccaa
     2701 agaggccgga gccccgttca tccccggggt cgacttcatg ctcgacgccg gccagcgcag
     2761 cctccgcttc tccttcgcct cggtcccccc ggagcacatg gacgaaggcg tctcccgcat
     2821 cgcctcctgc ctgtaggcac attcccgcac caaccaggcg tccggcccga gaggtgccgc
     2881 acatccagga ccgtgatggg ccaaaacctg cggcacctcc agcgattcct tcccggcgca
     2941 tgcgattcgc atgctagcct tggcttatga gcaagatggt tcagattcgc gatgtgccgg
     3001 acgagatcca cgcgctcctg gttgaaaggg ccgccagtga gggtcgatcc ctttccgaga
     3061 tgctgcggga ggaagttgcc accctggcaa ggcgacccag tcgcgcagag gtgttgaaac
     3121 ggatcagggc aagaggaagg atcgaaaccc cggaaaccgg ggcggaagcc atccgaaggc
     3181 tgcgggaaga cgattgatag ttgtcgatgc gagtgtgatc ctcgacctgc ttcgtctggc
     3241 ggagggctcc acttcgaccg aaattgcgct cgtttccgga gagcccctcg ccgcgcccga
     3301 tcacatgctg gtcgaaacaa cgcgggtgct tcggcgctgg cagctgggcg gcgcattcga
     3361 cgcggagacc gcgagcgaga tggtgatcca ggcgctcgat cttggcatca ggagttattc
     3421 atgtggcgac ctgatcccca gggcctggga gttgcgaaac cagttcaccc tggatgacgc
     3481 cctgtatatc gcccttgcgg aggcattgcc ggcgaggctc ctcacctccg atcaaaagct
     3541 ggccagccag gcgtcccggt tcgttgagat ctacgagtag gtctgtgcc
//
LOCUS       DAOQFI010000103         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12231_length_3565_cov_3.438177, whole genome shotgun sequence.
ACCESSION   DAOQFI010000103 DAOQFI010000000
VERSION     DAOQFI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12231_length_3565_cov_3.438177"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLE93_02735"
     CDS             <1..331
                     /locus_tag="PLE93_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HNH85994.1"
                     /translation="LCATGHSFRVAISDPTIVVDGANSRIIADVDSNLTGVWTPAQRI
                     DVATLNLAGGTTSLLSGTLGTKWESVPAAMTENGGKAICGVTEPQCPYSTGTAMDPVT
                     VEARAGI"
     gene            413..1702
                     /locus_tag="PLE93_02740"
     CDS             413..1702
                     /locus_tag="PLE93_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HNH85995.1"
                     /translation="MTSTRAALYGFALVAVLLAVFVGFGSRPALAGAATLEPCEATNP
                     TGPIVELDGQLTLTPVRSTRRAWKRSGISQRVTRPASALTGRPTFPVTGVRYGKSARV
                     KLGGGLRISHGRRALRISDLMVVSPAGKPARIDGRLGRTRHTLFRVKAGKRIFNAASG
                     KLSKVGSARLTAGGARVLNRNLRLRRSRLKAGLVWGYMNLYSLYKVTQVDDPTGEVPE
                     VPPEKERPIEAKTVASAATVKWFVRDSFINYVASGEGTRVEDGATADAPSGPDHLVYS
                     FNFPFDSGWTVPEAIDSPENTVIRGTGLVGFRYCHNTINFTAASPVIEIDGDANSRLI
                     FTVHGTDGTAFPEQPAVMVKLIPSRAQSHTVVDNMNGYSTVTWEKIPGYVPAEGTGIF
                     AGFYPAFSPDYEGQPQTERPDRFGYFSITYTFPNTDH"
     gene            1699..2889
                     /locus_tag="PLE93_02745"
     CDS             1699..2889
                     /locus_tag="PLE93_02745"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNH85996.1"
                     /translation="MSQVDPSRTSTSGRILAFGAALLATVVGSMSLAAQSPAAIQEGI
                     VEVNGYAWTVNPVAVDLNDIAPTNNGSSRYTVLEILEEGQNGQYRISQIPRVTINLPG
                     KLTGPGCSGDQIRGNSNQCPTFRSTPDATIMSYKAGSQTRQVEYRDYNPQIYIEKADD
                     MSVDLSPPSAKIDSGGQVRFTTDVSGDIGAVTYEWDFGDGTKKTTSKGSISHTFKGSN
                     RTYTVVVSVTSTGNPRSADDLSTITVGKVKKKPADQNPNQKNPDNSDNGGGGETTEST
                     PYVPGYSGGSTGGTGGGSPGASPNTSPGQPKREKQQPANESSGQTVTGQLIDPASIAT
                     ATPSTGDSTAATEQASPDDGSGGGGIPDGAKAALGIGALLGLGGLAEAGAFTGAFRRI
                     RFRL"
     gene            complement(2899..>3565)
                     /locus_tag="PLE93_02750"
     CDS             complement(2899..>3565)
                     /locus_tag="PLE93_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH85997.1"
                     /translation="VDGNGVIEPLLERVDREGITLTHILLTHEHWDHVVDLREVADRY
                     GVPIVASQKTADLVDFKVDQIVEDGDETVSGGLTIRWIATPGHSDGHMALLINGTDVI
                     TADVIFKGTVGGTVAPGETGFPELKSSIMDRLMTLPPETRIHPGHREPSTVGEEWENN
                     PFIRVWRGLDEEGDDPCEVNNFGPATLILWAPDYDGTNKAWIRLPDGEDKITGGSQIL
                     SRG"
BASE COUNT          661 a         1228 c         1126 g          550 t
ORIGIN      
        1 gctctgcgcg accggccaca gcttccgcgt cgcgatctcc gatccgacga tcgtcgtcga
       61 cggggcgaat tcgcggatca tcgccgatgt cgattcgaac ctgaccgggg tctggactcc
      121 ggcccagcgg atcgacgtcg ccacgctgaa cctggccggc ggcacgacct cactgctcag
      181 cggcaccctg ggcaccaagt gggagagcgt tcccgccgcg atgaccgaaa acggcggcaa
      241 ggcgatttgc ggcgtcaccg aaccgcaatg cccctacagc accggcaccg cgatggaccc
      301 ggttacggtc gaggccaggg ccggcatcta accttcggga acttcgctcg attcgctgga
      361 gaccgacaag ggaccgattc tgagtcgcag gacctcatat tccgtcactg atgtgacctc
      421 cacgagggcg gccctgtacg ggttcgccct cgtcgcggtt ctgctcgcgg ttttcgtcgg
      481 cttcggttcc cgaccggcgt tggccggggc cgcgaccctc gagccctgcg aggcgaccaa
      541 tccgaccggg ccgatcgtcg aactcgacgg ccagctcact ctcaccccgg ttcggtccac
      601 ccgcagagcc tggaagcgca gcggcatcag ccaacgggtg accaggccgg ccagcgccct
      661 gaccggtcgc ccgacctttc cggtgaccgg ggtccgctac ggaaaatccg cacgagtgaa
      721 actcggggga ggccttcgga tctctcacgg aaggcgagcg ctccggatca gcgacctcat
      781 ggtcgtctct ccggccggca aaccggcgag gatcgacggc cgcctcggcc ggacccggca
      841 caccttgttc cgggtcaaag ccggcaagcg gatcttcaac gctgcgagcg gcaagctctc
      901 aaaggtcgga tccgcccggc tgaccgccgg cggcgcccgg gtactgaacc gcaacctccg
      961 cctccgccgc agcagactga aggccggact ggtctgggga tacatgaacc tctactcgct
     1021 ctacaaggtg acccaggtcg atgatccgac cggcgaggtt cccgaagtgc cgccggagaa
     1081 ggaacggccg atcgaggcga agaccgttgc ctcggcggcg acagtcaagt ggttcgtccg
     1141 cgacagcttc atcaactatg tcgccagcgg agaaggaacg agagtcgagg acggcgccac
     1201 ggctgacgcg ccgagcggcc cggaccatct cgtctactcg ttcaacttcc ccttcgacag
     1261 cggctggaca gtgcccgagg caatcgacag ccccgagaac accgtgatca ggggaaccgg
     1321 cctggtcggt ttccgctact gccacaacac gatcaacttc accgccgcca gtccggtgat
     1381 cgaaatcgat ggtgacgcca actcccgact gatcttcacc gtccacggaa ccgacgggac
     1441 cgccttcccc gagcagccgg ccgtcatggt caagttgatc ccgagccggg cccagtcaca
     1501 cacggtggtc gacaacatga acggttacag caccgtgacc tgggagaaga tccccggcta
     1561 cgtgccggcc gagggcacgg gcattttcgc cggcttctat ccggccttca gtccggacta
     1621 cgaaggccag ccccagaccg aacgccccga ccggttcggt tacttctcga tcacctacac
     1681 gttcccgaac acggaccatt gagccaggtc gatccgagcc ggacatcgac ttcggggcgg
     1741 atcctcgcct tcggggcggc cctgctggcg acagtcgtcg gctcgatgtc cctggctgct
     1801 caatccccgg ccgcgatcca ggaggggatc gtcgaagtga acggatacgc ctggaccgtg
     1861 aacccggtcg cggtcgacct gaatgacatc gcaccgacca acaacgggag cagcaggtac
     1921 acggtcctcg agatcctcga ggaagggcaa aacggccagt accggatcag tcagatcccc
     1981 cgtgtcacga tcaatctccc cggaaagctg accggacccg gctgctccgg cgaccagatc
     2041 cgcggcaact cgaatcagtg cccgaccttc cggtccaccc ctgacgcaac catcatgagc
     2101 tacaaggccg gctcgcagac caggcaggtc gagtaccggg actacaaccc gcagatctac
     2161 atcgagaaag cggacgacat gtcggtcgac ctttcgccgc ccagcgccaa gatcgacagc
     2221 ggcggccagg tcaggttcac gaccgatgtc agcggggaca ttggcgccgt cacctatgag
     2281 tgggacttcg gcgacgggac caaaaagacg acttcgaaag gctcgatctc ccacaccttc
     2341 aagggcagca accggactta cacggtcgtg gtcagcgtca cttcgaccgg caacccccgc
     2401 agcgccgatg acctgtccac gatcaccgtc ggcaaagtga agaagaagcc ggccgaccag
     2461 aatccgaacc agaagaaccc ggacaacagc gacaacgggg gcggagggga gaccaccgaa
     2521 tcgaccccat acgttcccgg gtattcgggc ggcagcacgg gcggaaccgg cggcggcagc
     2581 cccggggctt cgccgaacac cagccccggt cagccgaagc gggaaaagca gcagccggcg
     2641 aacgaaagca gcggccagac cgtgaccggg cagttgatcg atccggccag catcgccacc
     2701 gcgacccctt cgaccggtga ctcgaccgcg gcgaccgagc aagcgagccc ggacgatggc
     2761 tcgggcggcg ggggcatccc ggacggcgcc aaggctgcgc tcgggatcgg cgccctgctc
     2821 ggcctcggcg ggctggccga agccggcgcc ttcaccgggg ccttccgccg tatccgcttc
     2881 cggctctagc tcgtcgtctc agccgcggct gaggatctgc gaaccgccgg tgatcttgtc
     2941 ttcgccgtcc ggcaggcgga tccaggcctt gttggtgccg tcgtagtcgg gcgcccagag
     3001 gatcagggtc gccgggccga agttgttgac ctcgcaggga tcgtcgccct cttcgtcgag
     3061 accgcgccag acccggatga aggggttgtt ttcccattcc tcgccgaccg tcgatggttc
     3121 gcggtggccg ggatggatgc gggtctcggg cggcagggtc atcagccggt ccatgatcga
     3181 gctcttgagc tccggaaacc cggtctcgcc cggggcaacc gtgccgccga ccgtgccctt
     3241 gaagatcacg tcggcggtga tcacgtcggt gccattgatc agcagggcca tgtggccgtc
     3301 cgaatgtccc ggggtggcga tccacctgat cgtcagtccg cccgagacgg tttcgtcccc
     3361 gtcctcgacg atctggtcaa ccttgaagtc gaccaggtcg gcggtcttct ggctggccac
     3421 gatcggcacc ccgtaacggt cggcgacctc gcgcaggtcg accacgtggt cccagtgttc
     3481 atgggtgagc aggatgtggg tgagggtgat cccttcccgg tcgacccgct ccagcagagg
     3541 ctcgatcact ccgttgccgt cgacc
//
LOCUS       DAOQFI010000104         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12280_length_3558_cov_3.481873, whole genome shotgun sequence.
ACCESSION   DAOQFI010000104 DAOQFI010000000
VERSION     DAOQFI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12280_length_3558_cov_3.481873"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLE93_02755"
     CDS             complement(<1..1101)
                     /locus_tag="PLE93_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH85998.1"
                     /translation="MTLIIGVGRSEAHIYWGDTAGDQISRAENDGTGVDSDFLWWSVG
                     NPTAVAIDGTGIYWANNTDNSVRRANIDGSGETLSAWIPTGITSAWGVAIDSTYAYYT
                     NIAGTSIGRKNRNTGVLDQAFITNTGLQPDSVTVSGSYIYWSHSGNAIGRANIDGTGA
                     DPDFISGLSGVRGIAVNGSYIYWAGDTTNGIGRANLNGTGVNQSFITGAATTDGIAVD
                     SSHIYWTLPGPGRIARANLDGSGANTSFIQNAGNPVAVAVDSGVSPPTLTINSGPSGP
                     TSNRTPSFGFTSSGVTLTCSIDQGTPAWTPCTSPYAPPESLADGDWTFRVHAVSSLGS
                     EADQTRTFTVDGQPPETILDGTPNQFTNESTAY"
     gene            1188..1775
                     /locus_tag="PLE93_02760"
     CDS             1188..1775
                     /locus_tag="PLE93_02760"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNH85999.1"
                     /translation="MSNNNSPEDFPNFLNNPVETVAERLLGCFFERNLGGRRMLARIV
                     ETEAYDEGDAASHSHRGRTARTEVMFGEAGYLYVYFTYGMHYCCNVVTGEAGHGSAVL
                     IRAVEPVEGIDSMEERRGLHGRNLTNGPAKLCQALDIDLRLGGHDLREEPLRLLTGEL
                     LPGESSTCTTRIGISKATELLRRHYIAGNPWVSRH"
     gene            complement(1802..2656)
                     /locus_tag="PLE93_02765"
     CDS             complement(1802..2656)
                     /locus_tag="PLE93_02765"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNH86000.1"
                     /translation="MKHRGSSLLAGLLAIACLATVVMAGPATARFIPGLNRVRADKGE
                     VYRKGCLMGHNRVRSGPCRFGDRDSKKKVVLLGDSHAMQWGPGLIRLARKKQWQVIAL
                     TRASCPAALVHIDYYCDTWRRNSLRRIRLMRPGLVLVASAANSDAYSVFTGGGYLSRA
                     ASEQYLVDGMIRTLRRLRRWSKKTVVMQDQAVTPFSVTACLRINSPGNCGFRASRPRA
                     WSYDFKAARRVKKVPVIDPLPMLCPGGWCRATEGDNLIYRNQGHLAATFTREKYGWLG
                     HRLGDPWR"
     gene            complement(2664..>3558)
                     /locus_tag="PLE93_02770"
     CDS             complement(2664..>3558)
                     /locus_tag="PLE93_02770"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNH86001.1"
                     /translation="ITFRIPSGPSNGSSNKTEPAASQAATSQTLNNQENESADGFVPS
                     FSEVKQDKGPVFGDGCLIHGDEPESGECAYGDTESSRNVVVFGDSHALQWTPALIGIA
                     SQRGWRLTALLHANCTAAQVSVDENCDRWRENSLKRISEEKPGLVIVASNTGPNMTVV
                     SDGTKLDREAAGSSLEAGMARTFEDLKASGAEVTLMQDMAMSKDYLPSECVAGHQDDP
                     GQCTFEMDRPPELAYDLKAARQVGGVQVIDPLPKICPGYSCYPVHGRYLKFRDRFHIT
                     ATYSRLLAPWLDSRLQNPWEE"
BASE COUNT          580 a         1112 c         1223 g          643 t
ORIGIN      
        1 gtaggcggtg gattcgttgg tgaactggtt cggggtaccg tcgaggatcg tttccggcgg
       61 ctgtccgtca accgtgaacg tgcgggtctg gtccgcctca ctgcccagcg agctgaccgc
      121 gtggacccgg aaggtccagt caccgtcggc cagggactcg ggcggggcat aaggcgaggt
      181 gcagggagtc cacgcaggag tgccctgatc aatcgagcag gtgagagtca cgccggagct
      241 ggtgaatccg aaggaaggcg tgcggttgct ggtcggcccc gagggaccgg aattgatcgt
      301 cagggttggc gggcttaccc cgctgtcaac cgcgacggcg acggggttgc cggcgttctg
      361 gatgaagctg gtgttggcac cggacccgtc cagattggcg cgggcgatcc ggcccggacc
      421 cggcaaggtc cagtagatgt ggctggaatc aaccgcgatt ccgtcagttg tcgcggcgcc
      481 ggtgatgaag ctctggttga cgccggtccc gttcaggttg gcacggccga ttccgttcgt
      541 ggtatccccg gcccagtaga tgtagctccc gttgacggcg atcccccgga ctccgctcag
      601 gccgctgatg aaatcggggt cggccccggt gccgtcgatg tttgcgcgac cgatcgcgtt
      661 gccactgtga ctccagtaga tgtaggaccc gctgacggtt accgagtccg gctgcaatcc
      721 ggtgttggtg atgaaggcct ggtcgaggac gccggtgttc cggttctttc gcccgatcga
      781 agtgccggca atgttcgtgt agtacgcgta ggtcgaatcg atcgcgacgc cccaggcgct
      841 ggttatgccg gtcggaatcc aggcgctgag agtttctccg ctgccgtcga tgttcgcccg
      901 acggaccgag ttgtcggtgt tgttcgccca gtagatcccg gtgccgtcga tcgcaacggc
      961 cgtcgggttg ccgaccgacc accagaggaa gtcggagtca acgcccgttc cgtcgttctc
     1021 ggcccgggag atctggtcac ccgccgtatc gccccagtag atgtgagcct cggagcggcc
     1081 gacgccgata attagggtca atgcgaagag cagggcaagc gctcacgcag ccatcgcccc
     1141 catgtgcctg attctgtggg tcaaagcgtc ttgcctctcg agtaggaatg tcgaacaaca
     1201 atagtccaga agatttcccg aacttcctga ataacccggt cgaaaccgtc gcggagcggt
     1261 tgctcggttg ctttttcgag cgaaatctgg gcgggcgccg gatgctggcc cggatcgtcg
     1321 agaccgaggc ctacgacgaa ggggatgccg ccagccactc gcaccggggc aggacggcac
     1381 gaaccgaggt catgttcggc gaggcgggtt acctgtacgt gtacttcacc tacggaatgc
     1441 actactgctg caacgtggtc accggcgagg ccggacacgg gtcggccgtg ctgattcggg
     1501 ccgtggaacc ggtcgagggc atcgactcga tggaggagcg tcgcgggctt cacggccgga
     1561 acctcaccaa cggaccggcc aagctctgcc aggccctcga catcgacctg cggctcggcg
     1621 gtcacgacct gcgcgaggag cccctgcggc tcctgaccgg agagctgctt ccgggtgaat
     1681 cctcgacctg caccaccagg atcgggatca gcaaggcaac cgagctgctt cgccggcact
     1741 acatcgccgg caatccctgg gtctcccgcc actagcggag cggactgccg ctcctgggcg
     1801 gtcagcgcca ggggtcgccg agcctgtggc ccagccagcc gtacttctcg cgggtgaagg
     1861 tggcggcgag gtggccctgg ttgcggtaga tcagattgtc gccctcggtc gcccggcacc
     1921 agccgcccgg gcagagcatc ggcaggggat cgatgaccgg aaccttcttc acccggcgcg
     1981 ccgccttgaa gtcatatgac caggcccgcg gacgcgaagc ccggaaacca caattcccgg
     2041 ggctgttgat gcggaggcag gcggtcacgc tgaacggggt gaccgcctgg tcctgcatca
     2101 cgaccgtctt cttcgaccag cgacgcaggc gccgcagggt gcggatcatc ccgtcgacca
     2161 ggtactgctc gctggccgcg cggctgagat agccaccgcc ggtgaaaacc gaatacgcat
     2221 cgctgttggc ggccgaggca accagcacga ggccggggcg catcaaccgg atccgccgga
     2281 gtgagttcct tcgccaggtg tcgcagtagt agtcgatgtg gaccagcgcc gccgggcagc
     2341 tggctcgggt cagggcgatc acctgccact gcttcttcct ggccagccgg atcagccccg
     2401 ggccccactg catggcgtgg gaatcaccga gcaggacgac cttcttcttc gagtcgcggt
     2461 ccccgaagcg gcaggggccg gaccggaccc ggttgtgacc catcaggcag cccttgcggt
     2521 agacctcgcc cttgtcagcc ctgacccggt tgagaccggg gatgaaacga gcggtggccg
     2581 gaccggccat gacgacggtg gccaggcagg caatcgcgag gagccccgcg agaagtgacg
     2641 acccgcgatg cttcactcgc gggtcactcc tcccagggat tctggagccg cgagtcaagc
     2701 catggcgcga gaaggcgtga ataggtcgcg gtgatgtgga agcggtcgcg gaacttcagg
     2761 taccggccgt ggacggggta gcagctgtag ccggggcaga tcttcggcag ggggtcgatc
     2821 acctggacac cgccgacctg tctggccgcc ttgaggtcat aggcaagctc cggcgggcga
     2881 tccatctcga aggtgcattg gcccgggtcg tcctggtgcc cggcgacgca ttccgaaggt
     2941 aagtagtcct tcgacatcgc catgtcctgc atcagggtca cctcggcgcc gctcgccttg
     3001 aggtcctcga aggtcctggc catcccggcc tcgagcgacg acccggccgc ctcccggtcc
     3061 agtttcgtgc cgtcgctcac gacggtcatg ttggggccgg tgttgctggc cacgatgacg
     3121 agtccgggct tttcctcact gatcctcttc agcgagtttt cccgccagcg gtcgcagttt
     3181 tcgtcgacgc tgacctgggc tgcggtgcag ttcgcatgga gcagggcggt caggcgccag
     3241 ccgcgctggc tggcgatccc gatcagggcc ggcgtccact ggagcgcgtg cgaatccccg
     3301 aagaccacta cgttccggga cgactcggtg tcgccgtagg cacactcacc ggattcgggc
     3361 tcatcgccgt gaatcaggca gccgtcgccg aagaccgggc ccttgtcctg tttgacctcg
     3421 ctgaaggaag gaacgaaccc gtcggcggat tcgttttcct ggttgttcag ggtctggctg
     3481 gttgcggcct ggcttgcggc cggttcggtt ttgttgctgg acccgttcga aggtccggag
     3541 gggatgcgga aggtgatc
//
LOCUS       DAOQFI010000105         3531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12421_length_3531_cov_3.490506, whole genome shotgun sequence.
ACCESSION   DAOQFI010000105 DAOQFI010000000
VERSION     DAOQFI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12421_length_3531_cov_3.490506"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PLE93_02775"
     CDS             <1..1113
                     /locus_tag="PLE93_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNH86002.1"
                     /translation="LYDPVAGTFLADRFVKGTCPKCKAENQYGDSCDRCGATYSPSDL
                     IDPTSTLSGARPESRGAKHLFVELEKLHGFLEEWTQSGKHLQPEVANYLKGHFLNEPL
                     RDWDVSRPAPYFGFEIPDSPGHYWYVWFDAPIGYMASTREWCRRHGENFDDWWRSEQT
                     EIHHFIGKDITYFHTLFWPGMLKSSGFQLPTQVHIHGFLTVGGEKMSKSKGTFVRAET
                     YLKHLDPAYLRYYYASKLGPRLDDLDLNLNEFVQKVNADLVNKVVNLASRSAKLIQDR
                     GLSATGSMTTRTSAPCFGAGSLSPQPVSSPWVSSRGRGGPRSFWRHRCSSPSTSCTSS
                     GPRTTQTGAASPSTPTSPRSGSLTRPSSSRSGLQGC"
     gene            1156..1896
                     /locus_tag="PLE93_02780"
     CDS             1156..1896
                     /locus_tag="PLE93_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86003.1"
                     /translation="MGKYLAGMLLTVFGLFALAPNAGAAEIPPSEECVATAKKLLGEY
                     PDVENFTEEETRKADRKFVNDLADSGCISDSRPLLQKVVLKPNSEKCVTAARGAGAFW
                     GATTKRFRPLNQRWRKQGRPLQRRINRIQRRIRQLREAGASRERLNQLVRIRRPLGRK
                     LQRIQRDLQREARLIVKPMMHESFLVTSELIALRCMPRNLLGESDPGPAERVVRKNID
                     VVLFSLAYLTEATGGEVVFNLYFPTVGR"
     gene            1917..>3531
                     /locus_tag="PLE93_02785"
     CDS             1917..>3531
                     /locus_tag="PLE93_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86004.1"
                     /translation="MGRIRYLLTGFVALAFLGTGFGGTANALTPPAAPSSIAFSFGDR
                     IVRIAADGSGRQSLTRTGPFPDGDLAGRTGDAAPQLSPDGAQLIFTRSLPRKDYGVRR
                     SIMLANADGSGARVILRGNRKVDFSAPAWMPDGKSVAVARSIDRPNGTARSVVIASLN
                     GKSVKTVFTLPLHRRGDLKQDLATYREPIDIGVSPDGKRLLITVSDGYSYDRKWLVLV
                     NLNNGKQRMIGKNTRSGSFSPDGSRFAYVSSTGNSGETCIETDETSCVIQGDLFVQNL
                     DGSGKRRLTRSRAAEENPDWSPDGKRIAFDANYNLPGSGAASEVYAIAPNGTCLSWLT
                     NGSPASVEPDWAPEAGPSDLLCGIEPRETVISSDWIPTKEEGAEISVWAGPVLGNRLV
                     SEAVLTGPFRATVYDDCAVFNARACAEPAKYEIGGLSTCYAGLYLGIVVGEAKRSDFA
                     HRRGVAFLREKRRTRRGNLVRSVTVFTGDTMLAIRGRGNASFADLEKQIDGLRPATAS
                     VSAGRLPQLYLPKGMPNTVKFVAGMVGRIG"
BASE COUNT          655 a         1131 c         1104 g          641 t
ORIGIN      
        1 ctgtacgacc cggtcgccgg cacgttcctc gccgaccgct tcgtcaaagg cacctgcccg
       61 aagtgcaagg ccgagaatca atacggcgac agctgcgacc gctgcggcgc gacctactca
      121 cccagcgacc tgatcgatcc gacgagtacg ctgtcgggcg cccggccgga gtcgcgcggc
      181 gccaagcacc tgttcgtcga actggagaag ctgcacgggt tcctggaaga gtggactcag
      241 agcggcaagc acctgcagcc ggaggtggcc aactacctga agggccactt cttgaacgag
      301 ccgctgcggg actgggacgt gtcgcggccc gcgccgtact tcggcttcga aatccccgat
      361 agtcccggtc actattggta cgtttggttc gacgcgccga tcggatatat ggcgtccact
      421 cgcgagtggt gccgccgcca cggcgagaac ttcgacgact ggtggcgcag cgaacagacc
      481 gagatccacc acttcatcgg caaggacatt acgtatttcc atacactgtt ttggcccggc
      541 atgttgaagt cgtccgggtt ccaattgccg actcaggtcc atattcacgg cttcctgacg
      601 gtcggcggcg agaagatgtc gaagagcaag ggcacgttcg tccgcgccga gacgtacctc
      661 aagcatctcg acccggccta cctgcgctac tactacgcct cgaagctcgg cccgcgcctc
      721 gacgatctcg acctgaactt gaacgagttc gtgcagaagg tgaacgccga tttggtgaac
      781 aaggtcgtga atctggcgag tcgttcagcc aagttgattc aggaccgagg attgtcggcg
      841 accgggtcta tgactacccg tacttcagcc ccatgctttg gggctgggtc gctctcgccg
      901 cagccggttt cttcgccctg ggtttcttct cgaggtcgtg gtggtcctcg ctccttctgg
      961 cggcaccgtt gctcgtcgcc ttctacttcg tgtacgtcgt ctggaccacg aactacccaa
     1021 acgggggcag cctcaccgtc aacaccaact tccccgagat ctggctcgct tactcggcca
     1081 tcttcgtccc ggtctgggct gcaggggtgt tgattgcaaa gggccggggt cgagggtcga
     1141 aatagaatga agcgcatggg gaaatacctg gcagggatgc tgttgacggt tttcgggctg
     1201 ttcgcgcttg ctccgaacgc gggagcggcc gaaattccgc cgagcgagga gtgcgtcgcc
     1261 accgccaaga agctgctcgg cgagtatccg gacgtcgaga atttcaccga ggaggagacc
     1321 agaaaggccg accggaagtt cgtaaacgat ctcgccgatt caggctgcat ttcagattcc
     1381 aggccactcc ttcagaaggt tgtgctcaag ccgaacagcg agaagtgcgt aacggctgcg
     1441 cgaggggccg gagcgttctg gggagcaacc acgaagaggt tccgaccgct caaccagagg
     1501 tggcggaaac agggccgtcc cctccagcga cgcatcaacc gaattcagcg gcggatccgc
     1561 cagctccggg aagccggggc ctcccgggag cgactcaatc agctggttcg aatccggaga
     1621 cctctcggca ggaagctcca aaggatccag agagatctcc agcgggaagc ccgcctgatc
     1681 gtgaagccga tgatgcatga gtccttcctc gtcaccagcg aactgatcgc cctccgctgc
     1741 atgccccgga accttctcgg tgaaagcgat ccggggccgg cggaaagggt ggtcaggaag
     1801 aacatcgacg tggtcctgtt ttccctggcc tacctgaccg aagcgaccgg cggcgaggtc
     1861 gtgttcaacc tctacttccc gaccgtaggt cgctgaagca gaggggaagg ggaaggatgg
     1921 gccggattcg gtacttgctc accggtttcg tcgcgctggc atttctcgga accggatttg
     1981 gcggtaccgc caatgcgctg actccgcccg ctgctccgag cagcatcgct ttcagcttcg
     2041 gtgaccggat cgttcgcatc gccgccgatg gttcgggacg gcagtcgctg accaggaccg
     2101 gccccttccc ggatggcgac ctcgcaggcc gtaccggtga cgctgctccc cagctttcgc
     2161 ccgatggcgc ccagctcatt ttcacccggt ctcttccgcg aaaggactac ggcgtccgca
     2221 ggtcgatcat gctggccaat gccgatggca gcggagcccg cgtgatcctt cgcggcaacc
     2281 gcaaggtcga cttttccgcc ccagcctgga tgcccgacgg caagtccgtc gcggtggctc
     2341 gcagcatcga ccggcccaac ggcacagcgc gctcggtcgt gatcgccagc ctcaacggca
     2401 agtcggtgaa aacggtcttc acgctgccgc tccaccgccg gggagacctg aagcaggacc
     2461 tcgccaccta ccgcgaaccg atcgacatcg gtgtttcccc cgacggaaag cggttgctga
     2521 tcacggtgag cgacggctac agctacgacc ggaagtggct cgtgctggtc aacctgaaca
     2581 acggcaagca gcgcatgatc ggcaagaaca cgcggtccgg ctccttctca cccgacggga
     2641 gccgcttcgc ctatgtctcg tcgacgggca acagcggtga aacctgcatc gaaaccgatg
     2701 aaacgagctg cgtcatccag ggtgacctgt ttgttcagaa cctcgacggc agcggcaagc
     2761 ggaggctgac ccggagtcgc gccgcggagg aaaacccgga ctggtccccc gacggcaagc
     2821 ggatcgcttt cgacgccaac tacaacctcc ccggatcggg cgcggcctcc gaggtctacg
     2881 cgatcgctcc gaacgggacc tgcctttcgt ggctcaccaa cggttcgccg gcgtcagtcg
     2941 aaccggactg ggctcccgaa gccggccctt cggatctcct ttgcggaatc gagccaaggg
     3001 aaacggtgat ttccagtgac tggatcccca ccaaggagga gggtgccgag atcagcgtct
     3061 gggccggtcc cgtgctgggc aatcgcctgg tctccgaggc cgtcctgacg ggtcccttcc
     3121 gcgccactgt gtacgacgat tgcgcggtct tcaacgccag ggcctgcgcc gagcctgcga
     3181 agtatgaaat cggcgggctt tccacctgct acgcgggtct ttacctcggc atcgtggtcg
     3241 gcgaagcgaa gcgatccgac ttcgcccatc gccggggcgt cgccttcctt cgtgaaaagc
     3301 ggcgcactag gcgcgggaat ctggtccgta gcgtcacggt cttcaccggt gacaccatgc
     3361 ttgcgattcg cggccgaggg aacgccagct tcgccgatct cgagaagcag atcgacgggc
     3421 ttcgaccggc caccgcttcc gtgtcggccg ggcgtttgcc gcagctctac ctgccgaagg
     3481 gaatgccgaa cacggtcaag tttgtggccg ggatggtcgg ccggatcgga g
//
LOCUS       DAOQFI010000106         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12448_length_3527_cov_3.110023, whole genome shotgun sequence.
ACCESSION   DAOQFI010000106 DAOQFI010000000
VERSION     DAOQFI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12448_length_3527_cov_3.110023"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1451)
                     /gene="nuoD"
                     /locus_tag="PLE93_02790"
     CDS             complement(129..1451)
                     /gene="nuoD"
                     /locus_tag="PLE93_02790"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNH86005.1"
                     /translation="MARVEDTLHDREIRQAGAIGEAGTLRDEEITVSDFTTELAERIP
                     GVEFELEPEQEMLTVNMGPHHPATHGVLRLVVDLEGEVVKDLKPVIGYVHTGIEKSCE
                     TMQYWKVIPFVERMDYLSYFFNMQAYVGAVEKLIGLEVPRRAQWLRVLHMELNRIHSH
                     LVWLGTSALDLGAMGVYFYCFRDRDRVLDTFEMSTGQRMHTRYFQIGGVAEDIPAGFE
                     QKVRKTIEQLKTGIGQYEALLDKNEIFLIRTKDVGVVSRERLVELGVTGPLLRAAGDP
                     WDLRKEGGYLAYPELEFDVPVGTVGDGYDRYRVRLTEMRESIKIIEQILDGLHEGPWI
                     ADDRKYALPPRAELSTSMESLIHHFKLVTEGFRVEPGEVYNPIESPRGELGCFVMADG
                     SSKPARVHFREPSFVNLQALADMTIGEYVADLIQTLAMLDPILGGIDR"
     gene            complement(1451..2110)
                     /locus_tag="PLE93_02795"
     CDS             complement(1451..2110)
                     /locus_tag="PLE93_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNH86006.1"
                     /translation="MPDAPGLELILAEINREHPGAVLDTSYDREQAALIIDPSKVIEV
                     LTWLKQTPGQEYTFLSSVHGADHFPLTPRFAVHYELLNRERYERLRVKAVIPDPGAAP
                     SEVSVVAPGSSDAGAVDSDSGERGSSMEMPETEGRSTVLDGETLPVVPSVVELFPTAD
                     FQEREVYDFFGIVFEGHPDLRRILMPEDYVGWPQRRDFPVGGEPVIFTRDEVTNPGWW
                     Q"
     gene            complement(2103..2717)
                     /locus_tag="PLE93_02800"
     CDS             complement(2103..2717)
                     /locus_tag="PLE93_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNH86007.1"
                     /translation="MLRGDLTGEDLDKYVEERVVTTTLEKVLNQARAHSVFPATFGLA
                     CCAIEMMSTVSPRYDIARFGAEAFRASPRQADLLILSGRVSIKMAPVVRRIYDQMLEP
                     KWAISMGACSSSGGMFQNYAVVQGADKFMPVDVHVPGCPPRPEALLYGFTKLQQKIMG
                     NPDPGWRSRYNAHGTEEWARGGPASRPSEEALEAYAKAREVLGA"
     gene            complement(2804..3184)
                     /locus_tag="PLE93_02805"
     CDS             complement(2804..3184)
                     /locus_tag="PLE93_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNH86008.1"
                     /translation="MLHQYLPVLVFGALGIIVGGAFATLNLLLGPKKVKGDESSAAVR
                     QLDPYECGLPSEISKTFRFGVSFYLVAMLFILFDIEVVFLYPVGAILKSTDSVFVLAE
                     VILFVALLFVALIYVWRKGALDWR"
     gene            3337..>3527
                     /locus_tag="PLE93_02810"
     CDS             3337..>3527
                     /locus_tag="PLE93_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86009.1"
                     /translation="MSAAYTAAGLAVIALNLLAAVVGAVSWQRNVPSVAFWYLLRAAQ
                     IATGFFVLFACVVYATGYRA"
BASE COUNT          607 a         1097 c         1186 g          637 t
ORIGIN      
        1 cggaatctgt tccgggtcac cgaccgggag gcgtccggtg gtgttcgccc gatcgaccgg
       61 caggccgggc gagacgaaat cctccggctc ggcatcgcgg gggtcgaaat ccgacggcat
      121 cacgggggtc atcggtcgat acctcccagg atcgggtcga gcatggccag ggtctggatc
      181 aggtcggcca cgtattcgcc gatcgtcatg tcggccaggg cctgaaggtt gacgaaggaa
      241 ggctcgcgga aatggacccg ggcgggtttc gacgagccgt cggccatcac gaagcagccc
      301 agttcgccgc ggggcgattc aatcgggttg tagacctcgc ccggctcgac ccggaagcct
      361 tcggtgacca gcttgaagtg gtggatcagc gattccatcg aggtcgaaag ctcggcccgg
      421 ggcggcagcg cgtacttgcg gtcatcggcg atccaggggc cctcgtgcaa cccgtcgagg
      481 atctgctcga tgatcttgat cgactcgcgc atctcggtca ggcggacccg gtaccggtcg
      541 tagccatcac cgaccgtgcc gaccggcacg tcgaattcga gttccggata ggcgaggtag
      601 ccgccttcct tgcggaggtc ccaggggtcg ccggcagcgc gcagcagcgg cccggtcacg
      661 cccagctcga ccagtcgctc gcgcgagacg acgccgacgt ccttggtccg gatcaggaag
      721 atctcgttct tgtcgagtag cgcttcgtac tggccgatgc cggtcttcag ctgttcgatc
      781 gtctttcgca ccttctgctc gaaaccggcc gggatgtcct cggctacccc gccgatctgg
      841 aagtagcggg tatgcatgcg ctggccggtc gacatctcga aggtgtcaag tacccggtcg
      901 cggtcacgga agcaatagaa gtagacgccc atcgcaccga ggtcgagagc cgaggtgccg
      961 agccagacca gatgcgagtg aatccggttc agctccatgt ggagcacccg cagccattgc
     1021 gcgcggcggg gaacctcgag gccgatcagc ttctcgaccg cccccacgta ggcctgcatg
     1081 ttgaagaagt aggagaggta gtccatccgc tcgacgaagg ggatgacctt ccagtactgc
     1141 atggtctcgc aggacttctc gatgccggtg tggacgtagc cgatcaccgg cttcaggtcc
     1201 ttgaccacct cgccctcgag atcgaccacg agccgcagca cgccgtgggt cgcggggtga
     1261 tgcgggccca tgttgaccgt gagcatctcc tgttccggtt ccagttcgaa ctcgactccg
     1321 gggatccgct cggccagttc ggtcgtgaag tcggagaccg tgatctcttc gtcgcgcagg
     1381 gtgccggcct cgccgatcgc cccggcctgc cggatttcac ggtcgtgaag tgtgtcttcg
     1441 acgcgcgcca tcactgccac cagcccgggt tggtcacttc gtcacgggtg aagatcaccg
     1501 gctcgccacc gaccggaaag tcccggcgct gcggccagcc gacgtagtcc tcgggcatca
     1561 ggatccggcg caggtcggga tggccttcga agacgatgcc aaagaagtca tagacctccc
     1621 gctcctggaa atcggccgtc gggaagagct cgaccacgga cggcacgacc gggagagtct
     1681 ctccatcgag gaccgtcgag cgaccttccg tttctggcat ctccatcgat gagccccgtt
     1741 caccggaatc ggaatcgacc gccccggcgt cgctgctccc gggcgcgacg accgagactt
     1801 cgctgggcgc ggccccgggg tccgggatga cggccttcac gcggaggcgt tcgtagcgtt
     1861 cgcggttgag gagttcgtag tggaccgcga agcgcggagt gagggggaaa tggtcggcgc
     1921 cgtggaccga gctgaggaag gtgtactcct ggcccggcgt ctgcttgagc caggtcagga
     1981 cctcgatcac cttcgagggg tcgatgatca acgccgcctg ctcccggtcg tagctcgtgt
     2041 cgagcacggc cccgggatgt tcgcggttga tctcggcgag gatgagttcc agtccgggcg
     2101 cgtcaggcac cgaggacctc ccgggccttc gcgtaggcct cgagtgcttc ctccgaaggt
     2161 cgtgaagccg ggccgccgcg ggcccactcc tcggttccgt gggcgttgta gcgactgcgc
     2221 cagcccggat cgggattgcc catgatcttc tgctgaagct tggtgaaccc gtagagcagt
     2281 gcctcgggcc ggggcgggca gccgggcacg tggacgtcga ccggcatgaa cttgtcggct
     2341 ccctgaacca ccgcgtagtt ctggaacatc ccgccggagg aggagcaggc gcccatcgag
     2401 attgcccact tcggttccag catctggtcg tagatgcggc ggacgaccgg cgccatcttg
     2461 atcgagaccc ggccggaaag gatcaacagg tcagcctggc ggggcgaggc ccggaaggct
     2521 tcggcaccga aacgggcgat gtcatagcgc ggtgaaacgg tcgacatcat ctcgatcgcg
     2581 cagcaggcga gaccgaaggt cgcggggaag accgaatggg cacgggcctg gttgaggacc
     2641 ttctcgaggg tggtcgtgac gactctctcc tcgacgtact tgtcgaggtc ttcgccggtc
     2701 aggtcgccgc gcagcatgtc gcgggccttg agctggcggg cgcggagctc ttccggtgac
     2761 ttgggctgct cgaggggggc gtgaacccgg agcttctggc gttctatctc caatccaggg
     2821 ctcccttccg ccagacgtag atcagtgcca cgaagagcag ggccacaaag aggatcactt
     2881 cggccaggac gaagaccgag tccgtcgact tgaggatcgc ccccaccggg tagaggaaga
     2941 cgacttcgat gtcgaacagg atgaagagca tcgccaccag gtagaagctg accccgaagc
     3001 ggaaggtctt cgagatctcc gaaggcaggc cgcactcgta gggatccagc tggcggaccg
     3061 cagcgctgga ctcgtccccc ttcaccttct tcggccccag cagcaggttc agggtcgcga
     3121 aagcaccacc gacgatgatg ccgagggcgc cgaagacgag gactgggagg tactgatgga
     3181 gcattggtgg gaggtgggtg gtcggttcgt cagggaacta aggtgtccgc tgtctatggg
     3241 ttatacacct ccgcgccccg actttgtatc aagtcgttac atagtgattt ttcgcataaa
     3301 aaagcggaat cccgaccata tggggcggga ttagcagtgt ctgccgccta taccgctgcg
     3361 ggtctcgccg tgattgcgct caacctgctc gccgcggttg tcggggctgt ctcctggcaa
     3421 aggaacgttc catcggtcgc cttctggtac ctgctgcggg ccgcccagat cgccaccggc
     3481 ttcttcgtcc tcttcgcctg cgtcgtctac gcgacgggct accgggc
//
LOCUS       DAOQFI010000107         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12538_length_3510_cov_2.667728, whole genome shotgun sequence.
ACCESSION   DAOQFI010000107 DAOQFI010000000
VERSION     DAOQFI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12538_length_3510_cov_2.667728"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /gene="rplT"
                     /locus_tag="PLE93_02815"
     CDS             <1..236
                     /gene="rplT"
                     /locus_tag="PLE93_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNH86010.1"
                     /translation="GAYAYRDRRVRKREFRRLWITRINAAARLEGMSYSELMHGLSAA
                     GVEVNRKMLADIAVNDREDFRRFVEIAREGAAA"
     gene            330..1052
                     /locus_tag="PLE93_02820"
     CDS             330..1052
                     /locus_tag="PLE93_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNH86011.1"
                     /translation="MIVSKENEKLKLVRKLGQKKHRRKLGMFVTEGEDLARAGLAAGH
                     EPVAMLVHPETDLPGEKVEPDLLDGVAALASGTRVIGIWSEIWAEPELLTADKCIFLD
                     GVADPGNVGTIIRTADALVGGTVVLGPGSADPFSGIAVRSSMGSIFTQPVVKAEIGQT
                     PAPRIATVPQGGERPGPVEGPVTLCLGSEREGLSPEILKECGITWTIPQRSGGAESLN
                     VAAAAAIACERISSQPGESAQD"
     gene            1081..2148
                     /gene="pheS"
                     /locus_tag="PLE93_02825"
     CDS             1081..2148
                     /gene="pheS"
                     /locus_tag="PLE93_02825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNH86012.1"
                     /translation="MVERIEEIEVEAQAAVEAADSAAELEEVRVRYLGRKAELTGMLR
                     GIADLPPEQRGPVGSTGNKVRKGLEGLIEAKVEALRNAELEQSLAGDRIDVTLPGSPA
                     RAVGHLHPITRTRRLIEDVMIGLGYQVMEGPEVEHDYYNFTALNHPEGHPARMLQDTF
                     YVDTDEHVEQVLLRTHTSPMQVRAMESAPPPLFMIVPGKTYRRDSDATHSPMFHQVEG
                     LAVGEGITLADLKGTLLAMLREIFGPDREIRMRPHFFPFTEPSVEFDVSCFRCGGSGS
                     LEDGSRCGLCKGIGWIELGGAGMVDPNVFGFVADSGYDPDSVTGFAFGFGIERIAMLQ
                     HNVGDLRLFFDNDLRMLEQFS"
     gene            2145..>3510
                     /gene="pheT"
                     /locus_tag="PLE93_02830"
     CDS             2145..>3510
                     /gene="pheT"
                     /locus_tag="PLE93_02830"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNH86013.1"
                     /translation="MRVPYGWMADYCDPGLEPRDLAEKMAMTGTEVERVVTLGPPSAE
                     GFVIGKVLAVEKHPDADRLNVCQVDTGGETRTIVCGAPNVAAGQTVVVALPGAVMPGG
                     MEIKQAKLRGVESNGMICSERELELGEGHEGILVLGDGRAPGTPAGQLLPLAEPVLEL
                     EVTPNRTDCFGIYGVAREAHAISSAPLAPAPWDGGPIGPGLNSEPGDLERLDDPVGDQ
                     KTADRVRILVQDEDLCPRFTARGYSEVKVGPSPTWLKARLIAAGMRPISNVVDITNYV
                     MWLTGQPMHAYDLDLVPEGELIVRRAENGEKLAALDGSEYELTSEMVAVADRGGLAGI
                     GGMMGGSVSEVSESTTTVLMESANWNGPNILKTSRDLGLRSEASSRFEKQLHPDLTLR
                     AQAVAARLMVEHGVDDFLFAKRKAAERLGVGEHAVLPKNSEIEAAIVEYQQLFLGEEH
                     VETLA"
BASE COUNT          632 a         1139 c         1202 g          537 t
ORIGIN      
        1 cgggcgccta cgcctaccgt gaccgccggg tccgcaagcg ggaattccgt cgcctctgga
       61 tcacccgcat caacgcagct gcccgcctcg agggcatgag ctactccgag ctgatgcatg
      121 gcctcagcgc cgccggcgtc gaggtcaacc gcaagatgct cgccgacatc gcggtcaacg
      181 accgtgagga ctttcgccga tttgtcgaga tcgcccggga gggtgccgcc gcctagtgcg
      241 ctgacggcac ctcgaaccca ccgggcggct tcgcgatctg cgagccgccc tttttgtttt
      301 cgggggccgg aaagagaaca gaccgaacca tgatcgtcag caaggaaaac gaaaaactga
      361 aactggtccg caagctgggc cagaagaagc accgccggaa gctgggaatg ttcgtcaccg
      421 agggtgaaga cctggcccgg gcgggtctgg ccgcaggcca tgagcccgtg gcgatgctgg
      481 tacatcccga gaccgacctt ccgggggaaa aagtcgaacc ggacctgctg gacggggttg
      541 ccgcgctcgc ctccggcacc cgggtgatcg ggatctggtc cgagatctgg gccgaaccgg
      601 agctgctgac cgccgacaag tgcatcttcc tcgacggagt cgcggacccg ggcaacgtcg
      661 gcacgatcat ccggaccgcc gacgccctgg tcggcggcac ggtggtgctc gggccgggca
      721 gcgccgaccc cttctccggg atcgcggtgc gctccagcat gggttcgatc ttcacccagc
      781 cggtggtgaa ggcggagatc gggcagaccc cggccccgcg gatcgccacg gtcccgcagg
      841 gaggggagcg gccgggaccg gtcgaaggtc cggtgactct ctgccttggg tccgagcgcg
      901 aagggctttc gccggaaatc ctgaaggagt gcgggatcac ctggacgatc ccgcagcggt
      961 ccggcggagc cgaatcgcta aacgttgcgg ccgcggcggc gatcgcctgc gagcggatat
     1021 cttcgcaacc aggcgaatcc gcacaagact gaggaaaaag gaagctgaac cggggaagaa
     1081 atggttgaac ggatagagga aatcgaagtc gaggcacagg cggccgtcga agccgcagat
     1141 tcggcggccg agctcgaaga ggtccgggtc cgttacctgg gccgcaaggc cgagctgacc
     1201 ggcatgctgc ggggtatcgc cgacctgcca ccggagcagc gcggcccggt cggcagcacc
     1261 ggcaacaagg tccggaaggg cctcgagggc ctgatcgaag cgaaggtcga agctctcagg
     1321 aacgccgaac tggagcagtc cctggccggg gaccggatcg acgtgaccct gccgggcagt
     1381 ccggcccggg cggtcggcca cctgcatccg atcacccgta cacggcggct gatcgaggat
     1441 gtgatgatcg gcctcggcta ccaggtgatg gaaggcccgg aagtcgagca cgactactac
     1501 aacttcaccg cgctcaacca tcccgaaggc catcctgccc ggatgctcca ggacaccttc
     1561 tacgtcgaca ccgacgagca cgtcgagcag gttctgctgc ggacccacac ctcacccatg
     1621 caggtccggg ccatggagtc ggcgccgccg ccgctcttca tgatcgtgcc gggcaagacc
     1681 taccggcgcg attccgatgc cacccacagc ccgatgtttc accaggtcga gggcctggcg
     1741 gtcggcgagg ggatcaccct ggccgacctc aagggaaccc tgctggcgat gctccgcgaa
     1801 atcttcggtc ccgaccggga aatccgcatg cgcccgcact tcttcccctt caccgaaccc
     1861 tcggtggaat tcgacgtctc ctgcttccgc tgtggcggct cggggtcgct cgaagacggt
     1921 tcccgctgcg gtctctgcaa ggggatcggc tggattgaac tgggcggggc cgggatggtt
     1981 gatccgaacg tgttcggatt cgtggccgac tccggctacg accccgactc ggtgaccgga
     2041 ttcgccttcg gtttcggaat tgagcgaatc gcgatgctgc agcacaacgt cggtgacctg
     2101 cggctcttct ttgacaacga cctgcgaatg ctggagcagt tctcatgaga gtcccttacg
     2161 gatggatggc cgactactgc gaccccgggc tcgaaccgcg ggacctcgcc gaaaagatgg
     2221 cgatgaccgg aaccgaggtc gaaagggtgg tcaccctggg gccgccttca gccgaaggct
     2281 tcgtgatcgg caaggtgctc gcggtcgaaa agcacccgga tgccgaccgg ctgaacgtct
     2341 gtcaggtgga caccggcggc gagaccagga cgatcgtctg cggcgccccg aacgtggccg
     2401 ccggccagac cgtggtggtc gccctgcccg gggcggtgat gcccggcggc atggagatca
     2461 agcaggcgaa actccggggg gtcgaatcga acggaatgat ctgctcggaa cgggagctgg
     2521 aactcggcga gggccatgaa ggcatcctgg tgctcggtga cgggcgggcc ccgggcaccc
     2581 cggccggcca gctcctgcca ctggccgagc cggtgctgga acttgaagtc accccgaacc
     2641 ggaccgactg cttcggcatc tacggggtcg cccgtgaagc ccacgcgatc agctcggcgc
     2701 cgctggcgcc agcgccctgg gatggcggcc cgatcggccc gggactgaat tccgaacccg
     2761 gcgacctgga gcgtctggat gatcccgtcg gcgaccagaa gactgccgac cgggtccgga
     2821 tcctggttca ggacgaggac ctctgcccgc gcttcaccgc tcgcggatac agcgaggtga
     2881 aggttggccc ctcaccgacc tggctcaagg cgaggctgat cgctgcgggc atgcggccga
     2941 tctcgaatgt ggtcgacatc accaactacg tgatgtggct gaccggccag ccgatgcatg
     3001 cctacgacct cgacctggtc ccggagggcg agctgatcgt ccggcgggcc gagaacggcg
     3061 agaagctggc cgcgctggac ggcagcgagt atgaactgac ctcggagatg gttgccgtcg
     3121 ccgaccgggg cggtcttgcc ggaatcggcg gcatgatggg cggctcggtc tccgaggtgt
     3181 cggaatccac gactacggtg ctgatggagt cggccaactg gaacggcccg aacatcctca
     3241 agacctcgcg cgacctcggg ctccgttcgg aggcttcctc gcgcttcgaa aagcagcttc
     3301 atcccgacct gaccctgcgg gcccaggccg tggccgcccg gctgatggtc gagcacggtg
     3361 tcgacgactt cctgttcgcg aagcgcaagg ccgcggagcg tctcggcgtc ggcgagcacg
     3421 cggtgctgcc gaagaattcc gagatcgaag ccgcgatcgt cgaataccag cagctgttcc
     3481 tcggcgaaga gcacgtcgag acgctcgccg
//
LOCUS       DAOQFI010000108         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12638_length_3494_cov_2.788311, whole genome shotgun sequence.
ACCESSION   DAOQFI010000108 DAOQFI010000000
VERSION     DAOQFI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12638_length_3494_cov_2.788311"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1595
                     /locus_tag="PLE93_02835"
     CDS             120..1595
                     /locus_tag="PLE93_02835"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HNH86014.1"
                     /translation="MIKVPETKKGKRLTLAGIAAAALVIFILYREFFPELDLQQLLDD
                     FANFLGAWTYLVVGLLAFLETGAFVGLIVPGETAMLIGGAVAGQGVINVFLLIAIAWL
                     MAFLGDSTSFWLGHRLGREFILKHGPKFGISAERYEQVEDYFEKHGGKTVLMGRFIGL
                     VRALSPFVAGSSGMRYRAFAPYSILGSGLQITLHILAGYFFARSIEAAAEYVGLAALV
                     IGTMIVVSVASYLAWKYLREPNNRARAVEWMEGRWYSAWLVRLARRYETQLRWIQDRF
                     TPGGTFGLEVTTLFAIIAVASFVLFAYAGILLDDGGPTPGDLRAFDFRDLIETGWLTS
                     IAKVVTVLGSQVVLLPLITVTAVILAVRQHWSEVLVLVLSSIAIAFGIDWIKDWVARP
                     RPDGGMIEVAGYAYPSGHAAHSIFYVWLSVTIAVRLRPDMARKAALITGGILLAALVG
                     LSRVYLGVHYLSDVFGGWALGALCFSFFAVWALLLGQLRKN"
     gene            1600..1848
                     /locus_tag="PLE93_02840"
     CDS             1600..1848
                     /locus_tag="PLE93_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86015.1"
                     /translation="MVLAVSTQIWYFGAAGLVSLAAFVGLILVPAVSSYGRWHERFAA
                     GFLSLLIFGGLITIGVIAGLAWVLISYGIAPLNPFTGG"
     gene            1845..3011
                     /locus_tag="PLE93_02845"
     CDS             1845..3011
                     /locus_tag="PLE93_02845"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH86016.1"
                     /translation="MSPAPEAKSSAPARLASPVLAGRGLPGLARAAADVLGMPVAFLD
                     RSGIVLAVAGATQAQEAKLTAKEKGVATIELILGDTTVGEARYLGDGVDGDTLEVMSA
                     LAALEVERARSDEWGNEGAVSEFIRAMLDGSMTNTDDIVARAGEVGCDLSRGGGVLMI
                     RAHAGSAQEGDWRSRVLDMALRTIRAIASGAIAGMGESEEGAVVGILVPTNEEDRLVK
                     VEDALDRELPRSLSGLTVTISRSRLATGPDQIERAAREAQLALNVGEAEGRSPIAFED
                     TGAYRLLLGTSNEELMSFYSETVEPLVAYDEQYETDLVATVEAYLDSDANVPATAKQM
                     FTHRHTIRYRLERIRDLSGHDATATEGRERLGLGIKAMRVLGIASPRGRSKERT"
     gene            complement(3143..>3494)
                     /locus_tag="PLE93_02850"
     CDS             complement(3143..>3494)
                     /locus_tag="PLE93_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86017.1"
                     /translation="FNVSRATIRSAIGVLAERLEQQIGECLEQGELPRGAERERLIAV
                     ADALCRATPSSDDSLRKTATVKLNLTARARKLFKDSKKGKRRIKGLKSLKATVLINGK
                     SAGKITVKRTGKVN"
BASE COUNT          504 a         1199 c         1142 g          649 t
ORIGIN      
        1 cggcaccggc cccaccgtgt tcgggctttt ccccgaccgg gaatcggccg aggcggcggc
       61 cgccgccgtt ggtccggagg cgctcgtctg ccagggcggc gtggtctgat ttcgcgctca
      121 tgatcaaggt cccggagacg aaaaagggca aacggctgac cctcgccggc atcgcggcgg
      181 cggccctggt catcttcatc ctctaccggg agttcttccc cgagctcgac ctccagcagc
      241 tgctggacga cttcgcgaac ttcctcggtg cctggaccta cctcgtcgtc ggcctgctcg
      301 ccttccttga aaccggcgcc ttcgtcggcc tgatcgtccc cggagaaacg gcgatgctga
      361 tcggcggtgc ggtcgccggt cagggggtga tcaacgtctt cctgctgatc gcgattgcct
      421 ggctgatggc cttcctcggt gattcgacca gcttctggct cggccaccgg cttggccgcg
      481 aattcatcct caaacacggc ccgaagttcg ggatctcggc ggaacgctac gaacaggtcg
      541 aggactactt cgaaaagcat ggcggcaaga ccgtcctgat gggccggttc atcgggctgg
      601 tccgggccct ttccccgttt gtggcgggca gctccggcat gcggtaccgg gccttcgcgc
      661 cctacagcat cctcggctcc ggcctccaga tcaccctgca catcctggcc ggctatttct
      721 tcgcccgcag catcgaagcg gcggccgagt acgtcgggct ggcggcgctc gtcatcggca
      781 cgatgatcgt ggtctcggtc gcctcctatc tcgcctggaa atacctgcgc gaaccgaata
      841 accgggccag ggcggtcgag tggatggaag gccgctggta cagcgcctgg ctggtgcgac
      901 tggcccgtcg ctacgagacg cagttgcgct ggatccagga ccgcttcacg ccgggcggga
      961 ctttcggtct cgaggtgacg acgctcttcg cgatcatcgc cgtggcctca ttcgtgctct
     1021 tcgcctacgc cgggattctg ctcgacgacg gtggcccgac ccccggcgac ctgcgggcct
     1081 tcgatttcag ggacctgatc gagaccggct ggctgacctc catcgccaag gtagtcacgg
     1141 tgctcggctc ccaggtggtg ctgctgcccc tgatcaccgt gaccgcggtg atactggcgg
     1201 tccggcagca ctggtcggaa gtgctggtgc tggtcctttc ctcgattgcg atcgcgttcg
     1261 gaatcgactg gatcaaggac tgggtcgcgc ggccccgccc cgatggcggg atgatcgagg
     1321 tcgccggcta cgcctacccc tccggacacg ccgcccattc gatcttctat gtctggctct
     1381 cggtcaccat cgccgtccgg ctccgccccg acatggcccg caaggctgcc ctgatcaccg
     1441 ggggcattct gctggccgcg ctggtcggcc tgagccgggt ctatctcggt gtccactacc
     1501 tgagcgacgt cttcggaggc tgggctctcg gggcactctg cttctcgttt ttcgcggtct
     1561 gggccctgct tctgggccag ttgcgcaaaa attaggccga tggtgctcgc cgtttcaacc
     1621 cagatctggt atttcggcgc cgccggcctc gtgagtctcg ccgcattcgt cggcctgatc
     1681 ctggtcccgg cggtgagttc gtacgggcgc tggcacgagc gtttcgcggc cggtttcctc
     1741 tccctgctga tcttcggggg gctgatcacg atcggcgtca tcgcgggact ggcgtgggta
     1801 ctgatctcgt acggcatcgc gccgctcaac cccttcacgg gcgggtgagc cccgccccgg
     1861 aagccaagtc ctcggcgccc gcgcgcctgg ccagtccggt ccttgcgggt cgtggattgc
     1921 cgggcctggc ccgggccgcg gccgacgtgc tgggaatgcc ggtggccttt ctcgaccgtt
     1981 ccgggatcgt gctggccgtg gcgggagcca cccaggcgca ggaagcaaag ctgacggcca
     2041 aggagaaggg cgtcgccacg atcgaattga tcctcggtga caccacggtc ggtgaagccc
     2101 gttacctcgg agacggcgtt gacggcgaca ccctcgaagt gatgtcggcc ctggccgccc
     2161 tggaagtcga gagggcccgt tccgacgaat ggggcaacga gggcgccgtg tcggaattca
     2221 tccgcgcgat gctggacggc tcgatgacca acaccgatga catcgtcgcc cgggccgggg
     2281 aggtcggatg cgacctttcc cgcggcggcg gagtcctgat gatccgggct cacgccggct
     2341 cggcccagga aggtgactgg cgctcccggg tgcttgacat ggccctgcgc acgatccggg
     2401 cgatcgcctc cggcgcgatc gccggcatgg gtgaatccga ggaaggggcc gtggtcggca
     2461 tcctggtccc gaccaacgag gaagaccgcc tggtcaaggt cgaggacgcg ctcgaccggg
     2521 aacttccgcg ctcgctctcg ggcctgaccg taaccatttc ccgctcgcgc ctggccaccg
     2581 ggccggatca gatcgagcgg gccgcccggg aagcccagct cgcgctcaac gtgggcgagg
     2641 ccgagggccg ctccccgatc gccttcgagg acacgggcgc ctaccgcctg ctgctcggca
     2701 ccagcaacga ggaactgatg agcttctatt cggagacggt cgagccactg gtcgcctacg
     2761 acgagcagta tgaaaccgac ctggtcgcca cggtcgaggc ctatctggac agcgatgcga
     2821 acgttcccgc caccgccaag cagatgttca cccaccggca cacgatccgc taccgcctcg
     2881 aacgcatccg cgacctctcc ggccacgacg ccaccgccac cgaaggccgg gaacgcctcg
     2941 gcctcggaat caaggcgatg cgcgtcctcg gcatcgcctc accccggggc cgctccaagg
     3001 aacgaaccta gcccgggcaa cgcacccaga tccggtctcg accggttccg gccctgattc
     3061 gggcaggtgc cgcaggtttg caaccggaga ggtcgcaaac ctgcggcacc gacccaggga
     3121 gactcctccg ggccggttgc ggctagttga ctttgccggt cctcttgacg gtgatcttgc
     3181 cggcggactt gccgttgatc aggacggtgg ccttgagtga cttgaggccc ttgatcctgc
     3241 gcttgccctt cttgctgtct ttgaacagct ttcgcgccct ggcggtcagg ttgagcttga
     3301 ccgtcgcggt cttgcggagg ctatcgtcgg aactgggcgt cgcgcggcag agcgcgtccg
     3361 ccacggcaat cagccgttcc cgttccgcgc cccggggtaa ttcaccctgt tccaggcact
     3421 cgccgatctg ttgctccaac cgctcggcca gcacgccaat cgcgctgcgg atcgtcgcgc
     3481 ggctgacgtt gaac
//
LOCUS       DAOQFI010000109         3480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12724_length_3480_cov_2.686131, whole genome shotgun sequence.
ACCESSION   DAOQFI010000109 DAOQFI010000000
VERSION     DAOQFI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12724_length_3480_cov_2.686131"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLE93_02855"
     CDS             <1..765
                     /locus_tag="PLE93_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86018.1"
                     /translation="PLFIKSPGQVKPEVTDRRTCATDILPMIADQLGIEYPWPVAECD
                     PERVTVVNSPNGEGFTSPATMLRQRQKLLINRIQSVFGTGHGWGPYYRFGPHRELIGK
                     KVKNLEVLPLKRWRALPDERNAAKNYDPTAPTLRGLLQRGVTDRIPEGSVLAVAVDGK
                     IQAVGWTFKDGTGRGPGFSILLPPESLRAGFNRIAIYLLRDNGKKLRLVYDGSRPLAP
                     ELVEAQNEARAEYRREMGLPALPDPLSTGGQNGDGS"
     gene            755..1246
                     /locus_tag="PLE93_02860"
     CDS             755..1246
                     /locus_tag="PLE93_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH86019.1"
                     /translation="MGPETPLRIAGPADIGPVTRLIAGFRDFLADDSPTNAEIEATVG
                     VLLRDRSTEFLLVGDPEAGFVQTRFRLSVWTGNEDAWLEDLFVDESARGKGYGRTLVE
                     AAVERARSRGCDRIQLDVNQANEPAVKLYESCGFRPVHNPEKWGSSPDFFYTREIQRQ
                     QVG"
     gene            complement(1196..2554)
                     /locus_tag="PLE93_02865"
     CDS             complement(1196..2554)
                     /locus_tag="PLE93_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNH86020.1"
                     /translation="MSEPPKPRPSGRCGDLSPVSLQWLEQADPVSRKARGQYLTPRPV
                     AEALIDRVGLQPGLRVLDPGVGTGELLRALLDREPDLEVTGWDTDPTAIRAAGELVPE
                     ARLKLRSALDPDAGSSTPAGSSGPDGTETGGFDLVIGNPPYFQLRLDPEERKRFAGVI
                     SGRPNIFALFFQIGLQELKPGGTLAFIVPPSMNSGAYFEALREYIAARAKITDLTILE
                     GTGLFEGANTAIQLLVLRKNGPEEPVGRISGTGDGNPTHRGFIYRREVEAAGFRRVLF
                     GPDPGRLAAEFEGRSSLSELGLEATTGQVIWNENRARLFPDPSPGRVPLIWSRSIGEG
                     ELRTLSPSAGKPGFYDTLDGGRPPLLGPAIVVNRVVGAVGRGELRAALVPEGMEFLAE
                     NHVNVIRSRGPAVDWEGVLAALRMPGVVERVRLLTGNTQISARELTHLLPLDLAGVEE
                     VG"
     gene            2663..>3480
                     /locus_tag="PLE93_02870"
     CDS             2663..>3480
                     /locus_tag="PLE93_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86021.1"
                     /translation="MSRLATTAAGLFILVLGVAVTSAAEAKEIAAYPSPNVRVASDTT
                     TISFRGIKPKALGRIIVRGSRSGGHKGRLRVHSDGRGVSFIPRKKFDPKEKVTVISKR
                     NTFFGTGKRSKHRYSFVTGLLQPKGWRDEPQVPPQGERPPEWTTYKSFPLLVPKITIN
                     TSKPGVANGDIFMAPRTNGPMIVDNDGGLVYYRPGQRVTDFRTQTFRGKPVLTWWRRA
                     QVGKHVESNYAIAGTDYKVIKRFEAGNGYTSDPHEFTMASDTTAYVNSFRNVIM"
BASE COUNT          617 a         1191 c         1134 g          538 t
ORIGIN      
        1 ccgctcttca tcaagtcacc cgggcaggtc aaaccggaag tgaccgaccg gcgcacctgc
       61 gcgaccgaca tcctgccgat gatcgccgac cagctcggga tcgagtaccc gtggcccgtc
      121 gccgaatgcg acccggagcg ggtcaccgtg gtcaattcgc cgaacgggga aggtttcacc
      181 tcccccgcga cgatgctgcg gcagcggcag aaactgctga tcaaccggat ccagagcgtc
      241 ttcgggaccg gtcacggctg gggcccctac taccgcttcg ggccccacag ggaactgatc
      301 ggcaagaagg tgaagaacct cgaggtgctg ccactgaagc gctggcgcgc cctgccggat
      361 gaacggaacg cagccaagaa ctacgacccg accgccccca ccctgcgcgg cctgctccag
      421 cgcggagtca ccgaccggat cccggaaggg agtgtgctgg ccgtggcagt ggacgggaag
      481 atccaggcgg tcggctggac cttcaaggac ggcaccggcc gcgggcccgg gttctcgatc
      541 ctgcttcctc ccgagtcgct cagggccggc ttcaaccgga tcgccatcta cctgctccgg
      601 gacaacggga aaaagctccg cctggtctat gacggttcca ggccgctggc acccgagctg
      661 gttgaagcgc agaacgaggc tcgggccgag taccgcaggg agatgggact gcccgccctg
      721 ccggacccgc tttcgaccgg aggccagaac ggtgatgggt cctgagaccc cgctcagaat
      781 cgccgggccg gccgatatcg gaccggtcac ccgcctgatc gccggtttcc gggatttcct
      841 cgcggacgac tcgccgacca acgccgagat cgaagcgacg gtcggggtcc ttttgcggga
      901 ccgctcgacc gagttcttgc tggtcggcga ccccgaggcc gggttcgtgc agacccgttt
      961 ccggctttcg gtctggaccg gcaacgagga cgcctggctg gaagacctct tcgttgacga
     1021 gtcggctcgc gggaagggtt acgggcgcac cctggtcgag gcggcggtcg agcgggccag
     1081 gtcgcggggt tgcgaccgga tccagctcga cgtcaaccag gccaacgagc cggcggtgaa
     1141 gctctacgaa tcctgcggat tccggccagt ccacaatccg gagaagtggg gaagttcacc
     1201 cgacttcttc tacacccgcg agatccagcg gcagcaggtg ggttagttcc cgggccgaga
     1261 tctgggtgtt cccagtcagc agccggaccc tttcgaccac tcccggcatc cgcaacgcgg
     1321 cgaggacgcc ttcccagtca accgccgggc cacggctgcg gatcacgttg acgtggttct
     1381 ccgccaggaa ctccatcccc tccggaacga gcgccgcccg cagctccccc cggccgaccg
     1441 ccccgaccac ccggttgacc acgatcgccg ggccgagaag cggcggtcgg ccaccatcga
     1501 gcgtgtcgta gaagcccggt ttcccggcgc tcggggagag ggtccggagt tccccttcgc
     1561 cgatgctgcg ggaccagatc agcggaaccc ggcccggact ggggtccggg aacagccggg
     1621 cccggttctc gttccagatc acctggccgg tggtggcttc gaggccaagc tccgagagcg
     1681 agcttcgccc ctcgaactcg gccgccagcc ggcccgggtc gggaccgaac agaacccgcc
     1741 ggaaacccgc cgcctcaacc tcccgccggt agatgaaccc ccggtgggtc ggatttccgt
     1801 cacctgtacc ggaaatccga cccaccggtt cttcggggcc gttctttctc aggaccagga
     1861 gctggatcgc ggtgttggcg ccttcgaaga ggccggttcc ctcgaggatg gtcaggtccg
     1921 tgatcttcgc ccgggccgcg atgtattcgc gcagggcctc gaaataggcg ccgctgttca
     1981 ttgacggcgg gacgatgaag gcgagcgtgc cccccggttt cagctcctga aggccgatct
     2041 gaaagaagag cgcgaagatg ttgggccggc cggagatcac cccggcgaaa cgcttcctct
     2101 cctctgggtc gaggcgaagc tggaaatagg gcgggttccc gatcaccagg tcaaagccac
     2161 cggtttcggt cccgtccggg ccggagctcc ctgccggggt ggagctgcct gcgtctggat
     2221 cgagcgcgga gcggagcttc agacgcgcct cgggaaccag ttcgcctgcg gcgcggatcg
     2281 cggtcggatc ggtgtcccag ccggtcacct cgaggtccgg ttcccggtcc agcagggccc
     2341 ggagcagctc gccggtgccg acccccggat cgaggactct cagaccgggc tgaagcccga
     2401 cccggtcgat cagcgcctcg gcgaccggcc gcggggtcaa atactggccc cgggccttac
     2461 gggaaaccgg atcggcctgt tcgagccact gaaggctgac cggggagagg tcgccgcaac
     2521 ggcctgaggg gcgcggtttg ggcggctcac tcaaagcggc gccatcgtat ctgcctgaag
     2581 agtggccgct ccacccgcaa cttccggcgc tttgagtggt ctaagattgc ggatccgcca
     2641 atcacgatga gaaagggagc aggtgtcgcg cttagccacg accgcagcag gcctgttcat
     2701 tcttgtactt ggtgtagccg tcacttcggc tgccgaggca aaggaaatcg ccgcataccc
     2761 cagtccgaac gtccgggtcg cttcggatac gaccaccatc tcgttccgcg gcatcaagcc
     2821 gaaggcgctt ggcaggatca tcgtgcgcgg ctcccgcagc ggcggccaca agggccggct
     2881 tcgcgtccat tccgacggcc ggggcgtcag cttcatcccc cggaagaagt tcgacccgaa
     2941 ggaaaaggtg accgtgatct cgaagcggaa caccttcttc ggcaccggca agcgcagcaa
     3001 gcaccgctac agctttgtga ccggtctgct ccagcccaag ggctggcggg atgagccgca
     3061 ggttccgccg cagggcgagc ggccgcccga gtggaccacc tacaagtcct tcccgctgct
     3121 ggtcccgaaa atcacgatca acaccagcaa acccggcgtg gccaacggtg acatcttcat
     3181 ggcgccccgc accaacggtc cgatgatcgt cgacaacgac ggcggcctgg tctactaccg
     3241 gcccggccag cgggtcaccg acttccgcac ccagaccttc aggggcaagc ccgtcctgac
     3301 ctggtggcga cgcgcccagg tcggcaagca cgtcgagagc aactacgcga tcgccggtac
     3361 cgactacaag gtgatcaagc gcttcgaagc cggcaacggc tacaccagcg acccgcatga
     3421 gttcaccatg gccagtgaca ccacggccta tgtgaactcc ttccgcaacg tgatcatgga
//
LOCUS       DAOQFI010000110         3467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12781_length_3467_cov_2.947245, whole genome shotgun sequence.
ACCESSION   DAOQFI010000110 DAOQFI010000000
VERSION     DAOQFI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12781_length_3467_cov_2.947245"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLE93_02875"
     CDS             complement(<1..880)
                     /locus_tag="PLE93_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86022.1"
                     /translation="MSLGRGFYRLLGSLVCAIAFFAGVGNASAELIDSEDLGDGVQRL
                     TYRIGPIEVTSGQNRIVYKMVTAEERPPVDGYITAFRPNLVNPDGTIPKSSSIMFHHG
                     VWINLSRRDATSGGMSERFIGTGEEKTEVVFPPGFGYFHKGTDVWLLNHMIHNLTPAE
                     HTLYITYQIDFVPLTSPKAEGMRGVRPIWMDVMNPNPYPVFDTYKGSGGKDGKIVWPY
                     EAKEDPYKDGIKRNLWTVDRDGVLVWSVGHVHTGGLATDLYLNRDGAKYAGPKCPKPR
                     ILRKKLKKNAPRSLRSA"
     gene            complement(906..2033)
                     /locus_tag="PLE93_02880"
     CDS             complement(906..2033)
                     /locus_tag="PLE93_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH86023.1"
                     /translation="MSRRVLILGAGFGGLELGTTLSEELGDQVEVTLIDRNESFSFGY
                     SKLDIMFRGASRDSVSLPYSAVAKTGVTLLREEITAIDPETRTVTTDAGTHQGDALVI
                     ALGADYDLDSTPGLAERGHEFYSFAGAEAAAGPIADFDSGKAVIAVCGAPFKCPPAPS
                     ECALMLDDELTRRGVREQCEITLTMPLGTPVPPSPETSAALKAEFGQRGITFLGDMRP
                     KRVEPDRIIYQDGTELPFDLLLGVPKHRAPEVVIDSGITEDEYVPVDSATLKTRFEGV
                     YAVGDVATAGVPKAGVFAEGAAKVVARELVAEIRGEASPEPYDGRGSCYIEFGEDRIA
                     RVDIDFLSGPEKTGVFNAPSLDLRKEKEEFGSSRKSRWFNR"
     gene            2198..>3467
                     /locus_tag="PLE93_02885"
     CDS             2198..>3467
                     /locus_tag="PLE93_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH86024.1"
                     /translation="MAAENPGSIRPLARGTLKLQSDERLLKLFRRGVEPAFEELINRY
                     RVTLVAYAGSIAGPDRGEDVVQDALVKAHRSLRDDRDIDPRPWLYAVVRNTALNDVRD
                     NRKHRHDGLGETATGGTSPDELVEQRERFAAVLAAVADLPPAQRRALVDHELSGFSHE
                     EIAAELDLSTGATKQLIYRARLSLRNAFGAMIPIPLIAWLAEGSGVVATGAAGTGATA
                     TGLMGAIGGGSAAKLAVVAVVAGGSLAGGIAVERHQADRGTHPDTAQAAEPGDSGAAA
                     PNGEDSGGDDGPGNGSLVADRSRTGSDKGSDGAEGRGESDRGSQGGRQTGEGGRDSGD
                     SRPGGSGSSGPGSGGSGGDDHPLGVRPSGGRHEPGEDSDEDSGNGGHGDDRPSRGVSG
                     GGPGPSGGPPREDDSSGHGPSGGSDESDDSD"
BASE COUNT          559 a         1125 c         1190 g          593 t
ORIGIN      
        1 gcgcgctccg cagcgagcgg ggggcgttct tcttcagctt cttcctgagg atccgcggct
       61 tcgggcattt cggccccgcg tacttcgcac cgtcgcggtt caggtaaagg tccgtcgcga
      121 ggccgccggt gtgaacgtgg ccgaccgacc agaccagcac gccgtcccgg tccacggtcc
      181 agaggttgcg cttgatcccg tccttgtagg gatcctcctt ggcttcgtaa ggccagacga
      241 tcttgccgtc cttgccaccc gatcccttgt aagtgtcgaa gaccggatag ggattcgggt
      301 tcatcacgtc catccagatc gggcgcaccc cgcgcatccc ctcggccttt ggcgaggtca
      361 gcgggacgaa atcgatctgg taggtgatgt agagagtgtg ttcggccggc gtcaggttgt
      421 ggatcatgtg gttgaggagc cagacgtcgg tccccttgtg gaagtaaccg aagccgggcg
      481 ggaagacgac ctcggtcttc tcctcgccgg tgccgatgaa ccgctcggac attccgcccg
      541 aagtggcgtc gcgacgggag aggttgatcc agaccccgtg gtggaacatg atgctgctcg
      601 atttcgggat ggtcccgtcc gggttgacaa ggttcggccg gaaggccgtg atgtacccgt
      661 caaccggcgg ccgctcctcg gcggtgacca tcttgtagac gatccggttc tggccggaag
      721 tgacctcgat cggaccgatc cggtaggtca ggcgctggac gccgtcaccc agatcttccg
      781 aatcgatcag ctccgccgag gcgttgccga ccccggcgaa gaacgcaatt gcgcagacga
      841 ggctgcccag cagtctgtaa aaccctctcc ccaaggacat ggggaaagag tagcgccagt
      901 gtcagttatc ggttgaacca gcgtgatttg cggctcgaac cgaactcttc cttctccttg
      961 cgaaggtcga gtgacggcgc gttgaagacc ccggtctttt ccggcccgga gaggaaatcg
     1021 atgtcaacgc gggcgatccg gtcctcgccg aattcgatgt agcaggagcc cctgccgtcg
     1081 tagggttccg ggctggcctc gccccggatc tccgccacga gttcgcgggc gactaccttc
     1141 gccgccccct cggcgaacac tcccgccttg ggcacgccgg ccgtggcgac gtccccgacc
     1201 gcatagaccc cttcgaagcg ggtcttcagg gtggcggagt cgaccggcac gtattcgtct
     1261 tcggtgatgc cggaatcgat caccacttcc ggggcccggt gcttcggcac gccgagcagc
     1321 aggtcgaagg gaagctcggt cccgtcctgg tagatgatcc tgtccggttc gacccgcttg
     1381 ggccgcatgt caccgaggaa ggtgatgccc cgctgcccga actccgcctt gagggcggcg
     1441 gaggtttccg gcgagggtgg caccggtgtg ccgagcggca tggtcagggt gatctcgcat
     1501 tgctcgcgaa ccccgcgccg ggtcagttcg tcgtccagca tcagggcgca ttcgctcggg
     1561 gccggcgggc acttgaaagg agcgccacag accgcgatca ccgccttgcc ggaatcgaag
     1621 tcggcgatcg ggccggccgc ggcttcggcc ccggcgaagg aatagaactc gtgtccccgt
     1681 tcggccaggc ccggggttga atcgaggtcg taatcggcgc ccagcgcgat gaccagggca
     1741 tctccctgat gggttccggc gtcggttgtg accgtgcggg tttccgggtc gatcgccgtg
     1801 atctcttccc tgaggagggt gaccccggtt ttggccaccg ccgaataggg caggctgacc
     1861 gagtcccggc tggccccgcg gaacatgatg tcgagcttcg agtagccgaa agagaacgac
     1921 tcgtttcgat cgatcagggt gacctcgacc tgatccccca gctcttcgga gagcgtcgtg
     1981 cccagttcga gtccgccaaa gcccgctccc agaatcagta cccgtctgct cacggggcca
     2041 gcatactgcc gcccgcaacc ccaggcaggc agcggtgtgg cctgcgatta cccaaccgtg
     2101 ccgggacggc ggatgcgtga cttccgtgtc cggttcgggt tgcaggttca gattctcacg
     2161 tggctcttat ggtcacggcg taacctgagg ggaagagatg gcggcagaaa atccaggatc
     2221 gatccggcca ctggcccggg gcacgctgaa actccagtcg gatgagcggc tgctgaagct
     2281 gtttcgtcgc ggagtcgaac cggccttcga ggaactgatc aaccggtacc gggtcaccct
     2341 ggttgcctat gccggatcaa tcgccgggcc ggaccggggc gaggacgtgg tccaggacgc
     2401 tctggtcaag gcgcaccgtt cccttcggga tgaccgtgac atcgacccac ggccctggct
     2461 ttacgcggtc gtccggaaca ccgctctgaa tgacgtgcgc gacaaccgca agcaccgtca
     2521 cgacgggctc ggcgagacgg ccaccggtgg aacctccccg gatgagctgg tcgaacagcg
     2581 cgaacggttc gccgccgtac tcgccgcggt ggccgacctc cctcccgcac agcgccgggc
     2641 cttggtcgat cacgaactga gtggtttctc acacgaggag atcgcggccg aactcgatct
     2701 cagcacgggg gcaaccaagc aactcatcta ccgggcccgg ctttcgcttc gcaacgcctt
     2761 cggggcgatg atcccgatcc cgttgatcgc ctggctggcc gaaggctcgg gggtggtggc
     2821 caccggcgcc gcgggcaccg gagcgacggc gaccgggctg atgggggcga tcggtggcgg
     2881 cagcgccgcc aagctggccg ttgttgccgt ggtcgcgggc gggtcgctgg ccggcggcat
     2941 cgcggttgaa cgccatcagg cagaccgcgg tacccatccg gacaccgccc aggcggccga
     3001 gccgggcgat tccggcgccg cggctccgaa cggcgaggat tccggcggcg atgacggtcc
     3061 cggtaacggc agcctggtcg cagaccgaag ccggacgggt tccgacaagg gatcggatgg
     3121 cgccgaaggc cggggcgaga gcgatcgcgg cagtcaaggg ggccgtcaga caggtgaagg
     3181 cggccgcgac tcaggcgact cccgccccgg cggttccggt tcctcgggcc cgggttcggg
     3241 cggttcaggt ggcgatgacc atcccctggg cgtccgaccc tccggcggcc ggcatgagcc
     3301 cggggaagat tcagacgagg attccgggaa cggcggccac ggcgacgatc ggccctcccg
     3361 cggggtcagt ggcggtggtc ccggcccgag cggcggtcct ccgcgcgagg atgactccag
     3421 cggccacggc ccctccggcg ggtcggacga atccgacgat tcggact
//
LOCUS       DAOQFI010000111         3442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12959_length_3442_cov_3.539415, whole genome shotgun sequence.
ACCESSION   DAOQFI010000111 DAOQFI010000000
VERSION     DAOQFI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12959_length_3442_cov_3.539415"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLE93_02890"
     CDS             complement(<1..650)
                     /locus_tag="PLE93_02890"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNH86025.1"
                     /translation="MANPEITVCGRLTVAWDGEQLEGELPGRQGRLIFAYLVLNRSRP
                     VRRDELVEALWADEGLPSGGEALLAPPLSRLRKALGKDRLQGRADLSLQLGEEARIDF
                     EVAQQKLASAQNVAGNPDATADQLEAGWDDSREAAGILEGGLLPGLEARWIEEHRLYF
                     EELRLKSLETVARIGARLGTAQQAQAERAARNAVEASPFRESARAALIELLEAQGN"
     gene            complement(692..>3442)
                     /locus_tag="PLE93_02895"
     CDS             complement(692..>3442)
                     /locus_tag="PLE93_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH86026.1"
                     /translation="ASPEAAAAVPAERQATPEQIGRLIDPRIGEIGLVGREEILAQLN
                     HELDLAVAGDLRIALLAGDGGMGKTRIAAELAAGRDDVTVLYGRSEPDEIRPFRIWSG
                     LLRSAMRQAGDIPPAEIVGGDGPTLARILPELVRSMELPAPGPAGDLESERRALFGAV
                     MRMIGRLTARQPMLIVLDDLHWADRSTLMLLASLAGDNPPGGVLALGIYRDTELPEDS
                     LLPETLTNLQRRLPTLKLEVEALGSEDVGQLIGGRLDAALAGSLHDQTGGNPFLIEQL
                     VRHLEETGIGDPGNAPAEVRQIISQRVNHLPDGGPDLLRRAALIGRDFDLSILLETTS
                     WDEDSVIDLLDAAVAAGLLDESPTVPGRYSFVHALLRSTLEEELGLTRRAMIHRDIGE
                     AIERQTASRPEKRVGEKAWHFTQAGPAEADRAVHWATQAAEQAEARLAYDEAVDFFDG
                     AIAAARADEPVDQGRLARLLLSQAKAKWKNGALQAAGDTFLEAAKAARESGLPELAAQ
                     AAIGSRWGGWDAFDADLAEQLSLMRQALGSLPPVDSPLRAELMAMLGHTLYYGSGQAD
                     EARQLAAEAIEMISRIDAPEAEFGVIQGTAFLRWQPLNRDQRLPASERMLEIAERLDD
                     HELIGQALVSRQLSLLNLGRGAEAEADRSRHYAMSETLPQIRVISKAMRACSWFMTGD
                     WQDGEDLFSELEAEGMPRGAALLLEDALRFMVRAQQGALAEHLGRLELFEAGAKGWEM
                     WPTWRLGLLLAKAQAGQLDEVRELIAEKGYESLDETRDINATLLPFCAVGNMLAGELE
                     DRDGAAKLVDLLTPFSGEWIMIARIGSTLGPVDMHLGELHLLCGEHREAATALERSLV
                     ACEAMDAVPYLARTKLALASAFGEMGDPDGQERRMELRNEGEQVAKRLDMQAILRRHI
                     RS"
BASE COUNT          476 a         1201 c         1182 g          583 t
ORIGIN      
        1 atattcccct gggcttcgag cagttcgatc agcgcggcgc gggccgattc gcgaaacggg
       61 gaggcctcta ccgcgttccg ggccgcccgt tctgcctggg cctgctgggc ggttccgagg
      121 cgggccccga tccgggccac ggtttcgagc gacttgaggc gaagctcctc gaagtagagc
      181 cggtgctcct cgatccagcg ggcttccagg ccgggcagca ggccgccctc gagaatgccg
      241 gcggcttcac gggagtcgtc ccacccggct tcgagctgat ccgctgtcgc atccgggttc
      301 ccggccacgt tctgggccga ggcgagcttc tgttgggcaa cctcgaagtc gatccgtgct
      361 tcctccccga gctgaagcga aaggtcggcc cggccctgca gccggtcctt gcccagggcc
      421 ttccgcagcc gggagagcgg aggcgccagc aacgcctcgc cgccgctggg caggccctcg
      481 tcggcccata gcgcttcgac cagttcatcc ctgcgcacgg gtcgggaacg gttcagcacc
      541 aggtaggcga aaatcagtcg cccctgacgg ccgggaagct ctccttccag ttgctcacca
      601 tcccatgcca cggtgagccg gccgcaaacc gttatttccg gatttgccac tgcggcaact
      661 tatccaaccg gcccggtcgg cgcgaatcgg ttcaggaccg gatgtgacgc ctgaggatcg
      721 cctgcatgtc gagtctcttc gcgacctgct cgccctcgtt tcgaagctcc atccttcgtt
      781 cctgcccgtc cggatcgccc atttccccga aggctgaggc gagggccagt ttggttcgcg
      841 cgaggtaggg gacggcatcc atcgcctcgc aggccaccag cgaccgttcc agcgcggtag
      901 ctgcctcgcg atgctctccg caaaggaggt ggagctcccc gaggtgcatg tcgaccggcc
      961 cgagagtcga cccgatccgg gcgatcatga tccattcgcc gctgaagggg gtgagcagat
     1021 cgaccagctt cgccgccccg tcgcggtcct ccagctcgcc ggccagcatg ttgccgaccg
     1081 cgcagaaggg gagcagggtc gcgttgatgt ccctcgtctc atccaggctt tcatagccct
     1141 tctcggcgat cagttcgcga acttcatcga gctggccggc ctgggccttg gcgagcagca
     1201 gtccgagccg ccaggtcggc cacatctccc agcctttcgc gcccgcctcg aagagctcga
     1261 gccggccgag gtgctcggcc agggcgccct gctgcgcccg gaccatgaag cggagcgcgt
     1321 cttcgaggag cagggcggct ccgcggggca tcccttccgc ctcgagttcg ctgaagaggt
     1381 cctcgccgtc ctgccagtcg ccggtcatga accaggagca ggcccgcatt gcctttgaaa
     1441 tcacccggat ctgcggcagt gtttcgctca tcgcgtagtg gcgactgcga tcggcttcgg
     1501 cttcggcacc gcggccgagg ttgagcaggg aaagctgccg ggagaccagg gcctggccga
     1561 tcaattcatg atcgtcgaga cgctcggcaa tttccagcat ccgctccgag gccgggagtc
     1621 tctgatcccg gttgagtggc tgccagcgca gaaaggcggt tccctgaatc acgccgaatt
     1681 cggcttccgg agcgtcgatg cgggagatca tctcgattgc ctcagcggcc agctgccggg
     1741 cttcgtcggc ctggccgctg ccgtagtaga gggtgtgacc cagcatcgcc atcaattcgg
     1801 cccggagcgg gctgtccacc gggggcaggc ttcccagggc ctgtctcatc agcgagagct
     1861 gctcggccag atcggcgtcg aaggcatccc agccgcccca gcggctgccg atcgccgcct
     1921 gggcagccag ctcgggcaga cctgattccc gggccgcctt cgccgcctcg aggaaggtgt
     1981 cgccggctgc ctgcagggct ccgttcttcc atttcgcctt ggcctggctg agcagcagcc
     2041 gggccagccg tccctggtcg accggctcgt cggcccgggc cgcggcgatc gccccgtcga
     2101 agaagtcgac cgcctcgtcg taggcgagcc gcgcttccgc ctgttcagcc gcctgggtcg
     2161 cccagtggac ggcccggtcg gcttcggcag gccccgcctg ggtgaaatgc caggccttct
     2221 ccccgacccg cttctccggg cggctcgcgg tctggcgttc gatcgcttcg ccgatgtcgc
     2281 ggtggatcat cgcccggcgg gtcaacccca gttcctcctc cagggtgctg cggagcaggg
     2341 cgtgtacgaa cgagtagcgg ccggggacgg tcggggattc gtcgagcagc ccggcggcga
     2401 cggcggcgtc gagcaggtcg atcaccgagt cctcgtccca gctggtcgtc tccaggagga
     2461 tcgaaaggtc gaagtcgcgg ccgatcaggg cggcccggcg gagcaggtcg gggccgccat
     2521 cgggcaggtg gttgacccgc tgggagatga tctgccggac ctcggccggg gcgtttccgg
     2581 ggtcgccgat gccggtctcc tcgaggtgcc ggaccagctg ttcgatcagg aaggggttgc
     2641 cgccggtctg gtcgtgaagc gaaccggcca gggccgcgtc cagtcggccg ccgatcagct
     2701 ggccgacgtc ttcgcttccg agcgcttcaa cttccagctt cagggtcggc agccggcgct
     2761 ggaggttggt cagggtctcc ggcagcaggc tgtcctcggg cagttccgtg tcccggtaga
     2821 tgcccagggc gagcaccccg ccgggcgggt tgtcgccggc caggctggcc agcagcatca
     2881 gggtcgagcg atccgcccag tgaaggtcgt cgagcacgat cagcatcggt tgccgggcgg
     2941 tcagccggcc gatcattcgc atcaccgcgc cgaacagggc ccggcgctcg gactcgaggt
     3001 caccggccgg tcccggggcc ggcagttcca tgctgcggac cagctccggc aggatccggg
     3061 ccagggtcgg gccgtcgccg ccgacgattt cggccggggg gatgtccccg gcctgccgca
     3121 tcgccgagcg gagcaggccc gaccagattc ggaaggggcg gatctcatcc ggttccgagc
     3181 ggccgtagag gacggtcacg tcatccctcc cggcggccag ctcggcggcg attctggtct
     3241 tgcccatgcc gccgtcgccg gccagcaggg cgatccgcag gtcacccgcg accgccaggt
     3301 ccagttcatg gttcagctgg gccaggatct cctcgcggcc gaccagcccg atttcgccga
     3361 tccgggggtc gatcagccgg ccgatctgct ccggggttgc ctgccgttca gccgggaccg
     3421 cggccgcagc ctccgggctg gc
//
LOCUS       DAOQFI010000112         3435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_12996_length_3435_cov_3.348521, whole genome shotgun sequence.
ACCESSION   DAOQFI010000112 DAOQFI010000000
VERSION     DAOQFI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12996_length_3435_cov_3.348521"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLE93_02900"
     CDS             <1..190
                     /locus_tag="PLE93_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86027.1"
                     /translation="SVIADFHVQLSSFLIAGAIAATHPATSVQVAGASGLAGTEAITG
                     KQERDLLKRAAAFFAADQ"
     gene            187..1089
                     /locus_tag="PLE93_02905"
     CDS             187..1089
                     /locus_tag="PLE93_02905"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNH86028.1"
                     /translation="MREKYRLVEAEKARTPINVSCQLLGVSRSGYYSWATRVPSRREL
                     EDRDLTRRIREIHQANRGVYGSPRIHAELRMDHGIRVSRKRVERLMRKAEISGLVPRK
                     RGKTTIRVPGVRVAGDLLLRDFTATGPNQVWVADITYLKTWEGWIYLAAIQDLFSRRI
                     VGWSMADHMRAELVVDALEMAVHRRKPDPGLIHHSDQGSQYVSLAFGQMTRKNGINRS
                     MGHVGSAYDNAVSETFFATLKKELVHRRPWPRKQELRSEVFEFIEGFYNRKRKHSYLG
                     WLSPADFEDRASPVLDQPEPISSN"
     gene            1337..1645
                     /locus_tag="PLE93_02910"
     CDS             1337..1645
                     /locus_tag="PLE93_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HNH86029.1"
                     /translation="MKLSTNSKTVRRGKKATFKISVHNVGNAWARNVRVCASAPKNLV
                     QIPKCVSLGSVAADNFKTASLQITVKKNANKGKRATVTFTASGQGMTSKSARASVKVQ
                     "
     gene            1707..2027
                     /locus_tag="PLE93_02915"
     CDS             1707..2027
                     /locus_tag="PLE93_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86030.1"
                     /translation="MSEEATEQKIPKGSYAAGRRVTIPEGAEQPIVVFINGVAQSEDQ
                     DYVLRGNEILFTREIIKEEKSGKKKLVMLLGVVGFYNKDETIDVQYQLNGKTELAGDL
                     SVSK"
     gene            2173..2682
                     /locus_tag="PLE93_02920"
     CDS             2173..2682
                     /locus_tag="PLE93_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86031.1"
                     /translation="MNFQSRIVIAFIVAIGAAVALSACSGSQPERVSGSSGGDAPTVK
                     VEFNGSDSTPSITSSDYDYSGYDDSTYDSGYDSSYGDSGYDDASYDSSYDDSYSGSSC
                     GSNLSYKLSTTTPGFAQNVADAHCDEIGSGSGYVTAYSPTMNQDYEMYCSGSPHVCTG
                     GVDAEVYFD"
     gene            complement(2745..3227)
                     /locus_tag="PLE93_02925"
     CDS             complement(2745..3227)
                     /locus_tag="PLE93_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86032.1"
                     /translation="MLVLLTAAVLPATETFAYPSGELVPGPVKQVASSTLRARHLTAF
                     NSPDRKTFCLSSPSYSLDCGTIPKFPRKSPEHSGWIDARTGKVTLCFIAGANLANSCF
                     VNWNNNSPVLHFGQKNRIGPFRCVSRHRGITCIKVTGRGAGKGFRINRKRAVKVRISV
                     "
     gene            complement(3253..>3435)
                     /locus_tag="PLE93_02930"
     CDS             complement(3253..>3435)
                     /locus_tag="PLE93_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86033.1"
                     /translation="IDTAIHTRQHADKVIRPEVEYCGILEWSQLDSVREAGRKAARGA
                     LESMPETFGKGSNVKP"
BASE COUNT          762 a         1045 c          994 g          634 t
ORIGIN      
        1 gtccgtgata gcggatttcc atgttcagct ctcctcgttc ttgattgccg gcgcaatcgc
       61 agcgacccat cccgccacct cggtccaggt tgccggtgcg agcggcctcg ccggaaccga
      121 ggcaattacc gggaaacagg aaagggatct cctcaaacga gccgcggcct tcttcgcggc
      181 cgaccagtga gagagaagta tcggctggtc gaagcggaga aggccagaac cccgatcaac
      241 gtgagctgcc aactgctggg agtttcccgg tccgggtatt actcgtgggc aacgagggtg
      301 ccgtcaagac gggagcttga ggatcgggat ctgacccgga ggatccgaga gatccatcag
      361 gctaaccgtg gtgtttacgg gtcaccgaga attcacgcgg agcttcggat ggatcacggc
      421 atccgggttt cacgtaaacg ggttgaacgg ctgatgcgaa aggccgagat cagtggtctg
      481 gtgccgcgca agcgcgggaa gacgacgatc agggtgcccg gggtcagggt tgccggtgac
      541 ttgctcttgc gtgatttcac tgcgaccggc ccgaaccagg tttgggtggc ggatatcacc
      601 tacctgaaaa cctgggaagg ctggatctac ctggcagcga ttcaagacct gttctctcgc
      661 cggatcgttg gctggtcaat ggccgatcac atgagagcag aactcgtcgt tgacgctctc
      721 gaaatggccg ttcaccgaag gaaacccgac ccgggtctga ttcatcactc cgatcagggc
      781 agccaatacg tttccctcgc gttcggtcaa atgacccgaa agaacggcat caaccgctca
      841 atggggcacg tcggatcggc ctacgacaac gcggtctcgg agacattctt cgcgaccctc
      901 aaaaaggagc tggttcaccg acgtccctgg ccgagaaagc aggagctccg ctctgaggta
      961 ttcgagttca tcgaaggctt ctacaaccgc aaacgaaaac acagctacct cggctggctc
     1021 tcaccagccg acttcgagga ccgggctagc ccggtcctcg accaacccga accgataagt
     1081 tcaaactgac acgatcaaac tgtccgaagg aacgggggag ttccagtgga ggcgttgcga
     1141 cagttcggga gcaaactgtt cggcgatccc gggagcggat caacagacct acgtgctcac
     1201 ccagcaggat gttggatcga caatcagagt cactgaaact gcatccaaca gctcagggtc
     1261 ttcgactgcg gccgtgtccc agaagaccgc cgtagtgacc agccccaact ctggcaaaaa
     1321 gccaatcttc ggcccgttga agctctcaac gaattcgaag acggtccgtc gcggcaagaa
     1381 ggcaaccttc aagatctccg ttcataacgt cggcaatgcc tgggccagaa acgtcagagt
     1441 ctgcgcttcg gcccctaaga acctggtcca gattcccaag tgcgtgagtc tcggttctgt
     1501 ggcggccgac aacttcaaga ccgccagtct tcagatcacg gtcaaaaaga acgcgaacaa
     1561 gggcaagcgc gccaccgtta ccttcaccgc gagcggtcag ggcatgacca gcaagtccgc
     1621 cagggccagc gtcaaggtgc agtagacgcc cgggccagcg gcgacgatcc ccttcgcagc
     1681 cgaatcggcg agaatgtgac tcgccgatga gtgaagaagc aaccgaacag aagatcccca
     1741 aaggctccta cgccgccgga aggcgggtga ctatccccga aggcgccgaa cagccgatcg
     1801 tcgtcttcat caatggcgtc gcccagagcg aagaccagga ctatgtgctg cgcggcaacg
     1861 agatcctttt cacccgggag atcatcaagg aagagaaatc cggcaagaag aaactggtca
     1921 tgctgctagg agtggtcggc ttttacaaca aggacgaaac gatcgacgtc cagtatcagc
     1981 tgaacggcaa gaccgaactc gctggcgacc tttcggtttc gaagtagccg cagccagacc
     2041 ctggctggcc acctttgtgt gagtcccgtc accccaggaa gatcccctga ccaggtgccg
     2101 tctggaatag cagagtgcga tttccagatg tgtcgcactc ggacaatttc cgacacatga
     2161 gggggaagca ccatgaattt ccagtcacgc atcgtcatcg cttttatagt cgcgatcggc
     2221 gccgcagtcg cccttagcgc ctgtagcggc agccagcccg agcgggtcag cggcagctcc
     2281 ggcggggatg ctccgacggt gaaggtcgag ttcaacggct cggactccac cccgtccatc
     2341 accagctcgg actacgacta ttcgggttac gacgactcaa cctatgactc ggggtacgac
     2401 tcgagttatg gcgactccgg gtacgacgac gcgtcctacg actcgtccta tgacgacagc
     2461 tattccggaa gctcctgcgg aagcaacctg tcctacaagc tgtccaccac cacacccggt
     2521 ttcgcgcaga acgtcgccga cgcccactgc gatgagattg gctccggcag cggttacgtg
     2581 accgcctaca gtccgacgat gaaccaggac tacgagatgt actgcagcgg ttcaccccac
     2641 gtgtgcaccg gcggagtcga cgccgaggtc tacttcgact gaccccatcc ggaatccacg
     2701 agaaagaaaa ggggcgcccg ctccgggcgc ccctttttcg ttgctcagac cgaaatccgg
     2761 accttgaccg cccgcttgcg gttgatccgg aaccccttgc cggcgccacg accggtcacc
     2821 ttgatgcagg ttatgccgcg gtgccgggaa acgcatctga agggaccaat ccggttcttc
     2881 tggccgaaat gaagaaccgg agagttgttg ttccagttca cgaagcagga gttcgcgaga
     2941 ttcgccccgg cgatgaagca aagagtgacc tttccggtcc tggcatcgat ccaccctgag
     3001 tgttccggac ttttcctcgg gaacttcggg atcgtcccgc aatcgagcga gtaactcgga
     3061 ctcgaaaggc aaaacgtctt tcgatccggt gagttgaacg ccgtcaggtg ccttgcccgc
     3121 aaggtcgatg aagccacctg cttcaccggg ccgggcacga gttcccctga cgggtacgcg
     3181 aaggtctctg tggccggcag caccgctgct gtcaacagga ccagcatcgc gaccgcccct
     3241 gagataactg atctacggct tgacattgct cccctttccg aaagtttccg gcatcgactc
     3301 aagggctccg cgggccgcct tgcgacccgc ctcccgaacc gaatcgagtt gactccattc
     3361 cagaattcca cagtactcga cttccggacg gataaccttg tccgcatgtt gtctggtatg
     3421 gatggcggtg tcgat
//
LOCUS       DAOQFI010000113         3412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13151_length_3412_cov_4.275842, whole genome shotgun sequence.
ACCESSION   DAOQFI010000113 DAOQFI010000000
VERSION     DAOQFI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13151_length_3412_cov_4.275842"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLE93_02935"
     CDS             complement(<1..866)
                     /locus_tag="PLE93_02935"
                     /inference="COORDINATES: protein motif:HMM:NF020846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HNH86034.1"
                     /translation="MSAFSRFLTLFMLIVLSCGALVTSNAGAANKNAYAMAMLKLHHP
                     AGLQKFARSVSSPNSDNYGQYLPVSRIVKKFGARSKAARRALRYFRSRGMRATLDGAH
                     QNVLVSMDLNQAGSYLAPSASASVARQGGPGSVPPALRGVASSLDFLDPDPEKYSTAG
                     KFPPQPSGSPFPEGSSARPHSGTQAGCAAGQRGATAIGEPNRNFTPNQFLDAYGISDL
                     NQQGFKGQGRRIAVIEIDGFKRSDIEAYGDCYGKRVPPTRVYPVYYKKPLAPGAETTL
                     DLEVITAVAPQLA"
     gene            complement(903..>3412)
                     /locus_tag="PLE93_02940"
     CDS             complement(903..>3412)
                     /locus_tag="PLE93_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH86035.1"
                     /translation="SLMTAVAVIFGIALVAGTYILTDTTNQAFDQIFNESLAGTAQVV
                     TAKSEVDQQDGSTPAFPAKILKKVDQVDGVKVAAGSIFTQGAILDDQGDSIGAGFSPQ
                     FIASTGPKVFEAVTYPEGHPPTNAHEATLDQAAADRKGIELGDTIELVGTDKAVPFRV
                     VGLTQLGGASFGGSSIAQVQLPVAQRVTGKVGEYDQISIAADSGISSEELRDRVSEVV
                     PKNLRVETAQENADRNASEIRDSLQFLTIALLAFAAIALFVGSFVIFNVFSITVAQRT
                     REFGMLRTLGASRGQILRTVVIEGLIIGLVGSLLGLVVGYGLAAGLSAVLKAVGAELP
                     ANSLQVLPRTIIVSLLIGVGVTLVSSLIPAFRSTQVPPIAALSENLVVGGRNRVVVRT
                     VIAVLLGVLGLALIAMTLISGKDGGSGAAQIGAGAVCVLIAISIFAPKLVSPAARIIG
                     APLERFGGLTGRLARENAQRNPSRTAVTAAALMIGLALIAFVTIFASGLSSSVNKVID
                     DQLPGEITLQGQGGILPFPAGAIKVVQKVDGVEAASGVRYVSAKVDGKTAAISSVDPA
                     DIVKVYTVEWEEGSDSDLTGLTDGQIVIGNKLADSLDVGVGDRVTLTSQKGRKFPFQI
                     NAIMKTDSIALAGEGIIDQGAMERDFDQKSDAVGLVKLDPGADLDKTQTAIEDALTAQ
                     DFPTVDVLNQGELKEQQKSQIKGLLAMIYVLLALAVIVSLVGIVVTLILSIYERTREL
                     GMLRAVGMSRRQVKRMVRYEAVITAVIGAVSGLIVGVIFAFLIGIPLGGDGFELSYPV
                     GTLIIILILTALAGVIAAIYPARKAAKLDVLEAVSYE"
BASE COUNT          616 a         1082 c         1128 g          586 t
ORIGIN      
        1 gcaagctgcg gagcgaccgc cgtgatcact tcgagatcga gggtggtttc ggcgccgggc
       61 gccagcggct tcttgtagta gaccgggtag accctggtcg gcgggacccg cttgccgtag
      121 cagtcgccgt aggcctcgat gtcggaccgc ttgaagccgt cgatctcgat caccgcaatc
      181 cgccggccct gacccttgaa tccctgctgg ttgaggtcac tgatgccgta ggcgtcgagg
      241 aactggttgg gggtgaagtt gcggttcggt tcaccgatcg cggtggctcc gcgctgaccg
      301 gccgcgcagc ccgcctgggt tccggaatgg ggtcgggccg agctgccctc ggggaaggga
      361 ctgcccgacg gctgaggcgg gaatttgccc gcagtcgagt acttctccgg atccgggtcg
      421 aggaagtcaa ggctgcttgc cacgccccgc agggcgggag gcaccgaccc cgggccaccc
      481 tgacgggcaa ccgaggccga ggcggacgga gcgaggtagc ttccggcctg gttcaggtcc
      541 atgctcacca gtacgttctg atgcgcaccg tcgagcgtgg cccgcatgcc ccgggaacgg
      601 aagtaacgca aggcccgcct ggcggccttc gatcgggcgc cgaacttctt gacgatcctg
      661 ctcaccggta ggtactgccc gtagttgtcg ctattcggac tgctgaccga cctcgcgaac
      721 ttctgcaacc cggcgggatg gtgaagcttg agcatcgcca tcgcgtaggc gttcttgttg
      781 gcggcaccgg cattcgatgt gaccagcgcc ccacatgaaa gaacgatcag catgaacaaa
      841 gtgaggaagc gcgaaaaagc cgacatggcc ggcaagttcc cgcctggacc gcaggtcaaa
      901 cgctattcgt acgaaacggc ttccagcacg tcgagcttgg ccgccttgcg ggcggggtag
      961 atcgcggcga tcactccggc cagcgccgtg agaatcagga tgatgatcag ggtgccgacc
     1021 gggtagctca gttcgaagcc gtcaccgccg agcgggatgc cgatcaggaa ggcgaagatg
     1081 acgccgacga tcaggccgga gacggccccg atcacagccg tgatcaccgc ttcgtagcga
     1141 accatccgct tgacctgacg gcgtgacatc ccgaccgccc tgagcatgcc gagttcgcgg
     1201 gttcgctcgt agatcgagag gatcagcgtg accacgatgc cgaccagcga aacgatcacg
     1261 gccagcgcga gcagcacgta gatcatcgcc agaagtccct tgatctggga cttctgctgt
     1321 tccttcagtt cgccctggtt gagcacgtcg acggtcggga agtcctgagc cgtcaacgcg
     1381 tcctcgatcg cggtctgggt cttgtcgaga tcggcaccgg ggtcgagttt gaccagaccg
     1441 accgcatcgg acttctggtc gaagtcccgc tccatcgcgc cctggtcgat gatgccctcg
     1501 ccggccagcg cgatgctgtc ggtcttcatg atcgcgttga tctggaaggg gaatttccgg
     1561 cccttctgtg aggtcagggt gactctgtct cccaccccga catccagcga gtcggcgagc
     1621 ttgttgccga tcacgatctg gccgtcggtc agaccggtca gatccgaatc agaaccttcc
     1681 tcccactcga ccgtgtagac cttgacgatg tcggccggat cgaccgagga gatcgcggcc
     1741 gtcttgccat cgaccttcgc cgatacgtaa cggacgccgg aagcggcttc caccccgtcg
     1801 accttctgga caaccttgat cgcgccggcg ggaaacggca ggatgccgcc ctgaccctgc
     1861 agggtgatct cgcccggaag ctggtcgtcg atcaccttgt tcacggacga actgaggccg
     1921 gaagcaaaga tcgtgacgaa ggcgatcagg gcgagcccga tcatcagcgc cgcggcggtg
     1981 accgcggtgc gtgacgggtt tcgctgggcg ttctcgcggg cgaggcggcc ggtgaggccg
     2041 ccgaaccgct ccagcggcgc accgatgatc cgggccgcgg gcgaaaccag cttgggggcg
     2101 aagatcgaga tcgcgatcag cacgcagacc gctccggctc cgatctgggc ggcgccgctg
     2161 ccgccgtcct tgccactgat cagggtcatt gcgatcaggg ccaggccgag caccccgagc
     2221 agaacagcga tcaccgttct gacgacgacc cggttcctgc ctccaaccac caggttttcc
     2281 gagagcgccg cgatcggtgg cacctgggtc gagcggaagg ccgggatcag cgatgagacc
     2341 agggtcacgc cgaccccgat caggagcgac acgatgatcg tgcggggcag aacctgaagc
     2401 gagttcgcgg gaagctccgc ccccaccgcc ttgagcactg cgctcaggcc tgcggccagg
     2461 ccgtaaccaa ccacgagccc gagcagggat ccgaccaggc cgatgatcag accttcgatc
     2521 actaccgttc tcaggatctg gccacggctg gcccccaggg tacggagcat tccgaactca
     2581 cgggtccgct gggcgaccgt gatcgagaag acgttgaaga tcacgaacga accgacgaac
     2641 agcgcgattg ccgcgaaggc gagaagcgcg atggtcagga actggaggct gtcacggatc
     2701 tcgcttgcgt tccgatcggc gttttcctgc gcggtctcaa cccgcaggtt cttcggcacg
     2761 acctcggaga cccggtcgcg cagttcctcc gacgagatac cgctgtcggc ggcgatcgag
     2821 atctggtcgt attcgccgac ctttccggtc actcgctggg cgaccggcaa ctgaacctgg
     2881 gcaatcgagg aaccgccgaa ggaggcaccg ccaagctggg tgagaccgac gaccctgaag
     2941 ggaaccgcct tgtcggtgcc gaccagctcg atcgtgtcgc caagctcgat tcccttccgg
     3001 tcggcggcgg cttgatcgag ggtcgcctcg tgggcattgg tcggcgggtg accttcggga
     3061 taggtgacag cctcgaatac cttggggccg gtcgaggcaa tgaactgggg agagaaaccc
     3121 gcgccgatcg aatcgccctg gtcgtcgaga atcgcgccct gggtgaagat cgaaccggcg
     3181 gccactttca cgccgtcgac ctggtcgacc ttcttcagga tcttcgccgg gaaggcaggg
     3241 gtgctgccgt cctgctggtc aacctcggat ttcgccgtaa ccacctgtgc cgtgccggcc
     3301 agcgattcgt tgaagatctg gtcgaacgcc tggttggtgg tgtcggtcag gatgtaggtg
     3361 ccggcgacca gggcgatgcc gaagatcacc gcgaccgcgg tcataagcga cc
//
LOCUS       DAOQFI010000114         3408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13179_length_3408_cov_3.056964, whole genome shotgun sequence.
ACCESSION   DAOQFI010000114 DAOQFI010000000
VERSION     DAOQFI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13179_length_3408_cov_3.056964"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1237
                     /locus_tag="PLE93_02945"
     CDS             89..1237
                     /locus_tag="PLE93_02945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH86036.1"
                     /translation="MAKRATKRGDERTDLTGSRDVLICGASFAGLMVARQLAGTGADV
                     LVVDRYEIGERQTSACAIPTAWLEELGLIESEKQRFDSVLIHTAHGSIRLPVPWSFST
                     FDYPSFCEMLWAQCDAEFETAKVNGRAKTPEGDHVIDTDRGEITAPLVVDSLGWKRVL
                     AAGAHYQPPNQPLSRGLEVHPPGGNVDLEVFINRRYVPAGYGWSFPADDEVRVGVGSF
                     DPHFPVRPTTDLIADDLNVEKSAYQGNWIPHKLRPATGDGIFFAGDSAGHCFPLTAEG
                     IRPALYFGAALGMELRDVVEGRKSREEALTCYHDFSAGHQWAFDWLLRAQKLLPKVHP
                     RLVKPTLAASFKAGGSGDYAFNKYLDIAPPEFAALATRALKENSPVPT"
     gene            complement(1234..2745)
                     /locus_tag="PLE93_02950"
     CDS             complement(1234..2745)
                     /locus_tag="PLE93_02950"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNH86037.1"
                     /translation="MTVSTETETTTLQNYVGGKWVEAGGAELHDVVNPANGEVIARVP
                     FSTASHLDEAVRAARKALPAWRGVSTIARARMLFTLREKLEARKDEIARCVVTEMGKT
                     LPDATAEVGRMIEMVEAATAIPTTMQGKVLEDVARGVDAETIRQPVGVCGIIAPFNFP
                     AMVPFWFLPFAIACGNTIVLKPSEQVPMTQQKVFGIIDELDLPDGVVNLVNGSREVVE
                     GMLDHPDIDAISFVGSAPVAQLVYERAAQTGKRVQALGGAKNHMVICPDAVVEPTVNG
                     LIGSGFGAAGQRCMAGSVIVTVGSGHDSVVPKLVEETKKLHVGNGLDENIDVGPVISC
                     DARDRIDGWVAKAIEEGAEVLVDGRTPLPDAPEGSSYVGPTILDNVKPTDSIIENEVF
                     GPVIAIVNVDTLEEAIEVVNASPFGNGVSIFTESASAVRKFRHDVEVGMVGVNIGVAA
                     PVAFFPFSGWKASFLGDLHAHGPDGVEFFTRKKTITSRYFSGGHESGSYFVER"
     gene            complement(2864..3316)
                     /locus_tag="PLE93_02955"
     CDS             complement(2864..3316)
                     /locus_tag="PLE93_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNH86038.1"
                     /translation="MTKVRRFLAVLLVLALGAGVAACGSDSDSGSGEGSEASAVEGVT
                     WQAMNLVTQGAATSLPKGVKPPTLEINDGQASIFAGCNSGSGEATVNEATIDFGPIAL
                     TRKSCEQVQNQIEFLVTQVLQGEVKYEVNSDGNLILEKGTDSIVYTKG"
BASE COUNT          584 a         1149 c         1056 g          619 t
ORIGIN      
        1 atgttcaacc cgcccctccc attcgaacaa actgatggtt gggaggggct ctgacttctg
       61 tgccccggtt ttcattaccc tccaccggat ggcaaaacgg gcgacaaagc ggggggacga
      121 acgaaccgat ctgaccggat cgcgcgacgt tctcatctgt ggagccagtt ttgccggact
      181 gatggtggcc cgccagctgg ccggtaccgg cgcggacgtc ctggtcgtcg atcgctacga
      241 gatcggcgag cgccagacct ccgcctgtgc gatcccgact gcctggctgg aggagctggg
      301 gctgatcgaa tccgagaagc agcgatttga ctcggtcctc atccacaccg cccacggttc
      361 gatccgcctg ccggtcccct ggtccttctc cacctttgac tacccgagct tttgcgaaat
      421 gctctgggcc cagtgcgacg ccgagttcga aaccgccaag gtcaacggca gggccaagac
      481 ccctgaggga gaccatgtga tcgacaccga tcggggcgaa ataaccgctc cgctggtggt
      541 cgattcgctc ggctggaagc gggttctggc tgccggcgcc cactatcagc cgcccaacca
      601 gccgctttct cgcggcctgg aggtccaccc gccgggtggc aacgtcgacc tcgaagtctt
      661 catcaaccgg cgctacgttc ccgccggtta tggctggtct ttcccggccg acgacgaagt
      721 ccgggtcggg gtcggttcct tcgatcctca cttcccggtc cgcccgacca ccgacttgat
      781 cgccgacgac ctcaacgtcg aaaagtcggc ataccagggc aactggatcc cccacaaact
      841 ccggcccgcg accggcgacg gcatcttctt cgccggtgac tcggccggcc actgcttccc
      901 cctgaccgcc gagggcatcc gccccgccct ctatttcggt gccgcgctcg gcatggaact
      961 tcgcgatgta gtggaaggcc gcaagtcacg cgaagaggcc ctgacctgct accacgactt
     1021 cagcgccggc caccagtggg cctttgactg gctcctcagg gcccagaagc tcctgccgaa
     1081 ggtccacccc cgcctggtca aaccgaccct cgcggcctct ttcaaggccg gcggctcggg
     1141 tgactacgcc ttcaacaagt acctcgacat cgccccaccg gaattcgccg ccctcgccac
     1201 aagggccctc aaagaaaact cgcccgtacc cacctagcgt tctacgaagt aggagccgga
     1261 ttcgtggccg cccgagaaat agcggctcgt gatcgtcttc ttacgggtga agaactcgac
     1321 tccgtcgggg ccgtgggcgt ggaggtcacc gaggaaggat gccttccagc ccgagaaggg
     1381 gaagaaggca accggagcag ccacgccgat gttgacgccg accatgccga cctcgacgtc
     1441 gtggcggaac ttgcggaccg ccgaggcgga ttcggtgaag atcgaaacgc cgttgccgaa
     1501 gggactggcg ttgacgacct cgatcgcctc ctcgagagtg tcgacgttga cgatcgcgat
     1561 gaccgggccg aagacctcgt tctcgatgat cgaatcggtc ggtttgacgt tgtcgaggat
     1621 tgtcggcccc acgtaggacg agccctcggg ggcatcgggc agcggcgtgc gcccgtcgac
     1681 caggacctcc gccccttcct cgatcgcctt ggcgacccag ccgtcgatgc ggtcgcgagc
     1741 gtcgcaggaa atgaccgggc cgacgtcgat gttctcgtcg agcccgttgc cgacatggag
     1801 cttcttcgtt tcctcgacca gcttcggaac cacgctgtca tggcccgagc ccacggtgac
     1861 gatcaccgag ccggccatgc agcgctgacc ggcggcgccg aagccggagc cgatcagtcc
     1921 gttgacggtc ggttccacca ccgcgtcggg gcagatcacc atgtggttct tggccccgcc
     1981 gagagcctgg acgcgcttgc cggtctgggc cgcgcgctcg tagacgagct gggcgaccgg
     2041 ggccgatcca acgaaggaga tcgcgtcgat gtcggggtga tcgagcattc cctcgacgac
     2101 ctcgcggctg ccgtttacga ggttgaccac cccgtccgga aggtcgagct cgtcgatgat
     2161 tccgaagacc ttctgctggg tcatcggcac ctgctccgaa ggcttcagca cgatcgtgtt
     2221 gccgcaggcg atcgcgaagg gcaaaaacca gaacggcacc atggccggga aattgaaggg
     2281 agcgatgatg ccgcagaccc cgaccggctg gcggattgtt tccgcgtcca cgccgcgggc
     2341 cacgtcctcg agcactttgc cctgcatcgt ggtcgggatc gcggtggccg cctcgaccat
     2401 ctcgatcatc cggccgacct cggcggtggc atcgggcagg gtcttgccca tctcggtgac
     2461 cacgcagcga gcgatttcgt ccttgcgcgc ctcgagcttc tcgcgcaggg tgaagagcat
     2521 ccgggcccgg gcgatcgtgc ttacgccgcg ccacgccgga agagccttcc gggcggcgcg
     2581 aaccgcttcg tcgaggtgac tggcggtcga gaagggaacc cgggcgatga cctctccgtt
     2641 ggccggattg accacgtcgt gaagctcggc cccgccggct tcgacccact tgccgccaac
     2701 gtaattctga agcgtggtgg tttcggtctc ggtgctgacg gtcatacggc ctcctcgggg
     2761 aaaacgggtc tttctcaggc taacgcccgg agaccagacc gaaagtggac ataccgtgaa
     2821 gtctcaacgc caaactggac gccacgtcca gaacctggcc gggtcagccc ttcgtgtaga
     2881 cgatgctgtc ggtgcccttt tccaggatca ggttgccgtc gctgttgacc tcgtacttga
     2941 cctcgccctg aagcacctgg gtgaccagga actcgatctg gttctggacc tgttcgcagg
     3001 acttcctggt cagggcgatc ggcccgaagt cgatcgtggc ctcgtttacc gtcgcttcgc
     3061 ccgagccgct gttgcagccc gcgaagatgc tggcctggcc gtcgttgatc tcaagggtcg
     3121 gaggcttgac tcccttgggc aggctggtcg cggcgccctg ggttacgagg ttcatcgcct
     3181 gccaggtcac gccctcgacc gcggaagcct cggagccctc gcccgaccct gaatcggagt
     3241 ccgacccgca ggccgctacc ccggcaccca gcgccagtac caacagaacc gccagaaatc
     3301 tcctcacctt ggtcatgact gaagggtata tccgatcgtg atgggtacgg tcccaacgtg
     3361 acctcctcgc cggagcaggt tcagcgggtc tatttgaccc tgatgctg
//
LOCUS       DAOQFI010000115         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13206_length_3404_cov_2.523141, whole genome shotgun sequence.
ACCESSION   DAOQFI010000115 DAOQFI010000000
VERSION     DAOQFI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13206_length_3404_cov_2.523141"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..400)
                     /locus_tag="PLE93_02960"
     CDS             complement(65..400)
                     /locus_tag="PLE93_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNH86039.1"
                     /translation="MGIVVYEKPTCTTCRNLFRLLEAEGIDFESVEYHVTGLTEDEIR
                     GLLGKAGITAAEALRMREEGARELAAEDEDTIIAAMAERPELLQRPFVVNGERAVLAR
                     PVERALEVI"
     gene            complement(410..910)
                     /locus_tag="PLE93_02965"
     CDS             complement(410..910)
                     /locus_tag="PLE93_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNH86040.1"
                     /translation="MDVIDELVENNREFSSRVPDQDLDVKPSRQLTIVTCMDSRIDVY
                     QALGLKDGEAHILRNAGGVITDDVIRSLAISQRRLETRAIMLIHHTNCGMQTITDDGF
                     RSELQEATGTAPAFAIESFTDVEADVAQSIRRVRRSEFIPHRDNVRGFVYNVEDQTLT
                     EVTVED"
     gene            complement(956..1489)
                     /locus_tag="PLE93_02970"
     CDS             complement(956..1489)
                     /locus_tag="PLE93_02970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNH86041.1"
                     /translation="MDRKLGVLIGLIVLIGALVAVILIGRGSGDSGDSAASGEIPSEV
                     TAGVSEDLSERPKLAGTSETPPTALVKKDIVVGDGAEAKEGDTVKVQYVGALYDNGKE
                     FDASWDRNKEAFSFQLGGGQVIKGWDEGVAGMKVGGRRVLVIPPDLGYGPQGSPPTIP
                     GNSTLVFVVDLEDVQGQ"
     gene            1551..>3404
                     /locus_tag="PLE93_02975"
     CDS             1551..>3404
                     /locus_tag="PLE93_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNH86042.1"
                     /translation="MKSLLVLPAGPRARWVTVAVWFLAMFVVFGTNIPGKFADAENNE
                     SVSYLPGDAEATRALEAQKKLVGSEEAPMVIIYRREGGLTPADKARIKADVHELNEKV
                     EGVSKRFVITQRSKDGSAVIVTSSITSTGEGEDIREPVEAARDLVSGSFDGLQVDITG
                     PAGYASDSIKVFESINGTLFAAAFGLVFILLILIYRSPFLLWLPLIAVGFAEMGARAV
                     GYGLTELGVTVNGQSSSILSVLVLGAGTDYALLLISRYREELRHRESRTESMRVALAS
                     AGPAIVYSGLTVVAALLCLSLAELNSTASMGPIGAMGILMAMLSMLTLLPAMLVIAPR
                     FVFWPKVPRVGTGGADAEHGPWRRLGDRIAKRPRPVWITAVLVLLVMCAGLFSYNDNL
                     TSANGYVDEVEAVRGEESIAKAFPGGSNVPTEIVVGDPKKLDEVEAAVRKVDGVAGSQ
                     VAFRTPQGVVLNVILEDEPYSKSAFALIPAIRDAAHEAGGDTTLVGGGTAVEHDIREA
                     NRHDVKLIVPIVLLVVFLILVGLLRALAAPVMLIGTVVLSFFAALGIGFLVSDYIFGF
                     PGVDPSLPLYVFVDTRTMRVINVLVRPSSAQVTYAMSAALAYYDAYRTARLP"
BASE COUNT          548 a         1109 c         1113 g          634 t
ORIGIN      
        1 cgcgttccgg cgctacaccg gagcctggcc gggagaaacc ggcaaagaca actgaccgcc
       61 cgggtcagat gacctcgagc gccctttcaa cggggcgggc gaggacagcc ctttcgccgt
      121 tgacgacgaa ggggcgctgg aggagctccg gccgttcggc catcgcggcg atgatggtgt
      181 cttcatcttc ggcggccagc tcgcgggcgc cttcctcgcg catgcggagc gcctcggccg
      241 cggtgatgcc ggccttgccc agcaggccgc gaatctcgtc ctcggtcagg ccggttacgt
      301 ggtactcaac cgactcgaaa tcaatgccct cggcttccag cagcctgaag aggttgcggc
      361 aggtggtgca ggtcggcttc tcgtaaacga cgatgcccac ggtgtccggc tagtcctcga
      421 ccgtgacttc ggtcagggtc tgatcttcca cgttgtagac gaagccacgc acgttgtcgc
      481 ggtgggggat gaactccgag cggcgcaccc ggcggatcga ctgcgcgaca tcggcctcga
      541 cgtcggtgaa cgattcgatt gcgaaggcgg gtgcggtgcc ggtcgcttcc tgaagctcgg
      601 acctgaagcc gtcgtcggtg atcgtctgca tgccgcagtt ggtgtggtgg atcagcatga
      661 tcgccctggt ctccagcctg cgctgggaga tcgcgaggga ccggatcacg tcatcggtga
      721 tcaccccgcc ggcgttcctg aggatgtggg cctcgccgtc cttgaggccg agcgcctggt
      781 agacgtcgat tcgcgaatcc atgcaggtga cgatcgtcaa ctgccggctc ggctttacgt
      841 caaggtcctg gtcgggaacc cttgaagaga actcccggtt gttctcgacc agttcgtcga
      901 ttacgtccat tggttcaaac cttttgagcc gggaagcccc gattgaggcc gggtgttact
      961 ggccctggac gtcttcgagg tcaaccacga agaccagggt cgagttgccc ggaatggtcg
     1021 gagggctgcc ctgggggccg tagccgagat cgggcggaat gaccagcacg cgccggccgc
     1081 cgaccttcat gcccgcgaca ccttcatccc agcccttgat cacctggccg ccgccgagct
     1141 ggaagctgaa ggcttccttg ttgcggtccc aggaggcgtc gaattccttg ccgttgtcat
     1201 agagcgcacc gacatactgg accttgaccg tgtcgccttc cttcgcctcg gcgccgtcac
     1261 cgaccacgat gtccttcttg accagcgcgg tggggggcgt ctcgctggtg ccagccagct
     1321 tcggccgttc gctcagatcc tccgaaaccc cggcggtgac ttcactcggg atctcgcccg
     1381 aagcggccga atcacccgag tcgccgctgc cgcggccgat caggatgacg gcgacgagcg
     1441 cgccgatgag aacgatcagg ccaatgagaa ccccaagctt ccgatccatg ccgcgcatat
     1501 tacggtgaac ccggccgggt tgcctcgggc cggtcgctag ggtctctggc atgaagtctc
     1561 tcctcgtcct gccggccggg ccccgggccc ggtgggtaac agtcgctgtc tggttcctcg
     1621 cgatgttcgt cgtcttcggg acgaacattc ccggcaagtt cgccgacgcc gaaaacaacg
     1681 agtcggtgtc atatctcccc ggtgacgcgg aagccacccg agccctggaa gcgcaaaaga
     1741 agctggtcgg ctcggaggaa gcgccgatgg tgatcatcta ccggcgcgaa gggggcctca
     1801 ccccggccga caaggcccgg atcaaggccg atgttcatga actcaacgag aaggtcgagg
     1861 gagtcagcaa gaggttcgtc atcacccagc gatcgaagga cggctcggcg gtcatcgtca
     1921 cctcctcgat cacttccacc ggagagggag aagacatccg cgaaccggtc gaggccgcgc
     1981 gcgacctggt cagcggcagc ttcgacgggc tgcaggtcga catcaccggg ccggccggat
     2041 acgcctccga ttcgatcaag gtcttcgaat caatcaacgg gacccttttc gcggccgcct
     2101 tcggcctggt cttcatcctg ctgatcctga tctaccggtc accgttcctg ctctggctgc
     2161 cgctgatcgc ggtcggcttc gcggagatgg gggcccgggc ggtcggttac gggctgaccg
     2221 aactcggggt gaccgtcaac gggcagtctt cctcgatcct ttcggtcctt gtcctgggcg
     2281 ccggaaccga ttacgcgctc cttttgatca gtcgatatcg cgaggaactg agacaccggg
     2341 agagccggac cgagtcgatg cgggtcgccc tggccagtgc cggcccggcg atcgtctatt
     2401 ccggcctgac cgtggttgcc gccctgctct gcctttcgct ggccgagctc aactcgaccg
     2461 cgtcgatggg cccgatcggc gcgatgggga ttctgatggc gatgctctcg atgctgacgc
     2521 tcctgccggc gatgctggtg atcgcgcccc gcttcgtctt ctggccgaag gtgccgcggg
     2581 tcgggaccgg cggcgccgat gcggaacacg gcccgtggcg gcggctcggt gaccggatcg
     2641 cgaaacggcc caggccggtc tggatcaccg cggttctggt tctgctggtg atgtgcgcgg
     2701 ggctgttcag ctacaacgac aacctgacct cggccaacgg ctatgtggac gaggtcgaag
     2761 cggtgcgggg tgaggaatcg atcgccaaag ccttccccgg cggctccaac gttccgaccg
     2821 agatcgtggt cggtgatccg aagaagctcg acgaagtcga agccgcggtc cgcaaggttg
     2881 acggggtcgc cggctcccag gtcgcattca gaacccctca aggggtggtc ctgaacgtga
     2941 tcctcgaaga cgagccctac tcgaaatccg cctttgccct gatacccgcg atccgcgacg
     3001 ccgcccatga ggccggggga gacaccaccc tggtcggcgg cgggaccgcg gtcgaacacg
     3061 acatccgcga ggccaaccgg catgacgtca agctgatcgt tccgatcgtg ctgctggtcg
     3121 tcttcctgat tctggtcggc ctgctcaggg cgctggcggc gccggtgatg ctgatcggaa
     3181 ccgtcgtgct cagcttcttc gcggcgctgg gaatcggttt cctggtcagc gactacatct
     3241 tcggattccc cggggtcgac ccctcgctgc cgctctacgt cttcgtcgac acgcgcacga
     3301 tgcgagtgat caacgtgctc gtgcgtccgt cgagcgcgca ggtcacctac gcgatgagcg
     3361 ccgcgctcgc gtactacgac gcgtaccgca ccgcgcgcct cccc
//
LOCUS       DAOQFI010000116         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13215_length_3403_cov_2.794504, whole genome shotgun sequence.
ACCESSION   DAOQFI010000116 DAOQFI010000000
VERSION     DAOQFI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13215_length_3403_cov_2.794504"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2366)
                     /locus_tag="PLE93_02980"
     CDS             complement(<1..2366)
                     /locus_tag="PLE93_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH86043.1"
                     /translation="MNRVFAVLAFVVAALQIGVAPASADPAPDLPAGPVLATDDWPND
                     PNFARCELQDPVSGCQDSEQWNLYGPMDGLCLAPGGAVFDQPRPDGGLPCWARNATDP
                     ESSSGINMTGAWAQGNIGRDDVLVAYVEGGVNYSANGIKDGLDSIYLNAGELPYPQGW
                     DGQDLGRYDFDGNGEFDIRDYAKDPRVNPACVSPTTQFTTYQEGTTRGCADDGTHEYL
                     HSVNIGGPLTPYLSPEDLIAVFGHCKITDHRIESCPADARIDNDGNGYPNDVSGWNAY
                     QNTNDPQSEDFNYGHAPGLIGEMGGEADNGYAGVGNCRNCRVVPIKSGAESVGKADGL
                     APAITYAADINVTAMSTVVVSYAYSSALKAAVKHAVDKGILMSMDSNDFDSMDHTDGH
                     LFDDVFPGNGLAQDIEGKNAQTFRARSNVTSYGTHSIFSGGESTTSGATPFQAAFLAM
                     TQSAALNAVDENIIPRKLTPNEIKQVLINTASEILPQRDTPDVANQWPGNPDSKTDST
                     HTAWSTQYGYGRVNIGKATKLIMDGRVPPTADIDSPSWYKYVDPTKGGKLQVKGKVAP
                     SAWGSEGLEWTLEWALGANPSDDDFKALSTGKAAKSGVLGTLDLKDIPASYAKAAPAE
                     NVMPPDGPEQYTVTFRLRARDGNGLKGEDRRSIGARADKDLVNGFPKAIGTEMSAAPS
                     FVDLGGKRQLELVFGTMDGSVSALQPDGSQAPGFPVYTWKLKDIDPRNPQNFDSEAYS
                     SVQALRDARDPISGIAVGDLDGNGAQSIVATTTSGWVYVWNSSGKLREG"
     gene            complement(2392..>3403)
                     /locus_tag="PLE93_02985"
     CDS             complement(2392..>3403)
                     /locus_tag="PLE93_02985"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNH86044.1"
                     /translation="GGARRMAGATGLDQAMENAVEEAIVRAIESEAVERSMTRIINGP
                     LIENAVRDAMKSPAVEQALMEVVDSELVDRLWARILDSNETQRLIERIAEAPEVRAAI
                     ASQSLGLVDDLGRGAARVTRAIDFALERLARRIFFRSRREVPTDRVGVATRALALVID
                     GVAVNLVMISSTAVLGMIASVLGIDLGNLGGAAYAVGGAVWLGLGSLYLFAFWAFSGQ
                     TLGMRFFDIRIEHEGKWVIGPRRAFRRLVGFWLSAIPFCLGFLGVLLRLDRRGFHDRL
                     GQTMVYYINPAGPNQPHLEPVLQPGMDLMDDLVPDEPLISAEKPIDSPQGLPREVRGP
                     GK"
BASE COUNT          540 a         1071 c         1166 g          626 t
ORIGIN      
        1 ccttctctca gcttgccgga ggagttccag acgtagaccc agccgctggt cgtggtggcg
       61 acgatcgact gggcgccgtt gccatcgaga tcaccgaccg cgatgccgct gatcggatcc
      121 cgcgcgtccc ggagcgcctg aaccgacgag taggcctcac tgtcgaaatt ctgcgggttg
      181 cgcggatcga tgtccttcag cttccaggtg tagacgggga agcccggagc ctgcgaaccg
      241 tccggctgca gagcgctgac cgagccgtcc atggtgccga agacgagctc gagctgacgc
      301 ttgcctccga ggtcgacgaa gctcggcgcc gcgctcattt cggtgccgat cgccttcggg
      361 aacccgttga ccaggtcctt gtcggcgcgg gcgccgatcg agcggcggtc ctcgcccttg
      421 aggccgttgc cgtcacgggc ccggagccgg aaggtcaccg tgtactgctc ggggccgtcc
      481 gggggcatca cgttttcggc cggagccgcc ttggcgtagg aggccgggat gtccttcagg
      541 tcgagcgtgc cgagcacacc ggacttggcg gccttgccgg ttgaaagcgc cttgaaatca
      601 tcatcggagg gattggcgcc gagggcccat tcgagggtcc attcgagccc ttccgaaccc
      661 caggcggaag gtgcgacctt gcccttcacc tggagcttgc cgcccttggt cggatcaacg
      721 tacttgtacc aggagggcga atcgatgtcg gcggtcgggg gaacccggcc gtccatgatc
      781 agcttggtcg ccttgccgat gttgacccgg ccgtagccgt actgggtcga ccaggcggtg
      841 tgggttgaat cggtcttcga gtccgggttt ccgggccact ggttggccac gtccggggtg
      901 tcccgctggg ggaggatctc gctggccgtg ttgatcagca cctgcttgat ctcgttcggg
      961 gtcagcttgc gggggatgat gttttcgtcg accgcgttca gggcggctga ctgggtcatc
     1021 gcgaggaatg ccgcctggaa gggggtggcg cccgaagtcg tggactcgcc tccggagaag
     1081 atcgagtggg tgccgtaact ggtcacgttc gagcgggccc ggaaggtctg ggcgttcttc
     1141 ccttcgatgt cctgggcgag cccgttgccg gggaagacgt catcgaagag atggccgtcg
     1201 gtgtggtcca tcgagtcgaa gtcgtttgag tccatcgaca tcaggatgcc cttgtcaacc
     1261 gcatgcttga ccgcggcctt gagcgcgctc gaataggcgt aggacaccac cacggtggac
     1321 atcgccgtca cgttgatgtc agctgcgtag gtgatcgccg gagcgagacc gtccgccttg
     1381 ccgacagatt cggccccgga tttgatcggg accacgcggc agttgcggca gttgccgacg
     1441 ccggcgtagc cattgtctgc ctcgccaccc atctcgccga tcaggccggg cgcgtgaccg
     1501 tagttgaaat cttcggactg ggggtcgttg gtgttctggt aggcgttcca gcccgaaacg
     1561 tcgttcgggt agccgttgcc gtcgttgtcg atcctggcgt cagccgggca ggattcgatc
     1621 cggtggtcgg tgatcttgca atggccgaac acagcgatca ggtcttcggg ggacagatag
     1681 ggagtgagcg gaccgccgat gttgaccgag tggaggtact cgtgggtgcc gtcgtcggcg
     1741 cagccgcggg tcgtgccctc ctggtaggtg gtgaactggg tggtcgggga aacgcaggcg
     1801 gggttgaccc gcgggtcctt ggcgtagtcg cggatgtcga actcgccgtt accgtcgaag
     1861 tcatagcggc cgaggtcctg gccgtcccag ccctgggggt agggaagctc gccggcgttg
     1921 aggtagatcg agtcgagtcc gtccttgatc ccgttggcgc tgtagttgac cccgccctcg
     1981 acgtaggcga ccaggacgtc gtcacgaccg atgttgccct gggcccaggc gccggtcatg
     2041 ttgatgccgg aagagctttc gggatcggtc gcgttgcggg cccagcaagg gaggccgccg
     2101 tccgggcggg gctggtcgaa cacggcgccg ccgggggcga ggcataggcc gtccatcggt
     2161 ccgtacagat tccactgctc gctgtcctgg cagccggaaa ccggatcctg cagttcgcag
     2221 cgcgcgaagt tcgggtcatt cggccagtcg tccgtcgcca gcactggtcc ggctggcagg
     2281 tcgggtgcgg ggtcggccga cgcgggagcg accccgatct gcaaagcggc caccacaaag
     2341 gcaagcacgg caaagacgcg gttcacgaac aagacattcc tcccttggta ctcatttccc
     2401 tggtcccctc acctcccgag gcagaccctg cggggaatct atcggctttt cggccgaaat
     2461 cagtggttcg tcaggtacca ggtcatccat caggtccatc cctggttgca aaaccggctc
     2521 gaggtggggt tgattcggtc cggccgggtt tatgtagtag accatcgtct ggcccagccg
     2581 gtcgtggaat ccgcgccggt caaggcgcag cagaaccccg agaaaaccga ggcagaaggg
     2641 aatcgccgac agccagaagc cgaccagtct cctgaaagcc cgtcgcggac cgatcaccca
     2701 ctttccctcg tgttcgatcc ggatgtcgaa gaaccgcatg ccgagcgtct gtccggaaaa
     2761 cgcccagaag gcgaagagat agagcgaccc gagccccagc cacacggcgc cgccaaccgc
     2821 gtaggcggcg ccgccgaggt tgcccaggtc gattcccagg accgaagcga tcatcccgag
     2881 cacggcggtc gaggaaatca tcaccaggtt gactgcgact ccgtcgatca ccagtgcgag
     2941 ggcgcgagtt gcgacgccga cccgatcggt cggaacctcc cgcctcgacc ggaagaagat
     3001 gcggcgggcg agccgctcga gggcgaagtc gatcgcccgg gtgacccgcg cggcgccgcg
     3061 gccgaggtca tcgaccagtc cgaggctctg cgaggcgatc gcggcccgga cctccggcgc
     3121 ctccgcgatt cgctcgatca gtcgctgggt ttcgttgctg tcgaggatcc gggcccagag
     3181 ccggtcaacc agttcggaat cgaccacctc catcaaggcc tgctcgactg ccgggctctt
     3241 catcgcatcc cgcacggcgt tttcgatcag gggcccgttg atgatccggg tcatcgagcg
     3301 ctccacggcc tcgctttcga tcgcccggac gatcgcctcc tcgaccgcgt tttccatggc
     3361 ctggtcgagt ccggtcgccc cggccatccg cctggccccg ccg
//
LOCUS       DAOQFI010000117         3402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13222_length_3402_cov_2.970122, whole genome shotgun sequence.
ACCESSION   DAOQFI010000117 DAOQFI010000000
VERSION     DAOQFI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13222_length_3402_cov_2.970122"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="PLE93_02990"
     CDS             complement(<1..1675)
                     /locus_tag="PLE93_02990"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HNH86045.1"
                     /translation="MGSGRKFSAVVSTIAASFFMLVMAGPASADLSQIPTATVTGPIA
                     VTADSHPFLATDIDLDKYGYVENEYFLAGDAYRYDMTGAIDTPAAKITTGGSADDGKY
                     PFKTRIVVRRPANPADANGVVIAEWNNVTSTQDVEFNWFGDPYFLLKHGYTFVGVTAQ
                     NVGVASLKAFDSDRYGTLTVNGNDTVPTGTGLDADALSYDVYSSVVKALKGSRNGVDP
                     LGGITPSMVIASGESQSCGRLVTHYNRIEPIHEVVDAYLLTVCTSALRGDRGEKAIRI
                     ITETENRVPRTVASDPDTGSIRHWEVAAASHLPRVAFDNLNPVFSRDFATVTANCVKF
                     PLSRIKWPYTQNAAISDLVGWVGDDAAPPIAPRGEYQASPPDMANQLERDENGIALGA
                     IRYPDVTVPTATNDGINSAAPGGSIFSAFCGLFGSSVPFSPELLHSLYTDQADYLAQY
                     SEAADALADSGFILPEDADRLKGDSREYARLRPSLPSVIGGTSNKGGFQLVWVATEAP
                     DTTFEVQRASTKKPNAWAKVKTKSVSDSTATIADAAQGTAYFRVNSATVL"
     gene            complement(1731..2765)
                     /gene="thrC"
                     /locus_tag="PLE93_02995"
     CDS             complement(1731..2765)
                     /gene="thrC"
                     /locus_tag="PLE93_02995"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNH86046.1"
                     /translation="MPQPLIERYRDALPLDPGDPVVSLNEGSTPLVEAPRLSERVGAR
                     VWLKVEGANPTGSFKDRGMTVAVSRAAGRGAKAVICASTGNTAASASAYAVRAGLRGA
                     VIVPEGKIAIGKLAQSLMHGARVVALEGNFDDALTIVRQLAERNPIELVNSVNPYRIE
                     GQKTAAFELIDELGQAPDALAIPVGNAGNVTAYWKGFQERGTAPICYGFQAAGSAPLV
                     DGAPVAHPETVASAIRIGNPARWEEAMNAFNSSRGRVAAVSDEQILDAYRWLAANEGV
                     FCEPASAASVAGILNHGLPTVEGMEKPETVVCVLTGHGLKDPDTALGKAPSVINCPNE
                     IGAVEEAVFG"
     gene            complement(2772..>3402)
                     /locus_tag="PLE93_03000"
     CDS             complement(2772..>3402)
                     /locus_tag="PLE93_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNH86047.1"
                     /translation="HTPVTLDQVDFEGIEDIQPDDLAYAKELGLSLKLLGVAEKLEQG
                     ITVRVFPCFLYPGHPLAPIEGPFNAVMVEAPEITEVTMSGPGAGGLQTAAAVLGDVVS
                     VAAGDARVHTADRDLPVLADVTSSFYLHLEVADEPGVLAEVAKVLSDGGVSVKSVVQR
                     GLGDDARLVMVIHRCLESEFKAAVAQIAELDSLKSGPRFIRVIEEEFTG"
BASE COUNT          572 a         1087 c         1153 g          590 t
ORIGIN      
        1 gcagcacggt ggcgctgttc acccggaagt aggcggttcc ctgggcggca tccgcaatgg
       61 tcgcggtgct gtcgctcacc gacttcgtct tgacctttgc ccaggcgttg ggctttttgg
      121 tgctggcccg ctgaacctcg aaggtcgtgt cgggggcttc ggtcgcgacc cagacgagct
      181 ggaagccgcc cttgttcgat gttccgccga tcacgctcgg cagcgacggc cggagccgcg
      241 cgtattcacg tgagtccccc ttcaaccggt cggcatcctc gggcaggatg aagccggaat
      301 cggccagggc gtcggccgcc tcggagtact gcgccaggta gtcggcctga tcggtgtaaa
      361 gcgaatgaag cagctcgggc gagaacggga ccgatgaacc gaacagcccg cagaaggcag
      421 agaagattga gcctccggga gccgccgagt tgatgccgtc attggtcgcg gtgggcaccg
      481 tcacgtcggg gtagcggatt gcgcccagcg cgatcccgtt ctcgtcgcgt tccagctggt
      541 tggccatgtc cgggggactc gcctggtact cgccgcgtgg cgcgatcggc ggtgcggcgt
      601 cgtcacccac ccagccgacc aggtcgctga tcgcggcgtt ctgggtgtag ggccacttga
      661 ttcgcgacag cgggaacttg acgcagttcg ccgtcacggt ggcgaagtcg cgcgagaaca
      721 ccgggttcag attgtcgaag gcgacccggg gcaggtggga cgccgccgcg acctcccagt
      781 ggcgaatcga accggtgtcc ggatcggacg cgacggttcg cggaacccgg ttctcggtct
      841 cggtgatgat ccggatcgcc ttctccccgc ggtcgccgcg gagcgcggag gtgcagaccg
      901 tcagcagata ggcatccacg acttcatgaa tcggctcgat ccggttgtaa tgggtgacca
      961 gacgaccgca ggactgtgat tcacccgagg cgatcaccat cgagggcgtg atcccgccca
     1021 ggggatcgac gccgttgcgg ctgcccttca gtgccttgac gaccgaggag tagacgtcat
     1081 atgagagggc gtcggcatcc aggccggtac cggtcggcac ggtgtcgttg ccgttgaccg
     1141 tcagcgtccc gtaacggtcg ctgtcgaagg ccttcaggga ggcgacaccg acgttctggg
     1201 cggtcacccc gacgaaggtg taaccgtgct tgagcaggaa gtacgggtca ccgaaccagt
     1261 tgaactcgac gtcctgggtc gaggtgacgt tgttccattc ggcgatgacc acgccgttcg
     1321 cgtcggccgg attggcgggg cgcctgacca cgatccgggt cttgaacggg tacttgccgt
     1381 catcggccga accaccggtc gtgatctttg ccgcgggagt gtcgatcgct ccggtcatgt
     1441 cgtaccggta ggcgtcaccg gcgaggaaat actcgttctc gacgtagccg tacttgtcaa
     1501 ggtcgatgtc ggtggccaga aacggatgtg aatcggcggt caccgcgatc gggccggtca
     1561 ccgtcgcggt cggaatctgg ctgagatcag ccgacgccgg accggccata accagcatga
     1621 aaaatgacgc cgcaatcgtc gagacgacgg ctgagaactt cctgcctgaa cccatttacc
     1681 tctccctctc ccgttgaaaa agctacggga gaatctaccg cagttggcgc ctagccaaag
     1741 acagcttctt cgaccgcgcc gatttcgttc gggcagttga tcaccgaggg ggccttgccg
     1801 agagcggtgt ccggatcctt gaggccgtgg ccggtgagta cgcagacaac cgtctcgggc
     1861 ttctccattc cctccacggt cggcaggccg tggttgagaa ttccggccac cgaagcggcc
     1921 gaggccggtt cgcagaacac accttcgttg gcggcaagcc agcggtaggc gtcgaggatc
     1981 tgctcgtcgc tgaccgcggc gacccgtccc ctcgaggagt tgaaggcgtt catcgcctct
     2041 tcccagcggg ccgggttgcc gatccggatc gccgaagcga ccgtctccgg gtgggcgacc
     2101 ggggcgccgt caaccagcgg agccgagccg gcggcctgga agccgtaaca gatcggtgcg
     2161 gttccccttt cctggaagcc cttccagtag gcggtcacgt tgccggcgtt tccgaccggg
     2221 atcgccagcg cgtccggggc ctggccaagc tcgtcgatga gctcgaacgc ggccgtcttc
     2281 tgaccttcga tcctgtacgg gttgaccgag ttgaccagct cgatcggatt ccgttcggcc
     2341 agctgccgga cgatggtcag ggcatcatcg aagttgcctt cgagggcgac cacccgggcc
     2401 ccgtgcatga gcgattgggc cagcttgccg atcgcgatct tgccttcggg gacgatgacc
     2461 gcgccgcgca gccccgcccg caccgcgtag gcggaggcgg aagcggcggt gttgccggtc
     2521 gaggcacaga tcacggcctt ggcaccacgc ccggccgccc gggaaaccgc cacggtcatg
     2581 ccgcgatcct tgaaggagcc ggtcgggttc gcaccttcga ccttcagcca gacccgggcc
     2641 ccgacccgct cggaaaggcg cggcgcctcg accagcgggg tcgagccctc gttcaggctg
     2701 acgaccggat cacccggatc cagcggcagg gcgtcgcggt acctctcgat aagtggctgt
     2761 ggcatggctt cctaacctgt gaattcttct tcgatgaccc ggatgaagcg cggccccgac
     2821 ttgagcgagt cgagctccgc gatctgcgcg accgccgcct tgaattccga ttcgaggcaa
     2881 cggtggatga ccatcaccag gcgggcgtcg tcgccgagcc cgcgctggac gacgctcttg
     2941 actgacacgc caccgtcgga aagcaccttg gcgacttcag ccaggacccc cggttcgtcg
     3001 gcaacctcga ggtggaggta aaaggatgaa gtgacgtcgg ccagcaccgg caggtcgcgg
     3061 tcggcggtgt ggacgcgggc gtcgccggcc gcgaccgaaa ccacgtcacc gagcacggcg
     3121 gcagcggtct gaagaccacc ggcgccgggg ccggacatgg tcacctcggt gatttcgggc
     3181 gcctccacca tcaccgcgtt gaacggaccc tcgatcggcg ccaggggatg gcccgggtaa
     3241 aggaagcagg gaaagacccg gacggtgatc ccctgctcca gcttctcggc gaccccgagc
     3301 agcttcagcg aaaggccgag ctccttggcg taggcgagat catccggctg gatgtcctcg
     3361 atcccctcga agtcgacctg atcaagggtg accggggtgt ga
//
LOCUS       DAOQFI010000118         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13455_length_3368_cov_3.359493, whole genome shotgun sequence.
ACCESSION   DAOQFI010000118 DAOQFI010000000
VERSION     DAOQFI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13455_length_3368_cov_3.359493"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1176
                     /gene="wecB"
                     /locus_tag="PLE93_03005"
     CDS             97..1176
                     /gene="wecB"
                     /locus_tag="PLE93_03005"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNH86048.1"
                     /translation="MRIVHVLGTRPNFVKMAPLIAAISRRSPETAQLIVHTGQHYDHL
                     MSEIFFDQLGVPAPDFMLEVGSGSHALQTARVMERLEPLLIENRPDLVVVPGDVNSTV
                     AAALTAVKLEIPVAHLEAGLRSYDRSMPEEINRIITDQIADTLWVHSEEALRNLELEG
                     IPAGRAGFVGNTMIDSLVALEDRFRPLETCRSFGLEPGSYLLVTLHRPALVDGPLLFD
                     AIAALNQVSAVMPVLFPVHPRTRARLAGNAELAENLHLVDPAGYLEFLSLEADAGAVL
                     TDSGGIQEETTYLGIPCFTLRDNTERPVTIELGTNVLLGLDPARITEIPAIIAGADPS
                     AAPPEPPPLWDGHAADRIAEQLAAT"
     gene            complement(1441..2277)
                     /gene="infC"
                     /locus_tag="PLE93_03010"
     CDS             complement(1441..2277)
                     /gene="infC"
                     /locus_tag="PLE93_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNH86049.1"
                     /translation="MPIPRRFDRRPPDRDTTRINERIRVPKVRLIGADGEQVGIVDTD
                     QARQMAREAELDLVEVAANAKPPVCRLLDYSKYKYEQEQKQKAARKHQTQVNIREIKL
                     RPKIADNDYNTKKGHVERFLKHGDKVKVTIMFRGREQAHPERGRDLLYRLFEDLGEEL
                     AVIESRPLQEGRNMSMLLGPSKEATREAEADKQARKKAKGKNRGGEAPEGPDHPNAVA
                     PDAVPDHPNAVAPDAIPDHPNAVAPDAEAPEPTEPEDPPAGPEAADEAAAPAEEPAET
                     SD"
     gene            complement(2371..>3368)
                     /gene="thrS"
                     /locus_tag="PLE93_03015"
     CDS             complement(2371..>3368)
                     /gene="thrS"
                     /locus_tag="PLE93_03015"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNH86050.1"
                     /translation="DVDLWHRSGHWDNYKDDMYFMEVDERRYALRPMNCPGACLVFGS
                     TRHSYRDLPLRLAEFGRVTRNEREGVLHGLLRVRAFTQDDAHVYCTIDQVETEVSEIC
                     EAIDELYARFGFENVEVELSTRPEKSLGSDELWEKAESTLKSALDSQNREYRINPGDG
                     AFYGPKIDFHVTDALGRSWQLGTCQLDFQMPERFELHYTDSEDGQERPVMIHRALLGS
                     MERFAGILIEHYAGRFPAWLAPVQARIVPVSDKHLDYARQVAEDLKAEGARVSVEERS
                     ESVGKKIRAAELGRFPYMLVVGDREMEAGEVSVRSHEDGELGSMAVEKFCMLLDS"
BASE COUNT          534 a         1175 c         1051 g          608 t
ORIGIN      
        1 gtcgcctttc ccccccggat acgggatgaa tcccagcgcc aatcggctct gactcgcacc
       61 gcccccggcc gccggcgccg atactatggc cccctgatgc ggatcgtcca cgtgctcgga
      121 acccggccca acttcgtcaa gatggcgccg ctgattgccg cgatttcccg gcgctcgccg
      181 gagaccgcgc aactgatcgt ccacactggc cagcattacg accacctgat gagcgagatc
      241 ttcttcgatc agctcggagt cccggctccg gacttcatgc tcgaggtcgg ctccggcagc
      301 cacgcgctgc agaccgcgcg ggtgatggaa cggctggaac ccctgctgat cgagaaccgg
      361 cccgacctcg tcgtggtccc cggggacgtc aattcgaccg tggccgcagc gctcaccgcg
      421 gtgaaactgg agatcccggt cgcccacctc gaggccggcc ttcgcagcta tgaccgcagc
      481 atgccggagg agatcaaccg gatcatcaca gaccagatcg ccgacaccct ctgggtccat
      541 tcggaggagg cgctgcgcaa cctggagctg gaaggcatcc cggccgggcg ggccggtttc
      601 gtcggcaaca cgatgatcga cagcctggtc gcactcgagg accgcttccg cccgctggaa
      661 acctgccggt ccttcggtct cgaaccaggc tcctacctgc tggtcaccct ccaccgcccc
      721 gccctcgtgg acgggccgct cctctttgat gcgatcgccg ccctcaacca ggtctccgcg
      781 gtgatgccag tgctcttccc ggtccacccc cgcacccggg cccggctggc gggcaacgcc
      841 gaactggcgg agaacctcca cctggtcgac cccgccggtt acctcgagtt cctctcgctg
      901 gaagccgacg ccggtgccgt actgaccgat tccggcggaa tccaggagga gacgacctac
      961 ctcggcatcc cctgcttcac cctgcgcgac aacaccgagc gcccggtcac gatcgagctc
     1021 ggcaccaacg tcctgctcgg cctcgacccc gcccggatca ccgagatccc ggcaatcatc
     1081 gccggagcgg atccgtcggc tgcgcctcct gaaccgccac ctctatggga cggccacgcg
     1141 gccgaccgca tcgcggaaca gctggcggct acctgaaact tcggcagtca ccaatccccc
     1201 tagacgggac ttcgcgtaga tcaggcagcg actgctgtct gacgaaccga gtccagcact
     1261 tgaatcagct catccaagtc ggcagctccc cagggtgacc aaccgacaaa cgagaaggcg
     1321 attctggcca catagcgctc cttttggcca tctcgattcc gaattgcgat gaactgtcgg
     1381 ctactcggcc agagacgcgg ccacgtcagc cgcttcagac tcgattggct tcgaactgaa
     1441 tcagtccgag gtttcggccg gttcttcggc cggggccgct gcttcgtcag cggcttcggg
     1501 cccggccggc ggatcttccg gctcggtcgg ctcgggagct tcggcgtccg gggcgaccgc
     1561 gttcgggtgg tccgggatcg cgtccggggc gaccgcgttg gggtgatccg gaaccgcgtc
     1621 cggagccacc gcgttggggt ggtcgggacc ctcgggggct tccccgccgc ggttcttgcc
     1681 cttggccttc ttcctggcct gcttgtccgc ctcggcctcg cgggtcgctt ccttggaggg
     1741 gccgagcagc atgctcatgt tgcgcccttc ctggagcggc ctcgattcga tcacggcgag
     1801 ctcctcgccg agatcctcga aaagtctgta aagcaggtcg cggccccgtt ccggatgggc
     1861 ctgttcgcgg ccgcggaaca tgatcgtgac cttgaccttg tccccgtgct tgaggaaacg
     1921 ctcgacgtga cccttcttcg tgttgtagtc gttgtcggcg atcttcggac gcagcttgat
     1981 ctcgcggatg ttgacctggg tctggtgctt gcgggcggcc ttctgcttct gctcctgctc
     2041 gtacttgtac ttcgagtaat cgagcagccg gcagaccggc ggcttcgcat ttgccgcgac
     2101 ctcgaccaga tcgagttccg cctcgcgggc catctgcctc gcctggtcgg tatcgacgat
     2161 tcccacctgc tcgccgtcgg cgccgatcag ccgcaccttg ggcacgcgga tgcgctcgtt
     2221 gatacgagtc gtgtcccgat cggggggacg ccggtcaaac ctgcgcggaa ttggcactag
     2281 ctaagaacca aggggcaggt caagcggaca agaaacgggt aggcgagcac gcgggcgcag
     2341 cggggcgcga gttctttttc gtgcggcgaa tcaactgtca agcagcatac agaacttttc
     2401 gacagccatc gagccgagct ccccgtcctc atgcgagcga accgagacct cgccggcctc
     2461 catctcccgg tcaccgacca ccagcatgta cgggaacctg cccagctcgg ccgcgcgaat
     2521 cttcttgccg accgattcgg agcgctcctc gaccgagacc ctggctccct ccgctttgag
     2581 gtcctcggcg acctggcggg cgtagtcgag gtgcttgtcg gagaccggga cgatccgggc
     2641 ctggaccggg gccagccagg ccggaaaccg gcccgcgtag tgctcgatca ggattccggc
     2701 gaaacgctcc atcgagccga gcagcgcgcg atggatcatc accggccgct cctgcccgtc
     2761 ttcggagtcg gtgtagtgaa gctcgaagcg ctccggcatc tggaagtcga gctggcaggt
     2821 gccgagctgc caggagcggc ccagggcatc ggtcacgtgg aagtcgatct tcggcccgta
     2881 gaaggcgccg tcgccggggt tgatccggta ctcgcggttc tggctgtcaa gcgcgctctt
     2941 cagggtcgat tccgctttct cccagagctc gtcgctgccg agcgacttct cgggacgggt
     3001 cgataactcc acctcgacgt tctcgaaccc gaagcgggcg tagagctcgt cgatcgcctc
     3061 gcagatttcc gacacctcgg tctcgacctg gtcgatcgtg cagtacacgt gcgcgtcatc
     3121 ctgggtgaag gcgcggaccc tgagcaggcc gtggagcact ccctcccgct cgttccgggt
     3181 cacccggccg aactcggcca gccgcagcgg caggtcacgg taggaatgcc gggtcgaacc
     3241 gaagacgagg caggcgcccg ggcagttcat cggccgcagc gcgtaacgac gctcgtcgac
     3301 ctccatgaag tacatgtcgt ccttgtagtt gtcccagtgg ccggagcggt gccagaggtc
     3361 gacgtcca
//
LOCUS       DAOQFI010000119         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13467_length_3366_cov_3.661130, whole genome shotgun sequence.
ACCESSION   DAOQFI010000119 DAOQFI010000000
VERSION     DAOQFI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13467_length_3366_cov_3.661130"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..845
                     /gene="pdxH"
                     /locus_tag="PLE93_03020"
     CDS             216..845
                     /gene="pdxH"
                     /locus_tag="PLE93_03020"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNH86051.1"
                     /translation="MSFPSPDRQPLARTDLDPDPIKQFGAWFEAAAEIVALPEAMTLA
                     TVDEAGRPDARMVLLKGHGADGFRFFTNYEGVKAAQLDASGEAALVFFWAPQDRQVRV
                     RGPVRRLDADASDRYFASRDRASQVGAWASPQSQPIPDQREALEELAEAAADRFAEGE
                     VPRPQHWGGFILEPRSIEFWQGRGARLHDRFRYDRQDSGEGWKITRLAP"
     gene            975..1643
                     /locus_tag="PLE93_03025"
     CDS             975..1643
                     /locus_tag="PLE93_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86052.1"
                     /translation="MAFFAALAAVLLVPSAAEAQTWSGGGITLPGPTTNPNIGKILPL
                     ARREFSRNVVERRGNNVPRYRNGKGRIAPYSISDQWCAAFSTWIWNHAGFKSYLGTSI
                     LWPSYDGTMVGVQVRDLTNWATRNGYFSYRAKPGYLVAYGTTHIGIVEKIDRTTGRAV
                     GSIEGNIGNRVNRVTVPMDRVTGYISPVRLTPIQLDKSAAYADMAVPPAALERAEASG
                     FSQP"
     gene            complement(1630..2085)
                     /locus_tag="PLE93_03030"
     CDS             complement(1630..2085)
                     /locus_tag="PLE93_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86053.1"
                     /translation="MDGVSEDFSGLPEYKTRSERNAERVDRRFGQRRNNPYRGHRTRI
                     ILVGGFAVALVLLALSLVVLFAPTPYLGTNYASIAKSVGAASAHDCRPGGSGWVCTKE
                     VDGSAARYEVKVDWAGCWDGRLIGKPAGSNGAQPEISGCVSLMDHLTAG"
     gene            complement(2156..>3366)
                     /locus_tag="PLE93_03035"
     CDS             complement(2156..>3366)
                     /locus_tag="PLE93_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86054.1"
                     /translation="TGYLPDSNLTDSAQRRDHTYLSLGTIAQGSRYGYGIAVASFGRN
                     GRLETKFGDRGRLVLTQPAARFDPKPKMTLLPSGGAMICAKDGRDSVVWRISRYGHLV
                     KSFGDDGRLTLDFSPRCQDISFDGTGAVISAMDIVEDRRGVDLTRVLPDGQVDRSYGV
                     DGVAQRPEMADLDFYWVYSSSLATDRRGRTLFIASSGDARYIVRYTKNGFPDKSFGIG
                     GKLHYGTDSVSQEKDGQKPMYLPGLTKTNGVVLGRDGSIYLTGLYSKKPFVAKLNSRG
                     FPVRRYGKGGVVILPQANTSQKPYYRKPFPARPYGIGVRGDGSVVVTGISRPACAENW
                     GCSRPLLVKRVEPNGKVDRAFAKRAFLSLGNRPNAIGNGVYFDGRRTVIAGSMKFASE
                     RDNFMLAGFR"
BASE COUNT          586 a         1109 c         1097 g          574 t
ORIGIN      
        1 ggggccattc cggacgcaag gtcctcggcg tcaggcgccc gttccgggtc aaggtccgga
       61 agatggccaa ggccgggaaa cggatcgtcg tgcgacagaa gatcaggtcc aaacgaggcc
      121 gcaagcacgc gattcgatac cagcgccttc gcgtggtgat ccgcacccgc aagtagaggc
      181 aaatggcacg ggccgggccc cggtaggctt ggcccgtgtc ttttccctcg ccggaccgtc
      241 agcccctcgc ccgcaccgac ctcgatcccg atccgatcaa gcagttcgga gcgtggttcg
      301 aagctgccgc tgagatcgtg gccctgccgg aggccatgac cctggccacg gttgacgagg
      361 cggggcgacc tgacgcacgc atggttctgc tcaagggcca cggagccgac ggattccggt
      421 tcttcaccaa ctacgaggga gtcaaggcag cccagctcga tgccagcggg gaggccgccc
      481 tggtcttctt ctgggcgccg caggaccggc aggtccgggt gcggggcccg gtgcgacggc
      541 tggacgcgga tgcctctgac cggtatttcg cgagtcggga ccgggcgagc caggtcggcg
      601 cctgggcttc cccccagagc cagccgattc ccgaccagag ggaggcgctc gaagagctgg
      661 ccgaagcggc ggccgatcgt ttcgcggaag gcgaagtacc gcgaccccag cattggggag
      721 gtttcatcct ggagccccgc tcgatcgagt tctggcaggg tcgcggggcc cggctccacg
      781 accgttttcg ctacgaccgc caggattcgg gagagggctg gaagatcacc cgcctggctc
      841 cctgagcgaa aagggtccgg ttctttcgcg gtcaccggct gcatgacatc tcgcaggggg
      901 ctggacaacc tgccccgaac ctgtttagcc tgcaagcctc aggggtgatg tccgtttttc
      961 cgttaaaagt ccggttggcg tttttcgccg ccctcgccgc agtccttctg gttccatcgg
     1021 cggctgaagc ccagacctgg tccggcggag gcatcacgct gcccggtccg accaccaacc
     1081 cgaacatcgg caagatcctg ccgctggccc ggcgtgagtt ttcccgcaac gtggtcgaac
     1141 gccgcggcaa caacgttccc cgctaccgaa acggcaaggg caggattgcg ccgtacagca
     1201 tctcggatca gtggtgcgcc gccttctcga cctggatctg gaaccacgcc ggcttcaagt
     1261 cctacctcgg gacgagcatc ctctggccgt catatgacgg caccatggtc ggggtccagg
     1321 tccgggacct gaccaactgg gcgaccagga acggctactt ctcctaccgg gcgaaaccgg
     1381 gctacctcgt cgcttacgga accacccaca tcggcatcgt cgagaagatc gaccggacca
     1441 ccggtcgcgc ggtcggctcg atcgagggca acatcggcaa ccgggtaaac cgggtgaccg
     1501 tgccgatgga ccgggtcacc ggatacatca gcccggtgcg gctgaccccg atccagctcg
     1561 acaagagtgc cgcctacgct gacatggcgg tcccaccggc cgcgctcgag cgggccgaag
     1621 cttccgggtt cagccagccg taaggtgatc catcaatgac acgcagccgg agatctccgg
     1681 ttgcgcgccg ttgctgccgg cgggcttgcc gatcaggcga ccatcccagc agcccgccca
     1741 gtcgaccttg acctcgtaac gggccgcgct gccgtcgacc tctttggtgc agacccagcc
     1801 gcttccgccg ggcctgcagt cgtgggcgga tgcggccccg accgacttgg cgatcgaggc
     1861 gtagttggtg ccgaggtacg gagtcggcgc gaagaggacg accagcgaga gggccagcag
     1921 gaccagggcg accgcgaaac caccgaccag gatgatcctg gtccggtgac cgcgataagg
     1981 gttgttccgg cgctgcccga accggcgatc gacgcgttcg gcattccgct cgctccgggt
     2041 cttgtattcg ggcagcccgc tgaaatcttc gctcacacca tccatcgcgc gagcagagta
     2101 gaccgccact ttcagccagg tccgaaaatc cctgctaatg aacggaaagc tgccgtcagc
     2161 ggaagccggc cagcatgaag ttgtctcttt ccgaggcgaa cttcatcgac ccggcgatga
     2221 ccgttctcct gccgtcgaag tagacgccgt tgccgatcgc gttgggccgg ttgccgaggc
     2281 tcaggaaagc ccgcttggcg aaggcccggt cgacctttcc gttcggctcg accctcttga
     2341 cgagcaacgg gcgggagcag ccccagttct cggcgcaggc cgggcggctg atcccggtca
     2401 cgaccacgct tccgtcgcca cgaaccccga tgccatacgg acgggccggg aagggcttcc
     2461 ggtagtacgg cttctggctg gtgttcgcct ggggcaggat gaccacgcca cccttgccgt
     2521 acctgcgcac cggaaaaccc cgtgagttga gtttggccac gaagggcttc ttcgaataga
     2581 ggccggtgag gtagatgctg ccgtcgcggc cgaggaccac gccgttggtc ttggtcaacc
     2641 cggggagata catcggcttc tgaccgtcct tttcctggga tacagaatcg gtgccgtagt
     2701 gaagcttgcc gccgatgccg aagctcttgt cggggaagcc gttctttgtg tagcgcacga
     2761 tgtaccgggc atcaccgctg gaggcgatga acagggtccg tcccctgcgg tcggttgcga
     2821 ggctggatga atagacccag tagaaatcga ggtcggccat ctccggtcgc tgggcgaccc
     2881 cgtctacccc gtagctgcgg tcgacttgac cgtcaggcaa caccctggtc agatcaacgc
     2941 cgcggcggtc ttcgacgatg tccatcgccg agatcacggc accggtcccg tcaaacgaaa
     3001 tgtcctggca gcgaggcgag aagtccaggg tcaggcgccc gtcgtcacca aagctcttga
     3061 cgagatgccc gtaacggctg atccgccaga ccaccgagtc ccggccgtcc ttggcgcaaa
     3121 tcatcgcccc gcccgaaggc agcagggtca tcttcggttt cggatcgaag cgggccgcag
     3181 gttgggtcag caccaggcgt ccgcggtcac cgaacttcgt ttccagcctg ccgtttcgcc
     3241 cgaacgaggc gaccgcgatg ccgtagccgt atctcgaacc ctgggcgatg gttccgaggc
     3301 tcaaataggt gtgatcccgg cgctgggcgg agtcggtcag gttgctgtcc ggcaggtagc
     3361 cggtcc
//
LOCUS       DAOQFI010000120         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13501_length_3362_cov_2.912307, whole genome shotgun sequence.
ACCESSION   DAOQFI010000120 DAOQFI010000000
VERSION     DAOQFI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13501_length_3362_cov_2.912307"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLE93_03040"
     CDS             complement(<1..334)
                     /locus_tag="PLE93_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNH86055.1"
                     /translation="MNRVPVLVVGGGGAGLTASMLLANLDIEHLLINSRPGTSDLPKA
                     HVLNLRAMEILDDVGAADRISEKSTPPEAMAATAFYAGFGGDDPDARRRIAKLQSWGA
                     GGTDGDWRE"
     gene            396..1064
                     /locus_tag="PLE93_03045"
     CDS             396..1064
                     /locus_tag="PLE93_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH86056.1"
                     /translation="MTVHTSSIREKNDTKPPGRVERRKAKTRASLIEAARRLFASKNI
                     ESTSVAEIAEEADIAVGSFYNYFETKDELLAALLESTLVEQLARIRARQSQTDDPAEK
                     VAIGHRHLVDLATREPDLAWLIVRFEVPQRIASLGLAEAARADMMEGIATGRFDVRNP
                     ELSLRASGGALLGVIHTILLGELPGDSGGDLAAGVLMGLGMKPAEAREVAARPLPPES
                     LEAE"
     gene            1065..2063
                     /locus_tag="PLE93_03050"
     CDS             1065..2063
                     /locus_tag="PLE93_03050"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH86057.1"
                     /translation="MTTGSIDVHSHYIAPAYRSALESVGKDRIGGIPVPPWTPELAIG
                     FMDAHGIARQVLSVSDPGVDFLAGEAAAKLARECNDYLAGVVASHPDRFSGLAVVSGN
                     DPEAALSEIARCLERPGFAGLGLLSSYDGSYLGDPAFDPLLTELDQRRAWVMVHPTAV
                     PTTDKPDLTIPDFMAEYPFDTTRAFLNLLVHNAFARFPGIRWHFAHGGGTIPMLAARM
                     DVAAAHGKVLGPLLGLPEQSADLGPESAREALAGCWYDTALIASEGGLQAVSAMTGTD
                     HILFGCDWPFAGLAYPPEGDPQPAIGTLFGPDDQEAIYRANAEKLLAGLPQQTESA"
     gene            complement(2156..2761)
                     /locus_tag="PLE93_03055"
     CDS             complement(2156..2761)
                     /locus_tag="PLE93_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86058.1"
                     /translation="MSNLVTRIKQFRPATLIAMLALFVAIGGTATAASGLINGKKIKN
                     NTVTGKKLKNRTITKNKLAPATVKALKGQQGPKGDTGQPGKDGVVSPTYQEFGSTNIV
                     DGNELAIGTVNVPAGKYMVTATANTFALGSGRTECFVTTNNGGGSSETSSWNSTAANV
                     RNALPVVYVTDTDTVTSITLGCGIDDTNGSVGGSMIVTPVQ"
     gene            complement(2886..3284)
                     /locus_tag="PLE93_03060"
     CDS             complement(2886..3284)
                     /locus_tag="PLE93_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNH86059.1"
                     /translation="MGERWRIGSGAVWEERFGYSRAVKAGPLIFVSGTTAADPTGGPP
                     IGGDDIAAQARECFRRIEVALGELGSGLGEVVRTRMFVTDIGLSEAVGLVHGEIFGRQ
                     APAATMVEVSALISPDLLVEIEVDAVSSSD"
BASE COUNT          548 a         1129 c         1055 g          630 t
ORIGIN      
        1 cctcgcgcca gtccccgtcg gtccctcccg ctccccagct ctggagcttc gcgatgcggc
       61 gtcgggcgtc cgggtcgtcc ccgccgaagc cggcgtagaa ggcggtcgcc gccattgcct
      121 ccggcggcgt gctcttctcg ctgattcggt cggcggcgcc gacgtcatcg agaatctcca
      181 tcgccctcag gttgaggaca tgcgcttttg gcaggtccga agtcccggga cgggagttga
      241 tcagcagatg ctctatgtcg aggttcgcaa ggagcatcga ggccgtcaat ccggccccgc
      301 cgcctcccac gaccagtacg ggtactcggt tcatgtcgac gaccgtagca caaacatgaa
      361 cacttattca agtttgacgc tatactcatg ttgtcatgac cgtccatact tcatcgatcc
      421 gggaaaagaa cgacaccaaa cccccagggc gggtcgagcg acgcaaggcg aagacacgtg
      481 cttccctgat tgaggccgcc aggcgactgt tcgcctcgaa gaacatcgag tcgaccagcg
      541 tcgccgagat agccgaagaa gcggacatcg ccgtcggctc cttctacaac tacttcgaga
      601 ccaaggacga gctgctcgcc gctctgctcg aaagcactct ggtcgagcaa ctcgcccgga
      661 tccgggcccg gcaatcgcag accgacgatc cggccgagaa ggttgcgatc gggcaccgtc
      721 acctggttga cctggcgacc agggagcccg accttgcctg gctgatcgtc cgcttcgagg
      781 taccccagcg aattgccagt ctcggcctcg ccgaggccgc ccgagcggac atgatggaag
      841 gcatcgccac cggccgcttt gacgtgagga acccggagct gtcgcttcgc gcctccggcg
      901 gagccctgct tggcgtgatc cacaccatcc tgctcggaga gttgcccggc gactccggcg
      961 gggatctcgc tgccggcgta ctgatgggcc tcggcatgaa accggccgag gcaagggagg
     1021 tcgcagcccg gccccttccc cccgaaagcc tggaggcaga atagatgaca accggatcga
     1081 tcgacgttca ttcgcactac atcgctcccg cgtaccggtc ggcgctcgag tcggtcggca
     1141 aggaccggat cggcggcatt ccggtgccac cctggacccc cgaacttgcg atcggcttca
     1201 tggacgccca cggaatcgcg cgacaggtgc tttcggtctc cgatcccggc gtcgacttcc
     1261 tcgccggcga ggcggcggcg aagctggccc gcgaatgcaa cgactacctc gccggcgtgg
     1321 tcgcctccca tcccgatcgc ttcagcggcc tggcagtcgt atccggaaat gatccggaag
     1381 cggcgctttc cgaaatcgcc cggtgcctcg agagacccgg atttgcaggc ctcgggctgc
     1441 tctcgagcta cgacggcagt tatctcggtg atcccgcctt tgacccgctc ctgaccgaac
     1501 tggaccagcg gcgtgcctgg gtcatggtcc acccgaccgc tgtccccacc acggacaaac
     1561 ccgacctgac catccccgac ttcatggccg agtacccgtt cgacaccacc cgggccttcc
     1621 tcaaccttct ggtccacaac gcgtttgccc gctttcccgg cattcgctgg cactttgccc
     1681 atggcggagg caccatcccg atgctcgccg cccgcatgga cgtggccgcc gcccatggca
     1741 aggtgctcgg cccgctcctc gggctaccgg agcagtcggc cgatctcggt cccgagagcg
     1801 cccgggaggc gctcgccgga tgctggtatg acacggcgtt gatcgcttcg gaaggcggac
     1861 ttcaggcggt tagcgcgatg accggcaccg atcacattct cttcggctgc gactggccct
     1921 ttgccggtct cgcctatcca cccgagggtg acccccagcc ggcgatcggc accctgttcg
     1981 gcccggacga tcaagaggcg atctaccggg ccaatgcgga aaagctgctg gccggccttc
     2041 cgcagcagac cgaatcagcc tagggcgcga gggccagggc gacgacgatg cacgaacgcc
     2101 gcccccgttc ccgggggcgg cgcgtgtagc tctatggctt cctgccggag ggtcgttact
     2161 ggaccggggt gacgatcatc gaaccgccga ccgagccgtt ggtgtcatcg atgccgcagc
     2221 caagggtgat cgaggtgacc gtgtcggtgt cggtgacgta aacgaccggc agggcgttgc
     2281 ggacgttcgc ggcagtcgag ttccagctcg aggtttcgct gctgccgccg ccgttgttgg
     2341 tcgtgacgaa gcattcggtc cggccgctgc cgagggcgaa ggtattcgcc gttgccgtga
     2401 ccatgtactt gccggccggg acgttgacgg tcccgatcgc gagttcgttt ccgtcgacga
     2461 tgttggtgct gccgaactcc tggtaggtcg ggctgacgac gccgtccttg ccgggctggc
     2521 cggtatcgcc cttcgggccc tgctgcccct tgagggcctt gaccgtggcc ggagcgagct
     2581 tgttcttggt gatcgtcctg ttcttcagtt tcttgccggt gaccgtgttg ttcttgatct
     2641 tcttgccgtt gatcaggccg gaagccgcgg ttgcggtgcc gccgatggcg acgaagaggg
     2701 ccagcatggc gatcagggtg gccgggcgaa actgcttgat gcgggtgacg aggttgctca
     2761 ttgttgcctt tcgttgtgct tcggttgatt ggcgcttctg attggtcaac tggcgagcag
     2821 ccaattactt acgcggattt cgaaaatctt ttccggaacc ccgatatttc gcttagcaga
     2881 gcggttcagt ccgaggaaga aactgcgtca acctcgatct ccacgagcag gtcggggctg
     2941 atcagggccg agacctcgac catggtcgcc gccggtgcct gcctgccgaa gatctctccg
     3001 tggaccagcc cgaccgcttc gctcaggccg atgtcagtca caaacatgcg ggttcgaacg
     3061 acttcgccga ggcccgagcc gagctccccg agcgcgactt cgatccggcg aaagcactcc
     3121 cgggcctggg cggcgatgtc atcgcctccg atcgggggtc ctcccgtcgg atccgccgcg
     3181 gtggttcccg agacgaagat caggggcccg gccttgacgg cccgcgaata gccgaatctt
     3241 tcttcccaga cggcgccgga gccgattctc catctctccc ccatgtccgg ttcagcgtat
     3301 ccgcgcggtg gccggattac cattgcgtca tgcccacctc cgaagagaac gacaaggccc
     3361 gg
//
LOCUS       DAOQFI010000121         3346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13615_length_3346_cov_2.759040, whole genome shotgun sequence.
ACCESSION   DAOQFI010000121 DAOQFI010000000
VERSION     DAOQFI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13615_length_3346_cov_2.759040"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLE93_03065"
     CDS             <1..812
                     /locus_tag="PLE93_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86060.1"
                     /translation="SIPATVAMVRADGFDMPIEIWELGRRYLDGRPFQQHGHADESTR
                     LITTAIGEGARYVVLQQYFDKPENEMYGLIDGDGNSRQARFAVRNTLSMLRDTRSAER
                     LRAGSGTDGYRFDRPGGWRRVLWATGDELKPGKALGIRSTTVAVTGNQGGTTHLPVSS
                     VTIGTSPQWFEPDMVEIWRQPARRKGFARVKARCPVASPTRYCQGWIKLWGGKGNRTL
                     VAAGKYRIGRGTTRVTGIRRKRTRAKARVIFATPFSCPGKLGNCRSWQNLP"
     gene            complement(827..1276)
                     /locus_tag="PLE93_03070"
     CDS             complement(827..1276)
                     /locus_tag="PLE93_03070"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNH86061.1"
                     /translation="MRILFVCLGNICRSPSAEAVMAGLIVREGIEGIEIDSAGTGDWH
                     VGQPADPRAIAAATDRGVEISGLARQVRPSDFEEFDLILAMDSSNHANLLAMDGADPE
                     KVRLFREMAGEGELDVPDPYYGGEDGFAEGLDILESGCAVILEEINA"
     gene            complement(1285..1782)
                     /locus_tag="PLE93_03075"
     CDS             complement(1285..1782)
                     /locus_tag="PLE93_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86062.1"
                     /translation="MTGKTKTTLAIIFGVVGILLGILGTITAFNAKNAVDDDKSQTSE
                     VAALVEQKFNEAQAKQDQAEANQRSEAEKLVAQLTQGKKNLLQKINTNHRQLKKVRRQ
                     VKNLSGQVSSLQSRDRELQNEIDQIENDQQADYNQLNGRINNLNRNVQQLQKQFARLN
                     GEING"
     gene            complement(1817..2044)
                     /gene="secG"
                     /locus_tag="PLE93_03080"
     CDS             complement(1817..2044)
                     /gene="secG"
                     /locus_tag="PLE93_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNH86063.1"
                     /translation="MYTIMAIIQVLLSVLLIFLVLLHSGKDAGLSGAFGIGGGGSSIG
                     GGSMVEQNLDRATVVTAILFTLNTILLIKLS"
     gene            complement(2057..2335)
                     /locus_tag="PLE93_03085"
     CDS             complement(2057..2335)
                     /locus_tag="PLE93_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86064.1"
                     /translation="MTVVWFIVLLVLVAVFVSMPFWRRGEASGTEDPEIAALEAARES
                     KYREIRDAEMDRESGKLSEEDFRELDAELRREAVGILDRLEALKGKES"
     gene            complement(2332..>3346)
                     /locus_tag="PLE93_03090"
     CDS             complement(2332..>3346)
                     /locus_tag="PLE93_03090"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNH86065.1"
                     /translation="PVAVVLVLFTGVGPMMSWRRISWEGAKRVFTVPLIAAAVAFVLL
                     LLFTDAAESIWALLIFTLAAFTVTALFQETWRAAAARKKSAGGTMLASIWSNATRNRR
                     RYGGYVVHLGLVVLLVGIAASSSFETKQDIELKPGQSAVVDDYTVTYLNPTLTRDRQA
                     LVFGAELQVDRGGKSFARLNPTRQYHQATDGSAPPGIASYFEGEATSEVGLKAGLRSD
                     FWTSVEPNTDEFQRRARLADGLFGAYMRKKVLPIVQADPAKAETVSRAAENLLSLTTD
                     KVIAGYNSDSIEVPVTIRVIVSPMVTWIWIGAVIAIIGALFAAWPSGLLTRKRGGKGR
                     KAA"
BASE COUNT          573 a         1097 c         1072 g          604 t
ORIGIN      
        1 ccagcattcc ggcgaccgtc gcgatggtca gggccgacgg tttcgacatg ccgatcgaga
       61 tctgggagct cggtcgcagg tacctggacg gacgaccgtt tcagcagcat ggtcacgccg
      121 acgagtcgac ccggctgatc accaccgcga tcggcgaagg cgcccgttac gtggtgctcc
      181 agcagtactt cgacaagccc gaaaacgaga tgtacggact gatcgacggt gatggcaact
      241 cgcgccaggc ccgtttcgcc gtccgcaaca cgctttcaat gctgagggac accagatccg
      301 ccgagcggct ccgggccggt tcaggcaccg atggttatcg gttcgaccgg cccggcggct
      361 ggcggcgggt tctctgggcg accggcgacg agttgaaacc gggcaaggcg ctcggcatcc
      421 gctcgaccac ggtcgccgtg accggcaacc agggcggcac cacccacctg ccggtctcct
      481 cggtcacgat cggaacctcg ccgcagtggt tcgaaccgga catggtcgag atctggcgcc
      541 agcccgccag gcgcaagggc ttcgcccggg tcaaggcccg ctgcccggtc gcgtcaccaa
      601 cccggtactg ccagggctgg atcaagctgt ggggaggcaa gggcaaccgg accctggtcg
      661 ccgccgggaa gtacaggatc ggacggggca cgacccgtgt caccgggatc cggcgcaagc
      721 ggacaagggc gaaggcgcgg gtcatcttcg ccacaccgtt cagctgcccc ggcaagctcg
      781 ggaactgccg cagctggcag aacctgccct gacccgcgca gcgcggtcag gcgttgatct
      841 cttcgagaat cacggcacag ccgctctcga ggatgtccag cccctctgcg aacccgtcct
      901 cccccccgta gtaggggtcg ggcacatcaa gctctccttc gcccgccatt tcccggaaga
      961 gcctcacctt ttcggggtcg gctccgtcca tcgcaagcag attcgcgtgg ttggagctgt
     1021 ccatcgcgag gatcaggtcg aactcctcga agtccgaggg ccggacctgg cgggcgaggc
     1081 cgctgatctc gaccccgcgg tcggtcgcgg ccgcgatcgc ccgcggatcg gccggctggc
     1141 cgacatgcca gtcgccggtg ccggccgaat cgatctcgat tccctcgatt ccttcgcgca
     1201 cgatcagccc ggccatcacc gcctcggccg agggcgaacg gcagatgttg ccgaggcata
     1261 cgaaaaggat ccgcaagtcc gggctcagcc gttgatctcg ccgttcagcc gggcgaactg
     1321 cttctgcagc tgctgaacgt tcctgttgag gttgttgatc cggccgttca gctggttgta
     1381 gtcggcctgt tgatcgtttt cgatctgatc gatctcgttc tgaagctcgc ggtcccggct
     1441 ctggagcgaa ctgacctgcc ccgagaggtt cttcacctgc cgacgaacct tctttagctg
     1501 cctgtggttt gtgttgatct tctggagcag gttcttcttc ccctgggtca gctgggcgac
     1561 cagcttctcg gcctctgaac gctggttggc ttcggcctgg tcctgcttgg cctgggcttc
     1621 gttgaacttc tgctcgacca gagccgcgac ttcgctggtc tggctcttgt catcgtcaac
     1681 cgcgttcttc gcgttgaaag cggtgatcgt gccgagaatc ccgagcagga ttccgactac
     1741 gccaaaaatg atcgccagtg tcgtcttggt cttgccagtc atggcgctga ccctagacca
     1801 acaacgggga tccggcttaa gaaagcttga tcaggaggat cgtgttcagg gtgaacagga
     1861 tcgcggtgac aaccgtcgcc cggtcgagat tctgctcgac catcgagccg ccgccgatcg
     1921 aactgccgcc gccaccgatg ccgaaagcgc cggagagacc cgcgtccttg cccgaatgca
     1981 ggagcaccag gaagatcagg agcacggaaa gcaggacctg gatgattgcc atgatcgtgt
     2041 acatcggcca gcgattctag ctttctttgc ccttgagcgc ctcgaggcgg tcgagaatgc
     2101 cgaccgcttc ccgtctcagt tcggcgtcga gttccctgaa atcctcctcc gagagcttgc
     2161 cggactcgcg gtccatctcg gcgtcgcgga tctcccggta ctttgactcc cgggccgctt
     2221 cgagagcggc aatttcggga tcctcggtgc cggaagcctc gccgcgacgc cagaacggca
     2281 tcgagacgaa gaccgcgacc aggaccagca ggacgatgaa ccagacgacg gtcacgcggc
     2341 tttccttccc ttcccgcccc gtttccgggt caggagtcca ctcggccagg ccgcgaaaag
     2401 cgcaccgatg atcgcgatca cggcgccgat ccagatccat gtgaccatcg ggctgacgat
     2461 cacccggatc gtgaccggaa cctcgatgct gtccgagttg tatccggcga tcaccttgtc
     2521 ggtggtcagg ctcagcaggt tctccgccgc acggctgacc gtttccgcct tggcaggatc
     2581 ggcctgaacg atcggcagga ccttcttgcg catgtaagcc ccgaagagtc cgtcggcaag
     2641 cctcgcccga cgctggaact cgtccgtgtt cggctcgacc gaggtccaga agtccgacct
     2701 gagcccggcc ttcaggccga cctcgctggt cgcttccccc tcgaagtagg aggcgatgcc
     2761 gggcggcgcg ctgccgtcgg tggcctggtg gtactgccgg gtcgggttca gccgggcgaa
     2821 ggacttgccc ccgcggtcaa cctggagctc ggctccgaag acgagggcct gcctgtcgcg
     2881 ggtcagggtg ggattcagat aggtgacggt gtaatcgtca accaccgcgg actggccggg
     2941 cttcagttcg atgtcctgtt tcgtttcgaa gctggatgag gcggcgatcc cgaccagcag
     3001 cacgaccagg ccaaggtgga ccacgtaccc gccgtagcgc cggcgattgc gagtcgcgtt
     3061 ggaccagatg gaagcgagca tcgtgccgcc ggcgctcttc ttcctggccg cggccgcgcg
     3121 ccaggtttcc tggaacagtg cggtgaccgt gaaggcagcg agggtgaaga tcagtagcgc
     3181 ccagatgctt tccgcggcat cggtgaaaag cagaagcagg acgaaggcga ccgccgcggc
     3241 aatcaggggg accgtgaaga cgcgcttcgc tccctcccag gagatccggc gccagctcat
     3301 catcggcccg actccggtga agaggacgag cacgaccgcg accggg
//
LOCUS       DAOQFI010000122         3333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13725_length_3333_cov_3.221171, whole genome shotgun sequence.
ACCESSION   DAOQFI010000122 DAOQFI010000000
VERSION     DAOQFI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13725_length_3333_cov_3.221171"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /gene="metK"
                     /locus_tag="PLE93_03095"
     CDS             complement(<1..988)
                     /gene="metK"
                     /locus_tag="PLE93_03095"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNH86066.1"
                     /translation="MTDLKLAEGEHLFTSESVTEGHPDKIADQISDTILDACLSQDPH
                     SRVACETLVNTGMAVISGELTTSAQIDITDLVRKTVCEIGYDKGEYGYDGNNISVLVS
                     MDKQSPDIAQGVDTGIETRTGGSSDQFDLDGAGDQGMMFGYACNETEALMPMPIQIAH
                     RLSERLSLVRKDGTLPYLRPDGKTQVTVRYADGKPIAVERVLISTQHAPDVDNENTIR
                     PDLWENVILPVLAEEYGDLLDENALYESLLINPTGIFVIGGPVGDAGLTGRKIIVDTY
                     GGAARHGGGAFSGKDPSKVDRSAAYAARYAAKNVVAAGLADRCEVQVAYAIGV"
     gene            1208..1690
                     /locus_tag="PLE93_03100"
     CDS             1208..1690
                     /locus_tag="PLE93_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH86067.1"
                     /translation="MAMVRWEPSRELEAFQTDMNRLFDSFFRSGSNSTVRRWAPATDL
                     TEDGNDLVLRLDLPGLSDEDVAVEVEGNVLTVSGERRDERETKEGSAYRLERSFGKFS
                     RSFTLPEGTDPDAIEGGIDKGVLELRIPKPTEKQPRRVELGASREEARAAIEGTSSET
                     "
     gene            complement(1750..3096)
                     /gene="der"
                     /locus_tag="PLE93_03105"
     CDS             complement(1750..3096)
                     /gene="der"
                     /locus_tag="PLE93_03105"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNH86068.1"
                     /translation="MEAPGSSSAPSGTVPTVAIVGFPNVGKSTLVNRLAGGREAVVHS
                     EAGVTRDRKRVRCEWNGREFDLLDTGGIDLDDASEMAADIQRQARLGMAEADVVVLVV
                     NGQAGLRTGDTDLARELRTSPVPVIVAVNKSDRPDDEFAAAEFHALGLGDPTAVSATH
                     GIGTGDLLDRITEALGDRPARAEESDAVRVAVIGRPNVGKSSLVNRILGDDRVIVSPV
                     AGTTRDTIDTEIEVDGRSVILVDTAGLRRKSKVAGSVDHYAQLRSERAIERADVAIIV
                     CDAFEGVRSEDLKVGEVAMRAGCATMIVMNKWDITETDIDDTRARVGRKLRLRPEVVT
                     CSAKSGRNVAGLISRAVALADRASQRIPTPQLNRFISEVQTTTPPPSRRGRRLKLLYG
                     AQTGVSPPHFSIQVNDRRLIVRDWAYHLENRLRDQWGLDGVPLVIDYIPRKGRGDD"
     gene            complement(3096..>3333)
                     /locus_tag="PLE93_03110"
     CDS             complement(3096..>3333)
                     /locus_tag="PLE93_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNH86069.1"
                     /translation="KVFLTASPEARAERRAAETGQPVAEVLAAQEERDRRDSEREHGA
                     LYAAEDAVTIDSSGMSADQVIAEIAALARDRGLA"
BASE COUNT          580 a         1049 c         1121 g          583 t
ORIGIN      
        1 cgacgccgat cgcgtaggcg acctggacct cgcagcggtc ggcgaggccg gcggcaacca
       61 cgttcttggc ggcgtaacgg gctgcgtagg cggccgaacg gtccaccttc gacggatcct
      121 tgcccgagaa ggcgccaccg ccgtgacggg cggcgccgcc ataggtgtcg acgatgatct
      181 tgcggccggt caggccggcg tcaccgaccg ggccgccgat cacgaagatc ccggtggggt
      241 tgatcagcag cgactcgtag agggcgttct cgtcgagcag gtcgccgtac tcctcggcga
      301 ggaccggcag gattacgttt tcccagaggt cgggacggat cgtgttctcg ttgtcgacgt
      361 ccggggcatg ctgggttgaa atcaggaccc gttcgacggc aatcggcttg ccatcggcgt
      421 aacgaacggt gacctgggtc ttgccgtcgg gccgcaggta cggaagcgtg ccgtccttgc
      481 ggaccagcga gagccgctcg gaaagccggt gggcaatctg gatcggcatc ggcatcaggg
      541 cttcggtttc gttgcaggcg taaccgaaca tcatgccctg gtcgccggcc ccgtcgaggt
      601 cgaactgatc cgaagaaccg ccggtacggg tttcgatccc cgtgtccacg ccctgggcga
      661 tgtccgggga ctgcttgtcc atcgagacca gcaccgagat gttgttgccg tcatagccgt
      721 actcgccctt gtcgtaaccg atctcgcaga ccgtcttgcg gaccaggtcg gtgatgtcga
      781 tctgggcgga ggtcgtcagc tcgccggaga tcacggccat gccggtgttg accagggtct
      841 cgcaggcaac ccgggaatgc ggatcctgac tgaggcatgc gtcgagaatc gtgtccgaga
      901 tctggtcggc gatcttgtcg ggatggcctt cggttacgga ttctgaggtg aagaggtgtt
      961 caccctcggc aagcttcaga tcggtcattc gggaagtttc tccttggggt ctggcacaga
     1021 agcctcgctc agcgagtgca tgtggttgcg aagcctagat gaaacctcga tccagaatgc
     1081 ccgcgctact tcgaccggaa aatgtgcgtc tctcagactt gacatgtcgg cactcagatt
     1141 ttatgatggc cctcgaattg gtcggggact attccgagtc ctcgaaagac cagaaaggag
     1201 aaagcgaatg gcaatggtca gatgggaacc atcccgggaa ctggaagctt ttcagaccga
     1261 tatgaaccgg ttattcgaca gcttctttcg ctcgggttcc aattcgaccg tgcggaggtg
     1321 ggcgcccgcc accgacctga ccgaggacgg aaacgatctg gttctacggc tcgatctgcc
     1381 aggactgtcc gacgaggacg tggcggtgga agtcgagggc aacgtgctca ccgtctcggg
     1441 ggaacgccgg gacgaacggg agaccaagga aggcagcgcc taccggctgg aacgctcgtt
     1501 cgggaagttc agtcgctcct tcaccctccc ggagggaacc gatcccgacg cgatcgaggg
     1561 cgggatcgac aaaggggtgc tggaactgcg tatccccaag ccgaccgaga agcagccccg
     1621 ccgagtcgaa ctgggcgctt ccagggaaga agcgcgggca gcgattgaag gcacctcgtc
     1681 tgaaacctga cccgaggtga agaacaggac cgaaaaaggg gtcacgccgg tgaccccttt
     1741 ttcgtgtctt cagtcgtccc cccggccttt tcgggggatg tagtcgatca ccagcgggac
     1801 tccgtcgagg ccccactggt cgcgaaggcg gttctcgagg tggtaggccc agtcgcgcac
     1861 gatcaatcgc cggtcgttga cctggatcga gaaatgcggc ggcgacacgc cggtctgggc
     1921 tccgtagagg agcttcaaac gccgcccgcg acgggaaggc ggcggggtcg tggtctggac
     1981 ttcggaaatg aagcggttga gttgcggggt cgggatccgc tgggaggccc ggtcggccag
     2041 cgcgaccgca cgggagatca ggccggcgac gttgcgtcct gacttggccg aacaggtcac
     2101 aacctcgggg cgcaggcgga gctttcgccc gacccgggcc cgggtgtcat cgatgtcggt
     2161 ctcggtgatg tcccatttgt tcatcacgat catcgtggcg cagccggccc gcatcgccac
     2221 ctcccccacc ttcaggtcct cggagcgcac gccttcgaag gcgtcacaga cgatgatcgc
     2281 cacatcggcc ctttcgatcg cccgctcgga gcgaagctgg gcgtagtgat cgaccgaacc
     2341 ggcgaccttc gacttgcgac gaagccccgc ggtgtcgacc agaatcacgc tgcggccgtc
     2401 aacctcgatc tcggtgtcga tcgtgtcccg ggtggtgccg gcaaccgggg aaacgatcac
     2461 ccggtcgtcg ccgaggatcc ggttgaccag cgaggacttg ccgacgttgg gccggccgat
     2521 caccgcaacc ctcaccgcgt cagattcctc cgcccgggcg ggacggtctc cgagagcctc
     2581 ggtgatccgg tcgagcagat caccggtacc gatcccgtgg gtggcagaga ccgcggtcgg
     2641 atcaccaagg ccgagcgcgt gaaattccgc cgcggcgaat tcgtcgtccg gccgatccga
     2701 cttgttgacc gccacgatca ccggtaccgg cgaggtgcgt agctcgcggg caaggtccgt
     2761 gtcaccggtg cgcagaccgg cctggccgtt gacgaccagc acgacgacat cggcctcggc
     2821 catgccgagc cgggcctggc gctgaatgtc cgccgccatc tccgacgcgt catccaggtc
     2881 aatgccaccc gtgtcgagca ggtcgaattc gcggccgttc cactcgcagc gaacccgctt
     2941 gcgatcccgg gtgaccccgg cttcagagtg aacgaccgcc tcgcggccgc cagccagccg
     3001 gtttacgagg gttgacttgc cgacattggg gaaaccgacg atggcgacgg tcggcaccgt
     3061 ccccgacggg gccgaggaac tgcctggcgc ttccatcagg ccaggccccg gtcgcgggca
     3121 agagcggcga tctcggcgat cacctggtcg gcggacatgc cgctgctgtc gatcgtcacc
     3181 gcgtcttcgg cggcatagag ggctccgtgc tcacgttccg agtcacgccg gtcgcgttcc
     3241 tcctgggcgg ccagcacctc ggccacaggc tggccggtct cggccgcccg gcgctcggcc
     3301 ctcgcttccg gggaggcggt gagaaagacc ttc
//
LOCUS       DAOQFI010000123         3332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13733_length_3332_cov_2.549588, whole genome shotgun sequence.
ACCESSION   DAOQFI010000123 DAOQFI010000000
VERSION     DAOQFI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13733_length_3332_cov_2.549588"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLE93_03115"
     CDS             <1..721
                     /locus_tag="PLE93_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNH86070.1"
                     /translation="LRPSQEVPLDQEIEVLEGQRYVSRGGVKLENGLDALGIDVTGKL
                     CLDVGASTGGFTDCLVQRGARHVIALDVAYGQIDASLRDRTEITVIERLNARDLLPAD
                     LEYRPELTVIDVSFISLTKVLPAVVNTVAEDSTILAMVKPQFELGRARVGRGGVVRNQ
                     ADRREAGEAVADFALELGLSLDGIAPTGLPGPKGNLETFVQLGHSKASGSGGESTAGE
                     GSSAGARGADSGAALCEGVEL"
     gene            718..1521
                     /locus_tag="PLE93_03120"
     CDS             718..1521
                     /locus_tag="PLE93_03120"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNH86071.1"
                     /translation="MSRPTALLFTHSHAAGMDRAVEVAHAAAERHGWELLASKDAEGS
                     EPSLFIVLGGDGTILHALRSSLDRRVPVFGINFGRVGFLAGVEADQIELGLDRAFAGE
                     YEILDLPGLAISANGETQIALNDASFSRRPRGGVAELSYRIAGEEVGHVRCDGLVAST
                     ATGSTGYNLANHGPILAWGVAGYVVSYIAPHTLTARALVVAPGDVLHVMNHRSRDGVD
                     IAVDGVQIGELKPSAEAEVSYLPGLARLAQLPGSNFYHRIREKFGHLAV"
     gene            1626..3305
                     /gene="recN"
                     /locus_tag="PLE93_03125"
     CDS             1626..3305
                     /gene="recN"
                     /locus_tag="PLE93_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNH86072.1"
                     /translation="MLRELRIENLLLIERAELRLGPGLNVLTGETGAGKTVLAHSLDL
                     LMGGKAKKGIVRSGAPEAWVEGVFDLPPEWHGDEELADLLDRIPAGSEEVVLGRRVSE
                     SGRTSAFVGGRSASAADLQLLATRLIAFYGQHEHRRLTIGSAQLAMVDRAAGLKQQER
                     LNEYGSLWRRRRELARELDELLGRDMARERDLDLLRFELDEIEAASLIEGEKEVLTEE
                     RDRLRHAEGLREAAAGAGGRIRGGEDGEGAVGLLAGATDALAALKGVDADLDSLAGRV
                     ESLALEMEDVGLELGRYLERVEADPERLAELEERLDLIDRLGRKHGGTIESVLAHGAW
                     CREEIERLEGGESKEAALRSELEAAKASLDQAAKGLGRARRKAAGELAAEVTADLEDL
                     AMPGAVLSIELPPVADGPGPSGGESAEFMLAPNPGLPAQPLRDTASGGELSRVMLALA
                     ASGAGDRRTLVFDEIDAGIGGNTAAAVGERLRQVADGRQVIAITHLPQVASRAAAHFT
                     VEKSTGAGSVCATVTALDEDAVVTEITRMLGAGEGDEAAARHARELVMANR"
BASE COUNT          561 a         1071 c         1207 g          493 t
ORIGIN      
        1 actcaggccg agccaggagg tccccctgga ccaggagatc gaggtcctcg aaggccagcg
       61 atacgtctcg cggggcgggg tcaagctcga gaacgggctc gacgcgctcg ggatcgacgt
      121 caccggaaag ctctgcctcg acgtcggcgc ctcgaccgga ggcttcaccg actgcctcgt
      181 ccagcgtggc gccaggcatg tgattgcgct cgacgtcgcc tacggtcaga tcgacgccag
      241 cctgcgcgac cggactgaaa tcacggtgat cgagaggttg aacgcccggg acctgctccc
      301 ggccgacctc gaataccgcc ccgagctgac cgtgatcgac gtctcgttca tctctctgac
      361 caaggtgttg ccggcggtcg taaacacggt ggctgaggat tcgacgatcc tggccatggt
      421 caaaccccag ttcgaactgg gccgggcgag ggttggccgg ggcggagtgg tgcgaaatca
      481 ggcggaccgg cgggaggcgg gggaggccgt cgccgacttc gcgctcgagc tcgggctgag
      541 cctggacggg atcgcgccga ccggattgcc cgggcccaag ggcaatctcg aaacgttcgt
      601 ccagctcggg cattcgaagg caagcgggtc cgggggcgaa tcgacggccg gggaaggatc
      661 atccgccggg gcccgagggg ctgacagcgg cgccgctctc tgcgaaggtg tcgaactgtg
      721 agtcgcccca ccgccctgct cttcacccac tcgcacgccg ccggcatgga ccgggcggtc
      781 gaggttgccc acgccgccgc cgaacggcac ggctgggaac tcctggcgag caaggacgcc
      841 gagggatctg agccttccct gttcatcgtg cttggtggcg acggaaccat ccttcacgcc
      901 ctgcgttcat ctctcgaccg tcgggttcct gtcttcggga tcaacttcgg ccgggtcgga
      961 ttcctcgccg gggtcgaggc cgaccagatc gaactcggac tcgaccgggc cttcgccggc
     1021 gaatacgaga tcctcgacct gccgggactg gcgatatcgg ccaacggcga aacgcagatc
     1081 gcgctgaacg acgcgagctt cagccggcgg ccgcgcggcg gagtggccga actcagttac
     1141 cggatcgccg gggaagaggt ggggcacgtc cgctgcgacg gcctggtcgc ctcgaccgcg
     1201 accggctcca ccggatacaa cctcgccaac cacgggccga tcctggcctg gggcgtggcc
     1261 ggttacgtgg tgagctacat cgccccgcac accctgaccg cccgggcgct tgtcgtggcg
     1321 cccggcgacg tgctccatgt gatgaaccac cgcagccggg acggcgtcga cattgcggtc
     1381 gacggggtcc agatcggcga gctcaaaccc tcggctgaag ccgaggtcag ttacctgccc
     1441 ggcctcgccc gtctggccca gctgccggga tcgaacttct accaccggat cagggagaag
     1501 ttcggccacc tcgctgttta gcgcgcgctc tgcttaacag gggtcgggcg aggcggagcg
     1561 aagctcgccg acttgccagt taggcgaacg tttgttcgtc cgatggtccc gttagaacat
     1621 cagacatgct gcgcgaacta cggatcgaga atctgctgct gatcgagcgg gccgaactca
     1681 ggctcggtcc cgggctgaac gtgctgaccg gcgagaccgg cgccggcaag accgttttgg
     1741 cccattccct ggacttgctc atgggcggca aggcgaagaa ggggatcgtc cgcagcggcg
     1801 ccccggaggc ctgggtcgag ggcgtcttcg acctgccgcc ggaatggcac ggggatgagg
     1861 aactggccga cctgcttgac cgcatcccgg ccggcagcga agaagtggtg ctgggccggc
     1921 gggtttcgga gtccggccgg acctcggcct tcgtcggcgg ccgcagcgct tccgcggccg
     1981 acctgcagtt gctggcgacc aggttgatcg ccttctacgg gcagcacgaa caccggcgcc
     2041 tgaccatcgg gtccgcgcag ctcgcgatgg ttgaccgggc ggccggcctg aaacagcagg
     2101 agcgcctgaa cgaatacggg tcgctctggc gaaggcgtcg tgaacttgcc cgcgagctcg
     2161 acgaactgct cggccgggac atggcccggg aacgggacct cgacctgctc cgtttcgaac
     2221 tggatgaaat cgaggccgcc agcctgatcg aaggcgagaa ggaagtgctg accgaggaac
     2281 gggaccggct gcgccacgcg gaagggctgc gggaagccgc ggcgggcgcc ggtggccgaa
     2341 ttcgaggcgg cgaggacggt gagggggcgg tcggtctgct ggccggagcg accgacgccc
     2401 tggcggcact gaaaggtgtc gatgccgacc ttgactccct ggccggaagg gtcgagagcc
     2461 tcgctctgga gatggaggac gtcggcctcg aactgggccg gtacctcgag cgggtggagg
     2521 ccgatcccga acggctggcc gagcttgagg agcgactcga cctgatcgac cggctggggc
     2581 gaaagcacgg cggcacgatc gaatccgtcc tcgcccatgg cgcctggtgc cgcgaggaaa
     2641 tcgaacgcct cgaaggaggc gaatcgaagg aggccgcact gcggtccgag ctggaagcgg
     2701 caaaagcaag cctcgatcag gcagcgaagg gtctcggccg ggcccgcagg aaggccgccg
     2761 gcgaactggc ggccgaggtt acggcagatc tcgaggacct ggccatgccc ggggcggtgc
     2821 tttcgatcga actgccgccg gtggccgacg ggcccggtcc ttcgggcggg gaatcggccg
     2881 agttcatgct ggctccgaat cccgggcttc cggcccaacc gctccgcgac accgcatccg
     2941 gaggcgaact ttccagggtc atgctggccc tggcggcgag cggggcgggt gaccggagga
     3001 cgctcgtatt cgacgagatc gatgccggaa tcggcggcaa cacggcggcg gcggtcgggg
     3061 aacggctgcg acaggtggcg gatggccgtc aggtgatcgc gatcacccac ctgccgcagg
     3121 tggcaagccg ggccgcggct catttcacgg tcgaaaagtc gaccggcgcg ggttcggtct
     3181 gtgcgaccgt gacggcgctc gacgaggatg ccgtcgtcac cgagatcacg cggatgctcg
     3241 gagcgggcga aggcgacgaa gccgcggccc gacacgcacg cgaactggtg atggcgaacc
     3301 ggtaaccagc acttccctta cccttggggg gt
//
LOCUS       DAOQFI010000124         3329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13748_length_3329_cov_3.338424, whole genome shotgun sequence.
ACCESSION   DAOQFI010000124 DAOQFI010000000
VERSION     DAOQFI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13748_length_3329_cov_3.338424"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PLE93_03130"
     CDS             complement(<1..1320)
                     /locus_tag="PLE93_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86073.1"
                     /translation="MSAALAFGASGAQAASINVNSAFPYITGNDGWGLSGDASANFLV
                     YEPTVNVYAYKLPYSFGDPIVSNGTGNVEAFTGDWSLTMHNLGTTHILPEGKYRLDMI
                     QDPLLPYKTGGMASIGADAVIYVDRTGPQTSINSVVPNPTASTNANFTFSANDFNAAF
                     SETGSGVASFQCSKDGGAWFTCGNDDAFASIGEITYVAPEGIRNFRVRAIDRLGNIGD
                     PASFDWLVDLTDPEIHLPAISQDANQNVVPAYVLGENPPASGTCSDPLAGSPAGASGI
                     RSCVVDPVSTSQLGNFSRAASAEDKVGHTASTHGAYRVVPPRYRDLVAQDDPIAWYRL
                     DDPLGSSTLIDSSGHNHNGDAKNGLVLDRAPALACHQVVRPNGCEVAHDIRGRAAYFD
                     GKGAQGRVDGIVAPAGGFTLEAWVRPLDGGKMMIAQHGGSGALWIDGG"
     gene            complement(1401..1856)
                     /locus_tag="PLE93_03135"
     CDS             complement(1401..1856)
                     /locus_tag="PLE93_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HNH86074.1"
                     /translation="MSERTWRVYLAGEIHSDWRDRVEAACEGLPVVFTAPVTDHPASD
                     AAGTSTLGDEEKKFWADHKGAGVNSIRTQTFIQEADVVVVRFGDKYRQWNAAFDAGFA
                     SALGKPVITQHDEELDHALKEVDAAARATARTPEQVAAVLRYVITAELA"
     gene            complement(1892..2156)
                     /gene="ffs"
                     /locus_tag="PLE93_03140"
     ncRNA           complement(1892..2156)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLE93_03140"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(2189..3055)
                     /locus_tag="PLE93_03145"
     CDS             complement(2189..3055)
                     /locus_tag="PLE93_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNH86075.1"
                     /translation="MRSRDRRRSLAVRRTVALLVVLIALGIVAWAALNVSRLWKTDTH
                     GAELTETSIESKAVPGEHPVSVVVPDGADDGERGMLVFLHGRSGDDGSYTDDEEFFEA
                     LAGQGGDAPIVVFPDGGESSYWHDRDTGNWGSYVTDEVIPQMAGKFGADPDRVAIGGI
                     SMGGFGALDLAIQNPGMFCAVGGHSPALFLSGGESAPGAFDDAEDFDDNDVLATAAAD
                     PDAFSSQPLFLDAGKSDPFVPGVTELANSLEAAGVDATVKLNWPGGHDEDYWNAHWDE
                     YLAFYADALADC"
     gene            complement(3083..>3329)
                     /locus_tag="PLE93_03150"
     CDS             complement(3083..>3329)
                     /locus_tag="PLE93_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86076.1"
                     /translation="SVLFDTPTGGDGSVFRTISIGTVDQAGTVTGILGCSVNGGGKIC
                     SNSNEVSVPSGSLLVIRSAVSGTPPATMVHVGYTLGP"
BASE COUNT          616 a         1069 c         1042 g          602 t
ORIGIN      
        1 gcccccgtca atccagagcg cgccggaacc gccgtgctgg gcgatcatca tcttgccgcc
       61 gtccagcggc ctgacccagg cctcaagggt gaacccgcca gcaggggcga cgattccgtc
      121 cacccttccc tgggccccct ttccgtcgaa gtaggcagcc cggccacgaa tgtcatgagc
      181 aacctcacat ccgttgggtc ggacgacctg atgacaggcg agggctggcg cacgatcgag
      241 cacgagtccg tttttcgcgt caccgttgtg gttgtgaccg gaactgtcga taagggtcga
      301 ggacccgagc ggatcatcga gccggtacca ggcgatcggg tcatcctggg cgaccagatc
      361 tctgtacctc ggcggcacga ctctgtaagc gccgtgagtg ctcgcggtat gaccaacctt
      421 gtcctctgcc gacgcagcac gggaaaagtt accgagctgt gaggtactga ccgggtccac
      481 tacgcagcta cgaatgcccg aagcccccgc gggcgatcca gcgagcgggt cggagcaggt
      541 tcccgaggct ggcgggtttt cacccagaac ataggccggt acgacgttct ggtttgcgtc
      601 ttgggagatc gcaggcaggt ggatctcggg gtcggtcagg tcaacaagcc agtcaaagct
      661 cgcagggtcc ccgatgttgc ccagccggtc gattgcccga acccggaaat tccggatgcc
      721 ctcgggagcc acgtaggtga tttcgcctat tgaagcgaaa gcatcgtcgt tgccgcaagt
      781 gaaccaggcc ccgccgtcct tgctgcactg gaagctggca acgccggaac cagtctcgct
      841 gaaggctgcg ttgaagtcgt tagcgctaaa ggtgaagttc gcgttggtcg aggcggtcgg
      901 gttgggcacc acgctgttga tcgatgtctg aggacccgtc cggtccacat agatcaccgc
      961 gtccgccccg attgaagcca taccaccggt cttgtaggga agtagcggat cctgaatcat
     1021 gtcgaggcgg tatttgcctt cgggcagaat gtgggtggtc ccgaggttgt gcatggtcag
     1081 cgaccagtcg ccggtgaacg cctcgacatt gcccgtgccg ttcgagacga tcggatctcc
     1141 aaagctgtag ggcaatttgt aggcgtacac gttcacggtg ggctcgtaaa cgagaaaatt
     1201 ggcactggca tcgccgctca ggccccaccc gtcgttgcct gtgatgtaag ggaaagcgga
     1261 attgacgttt atcgacgctg cctgagcgcc gctggcacca aaagccaagg ccgccgacat
     1321 ggccagaaag atggccgcca gcctcacaat ttgcctagcc cccgagatca tctcgcggaa
     1381 gcatagcgaa cagtgtccgg ttacgccagt tcggcggtga tcacgtaccg gagaacggcc
     1441 gcgacctgct ccggggtgcg ggcggtggcg cgggcggcgg cgtccacttc cttgagggcg
     1501 tgatcgagtt cctcgtcgtg ctgggtgatt acgggctttc ccagcgctga agcgaatccg
     1561 gcgtcgaagg cagcgttcca ctggcggtac ttgtcgccga agcggacaac gacgacgtcg
     1621 gcctcctgaa tgaaggtctg ggtcctgatc gagttcaccc cggcaccctt gtgatcggcc
     1681 cagaacttct tctcctcgtc tccgagggtc gaggttccgg ccgcatccga agcagggtga
     1741 tcggtcaccg gggcggtgaa aacgaccggt agcccctcgc aagcggcctc gacccgatcc
     1801 cgccagtcgg agtgaatttc gccggcaagg tagacgcgcc acgttcgttc agacatgtgc
     1861 ccaacctacc cgtcaaggga aagaaaatgg accgtgcaac cgccttcgaa acgaagtcat
     1921 cgggcgtgtc cgtcgagttc ggctcgggtt aggctcaccc gcggcacccg tagtttccgc
     1981 ttaaggctgc ttccttccgg acctgacctg attcacgggc ttgcgtcgcg cgggacctga
     2041 ccgtcaacac ctccctcggt gtactgaccc cgatggccag accctcagaa cggcattcgg
     2101 ccttgctaaa gcggattgca agtgacaggg caccgctgac tccccgccta gcacggtccg
     2161 gttggaatcc tagcgccccg cgcagggttc agcagtcagc gagggcgtcg gcgtagaaag
     2221 cgaggtactc gtcccagtgg gcgttccagt aatcctcatc gtggccgccg ggccagttca
     2281 gcttcacggt ggcgtcgacc ccggccgctt cgagcgagtt cgccagttcg gtgacgccgg
     2341 gtacgaaggg gtcgctcttg ccggcgtcga ggaagagggg ttgcgacgag aaagcatccg
     2401 ggtcggctgc ggcggtagcg agcacgtcgt tgtcgtcgaa gtcctcggcg tcatcgaagg
     2461 cgccgggagc cgactccccg ccgctcagaa atagggccgg cgagtgaccg ccgacagcgc
     2521 agaacatccc cgggttctgg atcgccaggt cgagcgcacc gaaaccgccc atcgagattc
     2581 caccgatcgc aacccggtcc gggtcggccc cgaacttccc tgccatctgc gggatcacct
     2641 cgtcagtgac gtagctgccc cagtttccgg tgtcccggtc atgccagtag ctggactcgc
     2701 ctccgtcggg aaagaccacg atcggtgcgt ccccgccctg gcctgccagc gcctcgaaga
     2761 actcctcgtc atccgtgtag ctcccgtcgt caccgctgcg accgtggagg aacacgagca
     2821 tcccccgctc gccgtcatcg gcaccatccg gcacgaccac cgaaaccggg tgttcgccag
     2881 gtaccgcctt cgactcgatc gaggtctcgg tcagctcggc cccgtgggtg tcggtcttcc
     2941 acaagcggct cacgttcaac gccgcccacg cgacgatgcc caaggcgatc aggacgacaa
     3001 gcagtgcgac cgttcggcgc acggccagcg atcgccttct gtctctgctt ctcacggcca
     3061 agggccgaac cctacgaccg attcaagggc cgagggtgta ccccacgtgg accattgtcg
     3121 ctggcggagt ccccgaaacg gcacttcgga tgacgagaag tgaaccggac ggaacgctca
     3181 cctcgttcga gttggaacaa attttcccgc ctccgttgac cgaacaaccg agaatccccg
     3241 ttaccgttcc cgcctgatcg accgtgccga tggaaatcgt gcggaagact gagccgtcgc
     3301 cgccggttgg agtgtcgaac agcaccgag
//
LOCUS       DAOQFI010000125         3328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13758_length_3328_cov_2.721662, whole genome shotgun sequence.
ACCESSION   DAOQFI010000125 DAOQFI010000000
VERSION     DAOQFI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13758_length_3328_cov_2.721662"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PLE93_03155"
     CDS             complement(<1..1194)
                     /locus_tag="PLE93_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH86077.1"
                     /translation="MSIDRKGASDFTREAHVHDIRCLDFEDNESFEEAKRGFIAPVPD
                     GQILKDDGDFVFDPHRLAFASGDPEQPDTVNPSLWRQARLYADGGLFEVCDRIYQVRN
                     LDISNITFIEGDTGLIVVDPLVSAEVAKAALDLYFEHRGEKEVVAVIHSHSHVDHFGG
                     VRGVVDQADVDSGKVPIIAPEGFLEAAVSENVAAGNVMTRRAMYQFGILLPPDPQGTV
                     GSGLGIGTSLGTITLIPPTDLITETGETRKIDGLTFEFMLTPDTEAPAEMHWYIPELR
                     ALTAAENCTHTLHNTYPIRGAIVRDPQAWSMYLNDTIDRWADKSDVMYAMHHWPVWGK
                     DRVREMLEKGRDAYRYIHDETLRLANHGLTPREIAEQVEFPDSLAKHWSVRSYYGTVS
                     HNVKST"
     gene            complement(1191..2045)
                     /locus_tag="PLE93_03160"
     CDS             complement(1191..2045)
                     /locus_tag="PLE93_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH86078.1"
                     /translation="MEKAAVELLICPHCGGELRENGGSLICSRGHTMNIARQGYVSLL
                     GRDSGTHTADSAEMVAARERFLGAGHFQPLVDALVEEASGKPGPAGGESAVVDLGCGT
                     GYYLASVLDALPESIGVGVDNSKFAARRAAKVHPRASAVVADIWDGLPIKDQAAGLIL
                     NVFSPRNGEEIERILAPGGRLVVVTPQPGHLAGLIGRFGMISVDPDKEERLERTLGPL
                     AKAAGSRNLEWEMKLDRAAVRDLIQMGPSAGRMEEAEMESALQALEEVTAVEAAVTVT
                     TTSSEERQ"
     gene            2216..>3328
                     /locus_tag="PLE93_03165"
     CDS             2216..>3328
                     /locus_tag="PLE93_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH86079.1"
                     /translation="MPTFRRLLGFLRPHRRELWGSMVFAWLAMGMTVLIPALVGKATN
                     AIDSSDRQQLFWLVGAILLAAVLRLGLTVIRRIVAGRVSLAVEFDLRQEVYAHLMSLE
                     LGFFDRNQTGQLMSRATVDLQSIRFFLGYGLIFFSQSLLTVLLAGVVMFVINPWLALI
                     ALAPTPLIVFTALRYTRQSRPAMQEVAQRVAELTAQAEESVSGIRVVKAFAREDHQFE
                     RFQGAVDRLFDQSIRSTRIQAFFSPLIAALPQLGTALVLLVGGHEVLSGNLDLGQFTA
                     FYVYMLMLAGPLRTVGMALGMGQRAIASGNRLFEVLDREPELFSKPGAGPLPEGGGAV
                     RFDDVSLSYGPEAPDALQGIDLEVPAGTRIALVGPSG"
BASE COUNT          492 a         1134 c         1057 g          645 t
ORIGIN      
        1 ggtcgacttg acgttgtggc tgaccgtgcc gtaatagctg cggaccgacc agtgtttggc
       61 cagcgaatcc gggaactcga cctgctcggc gatctcacgg ggggtcagac cgtggttggc
      121 caagcgcagg gtttcatcgt ggatgtatcg atatgcgtcc cggccctttt cgagcatctc
      181 ccgaacgcgg tccttgcccc agaccggcca gtgatgcatc gcgtacatca catccgactt
      241 gtcggcccag cgatctatcg tgtcgttcag gtacatcgac caggcctgcg ggtcgcgcac
      301 gatcgccccg cggatcgggt aggtgttgtg cagggtgtgg gtgcagtttt cggccgcggt
      361 aagcgccctc agctccggga tgtaccaatg catttcggcc ggggcctcgg tgtccggggt
      421 cagcatgaac tcgaaggtca gcccgtcgat cttgcgggtc tcgccggtct cggtgatcag
      481 gtcggtcggg gggatcaggg tgatcgtgcc cagcgaagta ccgatgccga ggccgctgcc
      541 gaccgtgccc tgggggtccg gcggcagcag gatgccgaac tggtacatcg cccgccgggt
      601 cattacgttg ccggccgcca cgttctcgga gaccgccgct tcgaggaagc cctccggcgc
      661 gatgatcggg accttgcccg aatccacgtc cgcctggtcg accacccccc gcacgccgcc
      721 gaaatgatcg acgtggctgt gcgaatggat caccgcgacg acctccttct cgccccggtg
      781 ctcgaagtaa agatccagcg cagccttcgc cacttcggcc gaaaccagcg ggtcgaccac
      841 gatcagcccg gtgtcgcctt cgatgaaggt gatgttggag atgtccaggt tccgcacctg
      901 gtagatccgg tcgcagacct cgaagaggcc gccgtcggcg taaaggcggg cctggcgcca
      961 gaggctgggg ttcacggtgt ccggctgttc cgggtccccg gaggcgaagg cgagccggtg
     1021 cgggtcgaag acgaagtcac cgtcgtcttt caggatctgg ccgtccggga ccggggcgat
     1081 gaacccgcgt tttgcttcct cgaacgactc gttgtcttcg aagtccagac agcggatgtc
     1141 gtgaacgtgg gcctcccggg tgaagtcgga tgcgcccttg cgatcgatgc tcattgcctc
     1201 tcctcgctgc tcgttgtggt gacggtcacc gcggcctcca ccgcggtcac ctcctccagt
     1261 gcctggagcg ccgattccat ttcggcttcc tccattcttc cagcactggg ccccatctgg
     1321 atcagatccc tgactgccgc ccggtccagc ttcatttccc attcgagatt ccggctcccg
     1381 gctgccttgg cgagaggccc gagcgtccgc tcgagccgct cctccttgtc cgggtcgacc
     1441 gagatcattc cgaacctccc gatcagcccg gccaggtgtc cgggttgcgg tgtgaccacg
     1501 accagccggc cgccgggggc gagaatccgc tcgatctcct ccccgttgcg gggcgaaaag
     1561 acgttcagga tcaggcccgc cgcctggtct ttgatcggca ggccgtccca gatgtcggcc
     1621 acgacggcgc tggcccgcgg gtggactttc gcggcccggc gggcggcaaa cttggagttg
     1681 tcgaccccga ccccgatgct ttccggcaag gcgtccagaa ccgaggccag gtagtagccg
     1741 gtcccgcatc cgaggtcgac caccgccgac tcgcccccag ccggtccagg cttccctgat
     1801 gcttcctcga ccagggcatc gaccagcggc tggaagtggc cggcaccgag gaagcgttct
     1861 ctggcggcga ccatctcggc cgaatcggcg gtatgtgtgc cggagtcccg gcccagcagg
     1921 ctcacgtagc cctgccgggc gatgttcatc gtgtggccgc gggaacagat cagcgagccc
     1981 ccgttctcgc gaagctcgcc cccgcaatga gggcagatca gcagttcaac cgcggccttc
     2041 tccaccccgc gcactctatt tggctcgata gccccaccgc tcctgacgaa ctccgaaccg
     2101 ggatccaccc gacttcgcga atgcccactt tgtgccacat accggcacaa agtgggcatt
     2161 cgccgcccca atgtccctga ccaaccccat tacttcattt tgtatagtga aggggatgcc
     2221 caccttcagg cgacttctgg gattcctgag gccccatcgc cgcgagctct ggggttccat
     2281 ggtctttgcc tggctggcga tgggcatgac ggtgctgatc ccggccctgg tcggcaaggc
     2341 caccaacgcg atcgacagca gtgaccggca gcagctcttc tggctggtcg gggcgatcct
     2401 ccttgccgcg gtcctgcggc tcgggctcac ggtgatcaga cggatcgtcg ccggcagggt
     2461 ctcgctggcg gtcgagttcg atcttcgcca ggaggtctac gcccacctga tgagcctcga
     2521 gcttggtttc ttcgaccgca accagaccgg ccagctgatg agccgggcca cggtcgacct
     2581 gcaatcgatc cgcttcttcc tcggttacgg cctgatcttc ttcagccaga gcctgcttac
     2641 cgtgttgctg gcgggcgtgg tcatgttcgt gatcaatccc tggctcgcgc tgatcgccct
     2701 ggcccccact ccgctgatcg tgttcaccgc gctccgctac acccgtcagt cgcgcccggc
     2761 gatgcaggag gtggcccagc gggtcgccga gctcaccgcc caggccgagg agagcgtttc
     2821 cggaatccgc gtggtcaagg cgttcgctcg cgaggatcac cagttcgagc gcttccaggg
     2881 cgcggttgac cgcctgtttg accagtcgat tcgctcgacc cggatccagg ccttcttctc
     2941 gccgctgatc gccgccctgc cccagctcgg cactgccctg gttctgctgg tcggcggcca
     3001 cgaggtcctg agcggcaacc ttgacctcgg ccagttcacc gccttctacg tctacatgct
     3061 gatgctggcc ggcccgcttc gcacggtcgg catggcactg gggatgggcc agcgagcgat
     3121 cgcttccgga aaccgcctct tcgaggtgct ggaccgcgaa ccggagcttt tttcaaagcc
     3181 gggcgccggg cccttgcccg agggtggcgg tgcggtgcgg tttgacgatg tctcgctcag
     3241 ctacggcccg gaggcgccgg atgccctcca gggaatcgac cttgaggttc cggccgggac
     3301 ccggatcgcc ctggtcggcc cttccggt
//
LOCUS       DAOQFI010000126         3313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13870_length_3313_cov_2.889196, whole genome shotgun sequence.
ACCESSION   DAOQFI010000126 DAOQFI010000000
VERSION     DAOQFI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13870_length_3313_cov_2.889196"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(611..2275)
                     /locus_tag="PLE93_03170"
     CDS             complement(611..2275)
                     /locus_tag="PLE93_03170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNH86080.1"
                     /translation="MSQDRIKLLPLGGLGEIGKNMMVIEYRGSIVVVDTGLMFPTPEM
                     LGIDLVLPDFEYLRDRADDIEAIILTHGHEDHVGALPYALREIGFPKVIYGGMLTVGM
                     VRSKLDEHKLGSAPLTELPAGQKVSAGPFEIELVHLSHSIPDMRGVIIGTDLGKIFMT
                     GDYKFDQTPVDGRPADVARLAALGDEGLLLLCGDSTNADRPGVSPSESSVGPALYDTF
                     SRCKGRIIVTSFASNIHRVQQVIDAAERLDRKVALVGRSMRKNFNIASNLGMANAPEG
                     LLIQPREIEDFPDERVVVISTGSQGEPFSALRRMANNDHRDVELHSGDTVVFYATPVP
                     GNERAVNETIDRIYELGATVITPKDAPIHASGHGYREEIKMMLNLTRPKYVMPVHGDF
                     QRIRLHSELAESVGVPAENIFRNHNGQALLLGPDGAELTEETAGGVIYVDGVELSDPN
                     DATLRDRRTISADGIVIVVATIAEDDGEVVSDPEVIARGVPGLDEDAEFLDDLHDLIE
                     DSLDRAAEAKVRDHDLVQQDLHDAVAGFIHKRLKRRPMVVPVVVEV"
     gene            complement(2272..3141)
                     /gene="dapA"
                     /locus_tag="PLE93_03175"
     CDS             complement(2272..3141)
                     /gene="dapA"
                     /locus_tag="PLE93_03175"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNH86081.1"
                     /translation="MSEIRGVITALATAFRPDGELDIDSTRKLAAYLVENGSHGLVVA
                     GSTGESSTLEDDEKIDLTRAVVEEVGPEVTVIVGSGSNDTRHSVELTKMAAEAGADAA
                     LIVTPYYVRPNRAGIRRHFEAVAEGSPGFPIVAYNIPSRTGINMPPEFLAELAQIDEV
                     KAVKQANDDDLGPIEGLDVLAGNDGTFLPTLKFGGAGGILVASHLDNGRMRTIWDLWQ
                     EGRHDEAEALDNQLQPLYEAMGVVVNPIPVKAALAARGLLPPTMRLPLVEPTDQERDA
                     VEELLAEAGVPAA"
BASE COUNT          554 a         1061 c         1074 g          624 t
ORIGIN      
        1 cccagaaaca gcctccgaaa aacctcaagg aaaccgttaa cgaagcttta gggcctattt
       61 atgacctctc cgtatctttc ttcgcggacg agacacatga agccgccggg gatctccccc
      121 agactgcccc ggcggcttcg cttcttcaac acagaccgcc gagccacctc cctcccccag
      181 gcccggcggt cttgttgttt aacggcttga ctgcaagtcg ggcgggggtc cgacccgccc
      241 tcccagttca acccgtccga tcacgaaaaa aggatggatg atcggactct ggtcgaggtc
      301 cacgaacctt aagaagatcc ttccttcggg tttagggatt tcttactggg cgtccgtatc
      361 gttgcttttg gacgagacac aagaggttgt tgggcattct cccccagaca tccagcaacc
      421 tcgcttcttc agagcagacc gttgagccac tccctccccc aggcccagcg gtctctctgt
      481 tttaagcggc gggttcaggc cacgcttcat tcggctccat agagagcagg aaggtgccgc
      541 acgttcagcc ccgtgacggg gctttttctg cggcacctcc cggcgccgcg ctccaggcgc
      601 cgtgcgggcc tcagacttcg actactaccg gtaccaccat cgggcggcgc ttgaggcgct
      661 tgtggatgaa gccggcgacg gcgtcgtgga gatcctgctg caccaggtcg tggtccctta
      721 ccttcgcttc cgcagcgcgg tcgagtgaat cctcgatcag gtcgtggagg tcgtcgagga
      781 actccgcgtc ctcgtcgagc ccggggacgc cgcgggcgat cacttccggg tcggaaacga
      841 cctcgccgtc gtcctcggcg atcgtggcga ccacaatcac gatgccgtcg gcggagatcg
      901 tgcggcggtc gcgcagggtg gcgtcgttgg ggtcggaaag ttcgacgccg tcgacgtaga
      961 tcacgccgcc ggcggtttcc tcggtcagct ctgcgccgtc cgggccgagc agcaaggcct
     1021 ggccgttgtg gttgcggaag atgttctcgg ccggcacgcc aaccgattcg gccagctccg
     1081 agtggaggcg gattcgctgg aagtcaccgt ggaccggcat cacgtacttc ggccgggtca
     1141 ggttcagcat catcttgatc tcttcccggt atccgtggcc ggaagcgtgg atcggggcgt
     1201 ccttgggcgt gatcacggtc gctccgagtt cgtagatgcg gtcgatcgtc tcgttcacgg
     1261 cccgttcgtt gccgggcacc ggggtcgcgt agaagacgac cgtgtccccc gagtggagtt
     1321 ccacgtcccg gtggtcgttg ttggccatgc gccgcagggc cgagaagggc tcgccctggg
     1381 agccggtcga gatcaccacg acccgctcgt cgggaaaatc ctcgatctcc cgtggctgga
     1441 tcagcagccc ctcgggggcg ttcgccattc cgagattgga ggcgatgttg aagttcttgc
     1501 gcatcgaacg gccgaccagg gcgactttgc ggtcgagccg ctcggcggca tcaatcacct
     1561 gctgaacgcg gtggatgttg gacgcgaacg aggtgacgat gatccgcccc ttgcagcgcg
     1621 agaaggtgtc atagagggcc gggccgacac tggattcaga aggcgatacg ccgggccggt
     1681 cggcgttggt cgaatcgccg cagagcagga gcaggccttc gtctccaagc gccgcaaggc
     1741 gggccacgtc ggccgggcgg ccatcgaccg gggtctggtc gaacttgtag tcgccggtca
     1801 tgaagatctt gccgagatcg gtgccgatga tcaccccgcg catgtcgggg atcgaatgcg
     1861 agaggtgaac cagttcaatc tcgaagggcc cggcgctgac tttctggccg gccggcagtt
     1921 cggtcagcgg ggcgctgcca agcttgtgct cgtccagttt cgaacggacc attcccaccg
     1981 tgagcatgcc gccgtagatg accttgggaa agccgatctc ccgcagcgcg tacgggagcg
     2041 cgccgacatg gtcctcgtgg ccgtgagtga ggatgatcgc ctcgatgtca tcggcccggt
     2101 cgcgcaggta ttcgaaatcc ggcaggacga ggtcgatgcc gagcatctcc ggggtcggga
     2161 acatcagacc ggtgtcgacc accacgatcg agccgcggta ctcgatcacc atcatgttct
     2221 tgccgatttc ccccagtccg ccgaggggaa gcagcttgat ccggtcctgg ctcatgcggc
     2281 gggcacaccg gcctcggcga ggagctcttc gacggcgtca cgttcctgat cggtcggctc
     2341 gaccagcggc aggcgcatgg tcggaggaag caggccgcgg gcggcgagcg cggccttgac
     2401 cggaatcggg ttgacgacga cgcccatcgc ctcgtaaagg ggctgaagct ggttgtcgag
     2461 ggcctcggcc tcgtcgtgac ggccctcctg ccagagatcc cagatcgttc gcatgcggcc
     2521 gttgtcgagg tgtgaggcaa cgaggatgcc gcccgccccg ccaaacttca gtgtcggaag
     2581 aaaggtgccg tcgttgccgg ccagcacgtc gagcccctcg atcggcccga gatcgtcgtc
     2641 attggcctgt ttgaccgcct tgacttcgtc gatctgcgcc agttcggcaa ggaattcggg
     2701 aggcatgttg atcccggtgc gggaagggat gttgtaagcg acgatcggaa agccggggct
     2761 gccttcagcg acggcttcga agtgccggcg aattcctgcc cggttgggcc gcacgtagta
     2821 cggcgtcacg atcagggcgg cgtcggcgcc ggcttccgcc gccatcttgg tcagctcgac
     2881 cgagtgacgg gtgtcattcg aaccggaacc gacgatcacc gtcacttccg gcccgacctc
     2941 ttccactacc gccctggtga gatcgatctt ctcgtcgtct tccagggttg acgactcgcc
     3001 ggttgaaccg gccacgacca ggccgtggct gccgttctcg acgagatagg cggccagctt
     3061 ccgggtcgag tcgatatcga gctcgccgtc gggccggaaa gcggtggcca atgccgttat
     3121 tacacctcgg atctcactca caaaaggcac cctatccggc gtcgagaact cccggcgccc
     3181 ggcggccgat ccagggatgg gcccgctcga gctcgccggc gagctcgagc agcacgtgct
     3241 cgccccacat cggcgccacg aactggatcc cgatcggcag accttcctcg ctcatctcca
     3301 tcggcaccga aat
//
LOCUS       DAOQFI010000127         3311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13881_length_3311_cov_2.968980, whole genome shotgun sequence.
ACCESSION   DAOQFI010000127 DAOQFI010000000
VERSION     DAOQFI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13881_length_3311_cov_2.968980"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLE93_03180"
     CDS             <1..1035
                     /locus_tag="PLE93_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH86082.1"
                     /translation="KRAVVVGAGIAGLTAAWQLSRSGHEVTVLDRTGRTGGRILPAEF
                     SGRLVDTGPDALGPDPALDELLEQIGMAGQVEIPDPARAFRLTAAGELEPYGVGGGAM
                     FGLFGGIFSLPARLEELLREAGQKFRLGVTVDSVRQEDRSVLVEAEGSEEEFDLAVVA
                     TGARAAGRLLDGETGERLAAIRGVSVSLVHLAFPEKAIGREMNGTGYLADPAEGRLVT
                     GCSWSSAKWKRLAGDPAILRASIREAGQGEVIGLPDREVVEKVMAELVPVMEIEGDPV
                     ETMVTRHPEALVVRDEEHESAVAAARGLLAATPRIGLAGADLDGPGISRSIAGVRETV
                     RAIVEGTRND"
     gene            1028..1693
                     /locus_tag="PLE93_03185"
     CDS             1028..1693
                     /locus_tag="PLE93_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNH86083.1"
                     /translation="MTDTEETLHYCAYLSYSWDVEAGRGLAPAVEPLVAEIEAAGVEI
                     RGTYATTAFRAECDILFWLLADSTLKLDAAARAIRTSGLAPALTPYWNAIGVHQEAEF
                     NRAHVPAFVAGEPAGDFICLYPFVRSLEWYLLEDEERRELLAEHGMLARDYKDVRANT
                     VSTFGLSDYEWLLAFEASEPVRIVQLMRELRAARARLHTREELPFINGIRRPLAEIVK
                     GLE"
     gene            1790..2647
                     /locus_tag="PLE93_03190"
     CDS             1790..2647
                     /locus_tag="PLE93_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNH86084.1"
                     /translation="MLPTFVIALREGVEASLIVGIVAAFLVQQGRRDMLRHVLVGVGI
                     AVLICTGIGIGLQLLNEELPQRQQEMLETVIGLAAVAIVTFMIFWMRRHARGMKSELE
                     SSASRALAEGSAGALIGMAFFAVLREGIETVVFLLAIFQGAEDATAAGLGAVLGILSS
                     IAIGYGIYRGGVRLNLSRFFKLTAVVLVFVAAGLLSNSVHTAWEAGWVTFLQGQAVDL
                     SWLVVPGTWTSALLTGMLGLQSTMTEAQVIVYLVYLLPMLAYVLWPEAWRVRRPRNKV
                     ARAPNPADA"
     gene            2682..>3311
                     /locus_tag="PLE93_03195"
     CDS             2682..>3311
                     /locus_tag="PLE93_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNH86085.1"
                     /translation="MPLRSKHDDRHPLGILLLGFVLLVASAVLLAACGSDESGGSAAQ
                     KIAVEITDEGCQPTDLKAKSGPATFTVTNTASDVNNEFEVLDGALIVGERENLVAGLS
                     GDVSVNLDPGTYDIVCGNPGNREPTGRLVVTGGNVAATTNPVLARAVDEYADFVVEQS
                     NILERRASAFVAAVKAGNVPLAKRLYETSRIPYEKIEPVAESFGTLDPAI"
BASE COUNT          528 a         1081 c         1132 g          570 t
ORIGIN      
        1 aagcgggcgg tcgtcgtcgg agccggcatc gccgggctga ccgcggcctg gcaactcagc
       61 cgatccggcc acgaagtgac ggtgctggac cggaccggcc ggaccggggg ccggatcctc
      121 ccggctgaat tctcggggcg gctggttgac acgggcccgg acgctctcgg tcccgatccg
      181 gcgctggacg aactgctcga acagatcggc atggccggtc aggtcgagat ccccgacccg
      241 gcccgggcct tcaggctgac cgcggcgggt gagctcgagc cctacggggt cggcggcggg
      301 gcgatgttcg gtctcttcgg cgggatcttt tccctgccgg ccaggctgga ggagttgctg
      361 agggaagccg ggcaaaagtt ccgcctcgga gttacggtcg attcggtccg gcaagaggac
      421 cggtcggtgc tcgttgaagc ggaaggatcc gaagaggaat tcgacctggc cgtggtggca
      481 accggtgccc gcgccgcggg gcgcctgctc gacggggaaa cgggcgaacg gctggccgcg
      541 atccggggag tgtcggtcag tctcgttcac ctcgccttcc cggagaaggc gatcggccgc
      601 gagatgaacg gcaccggcta cctcgccgat cccgccgaag gccggctggt caccggctgt
      661 tcctggtctt cggccaagtg gaagcggctg gccggcgacc cggcgatcct gcgggcatcg
      721 atccgtgaag ccggccaggg cgaggtgatc ggtctgccgg accgggaggt tgtggagaag
      781 gtgatggcgg agctggttcc ggtgatggag atcgaaggag atccggtcga gacgatggtc
      841 acccgccacc cggaggccct ggtcgtccgc gacgaggaac atgaatcggc ggtggcggcg
      901 gctcgtggcc ttctggccgc gaccccgcgg atcgggctgg ccggcgccga cctcgacggc
      961 ccgggcatat cgcgctcgat cgccggggtg cgagagactg tcagggcgat cgtcgaaggg
     1021 acacgaaatg actgataccg aagaaacgct ccactactgt gcctaccttt cctattcctg
     1081 ggatgtcgag gccggccggg ggctggcccc ggcggtggaa ccgctcgtgg ctgaaattga
     1141 ggccgccggg gtggagatcc gcggcaccta cgcgacgacc gcgttccgcg ccgaatgcga
     1201 catcctcttc tggctgctgg ccgactcgac cctgaagctc gacgcggcag cccgggcgat
     1261 ccggacctcc gggctcgctc ccgccctgac cccgtactgg aacgcgatcg gcgtccatca
     1321 ggaagccgag ttcaaccggg cccacgtccc cgccttcgtg gccggcgagc cggccggcga
     1381 tttcatctgc ctctatccct tcgtgcgctc gctcgagtgg tatctgctcg aggatgaaga
     1441 gcggcgggag ttgctggccg aacacggcat gctggcccgc gattacaagg acgtgcgggc
     1501 caacacggtc agcaccttcg ggctcagcga ctacgagtgg ctgctcgcct tcgaggcgag
     1561 tgaaccggtc cggatcgtcc agctcatgcg ggagctgcga gcggccaggg cgcgcctcca
     1621 cacccgcgag gaactgcctt tcatcaacgg gataagaaga cctctggcgg aaatcgtcaa
     1681 aggacttgaa taaggggtcg cgagacctcc gcgcgggctc tccccgaggg ctcgacccga
     1741 gccggagtta ggtataccga agtaatcctg tataaacggg ttggttttca tgctgccaac
     1801 ctttgtcatc gcactccgtg aaggcgtcga ggcctctctg atcgtcggga tcgtggctgc
     1861 ctttctggtg cagcagggcc gccgtgacat gctgcgccac gtgctggtcg gagtgggtat
     1921 cgcggtgctc atctgcaccg gcatcgggat cggcctgcag ctcctcaacg aggaactgcc
     1981 gcagcgccag caggagatgc tggaaacggt gatcggcctc gccgcggtgg cgatcgtcac
     2041 cttcatgatc ttctggatgc gccggcatgc ccggggcatg aagtccgaac ttgaaagcag
     2101 cgcctccagg gccttggccg aagggtcggc gggtgccctg atcgggatgg cgttcttcgc
     2161 cgtgctccgc gagggaatcg agacggtggt ctttctgctc gcgatcttcc agggcgccga
     2221 ggacgcgacc gcggccgggc tgggcgcggt gctgggcatc ctctcgtcca tcgcgatcgg
     2281 gtacgggata taccgcgggg gcgttcgcct caacctgagt cgcttcttca agctgaccgc
     2341 ggtggttctg gtcttcgtcg cggccgggct gctctccaac tcggtccaca ccgcctggga
     2401 agcaggctgg gttaccttcc tgcagggtca ggcggtcgat ctcagctggc tggtcgtccc
     2461 cggcacctgg acctcggccc tcctgaccgg gatgctcggc ctgcagtcca cgatgaccga
     2521 agcccaggtg atcgtctatc tcgtctatct gctgccgatg ctggcctatg tgctctggcc
     2581 cgaagcctgg cgggttcgca ggccgcgcaa caaggtcgcg cgcgcaccca acccggccga
     2641 cgcctgagtc tcctccggcc ggtcgccccc gaaaggaatc aatgcccctc cgatcgaaac
     2701 acgacgatcg tcaccccctc ggaattctgc tcctcggctt tgtcctgctt gtcgcttcgg
     2761 ccgttcttct ggccgcctgc ggctccgatg aatccggcgg ttctgcggcc cagaagatcg
     2821 cggtcgagat caccgacgag ggctgccagc cgaccgacct gaaggcaaag tcgggacccg
     2881 ccaccttcac cgtcaccaac accgccagtg acgtgaacaa cgagttcgaa gtcctcgacg
     2941 gtgcgctgat cgtgggcgag cgggagaacc tggtcgccgg tctttccggc gacgtctcgg
     3001 tcaatctcga tcccggcacc tacgacatcg tctgcggcaa ccccggcaac cgcgagccga
     3061 ccggcaggct ggtcgtcacg ggcgggaacg tcgcggcgac gacgaatccc gtactcgcca
     3121 gggccgttga cgagtacgcc gacttcgtgg tcgagcaatc gaacatcctc gagcggcggg
     3181 cgagcgcatt tgtcgccgcg gtaaaggccg gcaacgtgcc cctcgcaaag cggctttacg
     3241 aaacctcgcg catcccctac gagaagatcg aaccggtggc cgaatccttc ggcactctcg
     3301 atccggcgat c
//
LOCUS       DAOQFI010000128         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13885_length_3310_cov_3.468203, whole genome shotgun sequence.
ACCESSION   DAOQFI010000128 DAOQFI010000000
VERSION     DAOQFI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13885_length_3310_cov_3.468203"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLE93_03200"
     CDS             <1..765
                     /locus_tag="PLE93_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HNH86086.1"
                     /translation="KFRPDLAVAHGSVDLAVLSQTFFIPSAQMQDYEFAGLQRKIAWR
                     VAKRVLVPDSIPVERLVKAGARENKLVRFPGLKEDYYLAGFEPDPTVPEALGIDLERV
                     LVIVRPPPETAAYHADNPLYEKVIDRLAGADGVSAVIIPRTEGQRERARARNSTSLIV
                     PDRAVDAQSLIAYADLVVSAGGTMNREAVALGTPVYTTFAGRMGGVDEALIADGSLKL
                     LESAEQLELVKRKDQTGPKKPRDPDFLVQGMLEALA"
     gene            891..1976
                     /locus_tag="PLE93_03205"
     CDS             891..1976
                     /locus_tag="PLE93_03205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNH86087.1"
                     /translation="MVEGIDAVWAFLTAALVTLLLVRPAERLARRVDAIDEPGPRSQH
                     TSPTPRMSGLAILIGIQVAGWIWLPISTETLSILLGACVVSLVGALDDMFDLPAGLKL
                     LGQLGAATIPVVGGVYPANLTVPFFGGFELGWVAYPLTVLGIALVMNIVNLIDGIDGL
                     AAGFCAIAAAAMAIIAVSLQRYEAGVLAAMTAGGAIGFLRYGFPPASSFMGDTGSNLL
                     GYMIAVASVQGALKTNAVIAMVFPLVILAVPLFDTAFVVAKRLKYRQPIFQADRWHFH
                     HRMANIGFSQRRTLLYLYGWTLVLALLALALRFVPYSDDRGNFDPLWTTIMAVVLLAA
                     LAVTVYLAFVLEIIKRPRWRDEQNGED"
     gene            2088..2525
                     /locus_tag="PLE93_03210"
     CDS             2088..2525
                     /locus_tag="PLE93_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86088.1"
                     /translation="MTTYAAKTSLEGEMGNRSNSLIANLDLLVLVLALPVFVIAGFDL
                     AGYAVAAVVWSAQRLIQVWAERQARHKLAAGERTRAMGFVASTSLARPWLMSAVVLVA
                     CIVDREAGLYAAALLVILFTVNLAARGLAFLLGKDEKPEEAAR"
     gene            2522..>3310
                     /gene="atpB"
                     /locus_tag="PLE93_03215"
     CDS             2522..>3310
                     /gene="atpB"
                     /locus_tag="PLE93_03215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNH86089.1"
                     /translation="MSEVATHEQMPDTKTGFSTKAKVFFGIGIWLAVAIAFGLIFGSA
                     GKNEEFKPQNEFKLDPWINIKLGGIDLSINRAVLYLFLASMLTITVMVWIARKMQQKP
                     NRIQTAIEVAYDVTKNDITGNNIDDERLAKKWFPFLASLFFFIFFSNMLGYLPLPTNT
                     HESINIFGLEIPAFALYAATANIAIPLVLTLIVWVSYHVEGIRAKGFIKYLASWLPSG
                     LEDMNPFGKAAIFLIEVVSHFVRLISLSVRLFANILAGHLLLLFM"
BASE COUNT          556 a         1097 c         1005 g          652 t
ORIGIN      
        1 aagttccggc ccgacctggc cgtggcccac ggttccgtgg atctggccgt cctctcgcag
       61 actttcttca ttccctcggc ccagatgcag gactacgagt tcgccggcct gcagcggaag
      121 atcgcctggc gggtggcgaa gcgggtcctg gttccggatt cgatcccggt cgagcgcctg
      181 gtcaaggcgg gagcccggga gaacaagctg gtccgttttc ccggcctcaa ggaggactat
      241 tacctggccg gtttcgaacc cgatccgacc gtcccggaag cgctcgggat cgaccttgag
      301 cgggtcctag tgatcgtgcg gccgccgccc gagactgccg cctaccacgc ggacaatccc
      361 ctctacgaga aggtgatcga ccgcctggcg ggcgcggacg gggtgagcgc ggtaatcatt
      421 ccccgcaccg aaggccagcg ggagcgggcc agggccagga attcgaccag cctgatcgtt
      481 cccgaccggg cagtcgatgc ccagagcctg attgcctacg ccgacctggt ggtcagcgcc
      541 ggcggcacca tgaaccgcga ggcggtcgcc ctcggcaccc cggtctatac gaccttcgcc
      601 ggaaggatgg gcggcgtcga cgaggccctg atcgccgacg gcagcctgaa gcttctcgaa
      661 agcgccgaac aactcgaact ggtcaagcga aaagaccaaa ccggcccgaa gaagccgaga
      721 gaccctgact tcctcgtcca gggaatgctc gaagccctcg cctaggagca ggtcggcacc
      781 tgggttgaag ttattcgctc catgtgcgga taacttcaac ccaaagcctt gggttggcgg
      841 gtttctgttg cgatccggcg aagaccggat cgccatttat tgttcccgac atggttgaag
      901 ggatcgatgc cgtctgggcc ttcctcacgg cggctctggt cactctcctt ctggtgaggc
      961 cggccgagcg gctggcccgg cgggtcgatg cgatcgacga accgggaccg cgcagccagc
     1021 acacgagccc gaccccccgc atgtccggac tggcgatcct gatcggaatc caggtggcgg
     1081 gctggatctg gctgccgatc agcacggaga cgctgagcat cctgctcggg gcctgcgtcg
     1141 tctcactggt cggggcactg gatgacatgt tcgacctgcc ggccgggctc aaactgctgg
     1201 gccagctggg ggcggcgacg atcccggtag tcggcggcgt ctatccggcc aacctgaccg
     1261 ttccgttctt cggcggcttc gaactggggt gggtcgccta cccgctgacc gtgctgggca
     1321 tcgccctggt catgaacatc gtcaatctga tcgacggcat cgacggtctc gccgccggct
     1381 tctgtgcgat cgcggccgcg gcaatggcga tcatcgcggt ctcgcttcag cgttatgagg
     1441 cgggtgtcct cgccgccatg accgccggcg gtgcgatcgg attcctgcgg tacgggttcc
     1501 cgccggcttc cagtttcatg ggggataccg gctcgaacct gctcgggtac atgatcgcgg
     1561 tggcttcggt gcagggagcg ctcaagacca acgcggtgat cgccatggta tttcccctgg
     1621 tcatcctggc ggtgccgctc ttcgacaccg ccttcgtggt cgccaagcgg ctcaagtacc
     1681 gccagccgat cttccaggcg gaccgctggc acttccacca ccggatggca aacatcggtt
     1741 tctcccagcg ccgcaccctg ctctatctct acggctggac cctggtcctg gccctgctcg
     1801 ccctggccct gcgcttcgtc ccctacagcg atgaccgggg caatttcgac ccgctctgga
     1861 ccacgatcat ggcggtcgtc ctgctggctg cgctggcggt aaccgtttac ctcgcctttg
     1921 tgctcgaaat catcaagcga ccccgctggc gcgacgagca aaacggagaa gattgatcaa
     1981 gaaagtacga agtctggttt tcggcttgga taaacggtta gtggcacctg tcactttgcc
     2041 ggaccgcacc gcgccgaccc gcctttcttg gatatagtcg ccgtcccttg acgacgtatg
     2101 ccgcaaagac atccctcgag ggggaaatgg gtaaccgcag caactccttg atcgccaatc
     2161 tggacctttt ggttctggtt ctcgcgcttc ccgttttcgt cattgcggga ttcgatctgg
     2221 ccggctacgc ggtagccgcc gtcgtctggt ccgcccagcg tctgattcag gtctgggccg
     2281 aacggcaggc caggcacaag ctggccgccg gggagcgcac ccgggcgatg gggttcgtcg
     2341 cttcgacctc gctggcccgg ccctggctga tgtccgcggt cgtgctggtc gcctgcatcg
     2401 tcgaccgcga ggccggtctg tacgccgcgg ccctgctggt cattctcttt accgtgaacc
     2461 tcgccgcacg cgggctggct ttcctgctcg gcaaagacga aaagccggag gaagctgccc
     2521 gatgagcgaa gtcgcaaccc acgagcagat gcccgatacg aaaacgggct tcagcaccaa
     2581 ggccaaggtt ttcttcggca ttgggatctg gctcgccgtt gcgatcgcct tcggcctgat
     2641 cttcggttcc gccggcaaga acgaggagtt caagccgcag aacgagttca agctcgatcc
     2701 ctggatcaac atcaagctcg ggggcatcga cctctcgatc aaccgggcgg tcctctacct
     2761 gttcctcgct tcgatgctga ccatcaccgt gatggtctgg atcgcccgca agatgcagca
     2821 gaagccgaac cggatccaga ccgcgatcga agtggcctac gacgtcacca agaacgacat
     2881 caccggaaac aacatcgatg acgagcggct cgcgaagaag tggttcccct tcctagcctc
     2941 gctcttcttc ttcatcttct tctcgaacat gctcggctac ctgccgctgc cgaccaatac
     3001 gcatgagtcg atcaacatct tcgggctcga gattcccgcc tttgccctgt atgcggccac
     3061 cgcgaacatc gcgatcccgc tggtcctgac cctgatcgtc tgggtctcgt accacgtcga
     3121 aggcatccgg gccaagggct tcatcaagta cctggccagc tggctgccct ccggcctgga
     3181 ggacatgaac cccttcggca aggcggcgat cttcctgatc gaagtggtct cccacttcgt
     3241 ccgactgatc tcgctgtcgg tccgactttt cgccaacatc ctcgccggcc acctgcttct
     3301 gctcttcatg
//
LOCUS       DAOQFI010000129         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13926_length_3305_cov_3.128615, whole genome shotgun sequence.
ACCESSION   DAOQFI010000129 DAOQFI010000000
VERSION     DAOQFI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13926_length_3305_cov_3.128615"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLE93_03220"
     CDS             complement(<1..479)
                     /locus_tag="PLE93_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNH86090.1"
                     /translation="MESSAQDQTLKPAEHMTGECMCGKVKFEVDQPLIGAALCYCKRC
                     QRRTGSGVSATGLTQPGSFRITEGEDSVTSYEPGDGGFNKYFCRNCSGQLFTSHPDNP
                     EILAVRLGALNEDPGIRPAAHQFTAYAAPWFPVPDDGLPKFPTAHRRRLPHAPALRTP
                     "
     gene            663..3077
                     /locus_tag="PLE93_03225"
     CDS             663..3077
                     /locus_tag="PLE93_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNH86091.1"
                     /translation="MFGVLFGLGAVLMLLFAMPLAGRADAAKKPPRVTIRTTQYGIPR
                     ILADNPYGLGYGYGWAIASEQLCTLADTYTTVRGERSLYFGGDVNAPNGVTNLDSDFF
                     WKRVRKEGTVQKMINLKPPNGPLPDVKQAVRGYAAGYNAYLRKTGVGKLPDPRCRGKA
                     WVKPISVLDVYMRFYQLLGYGSTDPSMAGIAEAQPPLPMTRKNGSAPADEVNPADITT
                     DDFPKGFGNFTGGLGSNAVALGKNSVKGGGGLLLGNPHFPWHGAQRFFESQLTIPGRL
                     NVSGASLLGVPVILIGHTRTMAWSHTVSTARRFILFREALDPTDPTRYIVDGQSKPMK
                     ATTVRVKDKDGIVHTRTLYSTEHGYMTNQLQGSNLFGWATDYGYSLYDANTGNFRMIN
                     HFYSMNQARSVPEVLKILKKYQGIPWVNTIASDSKGRALYADIGAVPNVTDGRVADCN
                     VGLGIVTWPSYRVPIFEGSDSNCDMSKKAPGAAGPGILPPSQMPYQVRSDYTENSNDS
                     YWFTNSKHTLDGFPLIIGQEGIAQSERTRLGHKMVGEKLAGDGRFTLASLKAMEFNDR
                     VMSAELLLDPVIDYCNANPSVVGSGGPVSTASACDALEAWDGRNNLDSKGGLFFQRFA
                     GRLYSNSTDPVYANPFDIADPIATPNGLNTLNSALPIALADTITEFQDQGIPLTATRR
                     KYQTVTRNGVKIPIHGGDYEPYGSFNTIWGPWIPGKGITEVSDGSSFIQAVRLDGSKC
                     PRASMILTYSQSENPKSKHYADQTKLFSKKGWSLDRFCPNQQKKSPGLKVKRFGGGAK
                     AESRGY"
     gene            3091..>3305
                     /locus_tag="PLE93_03230"
     CDS             3091..>3305
                     /locus_tag="PLE93_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNH86092.1"
                     /translation="MEQLAVEVGDRRVLDAIAAVPRDLFVPEDQRRFAWENRPLPIGR
                     GQTISQPLVVATMCELLELSEDDLVLDV"
BASE COUNT          610 a         1076 c         1075 g          544 t
ORIGIN      
        1 ggagtgcgaa gagcgggcgc atggggcaga cggcggcggt gtgcggtggg gaatttgggc
       61 aggccgtcat ccggcaccgg gaaccagggc gcggcatagg cggtgaattg gtgggcagcg
      121 gggcggatgc ccgggtcctc gttgagggcg ccaagccgga ccgcgaggat ctccgggttg
      181 tcgggatgcg aggtgaaaag ctggccgctg cagttgcggc agaagtactt gttgaagccg
      241 ccgtcgccgg gctcgtaact cgtgacggaa tcctcgcctt cagtgatccg gaacgagccc
      301 ggctgggtca gtccggtggc cgagacgccc gatccggtgc ggcgctggca gcgtttgcag
      361 tagcagaggg ccgccccgat caggggctgg tcgacctcga acttcacctt gccgcacatg
      421 cactcgccgg tcatgtgttc ggccggtttc aaagtttgat cctgcgctga gctctccatg
      481 atccaactct agtccgaacg gagccgcccc cagccattca gggcttcaaa cgggagggcg
      541 ggctctcgcc ggggtccgca cagaccggac cgactgcagt tagtcttttc gcgagggaaa
      601 caaccgaagg agagtcaatg atgcaggtgt cttcgtccgg ggcaatgcgt cccctgggcc
      661 gtttgttcgg ggttctgttc gggcttggcg cggtactgat gctccttttc gccatgccgc
      721 tggcgggccg ggccgacgcg gcgaagaagc cgccgcgggt cacgatccgc accacccagt
      781 acgggatccc gcggatcctc gccgacaacc cctacgggct cggctacggc tacggctggg
      841 cgatcgccag cgagcagcta tgcacgctag ctgacaccta caccacggtt cggggcgagc
      901 ggtcgctcta cttcggcggc gacgtcaacg ccccgaatgg cgttacgaac ctcgactcgg
      961 atttcttctg gaagcgggtg aggaaggagg gcaccgtcca gaagatgatc aacctgaagc
     1021 cgccgaacgg gccgctgccc gacgtcaagc aggcggtcag gggatacgcc gccggttaca
     1081 acgcctacct caggaagacc ggggtaggga agctgcccga cccgcgttgc cgcggcaagg
     1141 cctgggtcaa gccgatctcg gtgctcgacg tctacatgcg cttctaccaa ctgctcggtt
     1201 acggctcgac cgacccctcg atggccggca tcgccgaggc ccagccgccg ttgccgatga
     1261 cgaggaagaa cggctccgcc ccggccgatg aagtcaatcc cgccgacatc actaccgatg
     1321 atttcccgaa gggcttcggc aacttcaccg gcggtctcgg ctcgaacgcg gtcgccctgg
     1381 gcaagaactc ggtcaagggc ggcggcggcc ttctgctcgg caacccgcac ttcccctggc
     1441 atggtgccca gcgcttcttc gagtcgcagc tgaccatccc cggccggctc aacgtttccg
     1501 gggccagcct gctcggggtg ccggtgatcc tgatcggcca cacccgcacc atggcctgga
     1561 gccacacggt ttcgaccgcc cgccgtttca tcctcttccg ggaggcgctc gacccgaccg
     1621 acccgacccg ctacatcgtt gacggccagt cgaagccgat gaaggcgacc acggtcaggg
     1681 tcaaggacaa ggacgggata gttcacacca ggaccctcta ttcgaccgag cacggctaca
     1741 tgaccaacca gctccagggc agcaacctgt tcggctgggc gacggattac ggctactcgc
     1801 tctatgacgc gaacaccggc aacttccgga tgatcaacca cttctactcg atgaaccagg
     1861 cccggagcgt gcccgaggtc ctgaagatcc tgaagaagta ccagggcatc ccttgggtca
     1921 acacgatcgc ttccgattcg aagggccggg cgctttacgc ggacatcggc gcggtaccga
     1981 acgtcacgga tggacgagtc gccgattgca acgtcggcct cggcatcgtg acctggccgt
     2041 cctaccgggt gccgatcttc gaaggttcgg attcgaactg cgacatgagc aaaaaggcgc
     2101 ccggtgcggc cggtcccgga atcctgccgc ccagccagat gccgtatcag gtgcgctccg
     2161 actacacgga gaactcgaac gactcctact ggttcaccaa ctccaaacac accctcgacg
     2221 gcttccccct gatcatcggt caggaaggga tcgcccagtc cgagcggacc cggctcggcc
     2281 acaagatggt cggcgagaag ctcgcgggcg acggcaggtt caccctcgcc tcgctcaagg
     2341 caatggagtt caacgaccgg gtgatgagcg cggagcttct gctcgacccg gtgatcgatt
     2401 actgcaacgc caacccgtcg gtggtcggga gcggcgggcc ggtgtcgacg gcaagtgcct
     2461 gcgacgcgct cgaggcatgg gacggccgca acaacctcga ctcgaagggc ggtctgttct
     2521 tccagaggtt cgccggccgg ctctactcca acagcacgga cccggtctac gcgaatccct
     2581 tcgacatcgc cgatccgatc gccaccccga acgggctgaa cacgctcaat tcggcgctgc
     2641 cgatcgcatt ggccgacacg atcaccgagt tccaggatca gggcatcccg ctgaccgcga
     2701 cccgccgcaa gtaccagacg gtgacccgca acggggtgaa gatcccgatc cacggcgggg
     2761 attacgagcc ctacggttcg ttcaacacga tctggggccc ctggattccg ggcaagggca
     2821 tcaccgaggt cagcgacggc tccagcttca tccaggcggt tcggctggac ggttcgaagt
     2881 gcccgcgggc gagcatgatc ctgacctact cgcagtcgga gaacccgaag tcgaagcatt
     2941 acgccgacca gacgaagctc ttctcgaaga agggctggtc gctggatcgc ttctgcccca
     3001 accagcagaa gaagtcaccg gggctcaagg tgaagcgatt cggcggggga gcgaaggcag
     3061 aaagtcgcgg gtactgagcg ggatcggctg atcgaacagc tggcggtcga ggtcggggac
     3121 cgcagggtcc tcgacgcgat cgccgctgtt ccccgcgatc tcttcgttcc ggaggaccag
     3181 cgccggtttg cctgggagaa ccgcccgttg ccgatcggcc ggggccagac gatctcccag
     3241 ccactggtcg tggcgacgat gtgcgagctg ctcgagctga gtgaggacga cctcgtcctc
     3301 gacgt
//
LOCUS       DAOQFI010000130         3301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_13955_length_3301_cov_3.202403, whole genome shotgun sequence.
ACCESSION   DAOQFI010000130 DAOQFI010000000
VERSION     DAOQFI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13955_length_3301_cov_3.202403"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1013
                     /locus_tag="PLE93_03235"
     CDS             345..1013
                     /locus_tag="PLE93_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH86093.1"
                     /translation="MNTSIAEDSVSPEELTEAVSERRGWKLYQVIRLILRPLRWWARV
                     ESKGLEYLPATGPALIVSNHDSWLDPLAIIEVMMWKKRQLRFLAKHTLWKFRPMAWIL
                     DQAAQIPIRRGEGDTGALDAAAVAVERGEMIGIFPEGTLSRGTVLRARKGVSRLARAC
                     PGVPVVLVAVTGSSDLKRFPKRPRVKVEFFDPAGGQLRPDEDAQEFSQRLLDEIRTKA
                     PRVK"
     gene            complement(1023..1448)
                     /locus_tag="PLE93_03240"
     CDS             complement(1023..1448)
                     /locus_tag="PLE93_03240"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH86094.1"
                     /translation="MQRMRFDKDFDLPPERVFDFIAEHENLGGVLGAEVTRLSYGTDG
                     NRNGVGSSRKLNPPGPVPAFEETVTEFEPHSRIVYTVTKGTPLNHHLGTITFAPTDSG
                     THMEWNIEIGTRLPGLDFVIAKVLVRNIGGGLDRMTIPA"
     gene            1505..1873
                     /locus_tag="PLE93_03245"
     CDS             1505..1873
                     /locus_tag="PLE93_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86095.1"
                     /translation="MTKREPKGESGAEARRQALKSGGAAVVTHRKPRAPRSDGPRTTL
                     RVPDSLVISAERLAGELGISRNDALLRLATRGADLYETERLIEKRREERWKAVVPGEI
                     EPGVSFPEAEEIAEAVRPGL"
     gene            2006..2368
                     /locus_tag="PLE93_03250"
     CDS             2006..2368
                     /locus_tag="PLE93_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86096.1"
                     /translation="MFYGIELKAMSGDRRFGVLRDWLVRLIGSQVLNVISLDGRAAVI
                     AGRLRAENPHPPPGRDSRSKTMRQAAWLFDIQIAAVAFAAGLDVATRNIRDFEVLSAS
                     LERLYPGAPTLAVYPDFR"
     gene            complement(2365..>3301)
                     /locus_tag="PLE93_03255"
     CDS             complement(2365..>3301)
                     /locus_tag="PLE93_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH86097.1"
                     /translation="IGICEHAFWEAITHADSRVLYGMKGTDFPQVRQAFTDAFARTAA
                     MKLFAWRAVDYFRTASPEDRRYLLFNPLTKMKVTTEGEKVVDLLWDVIAAKGFERDMW
                     FEMAARDIRALPKLEGTVHVNMALVLKFMASYLFNPDPELAAPERRQDPGDDEFLFRQ
                     GPARGLGKIRFSPWRESYIRFASVPNVAKFMELAESFKAFLVEAGPDEAQQKDLDFLL
                     TLGEIFTLIPYGQLICEQAALIGLPEDTLDQIFDVMIRDFSAYAAELHGKTSSTEAQQ
                     EWARGAITKPVVDQARFDSVWEKTRALAGGYEMNP"
BASE COUNT          570 a         1023 c         1107 g          601 t
ORIGIN      
        1 gaacttcccg gtccgggcct ccgggacctc cagcccgctc cgtccgggaa gcccccggcc
       61 tgatcttcaa cagcaccggg accgcaagct agactgccgc gggtggagcg ggagaaaggg
      121 gaggccgtga gcgggcgacc gctgaccaga cgcgcgctac tcgggcgagg tgcggcgctg
      181 gctgctgccg gagccttcgc accggccttt cggatcgatc cggcgagtgc cgccgcgacc
      241 tgttcaccgc ccgtttcggg tgatttccgc gaacaccgca gtcttcagag tctcaggctc
      301 tagagtccga tgttcatgaa tccgcaaggc acggcgggaa gccgatgaac acatcgatcg
      361 ccgaggattc ggtctcgcca gaagagctga ccgaggccgt ttccgagcga cgcggctgga
      421 agctctacca ggtcatccgc ctgattctgc ggccgttgcg ctggtgggca cgggtggaat
      481 cgaaggggct cgagtacctg ccggcgaccg gtcccgcgct gatcgtctcg aaccacgatt
      541 cctggcttga cccgctggcg atcatcgagg tgatgatgtg gaagaagcgt caactgcgtt
      601 tcctcgccaa gcacacgctc tggaagttca ggccgatggc ctggatcctg gatcaggccg
      661 cgcagatccc gatccggcgc ggcgagggtg acaccggcgc cctggacgcc gcagcagttg
      721 ccgtcgaacg cggtgagatg atcggcatct tccccgaagg aacgctttca cggggcacgg
      781 tgctgcgagc ccgcaaggga gtctcccggc tggcccgggc ctgcccggga gtcccggtcg
      841 tgctggtcgc ggtgaccggc agcagtgacc tgaagcgttt ccccaagcgg ccccgggtga
      901 aggtcgagtt cttcgacccg gccggggggc agctgagacc ggatgaggac gcgcaggagt
      961 tctcgcagcg cctgctcgac gagatccgca ccaaggcgcc tcgcgtcaag tagcgccggg
     1021 gttcaggccg ggatcgtcat ccggtcgagt ccgccgccga tgttcctgac cagcaccttg
     1081 gcgatcacga agtcgaggcc gggcaggcgg gtgccgatct cgatgttcca ttccatgtgg
     1141 gttccggaat cggtcggggc gaaagtgatc gtgccgaggt gatggttgag cggggtgccc
     1201 ttggtcaccg tgtagacgat ccggctgtgc ggctcgaatt cggtcaccgt ctcctcgaag
     1261 gccggaaccg gcccgggcgg attcagcttc cgcgaggaac cgactccgtt gcggttgccg
     1321 tcggtgccgt aggaaagccg ggttacctcg gctccgagga ccccgcccag gttctcgtgc
     1381 tcggcaatga agtcgaagac ccgctcgggc ggaaggtcga agtccttgtc aaatcgcatg
     1441 cgctgcatgg ccggattgaa tcagattcgc ggtagcgcat caactgatgc gttatgatcg
     1501 agacatgacg aaacgtgaac cgaaaggcga atccggagcg gaggcccgtc ggcaggcgct
     1561 gaaaagcggt ggcgctgccg tggtcaccca ccgcaagcca agagcgccgc gttccgatgg
     1621 tccgcgtact actttgcggg ttcctgactc gttggttatt tcagccgaac ggctcgccgg
     1681 cgaactcggc atttcccgca atgatgccct gctccggctc gccacccggg gagctgacct
     1741 gtacgaaaca gaaaggctta tcgaaaagcg gcgtgaggag cggtggaaag cggtggttcc
     1801 aggtgaaata gagccgggag tcagcttccc cgaggcagag gaaattgccg aagcggtccg
     1861 tcccgggctt tgagttttgg tggaccgctt gtgggacacc acattcgctt ctcgtatccg
     1921 cccggatagc agccttttcg actacgcggt ctccgaatca cgccagggta gaccggtacc
     1981 gatcgcagca ccctcgctca ctgagatgtt ctacgggatc gagttgaagg ccatgtcagg
     2041 cgatcgtcga ttcggtgtcc tgcgggactg gctggttcga ttgatcggct cccaggtgtt
     2101 gaacgtgatt tcactggacg ggcgggccgc cgttattgcc ggtcgacttc gggccgagaa
     2161 tccccacccc cctccgggcc gtgattcaag gtccaaaacg atgagacagg cagcctggct
     2221 gttcgacatt cagatcgccg cggtcgcttt cgctgccgga ctggatgtcg ctacccggaa
     2281 catccgtgat ttcgaggtac tgagcgcgtc gcttgagcgg ctctaccccg gcgcaccgac
     2341 gctggctgtc taccccgact ttcgctaggg attcatctcg tagccgccgg ccagggcgcg
     2401 ggtcttttcc cagaccgagt cgaacctggc ctgatcgacg accggcttgg tgatcgcgcc
     2461 acgggcccac tcctgctggg cctcggtcga ggaggtcttg ccgtggagct cggccgcgta
     2521 ggccgagaag tcgcggatca tcacgtcgaa gatctggtcg agcgtgtcct cgggcaggcc
     2581 gatcagcgcc gcctgttcgc agatcagctg gccgtacggg atcagggtga agatctcgcc
     2641 gagggtgagc aggaaatcga ggtccttctg ctgggcctcg tccggccccg cctcgaccag
     2701 gaatgccttg aacgattcgg ccaactccat gaacttcgcc acgttcggca ccgaggcgaa
     2761 gcgtatgtat gactcccgcc agggactgaa gcgaatcttc ccgaggccgc gggcggggcc
     2821 ctggcggaag aggaactcgt catcacccgg atcctggcgg cgctccggtg cggcgagttc
     2881 ggggtccgga ttgaacaggt aggaggccat gaacttgagg acgagagcca tgttcacgtg
     2941 gaccgtgccc tccagcttcg gcagcgcgcg gatgtcgcgg gcggccatct cgaaccacat
     3001 gtcccgttcg aaccccttgg ccgcgatcac atcccagagc aggtcgacca ccttctcgcc
     3061 ctcggtcgtg accttcatct tggtcagtgg gttgaagagc aggtagcgac gatcctcggg
     3121 ggaagcggta cggaagtagt ccaccgcccg ccacgcgaag agtttcatcg cggcagtccg
     3181 ggcgaaggcg tcggtgaagg cctgccggac ctgggggaag tcggtcccct tcatcccgta
     3241 gagaacccgg ctgtcggcgt gggtgatcgc ctcccagaag gcgtgttcgc agatcccgat
     3301 c
//
LOCUS       DAOQFI010000131         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_14000_length_3295_cov_3.051235, whole genome shotgun sequence.
ACCESSION   DAOQFI010000131 DAOQFI010000000
VERSION     DAOQFI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14000_length_3295_cov_3.051235"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLE93_03260"
     CDS             <1..697
                     /locus_tag="PLE93_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNH86098.1"
                     /translation="VPGGQPVRVMTINLGVGYAHADEIAELARVRDVDLVAVQELTPA
                     EASGLDRAGFATDYPHRILQPGTEASGGGIYSRWPLVDRGSLTAEFHQPVADVRIPAS
                     IPIEFVSVHPMAPTTPARTSQWASEYKSLPEASSARLPLVLAGDFNATLDHRNLRDLI
                     DTGYRDAAEVTGSGLVTTWPSRIEWKLPVTLDHVLAEKGISFSQYDVEKVAGTDHRAV
                     IVELVMPPVPGGR"
     gene            complement(716..1039)
                     /locus_tag="PLE93_03265"
     CDS             complement(716..1039)
                     /locus_tag="PLE93_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86099.1"
                     /translation="MNPSTALKSGLVMLALVAVLAVLLAVALPKSFFEDWGWFSGPAA
                     LFLCAFGTARILGLPVGRTLLGVFIAGIPGLIGVVTGVHWLGTLLAVLTFAVWCGYGP
                     EPRRA"
     gene            1096..2328
                     /locus_tag="PLE93_03270"
     CDS             1096..2328
                     /locus_tag="PLE93_03270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNH86100.1"
                     /translation="MTRSSGIFRSGDRLRQQVSLLFFFLLIAGLAILIPSHASSADQN
                     PIRSSRLSQKGSSLIVQIRTSQKVNLGRLDRRPDFANGNHRYLCLQMNPRGGTTIVRI
                     CLGGRSRTYRVLGVSRTSEAGRVRSSKVIRAEVKRVGQLKLVATFDPAAAGLPPGNYA
                     WRIADHSGECPPSGPKARPTEECLSYFPPKKRAVYRLRPIRVVGCTGGNGELVRNGPR
                     GGKRVALTFDDGPSDYTPEVLHILKRKEVKATFFMLGQQVTRYPSYARRVLALGHEIG
                     NHSYDHALLPVSSDIRHTNRSIIQATHFRPCLFRPPYGAVSSSLVASARAARMKVVDW
                     DVDTTDWKLPGAGYIKSNVLGNAHSGSIVLMHDGGGPRSQTVEALPGIIDGLRGRGYR
                     LVTVSQLLGNRMIYRPVP"
     gene            complement(2389..>3295)
                     /locus_tag="PLE93_03275"
     CDS             complement(2389..>3295)
                     /locus_tag="PLE93_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86101.1"
                     /translation="VMTSALQNLSHFNVLADRLAQGLINELFLARLMYHPNGLGTAPA
                     FQDGDGFTAGQSLIKTDHVYYMGASQGGIMGGPLTALSPDFTQSSLLVGSMNYSVLLP
                     RSIDFDLYSAILYPSYPGDLAHPLLFQLMQMLWDRSEPNGYAHVMTDNPPPDTPEHKV
                     TLMIAVGDHQVSNYASEVMARTLGMSTNQTPVDEGRWPDYDILWNVPRLSASDYPFHG
                     NNVIYFDGGPPRPEPGNPSKTIGTDVPPFANVPNRSAQDPHGAPGGASVAVEMTSTFL
                     QPNGYISDVCSPHACYGDAWDGSLP"
BASE COUNT          570 a         1076 c         1120 g          529 t
ORIGIN      
        1 cgtcccgggc ggtcaaccgg tgcgggtgat gacgatcaac ctcggggtcg ggtacgccca
       61 cgccgatgaa atcgccgagc tcgcccgggt ccgggacgtc gacctcgtcg cggttcagga
      121 actgaccccg gcagaagcct ccgggctcga ccgggccggc ttcgcgaccg actatcccca
      181 ccggatcctt cagccgggga ccgaagcgtc cggcggaggc atctactccc gctggccgct
      241 ggtcgaccgg ggaagtctga ccgccgagtt ccaccagccg gtggccgacg tgcggattcc
      301 cgcctctatc ccgatcgaat tcgtctcggt tcacccgatg gccccgacca cgccggcccg
      361 gacctcgcag tgggcctccg agtacaagtc attgccggaa gcctccagcg ccaggctgcc
      421 cctggttctg gccggggact tcaacgcgac tctcgatcac aggaaccttc gcgacctgat
      481 cgacaccggc taccgcgacg ccgccgaggt gaccggcagc ggcctggtta cgacctggcc
      541 gtcgcggatc gaatggaaac tgcccgtgac cctcgaccat gtgctggccg aaaagggcat
      601 ttcgttcagt cagtacgacg tggagaaggt cgcgggcacc gatcaccggg ccgtgatcgt
      661 cgagctggtc atgcctccgg tgcccggcgg ccggtgagcg ccggcacctg aaccttcagg
      721 cgcggcgggg ttccgggccg tagccgcacc agacggcgaa ggtgagcacg gcgagcaggg
      781 tgccgagcca gtggactccc gtgaccacgc cgatcaggcc ggggatgccg gcgatgaaca
      841 ccccgagcag cgtgcggcca accggcaggc cgaggatccg ggccgtgccg aaggcgcaga
      901 gaaacagtgc ggccgggccg gagaaccagc cccagtcctc gaagaatgac ttcggcagcg
      961 ccacggcgag cagcaccgcc agcaccgcca ccagggcgag catgacgagg ccggacttga
     1021 gggcggttga cggattcatg acggcagacg gccgactcgg gaaggccacg ccggagccta
     1081 tactgggagc gcatcatgac ccggtcgtca ggcatatttc ggtcggggga caggcttcgc
     1141 caacaggtct cactgctgtt cttcttcctg ctcatcgcag ggctggcgat tctcatccct
     1201 tctcacgcca gcagcgccga ccagaacccg attcgctctt ccaggctgtc ccagaagggg
     1261 agctcgctga tcgtccagat caggaccagc cagaaggtga acctgggcag gcttgaccgc
     1321 cggcccgact tcgcgaacgg caaccaccgc tatctctgcc tgcagatgaa tccccgcggc
     1381 gggaccacga tcgtgcggat ctgcctcggc gggcgaagcc gcacctaccg ggtgcttggc
     1441 gtctcacgga cttcggaggc cggccgggtg cgttcgagca aggtgatccg ggcagaagtg
     1501 aaaagggtcg ggcagctgaa actcgtcgcc actttcgacc cggcggctgc cggtctgcct
     1561 cccgggaact atgcgtggcg gatcgccgac cattccggcg agtgcccgcc gtccgggccg
     1621 aaggcgaggc cgaccgaaga atgcctttcg tacttccccc cgaaaaagcg tgcggtctac
     1681 cggcttcggc cgatccgggt ggtcggctgc acgggtggca acggcgagct ggtgcgaaac
     1741 ggcccgcgcg gcggcaagcg agtggccctg accttcgatg acggtccgag cgactacacc
     1801 ccggaagtcc ttcacatcct caagcgcaag gaagtcaagg cgaccttctt catgctgggc
     1861 cagcaggtaa ccaggtatcc gtcatacgcc cgacgggttc tggcgctggg ccacgagatc
     1921 ggcaatcaca gctatgacca cgccctgctt ccggtcagct ccgacatccg tcacaccaac
     1981 cggagcatca tccaggcgac ccacttccgg ccctgcctgt tccgaccgcc ctacggcgcg
     2041 gtcagcagct cgctcgtggc ctcggcccgg gcggcccgga tgaaggttgt cgactgggat
     2101 gtcgacacaa ccgactggaa gctgcccggt gccgggtaca tcaagtcgaa cgtcctcggt
     2161 aacgcgcatt cagggtcgat cgtcctgatg catgatggcg gcggcccccg cagccagacg
     2221 gtggaggcgc tgcccggaat catcgacggg ctccgcgggc ggggttaccg gctggtcacg
     2281 gtctcgcagc tcctcggcaa ccggatgatc taccgcccgg tcccctgaaa agcgaaagcc
     2341 ccgggcgttt ccgcaccggg gctttctctc ggaatccgtt tctgccggtt acggcaggct
     2401 gccgtcccag gcgtcgccgt agcaggcgtg gggtgagcag acgtcactga tgtagccgtt
     2461 cggctgcagg aaggtcgagg tcatttcgac cgcgacgctg gccccgccgg gagctccgtg
     2521 cggatcctgg gccgaacggt tcggaacgtt ggcgaagggg ggcacgtcgg tgccgatcgt
     2581 cttcgacgga ttgcccggct ccggtcgcgg cggtccacca tcgaagtaga tgacgttgtt
     2641 gccgtggaac ggatagtcgg acgcggacag cctcgggacg ttccagagga tgtcgtaatc
     2701 gggccagcgt ccttcatcga ccggcgtctg gttggtgctc atgccgaggg tccgggccat
     2761 cacttccgag gcgtagttgg agacctgatg gtcgccgacc gcgatcatca gggtgacctt
     2821 gtgctcgggg gtgtccggcg gcgggttgtc ggtcatcacg tgggcgtaac cgttgggctc
     2881 gctgcggtcc cagagcatct gcatcagctg gaacaggagc ggatgggcca gatcgcccgg
     2941 gtaggacggg tagaggatcg ccgagtagag gtcaaagtcg attgaacgcg gcagcagcac
     3001 cgagtagttc atcgaaccga ccagcagcga ggactgggtg aagtcgggcg agagcgcggt
     3061 cagcggaccg cccatgatcc cgccctggct ggcgcccatg tagtagacgt gatcggtctt
     3121 gatcagcgac tgtccggccg tgaaaccgtc accgtcctgg aaggccggag cggtgccgag
     3181 gccattcggg tggtacatca gacgggccag gaacagctcg ttgatcaggc cctgggccag
     3241 ccggtcggcg agcacgttga agtgcgacag gttctgcagg gcgctggtca tcacc
//
LOCUS       DAOQFI010000132         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_14278_length_3257_cov_2.463148, whole genome shotgun sequence.
ACCESSION   DAOQFI010000132 DAOQFI010000000
VERSION     DAOQFI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14278_length_3257_cov_2.463148"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PLE93_03280"
     CDS             complement(<1..822)
                     /locus_tag="PLE93_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH86102.1"
                     /translation="MSENPAGAAVDAGPGIPPPFTREHEELRETVRRFVENEIAPHVD
                     EWEDARLFPRELFDRCGELGFLGLKFPEEYGGEGGSYLHDAIWVEELSRRGASGGVAA
                     GINAHASIAMPPVFKFGDEWQRRQWLVPGIRGEKIGALGITEPGAGSDVASIRTFARK
                     VDGGYVVNGSKTFITNGVRADFLVCAVKTTEKGGHNGISFLVIDTDSPGYEVVTKLEK
                     LGWHASDTGEISFTDVEVPEEHLLGDENEGFRLIMANFAWERLLMAIGAVGAMDAL"
     gene            complement(815..1786)
                     /locus_tag="PLE93_03285"
     CDS             complement(815..1786)
                     /locus_tag="PLE93_03285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH86103.1"
                     /translation="MEKRKLGSEGLEVSAIGLGCMGMSEFYGEGDEAESIATIHRALE
                     VGVNFLDTADMYGPWTNEELVGKAISDRRDQVVLATKFGIRRGDDPRDRHVDGRPEYV
                     RESIDGSLKRLGVDHVDLYYQHRVDKTVPIEETVGAMAELVEAGKVRYLGLSEASAES
                     IRKANSVHPISALQTEFSLFERGASDKVIPTLRELGIGFVPYSPLGRGFLTGRFRSGA
                     DFGSGDFREHDPRMSQENMAANMAIVEAIEDLASRHDVSPAQMAIAWVLAQGDDFVPI
                     PGTKKVSRLEENAAAAEVSLSDEELASLDALPRPQGDRYANMASIDE"
     gene            complement(1946..2512)
                     /locus_tag="PLE93_03290"
     CDS             complement(1946..2512)
                     /locus_tag="PLE93_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86104.1"
                     /translation="MNTSRAFAVAAAFAATAFIAFFSFAGHAQAAGCKDASSGGLTLA
                     PADCKPVKKARLIRGIAYAPDNAPAAVKRVIAAANRIRLKPYIYGGGHTLSRRLQAGY
                     DCSGSVSYALRAGGFISYPMPSGSFTNWKQPGAGKWITTYANGGHMYMIVAGLSFDTS
                     NMSSSGGNRWSTSIRSSRGFSVRHPGGF"
     regulatory      complement(2550..2695)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            3007..>3257
                     /locus_tag="PLE93_03295"
     CDS             3007..>3257
                     /locus_tag="PLE93_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH86105.1"
                     /translation="MDIAGKKVLLTGATGGIGRRIASTLAAAGAEVIVSGRSAEALTE
                     LVDSLPGSGHSVLAADLGLVGAAQELASQAGAVDMLVANA"
BASE COUNT          530 a         1057 c         1084 g          586 t
ORIGIN      
        1 cagggcgtcc atcgccccca cggcgccgat cgccatcaac agccgctccc aggcgaagtt
       61 ggccatgatc aggcggaagc cctcgttctc gtcacccagc agatgttctt ccggaacctc
      121 cacgtcggtg aaggagatct cgccggtgtc cgaggcatgc cagccgagct tctccagctt
      181 ggtcaccacc tcgtaaccgg gtgagtcggt gtcgatgacc aggaacgaga tcccgttgtg
      241 gccacccttt tcggtggtct tgacggcgca gaccaggaag tcggcccgga ccccgttggt
      301 gatgaaggtc ttcgatccgt tgaccacgta gccgccatcc accttccgcg cgaaggtgcg
      361 gatcgaggcc acgtcggaac cggcgccggg ctcggtgatc ccgagggcac cgatcttctc
      421 gccgcggatc ccggggacca gccattgccg gcgctgccat tcatccccga acttgaagac
      481 cgggggcatg gcgatcgagg cgtgcgcatt gatcccggcg gcgactcctc cggaagcgcc
      541 gcgccgggaa agctcctcga cccagatcgc gtcgtgcagg tagctgccgc cctcgccgcc
      601 gtactcctcg gggaacttca gaccaagaaa gccgagctcg ccgcagcggt cgaagagctc
      661 gcgggggaaa agccgggcgt cctcccactc gtcgacatgg ggggcgatct cgttttcgac
      721 gaagcgccgt acggtctcgc ggagttcctc atgttcccgg gtgaagggcg gcgggatgcc
      781 gggtcccgca tcaaccgccg ccccggccgg gttttcactc atcgatcgaa gccatgttgg
      841 cgtagcggtc accctgcggc cgcggcaggg cgtccagcga ggccagctcc tcatcactca
      901 ggctgacctc cgccgcagcc gcgttctcct cgaggcggct gaccttcttg gtgccgggga
      961 tcggcacgaa atcgtcgccc tgggcgagga cccaggcgat cgccatctgg gcgggggaga
     1021 cgtcgtgacg gctggcgaga tcctcgatcg cctccacgat cgccatgttg gcggccatgt
     1081 tctcctggct catccgcggg tcgtgctcac ggaagtcacc gctgccgaag tcggcaccgg
     1141 aacggaaccg cccggtcaga aagccgcggc ccagggggga gtagggaacg aagccgatcc
     1201 cgagttccct cagggtcggg atcaccttgt cgctcgcgcc ccgctcgaag agcgaaaact
     1261 cggtctggag cgccgagatc ggatggaccg aattggcctt ccggatgctt tcggccgagg
     1321 cttcggaaag tcccagatag cggaccttgc ccgcctcgac cagctctgcc atcgctccga
     1381 ccgtctcctc gatcggcacg gtcttgtcga cccggtgctg gtaatagagg tcgacgtgat
     1441 cgaccccgag ccgcttcagc gagccgtcga tcgattcccg cacgtactcg ggccggccgt
     1501 cgacatgccg gtcgcggggg tcgtctcccc ggcggatccc gaacttggtc gccagcacca
     1561 cctgatcgcg ccggtcggag atcgccttgc cgaccagttc ctcattggtc cagggcccgt
     1621 acatgtcggc ggtgtcgagg aaattgactc cgacctccag ggcccggtgg atggtcgcga
     1681 tcgattccgc ttcgtcgcct tcaccgtaga actcggacat gcccatgcag cccagcccga
     1741 ttgcggaaac ttcgagccct tcgctgccca gcttgcgttt ctccatctgt cgatcataga
     1801 accggcgcgc tccccggccc cctcgtcccc ggaatcatgg ccggtcgacc ccgtcgtggc
     1861 acgccgggcg ggaagggaac gggttgatgc aaaagaaacg ccccggactt gcggggcgtt
     1921 tcgcgattct gctgtgtcgg caggtctaga agccgccggg gtgcctgacg ctgaatcccc
     1981 tggacgaccg gatcgaggtg ctccagcgat tgccgccgga ggagctcatg tttgaagtat
     2041 cgaacgagag gccggccacg atcatgtaca tgtgaccgcc attggcgtag gtggtgatcc
     2101 acttgccggc accgggctgc ttccagttgg tgaagctgcc ggaaggcatc gggtagctga
     2161 tgaagccgcc ggcgcggagc gcgtagctga ccgagccgga gcagtcatag ccggcctgca
     2221 gcctgcggct gagggtgtga ccgccaccgt agatgtacgg ctttaggcga atccggttgg
     2281 cggcggcgat gacccgcttg acggcggcgg gagcgttgtc cggggcgtag gcgatgcccc
     2341 ggatcagccg ggccttcttg accggcttgc agtcggccgg ggcgagggtg agcccgccac
     2401 tcgaggcgtc cttgcagccg gcggcctggg cgtggccggc aaaggagaag aaggcgatga
     2461 aggcggttgc ggcaaatgcg gcggcaacgg cgaaagcccg ggaagtattc aaagcaactg
     2521 acgtctgatt tttgtgaaac aggttgatac cgaactcctt ggtcggccta cggggttagc
     2581 tgtcgggctg gcgctttagg agccgcgccc ccacctgaat cagtgggttc gccccagaaa
     2641 cttggttccc ccgatcccgg tcacagagtg agtcgggatt cggcgtctcg gttatgaact
     2701 tgcgggatca tagacgatct gcatgtcatt cgctaacaaa ccccccgatc cctttgttca
     2761 tccctgcagt ttccatacat aagtagtcgg cgagcttcgc cccgcctcca tgctcaaccc
     2821 gccggcccag gacacccggg aacggatggg ccggctccgt ttggtgaagc ttcaggcctg
     2881 atcaccctgg aggcacttcc gctggagtcc cgcccaacca ttctttgctt cgagtgggcg
     2941 gggcgggttt ctctgggagg gcgggttgga ccccgcccga ctccttgtgt cggctaggtt
     3001 tgactggtgg atatcgcggg caagaaggtg cttctgaccg gtgcgaccgg aggtatcggg
     3061 cgcaggatcg cgagcaccct ggcggcggcc ggggcggagg tgatcgtgtc cggccgttcg
     3121 gccgaggcgc tcaccgagct ggtcgacagc ctgcccggca gcggccactc ggtgctggcg
     3181 gccgacctcg gactcgttgg tgccgcgcag gaacttgcca gccaggccgg agcggttgac
     3241 atgctggtcg ccaatgc
//
LOCUS       DAOQFI010000133         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_14333_length_3250_cov_2.420344, whole genome shotgun sequence.
ACCESSION   DAOQFI010000133 DAOQFI010000000
VERSION     DAOQFI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14333_length_3250_cov_2.420344"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLE93_03300"
     CDS             <1..741
                     /locus_tag="PLE93_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86106.1"
                     /translation="ILRKKLKKNAPRSLRSARSKAAVKYRKCLARMPDVKGDRVHLFK
                     SNAHYYEPAGPVSWDMAMRNTRKDWLVQLKAGDTLEVTTTYETKRASWYENMGIAISY
                     MADGTGGKDPYRTRMDYTGPINHRHYSENNDHGGKLPVVGPDPTGLENGPFASDPFVI
                     SGYSYGQGDYRLPGAAGRPPVVEKGDTFTFSLSDGDVGNKIWHSLTSCAAPCNRSTGI
                     AYPIADGDFRFDSGQMGVGGAPTVNQTT"
     gene            840..1337
                     /locus_tag="PLE93_03305"
     CDS             840..1337
                     /locus_tag="PLE93_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86107.1"
                     /translation="MIAAVPLSEWSDFFVASAGASAALAGLVFVAISINVARILQLDG
                     VPELGLVTLLLLIGVLVVSMFGLIPDQSEHAFGVELLVQSAFWTLVIAFLIRRSAAGL
                     GEGGGRLASRVILPVFGTLPYLAGAVLLITGSDIAMYWVFAGMLGAILAAVMNAWILL
                     VEILR"
     gene            complement(1231..>3250)
                     /locus_tag="PLE93_03310"
     CDS             complement(1231..>3250)
                     /locus_tag="PLE93_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86108.1"
                     /translation="AAGVFVAAVLTLLAVANGARAEILKTEVLPGGVQRTTYRIGPVD
                     VTPGQNRIAYRPIEGASRPAVDGWITRIKPDLVYEDGSIPDSSKVMFHHGVWINLSTN
                     EQFYATGEEKTILDLPEGFGYRYKTDEGWLLNDMIHNLTPQAMKLYFEYTLDFIPDTA
                     AEAAGIVRARPIWMDVQNGSIYPVFDVWRDSGGDDGEFTYPEDATNPYPPGNQKNVKS
                     IPANGMLIATTGHVHTGGLSTDLYLRRDGATYQGPNCPDRISYEDELKPLQQKNDSLV
                     ARINKLKQKNAKMTRTQQKIQRSMKNHQSKSKAKSLIKDIKRRKKSNSKTIAMVNRLR
                     AKNKIQLDKVRAMDKAERERDAACRGTQPSVDDGNRVHLFKSTADYFDPRGPISWDMA
                     MVSTDNDWRVRVKAGDKLELQTTYETKRASWPESMGINIVYWVRDSAYAAAANAPDPY
                     VTNVAQKGVLNHGHLKENEDYGGETGKNGSKLVGPDPTKLPDGPETGGPFTISGFSYN
                     QGDFRLPGSAGRPPVVPQGQSFTFKLSDSDITNEVWHSITSCAAPCNKSTGISYPIPN
                     GEVQFESGQMGPGKAPTVGYTQWSTPSDLKKGTYTFFCRIHPLMRGAFRVKYPDRPAK
                     PRSRSLPGPARPCLSGGSRRAGSRRSSPPPGSLRACRRKPSTSRCRCP"
BASE COUNT          513 a         1019 c         1071 g          647 t
ORIGIN      
        1 atcctcagga agaagctgaa gaagaacgcc ccccgctcgc tgcggagcgc gcggagcaag
       61 gccgcggtga aataccgcaa gtgcctggcc aggatgcccg atgtcaaagg tgaccgggtc
      121 catctcttca agtcgaatgc ccattactac gagccggccg gacctgtttc ctgggacatg
      181 gcgatgcgca acacgcgcaa ggactggctg gtccagttga aggccggcga cacgctggaa
      241 gtcaccacga cctacgaaac gaagcgtgcc tcctggtacg agaacatggg catcgcgatc
      301 tcctacatgg ctgacggcac cggcggcaag gacccgtaca ggacccggat ggactacacc
      361 gggccgatca accacaggca ttactccgag aacaacgacc acggcggcaa gctgccggtg
      421 gtcgggccgg atccgaccgg gctcgagaac gggccgttcg cgagcgaccc cttcgtgatc
      481 agcggctaca gctacggcca gggcgactac cgcttgcccg gcgcggccgg caggccgccg
      541 gtggtggaga agggtgacac cttcaccttc tcgctctcgg acggtgacgt cggcaacaag
      601 atctggcact cgctgacttc ctgcgccgca ccctgcaaca ggtcgacggg gatcgcctat
      661 ccgatcgccg acggggactt ccgcttcgac tccggccaga tgggcgtcgg cggagcgcca
      721 acggtcaacc aaacgaccta aaccacaccg gccaatcttc cggtcggcac acacaccttc
      781 ttctgtcgca tccacccgct gatgcgcggt gcgatccggg tggtgcccaa gggcagcggt
      841 tgatcgccgc cgttccgctt tcggaatgga gcgacttctt cgtcgcctcg gccggggcct
      901 cggcggccct ggccggcctc gttttcgtgg cgatctcgat caacgtcgcg cgaatcctgc
      961 agcttgacgg ggtaccggaa ctcggcctgg tcaccctgct gctcctgatc ggggtgctgg
     1021 tcgtctcgat gttcgggctg attcccgacc agtcggaaca tgccttcggg gttgagctgc
     1081 tggtccagtc ggcattctgg acgctcgtca tcgccttcct gatcaggcga agtgcggccg
     1141 gtctcggtga aggaggcggt cgcctggcct cccgggtcat cctgccggtc ttcgggaccc
     1201 tgccctatct ggccggcgcg gtcctgctga tcacgggcag cgacatcgcg atgtactggg
     1261 ttttcgccgg catgctcgga gcgatcctgg cggcggtgat gaacgcctgg atcctgctcg
     1321 tcgagatcct ccgctaaggc acggacgagc cggccccggg agggaccggc tcctcggctt
     1381 ggcgggtcgg tccgggtact tgaccctgaa ggcgccgcgc atcagcgggt ggatgcggca
     1441 gaagaaggtg taggtgccct tcttcagatc gctgggcgtc gaccattggg tgtagccgac
     1501 cgtcggagct ttgcccggtc ccatctggcc cgactcgaac tggacttcgc cgttcgggat
     1561 cggatacgag atgccggtcg acttgttgca gggtgccgcg caggaggtga tcgagtgcca
     1621 gacctcgttc gtgatgtccg aatcggacag cttgaaggtg aaggactggc cctgcgggac
     1681 gaccggtggg cgaccggctg aacctggcag gcggaagtca ccctggttgt agctgaaacc
     1741 gctgatcgtg aacggcccgc cggtttccgg gccgtcaggc agtttggtcg gatcgggtcc
     1801 aaccagcttg ctgccgttct tgccggtctc gccaccgtag tcctcgttct ccttcaggtg
     1861 gccatgattc agcacaccct tctgggcgac gttggtcaca tacgggtcgg gagcgttggc
     1921 cgcggcggcg taggcggaat cacggaccca gtacacaatg ttgatgccca tcgactcggg
     1981 ccaggaggcc cgcttcgtct cgtaggtcgt ctgcaactcc agcttgtccc cggccttgac
     2041 ccggacccgc cagtcgttgt cggtgctgac catcgccatg tcccaggaga tcggaccgcg
     2101 cgggtcgaaa tagtccgcgg ttgacttgaa caggtggacg cggttgccgt cgtcgaccga
     2161 cggctgggta ccgcggcagg cggcgtcgcg ctcgcgctcg gccttgtcca tggccctgac
     2221 cttgtcgagc tggatcttgt tcttcgcccg gaggcggttg accatcgcga tggtcttcga
     2281 gttggacttc ttgcgccgtt tgatgtcctt gatcaggctc ttcgctttcg acttgctctg
     2341 gtggttcttc atcgagcgct ggatcttctg ctgggtccgc gtcatcttcg cgttcttctg
     2401 cttgagcttg ttgatccgcg ccaccaagct gtcgttcttc tgctgaagcg gcttcagctc
     2461 gtcctcgtag gagatccggt ccgggcagtt cggcccctgg taggtggcac catcgcggcg
     2521 caggtacagg tcggtcgaga gccccccggt gtggacgtgg ccggtggtcg cgatcagcat
     2581 gccgtttgcc gggatcgact tgacgttctt ctggttgccg ggcgggtagg ggttggtcgc
     2641 gtcctcggga taagtgaact cgccatcgtc cccgccgctg tcacgccaga cgtcgaagac
     2701 ggggtagatg ctgccgttct ggacgtccat ccagatcgga cgggcccgca cgatgccggc
     2761 ggcttcagcc gcagtgtccg ggatgaagtc gagcgtgtac tcgaagtaga gcttcatcgc
     2821 ctgcggggtg aggttgtgga tcatgtcgtt cagcaaccag ccctcgtccg tcttgtagcg
     2881 gtaaccgaag ccctcgggca ggtcgaggat ggtcttttct tcgccggtcg cgtagaactg
     2941 ctcgttggtc gagaggttga tccagacccc gtgatggaac atcaccttgc tcgagtcggg
     3001 aatcgagccg tcctcgtaga cgaggtccgg cttgatccgg gtgatccagc cgtcgacggc
     3061 tggccgggag gcaccttcga tcgggcgata ggcaatccgg ttctgacccg gggtcacgtc
     3121 gaccgggccg atccggtagg tggtcctctg aaccccgccg ggcaacacct cggttttgag
     3181 gatctcggcg cgagcgccat tggcgaccgc cagcaaagtc aacaccgctg caacgaaaac
     3241 tcctgccgcg
//
LOCUS       DAOQFI010000134         3247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_14356_length_3247_cov_3.059211, whole genome shotgun sequence.
ACCESSION   DAOQFI010000134 DAOQFI010000000
VERSION     DAOQFI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14356_length_3247_cov_3.059211"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLE93_03315"
     CDS             complement(<1..925)
                     /locus_tag="PLE93_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH86109.1"
                     /translation="MPEAGGGMKRVFVTGGNGFLGHSVVRGLAEAKDQVELVVSGDLG
                     HPTDARRVEGVDYIVTDVTDAASVSGPIGEHGIDTVVHLAAIVNPGNLSTEQEYEVDV
                     TGSKHVFDACLAHDVKRVVVSSSGAAYGYHADNPAWLTEDDPVRGTETFPYSLHKRLV
                     EEQLAALRESNPELEQVVLRIGTILGETVDNQITDLFKAKRPLKIRGSESPFVFIWDQ
                     DVVGVMLQAVLGEKTGIFNVAGDGAMTIDEIAAELGKKPRTVPVGVLKAGLGVAHFFN
                     LTPHGAAQTDFLAYRPVLDNRRLKVEFGYTPK"
     gene            complement(918..1901)
                     /locus_tag="PLE93_03320"
     CDS             complement(918..1901)
                     /locus_tag="PLE93_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNH86110.1"
                     /translation="MSLAFLSSLPGLPFGAEDIDSVTLNTSDGMLIVLSVIIGVIMFG
                     IALEMKVDDFRHVMKLPKAMAVGVAAQFICLPAITYLLTVALNFRPSIAMGMILVACC
                     PPGNISNILVYRARGNVALSLSMTALSNFLAIFLMPLNIAFWGGMHPEASQVLKDVDV
                     SAIELLLDIVVLIGIPLTLGLLISSHRPGLAKKVQPIVKKFSLIFLILFIVAALAGNF
                     KVFVDHIALVAVAVFLHDTLALLLGYGIAAGFKVSEPNRRAITFEVGVRNAALGLGIA
                     ISVFDGLGGVAMVAAWWGVWDLIAGLTLSSWWARRPTGDIEESTLEAAESA"
     gene            complement(1898..>3247)
                     /locus_tag="PLE93_03325"
     CDS             complement(1898..>3247)
                     /locus_tag="PLE93_03325"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH86111.1"
                     /translation="IGFAIVAPEGDSQEKLPWALVGAGPTGLSAARNLSRRGIPFVGF
                     EIHTDVGGLWDIESEKSTMYESAHLISSKTTTAYDEFPMRDEVADFPSHWEMNSYFKD
                     YADTFDLRRNFRFGTEVTAAEPEDPESESSVWKVTIRNEDGEEESGRYAGVIIANGIL
                     SEPSIPTFEGEEEFEGEVLHTASYKRPDIFRDRKVLIVGCGNSGCDIAVDAVHYAKSI
                     EMSLRSGYWFFPKYIFGRPSDTLNEGKALPAFLKTRIDGVFIKFLTGDPARLGFPEPE
                     GRIYETHPVMNTQVLYYAGHGDIHIRKNIKRFTKTGVEFVDGSTGDYDMVMLATGYKL
                     HYPFIDPQYLNWKGNSPDLYLNIFTPKFRNIFVLGMVEATGLGWQGRYNQAHLVAEFI
                     DAGQRNPVRADEMWRRIQGPKPDLFAGYNYRQLDRLPFYVNKDAYRAQIHEHLEIFE"
BASE COUNT          591 a         1012 c         1053 g          591 t
ORIGIN      
        1 acttcggggt gtagccgaac tcaaccttca ggcggcggtt gtcgaggacc gggcggtagg
       61 cgaggaagtc ggtctgggcc gcgccgtgcg gggtcaggtt gaagaagtgg gcgacgccga
      121 gaccggcctt caggaccccg accggaaccg tgcggggctt cttgccgagc tcggcggcga
      181 tctcgtcgat cgtcatggcg ccgtcacccg cgacgttgaa gatgccggtc ttctcgccca
      241 gcaccgcctg caacatcacc ccgaccacgt cctggtccca gatgaagacg aacggtgact
      301 cggagccgcg gatcttcagc ggtcgtttcg ccttgaagag gtcggtgatc tggttgtcca
      361 ccgtctcgcc aaggatcgtg ccgatccgga gcaccacttg ctcgagctcc ggattggatt
      421 cccgaagcgc cgccagctgc tcctcgacca gacgcttgtg caggctgtag gggaaggttt
      481 cggtgccgcg gaccggatcg tcctcggtca gccaggccgg gttgtcggcg tggtagccgt
      541 aggccgcgcc gctggaagag accaccacac gcttcacatc gtgagcgagg catgcgtcaa
      601 acacatgctt cgacccggtc acatcgacct catattcctg ttcggtcgag aggttgccgg
      661 gattgacgat cgccgcgagg tggacgaccg tgtcgatccc gtgctcgccg atcggcccgg
      721 aaaccgaggc ggcatcggtc acgtcggtga cgatgtaatc gaccccttcg acccgccgcg
      781 cgtcggtcgg gtggccgaga tcacccgaga cgacgagctc gacctggtcc ttcgcctcag
      841 ccaggccccg caccaccgaa tggccaagga aaccgttgcc gccggtgacg aatacgcgct
      901 tcatcccgcc gccggcttca ggcactttcg gccgcctcca gcgtgctctc ttctatgtcc
      961 ccggtcggtc tcctcgccca ccagctggag agggtcaggc cggcgatcag gtcccagacc
     1021 ccccaccagg cggccaccat cgccacgcct ccgagaccgt cgaagacgct gatcgcgatg
     1081 ccaagaccga gcgccgcatt gcgcaccccg acctcgaatg tgatcgctct tcggttcggc
     1141 tctgaaacct tgaagccggc cgcgatcccg tacccgagaa gcagcgcgag cgtgtcgtga
     1201 aggaagacgg cgaccgcgac cagggcgatg tggtcgacga agaccttgaa gttgccggcc
     1261 agggccgcga cgatgaaaag gatcaggaag atcagactga acttcttgac gatcggctgg
     1321 accttcttgg ccaggcccgg gcggtggctg gagatcagca ggccgagcgt cagcggaatt
     1381 ccgatcagca cgacgatgtc gagcagcagt tcgatcgcgc tgacgtcaac gtccttgagg
     1441 acctgggatg cctccggatg catgcctccc cagaaggcga tgttgagcgg catcaggaag
     1501 atcgcgagga agttcgaaag ggcggtcatc gaaagcgaca gggccacgtt tcctctggcc
     1561 cggtacacga ggatgttcga gatgtttccc ggcggacaac aggcgaccag gatcatcccc
     1621 atggcgatcg agggccggaa gttgagggcc accgtgagca ggtaggtgat cgccggcagg
     1681 cagatgaact gggccgccac gccgaccgcc atcgccttcg gaagcttcat cacatgacgg
     1741 aagtcatcga ccttcatctc cagggcgatg ccgaacatga tcaccccgat gatcacgctg
     1801 agcacgatca gcatgccgtc gctggtgttc agggtcacgc tgtcgatgtc ctccgccccg
     1861 aaaggcaggc ccggcagaga ggaaaggaag gccaggctca ttcgaagatc tccaggtgct
     1921 cgtggatctg ggcccggtag gcgtccttgt tcacatagaa cggaagccga tcgagctggc
     1981 gatagttgta gccggcgaag aggtccggct tcgggccctg gatccgacgc cacatctcgt
     2041 cggcccggac cgggttccgc tggccggcgt caatgaactc ggcgaccagg tgggcctggt
     2101 tgtagcgacc ctgccagcca agaccggtcg cctcgaccat cccgagcacg aagatgttgc
     2161 ggaacttcgg ggtgaagatg ttcaggtaga ggtcgggact gttgcccttc cagttcaggt
     2221 actgcgggtc gatgaacggg tagtggagct tgtagccggt ggccagcatg accatgtcgt
     2281 agtcaccggt tgaaccgtcc acgaactcga ctccggtctt cgtgaagcgc ttgatgttct
     2341 tgcggatgtg gatgtcgccg tggccggcgt agtagagcac ctgggtgttc atcaccgggt
     2401 gggtctcgta gatccgcccc tccggctcgg ggaagccgag ccgggccggg tcaccggtca
     2461 ggaacttgat gaagaccccg tcgattcggg tcttgagaaa ggccggcagg gccttgccct
     2521 cgttcagggt gtcggagggc cggccgaaga tgtacttggg gaagaaccag tagccggagc
     2581 gaagactcat ctcgatcgac ttcgcgtaat ggaccgcgtc aacggcgatg tcgcagcccg
     2641 agttgccgca gcccacgatc agcaccttcc tgtcgcggaa gatgtcgggg cgcttgtagg
     2701 aggcggtgtg gagcacctcg ccctcgaact cctcctcgcc ctcgaaggtc gggatgctgg
     2761 gctcggagag gatgccgttg gcgatgatca ccccggcgta gcgtccgctc tcctcctccc
     2821 cgtcctcgtt tcggatcgtc accttccaga ccgacgattc cgactccgga tcctcgggct
     2881 cggccgcggt cacctcggtg ccgaaacgga agttccttcg caggtcgaag gtgtctgcgt
     2941 aatccttgaa gtaactgttc atttcccaat ggctggggaa atcggcgacc tcgtcgcgca
     3001 tcgggaattc gtcgtaggcg gtggtcgtct tcgaggagat caggtgggcc gactcataca
     3061 tcgttgactt ctccgactcg atgtcccaga gcccgccgac atcggtgtgg atctcgaatc
     3121 cgacgaaggg aataccgcgg cgggagaggt tccgtgccgc cgagagtccg gtcgggccgg
     3181 ccccgaccaa tgcccacggc agtttctctt gtgaatcccc ctcaggcgcc acgatcgcga
     3241 atcctat
//
LOCUS       DAOQFI010000135         3228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_14495_length_3228_cov_4.007879, whole genome shotgun sequence.
ACCESSION   DAOQFI010000135 DAOQFI010000000
VERSION     DAOQFI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14495_length_3228_cov_4.007879"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PLE93_03330"
     CDS             complement(<1..1534)
                     /locus_tag="PLE93_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNH86112.1"
                     /translation="MAHDYDYVIVGSGFGGSVSALRLAEKGYKVAVIETGRRFEDKDF
                     AKTAWNMRRYFWLPKFGLRGILRMSMFKDVFVVSGCGVGGGSLGYANTLYRPRPAFYT
                     DPQWDGLADWEAELGPHYVTAERMLGVTLYRDTGPADALLHEYADEIGADKTYSNTQV
                     GVFFGEAEKTVPDPYFGGEGPERTGCSKCGSCMVGCRHGAKNTLVKNYLFFAEKLGVK
                     VFPGTEVTSVRPIGGPDGEHRDGSAGYELRTDRSGAVLRHRRRKLTAAKVIFSAGALG
                     TNKLLANCLHNGDLPNLSKRVGHVVRTNSESIQAVTAHDDERDFSDSVAITSSIYPDP
                     DTHIEVVTYGQAGDVMSRLYTIYTGNGTRLTRPIRFLATMARHPIDTLHLLFMPRHWS
                     RRTVILLVMQALDSAMRLKPVPKKFGKGVRLQTEQDPERPNPTFIQAAEDATKWFAKR
                     TGGIPQVSFGESLFNIPTTAHILGGAVVGHSPEDGVIDKDNHVFGYENLMVCDGSAVP
                     ANP"
     gene            1768..>3228
                     /locus_tag="PLE93_03335"
     CDS             1768..>3228
                     /locus_tag="PLE93_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86113.1"
                     /translation="MKQRLAVIVGILAAFLVGAFAVTAPASAADAGAVKLQKQVKAAQ
                     KKATATCKKVKKAKKGKSKAKAKKRCKSAKQNVTRLQKKLKAYNAAQYFDVCKHGCKY
                     RTVQKGADAAGLWQKKSKRKATVRVKPGTYVEGVFLDGRNPKYDYDGLTIMGTSKNAK
                     DVILEGENAKTIVDGVPQVANNAIEGRSIIGLVMKNMWARHYQNNTFFVWAGHDGTER
                     CADYTMDNLVSSDTRSYGFFARNCFGGVIENSSGYHHGDSAVYIGETPCDSPDWTNRT
                     VAAERTPCQADPDWTIIRNVKSYANVLGYSGTNSKYVDIKNSAFYNNGAGIVPNTLDS
                     EQFEPSGWLKIHNNDIFWNNYNYYSTGSDFQTVATIGDVNYPIGIGVVLYGTDSVEVY
                     DNNIFGNEKWGAATFSGPELFGVNVGDDAMNLNNKFIDNNMGRNGDDPNGKFDFFSDY
                     SGGGNCWQGNTAGSSFAPGNGTVALSTIYPSSCPQPV"
BASE COUNT          635 a         1017 c          996 g          580 t
ORIGIN      
        1 ccggattggc cggcacggcc gagccgtcac agaccatcag gttctcgtag ccgaagacgt
       61 ggttgtcctt gtcgatcacc ccgtcttcgg ggctgtggcc gaccacggca ccgccgagga
      121 tgtgggcggt ggtcgggatg ttgaagagcg actcgccaaa ggaaacctgg gggatgccgc
      181 cggttcgctt cgcgaaccac ttggtcgcgt cctcggcggc ctggatgaag gtcgggttgg
      241 gccgttccgg gtcctgctcg gtctggagcc ggactccctt gccgaacttc ttcgggaccg
      301 gcttgagccg catcgccgag tcgagcgcct gcatgaccag gaggatcacg gttcgccttg
      361 accagtgccg gggcatgaac agcaggtgaa gggtgtcgat cgggtggcgt gccatcgtgg
      421 ccaggaacct gatcggccgg gtcaaccggg tcccgttgcc ggtgtagatc gtgtagaggc
      481 ggctcatcac atccccggcc tggccgtagg tgacgacctc gatgtgagtg tcggggtcgg
      541 ggtagatgct ggaggtgatt gccaccgaat cactgaagtc tctttcgtca tcgtgcgcgg
      601 tcacggcctg gatcgactcc gagttggtcc gaaccacatg gccgaccctc ttcgagaggt
      661 tcggcaggtc gccgttatgg aggcagttgg cgagcagttt gttggtgccg agggctccgg
      721 ccgaaaagat caccttggcg gccgtcagtt tccggcgccg atgccgcagc acggcaccag
      781 agcggtcggt tctcagctcg tagcccgcgg agccgtcccg atgctcgccg tcgggcccgc
      841 cgatcggcct gaccgaagtg acctcggtgc cggggaacac cttgaccccc agcttctcgg
      901 cgaagaagag gtagttcttg accagggtgt tctttgctcc gtgccggcaa ccgaccatgc
      961 atgaaccgca cttgctgcag ccggtgcgct ccgggccctc gccgccgaag tagggatcgg
     1021 ggacggtctt ctcggcttca ccgaagaaga ccccgacctg ggtgttcgag taggtcttgt
     1081 cggcgccgat ctcgtcggcg tattcgtgaa gcagcgcgtc ggccggaccg gtgtcgcggt
     1141 aaagcgtcac gcccagcatc cgctcggcgg tcacgtaatg ggggccgagc tcggcttccc
     1201 agtcagccag gccatcccac tggggatcgg tgtagaaggc aggccggggc cggtagaggg
     1261 tgttggcgta accgaggctg ccgcctccca cgccgcaacc ggaaacgacg aagacgtcct
     1321 tgaacatcga catcctcagg atcccgcgca ggccgaactt cggcagccag aagtaacgcc
     1381 gcatgttcca ggccgtcttc gcgaagtcct tgtcttcgaa gcggcggccg gtctcgatca
     1441 cggccacctt gtagcccttt tcggccagtc tcagggcgga aacactgccg ccgaatcccg
     1501 aaccaacgat cacgtagtcg tagtcgtgcg ccatgacaat caagtgtaac cgacatgaag
     1561 ccttgtcaat ttctcgcatt gcagtgcgga ttgctccgcc ccgggagtcg gacaggaagc
     1621 ggaaatcgcg tcagagcgcc aattcatccg atccgggtta agcgcaggac cgcaaccttt
     1681 ccgttaaggg tggtttagag tgtccggatg gcttttccgc attcctgcgg tcgagccgca
     1741 cacacttttc acacttcaag gagagagatg aagcaacgtt tggctgtaat cgtcggcatt
     1801 cttgccgcgt tcctggtggg cgctttcgcc gtcaccgctc cagcttcggc ggctgacgcc
     1861 ggtgctgtga agctgcagaa gcaggtcaag gccgcgcaga agaaggccac ggctacctgc
     1921 aagaaggtca agaaggcaaa gaagggcaag tcgaaggcca aggccaagaa gcgctgcaag
     1981 tccgccaagc agaacgtgac tcgtctccag aagaagctga aggcctacaa cgccgcccag
     2041 tacttcgacg tctgcaagca cggctgcaag taccgcaccg tccagaaggg cgctgacgcg
     2101 gccggcctgt ggcagaagaa gtccaagcgc aaggcgaccg tgcgagtcaa gcccggcacc
     2161 tacgttgagg gcgtcttcct cgatggtcgt aaccccaagt acgactacga cggcctgacc
     2221 atcatgggca cctcgaagaa tgcgaaggac gtcatcctcg agggcgagaa cgccaagacg
     2281 atcgtcgacg gtgttcccca ggtcgccaac aacgcgatcg aaggccgcag catcatcggc
     2341 ctcgtcatga agaacatgtg ggcccgccac taccagaaca acacgttctt cgtctgggcc
     2401 ggtcacgacg gcaccgagcg ttgtgccgac tacaccatgg acaacctcgt ctcctccgac
     2461 acccgttcct acggcttctt cgcccggaac tgcttcggtg gtgtgatcga gaactcctcg
     2521 ggctaccacc acggtgactc cgccgtctac atcggtgaga ccccgtgtga ttcgcccgac
     2581 tggaccaacc ggaccgttgc cgcagagcgc acgccctgcc aggccgatcc ggactggacc
     2641 atcatccgga atgtcaagag ctacgccaac gtcctcggct actcgggcac caactccaag
     2701 tacgtcgaca tcaagaactc ggcgttctac aacaacggtg ccggcatcgt gccgaacacc
     2761 ctcgactccg agcagttcga gccgagcggc tggctgaaga tccacaacaa cgacattttc
     2821 tggaacaact acaactacta ctcgaccggg tctgacttcc agacggttgc cacgatcggt
     2881 gacgtcaact acccgatcgg catcggcgtc gtcctgtacg gcaccgacag cgtcgaggtc
     2941 tacgacaaca acatcttcgg caacgagaag tggggcgcag ccaccttctc cggaccggaa
     3001 ctgttcggtg tcaacgtcgg cgacgacgcg atgaacctga acaacaagtt catcgacaac
     3061 aacatgggcc gcaacggcga cgatccgaac gggaagttcg acttcttctc ggattactcg
     3121 ggcggtggca actgctggca gggcaacact gccggttcca gcttcgcccc cggcaacggt
     3181 acggtcgcgc tttccacgat ctacccgagc agctgcccgc agccggtg
//
LOCUS       DAOQFI010000136         3191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_14810_length_3191_cov_3.517219, whole genome shotgun sequence.
ACCESSION   DAOQFI010000136 DAOQFI010000000
VERSION     DAOQFI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14810_length_3191_cov_3.517219"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLE93_03340"
     CDS             <1..581
                     /locus_tag="PLE93_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86114.1"
                     /translation="FSACNATVEDGVRLGADAIGYTLYVGSPRQDEDLAQLRQVRQDC
                     DRFGMPLVVWSYPRGEAIDAKGGAGSFYAIDYAARMAMEMGADVVKLNYPKIDPEKDK
                     DAQPPYNEMDVTAEEAMRQCVESAGRSLVVLSGGSKTDDETVLGHCKSVMNAGGSGVI
                     FGRNVWQREWSEALEIIAQIKETLLANVKRTP"
     gene            590..1177
                     /locus_tag="PLE93_03345"
     CDS             590..1177
                     /locus_tag="PLE93_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNH86115.1"
                     /translation="MKQGNIPTPPGQFGNLFPDHSRGYLILFVVILAAFGYWLLNRDD
                     GNKDPGLQGSGSSVLRVDVDRVVDGDTALVWMDGHQQYVRYIGIDTPESVKEGYPIEC
                     YGPEAKQFNEELLDRRSTVKLVFDDELRDRYGRLLAYVYSGRTLLQAELLEGGYATTI
                     EVPPNTSRAGEFSAMESEARDAGRGGWSACPGFGR"
     gene            1332..1565
                     /locus_tag="PLE93_03350"
     CDS             1332..1565
                     /locus_tag="PLE93_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86116.1"
                     /translation="MRQSANQFEAAFEQQKILEQRRREQLRNRAVNRSRARRVSRSQQ
                     QGKVRFSVLAVCLTVTVVVVTIVMFEALLFFMS"
     gene            complement(1608..1790)
                     /gene="csrA"
                     /locus_tag="PLE93_03355"
     CDS             complement(1608..1790)
                     /gene="csrA"
                     /locus_tag="PLE93_03355"
                     /inference="COORDINATES: protein motif:HMM:NF002469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNH86117.1"
                     /translation="MLVLTRKKEQSIIIGENVEIMVLGVSGDKVRLGITAPREVDVFR
                     KEVIDERGSKAETENA"
     gene            2017..2541
                     /locus_tag="PLE93_03360"
     CDS             2017..2541
                     /locus_tag="PLE93_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86118.1"
                     /translation="MSASELREFRRQHDLSPDDDADGTTEVDLRDEIEAEIEAAAAEE
                     EAARKKAEEEASAAEAAKAAEREARGETDGEDDDDAEGESDSGEETETDGSEAEGRPR
                     RRRRGRRGRRGRGGSNKLEGTFDHGDEGYGLWLDPSIQDVPAYAENWAGKRDVVITVE
                     ADQIIIRRAGEEQE"
     gene            complement(2623..2949)
                     /locus_tag="PLE93_03365"
     CDS             complement(2623..2949)
                     /locus_tag="PLE93_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86119.1"
                     /translation="MAYPLENALFNWEAGIDRLRELERSGELRGDDVTVPVREELRRR
                     LGATFTATDLADLYGEGTDWALLLPGVDPGLSDVQELIDAAFWLHLQAAGDFAGGRVV
                     VDLELD"
     gene            complement(2985..>3191)
                     /locus_tag="PLE93_03370"
     CDS             complement(2985..>3191)
                     /locus_tag="PLE93_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86120.1"
                     /translation="FNEYSQPISSPETGWAENDPALEIVEPRTKPPEAEKPVIVWRVT
                     GPMDPALCSRLGPESNFIPGLKGE"
BASE COUNT          604 a         1008 c         1028 g          551 t
ORIGIN      
        1 ccttctccgc ctgcaacgcg accgtcgagg acggcgtccg gctcggcgcc gacgcgatcg
       61 gctacacgct ctacgtcggc tcgccgcggc aggacgagga cctcgcccag ctgcgccagg
      121 tccgccagga ctgtgaccgc ttcggcatgc cgctggtcgt ctggtcctac ccgcgcggtg
      181 aggcgatcga cgccaagggc ggcgccggtt ccttctacgc gatcgattac gcggcccgca
      241 tggcgatgga gatgggcgcc gacgtggtca agctcaacta cccgaagatc gacccggaga
      301 aggacaagga cgcgcagcct ccgtacaacg agatggacgt gaccgccgaa gaggcgatgc
      361 gccagtgcgt cgagtcggcc ggccgttcgc tggtcgtgct ctccggcgga tccaagaccg
      421 acgacgagac cgtgctcggt cactgcaagt cggtgatgaa tgccggcggt tccggcgtga
      481 tcttcggccg caacgtctgg cagcgcgaat ggagcgaggc cctggagatc atcgcccaga
      541 tcaaggagac gctgctcgcc aacgtcaagc gcactccgta aggggtcgtt tgaaacaggg
      601 gaacattccg acaccccctg gccagttcgg gaacctcttc ccggaccaca gccgggggta
      661 tctgattctc ttcgtcgtca tcctcgcggc cttcggttac tggctgctga accgggacga
      721 cggcaacaag gacccggggc ttcaaggctc cgggtcttct gttctcaggg tcgatgtcga
      781 ccgggtggtg gatggcgaca ctgccctggt ctggatggac ggccaccagc aatacgttcg
      841 ctacatcggg atcgacaccc cggaatcagt caaggagggt tacccgatcg agtgctacgg
      901 gccagaggcc aagcagttca acgaggagct gcttgaccgg cgttcgaccg tgaagctggt
      961 cttcgacgat gagctgaggg atcggtatgg ccggctgctg gcctatgtct actcgggccg
     1021 cacgctgctg caggccgagc tcctggaggg cggctacgcg accacgatcg aagttccgcc
     1081 caacacctcc cgggccggag aattttcggc gatggaaagc gaagcccgcg acgccggccg
     1141 cggtggctgg agcgcctgcc cgggcttcgg gcggtagaac ggccgccgtt tcggccgcct
     1201 ggtctgtgag gaatctgtga cctcagtcac agaaatgtga acgaagactt gagatcggcc
     1261 aatcatttgc tacggtggac ggacggcctg tttctgaaag ttcgatttca cccggccgaa
     1321 ccactatgag aatgcgtcaa agcgcaaatc agttcgaagc agccttcgaa cagcagaaga
     1381 tcctcgagca gcgccggcgg gagcaactcc gcaaccgggc tgtgaatcgt tcgcgtgccc
     1441 gcagggtgtc gcgttcccag cagcagggca aggtgcgctt cagcgtgctc gccgtctgcc
     1501 tcaccgtgac cgtggtcgtc gtgaccatcg tcatgttcga ggcgctgctc ttcttcatgt
     1561 cctaggaaaa gccgagggct ccccaaagcc cccgggttcc tgtctgttca ggcgttttcg
     1621 gtttccgcct tggagccgcg ctcgtcgatg acttccttgc ggaaaacgtc gacttcccgc
     1681 ggcgcggtga tgcccagacg caccttgtca ccggaaacgc cgagaaccat gatctccacg
     1741 ttttcaccga tgatgatgct ctgttccttt tttctggtca gtaccagcat gtgactccat
     1801 tcgactccga gtggtcggtt cgatacctaa taataggtat tgagacagac gggccacttc
     1861 gtttcttcaa cccctacccg gagaacgcac ttccaagccg tttcaataga ctcggcaacc
     1921 tatatggctc gccgatcact acgcccccaa ctcaaccaga ttagggcctg ggtccgtcag
     1981 ggaaggaccg acgcctggat cgcccaccag cttgaagtga gtgcttccga actccgtgag
     2041 ttccgccgcc agcacgacct ttcgcccgac gatgacgccg acggtacgac cgaggtagat
     2101 ctccgcgacg agatcgaagc cgagatcgaa gccgcggccg ccgaggaaga agcggcccgc
     2161 aagaaggccg aagaggaagc ctcggccgct gaagctgcca aagcagccga gcgcgaggcc
     2221 cgtggtgaga ccgacggcga agacgacgat gatgccgaag gagagtctga ttccggcgag
     2281 gaaacggaaa ccgacggctc cgaagccgaa ggccgacccc gccgccgccg tcgcggccgt
     2341 cgcggccgtc gcgggcgtgg aggcagcaac aagcttgaag gcacctttga ccatggcgac
     2401 gagggatacg ggctctggct cgacccgtcg atccaggatg tcccggccta cgcggaaaac
     2461 tgggccggca agcgcgacgt ggtcataacg gtcgaagccg accagatcat cattcgccgc
     2521 gccggcgaag agcaggaata agcaaaaggt cggctttgcc gaccaaggga aacccgccct
     2581 cccatcaaat tcactgatgg ttgggagggc tccgctcggc ggctaatcga gttctaggtc
     2641 tactactacc cggccgccgg cgaagtctcc ggcggcctga aggtgaagcc agaaggcggc
     2701 gtcgatcagc tcctggacgt ccgaaagccc gggatcgacg ccgggcagga gcagcgccca
     2761 gtcggtgccc tcgccgtaca gatcggccag gtcggtggcg gtgaaggtgg cgccgagccg
     2821 ccgcctgagt tcctcccgga ccggaacggt gacgtcatca ccgcgcagct ctccggaacg
     2881 ctcgagctcg cgtaaccggt cgattccggc ttcccagttg aagagggcgt tctcgagggg
     2941 ataggccacg cggggatgat ggcaggccgg ccgcttcggc cggttcactc gcccttgagc
     3001 ccggggatga agttgctctc cggcccgagc cgggaacaga gcgccgggtc catcgggccg
     3061 gtcacccgcc agacgatcac cggcttttcc gcctcgggag gcttcgttcg cggttcgacg
     3121 atttcgaggg cgggatcgtt ttcagcccag cccgtctccg gagaggagat cggctggctg
     3181 tactcgttga a
//
LOCUS       DAOQFI010000137         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_14960_length_3172_cov_3.539942, whole genome shotgun sequence.
ACCESSION   DAOQFI010000137 DAOQFI010000000
VERSION     DAOQFI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14960_length_3172_cov_3.539942"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..439)
                     /gene="mraZ"
                     /locus_tag="PLE93_03375"
     CDS             complement(2..439)
                     /gene="mraZ"
                     /locus_tag="PLE93_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNH86121.1"
                     /translation="MAFRGQHEHSLDSKDRLTVPRKFRAALDEGYVLAKGPDACLWLY
                     STKDFDAMEERYVAPHSPFGADARNLRRMINASADEGNLDAAGRIRIPDNLKELAGIE
                     GACTVVGAGDYIEIWNEGTWAKLSQDLLSQFGDVAENLAGSTE"
     gene            652..1542
                     /locus_tag="PLE93_03380"
     CDS             652..1542
                     /locus_tag="PLE93_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86122.1"
                     /translation="MTEISDANQLRGAKFKELMSKAKTWIIIVVCSVIAAAAGMIVGP
                     AIAGGAFVLVFLIGIVITFMIADSRAATAFYEAYAQARGLTHQSGGILGGSTPLLRKG
                     DRTRVDQQFNGMLSEGIEGTLALYTYTVTSTDSKGNETSTDYPFTVIMVNMPGTVQHL
                     TDLRVQRQSGLKLFEKFEDAFRGDFGRVTLESEAMRDRYEIFVRKEQDPVWVRRLFSP
                     SFIVWLTDSPPKKFAFELENGWLCAYVPKHRDDTEGFDEMIRLGTHVAGRLEEEVGQS
                     SPAEAHHDSPEVKIEVEEEK"
     gene            1539..2453
                     /locus_tag="PLE93_03385"
     CDS             1539..2453
                     /locus_tag="PLE93_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86123.1"
                     /translation="MSEEAKPGGTFEGASAEGEVAVTHANQLRSAKFREMTRKKSTWA
                     WIIGLSVLAGLIFAGLGFPAMGGAAFVIVFLGSLLVVFMIADSRAEDAFYDYYCETHG
                     LTRSTSPEIGELTPLLRMGDKSNTDEMFSGELSPGMDGNLVLYTYTEVHRDSDGDETE
                     TDYPFTIIHVEMPEIVQHMTDLQVHRAGFKLLDGIQDKFLGNMQRVTLESEAFAKRFE
                     VFVNDSQDPIWTRRLFSPSFIVWLTESPPDSFAFELENGHLVAYVSRHMDSTESLENV
                     TRVGTYVANRLLQEVAESSPKAARETGS"
     gene            2450..>3172
                     /locus_tag="PLE93_03390"
     CDS             2450..>3172
                     /locus_tag="PLE93_03390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNH86124.1"
                     /translation="MNPAKGVRPQAILALASVASVALNMWIGRGITFSTDELVWFMLS
                     PDLDLSGAFHPYVGHLILTSKALYKLIFETIGPDYRVIRLAAAMVLVGVVWILYRLLR
                     RYCGEWPALAISLVVLFFSGDPAHVFHGNGITVLGSVGLGLFALLMLSEPATRRSVLA
                     CLALCLGVATYSQTLPFLVGTTVFLIASKRIRDLWVPAIPFLGYFAWWIWARDLPGSS
                     ESSLEPERIFMLPVWGFRATGAV"
BASE COUNT          551 a         1055 c          965 g          601 t
ORIGIN      
        1 gtcactccgt gctcccggcg aggttctcgg ccacgtcgcc gaactggctg agcaggtcct
       61 ggctcagttt ggcccaggtc ccttcgttcc agatctcgat gtagtcaccg gcgccgacca
      121 cggtgcaggc gccctcgatt ccggccagct ccttgaggtt gtccggaatg cggatgcggc
      181 cggccgcatc gagattgccc tcgtctgcgc tggcgttgat catccggcga aggttgcgcg
      241 cgtcggcccc gaacgggctg tggggagcga cgtagcgctc ttccatcgcg tcgaagtctt
      301 tggtcgagta gagccagagg caggcatccg ggcctttggc gagaacgtat ccctcgtcga
      361 gagcggcacg gaacttgcgg ggcaccgtga ggcggtcctt ggaatcgagg ctgtgttcgt
      421 gctggcctct gaacgccacc gctcctcgtc ctttctttgc ctcgccgtgg gttgaccggg
      481 aaagagttgg gccggggtgg tgagagcggg aggagctcca tcaccccggc ccttggcttc
      541 aactcgatgg caatgtaacc cactttgccc cactttgcaa cacaaccccc cactttcttc
      601 caaaagttgc aacggcgatt gcaccgcccg gtcatgaaat aacctcccga tatgaccgag
      661 atctccgatg cgaatcagct gcgcggcgcc aagttcaagg agttgatgag caaggcgaag
      721 acctggatca tcatcgtcgt ctgcagcgtg atcgccgctg ccgccgggat gatcgtcggc
      781 ccggcgatcg ccggcggcgc cttcgttctc gtcttcctga tcgggatcgt gatcactttc
      841 atgatcgccg acagccgggc tgccaccgcg ttctacgagg cctacgccca ggcacgcgga
      901 ctgacccatc agtccggcgg catcctcggc ggctccaccc cgcttctgcg gaaaggtgac
      961 cggacccggg tcgaccagca gttcaacggc atgctctccg aggggatcga gggaaccctc
     1021 gccctctaca cctacacggt taccagcacc gactcgaagg gcaacgagac atcgaccgac
     1081 taccccttca ccgtgatcat ggtcaacatg ccgggcaccg ttcagcacct caccgacctc
     1141 agggtgcaga ggcaatcggg tctgaagctg ttcgagaagt tcgaggacgc cttccgcggc
     1201 gacttcggcc gggtcaccct ggaaagcgag gcgatgcggg accgctacga gatcttcgtc
     1261 aggaaggaac aggatccggt ctgggtccgg cgtctgttct cgcccagctt catcgtctgg
     1321 ctgaccgaca gcccgccgaa gaagttcgcc ttcgaactgg agaacggctg gctctgcgcc
     1381 tatgtgccca agcatcgcga tgacaccgaa ggcttcgacg agatgatcag gctcggtacc
     1441 catgtggcgg gccgcctcga agaggaagtc ggccagagct cacctgccga agcgcatcac
     1501 gactcgcccg aagtcaagat cgaagtggaa gaagagaagt gagcgaggaa gcgaagcctg
     1561 gcggcacctt tgaaggtgcg agcgccgaag gcgaggtggc ggtcacccac gccaaccagc
     1621 tccgctcggc caagttcagg gagatgaccc ggaagaagtc gacctgggcc tggatcatcg
     1681 ggctttcggt cctcgccgga ctgatcttcg ccgggctcgg cttcccggcg atggggggcg
     1741 ccgccttcgt catcgtcttc ctcggctccc tgctggtggt cttcatgatc gccgacagcc
     1801 gggccgaaga cgccttctac gattactact gcgaaacgca cggcctgacc cggagcacct
     1861 cgcccgagat cggcgaactg accccgctgc tccgcatggg tgacaagtca aacacggacg
     1921 agatgttctc cggcgagctt tcgcccggga tggacggcaa cctcgtgctc tacacctaca
     1981 ccgaggtcca tcgcgattcg gacggtgacg aaacggaaac cgactacccc ttcacgatca
     2041 tccacgtcga gatgccggag atcgtccagc acatgaccga ccttcaggtc caccgggcgg
     2101 gcttcaagct cctcgacggg atccaggaca agttcctcgg caacatgcag cgggtgaccc
     2161 tcgaaagcga agcctttgcc aagcggttcg aggtgttcgt caacgacagc caggacccga
     2221 tctggacccg gcgcctcttc tcccccagct tcatcgtctg gctgaccgaa agcccgcccg
     2281 acagtttcgc cttcgaactc gagaacggcc atctggtcgc ctacgtctcg aggcacatgg
     2341 acagcacgga aagcttggag aacgtgaccc gggtcggcac ctacgtggcc aaccggttgc
     2401 tccaggaagt cgccgagtcc tcgccgaagg ccgcgcgcga aaccgggtcg tgaacccggc
     2461 aaagggggtc cggccccagg ccatcctggc gctggcctcg gtggccagcg tcgccctcaa
     2521 catgtggatc ggccggggga tcaccttcag caccgacgag ctggtctggt tcatgctcag
     2581 tcccgatctc gacctttccg gcgccttcca cccctacgtc ggccacctga tcctcacctc
     2641 gaaagccctc tacaaactga tcttcgagac gatcgggccg gactaccggg tgatccgcct
     2701 ggccgcggcc atggttctgg tcggggtcgt ctggattctt taccggctgc tccgccgcta
     2761 ctgcggcgag tggccggccc tcgcgatcag cctggtcgtc ctttttttca gcggcgaccc
     2821 tgcccatgtc ttccacggca acggaatcac cgtgctcggt tctgtcggct tgggcctctt
     2881 cgccctgctg atgctgagcg aaccggcgac ccggcggtcg gtgctggcct gcctcgccct
     2941 ttgtctaggt gtcgccacct attcgcagac gctgcccttc ctggtcggaa ccaccgtttt
     3001 cctgattgcc tcgaaacgga tccgggacct ttgggttccg gcgatcccgt tcctcggata
     3061 tttcgcctgg tggatctggg cccgggactt gcccggttcc tcggagagct cactggaacc
     3121 ggaacggatc ttcatgctgc cggtctgggg cttccgggcc accggcgccg ta
//
LOCUS       DAOQFI010000138         3163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15030_length_3163_cov_3.320785, whole genome shotgun sequence.
ACCESSION   DAOQFI010000138 DAOQFI010000000
VERSION     DAOQFI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_3163_cov_3.320785"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1123)
                     /gene="dnaN"
                     /locus_tag="PLE93_03395"
     CDS             complement(11..1123)
                     /gene="dnaN"
                     /locus_tag="PLE93_03395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNH86125.1"
                     /translation="MKFSIKNNELSGKLALVARTLATGGVNPALGGILVEATDGRLSF
                     SSTDSNLSLRVPVHVEGSVESDGTVLLPGRLFADISRLLGSGQSEIEYLASERKVSVK
                     SGSSSFNLRTLNEKDFPPLPDASGETISLGRESLLETIELVARAASRDDMRPVLTSVQ
                     VLASGNELTMVATDSYRLAVKQTHLAEAVSVELDRNIPANALRELSRLLSASESDSVR
                     LSVTDRLVVFNVDGSILSTNTVDGQFPKYQQLFPETWDHDVRLPRTELLESVRRVSQM
                     AQKNRPLKIALTPGELTISAETPDLGDAEEKMPANFDGEELAIGFNHEFFRDGLEAIP
                     GDEVLLRLISPLRPGLLQPVDSEDFRYLVMPIRLNE"
     gene            complement(1268..2689)
                     /gene="dnaA"
                     /locus_tag="PLE93_03400"
     CDS             complement(1268..2689)
                     /gene="dnaA"
                     /locus_tag="PLE93_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNH86126.1"
                     /translation="MSSERSPASLDQPFSDMRAATSSNRESKAGSRKSLPGSARAVKE
                     WETIQPTLKRRLFDSAWRMWIEPVTPVAVRGTTLYLQAPDPIRTWVERRYIEVITETL
                     VDSSSHLTAVSFENPPEPERVPAPTRPAGPNPTHDFDRFVIGPGNHLAHAAALAVAEA
                     PSESYNPLFLHGPPGLGKTHLLGAIANYLALHVPELNVLYTNAESFTAEFVTALRDQG
                     TDEFKRRYRNLDVLLIDDIQFIAGKPHTEEEFFHTFNALHDAGSQIVLSADRSPGEIS
                     TLADRLSDRFEWGLTVPIEAPNLATRLTVLRHLVTEAGLEVADQDALTLLARRVQSNL
                     RQLRGALTRTIAESSLTSAPITSELITQVFPATESAPAIQITPETIRSTVAAHYRTDA
                     DTLQSRRRDRNTATARHVAIYLTREITSLSLPQIGGLYGDRDHTTVLNSIERVEQSVG
                     QDAEITNAVEILRAQIHSPGENS"
     gene            2859..2993
                     /gene="rpmH"
                     /locus_tag="PLE93_03405"
     CDS             2859..2993
                     /gene="rpmH"
                     /locus_tag="PLE93_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNH86127.1"
                     /translation="MKRTYQPKKRKRAKTHGFRARMSSKGGREVIRRRRRKGRKRLAV
                     "
BASE COUNT          584 a          886 c         1110 g          583 t
ORIGIN      
        1 gatcacgtgc ctactcgttg agacggatcg gcatgaccag gtagcggaag tcttcactgt
       61 cgaccggctg cagcagaccg ggccgcaggg gagaaatcag tcgcagcagg acttcgtcac
      121 cgggaatcgc ctcgagaccg tcgcggaaga actcgtggtt gaaaccgatc gccagttctt
      181 cgccgtcgaa gttcgccggc atcttctcct cggcgtcgcc caggtccgga gtctcggccg
      241 agatcgtcag ctcgccgggg gtgagggcga tcttcaaggg ccggttcttc tgggccatct
      301 ggctgacccg gcgaaccgat tcaaggagtt ccgtccgcgg cagccgtacg tcgtgatccc
      361 aggtttccgg gaagagctgc tggtacttgg ggaactggcc gtcaaccgtg ttggtcgaaa
      421 ggatcgagcc gtcgacgttg aagacgacga gccggtcggt caccgagagg cgaaccgaat
      481 ccgattcgga agccgaaagc aggcgcgaga gttcacgcag cgcgttggcg ggaatgttcc
      541 ggtcaagttc gaccgaaact gcttccgcca ggtgagtctg cttcacggca agccggtagg
      601 agtcggtggc caccatggtc agctcgtttc cggaagcgag cacctggaca ctggtcagca
      661 ccgggcgcat gtcatcgcgg gaggcggcac gggcaaccag ctcgatcgtt tcgagcagtg
      721 actcccggcc gagcgagatc gtttctccgc tggcgtcggg aaggggcggg aaatccttct
      781 cgttgagggt gcgaaggttg aagctcgagg atcccgactt gacgctcacc ttccgctcgg
      841 aagccaggta ctcgatctcg gactgtccgg acccgagcag acgggaaatg tcggcgaaga
      901 gccggccggg aaggaggacg gtgccgtcgg actcaaccga cccctcgaca tggacgggaa
      961 cccgcaggct caggttgctg tcggttgaac tgaaggagag gcgcccgtcg gtcgcctcga
     1021 ccaggattcc gccgagagcc ggattgactc caccggtcgc gagcgtcctg gcgaccaggg
     1081 cgagtttgcc tgaaagctcg ttgttcttga tcgagaactt caaatccgat tacctcgttt
     1141 aagtttggta gttctgtgga gtaaagcgca gaaccggtgg aagtggcttg gggaagccaa
     1201 aatcactgtg gggagaaatg tggaaaaagg gattctttca gcgcgttcgg cggctggaaa
     1261 agacatctca ggagttttcc ccggggctgt ggatttgagc ccggagaatt tcgacggcgt
     1321 tggtgatctc ggcgtcctgg ccaacagact gttcgacccg ctcgatcgag ttgagaacgg
     1381 tggtgtggtc ccggtccccg tagagcccgc cgatctgggg cagggaaagc gaggtgatct
     1441 cccgggtcag gtagatcgcg acatgcctgg ccgtggcggt gttccggtcc cggcggcggg
     1501 actggagggt gtcagcgtca gtcctgtaat gggcggcaac cgtgctgcgg atcgtctccg
     1561 gcgtgatctg gatggccgga gcagattcgg tcgcgggaaa gacctgggtg atcaactcgg
     1621 aggtgatcgg agcggaggtc agcgaagatt cggcaatcgt gcgggtcagt gctccccgga
     1681 gctgacggag gttggactgc acccggcggg ccagcagggt aagggcgtcc tgatcggcta
     1741 cctcgagtcc ggcttcggta accaggtggc gaagaacggt gagccgtgta gccaggttcg
     1801 gcgcttcgat cggcactgtg agcccccatt cgaaacggtc gctgagccgg tcggcgaggg
     1861 tcgagatctc gcctggcgag cggtcagcgg aaagaacgat ctggctgcct gcgtcatgca
     1921 gggcattgaa ggtgtggaag aactcttcct cggtgtgggg cttgccggcg atgaactgga
     1981 tgtcatcgat cagcagtacg tcgagattgc ggtagcgccg tttgaactcg tcggttccct
     2041 ggtcgcggag ggcggtgacg aattcggcgg tgaaggactc ggcattcgta tagaggacgt
     2101 tcagttcggg aacgtgaaga gccaagtagt tggcgatcgc accgagcagg tgggtcttgc
     2161 cgaggccggg gggaccgtgc aggaagagcg ggttgtatga ctcggaaggg gcttcggcga
     2221 ccgccagggc agcggcgtgg gcaagatgat tgccggggcc gatcacgaag cgatcgaagt
     2281 catgggtcgg gttgggaccg gccggccggg ttggcgccgg gacccgttcg ggttcgggtg
     2341 gattctcgaa gctgaccgcg gtgaggtgcg aactcgagtc gacgagcgtc tcggttatta
     2401 cctcgatgta tcgccgctcc acccaggtac gaatcggatc cggagcctga agatagagcg
     2461 tggttccgcg gaccgcgacg ggagtaaccg gttcgatcca catccgccag gccgagtcaa
     2521 agaggcgccg cttgagggtc ggctggatgg tctcccattc cttcacggca cgagccgagc
     2581 cgggaagcga ctttcggctt cctgccttgc tttcacggtt tgaactggtg gcggctctca
     2641 tgtcggaaaa gggctgatcg aggctggccg gggaacgctc gctgctcaat ctccaccctg
     2701 cctgccgacc atagcgccgg tccatgggag gccgcgttcc ggacccgcaa atcgcgcaga
     2761 aaatttcttg gagccgggtt tccgcccggc aggaggctgg cagcgggcgc agggcgggaa
     2821 ccatgaggcg cctgatcccc ttatactgcc cggctccaat gaagcgtacg taccagccaa
     2881 agaagcgcaa gcgcgcaaag acccacggct tccgggcccg catgagctcc aagggcgggc
     2941 gcgaggttat tcgccgccgc cgccgcaagg gccgcaagcg gctcgccgtc tgaccatgtc
     3001 agcaaacggc gctggttctc ccaggcgccg ccggctctcc cgcagcggag acttcaaacg
     3061 ggcttaccgc gaagggtcgt caaaggcgac ccgttacctc gttctctacc ggttcgatcg
     3121 ctcggccgat gatgaatccg agattcgcct cggggtcagc gtt
//
LOCUS       DAOQFI010000139         3162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15040_length_3162_cov_3.607982, whole genome shotgun sequence.
ACCESSION   DAOQFI010000139 DAOQFI010000000
VERSION     DAOQFI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15040_length_3162_cov_3.607982"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..598)
                     /gene="rplF"
                     /locus_tag="PLE93_03410"
     CDS             complement(65..598)
                     /gene="rplF"
                     /locus_tag="PLE93_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNH86128.1"
                     /translation="MSRIGKKPIDLPDGVEIDIKPGLITVKGPKGELTQEVSTDMTVK
                     TEEGVISVERPTNRPNHRALHGLTRSLIANMIVGVTDGYTKVLEIQGVGYRAAAKGKN
                     LELALGYSHPVSIEAPEGIEFELPQPTEIIVKGIDKQKVGQVAANIRESRPPEPYKGK
                     GIRYRGEHVARKVGKRV"
     gene            complement(609..1004)
                     /gene="rpsH"
                     /locus_tag="PLE93_03415"
     CDS             complement(609..1004)
                     /gene="rpsH"
                     /locus_tag="PLE93_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNH86129.1"
                     /translation="MLTDPISDYLTRIRNGLSSGLATVEVPSSKLKLEVSRILTEQGY
                     ISGYEKSAADVGEKITVELKYTKDHQPVITGLERVSRPGRRRYVGHGEVPRVHGGTGT
                     AIITTSHGVMTGHEAKKEGVGGEVLAYVW"
     gene            complement(1009..1194)
                     /locus_tag="PLE93_03420"
     CDS             complement(1009..1194)
                     /locus_tag="PLE93_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNH86130.1"
                     /translation="MAKTSQKVRQQRKAKFSTREYNRCRRCGRPRAYYRKFGICRICL
                     REAAHEGYIPGMTKSSW"
     gene            complement(1404..1973)
                     /gene="rplE"
                     /locus_tag="PLE93_03425"
                     /pseudo
     CDS             complement(1404..1973)
                     /gene="rplE"
                     /locus_tag="PLE93_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932832.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            complement(1982..2308)
                     /gene="rplX"
                     /locus_tag="PLE93_03430"
     CDS             complement(1982..2308)
                     /gene="rplX"
                     /locus_tag="PLE93_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNH86131.1"
                     /translation="MRIRTDDTVQVISGKEKGKQGKVLRTDPKAGTVYVEDLNMIKRH
                     QRAQPSPDGRGQGKEGGIIEMEGPIHVSNVALVDPTDNKPTRIGVRITDDGVRQRYSK
                     RTGEAI"
     gene            complement(2308..2676)
                     /gene="rplN"
                     /locus_tag="PLE93_03435"
     CDS             complement(2308..2676)
                     /gene="rplN"
                     /locus_tag="PLE93_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNH86132.1"
                     /translation="MIQQESRLRVADNSGARELLCIRVKGGSHRKYGSIGDVITATVK
                     QASPQGNVKKGEVVQAVIVRTKKEIGRQDGTYIAFDENAAVLIDAQNNPRGTRIFGPV
                     ARELRDRDFMKIISLAPEVL"
     gene            complement(2680..2985)
                     /gene="rpsQ"
                     /locus_tag="PLE93_03440"
     CDS             complement(2680..2985)
                     /gene="rpsQ"
                     /locus_tag="PLE93_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNH86133.1"
                     /translation="MKKERTGDGVGTPRAEKTRTGKPKERQGKVVSDKTDKTITVQID
                     TARRHPVYEKIVRQSHKIHAHDERNEAHEGDVVRVIETRKLSRTKHWKLVEVVEKAR"
BASE COUNT          434 a          999 c         1033 g          696 t
ORIGIN      
        1 ccggacacct tggcccggac ccggtagcgg cggcgctgcc ttctctggcg gctggttgtg
       61 atgctcatac tcgcttgccg accttcctgg cgacgtgttc gccgcggtaa cggatgccct
      121 tgcccttgta aggctccggc ggacggctct cgcggatgtt tgccgcgacc tggccgacct
      181 tctgcttgtc aatccccttg acgatgatct ccgtcggctg gggaagttca aattcgatgc
      241 cctcgggagc ctcgatcgag accggatgcg aatatccgag cgccagttcg aggttcttgc
      301 ccttggccgc ggcacggtaa ccgacgccct ggatctccag gaccttggtg tagccgtcgg
      361 tgacgccgac gatcatgttg gcgatcaggc tgcgggtcag accgtggagc gcacgatggt
      421 tcgggcggtt cgtcggtcgc tcgaccgaga tcacgccttc ctccgtcttc acggtcatgt
      481 cggtcgagac ttcctgggtg agctcgccct tcgggccctt gaccgtgatc agtccgggct
      541 tgatgtcgat ctcgacccca tcggggagat cgatcggctt ttttccaatt cgactcatga
      601 attcctcgtt accagacgta ggcgagaacc tcgccgccga cgccttcctt cttggcttcg
      661 tgaccggtca tcacaccgtg agaggtggtg atgatcgcgg tgccggtgcc gccgtgaacc
      721 cgggggacct cgccgtggcc gacgtaacga cggcggccgg ggcgggaaac ccgctcgagt
      781 ccggtgatca ccggctgatg gtccttcgtg tacttgagct cgaccgtgat cttctcgccc
      841 acgtcggcag cgctcttctc gtagcccgag atgtagccct gctcggtgag gatgcgggag
      901 acctccagct tcagcttcga gctgggcacc tcgacggtgg ccaggccgga gctgaggccg
      961 ttgcgaatgc gggtcaggta gtcggaaatc ggatcggtaa gcatttgact accagctcga
     1021 cttcgtcatg ccggggatgt agccctcatg ggcggcctcg cgcaggcaga tccggcaaat
     1081 gccgaacttg cggtagtacg cacgggggcg gccgcaccgc cggcaccggt tgtactcgcg
     1141 ggttgaaaac ttcgccttgc gctgctggcg caccttctgt gacgtcttgg ccattaggcc
     1201 tggtcctcct gctcttcggc ggctgcgtct tcggcggcat cgtccgatgc ctcctcggcc
     1261 gcgtcatttt cagtgtctgc tgcttctgcc ggggcctctt cttcggccgg cgcttcggtg
     1321 gagccggctc catcagttgc ctccgcagcc ggctcatccg gggcggtgct ttcttcggca
     1381 ccctcgggga ccggacggcc ttccttggcg aaaggcatgc ccaggccgag cagcagctcg
     1441 aaagcctcgt agtccgtcgg tgcggacgtg gtaatcgtga tgtcgagacc gcgggtctcg
     1501 tcaaccgagt cgtaatcgat ctcggggaag atcagctgtt ccttgacacc catggagtag
     1561 ttgccgcgac cgtcgaatga ggtcgccttg agtccgcgga agtcacggat gcgcgggatc
     1621 gcgatcgagt tcagtcgatc gaggaactcc cacatccggg cgccccggag ggtcaccgag
     1681 gcgccgaccg gcattccctc gcgaagcttg aaggacgcga tcgacttctt ggcgatccgg
     1741 gtgttcggca tctgaccggc gatcgtggcg agctcttcga tcgccttttc gcgggacttg
     1801 gagtcaaccg aggagccgag gcccatgttc agggtgatct tctgaaggcg cggggcccgc
     1861 atcggagtcg agtagccgaa ggtctccatc agcttcggga gaacctcgtt gatgtaggtg
     1921 tcccggagcc gcggcggcgg cactgcctgt tgtgttgtcg tagctgcttc catgatcttc
     1981 tctaaatggc ctcgccggtg cgcttcgagt agcgctggcg aacgccgtcg tcggtgatgc
     2041 ggacgccgat tcgggtcggc ttgttgtcgg tcgggtcgac cagggccacg ttcgacacgt
     2101 ggatcggacc ctccatctcg atgatcccgc cttccttgcc ctggccccgg ccgtccggcg
     2161 agggctgggc gcgctggtgg cgcttgatca tgttgaggtc ttcaacgtac acggttccgg
     2221 ccttggggtc ggtcctcaga accttgccct gcttgccctt ctccttgccg ctgattacct
     2281 gcacggtgtc gtcggtgcgg atacgcatca cagcacctcc ggggcgagcg agatgatctt
     2341 catgaagtcg cggtcacgca gttcgcgggc gaccggtccg aagatgcggg taccgcgcgg
     2401 gttgttctgc gcgtcgatca gcacggcagc gttctcgtcg aaggcgatgt aggtgccgtc
     2461 ctggcggccg atctccttct tggtccggac gattacggcc tggacgacct cgcccttctt
     2521 gacgttgccc tgcgggctgg cctgcttgac ggtcgcggtg atcacgtcac cgatcgatcc
     2581 gtacttgcgg tgcgagccac ccttgacccg gatgcagagg agttcacggg cgccggagtt
     2641 gtcggcgacg cggagtctgg attcctgctg gatcaccgtc tacctcgcct tctccacgac
     2701 ctcgaccagc ttccagtgct tggtcctgga aagcttgcgg gtctcgatca cgcggaccac
     2761 gtcaccctca tgggcttcgt tccgttcgtc atgcgcgtgg atcttgtgcg actggcggac
     2821 gatcttttcg tagaccgggt gacgccgggc ggtgtcgatc tggaccgtga tggtcttgtc
     2881 ggttttgtcg gaaacgacct tgccctggcg ctccttcggc ttgccggtac gggtcttctc
     2941 ggcccgcggg gtaccgacac cgtcaccggt gcgttccttc ttgatctcct cgcggcgatt
     3001 gcgacggatc tgcgccttct tcttccgggc agcgacgcga tcggactggc gctgctcgcc
     3061 cgaacgttcg gggccggcct cgccggattc acgcgacttg agcacgcggc ggcgttcctt
     3121 ccaggggagc tcgtccagcg gatcgggctc agcctcggcg gc
//
LOCUS       DAOQFI010000140         3155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15094_length_3155_cov_3.991613, whole genome shotgun sequence.
ACCESSION   DAOQFI010000140 DAOQFI010000000
VERSION     DAOQFI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15094_length_3155_cov_3.991613"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLE93_03445"
     CDS             complement(<1..315)
                     /locus_tag="PLE93_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86134.1"
                     /translation="MKRNYFLKTIGASDHLLSDDWTQTDPHLLSSVRFPKRPSSIKRG
                     DFLVYYATGEQRIFAIGRAVGDGADAAHEIAPGEARWPYLLKIQVFLLIPKLALAPKW
                     DSM"
     gene            complement(407..964)
                     /locus_tag="PLE93_03450"
     CDS             complement(407..964)
                     /locus_tag="PLE93_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH86135.1"
                     /translation="MTIPAQEWTELSQQEKRARALDAADRLFAREGLDAPMPALAEAL
                     GIGVGSIYRQVGTKNELIGALVIERSKTLCRRFDATLAEPDPWQAMTAAVHETVDDCL
                     NDALTQSAWDQAAIVSDEVRAARQQATDSLAAMFERARRSGAITAEASPEDLRLLFFA
                     LREFSSVGSAQAHRFTDLVLRGIKS"
     gene            982..2871
                     /gene="ilvD"
                     /locus_tag="PLE93_03455"
     CDS             982..2871
                     /gene="ilvD"
                     /locus_tag="PLE93_03455"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNH86136.1"
                     /translation="MPDLRSKTVTHGRNMAGARALLRASGVAREDFGKPIVAVANSYT
                     QFVPGHTHLKPVGEVVSAAIKEAGGVPLEFNSIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLNAALRLNIPTVFVSGGPMEGGEATLVDGTV
                     RKRVNLISAIADAVADEVSDEDIAIIEENACPTCGSCSGMFTANSMNCLTEALGLSLP
                     GNGSTLATHTARKALYESAGRTAVEIAGKYYGEDDESVLPRSVATRDAFENAMTLDIA
                     MGGSTNTVLHLLAAAREAGVDFEMSDIDELSKRVPCICKVAPNGHFLMEDVHRAGGIP
                     AILGELRRGGLLNEEVHTVHSPDIDTWLGSWDIRGPKPSEIALDLFHAAPGCKRSSSA
                     FSQSERWESLDTDAAEGCIHDVEHAYSKDGGLAILYGNIAERGCVVKTAGVDESILKF
                     NGPAFVVESQDDAVDAILSGRVKSGDVVVIRYEGPRGGPGMQEMLYPTSYLKGLGLGK
                     ECALLTDGRFSGGTSGLSIGHVSPEAAAGGAIALVETGDEIAIDIPNRTISLEVEEET
                     LAARREAIESSGGYQPVDRDREVSPALRAYAMMATSADTGAVRDVDGVERLVNAARLL
                     DSEQV"
     gene            2877..>3155
                     /locus_tag="PLE93_03460"
     CDS             2877..>3155
                     /locus_tag="PLE93_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNH86137.1"
                     /translation="MLRIGHKGADALVPGNTVASFEKAVEVGVDLIEFDVLWTEDGHP
                     SIPAGKRSELVVAHDWHAAAAAGERGERLTLEAALEAFARPPLDRVRIN"
BASE COUNT          524 a         1024 c         1052 g          555 t
ORIGIN      
        1 cattgagtcc catttgggtg cgagcgccag cttcggaatc aacagaaaga cttgaatctt
       61 cagcagatat ggccatctgg cttcgcccgg tgcgatctcg tgagcggcat ccgcaccatc
      121 gccgaccgcc cttccaattg caaagatgcg ttgttcgcca gttgcgtagt agacgagaaa
      181 atcacccctc ttgatgctgg acgggcgttt cgggaagcgg accgaggaga gcaggtgagg
      241 gtcggtctgg gtccagtcat cactgagcaa gtggtcgctc gctccgatgg tcttgaggaa
      301 gtagtttcgc ttcatgagga ttctctcttg gtagctggac tcatttagtc gttttcactt
      361 gccctttctg acaggtcatc ggccgttgtt catgagaagg ctggaactag ctcttgattc
      421 cgcgcaggac gaggtcggtg aagcggtggg cttgggctga tccgaccgag ctgaattctc
      481 ggagggcgaa gaagaggagg cggaggtctt cgggtgaggc ttccgcggtg atcgctccgg
      541 atcgacgggc cctttcgaac atcgcggcca gggagtcggt tgcctgctgg cgggcggcgc
      601 ggacttcgtc gctgacgatt gcggcctggt cccaggctga ctgggtgagc gcgtcgttca
      661 ggcagtcgtc gaccgtttcg tgaaccgctg ccgtcatcgc ctgccaggga tcgggttcgg
      721 cgagagtggc atcgaatctc cggcagaggg tctttgaccg ctcgatgacc agggcgccga
      781 tcagctcgtt tttggtgccg acctgccggt agatgctgcc gaccccgatc ccgagtgcct
      841 cggcaagggc gggcatcggg gcgtcgagac cctcccgggc aaagaggcgg tcggccgcat
      901 ccagcgcccg ggcccgcttc tcctgctggg aaagttccgt ccactcttgt gccggaatgg
      961 tcattccgct atcttagcca catgccagat ctgagatcga agaccgttac tcatggacgc
     1021 aacatggccg gggcccgggc gcttctgcgc gcttccggag tggcccgtga ggacttcggc
     1081 aagccgatcg tggcggtcgc caactcgtac acccagttcg ttcccggtca cacccacctg
     1141 aagccggtcg gcgaggtcgt ctccgcggcg atcaaggagg ctggcggtgt gccgctcgag
     1201 ttcaactcga ttgcggtcga tgacggaatc gcgatgggcc acggcggcat gctctactcg
     1261 ctgccatccc gcgacctgat cgccgacagc gtcgagtaca tggtcaacgc ccactgtgcc
     1321 gatgcgatgg tctgcatctc gaactgcgac aagatcacgc cgggcatgct gaacgcggcc
     1381 ttgcggctca acatcccgac cgtcttcgtc tccggcggcc cgatggaagg cggcgaggcg
     1441 accctggtcg acggcaccgt ccgcaagcgg gtcaacctga tctcggcgat cgccgacgcg
     1501 gtcgccgacg aggtctccga cgaggacatc gcgatcatcg aggagaacgc ctgcccgacc
     1561 tgcggttcct gctccggcat gttcaccgcc aactcgatga actgcctgac cgaggcgctc
     1621 ggtctctcgc tgcccggcaa cggttcgacc ctggcgaccc acaccgcccg caaggcgctc
     1681 tacgaatcgg ccggccgcac cgcggtcgag atcgccggca agtactacgg cgaagacgac
     1741 gagtcggtgc tgccccgatc ggtcgcgacc cgcgatgcat tcgagaacgc gatgaccctg
     1801 gacatcgcca tgggcggctc gaccaacacc gtcctgcacc tgctggcggc ggcccgcgag
     1861 gcgggggtgg acttcgagat gtccgacatc gacgagcttt cgaagcgggt gccctgcatc
     1921 tgcaaggtcg cgccgaacgg gcacttcctg atggaggacg tccaccgggc cggcggcatt
     1981 cccgccatcc tcggcgaact gcgccgcggc ggactgctga acgaggaagt ccacaccgtc
     2041 cattcgccgg acatcgacac ctggctcggc agctgggaca tccgcgggcc gaagccctcc
     2101 gagatcgcgc tcgacctctt ccacgcggcg cccggctgca agcgctcttc gtcggccttc
     2161 tcgcaatcgg agcgctggga gtcccttgac accgacgcgg ccgagggctg catccatgac
     2221 gtcgagcacg cctactcgaa ggacggcggc ctggcgatcc tttacggcaa catcgccgag
     2281 cgcggctgcg tggtcaagac ggccggggtc gacgaatcga tcctcaagtt caacgggccg
     2341 gccttcgtgg tcgaatccca ggacgacgcg gtcgacgcga tcctcagcgg ccgggtcaag
     2401 tccggcgacg tggtcgtgat ccgttacgaa ggcccgcgtg gcggtcccgg catgcaggag
     2461 atgctctacc cgacctccta cctgaagggt ctggggctcg gaaaggaatg tgccctgctc
     2521 accgacgggc gtttctccgg tggcacctcc ggcctttcga tcggccacgt ctcccccgag
     2581 gcggcggccg gcggcgcgat cgccctggtc gagaccggtg acgagatcgc gatcgacatc
     2641 cccaaccgga ccatctcgct cgaggtcgag gaggaaaccc tggccgcccg acgcgaagcg
     2701 atcgagagct ccggcggcta ccagccggtc gaccgtgatc gcgaagtttc accggccctg
     2761 cgggcctacg cgatgatggc gacctcggcc gacaccggcg cggtccgcga cgtcgacggg
     2821 gtggagcgac tggtcaacgc cgcccgcctg ctcgactccg agcaggtgta gctgcggtgc
     2881 ttcgtatcgg tcacaagggg gccgatgcgc tggtgccagg gaacacggtc gcttccttcg
     2941 agaaggcggt cgaggtcggg gtcgacctga tcgagtttga cgtgctctgg accgaggacg
     3001 gccacccttc gatccccgcg ggcaagcgaa gcgagctggt cgtcgcccat gactggcacg
     3061 ccgccgcggc cgccggggaa aggggggaga ggctgaccct ggaagcagct ctggaggcct
     3121 tcgcccggcc gcctctggac cgggtccgga tcaac
//
LOCUS       DAOQFI010000141         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15127_length_3150_cov_3.434895, whole genome shotgun sequence.
ACCESSION   DAOQFI010000141 DAOQFI010000000
VERSION     DAOQFI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15127_length_3150_cov_3.434895"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1345)
                     /locus_tag="PLE93_03465"
     CDS             complement(125..1345)
                     /locus_tag="PLE93_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNH86138.1"
                     /translation="MAPLPSKLTSPPELWRRRIPLLAASACLLLVVLMSGSSSAADTQ
                     SKLDKAEKKIEEGQQREGVLTEKLDQLGERVDSMQAAVDALREAEQEAAAKLQQAEAD
                     LKLAIERLQTSLEELRETRRRLKVSLQVLRQRLVDIYVSGTPSIASMVLAANEYSDLV
                     EMGPYLEAIQSRDESLVQRVRELKNEARKIVEARRDSKETIQTSRDSIAAETARLASA
                     RSETESQVASLASAKAASEEALNEVQDGIAQEEEVAADLREKLTKELGTATSVPFSVG
                     PASTPSSNSMIWPIDGTLTSPFGPRWGRTHEGLDIAAPAGTPIAAAASGTVVLMQSEA
                     ESGGYGLFTCVDHGGGLSTCYAHQSEFGTSVGASVKQGEVIGYVGNTGNSFGDHVHFE
                     VRINGAATDPMAYL"
     gene            complement(1418..2626)
                     /locus_tag="PLE93_03470"
     CDS             complement(1418..2626)
                     /locus_tag="PLE93_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fenitrothion hydrolase"
                     /protein_id="HNH86139.1"
                     /translation="MPANSSAVQFLSGLIGVLLLAAVLYAGIEGTEAADRNFSLTFVF
                     VTVWLGFPVLSVVFGNVFAAFSPWRAIGRAYGWLAQRLAPGAVRHVEYPEKVGRWPAA
                     VGLVAFVWLEIIYGATGAAVVLSPEIVAKATVIYSVYTLVMMGVFGVEKWSSRGEFFS
                     VYFGMFASMSSFRKREGKLERRRWLSGTTDWANVPGSLALVVSAIGVTTFDGAQEGAL
                     KTPIEWTQERIGDLGFAATVSVRAGDTIFLLITIGIVALIYLLGVKGMTRVDAAPPFK
                     VLRSRFAHTLIPIALAYLVAHYFSLFVFQEQAQFTFLLSDPLGDGSNLFGTATSGIDY
                     TLIGPELVWYVQVGALIAGHVTALVLAHDRALLIWPDYRLATRSQYWMLAVMLCFTSL
                     GLFLLSFANQ"
     gene            complement(2803..>3150)
                     /locus_tag="PLE93_03475"
     CDS             complement(2803..>3150)
                     /locus_tag="PLE93_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86140.1"
                     /translation="DQGQATSKTTGNTGPTSAPQSKPPQPEKPVVPVYRVVDGQPAGG
                     VQKLEANEGDNIRFVVKSDVDDEVHVHGFDVSEPVGPGKPARFDFKADFTGVFEIELE
                     NSAVPIIELQVNP"
BASE COUNT          602 a          947 c          982 g          619 t
ORIGIN      
        1 ggctcgagct ggactcgttg ctcaaagagc agttcgagat cgagcactca acgcttcaac
       61 ttgagcacgc gggcgatggg aagcctctgg agatcacccg gccggatggt ttgacggccc
      121 cgggttagag gtaggccatc gggtcggttg ctgccccgtt gatccgtact tcgaagtgaa
      181 cgtggtcacc aaagctgttg cccgtgtttc cgacgtaccc gatcacttct ccctgcttga
      241 cagatgcccc gaccgatgtt ccgaattcgg actgatgggc gtagcaggtg gacaacccac
      301 cgccatgatc cacgcaggtg aagagcccgt agccgccgct ctctgcttct gactgcatga
      361 gcactaccgt ccccgatgcc gcggcggcga ttggtgtgcc ggcgggggcc gcgatgtcca
      421 ggccttcatg cgttctgccc catcttgggc cgaacggcga agtaagagtt ccgtcgatcg
      481 gccagatcat gctgttggaa ctgggtgttg acgccggtcc cacggagaac gggaccgacg
      541 tggctgtacc cagctccttg gtgagcttct cgcgcaggtc cgccgcgact tcctcttcct
      601 gagcgattcc gtcctgaacc tcgttcagtg cctcttcgct cgcggccttc gccgacgcaa
      661 gagatgcgac ctgggattcg gtttcacttc gagcactcgc caatcgcgcg gtctcggcgg
      721 cgatcgagtc gcgagaagtt tggatggttt cctttgagtc gcgtcgggcc tccacgatct
      781 tgcgcgcctc gttcttcagt tccctgactc tctgaacgag agactcgtct cgcgactgga
      841 tcgcttcgag gtacggtccc atctcgacca gatcgctgta ctcgttggcg gccagcacca
      901 tcgaggcaat gcttggtgta cctgagacgt agatgtccac caacctctgt ctgaggacct
      961 gaagggacac cttcagacgt cttcgagttt cgcggagctc ttcgagggaa gtctggagtc
     1021 gttcgatcgc gagtttcaag tcagcttctg cctgctggag tttcgccgcc gcttcttgtt
     1081 cggcctctcg cagtgcgtcg accgcggcct gcattgagtc gacccgctca ccgagctgat
     1141 caagtttctc ggtcaggact ccctcccgct gttggccttc ttcgatcttc ttctctgcct
     1201 tgtccagctt cgattgcgtg tcggctgccg atgaactgcc cgacatgagc accacgagga
     1261 gcaggcaagc ggaagcggcg agcaggggaa tgcgtcgtct ccacagttcg ggtggagagg
     1321 tgagtttcga ggggagggga gccaagctcc tacggactgt agtagttaga tcttgggttc
     1381 agcctgcgag gtgcggccct gcaatgaagc gcccgcttta ttggttcgca aatgagagca
     1441 ggaagaggcc cagactcgtg aagcagagca tcaccgcgag catccagtac tgagatctgg
     1501 tggccagtcg gtagtcgggc cagatcagaa gtgcgcggtc atgggcgagg acgagcgcag
     1561 tcacgtggcc ggcgatgagt gccccgacct ggacgtacca gacaagttcg ggcccgatca
     1621 gggtgtagtc gatccctgat gtcgctgtcc cgaacagatt cgatccgtcg cccagcggat
     1681 ctgagagcag aaaggtgaac tgggcttgct cctggaacac gaagagactg aagtagtggg
     1741 ccacgaggta tgccagcgcg atcggaatga gtgtgtgagc aaagcggcta cgaagcacct
     1801 tgaacggtgg cgccgcatcc acccgggtca ttcccttgac tccgaggagg tagatgagtg
     1861 ccacgattcc gatcgtgatc agcaggaaga tggtgtcgcc agcccggacc gagactgttg
     1921 ccgcgaaccc caggtcaccg attcgttcct gcgtccactc gatcggggtt ttcagcgcac
     1981 cttcctgcgc accgtcgaag gtggtcacac cgatggcaga aacgacgagc gccagcgatc
     2041 ccggcacgtt tgcccaatcg gtggttcccg agagccaacg tcgacgctcg agctttcctt
     2101 cccgctttcg aaacgacgac atcgaggcga acatgccgaa gtagaccgag aagaactctc
     2161 cacgtgagct ccatttctca acgccgaaca cgcccatcat caccagggtg tagaccgagt
     2221 agatgaccgt cgccttggcc acgatttcgg gggagagaac cacggccgcg cccgtcgctc
     2281 cgtagatgat ctcgagccag acgaaggcga cgagtccgac cgccgccggc cacctgccga
     2341 ccttctccgg gtactcgacg tgccttacgg ctcccggcgc cagtcgctgc gcaagccatc
     2401 cataagccct gccgattgcc ctccacggag agaacgcggc gaagacgttc ccgaagacca
     2461 cactcaggac ggggaagccg agccagacgg ttacgaagac gaaggtgagg gagaagtttc
     2521 ggtcggcagc ttcggttcct tcgatccccg cgtagaggac agccgcgagt agaaggacgc
     2581 cgatcaggcc tgagaggaac tgaactgccg aggagtttgc cggcactgag agccaggtcg
     2641 ctgcgggtcg acccggagca tcttcgaacc tgggtttgcg ccaggccgag gccagcgcga
     2701 aaaatgagac gacaaggact acgccggcgc cccacgcgaa aagccaaaca ggaatcggca
     2761 ggtcaccccg cgagaccagg gcatgcgcga ggggcagact gatcaagggt tgacctggag
     2821 ctcgatgatc gggactgctg agttctcgag ttcgatttcg aagactccgg tgaaatcggc
     2881 tttgaagtcg aaccgggccg gcttgccggg acctacgggt tcgctgacat cgaatccgtg
     2941 gacgtggacc tcgtcatcaa catcggactt gaccacgaat cgaatgttgt cgccctcatt
     3001 ggcctcgagc ttctgaactc cgcccgcggg ctgtccgtcg acaacccggt aaacgggaac
     3061 gaccggcttc tcaggctggg gcggcttgct ttgcggggcc gaagtgggcc cggtgttccc
     3121 ggtggtcttg gatgtcgcct gtccctggtc
//
LOCUS       DAOQFI010000142         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15174_length_3144_cov_4.303334, whole genome shotgun sequence.
ACCESSION   DAOQFI010000142 DAOQFI010000000
VERSION     DAOQFI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15174_length_3144_cov_4.303334"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            484..1278
                     /locus_tag="PLE93_03480"
     CDS             484..1278
                     /locus_tag="PLE93_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH86141.1"
                     /translation="MNAGRAPMRVAVIDTDSGFIRVLSNRMDAAGWQYRVLASGVPPE
                     ELVAMKINAVVFDPDVIGEGGWEYLERICGMLPDLGVIVCANGGTVSQRVRGLRIGAD
                     DWVNKPCHPEEVIARIEAVVRRRRRHSNEQDAGPLVVGEIEIRPDQFQAFIAGQSLDL
                     TRREFELIQLLAHNEGKVLDRESIYQRVWGYAMAHGDRSVDVFIRKLRTKLERRSPAY
                     TYIHTHFGVGYRFAAESSISAAEATAKVSSDRATDDAGAAAPGVPV"
     gene            1461..2144
                     /gene="phoU"
                     /locus_tag="PLE93_03485"
     CDS             1461..2144
                     /gene="phoU"
                     /locus_tag="PLE93_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNH86142.1"
                     /translation="MTEKIRIQYQEELDHLEQQALGAFDLVDRALGRTLEAIENQDVE
                     LAELVAADDDRIDGRYLEIHQGLITLLATQSPVATDLRLISALLHVIKNAERMGDQCV
                     NICKLIPIVGHEPPSDESMIRLILEMGVQASSQIIQARQAFADRNETAAQDLVRQDDL
                     IDRLNRDCFTLAIEIGDEPDRREWAMTMLLAARAIERIGDNAVDVGEQVAFVVTGLFR
                     EFEDASHPI"
     gene            2157..2510
                     /locus_tag="PLE93_03490"
     CDS             2157..2510
                     /locus_tag="PLE93_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNH86143.1"
                     /translation="MEAAKSQTGGSAGHALRAGPLEVRLDQRTVLADGRGIILTVREF
                     HLLAVLASRPEQVVSREEVYDLVWGGAMPENDRSVDVYVSKLRHKLETALPHWSFIQT
                     HIGFGYCFSPRPLNP"
     gene            2651..>3144
                     /locus_tag="PLE93_03495"
     CDS             2651..>3144
                     /locus_tag="PLE93_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNH86144.1"
                     /translation="MKKWLIAIPLTLALALGVAACGSSDSDSGSSGSSGDAVSGEIAG
                     AGASSQEAAMAAWVAGFQENNPDANIAYDPVGSGGGREQFIAGGTDFGGTDTPMDETE
                     LADVAKRCKPGEYIEIPVYISPIAVPYNVEGVDDLNLSASTLARIFAGDITKWNDQAI
                     AAENP"
BASE COUNT          597 a         1016 c         1006 g          525 t
ORIGIN      
        1 gccgtcggcg gctgccgcgt aggcgggcag ggccagaacg aaaagcgcgg tcagaagggc
       61 aacgagactg ttacggacac gattcacgcc ccgaaaccta ccgcaaaccg caggacgcct
      121 gtggctaccc gactgctccg gcgcgggtgt gcctgttgct cctggcccgg gagttgtgcc
      181 tttgcggcac ctcgggatcc gagggcaggt gagaatgggg cccggcctgg aagatcgagg
      241 agggagttct cggcatatgg cgcacccaac tccgacttcg atccggacca ccgggccgag
      301 aacggagttg ggtgcgctat gtggcgcaaa ttggtacctc gctgttttcg ggactcccgg
      361 ctcgcttcag gtccagagcc gaatcttttc ggggttgtga atgagttgtg aaaatcggct
      421 tgatttcgcg gctttgcgtc cggctacgcc ctacactttt cggtagatga tcggaagctc
      481 aaaatgaacg ccggccgggc tccaatgcgt gttgcggtga tcgacaccga ttccggcttc
      541 atccgggtcc tttcgaaccg gatggacgcg gccggctggc agtaccgggt cctcgcctcg
      601 ggcgtgccgc cggaggaact ggtcgcgatg aagatcaacg cggtcgtgtt cgatccggac
      661 gtgatcggcg aaggcggctg ggagtacctc gagcggatct gcggcatgct gcccgacctc
      721 ggcgtgatcg tctgcgccaa cgggggcacc gtttcacagc gggtccgcgg gctcaggatc
      781 ggggccgacg actgggtcaa caagccctgc cacccggaag aggtgatcgc caggatcgaa
      841 gcggtggtgc gccgtcgccg tcgtcattcc aacgaacagg acgccggtcc gctggtcgtc
      901 ggggagatcg agatccgccc cgaccagttc caggccttca tcgccggcca gagcctcgac
      961 ctgacccgcc gcgagttcga gctgattcaa ctcctcgcac acaatgaggg caaggtcctc
     1021 gaccgcgaat cgatctacca gcgtgtctgg ggctacgcga tggcccacgg cgaccgctcg
     1081 gttgacgtct tcatccgcaa gctccgcacc aagctggaga ggcggtcccc ggcctacacc
     1141 tacatccaca cccacttcgg cgtcggctac agatttgcgg ccgaatcgtc gatttcggcc
     1201 gccgaggcga ccgccaaggt cagctccgac cgggccaccg atgatgcggg cgctgcggcg
     1261 cccggcgtgc cggtctagcg ctgcaccttc tctcactcac gaaaacgggc acccgatcaa
     1321 gggtgaaccc gcttcacagg agttcacaac tcttttacag caggtctacc cgcaggtaac
     1381 aaactggggc ggaagcggtg gaacgcttcg aaccaccagg ccgcccccga aaggcgggaa
     1441 ccaagtcccg gagggtgtga gtgaccgaga agatccgaat tcagtatcag gaagaacttg
     1501 accacctcga gcagcaggct ctcggggcct tcgatctcgt ggaccgggcg ctaggccgca
     1561 ccctcgaagc gatcgaaaac caggacgtcg agctggccga actggttgct gccgacgacg
     1621 accggatcga cgggcgctac ctcgagattc accagggtct gatcaccctg ctggccaccc
     1681 agtccccggt ggccaccgac cttcggctga tttccgctct gctccacgtg atcaagaacg
     1741 cggagcggat gggggatcag tgcgtgaaca tctgcaagct gatcccgatc gtcggccacg
     1801 agccgccttc ggacgagagc atgatccggt tgatcctcga gatgggggtc caggccagca
     1861 gccagatcat tcaggccagg caggccttcg ccgatcgcaa cgagacggcc gcccaggacc
     1921 tggtccggca ggacgacctg atcgaccggc tgaaccgcga ctgcttcacc ctggcgatcg
     1981 aaatcggcga cgagcccgac cgccgtgaat gggcgatgac gatgttgctg gccgcgcgtg
     2041 cgatcgagcg gatcggcgac aacgcggttg atgtcgggga acaggttgcc tttgtggtga
     2101 ccggcctgtt ccgggaattc gaggacgcgt cccacccgat ctgagccggg ccgagcgtgg
     2161 aagcagcaaa gtcccagacg ggcggttccg ccggccacgc gctgagggcc gggcccctgg
     2221 aagtccggct cgatcagcgg accgtcctcg ccgacggcag gggaatcatc ctcacggtcc
     2281 gggagttcca cctgctcgcg gtcctggcca gccggccgga acaggtcgtg agcagggaag
     2341 aggtctacga cctggtctgg ggcggcgcga tgcccgaaaa cgaccggtcg gttgacgtct
     2401 acgtctcgaa actgcgccac aagcttgaaa ccgccttgcc tcactggagc ttcatccaga
     2461 cccacatcgg ctttggctac tgcttttcgc cgcgaccgct gaacccctga ctcgcggcaa
     2521 gtgacgggct tttcacctct tttcacaact ttcacaagga agtaacaccg cgcgaaatct
     2581 ccatctattg actgaagttc attactgcgc aacccctcga aagaaatcca aactggagga
     2641 aaccttgaac ttgaagaagt ggctaatcgc gatccccctc accctggccc tggcgctggg
     2701 tgtggccgcc tgcggaagca gcgacagcga cagtggcagc agcgggtcca gcggcgacgc
     2761 ggtctccggc gagatcgccg gcgccggcgc cagctcacag gaagccgcca tggccgcctg
     2821 ggtcgccggc ttccaggaga acaatcctga cgccaacatc gcctacgacc cggtcggttc
     2881 cggcggcggc cgcgagcagt tcatcgccgg tggcaccgac ttcggcggca ccgacacccc
     2941 gatggacgag accgaactgg ccgatgtcgc caagcgctgc aagccgggcg agtacatcga
     3001 gatcccggtc tacatctcgc cgatcgcggt tccgtacaac gtcgaggggg tcgatgacct
     3061 gaacctctcg gccagcaccc tggcccggat cttcgctggt gacatcacca agtggaacga
     3121 tcaggcgatc gcggcggaga accc
//
LOCUS       DAOQFI010000143         3140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15206_length_3140_cov_3.147488, whole genome shotgun sequence.
ACCESSION   DAOQFI010000143 DAOQFI010000000
VERSION     DAOQFI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15206_length_3140_cov_3.147488"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..961
                     /locus_tag="PLE93_03500"
     CDS             134..961
                     /locus_tag="PLE93_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86145.1"
                     /translation="MKTRLVALLAILVTGLFVAVGPAPAQSAVPGIKQTVAYKRLKNY
                     VGFLQSKRNVPVKTTRRKTYKTTLSNRRNNANTKVLSLYSRTLLKISREDDAKQRRDI
                     KRIRQNQKAQVQALNARQANRLAALQAKENAASARIYASYNLKIDPLANKRDRLRRQL
                     ARTTKPAKRSALTKQINKLQVKINGLVDDRTTDLNGLANRFNLKAQNIRNQFGQLIAN
                     VKANAQKQIQQERNAWRQTFRTQVQAAKTRRDAQKDMVGSQAERGFSFISQMPPAGG"
     gene            complement(1101..1465)
                     /gene="ssrA"
                     /locus_tag="PLE93_03505"
     tmRNA           complement(1101..1465)
                     /gene="ssrA"
                     /locus_tag="PLE93_03505"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1559..2911
                     /locus_tag="PLE93_03510"
     CDS             1559..2911
                     /locus_tag="PLE93_03510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH86146.1"
                     /translation="MSIHGFDVVVLGAGPAGEVFAGQSAEAGFRVALVEQHLVAGECS
                     YYACMPSKALLYPGEVLDEARRAPGVREAVTGGLDVSAVLERRDEVIHHLDDSSQLAW
                     LRERGIELFRGSGVIAGEREVRVGGDVLVADRAVAIATGSSASIPPIPGLPEAPIWNN
                     RQGTTASTAPESLIILGGGPVGCELAQAWSSLGSEVTIFEPEERVLVHEEPFAGDLVE
                     QALWDRHGVTMVTGTADSGITVSAGGKEYTAAELMVATGRRPRTDRIGLESIGVDGGA
                     ELETDDCYRVKGHDWLFAVGDVNGRAPLTHMGKYQAWVGVRALLGHEPENRAELIGSP
                     RVTFTNPQVAAVGKTLAEAEAEGIDAVAIDADTGSTAGASFLGRGVDGKCRLVVDQAT
                     GLVVGATFVGFEVAEWLQAATVAIVGKVSMDELRHAVAPFPTRSEIWLRFSEEWEARR
                     "
     gene            2921..>3140
                     /locus_tag="PLE93_03515"
     CDS             2921..>3140
                     /locus_tag="PLE93_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNH86147.1"
                     /translation="MKILAFSDLHRDLDQAARLTEMSRQADVVIGAGDFASIHQGLEE
                     TIDALAGIETPTVLVPGNNETLDALKEAT"
BASE COUNT          586 a         1034 c         1013 g          507 t
ORIGIN      
        1 cccccgggac aagaccactt ccccccgcgc caacccctga gtccggcgaa gacggcggcc
       61 gccggtgccg atgttttcat tccttttcgg attttcgcaa ctgatgcctg cggcaggtgt
      121 tagtttcgac accatgaaaa ctcgcctcgt cgcactcctc gcaatcctcg tcaccggact
      181 gttcgtagcg gtcggacccg cgccggccca gagcgccgtc cccggcatca agcagaccgt
      241 cgcctacaaa cggctgaaga actacgtcgg ctttctccag tcgaagcgca acgtcccggt
      301 caagacaact cgacgcaaga cctacaagac cacgctttcg aaccggcgca acaacgcgaa
      361 cacgaaggtg ctgagtctct actcgcggac cctgctcaag atctcccgcg aagacgacgc
      421 gaaacaacgc cgtgacatca agcggatccg ccagaaccag aaggcccagg tccaggccct
      481 gaacgcccgg caggcaaacc ggcttgctgc gctccaggcg aaggagaacg ccgcctcggc
      541 aaggatctac gcctcctaca acctgaagat cgacccgctg gcaaacaagc gggaccggtt
      601 gcgcaggcag ctcgcccgca cgaccaaacc ggccaaacga tcggccctga ccaagcagat
      661 caacaagctc caggtcaaga tcaacggtct ggtcgatgac cgcaccaccg acctcaacgg
      721 tctcgccaac cgcttcaacc tgaaggcgca gaacatccgc aaccagttcg gtcagctgat
      781 cgccaacgtg aaggcgaatg cccagaagca gatccagcag gaacggaatg cgtggcgcca
      841 gaccttccgc acccaggtcc aggcggcgaa gacccgtcgc gatgcccaga aggacatggt
      901 cggctcccag gccgaacggg gcttcagctt catctcgcag atgccgccgg cgggtggctg
      961 aacaccgtca gccgcccgat ccgggcagaa atcagcctcg gcgaaagggc ccgctcacca
     1021 ggcgggcctt tttctttcgt tcgcagacag tggagaccag agtgcaaggt cgccactgga
     1081 aaacccgccg gagcgggttt cagtggagac ggcgggaatt gaacccgcgt ccgcagtcgc
     1141 gtgtcgaaag gcgtctacag gcttagccgg cgctttgttc tcgccacccg gggtccgcgc
     1201 cggcgggcac tgcggatggc aaagcctctt gaatgtcccc ctccggccaa ggcgctcctt
     1261 cggggtaagc ccgtttctga agccggattt ccaccccacg ggccgagggc gggccgactc
     1321 tcgttaccta ggtatcagct aggcggcgag ggcgtacgag tgttcgtcgc cgtatatggt
     1381 tttttgccga ttttttacga ggccatcgac atcctcgacc tgcaaccctc cgcacggtcc
     1441 gacaacgtcg aagcctgtcg tccccttgga tggtgctcct ccattgtacg gtgtctctct
     1501 ggccgggtca aagcagcaac cggccaggca agagcccaga tcgaccagcg gaggcagttt
     1561 gagcatccac ggatttgacg tcgtcgtcct cggggcaggg ccagccggag aggtctttgc
     1621 cggtcaatcg gccgaggccg ggttcagggt cgccctggtc gaacagcatc tggttgccgg
     1681 cgagtgctcc tattacgcat gcatgccgtc caaggcgctg ctctaccccg gtgaggtcct
     1741 ggacgaggcc aggcgcgccc cgggggtcag ggaggcggtc accggcggtc tggacgtctc
     1801 cgccgttctg gaacgccgcg acgaagtgat tcaccacctt gacgattcca gccagctggc
     1861 ctggctcagg gagcgcggga tcgaactctt tcgcggctcc ggggtgatcg ccggtgaacg
     1921 cgaggtcagg gtcggaggcg atgtcctggt ggccgaccgg gcggtcgcca tcgccaccgg
     1981 ctcctcggcg tcgatcccgc cgattcccgg tctgcccgag gccccgatct ggaacaaccg
     2041 gcagggcacg acggccagca ccgcacccga atccctgatc atcctcgggg gcggtccggt
     2101 cggctgcgag ctcgcccagg cctggtccag cctcggttcc gaggtaacca tcttcgagcc
     2161 ggaagaacgg gtgctcgtcc atgaggaacc ctttgccggc gatctggtcg aacaggctct
     2221 ctgggaccgt cacggggtga ccatggtcac cggaacggcg gattcgggaa tcaccgtttc
     2281 cgccgggggc aaggagtaca cggcggccga actaatggtc gcgaccggcc gccggccccg
     2341 gaccgaccgg atcggtctgg aatcgatcgg cgtcgacggc ggcgcagagc tggaaaccga
     2401 cgactgttac cgggtgaagg ggcatgactg gctgtttgcc gtcggtgacg tgaacggccg
     2461 ggcgccgctc acccacatgg gcaagtatca ggcctgggtc ggggtccggg ccctgctcgg
     2521 gcacgagccg gaaaaccggg ccgagttgat cggctctccc cgggtgacct tcaccaatcc
     2581 ccaggtggcg gcggtcggca agaccctcgc cgaggccgag gcggagggaa tcgatgcggt
     2641 ggcgatcgat gccgataccg gctcgacggc cggggcctcg ttcctcggca ggggagtcga
     2701 cggcaagtgc cggctggtgg tcgaccaggc gaccggactg gtggtcggag ccaccttcgt
     2761 cggattcgag gttgccgagt ggctgcaggc ggcgaccgtc gcgatcgtcg gcaaggtttc
     2821 aatggacgaa ctgcggcacg ccgtcgctcc cttccccacc cgaagcgaaa tctggttgag
     2881 gttcagcgag gagtgggaag cacggcggta aggttggcac atgaagatcc tcgctttcag
     2941 tgacctgcac cgtgacctgg accaggcggc ccggttgacc gagatgtccc ggcaggccga
     3001 cgtggtgatc ggcgccggcg actttgcctc gatccatcag ggcctggagg agacgatcga
     3061 tgccctcgcc gggatcgaga ccccgaccgt gctggttccg ggtaacaacg agacgctcga
     3121 tgccctgaag gaagccaccg
//
LOCUS       DAOQFI010000144         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15298_length_3127_cov_3.653971, whole genome shotgun sequence.
ACCESSION   DAOQFI010000144 DAOQFI010000000
VERSION     DAOQFI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15298_length_3127_cov_3.653971"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLE93_03520"
     CDS             complement(<1..464)
                     /locus_tag="PLE93_03520"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNH86148.1"
                     /translation="MGRLTCLTAAVFATLALSSASLACAEGDGGNPPERAATGKVSVG
                     GPDAAGASAGHGKLRATRVGFFNEPVEIRHAPGFPNLMFVVEQPGRIMVIRRGRKLAR
                     PFLDINRLVSNDGGERGLLSVAFPPDYRKSKRFYVYYVDNAGNIRVAEFRRRT"
     gene            complement(536..1231)
                     /locus_tag="PLE93_03525"
     CDS             complement(536..1231)
                     /locus_tag="PLE93_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86149.1"
                     /translation="MPRSIVTLGLVLGALAGALLVSGCGGGDDDGQFAGQPTGDFPVK
                     VLDATFKPLQTVADTYDLKIAVENTGEKTIPGLNTVINLPGEGSTLAFAYGDKQQGLA
                     MNQRPVWVLEEGWPKLAGTTGRGGATTADRRTFEFGELKPDQTATMIWRVVAVRPGNY
                     RLEYQISAGLGPDTRAVDNSGHPPTGILPVRISDFARLTEINEKGQVVPVSRAEQARV
                     EAQQQSADTELAP"
     gene            1368..2174
                     /locus_tag="PLE93_03530"
     CDS             1368..2174
                     /locus_tag="PLE93_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH86150.1"
                     /translation="MVKATANKAIEPAKEFFTETGEMMILTGRTVLSAIKPPYPYGNE
                     FIGQFLFALQLCWFPMLISCVAFGFGAPGLQAANFLSLFGALDRLGGFFVLASIREFA
                     PFVTAVVVAGVAGTAITADLGARKIREELDALQVLGVDPIKNLVVPRFLALMLITGML
                     DIYALLFGIAGGIGAELLYSQPLGGFFASLFSNASTTELAASIIKCTLFGAIIAIVCC
                     YKGMTATGGAAGVGRAVNEAVVTALLGVFIFNYLFTQTMLATNPDIQTIK"
     gene            2174..2974
                     /locus_tag="PLE93_03535"
     CDS             2174..2974
                     /locus_tag="PLE93_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH86151.1"
                     /translation="MATSWLQVPREWLESMGEIVRFCGAVMGLVFSGRVFKYFGEVLN
                     QAGILILGSTTVIWGLVFVLGLQCGIEGAYFNRSVGAPAYAGVFAAWCDLREVIPYAF
                     GYMMSAKVGTGIVAELGAMRISDEIDALEVMGVPPVTFLAATRLLGAWITLPFMYIAA
                     IGVGFLASYIAVVEQIGDVSSGGYQLIFWMFQNPPDVLFSLIKAMTMSTAIVLVGCYY
                     GYTASGGPVGVGTATAKSMVLNIVLVHIIGMLGTLVFWGANPRAPIGG"
BASE COUNT          517 a         1032 c          987 g          591 t
ORIGIN      
        1 gtccggcgcc ggaactcggc gacccggatg ttgccggcgt tgtctacgta atagacgtaa
       61 aagcgtttgc tcttcctgta atcgggcggg aaggcaaccg aaagcagacc tcgctcgccg
      121 ccgtcattgc tgaccagccg gttgatgtcg agaaagggcc gggccagttt cctgccgcgt
      181 cggatcacca tgatccggcc cggctgctcg accacgaaca tcaggttcgg gaaaccgggg
      241 gcgtgccgga tttcaaccgg ttcgttgaag aagccgaccc gggttgcccg aagtttgccg
      301 tgcccggccg aggcgccggc ggcatccggc ccgccgaccg aaaccttgcc ggtggcggcg
      361 cgctccggcg ggttaccgcc gtcaccttcg gcgcaggcaa gcgaggccga cgagagcgcg
      421 agagtggcga aaacggctgc ggtgaggcaa gtaagtcttc ccatgatctt catccgtacc
      481 cgaaaaccga ccgggcaagc caagttgcga acggccgggg gacgactttc tcgaatcagg
      541 gagcgagttc ggtgtcggcc gactgctgct gggcttcgac cctggcctgc tcggcgcggc
      601 tgaccggaac gacctggccc ttctcgttga tctcggtgag acgggcgaaa tcgctgatcc
      661 tgaccggcag gatcccggtc ggcggatggc cgctgttgtc gaccgcccgc gtgtccgggc
      721 cgaggccggc cgagatctgg tactcgagcc ggtaattgcc gggccgcacc gcgactaccc
      781 gccagatcat ggtggcggtc tgatcgggct tcagctctcc gaattcgaag gttcgccggt
      841 cggcggtggt ggctccgccc cggccggtgg tcccggccag cttcggccag ccttcctcga
      901 gcacccagac cggacgctgg ttcatcgcga gaccctgctg cttgtcgccg taggcaaagg
      961 ccagggtcga accctcgccg ggcaggttga tcacggtgtt gagaccgggg atcgtcttct
     1021 cgccggtgtt ctcgaccgcg atcttcaggt cgtaggtatc ggcgaccgtc tgcaggggct
     1081 tgaaggtcgc gtccagaacc ttgaccggga agtcgccggt cggctggccg gcaaactggc
     1141 cgtcatcgtc gccaccgccg cagccggaaa cgagcagggc gccggcaagc gcaccaagga
     1201 ccaatccgag ggtgacgatg gatctgggca tggatctgcg gagtgctccc ctcaagaggg
     1261 gcgcaacata ccacgcgtgt ggcgacggtc acacacgccg ggggtatcgc atagactgcg
     1321 cgccacggcg ggtcacgtgt tgtccagcgg ggcctggccc gggagagatg gtcaaagcaa
     1381 ccgcaaacaa ggcgatcgag cccgcgaagg aattcttcac cgaaaccggt gaaatgatga
     1441 tcctgaccgg acgcacggtc ctgtccgcga tcaagcctcc gtacccctac ggcaacgagt
     1501 tcatcggcca gttcctcttc gcccttcagc tttgctggtt cccgatgctg atctcctgtg
     1561 tcgcctttgg cttcggcgcc ccggggctcc aggcagccaa cttcctttcg ctcttcggag
     1621 ctctcgaccg cctcggcggt ttcttcgttc tggcctcgat tcgcgagttc gcaccgttcg
     1681 tgaccgccgt cgtggtcgcc ggtgtggccg gcaccgcgat caccgccgac ctcggtgccc
     1741 gcaagatccg cgaggaactc gacgccctcc aggtgctcgg cgtcgatccg atcaagaacc
     1801 tcgtcgtgcc ccgcttcctc gccctgatgc tgatcaccgg catgcttgac atctacgccc
     1861 tgctcttcgg catcgccggc ggcatcggcg ccgagctgct ctactcgcag ccgctgggcg
     1921 gcttcttcgc ttccctcttc tcgaacgcct cgaccacaga gctcgccgcc tcgatcatca
     1981 agtgcaccct gttcggcgca atcatcgcga tcgtctgctg ctacaaggga atgaccgcga
     2041 ccggcggtgc ggccggcgtc ggccgggccg tcaacgaggc ggttgtcacc gccctgctgg
     2101 gggtattcat cttcaactac ctgttcaccc agaccatgct ggcgaccaac cccgacatcc
     2161 agacgatcaa gtaatggcta cgagctggct acaggtcccc cgcgagtggc tcgaatcaat
     2221 gggcgagatc gtccgtttct gcggcgcggt catggggctg gtcttctccg gccgggtctt
     2281 caagtatttc ggcgaggtcc tcaaccaggc cggcatcctg atcctcggat cgaccacggt
     2341 catctggggc ctggtcttcg tgctcggcct gcaatgcgga atcgagggcg cctacttcaa
     2401 ccgctcggtc ggcgcacccg cctacgccgg cgtcttcgcc gcctggtgcg acctgcgtga
     2461 ggtcatcccc tacgccttcg gctacatgat gtcggccaag gtcggcaccg gcatcgtggc
     2521 cgagctcggc gcgatgcgga tctcggatga gatcgacgcc ctcgaagtca tgggcgtacc
     2581 gccggtcacc ttcctcgccg ccacccgcct gctcggcgca tggatcaccc tccctttcat
     2641 gtacatcgcc gcgatcggcg tcggcttcct cgcctcctac atcgcggtgg tcgaacagat
     2701 cggcgacgtt tcgtccggtg gctaccagct gatcttctgg atgttccaga acccgcccga
     2761 cgtcttattc agcctgatca aggcaatgac gatgtccacg gccatcgttc tggtcggctg
     2821 ctattacggg tacacggcat cgggcggccc ggtcggggtg ggtaccgcca ccgcgaaatc
     2881 aatggttctg aacatcgtgc tggtacacat catcggtatg ttgggaacgc tggtcttctg
     2941 gggtgcgaac ccgagagcac cgattggagg ctgagaggtt gagcgaaaca gaagaaacac
     3001 ctgacgcgca gggttcggag gaaactccga tcaactctcc cgaggtcgaa gccgcggtcg
     3061 aaagcaccgg tgatggcgaa gtggccgccg agatgaaagc agctcgaact ttttccgcat
     3121 ccgccct
//
LOCUS       DAOQFI010000145         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15429_length_3113_cov_3.670046, whole genome shotgun sequence.
ACCESSION   DAOQFI010000145 DAOQFI010000000
VERSION     DAOQFI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15429_length_3113_cov_3.670046"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PLE93_03540"
     CDS             <1..1149
                     /locus_tag="PLE93_03540"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH86152.1"
                     /translation="RMKDLISRRSLVLTAARFVVLDEADEMLDLGFIEDVERILRMCP
                     SGRQTALFSATMPPPIKRLADTFMYDPVTISITPKTLTVDAIEQAFVEVPGRAKVDKL
                     IEVVKEEDPEQAILFCRTKIGASRLERDLKNRGLDVKALHGDLSQGVRDGVMLAFKNH
                     RCKLLVATDIAARGLDVDHVTHVINYDIPNSSETYVHRIGRTGRAGRTGRAITFVTPD
                     QRRDLEQIKKDVKASISEWEKPEDRIEHARPPRRKRRGEQPAPLDDQPAVAVEEVEEF
                     DEVVEVPLEGPGGDQPETASDDIPDAREGAVKLFVNRGSRSDITEEDLRWALREGAVL
                     SDDQISDVRVLERFSFVEVDGDVAEKTVEFLDGTKLKGSGIRVEIARS"
     gene            complement(1166..1528)
                     /locus_tag="PLE93_03545"
     CDS             complement(1166..1528)
                     /locus_tag="PLE93_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH86153.1"
                     /translation="MGRILVVEDSSAIRLLIRRRLEMAGHQVEEAANGLEALDQLGST
                     AEPDLIVLDESMPVFDDAGLLTRLREQWSELPVIVVSASPASESDPIMHSADARLAKP
                     INFERLFEVIDRLTSESP"
     gene            complement(1528..2589)
                     /locus_tag="PLE93_03550"
     CDS             complement(1528..2589)
                     /locus_tag="PLE93_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH86154.1"
                     /translation="MGPEPRKPSVAYLEQVLGVLPMVIAAIDDAGRIIWANAPTSRIA
                     PLLLETRSIREALAGIAHEQKLDRLLIRHEVITCPGRTGGPDLHWMVWTEPLAGGEWV
                     LMVWEADWSEEMNERRTAFTMAASHELRTPLTALLGFAEILEMDTDNLKPAQIEAVQM
                     ISQTALHLTKLVDDVFELTSNSFGELRLNLERVDLGEIARVLVEAHLQEAANRGDTLT
                     LEVQEDIPPVDADPARVRQMFANLVRNAMVHNPSGTRIVMKLWTDDDAVAVSVSDDGR
                     GLPFSNPKQALSSFSQGDGGSGDRTGAGIGLTIAKRLIELHRGSIEVQSTFGEGSTFT
                     LRFPIDRAKAISPGEPGPA"
     gene            complement(2593..>3113)
                     /locus_tag="PLE93_03555"
     CDS             complement(2593..>3113)
                     /locus_tag="PLE93_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH86155.1"
                     /translation="RSSFGELRLDLHEIDLNQVIAVVISSLEPGISDRGQTLTLDAEE
                     LPVIEADESRMIQMISNLVSNASVHNAAGVKIRVKARRSGNEIAITVADDGVGLPFDD
                     PEAVFLSFRRGEAASDGDRTGSGIGVPLTKLLVELHRGRITVETGRGEGTSFTLWLPV
                     DREAALAEGTGL"
BASE COUNT          552 a         1008 c          994 g          559 t
ORIGIN      
        1 cggatgaagg acctgatttc ccgccggtcc ctggtcctca ccgcggcccg cttcgtggtg
       61 cttgacgagg ccgacgagat gctcgacctc ggtttcatcg aggacgtcga gcggatcctc
      121 cggatgtgcc cgagcggccg tcagaccgcc ctattctcgg ccaccatgcc gccgccgatc
      181 aagcggctgg ccgacacctt catgtacgac ccggtgacca tctcgatcac cccgaagacc
      241 ctcaccgtcg acgcaatcga gcaggccttc gtcgaggtgc cggggcgggc caaggtcgac
      301 aagctgattg aggttgtcaa agaagaggat cccgagcagg cgatcctctt ctgccgcacc
      361 aagatcgggg cttcgcggct ggagcgtgac ctgaagaacc gcggcctgga cgtcaaggcg
      421 ctgcacggcg acctcagcca gggagtccgc gacggagtga tgctggcctt caagaaccat
      481 cgctgcaagc ttctggtcgc gaccgacatc gcggcccggg ggctggatgt cgaccacgtc
      541 acgcacgtga tcaactacga catcccgaac agttccgaga cctacgttca ccggatcggg
      601 cgtaccggcc gggcggggcg taccggccgg gcgatcacct tcgtcacccc ggaccagcga
      661 cgggacctgg agcagatcaa gaaggacgtg aaggcgtcga tctccgaatg ggagaagccc
      721 gaggaccgga tcgagcacgc ccggccgccc cgccgcaagc gtcgcggcga gcagccggct
      781 ccgcttgacg accaaccggc agtggcggtc gaggaggtcg aggaattcga cgaggtggtc
      841 gaggttccgc tcgagggtcc aggcggcgac cagccggaaa ccgcttccga tgacatcccg
      901 gacgcgaggg agggtgcggt caagctcttc gtcaaccggg gttcccgctc ggatatcacc
      961 gaggaagacc tgcgctgggc cctgagggaa ggcgctgtcc tcagtgatga ccagatctcc
     1021 gacgtgcggg tgctggaacg cttttccttc gtcgaggtgg acggcgacgt ggccgagaag
     1081 acggtcgagt tcctcgacgg caccaagctc aagggttcag gcatccgggt cgagatcgcc
     1141 cgcagttagg accacctaca gcggctcacg gagattcgct ggtcaggcga tcgattactt
     1201 cgaaaagccg ttcgaaattg attggcttgg ccagccgggc atcggccgaa tgcatgatcg
     1261 gatcgctttc cgaggcggga ctcgcgctga cgacgatcac cggcagctcg gaccactgtt
     1321 ccctgagccg ggtgagcaaa ccggcatcat cgaacaccgg catgctctcg tcaagcacga
     1381 tcaggtccgg ctctgcggtg gatccgagct gatcgagcgc ctcaaggccg ttcgcggcct
     1441 cttcgacctg atgtccggcc atctccagtc ttcgcctgat cagaagccgg atcgccgatg
     1501 aatcctcgac caccagaatc cggcccatca ggccggtccc ggctcgcccg gcgaaatcgc
     1561 cttcgctcga tcgatcggga aacgcagggt gaaggtgctg ccttcgccga aagtcgactg
     1621 gacctcgatc gacccgcggt gcagttcgat caggcgcttg gcgatggtca ggccgatccc
     1681 ggcaccggtc cggtctccgc tgccgccgtc tccctggctg aagcttgaga gagcctgttt
     1741 cgggttgctg aaggggagcc cccggccatc atcggaaacc gagaccgcta ccgcgtcgtc
     1801 atcggtccac agcttcatca cgatcctggt tccggagggg ttatggacca tcgcgttacg
     1861 cacgaggttg gcgaacatct gccggacccg cgccgggtcg gcgtcaaccg ggggaatatc
     1921 ttcctggact tccagggtca gggtgtcgcc ccggttcgca gcttcctgca ggtgcgcctc
     1981 gaccagaacc cgggcgatct ctccgaggtc gacccgctcc aggttgagcc tgagttcacc
     2041 gaagctgttg ctggtcagtt cgaagacgtc atcgaccagc ttggtcagat gaagggcggt
     2101 ctgggaaatc atctggaccg cttcgatctg ggcgggcttc aggttgtcag tatccatctc
     2161 gaggatttcg gcgaacccca gcagagcggt cagcggcgtc ctgagctcat gcgaggccgc
     2221 catggtgaac gccgtgcgtc gctcgttcat ctcctctgac cagtcggcct cccagaccat
     2281 caggacccac tcgcccccgg ccagtggctc ggtccagacc atccagtgga ggtcgggtcc
     2341 acccgtccgg ccggggcagg tgatcacttc atgccggatc agcaggcggt cgagcttctg
     2401 ctcgtgggca atgcccgcca gggcttcccg aatcgaccgt gtctccagca ggaggggtgc
     2461 gattcgtgaa gtcggagcgt tggcccagat gatccggccg gcatcgtcga tcgcggcgat
     2521 caccatcggc aggactccga gcacctgctc caggtatgcg accgaaggct tccggggttc
     2581 cggacccatc ggtcagagtc cggttccctc ggccagggcc gcttcacggt cgaccggcag
     2641 ccacagcgtg aacgaggtgc cttcgccccg gccggtctcg accgtgatcc ggcctcgatg
     2701 aagttcgacc agaagcttgg tcaggggaac tccgatcccg gagccggttc ggtctccgtc
     2761 acttgcggct tcgccccggc ggaaactgag aaaaacagcc tcggggtcat cgaatggaag
     2821 gccgacgccg tcatcggcca cggtgatcgc gatctcgttc ccggaccgtc gggccttgac
     2881 ccggattttc accccggccg cattgtgaac cgatgcgttg ctgaccagat tcgaaatcat
     2941 ctggatcatc cggctctcgt cggcttcgat caccgggagt tcttcggcat ccagagttag
     3001 cgtctgtccg cggtcgctga ttccgggctc gagtgagctg atgactaccg cgattacctg
     3061 gttgaggtcg atctcatgca gatccagacg cagctctccg aaactgctcc tgg
//
LOCUS       DAOQFI010000146         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15455_length_3110_cov_3.068740, whole genome shotgun sequence.
ACCESSION   DAOQFI010000146 DAOQFI010000000
VERSION     DAOQFI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15455_length_3110_cov_3.068740"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLE93_03560"
     CDS             <1..806
                     /locus_tag="PLE93_03560"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNH86156.1"
                     /translation="DVSLRSSSAGDVAVIDRDSGEVIGTVETGRAFSTIHPGAVYMHL
                     GASWEVERLDPYEGEAIVHRFDEEWYTRPKTETEIFINRVLEEKEVCGVKLSFGEISV
                     TDQVMAYQRILSADGEAFDMVDLDLPEQEFATRGLWYEVPEDFIGGMALNEMLGALHA
                     TEHGQIAVLPLIAMCDRWDIGGLSTNLHMQTGTPTIFIYDGHPGGIGLTKRAFELFPR
                     LVGDAHRLIAECPCDSGCPSCVQSPKCGNLNEPLFKAGSISLLEAMASA"
     gene            849..1646
                     /locus_tag="PLE93_03565"
     CDS             849..1646
                     /locus_tag="PLE93_03565"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNH86157.1"
                     /translation="MRTIEGSSGRIVVHEWIPVGEPTYVVLLAHGYAEHALRHAGLAE
                     KFEADGAAVYAPDHRGHGRSEGEPALVDEAQPMIDDLAAVYDLAREEHPGLPILLFGH
                     SMGGLLATLFIQEAERPVDAVVLSSPLLGGNPALVALLDLEEIPEIPIDPTVISRDEN
                     EQQAYLDDPMIYHGPFRRETLEGLVAGLDRARSGDGFGELPLLWIHGEGDMLVPYDLT
                     AETMEILSGPGTEAKSYPEARHELFHETNREDVYADLFDFVNRNLET"
     gene            complement(1746..1818)
                     /locus_tag="PLE93_03570"
     tRNA            complement(1746..1818)
                     /locus_tag="PLE93_03570"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1783..1785),aa:Phe,seq:gaa)
     gene            complement(1970..2890)
                     /locus_tag="PLE93_03575"
     CDS             complement(1970..2890)
                     /locus_tag="PLE93_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86158.1"
                     /translation="MRNVAGFFTVPKNTGLDFPPVTVDLDVVQIQGEIALTKDGSGHF
                     DEATGAMNMDLSLSLTLGVDDLEALSQQVGIPLGTGALGCKISPLEVSFSTAKGWPHA
                     GSSFTDKATPADGALAGAWRYKPAITAVEGDQSVCNIIGGFLKDVGGIWLGNSTTPLA
                     DMPAATDPMPAKANCTELGKTGTFPNCVDPVCPEGQLGTYPDCHDPAPFAVSKVALSP
                     TKANIKKNKTVKLTLKVTNSGEVSGSATVSLKSSNKQVTLPKSIKVTVPAGKTASKTI
                     TVKAAKKAKGKATITATASGKKGTATLTVK"
     gene            2973..>3110
                     /locus_tag="PLE93_03580"
     CDS             2973..>3110
                     /locus_tag="PLE93_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNH86159.1"
                     /translation="MSSSLSSGRVSPKATAGAAALTLGALGVVFGDIGTSPLYAFQTV
                     FQ"
BASE COUNT          524 a          992 c         1036 g          558 t
ORIGIN      
        1 ccgacgtttc gctccgctcc tcctcggccg gggacgtcgc ggtgatcgac cgtgattccg
       61 gcgaggtgat cggcacggtg gagaccggtc gcgccttcag caccatccat cccggcgcgg
      121 tctacatgca cctcggcgct tcctgggagg tggagcggct cgatccgtac gagggcgagg
      181 cgatcgtcca ccgtttcgat gaggagtggt acacccggcc gaagaccgaa accgagatct
      241 tcatcaaccg ggtgctcgag gagaaggaag tctgcggggt gaagctgagc tttggcgaga
      301 tctcggtcac cgaccaggtg atggcctacc agcgcatcct tagcgctgac ggcgaggcgt
      361 ttgacatggt tgatctcgac ctgcccgagc aggagttcgc gacccggggc ctctggtacg
      421 aggtacctga ggatttcatc ggcggcatgg ccctgaacga gatgctcggc gccctccacg
      481 cgaccgagca cggccagatc gcggtgctgc cgctgatcgc gatgtgtgac cgctgggaca
      541 tcggcggtct ttcgaccaac ctccacatgc agaccggcac cccgaccatc ttcatctatg
      601 acggccaccc cggcggcatc gggctgacca agcgcgcctt cgagctcttc ccccgcctgg
      661 tcggcgatgc ccaccgcctg atcgcggagt gcccctgcga ttccggttgc ccctcttgcg
      721 ttcagagccc caaatgcggg aacctgaacg agccactgtt caaggcgggc tcgatcagcc
      781 tgctggaggc gatggcttcg gcctgattcg gccacccaga aggcaccagc aaagtcaaag
      841 gagagcggat gcgcaccatc gagggaagca gcggcaggat cgtcgtccac gagtggattc
      901 cggtcgggga gccgacatat gtggtgctgc tcgcccatgg ttacgccgaa cacgcgctca
      961 ggcatgccgg cctggccgag aagttcgagg cggacggggc ggcggtctac gcgccggatc
     1021 atcgcggcca cgggcgctcg gagggcgagc cggccctggt cgatgaagcc cagcccatga
     1081 tcgacgacct cgccgcggtc tatgacctgg ctcgcgagga acaccccggc ctgccgatcc
     1141 tgctcttcgg ccattcgatg gggggtctgc tcgccaccct gttcatccag gaggcggaac
     1201 gcccggttga tgcggtcgtc ctttcgagcc cgctgctggg cggcaatccc gcactggtcg
     1261 ccctgctcga cctcgaggag atcccggaga tcccgatcga cccgacggtg atcagccgtg
     1321 acgagaacga gcagcaggcc tatctcgacg acccgatgat ctaccacggc cccttccgtc
     1381 gcgaaacgct ggaaggcctg gtcgccgggc tggaccgggc ccggagcggt gacggtttcg
     1441 gcgagttgcc gctgctctgg attcacggcg agggggacat gctcgtcccc tacgacctga
     1501 ccgccgaaac gatggagatc ctgagcggcc cgggcaccga agcgaagtca tacccggaag
     1561 cccgccacga gctattccac gaaaccaacc gcgaagacgt ctacgccgac ctcttcgact
     1621 tcgtcaaccg aaacctcgag acctagcagg gcagagcccg acccaacacc attcatttct
     1681 tccagtgggt cgggcgggtt gctcgtggag ggcgggtcgg accccgcccg actactcgtg
     1741 acatatggcg agggccggga tcgaaccggc gacaccacga ttttcagtcg tgtgctctac
     1801 caactgagct acctcgccaa ggaaggacag gctatcggga atcgccggtt cagcgacgct
     1861 tggcgcgggg ccgaaacggg tccgggaaac ccgggcaaag gaaagggccc cggatcactc
     1921 cggggccctc gcttcactcg gtccggcctg gccggaagaa gtgtcctcgc tacttgactg
     1981 tcagggtggc ggtgcccttc ttgccactgg cggtggcggt gatggtcgcc ttgcccttgg
     2041 ccttcttggc agccttgacc gtgatggtct tgctggccgt cttgccggcc ggaacggtca
     2101 ccttgatcga cttcggcagg gtgacctgct tgttgctcga cttcagtgaa accgtcgcgc
     2161 taccggaaac ctctccggag ttggtgacct tgagggtcag cttgacggtc ttgttcttct
     2221 tgatattggc cttggtcggc gaaagcgcga ccttgctcac cgcgaaggga gccggatcgt
     2281 ggcagtcggg gtaggtcccc agctggcctt cggggcagac cgggtcgaca cagttcggga
     2341 aggtacccgt cttgccgagc tcggtgcagt tggcctttgc cggcatcggg tccgtcgcgg
     2401 caggcatgtc ggcgagcggc gtggtgctgt tgccgagcca gatgccaccg acgtccttga
     2461 ggaagccacc gatgatgttg cagacggact ggtcgccttc aacagcggtg atcgccggct
     2521 tgtagcgcca ggcacctgcc agggcgccgt cggccggagt tgccttgtcg gtgaagctgc
     2581 tgcccgcatg cggccagccc ttcgcggtcg agaaggagac ttccagcggc gagatcttgc
     2641 agccgagcgc gccggtgccc agcgggatgc cgacctgctg cgagagagct tcgaggtcgt
     2701 caacgccgag ggtcagcgag aggctgagat ccatgttcat cgcaccggtg gcctcgtcga
     2761 aatgaccgga gccgtccttg gtcagggcga tttcaccctg gatctggacg acgtcgaggt
     2821 cgacggtgac cggcgggaag tccaggccgg tgttcttcgg cacggtgaag aaaccggcaa
     2881 catttcgcaa acaatcggtt tctggattgg cggttgcggg tagagggggc gatcgagccg
     2941 gaaaccggcg cgaggcgtag gctccccggc tgatgtcttc gtccctttcc tcaggcaggg
     3001 tttcaccgaa agccacggcc ggtgcggccg cgctgacgct cggcgcactc ggagtcgtct
     3061 tcggggacat cggcaccagt ccgctctacg ccttccagac cgtcttccag
//
LOCUS       DAOQFI010000147         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15516_length_3102_cov_4.090253, whole genome shotgun sequence.
ACCESSION   DAOQFI010000147 DAOQFI010000000
VERSION     DAOQFI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15516_length_3102_cov_4.090253"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1278
                     /gene="moeB"
                     /locus_tag="PLE93_03585"
     CDS             106..1278
                     /gene="moeB"
                     /locus_tag="PLE93_03585"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNH86160.1"
                     /translation="MAPEATEQQTIEEITPQEAFAELDGGGVKLIDTREPYEYEESHI
                     EGGELIPPGVIADRIEAAVPDKDQRIILQCRSGARSLVAAEALAGLGYTNLANMSGGI
                     LEWEAQGLPVESNVALTPEQRDRYSRHILLPEVGVEGQIKLLEAKVLLLGAGGLGAPT
                     ALYLAAAGIGTIGIVDDDVVDASNLQRQVIHNTSRVGQPKSASARQTIEELNPDVEVI
                     EYNFRLDAGNILEVIEDYDIIVDGADNFPTRYLLNDASVRLKKPVVSASILSFDGQIS
                     TFIPYEGPCYRCLYPTPPPPELAPSCSANGVLGAMAGQMGLLQANEVLKLVLGIGEPL
                     VGRLLLYEALGTRFTELKVRRDPECPICGPNAPKVPESEMGQFPDYEAFCGGHVGS"
     gene            1322..1597
                     /locus_tag="PLE93_03590"
     CDS             1322..1597
                     /locus_tag="PLE93_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HNH86161.1"
                     /translation="MPSVKIPPVLRKHTGDQTSVDVAGSNVGEVLNALVAEYPETKDQ
                     LFSGEGELNRFVNVYLNDEDVRVLDGLETGVSDTDTVMILPAMAGGE"
     gene            complement(1694..1972)
                     /locus_tag="PLE93_03595"
     CDS             complement(1694..1972)
                     /locus_tag="PLE93_03595"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNH86162.1"
                     /translation="MSIFRRSDSTRSGKTPEPVKVAWANHQAEAELIAGILREEGIPS
                     LIKRNKGFDVPDFLAAGARDIFVPASAADRAREILDDLKSDEDRLPGS"
     gene            complement(1950..2348)
                     /locus_tag="PLE93_03600"
     CDS             complement(1950..2348)
                     /locus_tag="PLE93_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNH86163.1"
                     /translation="MKVPQSLLDEMVEHSKADAPDECCGLIGGAGDEAITLHRMENTA
                     HSPLRYEMDSKEQLKVYKQILEAGHDVVGIYHSHTRTAAYPSQTDINLASGWPDAVYF
                     IVSLENPDEPYVRGFNIREGQVEDVDIQAV"
     gene            complement(2359..>3102)
                     /locus_tag="PLE93_03605"
     CDS             complement(2359..>3102)
                     /locus_tag="PLE93_03605"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNH86164.1"
                     /translation="GQTILEPTSGNTGISLAMICRRKGYPLKVVMPDNVTAERSQLLE
                     MYGAEIVYSEGAKGSNGAVELALEMAEDPQYYMPYQYGNEANPNAHYDGTAVEILEEL
                     DEVTAFVAGLGTGGTLMGNGRRLKEANPETRIVAAEPMQGELVQGLRSLDDGFIPPII
                     DLSLLDRKIMVSNMDAIIWTKKLLSQEGIFAGVSSGAVAAIAVRIANEIDSGNIVFLI
                     PDGGWKYLSSGVYTKSIEELGDIDATNWW"
BASE COUNT          546 a         1007 c          998 g          551 t
ORIGIN      
        1 acctgtaccc ttggtggtcg cggctgaggg gctggaaaag cattccggac ctcgctatac
       61 tttttgaagt aaccaaattc agatcgagat tgagggtttt tgccgatggc accggaagcg
      121 accgaacagc agacaatcga agagatcacg ccccaggagg ctttcgccga gctcgatggt
      181 ggcggcgtca agctgatcga cacccgcgag ccctacgagt acgaggagtc tcacatcgag
      241 ggcggcgagt tgatcccgcc cggagtgatc gccgaccgga tcgaagcggc cgtgcccgac
      301 aaggaccagc ggatcatcct gcagtgccgc tccggtgccc gctccctggt cgcggcagaa
      361 gctctggcgg ggctcggcta cacgaacctc gccaacatga gcggcggcat cctcgaatgg
      421 gaggcccagg gcctgccggt cgagtcgaac gtcgccctga cccccgaaca gcgtgaccgc
      481 tactcgcggc acatcctgct gcccgaggtc ggggtcgaag gccagatcaa gctgctcgaa
      541 gcgaaggtgc tgctgctcgg cgccggcggt ctcggagcgc ccacagcgct ctatctcgcg
      601 gccgcaggca ttggcacgat cggcatcgtc gatgacgacg tggtcgacgc ctccaacctg
      661 cagcgccagg tgatccacaa caccagccgg gtcggccagc ccaagtccgc ctcggcccgc
      721 cagacgatcg aggaactgaa tcccgacgtc gaggtgatcg aatacaactt ccggctcgac
      781 gccggcaaca tcctcgaggt gatcgaggac tacgacatca tcgtcgacgg ggccgacaac
      841 ttcccgaccc gctaccttct gaacgacgcc tcggtgcggc tgaagaagcc ggtcgtctcc
      901 gcctcgatcc tttccttcga cgggcagatc tcgaccttca tcccttacga aggcccctgc
      961 taccgctgcc tctacccgac cccgccgccg ccggagctcg ccccgagctg ctcggccaac
     1021 ggcgtgctcg gtgcgatggc cgggcagatg ggcctgctcc aggcgaacga agtcctgaag
     1081 ctggtgctcg gcatcggcga gccgctggtt ggccgcctgc tgctctacga agcactcgga
     1141 acccgcttca ctgaactcaa ggtccggcgc gatccggaat gcccgatctg cggtccgaac
     1201 gcgcccaagg tcccggaatc cgagatgggc cagttccccg actacgaggc attctgcgga
     1261 ggtcatgtcg gctcctgagc cggcgtgacc gacccccgca gacgaccgat aggatcagcc
     1321 catgccgtcc gtgaagattc cccccgttct ccgtaagcac accggcgatc agacgtcagt
     1381 cgacgttgcc ggttcgaacg tgggcgaggt gctgaacgcg ctcgtcgccg agtaccccga
     1441 aacgaaggac cagctcttct cgggcgaagg tgagctcaac cgcttcgtca acgtctacct
     1501 gaacgacgaa gacgtccggg tgcttgacgg cctcgaaacc ggggtcagcg acaccgacac
     1561 cgtgatgatc ctcccggcca tggccggcgg cgaataactg caagtcgggc ggggtccgac
     1621 ccgccctccc agtaaaaccc gccccaccca ctcgcacaca agaatggatg ggcggggctc
     1681 cgatcgagaa tcttcaagaa ccgggcaggc ggtcttcgtc tgacttgagg tcgtcgagga
     1741 tctccctggc gcggtccgcg gcggaggcgg ggacgaagat gtcgcgggcg ccggcggcga
     1801 ggaagtccgg aacgtcgaat cccttgttgc gtttgatcag cgaggggatg ccctcctccc
     1861 ggaggattcc cgcgatcagt tcggcttcgg cctgatggtt ggcccaggcg accttgaccg
     1921 gctcgggcgt cttgcccgaa cgcgtggaat cagaccgcct gaatatcgac atcctcgacc
     1981 tgcccctcgc ggatgttgaa gccccggaca tacggctcgt ccgggttttc cagcgagacg
     2041 atgaagtaga cggcgtcggg ccatccgctg gcgaggttga tgtcagtctg cgaggggtac
     2101 gccgcggtcc gggtgtggga gtggtagatc ccgaccacgt cgtggccggc ttcgaggatc
     2161 tgcttgtaga ccttgagctg ctccttcgaa tccatctcgt agcggagcgg ggagtgggcg
     2221 gtgttctcca tccggtggag agtgatcgcc tcgtcgccgg cgccgccgat caggccgcag
     2281 cattcgtccg gagcatcagc cttgctgtgc tcgaccatct cgtcgagcag ggactgggga
     2341 actttcatcg gagcgggcct accaccagtt ggtcgcgtcg atgtcgccga gttcctcgat
     2401 cgacttcgtg tagacgccgg aggagaggta cttccagccg ccgtcgggga tcaggaagac
     2461 gatgttgccc gagtcgatct cgttcgcgat ccgtaccgcg atcgcggcga ccgcaccgga
     2521 agaaaccccg gcgaagattc cttcctggga aagcagcttc ttcgtccaga tgatcgcgtc
     2581 catgttcgag accatgatct tccggtcgag cagggaaagg tcgatgatcg gcgggatgaa
     2641 gccgtcatcg agggagcgga ggccctggac cagctcgccc tgcatcggct cggccgcgac
     2701 gatcctggtc tccgggttcg cttccttcaa gcgacggccg ttgcccatca gggtgccgcc
     2761 cgtgccgagc ccggccacga aggcggtcac ctcgtcgagc tcctcgagga tctcgaccgc
     2821 ggtgccgtcg taatgtgcgt tcggattggc ctcgttgccg tactggtaag gcatgtagta
     2881 ctgcgggtcc tcggccatct ccagggcaag ttcgaccgct ccgttggaac ccttggcgcc
     2941 ttccgagtag acgatttcgg cgccgtacat ctcgagcagc tggctgcgct cggcggtgac
     3001 gttgtcgggc atgaccacct tgagcgggta gcccttgcgg cggcagatca tcgccagcga
     3061 gatgccggtg ttgccggagg tcggttccag aatggtctgg cc
//
LOCUS       DAOQFI010000148         3087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15658_length_3087_cov_3.504288, whole genome shotgun sequence.
ACCESSION   DAOQFI010000148 DAOQFI010000000
VERSION     DAOQFI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15658_length_3087_cov_3.504288"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /gene="infB"
                     /locus_tag="PLE93_03610"
     CDS             <1..1730
                     /gene="infB"
                     /locus_tag="PLE93_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNH86165.1"
                     /translation="VRDLAELLGLSSADLIKKLMTMGEMATLTQTLTDDSVKAIADEY
                     DRKIEIVSAAQEEAPAPEFEDSDEELMDRPPVVTIMGHVDHGKTSLLDAIRETEVAAG
                     EAGGITQHIGAYQVHHDDHTITFLDTPGHEAFTAMRARGAQVTDVAVIVVAADDGVMP
                     QTREAIDHARAADVPILIAVNKIDKEGANPDRIRTDLAADGLSPEDWGGDTIYCNVSA
                     KTNEGLDNLLDMIILVTELEELVANPDAPASGSVIESKLDPGRGPVVSVLVNRGTLRV
                     GDSVVAGAVWGKVRAMHDYLGAKVDEATPGMPVEVLGFDGVCEAGEFVQVVENDRRAR
                     QMAQERLNRLKAETIARRSARKVTLEEIFARAQTGDLKELNIVLKSDVAGSLEALQDE
                     IAKVPQEQVAINIIRAQTGGITESDVMLASASNAVIIGFNVRPLADARRAAQAEGVDI
                     RTYSVIYKITEDLRAAMEGMLDTVEVEDSIGEAEVREVFKASKVGRIAGCVVTEGRVQ
                     RDASVRLVREGTVIWTGQLDSLRRFKDAVQTVEEGQDCGIVLDGYADVKVGDVLEFFS
                     TRQVEQTLS"
     gene            1756..2103
                     /gene="rbfA"
                     /locus_tag="PLE93_03615"
     CDS             1756..2103
                     /gene="rbfA"
                     /locus_tag="PLE93_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNH86166.1"
                     /translation="MRRVNEVLRQVISDAITNDLSDPRLEMATVTSVDTTPDLRHAKV
                     YFTVLGDDAAKADALKGLGSAHSLLQGKVAAETRMKHTPALAFEYDESIESGMRIDAL
                     LAEGEAKGREDGE"
     gene            2100..>3087
                     /locus_tag="PLE93_03620"
     CDS             2100..>3087
                     /locus_tag="PLE93_03620"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNH86167.1"
                     /translation="MNPAAASQLVVKGDLEEIATLIREEQKFLLTTHEGPDGDALGSL
                     LGMHHLLESLGKDSVMFLAAKEFPLPIEYRHLPLEGVFHEPPADIRDRIVVFLDCGNI
                     DRMPVDFLREDGKLMVNIDHHHDNTEFGDLNLVDAGASCTAEIIYELSVLLDVKVSPA
                     MARALYIGIVTDTGKFMYDATRPKTHRVAAELIEAGVDVDDVNRRLYESMPIEKLRLL
                     SRALDNLELHCEGQLIVSYITDEDYRKSGAGEEMTEGIIDNLRAVDGIRVAAVVRDQV
                     SRGRAARKISLRAGDDHTDVSVLARKFGGGGHVRAAGCSTELSFDEIVSELCS"
BASE COUNT          607 a         1011 c          996 g          473 t
ORIGIN      
        1 cggtccgtga cctggccgaa ctgctcggcc tcagttccgc cgacctgatc aagaagctga
       61 tgaccatggg cgagatggca accctcaccc agaccctgac cgacgattcg gtcaaggcga
      121 tcgccgacga gtacgaccgc aagatcgaga tcgtctcggc ggcgcaggag gaagccccgg
      181 ctccggaatt cgaggattcc gatgaggaac tcatggaccg cccgcccgtg gtcacgatca
      241 tgggtcacgt cgaccacggc aagacctcgc tgctcgacgc aattcgcgag accgaagtcg
      301 ccgccggcga ggccggcggg atcacccagc acatcggtgc ttaccaggtc catcacgacg
      361 accacacgat caccttcctc gacaccccgg gccacgaggc cttcaccgcc atgcgggccc
      421 gtggcgccca ggtcaccgat gttgcggtga tcgtggtcgc cgccgatgac ggcgtcatgc
      481 cccagaccag ggaagcgatc gatcacgccc gggccgccga cgttccgatc ctgattgcgg
      541 tcaacaagat cgacaaggaa ggtgcgaatc ccgaccggat ccgcaccgat cttgccgccg
      601 acgggctcag cccggaggat tggggcgggg acacgatcta ctgcaacgtg tcggcgaaga
      661 ccaacgaggg actcgacaac ctgctcgaca tgatcatcct ggtcaccgag ctcgaggaac
      721 tcgtcgcgaa ccccgatgcg cccgcatccg gttccgtgat cgagtccaag ctcgatcccg
      781 gccggggccc ggttgtttcc gtgctggtca accggggcac gctccgggtc ggcgactcgg
      841 tcgtggccgg agccgtctgg ggcaaggtcc gggcgatgca cgactacctc ggcgccaagg
      901 ttgacgaagc aacccccggc atgccggtgg aggtgctcgg tttcgatgga gtctgcgaag
      961 ccggggagtt cgtgcaggtg gtcgagaacg accgtcgcgc caggcagatg gcccaggaaa
     1021 ggctcaaccg cctcaaggcc gaaaccatcg cccgccgttc cgcccgcaag gtcaccctcg
     1081 aggagatctt cgcccgcgcc cagaccggcg acctcaagga actcaacatc gtgctcaaga
     1141 gcgatgtcgc gggttcgctg gaggcgctgc aggacgaaat cgccaaggtg ccgcaggagc
     1201 aggtcgcgat caacatcatc cgcgcccaga ccggcggcat caccgagtcc gacgtgatgc
     1261 tcgcttcggc ctcgaacgca gtgatcatcg gcttcaacgt gaggcccctt gccgacgccc
     1321 gccgggccgc gcaggccgag ggggtcgaca ttcgcaccta ctccgtcatc tacaagatca
     1381 ccgaagacct gcgggccgcc atggaaggca tgctcgacac ggtcgaggta gaggattcga
     1441 tcggtgaagc ggaagtccgg gaggtcttca aggcctccaa ggtcggccgg atcgccggat
     1501 gcgtcgtcac cgaagggagg gtccaacggg acgcctcggt gcggctcgtc agggagggga
     1561 ccgtcatctg gacggggcag ctcgattcgc tgcgccgttt caaggatgcg gtccagaccg
     1621 tcgaggaagg ccaggactgc ggcatcgtcc tggacgggta cgccgacgtg aaggtcggcg
     1681 acgtactcga gttcttctcg acccgtcagg tcgaacagac cctcagttag gcggcaagcg
     1741 gtgtcgaagg cgcggatgcg cagggtgaat gaggtgcttc gtcaggtgat ttccgatgcg
     1801 atcaccaacg atctgagcga tcctcgtctg gaaatggcaa ccgtcacctc ggtcgacacc
     1861 accccggacc tgcgtcacgc caaggtctat ttcacggtgc tgggtgacga tgcggcaaag
     1921 gccgacgcgc tcaagggcct gggcagcgcc cacagcctgt tgcagggcaa ggtggcggcc
     1981 gagacccgga tgaagcacac cccggcactg gccttcgaat acgacgaatc gatcgagtcg
     2041 ggaatgcgaa tcgacgccct gctggccgaa ggcgaagcca aaggcaggga ggacggcgag
     2101 tgaatcccgc tgccgccagc cagctcgtgg tgaaggggga tctcgaggag atcgcgaccc
     2161 tgatcaggga ggaacagaag ttcctcttga cgacccacga gggaccggac ggagatgccc
     2221 tcggctcgct gctggggatg catcacctgc tggaaagcct cggcaaggac tccgtgatgt
     2281 tcctcgccgc gaaggagttc ccgcttccga tcgagtaccg ccacctgccg ctcgagggcg
     2341 tcttccacga accgccggcc gacatccgcg accggatcgt cgtctttctc gattgcggca
     2401 acatcgaccg catgccggtc gacttcctgc gcgaagacgg caagctgatg gtcaacatcg
     2461 accatcatca cgacaacacc gaattcggtg acctgaacct ggtcgacgcc ggtgcctcct
     2521 gcaccgccga gatcatctac gaactttcgg tcctgctcga cgtcaaggtc agccccgcga
     2581 tggcccgggc gctttacatc gggatcgtca ccgacaccgg caagttcatg tacgacgcga
     2641 ccaggcccaa gacccaccgg gtcgccgccg agctgatcga agccggagtc gacgtcgatg
     2701 acgtgaaccg tcgcctctac gaaagcatgc cgatcgagaa actgcgcctg ctgagccggg
     2761 ccctcgacaa cctcgaactt cactgcgaag gacagctcat cgtcagctac atcaccgacg
     2821 aggattaccg caagagcggg gccggggagg agatgaccga agggatcatc gacaacctgc
     2881 gggcggtcga cggaatcaga gtcgctgccg tggtgcgcga ccaggtcagc cgcggccgcg
     2941 cggcccgcaa gatcagcctc cgcgccggtg acgaccacac cgacgtctcg gtgctggccc
     3001 ggaagttcgg cggcggtggc cacgtgcggg ccgccggctg ctcgaccgag ctctccttcg
     3061 atgagatcgt ctccgaactc tgcagcg
//
LOCUS       DAOQFI010000149         3076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15751_length_3076_cov_2.980470, whole genome shotgun sequence.
ACCESSION   DAOQFI010000149 DAOQFI010000000
VERSION     DAOQFI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15751_length_3076_cov_2.980470"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLE93_03625"
     CDS             complement(<1..949)
                     /locus_tag="PLE93_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNH86168.1"
                     /translation="MTKVDLDPALRAALGGGPERHHAKTAEQGKLPVRERVDLLLDEN
                     SFVEDALLANWDQEGFGADGVVTGTGLIWGRPVALMANDPTVKAGSWGPKTVEKILRI
                     QERALKLEIPMIYLVDSAGARITDQVQMFPGRRGAGRIFYNEVKMSGRVPQVCILFGP
                     SAAGGAYIPAFCDVVIMRDGNASMYLGSPRMAEMVIGETVSLDEMGGARMHTSKSGCG
                     HFLVKTDEEAIETGRSYMRYMPTSWREQVPDVEPKKPVSDTPLEEIIPLDENVPFDIN
                     LLLDTLLDEGSFIEVHKRWAKELIVGYSRLDGKAVGIVAN"
     gene            complement(958..1842)
                     /locus_tag="PLE93_03630"
     CDS             complement(958..1842)
                     /locus_tag="PLE93_03630"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNH86169.1"
                     /translation="MEIKKVGVVGCGLMGHGITQVSAQAGYDVIVADVNQEALDKGLG
                     KISKLLGKSVEKGKIEQADADAILGRINTTTDLNDFADCDLVIEAVTENLETKLKIWK
                     QLDGIVKDEAYFATNTSSLPVINQAAATTRPTNFLGLHFFNPVQVMPLLEVIQSVTTS
                     PEAMGIGLAYGEKLKKTTVETKDKAGFIVNRLLVPYMLDAIRGLEEGIGTIDEIDAAM
                     KAGAGHPMGPLTLADFVGLDTLHSISDVMFDEFRERRFAAPPTLRKMVAAGFYGKKSG
                     RGFYDYSGEKPVSVNPGL"
     gene            complement(1921..2268)
                     /locus_tag="PLE93_03635"
     CDS             complement(1921..2268)
                     /locus_tag="PLE93_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86170.1"
                     /translation="MNRIRTFLIALVAIALVAGPIGLPSQAGASPDASASASAKAKKK
                     CKKFKGKKKRQCLKNASKGGGKPGNGGKPWAGSPYKPGITCAMSPEMQKKYARYGLTC
                     LDLGLGVTTLIEL"
     gene            complement(2265..2645)
                     /locus_tag="PLE93_03640"
     CDS             complement(2265..2645)
                     /locus_tag="PLE93_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH86171.1"
                     /translation="MKSDGFRAAAEAKDFEQGRDLFTEDVVFRSPFVHTPYEGIDALG
                     FLLGHVVQVFEDFHYIAHVETDDVAVLQFEARVGDKELQGVDILKFNDEGKICEMTVM
                     VRPASGLEAVGMAMKSRIEAATAS"
     gene            complement(2698..>3076)
                     /locus_tag="PLE93_03645"
     CDS             complement(2698..>3076)
                     /locus_tag="PLE93_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86172.1"
                     /translation="LFSTGGSLVFSEGYPLGPAGYLIDGYLVIERDDVGDVNASCEIF
                     RVFVNSSDIQTGTAETVGLPRTTVAGNGFATLPISGVLDLSSPPGDADGVEMRVVCET
                     NTDNAIAAERQVNAVPTDQLIPG"
BASE COUNT          542 a          961 c          958 g          615 t
ORIGIN      
        1 ggttggcgac tatcccgacc gccttgccgt cgagccggct gtagccgacg atcagctcct
       61 tggcccagcg cttgtgaacc tcgatgaagc tgccctcgtc gaggagcgtg tcgagcagga
      121 ggttgatgtc aaaggggacg ttctcgtcaa gcggaatgat ctcttcgagc ggggtgtcgg
      181 agaccggctt cttcggctcg acgtcaggga cctgctcgcg ccagctggtc ggcatgtacc
      241 gcatgtagct gcgaccggtc tcgatcgcct cttcatcggt cttgaccagg aagtggccgc
      301 aacccgactt ggaggtgtgc atgcgggccc cgcccatctc gtcgagtgaa accgtctcgc
      361 cgatgaccat ctcggccatg cggggagaac cgaggtacat cgaggcgttt ccgtcccgca
      421 tgatcacgac atcgcagaag gcgggaatgt aggcaccgcc ggccgcgctg ggaccgaaca
      481 ggatgcagac ctgcggtacc cggccgctca tcttcacttc gttgtagaag atccggcccg
      541 ctccgcggcg gccggggaac atctggacct gatcggtgat ccgggccccg gccgagtcga
      601 ccaggtagat catcgggatc tccagcttca gggcccgttc ctggatccgg aggatcttct
      661 cgaccgtctt cgggccccag gaaccggcct tgacggtcgg atcgttggcc atcagggcaa
      721 ccggccggcc ccagatcagc ccggttccgg tgacgacgcc atcggcgccg aagccctcct
      781 ggtcccagtt ggccaggagc gcgtcctcga cgaaagagtt ttcgtcaagc agcagatcga
      841 cccgttcccg gaccgggagc ttgccctgct ccgcggtctt ggcgtgatgc ctttccggac
      901 cgccgccgag ggcggcccgg agggcgggat cgagatcgac cttggtcaac ggaagcccta
      961 caggcccgga ttgaccgaga ccggcttttc gccggagtaa tcgtagaaac cgcggcccga
     1021 cttcttgccg tagaaaccgg cggcgaccat cttgcgaagg gtcggcgggg cggcgaaacg
     1081 acgctcacgg aactcgtcga acatcacgtc ggaaatcgag tgaagggtgt cgagcccgac
     1141 gaagtcggcc agggtgagcg ggcccatcgg gtgaccggca ccggccttca tcgccgcatc
     1201 gatctcgtcg atcgtgccga tgccctcttc gaggccgcgg atcgcgtcga gcatgtacgg
     1261 aaccagcagg cggttgacga tgaagccggc cttgtccttg gtttcgacgg tggtcttctt
     1321 cagcttttcg ccgtatgcga gaccgatgcc catcgcctcg ggcgaggtgg tcaccgactg
     1381 gatcacctcg agcaggggca tgacctggac cgggttgaag aagtgcaggc cgaggaaatt
     1441 ggtcggccgt gtggtggccg cggcctggtt gatgaccggc agcgaggaag tgttggtcgc
     1501 gaagtaggcc tcgtccttga cgatgccgtc gagctgcttc cagatcttga gcttggtctc
     1561 gagattctcg gtcacggcct cgatcacaag gtcgcaatcg gcgaagtcgt tcagatcggt
     1621 ggtggtgttg atccggccga ggatcgcgtc ggcgtcagcc tgttcgatct tgcccttctc
     1681 gaccgacttg ccgagcagct tgctgatctt gccgaggccc ttgtcgagcg cttcctggtt
     1741 gacatcggca acgatgacgt cgtatccggc ctgggcgctg acctgggtga tgccgtggcc
     1801 catcagaccg cagccaacaa ccccaacctt cttgatctcc atctggagcc ttcctcctgt
     1861 agcgaagttc gtgaatgccc ccgggacggc ggaggcgacc ggccacggag cctattcgat
     1921 ctaaagctcg atcagggtgg tgacgccgag gcccagatcg agacaggtca ggccgtacct
     1981 ggcgtacttc ttctgcatct cgggactcat cgcgcaggtg attccgggct tgtagggact
     2041 gccggcccac ggctttccgc cattgcccgg ctttccgcca cccttcgaag cgttcttcag
     2101 gcactgtcgc ttcttcttgc ccttgaactt cttgcacttc ttcttcgcct tggccgaagc
     2161 cgaagctgag gcgtccggtg aagcaccagc ctgtgatggc aggccgatcg gaccggcgac
     2221 cagggcgatc gccaccaggg ctatcaggaa ggtccggatg cggttcacga ggccgtcgcc
     2281 gcttcgatcc ggctcttcat agccatgccg acggcctcga gaccggaagc aggcctgacc
     2341 atgacggtca tttcgcagat cttgccttcg tcgttgaact tgaggatgtc gacgccctga
     2401 agttccttgt ctccgacccg ggcctcgaac tggagcacgg caacatcgtc ggtctcgacg
     2461 tgggcgatgt agtgaaaatc ctcgaagacc tggaccacat ggccaagcag gaaaccgagc
     2521 gcgtcgatgc cctcatacgg ggtatgaacg aaggggctgc ggaagacaac atcctccgtg
     2581 aacagatccc tgccctgctc gaaatccttt gcttccgccg ctgccctgaa accgtcgctc
     2641 ttcatatgcc gaaacatata gctgactgaa acacgcgtgc tttctgtgcc gcgttagcta
     2701 ccccgggatc agctggtcgg tgggtaccgc gttcacttgg cgctctgccg caatagcgtt
     2761 atctgtgttt gtctcgcaga cgactctcat ttcgacaccg tcggcgtcac cgggggggct
     2821 tgaaaggtca agtactccgc tgatgggcag ggttgcgaat ccgttaccgg caaccgttgt
     2881 tctgggcagc cccaccgtct ccgccgtccc ggtctggatg tcggacgaat tcacgaagac
     2941 ccggaaaatc tcgcagctgg cgttcacgtc tccaacatcg tcgcgttcga tcaccaggta
     3001 tccgtcaatc aggtaaccag ctggccccag tggatagccc tcactgaaga ccagagagcc
     3061 tcctgtcgag aacagg
//
LOCUS       DAOQFI010000150         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15796_length_3070_cov_4.188723, whole genome shotgun sequence.
ACCESSION   DAOQFI010000150 DAOQFI010000000
VERSION     DAOQFI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15796_length_3070_cov_4.188723"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLE93_03650"
     CDS             complement(<1..386)
                     /locus_tag="PLE93_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNH86173.1"
                     /translation="MKTEIRHNPGFAIARCTLAPGEQMRAESGAMAATSAGVEIEAKV
                     QGGLMKGLKRSVLGGESLFMTTLTAPQSGGWVDVAANLPGDAMVMSTGEELFLTRGTY
                     LASDASVDIDTKWGGFKNIGGGEGGFL"
     gene            complement(454..1803)
                     /locus_tag="PLE93_03655"
     CDS             complement(454..1803)
                     /locus_tag="PLE93_03655"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNH86174.1"
                     /translation="MHDIECPHCGKAFTIDEAGYADILNQVRDEAFEKAIHDRLELAE
                     REKLAAVELARTKAASEHKDAATEKDLEIERLREELKASSELARAKATGELKDESAKK
                     DAEIERLKAELDKADVAGKLALKEALGEVEKERDDLKRSLESKDTEQKLLETSLKEKY
                     EIQIKDRDGEIERLKDMKAKLSTKMIGETLEQHCEIEFNKIRAAAFPNAVFGKDNDAS
                     AGTKGDFIFREADPEGTEFASIMFEMKNELDDGENKKKNEDFFRKLDDDRKKKGCEYA
                     VLVSLLEPENELYNAGIVDVSHLYPRMYVIRPQFFIPIITLIRNEALKTLKLRSELAQ
                     VRAQNVDITNFEEEMENFKSGFQRNFGIASDQFDKAIEQIDKSIANLGKTKDWLLKSA
                     NQLRLANDKAQDLSIKKLTRGNETMKAKFEEARSPDLEEIKRLEIAESDSGPDSRAA"
     gene            1977..2645
                     /locus_tag="PLE93_03660"
     CDS             1977..2645
                     /locus_tag="PLE93_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HNH86175.1"
                     /translation="MRELRPGTVIAVIALFIAIGGTATAASGLINGSKIKPGTVTARQ
                     IKNQTITTSKLAPATVASLRGARGPVGATGPAGENGQPGTPGQPGQPGAVGATGATGA
                     TGADGVVEPYMDQVLNFNLPTEDFTIPLSIDLPPGQYLLTAKTNVVNQYASGDNMIEC
                     TIWTDEVDGVDRATSDVPYNRIFNLSAMAVADVQGLVELRCVAWNGPAQLFDTKLIAQ
                     PIQG"
     gene            complement(2647..3063)
                     /gene="arfB"
                     /locus_tag="PLE93_03665"
     CDS             complement(2647..3063)
                     /gene="arfB"
                     /locus_tag="PLE93_03665"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNH86176.1"
                     /translation="MKPGIRPRGGPFIPVGEITLRASRSSGPGGQHANVTASRIEAVF
                     EVESSLALNDAEKARIIRKLGPTVTAVSQDARSQTRNRDLALERLETKLAGALSVPRR
                     RRATRPTRASKQKRLESKRHQSRKKQDRRRPGPDYD"
BASE COUNT          486 a          971 c          974 g          639 t
ORIGIN      
        1 aggaagccgc cctcgccgcc gccgatgttc ttgaagccgc cccacttggt gtcgatgtca
       61 acgctggcgt ccgaggccag ataagtaccg cgggtgagga agagttcctc cccggttgac
      121 atgaccatcg cgtcgccggg caggttcgcc gcgacgtcga cccagccccc ggactgcggc
      181 gcggtcaggg tggtcatgaa aagcgactcg cccccgagca ccgaacgttt cagccccttc
      241 atcagtccgc cctgcacctt ggcttcgatc tcgactccgg ccgaggtggc ggccatcgcc
      301 cccgactcgg ctctcatctg ctcgccgggg gcgagcgtgc atcgagcaat tgcaaaaccg
      361 gggttgtgac gaatctcagt tttcatgcgc ccaccctagc cgtcaccgcg aaggcaaacg
      421 ccggacaaca gttccggggg agttccggag aactcaggca gcccgggagt cgggcccgga
      481 atcggactcg gcgatttcca gccgcttgat ctcttcgaga tccggcgagc gggcttcctc
      541 gaacttggcc ttcatcgttt cgttgccccg cgtgagtttc ttgatcgaca ggtcctgggc
      601 cttgtcgttg gcaagacgca actggttggc agacttgagc agccaatcct tggtcttgcc
      661 gagatttgca atggacttgt cgatttgttc gatcgccttg tcgaactgat ccgaggcgat
      721 cccgaagttt cgctggaagc cgctcttgaa gttctccatc tcctcctcga agttggtgat
      781 gtcgacgttc tgggcccgca cctgggccag ctctgatctg agcttcaggg tcttgagggc
      841 ttcattccgg atcagcgtga tgatcgggat gaagaactgt ggccggatca catacatacg
      901 gggatagaga tgcgaaacgt cgacgatgcc ggcgttgtag agctcgtttt ccggttcaag
      961 cagcgagacc aggacggcgt actcgcagcc cttcttcttc cggtcgtcat ccagcttcct
     1021 gaagaagtcc tcgttcttct tcttgttttc gccgtcgtcg agctcgttct tcatctcgaa
     1081 catgatcgag gcgaactcgg ttccttccgg gtcggcctcg cggaagatga agtcgccctt
     1141 ggtgccggcg ctggcgtcgt tgtccttgcc gaacacggca ttcgggaaag cggcggcgcg
     1201 gatcttgttg aactcgatct cgcagtgctg ctccagggtc tcgccgatca tcttggtcga
     1261 gagcttcgcc ttcatgtcct tcaggcgttc gatctcgccg tcgcggtcct tgatctggat
     1321 ctcgtacttc tccttgagtg aagtctcgag aagcttctgc tcggtgtctt tggactcaag
     1381 cgaccgcttg aggtcgtcgc gttccttctc gacttcgccc agggcttcct tgagggcgag
     1441 cttcccggcg acgtccgcct tgtccagctc tgccttcagg cgttcgatct cggcgtcctt
     1501 cttcgcgctt tcgtctttga gctctccggt cgccttggcc ctggccagtt cggaactcgc
     1561 cttcagctct tcccgcaggc gctcgatctc caggtccttc tcggtggcag cgtccttgtg
     1621 ctcgctggcc gccttggtcc gggccagctc gaccgccgcc agcttctccc gttcggcgag
     1681 ttccagccgg tcatggatcg ccttttcgaa ggcttcgtca cgaacctggt tgaggatgtc
     1741 cgcgtaaccg gcctcgtcga tggtgaaggc tttcccgcaa tgcgggcatt cgatgtcatg
     1801 catttccaag gctcctttcg gtttcgccga cggcccttca gcttaggcgg tggtcaggat
     1861 gctcagactg agtcgccttc ggattgcggg ttctgacatg ctccacgctt gaagaccgcc
     1921 ggcacggccg cattcggctc cagggcaggc agggaaagaa ccagcaagga gcaagaatga
     1981 gggaattacg accgggcacg gtcattgccg tgatcgcgct gttcatcgcg atcgggggca
     2041 ccgcgacggc agcgtcggga ctgatcaacg gaagcaagat caagccggga accgtcaccg
     2101 ccaggcagat caagaaccag acgatcacca cctccaagct cgcgccggcg acggtcgctt
     2161 cgctgcgagg cgcccgtggc ccggtcggag caaccgggcc ggcgggggaa aacggtcagc
     2221 caggcacccc gggccagccg ggtcagccgg gcgcggtcgg agcgaccgga gccaccggag
     2281 ccacgggcgc cgacggcgtg gtcgagccgt acatggatca ggtcctgaac ttcaacctgc
     2341 cgaccgagga tttcacgatc ccgctctcga tcgatctgcc tcccggccag tacctgctca
     2401 ccgccaagac caacgttgtg aaccagtacg catccggcga caacatgatc gagtgcacca
     2461 tctggaccga tgaggtcgac ggtgtcgacc gggcgacgag cgacgtgccg tacaacagga
     2521 tcttcaacct ctcggcgatg gccgtggcag atgtgcaggg tctggtcgaa ctgcgctgcg
     2581 tcgcctggaa cggtccggcc cagctgttcg acaccaagct gatcgcccag ccgatccagg
     2641 gctgattcag tcgtaatcgg gtccggggcg tcgccggtcc tgcttcttgc gggactggtg
     2701 gcgcttcgac tcgaggcgct tctgcttcga ggcgcgggtg ggcctggtcg cacggcgccg
     2761 gcgcgggacc gaaagcgcgc cggccagctt cgtttcgagt ctctcgaggg cgaggtcgcg
     2821 gttgcgggtc tgggagcggg cgtcctggct gaccgcggtg accgtcggtc cgagcttccg
     2881 gatgatcctc gccttctcgg catcgttcag ggcgagtgag gactcgacct cgaagacggc
     2941 ctcgatccgc gaggcggtga cattggcgtg ctggccaccc gggccggagg accgggaagc
     3001 tcgcagggtg atttcgccga ccgggatgaa gggcccgccg cggggtcgga tgccgggctt
     3061 catcgggccg
//
LOCUS       DAOQFI010000151         3068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15821_length_3068_cov_2.797544, whole genome shotgun sequence.
ACCESSION   DAOQFI010000151 DAOQFI010000000
VERSION     DAOQFI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15821_length_3068_cov_2.797544"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /gene="glsA"
                     /locus_tag="PLE93_03670"
     CDS             complement(<1..788)
                     /gene="glsA"
                     /locus_tag="PLE93_03670"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HNH86177.1"
                     /translation="MTESVGNNRTASPVQALLERLHARHAGDLSGEVASYIPELAGAD
                     PEWFGISMVTADGSVYEVGDTRREFTIQSISKPLTFALALEAIGEGEVRRHVDVEPSG
                     EAFNSITLQPGTGLPLNPMVNAGAIATASLIEPLGFEKPIDRILHEYSQFAGRHLKVD
                     EQVFTSENETGNRNRAIAYLLKNAGVIDRDVEEVVETYFRQCSTLVTCRDLATMAATL
                     ASTGVNPVTGERVVREDTVRNVLSVMASCGMYDAAGDWLYTVGLP"
     gene            861..2249
                     /locus_tag="PLE93_03675"
     CDS             861..2249
                     /locus_tag="PLE93_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNH86178.1"
                     /translation="MLPLADTTVDLARLQFATTSIYHFLFVPLTLGLTPLVAVMQTLW
                     HRTGDERWYRLTRFFGTLMLINFAIGVATGIVQEFQFGMNWSTYSSYVGDVFGAPLAI
                     EGLAAFFLESVFLGLWIFGWDLLPKRVHLAMIWLAAIGTWLSAYFILVANSWMQRPVG
                     YELNGDKAQLNDVGALLSNRFAIHAFEHTLLVGLSTAALFIFGIACWHLVKGRNVETF
                     RKAAALALIVGVPATGINLAVGSGFGIVTTDYQPMKIAGSEALWNTEQPAGFSLFQIG
                     GFSQDDQDPSVVIQIPKLLSFLATGSVNGKVEGMNQLQADYQQKYGPGDYLPPVRVNY
                     WSMRVMAYTGTLMFLLMALGALFYWRGTLERRRRFLWAAVGGIALPYVAATAGWVLTE
                     MGRQPWIVQGLLKTADANSPAVSSGQIIASLSVFVLLYTCLAVADVTLMRRYARVDPP
                     GGDDSKLEAEPA"
     gene            2246..>3068
                     /gene="cydB"
                     /locus_tag="PLE93_03680"
     CDS             2246..>3068
                     /gene="cydB"
                     /locus_tag="PLE93_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNH86179.1"
                     /translation="MSLETVWFILIAVLWSGYFVLEGFDFGVGMLLPVLGRSESDRSA
                     MLNTIGPIWDGNEVWLIVAGGATFAAFPLWYATMFSGFYLALLAILVCLILRVVSFEW
                     RGKGDTPRWLGFWTWVNAVTSCLAAVIWGVALSNLLQGLPIDSSQEFTGNFLDLFSPF
                     TVFAGLAFCLLFLFHGAGFLSLRTVGDLGQRSARAARRLAAPALLAGILFMVWCVATA
                     VAENDRGVLGPLVPAVAGSLALCGAALAARARRPGLSFTLGAAGIVGVIATLFTGL"
BASE COUNT          455 a         1037 c          972 g          604 t
ORIGIN      
        1 ggcaggccga ccgtgtagag ccagtcgccg gccgcgtcgt acatgccgca ggaggccatc
       61 acgctgagca cgttgcggac cgtgtcctcc cggaccaccc gctcgccggt caccgggttg
      121 accccggtcg aagcgagtgt ggccgccatg gtcgccaggt cccggcaggt gaccagggtg
      181 gagcattggc ggaaataggt ttcgaccacc tcttccacgt cccggtcgat caccccggcg
      241 ttcttcagca gataggcgat cgcccggttg cggtttccgg tctcgttctc ggaggtgaag
      301 acctgctcgt cgaccttcag gtggcggccg gcgaactgcg agtactcatg gaggatccgg
      361 tcgatcggct tctcgaatcc gagcggctcg atcagcgagg ccgtggcgat tgccccggcg
      421 ttgaccatcg ggttcagcgg cagcccggtc ccgggctgca gggtgatcga gttgaaagcc
      481 tccccgctcg gctccacgtc gacatgccgg cgaacttccc cttccccgat cgcctccagc
      541 gccagggcaa aagtcagcgg cttggagatc gactggatcg tgaactcccg ccgcgtgtcc
      601 cccacctcgt aaaccgaccc gtcggcagta accatcgaaa tcccgaacca ctccggatcc
      661 gcccccgcca gctcggggat gtagctcgcc acctcccccg aaagatcccc cgcatgccgc
      721 gcatgcaacc gctccagcaa cgcctgaaca ggcgaggccg tgcggttatt gccgacggat
      781 tcagtcatgg ataggtactt gccatcgtat tgaccggctc aaacaacggt tctgtccggt
      841 ttgtgccgaa gtatgcaggt gtgctgccgc tggctgacac gaccgttgac ctggcgcggc
      901 ttcagttcgc gaccacctcg atctaccatt tcctcttcgt tccgctgacc ctcgggctga
      961 ccccgctggt ggccgtgatg cagaccctct ggcaccggac cggggacgaa cgctggtacc
     1021 ggctgacccg cttcttcggg accctgatgc tgatcaactt cgcgatcggg gtggcgaccg
     1081 gaatcgtcca ggagttccag ttcgggatga actggtcgac ctactcgagc tatgtcggtg
     1141 acgtgttcgg ggcgccgctg gcgatcgagg gcctggccgc cttcttcctc gagtccgtct
     1201 tccttggctt gtggatcttc ggctgggacc tgctgccaaa acgggtccac ctggcgatga
     1261 tctggctggc cgcaatcgga acctggctct ccgcctactt catcctggtc gccaattcat
     1321 ggatgcagcg gccggtcgga tacgaactga acggcgacaa ggcccagctc aatgacgtcg
     1381 gtgccctgct gagcaaccgc ttcgcgattc acgccttcga acacaccttg ctggtcgggc
     1441 tctcgaccgc ggccctgttc atcttcggca tcgcctgctg gcatttggtc aagggcagga
     1501 atgtcgagac cttccgcaag gcggctgcac tggccctgat cgtcggggtg ccggcgaccg
     1561 gaatcaatct cgcggttggc agcggcttcg gaatcgtcac caccgattac cagccgatga
     1621 agatcgccgg ctccgaggcg ctttggaaca ccgaacaacc ggccggcttc tcgctctttc
     1681 agatcggcgg cttcagccag gacgaccagg atccgtcggt ggtgatccag attccgaaac
     1741 tgctctcgtt cctggccacg ggatcggtga acggcaaggt cgaaggcatg aatcagctcc
     1801 aggccgatta ccagcagaag tacggccccg gcgactacct gccaccggtc cgggtcaact
     1861 actggtcgat gcgggtgatg gcctacaccg gcaccctgat gttcctgctg atggcgctgg
     1921 gggcgctgtt ctactggcgg ggaaccctgg aaaggaggcg gcgtttcctc tgggccgcgg
     1981 tcggcgggat cgcgctgccc tatgtcgcgg ccacggccgg ctgggtgctg accgaaatgg
     2041 gccggcagcc ctggatcgtt cagggcctgc tgaagaccgc ggacgcgaac tccccggccg
     2101 tgtccagcgg ccagatcatc gccagtctct ccgtcttcgt gctgctctac acctgcctgg
     2161 ctgtcgccga cgtgaccctg atgcgccgtt acgcgagggt cgacccgccg ggcggcgacg
     2221 attcgaaact ggaggccgaa ccggcatgag cctggaaacg gtctggttca tcctgatcgc
     2281 cgtgctctgg agcggctact tcgtgctgga agggttcgac ttcggggtgg ggatgctgct
     2341 gcctgtactg ggccggagcg agtccgaccg gagcgcgatg ctgaacacga tcgggccgat
     2401 ctgggacggc aacgaggtct ggctgatcgt cgccggcggc gcgaccttcg ccgcctttcc
     2461 gctctggtac gcgaccatgt tctccggctt ctacctggcc ctgctggcga ttctggtctg
     2521 cctgatcctc agggtcgtct ccttcgagtg gcgcggcaag ggcgacacgc cccgctggct
     2581 cggcttctgg acctgggtga acgcggtcac cagttgcctg gccgcagtga tctggggcgt
     2641 cgctctttcg aacctgctgc agggtctgcc aatcgattcc tcgcaggagt tcaccggcaa
     2701 cttccttgac ctcttctcac ccttcacggt cttcgccggg ctcgcgttct gcctgctctt
     2761 tctgttccac ggggccgggt tcctctccct gaggaccgtc ggcgatctcg gccagcggtc
     2821 ggccagagcg gcacgccggc tggcggcgcc ggccctgctc gccggaatcc tcttcatggt
     2881 ttggtgcgtg gcaaccgcgg tggcggaaaa cgaccgcggc gtcctcggtc cgctcgtccc
     2941 ggcggtggcc ggatcactcg ctctctgcgg cgcggccctc gccgcccgcg cccgacgccc
     3001 cggcctctcc ttcactctgg gagcggccgg catcgtcggc gtgatcgcca ccctcttcac
     3061 cgggctct
//
LOCUS       DAOQFI010000152         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_15856_length_3064_cov_3.406780, whole genome shotgun sequence.
ACCESSION   DAOQFI010000152 DAOQFI010000000
VERSION     DAOQFI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15856_length_3064_cov_3.406780"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLE93_03685"
     CDS             <1..480
                     /locus_tag="PLE93_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH86180.1"
                     /translation="RKVGSVGRPLLTSHLRIQGGEILVQGPTVSAGALDADGWLHTGD
                     VGWIDEDGFLYVQGRTDEMIITGGENVIPAEVEDVLVQHPEILEAAVIGRPDPEWQQA
                     VTAVVVARDGVVVDLDGVRSHCEGKLAPYKIPKAIELVQELPKTPSGKLHRAALLKS"
     gene            539..1543
                     /gene="ilvC"
                     /locus_tag="PLE93_03690"
     CDS             539..1543
                     /gene="ilvC"
                     /locus_tag="PLE93_03690"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNH86181.1"
                     /translation="MSTQDIFYDKDGDLSKLEGKTVAILGFGSQGHAHALNLKDSGVN
                     VVVGLREGSASREQAAAAGLEVLSVADASSKGDIVMILLPDEKQAEIWENEIKDGIAE
                     GNLLMFAHGFAIRFDQIVPPAGVDVGMVAPKGPGHMVRRQFEEGSGVPCLMAVEQDAT
                     GNAHDLVLAYAIGVGGGRAGIIETTFKDECETDLFGEQAVLCGGATELVRAGFDTLVE
                     AGYDPRLAYFECLHELKLIVDLMYEKGIQGMRYSISNTAEYGDLTRGKRVIGEDSRAA
                     MKEILGEIQSGEFAKEFIGENQAGNANFDRMRQEGKDHKVEIVGKELRDMMPWINQEF
                     "
     gene            1910..2563
                     /locus_tag="PLE93_03695"
     CDS             1910..2563
                     /locus_tag="PLE93_03695"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNH86182.1"
                     /translation="MTDTPTPTPGGEEEIVVSEVSESVVVPGGYPVDVDAQLLPEYSR
                     FMPLIKWLILLPHYIVLFFLGIGAMFVAFIAFFATLFTAKYPEGMWNYMVGVHRWALR
                     VMAYNFLITDNYPPFTLQETDEDTIQLVAEYPERVSRWRPFFAGLICIPYAIVASLIY
                     MVASICSFFAFFTILFTKKIPAGLFNVIRNGFAWNLRAGFYSYWMPTEYPPFEWDED"
BASE COUNT          538 a         1008 c          948 g          570 t
ORIGIN      
        1 cgcaaggtcg gttcggtcgg tcgccccctg ctcacttcgc acctgcggat ccagggtggc
       61 gagatcctgg ttcagggtcc gaccgtttcg gccggcgccc tcgacgccga cggctggctt
      121 cacactggtg atgtcggctg gatcgacgag gacggcttcc tctacgttca gggccggacc
      181 gacgagatga tcatcaccgg cggtgaaaac gtcattccgg ccgaggtcga ggacgtgctg
      241 gtccagcacc cggagatcct ggaggcggcc gtcatcggcc ggcccgaccc ggaatggcag
      301 caggcggtaa cggcggtcgt ggtcgcccgg gacggggtcg tggtcgacct cgacggcgtg
      361 cggagccact gtgaaggcaa gctcgccccg tacaagatcc cgaaggcaat cgaactggtt
      421 caggagttgc cgaagacccc ctccggcaag cttcaccggg cagccctgct caagtcatag
      481 agccgccccg ctcaagtcat ggagccgctc cgggcgggta acccatagca tcagggtcat
      541 gagcacccaa gacatcttct acgacaagga cggcgacctc tcgaagctcg agggcaagac
      601 cgtcgcgatc ctcggattcg gctcccaggg ccacgcccat gccctgaacc tgaaggattc
      661 gggtgtgaac gtcgtagtcg gccttcgcga aggttccgcc agtcgcgagc aggccgccgc
      721 ggccggcctc gaagtccttt ctgtcgccga cgcctcgagc aagggcgaca tcgtgatgat
      781 cctcctgccg gatgagaagc aggccgaaat ctgggagaac gagatcaaag acgggattgc
      841 cgaaggcaac ctgctgatgt tcgcccacgg tttcgcgatc cgctttgatc agatcgtgcc
      901 gccggccggc gttgacgtcg gcatggtcgc ccccaagggc cccggtcaca tggtccgccg
      961 tcagtttgaa gaaggctcgg gtgttccctg cctgatggcg gttgagcagg acgcaaccgg
     1021 aaacgcccac gacctcgtgc tcgcctatgc gatcggcgtc ggtggcggtc gcgccgggat
     1081 catcgaaacc accttcaagg acgaatgcga aaccgatctc ttcggtgagc aggccgtgct
     1141 ctgcggcggc gccaccgaac tggtccgggc cggtttcgac acgctggtcg aagccggtta
     1201 cgacccgcgc ctggcctact tcgagtgcct gcacgagttg aagctgatcg tcgacctgat
     1261 gtacgagaag gggatccagg gcatgcgcta ctcgatctcc aacaccgccg agtacggtga
     1321 cctgacccgc ggcaagcggg tgatcggcga ggattcacgc gccgcgatga aggagatcct
     1381 cggcgagatc cagtccggcg agttcgccaa ggagttcatc ggcgaaaacc aggccggcaa
     1441 cgcgaacttc gaccgcatgc gccaggaagg caaggatcac aaggtcgaga tcgtgggcaa
     1501 ggagctccgc gacatgatgc cctggatcaa ccaggagttc tgagcacgcg ggcccagagc
     1561 ccaccagccg cccgctggct tcgtcggcgg ccggggaatg acagctcacg tgcccgcgca
     1621 ctatcgcggt cattcccggc ctgtctcctc gccagcaaac ggctggaggg ctctgacctc
     1681 cgcgtcccgg ctttccttct tggcagcgaa cggctggcgg cccctgaact ttgcgctttc
     1741 gaagtctcgt tctcgccagc gaacggctgg agggctctga cctccgcgtc cctggggctt
     1801 cgtgcccgga ttcgttggga ttttcggggc gtttacagga gaacgccgct tcagcgagta
     1861 ggtttcccgt ggccccggtc ccgggggaag acctttagaa ggagtcgtga tgactgatac
     1921 accaaccccc acacctggcg gcgaagaaga gatcgtcgtt tccgaggtga gcgagtccgt
     1981 cgtcgtcccg ggtggctacc cggtggacgt ggacgcccag cttctgcccg agtactcgcg
     2041 gttcatgccg ctgatcaagt ggctgatcct gctgccccac tacatcgtgc tcttcttcct
     2101 cgggatcggc gccatgttcg tggccttcat cgccttcttc gccaccctgt ttacggcgaa
     2161 gtacccggaa ggcatgtgga actacatggt cggcgttcac cgttgggccc tgcgagtgat
     2221 ggcctacaac ttcctgatca ccgacaacta cccgccgttc acgctccagg aaaccgacga
     2281 ggacacgatt cagcttgtgg ccgaataccc ggagcgggtc tcgcgctggc gtcccttctt
     2341 cgccgggctg atctgcattc cgtacgcgat cgtcgcctcg ctgatctaca tggttgcgag
     2401 catctgctcc ttcttcgctt tcttcacgat cctcttcacc aagaagatcc ccgctggcct
     2461 cttcaacgtg atccgcaacg gattcgcctg gaatctccgg gccggcttct actcctactg
     2521 gatgcccacg gagtacccgc ccttcgagtg ggacgaggac tgatcctcgc cgctccagcg
     2581 gtaaccggtt acaccgaaag ggcggcctcc gggccgccct ttcttgtggt tgaagggtct
     2641 ggcccgggcg gcgcccggaa ccaccaggac gacgctgacc ttcgtcaacg gccggaaacg
     2701 gcagctcaag tacccacgta cgatcgcggc catttgcggc cttattcctc agcagccccg
     2761 ccctggaggc ccctggcatc cacccgctga agttgggtgg cgttggcatg tcgcgaaagg
     2821 cagttttctg ccgtataccg gcagaaactt gcctctcgcc gtcttcccga ccccgttccg
     2881 gggctggtgc ctatggattt cgttcatata gacgaaatcc atacccgcct cccgaaaccg
     2941 gggcctgaaa ccggccctgg aggtgccacg ggacgaggga agccccgcga tttccccgtc
     3001 acctgccctg ttccaccaac tgggtcggaa tctcggcagt cgggccggaa atccgacccc
     3061 gcgt
//
LOCUS       DAOQFI010000153         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16062_length_3043_cov_3.774096, whole genome shotgun sequence.
ACCESSION   DAOQFI010000153 DAOQFI010000000
VERSION     DAOQFI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16062_length_3043_cov_3.774096"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLE93_03700"
     CDS             <1..208
                     /locus_tag="PLE93_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86183.1"
                     /translation="RDLETTLLVEFPKRTDPMVRALLANKHEGANPDYEEGNFERELE
                     KRFEVERREELPSGDRILYLARPR"
     gene            240..2366
                     /locus_tag="PLE93_03705"
     CDS             240..2366
                     /locus_tag="PLE93_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH86184.1"
                     /translation="MEPRKGLAAVFDRPAPTGRQLLLGGGHLAALWALAFVQPLLDLL
                     GNNPDFFVARGNSTGDILILAIGFTLLPPLVLFGIEWVVSKVNARAYYGLHLILMGLI
                     ATFFFVPLVSDVFTARSIIILVVSLGLGALLAWMVFRFVFVKNLMDILIIAPIVVLAL
                     FIFNSKTTDLIFPNEGEFKVSADSGNDTPVVLMIFDELGTSNLMTRDGKIAAGRFPNF
                     ARLARSSTWYPNETTTAFFTPHAVPGILTGINQDADTLPTWQEQPLSMFSQFSKGRQL
                     HVLEPVTAICPEDLCPDTEADAGQLTRLKSLFQDLKIVEGKLVLPPGLARKLPDVSSN
                     FEGFGEGSQDEDPVKLGKREHRRGDLAVKAEAKSDPELYEEFIQQVPASDRGFTVMHM
                     HLPHQVWKYDLQGNQYNDSPIEQLSRSLGNWMVNSNGITVSQSRMYVQTGFADRLLGQ
                     MRRKLVKLGLWNKALFIVTADHGISFEGNGVPQRQADKRAMGEVANPPLFIKYPGQKK
                     GVISLKHSMTLDLVPTIGKTLGVGQMYKTDGVPLQGPVPKREVTITDPEGNLFTVGLG
                     EMIRQRRAAIARADARLGTGGVYTLGPAPQLIGRRVAPVPPGTADVLLDEPDIGKDYD
                     PGEDLIPMFITGSWNGSVPAAPKRAPVFAIAVNGRVQATARPFSFDGAVHWGALVNPA
                     SIHQGKNQIGIYRVAGKRLIPLGGNS"
     gene            complement(2515..>3043)
                     /locus_tag="PLE93_03710"
     CDS             complement(2515..>3043)
                     /locus_tag="PLE93_03710"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HNH86185.1"
                     /translation="IRPGTRSFEPVANQDVAPTIIRLAGARAALTIDGCSMIPFWKDP
                     KKRSRRPILLSSYATITRLIPGDYPDDPEQPGDGGPEDRTRRDKDASASVRSPDQNYV
                     GIRLGPYKYIRYESGETELYVLSEDPAELENRAGDPRYLKVINYLETQLADLRGCKGQ
                     SCRSWASRWPSPPGA"
BASE COUNT          539 a         1024 c          975 g          505 t
ORIGIN      
        1 acgggacctg gagaccacgc tcctggtcga gttcccgaag cggaccgacc cgatggtgcg
       61 ggccctcctg gccaacaagc acgaaggagc caaccccgat tacgaggaag gcaacttcga
      121 gcgcgagctg gaaaagcggt tcgaagtgga gcgccgcgag gagctgcctt ccggcgaccg
      181 gatcctctac ctggcccggc cgcggtagga gtttgagcgc tccgggcgaa agttcggaag
      241 tggaaccgag gaagggattg gccgcggtct tcgaccggcc ggccccgacc gggcgtcagt
      301 tgctgctcgg cggaggccat ctggcggcgc tctgggcgct cgccttcgtt cagccgctgc
      361 tcgacctgct gggcaacaac cccgacttct tcgtcgctcg gggcaactcg accggtgaca
      421 tcctgatcct cgcgatcggc ttcaccctgc tcccgccgct ggtcctgttc gggatcgagt
      481 gggtggtcag caaggtcaac gcccgggctt actacggcct gcatctgatc ctgatggggc
      541 tgatcgccac cttcttcttc gttcccctgg tctccgatgt cttcacggcc cggtcgatca
      601 tcatcctggt cgtgtcactg ggccttgggg cgctcctcgc ctggatggtc ttccgcttcg
      661 tcttcgtcaa gaacctgatg gacatcctga tcatcgcgcc gatcgtcgtc ctggccctgt
      721 tcatcttcaa cagcaagacg accgacctga tcttccccaa cgagggcgag ttcaaggtct
      781 cggccgattc cggcaacgac accccggtcg tactgatgat cttcgacgag ctcggcacct
      841 cgaacctgat gaccagggac gggaagatcg ccgccggccg cttccccaac ttcgcccggc
      901 tggcccgcag cagcacctgg tatccgaacg agacgacgac cgccttcttc accccccacg
      961 cggtgccggg aatcctgacc gggatcaacc aggatgccga caccctgccg acctggcagg
     1021 agcagccgct cagcatgttc agccagttca gcaagggccg ccagctccac gtgctggaac
     1081 cggtcactgc aatctgcccg gaggatctct gccctgacac ggaagccgat gccggccagc
     1141 tgacccggct caagtcgctc ttccaggacc tgaagatcgt cgaaggcaag ctggtcctgc
     1201 cgcccggact cgcccgcaag ctgcccgacg ttagttcgaa cttcgagggg ttcggcgaag
     1261 gaagccagga tgaagacccg gtcaagctcg gcaagcgtga acacaggcgc ggggacctgg
     1321 cggtcaaggc cgaggcgaaa tccgatcccg agctgtacga ggaattcatc cagcaggtcc
     1381 ccgcgagcga ccgcggcttc acggtgatgc acatgcacct gccgcatcag gtctggaagt
     1441 acgacctgca gggcaaccag tacaacgact cgccgatcga gcagctctcc cgcagcctcg
     1501 gcaactggat ggtcaactcg aacgggatca cggtctccca gtcacggatg tacgtccaga
     1561 ccggcttcgc cgaccgcctg ctcggtcaga tgcggcgcaa gctggtgaag ctggggctct
     1621 ggaacaaggc cctgttcatc gtcacggccg accacgggat ctccttcgag ggcaacgggg
     1681 tgccgcagcg gcaggccgac aaacgggcga tgggcgaggt cgccaacccc ccgctcttca
     1741 tcaagtaccc cggccagaag aagggcgtga tctccctcaa gcacagcatg accctggacc
     1801 tcgtgccgac gatcggcaag accctgggtg tcgggcagat gtacaagacc gacggggttc
     1861 cgcttcaggg tccggtgccg aaacgggagg tgacgatcac cgacccggag ggcaacctgt
     1921 tcacggtcgg tctcggcgag atgatccggc agcgccgggc ggcgatcgcc agagctgacg
     1981 cacgcctagg aactggcggg gtctacacgc tcgggccggc gccgcagctg atcgggcgaa
     2041 gagtcgcacc ggtgccgccc ggcaccgccg acgtgctgct cgacgaaccc gacatcggca
     2101 aggactacga cccgggcgag gacctgatcc cgatgttcat caccggcagc tggaacggca
     2161 gcgtcccggc cgccccgaag cgggcaccgg tcttcgcgat agccgtcaac ggcagggtcc
     2221 aggccaccgc ccggcccttc agctttgacg gagccgtcca ctggggcgcc ctggtcaacc
     2281 cggcctcgat ccaccagggc aagaaccaaa tcggcatcta ccgcgtcgcc ggcaagcgcc
     2341 tgatccccct cggaggaaac tcttaactgc aagtcgggcg gggtccgacc cgccctccag
     2401 gtaaaacccg ccgacccata gaacaagaga atggacgggt cggctctgtc tgtccctgac
     2461 tcggggaggg gcgggttgaa actggagggc gagcgcagac tcgcccgact gcagttaagc
     2521 ccccggaggg gaaggccacc ggctagccca gctccggcag ctctggccct tgcagccccg
     2581 gagatcggcc agctgggttt cgaggtagtt gatgaccttc aggtagcggg ggtcgccggc
     2641 acggttttcc agttcggccg ggtcctcgct gagtacgtac agctcggttt caccgctctc
     2701 gtaacggatg tacttgtagg ggccgagccg gatcccgacg tagttctggt ccgggctgcg
     2761 gaccgaggcg ctcgcgtcct tgtcgcgcct ggtgcgatcc tccggtcctc catcgccggg
     2821 ctgctccgga tcatccgggt aatcgccggg gatcaggcga gtgatcgtcg cgtagctgga
     2881 aagcaggatc ggccggcgtg accgcttctt cgggtccttc cagaacggaa tcatcgagca
     2941 gccgtcgatc gtcagggcgg cccgggcgcc ggccagcctg atgatggtcg gggcgacatc
     3001 ctggttggcc accggctcga agctcctggt ccccggcctg att
//
LOCUS       DAOQFI010000154         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16067_length_3043_cov_2.800201, whole genome shotgun sequence.
ACCESSION   DAOQFI010000154 DAOQFI010000000
VERSION     DAOQFI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16067_length_3043_cov_2.800201"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLE93_03715"
     CDS             <1..623
                     /locus_tag="PLE93_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNH86186.1"
                     /translation="LKLRARHNLVIRAVAGDGRVSKTRRVTVIGHIRLDRSERYVKLG
                     RKLRVSGVVVPRGVRTVKVRVRGEGVMRTRSRANGRFAFRWTPRRNGDFSYRVWARKS
                     RKSTGDSSRPHRFSALRPGEASYYGPGLYGNGVACGGTLTPTTRGVANKYLPCGTRVT
                     LQYGNRTVVVRVIDRGPYGAGRDWDLTEQTKKDLGFGGVGTVWTNK"
     gene            738..1085
                     /locus_tag="PLE93_03720"
     CDS             738..1085
                     /locus_tag="PLE93_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86187.1"
                     /translation="MSSAESKNLEAISKAIDHHNATCPFPAAEVRMNPFEVERLGWEE
                     IRGLPIVPDPNIGTGRFHIVCSRDMEGDELEEVEAIAEELVTVTPGKRDTPAEPGQEP
                     GEPGIPDNPGRWN"
     gene            1170..2723
                     /locus_tag="PLE93_03725"
     CDS             1170..2723
                     /locus_tag="PLE93_03725"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNH86188.1"
                     /translation="MLLSAAIWTSSAEAGRIAYVADYDANVVSQLDTETNAFLSTSFS
                     GMTDTYMLQVSPDGKYLWIPRFTPNKVSVVDLTDGQALPDIDVDQNPVGVVFSPDSSM
                     AYVLSYDASTVQVFDTATRQQIGDDIPVGAAPYYESLSPDGSRLYVANFDDDTISVIN
                     TADRQVVGDPVPVCADLYSMAISPDGTRLFIGCAGSGDMVMQLSTDTMEATSFSATLP
                     EDPEYLTISPDGATGWAVIRTLDEVVSFSTATGAAIGDPIPVGHEPYHLAITPDGSKA
                     FTSNYEGTQGITPINIATRVAGTLIPDTGGPWQMAITPDQPPSALIAAPISIVAGQPA
                     KFDAVNSIDPDGTISRYDWNFGDGKSKPDGKVTESHVFTKAGQFSVSLILTDNEGCST
                     GFVTAGYTAYCNGGAGASTTLNVRVKAPSNNFSFGRIKLNRKRGTASLQVRLPGPGVV
                     MLAGKNVRGARKTVKKAGKSALSVRITGKAKRALSGRGVVKVKAKVTYRPKYGKPRTK
                     TRTVTLRRN"
     gene            complement(2731..>3043)
                     /locus_tag="PLE93_03730"
     CDS             complement(2731..>3043)
                     /locus_tag="PLE93_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNH86189.1"
                     /translation="CIGPTLGAILSAASTTDSTAKGALLLAVYSAGLAIPFLLTALAF
                     SKMTSAFAVIKRHYGVVIMVGGLVLIAMGILIWTGELSRINIEIQQWSQETGLDFWNS
                     V"
BASE COUNT          586 a          973 c         1001 g          483 t
ORIGIN      
        1 ggttgaagct gcgtgcccgc cacaacctgg tgatccgtgc cgtggcgggt gatggccggg
       61 tttcgaagac ccgtcgcgtc acggtcatcg gccacatcag gctcgaccgc tccgagcgct
      121 acgtgaagct cggccggaag ctccgggtca gcggcgtggt cgtgccacgc ggagttcgca
      181 cggtcaaggt tcgcgttcgc ggtgaaggtg tgatgaggac caggagccgg gccaacggtc
      241 gtttcgcctt ccgctggact ccgcgccgaa acggcgactt cagctaccgg gtctgggcga
      301 gaaagagccg caagtcaacc ggggactcca gccggccgca ccggttcagt gccctgcgtc
      361 cgggcgaagc ctcctattac ggtcctgggc tttacggcaa cggggtcgcc tgtggcggca
      421 cgctcacccc gaccacgcgt ggcgtcgcca acaagtacct gccctgcggc accagggtca
      481 cgcttcagta cggcaaccgc accgtggtcg tccgggtaat cgaccggggg ccgtacggtg
      541 ccggccgcga ctgggacctg accgagcaga ccaagaagga cctcgggttc ggcggggtcg
      601 ggacggtctg gaccaacaaa taaaccaaat cggcgcttgc gcgcctcccg gtgaaacccg
      661 tccgttcaca gaacaaatga tggatgaacg gactccggct tcggttgggc cgggctcact
      721 tcggcataat ccggagcatg agctcggccg aatcgaagaa cctcgaggcg atctcgaagg
      781 cgattgacca tcacaacgcc acctgcccct ttccggcggc cgaggtgcgg atgaatccct
      841 tcgaggtcga gcggctgggc tgggaggaga tccgcggcct gccaatcgtc cccgacccca
      901 acatcggcac cggacgcttc cacatcgtct gctcccgcga catggaaggc gacgaactcg
      961 aagaggtcga ggcgatcgcg gaggagctgg tgacggtcac gcccgggaag agggatacgc
     1021 cggccgaacc gggccaggag ccgggggaac ccgggatccc ggacaacccg gggcgctgga
     1081 actgaacagc tccccttgat ccccggcgga gcgcggacta gtctgctctc atgagatccc
     1141 gggggagggt atgggttttg ctggccggaa tgctgctctc ggcagcgatc tggacctcgt
     1201 cggctgaagc ggggcggatc gcctacgtcg ccgattacga cgcgaacgtc gtgtcgcagc
     1261 tggacacgga aacgaacgcc ttcctgtcca cttcctttag cggaatgacc gatacctaca
     1321 tgctccaggt gtctcccgac gggaagtacc tgtggatccc gaggttcacc ccgaacaagg
     1381 tttcggtggt cgacctgacc gacggccagg cgctgccgga catcgatgtc gatcagaacc
     1441 cggtcggggt ggtcttttcc cccgacagtt cgatggccta cgtgctcagt tacgacgcca
     1501 gcacggtcca ggtcttcgac acggccaccc gccagcagat cggcgatgac attccggtcg
     1561 gggccgcccc ctactacgag tcactctctc cggacggaag ccgtctctac gtcgcgaact
     1621 tcgacgacga cacgatctcg gtcatcaaca ccgccgaccg ccaggtggtc ggcgatccgg
     1681 tcccggtctg cgcggacctg tactcgatgg ccatttcacc ggacgggacc cggctcttca
     1741 tcggctgtgc cggtagcgga gacatggtga tgcagctttc gaccgacacc atggaagcga
     1801 cgagcttctc ggcgaccctg cccgaggatc ccgagtacct gaccatcagt cccgacgggg
     1861 ccaccggctg ggcggtgatc aggacgctcg acgaggtcgt ttccttcagt accgcgaccg
     1921 gggccgcgat cggcgatccg atcccggtcg gccatgagcc gtatcacctg gcgatcaccc
     1981 cggacgggtc gaaggcattc acttcgaact acgagggcac ccagggcatc actccgatca
     2041 acatcgcgac ccgggttgcc ggaacgctga tccccgacac cggcggtccc tggcagatgg
     2101 cgatcacccc cgatcagccg ccgtcggcgt tgatcgcggc gccgatcagc atcgtggccg
     2161 gacagccggc gaagttcgat gccgtcaact cgatcgaccc ggacggcacg atcagccggt
     2221 acgactggaa cttcggtgac ggcaagagca agcccgacgg caaggtgacc gaaagtcacg
     2281 tattcacgaa ggcgggacag ttttccgtgt cgctgatcct gaccgacaac gagggctgct
     2341 ccaccgggtt cgtgaccgcc gggtacaccg cttactgcaa cggcggggcc ggggcatcga
     2401 cgacgctcaa tgtcagggtg aaagcgccat ccaacaactt cagcttcggc aggatcaaac
     2461 tgaaccggaa gcggggaacg gcatcccttc aggtcaggct ccccggcccc ggtgtcgtga
     2521 tgctggccgg aaagaacgtt cgtggtgccc gcaagacggt gaagaaggca ggcaagtctg
     2581 ccctgagcgt tcggatcacg ggcaaggcga agcgggcgct aagcggcagg ggcgtcgtca
     2641 aggtcaaggc caaggtgacc tacaggccga agtacggcaa gccgcgcacc aaaaccagaa
     2701 cggtgaccct gcgccggaac tgatccggcc tcagaccgag ttccagaaat cgagaccggt
     2761 ttcctgggac cactgctgga tctcgatgtt gatgcgggag agctcgccgg tccagatcag
     2821 gattcccatc gcgatcagga ccagaccgcc gaccatgatc acgaccccgt aatgccgttt
     2881 gatcacagcg aaggcgctgg tcatcttcga gaaggcaagg gcggtgagca ggaaggggat
     2941 cgccaggccg gccgaataga cggccagcag cagggcgccc ttggcggttg agtcggtggt
     3001 cgaggcggcg gagagaatcg cgcccagggt gggtccgatg cac
//
LOCUS       DAOQFI010000155         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16102_length_3039_cov_3.350201, whole genome shotgun sequence.
ACCESSION   DAOQFI010000155 DAOQFI010000000
VERSION     DAOQFI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16102_length_3039_cov_3.350201"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..719
                     /locus_tag="PLE93_03735"
     CDS             129..719
                     /locus_tag="PLE93_03735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNH86190.1"
                     /translation="MSDQRGLKMWWQKRRWYERSHRPLSRFRLDLEMARRGSYVRHPI
                     EGDVLGALRDGRLEIGQGCHFEPGCWLTMSDHARVTLGDDVWLNRNVMLAAENEVTIG
                     DHVMFANGCFVGDASHRFNDPTIPITWQGFTSKGPVRIGSNVWLGVNCVVTSGVTIGD
                     HCVIGANSVVTTDLPDNTISAGSPAKVIKEVEFLDH"
     gene            complement(1039..1980)
                     /gene="panB"
                     /locus_tag="PLE93_03740"
     CDS             complement(1039..1980)
                     /gene="panB"
                     /locus_tag="PLE93_03740"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNH86191.1"
                     /translation="MSSGIRKEGEERRPVTLTRLAEMHEAGEPIAMITAYDFPSAQVI
                     EEAGADIVLVGDSAANCVLGYDSTVPVGMDEMTMLAKAVRRGLTTPFLVGDLPFGSYE
                     GSDELAIASAQRLVKEAGCDAVKLEGGGTSVERAKAIIDAGIPVMGHVGLTPQTATAL
                     GGMKAQGRTAERAIKVAEDAIALDQAGCFAVVIEAVPEAVSEMIVAKMTCPTIGIGAG
                     NATSGQVLVLHDLTGINHGHVAKFVKRYSDVRSEMLKGVEAYIAEVRDGSFPAPEHVY
                     SVDPSELEGLQQRLNREDLEHHSYFESTGSWDWEPLS"
     gene            complement(1977..2849)
                     /gene="panC"
                     /locus_tag="PLE93_03745"
     CDS             complement(1977..2849)
                     /gene="panC"
                     /locus_tag="PLE93_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNH86192.1"
                     /translation="MEVIRTPAAMHSLRDELRGQTVTVGLVPTMGYLHEGHNSLLKAA
                     RAENDAVVMSLFVNPTQFKAGEDLSTYPRDEERDLAIAESSGVDFIYAPLPDDVYPSG
                     FATKVEVEGLTEVLCGSETSRGPGHFAGVTTVVTKLLNAVDPDVAYFGQKDAQQATVI
                     RRMAEDLEFRTRIVILPTVRERDGLAMSSRNAYLSPEARERATAIWASLLETESVFSE
                     SGLDAALEAGRRHLQSAGITPEYFEARYPGTLDPAEGTGTDPILVAVAAKVDGTRLID
                     NILIEPSSTSRRNP"
     gene            complement(2840..>3039)
                     /locus_tag="PLE93_03750"
     CDS             complement(2840..>3039)
                     /locus_tag="PLE93_03750"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNH86193.1"
                     /translation="GPPLRRGRGPLTGPIARGDEATVEAHRRALSEQRPELIPFYDAL
                     ADRTREIAGNEAVAEGGRPWR"
BASE COUNT          483 a          980 c         1032 g          544 t
ORIGIN      
        1 tcagcagctt ccgcggcccc ggtttccgcc gcctgatctt cgggttgatc gaccttacgc
       61 ctgagcatca gagaaaacct tgatcgccgt catggccgca cactatccac ctctggcaag
      121 gtattggcat gagcgaccag cgtggactga agatgtggtg gcagaagcgg cgctggtacg
      181 agcgctccca ccggccgctc tcccggttcc ggctcgatct cgagatggcc cgtcgcggct
      241 cctacgtgag gcacccgatc gagggcgacg tgctcggggc gctgcgtgac ggaaggctcg
      301 agatcggcca gggctgccac tttgaaccgg gttgctggct gaccatgtcc gaccacgccc
      361 gggtcacgct tggtgatgac gtctggctca accgcaacgt gatgctggcg gccgaaaacg
      421 aggtcacgat cggtgaccac gtgatgttcg ccaacggctg tttcgtcggc gacgccagcc
      481 accgcttcaa cgacccgacc attccgatca cctggcaggg cttcaccagc aagggaccgg
      541 tcaggatcgg ctccaatgtc tggctcggag tcaactgcgt cgtcacctcc ggcgtaacga
      601 tcggcgatca ctgcgtgatc ggcgccaact ccgtggtcac cactgacctc cctgacaaca
      661 caatctcggc cggctccccg gccaaggtca tcaaggaagt cgaattcctc gaccactgat
      721 tcagcggggt aggcaaaccc gcggaacctt cgtggtccgc cgcggggccg ggtgaggatc
      781 gccgggacgg acgcacgcgc ggcattcagc cgcgctggtc ctggagtgtc ccggacgacc
      841 ctcacccgac cccacggcta ctccaaatcg ggagatgcct cccttcgcgg gtctttggac
      901 tctgaggtgc cgcaggtttg ctaccggaga ggtcgcaaac ctgcggcacc ttcgtgcctg
      961 gaatccgggt tggtgtagcc gcggggtcgg gcgggcatgc ccgcccgacc ccgcggcgtt
     1021 acctccaacg catggagctc aggagagcgg ttcccagtcc caggagccgg ttgactcgaa
     1081 gtaggagtgg tgttcgaggt cttcgcggtt gaggcgctgc tggaggcctt ccagttctga
     1141 ggggtcgacg ctgtagacat gttcgggggc ggggaaggag ccgtcccgga cctcggcgat
     1201 gtaggcctcc acgcccttca gcatctccga acggacatcc gaatagcgct tgacgaactt
     1261 ggctacatgg ccgtgattga tcccggtcag gtcatgcagg acgaggacct ggccggaggt
     1321 tgcgttgccg gccccgatcc cgatcgtcgg gcaggtcatc ttggcgacga tcatctccga
     1381 caccgcctcc ggcaccgctt cgatgaccac cgcgaaacag ccggcctgat cgagcgcgat
     1441 cgcgtcctcg gccaccttga tcgcccgttc ggcggtccgg ccctgggcct tcatgccacc
     1501 gagggcggtc gcggtctgcg gggtgagtcc gacatgaccc atcaccggga ttccggcgtc
     1561 gatgatcgcc ttcgcgcgct cgaccgatgt gccgccgcct tcgagcttga ccgcatcgca
     1621 gccggcttcc ttgaccaggc gctgggcgct ggcgatggca agttcgtccg agccctcata
     1681 ggagccgaag ggcaggtcgc cgaccaggaa aggggtcgta aggccccggc gcacggcctt
     1741 ggccagcatg gtcatttcgt ccatgccgac cggcacggtc gagtcgtagc cgagcacgca
     1801 gttcgcggcc gaatcaccga ccagaacgat gtcggcgccc gcctcctcga tcacctgggc
     1861 cgaggggaag tcataggcgg tgatcatcgc gatcggctcg ccggcctcat gcatttcggc
     1921 cagccgggtc agggttacgg ggcggcgctc ttcgccttct ttgcggattc cggagctcat
     1981 gggttcctcc tggaggtgct tgatggttcg atcaggatgt tgtcgatcag gcgggtgccg
     2041 tccaccttgg cggcgacggc gacgaggatc gggtcggtgc ccgttccttc cgccggatcg
     2101 agggtgcccg gatagcgggc ctcgaagtat tcgggggtga tgccggccga ctggagatgc
     2161 cggcggccgg cttcgagtgc ggcgtcaagg ccggactcgg aaaagaccga ttcggtttcg
     2221 agcagcgagg cccagatcgc ggtggcgcgt tcacgagcct ccggtgagag gtaggcattg
     2281 cgtgaactca tcgccaggcc gtcacgctcg cggacggtgg ggaggatcac gatcctggtg
     2341 cggaactcga ggtcctcggc catgcggcgg atcaccgtgg cctgctgggc atccttctgg
     2401 ccgaagtagg cgacgtcggg atcgaccgca ttgagcagct tcgtgaccac cgtggtcact
     2461 ccggcgaagt ggccggggcc acggctggtt tcactgccgc agagcacttc ggtcaacccc
     2521 tcgacttcga ccttggtcgc gaaaccggag gggtagacgt catccggcag cggtgcgtag
     2581 atgaaatcca cgccggagga ttcggcgatc gccaggtccc gctcctcgtc gcgggggtaa
     2641 gtgctgaggt cctcgccggc cttgaactgg gtcgggttga cgaagaggct catcacgact
     2701 gcgtcattct cggcccgggc cgccttgagc agcgagttgt ggccctcatg gaggtagccc
     2761 atggtcggga ccaggcccac cgtcacggtc tgtccgcgca gctcgtcgcg cagggaatgc
     2821 atcgcggccg gggtgcggat tacctccacg gtcggccgcc ttccgcgacg gcctcgttgc
     2881 cggcgatctc tcgggtgcga tcggccaggg cgtcatagaa ggggatcagc tccggacgct
     2941 gctcgctcag ggcccggcga tgggcttcaa ccgtggcttc atccccgcgg gcgatcgggc
     3001 cggtcagcgg accgcggcca cgtcggaggg gaggaccac
//
LOCUS       DAOQFI010000156         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16123_length_3037_cov_3.486251, whole genome shotgun sequence.
ACCESSION   DAOQFI010000156 DAOQFI010000000
VERSION     DAOQFI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16123_length_3037_cov_3.486251"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1045
                     /locus_tag="PLE93_03755"
     CDS             74..1045
                     /locus_tag="PLE93_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86194.1"
                     /translation="MQTVQTVTGEIAADELGRTLIHEHFYSSDEAVSVQWPHVRDREA
                     EHGLAVESAEAVKAHGVRTVVEPTAMLLGRDIQASRELAEETGLQIIVCTGIYTYDHL
                     PQFLLNRDADYIASLFVHDIEEGIQNTEVKAAFIKCAADEPGLTPNIEKIHRAAARAS
                     LQTGAPIMAHSRPASGTGPKQVEVLLEEGVPAERIQIAHTGDTDDLGYIEGLLDKGVY
                     IGMDRYGLDLFLPTAQRNATVIELLNRGYADRMFLSQDFDIPIANGLDWYPLEVLEQL
                     QAAGAATDWSMTFIFEQVIPALREAGMTGDQLDTMLVENPKRWLGRG"
     gene            complement(1059..1514)
                     /locus_tag="PLE93_03760"
     CDS             complement(1059..1514)
                     /locus_tag="PLE93_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86195.1"
                     /translation="MPSGGPDFYALMPLSGTLASPQQMQEPFSMNDTLTVMQVIPRDG
                     VVSSINGWLYTMSTLNLVGTTISLTGGLYTSEDGFAEPIQQPATLCTASPALTGVVPP
                     GQISTFACDNIGLDVSAGEVGFFAVEANATGVSLNNSLSLRGSMSMVVD"
     gene            complement(1741..2691)
                     /locus_tag="PLE93_03765"
     CDS             complement(1741..2691)
                     /locus_tag="PLE93_03765"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNH86196.1"
                     /translation="MAKILENMEIGHNVMHGQWDWMRDPKIHSTSWEWDNASPADLWK
                     HSHNEIHHTYTNVIGKDHDLGYGIMRVDEDQRWVPFYLGQPAWNLINAFLFEYSIAAY
                     DLQLKKNWKGRRDNPEFQAKAKQVIRKIRAQMTKDYLVHPALSGPSAPTTFAANLTAN
                     LVRNLWTHSVIMCGHFPEGVATFEKASIEGETRADWYVRQMLGAANIKGSPAMHVMTG
                     NLSFQIEHHLFPDLPSNRYQEISPKVREIFDRYDLPYAEGSLPRQVASAWKKVIRLSL
                     PNDSVGKVKSFLGFGRKEKGPSDNVEALPFSDDRFDTMAA"
     gene            complement(2712..2933)
                     /locus_tag="PLE93_03770"
     CDS             complement(2712..2933)
                     /locus_tag="PLE93_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86197.1"
                     /translation="MTAIQKDPENPIGHLSREDIEQIGRELDAIRQEVLDTRGEDDAA
                     YIRGMIDTQRKLELGSRGFCSSRSFRRPG"
BASE COUNT          554 a          960 c          972 g          551 t
ORIGIN      
        1 gacccatcca gcgaccgccc ctgaccgggg cgggagaacc cacaggcaac cgccgaaagc
       61 gaagaggaga ggcatgcaga ccgttcagac cgtcaccggt gagatcgcag ccgacgagct
      121 cggccggacg ctcatccacg agcacttcta ctcatcggac gaggcggttt ccgtccagtg
      181 gccacatgtt cgggaccggg aagccgaaca cgggctggcc gttgaatcag ccgaggcggt
      241 caaggcccac ggcgtgagaa cggtcgtcga gccgaccgcc atgctgctcg gtcgcgacat
      301 ccaggcgagc cgggaactcg ctgaggaaac cggtctccag atcatcgtct gcaccgggat
      361 ctacacctac gaccatctcc cccagttcct gctcaaccgc gatgccgatt acatcgcctc
      421 gctctttgtc catgacatcg aggaaggcat ccagaacacc gaagtcaagg ccgctttcat
      481 caagtgcgcc gccgacgaac ccggcctcac ccccaacatc gagaagatcc accgggcggc
      541 ggcccgggca agccttcaga ccggtgcccc gatcatggcc cacagccgtc ccgcttccgg
      601 gaccggtccg aagcaggtcg aggtccttct ggaggaagga gtgccggccg agcgaatcca
      661 gattgcccac accggtgaca ccgacgacct cggttacatc gagggcctgc tggacaaggg
      721 cgtttacatc ggcatggacc gctacgggct ggacctcttc ctccccaccg ctcaacgcaa
      781 cgcgacggtg atcgaactgc tgaaccgcgg ctacgcggac cgcatgtttc tctcccagga
      841 tttcgacatc ccgatcgcca acggcctcga ctggtacccg ctcgaggtgc tcgaacagct
      901 tcaggcggcc ggcgcagcga ccgactggag catgaccttc atcttcgaac aggtgatccc
      961 ggcgctcagg gaagccggaa tgactggcga tcagctcgac acgatgctgg tcgagaatcc
     1021 gaagcggtgg ctgggtcgtg gctgaggctg accgcgggtc agtccacgac catcgacatc
     1081 gatccccgga gggaaagaga attgttcagc gacaccccgg tggcgtttgc ctcgaccgca
     1141 aagaaaccga cttcgccggc agagacatcg agaccgatgt tgtcgcacgc gaaggtggaa
     1201 atctgaccgg gcggcactac tcccgtaagg gcaggtgaag ccgtgcacaa ggtcgccggc
     1261 tgctgaatcg gctcggcgaa cccgtcttcg ctggtgtaga gccccccggt aagtgaaatg
     1321 gtcgtgccca ccaggttcag ggtcgacatc gtgtagagcc aaccgtttat cgaggagacc
     1381 acgccgtccc tgggaatgac ctgcatgacc gtgagcgtgt cgttcatcga gaacggttcc
     1441 tgcatttgct gcggcgaagc cagggtgccg ctgagcggca tcagggcata gaagtcgggg
     1501 ccgccagagg gcacgcttgt cacaggactc aagggagcgt tcgccgtgaa caccatcgaa
     1561 ccggttgcgg tcccggtcgg gccggtcggg ccgacatcgc cggtcactcc gtgagcgccc
     1621 ggggacccgg tcgctccggt tactccaatt gcgccgggca ggcctgccgg cccgcgagcc
     1681 cggcgaaccc ggtcccctcg gcccggtggc acctctcggc agctggccct tctgtccaga
     1741 tcaggcggcc atggtgtcga acctgtcatc cgagaacgga agcgcctcga cgttgtctga
     1801 agggcccttc tccttcctgc cgaagccaag gaacgatttg actttcccga ccgagtcgtt
     1861 cggaagcgag aggcggatca ccttcttcca ggccgaagcc acctggcggg gcaggctgcc
     1921 ttccgcgtag ggcaggtcgt agcggtcgaa gatctccctg accttcggcg agatctcctg
     1981 gtagcggttg ctgggcagat ccggaaacag gtgatgttcg atctggaagg agaggtttcc
     2041 ggtcattaca tgcatcgccg gagagccctt gatgttcgcc gcgccaagca tctggcggac
     2101 gtaccagtcg gcccgggttt cgccttcgat cgacgccttc tcgaaggtgg ccacaccctc
     2161 cgggaaatgg ccacacatga tcaccgagtg ggtccagagg ttccggacca ggttcgcggt
     2221 caggttcgcg gcgaaggtgg tcggcgcaga gggccccgac aaggccggat ggaccaggta
     2281 gtccttggtc atctgggccc ggatcttcct gatcacctgt ttcgccttgg cttggaattc
     2341 cggattgtcc cggcggccct tccagttctt cttgagctgg aggtcgtagg cggcgatgct
     2401 gtactcgaag aggaaggcgt tgatcaggtt ccaggccggc tggccgaggt agaacggcac
     2461 ccagcgctgg tcctcgtcga cccgcatgat cccgtaaccg aggtcgtggt ccttgccgat
     2521 cacgttcgtg taggtgtgat ggatctcgtt gtgggagtgc ttccagagat cggccggaga
     2581 cgcgttgtcc cactcccagg aggtcgagtg gatcttcggg tcccgcatcc agtcccactg
     2641 gccatgcatc acgttgtggc cgatctccat gttctcgagg atcttggcca ccgaaaggcc
     2701 ggcggtgccg atcagccagg ccggcggaaa gagcgagaag agcagaaccc ccggctaccc
     2761 agttcgagtt tgcgctgcgt gtcgatcatc ccgcggatgt aggcggcgtc atcttcgcca
     2821 cgggtgtcga gcacctcctg gcggatggcg tcaagttcgc ggccgatctg ctcgatgtcc
     2881 tcccgtgaga gatggccgat cgggttttcg ggatccttct ggattgcggt cattgtttcc
     2941 tcagagttcg atttcgcagg tgccggcagc cgctgacacg caggtctgga tgcggatgtc
     3001 gtctccggcc gaggcggtgg tgacttcgcc ggtccgg
//
LOCUS       DAOQFI010000157         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16141_length_3036_cov_2.440792, whole genome shotgun sequence.
ACCESSION   DAOQFI010000157 DAOQFI010000000
VERSION     DAOQFI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16141_length_3036_cov_2.440792"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2090)
                     /locus_tag="PLE93_03775"
     CDS             complement(<1..2090)
                     /locus_tag="PLE93_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNH86198.1"
                     /translation="MNRFRTKVAAALVAALGLLGVAVAQAATGDTLGVTTVTQRIVPD
                     SSAGFNFLAAGPGEAYTVRDGSEEGGVSLGSADAGRATRRTSISYFGQLTDFQLADEE
                     SPLRVEFLDPQGGAFTSAWRPGEAMNPQEEDAMMRQFNAFASKPPNLAKSGAKPKMDF
                     VVNTGDISDNNQYNEALWNLQIAEGGTVNPGTGVDPDPYVGEHPLCPAGMNVLNADDP
                     TAYTGVQDRDEWPAPTMGYFWDPDQPDPGPVAVNPFADWPSYPGLMDRSQRPFKATGL
                     KVPSYFVFGNHDNLVQGNAWGSGIFNQIATGCLKPINDDAANSGRSSNPLLSFVLNPN
                     FTAQDFLDIRTGEPKYFMEVPPDPDRHLISKKEAMNLYRSTGKSNGHGFGFVDQASRT
                     ASNGYASYYSFSPRPGIRYISLDTSSEGGTVLVSDKGNLDDPQFKWLEAQIKKATANN
                     ELVVLFSHHAAGSMTANVPDEAAASCTQVDVAVAPGCDGDPRDSSPIHLGTDVVTMLH
                     NYPNVIAWVAGHSHVNDVTPFPAPDGKSGFWSIRTAALADWPKQNRLVEIFDNRDGNL
                     SIFGTVIDHAAGVDAPASGTSAANMSTDELASISRVVGYNENQSGAEACGSEKCGEGE
                     AEDRNVELVIKDPRKSKAMVNKVTVAPKKRNLKAGKKFKLVVKVTNFITATADAKNVK
                     VTVKSSNKRVKVKNA"
     gene            complement(2296..>3036)
                     /locus_tag="PLE93_03780"
     CDS             complement(2296..>3036)
                     /locus_tag="PLE93_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH86199.1"
                     /translation="PVGWGIRSKVPHKKVGLELGSNAPLIVNEDGDWETAADKAQIHA
                     FSHAGQSCISIQRILVHEAVADRFIERLVANVEKLKVGDPSLDDTDVGPLITPGDRDR
                     VKEWIDEAAAGGAKVLTGGELVDEGRCLAPTLIEGPSREAKVWCEEIFGPVATVDRFS
                     DFDEALARANDSKFGLQAGVFTRDVGNGLKAGNTLEFGGVLINEVPTFRADQQPYGGV
                     KDSGNTREGPAYSIIEMTEERFVTFQAG"
BASE COUNT          476 a          938 c         1036 g          586 t
ORIGIN      
        1 gcgttcttca ccttgacccg cttgttcgat gacttgacgg tcaccttgac gttcttcgcg
       61 tcggcggtgg cggtgatgaa gttggtcacc ttgaccacga gcttgaactt ctttccggcc
      121 ttcaggttcc gcttcttcgg ggccacggtc accttgttga ccatcgcctt cgactttcgc
      181 ggatccttga tcaccagttc gacgttgcgg tcctcggctt cgccttcgcc gcacttctcc
      241 gaaccgcagg cttccgcgcc cgactggttc tcgttgtaac cgaccacccg ggaaatcgag
      301 gcgagttcgt cggtgctcat gttggcggcg ctggtaccgc tcgccggtgc gtcaacgccg
      361 gcggcgtgat cgatcacggt gccgaagatc gagaggttgc cgtcacggtt gtcgaagatc
      421 tcgaccagac ggttctgctt cggccagtcg gccagggccg cggtgcggat gctccagaaa
      481 ccgctcttgc catccggggc cgggaagggc gtcacgtcat tgacgtggct gtggccggcc
      541 acccaggcaa tcacgttcgg gtagttgtgg agcatggtga ccacgtcggt cccgaggtgg
      601 atcggcgagg aatcgcgcgg gtcaccatca caacccggcg ccaccgcgac gtcgacctgg
      661 gtgcaggaag ccgccgcctc gtcggggacg ttggcggtca tgctgccggc ggcgtgatgc
      721 gagaagagca ccaccagttc gttgttggcg gtcgccttct tgatctgggc ttcgagccac
      781 ttgaactgcg ggtcatcgag gttgcccttg tccgagacca gcaccgtgcc gccctcggat
      841 gaggtgtcga gcgagatgta gcggatgccg ggacgggggc tgaacgagta gtaggaggcg
      901 taaccgttcg aggccgtgcg ggaagcctgg tcgacgaaac cgaaaccgtg accgttcgac
      961 ttgccggtgc tccggtagag gttcatcgct tccttcttcg agatcagatg gcggtccgga
     1021 tccggcggga cctccatgaa gtacttgggt tcaccagtcc ggatgtcgag gaaatcctgg
     1081 gcggtgaagt tcgggttcag cacgaagctg agcagcgggt tcgacgaacg gccgctgttg
     1141 gcggcatcgt cgttgatcgg cttcaggcag ccggtcgcga tctggttgaa gatgccgctg
     1201 ccccaggcgt tgccctggac caggttgtcg tgattgccga agacgaagta ggaggggacc
     1261 ttcagtccgg tcgccttgaa gggacgctgg gagcggtcca tcaggcccgg gtaggaaggc
     1321 cagtcggcga aggggttgac ggcaaccggg cccggatcgg gctggtccgg atcccagaag
     1381 taacccatgg tcggggcggg ccactcgtcg cggtcctgca cgccggtgta ggcggtgggg
     1441 tcgtcggcgt tcagcacgtt catgccggcg gggcagagcg gatgctcgcc gacgtacggg
     1501 tccggatcaa ccccggtgcc cgggttcacg gtgccgccct cggcgatctg caggttccag
     1561 agcgcctcgt tgtactggtt gttgtcggag atgtcaccgg tgttgacgac gaagtccatc
     1621 ttcggcttgg cgccgctctt tgcgaggttc ggcggcttgg acgcgaatgc gttgaactgg
     1681 cgcatcatcg cgtcttcctc ctgggggttc atcgcctcgc cgggccgcca ggccgaggtg
     1741 aaggcgcctc cctgcgggtc gaggaactcg acccgcagcg gactctcctc gtcggcgagc
     1801 tggaagtcgg tgagctgccc gaagtaggag atcgaggtgc ggcgggtggc gcggccggcg
     1861 tcggcgctgc cgagcgatac accgccctct tcggagccgt cgcggaccgt gtacgcctcg
     1921 ccgggaccgg cagccaggaa gttgaagccg gcggacgagt cggggacgat ccgctgggtc
     1981 acggttgtca caccgagcgt gtcgcctgtc gctgcctgtg cgactgcaac tccgagcaag
     2041 ccgagggcag ccacaagagc ggcagcaact ttggtcctga accggttcat caattctcct
     2101 tgcggccggt tccccgaacc ggcacatgtg gtgtggggct actcgtccgg tctcctccgg
     2161 acggggacgg aatgagccta cgttaccgcc atgtgaagaa accggccagg agcccggatg
     2221 ttgcgctcac gccggttcgc ttctgggttg aagttattcg cacatgggac gaataacttc
     2281 aacccagtcg cgttctcagc cggcctggaa ggtgacgaag cgttcctcgg tcatctcgat
     2341 gatcgagtag gcgggaccct cgcgggtgtt gccggaatcc ttgacgccgc cataaggctg
     2401 ctggtcggcg cgaaaggtcg gcacctcgtt gattaggacg ccgccgaact cgagggtgtt
     2461 gccggccttc agtccgttgc cgacatcacg ggtgaagaca ccggcctgca ggccgaactt
     2521 cgagtcattg gcccgggcca gcgcctcgtc gaaatcgctg aagcgatcga ccgtggccac
     2581 cggaccgaag atctcctcac accagacctt ggcctcgcgg gaagggccct cgatcagggt
     2641 cggggccagg cagcgcccct cgtcgacaag ctcgccgccg gtcaggacct tcgccccgcc
     2701 ggccgccgcc tcgtcgatcc attccttcac ccggtcccgg tcaccggggg tgatcagtgg
     2761 gcccacgtcg gtgtcgtcca gcgagggatc acccaccttg agcttctcga cgttggcgac
     2821 cagccgctcg atgaaccggt cggccaccgc ttcgtggacg aggatccgct ggatcgaaat
     2881 gcaggactgg cccgcatggg agaaagcgtg gatctgggcc ttgtcggcgg cggtctccca
     2941 gtcaccgtct tcattcacga tcaggggcgc gttcgaaccg agctcgaggc cgaccttctt
     3001 gtgcgggacc ttgctgcgga tgccccagcc gaccgg
//
LOCUS       DAOQFI010000158         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16171_length_3031_cov_3.119624, whole genome shotgun sequence.
ACCESSION   DAOQFI010000158 DAOQFI010000000
VERSION     DAOQFI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16171_length_3031_cov_3.119624"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1237)
                     /locus_tag="PLE93_03785"
     CDS             complement(5..1237)
                     /locus_tag="PLE93_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNH86200.1"
                     /translation="MSHELENEFTDLAQALHESRPTPEPGFTERLDQAVADHFPPEWA
                     DEAALGKRGRGGIGSIAERFRRRTGRRPILLPAMAGFAGLLLVATVVVSAVDSGRFGG
                     GSGGEGDSGVVASKQLDTSASSASESGGISAGSAGTADSATLPVPEASTGAVYGALPS
                     SAMKDQASENSRSSTVPGGSRQRDVALEAEIVLGTDPDGVQDVANEVVSVTDEHNGIV
                     MDSSVTDGEAGNAVASFSLLIPSGQVEEAVADLSGIADLKSRSQELTDITAPTNRTED
                     DVADSKAKIESLLSELEDAPDEATRQELEQKLANERSELSYYESRLKRLERRADYTPV
                     DLRVETGGESSSGGDSSSWGLGDAIDDAGKMLGVAAGVTVLALAVAIPIGIVLLIALA
                     LNRAWVRRSRRRVLADEE"
     gene            complement(1234..1797)
                     /locus_tag="PLE93_03790"
     CDS             complement(1234..1797)
                     /locus_tag="PLE93_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH86201.1"
                     /translation="MEAVPEPVSSPGPKSGPRPLFEDLYRGSRDDLYSYVAGLLRDRV
                     AAEDVTALAFERAFRKWDRYDPKRGSARGWLFGIARNAALDELRKRKREVPAIEPGGL
                     EAPGSVTGAVLEREQRADLMAALGTLAPADREMVALKFFAGLTNTEIAEATGYSSTNV
                     GTRINRAMTRLRSALTDGSASDEEVAR"
     gene            complement(2149..2328)
                     /locus_tag="PLE93_03795"
     CDS             complement(2149..2328)
                     /locus_tag="PLE93_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNH86202.1"
                     /translation="MSTATTQLWICESCGFIYDPAEGDPDGGIPPGTAFEDIPEDWFC
                     PVCGARKTDFEPYEG"
     gene            2432..>3031
                     /locus_tag="PLE93_03800"
     CDS             2432..>3031
                     /locus_tag="PLE93_03800"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNH86203.1"
                     /translation="MNKFPEAGPGESINLGKPSEPRPAATILLLRDREQLGTPEKGVL
                     EVLMLKRSAESHFMPSVWVFPGGSLDPEDGDGLDGLRTCAARELDEEAGIKLDLSSEL
                     IPLARWVTPEVVKTRFDTWFFLARAPGGVEVKPDNFEMTEALWVNPSDALTAAEAGDM
                     AIVFPTLKQLEALAPSANTEEAMGRAAADPNASKVVLPKV"
BASE COUNT          474 a         1016 c         1003 g          538 t
ORIGIN      
        1 aatttcactc ctcgtcagcg aggacgcggc ggcgggagcg acggacccag gcccggttga
       61 gcgccagggc gatcagcaac acgatgccga tcggaatcgc caccgccagc gcgagcaccg
      121 tcaccccggc cgcgacaccg agcatcttgc ccgcatcgtc aatcgcatca ccgagacccc
      181 agctcgacga atcgccgccg gacgagcttt cgccgcccgt ctcgacccgc aagtcgaccg
      241 gggtgtagtc cgcccgccgt tcgagccgct tcagccgtga ttcgtagtag ctcagctccg
      301 agcgttcgtt ggctagcttt tgttccagct cctgccttgt cgcttcatcc ggagcgtctt
      361 cgagctcact cagcaggctc tcgatcttcg ccttcgagtc ggctacgtcg tcctcggttc
      421 ggttggtcgg cgccgtgatg tcggtcagtt cctggctgcg cgacttcaga tcggcgatcc
      481 cggagagatc ggccacggcc tcttcgacct gaccggaagg aatcagcagg ctgaagctcg
      541 cgacggcgtt gccggcctca ccgtcggtga ccgaggagtc catgacgatt ccgttgtgct
      601 cgtcggtcac ggagacgacc tcgttggcca cgtcctgaac cccgtcggga tcggtaccga
      661 gcacgatctc ggcctccaag gcaacatcgc gttggcgaga accgccgggc acggtggaac
      721 tcctcgagtt ctccgatgcc tgatccttca ttgccgagga gggaagggct ccgtacaccg
      781 ccccggtcga ggcctcgggc acaggcaggg tggcggagtc cgcggtaccc gccgaaccgg
      841 ctgagatacc ccccgattcg ctggccgacg atgcggaagt gtccagctgt ttcgacgcaa
      901 caacaccgga gtcgccttca cccccgcttc cgcccccaaa cctgccggag tcgacggcgc
      961 tcaccacgac cgtggcgacc agcaggagtc ctgcgaaccc ggccatcgcc gggagcagga
     1021 tggggcgcct cccggtgcgg cggcggaacc gttccgcgat gctgccgatc cctcccctgc
     1081 ctcgtttgcc gagagcggct tcgtcggccc attcgggcgg gaaatggtcc gcgaccgcct
     1141 ggtcgagacg ctcggtgaag ccgggctccg gggtgggccg ggactcgtgg agagcctggg
     1201 ccaggtcggt gaattcattt tcaagttcgt ggctcatcgg gccacctcct cgtctgatgc
     1261 ggagccatct gtgagtgcgc tccgaagtcg tgtcattgcg cggttgatgc gggtgcccac
     1321 gttggtggac gagtagccgg tcgcttcggc gatctcggtg ttggtcaggc cggcgaagaa
     1381 cttgagggcg accatttcgc ggtcggccgg cgccagggtg ccgagcgccg ccatcaggtc
     1441 ggcgcgctgt tcgcgttcga gcacggcgcc ggtcaccgag cccggggcct ccagccctcc
     1501 aggctcgatc gccggcacct cccgcttgcg tttgcgaagt tcatccaggg ccgcgttccg
     1561 ggcgatcccg aagagccagc cccgggcgct cccccgcttc gggtcgtagc ggtcccactt
     1621 gcgaaaggcc cgttcgaagg cgagcgcggt cacgtcctcc gccgccaccc gatcccgcag
     1681 cagaccagcc acatacgagt agaggtcgtc gcgactgccg cggtaaaggt cctcgaagag
     1741 aggtcgcggc ccgctcttcg gacccgggga cgaaacaggt tcagggacag cttccaacat
     1801 gccgttcata tccagactac gggcggcatc gcgatccgtc acatccccag cctgccgaga
     1861 gtccgcccgc atgcttcgtg ggtcggattt ccggccctgc tgccggttat ccgacccact
     1921 gccggtgata agcggcattg acgggggaat gtggggtcac ccgtagaccc gtggcggctc
     1981 cgaggccggt tcaggccccg gattcgagag gtgccgcaca ttcacgccct ttacgggcct
     2041 gtttgtgcgg cacctctccc gggtcagggt cggtgaaccc tcgagtgacc gacgaaggtc
     2101 agcgtgcggc cggtggatcc cgggtgcgtt gcccgggatc ccgggttatc agccctcgta
     2161 gggttcgaag tcggtcttgc gggcgccgca gacggggcag aaccagtctt cggggatgtc
     2221 ctcgaaggcg gttcccgggg ggatgcctcc gtcggggtcg ccctcggcgg ggtcgtagat
     2281 gaatccgcag gattcgcaga tccagagttg ggtcgtagcg gtgctcatgg cggccagatt
     2341 acccgagagc aggcaggcaa aaccggccgg ccaggtcgcc tcttgtgcgc gcacgcggcg
     2401 ttgtcgccgt tctggcgata cggttcgtga ggtgaacaag tttccggaag ccggaccagg
     2461 ggagtcgatc aatcttggca agccgtcgga gccgcgaccc gctgccacca ttttgttgct
     2521 gcgcgaccgg gagcagctcg gtactcccga aaagggcgtg cttgaggtct tgatgctgaa
     2581 gcgttcggcc gaatcgcact tcatgccgag cgtctgggtt tttccgggcg ggtcgctgga
     2641 tccggaggac ggtgacggac tcgacgggct tcgcacctgc gccgcccggg agctcgacga
     2701 agaggccggc atcaagctcg atctgtccag cgagttgatc cccctcgccc gctgggtcac
     2761 cccggaagtg gtcaagaccc gcttcgacac ctggttcttc ctcgcccgcg cccccggggg
     2821 cgtcgaggtc aaacccgaca atttcgagat gaccgaggca ctctgggtca atccttccga
     2881 cgctctcacg gcagccgagg ccggggacat ggcgatcgtc ttccccactc tgaaacagct
     2941 cgaagcgctt gcgccctcgg cgaacacgga ggaggcgatg ggccgcgctg ccgccgaccc
     3001 caacgcctcg aaggtcgtct tgccaaaggt g
//
LOCUS       DAOQFI010000159         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16224_length_3025_cov_2.649832, whole genome shotgun sequence.
ACCESSION   DAOQFI010000159 DAOQFI010000000
VERSION     DAOQFI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16224_length_3025_cov_2.649832"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLE93_03805"
     CDS             <1..1341
                     /locus_tag="PLE93_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86204.1"
                     /translation="TKYYAFNTDSSRALVMLYKLAGANVKVYRTVAGFTLGGKKFPTG
                     TAILDGSTVTANGINLETYSDTYQTPITGMSVLPSVNRYLIKKPKLAMLTGTTAVTLP
                     PAGRCDIGTTPCQAMFVLREKLGLPVTPVTTTQLAAGELVSGQYTAVVNPATTVAAGA
                     GATALQTFVNNGGSYFSWGTGGATSARNAGMTNVNTTTNGTTDGVPVKVNFNTNSPLS
                     WGFDNGGFLYRVGSGAVTYNPTTLTGNGGSIPNAVEAASYPNPTVRFSYATVAGMTNL
                     FGKTAAVDQAFGAGRVTLLSIDPTFRAWLEGAERLVLNGILYPTGSVITPAGHAAPVT
                     VPAGAIEPGEGEKVTRKELPKVKNRPAKKTHDPANDVRITVGVRQARNLKGAFRAVAP
                     ARIKKIARFSKAKNGNVTLIVPNAHSKDGHFAVWVQKLGARLQANGIQPKAIRL"
     gene            complement(1355..2398)
                     /locus_tag="PLE93_03810"
     CDS             complement(1355..2398)
                     /locus_tag="PLE93_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNH86205.1"
                     /translation="MNDTGVGPLSVEVIPFAMRFRRPYVTAAGVLARRESVLLRLRDE
                     NGLTGLGEGVPMSLRGGDRLELVVAELEAWADDPGLLPASAPARCAVSMAVADLVAKR
                     EQVPLWQYLDPAATPRTLKCNATITSGEVSDVVTQCETWAADGFEVFKLKAGPGEALE
                     LAKAVRSALGHEAKLRIDANGTWGESAGDLLKALEPFGLELVEEPVTGLAELAALSRG
                     TGIPLVADESVNNPEEAADARFEQACEAATVKLSKIGGLDASLGGHLPTYLSSALDGP
                     VGIAAAAHVAQTLDPNLPWPGMAHGLATERLFEQTLSATGPLTALNELDPPANGFGLG
                     VELDEGILAACRL"
     gene            complement(2400..>3025)
                     /gene="menA"
                     /locus_tag="PLE93_03815"
     CDS             complement(2400..>3025)
                     /gene="menA"
                     /locus_tag="PLE93_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HNH86206.1"
                     /translation="QRVLTATWIAFAIAILLGFYIAWRSDWIIIAVGVISIAAGFLYT
                     GGPRPYGYAGLGEVFVFLFFGLVAVNGSYFIQLEELALLPFGLSVSIGFLSTAILVVN
                     NVRDIDSDRRANKNTLAVKIGREKTRRLYVFLVLAAFFVLGLTIVLNDGPWLALIGVI
                     AIPMARDPIAAVTGRTDGPSLNKALAGTGALLGATSILTALGLVLGA"
BASE COUNT          552 a         1027 c          993 g          453 t
ORIGIN      
        1 accaagtact acgccttcaa caccgactcg tcgagggccc tggtcatgct gtacaagctg
       61 gcgggcgcca acgtcaaggt gtaccggacg gtcgccggct tcactctcgg tggcaagaag
      121 ttcccgaccg gcacggcgat cctcgacggt tcgaccgtaa cggcgaacgg gatcaacctg
      181 gagacctaca gcgacaccta ccagacgccg atcaccggca tgtcggtgct gccttcggtc
      241 aaccggtacc tgatcaagaa gccgaagctg gcgatgctga ccggaacaac cgcggtcacg
      301 ctgccgcctg ccggccgctg cgacatcggg accacgccct gccaggcgat gttcgtgctg
      361 cgcgagaagc tgggtctgcc ggtcaccccg gtcacgacca cccagctcgc tgccggcgaa
      421 ctggtcagcg gccagtacac ggcagtcgtc aacccggcca cgaccgttgc ggcgggcgcc
      481 ggagccaccg cgctccagac gttcgtcaac aacggcggca gttacttcag ctggggaacc
      541 ggcggtgcca cttcggctcg taacgccggt atgacgaatg taaacacgac gaccaacggc
      601 accaccgacg gcgttccggt gaaggtgaac ttcaacacca acagcccgct gtcatggggc
      661 ttcgacaacg gtggattcct ctaccgcgtg gggtcgggtg ccgtcaccta caacccgacc
      721 acgctgaccg gcaacggcgg ctcgatcccg aacgccgtcg aagcggcctc gtacccgaac
      781 ccgactgtcc ggttctcgta cgcgaccgtc gccggcatga ccaacttgtt cggcaagacc
      841 gccgcggttg atcaggcctt cggtgccggc cgggtcaccc tgctctcgat cgacccgacc
      901 ttccgggcct ggctcgaggg ggccgagcgc ctggtcctga acggcatcct ctacccgact
      961 ggatcggtca tcacgccggc tggtcacgcg gcgccggtta ccgtgccggc cggtgcgatc
     1021 gagcccggcg agggcgagaa ggtcacccgc aaggagctgc cgaaggtcaa gaaccgcccg
     1081 gcgaagaaga cccacgatcc ggccaatgac gtccggatca cggtgggcgt caggcaggcc
     1141 aggaacctga agggtgcctt ccgcgcggtc gctccggcca ggatcaagaa gatcgccagg
     1201 ttcagcaagg cgaagaacgg caacgtcacc ctgatcgtgc cgaacgccca cagcaaggac
     1261 ggccacttcg ccgtctgggt tcagaagctg ggcgcccggc tacaggccaa cggcatccag
     1321 ccgaaggcga ttcgcctcta ggaagacgcc ggccctagag ccggcaggca gcgagtattc
     1381 cctcgtccag ctcgaccccc aggccaaagc cattggccgg ggggtcgagt tcgttaaggg
     1441 cggtcagcgg gccggtggcc gaaagcgttt gctcgaagag ccgttcggtc gcgaggccgt
     1501 gggccatgcc cggccaggga aggttcggat cgagggtctg ggcgacatgg gccgcggcgg
     1561 cgatgccaac cggtccgtcc agggcgctcg agagataggt gggcaggtgg ccgccaagtg
     1621 atgcgtcgag gccgccgatc ttgctcaatt tgacggtcgc cgcctcgcag gcctgctcga
     1681 atctcgcgtc ggcggcttct tcgggattgt tgaccgattc gtcggcgacc agcgggattc
     1741 cggttcccct gctcaacgcc gcaagttcag ccagtccggt gaccggttcc tcgaccagtt
     1801 cgagcccgaa cggctccagc gccttcagca ggtcccctgc cgactcgccc caggtgccgt
     1861 tggcgtcgat tctgagcttc gcctcgtggc ccagggcgga gcggacggcc ttcgccagct
     1921 ccagcgcttc acccgggccg gccttcagtt tgaagacctc gaatccgtcg gccgcccagg
     1981 tctcgcactg agtgacaacg tcgctcactt cgccggaggt gatcgtcgcg ttgcacttca
     2041 gggttcgcgg tgtggccgcc gggtcaaggt actgccagag cggaacctgc tcgcgtttgg
     2101 cgaccaggtc ggccaccgcc atcgacaccg cacagcgggc cggggcagaa gccgggagga
     2161 gacccgggtc atccgcccag gcttccagtt cggccaccac cagctcgagc cggtcgccac
     2221 cccggaggga catggggaca ccttcgccga gcccggtcag tccgttctcg tcgcgcagcc
     2281 tgagcagcac cgattcccgc cgcgcgagca cgccggccgc ggtcacgtaa ggccggcgaa
     2341 agcgcatcgc gaagggaatc acctcgacgc tgagcggacc gaccccggtg tcgttcattt
     2401 caggccccga gcaccagccc gagcgcggtg aggatcgagg tcgccccgag cagggcgccg
     2461 gtgccggcca gcgccttgtt cagcgaaggc ccgtcggtgc ggcctgtgac tgcggcgatc
     2521 gggtcgcggg ccatcggaat cgcaatcacg ccgatcaggg ccagccacgg tccgtcgttg
     2581 aggacgatgg tcagaccgag cacgaagaac gcggcaagta cgaggaagac atagagccgc
     2641 cgggtcttct cgcgcccgat cttcaccgcc agcgtgttct tgttcgcccg ccggtccgag
     2701 tcgatgtcac ggacgttgtt gacgaccagg atcgcggtgg atagaaaccc gatcgacacc
     2761 gaaaggccga agggcagcag tgccagctcc tcgagctgga tgaagtagga gccgttgacc
     2821 gcaaccaggc caaagaacag gaagacgaag acctcgccca gcccggcgta gccgtacggc
     2881 ctcggcccgc ccgtgtatag gaaccctgcg gcgatcgaga tcaccccgac cgcgatgatg
     2941 atccagtccg aacgccaggc gatgtagaag ccgagcagga ttgcgatcgc aaaggcgatc
     3001 caggtggcgg tcagaacccg ctggg
//
LOCUS       DAOQFI010000160         3015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16301_length_3015_cov_3.404054, whole genome shotgun sequence.
ACCESSION   DAOQFI010000160 DAOQFI010000000
VERSION     DAOQFI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16301_length_3015_cov_3.404054"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLE93_03820"
     CDS             complement(<1..314)
                     /locus_tag="PLE93_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNH86207.1"
                     /translation="MAVETIDEYLDSLPEDRREAIEAVRSAVAAGIPEGYAEELRWGM
                     ISWEIPLETYPETYNGQPLAYVSLASQKNHMALYLMGIYSDEGNRAWFENALKERGTK
                     LD"
     gene            complement(342..1541)
                     /locus_tag="PLE93_03825"
     CDS             complement(342..1541)
                     /locus_tag="PLE93_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH86208.1"
                     /translation="MSSYQEITEDQKAIIEMVHQFVDEQIIPNAEHYDHEDSFPEPIV
                     EQMKELGLFGVTIAEEYGGMGLDLSTYELIVEELSRGWISISGVVNTHFIGVYLLQQF
                     GSEEQKQKYLPKMATGEMRAAFSLSEPEVGSDVQGIKTGAKKDGDAYVIDGQKMWVTN
                     GLGSSLVFLLAKTDPGAEKAHQGMTCFITEKVPWVEENTGEYAGLNVPPKIKKMGYKG
                     VESTELVYNGYRCPAENILGGEEAGLNKGFPQMMDALEVGRVNVAARGVGIAQRALEL
                     ALQYSQERKTFGKPIANHQAIQFKLADMATKLEAARLVTRKAANMKDAGIRSDLEAGM
                     AKLIASETGKECVEECFRIHGGYGYSKEYEIERLYRDAPLLLIGEGTSEIQRMVIGKK
                     LLQRHKI"
     gene            complement(1679..2443)
                     /locus_tag="PLE93_03830"
     CDS             complement(1679..2443)
                     /locus_tag="PLE93_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86209.1"
                     /translation="MAGLLQPVTITNRGSARLTISGPPVITGSNPGDFGVVSNRCSGR
                     ALGFGDSCRVWVAATPQGEGLRVGRLTLPSNSSTVIQADLIVVGTVSPIGATGPTGPT
                     GSSGSTGTTGPTGGTGPTGPTGGTGPTGPTGPTGVTGPRGPAPSVRFAARAFRGLEPG
                     PAAVARVHCPSGSRGCSIFRLRAAWHGVSRAKSLAVIAPRTLRAGKSARIRVVLPARL
                     ARKLNRSVSPGRLAVTVGARTARGRATLVRRSLAVG"
     gene            2637..>3015
                     /locus_tag="PLE93_03835"
     CDS             2637..>3015
                     /locus_tag="PLE93_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNH86210.1"
                     /translation="MRNPRNFFKPKAIGAPDPITQVPFGPSRMIHFFDASNEKMAAKV
                     PDMATQADILLGNLEDAVPVDNKEAARAGLVKVAREADLGGTPLWTRINSLESPWVLD
                     DLTTLVTEVGNKIDVIMVPKVEGP"
BASE COUNT          488 a          992 c          972 g          563 t
ORIGIN      
        1 atatccagct tggtcccgcg ctccttgagg gcgttctcga accaggcccg gttgccttcg
       61 tccgagtaga tgcccatcag gtagagggcc atgtggtttt tctgcgaggc gagcgacacg
      121 taggcgagtg gctggccgtt gtaggtctcc ggataggtct cgagcgggat ctcccagctg
      181 atcattcccc agcggagttc ttccgcgtag ccctcgggaa ttccggccgc tacggccgac
      241 cggaccgctt cgatcgcttc ccggcggtct tcgggaaggg aatcgagata ttcgtcaatt
      301 gtttcgacgg ccatcccgat cccttccctc agcccgtcag atcagatctt gtggcgctgg
      361 agcagcttct tgccgatcac catgcgctgg atttccgacg tgccctcgcc gatcagaagc
      421 agcggggcgt cccggtagag gcgctcgatc tcgtattcct tcgagtagcc gtagccgccg
      481 tggatgcgga agcattcctc gacgcattcc ttgccggttt cggaggcgat cagcttggcc
      541 attccggctt cgaggtcgga acggatgccg gcgtccttca tgttggccgc cttgcgggtg
      601 accaggcggg ccgcttccag cttggtcgcc atgtcggcca gcttgaactg gatcgcctgg
      661 tggttggcga tcggcttgcc gaaggtcttg cgctcctggc tgtactgaag ggcgagctcg
      721 agggcgcgct gggcgatgcc gacgccacgg gcggcgacgt tgacgcggcc gacctcgagg
      781 gcgtccatca tctgcgggaa acccttgttc aggccggctt cctcgccgcc gaggatgttc
      841 tcggccgggc agcggtagcc gttgtaaacc agctcggttg actcgacgcc cttgtagccc
      901 atcttcttga tcttgggcgg aacgttgagg ccggcgtact cgccggtgtt ctcctcgacc
      961 cacggcacct tctcggtgat gaagcaggtc atgccctgat gggccttctc cgcccccggg
     1021 tcggtcttgg cgagcaggaa gacgaggctc gagccgagtc cgttggtgac ccacatcttc
     1081 tggccgtcga tcacgtaggc gtcgccgtcc ttcttggcgc ctgtcttgat gccctggaca
     1141 tcggagccga cctcgggctc ggagagcgag aaggcggcac gcatctcgcc ggtggccatc
     1201 ttcggcagat acttctgctt ctgctcttcg ctaccgaact gctggagcag gtagacgccg
     1261 atgaagtggg tatttaccac accggagatc gagatccagc cgcgggaaag ctcctcgacg
     1321 atcagttcgt aggtcgagag gtcgagcccc atcccgccgt attcctcggc gatggtcact
     1381 ccgaagaggc ccagttcctt catctgctcg acgatcggct ccgggaagga atcctcgtgg
     1441 tcgtagtgct cggcgttggg gatgatctgc tcgtcgacga actgatggac catctcgatg
     1501 atggccttct ggtcctcggt gatctcctga tagctggaca tggctcctct tggggtttgg
     1561 tggcgcagac tgagttgaag cgcgctgtgg cgcggaaaag acccgcgaaa agcacgggca
     1621 aaaaggggca acaaggatat cgggattggc cttctgacgc ggtttccggg agccggccct
     1681 aaccgaccgc gaggctgcgg cggaccagcg ttgcgcgacc cctggcggtg cgcgcaccaa
     1741 ccgttacggc caagcgaccg ggcgagaccg accggttcag tttgcgggcc aggcgagcgg
     1801 gcagcaccac cctgatccgg gcgctcttgc cggccctcag cgtgcgcggc gcgatgaccg
     1861 ccagcgactt cgcacgggag actccgtgcc aggctgccct gaggcggaag atcgaacagc
     1921 cgcggctgcc agacgggcaa tggacccggg ctaccgcggc cggtcccggt tccagtccgc
     1981 ggaatgccct ggcggcaaac ctgaccgacg gggcgggacc ccgcggaccg gtcactcccg
     2041 tcgggccggt gggtccggtc ggcccggtac cgccggtggg tccggtcggc ccggtaccgc
     2101 cggtcggccc ggtggtgccg gtgctgccgc tgcttcccgt tggtcccgtg gggcccgttg
     2161 cgccgatcgg gcttacggtt ccgaccacga tcagatccgc ctggatcacg gtcgaagagt
     2221 tcgagggcag ggtcagcctg cccacccgca gtccctcgcc ctggggcgtc gctgccaccc
     2281 agacccggca ggagtcaccg aagcccaggg cccggccgga acagcggttg gacacgactc
     2341 cgaagtcacc ggggttggaa cctgtaatca ccggtggtcc ggagatcgtc agccgggccg
     2401 aaccacggtt cgtgatcgtg accggctgga gcagacctgc catcgcccct gataaagccc
     2461 ctgatccccg cgtcctttcg tggcacctcg ccaagatata tgtcgcccgc ggaccagtca
     2521 acagcgacat gcgacccaga gggcccgcga gccgcgatcc ctatacgctt ttcggtggcg
     2581 ctgatgctgc ttttcgcgcc gaatagctct tctctaacca accctcaagg agaaccttgc
     2641 gtaacccccg gaacttcttc aagcccaagg cgatcggcgc ccccgacccg atcacccagg
     2701 tccccttcgg cccctcccgg atgatccact tcttcgacgc ctcgaacgag aagatggcgg
     2761 ccaaggtgcc ggacatggca acccaggcgg acatcctgct cggcaacctc gaggatgcgg
     2821 tcccggtcga caacaaggaa gccgcccgcg ccggcctggt caaggtcgcc agggaagccg
     2881 atctcggcgg cacgcccctc tggacccgga tcaactcgct cgagagcccc tgggtgctcg
     2941 atgacctgac caccctggtc accgaagtcg gcaacaagat cgacgtgatc atggtcccga
     3001 aggtcgaagg tccat
//
LOCUS       DAOQFI010000161         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16312_length_3014_cov_3.276445, whole genome shotgun sequence.
ACCESSION   DAOQFI010000161 DAOQFI010000000
VERSION     DAOQFI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16312_length_3014_cov_3.276445"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLE93_03840"
     CDS             <1..486
                     /locus_tag="PLE93_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH86211.1"
                     /translation="TSGVDIGINWMGDKTDLGRFSASLNGTLVLAYDRQFGPLEPFRS
                     NLGVFLNDQVIQRWRHRMSFGWDLGTVGLTLANSYSSGYRDQNTNGAPFNRSPFNDRN
                     VGSYSLWDLSVSYTGVKNLTLSGRAAAEAGATEAPINPRPIAAMATPAVRLLAFLMPR
                     S"
     gene            complement(491..1984)
                     /gene="metG"
                     /locus_tag="PLE93_03845"
     CDS             complement(491..1984)
                     /gene="metG"
                     /locus_tag="PLE93_03845"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNH86212.1"
                     /translation="MSFYVTTPIFYGNGEPHLGHAYSTMAADILARHMRQRGEDVFFL
                     TGTDEHGEPIAQAAEKQGLTPQELVDRSAQSFRDMAAKVDATNDFFIRTTDQGHIERV
                     QAVVDRVKENGFVYEGTYEGWYCPRCADFKNENEIGPGDTCLIHEIPLDRETEQNWFF
                     RLSAFQDELEKLYEENPGMIQPDFRRNEALAFIRGGLNDVSLSRSQISWGVPLSWDPD
                     QVMYVWFDALLNYYTALGYAREGEDLTGRFWPASWHIIAKDILKFHTVYWPAFLMAAG
                     IEVPQRIFIHGFLLMGDKKMSKSLGNVLDPFEVIEKFGADALRFYCFREVSFGQDGSI
                     STEGFETRYESELANDFGNLASRTIAMISRYRDGVVPEAGPDPLLLADFEGITDRVSA
                     LLDDAQIGQALEEIWVLVRRLNRYVEEAQPWVLAKDEADAERLDQVLYSLAEGLRVLT
                     LLLHPYLPASTDILLEALAEPDQGLAPFGSRPGGVKVEKIDPLFPKL"
     gene            complement(2022..>3014)
                     /locus_tag="PLE93_03850"
     CDS             complement(2022..>3014)
                     /locus_tag="PLE93_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86213.1"
                     /translation="GRDRGRLADRDPGQRPLQGPDRPGTAALSAVGSRVAYATCEDRI
                     SDWEDDLLSADLLAERGVEVEFAVWNDPAVDWESFDLTVIRSTWDYTERLEEFLAWCE
                     SVGPGKLRNVPAMILWNTDKRYLAELDGAGLPVPPTALVAPGSNPPPFGGKVVIKPVS
                     GAGARDTGVFDEANEEGGLALLEKLARQDEIAMIQPYIPWIDERGETAVLFFGGRFAY
                     ALKKRAFLPESGVAPVRPGTTVAEGMFAEDLMSLAEASQAEVELGAKVVAWLARRFGS
                     VPLYARIDMVSSPQGEPVLMEVEVTEPSFYLGLTTGLETPGPELFAAAVEAGLG"
BASE COUNT          507 a          980 c          993 g          534 t
ORIGIN      
        1 acctcgggtg tcgacatcgg catcaactgg atgggtgaca agaccgacct gggccggttc
       61 agcgcctcgt tgaacggcac gctcgtcctc gcctacgacc ggcagttcgg gccgctcgag
      121 ccgttccgca gcaacctggg cgtttttctc aacgaccaag tcatccagcg ctggcggcac
      181 cgcatgtcgt tcggatggga cctcggcaca gtggggctga ccctggcgaa cagctactcg
      241 tccggctacc gcgaccagaa caccaacggg gcgccgttca accgttcgcc gttcaatgac
      301 cgcaacgtgg gctcctacag cctgtgggac ctgtcggtgt cctacaccgg agtgaagaac
      361 ctgacgctga gcgggcgcgc ggctgcagag gccggtgcga ctgaagcgcc aatcaatccc
      421 agaccgattg cggcgatggc aactcctgct gtaagactcc tggcttttct catgccccga
      481 tcctagttcc tcagagtttc ggaaacaggg gatcgatctt ttccaccttc acgcccccgg
      541 gccggctgcc aaacggggcc aggccctggt cgggctcggc gagggcttcg agcaggatgt
      601 cggtggaggc cggcaggtag ggatggagca gcagggtgag cacccgcagc ccctcggcca
      661 ggctgtagag aacctggtcg agccgttcgg cgtcggcctc gtccttggcg aggacccagg
      721 gctgcgcctc ctcgacgtag cggttgaggc gacggaccag cacccagatc tcttccaggg
      781 cctgcccgat ctgggcatcg tccagcaggg cggaaacgcg gtcggtgatg ccttcgaaat
      841 ccgccaggag cagcgggtcc ggcccggcct ccggcacgac cccgtcgcgg tagcggctga
      901 tcatcgcgat ggtgcgtgag gcgaggttgc cgaagtcgtt ggccagttcg gactcgtagc
      961 gggtttcgaa accctcggtc gagatcgaac cgtcctggcc gaagctgacc tcgcggaagc
     1021 aatagaagcg cagggcatct gccccgaact tctcgatcac ctcgaacgga tcgagcacgt
     1081 tgccgagcga cttcgacatc ttcttgtcac ccatcagcag gaagccgtgg atgaagatcc
     1141 gctgcggcac ctcgataccg gcagccatca ggaaggccgg ccagtagacg gtgtggaact
     1201 tgaggatgtc cttggcgatg atgtgccagg aggcgggcca gaagcggccg gtcaggtctt
     1261 ctccctcgcg ggcgtagccg agggccgtgt agtagttgag cagggcgtcg aaccagacgt
     1321 acatgacctg gtccgggtcc cagctcagcg gaacgcccca ggagatctgg gagcgggaaa
     1381 gagagacatc gttgagaccg ccccggatga aggcgagcgc ctcattgcgg cggaagtcgg
     1441 gctggatcat tcccgggttc tcttcgtaga gcttctcgag ttcatcctga aaggccgaga
     1501 gcctgaagaa ccagttctgc tcggtttccc ggtcgagcgg gatctcgtgg atcaggcagg
     1561 tgtccccggg gccgatctcg ttctcgttct tgaagtcggc gcagcggggg cagtaccagc
     1621 cctcgtaggt tccctcgtag acgaagccgt tctccttgac ccggtcaacc accgcctgga
     1681 cccgctcgat gtggccctgg tcggtggtgc ggatgaagaa gtcattggtc gcatcgacct
     1741 tcgcggccat gtcgcggaag ctctgggcgc tacggtcgac gagctcctgc ggagtcaggc
     1801 cctgcttctc ggccgcctgc gcgatcggct cgccgtgctc gtcggtcccg gtaaggaaaa
     1861 acacgtcttc accccgctgg cgcatgtgcc gggcgaggat gtcggcggcc atggtcgagt
     1921 aggcgtggcc gagatggggc tcgccgttgc cgtagaagat cggtgtggtg acgtagaaac
     1981 tcacgggaga tccagcatag gacttgtttc gtccgcgtgg ctcagccgag gccggcttcg
     2041 accgccgccg cgaacagttc cggtccgggc gtctcgagtc ccgtggtcag tcccaggtag
     2101 aagctcggct cggtcacctc gacctccatc aggacgggct ccccctgcgg cgagctgacc
     2161 atgtcgatgc gggcgtagag cggtaccgag ccgaatctcc gggccagcca ggcgacgacc
     2221 ttcgcgccca gttctacctc ggcttggctt gcttcggcga ggctcatcag atcctcggcg
     2281 aacatccctt ccgccacggt cgtccccggc cggaccgggg ccaccccgga ttccggcagg
     2341 aaggcgcgct tcttcagggc gtaggcgaag cggccgccga agaacagcac cgccgtctct
     2401 ccccgttcgt cgatccaggg aatgtagggc tggatcattg cgatctcgtc ctgtcgggcg
     2461 agcttctcga gcagggcgag gccaccttcc tcgtttgctt cgtcaaagac tcccgtgtcc
     2521 cgggcgccgg ctccggaaac cggtttgatc accaccttgc cgccgaaggg cggcgggttc
     2581 gagccgggag cgaccagcgc ggtcggcggg accggcagcc cggccccgtc cagttcggcc
     2641 aggtagcgct tgtcggtgtt ccagaggatc atcgccggca cgttgcggag cttccccggc
     2701 cccaccgact cgcaccaggc aaggaattcc tccagccgct ccgtgtaatc ccaggtcgag
     2761 cggattaccg tcaggtcgaa ggattcccaa tcaaccgcgg ggtcattcca gactgcgaat
     2821 tcgacctcca ccccccgttc ggccagcaga tcggccgaga gcaggtcgtc ctcccaatcc
     2881 gaaatcctgt cctcgcaggt ggcgtaggca accctggacc cgacggcgct caacgccgcg
     2941 gttcccggcc ggtcagggcc ttgtagaggt cgttggcccg ggtcccggtc agcgaggcga
     3001 ccacggtcgc ggcc
//
LOCUS       DAOQFI010000162         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16367_length_3008_cov_2.919065, whole genome shotgun sequence.
ACCESSION   DAOQFI010000162 DAOQFI010000000
VERSION     DAOQFI010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16367_length_3008_cov_2.919065"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLE93_03855"
     CDS             <1..733
                     /locus_tag="PLE93_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86214.1"
                     /translation="PYDGPALIDDAAGAIHTRLTIASLITSVGLNLVPERIESVRPRP
                     EGGVEVVSTFGDRVFDSVVVAAGAGTADLAATAGLKVPSQPQAHMRLTLPLAGGVKAP
                     RLACIQDSSGFFGEAAAYGSPVQGNREYAVGLALSTPVDLDADADPLSGLADRTADYV
                     AEAMPGLDRGGAVSFQRWVTELPWAADGLAIWQRGEAFFIAGHNLFKLAPALGSALAD
                     CVENGEVGRHFRPEDKLGEAVLEAR"
     gene            complement(799..1581)
                     /locus_tag="PLE93_03860"
     CDS             complement(799..1581)
                     /locus_tag="PLE93_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HNH86215.1"
                     /translation="MDSKVLNRAETTSSIIGLTDEAELWARRDSDPRASEELVNRYLA
                     FAKSVAIRYRSQSESLDDLVQVASLGLVNAVKRFDPERGVPFIAFASPTITGELKRHF
                     RDRTSAMRLPRSLYDRIGQIDTVVSDLTSQLNREPTVFEIADEMGCPVEEVVEAVEAG
                     HNRQPVSLDQTPGTSEGDQSSPAEWLGFEDSDFETAENRIMLRAAMDELSGEEQMVIQ
                     LRFREELTQSEIAERIGHSQMHVSRMLRRILDRMGDRLPAAA"
     gene            complement(1929..2285)
                     /locus_tag="PLE93_03865"
     CDS             complement(1929..2285)
                     /locus_tag="PLE93_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH86216.1"
                     /translation="MITGLDFIGIPSTDADRSRAFYVDTLGLRPDEGSDYEFWVGESC
                     FGIWEPDKQGMEFSAEKCSHLAFHVEDIEAARAELEKKGVTFFGDTFDTGVCYMAIFA
                     DPDGNSLMLHHRHTPR"
     gene            complement(2326..>3008)
                     /locus_tag="PLE93_03870"
     CDS             complement(2326..>3008)
                     /locus_tag="PLE93_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH86217.1"
                     /translation="RLLDEAREKLIAEVRRVEAGPWEEARVIPSDPLFLGYLILEGAL
                     IREIDVSGRPSIELLGPGDLIRPWVVPQALDALHVNDEWSLLSTGHIAIVDESFHEKA
                     QGYPSILTVLMDRVVARARWLGFQVALSQLTRIDHRLLLTFRYVAERWGVDDPRGVKI
                     PIRLSHRNLAALIGARRPKVSSALTTLSECDLIEQSPDGTWTFFAADIDPMAVLAGID
                     DPPEPIGA"
BASE COUNT          517 a          934 c         1000 g          557 t
ORIGIN      
        1 cccttacgac ggaccggccc tgatcgacga tgcggccggg gcgatccaca cccggctgac
       61 gatcgccagc ctgatcacct cggtcgggct caacctggtc ccggagcgga tcgaatcggt
      121 ccggccccgc ccggaaggcg gggtcgaggt cgtgagcacc ttcggcgacc gggtctttga
      181 ctccgtcgtg gttgctgccg gcgctggtac cgccgacctg gctgcgacgg ccggcctcaa
      241 ggttccttcc cagccgcagg cgcacatgcg cttgaccctg ccgctcgccg gcggagtcaa
      301 ggcaccccgc ctggcatgca ttcaggactc ttcggggttc ttcggcgagg cggccgccta
      361 cggatcaccg gttcagggaa accgtgaata cgccgtaggg ctggccctca gcaccccggt
      421 cgacctggac gctgacgctg accccctctc cgggctggcc gatcgcaccg cggactacgt
      481 ggcggaagcc atgccgggcc tcgaccgcgg cggggccgtt tcatttcagc gatgggtgac
      541 cgagctgccc tgggcagcgg acggtctcgc gatctggcag cggggcgagg cgttcttcat
      601 cgctggccac aatctgttca agctcgctcc cgccctgggc tccgcccttg ccgattgtgt
      661 ggaaaacggc gaggtggggc ggcacttccg gccggaagac aaactcggcg aagcggttct
      721 cgaagctcgc tgattaaaca aaccgaccgg ccagggggcg ggccggtcgg agtgtttcct
      781 ttgggggagt cgggagggtc aggctgcggc cggaagccga tcgcccattc ggtcgaggat
      841 ccgccgcagc atccgggaca catgcatctg ggagtggccg attcgctcgg cgatctcgga
      901 ctgggtcagc tcctcccgga agcgaagctg aatcaccatc tgctcttcgc cgctcagttc
      961 gtccatcgcg gcccggagca tgatgcggtt ctcggccgtc tcgaaatccg aatcttcgaa
     1021 gccaagccat tccgccggtg aactctgatc accctccgag gtgccggggg tctggtcgag
     1081 agaaaccggt tggcggttgt ggccggcctc gacggcttcg accacttcct cgaccgggca
     1141 tcccatctcg tcagcgatct cgaagactgt gggttcgcgg ttgagctggc tggtcaggtc
     1201 tgaaacgaca gtgtcgatct ggccgatccg gtcatagagg ctgcggggca ggcgcatcgc
     1261 cgaggtccgg tcgcgaaagt gacgcttgag ctcgccggtg atggtcgggg aggcgaaggc
     1321 gatgaacggc accccgcgtt ccgggtcaaa ccgcttcacg gcgttgacca ggccgaggct
     1381 ggccacctgg accaggtcgt ccagggattc ggactggctg cggtacctga tcgccacgct
     1441 cttggcgaag gccaggtaac ggttgaccag ttcctccgat gcgcgggggt cgctgtcacg
     1501 gcgggcccag agttcggcct cgtcggtcaa tccgatgatg ctgcttgttg tttccgccct
     1561 gtttagaacc ttgctgtcca tcgctcagcc tctctttctg gaggagtgcc cctccggagt
     1621 tgctgccctg gaaccctctt tttcggggcc gtgcagtggt gcctgatcgt tcggggaaac
     1681 cgtaagcagc gaccggtgaa ctgtccagta tccgaaacct aaagaaagtc ttaagtttcc
     1741 gaacggcctt cagaaagtgg cttcagaatt gggtttcgag cccctgtttt tggcgcaaca
     1801 agtggagaac agcctgtttc acaaaaaaga caacaaagta cgcaagtgtt gcgcccaggg
     1861 cgcggaattg atcgaaaatg tggcctcgac cggccgaaga agcgcgcggc gcagggttcg
     1921 ggcccggctc agcgaggggt gtgccgatgg tggagcatca gcgaattgcc gtcgggatcg
     1981 gcgaagatcg ccatgtaaca gaccccggta tcgaaggtgt ccccgaagaa ggtcacgccc
     2041 ttcttttcaa gttcggccct ggccgcctcg atgtcttcga cgtgaaaggc cagatgggag
     2101 catttctcgg ccgagaactc catgccctgc ttgtccggtt cccagattcc gaagcaggat
     2161 tcgcccaccc agaattcgta gtcggagccc tcgtcggggc gaagtccgag cgtgtccacg
     2221 taaaaggcgc gcgaacggtc ggcgtcggtg gaaggaatgc cgatgaaatc gagtccggtg
     2281 atcatggacc aaaggtatcc ggtcaccggt ccggccgaaa ctgcttcagg cgccgatcgg
     2341 ctccgggggg tcgtcgattc cggccagaac cgccatcggg tcgatgtcgg cggcaaagaa
     2401 ggtccaggta ccgtccgggc tctgctcgat caggtcgcat tcgctcaggg tggtgagagc
     2461 cgagctgacc ttcggccgcc tggcgccgat cagtgcggcc aggtttcggt ggctcagccg
     2521 gattggaatc ttcacgccgc gcgggtcatc gacaccccag cgttcggcca catagcggaa
     2581 ggtgagcagc agccggtgat cgattcgtgt cagctggctc agcgcaacct ggaacccgag
     2641 ccagcgcgcg cgggcgacta cccggtccat cagcacggtg aggatgctcg ggtatccctg
     2701 ggccttttcg tgaaagctct cgtcgacgat cgcgatgtga ccggtggaaa gcagcgacca
     2761 ctcgtcattc acatgcaggg catcgagtgc ctggggcacg acccagggcc ggatcaggtc
     2821 gcccgggccg agcagttcga tcgaaggccg gccggaaacg tcgatctcgc ggatcagggc
     2881 tccctcgagg atcaggtagc cgaggaagag ggggtcggag gggatcactc tcgcttcttc
     2941 ccaggggccg gcttcgaccc gccggacttc ggcgatcagc ttttcgcgcg cctcatccag
     3001 caaccgtc
//
LOCUS       DAOQFI010000163         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16400_length_3004_cov_3.391319, whole genome shotgun sequence.
ACCESSION   DAOQFI010000163 DAOQFI010000000
VERSION     DAOQFI010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16400_length_3004_cov_3.391319"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="PLE93_03875"
     CDS             complement(<1..1564)
                     /locus_tag="PLE93_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNH86218.1"
                     /translation="MAEKKSSGDASYGAKDITVLEGLEAVRKRPGMYIGSTGPRGLHH
                     LVYEIVDNSVDEALAGHNESVEVNLHPDNSCTVTDHGRGIPVDIMEKEGRPAAEVVLT
                     VLHAGGKFGDGGGYKVSGGLHGVGSSVVNALSSWLELRICRDGFVWNQRYERGRPVTE
                     LEKGEATKETGTQITFLPDDEIFEELDFDFQTLLERLRETAFLTRGLRIQITDERAEG
                     QEVEFKYDGGIEDFVRYLNENKEPLGKKVVYFEGEDEKEGQVEVAMQWNASYNDSIHS
                     FANNINTHEGGSHLSGFQAALTRTLNAYARSKGLLKEKDANLAGEDVREGLAAVISVK
                     LHDPQFEGQTKTKLGNPGIKGMVQETVNRKLAEFLEENPAEARLFITKAIEASRARMA
                     ARKARDMTRRGSALENMNLPGKLADCSVRDPELAEIFVVEGDSAGGSAKQARDRSTQA
                     VLPLRGKIINVEKSRIDKVLQNNEIQAMITAIGTGIHDEFDITNARYHKIIMATDADV
                     DGAHIRTLVLTFL"
     gene            complement(1759..2067)
                     /locus_tag="PLE93_03880"
     CDS             complement(1759..2067)
                     /locus_tag="PLE93_03880"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNH86219.1"
                     /translation="MRDARGRSPSPKRLGEVLGDFRGEVTPPTLLAAVQTQWDEVVGP
                     RIAGVTDVIDEREGVVTVECSSAVWAQELELMAPRILARLKDRIDGPVPEKLRFRAAR
                     "
     gene            complement(2064..>3004)
                     /gene="recF"
                     /locus_tag="PLE93_03885"
     CDS             complement(2064..>3004)
                     /gene="recF"
                     /locus_tag="PLE93_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNH86220.1"
                     /translation="AFARCRVTVQGGGVDHVFMASASRSEGNRFTMDGARIEQAEAVL
                     HRPKVTVFSPDRLELVKGPPAGRRAHLDSFVAARWPSRAGLRSTFGRILAQRNALIAR
                     ISSAEANSSQLATWNSQFAEAGANLASGREAAVEALAERWVEAADDLGLEGPKGISYR
                     PGSATTKEEIEAGLEERLEHDLRLGRSSWGPHHDEVRLELDGRQLRRFGSQGQQRLGL
                     LALLFAERAALLDAGEAAPLMLLDDVMSELDAHRRGRLVERLLGGGQSVITAAEGELI
                     PERQEISRVAIRDLVGADEERDNGEDPGAGSGGGTS"
BASE COUNT          457 a         1018 c          957 g          572 t
ORIGIN      
        1 agaggaaggt caggaccagg gtccggatgt gggcgccgtc cacgtcggcg tcggtcgcca
       61 tgatgatctt gtggtagcgg gcattggtga tgtcgaactc gtcgtggatg ccggtgccga
      121 tcgccgtgat catcgcctgg atctcgttgt tctgcaggac cttgtcgatc cggctcttct
      181 cgacgttgat gatctttccg cggagcggga gcaccgcctg ggtcgaacgg tcgcgggcct
      241 gcttggccga accgccggcc gagtcaccct cgaccacgaa gatctcggcg agttccgggt
      301 cacgaaccga gcagtcggcc agcttgccgg gcaggttcat gttctccagg gccgagcccc
      361 ggcgggtcat gtcacgggcc ttgcgggcgg ccatgcgggc ccttgatgcc tcgatcgcct
      421 tggtgatgaa gagtctcgcc tcggccgggt tctcctcgag gaactcggcc agcttgcggt
      481 tgaccgtctc ctggaccatc cccttgattc cggggttgcc cagcttggtc ttggtctggc
      541 cttcgaactg gggatcgtgg agcttgaccg agatcaccgc cgccaggccc tcgcggacat
      601 cctcgccggc caggttcgcg tccttctcct tgagcaggcc cttgctgcgg gcgtaggcgt
      661 tgagtgtccg ggtcagagcg gcctggaaac cggagagatg ggaaccaccc tcgtgggtgt
      721 tgatgttgtt ggcgaagctg tggatcgaat cgttgtagga cgcgttccac tgcatcgcca
      781 cctcgacctg accctctttt tcatcctcgc cctcgaagta cacgaccttc ttgccgagcg
      841 gctccttgtt ttcgttcagg tagcggacga agtcctcgat gccgccgtcg tacttgaact
      901 cgacctcctg gccttcggcc cgttcgtcgg tgatctggat ccgcaggccg cgggtcagga
      961 aggcggtttc acgaagccgt tcgagcaggg tctggaaatc gaagtcgagt tcctcgaaaa
     1021 tctcgtcgtc cggcaggaag gtgatctggg tgcccgtctc tttggtcgcc tctccctttt
     1081 cgagttcggt gaccgggcgg ccgcgctcat aacgctggtt ccagacgaag ccgtcgcgac
     1141 agatccggag ttcgagccag ctcgaaagcg cgttgaccac cgaagagccg acgccatgga
     1201 ggccgcccga aaccttgtac ccgccgccgt cgccgaactt gccgccggcg tgcaggacgg
     1261 tcagcaccac ttcggccgcg gggcgaccct ccttctccat gatgtcgacc ggaatgccgc
     1321 ggccgtggtc ggtgaccgtg cagctgttgt cgggatggag gttgacctcg accgactcgt
     1381 tgtgcccggc cagggcctcg tcgaccgagt tgtcgacgat ctcgtaaacc aggtgatgga
     1441 gaccccgggg tccggtcgag ccgatgtaca tacccgggcg cttgcggacc gcctcgaggc
     1501 cctcgaggac ggtgatgtcc ttggccccgt aggaggcgtc gccgctgctc tttttctcag
     1561 ccaatcagtg cctttccaaa aacttttcgg cggccaccgc cgtaccggct acctgtggag
     1621 aaggtgcgcc cggggggaac cgcgagtcag gtctccataa taccattccc ggtcggataa
     1681 acccgcctgc cagagccaaa aaagccctgc aaaagcgatg tttttgcact tatgtcagtg
     1741 acttgttgca cttcgggctc atctggccgc ccgaaatcgc agtttttcgg gtaccggacc
     1801 gtcgatccga tccttgagcc gggccaggat ccggggcgcc atcagctcca gttcctgggc
     1861 ccagacggca ctcgaacact cgactgtcac gaccccttcg cgctcgtcaa tcacgtcggt
     1921 tacccccgcg atccgggggc ccacgacctc gtcccattgc gtctgaaccg cagccaggag
     1981 ggtcggcggg gtgacctcgc cccggaaatc acccagcact tcccccagcc gcttcgggga
     2041 ggggctcctg ccgcgtgcgt ctctcacgaa gtccctcccc cggacccggc gccggggtcc
     2101 tccccgttgt cccgctcttc atctgcgccg accaggtccc tgatcgcgac ccgggagatc
     2161 tcctgccgct ccgggatcaa ctcgccctcg gccgcggtga tcacgctctg gccacccccg
     2221 agcaggcgct cgaccagacg gcctcgccgg tgggcgtcga gttcgctcat cacgtcgtca
     2281 agcaacatca gcggcgcggc ctcgccggca tcgagcagcg ccgcccgctc ggcgaacagc
     2341 agggcgagga ggcccagccg ctgttgcccc tgggatccga aacgcctgag ctggcggccg
     2401 tcgagctcca gcctcacctc gtcgtggtgc gggccccagc tgcttcgccc gagtcgcagg
     2461 tcatgctcca gccgctcctc gaggccggct tcgatctcct ccttggtggt ggcggatccg
     2521 ggccggtaac tgatcccctt cgggccttcc aggccgaggt catcagccgc ctcgacccag
     2581 cgctcggcca gcgcttcaac cgccgcttcg cgacccgaag caaggttcgc gcccgcttcc
     2641 gcgaactgtg aattccacgt ggcgagttga gaagaattgg cttcagcgga ggaaatgcgg
     2701 gcgatcaggg cattcctctg cgcgaggatc cggccgaaag tggatcgcaa cccggcccgg
     2761 cttggccacc gggcggccac gaaactgtcg aggtgcgccc gccggcccgc cggggggcct
     2821 ttgaccagct ccagccggtc cggcgagaag acggtcacct tcggccggtg gaggaccgct
     2881 tcggcctgct cgatcctggc tccgtccatg gtgaagcgat tgccctccga gcgggaagcc
     2941 gaggccatga agacgtggtc gaccccaccg ccctggacgg tgacccggca ccgggcaaag
     3001 gctg
//
LOCUS       DAOQFI010000164         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16601_length_2984_cov_2.292933, whole genome shotgun sequence.
ACCESSION   DAOQFI010000164 DAOQFI010000000
VERSION     DAOQFI010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16601_length_2984_cov_2.292933"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLE93_03890"
     CDS             <1..305
                     /locus_tag="PLE93_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNH86221.1"
                     /translation="TGPLAIAISTAGASPALAKRMKNEIAAEYGEDHARLAVILNEAR
                     GWAKSTLPTYQDRKVFFEGIVNGEPDPIALIREDRESELLELIENAKREAEASLAA"
     gene            336..1289
                     /gene="rsgA"
                     /locus_tag="PLE93_03895"
     CDS             336..1289
                     /gene="rsgA"
                     /locus_tag="PLE93_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNH86222.1"
                     /translation="MIDPRLAEIGWHKDDQIAWGEVPREDQIPARVVGLHRNHIVDLL
                     TAEGELAGRPAGRMLQDRSSATAMPAVGDWVAALPDGTIQEILPRRSALARRSAADRD
                     RIQILATNIDKAIVISSLNREHDPVRLERMVRLAESSGAEPVIGLSKSDLTDEARELK
                     ERVSQAFPEVKVFVFSSRNGEGVDAVREFLEPGETVVMLGRSGVGKSTLANTLLGWER
                     QKTAEIRGTDDRGRHATTSRELFPLPGGALLIDTPGLRAPGSIEADDLIPGSAESDLT
                     DERAREIERLAAYCRFRDCAHETEPDCAVTAAIEAGELPAR"
     gene            complement(1292..2842)
                     /locus_tag="PLE93_03900"
     CDS             complement(1292..2842)
                     /locus_tag="PLE93_03900"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HNH86223.1"
                     /translation="MNHRRITATVCCLMVALAGAFALAAPASAAYDPNDPEQKAEYDA
                     AFDLGLQAYEFGLPLLDMKRTYLSSTSVNWPSGRGGGPVNMFSHFRKLADARDRTVVL
                     PNSDTLYSMAWLDLSRGPVVIHSRKNTRRFHVFELLDPWTENFANIGSPEGAKPDGDY
                     MVASKWWRGKVPKGLKLIRSPYDRVWVIGRTLVKSKADLKNVWKIQNTYRIVPLKKWN
                     PKRPEAYHPKKPKKLDLVKNEAHIPGTGEGEDPADFFDALGIALARFKAPARDETTLA
                     RMLVGLGIGPGHFPVRNDELSDPQLAALRDVVTGAPSRMQSGLLSTYIQDFDIHNGWL
                     VARTGRYGTDYHRRAQISRFGLGAPTPNVAMYPVALMDRNKAPLTGAKRYVVHFDPDQ
                     AKPPVKFFWSLTLYDNDSFFVDNPIDRYLVNDRSNLKYNDDGSLDIYIQSEAPADPGQ
                     MGNWLPSPAPDAAQPAFRLTVRLYGLSKQGIRNLYSGEGWQLPTILPCGVGNQTSTGV
                     ACATTTPG"
BASE COUNT          504 a          976 c         1016 g          488 t
ORIGIN      
        1 ggaccggccc cctggcgatc gcaatctcga ccgccggtgc ctcccccgca ctcgccaagc
       61 ggatgaagaa cgagatcgcc gccgaatacg gcgaggacca cgcccggctg gcggtgatcc
      121 tcaacgaggc ccgtggctgg gccaagtcga ccctgccgac ctaccaggac cgcaaggtct
      181 tcttcgaagg catcgtcaac ggtgagccgg acccgattgc cctgatccgc gaggaccgcg
      241 agagcgaact tctcgagctg atcgaaaacg ccaagcggga ggcggaggcg tcacttgcgg
      301 cgtagccttc ttcgcatgac cccgggtttc cctgcatgat cgacccccgc ctggccgaga
      361 tcggctggca caaggatgat cagatcgcct ggggcgaggt tccccgggaa gaccagattc
      421 cggcccgggt cgtcggcctt catcgcaacc acatcgtcga cctgctcaca gccgaaggcg
      481 aactggccgg caggccggcg ggccgaatgc tccaggaccg ctcttcggcc accgcgatgc
      541 ctgccgttgg cgactgggtc gccgccctgc cggacgggac gatccaggag atcctgcccc
      601 gccgcagcgc gctggcccgt cgcagcgccg ccgatcgcga ccggatccag atcctcgcca
      661 ccaacatcga caaggcgatc gtgatcagct cacttaacag ggagcatgac ccggtccggc
      721 tggagcgcat ggtccggctg gccgaatcct caggggccga gccggtgatc gggctttcca
      781 aatcggatct gaccgacgaa gcccgcgaac tcaaggaacg ggtcagccag gcgttcccgg
      841 aggtcaaggt cttcgtcttc agttcccgca acggggaagg cgtggacgcg gttcgcgagt
      901 tcctcgagcc gggcgaaacg gtggtgatgc tcggccgttc cggggtcggc aagtcaaccc
      961 tcgcgaacac cctgctcggc tgggagcgcc agaagaccgc cgagatccgc ggcaccgatg
     1021 accgcggccg ccacgccacg accagccgcg aactgtttcc gctacccggc ggggcgctcc
     1081 tgatcgacac gcccggcctc cgtgcccccg gctcgatcga agccgacgac ctgatccccg
     1141 gttcggccga atcggacctc acggacgaaa gggcccgcga gatcgaacgc ctcgccgcct
     1201 actgccgctt ccgcgactgc gcccacgaaa ccgagcccga ctgcgcggtc accgccgcga
     1261 tcgaagcggg cgagctcccc gcccgctgac ctcagcccgg ggtggtggta gcgcaggcga
     1321 cgccggtcga tgtctggttg ccgaccccgc aggggaggat ggtcgggagc tgccagccct
     1381 ctccgctgta gaggttccgg atcccctgct tggaaaggcc gtagaggcgg accgtcaggc
     1441 ggaaggcggg ctgggcggcg tcgggcgccg gagacggcag ccagttgccc atctgaccgg
     1501 gatcggcggg cgcttccgac tggatgtaga tgtccagcga accgtcgtcg ttgtacttga
     1561 ggttcgaacg gtcgttgacc aggtaacggt cgatcgggtt gtcgacgaag aagctgtcat
     1621 tgtcatagag ggtcagcgac cagaagaatt tgaccggcgg cttggcctgg tccgggtcga
     1681 agtggaccac gtagcgcttg gccccggtca gcggggcctt gttgcggtcc atcagcgcga
     1741 ccgggtacat cgccacgttc ggggtcgggg cgccgaggcc gaaccgggag atctgggcgc
     1801 ggcggtgata gtcggttccg tagcgcccgg tccgggcgac gagccagccg ttgtggatat
     1861 cgaaatcctg aatgtaggtg gagagcaggc ccgactgcat gcgggacggc gcgccggtca
     1921 cgacatcacg cagggccgcg agttgcggat ccgagagctc gtcgtttcga accgggaagt
     1981 gaccggggcc gatgcccagg ccgaccagca tccgggccag agtcgtctcg tcccgcgccg
     2041 gcgccttgaa gcgggccagc gcgatgccga gcgcatcgaa gaagtcggcc ggatcctcac
     2101 cctcaccggt tcccggaatg tgggcttcgt tcttgaccag gtcgagcttc ttcggcttct
     2161 tcgggtggta ggcctcgggc cgtttcggat tccacttctt cagcggcacg atccggtagg
     2221 tgttctggat cttccagacg ttcttgaggt cggccttgct cttgaccagg gtgcggccga
     2281 tgacccagac gcggtcgtag ggcgagcgga tcagcttcag acccttcggc accttgcctc
     2341 gccaccactt gctggccacc atgtagtcgc cgtccggttt ggcgccctcg ggcgagccga
     2401 tgttggcgaa gttctcggtc cagggatcaa gcagctcgaa gacgtggaac cgcctggtgt
     2461 tcttgcggga gtggatcacg accggcccgc ggctgaggtc gagccaggcc atcgagtaga
     2521 gagtgtccga attcggcagg acgaccgtgc ggtccctggc gtcggccagc ttgcgaaagt
     2581 ggctgaacat gttgaccgga ccgccgccgc ggccgctcgg ccagttgacc gaggtgctgg
     2641 aaaggtaggt ccgcttcatg tcgagcaggg gcaggccgaa ctcgtaggcc tggagcccga
     2701 gatcgaatgc agcgtcgtac tcggccttct gttcggggtc gttcgggtcg taggcggcgc
     2761 ttgccggagc ggccagggcg aaggcaccag ccagggcgac catgagacaa cagacggttg
     2821 cggtgatgcg gcggtggttc aagacactct cctcaaagcg attccttctc gggaccattc
     2881 ttgcccgaaa agtgatgtac gtcaactaac ccgtccggga gcgaaaagct gcggccccgg
     2941 cccatgccgt taccccggtt cgcctgccgg agaccccgac gcct
//
LOCUS       DAOQFI010000165         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16638_length_2980_cov_2.932308, whole genome shotgun sequence.
ACCESSION   DAOQFI010000165 DAOQFI010000000
VERSION     DAOQFI010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16638_length_2980_cov_2.932308"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..706)
                     /locus_tag="PLE93_03905"
     CDS             complement(197..706)
                     /locus_tag="PLE93_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH86224.1"
                     /translation="MEAIAPRFSRRLTLVLAAVLLAATFAFTTAAPGADARAGSSTAQ
                     TGVLKGWPTKQRLATEFLRLLKNEDTDGLRRFLDPAFLLQRGDGSYLTKSEYLKDPSE
                     VFAFKVRNIVATHNGDVRVLRFEAKTDQIVNGNPVPGVWIPRLSTFKKTGKVWRLIAH
                     ANFLPPVAT"
     gene            799..1788
                     /locus_tag="PLE93_03910"
     CDS             799..1788
                     /locus_tag="PLE93_03910"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNH86225.1"
                     /translation="MSDLRMPAEWAPHEMTLVAWPQREDAWRGTTIEDARDTHAEVVA
                     AIAEFEPVLLVVDPSQAGDARGRVPAENVEIFEAPIDDSWLRDSGPVIVTGEGRRAGI
                     DFTFNSWGEAFLPYDNDAAIAGRILDHLGIEKISSDLVLEGGSIAVDGEGTLVTTGQC
                     LLNPNRNPDLDQAAIEAELASRLDIEMFVWLDQGLIEDEDTDGHVDNICAFIEPGRVL
                     LQTAPEGDPNVPNAQANLARLVDAGLEVVELDLLPRIEGEGDSVVVPYTNFYIANSAL
                     IVPVSGIDPDMDAQALELLGRLYPDREAIGIDGRTLALGGGGIHCITQQIPAA"
     gene            complement(1799..2245)
                     /locus_tag="PLE93_03915"
     CDS             complement(1799..2245)
                     /locus_tag="PLE93_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH86226.1"
                     /translation="MGILEASRVVEIDAPKSECYAIIADLPATPEWQESMISLDVIET
                     NSEGFASLVEIKSDGKVKEVTSLIAFEYQPEEGMSWEQKKGDMKWLKGKWILEELEEG
                     RTRATYWLKADTGRMLGLLIKGPVQDKLKEWLTKDAAEGLKARAES"
     gene            2297..>2980
                     /locus_tag="PLE93_03920"
     CDS             2297..>2980
                     /locus_tag="PLE93_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH86227.1"
                     /translation="MTDLSLNGVHHISCITGDAPGNVEFYAGLLGMRLVKKTVNQDDP
                     SVYHLFYGDEKGSPGFDLTFFEYPGSREGRAGAGMIHRILWRVGSPDSLDYWAARLGQ
                     AGVTTVRSSDEGASFYGIGPTGEGDTLLFQDPEGLTHEILVYDGPDAPLIPGHVDIPA
                     EHAIQGFHGARAFSGNPEVTRQVLGQVLGFTDEGEDHWHLAGEAGDGFEARNGYYFLD
                     EPPADRAIPG"
BASE COUNT          515 a          966 c          976 g          523 t
ORIGIN      
        1 agagcgcttg catggcatgc aagaggtccg cggttcgatc ccgcgtacct ccactcgtaa
       61 gttctcctcg aagtccccgc gtacggcggg cggtccccac ggagccgttc gcgcggctcc
      121 acgggcttcc acgcgcaaag gtgccgcaca tccagccccg tgacggggct gattttgcgg
      181 cacctcggct gttcctctag gtcgcgacgg gcgggaggaa gttggcgtgg gcgatcaggc
      241 gccagacctt gccggtcttc ttgaaggtcg aaagccgggg gatccaaacc cccggaaccg
      301 ggttgccgtt gacgatctgg tcggtcttcg cctcgaagcg aagcaccctc acgtcaccgt
      361 tgtgggtggc gacgatgttg cggaccttga aggcgaagac ttccgaagga tccttgaggt
      421 attcggactt ggtcaggtaa ctgccgtcac cgcgctggag gaggaaagcg gggtcgagga
      481 aacgacggag cccgtcggtg tcctcgttct tcagcagtcg cagaaactcg gtcgcgagcc
      541 gttgcttggt cggccagccc ttcagcacac cggtctgcgc agtcgacgat ccggccctgg
      601 cgtcggcgcc gggcgcagcg gtcgtgaagg cgaaggtcgc ggccagcaga acggctgcga
      661 ggacaagtgt cagtcggcgc gagaagcgcg gggcgatggc ttccatgtgt ctccctgggt
      721 tggtcaagcg aaggtaactg cggcgagcct acccggcggg gtgaaaacag gcaagcgggc
      781 cgggggtaga ctcgcgccat gagcgatctg cgcatgcccg ccgaatgggc gccccacgaa
      841 atgaccctgg tcgcctggcc gcagcgggaa gacgcgtggc ggggaacgac gatcgaggac
      901 gcccgtgaca cccacgcgga agtcgtggcc gcgatcgccg agttcgaacc ggtgttgctg
      961 gtcgtcgacc cctcccaggc gggcgacgcc cgcgggcggg tgccggcgga aaacgtggag
     1021 atcttcgaag ccccgatcga cgattcctgg ctccgggatt ccgggccggt catcgtcacc
     1081 ggcgaaggcc gccgggccgg aatcgacttc accttcaact cctggggtga agcgtttctc
     1141 ccttacgaca acgacgcggc gatcgccggt cgcatcctcg atcacctggg aatcgagaag
     1201 atcagctccg acctggtcct cgagggcgga tccatcgcgg tcgacgggga ggggacgctg
     1261 gtcacgaccg ggcaatgcct gctcaacccg aaccggaatc ccgatctcga ccaggcggcg
     1321 atcgaagcag aactggcttc cagactcgat atcgagatgt tcgtctggct cgaccagggc
     1381 ctgatcgagg acgaagacac cgacgggcac gtcgacaaca tctgcgcctt catcgaaccc
     1441 ggccgggttc tgctccagac cgcgccggaa ggcgatccga acgtgccgaa cgcccaggcc
     1501 aacctcgccc gactcgtcga cgccggactc gaggtggtcg aactcgatct gctcccgcgg
     1561 atcgaaggcg agggcgactc ggtggtggtg ccgtacacga acttctatat cgccaacagc
     1621 gccctgatcg tcccggtcag cggcatcgac ccggacatgg acgcccaggc gctcgagttg
     1681 ctcggtcgcc tctaccctga tcgggaagcg atcgggatcg acggacgcac cctcgccctc
     1741 ggcgggggcg gcatccactg catcacgcag cagatcccgg ccgcctgatc gcggctgtct
     1801 agctctcggc gcgggccttg aggccttcgg ctgcgtcctt ggtcagccat tccttcagct
     1861 tgtcctggac cggacccttg atcagcaggc ccagcatgcg accggtgtcg gccttgagcc
     1921 agtaggtggc gcgggtcctg ccttcctcga gctcctccag gatccacttg cccttcagcc
     1981 atttcatgtc gcccttcttc tgttcccagc tcatgccttc ctcgggctgg tattcgaagg
     2041 cgatcagcga agtgacttcc ttgaccttgc cgtcggactt gatttccacg agcgaggcga
     2101 agccctcgga attcgtctcg atgacgtcaa gcgaaatcat cgattcctgc cactcgggag
     2161 tggcgggaag gtcggcaatg atcgcgtagc actcggactt gggggcgtcg atctccacga
     2221 cgcgtgaagc ttcaaggatt cccatgttcg ggatgctatc ccggaccggg gatgccagcc
     2281 aagtacgctt ggcttcatga ccgatctcag cctgaacggc gttcatcaca tcagctgcat
     2341 caccggcgac gcaccgggaa acgtcgagtt ctacgccggc ctgctcggca tgcggctggt
     2401 caagaagacg gtcaaccagg acgacccctc cgtctaccac ctcttctacg gtgacgagaa
     2461 gggctcgccc ggtttcgacc tgaccttttt cgaatacccc ggatcgcgcg aggggcgggc
     2521 gggagccggg atgattcacc gcattctctg gcgggtcggc tcccctgatt cactcgatta
     2581 ctgggccgcg aggctcgggc aggccggggt cacgacggtc cgcagttccg atgaaggcgc
     2641 ctccttctac gggatcggtc cgaccggtga gggcgacacc ctgttgtttc aggacccgga
     2701 agggctgacc cacgagatcc tcgtctacga cggaccggat gcgccgctga tcccgggcca
     2761 cgtagacatc ccggccgagc atgcgatcca gggcttccac ggagcccgcg ccttctccgg
     2821 caaccccgaa gtgaccaggc aggtgctcgg acaggtgctt ggcttcaccg atgaaggcga
     2881 ggatcactgg catctggccg gtgaggcggg tgacggtttc gaggcccgca acggctacta
     2941 cttcctcgac gagccgccgg cggaccgggc gatcccgggc
//
LOCUS       DAOQFI010000166         2966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16792_length_2966_cov_2.595672, whole genome shotgun sequence.
ACCESSION   DAOQFI010000166 DAOQFI010000000
VERSION     DAOQFI010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16792_length_2966_cov_2.595672"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLE93_03925"
     CDS             complement(<1..1253)
                     /locus_tag="PLE93_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86228.1"
                     /translation="MVAFLTLSAAVLAPTAAARPGDLDPDFGVGGKTVITPASGGMEI
                     PADMAMTPDGGYVVAGADRHAGKSLVVRFDADGNLNTDFGDQGELRTAGNGWQQVEVT
                     ADGHIVLAGGNNSQMVFERLNADGTPDTGFGGDGGVALDVSSLTLPDGATRKAAVSAM
                     RILGDGSIRAIGTGFDCTGEWTDSSCPEALLLGVDQDGSLAAGLGTGGVKDLGIRGWL
                     DAASIFADGSALVIRSHYCDPQYGGALTTNTVSASGIVGPDVNLTGGEDSYYCPDLSS
                     PRRAATIDAQGRVLFVTGQSLWRLNPDGTPDAGFGDQGEAPINDSTRFVDGHPYLKTT
                     GITTDDLGRILLSGGLEDGGKGEGGGDTWASMGAVARLTGKGIVDSSFGDGGVRIVWP
                     GRRAYRKLTSQTPVLTSDDRIVIAGL"
     gene            1430..>2966
                     /locus_tag="PLE93_03930"
     CDS             1430..>2966
                     /locus_tag="PLE93_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86229.1"
                     /translation="MKRLAAEASTRFTSLVATGDEIPFDVAENNGDSSHFYRYVPLTS
                     RFISTHLEELKSLPSYGPARGAIASSDVAAPYLESRGLPVPSEPTRRAEEMIAVFITS
                     LWEGTTEFSLDISRVEAALAGLEVQTRDIREADVLIVPLVGFEMTPTEVTLEGGIRIA
                     QADSIDAPLEATSSEGTGRSAWQTAYFAMTELESTEDGPRQAVNRLNGMIRALRLYKE
                     GSVGLGPFAFAPVGESSWRRIETAVSPPRPGSYFLIESEVDSLNELIRHLAKESPAAD
                     RMEFAQKRFQYGCERENPVEALSDHLLAIRASLGGEGVIDAPLAARAAALITGNADDL
                     RSRERIELALDLEQSLVNGEDLMSIGGESATYIAAWVEDSTRHILREAVLGHYGMNLN
                     IAAEETLITSGLTAGEGSARDYGSSAEWDPVEEPAEDRTTEPVAEEGADIHIFKPRVV
                     GGTSVDGPVDDPLFGIVPEFQAEPLPEAPLQPMPEIERALGIEPSVEPSGEPEFVVSS
                     RGNG"
BASE COUNT          528 a          998 c          925 g          515 t
ORIGIN      
        1 aggcctgcga tcacgatcct gtcgtcactg gtcaaaacgg gtgtctggct ggtcagtttc
       61 ctgtacgccc tgcgaccggg ccagacaatc cggacaccac catcgccgaa ggaactgtcg
      121 acgattccct tgccggtcag tctcgctaca gcgcccatgc tcgcccaggt gtcaccacca
      181 ccctcgccct tgccgccgtc ttcgaggccg ccggaaagga gaatcctccc caggtcgtca
      241 gttgttatcc cggttgtctt tagatatggg tggccgtcca cgaatcgagt ggaatcgttg
      301 atcggagcct caccttgatc gccaaaaccg gcatccggag ttccatcggg attcaggcgc
      361 caaaggcttt gcccggtgac gaagagcacc cgaccctggg cgtcgatcgt tgccgctcgc
      421 cggggtgagg acaagtccgg gcagtaatag ctgtcctcgc caccggtcag gttcacatcc
      481 ggtcccacga tgccgctagc ggagacggtg ttggtggtca gggccccgcc gtactggggg
      541 tcgcagtagt ggctgcggat gaccaaggcg ctgccgtccg cgaagatcga ggcggcgtcg
      601 agccagcctc tgatgccgag gtccttgacg ccgcccgttc cgaggcccgc ggcaagcgat
      661 ccgtcctggt cgactccgag cagcaaagcc tcgggacagg acgaatcggt ccattcaccg
      721 gtgcagtcga agcccgtgcc tatcgccctg atgctcccgt cgccgagaat cctcattgcc
      781 gatacggctg ccttcctggt tgccccgtcg ggcagggtca ggctcgaaac atccagggct
      841 accccgccat ctccgccgaa gccggtgtcg ggggtgccat cagcattcag gcgctcgaag
      901 accatttgcg agttgttgcc accggccagg acgatgtggc cgtcggccgt tacctcaacc
      961 tgttgccagc cgttgccagc tgtcctgagt tcaccctggt cgccgaagtc cgtgttgaga
     1021 ttcccgtccg cgtcgaacct gacgaccagc gatttgcctg cgtggcggtc ggcccccgcc
     1081 accacataac ccccgtcggg ggtcatggcc atgtctgccg ggatctccat gccaccggaa
     1141 gcaggcgtga tgaccgtttt gccgccaaca ccgaaatcgg ggtcgagatc acccgggcgg
     1201 gcggccgccg tgggtgcgag aaccgcagcc gacaaggtca ggaacgcgac caagcctgaa
     1261 agacgcgcga atttgaacaa gacccctcct tggatgaccg ggacaacggc ttgacgctcc
     1321 cataaccgta ttcgaggggg tgagttgcag ttaatcctgc attcgcagga tgcctctgat
     1381 gagaagggaa ctcccaatca ccatgcacga tcctgttctt gatgaggctt tgaagcggct
     1441 cgcagccgag gcatcgacgc ggttcacctc cctggtggcc accggtgacg agatcccctt
     1501 cgacgtggcc gagaacaacg gggacagctc tcacttctac cggtacgtgc cgctcacttc
     1561 acgcttcatc tcgacccacc tcgaagagct gaagtcgctt ccttcatacg gtcccgcgcg
     1621 cggggcgatc gcctcctccg acgtcgccgc tccctatctc gagtcccggg gactgccggt
     1681 tccttccgag ccgacccgcc gggccgaaga gatgatcgcc gtcttcatca cctcgctctg
     1741 ggaaggcaca accgaatttt cgctcgacat cagccgggtc gaagccgctc tggccgggct
     1801 tgaagtccag acccgtgaca ttcgggaggc ggacgtgctg atcgtgccgc tggtcggttt
     1861 cgagatgacc ccgaccgagg tcaccctcga gggcgggatc cggatcgccc aggccgattc
     1921 gatcgacgcg ccgcttgaag caacctccag cgaaggcacc ggccgcagcg cctggcagac
     1981 cgcctacttc gcgatgaccg aactcgaaag caccgaagac gggccgcgcc aggccgtgaa
     2041 ccggctgaac ggcatgatcc gcgcgcttcg cctctacaag gaaggctcgg tcggcctagg
     2101 gcccttcgcc ttcgcgccgg tgggcgagtc gagctggcgc cggatcgaga ccgcggtctc
     2161 gccgccgcgg cccggcagct acttcctgat cgaatcggaa gtcgattccc tgaacgaact
     2221 tatccggcac ctggccaagg agtcgccggc cgccgaccgc atggaatttg cgcagaagcg
     2281 cttccagtac ggctgcgagc gggagaaccc ggttgaggcc ctgagtgatc atctgctggc
     2341 gatccgtgcc tcgctcggcg gggagggcgt gatcgacgct cccctggccg cccgggcggc
     2401 agcgctgatc accggtaatg ccgatgacct gcgctcgcgc gaacggatcg aactcgcgct
     2461 cgacctcgaa cagtcgctgg tcaacgggga agacctgatg tcaatcggtg gcgagtcggc
     2521 cacctacatt gccgcctggg tcgaggattc gacccggcac atcctgcgtg aggcggtgct
     2581 tggccattac ggcatgaacc tcaacatcgc cgccgaggaa accctgatca ccagcggcct
     2641 gaccgccggc gaaggcagcg cccgcgacta cggatcttcg gccgaatggg atccagtcga
     2701 ggaaccggcc gaagaccgga ccacggagcc ggtggccgag gaaggcgccg acatccacat
     2761 cttcaagccg cgcgtggttg gcggaacgag tgtcgatggc ccggttgacg acccgctctt
     2821 cggcatcgtg cccgagttcc aggccgagcc gctgcccgaa gccccgcttc agccgatgcc
     2881 ggagatcgag cgcgcgctcg gaatcgaacc gagtgtggag ccctccggcg aacccgagtt
     2941 cgtcgtgtca tctcgcggca acggca
//
LOCUS       DAOQFI010000167         2953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_16930_length_2953_cov_3.891304, whole genome shotgun sequence.
ACCESSION   DAOQFI010000167 DAOQFI010000000
VERSION     DAOQFI010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16930_length_2953_cov_3.891304"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2030
                     /locus_tag="PLE93_03935"
     CDS             <1..2030
                     /locus_tag="PLE93_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86230.1"
                     /translation="DHVLLARGLPPELQALQRWLDKGYATYSITARGFDESCLSAGSK
                     AADPEGCAKGYIHLIDQRFEIRDTQNFIGKLVDEGLVQPDGLAAWGVSYGGGHTLSFA
                     ALKDRMMNPDGTLVPWTSPDGTPISIKAAVAQVPPTDLPYILAPSGSDLDYIADSSYI
                     YKTRDGRASRVGILKEGWAQGLAATGFTAPIGTDPGADLQGWLADFEKGEPYDGKASI
                     RNSIAELARYHSPYGIDSSEAPAPMFISAGYNDDLVPAVEELRLYNRTRARYPDLPIT
                     LFLGSLGHPRGQIQANVSSALRTKEDQWTDYYLGGTGTRPASEVISYTQTCPNGGDGG
                     GPYTASDWASLSPGEIRLKDAAVQTIGAAGGDPAIAGAWNGVTAGQNPCGPESGSKEP
                     GSANWDLAPAPAGGYTMQGSPTVIADIDLGDSPNSEIAGRLVDLSPDGTTKILVARTI
                     FRPWPSGVQVFQLHPNAWKVEEGHALRLELLAKDAAGAAGSFLINSFRPADGQGDITV
                     RDMELRIPVVETPGALGGLVKAPAKKVLPDRRGVKLAPGYAATGALAIDNPLRAGSKG
                     SVRGKSLKIALTCPASYTNACPNKSVTIKGAPKKGRGRNVKVASASKVKVPGGKTKQV
                     TMKLTAAARKLFRDLKRGKRKVKGLKSLRVAILVDGRQSGYATVRRAGRVR"
     gene            2141..>2953
                     /locus_tag="PLE93_03940"
     CDS             2141..>2953
                     /locus_tag="PLE93_03940"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNH86231.1"
                     /translation="MRKLVLLLALMGSLFAVSAAQAAPPASVFNGAVACTTQGVGDYE
                     GQTWCGSGQYNQNADIRSAVESFDGVPIDVNVAFPDHTQFGDGPYPLVYVFHGYGGGK
                     INFTGMQHWLDKGYAVFSQTNRGFHESCGTATSKADDADCVTKGFVRLDDTRYEVRDA
                     QLFAGMLVDEDLVQPNKIAATGGSYGGGMSMALAALKDRIMLPDGTYAAWTSPQGTPM
                     SIAVAIPDIPWTDLAYSLVPNGSNIDYIEDASYYGPFGIMKQSYVNGLYISGL"
BASE COUNT          539 a         1037 c          931 g          446 t
ORIGIN      
        1 tcgaccacgt gttgctggct cggggcttgc cgcccgaact gcaggccctg cagcgctggc
       61 tcgacaaggg ttacgcgacc tactcgatca ccgcccgcgg cttcgacgag tcctgcctga
      121 gcgccggctc gaaggccgcc gacccggagg gctgcgccaa gggctacatc cacctgatcg
      181 atcagcgctt cgagatccgc gatacccaga acttcatcgg caaactggtt gacgagggac
      241 tggtgcagcc ggacggcctc gctgcctggg gcgtctcgta cggcggcggg cacacgctct
      301 cgttcgccgc gttgaaggac cggatgatga acccggacgg gacgctggtc ccttggacca
      361 gcccggacgg caccccgatc agcatcaagg ccgccgtcgc ccaggttccc ccgaccgatc
      421 tgccctacat cctcgcgccg agcggttccg acctcgacta catcgccgat tcgagctaca
      481 tctacaagac gagggacggc cgggcgagcc gggtcgggat cctcaaggaa ggctgggccc
      541 agggccttgc cgcaaccggt ttcaccgccc cgatcggtac cgatcccggc gccgacctgc
      601 agggctggct ggccgacttt gaaaagggcg agccatacga cgggaaggct tcgatccgga
      661 actcgatcgc cgaactcgcc aggtaccact cgccctatgg aatcgacagc agcgaagccc
      721 cggcccccat gttcatcagc gccggttaca acgacgacct ggttccggcg gtcgaggaac
      781 tccggctcta caaccggacc cgggcccggt acccggacct tccgatcacc ctcttcctcg
      841 gttcgctcgg ccacccgcgc ggccagatcc aggcgaacgt gtcaagtgcg ctgcggacca
      901 aagaggacca gtggaccgac tactacctcg gcggcaccgg aaccaggccg gcgtcggagg
      961 tcatctccta cacccagacc tgccccaacg gcggtgatgg cggcgggccc tacacggcct
     1021 ccgactgggc ttctctttcg cccggcgaga tccggctcaa ggatgcggcc gtgcagacga
     1081 tcggcgccgc cggaggggac ccggcgatcg ccggcgcctg gaacggtgtc actgccgggc
     1141 agaacccctg cggccccgag tccggcagca aggaacccgg atcggccaac tgggatctcg
     1201 ccccggcccc ggcgggcggc tacaccatgc agggctctcc gacggtgatt gccgacatcg
     1261 acctcggtga ttccccgaac tcggagatcg ccggccgcct ggtcgacctc agccccgacg
     1321 gaaccaccaa gatcctggtc gcgcggacga tcttccgccc ctggccgagc ggtgtccagg
     1381 tctttcagct ccaccccaat gcctggaagg tcgaggaagg ccacgccctg cgacttgagc
     1441 tgctggccaa ggacgccgct ggagcggccg gatccttttt gatcaactcc ttccgccctg
     1501 ccgacgggca gggtgacatc acggtcaggg acatggaact gcggatcccg gtcgtcgaga
     1561 cccctggcgc gctcggcggg ctggtcaagg ctccggcgaa gaaggtcctg cccgaccgcc
     1621 ggggcgtgaa gctcgctccc ggctacgcgg cgaccggcgc cctggcgatt gacaacccgc
     1681 tcagggccgg ctcgaagggg tcggtcaggg gcaagtccct gaagatcgcc ctgacctgcc
     1741 cggccagcta caccaatgcc tgcccgaaca aaagcgtgac catcaagggc gcgccgaaga
     1801 agggccgggg caggaacgtg aaggtcgcgt ccgcgagcaa ggtgaaggtc cccggcggca
     1861 agacgaaaca ggtcaccatg aaactgaccg ccgccgcacg caaactgttc cgcgacctca
     1921 agcgaggcaa gcggaaagtc aagggactga agtcgcttcg cgtcgcgatc ctggtcgacg
     1981 gcaggcagtc gggctacgca acggtgagaa gagcgggcag ggtccggtaa acgcgggcgg
     2041 tactgccgtt cgcaacttgt cgcgtaccaa cgcgttctat agagtgactc cgcttcggga
     2101 ttggcccggg gcagggaaaa caggagagaa ccggagaacc atgcgtaagc ttgtattgct
     2161 gctcgccttg atgggcagcc tctttgcagt ttcggccgcc caggcggcac cgcccgcgtc
     2221 cgtcttcaac ggcgccgtcg cctgcacaac ccagggcgtc ggtgactacg aaggccagac
     2281 ctggtgcggc agcggccagt acaaccagaa cgccgacatc cgcagcgccg tcgagtcctt
     2341 cgacggggtt ccgatcgacg tcaacgtcgc cttccccgac cacacccagt ttggcgacgg
     2401 cccctacccg ctggtctacg tcttccacgg ctacggtggc ggcaagatca acttcacggg
     2461 catgcagcac tggctcgaca agggctacgc agtcttctcc cagaccaacc gcggcttcca
     2521 tgagtcctgc ggtaccgcca cctcgaaggc cgatgacgcc gattgcgtga ccaagggctt
     2581 cgtgcgtctc gacgacaccc gttacgaagt ccgcgacgcg cagctcttcg ccggcatgct
     2641 ggtcgacgag gacctggtcc agccgaacaa gatcgccgcg accggcggca gctacggcgg
     2701 cggcatgtcg atggccctgg cggcgctgaa ggaccgcatc atgctcccgg acggcaccta
     2761 cgccgcctgg accagcccgc agggcactcc gatgagcatc gcggttgcga ttccggacat
     2821 cccgtggacc gaccttgcct actccctggt tcccaacggc agcaacatcg actacatcga
     2881 ggacgcctcc tactacggcc cgttcggcat catgaagcag tcctacgtga atggcctcta
     2941 catcagcggc ctt
//
LOCUS       DAOQFI010000168         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17029_length_2944_cov_3.182762, whole genome shotgun sequence.
ACCESSION   DAOQFI010000168 DAOQFI010000000
VERSION     DAOQFI010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17029_length_2944_cov_3.182762"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1041
                     /locus_tag="PLE93_03945"
     CDS             202..1041
                     /locus_tag="PLE93_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86232.1"
                     /translation="MARAQNNIQQAKPAAPGGPLWLLRRFGWFIEKYLLWPIADSFRA
                     IGRGIARIPNAFRYRSPMAYIGVTLMLTLTAGAIAAAVYFYKQQEDKGQDPAVATTAV
                     PTETVVPSVPTPTTTPTTVEDTTPNSGDVDQTLQGVVPNFQTSGKTKQSGAGADSPAG
                     KLPGTVVRPSPVPDSPPLKVAHRFATIFANYEVGGKGAAKQFSSVATAKLARELRENP
                     PRLPSNGQIPKATVVNVVAGKRSGDSLDASVSLMRSGAASELRLDLVREKGKGWLVDE
                     VRG"
     gene            1051..2796
                     /locus_tag="PLE93_03950"
     CDS             1051..2796
                     /locus_tag="PLE93_03950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNH86233.1"
                     /translation="MGRFRTALATISVGALLATGHAASAESATPITDEPATTAGIGPI
                     GPTGTSGSTGSTGNTGNTGNTGNTGNTGSTGNPGNNGNSGNGNTGGVDPDGNTGGNQN
                     QGSGNNGNQTPNPQTENPAKAEEIIPNSDSPLSATITTSPGLGLSGDSTPVAACDGSA
                     GPPRNLVPIYVEAAKKYGLGEQGPQILAAINSIETDFGRLNNVTSYAGAIGWMQFMPS
                     TWDMYATDGDGDGKTDPYNPHDAIHAAARYLQAAGAPGDWYDAIYAYNHADWYVQDVL
                     DKAACYGALGDTIDAEEEHRNLFCEPSEARALEIPNYYMRGFEDAASRYDLGKRGVWV
                     LAAVARLESDYGRGMTAKQLKDTGPMGLSRDEWKQYGVDGDGDGYVERDEPWDGIATF
                     ARMLWSQPTLEAGLFEHNHATWYVEAAVQDAGNIAGDCQTVKTDWNISYPITTNATEI
                     NWDNLQVLNPAAQEDIQNGLIDQRVINLLAILTQKYSLLISALRSDHSQMTASGNVSN
                     HYYGRAMDIAAVNGVPCTDQSADSPCTEAIMLLASLPDGVKPTELIYGWDVDGSGPAF
                     ALPDHTNHIHAGFGP"
BASE COUNT          565 a         1025 c          903 g          451 t
ORIGIN      
        1 tttcttgatc aagatgatat gaaatgtggg tggagagtcg aaatcggctc tccatctacg
       61 ttttatgcga gacacgggtt gcactttcgc acccgcttaa ccgcttcttt acttcctctt
      121 ttgggttccc gtattaccct gcacgctccg cgtctggcct cagggaggcg tatcggaggc
      181 ggaaagcagg ccccggaccc gatggcgaga gctcaaaaca acatccagca ggcgaagcca
      241 gccgctcccg gcgggccgct ttggctgctc cggcgcttcg gctggtttat cgagaagtac
      301 cttctctggc cgatcgcgga ctccttccgt gcgatcggcc gagggattgc ccggatcccc
      361 aatgctttcc gctaccgcag cccgatggcc tacatcggcg tcaccctgat gctcaccttg
      421 accgccggtg cgatcgctgc cgccgtctac ttctacaagc agcaggagga caagggccag
      481 gatcccgccg tcgccaccac cgcggtgccg accgagacgg tcgttccctc ggttccgacg
      541 ccgaccacca ccccgaccac ggtcgaagac acaaccccga actcgggtga tgtcgaccag
      601 acccttcagg gcgtggtccc gaacttccag acttctggca agacgaaaca aagcggtgcc
      661 ggcgcagata gcccggccgg gaagcttccg gggaccgtgg tccggccgtc ccccgtcccc
      721 gactccccgc cgctcaaggt cgcccacagg ttcgccacga tcttcgccaa ctacgaggtt
      781 ggcggcaagg gtgcggccaa acagttttct tccgtcgcca ccgcgaagct ggcccgcgaa
      841 ctgcgggaga acccgccgcg gttgccgtca aacggccaga tccccaaggc gacagtggtc
      901 aacgtggtcg ccggcaagag gtccggcgac tcgcttgacg cgagcgtctc tctgatgcgc
      961 tccggtgcgg cctccgaact gcggctcgac ctggtccggg agaagggcaa gggctggctg
     1021 gtggacgagg tacggggata ggcggaaaga gtggggaggt tcagaacagc tctggcgacg
     1081 atctcggtcg gcgccctgct cgccaccggc cacgccgcca gtgcagaaag cgcgacgccg
     1141 attaccgacg agcccgccac caccgccggc atcggtccga tcgggccgac cggcaccagc
     1201 ggatcgaccg gttccaccgg caatacaggc aataccggga acaccggcaa tacggggaac
     1261 accggcagca ccgggaatcc gggcaacaac ggtaactccg gcaacggcaa caccgggggc
     1321 gtcgatcccg acggcaacac cggcggcaac cagaatcagg gctccggcaa caacggcaac
     1381 cagacgccga acccccagac ggaaaacccg gcgaaggctg aagagatcat cccgaacagc
     1441 gactcgccgc tttcggcgac catcacgacc agccccggcc tcggcctctc tggtgattcc
     1501 actccggtcg ccgcctgcga cggcagcgcc ggcccgcccc gcaacctggt cccgatctac
     1561 gtcgaggccg ccaagaagta cggtctcggc gagcagggcc cgcagatcct cgccgcgatc
     1621 aattcgatcg agaccgactt cggccgcctc aacaacgtga cttcctacgc cggggcgatc
     1681 ggctggatgc agttcatgcc ctcgacctgg gacatgtacg cgaccgacgg tgacggcgac
     1741 ggcaagaccg acccctacaa cccgcatgat gcgatccatg ccgccgcccg ctacctgcag
     1801 gcggcgggcg cgccgggcga ctggtacgac gcgatttacg cctacaacca cgccgactgg
     1861 tacgtccagg atgtgctcga caaggccgcc tgttacggcg cactcggaga cacgatcgat
     1921 gccgaagagg agcacaggaa cctgttctgc gagccctccg aggcgcgggc cctggaaatc
     1981 ccgaactact acatgcgtgg cttcgaggac gcggcctcga ggtacgacct cggcaagcgc
     2041 ggcgtctggg tgctggccgc agtggcccgg ctcgaatccg actatggccg cggcatgacc
     2101 gcgaagcagt tgaaggacac cggtccgatg ggtctttccc gcgacgagtg gaagcagtac
     2161 ggggtcgacg gtgacggtga cgggtacgtc gagcgggacg agccctggga cgggatcgcg
     2221 accttcgccc ggatgctctg gtcacagccg acgctcgagg ccggactctt cgaacacaac
     2281 cacgccacct ggtacgtcga ggcggcggtc caggatgccg gcaacatcgc cggcgactgc
     2341 cagaccgtca agaccgactg gaacatctcc tatccgatca ccaccaacgc gaccgagatc
     2401 aactgggaca acctgcaggt cctcaacccg gctgcccagg aagacatcca gaacggcctg
     2461 atcgaccagc gggttatcaa cctcctcgcg atcctgaccc agaagtacag cctgctgatc
     2521 agcgccctgc gctccgatca cagccagatg accgcctccg gcaacgtctc gaaccactat
     2581 tacggacggg ccatggacat cgccgcggta aacggcgtcc cctgcaccga ccagtcggcc
     2641 gacagcccct gcaccgaggc gatcatgctg ctggcctccc tgcccgacgg ggtcaagccg
     2701 accgagctga tctacggctg ggatgtggac ggatccggac cggccttcgc gctcccggac
     2761 cacaccaacc acatccacgc cggcttcggc ccctgagttc acttcccctc gatttctttg
     2821 gcgtaggatc ttctccagtc caaccaggga ggttcctttg agtgagaaga tttctcgtcg
     2881 gcggctggtt caggccgggg ccgtcgcggc ggccggggcg gcactgccgg cggctggtgc
     2941 cgaa
//
LOCUS       DAOQFI010000169         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17085_length_2939_cov_3.357143, whole genome shotgun sequence.
ACCESSION   DAOQFI010000169 DAOQFI010000000
VERSION     DAOQFI010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17085_length_2939_cov_3.357143"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PLE93_03955"
     CDS             <1..1099
                     /locus_tag="PLE93_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86234.1"
                     /translation="GSNNNPTVTPDTLASAMDEVCTDTNADFEAMGTRGLTMDGIAAE
                     FEGYAEVRQAVVDGFNELNTDDQAEPQLQKYIDASEQVIAQDKAIAKAAAEGDQKAMN
                     EAFAAQTKAFNERDKVAKEIGTEVCGQTPEIKVEKTGTAPPSDLNVVEPKNTVEDAAD
                     NYIEATASGNCGLINANRHTDAGDLDEASCKAAGQALKGARVIGTESYGPVAQAEILS
                     GNGTHYPTYFVTDLDGNLRYGGDAIHDSGGLRPAPEGNDAEATVEATVNAIRDNDAAA
                     FNETLAFEDSPFWIKGDTVEKFGEGAYNEEFIKDVKDGDADPIQLGLNSTWAFYFLEG
                     SKYDWVLSFTHNPGIGGHYAFTGYYPIPKPN"
     gene            1122..1820
                     /locus_tag="PLE93_03960"
     CDS             1122..1820
                     /locus_tag="PLE93_03960"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNH86235.1"
                     /translation="MRLAKYLAHAGVASRRKSEEIVAAGRVRVAGMVITDPAFGVEGG
                     EEVRVDGHQVEPEGREVWMLNKPLDVISTADEPGKRIAVVDLVDSNARLYPVGRLDAD
                     STGLILLSNDGELANRLTHPRFEVEKTYRVKLKKPVTPEAIDQLQRGVRLDDGMTAPA
                     TVKKLGEAELDVTIHEGRNRQVRRMAEAIGNEVVALQRISLGSLKLGRLGIGKCRLLD
                     EREVERLWKDAGDD"
     gene            1813..2199
                     /gene="aroH"
                     /locus_tag="PLE93_03965"
     CDS             1813..2199
                     /gene="aroH"
                     /locus_tag="PLE93_03965"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNH86236.1"
                     /translation="MTDPEAEGMRVAAIRGAVQAENDADGILSATEELIREIISVNDL
                     EPDRMISAIFTTTEDLDAEFPAAAARRMGLNEVPLMCAREIPVPGAMPGVIRVMLHTY
                     LPAGAKARHVYLGETRNLRADLDAAQ"
     gene            2228..>2939
                     /locus_tag="PLE93_03970"
     CDS             2228..>2939
                     /locus_tag="PLE93_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH86237.1"
                     /translation="MTVRFNSHLTSIPGYSPGVPKGHSAEDVASSNLAQLASNESPFA
                     PLPEVVEAITRAAKAMNRYPDPAATRLRQRLADRHEIEPGQVSVANGSCEFLLAAAEA
                     LLEPGAEIVYAWPSFSMYPNMAAMTGAREIRVPLKEDQTHDLEAMLAEITAATQIVIV
                     CNPNNPTGTYIPFAEIADFVERVPDHVQIILDEAYIEFQVYDDPANSIDLLKRHPNLV
                     LLRTFSKTHSLAGLRVAPP"
BASE COUNT          596 a         1003 c          935 g          405 t
ORIGIN      
        1 tggcagcaac aacaacccga ccgtcacgcc ggacacgctc gcctcggcga tggacgaggt
       61 ctgcaccgac accaacgccg acttcgaagc gatgggcacc cgcggcctga ccatggacgg
      121 gatcgcggcc gagttcgagg gctacgccga ggtgcggcag gcagtcgtcg acgggttcaa
      181 cgagttgaac accgacgacc aggccgagcc gcagctccag aagtacatcg acgcctccga
      241 gcaggtcatc gcccaggaca aggcgatcgc caaggccgcc gccgaaggcg accagaaggc
      301 catgaacgag gctttcgccg cccagaccaa ggcattcaac gagcgcgaca aggtggcgaa
      361 ggagatcggc accgaggtct gcggtcagac acccgagatc aaggttgaaa agaccgggac
      421 cgcgccgcct tccgacctca atgtcgtcga gccgaagaac acggtcgagg atgccgccga
      481 caactacatc gaggcgaccg cgtccggcaa ctgcggcctg atcaacgcca accggcacac
      541 cgacgccggt gacctcgatg aagccagctg caaggcagcc ggccaggctc tgaagggcgc
      601 cagggtgatc ggcaccgaga gttacggccc cgtggcccag gccgagatcc tcagcggaaa
      661 cggcacgcac tatccgacct acttcgtcac cgaccttgac ggcaacctcc ggtatggagg
      721 tgacgcgatc cacgacagcg gcggactgcg gccggccccg gaaggaaacg acgccgaggc
      781 gacagtcgag gcgaccgtca acgcgattcg cgacaacgat gccgccgcct tcaacgagac
      841 cctggccttc gaggacagcc cgttctggat caagggcgac actgtggaga agttcggtga
      901 gggtgcctac aacgaggaat tcatcaagga cgtcaaggac ggggacgccg acccgattca
      961 gctcgggctg aactcgacct gggctttcta cttcctggaa ggcagcaagt acgactgggt
     1021 cctttcgttt acccacaacc ccggcatcgg tggccattac gccttcaccg gctactaccc
     1081 gattcccaag ccgaactgat ccggctgaaa tagttccggc catgcggctg gccaagtacc
     1141 tcgcccatgc aggcgtcgcc tcgagaagga aatccgagga gatagtcgcc gctggccggg
     1201 ttcgggtggc cggaatggtg atcaccgacc cggccttcgg ggtcgagggt ggcgaggagg
     1261 tccgggttga cggccatcag gtcgagcccg agggccgtga ggtctggatg ctcaacaagc
     1321 cgctcgacgt gatctccacc gccgacgagc cgggcaagcg gatcgccgtc gtcgacctgg
     1381 tcgacagcaa cgcccggctc tacccggtcg gccggctcga cgccgactcg accggcctga
     1441 tcctgctcag caacgacggc gagctggcca accggctcac ccacccgcgc ttcgaggtcg
     1501 agaagaccta ccgggtgaag ctgaagaagc cggtaacccc ggaagcgatc gatcagctcc
     1561 agcgcggagt ccgcctggat gacggcatga ccgcgcccgc caccgtgaag aagctgggcg
     1621 aggccgaact ggacgtcacg atccacgaag gccgcaaccg gcaagtgaga cggatggccg
     1681 aagcgatcgg caacgaagtg gtcgctctgc agcggatttc gctcgggtcg ctgaagctcg
     1741 ggcgactcgg gatcggcaag tgcaggttgc tcgacgaacg cgaggtggag cggctctgga
     1801 aagatgcagg cgatgactga tccagaggca gaaggcatgc gggtcgcggc gatccgcggc
     1861 gcggtccagg cggagaacga cgccgacggg atcctgtccg cgaccgagga gctgatcagg
     1921 gagatcatct ccgtcaacga cctcgagccg gaccggatga tcagcgcgat cttcaccacc
     1981 accgaggacc tcgacgcgga gttcccggcg gcggcagccc gtcgcatggg tctgaacgag
     2041 gtgccgctta tgtgcgcccg cgaaattccg gtgccgggcg cgatgcccgg ggtgatccgg
     2101 gtcatgctcc acacctacct gccggccggg gcgaaggccc gtcacgtcta cctcggcgag
     2161 acccgcaacc tgcgggccga cctcgacgcg gcccagtagc agcccgccct caacccacgg
     2221 atacacgatg accgttcgtt tcaactccca cctgacttcg atccccggct acagccccgg
     2281 agtcccgaag ggccacagcg ccgaagacgt cgccagctcc aatctggccc agctcgcttc
     2341 caacgagtca ccgttcgccc cgctgcccga agtggtggag gcgatcacca gggcggccaa
     2401 agcgatgaac cggtacccgg acccggccgc gaccaggctg cgccagcgac tggccgaccg
     2461 gcacgagatc gagcccggac aggtatcggt cgccaacggc tcctgcgaat tcctgctcgc
     2521 cgccgccgag gcgctgctcg agcccggggc ggagatcgtg tacgcctggc cctcgttttc
     2581 gatgtatccg aacatggccg cgatgaccgg tgcccgggaa atccgcgttc ccctgaagga
     2641 agaccagacc cacgacctcg aagccatgct ggcggagatc accgccgcga cccagatcgt
     2701 gatcgtctgc aatccgaaca atccgaccgg gacctacatt ccgttcgccg agatcgccga
     2761 cttcgtcgaa cgggtgcccg accacgtcca gatcatcctc gacgaggcct acatcgagtt
     2821 ccaggtctac gacgatccgg ccaactcgat cgacctgctg aagcggcacc ccaacctggt
     2881 cctgctccgc actttcagca agacccactc gctggccggc ctccgggtcg cgccgcccg
//
LOCUS       DAOQFI010000170         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17132_length_2935_cov_3.386111, whole genome shotgun sequence.
ACCESSION   DAOQFI010000170 DAOQFI010000000
VERSION     DAOQFI010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17132_length_2935_cov_3.386111"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLE93_03975"
     CDS             complement(<1..310)
                     /locus_tag="PLE93_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86238.1"
                     /translation="MLAVVLISLGLGLAVSACGSSSDEGDSGGSGKEGGEITISQTSQ
                     PDYLDPALSYTVNGWEPMWIVYTPLLTYPHVEGTKGGEVIPGLAEDLPKVSNGGKTYE
                     L"
     gene            complement(467..1039)
                     /locus_tag="PLE93_03980"
     CDS             complement(467..1039)
                     /locus_tag="PLE93_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH86239.1"
                     /translation="MSSSSRPEILRATRTLIAKNGAQVSLAEVASEAGVSRQAVYMHF
                     GSRAGLLVALVRNMDEEAGIRQKLERALLREDPVEAFRQFVKGWLRYAAEIQPVASML
                     FAAREIDPAAAKAWEDRSRELRAGFRAATVRLGKAGMLRDGLSATSAASLSWAICSVP
                     VVEQLALDQEWSKDRVARQLTEAALSAITG"
     gene            1072..1479
                     /locus_tag="PLE93_03985"
     CDS             1072..1479
                     /locus_tag="PLE93_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH86240.1"
                     /translation="MSEHPALSAAREAMAAVSAGDRTAWLDLYDDDAVLEDPVGGSPL
                     DPEGVGLRGRTALEGFWDLVVGPNDFDFEITATHSGGSEAALSATVRGRYPNGAKTSY
                     DGIFIYTIGENGKITSVRAFWDIDAVMSALGAS"
     gene            1557..1931
                     /locus_tag="PLE93_03990"
     CDS             1557..1931
                     /locus_tag="PLE93_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HNH86241.1"
                     /translation="MGRDSELVWSSDGGDFRPEQKREAKKAKRRGGISGAAPPPGQAP
                     AGSTIKVKRETSGRKGKTATTISEIPLGEAETKALAKKLKQHCGVGGTARGSSIELQG
                     DHRDKVIAYLEKEGHRAVKAGG"
     gene            complement(1928..2881)
                     /locus_tag="PLE93_03995"
     CDS             complement(1928..2881)
                     /locus_tag="PLE93_03995"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNH86242.1"
                     /translation="MGTLLDVDHGTYPFVTSSSAAAGGACTGTAACVVTLDQARQVTA
                     SFALEKRSLGVTRSGNGSGTVMSQPAGVACGGQCEGIEFDYGTEVTLSANPATSSDFS
                     GWTGDCSGSGSTCIVTLDQARSVEATFTLQKRTLTVETSGTGSGTVGTDPAGPDFDFG
                     TTVTLEAVPANGSTFTGWGGDCSGIVPTCTVDMSDARSVEARFTADPLPDPAPVIGQL
                     RVAPRKVPLKGKKKSAIRRQGPAIRIRLSEASTVRFSLKRKPGKARNFRRKLKAGTSK
                     VRIPAGVRKLLRPGRFQLTAVATDNAGQSSNPRRTGFRVIR"
BASE COUNT          518 a          988 c          933 g          496 t
ORIGIN      
        1 tgagctcgta agtcttgccg ccgttgctga ccttgggcag gtcctcggcg agtccgggaa
       61 tgacctcgcc gcccttggtg ccttcgacgt gcgggtaagt gagaagcggg gtgtagacga
      121 tccacatcgg ttcccagccg ttgaccgtgt aggaaagcgc cgggtccagg tagtcgggct
      181 gtgaggtctg ggagatcgtg atctctccac cctccttgcc gcttcctccc gagtcgcctt
      241 cgtccgagct ggacccacag gcggacacag ccaacccgag ccccagactg atcagaacga
      301 cagcgagcaa cttcccaatc aacgaatccc gcaaatcacg aaccaggttc atggacgaaa
      361 cgcctctcct cgttgtacag accttgagca cattggctcc tctctccccc gtcaggctag
      421 cgccaaaacc ggcgcttcgg gagaaatccg tctcacccgc ctcgattcaa ccggtgatcg
      481 cgctcagcgc ggcttcggtc aactgccttg cgacccggtc ctttgaccat tcctgatcga
      541 gtgccagctg ctcgaccacc ggcaccgaac aaatcgccca ggacaggctc gcggccgaag
      601 tcgccgaaag cccgtcccgc agcattcccg ccttccccag tcgcaccgtc gccgcacgga
      661 accccgcgcg aagttcccgg gaacggtcct cccaggcctt tgccgcggcc gggtcgatct
      721 ccctcgcggc gaaaagcatc gaggcgaccg gctggatctc cgccgcgtac ctcagccacc
      781 ccttcacaaa ctggcggaat gcctcgaccg ggtcctcccg cagcagcgcc cgttccagct
      841 tttgcctgat tccggcctcc tcgtccatgt tgcgaaccag ggcaacgagc agacccgcac
      901 gggacccgaa gtgcatgtac accgcctggc gggaaactcc ggcttcactg gcaacctcgg
      961 cgagcgagac ctgggcgcca ttcttcgcga tcagggtcct ggtggccctg aggatctccg
     1021 gtcgggacga acttgacatg tgacaagttt acggtaactt tacacgtgta aatgtctgaa
     1081 cacccagcac tttccgctgc ccgcgaggcc atggccgccg tttccgccgg agaccgcact
     1141 gcctggctcg acctctacga cgatgatgcg gttctcgaag atccggtcgg cgggtctccg
     1201 ctggacccgg agggggttgg cctgcgaggc cgaaccgccc tcgaagggtt ctgggacctc
     1261 gtggtcggtc cgaacgactt cgatttcgag atcaccgcga cgcattccgg aggctccgag
     1321 gcagcactct cggccaccgt ccgcggcagg taccccaacg gcgcaaagac cagctacgac
     1381 ggcatcttca tctacacgat cggtgaaaac ggaaagatca cttcggtccg cgccttctgg
     1441 gacatcgacg ccgtgatgag cgcgctcggc gcttcctgat tgccggccgt aggcgcgagg
     1501 tcaactgaag caacaccgag gtcgtccgga agcagaccgg cgggtagggt tccgccatgg
     1561 gtcgtgactc ggaactcgtc tggtcaagcg acggcgggga tttccgcccg gaacagaagc
     1621 gggaagccaa aaaggcgaaa aggcgcggag gcatatccgg cgcggcaccc ccgcccggcc
     1681 aggcgccggc cggctcgacg atcaaggtga agcgggaaac ctcgggccgg aaggggaaga
     1741 ccgccacgac gatctcggag atccccctgg gcgaagccga gaccaaagcc ctggcgaaga
     1801 agctgaagca gcattgcggg gtcggcggga ccgcgagggg ttccagcatc gaactccagg
     1861 gcgaccaccg cgacaaggtg atcgcctacc tcgaaaagga aggccaccgg gccgtaaagg
     1921 ccgggggcta gcggatgacc ctgaatccgg ttcggcgtgg gttcgagctc tgaccggcgt
     1981 tgtcggtcgc tacggcggtg agctggaacc ttcccggcct gaggagcttg cggaccccgg
     2041 ccgggatccg gaccttgctg gtgccggcct tgagcttgcg cctgaagttc ctggccttgc
     2101 ccggcttgcg tttgaggctg aaacgaacgg tcgaggcttc gcttagcctg atcctgatcg
     2161 ccggtccctg gcgccggatc gccgacttct ttttgccctt gagcgggacc tttcgcggcg
     2221 cgaccctgag ctgaccgatc accggtgcgg ggtccggcaa cgggtcggcg gtgaacctgg
     2281 cctcgactga ccgggcgtct gacatatcga ccgtgcaggt cggcacgatt ccggaacagt
     2341 ccccgcccca cccggtgaac gtcgagccgt tcgcgggaac cgcttccagg gtgaccgtgg
     2401 ttccgaagtc gaaatcgggg ccggccgggt ctgtaccgac tgtgcccgac ccggtgcctg
     2461 aggtctcaac cgtcagcgtc cgcttctgaa gggtgaacgt ggcctcgacg gaacgggcct
     2521 ggtcgagggt gacgatgcag gtggaacccg atcccgagca gtccccggtc catccgctga
     2581 aatcggaact cgtggccggg ttcgccgaga gggtcacttc ggtgccgtaa tcgaactcga
     2641 tgccttcgca ttggccacca caggcgactc ccgccggctg cgacatgacc gtgcccgaac
     2701 cgttgccgga ccgggtcacg cccaggctgc gcttctcgag cgcgaaggag gccgtgacct
     2761 gacgggcctg atccagggtg accacgcaag ccgcggttcc ggtgcaggcc ccgccggccg
     2821 ccgcgctgga actggttacg aagggatagg tcccgtggtc cacgtccagg agggtgccca
     2881 cgtcgccggc ctccatgccc tcggccgcga tgtcctcggc gtcgtagtcg tctca
//
LOCUS       DAOQFI010000171         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17194_length_2929_cov_3.373347, whole genome shotgun sequence.
ACCESSION   DAOQFI010000171 DAOQFI010000000
VERSION     DAOQFI010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17194_length_2929_cov_3.373347"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLE93_04000"
     CDS             complement(<1..253)
                     /locus_tag="PLE93_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86243.1"
                     /translation="MNRFAGTALLVLTILLGAVTTASAARDFWNPASSDSSQQFPLLP
                     PPLDGNVQRDDTPNDPGYDRAEPDDEDGATTTNIYDERFD"
     gene            complement(392..2227)
                     /locus_tag="PLE93_04005"
     CDS             complement(392..2227)
                     /locus_tag="PLE93_04005"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNH86244.1"
                     /translation="MRVRRLAVMGLLAALAGCLVAAPAQAAPQPKMTAVKAPPKLAVG
                     GRYKVTSIVTNRSGSRVRGPITLTLSKDRKPGAVKLGSRRATASGKGRSRIVTRATLK
                     KSIAVGRYFLVSCFGFPGGSTCAARRVTVIKYVNGARSGGDSLFPEIGNGGYDALHYE
                     ISLDYDPLTNQFNPGTYTKMTARATEYLRQFSLDFEGPEISRIEVNRRRSNFIRRGTK
                     LIITPRIRLDAGRRFTVKVEYSGYVNHHVDPDESLEGWVRACRSGDPGPDNPDCFGSF
                     TVSEPIGAQTWFPNNNVPSDKATFTTTTAVPTEAGADEWTALGTGEFVSKQEGTDGKT
                     TWKWRESLPTSTYLTTGSVCRCVYDVASATDSSNNHTFPLYNAYDNSATPTQVDAINT
                     QLGLQEDIINEFSALFGPYPLKSGGVFAGRTSGIGYVLENQGKIHFPFPGIGNLTLAH
                     EYVHQWFGDAVGPASWSQIWFNEGWAQWGEWFHESPTTPADAFTTEYESTENPERWDI
                     PPGTLNDDPSLLFDEFSTYIRPGMMLEGYRQIVGDAKFSALAKAIQTDYAYSTIDADQ
                     FIATALEVADFGPVQEAALNDYFQQWLFLAGKPSLTPGNFPPEPL"
     gene            complement(2258..>2929)
                     /locus_tag="PLE93_04010"
     CDS             complement(2258..>2929)
                     /locus_tag="PLE93_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86245.1"
                     /translation="SAYVVATVIRNPAANIARGSIRITLSTNRGSGPWLLGRRKVAGV
                     APRGRKSVLAGARIKRSTGTGKYFVVSCFRGGWGSTCGATSVRVVPIATGPPGKDGTT
                     GATGSTGATGGTGPIGPIGPTGNTGSTGPTGPTGVTGFTGPTGATGATGATGVTGVTG
                     PIGPTGATGLTGTTGSTGLTGPTGATGLTGSTGLTGPTGSTGLTGSTGLTGSTGFTGP
                     TGPVG"
BASE COUNT          448 a          931 c         1003 g          547 t
ORIGIN      
        1 gatcgaagcg ctcgtcgtag atgttcgtgg tcgtcgcgcc gtcctcatcg tccggctcgg
       61 cgcggtcgta gcccgggtca ttgggggtgt catcgcgctg cacgttgccg tcgagcggcg
      121 gcggcagaag cgggaactgc tgcgagctgt cactcgatgc ggggttccag aagtcacggg
      181 cggccgaggc agtggttacc gctcccagca ggatggtcag gaccagcagg gctgtgccgg
      241 cgaatctgtt cactcccaag ctcccggttt ggtagacgag tactggaacc tttatgtgca
      301 gatccgccgg ttctcaaaca ggccggagcc cttccttccg taaacccggt gcggaaggag
      361 gcgggtctgg ccggcgaccc gcgaacggcg cctaaagagg ttccggcggg aagttgcccg
      421 gagtcagtga aggtttgccc gcgagaaaca gccactgctg gaagtagtcg ttcagggccg
      481 cttcctgtac cggcccgaag tcggcgacct cgagggcagt ggcgatgaac tggtccgcgt
      541 cgatcgtcga gtatgcgtag tccgtctgaa tggctttggc gagcgcggag aacttcgcat
      601 caccgacgat ctggcgatag ccctcgagca tcattcccgg ccggatgtag gtcgagaatt
      661 cgtcgaacag cagggacgga tcgtcattca gggttccggg cgggatgtcc caccgctccg
      721 gattctcggt cgattcgtac tcggttgtga aggcgtcggc cggtgtggtc ggtgactcgt
      781 ggaaccactc gccccactgg gcccagccct cgttgaacca gatctggctc cagctggcgg
      841 ggccgaccgc atcgccgaac cactggtgga cgtattcgtg ggccagggtc aggttgccga
      901 tcccggggaa cgggaagtga atcttgccct ggttttccag cacgtagccg attccggaag
      961 tccggccggc gaagactcct ccggacttca gcgggtaggg cccgaacagg gcggagaact
     1021 cgttgatgat gtcttcctgc aatccgagct gggtgttgat cgcatcgacc tgggtcggcg
     1081 tggccgagtt gtcgtaggcg ttgtagagcg ggaaggtgtg gttattcgag ctgtcggtcg
     1141 ccgaggcgac gtcatagacg cagcgacaga cggagccggt ggtcaggtag gtcgaggtcg
     1201 gcagggattc tcgccacttc caggtcgtct tgccgtcggt tccttcctgc ttcgagacga
     1261 attcccccgt ccccagcgcg gtccattcgt cggcgccggc ttccgtcggt accgcggtcg
     1321 tggtggtgaa ggtcgccttg tcagacggga cgttgttgtt cgggaaccag gtctgggcgc
     1381 cgatcggctc gctcaccgtg aaggagccga aacagtccgg gttgtcggga cccggatccc
     1441 ccgagcgaca ggcgcgaacc cagccttcga gtgattcgtc ggggtcgacg tgatggttga
     1501 cgtaacccga gtactcgacc ttgaccgtga acctgcggcc ggcgtccagg cggatccgcg
     1561 gggtgatgat cagtttcgtg ccccgccgga tgaagttgga gcgccttcgg ttgacttcga
     1621 tccgggagat ctcggggccc tcgaagtcga ggctgaactg ccgcaggtat tccgtggccc
     1681 gggcggtcat cttcgtgtag gtgcccgggt tgaactggtt ggtcaagggg tcatagtcga
     1741 ggctgatctc gtagtggagc gcgtcatagc cgccgttgcc gatctcggga aagagcgaat
     1801 ccccgccgct gcgggcgcca tttacgtact tgatgacggt gacccgcctg gcggcgcagg
     1861 tggatccccc cgggaacccg aaacaggaga cgaggaagta cctgccgacg gcgatcgact
     1921 tcttcagggt ggcgcgcgtg acgatccggc tcctgccttt cccgctcgcg gtcgccctgc
     1981 gggatccaag cttgacggcg cccggcttcc ggtctttcga aagggtcaga gtgatcggtc
     2041 cccggaccct ggatccggaa cggttggtca cgatcgaggt caccttgtac cggccgccca
     2101 ccgccagctt cggcggtgcc tttaccgccg tcattttcgg ctgcggtgcg gcctgggccg
     2161 gcgcggccac aaggcagcca gccagtgcgg ccagtaaccc catgactgcc agcctgcgga
     2221 ccctcatcct tctgccttcc gggtccggcc cggacatcta gccgaccggc cctgtgggcc
     2281 cggtaaaccc ggtcgagccc gtcaaaccgg tcgagcctgt caaaccggtc gagccggtcg
     2341 gtccggtcag accggtcgag cccgtcagcc cggttgcgcc ggtcggtccc gtcagacccg
     2401 tcgagccggt cgtaccggtc aacccggtgg cgccggtcgg tccgatcggt ccggtgacac
     2461 ccgtgacgcc tgtcgccccg gtggcccccg tcgccccggt cggtccggtg aagcccgtca
     2521 ccccggttgg ccctgtggga ccagtcgagc cggtgttccc ggtcggcccg atcggtccga
     2581 tcggcccggt gccgcccgtc gccccggtac ttccggtggc gccggtcgtc ccgtccttgc
     2641 ccggcgggcc ggtggcgatc ggaacgaccc ggacggatgt cgcgccacag gtgctacccc
     2701 atccaccacg gaaacaggaa acgacgaagt acttcccggt gccggtcgag cgcttgatcc
     2761 gggccccggc cagaacggat tttcttcctc tcggggcgac cccggccacc ttgcggcggc
     2821 caagcagcca cgggcccgaa ccgcgattcg tcgagagcgt gatcctgatc gagccccggg
     2881 caatgttcgc ggccgggttg cgaatcaccg tggccacgac ataggcact
//
LOCUS       DAOQFI010000172         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17231_length_2925_cov_3.357840, whole genome shotgun sequence.
ACCESSION   DAOQFI010000172 DAOQFI010000000
VERSION     DAOQFI010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17231_length_2925_cov_3.357840"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2760
                     /locus_tag="PLE93_04015"
     CDS             <1..2760
                     /locus_tag="PLE93_04015"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH86246.1"
                     /translation="ASPDLRDGVGSIIAVNGVPATPITVDISGVTVSGGGTEAFLPSS
                     SIWNGEFLNGVYAEAGVSFLDAGGSLNASRVTNIVTSNDPANAEPGGYRSNNVGWGVT
                     QVTAAGGSGTATLPISLIGTRIDRYNKGGLLVSGSTGDTPPLTRAGTIGQATVTASSI
                     IGRNLNYPANDGTAPGTLVSTGAVFGQDGIQVTSGSTLTATSNLISQNLMAGTGTATA
                     AAIRGAAGIRLIDIGTNTVATKSNILYNGYGIINLDADGVTDNTTTTIDAPLNYWGMS
                     DASGTVNTGPAVSPAAVPAYPGNPVNGAADVTYGSNSVHFLPFRSGPKTEPGTIANPE
                     LGGQWPQADAPIPVADSAPTSALSSNTDTVAPGGTLTLTAHANDDFGVKSVTFYKGAT
                     PLGTVVPPNDSITWDAPATCGGSNQSFSVTVVDSNDQSSSSSLSVAIDDCDPAVSLSA
                     APTAPLGTIRLSADASDDLGLASLDFYVDGDLIHTVSNPDNGISTYDYVPETLCGADE
                     FGFEVVATDSTGHQTADSVNYSKDDCDPVVTLAANHASVAPGGAVDLIAEAEDDVEVA
                     QLTFFKAASQLASYPNTDHTAAWVKVHSTTASNLCGTTDHFRVVAEDNAGQTSEDTVD
                     VSTTACPDNPPTIDLGASATTVDPGDDVTLTATASDDHGVSSITFFEGATQIGQITPP
                     ANSIVWTAPETCDTSYTLKAVARDTANQTTEDTVMVTVNACPPPAEPTVSIDSPPAQI
                     AQNGTTVSATATSGADVSKVTFFLGIRQVCEDTSAPFSCKLVPNGDEVGAQPIKAVVT
                     DSLGRTAEDNESTTVSKFKPKGLSLSAKRIGSKKRRIKASGKLMLPARMTAADGCSAS
                     RVAITAKLGKKSLVNKQVKLASNCSYSLAFGAPKTKKKQRVVIKARFAGNDSLSATSQ
                     VRKVR"
BASE COUNT          576 a         1043 c          869 g          437 t
ORIGIN      
        1 gcctctcccg acctgcggga cggagtcggc agcatcatcg cggtcaacgg cgtcccggcc
       61 actccgatca cggtcgacat ctccggcgtg acggtctccg gcggtggtac cgaggccttc
      121 cttcccagct cgtccatctg gaacggcgag ttcctgaacg gtgtctacgc cgaagccggc
      181 gtctccttcc tcgacgcagg tggttcgctg aacgccagcc gcgtcaccaa catcgtcact
      241 tccaacgacc cggccaacgc cgagcccggt ggctaccgca gcaacaacgt cggctggggc
      301 gtcacccagg tgaccgccgc cggtggcagc ggaactgcga ccctgccgat ctcgctaatc
      361 ggtacccgca tcgaccgcta caacaagggc ggcctgctgg tcagcggttc aaccggcgac
      421 actccgcccc tgacccgggc cggcacgatc ggtcaggcca cggtgaccgc ttcgtcgatc
      481 atcggacgca acctgaacta cccggccaat gacggaaccg cgccggggac cctggtctcg
      541 accggtgcgg tgttcggtca ggacggcatc caggtcacct ccggttcgac gcttaccgcg
      601 accagcaacc tgatttcgca gaacctcatg gcgggcaccg gtacggcgac cgccgccgcg
      661 atcaggggtg ccgccggcat ccgactgatc gacatcggaa ccaacacagt ggccacgaag
      721 agcaacatcc tctacaacgg ctacggaatc atcaatctcg acgccgacgg cgttaccgac
      781 aacacgacga cgacgatcga tgcacccctc aactactggg gcatgtccga cgcgtccggc
      841 acggtcaaca ccggccctgc cgtgtcgccc gccgcggtcc cggcctaccc gggtaacccg
      901 gtcaacggcg ccgctgacgt gacctacggt tcaaactcgg tccacttcct gccgttccgc
      961 agcggcccca agacggagcc cggaaccatt gccaatcccg agctcggtgg ccagtggccg
     1021 caggccgacg caccgattcc tgtcgcagac agcgccccga cttcggccct ctcttcgaat
     1081 acggacacgg tggccccggg tggcaccctc accctgaccg cgcacgcaaa cgatgacttc
     1141 ggcgtcaaga gcgtgacctt ctacaagggc gcgaccccgc tcggcacggt tgtcccgccg
     1201 aacgactcga tcacctggga cgctcccgcg acctgtggcg gcagcaatca gtcgttctcg
     1261 gtcaccgtcg ttgactcgaa cgaccagtcg tcctcctcga gcctctcggt ggcgatcgac
     1321 gactgcgatc ccgccgtctc cctgagcgcg gcgccgaccg cgccgcttgg cacgatcagg
     1381 ctcagcgccg acgcaagcga cgatctcggt ctcgcttcgc ttgatttcta cgtggacggc
     1441 gacctgatcc acacggtcag caacccggac aacggaatct cgacctacga ctacgtgccg
     1501 gagaccctct gtggcgcgga cgagttcggg ttcgaagtag tcgcgaccga ctccaccggt
     1561 caccagactg cggactcggt caactacagc aaggacgact gtgatcccgt ggtcaccctc
     1621 gcggccaacc acgccagcgt cgctcccggc ggagcggttg atctgatagc cgaggccgaa
     1681 gacgatgtcg aggtcgcgca gctgaccttc ttcaaggctg cctcgcagct ggccagctac
     1741 cccaacacgg atcacacggc ggcctgggtc aaggtccact cgaccaccgc atccaacctc
     1801 tgcggcacga ccgatcactt ccgggtggtc gccgaggaca acgccggcca gacctcggag
     1861 gacacggttg acgtttccac cacggcctgc cccgacaacc cgcctacgat tgacctcggc
     1921 gcaagcgcga ccacggtcga tccgggtgac gacgtcaccc tgaccgcgac cgccagcgac
     1981 gatcacgggg tcagcagcat caccttcttc gaaggtgcga cccagatcgg ccagatcact
     2041 ccgccggcca actccatcgt ctggacagcc cccgaaacgt gtgacacgag ttacacgctg
     2101 aaggcggtcg ccagggacac cgcgaaccag accacggagg acacggtgat ggtgaccgtg
     2161 aacgcctgcc cgccgccggc cgagccgacg gtgagcatcg actcgccgcc tgcgcagatc
     2221 gcgcagaacg gcacgaccgt cagcgccacc gcgaccagcg gggcggacgt ttccaaggtg
     2281 accttcttcc tcggcatccg ccaggtttgc gaggacacgt cggcaccgtt cagctgcaag
     2341 ctcgtcccga acggcgacga agtaggagcc cagccgatca aggcggtcgt caccgacagc
     2401 ctcggtcgca cggccgagga caacgagtca accaccgtgt cgaagttcaa gccgaagggc
     2461 ctgagcctca gcgcgaagcg gatcggcagc aagaagcggc ggatcaaggc ttccggcaag
     2521 ctgatgctgc cggcccggat gaccgcggcc gacggatgca gcgcctcgcg ggtcgcaatc
     2581 accgcgaagc tgggcaagaa gtcgcttgtc aacaagcagg tcaagctcgc gtcgaactgc
     2641 agctactcgc tggcgttcgg agctccgaag acaaagaaga agcaaagggt ggtgatcaaa
     2701 gccagattcg ccggcaatga ctcgctctct gccaccagcc aagtgagaaa ggtccggtga
     2761 tcaggttgaa gacgatcgaa cacgattcca acggactgat cacagggagc aagaacatga
     2821 tctccatgcg caagaaaatc actggtgtcc tcgcggccct ggttgcggcg attgccctca
     2881 cgggtgtctt cgccggccag gcccaggcaa tcacctacga cgcgt
//
LOCUS       DAOQFI010000173         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17398_length_2910_cov_2.792995, whole genome shotgun sequence.
ACCESSION   DAOQFI010000173 DAOQFI010000000
VERSION     DAOQFI010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17398_length_2910_cov_2.792995"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLE93_04020"
     CDS             complement(<1..724)
                     /locus_tag="PLE93_04020"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HNH86247.1"
                     /translation="MRPQSNSRFALFILPALVAALFAVLSAGAQAASPLSIEVLSSKA
                     DRISGGDALVAVTAPDSSAKSKMVVRLNGKVVTSSLASDPDNSLRKKGLVSGMWNGRN
                     WITAWAPGQDSATRLSVFNSSLQGPVFSGPHQTPYYCTTESFGLGPAQDSNCVAPTRV
                     SWYYRASDNTFKPLTSPADRPADMVQTTTRTGQLVDYVVRLEQGVINRAVYNFATLAS
                     GGQTGNGWNGRFVYQYGGGCSTG"
     gene            854..1612
                     /locus_tag="PLE93_04025"
     CDS             854..1612
                     /locus_tag="PLE93_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNH86248.1"
                     /translation="MPIPEKIQGFIDRQADEAPVKYDDLRAVVFNGTLKRSPEPSQTE
                     ALLAIPIGILERVGVRVDLVRTVDHVIPPGVWPDMTGHGYEEDDFPAIYRELVEPADI
                     IILAGPIWLGDQASPTRAIIERLYAYSGEVNDAGQWSYYGKVGGAVTTGNEDGGKHVS
                     AQLLYALQHIGLTIPPQTDSYWNGEAGPGDSYADPGSGGPENAWTTRNATFMTWNILH
                     FARMLKDAGGVPAYGNSTRDWDLTQPDHPNPEYR"
     gene            1673..1745
                     /locus_tag="PLE93_04030"
     tRNA            1673..1745
                     /locus_tag="PLE93_04030"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1706..1708,aa:Val,seq:gac)
     gene            complement(1797..>2910)
                     /locus_tag="PLE93_04035"
     CDS             complement(1797..>2910)
                     /locus_tag="PLE93_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86249.1"
                     /translation="NVADWNNGTDGSPKGVAGTYLCHQNCSWGASDLTAELGKVTDFL
                     ASHPREVLIFMVEDGIYPQDFATAVNDSALSDYIYTGPTDSYPTLGEMIASNQRVVVF
                     SENHTGNDSDYAEVPWYHRGYNGPMQETPYNFRKDESNVDLTTQQAMAQLTDSANLNS
                     SCRPNRGGTTGQLFLMNHWVNGLIDAGTTGNTFTPDPAVAQVLNQPDVLVARAQACEA
                     RRGKLPNIVAVDDFGDGDLTDAVRQLNGVTAKPFFEVAKPKGVTVKSGARASFRLQIR
                     NWGSAESALTRVCASVPGKLAIKPKCQTVFVTQANPGKGTATVKVSTRRIYQGRFKGS
                     KSGPVRFTITGGGDSVTTSSTLKVNPLKPKKPKRRN"
BASE COUNT          488 a          920 c          966 g          536 t
ORIGIN      
        1 ggccggtcga gcaaccgccg ccgtactgat agacgaaacg gccgttccag ccgttacccg
       61 tctggccgcc ggaggccagc gtcgcgaagt tgtagaccgc acggttgatc acaccctgct
      121 cgagccggac cacgtaatcg accagttgac ccgtgcgggt ggtggtctgg accatgtccg
      181 cgggccggtc ggccgggctg gtcagcggct tgaaggtgtt gtcgctggcg cggtagtacc
      241 aggagaccct ggtcggggcg acgcagttcg aatcctgcgc cgggccgagt ccgaaactct
      301 cggtcgtgca gtagtacggg gtctggtggg gcccggagaa gaccggcccc tgaagcgagc
      361 tgttgaagac cgaaaggcgg gtcgcagaat cctggcccgg agcccaggcg gtgatccagt
      421 tgcggccgtt ccacatcccg gagacgaggc ccttcttgcg gagcgagttg tcgggatcgc
      481 tggcgaggga ggaagtcacg accttcccgt tcagccggac gaccattttg ctcttcgcgc
      541 tcgagtccgg agccgtgacg gcgaccagcg cgtcaccgcc ggaaatccgg tcggccttgc
      601 tcgagagcac ctcgatcgaa agcggactcg ccgcctgcgc ccccgcggag agcacggcga
      661 aaagtgccgc gacgagtgcc ggaagaatga aaagcgcgaa cctggagttc gactggggtc
      721 tcaagactgc tcctggaggg gtggggggag ggtgaccgct actgaccttc tgtatctaga
      781 taacgcgccg cgttcgctag ttgcggtcgg cgtcggcttg acggccatca cccctgcgga
      841 gaggatgttc cccatgccaa ttccggagaa gattcagggt ttcattgaca ggcaggccga
      901 cgaggcgccc gtgaagtacg acgatctgag agcggtggtt ttcaatggca ctctcaagcg
      961 atctccggag ccgagccaga ccgaggcgct gctcgctatt ccgatcggga tcctcgaacg
     1021 ggtcggcgtc agggtcgacc tcgtccgcac cgtggatcac gtgatcccgc ccggagtctg
     1081 gccggacatg accgggcacg gttacgaaga agatgacttt ccggcgatct accgtgaact
     1141 ggtcgagccg gctgacatca tcatcctcgc cggtccgatc tggctgggtg accaggcctc
     1201 cccgacccgg gcgatcatcg aacggctcta cgcctattcc ggcgaggtaa acgatgccgg
     1261 tcagtggtcc tattacggca aggtcggggg agcggtcacc accggcaacg aggacggcgg
     1321 caaacacgtg agcgcccagc tgctctacgc gctgcagcac atcgggctga cgatcccgcc
     1381 gcagaccgac tcgtactgga acggtgaagc cggccccggt gattcctacg ccgatcccgg
     1441 gagcggtggc ccggagaacg cctggaccac ccgcaacgcc accttcatga cctggaacat
     1501 cctccatttc gcgcgcatgc tgaaggacgc cggtggcgtg cccgcctacg gcaactcgac
     1561 cagggactgg gacctcacgc agcccgacca tcccaacccg gaataccgat gaggggagtt
     1621 gcccgtgcgg cggtgcggac cggggcggcc ctgcgctagg ctctccgtcc ccgggcgcat
     1681 agctcagttg gtagagcgcc acctcgacac ggtggaggtc actggttcga gcccagttgt
     1741 gcccactcgg aaacccggcc ctccagccgg gtttcgtgtt tcagcgggag cccggctcag
     1801 ttccggcgct tcggcttctt cggcttgagc gggttcacct tgagggtgga cgaggtcgtg
     1861 acgctgtcac caccgccggt gatcgtgaac ctgaccgggc cggacttgct gcccttgaac
     1921 cggccctggt agatcctgcg ggtgctgacc ttcacggttg ccgttccctt gccggggttg
     1981 gcctgggtca cgaagaccgt ctggcatttc ggcttgatcg ccagcttgcc ggggacgctc
     2041 gcgcagaccc tggtcaacgc cgattccgcg ctgccccagt tcctgatctg gagcctgaag
     2101 ctcgccctgg cgccggactt gacggtgacg ccctttggct ttgcgacctc gaagaacggc
     2161 ttcgcggtca cgccgttcag ctgccggacc gcgtcggtca ggtcaccatc accgaagtca
     2221 tcaacggcaa cgatgttcgg cagcttgccc cgacgcgcct cacatgcctg cgcccgggcg
     2281 acgagcacat cgggctggtt gaggacctgt gcgaccgcgg ggtcgggagt gaaggtattg
     2341 ccggtcgtgc cggcgtcgat cagtccgttg acccagtggt tcatcaggaa gagctgcccg
     2401 gtggtgccgc cccggttcgg ccggcaactc gaattcaggt tggccgaatc ggtcagctgc
     2461 gccatcgcct gctgagtagt caggtcgacg ttggattcgt ccttgcgaaa gttgtacggg
     2521 gtttcctgca tcggtccgtt gtaaccgcgg tggtaccagg gaacctccgc atagtcggaa
     2581 tcattgcccg tgtggttttc cgaaaacacg accaccctct ggttgctcgc gatcatctcc
     2641 cccagcgtgg ggtacgagtc ggttggaccg gtgtagatgt agtcactcag ggcgctgtca
     2701 ttcaccgcgg tggcgaagtc ctgggggtag atcccgtcct cgaccatgaa gatcagtact
     2761 tcgcggggat gcgaagccag aaagtcggtg accttgccga gctcggcagt caggtcgctc
     2821 gctccccagc tgcagttctg gtggcagagg taggtgccgg cgacgccctt cgggctaccg
     2881 tcggtcccgt tgttccaatc cgcgacattc
//
LOCUS       DAOQFI010000174         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17577_length_2892_cov_3.152274, whole genome shotgun sequence.
ACCESSION   DAOQFI010000174 DAOQFI010000000
VERSION     DAOQFI010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17577_length_2892_cov_3.152274"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLE93_04040"
     CDS             <1..534
                     /locus_tag="PLE93_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH86250.1"
                     /translation="LASLLLRLTEPGSGRISCGGTDLSEVDPQSWRRQIAWVPQRPTV
                     FADTLAANLRLFNPQATDAQLAEAIGVAVLGEVVRGLPDGLETMVGEGGRRLSAGQTQ
                     RIALARALLSEAPLLILDEPTAHLDQDTEKELAEAINEAASGRTALIISHREQPIARA
                     DRTLLLNQGRLGEMVNA"
     gene            531..2210
                     /gene="cydC"
                     /locus_tag="PLE93_04045"
     CDS             531..2210
                     /gene="cydC"
                     /locus_tag="PLE93_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HNH86251.1"
                     /translation="MIAARLERTPLGRAALATRNRWKLFTISVLLGTGTVLAAVGLLT
                     ASGYLISRAAERPEILSLTVAIVGVRFFGVSRALLRYGERLTSHDLAFRTLTDLRGRF
                     FERLIPLVPAGLDGSRRADLMSRFVGDVDRLQDLYLRALSPPLVAIFCSVFCVALASI
                     ILPVAGLVLAVMLLLGGLAAPALTRWAARTAGRRQSRARGLLAMDLHEIAVGGAEIAV
                     AGRESDWLERSREDDRHLVSLQRRDAMSGGLAEGLGTLLAVGAAVAVTAASIPAVHSG
                     ELNGVLLAALALLAMASFEAITPLSQAAAVIDATDEAAERIEEVSERPVPILNPEHPR
                     PVPAGGPIRLDGVSFAYPGGDGLLLRDADLEIRPGEAVALIGPSGIGKSTLSELLVRF
                     RDPDRGRITLGGADIRDLDQEELREAVRLAPQDAYMFNTTIAENVKLGRPEADRERIV
                     EALAEVGLEDWINSLPDGIDSLVGEDGSMISGGQRQRIAAARVFLSRARFLIFDEPTA
                     HLDPDGAAALESHLVDRRDQGCGILVITHTIADPQAFDRVLELRDGTFREV"
     gene            complement(2289..>2892)
                     /locus_tag="PLE93_04050"
     CDS             complement(2289..>2892)
                     /locus_tag="PLE93_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH86252.1"
                     /translation="RGRWARELHDETLQNLAGLRVLLSGTRRRTAMGPVSPILDQAIE
                     RIDETIVELRRLIADLRPAALDELGVEAALSSLVERLSSNGVEIDLYVELRPEDEMGG
                     RYEPLLEDTVYRLVQESLNNAIRHGHAVRAIVEVIEEEGEIRIRVIDDGSGFEPNRAG
                     GGGFGLLGMRERVNLTGGHFDVRSGEDGTTITASLPARQP"
BASE COUNT          422 a          990 c          992 g          488 t
ORIGIN      
        1 ctggcttcgc tccttctgcg gctgactgaa ccgggttccg gccggatcag ctgtggcggc
       61 actgacctgt ctgaagtcga tccgcagagc tggcggcggc agatcgcctg ggtgccccaa
      121 aggccgaccg tcttcgccga cacactggcg gcgaacctga ggttgttcaa tccgcaggcc
      181 accgacgccc agctggccga agcaatcggg gtggcggtgc tgggtgaagt ggtcaggggg
      241 ctgcccgacg gcctggaaac catggtcggc gaaggaggcc ggcggctgtc ggctggtcaa
      301 acccagagaa tcgccctcgc cagggccctt ttgagcgagg cccctctgct gatcctcgat
      361 gaaccgaccg ctcacctcga ccaggacacc gagaaggaac tcgcggaagc gataaatgaa
      421 gccgcgagtg gccggaccgc cctgatcatc tcccatcgcg agcagccgat cgcgagggcc
      481 gaccggaccc tgctgctgaa ccaggggcga ctgggtgaaa tggtgaacgc atgatcgccg
      541 cccggctcga acggaccccg ctgggccgag ccgccctcgc gaccaggaat cgctggaagc
      601 tgttcacgat ttcggtgctg ctcggtacgg gcacggtgct ggcagcggtg ggtctgctga
      661 ccgcctccgg ctacctgatc agccgtgccg ccgagcggcc ggagatcctt tcgctcaccg
      721 tggcgatcgt cggggtccgc ttcttcgggg tctcccgggc cctgcttcgc tacggcgagc
      781 gcctgacctc gcatgacctg gccttcagaa ccctcaccga cctgcgcggc cggttcttcg
      841 aacgcctgat cccgctggtc ccggccgggc tcgacggctc gcggcgggcc gatctgatga
      901 gccggttcgt cggtgacgtc gaccgcctcc aggatctgta tctgcgggcc ctttcgccgc
      961 cgctggtcgc gatcttctgc tcggtcttct gcgtggcgct cgcctcgatc atcctgccgg
     1021 tggccgggct ggtgctggcc gtgatgctgc tgctcggtgg tctcgcggct ccggccctga
     1081 cccgctgggc ggcacggacg gccggccgcc gccagtccag ggcaaggggc cttctggcca
     1141 tggaccttca tgagattgcc gtcggcggag ccgagatcgc cgtggccggt cgcgaaagcg
     1201 actggctcga gagaagccgg gaggatgacc gccacctcgt ttcactccag cggcgggacg
     1261 cgatgagcgg cggtctggcg gaaggtctcg gaaccctgct ggccgtaggc gccgcggttg
     1321 ccgtcaccgc cgcctcgatt ccggctgtcc attcgggtga gctgaacggc gtgctgctgg
     1381 ccgccctggc tctgctggca atggcctcct tcgaggcgat caccccgctc agccaggcgg
     1441 cggccgtgat tgacgcgacc gacgaagcgg cggaacgcat cgaggaagtg tccgagcgtc
     1501 cggtacccat cctcaaccct gaacatccca gaccggtgcc ggccggcggc ccgatcaggc
     1561 tggacggcgt gagtttcgcc tacccgggag gggacggcct gctgctgcgc gacgccgatc
     1621 tggaaatccg gcccggcgag gcggtcgccc tgatcgggcc cagcgggatc ggcaagagca
     1681 ccctttccga actcctcgtt cgcttcaggg atccggaccg tggacgaata acgctcggtg
     1741 gcgcggacat ccgtgacctg gaccaggagg agttgcgcga agcggtccgg ctggccccgc
     1801 aggacgctta catgttcaac accacgatcg ccgagaacgt caagctgggc aggccggaag
     1861 cggaccgcga gcggatcgtc gaagccctgg ccgaggtcgg cctcgaggac tggatcaact
     1921 cgctgcccga cgggatcgat tccctggtcg gcgaggacgg ctcgatgatc tccggcggcc
     1981 aacgccagcg catcgccgcg gcccgcgtgt tcctttcccg ggcccgcttt ctgatcttcg
     2041 acgagcccac cgcccatctc gacccggacg gtgccgccgc cctggaaagt cacctggtcg
     2101 atcgccgcga tcagggctgc ggcatcctgg tcatcaccca cacgatcgcc gacccgcagg
     2161 ccttcgaccg ggttctcgag cttcgcgacg gaaccttccg cgaagtctga ccgggcgccg
     2221 ggaccgggtt cgctcgcgcc actggcgatc gggatcgatt cgatgcgtcc cgccgggcgg
     2281 ttgccggttc agggctgccg ggccggcaac gaggcggtga tcgtggtgcc atcctcgccc
     2341 gagcgcacgt cgaagtggcc gccggtcagg ttgacccgtt cgcgcatgcc gagcaggccg
     2401 aacccgccgc cgccggcccg gttgggctcg aagccggagc cgtcgtcgat caccctgatc
     2461 cggatctctc cttcttcttc gatcacctcg acgatcgccc gcacggcatg tccgtgccgg
     2521 atcgcattgt tgagtgactc ctggacgagc cggtagacgg tgtcttcgag cagcggctcg
     2581 tagcggccgc ccatttcgtc ttcggggcgg agctcgacat aaaggtcgat ctcgaccccg
     2641 ttgctggaaa gcctttcgac cagtgagctg agcgccgctt ccacgccgag ttcatcgagg
     2701 gcggccggcc ggaggtcggc gatcagccgg cgcagttcga cgatcgtttc gtcgatccgc
     2761 tcgatcgcct ggtcgaggat gggggagacc ggtcccatcg cggtgcgtcg ccgggtgccg
     2821 gaaagcagca cccggaggcc ggccaggttc tgaagggtct cgtcgtgaag ctcgcgggcc
     2881 cagcgtccgc gt
//
LOCUS       DAOQFI010000175         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17696_length_2883_cov_2.213225, whole genome shotgun sequence.
ACCESSION   DAOQFI010000175 DAOQFI010000000
VERSION     DAOQFI010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17696_length_2883_cov_2.213225"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLE93_04055"
     CDS             <1..1072
                     /locus_tag="PLE93_04055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HNH86253.1"
                     /translation="RVATLSGNVVGTADFRTWARCFGLPAPKVSQPGMPSGVYDTQSD
                     PDETVLDVEALSFLAPKLDRVLPIFVPQDNKFNNPLLLFLFGALDPARLGGKLPDILS
                     ISDGVCEYQFRKAEKWLAQRLLAEAAAIGITTLAASGDAGFLGCDLQKPGAQFPASSR
                     FVTAVGGTNLGLDAGNSILSQPVWSTYAKKPSQGAGTGGGPSRFWPRPAFQVAPGLTP
                     ALQKGRKTRLLPDLAAMASFSPGIATFEKGTGGWGPGGGTSAATPLTAAIVALVLQQE
                     KEAGRPPLGLITPLLYKLSQGGDYSQYFRDVKVGTSSRKPKTAVAKTPAGGAAQPGYD
                     LATGLGSLRATALADAVAAMAP"
     gene            1107..2423
                     /locus_tag="PLE93_04060"
     CDS             1107..2423
                     /locus_tag="PLE93_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNH86254.1"
                     /translation="MALRSNQNLPPGPRLPSPMQTFGWWARPTGFLDRARRRYGKSFT
                     VRLAAQDPFIVVSDPDEIKQVFTAPPDVLHPGEGTRILEPIVGPNSILLLDEGAHMTQ
                     RKLVLPAFHGKRMAGLEPLVADVTREQIAAWPRGASAPIHPRMQRLTLEVILAAVFGV
                     RTGERLDPLRQSLTEMLDFGMNPLSLLPPARRAPFGRGPWAKFLKVRARTDELIYGEI
                     QDRRDRGDEGGSEIINSLIGAHHEDGSPMTDEEIHDELMTLLVAGHETTATQLAWTIE
                     RLTREPRVLETLVEAVDNGDGDPYLTAVIRESLRLRPVLLNAAPRTVKKPIEVGGRTY
                     VPRVHLLASAWLVQHDADIYPNPYAFQPERFLESDPGTYTWIPFGGGRRRCVGATFAQ
                     LEMAIVLRELLRTTEIRAGVDGPEATRRRAITVTPGRGAETILTGR"
     gene            2450..2722
                     /locus_tag="PLE93_04065"
     CDS             2450..2722
                     /locus_tag="PLE93_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86255.1"
                     /translation="MVVDPAVTGLHGDHGTVSNRDADGGPGRPIRDGGRGYSCIAEQR
                     TVVSLRHGAPRTPFLRFGETVRIEMRDAAGLSIFGAIEQVLRRRGD"
BASE COUNT          457 a         1044 c          957 g          425 t
ORIGIN      
        1 ccgggtggca accctgagcg gcaacgtggt cggcactgcg gatttccgga cctgggccag
       61 gtgcttcggc ctgccggccc cgaaggtgag ccagccgggc atgccgagcg gcgtgtacga
      121 cacccagagt gacccggacg aaaccgtgct cgatgtcgag gcgctttcct tcctggctcc
      181 gaaactggac cgcgtcctgc cgatcttcgt accgcaggac aacaagttca acaacccgct
      241 gctgctcttc cttttcggtg cgcttgaccc ggcccggctg ggaggcaagc tcccggacat
      301 cctctcgatc agcgacgggg tctgcgaata ccagttccgc aaggccgaga agtggctggc
      361 ccagcgcctg ctcgcggagg cggcggcgat cggcatcacc accctcgccg cctcgggtga
      421 cgcgggcttc cttggctgcg acttgcagaa gcccggggcc cagttcccgg ccagttcccg
      481 gttcgtaacc gcggtcggcg gcacgaatct cgggctcgat gccggcaact cgatcctctc
      541 ccagccggtc tggtccacct acgcgaagaa gccttcgcag ggtgccggaa cgggcggcgg
      601 acccagccgg ttctggcccc ggccggcctt ccaggtcgcg cccggcctca cccccgccct
      661 gcagaagggc agaaagaccc ggcttttgcc ggatctcgcc gcaatggcat ctttcagccc
      721 cggcatcgcc accttcgaga agggcaccgg cggctgggga cccggtggcg gtaccagcgc
      781 cgcaacgcca ctcaccgcag cgatcgtcgc gctcgtcctg cagcaggaaa aagaagccgg
      841 acggccgccc ctcgggttga tcaccccgct gctctacaaa ctcagccagg gcggtgatta
      901 cagccagtac ttccgcgacg tcaaggtcgg caccagttcc cgcaaaccga agaccgcagt
      961 cgccaagacc ccggccggcg gtgccgccca gccgggctat gacctcgcga ccggccttgg
     1021 ctcgctgcgg gccaccgcct tggccgacgc ggtcgccgcg atggcacctt gaccacaccc
     1081 ggtcctgcag gcgtaagctt tctttcgtgg ccctgaggtc taaccagaac ctgccccccg
     1141 gtccccgcct gccgagcccg atgcagacgt tcggctggtg ggctcgacca accggtttcc
     1201 tcgacagggc gaggcgcagg tacggcaaga gcttcacggt ccggctggcc gcccaggatc
     1261 cgttcatcgt ggtctccgac ccggatgaga tcaagcaggt cttcaccgct cccccggacg
     1321 ttctccaccc cggagaaggc acccggattc ttgaaccgat cgtcggcccg aactcgatcc
     1381 tcctcctcga cgagggtgct cacatgacgc agcgcaagct ggtcctgccg gcctttcacg
     1441 gcaagcgcat ggccggtctc gaaccgctcg tcgcggacgt gacccgggag cagatcgcag
     1501 cctggccgcg gggcgcatcg gccccgatcc acccgcgcat gcagcggctc accctcgagg
     1561 tgatcctcgc cgccgtgttc ggagtccgga ccggtgagcg actcgacccg ctgcgccagt
     1621 cactgaccga gatgctggat ttcggcatga acccgctcag cctgctgccg ccggcccggc
     1681 gggccccgtt cggccggggt ccctgggcga agttcctcaa ggtccgggcc aggaccgacg
     1741 agctgatcta cggcgagatc caggatcggc gcgaccgcgg cgacgagggc ggcagcgaga
     1801 tcatcaactc gctgatcggc gcccaccacg aagacggttc gccgatgacc gacgaggaga
     1861 ttcacgacga gctgatgacc ctgctggtgg cgggtcacga aacgaccgcg acccagctcg
     1921 cctggacgat cgaaagactg acccgcgagc cccgggtcct ggaaacgctg gtcgaggcgg
     1981 tcgacaacgg tgacggcgac ccctacctga ccgcggtgat ccgcgagagc ctgaggctgc
     2041 ggccggtgct gctgaacgcc gccccccgca cggtcaagaa gccgatcgag gtcggcggcc
     2101 ggacctatgt gccgcgggtt cacctgctgg ccagcgcctg gctggtccag cacgacgccg
     2161 acatctaccc gaacccctac gccttccagc ccgaacgctt cctcgagtca gatcccggca
     2221 cctacacctg gattcccttc gggggtggtc gtcggcgctg cgtcggagcc acgttcgcgc
     2281 aactggaaat ggcgatcgta ctgcgcgaac tgctccgcac caccgagatc cgggccggtg
     2341 tcgacgggcc ggaagcaacc cgccgccggg cgatcaccgt gaccccgggc cgcggagccg
     2401 aaacgatcct gaccggccgc tagggcaggg cgatgaagcg gatgcggtag tggtcgtgga
     2461 ccctgccgtc acgggtcttc atggcgatca cggcaccgtc tcgaaccggg atgccgatgg
     2521 cggcccgggc cgaccgatcc gcgacggcgg ccgcggctat tcctgcatcg ccgagcagcg
     2581 cacggtcgtg tcgctgcgcc acggtgcgcc gcgcacgccg ttcctgcgct tcggcgagac
     2641 ggtgcgcatc gagatgcgcg acgcggcggg cctgtcgatc ttcggcgcga tcgagcaggt
     2701 gctgcgccgt cgcggcgact gaacacgcgg ccgcgaggcg ccaggggcac gcgggcgcat
     2761 cgatatactg gccacgggat cccctagcgg aagcgcgcca tggcccgtcg tcccgtcgcc
     2821 gtcgggcctg tcgcgggccg gcagcgcgtc gggggcgggc accggtgagc cggcgacgag
     2881 gac
//
LOCUS       DAOQFI010000176         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17731_length_2879_cov_3.750000, whole genome shotgun sequence.
ACCESSION   DAOQFI010000176 DAOQFI010000000
VERSION     DAOQFI010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17731_length_2879_cov_3.750000"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..2070
                     /locus_tag="PLE93_04070"
     CDS             67..2070
                     /locus_tag="PLE93_04070"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNH86256.1"
                     /translation="MGEIGGALNRSVSRSVALALFGIAALFAGDDEAPASTQNLNSTC
                     GNTKVSTACSDTVYGALHNNGEAKVGPRRLVVNQSGKIFSRGTRLFVAHTPLDIGSET
                     VRIKKTDGKAKVSVAICSTAAADPFTQRVESFFTIPSGKGSIGTTFSRTVSGIKNRRL
                     SVRFAGKSLTDNIKYTMTVDRPGDDEIWMPSQTQLNSVYRPVEGFADLHAHHAGGLAF
                     ASGWYIGGMEQGSIYSGDHQKKLKVNLVGVPLTTLRPAHQGKVIPSSDDNSHQQMNLN
                     ALKAAHDNGLGLMVSPAVNSEWLCSVLAYALEINKRQSCQDMESAKYQILKMKQFDEK
                     YGWYDIVTNPWEARSAAYAGRLPVVLGVEVSDLFPKSDGDWRRQLAELYDMGVRVVYV
                     AHESNSRFAGAAYHHTEDLLLPNELKAWFSKEIKFASMKNGRNAIGLSDLGEDLIREL
                     MRRRMLIDVDHASWKAVDEIFDITSANRYYPIYAGHTRPYELLTDYMKYLVPELNTPP
                     RVLDYIKRSGGMVALRTGPEGMETYSESGVANNCSGSVRSLIQMYRYFVDEDVPVAFG
                     SDFNGFVEEIGSRFGRTGCPASTGEFRELQLTSQTRPSSAGAPPSWQRYLDRGMSDIG
                     TEPAVVFDMQKLGVDTAPLENSAQSFLQMWGRAYRSNRTEVQP"
     gene            complement(2554..>2879)
                     /locus_tag="PLE93_04075"
     CDS             complement(2554..>2879)
                     /locus_tag="PLE93_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fenitrothion hydrolase"
                     /protein_id="HNH86257.1"
                     /translation="HYFSLFFFQEQAQFTYLLSDPLGTGTTDLFGTASYGVNYNAISN
                     QVIWYVQVAALVCGHVAGLVLAHDKAITIWKDYRTAARSQYWMLAVMVAFTCFGLFLL
                     SVSNS"
BASE COUNT          564 a          905 c          926 g          484 t
ORIGIN      
        1 gtcctcctcg gaatcctttc ctgagaattt ccccgcagcc cgaccgtccg cggttagtct
       61 cgtctcatgg gggaaattgg gggagcactc aaccgatcgg tcagccgctc ggtggcgctg
      121 gcactttttg ggatcgcggc acttttcgcg ggagatgacg aagccccggc aagtacccag
      181 aatctcaatt cgacctgcgg caacacgaag gtctcgaccg cgtgttcgga cacggtttac
      241 ggcgcgctgc acaacaacgg cgaggcgaag gtcgggcccc gtcgcctggt cgtcaaccag
      301 tcgggaaaga tcttctccag gggcacccgg ctcttcgtcg cccacacccc gctggacatc
      361 ggctccgaga ccgtgcggat caagaagacc gacggcaagg ccaaggtcag cgtcgcaatc
      421 tgttcgaccg cagccgccga ccccttcacg cagcgggtcg agagtttctt caccatcccg
      481 agcggcaagg gaagcatcgg caccactttc tcccggaccg tcagcggcat caagaaccgg
      541 cggctctcgg tgcggttcgc cggcaagagc ctcaccgaca acatcaagta cacgatgacc
      601 gtggaccgcc cgggcgatga cgagatctgg atgcccagcc agacgcagct caactcggtc
      661 tatcgcccgg tcgaaggctt cgccgacctt cacgcgcatc atgccggcgg actcgccttc
      721 gccagtggct ggtacatcgg cggcatggag caggggtcga tttattccgg ggatcaccag
      781 aagaagctga aggtgaatct ggtcggggtt ccgcttacca cgctgcggcc ggcccatcag
      841 gggaaggtga ttccgagctc ggatgacaac agccatcagc aaatgaacct caacgcgctg
      901 aaggcggccc atgacaacgg gctcggattg atggtctccc ccgccgtcaa cagcgagtgg
      961 ctctgttcgg tgctcgccta cgcgctcgag atcaacaagc ggcagtcctg ccaggacatg
     1021 gagtcggcga agtaccagat cctcaagatg aagcagttcg acgagaaata cggctggtat
     1081 gacatcgtca ccaatccctg ggaggcccga tcggcggcct acgcgggccg cctgccggtg
     1141 gtgctgggag tcgaggtgag cgacctcttc cccaagtccg acggggactg gcggcgtcag
     1201 cttgccgagc tctatgacat gggcgtccgg gtcgtctacg tggcccatga gtcgaacagc
     1261 cgtttcgccg gcgccgccta ccaccacacc gaggacctgc tgcttcccaa cgaactgaag
     1321 gcctggttct cgaaggagat caagttcgct tcgatgaaga acggccgcaa cgcgatcggg
     1381 ctcagcgacc tcggcgagga cctgatccgc gagctgatga ggaggcgcat gctgatcgac
     1441 gtcgatcacg cctcctggaa ggcggtagac gaaatcttcg acatcaccag cgccaaccgc
     1501 tactacccga tctacgcggg ccacacgcga ccctacgagc tgcttaccga ctacatgaag
     1561 tacctcgtgc cggagctcaa caccccgccc cgggtccttg actacatcaa gcggagtggc
     1621 ggcatggtcg ccctgcggac cggacccgaa gggatggaga cctacagcga aagcggcgta
     1681 gccaacaact gctcgggctc ggtccggtcg ctgatccaga tgtaccggta cttcgtcgac
     1741 gaagacgtgc cggtcgcctt tggcagtgac ttcaacgggt tcgtggaaga gatcggatcc
     1801 aggttcgggc ggaccggctg cccggcctcg accggcgagt tccgggaact gcagttgacc
     1861 tcccagaccc gcccctcctc cgccggtgcg ccgcccagct ggcagcgcta cctcgaccgg
     1921 ggaatgtcgg acatcggcac cgaaccggcg gtcgtcttcg acatgcagaa actcggggtc
     1981 gacaccgcac ccctcgagaa ctcggcccag tccttcctcc agatgtgggg ccgcgcctac
     2041 cggagcaacc gcaccgaagt ccagccctga gctgaaaccg gccggcggca ttgagggcga
     2101 agttgttggc gtcccagcgt cccgcaccgt ctccagggcc aggtccaggc ccaggtttcg
     2161 ggaggtgggt atggatttcg tctatatgaa cgaaatccat acccaccagc cccggatccg
     2221 ggtcgggaaa tcgatcgaga tccgatctgt cagcgctatt tactgacaaa ccgcatctcg
     2281 cgaaaacggg ttcaccacca accgtgagcg ggtcgtgggc ctccagccgt cagcgggcca
     2341 aacttcggcg ggtggatgcc agggacctcc agccgtgagt gggtcgtggg cctccagccg
     2401 tcagcgggtc gtgcacaggg cgctcctgcg ggtggctggc gaggaataag ccgggaaatg
     2461 gccgcgatcg tacgcgggta cttgagctgc cgtttcccgg cgttgacgaa gccacggcgc
     2521 ccgcaggtgc gtcctgggcg cgttgcccgg gccttaactg ttcgagactg acagtagaaa
     2581 gagcccgaaa caggtgaagg cgaccatcac ggcgagcatc cagtactggg agcgggcggc
     2641 ggtgcggtag tccttccaga tggtgatcgc cttgtcgtgg gcgaggacga ggccggcgac
     2701 gtggccgcag acgagggcgg cgacctggac gtaccagatc acctgattgg agatcgcgtt
     2761 gtagttgacc ccgtaggaag cggttccgaa gaggtcggtg gtgccggttc cgagcggatc
     2821 ggagagcaga taggtgaact gggcctgctc ctggaagaag aagaggctga agtagtggg
//
LOCUS       DAOQFI010000177         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17867_length_2868_cov_3.098827, whole genome shotgun sequence.
ACCESSION   DAOQFI010000177 DAOQFI010000000
VERSION     DAOQFI010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17867_length_2868_cov_3.098827"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PLE93_04080"
     CDS             complement(<1..1323)
                     /locus_tag="PLE93_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNH86258.1"
                     /translation="MRLQRPERRIGLLFAVSLVLLLGLFARAFLLQVVQGSSLASQAK
                     GQQEDIIEVPGLRGSILDRNGQPLAGSEAGATIYATPYQIKDPPEEARQVAKALDADP
                     GEVLDAITQPGGFSYVDRKVDLKKAEAVDKLGLEGIGQVPDTLRVRPQGDIASQVIGA
                     VSDEGEGLTGIEAGQDGVLKGVNGERRLVKDALGDPISLDNVRDAEDGQPVQLTLDSN
                     IQAEAEKVLAGIGETSNPINASAIVMDAQSSDVLAMANWPPVDLDNLSQAKPEDLLNT
                     GTSYTYEPGSTFKAITVASALETGTVTPSSTFTLAPSIQVFDRVIEESHPRGTVTLDV
                     GDILAQSSNVGAVTIGLEMGATKFSRWIGRFGFGKPTGIDYPGEEQGIVLPYSDWSGS
                     TIGNVPIGQGLAVTPIQMVQAYGALANGGILRPPRLIEKIGGEEVPEQK"
     gene            complement(1323..1808)
                     /locus_tag="PLE93_04085"
     CDS             complement(1323..1808)
                     /locus_tag="PLE93_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86259.1"
                     /translation="MGAPARTIPVLPARVPSRRTVPHRRPATRRRPGSRQHSGLRLIG
                     RSAHAVTHFPESGLVRTAATGRRWIFVIGALLLAIVAINVVTVSYGAMASKLETQIES
                     IERQNAILKSSQTAALAMPRVQAQAEAEGMYLPEPDAIHYRSFQNSDYAAAAERLAAG
                     G"
     gene            complement(1808..2854)
                     /gene="rsmH"
                     /locus_tag="PLE93_04090"
     CDS             complement(1808..2854)
                     /gene="rsmH"
                     /locus_tag="PLE93_04090"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNH86260.1"
                     /translation="MTAAARLAPIGAKVSDSRQSHNPFDRPEQGPRGHLPVLAGELVD
                     LIGPQLGETVIDCTFGAGGHARLVAERIGAEGTLIAIDRDPVAQAHFQAFETEAACRT
                     RFVAAEFSQALKYLQNEGIRASALYMDLGMSSLQVDAAERGFSYSHDAPLDMRMDPRQ
                     ELTAEVIVNTWSEQQLAQMLRELGEERYAGGISRQIVARRPLTSTSDLVEAIKAGMPA
                     AARFGSGHPARRSFQALRIAVNGELEAVDAALPIAWDSLEIGGRMAVISFHSLEDRRI
                     KRFFNDLATGCTCPPELPVCVCGHEPEAEVLTRRPISAGEEEVGMNPRARSARLRGAL
                     KLREANENPAGEVR"
BASE COUNT          444 a          956 c          972 g          496 t
ORIGIN      
        1 cttctgctcc ggaacctcct cgcccccgat cttttcgatc agccgcgggg gccggagaat
       61 gccgccgttg gcgagagccc cgtaggcctg gaccatctgg atcggggtga ccgccaggcc
      121 ctggccgatc ggcacgttgc cgatcgtcga accggaccag tcgctgtaag gaaggacaat
      181 cccctgttcc tcgccggggt agtcgatccc ggtcggcttg ccgaaaccaa agcggccgat
      241 ccagcggctg aacttcgtcg cccccatctc gagcccgatc gtcaccgccc ccacgttcga
      301 ggactgggcg aggatgtcac cgacatcgag ggtcaccgtt cctcgcggat ggctctcttc
      361 gatcacccgg tcaaacacct ggatcgaggg ggcaagtgtg aaagtgctgg acggggtgac
      421 agttccggtc tcaagcgccg aggcgaccgt gatcgccttg aaagtcgagc cgggctcgta
      481 ggtgtacgag gtgccggtgt tgagcaggtc ttcgggtttc gcctgactga gattgtcgag
      541 gtcaaccggg ggccagttgg ccatcgccag cacgtcggaa ctctgcgcgt ccataacgat
      601 cgccgaggca ttgatcgggt tgctggtctc gccgatcccg gccagcacct tctcggcctc
      661 ggcctggatg ttcgagtcca gcgtcagctg aaccggctgg ccgtcctcgg cgtcgcggac
      721 gttgtcgagg ctgatcgggt cgccgagtgc gtccttgacc agccgccgct ccccgtttac
      781 gcccttgagc acgccgtcct gtccggcttc gattccggtc agtccctcgc cttcgtcgct
      841 gaccgccccg atcacctggg aggcgatgtc accctggggc cggacccgca gggtgtccgg
      901 aacctgtccg atcccttcga ggcccagctt gtcgaccgct tcggcctttt tcaggtccac
      961 cttgcgatcg acgtatgaga acccgccggg ctgggtgatc gcatcaagca cctcgcccgg
     1021 atcggcgtcg agcgccttcg cgacctgcct ggcctcctcc ggcggatcct tgatctggta
     1081 cggggtggcg taaatcgtgg cgccggcttc ggacccggcc agcggctggc cgttgcggtc
     1141 gaggatcgag ccgcgcagac cgggcacctc gatgatgtct tcctgctggc ctttcgcctg
     1201 ggatgcgagc gaggaaccct gaaccacctg aagcagaaaa gcccgggcga agagcccgag
     1261 cagaagaacc agactgaccg cgaagagcag gccgatccgc cgttcgggcc gttgcagtcg
     1321 catcagcccc cggccgccag tcgctcggcg gccgccgcat agtcggagtt ctggaaggag
     1381 cggtagtgaa tcgcgtcggg ctctggcagg tacatgcctt cggcttcagc ctgggcctga
     1441 acgcggggca tcgccagagc agccgtctgc gacgacttca ggatggcgtt ttgccgctcg
     1501 atcgattcga tctgggtttc gagcttgctg gccatcgcgc cgtagctgac ggtgaccacg
     1561 ttgatcgcga cgatcgccag tagcagcgcg ccgatcacga agatccagcg tcggccggtg
     1621 gcggcggtgc gaaccagccc cgactcgggg aaatgagtga ccgcatgggc gctcctgcca
     1681 atcaggcgca ggccggagtg ctgccggctg ccgggccggc gccgggtggc gggccgacgg
     1741 tgagggaccg tgcgccggga gggaacgcga gcgggaagga ccgggatggt gcgagccggg
     1801 gcgcccatca gcgaacctcc ccggcgggat tctcattcgc ttcacgcagc ttcagggccc
     1861 ccctcaggcg ggccgagcgg gccctcgggt tcatcccgac ttcttcctcc ccggcggaaa
     1921 tcggccggcg ggtgagcacc tcggcctccg gctcatgccc gcagacgcag accggcagct
     1981 ccggcgggca ggtgcagccg gtcgcgagat cgttgaagaa gcgcttgatc cggcggtctt
     2041 cgagcgagtg gaacgagatg acggccatgc gaccgccaat ctcgaggctg tcccaggcga
     2101 tcggcagggc cgcatcgact gcttcgagtt caccgttgac cgcgatccga agcgcctgga
     2161 agctgcggcg ggccggatgg ccggaaccga aacgggccgc ggccggcatc ccggccttga
     2221 tcgcctcaac caggtccgaa gtgctggtca gcggccgccg ggcgacgatc tggcggctga
     2281 tcccgccggc gtatcgctcc tcgccgagtt cacgcagcat ctgggcgagc tgctgctcgg
     2341 accaagtgtt gacgatcact tcggcggtca attcctgtcg cggatccatc cgcatgtcga
     2401 ggggcgcgtc atgtgagtag gagaagccac gctcggcggc gtccacctga agtgaagaca
     2461 tcccaagatc catgtacagc gccgaagcgc gaataccttc attttgcagg tatttcagag
     2521 cctggctgaa ctcggcggcg acgaatctcg tccggcaggc ggcctcggtc tcgaacgcct
     2581 ggaagtgagc ctgggcgacc gggtcacggt cgatcgcgat cagggtacct tcggctccga
     2641 tccgctcggc gaccaggcgg gcgtgaccgc cggccccgaa tgtgcagtcg atcaccgtct
     2701 cgccgagctg cgggccgatc aggtcgacca gctccccggc cagtaccggc aggtgccccc
     2761 tcggcccctg ttcgggccgg tcgaaagggt tgtggctctg gcgcgagtcg ctcaccttgg
     2821 ctccgatcgg ggcgagccgc gcggcggctg tcactccgtg ctcccggc
//
LOCUS       DAOQFI010000178         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17897_length_2865_cov_3.777224, whole genome shotgun sequence.
ACCESSION   DAOQFI010000178 DAOQFI010000000
VERSION     DAOQFI010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17897_length_2865_cov_3.777224"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PLE93_04095"
     CDS             complement(<1..1325)
                     /locus_tag="PLE93_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNH86261.1"
                     /translation="MPAPKKNLITLTVDGREIEAIEGTMLVDAAKHGDIEIPVFCYEP
                     KLGAPVGACRMCLVEIEGIPKLQTACSTPVRDGMVVHTRTDQVKEAQNAVVEFLLVNH
                     PLDCPVCDKGGECPLQDISMGWGPDRSRVVDTKRHFEKPVPLSPLIAIDRERCILCYR
                     CARFSQEVSEDGQLQLLDRGAESYIGTFDDRPYIAPFQGNITELCPVGALTSYTYRFQ
                     ARPWDIEQGGSVCTLCPSQCNVSFTIRDEQVKRVLDRENDQIDDGWLCDRGRYGFQAM
                     YSQDRVMNPLRYIDGHPAPARWSDALEAASIGLAAAEGKVAALVGDASNEEAFILQDL
                     IRNGLGSADIDSRPAPTVGRDQRIRLDDPATQARVVDIDHADVVLVLGNDPIHSAPAF
                     DLRVRKAVRRNGTALAVATERPSALDGGASEVERIKPGDAAGYLANLLD"
     gene            complement(1325..2734)
                     /gene="nuoF"
                     /locus_tag="PLE93_04100"
     CDS             complement(1325..2734)
                     /gene="nuoF"
                     /locus_tag="PLE93_04100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNH86262.1"
                     /translation="MSVAETRVLLRHAEDPKMATLEGYESYGGYATLKKALNEIDPEW
                     IVTELEESGLRGRGGAGFSMGKKVSFLPRTEDAKYLCCNADESEPGAFKDRELMQRNP
                     HQLIEGIIISSFGANIGHAFIFIRGEYDDQADILDRAVDEAYEAGYLGTNILGSGFNL
                     ELVVHRGAGAYICGEETALLDALEGKRGNPRLKPPFPAVQGLYGGPTLINNVETLSNV
                     SHIVANGADWFKSFGTEQSPGTKVVSVSGCVKRPGNYEVELGVTLRDLIYGLAGGPLD
                     GHEITAFYPGGSSSPVLTPAELDLPYSFEAMAEAGSMLGSGSIIVADDSVSIPRMALR
                     TAHFYRHESCGKCTPCREGTNWTEKMLERVCAGEATPMDLDIIESVQHNIMGHCLCVL
                     GDAMAMPVSSMVNKFRDEFEETIAFEAARAWDGSLDSSASPGRDVPSGDEPGPGGESP
                     EKIRRERLAGRAIPDGSGR"
BASE COUNT          470 a          903 c          985 g          507 t
ORIGIN      
        1 tggtcgagca ggttcgcaag gtacccggcc gcgtcacccg gcttgatgcg ctcgacttcg
       61 gaggcgccgc cgtcgagggc ggaaggccgc tcggtggcga ccgccagcgc ggtgccgttg
      121 cgacggaccg ccttgcgaac ccgcaggtca aaggccggag ccgagtggat cgggtcgttg
      181 cccagtacca gcaccacatc ggcgtggtcg atgtcaacga cccgggcctg ggtggcaggg
      241 tcatcgaggc ggatccgctg gtcgcggccg accgtgggag ccggccgtga atcgatgtca
      301 gcggagccga ggccgttccg gatcaggtcc tgcaggatga aagcctcttc gttggaagcg
      361 tctccgacca gggcggcaac cttgccttcg gcggcggcga gaccgatcga agccgcttcg
      421 agggcgtcgc tccaccgggc cggggccggg tggccgtcga tgtaacggag cgggttcatg
      481 acccggtcct gcgaatacat cgcctggaag ccgtagcggc cacggtcaca gagccagccg
      541 tcgtcgatct ggtcgttttc acgatcgagc acgcgcttca cctgctcgtc gcggatcgtg
      601 aagctgacgt tgcattggct cgggcagagg gtgcagaccg agccgccctg ctcgatgtcc
      661 cacggccggg cctggaaacg gtaggtgtag gaggtcaggg cgccgaccgg gcagagctcg
      721 gtgatgttgc cctggaaggg cgcgatgtac ggccggtcat cgaaggtgcc gatgtaggac
      781 tcggcgcccc ggtcgagcag ttgcagctgg ccgtcctcgg aaacttcctg gctgaaacgg
      841 gcgcagcggt aacagaggat gcagcgttca cggtcgatcg cgatcagtgg tgagagcggg
      901 accggtttct cgaaatgtcg cttggtgtca accacacggc tgcggtcggg gccccagccc
      961 atcgagatgt cctggagcgg gcactctccg cccttgtcac agaccggaca gtcgagcgga
     1021 tggttgacca gcaggaactc gacgaccgcg ttctgggctt ccttgacctg gtcggtccgg
     1081 gtgtgaacga ccatgccgtc gcggaccggt gtcgagcagg cagtctggag tttcgggatg
     1141 ccctcgatct cgaccaggca catgcggcag gcgccgaccg gcgcgccgag cttcggctcg
     1201 taacagaaga ccgggatctc gatgtcaccg tgcttggccg catcgaccag catcgtgcct
     1261 tcgatcgcct cgatctcccg gccatcgacc gtaagggtga tcagattctt cttcggagcc
     1321 ggcatcagcg accgctcccg tccggaatcg cgcggccagc caaccgctcc cgccggatct
     1381 tctcgggact ctcgccacca ggcccgggct catcgccgga cggaacatcc cgaccgggag
     1441 aagcagacga gtccagggaa ccgtcccagg ctcgcgcggc ttcgaatgcg atcgtctcct
     1501 cgaattcgtc gcggaacttg ttgaccatcg agctgaccgg catcgccatg gcgtcaccga
     1561 ggacacagag acaatggccc atgatgttgt gctggaccga ttcgatgatg tcgaggtcca
     1621 tcggggtggc ctcgccggca cagacccgtt cgagcatctt ctcggtccag ttggtgccct
     1681 cgcggcaggg cgtgcacttg ccgcaggatt cgtgacggta gaagtgagcg gtccggagcg
     1741 ccatcctcgg aatcgagacc gagtcgtcgg cgacgatgat cgacccggag ccgagcatcg
     1801 acccggcctc ggccatcgcc tcgaaggaat aaggcaggtc aagttcggcc ggggtcagca
     1861 ccggtgagga ggaacctccc gggtagaagg cggtgatctc gtggccgtcg agcgggccgc
     1921 cggccaggcc gtagatcagg tcgcgcaggg tgacgccgag ctcgacctcg tagttgccgg
     1981 gtcgtttgac gcagccggaa accgaaacga ccttggtgcc gggtgactgc tcggtgccga
     2041 agctcttgaa ccagtcggcg ccgttggcga cgatgtgact cacgttgctc agggtctcga
     2101 cgttgttgat cagggtcggc ccgccgtaga ggccctggac cgcagggaag gggggcttga
     2161 ggcgcgggtt gccgcgcttg ccttccagcg cgtcgagcag ggcggtttcc tcgccgcaga
     2221 tgtaggcccc ggcaccgcgg tggacgacca gctcgaggtt gaagcccgaa ccgaggatgt
     2281 tggtcccgag gtaacccgcc tcataggcct catcgaccgc ccgatcgagg atgtcggcct
     2341 gatcgtcgta ctcgccgcgg atgaagatga aagcgtggcc gatgttggcc ccgaaactgg
     2401 agatgatgat gccttcgatc agctgatgcg ggtttcgctg catcagctcg cgatccttga
     2461 aggcccccgg ctcggattcg tcggcgttgc agcagaggta cttggcatcc tctgtacgcg
     2521 gcaggaagga caccttcttg cccattgaga atccggcacc gccgcgaccg cgcaggcccg
     2581 attcctcgag ctcggtcacg atccactcgg ggtcgatctc gttcagcgcc ttcttcaggg
     2641 tcgcgtagcc gccgtaggac tcgtaaccct cgagggtcgc catcttcggg tcttcggcgt
     2701 ggcggagcag cactctggtt tcagcgacgc tcactgattc ggcccctttc cgccggtcac
     2761 ccgggggtcg gtcgaatcgg cgacgccgcc ggccaggccg cgcttgtcca gcgccttctc
     2821 cggcaggact tcctcgccgg attcgagctg atcgacgatg gtggc
//
LOCUS       DAOQFI010000179         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17919_length_2863_cov_2.920584, whole genome shotgun sequence.
ACCESSION   DAOQFI010000179 DAOQFI010000000
VERSION     DAOQFI010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17919_length_2863_cov_2.920584"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLE93_04105"
     CDS             complement(<1..489)
                     /locus_tag="PLE93_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86263.1"
                     /translation="MTLALFAATLVSLLAASPSVGALVPRFALDGSPGPSGSYPFDLT
                     DLNGTLLFSAEIERGNELYATSDGRKIRLVRDLLPGPDDGDPSDFTRFGNFVYFLGYE
                     KNAGYEMWRTNGRSASLFAEPSPGPDSSNIDFLTVADGFLYFSAETPAAGRELWKTDG
                     QAT"
     gene            501..1877
                     /locus_tag="PLE93_04110"
     CDS             501..1877
                     /locus_tag="PLE93_04110"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNH86264.1"
                     /translation="MCFQLRASFPRSSASAPTLAPARRAVKRPRSNRYASVMEVTAAD
                     IAADTVRAKSAARAMAAAPTGVKNRALEAIARGLGESVDGILAANASDLADERAQGLS
                     RALTDRLTLTPERVGAMAAGVREVAALDDPVGEVLESRTLENGLELSKIRVPLGVVAV
                     VYEARPNVTIDCAALTIKSGNSILLKGSSYAENTNLALAEVVRSGLAEAGLPEDGVVM
                     SVGGGRESIADLATQDGVVDLLIPRGGEGLKKTLEEVATVPVIFAAAGICHVYVHSDA
                     NLEMARAIAYNAKIQRPGVCNAAETLLVDSAVAAAFLPPALAELSGGGVELVGDESTR
                     ENAGDTPVGEATEVDWDTEYLDLKLAVKVVDGLDEAIEHINRHGSGHSDAIVTDNAQA
                     ADLFAQGVDSAAVYVNASTRFTDGFEFGMGAEIGVSTQKLHARGPVGLKELTTTKYVG
                     RGDGQVRG"
     gene            1879..2484
                     /gene="nadD"
                     /locus_tag="PLE93_04115"
     CDS             1879..2484
                     /gene="nadD"
                     /locus_tag="PLE93_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNH86265.1"
                     /translation="MTRLGVFGSSFNPPTLAHAVLLAEAGWQLALDRIMVVPTGEAWH
                     KDISGAPAAGVRMALAKAAFGGQEGLEVSSLEVDRQGPSYTCDTLEEIHSLNPDSQVF
                     FLSGSDAALGLGEWHRPDRVLELAEFAVAPRSEIARDAVAEVFERLGARDRLEFFAMP
                     RMEFSSTLVRERIASNRPWKHLVPQGVAEMIDNEDLYGSKQ"
     gene            2468..2854
                     /gene="rsfS"
                     /locus_tag="PLE93_04120"
     CDS             2468..2854
                     /gene="rsfS"
                     /locus_tag="PLE93_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNH86266.1"
                     /translation="MAASSEELSRDIARLLDQKLGEDIVCLDMRELVTYTDYLVICTA
                     RNERQAEAIADEVHLRLKHDNSLLPVNPDRSGDVGWTVLDYLDCVLHIFTEEARQRYD
                     LEDLWHEAPHLDLGLDQSGAKSASAA"
BASE COUNT          517 a          919 c          953 g          474 t
ORIGIN      
        1 cgtcgcctgg ccgtcggtct tccagagctc ccttccggca gccggggttt cggcggagaa
       61 gtagaggaaa ccgtcggcca cggtcaggaa gtcgatgttc gatgagtccg gtccggggga
      121 aggctcggcg aacagtgaag cgctcctgcc gttggttctc cacatttcgt acccggcgtt
      181 cttctcgtag ccgaggaagt agacgaagtt gccgaatctg gtgaagtcgg aaggatcccc
      241 gtcgtctggt cctggcagca aatcccggac cagcctgatc ttcctgccgt cactcgttgc
      301 gtacaactcg ttgccgcgct cgatctcggc cgaaaacagc agggtgccgt tgaggtcggt
      361 caggtcgaac ggataagacc cgctgggccc gggactgcca tcaagggcaa agcggggaac
      421 aagggcgccc accgaggggg aggccgccag cagcgacacc aaagtggcgg cgaacaaggc
      481 cagcgtcaac cggaccttca atctgtttcc aactccgtgc aagcttcccc cgtagcagtg
      541 cgtctgcccc gacgctagcg cccgcacggc gcgcggtcaa gcggcctcga tcgaacaggt
      601 acgcttcagt tatggaagtg accgccgccg acatagccgc cgatactgtc agagccaaaa
      661 gcgccgcccg cgccatggcc gcggctccga ccggggtcaa gaaccgggcg ctcgaggcga
      721 tcgcccgtgg tctcggtgaa tcggttgacg ggattctcgc cgcgaacgct tcagatctgg
      781 ccgacgaaag ggcccagggg ctttccaggg cgctgactga ccggcttacg ctcacgcccg
      841 agcgcgtggg agcgatggcc gccggggtgc gggaggtcgc tgccctcgac gacccggtcg
      901 gggaagtgct ggaatcgcgc accctcgaga acgggcttga gctgagcaag atcagggtgc
      961 cgctcggcgt cgtcgccgtg gtttacgagg cgcggccgaa cgtgaccatc gactgcgctg
     1021 ccctgaccat caagtcaggc aactcgattc tgctcaaggg gtccagctac gccgagaaca
     1081 cgaacctggc gctggccgaa gtcgtgcgat caggcctggc cgaagccggc ctgcccgagg
     1141 acggcgtcgt gatgtcggtc ggaggcggcc gcgagtcgat tgccgacctc gccacccagg
     1201 atggcgtggt cgacctgctg attccccgcg gcggtgaagg gctgaagaag acgctcgaag
     1261 aggtggcgac cgtaccggtg atcttcgctg cggccgggat ctgtcatgtc tacgtccact
     1321 ccgacgcgaa cctggagatg gcccgggcga tcgcctataa cgcaaagatc cagcgccccg
     1381 gcgtctgcaa cgcggccgag accctgctgg tggacagcgc agtcgccgct gccttcctgc
     1441 cgcccgccct ggccgagctt tccggcggcg gagtcgaact ggtcggggac gaatcaaccc
     1501 gcgagaacgc cggggacaca ccggtcggcg aagcgaccga ggtcgattgg gacaccgagt
     1561 acctcgacct gaaactggcc gtgaaggtgg tggacgggct cgacgaagcg atcgagcaca
     1621 tcaaccgcca cggcagcgga cactccgacg cgatcgtcac cgacaacgcc caggcggccg
     1681 atctttttgc ccagggtgtc gattcggctg ccgtctacgt gaacgcttcg acccggttca
     1741 ccgacggatt cgaattcggt atgggagccg aaatcggcgt ttcgacccag aagcttcacg
     1801 cccgcgggcc ggtcggcctc aaggaactga ccacgaccaa gtacgtgggc cgcggcgacg
     1861 gccaggttcg cggctgaagt gacccggctc ggggttttcg gctcttcttt caatccgccg
     1921 acgctggccc acgcggtgct gctcgccgaa gccggctggc agctcgccct tgaccggatc
     1981 atggtcgtgc cgaccgggga ggcctggcac aaggacattt ccggggcgcc cgccgccggg
     2041 gtccggatgg ccctggcgaa ggcagccttc ggcggccagg aagggcttga agtttcatcg
     2101 ctcgaagtgg accggcaggg tccgtcctac acatgtgaca cccttgagga gatccattct
     2161 ttgaatccgg acagccaggt cttcttcctc tcgggctctg atgccgccct ggggttgggg
     2221 gagtggcatc ggcccgaccg cgtgcttgag ctggcggaat ttgccgtggc accgcgttca
     2281 gagatcgccc gggacgcggt ggcggaggtc ttcgagaggc tcggagcgcg agaccggctc
     2341 gagttcttcg cgatgccgag gatggagttt tcatcgactt tggtccggga gcggatcgcc
     2401 tcgaaccggc cctggaaaca cctcgtaccg caaggggtgg cggaaatgat cgacaatgag
     2461 gatctgtatg gcagcaagca gtgaagaact gagccgggat atcgcccggc tgctggatca
     2521 aaagctcggc gaggacatcg tctgtctcga catgcgcgag ctggtcacct acaccgacta
     2581 cctggtgatc tgcaccgccc gcaacgaacg gcaggccgag gcgattgccg acgaggtcca
     2641 cctgcgcctc aagcacgaca actcgctgct gccggtcaat ccggaccgct ccggtgacgt
     2701 cggatggacc gtgctcgact acctcgactg cgtcctccac atcttcaccg aggaagccag
     2761 gcagcgctac gacctcgaag acctctggca cgaagcgccc cacctcgatc tcggtctcga
     2821 ccagtccggg gcgaagtccg cctcggccgc ctgatcccgg taa
//
LOCUS       DAOQFI010000180         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17928_length_2862_cov_3.205558, whole genome shotgun sequence.
ACCESSION   DAOQFI010000180 DAOQFI010000000
VERSION     DAOQFI010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17928_length_2862_cov_3.205558"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..388
                     /locus_tag="PLE93_04125"
     CDS             116..388
                     /locus_tag="PLE93_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNH86267.1"
                     /translation="MRPTKTTHFHLKDAFGRPGIDGEDFIFCMLGEGKVPWPETFAAL
                     DRVGYEGPLSVEFEAYAYYEQVLGSDPAAAARLARGQVAALLGEGS"
     gene            385..1386
                     /locus_tag="PLE93_04130"
     CDS             385..1386
                     /locus_tag="PLE93_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNH86268.1"
                     /translation="MKVALCGLGEIGQIHLRAIRESGHELVGVLELNRRLAVESVGDE
                     VPIHTEVMEMVELTEPDVVDICLPHHLHVPIAVAALEAGADVLLEKPMAIDLAGCDEI
                     IAAAEAFDHRVGISHNQIHFAPHRRLRELIDAGRLGQIRAIYERLWMGGKYGGWREDS
                     SQVGGGLLMDAGVHRVYMAEYLGGPVSTVSAVMDKPRSEDSFVLTLEFESGATGVIQG
                     GYHGPEGVFDDRIEIQGSAGMAEALGCEAFFEGDLSGDLRLRARLDGAWVDDPVTGDW
                     DESVIASVRACLDDLAAGREPESGLAAGRSAVAVIEAAYRSAETGNSIRVSDLDGRQ"
     gene            1386..2126
                     /locus_tag="PLE93_04135"
     CDS             1386..2126
                     /locus_tag="PLE93_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNH86269.1"
                     /translation="MKSIDIGNEEKGSLREIKAESSLTARVRESIREAIIDGSLEPGS
                     LHSVKSLADIFKVSRTPVREALIDLAGADMVEFERNRGVRILETSVHDLEEILVLRIL
                     LEVPATYRAAEHIDADGLSALRQELDAMVEAAENGDEPTMMQHDRRFHELINTASGNS
                     RLTEYVDSLRDLILTRGVSTVDNTRSLTEIVDEHEGILTAIASGDGEAAAAAMKEHLV
                     NTASLLLQQEGGRSSSLSLGWERLVAGG"
     gene            2123..>2862
                     /locus_tag="PLE93_04140"
     CDS             2123..>2862
                     /locus_tag="PLE93_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86270.1"
                     /translation="MSKLVKLAVASWRRQKLRTAMTVGSLALSVGAVVFVYSLSVAFE
                     SSGSQAIESAIGKADIWVVPAKGVEVNRKDESIDAIGSLPSGIEAEVRSLPAVTAVTA
                     VTGSGAGTLQVTTSNPKLTADAMKSKGLTVTSDPARSTATGDAPALAYLITASEDRFG
                     VYTFSQQFEAVQVNEVASSVLGVVGRVTLALGFLAVLTSLLISIDERRREFGILAAVG
                     ITDDVLYLFLVESGLLIAMGLVVGVIFGG"
BASE COUNT          472 a          924 c          989 g          477 t
ORIGIN      
        1 gtcggccatc tcggcagctc ttcggtccgc tacgagatcg gcctcttcgc cggggacggc
       61 gaaccgctac cgaaccacgg ccgcaaatgc ctcggtgaat tccttccggc cccctatgcg
      121 accgacgaaa acgacgcact tccacctcaa ggacgccttc ggccggcccg gcatcgacgg
      181 cgaggacttc atcttctgca tgctcggtga gggcaaggtt ccctggccgg agactttcgc
      241 ggcgctcgac cgagtcggat acgaaggccc gctctcggtc gagttcgagg cctacgctta
      301 ttacgagcag gtgctgggca gcgacccggc tgccgcggcc aggctcgccc gcggccaggt
      361 ggcggccctg ctcggggagg gctcgtgaag gtcgcgctct gcggactcgg cgagatcggc
      421 cagatccacc tcagggcgat ccgcgaaagc ggtcacgaac tggtcggtgt gctggaactg
      481 aaccgccgcc tcgcggtcga aagcgtcggt gacgaggtgc cgatccacac cgaggtcatg
      541 gagatggtcg aactgaccga gccggatgtg gtcgacatct gcctgcctca ccaccttcac
      601 gtgccgatcg ccgtggccgc gctcgaagcc ggcgccgacg tgctgctcga gaagccgatg
      661 gcgattgatc tcgccggctg tgacgagatc atcgccgcgg ccgaagcctt tgaccaccgg
      721 gtcgggatct cccacaacca gatccacttc gcgcctcacc ggcgtctgcg tgagctgatc
      781 gacgcgggcc ggctggggca gatcagggcg atctacgagc gcctgtggat gggcggaaag
      841 tacggcggct ggcgggagga ttcatcccag gttggcggcg ggctcctgat ggacgcgggg
      901 gtccaccggg tctacatggc cgagtatctc ggcggcccgg taagcaccgt ttcggcggtg
      961 atggacaagc cccggtcgga ggacagcttc gtcctcaccc tcgagttcga gagcggagcc
     1021 accggcgtga tccagggtgg ctatcacggg cccgaagggg tcttcgacga ccggatcgag
     1081 atccagggct cggccgggat ggccgaggcg ctgggctgcg aggcgttctt cgaaggtgac
     1141 ctttccgggg acctcaggct gcgggcccgg ctcgacggag cctgggtcga tgacccggtg
     1201 accggcgact gggacgagtc ggtgatcgcc tcggtccggg cctgcctcga cgatctcgcc
     1261 gcgggccggg aacccgagtc ggggctggcg gcgggccgct ccgcagttgc cgtaatcgag
     1321 gctgcctatc gttcggcaga gaccggcaac tcgattcgcg tcagcgatct ggatgggagg
     1381 cagtgatgaa gagcatcgac atcgggaacg aggaaaaggg atcgctgcgg gaaatcaagg
     1441 ctgaatcgag cctcacggcg agggtgcgcg agtcgattcg cgaggcgatc atcgacggta
     1501 gcctggagcc cgggtcgctt cactcggtca agtcgctggc tgacatcttc aaggtttccc
     1561 ggaccccggt tcgcgaggcc ctgatcgacc tcgccggggc cgacatggtc gagttcgagc
     1621 gcaatcgcgg tgtccggatt ctcgagacct cggtccacga cctcgaagag atcctcgtcc
     1681 tgcggatcct actcgaggtt cccgccacct accgggcggc cgagcacatc gatgccgacg
     1741 ggctcagcgc cctcaggcag gagctcgacg cgatggtcga ggcggccgag aacggggacg
     1801 agccgaccat gatgcagcac gaccgccgct tccacgaact gatcaacacg gcctccggca
     1861 actcccggtt gaccgaatac gtcgactcgc ttcgcgacct gatcctgacc cgcggagttt
     1921 cgaccgttga caacacgcgc tcgctgacgg agatcgtcga cgagcacgaa gggatcctca
     1981 ccgcgattgc ttccggcgat ggtgaagcgg cagcggccgc gatgaaggag cacctggtca
     2041 acacggcctc gctcctgctt cagcaggagg gcggacgcag ctcgtcgctg agcctgggct
     2101 gggagaggct ggtcgccggc ggatgagcaa gctggtcaag ctcgcggtcg ccagctggcg
     2161 acgccagaag ctccgcaccg cgatgacggt cggctcgctg gccctttcgg tcggggcggt
     2221 cgtcttcgtc tattcgcttt cggtcgcttt cgagagttcc ggctcccagg cgatcgagtc
     2281 ggcgatcggc aaggccgaca tctgggtggt gccggccaag ggggtcgagg tcaaccggaa
     2341 ggacgagagc atcgacgcga tcggcagcct gccgtcgggg atcgaggccg aagtcagatc
     2401 gctgccggca gtcactgccg tgaccgcggt caccggcagc ggtgccggaa cccttcaggt
     2461 caccacttcc aacccgaagc tgaccgcgga cgcgatgaag agcaagggcc tgaccgtgac
     2521 ttcggacccg gctcgcagca cggcgaccgg agacgccccg gccctcgcct acctgatcac
     2581 ggccagcgag gaccgcttcg gcgtctacac cttctcccag cagttcgagg cggttcaggt
     2641 caacgaggtg gcctccagcg tgctcggggt ggtcggccgg gtcacccttg cgctcggttt
     2701 cctcgcggtc ctgacctcac tgttgatctc gatcgacgaa cgccgccgcg agttcggcat
     2761 cctcgccgcg gtagggatca ccgatgacgt gctctacctg ttcctggtcg aatccggact
     2821 cctgatcgcg atggggctgg tggtcggggt gatcttcggc gg
//
LOCUS       DAOQFI010000181         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_17955_length_2860_cov_3.607843, whole genome shotgun sequence.
ACCESSION   DAOQFI010000181 DAOQFI010000000
VERSION     DAOQFI010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17955_length_2860_cov_3.607843"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PLE93_04145"
     CDS             complement(<1..1531)
                     /locus_tag="PLE93_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86271.1"
                     /translation="MSFADFIAGLVHLALVAGFTGFTAFRLRARLLPGWGGAPARLVE
                     AVAGVGLLVLAAELLGLFGWLEILPLEVLLGLIAVSAWLRLVPRRPGTGDSVPPSPAV
                     STGAQVLAFLAAFAVFAQWGAFTSYNLDHGITNFDSVWYHMPFAAEIARTGSVTGFFH
                     PETVFLNWFYPQNSELVHAVAIVLTGRDFVSIFINLGWLALGLLAGWCVGRPYGRPQL
                     TMLGVAVLMATHTLVVREPGTGKNDVMAAALILASVAIMLNRSTSRPDPPGRVSPDWA
                     MAAGGLAIGLAAGTKVTALAPAFLVTCAVIYATIPGRRLRASGVWLGAAFIGGGWWYL
                     RNLFAAGNPMPQITRIGPLDLPAPDRLQVGRSDFSVFHYLTDTAVWKDYFVPGLDQGF
                     GHVWPLLFALVVGGLFLVMFTAHGRLTRTHGAVALLAILAYLVTPLSAAGPEGDPSAF
                     AINLRFLIPALGLAVVLIPLSSWFDRGWRRLALGLILIGLFIGTSATDPVISAPGRNF
                     GV"
     gene            complement(1557..1934)
                     /locus_tag="PLE93_04150"
     CDS             complement(1557..1934)
                     /locus_tag="PLE93_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86272.1"
                     /translation="MSVRAHTYGDARRPPRRRPAPRRNAGKPGAGRIQWDRFGRIVLA
                     IVIVLLFAAMIKPLWNAVHTYRLAGQTKVRLHQAQAEQAMLQRQLRRASSESTLQREA
                     RRLGMIRPEESAWVVNGTKGDSR"
     gene            complement(1968..>2860)
                     /gene="eno"
                     /locus_tag="PLE93_04155"
     CDS             complement(1968..>2860)
                     /gene="eno"
                     /locus_tag="PLE93_04155"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNH86273.1"
                     /translation="DLYGESDPSLLPVPMMNILNGGAHADNSVDFQEFMVMPAGTSSF
                     SECLQVGTEVFHSLKKILSGHGLATAVGDEGGFAPDLSSNEAALEMLIEGIEAAGYKP
                     GEEVFIALDPATSEIYSDGVYRLEHEDRDLSPQQMADYWQSAAERFPIVSIEDGMAED
                     DWDGWKLLTEKLGDSVQLVGDDLFVTNPARLKRGIDLGVGNSILVKVNQIGTLTETLE
                     AIRMAADAGYSAVISHRSGETEDTTIADLAVATGAGQIKTGAPSRSDRVAKYNRLLRI
                     EEDLGERARFGGVSVLKRGN"
BASE COUNT          506 a          954 c         1005 g          395 t
ORIGIN      
        1 ccaccccgaa gttgcggccc ggagccgaga tcaccgggtc ggtggcgctg gtgccaatga
       61 agagcccgat caggatcagg cccagggcaa ggcggcgcca gccgcggtca aaccacgagg
      121 aaagcgggat caggaccacc gccaggccaa gagccggaat caggaagcgc aggttgatcg
      181 cgaaggcgct gggatcgcct tccggtccag cggcgctcag cggcgtgacc aggtaggcca
      241 ggatggccag cagggcgacc gcgccgtggg tgcgggtcag acgcccgtgg gcggtgaaca
      301 tgaccaggaa caggccgccc acgaccaggg caaagaggag cggccacacg tggccaaagc
      361 cctgatcgag accgggcacg aagtagtcct tccagaccgc ggtgtcggtc aggtaatgaa
      421 agaccgagaa gtcggagcga ccgacctgga ggcggtcggg agccggcagg tcgagcgggc
      481 cgatcctggt gatctgcggc atcgggttgc cggccgcgaa caggtttctc aggtaccacc
      541 agccgccgcc aatgaaggcg gcgccgagcc agactccgct ggcccgcaga cgccggcccg
      601 ggatcgtcgc atagatcacc gcgcaggtga ccaggaaggc cggggccagg gccgtcacct
      661 tggttcccgc ggcgaggccg atcgccagtc cccccgccgc catcgcccag tccggcgaga
      721 cccgccccgg cggatccgga cggctggtcg agcggttcag catgatcgcg acggaggcga
      781 gaatcagggc ggccgccata acgtcgttct tgccggtgcc cggctcccgc acgaccaggg
      841 tgtgggtggc catcagcacg gccaccccga gcatggtcag ctgcggccgg ccgtaggggc
      901 ggccgacgca ccagccggcc agcagcccaa gcgccagcca gccgaggttg atgaagatcg
      961 agacgaagtc gcgaccagtc aggacgatcg cgaccgcgtg gacgagttcc gagttctggg
     1021 ggtagaacca gttgaggaag acggtctccg gatggaagaa gccggtcacc gacccggtgc
     1081 gggcgatctc ggccgcgaac ggcatgtggt accagaccga atcgaagttg gtgatgccgt
     1141 ggtcgaggtt gtaggaggtg aaggcgcccc actgggcgaa gacggcgaag gccgccagga
     1201 aggcgaggac ctgggctccg gtggaaaccg ccggggatgg cgggaccgag tcaccggtcc
     1261 cgggccgccg cggaacgagc cggagccatg ccgacaccgc gatcagtccg agcaggacct
     1321 ccaggggcag gatttccagc cagccgaaga ggccgagcag ctcggcggcc aggaccagca
     1381 ggccgacccc ggccaccgcc tcgaccagac gggcaggtgc gccgccccac ccgggcagca
     1441 gacgggccct gagccggaac gcggtgaaac cggtgaagcc cgccaccagg gccaggtgaa
     1501 cgagcccggc gatgaagtca gcgaaactca attgaggcca ccggcctcgc cactccctac
     1561 cgggagtcgc ccttggtgcc gttcacgacc caggccgact cctccggcct gatcatcccc
     1621 agtcgccgcg cctcgcgctg gagcgtggac tcgctgctgg cccgccggag ctgacgctgc
     1681 agcatcgcct gctcggcctg ggcctggtgc agccgcacct tggtttgccc ggccagccgg
     1741 taggtgtgaa cggcgttcca cagtggcttg atcatcgcgg cgaacaggag aacgatcacg
     1801 atcgccagca cgatgcggcc gaagcggtcc cactggatcc ggcccgcgcc cggcttgccg
     1861 gcgttccggc gaggggcagg ccggcggcgt ggcggccgcc gggcatcgcc ataggtatgc
     1921 gcgcgcaccg acacgtccga cccttctagg ccggacggtc aattccttca attccctcgc
     1981 ttcagaaccg atacgccgcc gaaacgggcc cgttcgccca ggtcttcctc aatccggagc
     2041 aggcggttgt acttggcgac ccggtccgac cgggagggcg ccccggtctt gatctggccg
     2101 gcccccgtcg ccacggcgag atcggcgatc gtggtgtcct cggtctcacc ggaacggtgg
     2161 gagatcaccg cggagtagcc ggcgtcggcg gccatgcgga tcgcctccag ggtttcggtc
     2221 agggtgccga tctggttgac cttgaccagg atcgagttgc cgacgcccag gtcgattccg
     2281 cgcttcaaac gggccgggtt ggtcacgaac aggtcatcgc cgaccagctg gaccgaatcg
     2341 ccgagctttt cggtcagcag cttccagccg tcccagtcgt cctcggccat gccgtcctcg
     2401 atcgagacga tcgggaagcg ctccgctgcc gactgccagt aatcggccat ctgctgcggg
     2461 ctgagatcgc ggtcttcgtg ttcgagccgg tagacgccat ccgagtagat ctcggaggtg
     2521 gccgggtcga gcgcgatgaa gacctcttcg ccgggcttgt aaccggctgc ctcgatgcct
     2581 tcgatcagca tctcgagcgc cgcctcgttc gaggagaggt ccggggcgaa gccgccttcg
     2641 tcaccgaccg ccgtggccag cccgtgcccg gagaggatct tcttcagcga gtggaacacc
     2701 tcggtcccca cctgcaggca ttcggagaag ctcgaggtgc cggccggcat gaccatgaat
     2761 tcctggaaat cgaccgaatt gtcggcgtgg gcgccgccgt tgaggatgtt catcatcggc
     2821 accggcagga gcgacgggtc cgactcgccg tagagatcgc
//
LOCUS       DAOQFI010000182         2851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18046_length_2851_cov_3.095494, whole genome shotgun sequence.
ACCESSION   DAOQFI010000182 DAOQFI010000000
VERSION     DAOQFI010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18046_length_2851_cov_3.095494"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLE93_04160"
     CDS             complement(<1..1241)
                     /locus_tag="PLE93_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNH86274.1"
                     /translation="MKKLVLAVTTAFLAICCCAPMAAAAPDRDPIPPPTDYWQDKGFI
                     LMAHQGGEWDFPPNTLFAYKSAMAIGADMLDMDAYVTRDDHIVLTHDLDARSNSNLTD
                     DMFGGRHDINDLDLVELKTLDFAYKWSPRDGSASVPFRGIATGSAQPPTGFTANDFRI
                     PTFDELLDAFPDTPINIELKQVGALTPADIQATAEKMADILEAHPGHDENVIINSFGQ
                     EMLDAMHAARPEHKSFSGSQSALLGYIQGNPITPTPVAVEPPDYYNLGSSLQPTWVRT
                     VPILSPFTEYDGYRIYVWGSDHDPFQDTDEFYAKLIEEGADAYNTPSPLTLAAYLCSA
                     GIPQADGTPRCPEQECAEGQTGYAPDHCVDIPVDPMIEIKSLKLTPKQGQVKAGKKAV
                     LTLQVSTTDGIGGGNTPIRLRS"
     gene            1338..1574
                     /locus_tag="PLE93_04165"
     CDS             1338..1574
                     /locus_tag="PLE93_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86275.1"
                     /translation="MSLDPEYVVRIAKLEQKVSELYRHLGVAEPSGNEPLSPEVQALL
                     QQNNTIEAIKVHRDQTGLDMAAAKAAIDEYLNQG"
     gene            complement(1604..>2851)
                     /locus_tag="PLE93_04170"
     CDS             complement(1604..>2851)
                     /locus_tag="PLE93_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNH86276.1"
                     /translation="PPVEEIANACVECGFCEPVCPSRHLTTTPRQRIVLRREMVRQGE
                     DTPVRRALLQEFEYEAIETCAADGSCLLACPVGIDTGKFIKGFRAEQHSPAAEKMALR
                     AARRWDRIEKLARTGLKLGGAVSSVASDGAMRGLTRAARKAMSADLVPEWGEDMPPAA
                     QPKLPQTTSHGAAAVYMPACINRMFGRSKDDPGKSLPEAMVEVSARAGKPVWIPPDIA
                     GTCCSVPWSSKGYRSAHEFKANQTVERIWRWTGGGTLPLVIDASSCGHGLIDFGEGVL
                     SEENAARHAELKIIDSVQWAEEFLLPGLEVTNKAESVTIHPTCSGRHLGLERSLTRIA
                     DALAERVEIPNVATCCGFAGDRGLLHPELTASATAAEAAEVKSEPTAKYISSNRTCEV
                     GLERATGEPYGSFIYLLEEATRP"
BASE COUNT          441 a          879 c         1010 g          521 t
ORIGIN      
        1 gacctcaacc ggattggtgt gtttccgcca ccgatgccgt cggttgtcga gacttgcagc
       61 gtcagcacgg ccttcttgcc tgccttcacc tgcccctgtt tcggagtcag tttcaggctc
      121 ttgatctcga tcatcggatc gacggggata tccacgcagt ggtccggggc gtagccggtc
      181 tggccttccg cgcattcctg ctccgggcag cgaggggtgc cgtcggcctg cgggatgccg
      241 gccgagcaga ggtaggcggc gagagtcagc ggcgagggcg tgttgtaggc gtcggcgccc
      301 tcctcgatca acttggcgta gaactcgtcc gtgtcctgga aggggtcatg gtcggaaccc
      361 cagacgtaga tcctgtagcc gtcatattcg gtgaaaggtg acaggatcgg caccgtgcgc
      421 acccatgtgg gttgaaggct tgacccgagg ttgtaatagt caggcggctc gaccgcgacc
      481 ggagtcgggg tgatcgggtt gccctggatg tagccgagca gggcgctctg gctgccgctg
      541 aatgacttgt gctcgggccg ggccgcatgc atcgcgtcga gcatctcctg gccgaaggag
      601 ttgatgatca cgttttcgtc gtggccgggg tgcgcttcga ggatgtcggc catcttctcc
      661 gcggtcgcct ggatgtcggc cggggtcagg gcgccgacct gtttcagttc gatgttgatc
      721 ggggtgtccg ggaaagcgtc gagaagctcg tcgaaggtcg ggatcctgaa gtcgttggcg
      781 gtgaaaccgg tcggcggctg ggcgctgccg gtggcgatgc cgcggaaagg caccgaggcc
      841 gagccgtcac gcggactcca cttgtaggcg aagtcgaggg tcttgagctc gacgagatcg
      901 agatcgttga tgtcgtggcg gccgccgaac atgtcgtcgg tgaggttcga gttgctgcgg
      961 gcgtcgaggt cgtgagtaag gacgatgtga tcgtcgcggg tcacgtaggc gtccatgtcg
     1021 agcatgtcgg cgccgatcgc catcgcggac ttgtaggcga acagggtgtt gggcgggaag
     1081 tcccactcgc caccctgatg ggccatcagg atgaagccct tgtcctgcca gtagtcggtc
     1141 ggcggcggga tcggatcccg gtccggcgcc gccgcagcca tcggggcgca gcagcagatc
     1201 gccagaaaag cggttgtgac ggccaggacc agcttcttca tcatcgattc ccctgtttcg
     1261 tttctcccac gccagagtgt agtgcccgca tccggaaaat tgtcacggaa aggtgaccgg
     1321 ggagttaggg tcggagcatg agtctggatc ctgaatacgt cgtccgcata gccaagctcg
     1381 agcagaaggt gagtgagctc taccggcatc tgggggttgc cgagccgtcc ggcaacgagc
     1441 cgctctcacc cgaggttcag gctctgctcc agcagaacaa cacgatcgag gcgatcaagg
     1501 tccaccgcga ccagaccggc ctcgatatgg ccgccgccaa agccgcaatc gacgagtacc
     1561 tgaaccaggg ctagctcctc cgggcctgac cccggggtcc cggtcagggc cgggtggctt
     1621 cttcgaggag gtagatgaag gagccgtagg gttcgccggt cgcgcgttcg aggccgacct
     1681 cgcaggtccg gttggaggag atgtacttcg cggtcggttc cgacttgacc tcggccgcct
     1741 cggcggcggt ggcgcttgcg gtcagttccg gatggagcag gccgcggtca ccggcgaagc
     1801 cgcagcaggt ggcgacgttc gggatttcaa cccgctccgc gagggcgtcg gcgatccggg
     1861 tcagggacct ttccagaccc aggtggcggc ccgagcaggt cggatggatc gtgaccgatt
     1921 cggccttgtt ggttacttcg aggcccggta gcaggaactc ttcggcccat tggacggagt
     1981 cgatgatctt cagctcggcg tggcgggcgg cgttctcctc gctcagcacc ccctcgccga
     2041 aatcgatcag gccgtggccg caggacgagg cgtcgatcac cagcggcagg gttcccccgc
     2101 cggtccagcg ccagatccgc tcgaccgtct ggttcgcctt gaactcgtgg gcgctcctgt
     2161 agcccttcga gctccacgga accgaacagc aggtgccggc gatgtccggc gggatccaga
     2221 ccggcttgcc ggcccgggcc gagacctcga ccattgcctc gggcaggctc ttccccgggt
     2281 catccttgga gcggccgaac atgcggttga tgcaggccgg catgtacacg gcggcggcgc
     2341 catgggaggt cgtctgtggc agcttcggct gggcggccgg cggcatgtcc tcgccccact
     2401 cgggaaccag gtccgcgctc atcgccttgc gcgcggcgcg ggtcagaccc cgcatcgccc
     2461 cgtccgaagc gacgctggaa acggcgcccc cgagcttgag gccggtacgg gccagcttct
     2521 cgatccggtc ccagcgacgg gcggcccgca gggccatctt ctcggccgcg ggactgtgct
     2581 gctcggcccg gaaacccttg atgaacttgc cggtgtcgat cccgaccggg caggccagca
     2641 ggcaggaacc gtccgcggcg caggtctcga tcgcctcgta ctcgaactcc tggagcaggg
     2701 cccggcggac cggggtgtcc tcgccctggc ggaccatctc ccggcgcaga acgatgcgtt
     2761 gccgcggggt ggtggtcagg tggcggctgg ggcagaccgg ctcgcagaaa ccacattcga
     2821 cgcaggcgtt ggcaatctcc tcgactggcg g
//
LOCUS       DAOQFI010000183         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18056_length_2850_cov_3.428980, whole genome shotgun sequence.
ACCESSION   DAOQFI010000183 DAOQFI010000000
VERSION     DAOQFI010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18056_length_2850_cov_3.428980"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLE93_04175"
     CDS             complement(<1..723)
                     /locus_tag="PLE93_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNH86277.1"
                     /translation="MSDAPELKPNSYRDTWCGQVLGDKVGGQVRLSGWVHRRRDHGGL
                     IFIDLRDRTGLVQLVFNPDEAGGAHELGHTLRSEYVISVAGEVVTRSDETVNPELPTG
                     EFEVRVKEVQVLAEAETPPFEIEGFHGEVGEEARLKHRYLDLRRDQMQQAIKLRHRLT
                     AAMRSFLDAEGFLDIETPILTLSTPEGARDFLVPSRLQRGSFYALPQSPQLFKQLLMM
                     SGFERYFQIARCFRDEDLRNDRQ"
     gene            complement(739..945)
                     /locus_tag="PLE93_04180"
     CDS             complement(739..945)
                     /locus_tag="PLE93_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86278.1"
                     /translation="MKRTIKPGKTAAVKVTVKPAFRELVDAMTYVDARKLSVAHTVKS
                     KKVGKRTAKKPARPSLSLKVRIKN"
     gene            932..1240
                     /locus_tag="PLE93_04185"
     CDS             932..1240
                     /locus_tag="PLE93_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH86279.1"
                     /translation="MVRFTALLIAAPAFDFFAWTATAPRSTFPELTDREREILALVAE
                     GRSNEQIANRLTLSLKTIRNHVSNIFNKLQVPDRAAAIAKAREAGLGVQPLEASDPPT
                     "
     gene            complement(1233..2663)
                     /locus_tag="PLE93_04190"
     CDS             complement(1233..2663)
                     /locus_tag="PLE93_04190"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HNH86280.1"
                     /translation="MKGFLAGAFALLALLVAPAAANAVIITVNTTADEYGTGTSCSLR
                     EAITAAQNNVSFGGCPAGFASDTVSIPDGTYKITRAGTGEDGNATGDFDITGSNALDI
                     QPDASNAHVVVDGNGIDRVFQDLSSGLVKFLAIQITGGKLTGFNDGAGIQNSVGVTSL
                     ENVTVNDNSSEAQGGGVAVYNNVQVINSTIAQNNADGNGGGFYVPGGASLVARSTTVF
                     GNQADADGDGNGYGGGFAETGGLNVSFTNVLNAGNSGTALLPTDDAYDCYSGPNFYPR
                     YTLSGQPMGPLDCLVGFNPGTNMVSSDVKLDPFLRDNGGQTPTLALLPGSPAIGAGGT
                     ASPDECPGFDQNDRPRPAGKCDIGAVQFVAEPGLIITKLLPKKKVIKRKKTRAITVQV
                     RNDGTGPAKQVKICLKLNRAAKKGLKVKGKACKSVGTVAVGARKSAKIKLMAKPKARK
                     KAYSLKATFKGSNAPLATRAFKVRVK"
BASE COUNT          446 a          907 c          928 g          569 t
ORIGIN      
        1 ctgacgatcg ttgcgcaggt cctcgtcgcg gaaacagcgg gcgatctgga agtaccgctc
       61 gaagccggac atcatcagaa gctgcttgaa gagctgcggt gactgaggca gggcgtaaaa
      121 cgagccgcgc tggaggcgcg atggcacgag gaagtcgcgg gcaccttcgg gagtggagag
      181 ggtgaggatc ggggtttcga tgtcgaggaa tccctcggcg tcgaggaagg agcgcatcgc
      241 ggcggtgagc cggtggcgca gcttgatcgc ctgctgcatc tggtcgcggc gcaagtccag
      301 gtagcgatgt ttcagccggg cctcttcacc gacctcgccg tgaaagcctt cgatctcgaa
      361 gggcggggtc tcggcctcgg ccagcacttg aacctctttg acccgcacct cgaactcccc
      421 ggtcggcagt tcagggttga ccgtctcatc cgagcgggtc accacttcac cggcgacgct
      481 gatcacgtat tcggaacgca gggtgtgacc gagctcgtgc gctcccccgg cctcatccgg
      541 gttgaagacg agctggacca ggccggtccg gtcgcgcagg tcgatgaaga tcaggccacc
      601 gtggtcacga cggcggtgga cccagccgga aaggcgcacc tggcctccga ccttgtcccc
      661 caggacctgg ccacaccagg tatctcgata cgaattcggt ttcagctccg gcgcgtcgct
      721 catcgaagcg ccagtctttc agttcttgat ccgtaccttc agtgagaggc tcggtctggc
      781 cggcttcttt gccgtgcgtt tgcccacctt cttcgacttg acggtgtggg cgaccgaaag
      841 tttgcgggcg tccacgtagg tcatcgcgtc gaccagctcc cggaacgcgg gcttgaccgt
      901 caccttcacc gcggccgtct tgcctggttt gatggtccgc ttcaccgcat tgctgattgc
      961 cgcgccggcc ttcgacttct tcgcctggac cgcgacggcc ccgcgatcga cttttcccga
     1021 gctgaccgat cgcgagcggg agatccttgc gctcgtggcc gaaggacggt caaacgagca
     1081 aatcgcgaat cggctcaccc tcagcctgaa gacgatccgg aatcacgtca gcaacatctt
     1141 caacaagctg caggtgcccg accgggccgc cgcgatcgcg aaagcccgcg aggcggggct
     1201 cggggttcag ccgctcgaag cttctgaccc tcctacttga cccgcacctt gaatgcccgc
     1261 gtggcgagcg gggcgttaga gcccttgaag gtggccttga gcgagtaagc cttctttctc
     1321 gccttcggct tggccataag cttgatcttc gccgacttcc tcgcgccaac cgcaacggtg
     1381 ccgaccgact tgcaagcctt gcccttgacc ttcagcccct tcttcgcggc ccggttcagc
     1441 ttcaggcaga tcttcacctg cttggccggt ccggttccgt cattgcggac ctgaaccgtg
     1501 atcgccctgg tcttcttccg cttgatcacc ttcttcttcg gcagcagctt cgtgatgatc
     1561 agaccgggct cggccacgaa ctggaccgcg ccgatgtcgc acttgccggc cggacggggc
     1621 cggtcgttct gatcaaagcc ggggcattca tcgggactgg cggtacctcc ggcaccgatc
     1681 gcgggcgatc ccggcagcag ggcgagggtc ggggtctgac cgccgttgtc ccgaaggaag
     1741 gggtccagct tcacgtctga gctgaccatg ttggtgcccg ggttgaatcc aaccaggcag
     1801 tcgagcgggc ccatcggctg cccggaaagc gtgtagcgcg ggtagaagtt cgggccgctg
     1861 tagcagtcat aggcgtcgtc ggtcgggagg agggccgtgc cggagttgcc cgcattcaga
     1921 acattggtga agctgacgtt gagcccgccg gtttcggcga agcctccgcc gtagccgttg
     1981 ccgtcaccgt cagcgtcggc ctggttgccg aagaccgtgg tgctccgggc caccagcgag
     2041 gcgccgcccg gaacgtagaa gccgccgccg ttgccgtcgg cattgttctg ggcgatcgtg
     2101 ctgttgatga cctgaacgtt gttgtagacg gcgacgccgc cgccctgggc ttcagacgag
     2161 ttgtcgttca cggtcacgtt ctcgagcgag gtcactccca ccgagttctg gatgcctgca
     2221 ccgtcgttga aaccggtcag cttgccacct gtgatttgga tcgcgaggaa cttgaccagg
     2281 ccgctggaga ggtcctggaa gacgcggtcg atcccgttgc cgtcaaccac cacgtgcgca
     2341 ttcgatgcgt caggttgaat gtcgagggca ttgcttccgg tgatgtcgaa gtctccggtc
     2401 gcgttgccat cctcgcccgt tcccgcccgg gtgatcttgt aggtgccatc ggggatgctg
     2461 accgtgtcgg aggcgaagcc agccgggcag ccaccgaagg aaacgttgtt ctgcgcggcg
     2521 gtgattgcct cccgcagcga gcagctggtg cccgttccgt actcgtcggc ggtcgtgttg
     2581 accgtgatga tcaccgcgtt cgcggcagcc ggcgccacca gcagtgcgag aagggcgaaa
     2641 gcccctgcca gaaatccctt caccgtttcc tccagtcgtt cgggcctccc cggttccggc
     2701 cctttggatt gatcgtatgc gggtcgcctg ggcaggtcaa gccgtccggc cggctcaggt
     2761 tctgacccgg accttgtagg tgctgatgac cgtggccggg cggcgtggtg ccaggcggtt
     2821 gccgatccgc ttcgagcgga tcttctggcg
//
LOCUS       DAOQFI010000184         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18059_length_2850_cov_2.621109, whole genome shotgun sequence.
ACCESSION   DAOQFI010000184 DAOQFI010000000
VERSION     DAOQFI010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18059_length_2850_cov_2.621109"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLE93_04195"
     CDS             complement(<1..823)
                     /locus_tag="PLE93_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH86281.1"
                     /translation="MQTSETVTEFDGGPAVEPEFKNLYEAFQATVARKGDGLAIKSEA
                     EEVELSWNDLDKRVRAIAGGLHALGIQRGDRVGMLLGARSDFIPIDLAVVSIGGLPFS
                     IYQTLAPDQIEYVVEDSGTELIITEEAYLPNLLEARKNLPNLKRVVMLDGEGGDMTMA
                     ELEAMDPEFDPAPLAAEVGLDDPLTLIYTSGTTGPPKGVLLTQGNLMSMLTTVMHSVL
                     EIPESGRVISWLPAAHIAERGANYYTPVMHGLEVHVCPDPKRIIEFLPKVRPSWFF"
     gene            complement(1002..>1209)
                     /locus_tag="PLE93_04200"
     CDS             complement(1002..>1209)
                     /locus_tag="PLE93_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNH86282.1"
                     /translation="GIINLFPFLPLDGGHIFWSLVEKVRGRPVPFAVMERAGLIGFAL
                     VIGLFILGLNNDIGRLTGEGFNVR"
     assembly_gap    1210..1219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1220..2081)
                     /locus_tag="PLE93_04205"
     CDS             complement(<1220..2081)
                     /locus_tag="PLE93_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNH86283.1"
                     /translation="MSFLVAFLAFSALIVLHEAGHFTAAKAVGMRVERFFLFFPPKLV
                     SIKRGETEYGIGAIPLGGFVRITGMNPDEELPPEIAARGYYNQPVWKRIVVIGAGPAV
                     NIVLAFLILWVLAFGIQQPSQNVEQIQPGSPAAAKLQAGDRIISVDGVSGSQEKIADE
                     IAAHQCPGKQTDGCKAAEPATVVVMRDGQEKTLRITPVYDASVQRSRLGFAFGLEDAN
                     PGPVEAARISADYMWFVTSSTVSALSRLYEKEQRDQLSGVVGSYEVTRQAVDVGAREA
                     LTLIALISLSL"
     gene            complement(2111..>2850)
                     /locus_tag="PLE93_04210"
     CDS             complement(2111..>2850)
                     /locus_tag="PLE93_04210"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNH86284.1"
                     /translation="AAGAKLLPVDSEHSALFQLVENEDRAAIDRIVLTASGGPFRGCT
                     DLAGITVEQALDHPTWAMGGRITIDSATLMNKGFEMIEAHHLFDLPYEQIAVVVHPQS
                     IVHSLIDLRDGATLAHLGYPDMRVPIAYALTWPERAEVPVERLDLAKVGSLDFEAPDP
                     DTFRCLALAREAGESGGIAPCVLNAADEVAVAAFLEGKVSFIDIARVIEQTLDDIGSG
                     PAKGFEELFAVDEASRERASEIISGLN"
BASE COUNT          509 a          906 c          936 g          489 t
ORIGIN      
        1 caaagaacca ggagggccgc accttgggca ggaactcgat gatccgcttc gggtccgggc
       61 agacgtggac ctcgagaccg tgcatgaccg gcgtgtagta gttggcgccc cgttcggcga
      121 tgtgggcggc cggcagccag gaaatcaccc ggccgctttc ggggatctcg agcaccgaat
      181 gcatgaccgt ggtcagcatc gacatcaggt tgccctgggt cagcagcaca cccttgggcg
      241 gaccggtggt cccggaggtg tagatcagag tgagcgggtc gtcgagcccg acctcggccg
      301 ccagcggggc cggatcgaat tcgggatcca tggcttccag ttccgccatg gtcatgtcgc
      361 caccttcacc gtcgagcatc acgacgcgct tcaggttcgg caggttcttg cgggcctcga
      421 gcaggttggg caggtaggcc tcctcggtga tgatcagctc ggtcccggaa tcctcgacca
      481 cgtattcgat ctggtcgggg gcgagggtct ggtagatcga gaagggcagg ccgccgatcg
      541 aaaccaccgc gagatcgatc ggaatgaagt cggaccttgc tcccagcagc attccgaccc
      601 ggtcgccgcg ctggatgccg agcgcgtgga gaccgccggc gatcgcgcga acccgcttgt
      661 caaggtcgtt ccagctgagt tcaacctctt cggcctcgga cttgatggcc agcccgtcac
      721 ccttgcgggc gacggtcgcc tgaaaggcct cgtagaggtt cttgaattct ggttcaaccg
      781 cggggccacc gtcaaattcg gtcaccgtct cggacgtctg cacctgctcc tccttcgctc
      841 tcttcccaaa aagctacgcc gggaatgtat cccaaccaga gcaacgggca tataggggca
      901 accccctagg ccaaccaacc aacggagctc gcccatctat ccgttgcctc cgtgggcgag
      961 cgggttctac atggagggcg ggtccgaccc cgcccgactc cttatctaac gttgaacccc
     1021 tcgcccgtca ggcggccgat gtcgttgttg aggccgagga tgaagaggcc gatgaccagg
     1081 gcgaagccga tcaggccggc tctttccatg accgcgaacg ggaccggcct cccccttacc
     1141 ttctcgacca ggctccagaa gatgtggccg ccgtcgagcg gcaggaacgg gaagagattg
     1201 atgatgccgn nnnnnnnnnc gagcgagagg ctgatcaggg cgatcagggt cagcgcctcg
     1261 cgggccccga cgtcgacggc ctgcctggtc acttcgtagc tgccgaccac cccggaaagc
     1321 tggtctcgct gctccttctc gtaaaggcgg gacagcgccg aaaccgtgct ggaggttacg
     1381 aaccacatgt agtcggccga gattcgcgcc gcctcgaccg ggccggggtt ggcatcttcg
     1441 agaccgaagg cgaagccgag acgactgcgc tgaaccgagg cgtcatacac cggtgtgatc
     1501 cggagcgtct tctcctggcc gtcgcgcatg accacgacgg tggccggctc cgccgccttg
     1561 cagccgtcgg tctgcttgcc gggacactgg tgggcggcga tctcgtcggc gatcttctcc
     1621 tggcttccgc tcaccccgtc aaccgagatg atccggtccc cggcctggag cttcgccgcg
     1681 gccgggctgc cgggctggat ctgttcgacg ttctggctcg gttgctggat gccgaaggca
     1741 agcacccaga ggatcaggaa ggcgagcacg atgttgaccg ccggcccggc cccgatcacc
     1801 acgatccgct tccagaccgg ttggttgtaa tagccgcggg cggcgatttc cgggggcagt
     1861 tcctcgtccg ggttcatgcc ggtgatccgc acgaaaccac cgagcgggat cgccccgatc
     1921 ccgtattccg tctccccccg cttgatcgaa accagcttcg gcgggaagaa gaggaagaaa
     1981 cgctcaaccc gcattcccac cgccttggcg gcggtgaagt gcccggcctc gtggaggacg
     2041 atcagggcac tgaaagcgag gaatgcgaca agaaagctca tgagaagtca gggccggccg
     2101 gcgggccgat tcagttcaga ccggaaatga tctcagaagc gcgttcccgg cttgcttcgt
     2161 ccaccgcgaa aagctcttca aagcctttgg cggggccact cccgatgtca tcgagggtct
     2221 gctcgatcac cctggcgata tcgatgaagg agaccttgcc ttcgaggaac gcggccaccg
     2281 cgacctcatc ggcagcgttc aggacgcaag gggcgattcc gccggattcc cctgcttccc
     2341 gggcaagggc caggcagcgg aaggtgtccg gatcgggtgc ttcgaaatcg aggctgccca
     2401 ccttcgcgag gtcgaggcgc tcgaccggaa cctcagcccg ctccggccag gtcagggcgt
     2461 aggcgatcgg cacccgcatg tccgggtaac cgaggtgggc cagggtggcg ccgtcacgca
     2521 gatcgatcag cgaatggacg atcgactggg gatggaccac cacggcgatc tgctcgtagg
     2581 gaaggtcgaa caggtgatgg gcctcgatca tctcgaagcc cttgttcatc agggttgccg
     2641 agtcgatcgt gatccggccg cccatcgccc aggtcggatg gtcgagggcc tgctcgacgg
     2701 tgattcccgc gaggtcggtg cagccacgga agggaccgcc ggaagcggta aggacgatgc
     2761 ggtcgatcgc ggcccggtcc tcgttttcga ccagctggaa aagggcggaa tgctcggaat
     2821 cgaccggcag gagcttcgca ccggcggccg
//
LOCUS       DAOQFI010000185         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18075_length_2848_cov_2.830290, whole genome shotgun sequence.
ACCESSION   DAOQFI010000185 DAOQFI010000000
VERSION     DAOQFI010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18075_length_2848_cov_2.830290"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLE93_04215"
     CDS             complement(<1..768)
                     /locus_tag="PLE93_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNH86285.1"
                     /translation="MASALRKSPLRAAIGIALLLAGVAALLLLSSPGGDSGPSSGTTT
                     NSAGAGPFVKSESASSPFPPLARNLSAGLTNHQMAGQRIVAGFEGTTVPPVIRRRVKA
                     GLIGGVILFADNLGSDARIRHLTRALQRIRRPDGLRRYPLMIMVDQEGGLVKRLPGAP
                     NASAEQMGRRGPAFSRRQGALAGLNLKTSGFNVDLAPVLDVARPGGVIADTDRGFGST
                     VKKVKATAIPFARALQQTRVAATAKHFPGLGAARQNTD"
     gene            complement(831..1595)
                     /locus_tag="PLE93_04220"
     CDS             complement(831..1595)
                     /locus_tag="PLE93_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNH86286.1"
                     /translation="MLILLPPSEGKASPSGVSCLDLDSLAFAEYLSPKRAELIAALEK
                     LGKAPEKKGIEALGISKGQAGDIGRDAELLSSPVAPASKVYTGVLYDRLGLDSLPAAG
                     KRRAAKNLLISSALWGFLAPADRIPYYRFSMGARLPKIGGLPGYWRESLREAMEGAGH
                     DKEGNLVLDMRSGAYSAAWKPKQARLVTVRGFTEAGGRRKAISHMAKAVRGDVARIAL
                     SAKAMPKDPETLAGLLEAGGMTVELTEKTLDVIEQA"
     gene            complement(1662..2321)
                     /locus_tag="PLE93_04225"
     CDS             complement(1662..2321)
                     /locus_tag="PLE93_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH86287.1"
                     /translation="METGAESAAEKLTLVLVDDHVVVRSALKVLLESEPDLEVVAEAG
                     TADDAIRYVGAHKPDVLVLDLNMPGRPSLEAIPDIQAASPDTKIVILTMRNEPAFARQ
                     ALQAGVMGYVLKEAADTELVLAVRRAADGDTYLQPSLYQHLADKDKAEGRTGRTLSDR
                     EVEVMRLIALGHTNAEIADRLFLSVRTVESHRASIQKKLDIGSRAELVRYALDQGLIS
                     A"
     gene            2432..>2848
                     /locus_tag="PLE93_04230"
     CDS             2432..>2848
                     /locus_tag="PLE93_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH86288.1"
                     /translation="MNEATVSLDEARLRRLIEVGRSLVVESDPEAVINDALEAARELT
                     GARYAALGVLDSRRSELEQFLTRGIEAKLREQIGHPPHGGGVLGLLIEEPEPLILDDV
                     SLHPRSQGFPPGHPEMKSFLGVPVKIRGEAWGNLYLT"
BASE COUNT          466 a          949 c          970 g          463 t
ORIGIN      
        1 gtcggtgttc tggcgggccg cacccagacc cgggaagtgc ttggcggtgg cggcgaccct
       61 ggtctgctga agagcccggg cgaaggggat tgcggtcgcc ttcaccttct tcacggttga
      121 gccgaaaccg cggtcggtgt cggcgatgac tcctccggga cgggccacgt ccagcaccgg
      181 agcgaggtcg acgttgaagc ccgacgtctt gagattgagg ccggcaagag ctccctggcg
      241 gcgggagaag gcggggcccc gcctgcccat ctgctcggcc gaggcgttcg gggcccccgg
      301 cagccgtttg acgagcccgc cttcctggtc gaccatgatc atcagcgggt agcggcgcag
      361 gccgtcgggc cgccggatcc gctgaagcgc cctggtcaga tgccgaatcc gcgcgtccga
      421 gccgaggttg tcggcgaaga ggatcactcc cccgataagt ccggccttga cccggcggcg
      481 gatgaccgga gggaccgtgg tgccctcgaa cccggccacg atccgctggc cggccatctg
      541 atggttggtc agcccggcgg aaaggttgcg ggccaagggc gggaagggcg agctggcgct
      601 ctccgacttc acaaagggcc cggccccggc ggaattcgtc gtggtgcccg aagaagggcc
      661 cgagtcaccg cccggcgagg aaagcagcag gagagccgcc acaccggcca gcaaaagcgc
      721 gatgccgatc gccgcacgca ggggtgattt tctcagggcg gaagccatac atcgatgata
      781 cggatcggcc ccgaacggcc cgcctgccaa agcagaagag cgggtccgga tcaggcctgt
      841 tcgatgacgt cgagggtctt ttcggtcaac tcgaccgtca ttcccccggc ttcaagcagg
      901 ccggccaggg tttctggatc cttcggcatc gccttcgccg agagggcgat gcgggcgaca
      961 tcgccgcgga ccgccttggc catgtgggag atcgccttgc gcctgccccc ggcctcggtg
     1021 aagccccgga ccgtgaccag ccgggcctgc ttcggcttcc aggcggccga gtacgccccg
     1081 gagcgcatgt caaggacgag gtttccctcc ttgtcgtggc cggccccctc cattgcctcg
     1141 cggagcgact cccgccagta gccgggcaga ccgccgatct tcggcagccg ggcgcccatc
     1201 gagaagcggt agtaggggat ccggtcggcc ggggcgagga agccccaaag cgccgaggag
     1261 atcagcaggt tcttcgcggc ccttcgcttg ccggccgcgg gaagggaatc gaggccaagc
     1321 cggtcgtaca gcaccccggt atagaccttc gaggccggag cgaccgggga actgagtagt
     1381 tcggcgtccc gcccgatgtc cccggcctgg cccttcgaaa tgcccagcgc ctcgatgccc
     1441 ttcttctccg gggccttgcc aagcttttcc agagcagcga tcaactccgc ccgcttcggg
     1501 ctcaggtact cggcaaaggc cagtgagtcg agatcgagac aagacacgcc cgagggagaa
     1561 gccttgccct cggaaggagg tagcaggatc agcacgccgt gaatctaccg acggagcccc
     1621 tcccaaccat ccctgggcgg tgccgactcc cgtcaagcgc gttacgcgct gatcaacccc
     1681 tgatccagtg cgtaacgcac cagttcggcg cggctgccga tgtcgagctt cttctggatg
     1741 ctggcccggt ggctttccac cgtgcggacg ctgaggaaaa gccggtcggc gatctccgcg
     1801 ttggtgtggc cgagcgcgat caggcgcatt acctcgacct cgcggtcgct cagggtacgg
     1861 ccggttcggc cttcggcctt gtccttgtcg gccaggtgct ggtagaggga aggctggaga
     1921 taggtgtcgc cgtcggccgc gcgccggacc gcgaggacca gttcggtgtc ggcggcctcc
     1981 ttgagcacgt atcccatgac cccggcctga agggcctgcc gcgcgaaagc gggctcgttt
     2041 cgcatggtca ggatcacgat cttcgtgtcc ggggaggcgg cctggatgtc cgggatcgcc
     2101 tcgaggctgg ggcggcccgg catgttgagg tcgagcacca gtacatccgg tttgtgggca
     2161 ccgacataac ggatcgcgtc atcggcggtg ccggcctcgg ccaccacctc caggtcgggt
     2221 tcggattcga gcaggacctt gagggccgat cgcacgacga cgtgatcatc gaccagcacg
     2281 agagtcagct tctccgcagc gctttccgca cccgtttcca tcaagggaag gctacccgcc
     2341 ggggcgggtt ccatttccgt acctttccgc agacttcgat ttaccaggga ggggagcgga
     2401 atccggcagg cctgcctcta caatccccgg cgtgaacgaa gcgaccgtca gtcttgatga
     2461 ggctcgcctg cgccggttga tcgaagtcgg ccgcagcctg gtcgtcgagt ccgacccgga
     2521 agcggtgatc aacgacgcgc tggaggcggc ccgcgaactg accggggcgc gctacgcggc
     2581 actcggagtt ctcgacagcc gccgctccga actcgaacag ttcctgaccc gcggcatcga
     2641 ggcgaaactg cgggaacaga tcggccatcc tccccatggc ggcggcgtgc tcggtctgct
     2701 gatcgaggaa ccggagccgc tgattctcga cgacgtcagc ctccaccccc gatcgcaggg
     2761 gtttcctccc ggccacccgg agatgaagtc cttcctcggg gtcccggtga agatccgcgg
     2821 cgaagcctgg ggcaacctct acctgacc
//
LOCUS       DAOQFI010000186         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18125_length_2843_cov_2.956241, whole genome shotgun sequence.
ACCESSION   DAOQFI010000186 DAOQFI010000000
VERSION     DAOQFI010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18125_length_2843_cov_2.956241"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLE93_04235"
     CDS             complement(<1..972)
                     /locus_tag="PLE93_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNH86289.1"
                     /translation="MAFDKASVRDADVDGKRVLVRVDFNVPLDGEEVTDDTRIRAALP
                     TIEYLTGRGARVILCSHLGRPKGQVVPELSLAPAAKRLSELTGQPVGLADDVAGQDSE
                     AKVEDLQPGQILLLENTRFEPGETENAPALAARLAALADIYVNDAFGAAHRAHASTAG
                     VAADLPAYAGLLLEKEVENLTSVATDPARPLVVVLGGAKVSDKVGVIDRFLEIADQIL
                     IGGAMCFPFYKAQGHAIGGSLCHDDDIPLAREALEKAAAGNCELVLPTDLALGREFSA
                     DTDTEELDGVDVPEGWMGLDIGPKSAADYAARVGSAGTVLWNGPMGAF"
     gene            complement(997..1998)
                     /gene="gap"
                     /locus_tag="PLE93_04240"
     CDS             complement(997..1998)
                     /gene="gap"
                     /locus_tag="PLE93_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNH86290.1"
                     /translation="MSVKVAINGFGRIGRNVFRAAHDSGADLDIVAINDLVDTETLAH
                     LLKYDSILGRLDAEVSAGEGSITVDGKEIKVLAERDPADLPWGDLGIDVVLESTGFFT
                     KRDDAAKHLSAGAKKVIISAPATDPDVTVALGVNFDDYDPENHNIISNASCTTNCLAP
                     FAKAINDAVGIEQGLMTTIHAYTSDQRLQDMPHKDLRRARAAAQNLIPTTTGAAKAVG
                     LVLPDLAGKLNGFAVRAPVITGSVVDLTFTPSKATSAEEVNAAVKAAAEGPLKGILAY
                     TEDPIVSTDIVGDPHSSIFDAALTQVIGDGTLVKAVSWYDNEWGYSNRCVELLGKVL"
     gene            complement(2305..2667)
                     /locus_tag="PLE93_04245"
     CDS             complement(2305..2667)
                     /locus_tag="PLE93_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86291.1"
                     /translation="MIHHIVLEVSDLPRASSFYDALLSPLGWRRHVNETNAVGYGISR
                     PILFITERTGVRAEGTMVSLGAPGIAAVKAAWEGGTNSGGSNVAQPGETSAHGSGSYS
                     AFLRDPDGHDIEITVSQD"
BASE COUNT          467 a          884 c          988 g          504 t
ORIGIN      
        1 gaaggccccc atcggcccgt tccagagcac ggttccggcc gatccgaccc gggccgcgta
       61 atccgctgcg gacttcggac cgatgtcgag gcccatccat ccttccggca cgtccactcc
      121 gtcgagttcc tcggtgtcgg tgtcggccga gaactcgcgg ccgagcgcga ggtcggtcgg
      181 caggaccagc tcgcaattgc cggcggcggc cttctccagg gcttcacggg cgagcgggat
      241 gtcgtcgtca tggcagagcg agccaccgat cgcgtgcccc tgggccttgt agaaggggaa
      301 gcacatggcg ccgccgatca ggatctggtc ggcgatctca aggaagcggt cgatcactcc
      361 gaccttgtcg gaaaccttgg ccccgcccag caccacgacc aggggccggg ccgggtcggt
      421 ggcgaccgag gtcaggttct cgacttcctt ctccagcagc aggcccgcgt aggcgggcag
      481 gtctgcggcc acgccggcgg tgctggcgtg ggcccggtgg gcagcaccga aggcgtcgtt
      541 cacatagatg tcagcgagcg cggcgagccg ggccgcgaga gccggggcgt tctcggtctc
      601 gcccggttcg aagcgggtgt tttcaagcag caggatctgg cctggctgaa ggtcctcgac
      661 cttcgcttcg gagtcttggc cagcgacgtc atccgccaac ccgaccggct gcccggtcag
      721 ctccgacagt cgcttcgccg ccggcgccag cgaaagctcc ggcacgacct ggccctttgg
      781 ccggcccagg tgggaacaga gaatgaccct ggccccgcga ccggtcagat actcgatggt
      841 tggcagcgca gccctgatcc gggtgtcatc ggtgacctct tccccgtcga ggggaacgtt
      901 gaagtcgacc cggaccagga ctcgcttgcc gtcgacgtcg gcgtcgcgaa ccgacgcctt
      961 gtcgaaagcc atttccagct cctcgctgaa gccgtcctag aggaccttgc cgagcagctc
     1021 gacgcagcgg ttcgagtaac cccactcgtt gtcgtaccag gaaactgcct tgaccagggt
     1081 gccgtcaccg atcacctggg tcagggctgc gtcgaagatc gacgaatggg ggtcaccgac
     1141 gatgtcggtc gagacgatcg ggtcctcggt gtaggccagg atgcccttga gcgggccctc
     1201 ggccgctgcc ttgaccgcgg cgttgacctc ttcggcgctg gtcgccttcg agggggtgaa
     1261 ggtcaggtcg acgaccgaac cggtgatcac cggagcacga accgcgaagc cgttcagctt
     1321 gccggccagg tcgggcagga ccagtccgac ggccttggcc gcaccggtgg tggtcgggat
     1381 caggttctgc gcggcagcac gggcccggcg caggtccttg tggggcatgt cctgaagccg
     1441 ctggtccgag gtgtaggcgt gaatcgtggt catcaggccc tgctcgatgc cgaccgcgtc
     1501 attgatcgcc ttggcgaagg gggccagaca gttggtggtg caggatgcat tcgagatgat
     1561 gttgtggttc tcggggtcgt aatcgtcgaa gttgactccg agcgccacgg tcacgtcggg
     1621 atcggtcgcc ggcgccgaga tgatgacctt cttcgcgcct gccgacagat gcttggcggc
     1681 gtcgtcgcgc ttggtgaaga aaccggtcga ctcgagaacc acgtcgattc caaggtcgcc
     1741 ccagggcagg tcggccgggt cacgctcggc caggaccttg atctccttgc cgtcgacggt
     1801 gatcgagccc tcgccggcgc tcacctcggc gtcgaggcgc ccgaggatcg agtcgtactt
     1861 gagcaggtgc gcgagggtct cggtgtcgac caggtcgttg atcgcgacga tgtccagatc
     1921 agcaccggaa tcgtgcgctg cccggaaaac gttccggccg atccggccaa aaccgttgat
     1981 tgccaccttg acgctcattc gctaccgcct cctgggaaaa ctgggttggt gaaactccga
     2041 ttacaccttg gattctacgg ccccgccacc cccggtgccc tgcggtcaaa caccgaaatc
     2101 aggggtgggt cggatttccg tccctgctgc cggttatccg acccactgcc ggtgaacagc
     2161 ggcattgacg ggggaatgtg gggtcacccg tagacctgtg gcggctccga gtccggttca
     2221 ggcccgagtt tcgggaggtg cctatggatt tcgtctatat gaacgaaatc cataggcacc
     2281 tgccccggat cgaggccggg acctctagtc ctgggagacg gtgatctcta tgtcgtgacc
     2341 gtcagggtcg cggaggaagg cggagtagga gcccgatccg tgggcgctgg tttcgccggg
     2401 ctgggcgacg ttcgagccgc cggagttggt tccgccttcc caggcggcct tgacggccgc
     2461 gatgccgggg gcgccgaggc tgaccatcgt gccttcggcc cggactccgg ttcgttcggt
     2521 gatgaagagg atcggacggg aaatgccgta tccgaccgcg ttcgtttcgt tcacgtggcg
     2581 tcgccacccg agtggcgaga gcagcgcgtc atagaacgag gaggcgcgag ggagatcgga
     2641 aacttcgagg acgatgtggt gaatcactca cggcaggcta atggagatgc aacaaaacgg
     2701 gaacctgacc gttggcgagt ggtcgagggc ggccgggcgg aacggtgaaa tccggtccct
     2761 ggatttgcgg cgccgggcga ctactgccgg ggggtccagc ggaagtagcg caaggcgatg
     2821 aggaatccgg ccaggcccca gat
//
LOCUS       DAOQFI010000187         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18174_length_2839_cov_3.199713, whole genome shotgun sequence.
ACCESSION   DAOQFI010000187 DAOQFI010000000
VERSION     DAOQFI010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18174_length_2839_cov_3.199713"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            580..759
                     /locus_tag="PLE93_04250"
     CDS             580..759
                     /locus_tag="PLE93_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86292.1"
                     /translation="MDPERGIKTRHGNRAHRVHAEGQGRETDQECDADPKMLKQARVH
                     NQLQEAGDQNLDSMT"
     gene            complement(935..1639)
                     /locus_tag="PLE93_04255"
     CDS             complement(935..1639)
                     /locus_tag="PLE93_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNH86293.1"
                     /translation="MADATVAEEEYLQTIFWLMEAGLPITGANIAKAMQLSPPTVHEM
                     IKRLERDGYVTRSADKILHLTEDGRAEAEQITRRHRMIERFLTDVLGIPWDEVHEEAE
                     RLEHAMSPVLEERMRAAIGEANTCPHGHPIDGVREEGAPLADVDLGAKVVVLRFENEA
                     ESLLHYLMDIGLYPGLSGTVTAKDDESVLIESHNGKHEVTRTVAETVTVRADPAPPPR
                     AALPDQLVLSDDRYGR"
     gene            complement(1664..2473)
                     /locus_tag="PLE93_04260"
     CDS             complement(1664..2473)
                     /locus_tag="PLE93_04260"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNH86294.1"
                     /translation="MSLRCVYTDLDGTLLGAGASLFTDEEGNFSMAQARALEAASRAG
                     VEVVIMSGRRRAQVHEDARIIGQNSFIFDAGAAFSIDQETTLLLGDFELEEGQTPVQR
                     MSERGVPDLLLENFAGRFEYHDPWHLDREMSHLFRGDIDTAEANALLEENGHGDLRLL
                     DNGAIHRRMEGIEQAHCYHLVPKAVSKVNAVAAHARARGYSPEETIAVGDSLEDLEVA
                     PAVGKFFIVANGPEKDPGVREAMSRYPNAVVTEASNGAGFYEAVVSSLMGA"
     gene            complement(2522..>2839)
                     /locus_tag="PLE93_04265"
     CDS             complement(2522..>2839)
                     /locus_tag="PLE93_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86295.1"
                     /translation="DQVPFACGYGVEIAMLIEVWGRVGIRGMAQVELGTKRNAHQSLA
                     ALSGMSSEVIAAMADTLDRLGRKDTGRMKMVEGSGPEVVTRPAFATVPQVSHRSGRKP
                     AAP"
BASE COUNT          529 a          933 c          876 g          501 t
ORIGIN      
        1 ttggacgcgc actacgccgt ccacccccgt ggccgagagc ccggatcctc tccagcgctt
       61 gtcgacatcg ccattcggct tgactctgaa gataactcca acggttctcg ccgatcccca
      121 gagagaatcc gcggcgatca atacacgtcc acgactatca atcgaaacat ctcgagtaac
      181 aattcctcct tgcccatgtc gaggcccata gcaatagagc cgatcctggg gcattcctcc
      241 agatccccag ccaggagcag agaaaccatc gaaacgaaac ctatccacca ttgaacaggg
      301 gaaggatgat cccccggaac cgccaagagt tatgtgtccg cgcctcccga tcgcgagaga
      361 agacctcgtg cggcacggct tcgttccctc gaccaagcca tttgtgccaa aagagtgctc
      421 aatcgcaccg ccgctcttga atgatgcgag gtaatagccc aggcaaccga attcctcccc
      481 gacccctcgg aggcttccca cgatcacaat gtcgccagaa cgagtgaagg caagagaagc
      541 tcccggattc agattctcgc cagctcgtac gcttagccca tggatcccga acgcgggatc
      601 aagacgcggc atggcaaccg cgctcaccgg gtgcacgcag aaggccaggg tcgagaaacc
      661 gaccaggaat gcgatgccga cccgaaaatg cttaagcaag cgagggtaca taatcaactc
      721 caggaagccg gggaccagaa tctggattca atgacatgaa gccattgctc cctcaacgca
      781 cagccgcggc aaaagtatcg ccatgtggga cccaaacaat gagcaagatc gggaagatga
      841 cccccgttcc aggcgatcct ggaccagcag gaacagccgc cacgcccggc tgcgcctttt
      901 ggctggccct ggtcgaccca actcgccgaa ccggctagcg gccgtagcgg tcgtctgaga
      961 ggacgagctg gtcggggagg gcggcccttg ggggcggggc ggggtcggcg cggacggtga
     1021 ccgtttcggc gacggttcgg gttacctcgt gcttgccgtt gtgcgattcg atcaggaccg
     1081 actcgtcgtc cttggcggta accgtgccac tgagccccgg gtagaggccg atgtccatca
     1141 ggtagtggag caggctctcg gcttcgtttt cgaaacggag cacgaccacc ttggcgccca
     1201 ggtcgacatc agccagcggt gctccttcct cgcggacccc gtcgatcggg tggccgtgcg
     1261 ggcaggtgtt ggcctcgccg atcgccgccc gcatccgctc ctccagcacc ggcgacatcg
     1321 cgtgctcgag ccgctcggcc tcctcgtgga cctcgtccca ggggatgccg agcacatcgg
     1381 tgagaaagcg ttcgatcatc cggtggcggc gggtgatctg ctccgcctcg gcccggccgt
     1441 cctcggtcag gtgaaggatc ttgtcggcgc tgcgggtcac gtagccgtcc cgctcaagcc
     1501 gcttgatcat ctcgtggacg gtgggcggtg agagctgcat cgccttggcg atgttggcgc
     1561 cggtgatcgg caatccggcc tccatcagcc agaagatcgt ctgcagatat tcctcttcgg
     1621 caaccgttgc gtcagccatg gcttgatcct agacctttgg ccttcacgca cccatgaggg
     1681 atgacacaac ggcctcgtag aagccggcgc cgttgctcgc ctcggtgacc accgcattcg
     1741 gatagcggct cattgcttcc cgcaccccgg ggtccttctc cgggccgttg gcgacgatga
     1801 agaacttccc gaccgccggg gcaacttcaa ggtcctcgag cgaatcgccg acggcgattg
     1861 tttcctccgg ggagtaaccc cgggcacggg catgggcggc gactgcgttc accttggaaa
     1921 ccgccttcgg aaccaggtga tagcagtggg cctgttcgat gccttccatc cggcggtgga
     1981 tcgcgccgtt gtcaagcagc ctgaggtcac cgtgaccgtt ctcctcgagc agtgcgttgg
     2041 cctcggccgt gtcgatatcc ccgcggaaga gatgtgacat ctcccggtcg aggtgccagg
     2101 gatcgtggta ctcgaagcgt ccggcaaagt tttcgagcag caaatcgggg accccgcgct
     2161 cgctcatccg ctggaccgga gtctggccct cctcgagttc gaagtcgccg agcagcaggg
     2221 tcgtctcctg gtcaatcgag aaggccgcgc ccgcatcgaa gatgaacgag ttctggccga
     2281 tgatccgggc gtcttcatgg acctgggccc ggcggcggcc ggacatgatc acgacctcca
     2341 ccccggccct cgaagcggcc tcgagcgccc gggcctgggc catcgagaag ttgccctcct
     2401 cgtcggtgaa gagcgaggca ccggccccga gcagggtgcc gtcgaggtct gtgtaaacgc
     2461 agcggagact catcgcgcct cacgctatcc gctgaggcac cgaagtggag ttcagacagg
     2521 actacggtgc cgcaggtttg cgaccgctgc ggtgcgaaac ctgcggcacc gtcgcgaagg
     2581 cgggtcgggt caccacttcc gggccgcttc cttcgaccat cttcatgcgc cccgtgtctt
     2641 tccggccgag ccggtccagg gtgtcggcca tcgcggcgat cacctcggag gacatgccgg
     2701 aaagggcggc cagcgactgg tgcgcgttcc gcttggtgcc gagctcgacc tgggccatgc
     2761 cacggatgcc gacccggccc cagacttcga tcagcatcgc gatctcgact ccgtagccgc
     2821 aggcgaacgg gacctgatc
//
LOCUS       DAOQFI010000188         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18222_length_2835_cov_3.109712, whole genome shotgun sequence.
ACCESSION   DAOQFI010000188 DAOQFI010000000
VERSION     DAOQFI010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18222_length_2835_cov_3.109712"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLE93_04270"
     CDS             complement(<1..489)
                     /locus_tag="PLE93_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86296.1"
                     /translation="MKSSVPFRAAVAAVVLLAAVALAIDGCGKDEETETTRTLRIYVS
                     QPLKGDRGGEDMLRAVEIANEDEGGTIGPARIEIVGMNDSDESGNWVASLVRRNARRA
                     AADDSAVAYIGDFDSGATEVAMPILNRAGMLQVSSSATAVKLTKPLPELGEHIRPTGI
                     RTF"
     gene            complement(554..1123)
                     /locus_tag="PLE93_04275"
     CDS             complement(554..1123)
                     /locus_tag="PLE93_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNH86297.1"
                     /translation="MTDWVFGYGSLVTDLVTSSDQNGFEWFWANLGNHRRDWGAGMEN
                     AAADADWKYYVDSRGTRPDIVVAFLDAYPHSGSSINGVVFAVDESRLREIDRREVRYS
                     RVDVTDLVTTAGWGDGRVWTYRGSEKSREVALRGRREDRAFVSRDYAAHCRHGFSLGG
                     EQALHQFQLSTDPLDLPLRELTMVLSDPG"
     gene            complement(1120..2037)
                     /locus_tag="PLE93_04280"
     CDS             complement(1120..2037)
                     /locus_tag="PLE93_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86298.1"
                     /translation="MVGEPPRPKQPPGRIGTLARLFHPKALNRKHPHVSDRLRTFMAT
                     LTFLELVLIFVLLIVASTVLLGLTFWWLAPSQLYNSYWDALGSAFFTLIGMPPGYLDP
                     AELSGRFKVAVGAAGLFSLLLPALFLGAIVFRLFVRLRIFVLKERPTLTRKKVRGTEG
                     WYLAIRLYSSTAILIVDIDFKVYLRMKSSNGLLLSNEEIRVTNATWPIADTHVPYTLY
                     LPVDDGDLDANEHLISLQGHDLETCESAIIHIRGASPQLASEVNETHTLEVPLDLIPA
                     DFGGVQVLYDGRGPDWVGWDEFEADRPLQ"
     gene            2261..2593
                     /locus_tag="PLE93_04285"
     CDS             2261..2593
                     /locus_tag="PLE93_04285"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNH86299.1"
                     /translation="MNQNSLHPGFTPNVVAIVGNQRPDSRTLRLASGIADGLAAQLGD
                     TPAVAIDLVNLPLENGGFSPGLNDLIRDSRVVIAASPTVMGSYSGLLKIFLDGLPEAA
                     LLDKTGIP"
     gene            2593..2832
                     /locus_tag="PLE93_04290"
     CDS             2593..2832
                     /locus_tag="PLE93_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86300.1"
                     /translation="MIARQARHALAADVHLRPALLELGATCPTPSLVALESELASLGA
                     DGLVTAWLERSGNWLQLSDGSAAGCSPRRHRHLTT"
BASE COUNT          555 a          890 c          869 g          521 t
ORIGIN      
        1 gaaggttctg atcccggtcg gccgaatatg ttcaccgagt tcgggcaggg gcttggtcag
       61 cttgacggcg gtggcgctgg agctgacctg gagcatcccg gcgcggttga ggatcggcat
      121 cgcgacctcg gttgcgccgg aatcgaaatc accgatgtag gcgacggcgg agtcgtcggc
      181 ggcagcgcgc cgcgcgttgc ggcggaccag cgaagcgacc cagttgccgc tctcgtcgga
      241 atcgttcatg ccgacgatct cgatccgggc cgggccgatc gtcccgccct cgtcctcatt
      301 cgcaatctcg accgcccgca gcatgtcctc gccgccgcgg tcacccttca gcggctggct
      361 tacatagatc cgcagggtgc gggtcgtttc ggtctcttcg tccttgccgc agccgtcgat
      421 ggccagcgca accgcggcga gcagaactac ggccgcgacc gcggcccgga acgggacact
      481 tgacttcaag atgccggact attccagttc gtcggccctc acgttgaaac cgaaccgggc
      541 tgacgcgcaa gagtcaacca ggatccgaaa gaaccatcgt gagttcgcgg agtggcagat
      601 cgaggggatc cgtgctcaat tgaaactggt gaagtgcctg ctcacctccc agggagaatc
      661 cgtgccggca atgcgctgca taatccctgc ttacgaaggc ccggtcttct cgccttcctc
      721 ggagtgcgac ttcgcgagat ttctcgctcc cccggtaggt ccagacccga ccgtctcccc
      781 aaccggccgt cgtgaccaga tcagtcacgt cgacgcggga gtacctcact tcgcgccgat
      841 caatttcccg gagacgactc tcgtctacgg caaatacgac tccgttgatg ctggaaccgg
      901 agtgggggta agcgtcgaga aaagcgacca cgatgtcagg gcgtgtgcct cgagagtcga
      961 cgtagtactt ccaatcggca tcggcggcag cattctccat gccggcaccc caatcccgcc
     1021 ggtggttgcc aagatttgcc cagaaccact caaagccatt ctggtccgaa cttgtgacca
     1081 ggtcggtgac gagcgatccg tagccgaaga cccagtcggt cattgcaacg gtcggtctgc
     1141 ttcgaattcg tcccagccca cccaatccgg gccacggccg tcgtagagaa cctggacccc
     1201 gccaaaatcc gccggaatga gatccaacgg aacctccagc gtgtgcgttt cattcacttc
     1261 cgacgcaagc tgtggggacg cgcctcggat gtggatgatc gcgctttcgc aggtctctag
     1321 atcgtgaccc tgcagtgaaa tgaggtgctc gttggcatca agatcgccgt cgtccacggg
     1381 aaggtaaagg gtgtagggga catgagtgtc ggcgatgggc caggtggcgt tggtcacccg
     1441 gatctcttcg ttcgagagca gtaatccgtt cgagctcttc atccgcaagt agaccttgaa
     1501 atcaatatcg acgatcagga tcgccgtcga cgagtacaga cggatcgcca gataccagcc
     1561 ctcggtgcct ctcactttct ttcgggtgag tgttggtctt tccttgagga cgaagatgcg
     1621 cagccggacg aaaaggcgga agacaatggc tccgaggaaa agggccggca aaagcaacga
     1681 gaagagtcca gccgccccta cggcgacctt gaatcttcca ctcaattctg cagggtccag
     1741 atagccgggt ggcatgccga tcagggtgaa aaaggctgat ccaagcgcgt cccaataact
     1801 gttgtagagc tggcttggag caagccacca gaaggtgagt cccagaagga ccgtcgatgc
     1861 gacgatcaac agaacgaaga tcagaacgag ctccagaaaa gtcagagtcg ccatgaatgt
     1921 cctcaagcgg tcggaaacat gaggatgctt gcgattcagg gccttcggat ggaaaaggcg
     1981 ggccagggtc ccaattcgtc ctggcggctg cttgggcctt ggcggttcgc caaccattgt
     2041 ccgatcctat tcgggaattg agcgggcaga ctcctgccca ttgctggtct tctggtcgaa
     2101 catgtagtat cttgatttat tgactagaca aactcatgaa tgtctatgtc gactccgctg
     2161 ggcggtgggc tagacggccc ggctggacgc aaggcaaggg ggccgggccg aaaggtccgg
     2221 cccccgaccc tccttccccc ggtcaagaca ggacgatccg atgaaccaga actccctcca
     2281 tcccggattc acccccaacg tcgtcgccat cgttggcaac cagcgcccgg acagccgcac
     2341 cctcaggctg gcctccggca ttgccgacgg gctggcggct cagctcggcg acaccccggc
     2401 ggtcgcgatc gacctggtca acctgccgct ggaaaacggc ggcttcagcc cgggactgaa
     2461 cgacctgatc cgggactccc gggtcgtgat cgcggccagc ccgaccgtga tgggcagcta
     2521 ctcgggcctg ctcaagatct ttctcgacgg cctgcccgag gcggcgctgc tcgacaagac
     2581 cgggatcccc tgatgatcgc gcgccaggcc cgtcacgccc tggcggccga cgtccacctg
     2641 cgaccggccc tgctggaact tggcgcgacc tgcccgaccc cgtcgctggt cgcgctcgag
     2701 tcggagctcg cctcactggg agccgacggt ctggtcaccg cctggctgga acggagcgga
     2761 aactggctgc agctcagtga cggatcagca gccgggtgct cgccgaggcg tcatcgccat
     2821 ttgacgactt gagcg
//
LOCUS       DAOQFI010000189         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18358_length_2822_cov_2.990604, whole genome shotgun sequence.
ACCESSION   DAOQFI010000189 DAOQFI010000000
VERSION     DAOQFI010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18358_length_2822_cov_2.990604"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLE93_04295"
     CDS             <1..436
                     /locus_tag="PLE93_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86301.1"
                     /translation="SRSGGMTTEISSSLTAAGLGVSTAVSIGGDAIIGSSYAELMPYF
                     EADEETRAIVIYTEPGGRMEAQLADWQAENNSRLPIVAFMAGKFMDDESMKGMTFGHA
                     GTIVEGKEDSATEKIKRLESAGITVVERIDEIPDAVKERIGA"
     gene            433..669
                     /locus_tag="PLE93_04300"
     CDS             433..669
                     /locus_tag="PLE93_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86302.1"
                     /translation="MSNETMDSVFIDIELDEPAASDPELAKKLEEVCTVGIFKATENG
                     TEIVRGQLDECVLCYLCEEAAPEGSLRIIKKYEN"
     gene            905..2356
                     /locus_tag="PLE93_04305"
     CDS             905..2356
                     /locus_tag="PLE93_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH86303.1"
                     /translation="MNFIDDVIQKFPFSQEAVIAIDGEGNRTVFCFSHLFARSLGLSG
                     AMLDRGVRRGDVVMILVGSRIEWVVSMLACFRMGAIALPLNPQLSEHDLAYRVAATNP
                     ALAIGEADYLAGLPDGIPAFDLEDLDRIFDEDLAQATPAKVASLALDDPALIVFTSGT
                     TAEPKGVVHSQRYLMGQAVQAQHWVGAEPDDLCWCTAAPGWSKSSRNSFIAPWLRGAK
                     SLLVEGRFDPATRMDIVRQQEVNVLCQAPTEYRILAKRTTLSPAPSLRRMVSAGEALN
                     PEVIGAFREATGLEIADGYGQTETGAVTGMRPGEAVAGREGSMGRPLPGIETRIVEGE
                     LQVMAATSPTFFHSYIDGSRFEGEWWATGDLVREDDEGYLFHEGRNDDVISSAGYRIG
                     PVEVESALLSHPAVAEAAAIASPDPERGSVVKAVVVLRDRHGDEALAAELQEHCRRET
                     APYKYPRLIEFTDELPKTASGKIRRAELRDRET"
     gene            2494..>2822
                     /locus_tag="PLE93_04310"
     CDS             2494..>2822
                     /locus_tag="PLE93_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH86304.1"
                     /translation="MSDSGLDPQRIASLTEAQQEIYRNRTGKSAAEYQRAVKVMPGGV
                     PSSFQMMDPWPVYLTEGKGSQVWDVDGNQYTDFHAGFGVMVIGHANPTIGEAVKARID
                     QSTHFAAP"
BASE COUNT          523 a          929 c          912 g          458 t
ORIGIN      
        1 gtcccgctcc ggcggcatga ccaccgagat ctcctcctcg ctcacggccg ccggcctggg
       61 cgtctcgacc gccgtctcga ttggcggcga cgcgatcatc ggctcgagct acgccgagct
      121 tatgccgtac ttcgaggctg atgaggaaac cagggcgatc gtcatctaca ccgagccggg
      181 tggccgcatg gaagcccagc tcgccgactg gcaggccgag aacaattccc gcctgccgat
      241 cgtcgctttc atggccggca agttcatgga cgatgagtcg atgaagggca tgaccttcgg
      301 ccatgccggc accatcgtcg agggcaagga agactcggcg accgagaaga tcaagcgtct
      361 ggaatcggcc ggcatcaccg tggtcgagcg gatcgacgag atccccgacg cagtcaagga
      421 aaggatcgga gcatgagcaa cgaaacgatg gacagcgtct tcatcgacat cgagctcgac
      481 gagcccgccg cctcggatcc cgagctggcc aagaagctgg aagaggtctg caccgtcggc
      541 atcttcaagg cgaccgagaa cggcaccgag attgttcgcg gccagctcga cgagtgcgtc
      601 ctctgctacc tctgtgaaga agccgctccc gagggatccc tgcggatcat caagaaatac
      661 gagaactagc ttcggttaga gcgggtccgg gcagtacacc cctcctcggc ccgaaacgtc
      721 gtcggggcgc actgcccgga cccgctcaac caaactcgtt cgaaagggtg gcccgaaacg
      781 tcgtcggcgg gcactcccgc aaccaggtcg cccgggcatc tcgattgtcc gacccgaaac
      841 tcgtcggggc gcactgaccg gacccggtca cgcaagcgcc aaggctggct agactcggcg
      901 gaccttgaac ttcattgatg acgtaatcca gaagttccct ttttcgcagg aggccgttat
      961 tgcgattgac ggtgagggga accggacggt cttctgtttt tctcaccttt ttgcgcggag
     1021 tctgggcctt tcgggggcga tgctggaccg gggagtgcgg cgcggggatg tcgtgatgat
     1081 cctggtcgga agcaggatcg agtgggtcgt ttcgatgctg gcctgcttcc ggatgggggc
     1141 gattgcgctg ccgctcaacc cccagctcag cgagcacgac ctcgcctacc gggtcgccgc
     1201 caccaatcct gctctggcga tcggcgaggc cgactacctg gccggcctgc cggacgggat
     1261 acccgccttc gatctggaag acctcgaccg gatctttgac gaggacctcg cccaggcgac
     1321 gccggccaag gttgccagcc tggctctgga tgacccggcc ctgatcgtct tcacctccgg
     1381 caccaccgcc gaaccgaagg gcgtagtcca ctcgcagcgc tatctgatgg gccaggccgt
     1441 tcaggcccag cactgggtcg gggccgagcc ggacgacctt tgctggtgca ccgccgcccc
     1501 gggctggtcg aagtcttcgc gaaactcctt catcgccccc tggctgaggg gagcgaagtc
     1561 gctgctggtc gagggtcgtt tcgaccccgc cacaaggatg gacatcgtcc gccagcagga
     1621 ggtcaacgtg ctctgccagg ccccgaccga gtaccggatc ctggccaagc gaaccactct
     1681 ctcccccgcc ccctccctac ggaggatggt ctcggccggc gaggcgctga accccgaagt
     1741 gatcggtgcc tttcgcgagg cgaccggact cgagatcgcc gacggttacg gccagaccga
     1801 gaccggcgcc gtgaccggca tgcggcccgg cgaggccgtg gccggtcgcg aagggtcgat
     1861 gggccggcct ctgcccggta tcgagacccg gatcgtcgag ggcgaactcc aggtgatggc
     1921 agccacctcc cccaccttct tccacagcta catcgacgga agccgcttcg aaggcgagtg
     1981 gtgggcgacc ggcgacctgg tccgcgagga cgatgagggt tatctctttc acgaaggccg
     2041 gaacgatgac gtgatctcct cggccggcta ccggatcggt ccggtcgagg tggaatcggc
     2101 gctgctctcg catcccgcgg tggccgaggc ggcggcaatc gcctcgcccg acccggaacg
     2161 cggttcggtc gtgaaagccg tggtggtgct gcgcgaccgc cacggtgacg aggccctggc
     2221 cgccgaactg caggaacact gccgccggga aaccgcgccc tacaagtacc cccggctgat
     2281 cgagttcacc gacgagctcc cgaaaacggc ctccggcaag atccgaaggg cagaactcag
     2341 ggacagggaa acctgaccgc aagacctgaa gatggccggg tcggctctgc ttgtcaaaat
     2401 gtccagcccg gtccggaacc tctgacgttt gagtctggat cggcggcggg cagatatata
     2461 ggctgatccc taccccacga ccgaggagag cagatgtctg acagcggcct tgacccccag
     2521 cgaatagcct cactgaccga ggcgcagcag gagatctatc gcaatcgcac cggcaagtcc
     2581 gccgcggaat accagcgcgc ggtgaaagtg atgccggggg gagtgccttc ctccttccag
     2641 atgatggacc cctggccggt ctacctgacc gagggcaagg gatcccaggt ctgggacgtc
     2701 gacggcaacc agtacacgga cttccacgcc ggtttcggcg tgatggtgat cggccacgcg
     2761 aatccgacga tcggcgaggc ggtcaaggcc cggatcgacc agagcaccca ctttgccgcc
     2821 cc
//
LOCUS       DAOQFI010000190         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18362_length_2822_cov_2.793639, whole genome shotgun sequence.
ACCESSION   DAOQFI010000190 DAOQFI010000000
VERSION     DAOQFI010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18362_length_2822_cov_2.793639"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="PLE93_04315"
     CDS             <1..1258
                     /locus_tag="PLE93_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH86305.1"
                     /translation="GCGFGLGLEILRQNYAHLELTGLEWDYDCAREAERVTRAVVVVG
                     LPEETSYRNRFDLAVFHRALALCQDPELALRKAVESLRKGGMLVIASESESADSVVPF
                     QPALDAAFALGRGEFRSGRTTDVLLSAYRIGARAAWREWSKVAIGAEIPIEFFARFAE
                     MIFAYIDQLSASSVAGYSHELEASQRSRSRRFEELARRILAGGPAETLQAEAERVGWD
                     APEAVSAVILANARAQGIRNLVDSRSLIVPGNLPGLEDEDLMLLIVPTVRTSERERIK
                     DQLAGRGAVIGPSREWRRAGQSYARAVKVLNLNRGGHRDDPADTDDFLVELVVGADPE
                     ALEDLRREVLSPLEELRPSTAEKLRTTLEAWLLHQGRRDGIAAALHVHPQTVRYRMGQ
                     LREIYGDRLDDPAMVLAATVALAAKN"
     gene            complement(1299..>2822)
                     /locus_tag="PLE93_04320"
     CDS             complement(1299..>2822)
                     /locus_tag="PLE93_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH86306.1"
                     /translation="GAIVTAAALGLLAPAASAGTVRKPAGTPRELRANAGGGVAANVN
                     MTNSRKVISTGIDSGNVADLAPAWTFPLTSGGIFGAMSSNPIVSDGVLYFSDAASNVY
                     ALDAFTGIQKWKRTFNVAGLGQNGVTLANGRLLAGVDSDVLSLDATTGKTIWRTTLNA
                     SDSAAVDMAPLAWKDLIIVSTIPFKIGTGIYKGGQKGVVFGLNAKTGRKVWSFDTTTD
                     NLWGNPAVNSGGGLWSTPSIDDHGTMYMSVANPGPLPGTVDFPNGSSRPGRNLYTNSL
                     VALNAATGKLKWFFQAVPHDFRDYDLQLPPILATFRVNGRKRDVVITGGKMGTVYVVD
                     RRNGKLIWKRNVGRHNQWGDPARDLPTAAEDLPVTMYPGVAGGVETPMSVSGNSIFVP
                     VSDLCATVVSVTTIVSCPYQTGTGAILALGGGTGRTLWKTPIPTLPDGATTVVGDVVM
                     APGVDGTLYSLRASDGEILASPKAITGVNAPPAVSGRTVYIGAGAGPGPAVQAWRLP"
BASE COUNT          448 a          933 c          953 g          488 t
ORIGIN      
        1 tggctgcggc ttcggtctgg ggctggaaat tttgcgccag aactacgccc acctggaact
       61 gaccggtctg gagtgggact atgactgtgc tcgcgaggcc gagcgagtca ctcgggccgt
      121 ggtggtggta ggtctgcccg aggaaacgag ctatcgcaac cggttcgatc tggccgtctt
      181 ccaccgagcc ctggccctct gccaggatcc tgaactggcc ctgcgcaagg cggtcgagag
      241 cctgcgcaag ggtgggatgc tggtcatcgc ctcggaatcc gaatcagccg attccgtggt
      301 tccgttccag cctgcccttg acgccgcctt tgcccttggt cgaggcgagt tccggagcgg
      361 ccgaactacc gacgtgctcc tctccgcgta ccggatcggg gccagggcgg cctggcggga
      421 atggtcaaag gtggcgatcg gggctgaaat cccgatcgag ttcttcgccc gattcgccga
      481 gatgatcttc gcctacatcg accagctttc tgcttccagc gtggccggct actcccacga
      541 gctggaagcc agccagcggt cccggagccg gcgcttcgag gagcttgccc gcaggatcct
      601 ggctggcgga ccggccgaga cccttcaggc cgaggcggaa agggtcggct gggatgcccc
      661 ggaagcggtg tccgccgtga tcctcgccaa cgccagggcc caggggatcc ggaacctggt
      721 cgattcgcgc agcctgatcg ttcccggcaa cctgcccggc ctcgaggacg aggacctcat
      781 gctgctgatc gttccgacgg tccggaccag cgagcgcgag cggatcaagg atcagctggc
      841 cggccggggt gcggtgatcg gaccgtcccg tgagtggcgg cgggccggtc agtcctacgc
      901 ccgggcggtc aaggtgctca acctgaaccg cggcgggcac cgggatgacc cggccgacac
      961 cgacgacttc ctggtcgaac tggtggtcgg ggccgacccc gaagcccttg aggacctcag
     1021 aagggaagtc ctctcccccc ttgaggaact tcgtccctcg accgcagaga agctccggac
     1081 cacgcttgaa gcctggctgc tccaccaggg tcgccgcgac ggtatcgccg ccgcccttca
     1141 cgttcatccg cagacggttc gctaccggat ggggcagctg agggaaatct acggcgaccg
     1201 gctggacgat cccgccatgg tcctcgccgc cacggttgcc ctggccgcca aaaattgaag
     1261 tcccggggtc gaagtgcccg gccggccgat cgccccgctc agggcagtcg ccaggcctgg
     1321 accgccgggc cggggcccgc tccggcaccg atgtagacgg ttcggccgga aactgccggc
     1381 ggtgcgttga cgccggtgat cgccttcgga gaggcgagga tttcaccgtc actggcccgc
     1441 agcgagtaaa gggtcccgtc cactcccggg gccatgacga catcacccac caccgtcgtc
     1501 gctccgtccg gcagggtcgg gatcggggtc ttccagagcg tccttccggt gccgccaccg
     1561 agggccagga tcgctccggt cccggtctgg tagggacagg agacgatggt cgtcaccgag
     1621 acgaccgtcg cgcagaggtc ggacaccggc acgaagatgg aattgccgga aaccgacatc
     1681 ggggtttcga cgccgccggc gacccccgga tacatggtga ccggcagatc ctccgccgcg
     1741 gtcggaaggt ccctggccgg gtcgccccac tggttgtgcc tgccgacgtt tcgcttccag
     1801 atcagctttc cgttccggcg gtcaaccacg tagacggttc ccatcttgcc gccggtgatg
     1861 accacatccc gtttccggcc gttcaccctg aaggtggcga gaatcggagg cagttgcagg
     1921 tcgtagtccc ggaagtcatg cggaacggcc tggaagaacc atttgagttt gccggtcgcc
     1981 gcgttcagcg cgaccaggct gttcgtgtag agattcctgc cgggccggct cgaaccgttg
     2041 gggaaatcga ccgtccccgg cagcggcccc ggattggcga cgctcatgta catcgttccg
     2101 tggtcatcga tgctgggggt gctccagagc cctcccccgc tgttgaccgc ggggttgccc
     2161 cagagattgt cggtcgtcgt gtcgaaagac cagaccttgc gaccagtttt cgcgttgagc
     2221 ccgaagacga cgcccttctg cccgcctttg tagatcccgg tcccgatctt gaacgggatc
     2281 gtcgagacga tgatcaggtc cttccaggcg agcggggcca tgtccacggc ggcgctgtca
     2341 ctcgcgttca gggtggtgcg ccaaatggtt ttgccggtcg tcgcgtcaag gctcagcaca
     2401 tcggaatcga caccggccag caggcgtccg ttcgcgaggg tcacaccgtt ctggcccagt
     2461 ccggccacgt tgaaggtgcg tttccatttc tggatcccgg tgaaggcgtc cagcgcatag
     2521 acgttcgatg ccgcgtccga gaagtagagg accccgtcgc tgacgatcgg gttcgaagac
     2581 atcgcgccga agatgccgcc ggaagtgagc gggaaggtcc aggccggggc aaggtccgcc
     2641 acgttgcccg agtcgatccc ggtcgagatc accttccggg agttggtcat gttgacgttc
     2701 gcggcgaccc cgccaccggc attggcccgc aactcgcgcg gcgtcccggc cggtttccgg
     2761 accgtgccgg cggacgccgc cggggcgagc aggcccaggg cggcggcagt gacgatcgcg
     2821 cc
//
LOCUS       DAOQFI010000191         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18392_length_2819_cov_3.219609, whole genome shotgun sequence.
ACCESSION   DAOQFI010000191 DAOQFI010000000
VERSION     DAOQFI010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18392_length_2819_cov_3.219609"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2819)
                     /locus_tag="PLE93_04325"
     CDS             complement(<1..>2819)
                     /locus_tag="PLE93_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNH86307.1"
                     /translation="RLVYVSPLKALSYDIERNLRAPLKGIGVDLSVGIRTGDTSQTER
                     NRMRREPPEILITTPESLYLMLSSGARDILTSVETVIVDEIHAVAATKRGAHLALTLE
                     RLEHLVQENAGHGVQRIGLSATQRPLERIAGFLSGPGRECEIVDPGQRKELDLEIVVP
                     VEDMTAPEADQHDDGSADDAPIRSIWPAIYPELLEQVRAHTSTIVFVNNRRAAERLAQ
                     RLNDLDQETGEIESPNRTATEGPRPDQAGQPASGPEDGPKRPHEVEGLPLARAHHGSL
                     SHEERTEVEEQLKSGLIPCLVATSSLELGIDMGAVDLVVQVESPKSVARGLQRIGRAG
                     HGLGETSKGRIFPKFRGDLLESAVVTRRMREGLIEETVIPSNPLDVLAQHLVSMAALD
                     KWDTTDAYELVRRTTPFAELSIEQFDNVLDMLDGRYPSDRFAELRPRLVWDRTGKTIE
                     GRKGARSLVVTNAGTIPDRGLYGVHLPDGRRVGELDEEMVYEARAGQVFLLGASSWRI
                     QEITRDRVIVIPAPGVPGAIPFWRGDGIGRPAELGEAIGAFAREAVNAEPAELEKSHD
                     LDPRAARNLIAYLKDQQDATRVVPSDQTVVIERFRDEIGDWRLVVLTPWGGRVHAAWG
                     LAIARKLRDEHGLEADAIHSDDGIAVHLPDADEPPSADLVLIEPDELEDLVVRELGSS
                     ALFGARFRENAARSLLIPRAYPGKRTPLWQQRLKSQSLLEVAQDFPRFPVILETYREC
                     LQDVFDLPALDRLLRGIHSREISLVEVETQTASPFASSLLFDYVATYMYEGDTPNAER
                     RATALSLDRELLRELLGQDELRELIDEGALAEVEASLQHLTEHGRAADRDGLQQILRR
                     LGDLTAEEAEARVAEGYSAGSMLENLISERRAVKVRINGEERFIAAEDAGLYRDALGV
                     APPGGLPADFLEERPDAMETLLSRY"
BASE COUNT          413 a          932 c         1019 g          455 t
ORIGIN      
        1 cgtagcggct cagcagggtt tccatcgcgt ccggtcgttc ctcgaggaag tcggccggca
       61 gccccccggg cggagccacg ccgagagcgt cccggtaaag accggcatcc tcggcggcga
      121 tgaaccgctc ttcaccgttg atccggacct tgaccgcgcg gcgctcggag atcaggtttt
      181 ccagcatcga accggccgaa tacccttccg cgacgcgggc ctcggcctct tcggcagtca
      241 ggtcgccgag ccggcggagg atctgctgga ggccgtcgcg gtcggcggcc cggccgtgct
      301 cggtcaggtg ctggagcgat gcctcgacct cggccagggc tccctcgtcg atcagctcgc
      361 gcagctcgtc ctggccgagc agctcgcgca ggagttcgcg gtccagcgaa agagcggtgg
      421 cccggcgttc cgcgttcggg gtgtccccct cgtacatgta ggtagcgacg tagtcgaaga
      481 gcagcgagga ggcgaagggc gaggcggtct gggtctcgac ctcgaccagc gagatctccc
      541 gcgagtgaat gccgcgcagc agccggtcca gcgcgggcag gtcgaagacg tcctgcaggc
      601 actcgcggta ggtctcgagg attaccggga agcgggggaa gtcctgggcg acctcgagca
      661 ggctctggga cttgagccgc tgctgccaga gcggtgtccg cttgccgggg taggcgcggg
      721 ggatcagcag cgaacgggcc gcgttctcgc ggaagcgggc cccgaagaga gcggaggaac
      781 cgagttcgcg gaccacgagg tcctcgagtt cgtccggttc gatcaggacc aggtcagccg
      841 agggcggctc gtcggcatcg ggcaggtgga cggcgatgcc gtcatcggaa tggatcgcgt
      901 cggcttcgag cccgtgctcg tcgcggagct tgcgggcgat cgcgaggccc caggccgcgt
      961 gcacccgtcc gccccagggg gtgagtacga ccagccgcca gtccccgatc tcgtcgcgga
     1021 agcgctcgat cacgaccgtc tggtccgagg gcaccacccg ggtcgcgtcc tgctggtcct
     1081 tcaggtaggc gatcaggttg cgggctgccc gcggatcgag atcatgggac ttctccagct
     1141 cggccggttc ggcgttgact gcctcgcggg cgaaggcgcc gatcgcttcg cccagttcgg
     1201 ccggccggcc gatcccgtca ccgcgccaga acgggatcgc ccccggcaca cccggggccg
     1261 ggatcacgat cacccggtca cgggtgatct cctgaatccg ccagctcgag gcgccgagca
     1321 ggaagacctg gccggcccgt gcctcgtaga ccatctcctc gtcgagctcg ccaacccggc
     1381 ggccgtccgg caggtgaacc ccgtagaggc cgcggtcggg gatcgtgccg gcgttggtca
     1441 cgaccagcga gcgggcgccc ttgcgtcctt cgatcgtctt gccggtccgg tcccagacca
     1501 ggcgcggccg gagctcggcg aaacggtccg acgggtagcg gccgtcgagc atgtcgagca
     1561 cgttgtcgaa ctgctcgatc gagagctcgg cgaagggcgt ggtgcggcga accagctcgt
     1621 aggcatcggt cgtgtcccac ttgtcgagcg ccgccatcga aaccaggtgc tgggcgagca
     1681 cgtcgagcgg attcgacggg atcaccgtct cctcgatcag gccttcccgc atgcggcggg
     1741 taaccaccgc gctttcgagc aggtcgcccc ggaacttggg gaagatccgc cctttcgagg
     1801 tttcccccag gccgtggccg gcccggccga tccgctgcag gccgcgcgcg accgacttcg
     1861 gcgattcgac ctggaccacg agatcgaccg cgcccatgtc gatcccgagc tcgagcgagg
     1921 aggtggcgac caggcagggg atcagcccgg acttgagttg ctcctcgacc tcggtccgct
     1981 cctcgtggga gagcgaaccg tgatgggccc gggccagggg caggccctcc acctcgtggg
     2041 gccgcttcgg tccgtcctcc ggcccggacg ccggttgacc ggcttgatcc gggcggggcc
     2101 cttcggtcgc cgtgcggttc ggcgattcga tctcgccggt ttcctggtcg aggtcgttca
     2161 gccgctgggc gaggcgctcg gcggcacggc ggttgttgac gaagacgatg gtcgaggtgt
     2221 gagcgcggac ctgctcgagc agctccggat agatcgccgg ccagatcgac cggatcgggg
     2281 catcgtccgc ggagccatcg tcgtgctggt cggcttcggg tgcggtcatg tcctcgaccg
     2341 gcaccacgat ttccaggtcg agttccttgc gctggcccgg gtcaacgatt tcgcattcgc
     2401 gaccggggcc ggaaaggaag ccggcgatgc gctcgagggg ccgctgggtg gccgagaggc
     2461 cgatcctctg caccccgtgg ccggcgtttt cctgaacgag gtgttccagc cgctcaaggg
     2521 tcagggcgag gtgggcgccg cgcttggtcg cggcaacagc gtgaatctca tcgacgatca
     2581 cggtctcgac cgaggtgagg atgtcccggg cgcccgagga aagcatcagg tagagagact
     2641 ccggggtggt gatcaggatc tccggcggtt cgcgccgcat ccggttgcgt tcggtctggg
     2701 aagtgtcccc ggtccggatc ccgaccgaaa ggtccacccc gatccccttc agcggggcgc
     2761 gcaggttgcg ctcgatgtcg taggaaagcg ccttgagcgg cgatacataa accagcctc
//
LOCUS       DAOQFI010000192         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18456_length_2814_cov_3.053280, whole genome shotgun sequence.
ACCESSION   DAOQFI010000192 DAOQFI010000000
VERSION     DAOQFI010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18456_length_2814_cov_3.053280"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLE93_04330"
     CDS             complement(<1..711)
                     /locus_tag="PLE93_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNH86308.1"
                     /translation="MNSDGNRKATGHRIRAIGALLLALFAIGHFTSEASAGTRPLKTG
                     VTVIDFTNTSEAGYKRIRSTGATLTRVIITWPLVVGSKAPANPSDPADPAYSWGHYDT
                     QIQNAVNAGLTVLVQINGAPKWAERCKTQSTTATCNPNPVAFARFARAAATRYSGTYE
                     GLPRIRYWEPLNEPNLESNFLPQRRSSGKQSPVLYRSVLNRFAAAVKGVDPTNLVVGG
                     GLSPLGGGYFADAIGIQEK"
     gene            796..2088
                     /locus_tag="PLE93_04335"
     CDS             796..2088
                     /locus_tag="PLE93_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH86309.1"
                     /translation="MRSTDLLQELIRFNTVNPPGNEGPALEHIMSFLDPAGWDCKFLA
                     KVPERPNLVARLKGVEPGPNLALISHVDTVPADPEEWSHDPWSGNLADGYIWGRGALD
                     MKDQVAAEVAACVNLARSGWRPARGDLILVVAADEETGAHFGAKWLCEEHPDEVRSDW
                     VLNEGAGELLEFDGRRLFTLSIGEKGTFRFTVITEGEAGHGSIPRVGDNALLKLAPAL
                     SNAHRQPPHESTPDTDLFLERLLGHPVEDTDAALREIEAIDPLVAKLLADPMLGVTIA
                     PTMASASSRENVIPSRAELVIDCRTPPGMTEEQVRARIDLVLGDGDWRIEFNDPVVGN
                     RSDYDGPLAEAIEEWVGLTEPGAEVLPQVMPGFSDSHWFRKAFGATVFGFAPQRMSIA
                     ETKPLVHGKDERITVEDVELMENLYTWIPPRLLGTAES"
     gene            complement(2285..2674)
                     /locus_tag="PLE93_04340"
     CDS             complement(2285..2674)
                     /locus_tag="PLE93_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86310.1"
                     /translation="MEQKVEGSTETRGSERLGWKGLFTNHGPKFEVSRLVHQIVFGLF
                     LILGFVPVFLMSSHIHYPWNASFLEELAPMIVFMVYGAGLFSGAGRFTRWLFGPMPKR
                     EPYPTSPLPPQYSGQDQVSKETKPKDE"
BASE COUNT          511 a          899 c          900 g          504 t
ORIGIN      
        1 cttttcctgg atgccgatgg cgtcggcgaa gtagccgccg ccgagcgggg aaagaccgcc
       61 gccgaccacc aggttggtgg ggtccacgcc cttgaccgcg gctgcgaacc ggttcagcac
      121 cgaccggtaa aggaccgggg actgcttgcc cgagctgcgc ctctgcggca gaaagttcga
      181 ttcgaggttg ggctcgttaa gtggctccca gtagcggatc cggggcagcc cttcataggt
      241 gccgctgtat cgggtcgctg ccgccctggc aaaacgagcg aaggcgaccg gattcggatt
      301 gcaggtagcc gtggtgctct gggtcttgca gcgttcggcc catttcggcg ccccattgat
      361 ctggaccagc accgtcagcc cggcgttcac cgcgttctgg atctgggtgt cgtagtgccc
      421 ccagctgtag gccgggtcgg ccgggtcaga cggatttgcc ggagctttcg agccgaccac
      481 gagcggccag gtgatgatca cccgggtcag ggtggcaccg gtgctgcgaa tccgcttgta
      541 gccggcttcg ctcgtattgg tgaagtcgat caccgtgact ccggtcttca gcggacgggt
      601 tccggccgag gcctcgctcg tgaaatgacc gatcgcgaaa agggcgagca ggagcgcacc
      661 gatcgcgcgg atccggtgtc cggtggcctt cctgtttcca tcggagttca tgggactggg
      721 gattatttca acaggcatcg ggatctataa accacgtcga ccggataagt accgccgctg
      781 tgagatcctg cgcctatgcg ctcgaccgac ctgcttcagg agctgatccg cttcaacacg
      841 gtgaatccgc ccgggaacga gggcccggcg ctggaacaca tcatgagctt tctcgacccg
      901 gccggctggg actgcaagtt cctggcgaag gtgccggagc ggcccaacct ggtggcgcgg
      961 ctcaagggcg tcgaaccggg acccaacctc gccctgatca gtcatgtgga caccgtcccg
     1021 gccgatccgg aggagtggtc gcatgacccc tggtctggca atctcgccga cggatacata
     1081 tggggccgcg gtgcgctcga catgaaagat caggtcgcgg ccgaggtcgc cgcctgcgtc
     1141 aacctggcta ggtccggatg gcggccggcc cgtggtgacc tgatcctggt cgtcgccgcc
     1201 gacgaggaga ccggcgccca tttcggggcc aagtggctct gcgaggagca cccggatgag
     1261 gtccgctcgg actgggtcct gaacgaaggc gccggcgaac tgctcgaatt cgacggtcgc
     1321 cggctcttca ccctctcgat cggcgagaag ggcactttcc gcttcaccgt gatcaccgaa
     1381 ggggaggccg gccacggttc gatcccccgg gtcggtgaca acgccctgct caagctcgcc
     1441 cccgcgcttt cgaacgccca tcgccagccg ccgcatgagt cgaccccgga caccgacctc
     1501 ttcctcgaaa ggctgctcgg ccatccggtc gaggacaccg acgccgccct gcgcgagatc
     1561 gaagcgatcg acccgctggt cgccaagctt ttggccgacc cgatgctcgg agtgacgatc
     1621 gccccgacca tggcgagcgc ttccagccgc gagaacgtga tcccctcccg ggccgagctg
     1681 gtgatcgact gccgcacccc gcccggcatg accgaagagc aggtccgggc ccggatcgac
     1741 ctcgtactcg gtgacggcga ctggcggatc gagttcaacg accccgtggt cggcaatcgc
     1801 tccgattacg acggaccgct ggccgaggcg atcgaggagt gggtcgggtt gaccgaaccg
     1861 ggggccgagg tcctgcccca ggtcatgccc ggcttctcgg attcccactg gttccgcaag
     1921 gcattcggcg ccaccgtctt cggcttcgcc ccccagcgca tgagcatcgc cgaaaccaag
     1981 cccctcgtcc acggcaaaga cgaacgaatc accgtcgagg acgtagagct gatggaaaac
     2041 ctctacacct ggatcccacc ccgcctgctc gggaccgcgg agtcctgacc cggcccaggc
     2101 cgatcacgcc ccacaagatt ttcgctgcgg gaaaccgtgg attaggacgg tttgggtcga
     2161 cggggcctgc gatcggccgg gtcgaccctc agctgccaag gtcgagattc gcgaccgaca
     2221 agctcgccag tgaggcagtg aacgagaaac aacgggcgga attcatctgt tgacggttgg
     2281 taattcattc gtctttcggt ttcgtttcct tggaaacctg atcctgtccc gaatactgag
     2341 gtggcaacgg tgatgtgggg tacggctcgc gcttgggcat cggcccaaaa agccaccggg
     2401 tgaaccggcc agcgccggag aacaagccag cgccgtagac catgaagaca atcatcggcg
     2461 cgagctcttc gagaaaagat gcgttccacg ggtagtggat gtggctcgac atcaaaaaga
     2521 ccgggacgaa cccgaggatc agaaacaatc caaatacgat ctggtggacg agccgggaca
     2581 cctcaaactt tgggccatga ttcgtgaaga ggcccttcca gccgagcctt tcgctccccc
     2641 tggtctcagt cgatccttcg actttctgct ccatggccgg aggatatccg gcctcggccg
     2701 gatggtggga taggcctcat cgctaggctt gtcgcctcat gttcactcgc atagatcaca
     2761 tcggtgtggc tgttgaggat ctcgacgccg ccatcaagct ttatgagcgg aatt
//
LOCUS       DAOQFI010000193         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18551_length_2805_cov_3.756727, whole genome shotgun sequence.
ACCESSION   DAOQFI010000193 DAOQFI010000000
VERSION     DAOQFI010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18551_length_2805_cov_3.756727"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PLE93_04345"
     CDS             complement(<1..1334)
                     /locus_tag="PLE93_04345"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNH86311.1"
                     /translation="MSFDAPAMDYAGLSPVIALTTGLVVIVLASVFDSVKRFGPFLAL
                     LTFAVTAGCLIWQWSADIDLVSGSLRLDGLAATLSILVVIAAAVSVLLAIGDPSETQA
                     GRSDWLALLTGSVLGMVILAQATSMLTFFVGLELLSIPLYALCGTNLRRRESLESGLK
                     YLIVGSVGSATLLYGLAMIYGASGATGFQAISDGLQAGDGMLSDSLTLVGVGLVAVGL
                     AFKVSIAPFHQWTPDVYQGAPTPVTAFMAVATKAAAFAVFIRFFLVALGPLADQWDVM
                     LAALAALSIVVGNVGALAQTSLKRMLGYSGIAQAGYMLAGIVTATQAGVDALVFYLAA
                     YVAMNVGAFAVVVARERQTGFSDDISTLRGLGREKPLLAWPLTISMLALAGLPPTVGF
                     IGKLYLIEALVDDDWTWLAVMIAIGTMISLGYYLRVVATVWMSPADESEAAAA"
     gene            complement(1331..>2805)
                     /locus_tag="PLE93_04350"
     CDS             complement(1331..>2805)
                     /locus_tag="PLE93_04350"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNH86312.1"
                     /translation="FGLIGLLVPRRFAAWVAALGAVITLGLSIGVLAGFDPDGGMQFV
                     TDVSWIPGLGVDYSLGIDGVSIFLVMLTAIAWIPAIGFSALRGTERPALFYLMLLAGE
                     TSTLGAFLAQDLLLFVLFFDLMIVPFYFLFGIWGRDRGGESDPEGSPGNGPVTVQSAT
                     LKMIVFTLIGSLLMLVGAIAAAVIAADGGELTFSIQTIVARGLPEGSQNWIFWFFAAA
                     FLVKMPAFPLHGWMPDSYRAAPLPALAVFSAVLSKVGAYGFLRVVLPMMPDAADLFQT
                     TMLVLAIASILYGSAMAFTKTDLRLVLGFSSVAQLGFITAGIFAFNESGAEGAVLQMV
                     NHGLVVVPAFLIVAMISERTGSEELGPMGGLAKKAPVFAVIFLIVSMATLAIPASANF
                     IGEFFILNGLFQVDAAWAIVASVGVALAAFYALRMYQLTMHNPLPDGSDSKEISLRDA
                     LVVIPMVAIVVVLAFCPQLIIHGSEPAVDSSIQVVKEVAE"
BASE COUNT          593 a          889 c          978 g          345 t
ORIGIN      
        1 gctgcggcgg cctcggactc gtccgccggg ctcatccaga cggtggcgac cacccgcagg
       61 tagtagccga gcgagatcat cgtgccgatc gcgatcatca ccgccagcca ggtccagtcg
      121 tcatcgacca gggcctcgat caggtacagc ttgccaatga agccgacggt cggcggcagg
      181 ccggcgaggg ccagcatcga gatcgtcaaa ggccaggcga gcaggggctt ttcgcgaccg
      241 agaccgcgca gggtcgagat gtcatcggag aagccggtct gacgctcgcg ggccacgacc
      301 accgcgaagg caccgacgtt catcgcgaca taggcggcga ggtagaagac cagggcgtcc
      361 accccggcct gggtcgcggt gacgatcccg gccagcatgt acccggcctg ggcgatgccg
      421 gaatagccga gcatgcgctt cagcgaagtc tgggcgaggg cgccgacgtt gccgaccacg
      481 atcgagagcg cggccagggc ggccagcatc acgtcccact gatcggccag cggcccgagt
      541 gcgaccagga agaagcggat gaagacggcg aaggcggcgg ccttggtcgc aaccgccatg
      601 aaggccgtaa ccggggtcgg ggcgccctgg tagacatccg gcgtccactg atggaagggc
      661 gcgatcgaaa ccttgaaggc gaggccgacc gcgaccaggc cgactccgac cagggtcaga
      721 gagtccgaaa gcatgccgtc gccggcctgc agcccgtcac tgatcgcctg gaagccggtc
      781 gcgccggagg cgccgtagat catcgccagg ccgtaaagca gggtggccga accgaccgaa
      841 ccgacgatca ggtacttgag cccggactcg agcgattcgc gacggcgcag attggtgccg
      901 cagagcgcgt aaagcgggat cgagagcagc tcgaggccga cgaagaaggt caacatgctg
      961 gtcgcctgcg cgaggatgac catgccgagt accgatccgg tcaaaagcgc cagccagtcg
     1021 cttcgcccgg cctgggtctc ggagggatca ccgatcgcca gcagcaccga gaccgccgcc
     1081 gcaatcacga cgaggatcga aagggtcgcg gccagaccgt ccagcctgag cgaacccgag
     1141 accaggtcga tatcggcgct ccattgccag atcaggcagc cggccgtgac cgcgaaggtc
     1201 agcagggcaa ggaacggacc gaagcgcttg accgagtcga atacggaagc cagcacgatc
     1261 acgaccaggc cggtggtcag agcaatgacc ggcgagaggc cggcgtagtc catggcgggt
     1321 gcgtcgaagc tcactccgcc acctccttca caacctggat cgaagagtcc accgccggct
     1381 cggaaccgtg gatgatcagc tgcgggcaga aggccagcac gaccacgatg gcgaccatcg
     1441 ggatcaccac cagggcatcc cgcagcgaga tctccttcga gtcggagccg tcgggcagcg
     1501 ggttgtgcat ggtcagctgg tacatccgga gcgcatagaa ggcggccagg gcgacaccga
     1561 ccgaggcgac gatggcccag gccgcgtcaa cctggaagag cccgttgagg atgaagaact
     1621 cgccgatgaa gttggccgag gcggggatcg ccagggttgc catcgagacg atcaggaaga
     1681 tcaccgcgaa gaccggcgcc ttcttggcca gtccgcccat cgggccaagc tcctcggagc
     1741 cggtccgttc actgatcatc gccacgatca ggaaggcggg gaccacgacc agtccgtggt
     1801 tgaccatctg cagcaccgcg ccctccgcac cggattcatt gaaggcgaat atgccggccg
     1861 tgatgaatcc cagctgggcg accgaagaga aaccgagcac gagccgcagg tcggtcttgg
     1921 tgaaggccat cgccgagccg tagaggatgg aggcgatcgc gaggaccagc atcgtcgtct
     1981 ggaagaggtc ggcagcgtcg ggcatcatcg gcagcacgac cctgaggaag ccgtaagcgc
     2041 ccaccttcga gagcacggcg gagaagaccg ccagggccgg cagcggcgcc gcccggtagg
     2101 agtcgggcat ccagccgtgg agcgggaagg ccggcatctt gaccaggaag gcggcagcga
     2161 agaaccagaa gatccagttc tgggaaccct cgggcaggcc gcgggcgacg atcgtctgga
     2221 tcgagaaagt cagctctccc ccgtcggccg cgatcaccgc ggcggcaatc gctccgacca
     2281 gcatcagcag cgagccgatc agggtgaaga cgatcatctt cagggtcgct gactgcaccg
     2341 tgaccggtcc gtttccggga cttccttccg ggtcgctttc gccaccccgg tcgcggcccc
     2401 agatcccgaa gaggaagtag aagggcacga tcatcaggtc gaagaagagg acgaagagca
     2461 gcaggtcctg ggcgaggaag gcgccgaggg tcgaggtttc gccggccagc agcatcaggt
     2521 agaagagggc cggccgttcg gtcccccgca gggccgagaa accgatcgcg gggatccagg
     2581 cgatcgcggt cagcatgacc aggaagatcg agaccccgtc gatgccgagg ctgtaatcga
     2641 cgccgaggcc ggggatccag gagacgtcgg tgacgaactg catgccgcca tcggggtcga
     2701 agccggccag cactccgatc gagaggccga gcgtgatcac cgcgccgagc gccgcgaccc
     2761 aggcggcgaa acgccggggg accagcagcc cgatcaggcc gaaga
//
LOCUS       DAOQFI010000194         2801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18603_length_2801_cov_3.049527, whole genome shotgun sequence.
ACCESSION   DAOQFI010000194 DAOQFI010000000
VERSION     DAOQFI010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18603_length_2801_cov_3.049527"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..955)
                     /locus_tag="PLE93_04355"
     CDS             complement(35..955)
                     /locus_tag="PLE93_04355"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNH86313.1"
                     /translation="MKVALLQGGRSLERGVSLRSGMRVTESLLDLGHEVDVMDLGPDL
                     VNRLEAKRPDVVFIALHGTEGEDGTIQALLELLGIPYTGPGVAACTRCMDKSLTKYIL
                     REHDLPTPDWVTYSEASIRDFGAASTFGAAVERVGLPLVAKPASQGSSLGVKVVTELE
                     QIPGALITALSYDNRALLEAWVNGRELAVSVLGGEALPVVEAIPTDSNAYDFEARYEI
                     GGASFECPADLPDADAAEVERVALAAYESLGCSGFARVDIMLGESGPQILEINAIPGL
                     TETSLLPQAAEAAGLDFNQMVQRILDLALT"
     gene            complement(952..2310)
                     /locus_tag="PLE93_04360"
     CDS             complement(952..2310)
                     /locus_tag="PLE93_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNH86314.1"
                     /translation="MAASPPPRRQQTRAKDLERYASLFARRTRVMRSSAMRDMMEITA
                     RPEVISLAGGLPDTSTFPPEAFAAEMHRIEKTAVAEVLQYGPTEGSWLVREQIVEVMA
                     AEGTRVDALDITVTTGGQQGIDLITKTLIDPGDVIICDAPTYPGAIPTFCSYEADVRQ
                     IEMDADGMRVDLLEEMIAKLEAEGRKPKFIYSIPTFQNPGGVTLSLERRRRLVEIARE
                     KEILVVEDNPYGLLRFEGEPLPTLYSLDGGNFVVYLGTFSKILSAGLRIGWLVTPNPI
                     REKVVLGKQAADLCTSTLTQDFAGHFFQTGRWREYVAELTGIYRARRDVMISAMAEYF
                     PHGATWNKPQGGLFIWATMPDVLSSEDLLAKSLSRNVAFVPGPNAYVDGRGVSSMRLN
                     FSGVREDEIIEGIRRIGQVAREMLKLYETMTSTDEMKLPPPESTDGGGSEMKLPPRED
                     LP"
     gene            2298..2756
                     /locus_tag="PLE93_04365"
     CDS             2298..2756
                     /locus_tag="PLE93_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86315.1"
                     /translation="MRPPSFYSAGIDTFSTICLAAGIGIATGMIAGTTGLEGSARGRM
                     AILAGIIGAVLGYLGTSILDGAPVIGALVGAIAAVLSTAVVSDVIAGAQRRGGTAALA
                     VITVFAALVIAIICLLFPFFAIIPAAFLVFLAWRRRQQADRKHAGLRVLR"
BASE COUNT          456 a          904 c          934 g          507 t
ORIGIN      
        1 tagacagacg agaacgcgaa tcgcctgaag gatctcaagt caaagcgagg tccaggattc
       61 gctggaccat ctggttgaag tcgaggccgg cggcttcggc ggcctggggg aggagggagg
      121 tttcggtgag gccggggatt gcgttgatct cgaggatctg ggggccggat tcgccgagca
      181 tgatgtccac ccgggcgaag ccggaacagc ccagtgactc gtaggctgcg agggcgaccc
      241 gctcgacctc ggcggcgtcg gcgtcgggga gatcggccgg gcattcgaaa ctggctccgc
      301 cgatctcgta acgggcctcg aagtcgtagg cattgctgtc cgtcgggatc gcctcgacaa
      361 ccggcagggc ttcgccgccc agcaccgaca ccgccagttc gcggccgttg acccaggctt
      421 ccagcagtgc ccggttgtca tagctgagcg cggtgatcag ggcgcccggg atctgctcga
      481 gctcggtcac caccttgacg ccgagcgatg atccctgtga agcaggcttg gcgaccaggg
      541 gcagcccgac ccgttccacc gccgcaccga aagtggaggc ggcaccgaaa tcacggatcg
      601 acgcctcgct gtaggtgacc cagtccgggg tgggcagatc atgctcgcgc aggatgtact
      661 tggtcaggct cttgtccatg cagcgtgtgc aggcggcgac cccgggcccg gtgtagggga
      721 tgccgagcag ttccagcagg gcctggatcg tgccgtcctc gccctcggtg ccgtgcaggg
      781 cgatgaagac cacgtccgga cgctttgctt cgagccggtt gaccagatcc gggccgaggt
      841 ccatcacatc cacctcgtgg ccgaggtcga gcagggattc ggtaacccgc attccggatc
      901 gaagtgaaac gccccgctcg agcgagcggc cgccctgaag cagcgcgact ttcaaggaag
      961 gtcctctcgt ggcggaagct tcatttcgct tccgcctcca tcagtggact ccggtggcgg
     1021 aagcttcatt tcatcggttg aagtcatcgt ctcgtagagc ttcagcatct cgcgggctac
     1081 ctggccgatg cggcggatcc cctcgatgat ctcgtcttcc ctgactccgg agaagttcag
     1141 ccgcatcgag ctgaccccgc ggccgtcaac gtaggcattc ggtccgggca cgaaagcgac
     1201 attgcgcgaa agcgacttcg ccagcaggtc ctcgctgctc agcacgtcag gcatggtcgc
     1261 ccagatgaag aggcctccct gcggcttgtt ccaggtcgcg ccgtggggga aatactcggc
     1321 catggcgctg atcatcacat cgcgccgggc ccggtagatg ccggtcagct cggccacgta
     1381 ctcgcgccag cggccggtct ggaaaaaatg cccggcgaag tcctgggtca gggtggaagt
     1441 gcagagatcg gcggcctgct tgccgagcac gactttctcg cgaatcggat tgggagtgac
     1501 cagccagccg atcctgaggc cggccgagag gatcttcgag aaagtgccga gatagaccac
     1561 gaagttgccg ccgtcgaggc tgtagagcgt gggcaggggt tcgccctcga aacggagcag
     1621 tccgtagggg ttgtcttcga cgaccaggat ctccttttcg cgggcgatct cgaccagccg
     1681 ccggcgccgt tccaacgaga gcgtcacccc gcccggattc tgaaaagtgg gaatcgagta
     1741 gatgaacttc ggcttgcggc cctcggcctc gagcttcgcg atcatctcct cgagcaggtc
     1801 gacccgcatc ccgtcggcgt ccatttcaat ctgccggaca tcggcttcgt aggagcagaa
     1861 ggtcgggatc gccccggggt aggtcggggc gtcgcagatg atcacgtcgc cggggtcgat
     1921 cagggtcttg gtgatcaggt cgatcccctg ctggcccccg gtggtgacgg tgatgtccag
     1981 cgcgtcgacc cgggttccct ctgccgccat cacctcgacg atctgctcgc ggaccagcca
     2041 ggatccttcg gtcggcccgt actggagcac ctcggcgacc gcggtcttct cgatccggtg
     2101 catttcggcg gcgaaggcct ccggggggaa ggtggaagtg tccggcaggc cgccggcgag
     2161 ggaaatcacc tcgggacggg cggtgatctc catcatgtcc cgcatcgccg aagagcgcat
     2221 cacccgggtt cgcctggcga aaagagaggc gtaccgctca aggtctttgg cccgggtctg
     2281 ctgacgccgg ggaggtggtg aggccgccat ccttctattc tgccggtatc gacactttct
     2341 cgacaatctg cctcgccgcc ggaatcggca ttgcgaccgg aatgatcgcc ggcacaaccg
     2401 gcctggaagg ttccgcccgg ggccgcatgg cgatcctcgc cgggatcatc ggcgcggtcc
     2461 tcggctacct cggtacctcg attctggacg gcgccccggt catcggtgcc ctggtcggcg
     2521 cgattgcggc ggtgctgagc accgctgtcg tttccgacgt gatcgccggg gcccagcggc
     2581 ggggcggcac ggcagccctg gcagtgatca ccgtcttcgc ggccctggtg atcgcgatca
     2641 tctgcctgct cttcccgttc ttcgccatca tcccggcagc gttcctcgtc ttcctcgcct
     2701 ggcgccgacg ccagcaggcc gaccgcaagc acgccggtct ccgcgtcctc cgctagccgc
     2761 ccggttccgc catgggaaaa aagctggtcc tcgcctatgt c
//
LOCUS       DAOQFI010000195         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18748_length_2790_cov_3.441682, whole genome shotgun sequence.
ACCESSION   DAOQFI010000195 DAOQFI010000000
VERSION     DAOQFI010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18748_length_2790_cov_3.441682"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PLE93_04370"
     CDS             complement(<1..1399)
                     /locus_tag="PLE93_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNH86316.1"
                     /translation="MTTAIVNGISSSIATTVGLIPARGGVGNRFTAFQRTGAGNFTTM
                     TPGAGHGHFPRIGSYGFLSDCHTCALVAPDGSVEWFCAPRFDSPSLFGSILDRSAGHF
                     RFGPWDENAPLSRRYEPGTLVMETTWCTETGWVVVRDALSISSWAEQGGPRELMTGHE
                     TDRCLVRLVKCIDGEVDMEMVCRPRFNYGTEAAVFSDQGLGQAIAEGAGERLAIATDL
                     NLTLEQGQATGRLKMKHGDSAFVCLSWGEGIGPHPRSYPEATERIESSRDLWRDWLAQ
                     GTFPDHPWRIHLQRSALVLKGLTYTPSGAMLAAATTSLPETPGGERNWDYRYSWIRDS
                     TFALWSMHSLGFEQEARDFMRFVRDRAMESGGDLQIMYGIGGEHDLTEHTLDHLSGYG
                     GAKPVRTGNGAFDQRQNDVWGALLDSIYVHRKALGPGAINDETRDLVRRLVENALEAW
                     PKPDQGIWEARGEPRH"
     gene            1362..2279
                     /locus_tag="PLE93_04375"
     CDS             1362..2279
                     /locus_tag="PLE93_04375"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNH86317.1"
                     /translation="MLELIPFTIAVVIYWHRAMTLSWDGRKVPLWRQISFGFGLFLAA
                     SMLFNPWGYLAEELVIAHMVEHLLIGDIASLFIVLGLTRSLLQPVLAIPFFNRLQVLT
                     SPVVAFPLWAINMFFWHIPAMYDGAYGGAALHGVEHASFLLFGCLMWMPVFGPLPTPK
                     WFGPGWKVVYVIIVRFTAAILGNVLMWTQVTLYHNYAAGQAKWGLTAVQDQSTAGVIM
                     MVEGTFLILGVLAWTFFEAAKQSMQKQELIDLAFDRGVDIDEERIERAVKAGHGDLLE
                     KRIRAGGNGAAAGEEAMLVGDPGPNADGR"
     gene            2263..>2790
                     /locus_tag="PLE93_04380"
     CDS             2263..>2790
                     /locus_tag="PLE93_04380"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNH86318.1"
                     /translation="MPMAASPPPGTSGRFLGLLVFTTGACTMGAEIAGARLLAPYFGD
                     STIVWANTIAVVLVALSIGYWIGGRLADRHPDLRSLCGTVLCGAILVTLIPFFARPFL
                     GASVDAFDELSVGVFAGSLAGVLVLLAIPVMIFGAVSPWALRIAMDNSDVEHAGQLAG
                     RLYALSTAGSLVGTLV"
BASE COUNT          421 a          918 c          923 g          528 t
ORIGIN      
        1 agtgcctggg ttcgccgcgg gcttcccaga tgccctgatc cggttttggc caggcctcga
       61 gcgcgttctc gaccagccgc ctgaccaggt cccgggtctc gtcattgatc gctccgggcc
      121 cgagcgcctt gcgatgaacg tagatcgaat cgagcagggc gccccagaca tcgttctgac
      181 gctggtcgaa ggcgccattt ccggtccgga ccggcttcgc accgccgtat ccggacagat
      241 ggtcgagcgt gtgctcggtc aggtcgtgct cgccgccgat cccgtacatg atctgcaggt
      301 cgcctccgga ctccatcgcc cggtcgcgca cgaatcgcat gaagtcccgg gcttcctgtt
      361 cgaatcccag cgagtgcatc gaccagagcg cgaaggtgga atcacggatc caggagtagc
      421 ggtagtccca gttccgttcg ccgccggggg tttccggcag tgaagtggtg gccgccgcca
      481 gcatcgcgcc ggacggggtg taggtgagtc ccttcagaac cagggcagaa cgctggaggt
      541 ggatccgcca gggatggtcg ggaaaggtgc cctgggcaag ccagtcccgc cagaggtcgc
      601 gtgaggactc gatcctttcc gtggcttccg ggtagctgcg cggatggggg ccgatgccct
      661 cgccccagct gaggcagacg aaggcgctgt cgccatgctt catcttcagc cgtccggtcg
      721 cctggccctg ctccagggtc aggttgaggt cggtggcgat cgcaagccgc tccccggcac
      781 cttcggcgat cgcctgacca agtccctggt cggagaagac ggcagcctcg gtgccgtagt
      841 tgaaccgcgg tcggcagacc atctccatgt cgacttcacc gtcgatgcac ttgaccagcc
      901 gaaccaggca gcggtcggtc tcgtggccgg tcatcagctc ccgtggcccg ccctgctcgg
      961 cccaggacga gatcgagagg gcgtcgcgga ccacgaccca gccggtctcg gtgcaccagg
     1021 tggtctccat gaccagggtg cccggctcgt agcgccggct gagcggagca ttttcgtccc
     1081 acggtccgaa ccggaaatgg ccggcggacc ggtcgaggat cgatccgaag aggctgggcg
     1141 agtcaaaacg cggggcgcag aaccattcga ccgagccgtc cggcgccacg agggcgcagg
     1201 tgtggcaatc ggagagaaag ccatagcttc caattctcgg aaagtgaccg tggccggcac
     1261 cgggagtcat cgtggtgaaa ttaccagcgc cggtacgctg aaaggccgtg aatctgttgc
     1321 cgacacctcc ccttgccggg atcagcccga cggtcgtggc aatgctggag ctgatcccgt
     1381 tcacgatcgc ggtggtcatt tactggcacc gggcgatgac cctttcctgg gacgggcgta
     1441 aggtcccgct ctggcgccag atctccttcg gcttcgggct cttcctcgcc gcctcgatgc
     1501 tgttcaaccc ctggggctac ctggccgagg aactggtcat cgcccacatg gtcgagcacc
     1561 tgctgatcgg tgacatcgcc agcctcttca tcgtgctcgg cctgacccgt tccctgctcc
     1621 agccggtgct tgcgatcccc ttcttcaacc ggctgcaggt cctgaccagt ccggtggtcg
     1681 cctttccgct ctgggcgatc aacatgttct tctggcacat cccggcgatg tatgacgggg
     1741 cctacggcgg agcggcgctc cacggtgtcg aacatgcctc gtttctgctc ttcggatgcc
     1801 tgatgtggat gcccgtcttc gggcccctgc cgaccccgaa gtggttcggc cccggctgga
     1861 aggtcgtcta cgtgatcatc gtcaggttca ccgcggcgat cctcggcaac gtgctgatgt
     1921 ggacccaggt cacgctctac cacaactacg ccgccggtca ggccaaatgg ggcctcaccg
     1981 cggtccagga ccagagcacg gccggcgtga tcatgatggt cgaagggacc ttcctcatcc
     2041 tcggggtcct cgcctggacc ttcttcgagg cggcgaagca gtcgatgcag aagcaggaac
     2101 tgatcgacct cgccttcgac cgcggtgtgg acatcgacga ggagcggatc gaaagggcgg
     2161 tgaaagccgg ccacggagac ctgctcgaga agcggatccg ggccggcggg aacggcgcgg
     2221 cggcaggcga agaggcgatg ctggtcgggg acccgggtcc gaatgccgat ggccgctagc
     2281 ccgccgccgg ggacttccgg ccggttcctc ggcctgctcg tcttcaccac cggcgcctgc
     2341 acgatgggtg ccgagatcgc cggagcccgg ctgctcgcgc cctacttcgg tgattcgacc
     2401 atcgtctggg cgaacacgat cgcggttgtc ctggtcgcac tttcgatcgg ctactggatc
     2461 gggggcaggc tggccgaccg tcacccggat ctcaggtcgc tttgcgggac ggtgctctgc
     2521 ggggcgattc tcgtaaccct gatcccgttc ttcgcccggc cgtttctcgg cgcctcggtc
     2581 gatgcttttg acgaactctc ggtcggcgtc ttcgccgggt cactggccgg cgttctggtc
     2641 ctgttggcaa ttccggtgat gatcttcggt gcggtctcgc cctgggcgct ccggatcgct
     2701 atggacaact cggacgtcga gcatgccggc cagctggcgg gccggctcta cgcgctttcg
     2761 accgccggct cgctggtcgg caccctggtt
//
LOCUS       DAOQFI010000196         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18782_length_2787_cov_3.252562, whole genome shotgun sequence.
ACCESSION   DAOQFI010000196 DAOQFI010000000
VERSION     DAOQFI010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18782_length_2787_cov_3.252562"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLE93_04385"
     CDS             complement(<1..902)
                     /locus_tag="PLE93_04385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNH86319.1"
                     /translation="MGLTSRARGGLAGALALTAALIAVSIVSSSTASAATIVNEGAPP
                     PKISKVFPNRSSVVSETIANPASADDYWTPERLASAVPAKMPNPSDALDIPDFASGAS
                     ASADGGDFTPGNVTAFPQRVHGKIFFSVGVNNFSCSGTLIDSSNQSVVFTAGHCVFDQ
                     ETHGFVSNLIFLPGYENGVAQLGAANGASVFTTSQWAQSGANAYDMGVVVLDQPFEKF
                     YGSRQIAFDLNPKGLNYTIYGYPSKPNPPYNGEVLQGCHSGFAGRDDGNGNITPYPVA
                     ASPCFMQQGASGGGWVVGGNYVNSV"
     gene            975..2354
                     /locus_tag="PLE93_04390"
     CDS             975..2354
                     /locus_tag="PLE93_04390"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH86320.1"
                     /translation="MAWNIVIVGGGFAGANVARHLEKVLPRQSSRLTLVNDVNFLLYT
                     PLLPEAAAGTLEPRHVVTPLREILHRTNLRLGVVDAHDREAREVTLRSHMGQTERIPY
                     DHLVLAPGSISRFLPVPGLSEHAVGFKSLADAIFLRNHLIETLEMANATDDRQLREEL
                     LTYVFVGGGYAGLEALAELQDFAADAMRDYPKARLHGMRWILVEAADRVLPEIDQELA
                     DYAVDELRGRGIDIKLGTTLDEVTETTATISTGETVPTRTVVWTAGVVPNPSLQNFGV
                     PLDERGRVIVDSSMAVEGNEGIWSLGDSASVPNPKGGVCPPTAQHAVRQAPVVAANIA
                     AAIGLGERKIFDYRGNASFVNLGRYKAVGKIGDRTFRGFKAWWMARSYHMSQIPGASR
                     KARAVIDWTASLPFSRDISEVGSIGRPGVLNPDRYLHGGSHRPVEGDLPAAGRDRAEA
                     DAGSAGETA"
     gene            complement(2377..>2787)
                     /locus_tag="PLE93_04395"
     CDS             complement(2377..>2787)
                     /locus_tag="PLE93_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86321.1"
                     /translation="QVCAWKWKRKKVVRWVIRHGRKKRIVTWRKVKVKVCREIPPPAP
                     ARLGVKAWEFGFTLSAKEVAAGDTIVELNNQGEDDHNLHVQRTDGGEELATPDISPGG
                     VERIRFTTAPGTYRLWCSLPTHAERGMDTTFTAQ"
BASE COUNT          483 a          915 c          912 g          477 t
ORIGIN      
        1 accgaattca cgtagttgcc gccgaccacc cacccgccgc cgctggcccc ctgctgcatg
       61 aagcagggtg aagccgcgac cgggtacggg gtgatgttgc cgttgccgtc gtcgcggccg
      121 gcgaagcccg aatggcagcc ctgaagcact tcgccgttgt acggcgggtt gggcttcgac
      181 gggtagccat agatcgtgta attgagaccc ttcggattca ggtcgaaggc gatctgccgg
      241 gatccgtaga acttctcgaa tggctggtcg agcacgacca ctcccatgtc gtaggcattg
      301 gccccgcttt gggcccactg cgaggtagtg aagacgctgg ccccgtttgc ggctccgagc
      361 tgggcaacac cgttttcgta cccgggcagg aaaatgagat tgctgacaaa gccatgggtc
      421 tcctggtcga agacacagtg gccggcggtg aagacgacac tctgattgga tgagtcgatc
      481 agggttcctg agcagctgaa gttgtttacg ccgaccgaaa agaagatctt gccatggaca
      541 cgctgcggaa acgcggtcac gttgccgggg gtgaagtcgc cgccatctgc ggaagcggac
      601 gctccagacg cgaaatcggg gatgtcgagc gcatccgacg gattcggcat tttggccggc
      661 acggcggaag cgagccgttc gggcgtccag tagtcgtcgg ccgaggccgg gttcgcgatg
      721 gtttccgaaa ccacgctgga tctgttcgga aagaccttcg agatcttcgg cggtggcgca
      781 ccttcgttga cgatcgtggc ggcacttgcg gtggacgaag aaacgatgga gacggcgatg
      841 agggcggcgg tcagggcgag tgccccggcc aatcctcccc ttgccctgct tgtgagaccc
      901 ataggtccat tagaacagat caaacggcct tcggttgcga ttcggcgaaa gggtccgctg
      961 cctatactcg ccgcatggcc tggaacatcg tcattgtcgg cggcggattc gccggtgcga
     1021 acgtcgcccg ccacctcgaa aaggtgctgc cccggcagtc atcccgcctg accctggtca
     1081 acgacgtcaa cttccttctc tacacgccgc ttctgcccga agccgccgcc ggcaccctcg
     1141 agccgcgcca tgtggtgacc ccgctcaggg aaatcctgca ccggacgaac ctgaggctcg
     1201 gagtggttga cgcccatgac cgggaagccc gcgaagtgac cctgcgaagc cacatgggcc
     1261 agaccgaacg cattccctac gaccacctcg tgctggctcc cggctcgatc tcgcgcttcc
     1321 tgccggtccc cggcctgagc gagcatgcgg tcggcttcaa gagcctggcc gatgcgatct
     1381 tcctgcgcaa tcacctgatc gaaaccctcg agatggccaa cgccaccgat gaccggcaac
     1441 tgcgggaaga actccttacc tacgtcttcg tcggaggcgg atacgctggg ctggaagcgc
     1501 tggccgaact ccaggacttt gccgccgacg cgatgcgcga ctaccccaag gcacgactgc
     1561 acgggatgcg ctggatcctg gtcgaggccg ccgatcgcgt gctgcccgaa atcgaccagg
     1621 agctggccga ttacgcggtg gacgaactgc gcggccgcgg catcgacatc aagctcggca
     1681 cgaccctcga cgaggtcacg gaaaccacgg cgaccatctc gaccggtgag accgtcccga
     1741 cccggaccgt ggtctggacc gcgggcgtcg tccccaatcc gagcctccag aacttcggcg
     1801 tgccgctcga cgagcggggc cgcgtaatcg tcgacagctc gatggcggtg gagggcaacg
     1861 agggaatctg gtcgctcggg gattccgcct cggtcccgaa cccgaagggc ggcgtctgcc
     1921 cgccgacggc ccagcacgcg gtgcgccagg cgccggtggt cgccgccaac atcgcggcgg
     1981 caatcggact gggggaacgg aagatcttcg attaccgggg caatgcgtcc tttgtcaacc
     2041 tcggccgcta caaggcggtc ggcaagatcg gcgatcgcac cttccgcggc ttcaaggcat
     2101 ggtggatggc ccgctcgtac cacatgagcc agattccggg cgcatcgagg aaggcacgag
     2161 cggtgatcga ctggaccgcc agcctgccct tctcccgcga catctccgaa gtcggctcga
     2221 tcggccggcc cggggtgctg aaccccgatc gctacctcca cggcggctcc catcgcccgg
     2281 tcgagggtga tctgccggcg gccggccggg accgggccga agccgacgcc gggtcggccg
     2341 gcgaaaccgc ctgaaccgaa gggcctggtc cggttcctac tgggcggtga acgtcgtatc
     2401 catgccgcgt tcggcgtggg tcggcagcga gcaccagagg cggtaggtcc ccggtgccgt
     2461 cgtgaaccgg atccgttcga cgccgcccgg gctgatgtcc ggggtcgcga gttcctcacc
     2521 gccgtcggtt cgctgaacgt gaaggttgtg gtcgtcttcg ccctggttgt tcagctccac
     2581 gatcgtgtca ccggcggcga cttccttggc cgagagggtg aagccgaact cccaggcttt
     2641 gaccccgagc cgggccggcg cgggcggagg aatctcccgg cagaccttca ccttgacctt
     2701 gcgccaggtg acgatgcgct tcttcctgcc gtggcgaatc acccaccgga ccaccttctt
     2761 gcgcttccac ttccaggcgc agacctg
//
LOCUS       DAOQFI010000197         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18788_length_2786_cov_3.531673, whole genome shotgun sequence.
ACCESSION   DAOQFI010000197 DAOQFI010000000
VERSION     DAOQFI010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18788_length_2786_cov_3.531673"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLE93_04400"
     CDS             <1..362
                     /locus_tag="PLE93_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86322.1"
                     /translation="LEEVFSHREDEDLNGPLPPGTRIGHIHLYVGDLDAARTFYEEIG
                     FSPHMNADGIGFADLSLGGTFPHRIAVNVWQGIGAPQAPEGTAGMRCFELDDPRGGEV
                     RELADPAGNRLIIRPAA"
     gene            472..1554
                     /locus_tag="PLE93_04405"
     CDS             472..1554
                     /locus_tag="PLE93_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNH86323.1"
                     /translation="MRSLIEDRFSSLRFASLRIIQICLAAGVAWLIATRVVGHYEPFF
                     APISVVLVLGLAIEQRGRRAYEIAFGVALGIAIADLIVLALGTGTWQLMLVIGLAMSA
                     AVLSGGGVMLVNQAAISGVLVATLPAPDLADTTHRFVDALVGGGVALAANAITPVDPV
                     RLVRRYLEPLLARLSGTLVDIADALDHESHEEIVAALERARSLDPAVREMKIAVEAGQ
                     ETISLAPTRRRKRGRVDQLAAAAEPIDLMIRNTRVLARACQRAIDLDEHIPPVVADSI
                     RDLATSVSRLDRHLGGAPVRSTAREAALRAAAKATAALEETSNLSVSVIVGQIRSAAT
                     DLLLGLGMTNDQALDQVRSAREKLGI"
     gene            1551..>2786
                     /locus_tag="PLE93_04410"
     CDS             1551..>2786
                     /locus_tag="PLE93_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNH86324.1"
                     /translation="MRLFPSIRPYRREWLRPDLMAALTVWAVLVPEALAYATIAGVSP
                     IVGLYAAPGALLIYAALGSSRKLVTGGDAATAALSAAIVGGIVAGDSSAFVQTTAALA
                     ICVGLIAVMAGVARLGFVVNFISIPVLRGFIIGLALTIIAGQIPHLFGVESSNGNFFD
                     RIWVFVTHLDQVDLPTVAIGLTSLAIILICKYRHPSAPGSLIAVGLGIAAVSLLDLGS
                     DGVAIVGEIKSGLPSVALPNVGLQDLGDLVAGAVGLMLVGFAESLAAAKAYPDEDEEL
                     DADRELIGTGAANVGAGLFGGFVVTGSFSKTSINSESGARSQMAGIVVALMVVITMLF
                     LTGLFEDLPEAALAAVVIAALIHAVDVVGLSELARIRDRGNRLNPANRPDFIAAVAAL
                     IGVTAFDILPGLFIGIVVSL"
BASE COUNT          406 a          974 c          922 g          484 t
ORIGIN      
        1 gcctcgagga ggtcttctcc caccgcgagg acgaagacct gaacgggccg ctgccgccgg
       61 gcacccggat cggtcatatc catctctacg tgggggatct cgacgcagcc cggaccttct
      121 acgaggagat cggtttcagc ccgcacatga acgccgacgg gatcggtttc gccgaccttt
      181 cgctcggcgg cacctttccc caccggattg ccgtgaacgt ctggcagggg atcggtgccc
      241 cgcaggcccc ggaaggaacg gccggcatgc gttgcttcga actcgatgat ccgcggggcg
      301 gggaagtgcg cgaactggcc gacccggccg gaaaccgcct gatcatcaga cctgcagcct
      361 gaccgtcgcc agccgccggc cggccacggc tggagcgtgc agaagacgaa aaagcagacg
      421 cagaagctct aaaccgcgtg gttccggacg acccggtgaa atagtggggc cgtgcgatcg
      481 ctgatcgaag accggttctc ctccctgcgc ttcgcctcgc tgaggatcat ccagatctgc
      541 ctggcagcgg gcgtcgcatg gctgatcgca acccgggtcg tcgggcacta cgaaccgttc
      601 ttcgcgccga tctcggtggt cctggtgctg ggcctggcga tcgaacagcg aggccggcgg
      661 gcctacgaaa tcgcattcgg ggtggccctt ggcatcgcga tcgccgacct gatcgtgctg
      721 gcgctcggga ccggcacctg gcagctgatg ctggtcatcg ggctggcgat gtcagcggca
      781 gtgctttccg gcggcggggt gatgctcgtc aaccaggcag ccatttccgg ggtgctggtt
      841 gcgaccctgc cggccccgga cctggccgac acgacccacc ggttcgttga cgccctggtc
      901 ggcggcggcg ttgcgctcgc cgcaaacgca atcaccccgg tcgatccggt tcgcctggtg
      961 cgccgctacc tggaaccgct gctcgcaagg ctttcgggga ctctggtcga catcgccgat
     1021 gcgctcgacc acgaaagtca cgaggaaatc gtcgccgccc tggagcgggc ccggtccctg
     1081 gacccggcgg tgcgagaaat gaagatcgcc gtcgaggccg gtcaggaaac gatttcactc
     1141 gccccgaccc gccgacgaaa acgggggcgc gtcgatcagc tcgcggccgc cgccgaaccg
     1201 attgacctga tgatccgtaa cacccgcgtg ctggcccggg cctgccagcg cgcgatcgac
     1261 ctcgacgaac acatcccgcc ggtggtggcc gactcgatcc gggaccttgc tacttccgtt
     1321 tcccggctgg accggcacct cggcggggcg ccggtccgct cgaccgcccg ggaagcggcc
     1381 ctgcgggcgg ccgccaaggc gaccgccgcc ctggaggaga cctcgaatct ctcggtcagc
     1441 gtgatcgtgg gccagatccg ttcggccgca accgacctgc tgctcgggct ggggatgacc
     1501 aatgaccagg cgctcgatca ggtgcgctcg gcccgggaga agctgggcat atgaggctct
     1561 ttccttcgat ccggccgtac cgccgcgagt ggctcagacc ggacctgatg gccgcgctca
     1621 cggtctgggc cgtgctggtg ccggaagccc tggcctacgc gacgatcgcc ggggtatccc
     1681 ccatcgtcgg cctctacgcg gcgcccggcg ccctcctgat ctacgcagcg ctcggcagtt
     1741 cacgcaagct ggttaccggg ggcgacgccg ccaccgcggc tctttcggcc gcgatcgtcg
     1801 gcggcatagt tgccggcgac agctcggcct tcgtccagac caccgccgcc ctcgcgatct
     1861 gcgtcggcct gatcgcggta atggccgggg tcgcccggct gggtttcgtg gtcaacttca
     1921 tttcaattcc ggttctccgg ggattcatca tcggcctggc cctgaccatc atcgccgggc
     1981 agatcccgca cctcttcggg gtcgagtcca gcaacgggaa cttcttcgac cggatctggg
     2041 tcttcgtgac ccacctcgac caggtggacc tgccgaccgt ggcgatcggg ctcacctcgc
     2101 tggcgatcat cctcatctgc aagtaccggc acccgtcggc gcccggctcc ctgatcgcgg
     2161 tcgggctggg gatcgccgcg gtcagcctgc ttgaccttgg ctccgacggc gtcgccatcg
     2221 tcggcgagat caagtccggc ctgccttcgg ttgccctgcc gaacgttggc cttcaggatc
     2281 tcggtgacct ggttgccggc gcggtcgggc tgatgctggt cggctttgcc gaaagcctcg
     2341 ccgcggccaa ggcctatccc gacgaggacg aggagctcga tgccgaccgg gagctgatcg
     2401 gcaccggcgc ggccaacgtg ggagccggcc tcttcggtgg cttcgtggtc acggggagtt
     2461 tctcgaagac ctcgatcaac tccgagtccg gggctcgcag ccagatggcg ggcatcgtcg
     2521 tcgccctgat ggtcgtgatc acgatgctct tcctcaccgg tctcttcgag gacctcccgg
     2581 aagcggccct cgccgccgtg gtgatcgccg ccctgatcca cgccgtggac gtggtcggtc
     2641 tgagtgagct cgcccggatc agggaccgcg gcaaccggct caaccccgcc aaccggcccg
     2701 acttcatcgc cgcggtcgcc gcgctgatcg gagtcacggc cttcgacatc ctgcccggac
     2761 tcttcatcgg catcgtggtt tcgctg
//
LOCUS       DAOQFI010000198         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18811_length_2784_cov_3.331623, whole genome shotgun sequence.
ACCESSION   DAOQFI010000198 DAOQFI010000000
VERSION     DAOQFI010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18811_length_2784_cov_3.331623"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLE93_04415"
     CDS             complement(<1..627)
                     /locus_tag="PLE93_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH86325.1"
                     /translation="MAVALFFGLLLAGCGLGPGEDTGDASLLVTRDYGSRVLFDNPGL
                     AVNESSNAMRLLDGNAELETRYGGEYVQSVDGLSGDRKDGRSFDWFFAVNGIVAERGS
                     AQFPVTGGDRVWWDYRDWTDAIVPEDRDRIWEAAMSKQALGTYDEEYRILRPDGEIRW
                     VRDRAFPVRDPHGNLIRIAGVAEDITARRQLESQIRESQKMESIALLAG"
     gene            complement(694..1419)
                     /locus_tag="PLE93_04420"
     CDS             complement(694..1419)
                     /locus_tag="PLE93_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86326.1"
                     /translation="MTNRITSPALGLLLGLTLLLGLAAQPAAAGNRVAANLRVVTWKG
                     QIIFDGKVKTGTAKIKPNDDCLGGHAGPARTVTGPTALGLLYQASLRYKALQPLKISD
                     GDFGFGICGIADVSAKNKEWWVLRRNNKDSMSGAETTRVKAGDTILLYLARTYEDTAP
                     DSLVLNAPTKARKGARVKVRVFSFTGAGKRTPVEGARVFGAKGALTDAQGYTYLTING
                     RTNLVARQGSLIPSNRVSVSIRK"
     regulatory      complement(1467..1650)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1735..>2784
                     /locus_tag="PLE93_04425"
     CDS             1735..>2784
                     /locus_tag="PLE93_04425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86327.1"
                     /translation="MPVAVGPSWLDPESLIRTFGMLGVLAIVYMESGLMVGFFLPGDS
                     LLFTAGLLSANDVLPDIWVLLVTIPIAAIAGDQTGYWIGRKFGPPLFNRPDSRFFKRE
                     YVDQTAAFFEKHGPRAIILARFVPIVRAFVPVMAGTSHMHYRTFLVYDIVGGILFGAI
                     TDRIGRTRTLLLTMALYSLGTAACALAPNIWVLIACRVVASLGIGGEWAAGASMVAEV
                     VPEHRRVEAGALLYTSAPLGLFLATFVDWYIAGHLLLDDPASSWRYVFLCGLIPSAVA
                     FLVRLFVKEPERWHDATKDQAPPTLADLFTPGMARITLGGLAMAVVALLTWWSCNAFI
                     AMVATGLATKHAHAVG"
BASE COUNT          408 a          979 c          875 g          522 t
ORIGIN      
        1 gccggcgagc agggcgatcg actccatctt ctggctctcg cggatctgcg attcgagctg
       61 ccggcgcgcc gtgatgtcct cggcgacgcc cgcgatgcga atcaggttgc cgtgcgggtc
      121 gcgaacgggg aacgcgcggt cgcggaccca acgaatctca ccgtcgggcc gcaggatccg
      181 gtattcctcg tcgtaggtcc cgagcgcttg cttgctcatc gccgcttccc agatccgatc
      241 gcggtcctcc ggcacgatcg cgtcggtcca gtcgcggtaa tcccaccaga cgcggtcgcc
      301 gccggtgacc gggaactggg ccgagccgcg ttcggcaacg atgccgttaa cggcgaagaa
      361 ccagtcgaaa ctgcggccgt ccttccggtc gccggagagc ccgtcgaccg actgaacgta
      421 ctcgccgccg tagcgggtct cgagttcggc gttgccgtca aggagccgca ttgcgttgct
      481 cgactcgttc accgccagac cgggattgtc gaacagcacc cgggacccgt agtcgcgggt
      541 gaccagcagg ctggcgtcac ccgtgtcctc gcctggcccg agcccgcagc cggccaggag
      601 cagaccaaaa aacagtgcaa ccgccatgcc gacggcccgg gcaggaaagc ccccggaccg
      661 tcggctttcg gttgcggact tcactcgttc cgcttacttc cggatcgaaa ccgagacgcg
      721 gttcgacggg atcagactgc cctggcgggc gaccagattc gtcctgccgt tgatggtcag
      781 gtaggtgtat ccctgcgcgt cggtcaacgc tccctttgcg ccgaagaccc tggccccctc
      841 gaccggggtc cgcttgccgg caccggtgaa ggagaagacc cggaccttga ccctggcccc
      901 tttccttgcc ttggtgggag cgttcagcac cagtgagtcc ggggccgtgt cctcgtaggt
      961 cctggcgagg tagagcagga tcgtgtcgcc ggccttgacc ctggtggtct cggcaccgga
     1021 catcgagtcc ttgttgttcc ggcggagaac ccaccactcc ttgtttttgg ccgagacatc
     1081 agcgataccg cagatgccga agccgaagtc accgtccgag atcttcagcg gctgaagcgc
     1141 cttgtagcgg agcgatgcct ggtagagcag gccaagcgcg gtcggaccgg tcacggtgcg
     1201 agccgggccg gcatggccgc cgaggcaatc gtcgttcggc ttgatcttcg cggtgccggt
     1261 cttcaccttg ccgtcgaaga tgatctgacc cttccaggta actacccgga ggttggccgc
     1321 gaccctgttt ccagccgcgg caggctgcgc agccaggcca agcagcaggg tcagcccgag
     1381 cagcaacccg agggcaggtg aggtaattcg attggtcatg cgttcctttt gtccggcggc
     1441 cctacgcgag accgcttttt cggtgcgcca tgtggcgcaa agccggcagg tcttcggact
     1501 tggacgtttg ctgggaggca gccttcccag accaatggtc cagtggcttt gtgccttcaa
     1561 ggacgtccct accgctgcgc gtcagttccg gactcggacc ggattccctg accccgctga
     1621 aggggtgcga ccgacttcgc aggcgatgat agagactcct gacgcggcgt gacctgtcaa
     1681 gatgccggat tccgcccctc gcccatggac tttctttctc atgtcataga caggttgccg
     1741 gttgccgtcg ggccgagctg gctcgacccg gaatcgctga tccgcacctt cggcatgctc
     1801 ggcgtgctgg cgatcgtcta catggaatcc gggctgatgg tcggcttctt cttgcccggc
     1861 gattccctgc tgttcacggc gggcctgctc tccgccaacg acgtgctccc ggacatctgg
     1921 gtgctcctgg tcaccattcc gatcgccgcg atcgccggtg accagaccgg ctactggatc
     1981 ggccgcaagt tcggaccgcc gctcttcaac cggcccgaca gccgcttctt caagcgcgag
     2041 tacgtcgacc agaccgcggc attcttcgaa aagcacggcc cgcgggcgat catcctcgcc
     2101 cggttcgtcc cgatcgtccg ggccttcgtg ccggtgatgg ccggcacctc ccacatgcat
     2161 taccgcacct tcctggtcta cgacatcgtc ggcggcatcc tcttcggcgc gatcaccgat
     2221 cgcatcggcc gcacgcggac cctcttgctc acgatggcgc tctactcgct cgggacggcc
     2281 gcctgcgcgc tcgcaccgaa catctgggtg ctcatcgcct gccgcgtcgt cgcgagcctc
     2341 ggcatcggcg gcgagtgggc ggccggcgcg tccatggtcg cggaggtcgt ccccgagcac
     2401 cgacgcgtgg aggccggcgc gctgctctac acctcggccc cgctcggcct cttcctcgcg
     2461 accttcgtcg attggtacat cgcgggccat ctcctcctcg acgatcccgc gtcctcgtgg
     2521 cgttacgtct tcctctgcgg cctcatcccg tccgcggtcg ccttcctcgt ccgcctcttc
     2581 gtgaaggagc ccgaacgctg gcacgacgcg acgaaggacc aggcgccgcc gaccctcgcc
     2641 gatctcttca cgcccggcat ggcccggatc accctcggcg gtctcgcgat ggccgtcgtc
     2701 gccctgctca cgtggtggag ctgcaacgcc ttcatcgcga tggtcgcgac cggcctcgcg
     2761 acgaagcacg cgcacgccgt cggc
//
LOCUS       DAOQFI010000199         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18834_length_2782_cov_3.711038, whole genome shotgun sequence.
ACCESSION   DAOQFI010000199 DAOQFI010000000
VERSION     DAOQFI010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18834_length_2782_cov_3.711038"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..>2782
                     /locus_tag="PLE93_04430"
     CDS             328..>2782
                     /locus_tag="PLE93_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNH86328.1"
                     /translation="MAFGVAAGQAQAKPSIAAQAKKICKKKRGKAKAKCVKKQVKRLK
                     AKARRERLKELNRPGVTIRTTEYGIPRITADSWRGLGYGYGYALANENICSMADIYTT
                     VRGERSKYFGPTGTWMLTGNGIQYTNLESDFSHKLVIAQNTIPKILKMKPPNGPLPQV
                     REVIDGYVKGYNAWLTKTGVNKIQDSTCKGKPWVKKITPLDAYLRFYELATMAGSGAA
                     VDGIANAAPPAETRTGAPSGSGDSNVTAADFKALNKPAAIGSNAVSLGSQATDTGKGM
                     LFGNPHFPWSGSERFFESQLTIPGKINVSGASLLGSPVIQIGHTQNMAWSHTVSTARR
                     FAFFRESLDPSDPTRYMVDGVSTPMKSNTITVDKGNGDTETRTLYSTKHGQISTSIQK
                     TPLFGWDSNYAYALFDANANSLRVVNQFFSFNTAESVAGMKKALETYQGVPWVNTIAA
                     DSKGNAMYADVSVVPNLTDARVAECNTPGLGEVAWGSSKVAVLDGANSDCDMQKAPGA
                     VVTGVLPPSQMPLAIRKDYGSNMNDSYWLSNPNAPLEGFPSIIGNEQSARSLRTRNGL
                     HQIETRLDGTDGDPGNKFSIDRVRQFTTNNRNYGAQVLIPGMVNYCESHPTIDSVDVS
                     AACTVLDQWDWTENLDSPGAYLGREVIGNLLAVSGGPFADPFSLSAPVNTPSGLDTSK
                     PGVPLALSAAVTYMNSKSIPLDASWRDYQYVTKNGEQIPIPGGFGGQGMFNVTSAVRN
                     SSTGVYDSVRHGSSFIITASLTGAKCPDVKTILTYSQAATNELSPHYADQTKLYSNSG
                     WVTDRFCVAQQKSSPGLKVT"
BASE COUNT          572 a          905 c          855 g          450 t
ORIGIN      
        1 cggcatcgcc agcagcatcg ccgccagtcc cgccacgatc aacagaagat tgaatcgctt
       61 cacctgactc cccttttccc tctcggtttt ctccccgggt cgaatgaccc tttccgaaga
      121 atagcgccga tcgggcgaca aaccgggaaa cggaagcggt ttcgggaaag cggccgtacc
      181 gtacgcggat gtatgcttgg accgctccga agctctgttt tcggggttag agggattgga
      241 gaaatgaggg gccgcagggg cgtctcgtca aagactggag agaacttgaa gtccatcaga
      301 cttttggttg cagccattgt catcttgatg gcattcggtg ttgcagcagg acaggcacag
      361 gccaagccga gcattgcggc gcaggcgaag aagatctgca agaagaagcg cggcaaggcg
      421 aaggccaagt gcgtgaagaa gcaggtcaag cggctcaagg cgaaggccag gcgggaacgg
      481 ctcaaggagc tgaaccgccc cggcgtcacg atccggacca ccgagtacgg gattccgcgc
      541 atcaccgctg acagctggcg cggcctcggt tatggctacg gctacgccct ggccaacgag
      601 aacatctgct cgatggcgga tatctacacc acggtgcgcg gcgagcgctc gaagtacttc
      661 ggcccgaccg gcacctggat gctgaccgga aacgggattc agtacacgaa cctcgagtcg
      721 gatttctcgc acaagctggt catcgcccag aacacgatcc ccaaaatcct gaagatgaag
      781 ccgccgaatg gtccgctgcc gcaggtcagg gaagtgatcg acggttacgt caagggctac
      841 aacgcgtggc tgaccaagac cggcgtcaac aagatccagg acagcacctg caagggcaag
      901 ccgtgggtga agaagatcac cccgctcgac gcctacctgc ggttctacga gctcgccacc
      961 atggccggct ccggcgccgc ggtcgacggc atcgccaatg cggccccgcc ggccgagacg
     1021 cgtaccggcg ccccgtccgg ttccggtgac tccaacgtca ccgccgccga cttcaaggcg
     1081 ctcaacaagc ccgccgcgat cggctcgaac gccgtttcgc tgggttcgca ggcaaccgat
     1141 accggcaagg gcatgctctt cggcaacccc cacttcccgt ggtccggttc cgagcggttc
     1201 ttcgaatcgc agctgaccat ccccggcaag atcaacgtct ccggcgccag cctgctcggc
     1261 agcccggtga tccagatcgg ccacacgcag aacatggcct ggagccacac ggtctcgacc
     1321 gcgcggcgct tcgccttctt ccgcgagtcg ctggacccga gtgatccgac caggtacatg
     1381 gtcgacgggg tttcgacccc gatgaagtcg aacacgatca cggttgacaa gggcaacggg
     1441 gacaccgaga cccgcaccct ctactcgacc aagcacggac agatctcgac ttcgatccag
     1501 aagaccccgc tgttcggctg ggacagcaac tacgcctacg cgctgttcga cgcgaatgcg
     1561 aacagccttc gggtcgtcaa ccagttcttc agtttcaaca ctgcggaaag cgttgccggc
     1621 atgaagaagg cgctggagac ctaccagggt gtgccgtggg tcaacacgat cgcggccgac
     1681 tccaagggca acgcgatgta cgccgacgtt tcggtcgtgc cgaacctgac cgacgcgcgg
     1741 gttgcggaat gcaacacgcc tggcctgggt gaagtcgcct ggggcagctc gaaggtcgcg
     1801 gtgctggatg gcgcgaattc ggactgcgac atgcagaagg cacccggagc cgtagtgacc
     1861 ggtgtcctgc cgccgagcca gatgccgctg gcgatccgca aggactacgg gtcgaacatg
     1921 aacgacagtt actggctgtc gaacccgaac gctccgctgg agggtttccc gagcatcatc
     1981 ggcaacgagc agtcggcccg gtcgctgagg acccggaacg gcctgcatca gatcgagacc
     2041 aggctcgacg gcaccgacgg tgacccgggc aacaagttct cgatcgaccg cgtgcgtcag
     2101 ttcaccacga acaaccggaa ctacggcgcc caggtgctga ttccgggcat ggtcaactac
     2161 tgcgagagcc acccgacgat cgactcggtc gatgtgtcgg cagcctgcac ggtgctcgac
     2221 cagtgggact ggaccgagaa cctcgattcg cccggggcct acctcggtcg cgaggtgatc
     2281 ggcaacctgc tcgcggtttc cggcggtccg ttcgcggatc cgttcagcct gagcgccccg
     2341 gtcaataccc cgagtggtct cgacacgtcc aagccgggcg tgccgctggc cctctcggct
     2401 gcggtcactt acatgaacag caagagcatc ccgctcgacg cttcctggcg cgactaccag
     2461 tacgtgacca agaacggtga gcagatcccg atccccggcg gtttcggcgg tcagggcatg
     2521 ttcaacgtga cctccgcggt gaggaactca tccaccggcg tgtacgacag tgtgcgccac
     2581 ggctcgagct tcatcatcac ggcgtcgctg accggcgcga agtgtccgga cgtgaagacg
     2641 atcctgacct actcgcaggc cgccacgaac gagctgtccc cgcattacgc ggaccagacc
     2701 aagctctact cgaacagcgg ctgggtcacc gatcgcttct gcgtcgccca gcagaagagc
     2761 tcacccggcc tcaaggtcac ca
//
LOCUS       DAOQFI010000200         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_18866_length_2779_cov_2.443465, whole genome shotgun sequence.
ACCESSION   DAOQFI010000200 DAOQFI010000000
VERSION     DAOQFI010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18866_length_2779_cov_2.443465"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLE93_04435"
     CDS             complement(<1..453)
                     /locus_tag="PLE93_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HNH86329.1"
                     /translation="MASVEQIIDGREIVICAGSGGVGKTTTSAAIALGMAARGHKVCV
                     LTIDPAKRLADSLGLKELGNEASKVNPKLLKEQGVDPENGGELWAMMLDAKETFDALV
                     ARHAEDAEARDRVLNNRIYQQISGALAGSQEYMAMEKLFELHSEGRFDL"
     gene            complement(453..1385)
                     /locus_tag="PLE93_04440"
     CDS             complement(453..1385)
                     /locus_tag="PLE93_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNH86330.1"
                     /translation="MNAFIPDFLDKRLLFVTGKGGVGKTTVAMALGIRAAMEGKRTIV
                     CEVAATENASRLFAQSEVGFREVELANDLWAISIDPDESMREYVLLQLKVKAMRDLLF
                     RSRMFTYLAAATPGLNELVTIGKIWELAQFDRKVKHGRKYDLVIVDAPATGHGISLLQ
                     TPRTFASIARVGPIHSQALQLEALITDHEQTGTVIVSLPEEMPVNETARLESELSGDI
                     GIAIDRVVMNALYPETFPKGDVKRLGKLKKSDDAEVRAAGRAGASQATRAAAQREQLA
                     RLEEMVETEVTTLPFIFKPELDLKAARQLAEEIT"
     gene            1440..2576
                     /locus_tag="PLE93_04445"
     CDS             1440..2576
                     /locus_tag="PLE93_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH86331.1"
                     /translation="MRILSRKNQEREKQGPEVETVPEVESAVPEPVAPAATATVEPNP
                     GPVEAAATPAEPATPPELAAPAEPATPAEPAAVPVEEELAEVEEAAAAEETEIRLKAA
                     DLLAPSRRDLSDHLKPVQPEQPVDMAEVISFANQKGGVAKTTSTLNLAVALSELGNRV
                     LCIDLDPQGNLTMSQGIDPDKCEKSMYDVLVDDLPISEVIAHREIDIAVASIDLAGAE
                     IAMSTKIGRERSLEKALKEVHSDYDYICIDTPPSLGLLTINALTASSKVIVPVQCEYL
                     SMRGLVQLQSTLKMIQENLNPNVHIEGILPTMFDSRTLHAREAVFILEENFGDLVFKS
                     RIRKAVKFAEAPVRGASVLKYDPESRAASYYRNLAKEVLSDGSS"
     gene            2587..>2779
                     /locus_tag="PLE93_04450"
     CDS             2587..>2779
                     /locus_tag="PLE93_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86332.1"
                     /translation="MREGPLADLFRSTSDEPSQDPPEFEPRTADDTGGPQGDPVEPAA
                     AASTPAPEPEVHVEPDPDAA"
BASE COUNT          511 a          898 c          875 g          495 t
ORIGIN      
        1 caaatcgaaa cggccctctg aatgaagttc gaagagcttt tccatcgcca tgtattcctg
       61 cgagccggcc agggctccgg agatctgctg gtagatccgg ttattcagca cccggtcgcg
      121 ggcttcggcg tcttcggcgt ggcgagcgac cagggcgtcg aaggtctcct ttgcgtcgag
      181 catcatcgcc cagagttcac cgccgttttc gggatctacg ccctgttctt tcagcagctt
      241 cgggttcacc ttgctggcct cgttgcccag ttccttcagg ccgagtgaat cggccagccg
      301 cttggccgga tcgatcgtga gcacgcagac cttgtggccg cgagccgcca ttccaagcgc
      361 gattgcggcc gatgtggtcg tcttgccgac accgcccgaa ccggcgcaga tcacgatctc
      421 gcggccatcg attatctgct cgacagaggc catcaggtga tttcctcggc cagctggcgg
      481 gccgccttga ggtcgagctc cggcttgaag atgaagggga gggtggtgac ctcggtctcg
      541 accatttcct ccagccgggc gagctgttcg cgctgggccg cggcccgggt ggcctgggag
      601 gcgccggccc tgcccgcggc acggacctcg gcgtcatcgc tctttttcag cttgcccagc
      661 cgtttcacat cgcccttcgg gaaggtttcc gggtaaagcg cgttcatcac gacacggtcg
      721 atcgcgatgc cgatgtcacc ggaaagctcc gattcgagcc gggcggtttc gtttaccggc
      781 atctcctccg gcaaggagac gatcaccgtg ccggtctgtt catggtcggt gatcagcgcc
      841 tccagctgca gggcctggct gtggatcggg ccgacccggg cgatcgaggc gaaggtgcgg
      901 ggggtctgaa ggaggctgat gccgtgcccg gtggcgggtg cgtcaacgat gaccaggtcg
      961 tatttgcggc cgtgtttgac cttgcggtcg aactgggcaa gctcccagat cttgccgatc
     1021 gtgaccaact cgttcaggcc aggggtggcg gccgcaaggt aggtaaacat gcggctgcga
     1081 aaaagcaggt ccctcattgc cttgactttc agctgcagca gcacgtactc gcgcatcgac
     1141 tcgtccgggt caatcgagat tgcccagagg tcgttggcca gttcgacttc acggaagccg
     1201 acctcggact gagcaaaaag acgggaggcg ttttcggtcg ccgcaacctc gcagacgatg
     1261 gtcctcttgc cttccatcgc ggcgcggata ccgagagcca tggcgaccgt ggttttgccc
     1321 acgccgccct ttccggtgac gaagagcagg cgtttgtcga gaaagtcggg aatgaaggca
     1381 ttcaaccgga ttgagggtgt ggggaggaaa ccggctttca catgcgaata acggcccaaa
     1441 tgcgaatctt gagccgcaag aaccaggaac gtgaaaagca gggacccgag gtcgagaccg
     1501 tgccggaagt cgagtcggct gttccggaac cggtcgcacc cgcggcgacg gccacagttg
     1561 agccgaaccc cgggccggtc gaagcggctg ctactcctgc cgagccggcc actccccctg
     1621 agttggccgc ccctgccgag cctgccactc ccgccgagcc agccgccgtg ccggtcgagg
     1681 aggaactggc tgaggtcgag gaagccgcgg ctgccgaaga gaccgagatc cggctcaagg
     1741 ccgccgatct gctcgccccg agccgccgcg acctgagcga ccacctgaag ccggttcagc
     1801 cggaacagcc ggtcgacatg gccgaggtga tcagcttcgc caaccagaag ggtggcgtcg
     1861 ccaagacgac ctccaccctg aacctggcgg tggccctctc cgagctcggc aaccgggtcc
     1921 tctgcatcga ccttgacccg cagggcaacc tgaccatgag ccagggaatc gacccggaca
     1981 aatgcgaaaa gagcatgtac gacgtgctgg tcgacgacct gccgatctcc gaagtgatcg
     2041 cccaccggga gatcgacatc gctgtcgcct cgatcgacct ggccggcgcc gagatcgcca
     2101 tgagcaccaa gatcgggcgg gaacgttcgc tcgagaaggc gctcaaggaa gtccactcgg
     2161 actacgacta catctgcatc gataccccac cgagccttgg tctcctgacc atcaacgcgc
     2221 tgacggcttc gagcaaggtg atcgtcccgg ttcagtgcga gtacctctcg atgcgcggcc
     2281 tcgtccagct tcagagcacc ctgaagatga ttcaggagaa cctgaacccg aacgtccaca
     2341 tcgaaggcat cctgccgacc atgttcgact cccgcaccct tcacgcgcgg gaagccgtgt
     2401 tcatcctcga ggagaatttc ggggacctcg tcttcaagtc ccgaatccgc aaggccgtga
     2461 agttcgccga ggcgccggta cgaggcgcta gtgtcctgaa gtacgatccc gagagtcgtg
     2521 cggcgtcgta ctaccggaat ctggcgaagg aggtcttgag tgatggctcg tcgtaagcga
     2581 gcgagtatgc gtgaaggtcc gctggcggac ctgttccgct ctacctccga cgaaccctcc
     2641 caggatccac ccgaattcga acctcgtacc gccgacgaca ccggcggacc gcagggcgac
     2701 ccggttgagc ccgcagccgc agcctcgacg cccgcacctg agcccgaggt tcacgtcgag
     2761 cccgatcccg acgccgccg
//
LOCUS       DAOQFI010000201         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19069_length_2762_cov_3.425933, whole genome shotgun sequence.
ACCESSION   DAOQFI010000201 DAOQFI010000000
VERSION     DAOQFI010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19069_length_2762_cov_3.425933"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLE93_04455"
     CDS             <1..625
                     /locus_tag="PLE93_04455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH86333.1"
                     /translation="IRPTDKHLLADGFAALSPESKFQRFFAPVVRLSESDLVYLTEID
                     HHHHEALVAIDPDEGDLIGVTRYIRTSPYRDREAEVAIVISDQWRGKGLGKALLSRLA
                     TRAREEGVTHFLAVILAENEGSIDLFENFAPKKTHVAAGDPGQVEVRIDLPWSGEFSD
                     SVLGYALRSAARGSHRFRTWRRMRRRMRRRPGREKPAGKVREFGKTD"
     gene            645..1124
                     /locus_tag="PLE93_04460"
     CDS             645..1124
                     /locus_tag="PLE93_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNH86334.1"
                     /translation="MLFSTKAEYGVRLMVELGRQPNTGPVSLNAIAEAERLPLSYLEH
                     LVAKLRQAGLVTSTRGAHGGYSLAYPAEEITMVEVVEALEGQIAPMECFHETPEGKVL
                     CSHEADSDRACATKLLWTRVQGGVTKALAGTTLAELVEFSRPAEERSKSKTAVGSAA"
     gene            1158..1922
                     /gene="sufC"
                     /locus_tag="PLE93_04465"
     CDS             1158..1922
                     /gene="sufC"
                     /locus_tag="PLE93_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNH86335.1"
                     /translation="MAELEIKNLHVSVEEKEILNGLDLTVEKGRVHALMGPNGSGKST
                     LANVIMGHPALEVTEGSIVFDGEDITDADPDERAQLGLFMAFQYPVSIPGVAVSKYLR
                     MILNAQREARGEQPLKIKEFAAQAREAMDLANIPQDFSSRYLNDGFSGGEKKRMELLQ
                     LALLKPQIAILDETDSGLDIDALRTVAEGINKFAGPDMGALVITHYQRLLHMVEPDVV
                     HVMYEGRIVKEGGKELVDQLEAEGYGWIKEEVEAAA"
     gene            1922..2332
                     /locus_tag="PLE93_04470"
     CDS             1922..2332
                     /locus_tag="PLE93_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH86336.1"
                     /translation="MAADSATPAATPTPVRSVDEIRAQFPILAREVKGKPLAYLDNGA
                     TAQKPLAVIEAMDAYYRGHNANVHRGVHTLSEEATDLYEGARAKVAGLLGSTSPREVV
                     FTRNVTSALNLARGGAPAGHPVRGRCRCAVPQPR"
     gene            complement(2253..>2762)
                     /locus_tag="PLE93_04475"
     CDS             complement(2253..>2762)
                     /locus_tag="PLE93_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNH86337.1"
                     /translation="DDITQLIAAQRAAAWGEVAQRLAHEIKNPLTPIQLSAERLQLKL
                     ADQLSGPGRELLDRAVNTIVNQVEAMKNMVNDFRDYARTPLPQLAAVDLSALLAEILG
                     LYESTRAAIVVAPAPSLPRVLADANQMRQVLHNVLSNAEDALTEVAAPRIDIVLAQDG
                     PRARLPARG"
BASE COUNT          499 a          943 c          884 g          436 t
ORIGIN      
        1 gatccgcccg accgacaagc acctgctggc cgacggcttt gccgcactca gcccggaatc
       61 gaagttccag cggttcttcg cgccggtcgt tcgcctgagt gaatcggacc tcgtgtacct
      121 gaccgagatc gaccatcacc accacgaggc gctggtggcg atcgaccccg acgaaggaga
      181 cctgatcggc gtgacccgct acatccgcac ctccccctac cgggaccgcg aggccgaggt
      241 ggcgatcgtg atttcggacc agtggcgcgg caaaggtctc ggcaaggcgt tgctttcccg
      301 gctcgccacc agggcccggg aggagggcgt gacccacttc ctggctgtga tcctcgccga
      361 aaacgagggc tcgatcgacc tcttcgagaa cttcgccccg aagaagaccc atgttgcggc
      421 tggtgatccc ggccaggtcg aggttcgcat cgacctcccc tggagtggcg aattctcgga
      481 ttcggtgctc ggttatgcct tgcgctcggc cgcccgcggg agccaccgct tccgaacctg
      541 gcggcgcatg agacgccgga tgaggcgtcg gccgggccgg gaaaagcccg ctggcaaggt
      601 gcgagaattc ggcaaaaccg actagtgctc taggatttat atcgatgctc ttctcgacca
      661 aagccgagta cggagttcga ctgatggtcg agctcggtcg ccagcccaac accggccccg
      721 tttcgcttaa cgcgatcgcc gaagccgagc gcctgcccct ttcctatctg gagcacctgg
      781 tggcgaagct tcgccaggcc ggtctggtca cttcgacccg cggcgcccac ggcggctaca
      841 gcctcgccta cccggctgag gaaatcacca tggtcgaggt ggtcgaggcc ctcgagggcc
      901 agatcgcccc gatggagtgc ttccacgaga ccccggaagg caaggtcctc tgctcgcatg
      961 aagcagacag cgaccgggcc tgcgcgacga agctgctctg gacccgggtc cagggtggcg
     1021 tgaccaaggc cctggccggc accacgctgg ccgagctggt cgagttctcg cgcccggccg
     1081 aagagcgcag caagagtaag accgcggtcg gcagcgcagc ctagccgccg ccccagactt
     1141 ttcgaacgga gcaaccagtg gcagaacttg aaatcaagaa ccttcacgtc agcgtcgagg
     1201 aaaaggaaat cctcaatggc ctcgacctga ccgtcgaaaa gggccgggtc cacgcgttga
     1261 tgggcccgaa cggttccggc aagtcgaccc tcgccaacgt gatcatgggc caccccgccc
     1321 tcgaggtcac cgagggttcg atcgtcttcg acggcgagga catcaccgac gccgacccgg
     1381 acgagcgtgc ccagctcggc ctcttcatgg ccttccagta cccggtttcg attcccggcg
     1441 tggcggtcag caagtacctg cggatgatcc tcaacgccca gcgtgaggcc cgcggcgagc
     1501 agccgctcaa gatcaaggag tttgccgccc aggcccggga agcgatggat ctcgccaaca
     1561 tcccccagga tttctccagc cgttacctca atgacggctt ctccggcggc gagaagaagc
     1621 gcatggagct gctccagctg gcgctgctga agccccagat cgcgatcctc gacgagaccg
     1681 actccgggct cgacattgac gcgctccgta ccgtcgccga gggcatcaac aagttcgccg
     1741 gccccgacat gggcgccctg gtcatcaccc actaccagcg cctgctccac atggtcgagc
     1801 cggatgtggt tcacgtgatg tacgaaggcc gcatcgtcaa ggaaggcggc aaggaactcg
     1861 ttgaccagct cgaggccgag ggctacggct ggatcaagga ggaggtcgag gcggccgcct
     1921 gatggcagcc gactccgcca cccccgctgc cacaccgacc ccggtccggt cggtcgacga
     1981 aatccgcgcg cagttcccga tcctcgcccg cgaggtaaaa ggaaagccgc tcgcgtatct
     2041 ggacaacggc gccaccgccc agaagccgct ggcggtgatc gaggcgatgg acgcctacta
     2101 ccgcgggcac aacgccaacg tccaccgcgg cgtccacacc ctctccgagg aagccaccga
     2161 cctctacgag ggcgcgcgcg caaaggtcgc cgggctgctc ggatcgacct ccccgcgcga
     2221 ggtggtcttc acccgcaatg tgacctcggc cctcaacctc gcgcggggag gcgcgcccgc
     2281 gggccatcct gtgcgaggac gatgtcgatg cgcggtgccg caacctcggt gagcgcgtcc
     2341 tcggcattgc tgagaacgtt gtgcagaacc tggcgcatct ggttggcgtc ggccagcacg
     2401 cgcggcagcg acggtgccgg cgccacgacg atcgccgcgc gcgtgctttc atacaggccg
     2461 aggatctccg cgaggagtgc cgaaaggtcg actgcggcca actggggcag cggcgtgcgc
     2521 gcatagtcgc ggaagtcatt gaccatgttc ttcatggcct cgacctgatt gacgatggta
     2581 ttcaccgcgc gatcgagcag ctcgcgcccg gggccggaaa gctgatcagc gagcttgagc
     2641 tgcagccgct cggccgacag ctggatcggc gtcagcgggt tcttgatttc atgcgccagg
     2701 cgctgcgcca cttcgcccca ggccgccgca cgctgcgcgg cgatgagctg ggtgatgtcg
     2761 tc
//
LOCUS       DAOQFI010000202         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19179_length_2753_cov_3.508895, whole genome shotgun sequence.
ACCESSION   DAOQFI010000202 DAOQFI010000000
VERSION     DAOQFI010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19179_length_2753_cov_3.508895"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="ruvA"
                     /locus_tag="PLE93_04480"
     CDS             complement(<1..543)
                     /gene="ruvA"
                     /locus_tag="PLE93_04480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNH86338.1"
                     /translation="MIATVRGEVLVRRSDYVVIEAAGVGYRLAVSSETLRSVPAAGQE
                     IFLHAETISRDDSLLLYGFSSEEERDLFNMLIGVSGIGPKVAISALSGGPHRELTRAI
                     IAGDAKRFQAVPGIGKRTAERIILELKDKVTSVAEEAGEAVLPAASDARSLARDGLIN
                     LGYAPLEAEQLLDGVGDGDDP"
     gene            complement(540..1073)
                     /gene="ruvC"
                     /locus_tag="PLE93_04485"
     CDS             complement(540..1073)
                     /gene="ruvC"
                     /locus_tag="PLE93_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNH86339.1"
                     /translation="MKVVMGIDPGTANLGFGVVRIEGGHMVALDGGVIETASGDPLER
                     RLGEIHSSLSRLIKWHQPVALAIEDLYFGKNVGSAMKVGQASGVSMLAAAQCDVPCFS
                     YTPQAIKTAVCGSGNAAKRQVQKMVATLLNLSEVPQPDHAADALAVAICHGGTAGRRD
                     ATEAALAGSGSSLRVAS"
     gene            complement(1110..1601)
                     /locus_tag="PLE93_04490"
     CDS             complement(1110..1601)
                     /locus_tag="PLE93_04490"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNH86340.1"
                     /translation="MESEGGRWSRASWAKRIGWIFGGLVALFLVIQLVPYGRDHTNPP
                     VTRSPKFVGAQTEQIFNDSCGDCHSNLTKWPWYSNVAPVSWLVQSDVDEGRSIMNFSE
                     WDKPQPAIDELVEQINEGSMPPSKYTRIHSGTKLSDSEKQTLINGLIATYRANPPGGI
                     KGG"
     gene            complement(1653..2405)
                     /locus_tag="PLE93_04495"
     CDS             complement(1653..2405)
                     /locus_tag="PLE93_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNH86341.1"
                     /translation="MSGHSKWSSIKHKKGAADAKRGQLFTKLARAITIAAREGGGDPD
                     GNPALATAIQKARAQSMPKANIEKAIDRGTGEGDDAVVVERIQFEGYGPGGAAILVDA
                     LTDNRNRTSAEVRHAFTKHNGSLGEPGSVAWMFETFGVVVVDGEANSEDDLMGAIDAG
                     ASDVVEEEGKLRVLAEPTNLTAVRQAIEADGIEIESAGLEVIPKNTVDIEESDAGTLI
                     KLLEAIEDQDDVNEVHANFDIPDEVLEKIAAD"
     gene            2434..>2753
                     /locus_tag="PLE93_04500"
     CDS             2434..>2753
                     /locus_tag="PLE93_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86342.1"
                     /translation="MIPEGKHRASRTVAAGAVAGVMLLAALSLWSVIPLVWLWVGSQF
                     ADSQFPSLGPYLIVLFGAIASILLVAWILGRLNEIYIRLTGARDVGPIRMGWTTLSSQ
                     SLRRV"
BASE COUNT          444 a          880 c          870 g          559 t
ORIGIN      
        1 cgggtcatcg ccatcgccga cgccgtcgag cagctgctcg gcttccagcg gggcataacc
       61 gagattgatc agaccgtcgc gagcgagcga gcgggcgtcc gaggcggcgg gaagaactgc
      121 ttctcccgct tcctcggcga ccgaagtcac cttgtccttg agttcgagga tgatccgctc
      181 ggcggttcgc ttgccgatcc cgggcaccgc ctgaaagcgt tttgcgtcac cggcaatgat
      241 cgcccgggtc agttcccggt gcgggcctcc cgaaagggcg gagatcgcga ccttggggcc
      301 gattccgctc accccgatca gcatgttgaa gagatcccgt tcttcctcgg atgagaagcc
      361 gtagagcagc aggctgtcgt cgcggctgat cgtctcggcg tggaggaaaa tctcctggcc
      421 ggccgccggc acgctgcgca gcgtctcgga actgaccgcc agccggtatc ccaccccggc
      481 cgcctcgatc acgacgtaat cggagcgcct gaccaggacc tctccgcgga ccgtggcgat
      541 caactcgcca cccgcaggga cgacccggat ccggccaggg cggcctcggt cgcatccctc
      601 cggccagccg taccgccatg gcagatcgct accgccaggg catcagccgc gtgatccggc
      661 tggggaactt cgctgaggtt gagcagggtc gcaaccatct tctgcacctg ccgtttggcc
      721 gcgttgcccg aaccgcagac cgcggtcttg atcgcctgcg gcgtgtagct gaaacagggc
      781 acgtcgcatt gggcggcggc cagcatcgaa acgccggagg cctgccccac cttcatcgcc
      841 gagcccacgt tcttgccgaa atagagatcc tcgatcgcca gggcaaccgg ctgatgccac
      901 ttgatcaggc gggaaaggga ggaatgaatc tcgccaagtc gccgttcgag cggatcaccg
      961 gaagcggttt cgataacccc gccgtccagg gcgaccatgt ggccaccctc gatcctgacc
     1021 acgccgaagc cgaggttcgc cgtcccggga tctatcccca ttacgacttt catccctcat
     1081 gattctgtac cgcccgtcgg atgcctcttt cagccaccct tgatgccgcc gggtggattg
     1141 gcccggtagg tggcgatcag cccgttgatc agggtctgct tttcggaatc ggagagcttc
     1201 gttccgctgt ggatcctcgt gtacttggaa ggcggcatcg aaccttcatt gatctgctcg
     1261 accagttcgt cgatcgccgg ctgcggcttg tcccactcgg agaagttcat gatcgaacga
     1321 ccctcgtcaa cgtcgctctg caccagccag gagacgggcg ccacgttgga ataccagggc
     1381 cacttggtca ggttgctgtg gcagtcgcca caggagtcat tgaagatctg ctcggtctgg
     1441 gcaccgacga acttcggtga ccgggtgacc ggaggattgg tgtggtccct gccgtagggg
     1501 accagctgga tgaccaggaa gagagcgaca agccccccga agatccagcc gattcttttc
     1561 gcccatgagg cccgactcca gcgaccgcct tcgctttcca tgggccgaag atagagcgat
     1621 cattcgatca attccagctt tgcaagagga ctctaatcgg cggcgatctt ctccagaacc
     1681 tcgtccggga tgtcgaagtt ggcgtggact tcgttcacgt cgtcctggtc ttcgatcgct
     1741 tcgagcagct tgatcagggt gccggcgtcg gattcctcga tatcgaccgt gttcttgggg
     1801 atgacttcga gcccggccga ttcgatctcg atgccgtcgg cctcgattgc ctgcctgacc
     1861 gcggtcaggt tggtcggttc ggcgaggacg cgcagcttgc cttcttcctc gaccacgtcg
     1921 ctggctccgg cgtcgatcgc ccccatcagg tcgtcttcgc tgttggcttc accgtcaacc
     1981 acgaccacac cgaaggtttc gaacatccag gcgaccgaac cgggctcgcc gaggctgccg
     2041 ttgtgtttgg tgaaggcatg cctgacttcg gcgctggtcc ggttgcggtt gtcggtcagg
     2101 gcgtcaacga ggatcgcggc accgccgggc ccgtaaccct cgaactggat ccgctcgacg
     2161 acgaccgcgt cgtcgccttc accggtgccc cggtcgatcg ccttctcgat gttggccttg
     2221 ggcatggatt gcgcccgggc cttctggatt gcggtggcga gagccgggtt gccatcggga
     2281 tcgccaccac cctcgcgggc ggcgatggtt atcgcccggg cgagtttggt gaagagctga
     2341 ccgcgctttg cgtcagctgc ccctttcttg tgcttgatcg aagaccattt cgagtgaccg
     2401 gacatgttgt gattctatga acaagagcaa ctgatgattc ccgagggcaa acacagggct
     2461 tcgaggacgg tcgcggcagg cgcggtcgcc ggggtgatgc tgcttgcggc gctttcgctc
     2521 tggtcggtga ttccgctggt ctggctgtgg gtcggatcac agttcgcgga cagccagttc
     2581 cccagcctcg ggccgtacct gatcgtgctt ttcggggcga tcgcctcgat cctgctggtc
     2641 gcctggatcc tcggccggct gaacgagatc tacatccggc tgaccggcgc ccgtgatgtc
     2701 gggccgatcc ggatgggctg gacgactttg agctcccaga gtttgcggag ggt
//
LOCUS       DAOQFI010000203         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19253_length_2746_cov_3.353029, whole genome shotgun sequence.
ACCESSION   DAOQFI010000203 DAOQFI010000000
VERSION     DAOQFI010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19253_length_2746_cov_3.353029"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..1178)
                     /gene="trpC"
                     /locus_tag="PLE93_04505"
     CDS             complement(372..1178)
                     /gene="trpC"
                     /locus_tag="PLE93_04505"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNH86343.1"
                     /translation="MGILEQLISAASQGVKARSQQVPLADLEARLGERDHDRPFQEAL
                     TRPGMSLICEYKRKSPSAGEIAPGVELPDQVKAYEAGGAAALSVLTDETHFDGRLDDL
                     AAARAACSLPILRKDFVVDQYQLYEAAAGGADAVLLIAAVLEDDRLRDFQLKAAELDL
                     DCLVEVHDEEELERAVESGAEVIGINNRNLDTGVVDIQTTYDLITDVPTGTTVVSESG
                     ISNREELVELERVGVDAALIGESLMRSENPEEMVRLLAGTGDATTEHFLP"
     gene            complement(1195..2214)
                     /gene="trpD"
                     /locus_tag="PLE93_04510"
     CDS             complement(1195..2214)
                     /gene="trpD"
                     /locus_tag="PLE93_04510"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNH86344.1"
                     /translation="MPNDILTRAIDAAASGQHLTTDHASAVLTEIMEGRSDEVQTAAF
                     LIALRTKGETVPELIGLARTMRSLAEPVEVSGDLVDTAGTGGGPSTFNVSTTAALVAA
                     GAGCPVAKHGNRSSTSLSGSADLLEALGVDIEITPEKVAASIEETGFGFMFAPRHHKA
                     MAHVVPVRKALAVRTIFNFLGPLTNPAGARRQLMGVSDRRYQESIAEALAGLGTERAL
                     VVSGDDGIDEISITGPTRIIEVVDGGTSEWFVSPGDFELERAELGDVAGGTPDENAAA
                     TRAVLEGEKGPRRDLVVLNAAAAILVGGKAGDFAAGVVAAQEAIDSGAAAAVLARLVS
                     FTGQS"
     gene            complement(2207..>2746)
                     /locus_tag="PLE93_04515"
     CDS             complement(2207..>2746)
                     /locus_tag="PLE93_04515"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNH86345.1"
                     /translation="VETVRNDVRTVDELLAEGHDRLVVSPGPCTPDDAGISLEVIPAF
                     AEAGVPVLGVCLGHQAMVQTFGGTVIQGEPIHGKSAEIDHDGKSIYDGLPHPLTVGRY
                     HSLIAGDVPDSLEVTSTFGDIVMGVRHRELPAEGVQFHPESVLTPYGKRMIATFIGVE
                     LSLEQAAAGIPDNEGKPVA"
BASE COUNT          468 a          919 c          883 g          476 t
ORIGIN      
        1 gacccccacc cgaccccacg gctacacgaa cccgggagaa acctcaacga cccccccccc
       61 gcggctacac ggacccggga gaaacctcga cgaccccacg gctacacgga cccggtgctg
      121 gtcgacgggg ttggtttgga cctccggagg cgattcgcga gagtccactt ttggccgcat
      181 ggcgcactca actccgatct cgaatcggcg gcccggatcg aaatcggagt tgggtgcgct
      241 atttgccgat aactcccgcc tcgaattccc gagactccaa cctgctcccc cctcaccagc
      301 ccctccgact tcaaggtgcc gcaggtttgc gacctctgag gtcgtaaacc tgcgtcaccg
      361 gccccgaatg ctcaggggag aaagtgctcg gtggtggcgt cgccggtgcc ggcgaggagg
      421 cggaccatct cttccgggtt ctccgagcgc atgagggact cgccgatcag ggcggcgtcg
      481 acaccgaccc tttcgagttc gaccagttcc tcgcgattgg agatcccgga ttcggaaacc
      541 accgtggttc cggtcggaac atcggtgatc aggtcgtagg tggtctggat gtcgaccact
      601 ccggtgtcga ggttgcggtt gttgattccg atcacctcgg ccccggattc aaccgcccgt
      661 tcgagctctt cctcgtcatg gacctcgacc aggcaatcga gatcgagctc ggccgctttg
      721 agctggaaat cgcgcaggcg atcgtcttcg agcacggcag cgatcagcag gaccgcgtcg
      781 gcgccgccgg ccgcggcttc gtagagctgg tactggtcga cgacgaagtc cttgcgcagg
      841 atcggcaggc tgcaggcggc gcgggcagcg gcgagatcat cgagccggcc gtcgaagtgg
      901 gtctcgtcgg tcagcacgga cagagcggcg gcgccaccgg cctcataggc cttgacctga
      961 tcgggaagct cgaccccggg ggcgatctcc ccggcgctcg gtgacttgcg cttgtactcg
     1021 cagatcagcg acatgccggg acgggtcagc gcttcctgga acgggcggtc atggtcacgc
     1081 tcgcccaacc ttgcctccag gtcagcaagg ggcacctgct ggctgcgggc cttgacgccc
     1141 tggctcgcgg ccgaaatcag ttgctcgagt attcccaatt cgcttcagag cctactagga
     1201 ctgaccggtg aaactgacca gccgggccaa aacggcagcg gccgccccgg agtcgatcgc
     1261 ttcctgtgcc gcaaccacgc cggcggcgaa atcaccggcc ttgccgccaa ccaggatcgc
     1321 cgccgccgcg ttgaggacca cgaggtcacg cctcggaccc ttttcacctt ccagcactgc
     1381 ccgcgtggcg gccgcgtttt catccggggt gccgcccgcg acatcaccga gctcggcccg
     1441 ctcaagttcg aaatcgccgg gtgagacgaa ccattcgctg gtgccgccgt caacgacctc
     1501 gatgatccgg gtggggccgg tgatcgagat ctcgtcgatg ccgtcgtcgc cggaaacgac
     1561 cagagcccgt tcggtgccga gaccggccag cgcctcggcg atcgactcct ggtagcggcg
     1621 atccgagacg cccatcaact ggcgcctggc accggcagga ttggtcagtg gtcccaggaa
     1681 gttgaagatc gttcgcaccg ccagcgcctt gcggaccggc accacatgcg ccatcgcctt
     1741 gtggtgtctg ggcgcgaaca tgaaaccgaa gccggtctcc tcgatcgagg ccgccacctt
     1801 ttccggcgtg atctcgatgt cgacgccaag cgcctcgagc aggtcagccg aaccgctgag
     1861 gctggtgctg gaacgattgc cgtgcttggc caccggacag ccggccccgg ccgcaaccag
     1921 ggcggcggtc gtggagacgt tgaaagtcga ggggcctcct ccggttccgg ccgtgtccac
     1981 cagatcaccg gaaacctcga ccggctcggc cagggaccgc atggtccggg ccagcccgat
     2041 caactcggga acagtctcgc ccttggttct gagggcgatc aggaaggcag cggtctgcac
     2101 ctcgtcggag cggccttcca tgatctcggt gagcacagcc gaggcatggt ctgtcgtcag
     2161 gtgctggcct gaagcagccg cgtcgatcgc cctggtcagg atgtcattag gcaactggtt
     2221 tcccttcgtt gtccggaatt ccggcggcgg cctgctccag cgaaagctcg actccgatga
     2281 aggtggcgat catccgcttc ccgtagggcg tcaggaccga ttcgggatga aactggaccc
     2341 cttcggccgg aagttccctg tggcggacgc ccatcacgat gtctccaaag gtcgaagtca
     2401 cctcgagcga gtcgggaaca tcgccggcga tcagcgagtg gtagcgaccg accgtgagcg
     2461 ggtggggcag cccgtcatag atcgacttgc cgtcgtgatc gatttcggcg cttttgccgt
     2521 ggatcggctc gccctggatg accgtgcccc cgaaggtctg caccatcgcc tggtggccga
     2581 ggcagacccc gagcacggga acgccggcct cggcgaaggc tggaatcact tcgagcgaga
     2641 ttccggcgtc atccggggtg cagggacccg gtgatacaac cagccggtcg tggccttcgg
     2701 ccagcagctc gtcgactgtc cggacgtcat tgcgaaccgt ttcgac
//
LOCUS       DAOQFI010000204         2742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19302_length_2742_cov_4.478973, whole genome shotgun sequence.
ACCESSION   DAOQFI010000204 DAOQFI010000000
VERSION     DAOQFI010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19302_length_2742_cov_4.478973"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLE93_04520"
     CDS             complement(<1..708)
                     /locus_tag="PLE93_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH86346.1"
                     /translation="MKPISLALHEFRFAQKVFWRNPASVFFTALLPVLFLLIFATIFG
                     GDQIEELGGVKVTTYYIPAIVTLAVISATMVNLSMNVTIAREQGLLKRGRGTPLPPWV
                     FIAGRVGNAIVISAVMLVVVTLIGKLLYDAPIPWERIPAVLVTLVVGATSFCCLGLAM
                     TVIIPSREAASAISNAITLPLYFLSGIFIPENEIPEGVLSFANHFPVRDFFEAFFSAY
                     SPKTVGAGFEWGELAVVA"
     gene            complement(705..1622)
                     /locus_tag="PLE93_04525"
     CDS             complement(705..1622)
                     /locus_tag="PLE93_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH86347.1"
                     /translation="MSSAAISIKGLRKTYDDLEAVRGVDLEVFPEEIVAVLGPNGAGK
                     TTTVEILEGYRDRDGGEVSVLGVDPAKPTPAWRDRIGIVLQSTTLEPALTVRESLQQY
                     AGYYRNPREVDEVIGLVGLEEQAGTRARKLSGGQQRRLDVGIALVGDPDLLFLDEPTT
                     GFDPSARRQAWEMVAGLRGLGKTVLLTTHYMEEAQFLADRVAIMSEGRIVALDTPENL
                     GDRRRMPTTISFSVPAGTDLAGIAGVGQTQSSSEDDTGLRTVSIGTHEPVADLNRITG
                     WAIESDLEIQHLEVRRPSLEDVYLELTDA"
     gene            complement(1650..2564)
                     /gene="rapZ"
                     /locus_tag="PLE93_04530"
     CDS             complement(1650..2564)
                     /gene="rapZ"
                     /locus_tag="PLE93_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNH86348.1"
                     /translation="MAAAEPGEDRSEDEGQREDPVEMVVITGLSGAGKSEAIAAFEDA
                     GYFCVDNLPPQMIGALGELFRLPGSGVTKAAVVSDARGGEYFEQLNQVLDDLEGDGIK
                     PKLIFLEAHEEILTDRYKETRRRHPSAPEGRITEGIEREREILAPLRARADVVIDTSG
                     ESASTLRRRISEDLIDTGSSSLMVVNLLTFGFKNGPPRDADITLDVRFLPNPHYVDDL
                     RPLTGLDQEVRDYVEAGTMAGEFYGRLLPLLEFLIPAYVAEGKSHLTIAIGCTGGRHR
                     SITVAERIWREFSTRDDVKVRVTHRDIA"
     gene            complement(2570..2728)
                     /locus_tag="PLE93_04535"
     CDS             complement(2570..2728)
                     /locus_tag="PLE93_04535"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH86349.1"
                     /translation="MRGVGPNRKRLLIRHFGSPDRLLEASREEIEAVPGLPGKVAREI
                     HDQLNRIG"
BASE COUNT          461 a          889 c          923 g          469 t
ORIGIN      
        1 ggcgaccacc gcgagttcgc cccactcgaa acccgccccg acggtcttcg ggctgtaggc
       61 ggagaagaag gcctcgaaga aatcccgcac cgggaagtgg ttggcgaatg agaggacacc
      121 ttcggggatc tcgttttcgg ggatgaagat tccggaaagg aaatagagcg gaagggtgat
      181 cgcattggag atcgccgaag cggcttcgcg cgagggaatg atcacggtca tcgccaggcc
      241 gaggcagcag aacgaagtcg cgccgacgac cagggtcacg agcacggccg gaatccgttc
      301 ccaggggatc ggcgcgtcat agagcagctt cccgatcagc gtgaccacga ccagcatcac
      361 ggccgagatc acgatcgcgt tgccgacccg gccggcgatg aagacccagg gcggcagcgg
      421 ggttccgcgg ccacgcttga gcagaccctg ctcccgggcg atcgtgacgt tcatcgacag
      481 gttgaccatg gtcgccgaga tcacggcgag ggtgacgatc gccgggatgt aataggtggt
      541 caccttgact ccccccagtt cctcgatctg gtcgccgcca aagatcgtgg cgaagatcag
      601 cagaaagagc accggcagga gcgccgtgaa gaagaccgaa gcggggtttc gccagaagac
      661 cttctgggca aagcggaact cgtgcagtgc cagggagatc ggcttcatgc gtcggtcagc
      721 tccaggtaga catcctcgag gctgggccgg cggacttcga gatgctggat ctcgaggtcg
      781 ctttcaattg cccagccggt aatccggttg aggtcggcga ccggctcgtg agtgccgata
      841 ctgaccgtgc ggaggccggt gtcgtcttcg ctgctcgact gggtctggcc gactccggcg
      901 atgcccgcca ggtccgttcc ggccggcacc gagaaggaga tcgtggtcgg catgcgacgg
      961 cggtcaccga ggttctccgg ggtgtcgagg gcgacgatcc gcccttcgct catgatcgcc
     1021 acccggtcgg cgaggaactg ggcctcctcc atgtaatggg tggtcagcag cacggtcttg
     1081 ccgaggccgc ggagcccggc caccatctcc caggcctgcc ggcgggccga cgggtcgaag
     1141 ccggtggtcg gctcgtccag aaagagcagg tcggggtcgc cgaccagggc gatgccgacg
     1201 tcgagccggc gctgctggcc gccggacagt ttgcgggcac gcgtcccggc ctgttcctcg
     1261 agaccgacca gtccgatcac ctcgtcgacc tcgcggggat tgcggtagta gccggcatat
     1321 tgctgaagcg attccctgac cgtcagggcc ggctccagcg tggtgctctg cagcacgatc
     1381 ccgatccggt cccgccaggc gggggttggt ttggcgggat ccacgccgag gacactgact
     1441 tcgcccccgt cgcggtcacg gtagccctcg aggatctcga ccgtggtcgt cttcccggcc
     1501 ccgttcgggc caagcacggc cacgatctcc tcggggaaga cctcgagatc gactccccgg
     1561 accgcctcaa gatcgtcgta ggtcttgcgc aggcccttga tgcttattgc cgcactggac
     1621 acggccaaca accctacggg gttggggaat caggcaatgt cccggtgcgt gacccggacc
     1681 ttcacgtcgt cgcgggtcga gaactcgcgc cagatccgtt ccgccacggt gatcgaccgg
     1741 tgccggcccc ccgtgcagcc gatcgcgatc gtgaggtggc tcttgccctc cgcgacatag
     1801 gccgggatca ggaactcgag caggggcagc agccggccgt agaactcgcc ggccatggtg
     1861 ccggcttcaa cgtagtcacg gacttcctgg tcgagaccgg tcagcggcct cagatcgtcc
     1921 acgtagtggg ggttcggcag aaagcggacg tcaagggtga tgtcggcatc ccgtggcggc
     1981 ccgttcttga agccgaaagt gagcaggttg accaccatca gcgaactgga ccccgtgtca
     2041 atcagatcct ccgagatccg gcgacgcagg gtcgaggccg attcgccgga agtgtcgatc
     2101 accacatcgg cccgggcccg gagcggggcc aggatctccc tttcgcgttc gatcccttcg
     2161 gtgatccggc cttccggggc cgagggatgg cggcggcggg tttccttgta ccggtcggtg
     2221 aggatctcct cgtgggcctc gaggaagatc agcttcggct tgatcccgtc gccttccagg
     2281 tcgtcgagga cctggttcag ctgctcgaag tactcgccgc cgcgggcatc cgagaccacg
     2341 gcggccttcg tcactccgct gcccggcagg cggaagagct cgccgagcgc gccaatcatc
     2401 tggggcggca ggttgtccac gcagaagtag cccgcgtcct cgaaggcggc gatcgcctcc
     2461 gacttgcctg ccccggagag accggtgatc accaccatct cgaccggatc ctctctttga
     2521 ccctcgtctt cgcttcggtc ctcgcccggc tctgcggccg ccatgggcac tatccgatcc
     2581 tgttcagttg gtcgtgaatc tcgcgggcca ccttaccggg cagcccgggc accgcttcga
     2641 tctcttcgcg gctggcctcg agcaggcggt cgggcgagcc gaaatgcctg atgagcaatc
     2701 gcttgcggtt cggtccgacc ccgcggatcc cgtccaggac cg
//
LOCUS       DAOQFI010000205         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19318_length_2741_cov_2.541325, whole genome shotgun sequence.
ACCESSION   DAOQFI010000205 DAOQFI010000000
VERSION     DAOQFI010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19318_length_2741_cov_2.541325"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1249
                     /locus_tag="PLE93_04540"
     CDS             113..1249
                     /locus_tag="PLE93_04540"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNH86350.1"
                     /translation="MSALSGVIVADFSRVLAGPLAAMTLGDLGANVIKVEPPGGDETR
                     GWVPPVDAAGRSTYFQTANRNKRSITLDLKQEEDRALAFELAMRADVLIENFRPGTMA
                     RFGLDWETVHAAHPSLVYCSITGFGSGPGAAMPGYDPLIQALSGMMSITGPPDGEASK
                     VGVALVDVIAGLNASTGILAALHDRKTMRQGQLVEINLLSTALAALANQSSAFLNTGA
                     SPSRLGNVHPSIEPFSTYATATRPIMICAGNDRQFSQLAATIGAPGLTDDPRFATNLD
                     RVENRAPLREELERRLGLDSVEYWAEALNDAGVPAGPVNDIGGGFGLAGNLELDPVTE
                     IEGTRTTSSPIRLSGSPPERRFPPPDLDQHGEEIREWLHAPREE"
     gene            1319..2443
                     /locus_tag="PLE93_04545"
     CDS             1319..2443
                     /locus_tag="PLE93_04545"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNH86351.1"
                     /translation="MNALDGLLVADFSRVLAGPLVSMTLADFGADVIKVEPPGGDETR
                     AWSPPVDAQGRATFFLAVNRNKRSVELNLKDPEDLELAKELVGRADVLVENFRPGTMD
                     RLGLGYEEMAAANPGLVYCSISGFGSKEGAALPGYDPLVQAMSGMMSVTGPPEGDPSK
                     VGVALVDVIAGLNATIGVLIALQERAKSGQGQKVESNLLSSALAALENQASAWLNTGV
                     LPVRLGNVHPSIEPFTTFDASDGPLMICVGNDRQFKALCEVLGEPELSSDPRYVDNFD
                     RVEHRVELEAELNELFSRRTVEEWVTALHEARVPAGPVNTIDRGFAVAEALGLDPIDE
                     IDGVRTPASPIRLGRTPAETRLPPPAENAHSEEIRDWLRS"
     gene            2452..>2741
                     /locus_tag="PLE93_04550"
     CDS             2452..>2741
                     /locus_tag="PLE93_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86352.1"
                     /translation="MEENEEKAPRPVAVWVGGTLAYAVLVLALGLILTGINPDSSVFP
                     PLLTALAGLFVATAINVRPASSGDADPDWNRFQVHVIVVAITSMVLSIGLVM"
BASE COUNT          460 a          917 c          920 g          444 t
ORIGIN      
        1 gccgtcggcg taccgcttgg tcagtccacg gatcgaaaga gccgagggcg gtctggcggc
       61 acgttcaccg gttggagatg tcgcggtctg gtccatgcgt caccatgcaa ccatgagcgc
      121 actctcgggc gtcatcgtgg ccgacttcag ccgggttctg gccgggccgc tggccgccat
      181 gaccctcggc gatctcggcg ccaacgtgat caaggtcgag ccgccgggtg gcgacgaaac
      241 aaggggctgg gtacccccgg ttgacgcggc cggccgctcc acctacttcc agaccgcgaa
      301 tcgcaacaag cgctcgatca ccctcgatct gaagcaggag gaagaccgcg ccctggcctt
      361 cgagctggcg atgcgggccg atgtcctgat cgagaacttc cggcccggca ccatggcccg
      421 cttcggcctc gactgggaaa ccgtccatgc cgcccatcca tcgctcgtct actgctcgat
      481 caccgggttc ggctcgggac cgggggcggc catgccgggt tacgacccgc tgatccaggc
      541 tctcagcgga atgatgtcga tcaccggacc ccccgacggt gaggcaagca aggtgggggt
      601 cgccctggtt gacgtgatcg ccggcctcaa tgcctcaacc ggaatcctcg ccgcgcttca
      661 tgaccgcaag accatgcgcc aggggcagct ggtcgagatc aaccttcttt cgaccgccct
      721 cgccgccctc gcgaaccaga gctccgcctt tctcaacacc ggtgcctcgc cctcaaggct
      781 gggcaacgtc caccccagca tcgaaccgtt ctcgacctat gccacggcca cccggccgat
      841 catgatctgc gccggaaacg accggcagtt cagccagctc gcggcgacga tcggcgcccc
      901 cggcctgacc gatgatcccc gcttcgcgac caacctcgac cgggttgaga accgggcccc
      961 cctgcgggag gagctggagc ggcggctcgg actcgattcg gtcgagtact gggcggaagc
     1021 gctgaatgac gccggggtgc cggccggtcc ggtcaatgac atcgggggcg gtttcgggct
     1081 ggccgggaac ctcgagctcg acccggtcac cgagattgaa gggacccgca cgacctcgtc
     1141 gccgatccgt ctttcgggca gtcccccgga gaggcggttt ccgccacccg acctggatca
     1201 gcacggcgag gagatccgcg aatggctcca cgcaccccgg gaagaatgac tgcgggaaaa
     1261 cgctgatcct ggcgatcgcc tctggacgcg agcggctgtg gacggcatta tgcggggcgt
     1321 gaatgctctg gacggactct tggtggcgga cttcagccgg gtgctggccg gtccgctcgt
     1381 ttcgatgacc ctggccgatt tcggtgccga cgtgatcaag gtcgagccgc ccggagggga
     1441 tgagacaagg gcctggtcgc cgccggtcga cgcccagggc agggcgacct tctttctcgc
     1501 cgtcaaccga aacaagcggt cggtcgagct caacctcaag gaccccgagg atctggaact
     1561 ggcaaaggaa ctggtcgggc gggccgacgt gctggtcgag aacttccggc ccggcacgat
     1621 ggaccggctc ggtctcggtt acgaggaaat ggccgcggcc aaccccgggc tcgtctactg
     1681 ctcgatcagc ggcttcggct cgaaggaggg ggccgccctg cccggctacg acccgctggt
     1741 ccaggcgatg agcgggatga tgtcggtgac cggtcctccc gaaggtgacc cgagcaaggt
     1801 cggggttgct ctggttgacg tgatcgcggg cctcaacgcg acgatcgggg tgctcatcgc
     1861 actgcaggaa agggcgaagt cggggcaggg ccagaaagtg gaaagcaacc tgctgagttc
     1921 ggccctggcg gcgctcgaga atcaggcgtc ggcctggctc aacaccgggg tgctgccggt
     1981 ccggctgggc aacgtccatc cgagcatcga gccgttcacc actttcgacg cgagcgacgg
     2041 tccgctgatg atctgcgtcg gcaacgaccg ccagttcaag gcgctttgcg aggtgctcgg
     2101 cgagcctgag ctgtcatcgg acccgcgcta tgtcgacaac ttcgaccggg tcgagcaccg
     2161 ggtcgagctc gaggccgaac tgaacgaact gttctcccgc cggaccgttg aagagtgggt
     2221 gaccgccctg catgaggcaa gggtcccggc cggtccggta aacacgatcg accgtggctt
     2281 cgcggtggcc gaggcgctcg ggctcgaccc gatcgacgag atcgacgggg tccggacccc
     2341 ggcctcaccg atccggctgg ggcgcacccc ggccgaaacg cgactcccgc ccccggccga
     2401 gaacgctcac tcggaggaaa tccgcgactg gcttcgaagt tgatggcagg gatggaggaa
     2461 aacgaggaga aagcaccccg gccggttgcc gtgtgggtcg gcggcaccct ggcgtacgcc
     2521 gtcctggtac tcgcactggg cctgatcctg accggcatca acccggattc atcggtcttc
     2581 ccgccgctcc tgactgccct ggccggtctg ttcgtggcca cggcgatcaa cgtgcgaccc
     2641 gcttcctcgg gtgacgcgga tccggactgg aaccgcttcc aggtccacgt gatcgtcgtc
     2701 gcgatcacca gcatggtcct cagcatcggg ctggtcatgt g
//
LOCUS       DAOQFI010000206         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19362_length_2736_cov_2.903394, whole genome shotgun sequence.
ACCESSION   DAOQFI010000206 DAOQFI010000000
VERSION     DAOQFI010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19362_length_2736_cov_2.903394"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLE93_04555"
     CDS             <1..660
                     /locus_tag="PLE93_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNH86353.1"
                     /translation="DYNARFQQSIATGTVDFDPGIGTVATDDASQWDSDPDTNYPIHY
                     PGSEYKVTFDHPGKYLFVCKVHASVRGVVTVSDTPGNPDSDFGPAPVINFDTEGPLID
                     TYFFTSNGTDPTMPFIGHKGKGIGFSFGTNEGGSASADYYRLVKRGKGKKARYVKFFQ
                     GYNEWPTHIGMNNVRFAARSSTFKPVPGKYVAYFRAEDGKSNSSDDITLNFTVFPKKK
                     R"
     gene            663..1496
                     /locus_tag="PLE93_04560"
     CDS             663..1496
                     /locus_tag="PLE93_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNH86354.1"
                     /translation="MRGGSARKLRVALVAVAAASAAFLFAAPATANNTWVSISDFQWS
                     DKNPHIDLGESVTWLWAGPDTPHSVTGQAPRDSAFDPITTTDASQWDSDAGRNEPNHW
                     PGDEYTITFDHPGTYLFACKLHSVVRGEVTVSNLPGNPDSSRGPRPKINFDFDSPVVD
                     DYFFTADGESPAPPVVGPRGRGIGFRFSVNEATTASADYYQLVTRGKGSRKHLVRVYR
                     GYGEWPAHVGINRVGFARPTANFKPAPGRYLAWFRAEDRVQNSTGDIPLRFTISPGKG
                     G"
     gene            1530..>2736
                     /locus_tag="PLE93_04565"
     CDS             1530..>2736
                     /locus_tag="PLE93_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86355.1"
                     /translation="MAGTPGLKTSSLGGLAALVAFFTVAVLAGPPARAATNEELSSCS
                     EIKQTIEQPDGSIVGMGDCRYGVSPAPRIVLVGLNPDGSDRTAFADGGAALISVEENE
                     FGLKIIGRPDGSFLVLSQKRIWSFDGDGYPVESFGNHGVLNFSAADQMNLASAFDFAL
                     DSGGNLLVAGRRPSTATPVVGKYDQTGQLVSGYGNSGVSAELDLNFDAAIAVDNSNRV
                     VMVGEDGATRLLADGSLDLSFGPENDGYADLPAGLPYSIGEVFGLTVHPDDSITAYLD
                     RTPGMYYYPAYAVSLDSDGSPAAEDALVALGDHVSAVAPFDGGVAFVRFPGRGFQHWQ
                     FEVGTTNWPGIRSVTFSPGDSLAHGVTALSDGSLLVSGNAQGPDCSDSCVFRNHLALA
                     KIDPGTGDLV"
BASE COUNT          489 a          903 c          841 g          503 t
ORIGIN      
        1 gactacaacg cccgcttcca gcagtcgatc gcgaccggca cggtcgactt cgaccccggc
       61 atcggcaccg tggcgaccga cgatgcctcc cagtgggatt cggatcccga cacgaactac
      121 ccgattcact acccggggag cgagtacaag gtcactttcg atcacccggg caagtatctc
      181 ttcgtctgca aggttcacgc ctcggtccgc ggtgtggtca cggtctcgga cacgccgggc
      241 aatccggatt cggacttcgg gcccgccccg gtgatcaatt tcgacaccga gggtccgctg
      301 atcgacacct atttcttcac ctcgaatggc accgacccga ccatgccgtt catcggtcac
      361 aagggcaagg gcatcggatt ttccttcggc accaacgagg gtggctccgc ttcagccgac
      421 tactaccggt tggtcaagcg cggcaagggc aagaaggcca ggtacgtcaa gttcttccag
      481 ggctacaacg aatggccgac ccacatcggc atgaacaacg tcaggttcgc cgcccgctcg
      541 agcaccttca aaccggtgcc gggcaagtac gtcgcctatt ttcgggccga agacggcaag
      601 tcgaactcct ctgacgacat caccctgaac ttcaccgtct tcccgaagaa gaagcgctag
      661 gagtgcgcgg aggttccgcc aggaaacttc gcgtcgcgct ggtcgcggtc gcggcggctt
      721 cggctgcctt cctgtttgcc gccccggcca cggcgaacaa cacctgggtt tcgatctccg
      781 acttccagtg gtcggacaag aacccgcaca tcgacctcgg cgaatcggtg acctggctct
      841 gggccgggcc cgacaccccg cattcggtca ccggccaggc gcccagggat tcggccttcg
      901 acccgatcac caccaccgac gccagccagt gggattcgga cgccggcagg aacgagccga
      961 accactggcc cggggacgag tacacgatca ccttcgacca tcccggcacc tacctattcg
     1021 cctgcaaact ccattcggtg gtcaggggcg aggtcactgt ctcgaacctg ccgggcaacc
     1081 cggattccag ccggggaccg aggccgaaga tcaacttcga ctttgactca cccgtggtcg
     1141 atgactactt cttcaccgcc gacggcgaga gcccggcccc gccggtggtc ggtccgaggg
     1201 gccgcggcat cggtttccgt ttctcggtga acgaggcgac caccgcctcg gccgactatt
     1261 accagctggt cacccgcggc aagggcagca ggaagcacct cgtgagggtg taccggggct
     1321 acggcgagtg gcccgcacac gtcggaatca accgggttgg tttcgccaga cccaccgcga
     1381 atttcaaacc tgccccgggc cgctacctcg cctggttccg cgccgaggac cgcgtccaga
     1441 actcaaccgg agacatcccg ctccggttca cgatttcgcc cggcaagggc ggctgaggcg
     1501 atacgctcaa gtcaaggaga gtcgggaaaa tggcaggaac gccgggactg aaaacttcat
     1561 ccctgggggg attggcggca ctggtcgcgt tctttaccgt ggcggtcctg gccggacctc
     1621 cggcgcgggc tgcgacgaac gaagaacttt cctcctgctc ggaaatcaag cagacgatcg
     1681 agcagccgga cggttcgatc gtgggcatgg gggactgccg ctacggggtt tcacctgcgc
     1741 cccggatcgt tctggtcggg ctcaatccgg acgggtcgga ccgcacggct tttgccgacg
     1801 gcggggctgc attgatctcc gtcgaggaga acgaattcgg cctgaagatc attggccgtc
     1861 cggacggaag ttttctggtt ctcagccaga aacggatctg gtcatttgac ggggacggtt
     1921 acccggtcga gagcttcggt aaccacggag tcctgaattt cagcgccgcc gaccagatga
     1981 acctcgcctc cgccttcgat ttcgcgttgg actccggcgg gaacctgctg gttgcgggca
     2041 ggcgtccttc aacagccacg ccggtggtcg gcaagtacga ccaaaccggt caacttgtct
     2101 ctggctacgg gaactctggc gtgagcgctg aactcgatct gaattttgac gcggcgatcg
     2161 cggttgacaa ctcgaatcgc gtggtcatgg tcggggagga cggggcgacc cgcctgcttg
     2221 ccgacgggag cctcgacctt tcgttcggtc ccgaaaatga tggatacgcg gacctccccg
     2281 ccgggctccc ctacagcatc ggcgaggtct tcggcctgac cgtccatccg gatgactcga
     2341 tcaccgccta cctcgaccgg acaccgggca tgtattacta ccctgcctac gccgtttcgc
     2401 tggacagcga cggatctcca gccgctgaag atgcattggt ggcgctcggc gaccatgtgt
     2461 cggcggtcgc gccgtttgac ggtggtgtcg cgttcgtgcg tttccccggg cgcgggttcc
     2521 agcactggca gttcgaggtc ggaacaacca actggccggg catccgttcc gtgacctttt
     2581 cgcccggtga ttcgcttgcg cacggggtca ccgcgctgag cgacggatcg ctgctggtgt
     2641 ctggcaacgc gcaaggcccg gactgctccg attcctgtgt cttccggaat cacctggccc
     2701 tcgccaagat cgacccgggg accggtgatc tcgtcg
//
LOCUS       DAOQFI010000207         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19364_length_2736_cov_2.695636, whole genome shotgun sequence.
ACCESSION   DAOQFI010000207 DAOQFI010000000
VERSION     DAOQFI010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19364_length_2736_cov_2.695636"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /gene="carB"
                     /locus_tag="PLE93_04570"
     CDS             complement(<1..725)
                     /gene="carB"
                     /locus_tag="PLE93_04570"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578087.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNH86356.1"
                     /translation="MPKREDIKRIMVVGSGPVVIGQAAEFDYSGVQACKVLLEEGYEV
                     VLVNSNPATIMTDPEFATRTYVEPLLPGPVAAIIEKERPDALLPTLGGQTALNLAMDL
                     SADGTLEKFGVELIGANRDAIIRGEDREVFRQVMTEAGIRIPWSITVESVEQALKELD
                     EGDASLPAIIRPAFTMGGQGGGIAETRDELIEVVSKGIAASPIGQVLVEESVIGWGEF
                     ELEVMRDRNDNVVIICSIENVDP"
     gene            complement(732..1859)
                     /gene="carA"
                     /locus_tag="PLE93_04575"
     CDS             complement(732..1859)
                     /gene="carA"
                     /locus_tag="PLE93_04575"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNH86357.1"
                     /translation="MSEATAGYVLLEDGSRFDGILCGATEGGVGEVVFNTAMTGYQEA
                     VTDPSYAGQIITFTYTMIGNYGVSAGAMESGAAHARGVIMRNARNNSDAASAEGGWLD
                     WLEANGVWAITGVNTRALVRHIRDKGAMRGGIFPASLSTGDAMARIEAEPTMAGSDFA
                     SQVSPEAPIEHDGSGPHVVALDTGIKASIVNQFLERDCRVTLLPCLTPAEEVMAREPD
                     LLFLANGPGDPAAVSAVTETVRELIGTRPMVGICLGHQLLSRAVGLETFKLPFGHRGS
                     NHPVKDLETGVIEITSQNHGFAVVGPDGKGTIENDEPVRWQTGHGEAVLSHINLYDRT
                     VEGLRLPDAKAMTVQHHPEAGPGPRDARHLFDRFLDLAKEG"
     gene            complement(1856..>2736)
                     /locus_tag="PLE93_04580"
     CDS             complement(1856..>2736)
                     /locus_tag="PLE93_04580"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNH86358.1"
                     /translation="LHQAGAVAFTDDGLPIASPRILRRALQYQAMTGGRIALHEVDPE
                     LSGKGVMNEGVVSSELGLRGVPSISESTMIGRDAAIAGYEGGAIQIQHLSAVESIEQI
                     RRAKADGVNITCEVTPHHLCLTDEAVRDLDASKHKMNPPLRTETDRQALIEALVDGTI
                     DCIATDHAPHAIHEKEVPYEAANMGVTGLETAFSSIYTELVLPGRISLELLVEKLGCG
                     GAPFDIEPFSLAEGSRANLCLVDLEAEWVVGEDGYESRSVNSWCAGEKLRGRVLVTLA
                     DGQVAFRLRSFSLGVA"
BASE COUNT          418 a          904 c          903 g          511 t
ORIGIN      
        1 atggggtcga cgttctcgat cgagcagatg atgacgacgt tgtcgttgcg gtcgcgcatc
       61 acctcgagtt cgaactcgcc ccagccgatc accgactcct cgaccagcac ctggccgatc
      121 gggctggcgg cgatgccctt cgaaacgact tcgatcagct cgtcacgggt ctcggcgatt
      181 cccccgccct ggccgcccat ggtgaaggcc ggccggatga tcgccggcag cgacgcgtca
      241 ccctcgtcga gttccttcag ggcctgctcg accgactcga cggtgatcga ccagggaatc
      301 cggattccgg cttcggtcat cacctggcgg aagacctcgc ggtcctcgcc gcggatgatc
      361 gcgtcgcggt tggccccgat cagctcgacc ccgaacttct ccagggtgcc gtcggcagag
      421 aggtccatgg cgaggttcag cgcggtctgg ccgccgaggg tgggcagcag cgcgtccggc
      481 cgttccttct cgatgatcgc cgcgaccggg ccgggcagga gcggttcgac ataggtgcgg
      541 gtcgcgaact cggggtcggt catgatcgtg gccggattcg agttgaccag caccacctcg
      601 taaccctctt caagcagcac cttgcatgcc tggaccccgg agtagtcgaa ctcggcagcc
      661 tgtccgatca cgaccggtcc ggaaccgacc accatgatcc gcttgatgtc ttcacgcttc
      721 ggcatcgcgg ttcagccttc ctttgccagg tcgaggaaac ggtcgaagag gtggcgggca
      781 tcgcgcggcc ccggcccggc ctcggggtga tgctggacgg tcatcgcctt cgcgtccggg
      841 agtcgcagcc cctcgaccgt gcggtcgtag aggttgatgt gtgaaagcac cgcctcgccg
      901 tgaccggtct gccagcgcac cggttcgtcg ttttcgatcg tgcccttgcc atcgggcccg
      961 accaccgcga aaccgtggtt ctgggaagtg atctcgatca ccccggtctc gaggtccttg
     1021 accggatggt ttgatccgcg gtggccgaag ggcagcttga aggtttcgag gccgaccgcc
     1081 cggctgagca gttggtggcc gaggcagatg ccgaccatcg gcctggttcc gatcagttcc
     1141 cgcaccgttt cggtcacggc cgataccgcg gccgggtcgc ccggtccgtt ggcgaggaag
     1201 agcaggtcgg gttcccgggc catcacctct tcggcggggg tgaggcaggg cagcagtgtg
     1261 acccggcagt ccctttcgag gaactggttg acgatcgaag ccttgatgcc ggtgtccagg
     1321 gcgaccacgt gagggccgct gccgtcatgt tcgatcggtg cttcgggact gacctgcgag
     1381 gcgaagtcgg agccggccat ggtcggttcg gcctcgatcc gggccatcgc atctccggtg
     1441 gaaaggctcg ccgggaatat gccgccgcgc atcgcgccct tgtcgcggat gtggcggacc
     1501 agcgcccggg tgttcacccc ggtgatcgcc cagacgccgt tggcctcgag ccagtcgagc
     1561 cagccgcctt cggcgctggc cgcgtccgag ttgtttctgg cgttccgcat gatcaccccg
     1621 cgagcgtggg ctgcgcccga ttccatcgcc ccggccgata ccccgtagtt gccgatcatc
     1681 gtgtaggtga aggtgatgat ctggccggcg taggaagggt cggtaaccgc ctcctggtag
     1741 ccggtcatcg ccgtgttgaa gacgacttcc cccaccccgc cttcggttgc gccgcagagg
     1801 atcccgtcaa aacggctgcc gtcttccagc agtacatatc cggctgttgc ttcgctcatg
     1861 ccacgcccag actgaaggat ctaagccgga aggcgacctg cccgtcggcc agagtcacga
     1921 gcaccctgcc gcgcagcttc tcgccggcgc accaggagtt gaccgagcgg ctttcgtagc
     1981 cgtcttcgcc gaccacccat tcggcctcga ggtcgacgag gcacaggttg gcccggctcc
     2041 cctcggccag actgaacggt tcgatgtcga agggcgcacc gccgcagccg agcttttcga
     2101 ccagtaactc gaggctgatc cgaccgggca gcaccagttc cgtgtagatc gaggaaaagg
     2161 ccgtctcgag gccggtcacg cccatgttgg cggcctcata cggcacctcc ttctcgtgaa
     2221 tcgcatgggg tgcgtgatcg gtcgcgatgc agtcgatcgt gccgtcgacc agcgcctcga
     2281 tcagcgcctg ccggtcggtc tcggtccgta gcggcgggtt catcttgtgc ttcgaggcgt
     2341 cgaggtcacg caccgcctca tcggtcaaac agaggtgatg cggggtgacc tcgcaggtga
     2401 tgttcacgcc gtcggccttg gcccggcgga tctgttcgat cgattcgacc gcggagagat
     2461 gctggatctg gatcgccccg ccttcgtaac cggcgatcgc ggcgtcccgg ccgatcatgg
     2521 tcgattcgga gatcgaggga acacccctca ggcccagttc cgaggagacg accccttcgt
     2581 tcattacgcc cttgccggag agttccggat ccacttcgtg gagggcgatc cggccgccgg
     2641 tcattgcctg gtactgaaga gcccgacgca gaatccgggg gctggcaatc ggcagtccgt
     2701 catcggtgaa ggcgaccgcg ccggcctggt gcaggc
//
LOCUS       DAOQFI010000208         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19417_length_2731_cov_2.755232, whole genome shotgun sequence.
ACCESSION   DAOQFI010000208 DAOQFI010000000
VERSION     DAOQFI010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19417_length_2731_cov_2.755232"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLE93_04585"
     CDS             <1..294
                     /locus_tag="PLE93_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86359.1"
                     /translation="AFRFPVVAFRRPGGIYFWGRNPGGKSGKAVIRFRPPNGSGSRVL
                     KRIKVGSGGVFQGFVRTRLGRNFKGSVFADSGGQASVPFSLTTVKDFYQPPFG"
     gene            347..1399
                     /locus_tag="PLE93_04590"
     CDS             347..1399
                     /locus_tag="PLE93_04590"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH86360.1"
                     /translation="MITGGAGFVGSSLAISLAESHPDRRIVALDNLYRRGSELNLDRL
                     GQAGVEFVKGDVRNPADLAPFEDIDALIECSAEPSVMSGTDGDTSYVYETNLTGAYNC
                     LELARKSGCQFIFLSTSRIYPMVGLREAALVEGETRFELAEDQPSPGWTSAGVSERMP
                     LEGARTFYGTTKLSAEFLCAEFAAQFGVPVVINRCGVIAGPWQMGKVDQGVFTHWMLA
                     HEFEKPLSYIGFGGSGKQVRDLLHVDDLVELIEEQLADPDHWAGQTFNVGGGREVSLS
                     LLEATEICGRLTGHELAIGAVPEDRDGDVPLYISDCSRLFAHTSWRPKRDAECVLADI
                     HTWIGSDRERVGAVLG"
     gene            1411..2139
                     /locus_tag="PLE93_04595"
     CDS             1411..2139
                     /locus_tag="PLE93_04595"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH86361.1"
                     /translation="MIPARDEEGSIMKTVDGVVEVMERESLEYEVLVIDDGSKDATRK
                     LVEAAGESNANIRCRPSPYSGGFGLAVRAGLEQFEGDGVAIMMADCSDDPEDLVMYHH
                     ILAEGWDCAFGSRFMPGAVVTDYPRLKLLVNRMANAFVNVLFRLGYNDTTNAFKAYRR
                     EVIETIGPLLSKHFNLTVEMPLKAVTRGHSFKVVPTSWTNRTAGEAKLAMREMGSRYL
                     FIVLYIWLEYHLSRGDYRKAEGSG"
     gene            2136..>2731
                     /locus_tag="PLE93_04600"
     CDS             2136..>2731
                     /locus_tag="PLE93_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86362.1"
                     /translation="MTSGGSTFRDWLGRTEEVNPRSWLVGLVVALLGWQVSLQAPVYG
                     LDPSWWSGLYMASHEKMRFGTEIVFTYGPLGILREPWLFYPGLLSLIPFLYKAALFTG
                     FCIALVAVLRRRIGWLFAALAALLLLLLLRQIELAVAISMLVSILIIERSPDDRVMPF
                     LAIGGGLLAGIEMLGKLSAGPVVAVVLLLGLIGAHARMR"
BASE COUNT          432 a          867 c          902 g          530 t
ORIGIN      
        1 gcattccgct tcccggtggt cgccttccgt cgtcccggcg ggatctactt ctggggccgc
       61 aaccccggtg gcaagtcagg caaggcggtg attcgcttcc ggccaccgaa cggctccggc
      121 agccgcgtcc tgaagcggat caaggtcggc agcggcggcg ttttccaggg cttcgtgcgg
      181 acccgcctcg gtcggaactt caagggctcg gtcttcgccg actccggcgg tcaggcctcg
      241 gttcccttct ctctcaccac cgtcaaggac ttctaccagc cgcccttcgg ataaaactcc
      301 ggattccatt gtgaatctgg acgcgctacc cggggggaaa tccatcttga ttacgggcgg
      361 agccggcttt gtcggttcca gccttgcgat ctcgctggct gagtcgcatc ccgacaggcg
      421 gatcgtcgcg ctggacaacc tttatcgccg cggctccgag ctgaacctcg accggctggg
      481 gcaggccggg gtcgagttcg tcaagggtga cgtgcggaac ccggccgacc tcgcaccctt
      541 cgaggacatc gacgccctga tcgaatgttc ggccgaacca tcggtcatgt cggggaccga
      601 cggtgacact tcgtatgtct atgagacgaa tctgaccggc gcctacaact gcctggagct
      661 ggcgcggaag agcggttgcc agttcatctt cctttcaacc agccggatct atccgatggt
      721 cgggctgcgt gaagcggcct tggtcgaggg ggagacccgg tttgagctgg cagaggacca
      781 gccttcaccc ggatggactt cggccggagt ctccgagcgc atgccgctgg agggtgcgcg
      841 caccttctat ggcacgacca aactctcggc cgagttcctc tgtgccgagt tcgcggccca
      901 gttcggggtg ccggtggtga tcaaccgctg cggggtgatc gccggtccct ggcagatggg
      961 caaggtcgac cagggggtgt tcacccactg gatgctggcc cacgaattcg agaagccgct
     1021 ctcctacatt ggcttcggcg gcagcggtaa gcaagtgcgg gacctgctcc acgtcgacga
     1081 cctggttgaa ctgatcgagg agcagctggc tgacccggat cactgggccg ggcagacctt
     1141 caacgtcggc gggggccggg aggtcagcct ttcgctgctc gaggcgaccg aaatctgtgg
     1201 ccgcctgacc ggtcacgagc tggcgatcgg cgccgtgccg gaagaccgcg acggtgacgt
     1261 gccgctttac atctcggatt gctcccggct cttcgcccat acctcgtggc gaccgaaacg
     1321 cgacgccgaa tgcgtgctgg ccgacatcca cacctggatc ggttcggacc gtgaacgggt
     1381 cggggcggtg ctgggatgag gctctcggtc gtgatccccg cccgggacga agaaggctcg
     1441 atcatgaaga cggtcgatgg cgtggtcgag gtgatggagc gcgaatcact cgagtacgaa
     1501 gtgctggtga tcgacgacgg cagcaaggac gcgacccgca agctggtcga ggcggccggc
     1561 gagagcaatg ccaacatccg ctgccgccct tcgccctatt ccggcgggtt cggactggcg
     1621 gtgcgggccg gcctcgagca gttcgagggt gacggggtgg cgatcatgat ggccgactgt
     1681 tccgatgatc cggaggacct ggtcatgtac caccacatcc ttgccgaggg ttgggattgc
     1741 gcctttggct cgaggttcat gcccggtgcg gtggtgaccg attacccccg gctcaagctc
     1801 ctggtcaacc ggatggcaaa tgccttcgtc aacgtgctct tccggctggg gtacaacgac
     1861 acgaccaacg ccttcaaggc ctaccggcgc gaggtcatcg agacgatcgg cccgctgctt
     1921 tccaagcact tcaacctgac cgtggagatg ccgctcaagg cggtcactcg cgggcacagc
     1981 ttcaaggtcg tgcccacctc ctggaccaac cgcaccgccg gtgaagcgaa actggcgatg
     2041 cgcgagatgg gcagccgcta cctcttcatc gtgctctaca tctggctcga ataccacctg
     2101 agccgaggcg attaccgcaa ggccgaaggg tctggttgac ctccggcgga tcgactttcc
     2161 gggactggct gggccggacc gaggaggtaa atccccgcag ttggctggtc ggtctggtgg
     2221 tcgcgctcct gggctggcag gtcagtctcc aggcaccggt atacggactt gacccgagct
     2281 ggtggtccgg tctctacatg gcctcccacg agaagatgcg gttcggcacc gagatcgtct
     2341 tcacctacgg tccgctggga atcctgcggg agccctggct cttttatccg ggcctgctca
     2401 gcctgatccc cttcctctac aaggcggccc tcttcaccgg attctgcatt gccctcgtcg
     2461 ccgtgctgag gaggcggatc ggctggcttt tcgccgcgct cgccgcgctc ctgctgctgc
     2521 tcctgctccg gcagatcgaa ctggcggtgg cgatttcgat gctggtttcg atcctgatca
     2581 tcgagcggtc ccccgacgac cgggtgatgc ccttcctggc gatcggcggc ggcctgctgg
     2641 ccggcattga gatgctgggc aaactctcgg ccggaccggt ggtcgccgtc gtgctgctgc
     2701 tcggtctgat cggggctcac gcgaggatgc g
//
LOCUS       DAOQFI010000209         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19495_length_2725_cov_2.882022, whole genome shotgun sequence.
ACCESSION   DAOQFI010000209 DAOQFI010000000
VERSION     DAOQFI010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19495_length_2725_cov_2.882022"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLE93_04605"
     CDS             complement(<1..897)
                     /locus_tag="PLE93_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNH86363.1"
                     /translation="MADSRNLLRARQFFSPERSAEGWSETSLRPRDWEQGYRDRWQHD
                     RIVRSTHGVNCTGSCSWKIHVKDGLVTWETQQVDYPSNGAETPDYEPRGCPRGASFSW
                     YVYSPLRPKFPYVRGELLSMYREARQRLGDPVEAWASIVDDPEKARAYKSRRGKGGFL
                     RASWNEVTEIAAAAHVHTIKKYGPDRIAGFSPIPAMSQVSYAAGSRFLSLIGGVPLSF
                     YDWYADLPPASPQVWGDQTDVPESADWWDAAYLIMWGSNIPQTRTPDAHFMTEARYRG
                     QKTIVVSPDYAGNTKFADQWLPA"
     gene            complement(1113..1751)
                     /locus_tag="PLE93_04610"
     CDS             complement(1113..1751)
                     /locus_tag="PLE93_04610"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNH86364.1"
                     /translation="MQVAYLLYDRFTALDITGPHEVFNSVPDVESIFVGEKTGPYRNE
                     SDTLSIVADRSLDEVMAPDILVIPGGFGTRALLDHEPLLDWIRTVDETTTWTTSVCTG
                     ALLLAAAGLLDGRPATTHWLARETLAGLGANPVPDRVVFDGKYVTGAGVSAGIDMALT
                     LVTEIYGPEIAQAVQLGIEYDPQPPHDAGAPEKAAPEIVDVVTAVFTAAETG"
     gene            complement(1771..>2725)
                     /locus_tag="PLE93_04615"
     CDS             complement(1771..>2725)
                     /locus_tag="PLE93_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HNH86365.1"
                     /translation="PLDVVGPAEVFAGASDLTAARVEGEATNPAYEVEVVAEEAAPLS
                     GRTSGYAILPAKTIDRCRGSIDTLIVAGGTGVLEAERSEPLVRWIGKAAGRSRRVASV
                     CSGSLLLAATGLLDGHRATSHWASCSELSRRYPEVEVDPDPIFVQDGRIWTSAGVTAG
                     MDLSLALVAEDLGEEVALEVARWLVLFLRRPGGQAQFSSHVSAPASRPRLRELQLWMA
                     DHLDSDLRVETLADRAMMSPRNFARAFRRETGMTPGTYVETLRLERARQLLEGPPGSI
                     DGIAVQCGFGTPETMRRSFARRLGVSPAEYRRRFRREPAEV"
BASE COUNT          434 a          875 c          961 g          455 t
ORIGIN      
        1 ggcgggcagc cactggtcgg cgaacttggt gttgccggcg tagtcgggcg agacgacgat
       61 cgtcttctgg ccgcggtagc gggcctcggt catgaagtgg gcgtcgggcg tgcgggtctg
      121 cgggatgttc gaaccccaca tgatcaggta ggcggcatcc caccagtccg ctgactcggg
      181 cacgtcggtc tggtctcccc agacctgcgg cgaggccggg ggcaggtcgg cgtaccagtc
      241 gtagaagctg agcggcacac cgccgatcag ggagaggaag cggctgccgg cggcgtagga
      301 gacctgggac atggccggga tcgggctgaa gccggcaatg cggtcgggtc cgtacttctt
      361 gatcgtgtgg acgtgggccg cggcggcgat ctcggtgacc tcgttccagc tggcgcgcag
      421 gaagccgccc ttgccccggc gtgacttgta ggcccgggcc ttttccgggt catcgacgat
      481 cgaggcccag gcctcgaccg ggtcgcccag gcgctgtcgc gcctcgcggt acatcgaaag
      541 gagttcgcca cgcacgtacg ggaacttggg ccgaagcggc gagtagacgt accaggaaaa
      601 ggaggcgccg cgcgggcatc cccgtggctc gtagtcgggc gtctcggcac cgttggaggg
      661 gtagtcgacc tgctgggtct cccaggtgac cagcccgtcc ttgacgtgga tcttccagga
      721 acaggaaccg gtgcagttca ccccgtgggt cgaacgaacg atccggtcgt gctgccagcg
      781 atcgcggtag ccctgttccc agtcccgcgg cctgagcgaa gtttcgctcc agccttcagc
      841 ggatcgttcc gggctgaaga actgccgggc gcgaagcagg ttgcgtgagt cagccacgag
      901 tggcgtctcc ggcgcaagat tggtgggaac tccgaggtgg gatcatttcc ccttccggtg
      961 acggttcaga cttgttgcgc caacatattg gattgaacgg tcgctattcc agtacttttc
     1021 cgcagacttc ctgcgggttg ctcacactca gttgttgccc tggcagcggg gctcccaaac
     1081 cggcggcagc agggtcaccc gtcgcgtgtc actcagcccg tttcggccgc cgtgaaaacg
     1141 gccgtgacca cgtccacgat ctccggtgcg gccttctcgg gtgcaccggc atcgtgcggg
     1201 ggctgcgggt cgtattcgat cccgagctgg accgcctggg cgatctccgg gccgtagatc
     1261 tcggtgacca gggtcagagc catgtcgatg ccggccgaaa cgcccgcgcc ggtcacatac
     1321 ttcccgtcga agacgacccg gtcgggaacc ggattcgcac cgaggccggc cagggtttcc
     1381 cgggcaagcc agtgagtggt cgccgggcgg ccgtcgagca ggccggcggc ggccaggagc
     1441 aaagcgccgg tgcagacgga ggtcgtccag gtcgtcgtct cgtccaccgt gcggatccag
     1501 tcgaggaggg gctcgtgatc cagcagcgcc cgggttccga agccgccggg gattaccagg
     1561 atgtccggcg ccatcacctc gtcgagggac ctgtccgcga cgatcgagag cgtgtccgac
     1621 tcgttgcggt acggcccggt cttctcaccg acgaagatcg attcgacatc cggcaccgag
     1681 ttgaagacct cgtgtgggcc ggtgatgtcg agggcggtga aacggtcgta gagcaggtat
     1741 gcgacttgca tggttctcct tgggtcgggt tcagacttcc gccggttcgc ggcggaatcg
     1801 gcggcggtat tcggcgggtg aaacgcccag ccggcgggcg aaagaccggc gcatggtttc
     1861 gggggttccg aaaccgcact ggacggctat cccgtcgatc gagccgggtg gtccttcgag
     1921 cagctgtcgg gccctttcga gtctcagcgt ctcgacgtat gtccccggcg tcatcccggt
     1981 ttcccgacgg aacgcgcggg caaagttgcg ggggctcatc atcgcccggt cggccagggt
     2041 ctcgactcga agatcggaat cgagatggtc ggccatccag agctggagct cgcgaaggcg
     2101 gggccgggaa gcgggagccg agacatgcga actgaactgg gcctggcctc cgggtcgacg
     2161 gagaaagagc accagccagc gggccacctc gagcgccact tcctcgccga ggtcctcggc
     2221 gaccagggcg agagataggt ccatcccggc cgtgaccccg gccgaggtcc agatgcgtcc
     2281 gtcctggacg aagatcggat cggggtccac ctccacttcc gggtagcgac gggaaagctc
     2341 ggaacaggaa gcccagtgac tggtcgcccg atggccgtcg agcagaccgg tggccgccaa
     2401 aagcagtgaa ccgctgcaga ccgaagcgac ccggcgggac cggccggccg ccttcccgat
     2461 ccaccgcacc agcggctcgg acctttcggc ctccagcaca ccggtcccgc cggcgacgat
     2521 cagcgtgtcg atcgaccccc ggcagcggtc gatcgtcttc gcgggaagga tcgcgtagcc
     2581 gctggtccga ccgctcaggg gcgccgcctc ttccgccacc acttccacct cgtaggccgg
     2641 gttcgtcgct tcaccttcga ccctggctgc ggtcaggtcg ctggcgccgg cgaagacctc
     2701 ggccgggccg accacgtcaa gtggc
//
LOCUS       DAOQFI010000210         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19759_length_2704_cov_3.351076, whole genome shotgun sequence.
ACCESSION   DAOQFI010000210 DAOQFI010000000
VERSION     DAOQFI010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19759_length_2704_cov_3.351076"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLE93_04620"
     CDS             <1..321
                     /locus_tag="PLE93_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86366.1"
                     /translation="GNLDFSSSGGMKVFSSANCTGSVNATIVPWSGLTPVQQAQFGAN
                     LRYGFSAAQQEDWYTRAITYLMAGQNQTNNVPASACNQQAPFTPIGDESQPPTQYLHV
                     FREP"
     gene            564..1670
                     /locus_tag="PLE93_04625"
     CDS             564..1670
                     /locus_tag="PLE93_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86367.1"
                     /translation="MKKLVAIVVALIASLSMAASAQAANETVEGTTNISAINGPLYQT
                     KAVPVNLMIRAEVTTPAESPKVNPLKNSKITFPAGVSFNPNNSKTPACTDNMLSVTSN
                     LADPSGVVASCEDSVVGTGTSAIVLAKVNLPETLIEDPILVLFNAGTDSKGQAKVKIY
                     GYSKTTNVGILMHGTLVKGVLDVAVPVLSDDSAVKYYQFDLPGPGLDRDDINVHTEGK
                     DPNYVHGLCPASGVLKTNSEFILGERSYPSGTDTTPETTVDSPETTQNCTGQVGAPKL
                     SASVTGPKSVKSGAKGTFKVKVTNKGTGVAKSVSVTGTGGAKGSTKNINPDTAKTVTV
                     KAKVTGRKGSKKTLTFTAKSGKSSAKATIKVTVK"
     gene            1741..>2704
                     /locus_tag="PLE93_04630"
     CDS             1741..>2704
                     /locus_tag="PLE93_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86368.1"
                     /translation="MGLKMRKLIYPLVLAAAWLGMAAAADAANESIGLSMEATPGPGG
                     LYREVARPIGASLTVTVTVPSESPKVTPIKVANVTFPEDMDFFPNKEKTPPCPDSALN
                     SQSNLAAGIAAIVDLCPKSVIGTGSSVVKLAKNNKDSFANLTDPRLVIFNAGRNGKGR
                     PKIVIYGYSKSVNAGLLMHGTLASDGQLRIEIGVMPFDSAVSEFTLGIPGEDLEVPDD
                     SAPGGSRTVKGLDRAYLRGKCSTGTWLATGKFVLGERSDPSGVPVGEETLLSSNPFEL
                     PCEGKAGHAKLAIRAIKGPRKIKARGKAGYVLTISNPGTATATNL"
BASE COUNT          576 a          876 c          785 g          467 t
ORIGIN      
        1 gggaaccttg acttttcctc cagcggtggc atgaaggtct tctcttccgc caactgcacc
       61 ggctcggtca acgcgaccat cgtgccctgg agcggcctta ccccggtcca gcaggcccag
      121 ttcggggcca acctgaggta cggcttcagt gccgcccagc aagaggactg gtacacgcgg
      181 gccatcacct atctgatggc cggccagaac cagaccaaca acgtgccggc atccgcctgc
      241 aaccagcagg ctccgttcac gccgatcggc gacgagtccc agccgccgac ccagtacctg
      301 cacgttttcc gcgagccgta aatccgggca gggtttttac ttcaggccag gcaattggcc
      361 ggattccgct cttggtccgg atttggctcc ctaagcgaca aattggcgat ccgctgcatc
      421 atcattgcga ccgatctcca aaagcaccgc aactcaggcg tcatattcgg gttctcgttc
      481 atgtaagctg cgaccggtta gggagtggga gcggctgact tgtcgcaacc acgtgtaatc
      541 cacaggagag aagagagacc cagatgaaga agcttgtagc catagtcgtg gcgcttattg
      601 cgtcactttc gatggcggcc tccgcccagg cggcgaacga gaccgtcgaa ggcaccacca
      661 acatcagcgc gatcaatggt ccgctgtacc agaccaaggc agtgccggtg aacctgatga
      721 tccgtgccga agtcaccact ccggccgaat cacccaaggt gaacccgctg aagaactcga
      781 agatcacctt ccccgcgggc gtgagcttca acccgaacaa cagcaagacc ccggcctgca
      841 ccgacaacat gctcagcgtc acgtcgaacc tggccgatcc gtcgggtgtc gtcgcgtcct
      901 gcgaggactc cgttgtcggc accggtacct cggcgattgt ccttgccaag gtcaacctgc
      961 cggagaccct gatcgaggac ccgatcctgg tcctcttcaa cgccggcact gacagcaagg
     1021 gccaggccaa ggtcaagatc tacggttact cgaagaccac caacgtcggc atcctgatgc
     1081 acggcacgct ggtcaagggt gttctcgacg ttgccgtccc cgttctctcg gacgactcgg
     1141 ccgtcaagta ctaccagttc gacctcccgg gcccgggcct ggaccgcgat gacatcaatg
     1201 tccacaccga gggcaaggac ccgaactacg tccacggcct ctgcccggcg tcgggtgtcc
     1261 tgaagacgaa ctccgagttc atcctcggcg agcgctcgta cccgagcggc accgacacca
     1321 ctcccgagac caccgtcgac tcacctgaga ccacgcagaa ctgcaccggt caggtcggcg
     1381 ctccgaagct ctccgcttcg gtcactggtc cgaagtcggt caagagtgga gccaagggca
     1441 cgttcaaggt caaggtcacc aacaagggca ctggtgttgc caagtcggtc agcgtgaccg
     1501 gcaccggcgg cgccaagggc tcgaccaaga acatcaaccc tgacaccgcc aagaccgtca
     1561 cggtcaaggc gaaggtcacc ggtcgcaagg gcagcaagaa gacgctgacc ttcacggcca
     1621 agagcggtaa gtcctccgcc aaggccacca tcaaggtgac cgtcaagtag gactctccta
     1681 ctggcagaag ttcgcaagaa gggcggccct ccggggccgc ccttttgctt tcctctcgcg
     1741 atgggtttaa agatgagaaa gctgatctat cccctggttc tggcagcggc ctggctgggg
     1801 atggcggccg cggctgacgc tgccaacgag agcatcggac tctcgatgga ggccactccc
     1861 ggtccgggag ggctgtaccg tgaagtcgcc cgaccgatcg gggccagtct cacggtcact
     1921 gtcacggtgc cgtcagagag cccgaaggtg acacccatca aggtggccaa cgtgaccttc
     1981 cccgaggaca tggacttctt cccgaataag gagaagaccc cgccctgtcc cgacagcgcc
     2041 ctgaactctc agagcaacct ggccgctggc atcgctgcga ttgtcgatct ctgtccgaaa
     2101 tcggtcattg gcaccggttc ctctgtggtc aagctggcca agaacaacaa agattcgttc
     2161 gccaacctca ctgacccccg tctggtcatc ttcaacgccg gtcggaacgg caaaggccga
     2221 ccgaagatcg tcatctacgg ctattcaaag tcggtcaacg ccggactgct gatgcacggc
     2281 accctggcca gcgatggcca gctcagaatc gagatcggcg taatgccgtt tgactctgcg
     2341 gtcagcgaat tcacgcttgg tattcccggc gaagacctgg aggttcctga tgattctgcc
     2401 cccggaggaa gcaggaccgt caaagggctt gatcgggctt acctgagggg caagtgttca
     2461 accggcacct ggctggcaac cggaaagttt gtgctgggag agaggtccga tccgagcggc
     2521 gtgccggtcg gcgaagaaac gctgctctct tccaacccct tcgagcttcc ctgtgaggga
     2581 aaggccgggc acgcgaagct ggcgataagg gcgatcaaag gaccgaggaa gatcaaagcg
     2641 cgcggcaaag cgggatatgt cctcacgatt agcaacccgg gcacggccac cgcaaccaac
     2701 ctgc
//
LOCUS       DAOQFI010000211         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19872_length_2695_cov_3.317424, whole genome shotgun sequence.
ACCESSION   DAOQFI010000211 DAOQFI010000000
VERSION     DAOQFI010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19872_length_2695_cov_3.317424"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1316
                     /locus_tag="PLE93_04635"
     CDS             102..1316
                     /locus_tag="PLE93_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86369.1"
                     /translation="MTATTTEADDMDRRSIAVLAAGHAMVDACQGAIPATLPFLIARR
                     GYSYAEATSLLLVMTVCSSLLQPLFGYITDRRSISWLLPGGVLLGGLGVAAVGLVHSH
                     ALTLAAVGIAGLGVGAYHPEGARNANFVAGNRRRATAMSLYGVGGNAGFAVGPVIVTG
                     LVLAFGLGGLVWFALPMTLGAIWLARELPRIDSLRLATIAGAEAQPGGRGEDRWLPFA
                     GIATAAGFRSAAYFGLQAFVPIFLITTMGTSDAVGNAALTVLLASGVAGTLLGGALAD
                     RVGNKPVLVTSLGVASPLILGLLAVNEAGAFALVALIGFFLFGSFSIAVVLGQQYLPN
                     RIGVASGVTLGAAIGFGGLVAWLLGLLADQTSLTTVMIVIAALPLPGFLISLALPGEN
                     EAGQPSEEPARV"
     gene            1313..2461
                     /locus_tag="PLE93_04640"
     CDS             1313..2461
                     /locus_tag="PLE93_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH86370.1"
                     /translation="MSGEEYNFTDLSLDRLRRRRSAKWTFWPDDVLPAFVAEMDFPLA
                     RPVKEALVQAIELDDTGYSNASASGLADAFAAFADRRFGWEVDPEQVTATTDVVGGLR
                     ALLDLATGPLEQVIINPPVYFPFFSIIPEVGSEILEVPLDEKGRLDLDGTAAAFAGGA
                     RAMILCSPHNPTGSVPARTELERLAEIAAESDAWILADEIHAPLTLPGATHTPFLDVS
                     PAARECGICVTSASKAFNIAGLSCAVIVTASERARRLVSRLPAGATHPGHLGVIASQA
                     AFESGDQWLDQVRDQLDFNRRLLSDLLAGHLPAARYRPPEAGYLAWVDAGELDLGPDP
                     ASEILDRARVAVSSGPGFGSGGEGYFRLNIGTSPQLVEAAVRSISEIA"
     gene            2458..>2695
                     /locus_tag="PLE93_04645"
     CDS             2458..>2695
                     /locus_tag="PLE93_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86371.1"
                     /translation="MTPERRQPKDKTQPTQEVLLNDLRRSRSVGEAAEVTARAAVEQF
                     GAVGAVVSDRQGPIAARPTRLDPLTIAELTAIPEG"
BASE COUNT          386 a          927 c          910 g          472 t
ORIGIN      
        1 cgggccattt ccctggcctt tcgcaccggc gagccgtaat ccggatagtt ggtcacgggg
       61 ttcgggtcgg tcccgggcgg aacttctgac aggcttgacc ggtgaccgca acgaccacag
      121 aggccgatga catggaccgg cgctcgatcg ccgtgctcgc ggccggccac gccatggtcg
      181 atgcctgcca gggagcgatc cctgcaacgc tgcccttcct gatcgcccgt cgcggttaca
      241 gctacgcgga ggcgacttcg cttctgctgg tcatgaccgt ctgctcgtcc ctgcttcagc
      301 ccctcttcgg ctacatcacg gaccgccggt cgatctcatg gcttctgccc ggcggggtgc
      361 tgctcggcgg gctcggtgtg gccgcggtcg ggctggtcca ctcgcatgcc ctgacccttg
      421 ccgcggtggg aatcgccggt ctgggagtcg gcgcctacca ccccgaaggg gcccgcaacg
      481 cgaactttgt ggccggaaac aggcgaaggg cgacggcgat gagcctttac ggggtcggag
      541 gcaacgcggg tttcgcggtc ggtccggtga tcgtgaccgg gctcgtcctg gcattcggtc
      601 tcgggggact ggtctggttc gccttgccga tgaccctggg tgcgatctgg ctggcccgcg
      661 agctgccccg catcgattcc ctgcgcctgg caacgatcgc gggagccgaa gcgcaacccg
      721 gaggtcgcgg tgaagaccgc tggctgccgt ttgcggggat cgcgacggcg gccgggttcc
      781 gctccgccgc gtatttcgga ctccaggcct tcgtgccgat ctttctgatc acgaccatgg
      841 gcacctcgga cgcggtcgga aacgccgccc tgacggtgct gctggcgagc ggggttgcgg
      901 gaaccctgct gggcggggca ctggccgacc gggtcggcaa caagccggtc ctggtgacct
      961 cgctgggcgt ggccagcccc ctgatcctgg ggctgctggc ggtaaacgaa gccggtgctt
     1021 ttgccctggt ggccctgatc gggttcttcc tcttcggcag cttctcgatc gcggtcgttc
     1081 tcggccagca gtacctgccc aaccggatcg gtgtggcctc cggggtcacg ctcggcgccg
     1141 ccatcggctt cggcgggctg gtcgcctggc tgctcggact gctggccgat cagaccagcc
     1201 tgaccacggt gatgatcgtg attgccgcct tgccgctacc gggattcctg atcagcctgg
     1261 cgctgcccgg agaaaatgag gccggccaac caagcgagga gccagcccgg gtttgagcgg
     1321 cgaggagtac aacttcacag acctttctct cgaccggctg cggcgccgtc gctcggccaa
     1381 gtggaccttc tggcctgacg acgtcttgcc agccttcgtt gcggagatgg atttccccct
     1441 ggcccggccg gtcaaggagg ccctggtcca ggcgatcgaa ctcgatgaca ccggctattc
     1501 caacgcctcc gcgtccgggc tcgccgacgc gttcgcggct ttcgcggacc gtcgcttcgg
     1561 ctgggaggtg gatccggaac aggtcaccgc caccaccgac gtggtcggcg ggctgcgggc
     1621 gctgctcgac ctcgcaaccg gtccgctgga gcaggtgatc atcaatccgc cggtctattt
     1681 cccgttcttc tcgatcattc ccgaggtcgg cagcgaaatc ctggaagtgc cgcttgacga
     1741 aaagggccgg ctcgatctcg acgggaccgc cgccgccttc gccggcggcg ccagggccat
     1801 gattctctgc agtccgcaca acccgaccgg ctccgttccg gcccggaccg agctggaacg
     1861 actggcggaa atcgccgccg aaagcgacgc ctggatcctg gccgacgaga tccacgcgcc
     1921 gctcaccctg ccgggagcga cccacacacc gttcctcgat gtctccccgg cagcgcgcga
     1981 gtgcgggatc tgcgttacct cggcctcgaa ggcgttcaac atcgccggtt tgagctgcgc
     2041 ggtgatcgtg acggccagcg agcgggcgag gcggctcgtt tcccggctgc cggccggcgc
     2101 cacccatccg ggtcacctcg gggtgatcgc ctcgcaggcg gcattcgaat ccggagatca
     2161 gtggcttgac caggtcaggg accagctcga cttcaaccgg cggctgcttt ccgacctgct
     2221 cgccgggcat ctacccgccg cccgataccg gccgcccgag gccggatacc tggcctgggt
     2281 cgatgccggc gagctcgatc tcggccccga tcccgcctcg gagatcctcg accgggcccg
     2341 ggtcgcggtt tcgagcggac ccggattcgg cagtggtggc gaagggtatt ttcgcctcaa
     2401 cattggcact tcaccacaac tggtcgaggc tgccgtaagg tcaatctcag agatcgcatg
     2461 actccggaac ggcgccagcc caaagacaag acgcagccca cgcaggaggt actcctcaat
     2521 gacctgcgcc ggtcacggtc ggtcggcgag gccgccgagg tgacggcccg ggcggcggtc
     2581 gaacagttcg gagcggtcgg cgcggtggtc agtgaccgcc agggcccgat cgccgcccgg
     2641 ccgaccaggc tcgacccgct gaccatcgcc gaacttacgg cgattcccga aggag
//
LOCUS       DAOQFI010000212         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19909_length_2692_cov_3.403110, whole genome shotgun sequence.
ACCESSION   DAOQFI010000212 DAOQFI010000000
VERSION     DAOQFI010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19909_length_2692_cov_3.403110"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLE93_04650"
     CDS             complement(<1..1125)
                     /locus_tag="PLE93_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNH86372.1"
                     /translation="MNGNLARELANLLSPEQVVTIGPELRAALQDGTGNRGIKGEAEA
                     LLLPADAEEVAEVMAWCYGHGVPLTVRGGGTGLAGGAVPDGGVVLSLERLARVRSIDP
                     ELWRMEVEAGVRTATVHRIALENGLMFPPDPGASEQSTIGGNIATNAGGPHAFRYGVT
                     GAWVTGIEAVVPPGEVLSSGGPLRKNVAGLDLTDLMIGSEGTLGIVTSAWLRLVPAPE
                     ERGVVVGFYPDTASGCEAIGMIMGSGIQPTALEFLDRGALESSLGSFPSEAPEQAGFA
                     VIAEADGSAGEVNQAVGDLEEAMAGGALQPPVRITDHVAIRNFWAWRDGVSIAVTAQR
                     GGKISEDVVVPVESLAAAVDETVEIGRRHDLPACSWGHAGD"
     gene            1262..>2692
                     /locus_tag="PLE93_04655"
     CDS             1262..>2692
                     /locus_tag="PLE93_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNH86373.1"
                     /translation="MGLTGGEFVAEYLIAEGVPYVFGIPGHGDMAAFDAFKDRADRIT
                     MIGPRHEQAAAHMADAYYRASGKPLATMTSIGPGSMNVATGLGTAFVDSIPLISFTGG
                     PQTYMLGRGVLQELERQQDNVFPHIIQPMVKRNWDVQHVDLLADVLPRAFNNMLSGRP
                     GPVHIELPMDVQAEVTELPVPDPAERRATSGAVRPDPDAIDKAAAKLLAAKRPVLLAG
                     GGCLEAKAAAEVKKVAEYLDAAVVVTMMGKGVFPEDHPLDAEHTGSNGTLVGNYMTKN
                     ADVILAVGTRFAEQTSSSFMDGMSFSIPPTEIIHLDIDPTEIGKNYPTAVGAVCDAKA
                     GLNDLFDALVDHNGTEGISRPAYREEIAKLRADWKEMLTGRWTGKLSLSQVLHKSREV
                     LPREAIAIASAGHPQIQAFQEFLSYEPRTWLSPGGYSTMGYTLPAAIGAKLAKPDVPV
                     VGFAGDGDLQQTIQELAVAAETGAAVI"
BASE COUNT          470 a          918 c          869 g          435 t
ORIGIN      
        1 gtccccggcg tgaccccagg aacaggccgg caggtcgtga cggcggccga tctcgaccgt
       61 ctcgtcgacc gccgcagcca gcgactcgac cggaaccacg acatcctcgg aaatcttgcc
      121 gccgcgctgg gcggtgacgg cgatcgaaac tccgtcccgc caggcccaga agttccggat
      181 tgcgacatgg tcggtgatcc tgaccggcgg ctgaagtgcc cctccggcca tcgcctcttc
      241 caggtccccg accgcctggt tcacctcgcc ggcggaaccg tcggcctcgg cgatcacggc
      301 aaaacccgcc tgctccggtg cctcggaagg aaaggaaccg aggctggatt cgagcgcgcc
      361 gcggtcgagg aactcgagcg ccgtcggctg gatcccggaa cccatgatca ttccaatcgc
      421 ctcgcagccg gaggcggtgt ccgggtagaa gccgaccacg acgccgcgtt cctcgggggc
      481 cggcaccagc cgcagccagg ctgaggtgac gatcccgagg gttccttccg aaccgatcat
      541 caggtcggtc aggtcgaggc cggcgacgtt cttcctcagc ggcccgccgg aggaaaggac
      601 ctctcccggt ggcacgaccg cctcgatgcc ggtcacccag gccccggtca ccccgtaccg
      661 gaatgcgtga gggccgccgg cgttggtggc gatgttgccg ccgatcgtgg actgttcgct
      721 ggcccccggg tcgggaggga acatcagtcc gttctcgagc gcgatccggt gcactgtggc
      781 cgtccggacc ccggcctcga cttccatccg ccagagctcg ggatcgatcg aacgaacccg
      841 ggctagccgt tccaggctga gtacaacccc gccatccggc acggccccac cggccagtcc
      901 ggtgccaccc ccgcgcacgg tcagcggcac gccatgcccg tagcaccagg ccatgacctc
      961 ggccacttct tcagcgtcgg cgggcaggag gagagcctcg gcctcgcctt tgatgccgcg
     1021 gttgccggtt ccgtcctgca gggcagctct caattcgggg ccgatagtga ccacctgttc
     1081 aggcgaaagc aggttcgcca gttcccgggc aaggttgccg ttcacgattc gcttttcaga
     1141 aattcctgat tccaatgcgg gttcgtccct cagctccccg gatcctgatt gcaaggtagc
     1201 cggtagtagg taatatgtta cctgctatag cgtgggcatc agcccggagg aaggagaaca
     1261 ggtgggactg accggaggag aattcgtcgc cgagtatctg atcgcggagg gggttccgta
     1321 tgtattcgga attcccggtc atggcgacat ggcggcattc gatgcattca aggaccgggc
     1381 cgaccggatc accatgatcg gcccgcggca cgagcaggcg gctgcccata tggccgatgc
     1441 ctattaccgg gcgtcgggca aacctctggc cacgatgacc tcgatcggtc cgggttcgat
     1501 gaacgtggcc accggtctcg ggaccgcctt cgtcgattcg atcccgctga tctcattcac
     1561 cggaggcccc cagacctaca tgctcggccg cggtgtcctg caggaactcg aacgccagca
     1621 ggacaatgtc ttccctcaca tcatccagcc gatggtcaag cggaactggg atgtccagca
     1681 tgtggacctg ctggctgatg ttctgccgcg ggccttcaac aacatgctct cgggccggcc
     1741 cggtccggtt cacatcgagc tgccgatgga cgttcaggcc gaagtgaccg aactgccggt
     1801 tccggacccg gccgagcggc gggccacatc cggcgcggtg aggccggacc cggacgcgat
     1861 tgacaaggcg gcggcgaagc tgctcgcggc gaagcggccg gtgctgctgg ccggcggcgg
     1921 ctgcctcgag gcgaaggcgg cggccgaggt gaagaaggtc gccgaatatc tggacgcggc
     1981 cgtggtcgtg accatgatgg gcaagggggt cttccccgag gaccatccgc tcgacgccga
     2041 acacaccggc tccaacggca ccctggtcgg caactacatg accaagaacg cggacgtgat
     2101 cctcgcggtc ggcacccgct ttgccgagca gacctcctcc tccttcatgg acgggatgtc
     2161 cttctcgatt ccgccgaccg agatcatcca cctcgacatc gacccgaccg agatcggcaa
     2221 gaactacccg accgcggtcg gcgcggtctg tgacgcaaag gccggtctca acgatctctt
     2281 cgacgcgctg gtcgatcaca acggaaccga aggaatcagc cggcccgcct accgggaaga
     2341 gatcgcgaag ctgcgggccg actggaagga aatgctgacc ggccgctgga ccggcaagct
     2401 ctcgctcagc caggtcctgc acaagtcacg ggaggtattg ccgcgggaag cgatcgcgat
     2461 cgcctcggcc ggccaccccc agatccaggc cttccaggag ttcctctcct atgaaccgcg
     2521 gacctggctc agcccgggcg gttactcgac catgggctac accctgccgg ccgcgatcgg
     2581 agccaagctg gccaaaccgg atgtgccggt ggtcggcttc gccggtgacg gtgacctcca
     2641 gcagacgatc caggagctgg cagttgccgc cgagaccggc gcggccgtga tc
//
LOCUS       DAOQFI010000213         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19932_length_2690_cov_2.662619, whole genome shotgun sequence.
ACCESSION   DAOQFI010000213 DAOQFI010000000
VERSION     DAOQFI010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19932_length_2690_cov_2.662619"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1637)
                     /locus_tag="PLE93_04660"
     CDS             complement(159..1637)
                     /locus_tag="PLE93_04660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86374.1"
                     /translation="MSPFSQSSAGSDRPHPTRTLLILSLAALSFALAQTTLIPALAEL
                     MEEFHTDATGIAWVLTGYLISAAIFTPIFGRLGDMFGKRRLLVIALSIFAAGSTVSAL
                     GDSVPAIVAGRILQGVGGGIFPLCFGIIRDEFPPEKVSASVGLMSATLGIGGGIGLVV
                     GGLIVDHASYHWIFWLGAAMAVMAAAAALIFVPESPNRVPAKVDYRGAAVLAVGLSVP
                     MYAISEANNWGWTSTKTIGLILAGFVVITFWVWLQKRTREPLADVSLLAEPRVAMTNT
                     ATLLIGFGMFASFILIPQLVESPDSTGYGFGFSATQAGLVMLPAALMMLPAGPLSGHL
                     GARFGNRVPLALGGLISGIGLIGLGLDHGSALSIVLWNVLSSIGVGLAYAAMPNLIME
                     AVPLERTGEATGFNTLIRSVGSSLGSQVTASILTGTILAATGFPTSDGFRTAFLVGAG
                     VSIFAGLLALLIPRSGHRHAIFTEEIGAASVLPEPALAADER"
     gene            complement(1677..>2690)
                     /locus_tag="PLE93_04665"
     CDS             complement(1677..>2690)
                     /locus_tag="PLE93_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HNH86375.1"
                     /translation="GYFSQRGHRVMLGDIDRQTIAGAVSTGTHGTGRALPNISAQIEK
                     ARIMGADGEIRELEPGSDGLLAARVAVGSLGVLVSVTLRTVPAFNLHRVDAPMDLDQV
                     LAGFDRITEENEHFEFFVFPYTRTALTLIRNRTDQPLAPRSQADRFLGDVVVENGLGD
                     LALRLTGRFRSAIPKMARFSTGFMSQSEQIDVSHRVFANYRTIRFNEMEYALPAEHGP
                     EALDRVLELIETENFPLAMPIECRISAGDDALISQAHERPTVYIAVHQHRTMEYEPYF
                     REIERIFREYDGRPHWGKLHFRTAEDLAPSFPRWADFQKVRDELDPNRNFSNAYVTEV
                     LGA"
BASE COUNT          486 a          845 c          950 g          409 t
ORIGIN      
        1 gtggccgtgc tggccgccgc cccggccgca cacgccgtgc tcggcgaaga cctgttcggc
       61 cgcggcgatc actttctcgc gattgcggac cgcgtcggcg cggcgcggaa cctcgacgct
      121 ttcggttacc ccggcaggga gcccggcgtc gaccggattc atcgttcgtc ggccgccagg
      181 gcgggttcgg ggagaacgct ggccgcgccg atttcctcgg tgaagatcgc gtggcgatgg
      241 ccggagcgcg ggatcaggag ggcgagcaac ccggcgaaga tcgagactcc ggctccgacc
      301 agaaacgcgg tccggaaccc gtcgctggtc ggaaagccgg tcgcggcgag gatcgtgccg
      361 gtcaggatcg aggcggtgac ctgggagccc agcgaggagc cgaccgagcg gatcagcgtg
      421 ttgaagccgg tcgcctcacc ggtgcgttcc agcggcactg cctccatgat caggttcggc
      481 atcgccgcgt aggcgagtcc cactccgatc gaggagagga cgttccagag cacgattgaa
      541 agcgccgaac catggtcgag ccccagcccg atcagtccga tgccgctgat cagcccgcca
      601 agcgcgagtg ggaccctgtt tccgaaccgg gctccgagat gcccggaaag cggcccggcc
      661 ggcagcatca tcaaggctgc gggaagcatc accaacccgg cctgggtcgc cgaaaagccg
      721 aaaccgtagc cggtcgaatc gggtgactcg accagctgcg gaatcaggat gaatgaagcg
      781 aacatgccga agccgatcaa cagggtggcg gtgttggtca tcgcgaccct gggttcggcc
      841 agcagggaga catcggccag cggctccctg gtccgcttct gcagccagac ccagaaggtg
      901 atcaccacga atccggcgag gatcagtccg atcgtcttcg tgctggtcca gccccagttg
      961 ttggcctccg agatcgcata catcgggacc gaaagcccga ccgccagcac ggctgcgccc
     1021 cggtagtcaa ccttcgccgg aacccggttt ggtgactcgg gcacgaagat cagcgccgcg
     1081 gccgcggcca tcacggccat tgccgcgccg agccagaaga tccagtggta ggaggcgtgg
     1141 tcaactatca gcccgccgac gaccaggccg atgccgccac cgatgccgag cgtcgccgac
     1201 atcaggccga ccgaggccga gaccttttcg ggcgggaact cgtcacggat gattccgaaa
     1261 cagagaggga agattccgcc gccgacgccc tggaggatgc ggcccgcaac gatggcgggg
     1321 acgctgtctc cgagggccga gactgtggag ccggcggcga agattgagag ggcgatgacg
     1381 agcagccggc gcttgccgaa catgtctcca aggcgcccga agatcggggt gaagatggca
     1441 gccgagatca ggtagccggt gaggacccag gcgatcccgg tcgcgtcggt gtggaactcc
     1501 tccatcagtt cggccagggc cggaatcagg gtggtctggg ccagcgcgaa ggaaagcgcg
     1561 gccaggctga ggatcaggag cgtgcgggtc ggatggggtc ggtccgaccc ggcggacgat
     1621 tgactaaacg gactcaccgg tccggatagt aacagccaag cggacccgcg ggtccgctaa
     1681 gcgccgagta cttcggtcac ataggcgttg ctgaagttcc ggttcgggtc gagctcgtca
     1741 cgcaccttct ggaagtcggc ccagcggggg aagctcgggg cgaggtcctc cgccgtccgg
     1801 aagtggagct tgccccagtg cggccggccg tcgtactcgc ggaagatccg ttcgatctcc
     1861 cggaagtagg gctcgtattc catggtccgg tgctgatgga ccgcgatgta gacggtcggc
     1921 cgttcgtggg cctggctgat cagggcgtcg tcaccggccg agatccggca ctcgatcggc
     1981 attgccagcg ggaagttttc ggtttcgatc agctccagta cccggtcgag agcctccggc
     2041 ccatgctcgg ccgggagcgc gtattccatc tcgttgaagc ggatcgtccg gtagttcgca
     2101 aagacccggt gtgaaacatc gatctgctcc gactgggaca tgaagccggt cgagaaccgg
     2161 gccatcttcg ggatggccga ccggaaccgc ccggtcaggc ggagggcgag gtcaccgagt
     2221 ccgttctcga ccaccacgtc accgaggaag cgatcggcct gcgagcgggg ggcgagcggc
     2281 tggtcggtgc ggttgcggat cagggtcagg gcggtccggg tgtaggggaa gacgaagaac
     2341 tcgaagtgct cgttttcttc cgtgatccgg tcaaaaccgg cgagcacctg gtcgaggtcc
     2401 atcggcgcat cgacccgatg caggttgaag gcggggacgg tacgcagggt caccgagacc
     2461 agcacaccga gcgacccgac cgcgacccgg gcggccagca acccgtcgga gcccggttcc
     2521 agctcgcgga tctccccgtc ggctcccatg atgcgggcct tctcgatctg tgcggagatg
     2581 ttcggcaggg cacggccggt tccgtgagtc ccggtggaaa cggcgccggc gatcgtctgg
     2641 cgatcgatgt ccccgagcat cacccgatgg ccgcgctgcg agaaatagcc
//
LOCUS       DAOQFI010000214         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19966_length_2687_cov_2.294073, whole genome shotgun sequence.
ACCESSION   DAOQFI010000214 DAOQFI010000000
VERSION     DAOQFI010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19966_length_2687_cov_2.294073"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLE93_04670"
     CDS             complement(<1..817)
                     /locus_tag="PLE93_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNH86376.1"
                     /translation="MAEFKLNPAFKPLADQPAAIEQLTEGVEAGEKIQTLLGATGTGK
                     TFTMAGVIAETQRPALVMAHNKTLAAQLCNEFREFFPDNVVEYFVSYYDYYQPEAYVP
                     AQDLYIEKDSSINDEIDRLRHAATASLLARRDVIIVASVSCIYGIGSPALYQQQMQLF
                     KVGEWIDRDKVLRKLVRLQYERNDTVLSRGSFRVKGEVLEIMPSYAESAFRIMLFGDE
                     IEAIHHFDPLTGEVLDEAQLPLTYAGLSHCFRTEAGAAGRASKGLYRVHQFTKV"
     gene            851..1228
                     /locus_tag="PLE93_04675"
     CDS             851..1228
                     /locus_tag="PLE93_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86377.1"
                     /translation="MRTLRRALGLAFIGVGILHFARPKFFLSIMPSWIPLHREAVAVS
                     GAAEIVGGVAMLPDRTASLAAPWLVALLVAVFPANVNMAIDQQQIEQAKASGIPLWAL
                     WARLPLQPLLMYLVWKSTRPQDG"
     gene            complement(1233..2114)
                     /locus_tag="PLE93_04680"
     CDS             complement(1233..2114)
                     /locus_tag="PLE93_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86378.1"
                     /translation="MNSPGDEPTEVNPPGNEPTEVNPRGDQASQVIPPGSRPAAAPDP
                     GEQAGSRGPASRGQRGIGVLIGSIVASLVLVGAYALAGGPVAPDGPADPCDARPWTDP
                     QNLEEAAQQFALSAADGAACELGVSREELTRSIADDQARQDFMDEHGFSESDLEDALR
                     SGLNRAVDDAENAGAISGITPALLRAAIKVMPMDTMIGLLENADSIFNGDFGSMGGLG
                     DALNAITGGFDGLGNSGDPGNSNDPLGSLGDALEGALPEGTTDDLNQIIPEEERQKLE
                     DQAREKLKQGLEDFLGP"
     gene            complement(2168..>2687)
                     /locus_tag="PLE93_04685"
     CDS             complement(2168..>2687)
                     /locus_tag="PLE93_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86379.1"
                     /translation="TVWLVIPPQILAGLGLSLVLSALTEAALHGRSFAAVHGGWTISA
                     RHAGVVIGLLILTPIFTHQLDVQKEAALDAGTAIVLDSPIDPSAKIDLGDKLANEVEA
                     QGDRVPDLSPAFEPMPTDPEDHARYEEILTGIESQLKDAATKAFSLSFLISALFGLLA
                     LIPIGMTRRLDL"
BASE COUNT          426 a          869 c          907 g          485 t
ORIGIN      
        1 cgaccttggt gaactggtgg acgcggtaga gccccttgct cgcgcgcccg gcggcgccgg
       61 cctcggtccg gaagcagtgg gagagcccgg cgtaggtcag cggcagttgg gcctcgtcga
      121 ggacctcgcc ggtgagggga tcgaaatggt ggatcgcctc gatctcgtcg ccgaagagca
      181 tgatccggaa ggccgactcg gcgtaggagg gcatgatctc cagcacctcg cccttgaccc
      241 ggaaggagcc gcggctcagc accgtgtcgt ttcgttcgta ctgcagccgg accagcttgc
      301 gcaggacctt gtcgcggtcg atccactccc ccaccttgaa cagctgcatc tgctgctggt
      361 agagagccgg cgagccgatg ccgtagatgc atgagaccga agcgacgatg atcacgtccc
      421 gccgggccag cagcgaggcg gtcgcggcgt gacgcaggcg gtcgatctcg tcgtttatcg
      481 aggagtcctt ctcgatgtag agatcctggg ccgggacata ggcctccggc tggtagtagt
      541 cgtagtagga gacgaaatac tcgaccacgt tgtcgggaaa gaactcgcgg aactcgttgc
      601 agagctgggc ggcgagggtc ttgttgtgag ccattaccag ggccgggcgc tgggtctcgg
      661 cgatcacccc ggccatggtg aaggtcttgc cggtcccggt cgcgccgagc agagtctgga
      721 tcttctcgcc agcctcaacc ccttcggtca attgctcgat cgccgcaggc tggtcggcca
      781 gcggcttgaa ggctggattg agcttgaact cggccacccg cccaccataa cgacccgtta
      841 ggttcgaccc atgcgaactc ttcgccgcgc cctgggcctc gctttcatcg gagtcggaat
      901 ccttcacttt gcccggccga agttcttcct ttcgataatg ccttcgtgga tcccgctcca
      961 cagggaggcg gtcgcggtca gcggggccgc cgagatcgtc gggggtgtgg cgatgcttcc
     1021 cgaccggacc gccagcctgg cggcaccctg gctcgtcgcc ctgctggtgg cggtctttcc
     1081 ggccaatgtc aacatggcga tcgaccagca acagatcgaa caggcgaaag caagcggcat
     1141 ccccctctgg gcgctctggg cccgactgcc gctccagccg ctgctcatgt acctggtctg
     1201 gaagtcaacc cgcccgcagg acggatgacg gctcacggcc cgaggaagtc ctcgaggccc
     1261 tgcttgagct tctcccgggc ctgatcctcg agcttctggc gctcttcttc cgggatgatc
     1321 tggttgaggt cgtcagtggt gccctccggc agggcgcctt cgagggcgtc cccgaggctg
     1381 ccgagcggat cgtttgaatt gccggggtcg ccggagttgc cgaggccgtc gaaaccgccg
     1441 gtgatcgcgt tcagcgcatc accgagtccg cccatcgaac cgaaatcccc gttgaagatg
     1501 ctgtccgcgt tttcgagcag tccgatcatc gtgtccatcg gcatcacctt gatcgcggcc
     1561 cgcaggagcg ccggggtgat gccgctgatc gcgccggcgt tctcggcgtc atcgaccgcc
     1621 cggttgaggc cggaacggag cgcgtcttca aggtcggatt cgctgaaccc gtgctcgtcc
     1681 atgaagtcct gccgggcctg gtcgtcggcg atcgagcggg tgagttcttc ccggctgact
     1741 ccgagttcac aggctgcgcc gtcggcggcc gacagtgcga attgctgggc ggcttcctcg
     1801 agattctggg gatcggtcca ggggcgggca tcgcatgggt cggccgggcc gtccggggct
     1861 accggcccac cggccagcgc gtaggccccg accagaacca gcgaagcgac gatcgatccg
     1921 atcagcaccc cgattccccg ctggccccga ctggccggac cgcgcgaccc ggcctgttca
     1981 cccggatctg gtgccgccgc cggcctgctg ccgggcggaa tcacctgtga cgcttggtcg
     2041 ccgcgcgggt tcacctcggt cggctcgttc cccggcgggt tcacctcggt cggctcgtcg
     2101 cccggcgaat tcacctcggt cggatcctcc gggccgcccg gcatttcgtc tgtcggttcg
     2161 tccggggtca aaggtccagt ctccgggtca tgccgatcgg gatcagggcc agcagcccga
     2221 acagcgccga gatcagaaac gaaagcgaga aagccttggt cgcagcatcc ttcagctggg
     2281 attcgatccc ggtgagaatc tcctcgtacc gggcgtggtc ctcgggatcg gtcggcatcg
     2341 gttcgaaggc aggagagaga tccggcactc ggtcgccctg ggcttcaacc tcgttggcga
     2401 gcttgtcccc gagatcgatc ttggccgagg gatcgatcgg ggaatcgagc acgatcgcgg
     2461 taccggcgtc gagggccgcc tctttctgca cgtcaagctg atgggtgaag atcggagtga
     2521 ggatcaacag gccgatcact acgccggcgt gccgggcgga aatagtccag ccgccgtgga
     2581 ccgcggcaaa agagcgtccg tgaagggcgg cctcggtcaa cgccgacagg acgagggaga
     2641 gcccgagacc agccaggatc tggggcggga ttaccagcca gacggtc
//
LOCUS       DAOQFI010000215         2686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19974_length_2686_cov_3.170658, whole genome shotgun sequence.
ACCESSION   DAOQFI010000215 DAOQFI010000000
VERSION     DAOQFI010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19974_length_2686_cov_3.170658"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLE93_04690"
     CDS             complement(<1..714)
                     /locus_tag="PLE93_04690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNH86380.1"
                     /translation="MSKFGGREVVIVEAVRTPVGRGHKEKGYYKDTHPSTLLAHAYKN
                     VVERAGIDPGEVEDVVAGCVQQFGEQTLNIGRNAWLEAGFPIETPATTIDRQCGSAQQ
                     AINYAAAMIASGVHDVMIGGGVEHMGHISFAHSAKVMSEYGYAYSPELLEKYDLVNQG
                     ISAEMIADQWEIPKVDLDELAVRSHARAQQATEEGRFEREIAPFQVNGDTYMTDQGIR
                     PGTNLEALAGLKPAFKEDGK"
     gene            836..1663
                     /locus_tag="PLE93_04695"
     CDS             836..1663
                     /locus_tag="PLE93_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNH86381.1"
                     /translation="MTATPYEFVAIAGSGTIATGLAAVASTSSGKVVMLVRSEESADR
                     ALTAVEKASRRIPGANPSRVTTTMEIADLAEANLLVEAVVEDAGVKADLLRRISEVAP
                     RADLATTTSSLSITKLGEDSGAADRVYGLHVFNPVPAMQLVELIFPDPVTEEVAERAH
                     DWCHILEKTAVEVPDTAGFAVNRLLFPYLFDAVRYQERTGLEAAAVDQCMTLGVAHPM
                     GPLALLDLVGLDVAVAIGQALNNESGNPDHIAPATVQEFVAAGHLGRKTGQGFYEYG"
     gene            complement(1660..1938)
                     /locus_tag="PLE93_04700"
     CDS             complement(1660..1938)
                     /locus_tag="PLE93_04700"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNH86382.1"
                     /translation="MALLEDQEIQDRLAELDGWSLEGRRIVRTFEFDDFVGSINFVDS
                     LVQPAESMNHHPDLGISWNKVTVAITNHAEGGLTEKDFELASRIQALV"
     gene            1973..>2686
                     /locus_tag="PLE93_04705"
     CDS             1973..>2686
                     /locus_tag="PLE93_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH86383.1"
                     /translation="MRIASLVPSSTEMLFALGFGDQVVAVTHECDFPPEAAALPHLSE
                     TVIEPGLSPAEIDAEVKRVVGQGDPLYRLKAEVLESVRPDLIIAQDVCAVCAVSYDDV
                     LAIAARLPGNPVVLKQDPTSLVEVLGNAIEVAEATGNPEAGVALKAELDARIESVRSA
                     VSGKERPTVLALEWLDPPFTGGHWVPEMIAAAGGIDALGTEPGARSRDLSWEEVGASD
                     PDFIVVMPCGFYVTEAEVEA"
BASE COUNT          456 a          889 c          840 g          501 t
ORIGIN      
        1 cttgccgtct tccttgaagg cgggcttgag gccggccagc gcctcgaggt tggtgccggg
       61 acggatgccc tggtcggtca tgtaggtgtc accgttgacc tggaaggggg cgatttcccg
      121 ttcgaagcga ccttcttcgg tcgcctgctg ggcgcgggcg tgggaacgaa cggccagctc
      181 gtcgagatcg accttgggga tctcccactg gtcggcgatc atctcggccg agatgccctg
      241 gttgaccagg tcgtacttct cgagcagctc gggcgaatag gcgtagccgt attcgctcat
      301 caccttggcc gaatgtgcga aggagatgtg gcccatgtgc tcgacaccgc cgccgatcat
      361 cacgtcgtgg acgccggaag cgatcatcgc cgctgcgtag ttgatcgcct gctgggcgga
      421 gccgcactga cggtcgatcg tggtcgccgg ggtctcgatc gggaagccgg cctcaagcca
      481 ggcgttgcgt ccgatgttca gggtctgctc gccgaactgc tgcacgcaac cggcaactac
      541 atcctcgacc tcgcccgggt cgatcccggc ccgctcgacg acgttcttgt aggcatgggc
      601 cagaagagtc gaaggatggg tgtccttgta atagcccttc tccttgtggc cgcggccgac
      661 cggggtgcgg accgcctcca cgattacgac ttcacgaccg ccgaacttgc tcattttctg
      721 cctttcttct cctgattgat ggctggaaag aggggttgca aacttgtaac aatcgtagct
      781 gattgactcg tcagtcaatc ccggttgttg gtcgatctca atagggttca gccggatgac
      841 cgcaacaccg tatgaattcg tcgcgatcgc gggcagtggc acgatcgcca ccggcctcgc
      901 ggccgtggca tcgacctcga gcggcaaggt cgtgatgctg gttcgttccg aagagtcggc
      961 ggaccgggcc ctgaccgccg tcgaaaaggc cagccggaga atccccggag ccaacccctc
     1021 acgggtgacc acgaccatgg agatcgcgga cctggctgaa gccaacctcc tggtcgaagc
     1081 agttgtcgag gacgccgggg tcaaggcgga tctgctgaga cgaatttccg aggtcgcccc
     1141 gcgggccgat ctcgcaacca ccacttcctc cctttcgatc acgaagctgg gcgaggattc
     1201 gggggccgcc gaccgggtct acggactcca tgtcttcaac cccgtcccgg cgatgcaact
     1261 ggtcgaactg atctttcccg acccggttac cgaggaggta gccgagcgag cccacgactg
     1321 gtgccacatc ctcgaaaaga ccgccgtcga ggtgcccgac acagccggtt tcgcggtcaa
     1381 ccgtctgctc ttcccctacc tcttcgacgc agtccgctac caggagcgca ccggcctgga
     1441 ggctgccgcg gtcgaccaat gcatgaccct cggggtcgcc caccccatgg gccccctcgc
     1501 cctgctcgac ctggtcggcc tcgatgtcgc ggtagcgatc ggccaggcac tcaacaacga
     1561 atcaggaaac ccggaccaca tcgcccccgc cacggtccag gaattcgtcg ctgcaggcca
     1621 cctcggccgc aaaaccggcc agggtttcta cgaatacggc taaaccagcg cctggatcct
     1681 tgaggccagc tcgaagtctt tctcggtgag gccgccttcg gcgtggttgg ttatcgccac
     1741 ggtgaccttg ttccaggaga taccgaggtc ggggtgatgg ttcatcgatt cggccggctg
     1801 aaccagcgaa tcgacgaagt tgatcgagcc gacaaagtcg tcgaactcga acgttcgaac
     1861 gatcctccgg ccctcgaggc tccagccatc gagttcagcc aggcggtcct gtatttcctg
     1921 atcctcaagc agcgccatgg tccgagccta cccgggctgc catatttcac ccatgaggat
     1981 tgcctccctc gttccttctt ccaccgagat gctcttcgcg ctcggcttcg gcgaccaggt
     2041 cgtggccgtc acccatgagt gcgacttccc gcctgaggct gccgctctcc cccacctcag
     2101 cgagacggtt atcgagccgg gcctttcccc ggccgagatc gacgccgaag tgaagcgggt
     2161 ggtcggccag ggtgacccgc tctaccgcct caaggccgag gtactggaat cggtccggcc
     2221 ggacttgatc atcgcccagg atgtctgcgc ggtctgcgcc gtctcttacg acgatgtgct
     2281 ggcgatcgcc gcccgcctgc ccggcaatcc ggtggttctc aagcaggacc cgacctccct
     2341 ggtggaggtc ctgggcaacg cgatcgaagt ggcggaagcg accggcaacc ccgaagccgg
     2401 cgttgccctc aaggccgaac tcgatgcaag gatcgaatcg gttcgcagcg cagtgtccgg
     2461 caaggaacga ccaaccgtgc tggccctcga gtggctggac ccgcccttca ccggcggcca
     2521 ctgggtcccg gagatgatcg cggcggccgg cggcatcgac gcgctcggca ccgagccggg
     2581 agcccgctcg cgcgatctga gctgggagga ggtcggcgcg agcgatcccg acttcatcgt
     2641 cgtcatgccc tgcggcttct acgtgaccga ggccgaggtc gaggcg
//
LOCUS       DAOQFI010000216         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_19986_length_2684_cov_3.779764, whole genome shotgun sequence.
ACCESSION   DAOQFI010000216 DAOQFI010000000
VERSION     DAOQFI010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19986_length_2684_cov_3.779764"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLE93_04710"
     CDS             complement(<1..601)
                     /locus_tag="PLE93_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86384.1"
                     /translation="MAPTTAAPAATAAPSKKDIERASEFARESLLLNGDGPDSLIPGT
                     ARRRAFDQAIAELDAGLTEPSVEWRRNFSLLLGLERLIGEEPPTLKDGAELNPHQIDA
                     LSGTLTELLAEQQVSAVDDIETLEVLEEEGDTPLRDESEAEAEPSGEDDEPEDGDEAD
                     EVIDEAVDDDEEPLDWEDKADEDSGDEEEVGEAADDPGAA"
     gene            758..1351
                     /locus_tag="PLE93_04715"
     CDS             758..1351
                     /locus_tag="PLE93_04715"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH86385.1"
                     /translation="MKSAVHRHLTVLALLGALLGGLVLAGCGEEGNEPSPGPNYEKAL
                     AGAPPRLAKLYDQANELLPGGLDAFDERVQTDLAGYPAVVNIWASWCGPCRAEFPHFQ
                     QVSARMGKKLAFLGVNSDDNSDAAKTFLSTHPVPYPSYEDPDKDIAQSLGATHGLPGT
                     AFFNADGELTYTRSGPYSSDQELEADVQKYAIEGQSG"
     gene            1395..2009
                     /locus_tag="PLE93_04720"
     CDS             1395..2009
                     /locus_tag="PLE93_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNH86386.1"
                     /translation="MEISDPTLAYLLIILGLTGIGIEAITPGGFVPAILGIIALVFGV
                     IGVVDIGPTAIGLGLLLLAIALFISAIAFHLYRPLSIAGVISLVASGIWMFDRETDPT
                     SIIAVTIGAVALGLFMLFVIERASETRAAPVRYGPEDLVGMPGEVRVPLRPDGQVFVD
                     GALWQARLADSTARLGVGEKVTVTEVSGLTLLVAPASETNQGEI"
     gene            2012..>2684
                     /locus_tag="PLE93_04725"
     CDS             2012..>2684
                     /locus_tag="PLE93_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNH86387.1"
                     /translation="MAVVGTLVVILAIFALIVLFSAIKILREYERGVIFRLGRLINQK
                     GPGLILLIPFIDRMVKIDLRTVTLNIPPQEVITRDNVPTSVNAVCYFRVVDPNRAITD
                     VENYLIATSQIAQTSLRAVLGKAELDEILSERERLNESLQKIIDEQTEPWGVKVTTVE
                     IKDVEIPEAMQRAMARQAEAERERRAKIINSEGEYQAAEKLTDAADIISRNPASLQLR
                     YLQTLL"
BASE COUNT          459 a          905 c          834 g          486 t
ORIGIN      
        1 gggcggcacc gggatcgtcg gcagcttcgc cgacttcctc ttcatccccg gaatcctcgt
       61 ctgccttgtc ctcccagtcc aggggctcct cgtcatcgtc cacggcctcg tcgatgacct
      121 catcggcctc gtccccgtct tcgggctcgt catcctctcc ggaaggttcg gcttcggctt
      181 cgctctcgtc ccggagcggg gtgtcgcctt cctcctcgag aacttccagg gtctcgatgt
      241 catcgaccgc actcacctgc tgctcggcca gcagttcggt cagggtgccg gaaagggcat
      301 cgatctgatg cgggttcagc tcggccccgt ccttcagggt cggcggttcc tcgccgatca
      361 ggcgttcgag cccgagcagc agcgaaaagt tccggcgcca ctcgaccgaa ggttcggtca
      421 ggccggcatc gagttcggcg atcgcctggt caaaggcacg gcggcgggcg gtacccggaa
      481 tcagcgaatc gggaccgtcc ccgttcagca gcagggattc acgcgcaaat tcagaagcgc
      541 gctcgatgtc tttctttgag ggagccgcag tagctgcggg tgctgcagta gtcggggcca
      601 acttagcctt cgcaagtgtt ccgaacccgg attctcgacc cggcccggat gagtaggtga
      661 tcgaagattc cgacgacgac gctggacgga cagacgccgg tttcctccgt gatccgcgat
      721 acggcggatc gaatgtctgt atttaatcat tacttgcatg aaatcggctg ttcacagaca
      781 tctgacggtt cttgccctgc tcggcgcgct gctcggcggg ctggtcctgg ccggttgcgg
      841 tgaagaaggg aacgagccca gccccgggcc gaactacgag aaggccctgg ccggggcacc
      901 acctcgcctg gcgaagcttt acgaccaggc aaacgagctc ctgccgggcg gactcgacgc
      961 cttcgacgag cgggtccaga ccgacctggc cggctatccg gcggtggtca acatctgggc
     1021 ttcctggtgc ggaccctgcc gggccgagtt tccacacttc cagcaggtct cggccaggat
     1081 gggcaaaaag ctcgccttcc tcggcgtgaa ctcggatgac aactcggacg cggcgaagac
     1141 tttcctctcg acccatccgg tcccctaccc cagctacgag gacccggaca aggacatcgc
     1201 ccagtccctc ggggccaccc atggcctgcc cgggacggcg ttcttcaacg cagacgggga
     1261 actgacctac acccgttccg gcccctactc cagcgaccag gagctggaag cagacgtcca
     1321 gaagtacgcg atcgagggtc agtccggctg agcccgctta ctgcccaccg ggcaggaccg
     1381 atacgctcgg gtccgtggag atcagtgatc cgacactcgc ctacctgctg atcattctcg
     1441 gcctgaccgg gatcgggatc gaggcgatca cgcccggcgg tttcgtgccg gcgattctcg
     1501 gcatcatcgc tctggtcttc ggggtgatcg gagtggtcga catcgggccg acggcgatcg
     1561 ggctcggcct gctgctgctg gcgatcgccc tgttcatctc cgcgatcgcc ttccacctct
     1621 accggcccct ctcgatcgcc ggagtgattt cactggtcgc ctcgggcatc tggatgttcg
     1681 accgtgagac cgacccgacc tcgatcatcg cggtgacgat cggcgcggtt gcgctcggac
     1741 tcttcatgct tttcgtgatc gagcgggcca gtgaaaccag ggcagccccg gtccgttacg
     1801 gcccggagga cctggtcggg atgccgggtg aagtccgggt gccgctccgg ccggatggcc
     1861 aggtcttcgt tgacggcgca ctctggcagg cccgcctcgc cgactccaca gcccggctcg
     1921 gcgtcggcga gaaggtcacc gtcaccgagg tcagcggcct gacccttctg gtcgcccccg
     1981 cctcagaaac caaccaagga gagatctaga gatggcagtc gtcggaaccc tggtcgtgat
     2041 cctcgcgatc ttcgccctga tcgtcctgtt ttcggcgatc aagatcctgc gcgaatacga
     2101 gcgcggcgtg atcttccggc tcggccggct gatcaaccag aagggtcccg ggctgatcct
     2161 gctgatcccc ttcatcgacc gcatggtcaa gatcgacctg cgaaccgtga ccctgaacat
     2221 cccgccgcag gaagtgatca cccgcgacaa cgtgcccacc tcggtcaacg cggtctgcta
     2281 cttccgcgtg gtcgacccca accgggcgat caccgatgtc gagaactacc tgatcgccac
     2341 ctcgcagatc gcccagacct cgctccgggc ggtgctcggc aaggctgaac tggatgagat
     2401 cctttccgag cgagagcgcc tcaacgagtc gctgcagaag atcatcgacg agcagaccga
     2461 gccctggggc gtgaaggtca cgacggtcga gatcaaggac gtcgagattc ccgaggcgat
     2521 gcagcgggcg atggcccggc aggccgaggc cgaacgggaa cggcgggcca agatcatcaa
     2581 ctccgaaggc gagtaccagg cggcggagaa gctcaccgac gcggccgaca tcatcagccg
     2641 caacccggct tcgctgcagc tgcgctacct gcagaccctg ctcg
//
LOCUS       DAOQFI010000217         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20064_length_2678_cov_2.602745, whole genome shotgun sequence.
ACCESSION   DAOQFI010000217 DAOQFI010000000
VERSION     DAOQFI010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20064_length_2678_cov_2.602745"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLE93_04730"
     CDS             complement(<1..452)
                     /locus_tag="PLE93_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HNH86388.1"
                     /translation="MATADTETIGYTGGPQAWTVPLGVHSVEFDLYGAAGSNNGNSGF
                     VGGKGGRAKATFDVSPGQLMRLYIGGTGGFLAGGWNGGGHGFAGGGGGTDLRIGGTGL
                     GNRVLVAGGGGGVGNYGGASRGGDGGGLVGTSGTSPTAGAPGTQTEGGA"
     gene            613..861
                     /locus_tag="PLE93_04735"
     CDS             613..861
                     /locus_tag="PLE93_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNH86389.1"
                     /translation="MATFSISKARESLSDAVERSRTEAVFLERHGKKAAVMVSPERYE
                     EMLEAFEEIEDVAAFDQAMAEESPNIPWDEAKADLGWD"
     gene            858..1118
                     /locus_tag="PLE93_04740"
     CDS             858..1118
                     /locus_tag="PLE93_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNH86390.1"
                     /translation="MSRYRIELRPAALRSLRKLDPQIRRRVQGAIALLADDPRPPATR
                     KLKGRDAFRVRVDDYRVIYTIEDEVLLIVVVTLGHRRDVYER"
     gene            1369..2355
                     /locus_tag="PLE93_04745"
     CDS             1369..2355
                     /locus_tag="PLE93_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86391.1"
                     /translation="MSVKFRGFLGLLLAAVVLSTGHADAAKNGPILFSDYTSIWKIKA
                     DGSGLKRVAKKKAWSLHASPNGKQLLYTHDGLFRMPINGGRSVNLLKRYPIVDRFAGV
                     RWASWSPNGKQIAFEGGNDGRIYLIKSNGRGLRYLFRKYRTGLLHPVWSPNGKEIAYI
                     DVHNNSSLMAVNLKSGKKRTIYPGTGEAGTPVSFDWHPSGKRIAFYAPYRNWMIDSDG
                     SDLRQISPDQAFVSYEDLVFSPDGKSLLGRAYSGESSTSELWLLDGFYGAASGGFVNE
                     ITGRFGGSPFSPEWAPRSRVSRRARPRGLPGIRGGTFRRACRGFHRRRSATQ"
     gene            complement(2255..>2678)
                     /locus_tag="PLE93_04750"
     CDS             complement(2255..>2678)
                     /locus_tag="PLE93_04750"
                     /inference="COORDINATES: protein motif:HMM:NF021568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HNH86392.1"
                     /translation="LPSQSFGAARMTGADPGQATGHRKIAAEISDGMIELIREFYGTE
                     PSRTSTIYQDDLVLVVIVGGFTTIEKTLSEAGRTEEVSRLRHAFQDVMESRVKALVEN
                     ATGRKVTALLSGDDENPDMLGEMFLLESREGPEDEPAG"
BASE COUNT          492 a          863 c          838 g          485 t
ORIGIN      
        1 gctccgcctt cggtctgggt gccgggggca ccggcggtcg gtgaggtgcc gctcgtgccg
       61 acgagtccgc cgccatcgcc gccgcgagag gcaccgccgt aatttccgac accgccaccg
      121 ccaccggcca caagcactct gttgcccagt ccggttccgc cgatcctgag gtctgtcccg
      181 ccgcccccgc cggcaaatcc gtgaccgccc ccgttccagc caccggcaag aaagccgccg
      241 gttccgccga tgtacagacg catgagctgg cccgggctga cgtcgaaggt cgccttcgcg
      301 cgaccgccct tgccaccgac gaagccgctg ttgccgttgt tgcttcccgc cgctccgtag
      361 agatcgaact cgaccgagtg cacgccgagc gggacagtcc aggcctgcgg gccacctgtg
      421 tagccgatcg tctcggtgtc ggcggtggcc aaagtcgggg acccggcaca caagagagcg
      481 gcgatcaacc ccacaagtcc gagccgccat cccacccgca gtagaagtgc gcttctaacc
      541 ggtcggctca tatggagaca ccctatccgg agtgttctgt acaaattgga ttcggaactg
      601 tacaatttca gcatggccac tttcagcatc agcaaagcga gggagtcact ttcggacgcg
      661 gtcgagcggt cccggaccga agccgtcttt ctcgagcggc atggcaagaa ggctgcggtg
      721 atggtcagtc ccgagcgcta cgaagagatg ttggaagcgt ttgaggagat cgaagatgtc
      781 gctgcctttg atcaggcgat ggccgaagaa agtccgaaca tcccctggga cgaagccaag
      841 gccgacctgg gttgggattg agccgctatc ggatagagct gcgcccggcc gcgctccggt
      901 ccctgcgcaa actagacccc cagatcagac ggagagtcca aggagcgatc gctctccttg
      961 ccgacgaccc tcgcccgcca gctaccagga aactgaaagg acgagacgcc ttccgggtcc
     1021 gggtggacga ctaccgggtg atctacacca ttgaagacga ggtgctgttg atcgttgtgg
     1081 ttacgctcgg gcaccggcgg gacgtctacg agcggtagat actgccgctg ttgccgtcgc
     1141 cttagagggc gagccagggg cgtgctgggc cggacgcctc actccgcgaa gcggttggtc
     1201 ttttgtgccc gagcgggaac cacgcagggt gaaagccgct gggatccggc ctgccgcgcc
     1261 tgggcgatcc ataccccggg ctcgtactcg taaacgactg ccagggattc gcgtgcctat
     1321 acttgcggcg gtccgggggg tccagtcgaa cgagcaggga gaagtgccat gagtgtgaag
     1381 ttccgcggtt tcctcggatt gctgcttgcc gcagtcgtcc tttcaaccgg tcacgccgac
     1441 gcggcgaaga acgggccgat cctgttcagt gattacacct cgatctggaa gatcaaggca
     1501 gacggttcgg gcctcaagcg ggtggcgaag aagaaggcct ggagtcttca cgcctctccc
     1561 aacggcaaac agctcctcta cacacatgac ggcctcttcc ggatgccgat aaacggcggc
     1621 aggtcggtca acctgctcaa gcgctacccg atcgtcgacc ggttcgccgg tgtcagatgg
     1681 gcttcctggt cgccgaacgg aaagcagatc gctttcgagg gcggcaacga cggccgcatc
     1741 tatctgatca agtcgaacgg ccgcggcctg aggtacctgt tccgcaagta ccggaccggc
     1801 ctgctccacc ccgtctggtc cccgaacggg aaggaaatcg cctacatcga cgtccacaac
     1861 aactcctcgc taatggcggt gaacctcaag tcgggtaaga agcgaaccat ctacccgggc
     1921 accggtgaag ccgggacgcc ggtcagtttc gactggcacc cgagcggcaa gcggatcgcc
     1981 ttctacgccc cgtaccggaa ctggatgatc gacagcgacg gcagcgacct gcggcagatc
     2041 tcacccgacc aggccttcgt ctcctatgag gacctcgtct tctcgccgga cgggaagtca
     2101 cttctcggac gggcatattc gggtgaaagc tcgaccagcg aactctggct gcttgacggg
     2161 ttctacggcg ccgcgagcgg tggttttgtg aacgagatca ccggccgctt cggcggcagt
     2221 ccgttctccc cggagtgggc cccgcgtagc cgggttagcc ggcgggctcg tcctcggggc
     2281 cttcccggga ttcgaggagg aacatttcgc cgagcatgtc ggggttttca tcgtcgccgc
     2341 tcagcaacgc agtgactttt cttcccgtgg cattttcaac cagggccttg accctcgact
     2401 ccatgacgtc ctggaacgcg tgacgaagcc gggatacctc ctcggttcga cccgcctcgc
     2461 tcagggtctt ttcgatcgtc gtgaagcccc cgacgatcac caccagcaca aggtcgtcct
     2521 ggtagatggt gctcgtccgt gaaggctcgg tgccgtagaa ctccctgatc agttcgatca
     2581 tgccgtccga gatctcggcg gcgatctttc gatgtccggt ggcttggcct ggatcagcgc
     2641 cggtcatccg ggcggccccg aaagactgcg aaggcaag
//
LOCUS       DAOQFI010000218         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20162_length_2670_cov_3.157935, whole genome shotgun sequence.
ACCESSION   DAOQFI010000218 DAOQFI010000000
VERSION     DAOQFI010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20162_length_2670_cov_3.157935"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLE93_04755"
     CDS             <1..737
                     /locus_tag="PLE93_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HNH86393.1"
                     /translation="ARHTCALYKINRETGKVMWRMGGKDSDFKLASGFRFCYQHDFRP
                     HPNGTYSIFENGAADGELKPKARMSKAYIFRINQKKKTTALVKTYIHPGNLLSPSQGN
                     TQLLPNGNVFTGWGSLEYCTEFAPDGEVLWDMRYSAAAVTYRCYRSPWTGTPVPGAIG
                     VKSETDGPNSRVWVSWNGDTQVRTWRVLAGNPGNLAYSTEVPRDGFETSIPVTGQPAA
                     FRLVGLDGQGKVLGRSRVNKLGELTR"
     gene            734..2536
                     /locus_tag="PLE93_04760"
     CDS             734..2536
                     /locus_tag="PLE93_04760"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HNH86394.1"
                     /translation="MNLALPNRSRRAAGSAAAALAAALAVLAAALALAAPAGAKPIAA
                     YPSPGSVYASPTTNIALSGVTRASVGRIMVRGSQSGFHRGRIEAWAGPVGVSFIPSRP
                     FAPLEKVTVTSRSHPFYGTGGARSYSFKTGEFLPENLGADPFSPAKGQTPRASQTYKT
                     LRLKVPKIVVHANEPGKSNGKIFYAPRTSGPTILDADGNLVWYRPGLRITDFRAQVYN
                     GHRILTWWRRDTFGKRVTSRFEMANRHYKVFRRFGGGNGFTGDPHEFNLTSRGTAFVT
                     AYKTAVVDLSRFGGPRRAFLLDYIGQEIDIKTGLVVWEWHPLGNLPMNRTYLPIPRRN
                     TRPFDWFHMNSINDDNDGNVLISARHTQALYKINRKTGRIMWQIGGKGGDFKLGKGVR
                     FGFQHDLIRQKNGTLTIFDNGAGGVHGKVNRFSSAKVLRVNAKRRRVTLVRAYRDPRN
                     VISNSQGNTDVQANGNIFVGWGDRNACTEFAPDGRVLFDFTFAARTVSYRCFKRPWSG
                     APTTPVAVKSERESDGSQVWMSWNGDTRVAEWRVLAGTAPGKLVEITTVPRDGFESTA
                     TLDQAFKYYRAVGLSAGGKLLGRSELNRLGRLTD"
BASE COUNT          486 a          976 c          819 g          389 t
ORIGIN      
        1 cggcccgtca cacctgcgcg ctgtacaaga tcaaccgcga gaccggcaag gtcatgtggc
       61 gaatgggcgg caaggactcg gacttcaaac tggccagcgg tttccgcttc tgttaccagc
      121 atgacttccg gccccacccc aacggcacct actcgatctt cgagaacggc gccgccgatg
      181 gcgaactgaa gccgaaggcc aggatgtcga aggcctacat cttccggatc aaccagaaaa
      241 agaagaccac cgccctggtc aagacctaca tccaccccgg caacctgctc tcaccgagcc
      301 agggcaacac gcaactcctg ccgaacggca acgtcttcac cggctggggc tcgctcgagt
      361 actgcaccga gttcgccccc gacggcgagg tgctctggga catgcggtat tcggccgctg
      421 ccgttaccta ccgctgctac cggtcaccct ggaccggcac cccggtgccc ggcgcgatcg
      481 gagtcaagag tgaaaccgac ggcccgaaca gccgggtctg ggtgagctgg aacggtgaca
      541 cccaggtcag gacctggcgg gtcctggccg gcaacccggg caacctcgcc tactcgaccg
      601 aagtaccccg cgacgggttc gaaacctcga tcccggtgac cggccagccg gccgcgttcc
      661 gactcgtcgg actcgacggt caaggcaagg tgctgggccg gtccagggtc aacaagctcg
      721 gagagctcac ccggtgaacc tggccctccc gaaccgcagc cgccgggcgg ccggatcagc
      781 agcggccgct cttgcggcag ccctcgcggt cctggccgcc gcgcttgccc ttgccgcacc
      841 ggccggggcg aagccgatcg cggcctaccc cagccccggc tccgtctacg cctccccgac
      901 cacgaacatc gcgctgagcg gcgtcacccg ggccagcgtc ggccggatca tggtccgcgg
      961 ctcgcagtcc ggcttccatc gcggccggat cgaagcctgg gcgggcccgg tcggggtcag
     1021 cttcatcccc agccggccct tcgcgccgct ggagaaggtc accgtgacct cgcgcagtca
     1081 ccccttctac ggcaccggcg gcgcccgctc atactcgttc aagaccggcg agttcctgcc
     1141 ggaaaacctg ggggccgacc cgttcagccc ggccaagggg cagacccccc gcgcctcgca
     1201 gacctacaag acactgcgac tcaaagtgcc gaagatcgtc gtccacgcga acgagccggg
     1261 caaatccaac ggcaagatct tctatgcccc gcggaccagc ggcccgacca ttctcgacgc
     1321 cgatggcaac ctcgtctggt accgccccgg cctgcggatc accgacttcc gggcccaggt
     1381 ctacaacggc caccggatcc tcacctggtg gcgtcgcgac accttcggca agcgcgtcac
     1441 cagcaggttc gagatggcca accgccacta caaggtcttc cgccgcttcg gcggaggcaa
     1501 cggcttcaca ggcgacccgc atgagttcaa cctgacttcc cgcggcaccg ccttcgtcac
     1561 cgcctacaag acggccgtcg ttgacctgag caggttcggc gggccgcgtc gcgccttcct
     1621 gctcgattac atcggccagg aaatcgacat caagaccggt ctcgtggtct gggaatggca
     1681 cccgctcggc aacctcccga tgaaccggac ctacctgccg atcccgcgac gcaacacccg
     1741 tcccttcgac tggttccaca tgaactcgat caacgacgac aatgacggca acgtcctgat
     1801 ctccgcccgc cacacccagg ccctctacaa gatcaaccgc aagacgggtc ggatcatgtg
     1861 gcagatcggc ggcaagggcg gggacttcaa actcggcaag ggggttcggt tcgggttcca
     1921 gcatgacctg atccgccaga agaacggcac cctgacgatc ttcgacaacg gcgctggcgg
     1981 ggttcacggc aaggtcaacc ggttctccag cgccaaggtg ctcagggtca acgccaagcg
     2041 gcgccgggtc accctggtcc gcgcctaccg cgacccccgc aacgtgattt ccaacagcca
     2101 gggcaacacc gacgtccagg caaacggcaa catcttcgtc ggctggggtg accggaacgc
     2161 ctgcaccgag ttcgcgccgg acgggagggt cctcttcgac ttcaccttcg ccgccaggac
     2221 ggtttcctac cgttgcttca agcggccctg gagcggggcg ccgaccaccc cggtcgcggt
     2281 caagagcgag cgcgagagtg acggcagcca ggtctggatg agctggaatg gcgacacccg
     2341 cgtcgccgag tggcgggtgc tggccggcac tgctcccggc aaactggtcg agatcacgac
     2401 cgtgccccgg gacggattcg aaagcaccgc aacccttgac caggccttca agtactaccg
     2461 ggcggtcggc ctctcggccg gtggaaagtt gctcggccgc tcggaactca accggctcgg
     2521 ccggctgacc gactagctcc gcgacggccg gggcggctcc ggcaggggtg cccggcagac
     2581 accggcctgg cattcggtga cccggttcag gcgggcccgc atccaggcga gcacgccgga
     2641 ataggccggt gccccggccc ggttgttcat
//
LOCUS       DAOQFI010000219         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20192_length_2668_cov_3.034826, whole genome shotgun sequence.
ACCESSION   DAOQFI010000219 DAOQFI010000000
VERSION     DAOQFI010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20192_length_2668_cov_3.034826"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLE93_04765"
     CDS             <1..924
                     /locus_tag="PLE93_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86395.1"
                     /translation="LSWRGPFSGWGNSVTPGPGFNPVANLSSFLSETRTAFDTGRQQE
                     EIAESRDELRSRYGLGPRLRAELGERPASVEPWEVMAAWAYELDWSPAPVFQNYSAYT
                     SELDQLNAGVISSDSGPERILRHFGRNPDYPERGIDQRLLAWDPPAQAVATFCHFRPV
                     ATSFEWQVLARTGNRCGPMVPAGSVDSSFGQSVAVPPPGPGEVVLVRIHGAGVHGLER
                     LRSLVFRPKERRVQAGRGSYRLVPGTAGDGLMLRAGPGVPDASGRFSQVPRIRRLELT
                     GTSGDLRFDFFRMKVRPSRAAPRAAVRLPTQ"
     gene            921..>2668
                     /locus_tag="PLE93_04770"
     CDS             921..>2668
                     /locus_tag="PLE93_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86396.1"
                     /translation="MNGFESKLRSWLEAIGRWNPPSWIPGLMIALLGWTVGFAHPFGG
                     LDSSWWVGLYLAAHNQMQFGTEIVFTYGPLGILRFPWLLYPGALAVIPFVYASILHVG
                     FSSALVAALRRRVGLLWAMLVALLVVVQLEWVEQEVAISILAAMLMIERRPAGRKLLF
                     FAVCAGLLAGTEMLVKLSTGPVIAAVLLVGLVGARARTREIAAYLGTLAGSMLVCWFV
                     TGQDLSNLPDFIGNSIQIVLGYQEAMAVYGQSGWYSLIIVAGGALCFAWALFGGYADR
                     RARIAGALVAAIVIFVFYKQAVVRIDSAHIALFFALIAVLWLAVPPRQGFAGISLAGL
                     AFMGVVSIYAATGVPSPGLNLISNLRGFYEESRTALSPARQRAGVDFWRQGIRHNYQL
                     DPKLLAELEGQSVSVEPWETTAAWAFELDWHPAPVFQNYSAYTSALDELNSDTIESAT
                     GPGRILRHVGDNPFGPTIGLDGRFMAWDPPGQAIAELCNFRPIGESGRWQVLARTANR
                     CGPLVPAGVVESRFGETVPVPKPGPNEVVIAKIHGAEVHGLERLRSFFYRPAERRAVV
                     EGGSYRLIAATAADGL"
BASE COUNT          359 a          907 c          926 g          476 t
ORIGIN      
        1 ctgtcctggc gcggcccctt ctccggctgg gggaacagcg tcacccccgg cccgggcttc
       61 aacccggttg ccaacctgag ctcgtttctg agcgaaacga gaaccgcttt cgacaccggc
      121 cgccagcagg aggagatcgc cgaaagccgc gatgagctcc ggtcccgtta cggtctcggc
      181 ccccggctgc gggccgaact cggcgagcgg ccggccagcg tcgaaccctg ggaggtgatg
      241 gcggcctggg cctatgagct cgattggtcg ccggcgccgg tcttccagaa ttacagcgcc
      301 tacacctcgg agctggacca gctgaacgcc ggggtgatca gctcggattc cgggcccgag
      361 cggatccttc gccatttcgg ccggaacccc gactaccccg aacgggggat cgatcaacgc
      421 ctgctggcct gggatccacc ggcccaggcg gtggcgacct tctgtcactt ccgcccggtc
      481 gccacctcgt tcgagtggca ggtgctggcc aggaccggca accgctgcgg tccgatggtc
      541 ccggccggca gcgtggattc cagcttcggc cagagcgtgg ccgtgccgcc gcccggcccc
      601 ggcgaggtgg tgctggtccg gattcacggg gccggggtcc acggtctgga gcgcctgcgc
      661 tcgctggtct ttcggccgaa ggagcggcgg gtccaggcgg ggcgcggctc ctaccgcctg
      721 gttcccggga ccgccggaga cgggctgatg ctccgggccg gacccggggt ccccgacgcg
      781 tccgggcgct tctcgcaggt tccccggatt cgccgtctcg aactgaccgg cacctccggc
      841 gatctcaggt tcgacttctt ccggatgaag gtccggccct cgcgtgcggc gccgcgggca
      901 gcggttaggc tgccgaccca gtgaacggat tcgagtccaa gcttcggagc tggctggagg
      961 cgatcgggag gtggaatccg cccagctgga ttcccgggct gatgattgcc ctgctcggct
     1021 ggacggtcgg tttcgcacat cccttcggtg gtctcgactc gagctggtgg gtcggtctct
     1081 acctcgcggc ccacaaccag atgcagttcg ggaccgagat cgtcttcacc tacggtccgc
     1141 tcggaatcct ccgtttcccc tggctgctct acccgggcgc gctggcggtg atcccgttcg
     1201 tttacgcatc gatcctgcat gtcggtttca gctcggccct ggttgcggca ctgaggagaa
     1261 gggtcggtct gctctgggcg atgctggtcg ccctgctcgt ggtggtccag ctcgagtggg
     1321 tcgagcagga ggtggcgatc agcatcctgg cggcgatgct gatgatcgag cggcgcccgg
     1381 ccggccgcaa gctgctcttc tttgcggtct gtgccggatt gctggccggg accgaaatgc
     1441 tggtcaagct ttcgaccggc ccggtgatcg ccgcggtcct cctggtcgga ctggtcgggg
     1501 cgcgggcgcg gacccgcgag atcgcggcct acctcggcac cctggccggg tcgatgctgg
     1561 tctgctggtt cgtgaccggc caggacctct cgaacctgcc cgacttcatc ggcaactcga
     1621 tccagatcgt gctcggctat caggaggcga tggcggtcta cggccagtcc ggctggtact
     1681 cgctgatcat cgtcgccggc ggagcgctct gtttcgcctg ggccctgttc ggcggttacg
     1741 ccgaccgccg ggcccggatc gccggtgccc tggtcgcggc gatcgtgatc ttcgtcttct
     1801 acaagcaggc ggtggtgcgg atcgacagcg cccacatcgc cctgttcttc gccctgatcg
     1861 ccgtgctctg gctggccgtc ccgccgcgac aggggttcgc cggaatctcg ctggccggac
     1921 tggccttcat gggtgtggtt tcgatctatg cggcgaccgg cgtgccttcc cccgggctga
     1981 acctgatttc caacctgagg ggtttctacg aggaaagcag gacggccctc agcccggccc
     2041 gtcagcgggc cggcgtcgac ttctggcgcc agggaatccg gcacaactac cagctcgacc
     2101 cgaaactgct ggccgagctc gaaggccaga gcgtcagcgt cgagccctgg gagaccaccg
     2161 ccgcctgggc cttcgagctc gattggcacc cggccccggt cttccagaac tacagcgcct
     2221 acacctcggc gctggatgag ctgaattcag acacgatcga gtcggccacc gggcccggcc
     2281 gaatcctgcg gcatgtcggt gacaatccct tcgggccgac gatcggcctc gacggaaggt
     2341 tcatggcctg ggacccgccc ggccaggcga tcgcggaact ctgcaacttc aggccgatcg
     2401 gcgagtcggg acgctggcag gtgctggccc gcaccgccaa ccgctgcggc ccgctggtcc
     2461 cggccggggt ggtggagtcc cggttcggtg aaacggtccc ggtgccgaag cccggcccga
     2521 acgaggtagt gatcgcgaag atccacggag ccgaggtcca cgggctggaa aggctgaggt
     2581 cattctttta ccggccggcc gagcgccggg cggtggtcga gggaggctcc tatcgcctga
     2641 tcgccgccac cgcggcggac gggctgat
//
LOCUS       DAOQFI010000220         2663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20260_length_2663_cov_2.934816, whole genome shotgun sequence.
ACCESSION   DAOQFI010000220 DAOQFI010000000
VERSION     DAOQFI010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20260_length_2663_cov_2.934816"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PLE93_04775"
     CDS             <1..1264
                     /locus_tag="PLE93_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86397.1"
                     /translation="FLACAVLYLISLPWVGDYSLAKALVIAAPVTMVVILTALLSGPR
                     GGWKPSQGMDFGAWVTLATLFVIGAAASSLIVLRDASVSPPGHAKELGAFKREVKGST
                     VLYADQDRFAPYYLTGAKVSVPLADFPDPDIVENPKKPFQGDTGQGVIDFDSFEGESL
                     QNFDYVITSSASFQSEAPAFFEEVDRTNSYILWKRIDDAYNRPILNEATLPGRLVDCS
                     KGGGQYFSTEVDGTATLMPRTVLALRDQWTPSPDLHQGDSASLTLDLTPGFWWISMQY
                     FTPMGFKLTTDQGFERFFKPALDGQRLVNQETGSNGQYWPAGVIEVKKAGPVTFTAKS
                     AEPTAIQRLSGYSRITKLGRVAAMRGQGRWKVAMNQICGQWVDWFKRIPISIFRQNDT
                     RQKEAARRQTLREAARFNRVEGSGAVGD"
     gene            1369..>2663
                     /gene="zwf"
                     /locus_tag="PLE93_04780"
     CDS             1369..>2663
                     /gene="zwf"
                     /locus_tag="PLE93_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNH86398.1"
                     /translation="MADIGPLDVAPTALAPPEPHVVVLFGATGDLSRRKLLPGLFHLD
                     RAGHLSDCRIVGTSPEEMTNAGFRRLARDSCESFGRGRVEEDDWNRFERRLSWVSGTT
                     GAAGLSDAVDRAEADIGEQCRRLHYLSVPPAAAPAVIKTLGEAGLVERSRIIMEKPFG
                     TDIESARELNAMVHEVFDEDQVFRIDHFLGKEAALNILALRFANGLFEPIWSRNFIDH
                     VQIDVPETLTVEGRGGFYDATGAFRDMVVTHLFQVLAFVAMEPPVELAPDAITVEKNK
                     VFRSMKGIRPEDVVRGRYEGYHQESGVAEDSDAETFIALKCGIDNWRWSGVPFYLRTG
                     KAMSEGARIISIAFREPPKSMFPEGSGVGMKGPDHLTFDLDESSRISLSYYGKRPGPG
                     MLVDKLSMQFSLNEIETQGDTLEAYERLIRDAMGGDHTL"
BASE COUNT          477 a          875 c          860 g          451 t
ORIGIN      
        1 cttcctcgcc tgcgccgtgc tctacctgat ctcgctgccc tgggtcgggg actattcgct
       61 ggccaaggcc ctggtcatcg ccgccccggt gaccatggtg gtaatcctga cggcgctgct
      121 gagcggcccg agagggggct ggaagcccag ccagggaatg gacttcggcg cctgggtgac
      181 cctcgccacc ctgttcgtga tcggcgccgc ggcctcgagc ctgatcgtgc ttcgggatgc
      241 ctcggtttca ccacccggcc acgcgaagga actcggcgcc ttcaagcggg aggtgaaggg
      301 cagcaccgtc ctctacgccg atcaggatcg cttcgccccc tattacctga ccggggccaa
      361 ggtctcggtg ccgctggccg acttccccga ccccgacatc gtcgagaacc cgaagaagcc
      421 gtttcagggc gacaccggcc agggcgtgat cgacttcgat tcgttcgagg gcgaaagcct
      481 ccagaacttc gactacgtga tcacctcctc ggcttccttc cagagcgagg caccggcctt
      541 cttcgaagaa gtcgaccgga ccaactcgta catcctctgg aaacggatcg acgatgccta
      601 caaccggccg atcctgaacg aggcgaccct gcccgggcgc ctggtcgact gctcgaaggg
      661 gggcggccag tatttctcga ccgaagtcga cggcaccgcg accctgatgc cccggaccgt
      721 actggccttg cgggaccaat ggacgccctc ccccgatctg caccagggcg attcagccag
      781 tctgaccctg gacctcaccc cgggattctg gtggatctcg atgcagtact tcaccccgat
      841 gggtttcaag ctgaccaccg accagggctt cgaacggttc ttcaagccgg cgcttgacgg
      901 tcagcgcctg gtcaaccagg aaaccggcag caacggccag tactggccgg ccggggtgat
      961 cgaagtgaag aaggccggcc cggtcacctt cacggccaag tcagcggagc cgacggcaat
     1021 tcagcggctc agcggctact cgaggatcac caaactcggt cgggtcgccg cgatgcgcgg
     1081 ccagggccgc tggaaggtgg cgatgaacca gatctgcggc cagtgggttg actggttcaa
     1141 gcgcattccg atttccatct tccgccagaa cgacaccagg cagaaggaag ccgcacgccg
     1201 gcagaccctg cgtgaagcgg cgcgcttcaa ccgggtcgag ggcagcggag cggtcggcga
     1261 ctgagctctt ccggccagcg gcagggttgg accatggtcg cagcgccggc ctgaaaaaca
     1321 ggacaggaag ttagccattg gtcaaccggg taccggatag attcgagcat ggccgacatc
     1381 ggaccccttg acgttgcccc gaccgcgctg gccccgccgg aaccccatgt ggtggttctc
     1441 ttcggggcca ccggcgacct ttcccgacgc aagctgctgc ccggcctctt tcatctcgac
     1501 cgggccgggc atctgtccga ttgccggatc gtcggcacct caccggagga gatgaccaat
     1561 gccgggttcc ggcggctcgc ccgggattca tgtgagtcct ttggccgggg ccgggtcgaa
     1621 gaggatgact ggaaccgttt tgaacgccgg ctctcctggg tgagcggaac caccggcgcg
     1681 gccggtctga gcgatgcggt ggacagggcg gaggcagaca tcggcgagca atgtcgccgg
     1741 ctccattacc tgagcgtgcc gccggccgcg gcgccggcgg tgatcaagac cctgggcgag
     1801 gccggcctgg tcgagcgttc gcggatcatc atggaaaagc ccttcggcac cgacatcgag
     1861 tcggcccgtg agctgaacgc gatggtccac gaggtcttcg atgaggatca ggtcttccgc
     1921 atcgatcact tcctcggcaa ggaagcggcg ctgaacatcc tcgccctgcg cttcgccaac
     1981 gggctcttcg agccgatctg gagccggaac ttcatcgacc acgtccagat cgacgtgccc
     2041 gagacgctga ccgtcgaggg gcggggcggc ttctacgacg cgaccggcgc cttccgggac
     2101 atggtggtca cccatctctt ccaggtcctc gctttcgtcg cgatggagcc gccggtcgag
     2161 ctggccccgg acgcgatcac cgtggagaag aacaaggtct ttcgctcgat gaagggcatc
     2221 cggccggaag acgtggtccg gggccgctac gagggatacc accaggaaag cggggtcgcc
     2281 gaggattccg acgccgagac attcatcgcc ctgaagtgcg ggatcgacaa ctggcgctgg
     2341 tccggggttc ccttctacct gcgcaccggc aaggcgatgt ccgaaggggc acgaatcatc
     2401 tctatcgcct tccgcgaacc tcccaagagc atgttcccgg agggctcggg ggtcgggatg
     2461 aagggcccgg accatctcac cttcgacctc gacgagtctt cgcggatctc cctctcctac
     2521 tacggcaagc gacccgggcc gggcatgctg gtcgacaagc tgagcatgca gttttcgctg
     2581 aacgagatcg aaacccaggg agacacgctg gaggcctacg aaaggctgat ccgcgatgcg
     2641 atgggtggtg accacacgct ctt
//
LOCUS       DAOQFI010000221         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20343_length_2657_cov_3.160646, whole genome shotgun sequence.
ACCESSION   DAOQFI010000221 DAOQFI010000000
VERSION     DAOQFI010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20343_length_2657_cov_3.160646"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLE93_04785"
     CDS             <1..242
                     /locus_tag="PLE93_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HNH86399.1"
                     /translation="KRLSWKPRHPLHLVLLRVHRIPRPVTVRVREEYHGCRSWVEIDR
                     DLPFEGTPVMSDEEFEFATNEIRSICAGDREPALV"
     gene            365..1174
                     /locus_tag="PLE93_04790"
     CDS             365..1174
                     /locus_tag="PLE93_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86400.1"
                     /translation="MTWIETTSLTFTARHEEGDRSYAEELLDRLEDLSLKLEDRFEVV
                     PGDISIVIHPSPVWLTAAHPFLPFVRYAAAPAGRRYLAGWPMATEVHVLGEKAMEKRA
                     AGEASAEALRGTAERLYTQIVLAANNERIPPPWTPMRFLRYLNWAWLVEGSAQHFARQ
                     VDFFRPAVIRRLREGSRPSFPPSRRDAIILGGTIFDLLEDYRGGGACELLIGRLRRDG
                     PVANLEVAFDLRIREVEEMWREYLRDLARPQPDSTSLPSPEELLGPIQPEP"
     gene            complement(1187..2338)
                     /locus_tag="PLE93_04795"
     CDS             complement(1187..2338)
                     /locus_tag="PLE93_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86401.1"
                     /translation="MLRRTVGVLAAFGLAVMMLFGAQSASAAGKTVTISGKAFIFNYM
                     DTPIPGATIKVREFPKLSAVTNDQGDYELTVPDDANVTPYIEPGGPVNLTRYNKDGSL
                     KGILDNTHWNTIDLQTFHTRGQDLENANFQTPRDAEYNGLKALLAIPSSPDGRPAQCA
                     IVTTSSARNVRGVDYLTYWNNTPHGVAGATSIEYPALDGPVYFNDSVIPDATKTESSG
                     DGGIIWPIVPTGTYRIVTTSPDTQFASFLATCKPGRVINANPPWGAYELSPGEKPLGA
                     SNVAAKLVSAKAVRKSKTRRQLSLVLNSGENIDATVTLAAGGMTALRTLPLKPGNKTL
                     TFNLGAKVKAKQAKVTVKLTDASGVSFTTVKKVNVPKTIKKKNGKGKRK"
     gene            complement(2419..>2657)
                     /locus_tag="PLE93_04800"
     CDS             complement(2419..>2657)
                     /locus_tag="PLE93_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86402.1"
                     /translation="TSIDGGIIWPVVETGTYTITTTSPTAGFASFTATCAPGRVVNAN
                     PPWGAFELVGAGEEPDGGPEFPAGCRVGAVPSAT"
BASE COUNT          409 a          861 c          887 g          500 t
ORIGIN      
        1 acaagcgcct gagctggaag ccgcgccatc cgctgcacct ggttctgctg cgggttcacc
       61 gcatcccgcg tccggtcacc gtccgggtcc gcgaggagta ccacggctgc cgctcctggg
      121 tcgagatcga ccgtgatctc cccttcgagg gcaccccggt catgtcggac gaggagttcg
      181 agttcgccac caacgagatc cgttcgatct gcgccggcga ccgcgaaccc gccttggtct
      241 aggcaacccc gcaatcgaga aggcaatagt ggccatatag cgctcccttt agccttctcg
      301 attttccaga ttcatgaaga aggcccgctc cggcgggtct ttttcattgc aataggctgg
      361 gccggtgacg tggatcgaga ccacatccct caccttcacc gcccggcacg aggaggggga
      421 ccgctcctac gccgaggagc tgctcgaccg gctggaggac ctgagcctca agctcgaaga
      481 ccgcttcgag gtggtgccgg gcgacatctc gatcgtcatc cacccttcgc cggtctggct
      541 gaccgccgcc catcccttcc tgcccttcgt ccgttatgcc gccgccccgg ccggccgccg
      601 ctacctggcc ggctggccga tggcgaccga ggtccacgtg ctgggcgaga aggcgatgga
      661 aaagcgggcg gccggcgagg cctcggccga ggccctgcgg ggcaccgccg agcggctcta
      721 cacccagatc gtgctggccg ccaacaacga gcggatcccg ccgccctgga ccccgatgcg
      781 cttcctccgc tacctgaact gggcctggct ggtcgagggc agcgcccagc atttcgcccg
      841 tcaggttgac ttcttccgcc ccgcggtgat ccgccggttg cgcgagggct cccgtcccag
      901 tttcccgcct tcccggcgtg acgcgatcat cctcgggggc acgatcttcg acctgctcga
      961 ggattaccgt ggcggcggcg cctgtgagct tttgatcggc cgcctgcgcc gcgacggccc
     1021 ggtcgccaac ctcgaggttg ccttcgacct ccggatcagg gaggtcgagg agatgtggcg
     1081 cgaatacctc agggacctgg ccaggcccca gccggattcg acctcgctgc cctcaccgga
     1141 ggagctgctc ggtccgatcc agccggagcc ctgaccgggg cgagcgctac ttgcgcttgc
     1201 ccttcccgtt cttcttcttg atggtcttcg gaacgttcac ctttttgacg gtggtgaacg
     1261 agacgccgga agcgtcggtc agcttgacgg tgaccttcgc ctgcttcgcc ttgacctttg
     1321 cgccgaggtt gaaggtcagc gtcttgttgc ccggcttcag gggcagcgtc cgcagtgcgg
     1381 tcatgccgcc ggccgcaagc gtcacggtgg cgtcgatgtt ctcaccggaa ttcaagacca
     1441 gggagagctg acgcctggtc ttgctcttgc ggacagcctt ggccgaaacg agcttggcgg
     1501 ccacgttgct ggcgccgagc ggcttctcgc cggggctgag ctcgtaggcg ccccacggcg
     1561 ggttggcgtt gatcacgcgg cccggcttgc aggtcgccag gaagctggcg aactgggtgt
     1621 cgggcgaggt ggtgacgatc cggtaggtgc cggtcgggac aatcggccag atgatgccgc
     1681 cgtctccgga agactcggtc ttggtcgcgt cggggatgac cgagtcgttg aagtagacag
     1741 ggccgtcgag ggccgggtac tcgatcgagg tggcaccggc cactccatgc ggagtgttgt
     1801 tccagtaggt caggtagtcg acgccccgca cgtttcgggc agacgaggtg gtgacgatcg
     1861 cgcactgggc cgggcggcca tcaggggaag aaggaatcgc aagcagcgcc ttgaggccgt
     1921 tgtattcggc gtcgcgaggc gtctggaagt tcgcgttctc gagatcctgg ccgcgggtgt
     1981 ggaaggtctg gaggtcaatc gtgttccagt gggtgttgtc gaggatgccc ttgagcgagc
     2041 cgtccttgtt gtagcgggtc agattcaccg ggccgccggg ctcgatgtac ggagtgacgt
     2101 tggcgtcgtc gggaaccgtc agttcgtagt caccctgatc gttggtgacc gcagaaagct
     2161 tgggaaactc gcggaccttg atcgtggccc ccgggatcgg cgtgtccatg tagttgaaaa
     2221 taaaagcctt gccgctgatc gtcacggtct tgcccgccgc cgacgccgac tgggcgccaa
     2281 acagcatcat cacggccagc ccgaaagcgg ccagcacgcc aacagttctc ctcaacatca
     2341 atctcctgct ttctcctgcc cgggtcgtcc cggacggtaa tgagtgtggt tccggaatct
     2401 acaacaggtt ggaccacatc aagttgcgga cgggaccgct cccacccggc acccagccgg
     2461 gaattcaggc ccgccgtccg gttcctcacc ggccccaacc agctcgaagg caccccaggg
     2521 cgggttcgcg ttgacgaccc ggccgggggc gcaggttgcc gtgaagctgg cgaagccggc
     2581 ggtcggggag gtggtggtga tcgtgtaggt gccggtttcg accaccggcc agatgattcc
     2641 gccgtcgatt gaggtct
//
LOCUS       DAOQFI010000222         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20355_length_2656_cov_2.891580, whole genome shotgun sequence.
ACCESSION   DAOQFI010000222 DAOQFI010000000
VERSION     DAOQFI010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20355_length_2656_cov_2.891580"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLE93_04805"
     CDS             complement(<1..216)
                     /locus_tag="PLE93_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86403.1"
                     /translation="MRTFLVASVSLIASAFALSGVAAAAEVPPCTGSFSQKTIYSGQG
                     SIEALIVGGGGTLFASGGDTTQSTLRAY"
     gene            276..1127
                     /locus_tag="PLE93_04810"
     CDS             276..1127
                     /locus_tag="PLE93_04810"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNH86404.1"
                     /translation="MANSIEDLKLVNSGKVREIYEIEDDLLLVASDRISIYDVIMPTA
                     IPDKGKVLTQMSNFWFENTTDIVPNHFLSNDVPEEVAGRAVRVRKLEMYPVECVVRGY
                     LSGSGWKEYQETGAVCGVGLPDGLQESDKLPRPIFTPATKAELGDHDENVDFDRAAEI
                     VGDRHLMEELRRISIEIYEYGANHAAEKGIILADTKFEFGSYAGAEIVLGDEVLTPDS
                     SRYWPADQYEPGKSQPSFDKQYVRDWANAAGWDHNPPAPELPEDVVAQTQAKYREAYE
                     RITGREL"
     gene            1124..1354
                     /gene="purS"
                     /locus_tag="PLE93_04815"
     CDS             1124..1354
                     /gene="purS"
                     /locus_tag="PLE93_04815"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNH86405.1"
                     /translation="MIQARVLIRPKEGILDAQGKAVEGALPALGFSGIEHVRIGRIVE
                     LEADSTDQLEELCEKLLANPLIEDYEIETVSG"
     gene            1351..2058
                     /gene="purQ"
                     /locus_tag="PLE93_04820"
     CDS             1351..2058
                     /gene="purQ"
                     /locus_tag="PLE93_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNH86406.1"
                     /translation="MKWGVLQFPGSCDERDALNACQIAATALGSEGGSGSLPRLVWHE
                     ETDLSDVDGIVVPGGFSYGDYLRAGAIARFSPAMTAVAEFAAAGGPVLGICNGFQVLC
                     ESGLLPGALLTNEKTRFICRQGELIVGEAQSPFLSGYEAGQRISIPFKHQSGRFFAPA
                     DLLEEIESNNQVAFRYAPGQNPNGSLNDIAGVTNEAGNVIGLMPHPEHAVEAFAGSTD
                     GLALFQGPAIAATGAVA"
     gene            2055..>2656
                     /locus_tag="PLE93_04825"
     CDS             2055..>2656
                     /locus_tag="PLE93_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNH86407.1"
                     /translation="MSEQSAQREDKGEQSAHREDKGDQSAHRALGLTDREFDLICEKM
                     GREPNGVELAMFSLLWSEHCAYKHSRKLLKKLPTEGPRVVMGPGENAGAVDIGNGFSV
                     AFKVESHNHPSAVEPFQGAATGVGGILRDVIALGARPIAILDSLRFGEPDTERTHYLV
                     DGVVRGIGHYGNSMGIPTVGGEIYFEPPYEQNPLVNAMCVG"
BASE COUNT          495 a          855 c          859 g          447 t
ORIGIN      
        1 gtaggcccgc agggtgcttt gggtcgtgtc accgccggaa gcgaaaaggg ttccgccacc
       61 gccgacgatc aacgcctcga tcgaaccctg cccggaatag atggtcttct ggctgaaaga
      121 gccggtacag ggaggcactt cagccgccgc cgcgactccc gaaagggcga aagccgacgc
      181 gatcaggctc acggaagcaa ccagaaaagt gcgcatttgc ggaggttata cgcagtccgg
      241 ctttccggct tgtcgattgg gtagtttgtc ctttaatggc gaattcgatt gaagacctca
      301 agcttgtcaa ttccggaaag gtccgcgaga tctacgagat cgaggatgac ctcctcctcg
      361 tcgcctccga ccgcatctcc atctacgacg tgatcatgcc gaccgcgata cccgacaagg
      421 gcaaggttct gacccagatg tccaacttct ggttcgagaa caccacggac atcgtgccga
      481 accacttcct ctcgaatgac gtgccggagg aagtcgccgg gcgggcggtc cgggtgcgca
      541 agctcgagat gtacccggtc gagtgcgtgg ttcgcggcta cctctccggt tccggctgga
      601 aggagtacca ggagaccggc gcggtctgcg gggtcgggct gcccgacggc ctgcaggaat
      661 ccgacaagtt gccccggccg atcttcaccc cggcgaccaa ggccgaactc ggcgaccacg
      721 acgagaacgt ggacttcgat cgggccgccg agatcgtcgg tgaccgccac ctgatggagg
      781 aactgcgccg gatctcgatc gagatctacg aatacggcgc aaaccacgcg gccgaaaagg
      841 ggatcatcct cgccgatacc aagttcgagt tcggcagcta cgccggggcc gagatcgtgc
      901 tcggtgacga ggttctgact cccgactctt cgcgctactg gccggccgac cagtacgagc
      961 ccggcaagtc gcagccttcc tttgacaagc agtacgtccg cgactgggcc aacgccgccg
     1021 gctgggatca caatccgccg gccccggagt tgccggaaga cgtggtcgcc cagacccagg
     1081 ccaagtaccg cgaggcctac gagcggatca ccggaaggga actttgattc aggcccgcgt
     1141 tctgatccgg cccaaggagg gcatcctcga tgcccagggc aaggcggtcg aaggggcgct
     1201 gccggcgctt ggtttctccg ggatcgaaca cgtcaggatc ggccggatcg tcgaactcga
     1261 agcggacagc accgatcagc tcgaggagct ttgcgagaag ctcctcgcca acccgctgat
     1321 cgaggattac gagatcgaga ccgtcagcgg atgaagtggg gtgtgcttca gttccccggg
     1381 tcctgcgacg agcgggacgc gctcaacgcc tgccagatcg ctgcgacagc cttgggctct
     1441 gagggcgggt ccggctcgtt gcctcggttg gtttggcatg aggagacgga tctgtctgac
     1501 gtcgacggga ttgtggtgcc tggtggtttc tcttacgggg actaccttcg ggcgggggca
     1561 attgcccggt tttctccggc gatgaccgcg gtggccgagt tcgccgcagc cggcgggccg
     1621 gtcctgggga tctgcaacgg attccaggtt ctctgcgagt cggggcttct gcctggcgcg
     1681 ctgctgacca acgagaagac ccggttcatc tgccgccagg gcgagctcat cgtgggcgag
     1741 gcccagtcac ccttcctctc cggatacgaa gccggccaga ggatctcgat ccccttcaag
     1801 caccagtccg gccgcttctt cgcgccggcc gacctgctcg aggaaatcga gtcgaacaac
     1861 caggtcgcct tccggtacgc cccgggccag aatccaaacg gctcgctgaa cgacatcgcc
     1921 ggcgtgacca acgaggccgg caacgtgatc ggcctgatgc cgcaccccga acatgcggtc
     1981 gaggccttcg ccggctcgac cgacggtctc gcgctcttcc agggcccggc gatcgccgcc
     2041 accggagctg tcgcgtgagc gagcaaagcg cgcagcgcga agacaaaggc gagcagagcg
     2101 cgcatcgcga agacaaaggc gatcagagcg cgcatcgcgc tctcggcctg accgatcgcg
     2161 agttcgatct gatctgcgag aagatgggcc gcgagccgaa cggggtcgaa ctggcgatgt
     2221 tctcgctgct ctggtccgag cactgcgcct acaagcactc gcgcaagctt ctgaagaaac
     2281 tgccgaccga aggtccccgc gtggtcatgg gcccgggcga aaacgccggc gcggtggaca
     2341 tcggcaacgg cttctcggtc gccttcaagg tcgaatccca caatcaccct tcggcggtcg
     2401 aaccgttcca gggcgcggcg accggtgtcg gcggcatcct ccgcgacgtg atcgcactcg
     2461 gcgcccgtcc gatcgcgatc ctcgattcgc ttcgcttcgg cgagccggac accgaacgga
     2521 cccattacct ggtcgacggc gtggtccgcg ggatcggcca ctacggcaat tcgatgggca
     2581 tcccgacggt cggcggcgag atctacttcg agccacccta cgaacagaac ccgctggtca
     2641 acgcgatgtg cgtcgg
//
LOCUS       DAOQFI010000223         2652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20409_length_2652_cov_2.644590, whole genome shotgun sequence.
ACCESSION   DAOQFI010000223 DAOQFI010000000
VERSION     DAOQFI010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20409_length_2652_cov_2.644590"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="PLE93_04830"
     CDS             <1..1410
                     /locus_tag="PLE93_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNH86408.1"
                     /translation="RFEAESPRIEAKGSARRKIGPADYQAERVIYLAPAARFGALLDL
                     PEGSDLGKAVNEAMRLVEEHNPDLAGVLPRNYSSIPNETIVSLLRHISSYTKSVEGDA
                     FGLIYEYFLGRFALAEGQGAGEFFTPMSIVKLIVEIIEPFHGRILDPACGSGGMFVQS
                     AYFVERHQGSPGNELSVFGQEKTGETVKLAKMNLAIHGLSGDVREGNSYYEDPHESHG
                     RFDFVMANPPFNVDKIDKAKLEDDPRFPLLPKADNGNYIWIQLFANSLNEQGRAGFVM
                     ANSASDARHSEAEIRQRLIEDRLVDVMVAVSPNFFYTVTLPVTLWFLDRGKKGGERED
                     QVLFIDARQVFNQIDRAHRDFTTEQLEFLANIVRLYRGEDPEFAEDKNELFDERFPEG
                     EFADVTGLCKVATIEEIKAQGWSLNPGRYVGTEVEELEDEVFEEKLAEAQAELRVLGA
                     QARELEEGVDAVLTQLLEK"
     gene            1487..2644
                     /locus_tag="PLE93_04835"
     CDS             1487..2644
                     /locus_tag="PLE93_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNH86409.1"
                     /translation="MEIERSQPTRFGEARRTPGAHGYVAYFPAPLVRSIELPSSTVNL
                     LADTEASLGELSAVGRLLPNPDLLIRPYMLREAVSSTRIEGTQAEMSEVFDVDAARSD
                     PNPDVEEVVNYVSAMNWGLAQLDRLPLSSRLILEIHRRLMTGVRGRNLSPGEFRTTQN
                     WIGPPGSTIETATFVPPPPTELGDLLSDWEKFANEKPEMPLLVQNALLHVQFESIHPF
                     LDGNGRLGRLLLIFFLVARKRLGTPILYLSSYLEQHRSSYYEALQLAHESGDPFPWID
                     LFLTAVRTQSADGVIRSQKIIELRDRYRQAAGELGTSNAMDVVDLICENPVINARKVE
                     ESLDVSRPTALRLLQRMEEKGVLTEEEKGARGQRRYVAQEMMNAVTGGSGE"
BASE COUNT          558 a          768 c          794 g          532 t
ORIGIN      
        1 cgctttgaag ccgagagtcc gaggatcgaa gccaagggct ccgcccggcg gaagatcggc
       61 ccggctgatt accaggctga acgggtcatc tacctcgctc cggcggctcg ttttggagca
      121 ttgctcgatc tcccggaagg ttccgacctt ggtaaggcag tcaatgaagc gatgcgactg
      181 gtcgaggagc ataaccccga tctggccgga gttctgccgc gcaactactc ctcgattcca
      241 aatgaaacga tcgtctcgct ccttcgacac ataagcagct atacgaagag cgtcgagggt
      301 gatgccttcg gactgatcta cgagtacttc ctcggcaggt tcgccttggc ggagggtcag
      361 ggtgccggtg agttctttac gccgatgtcg atcgtgaaac tgatcgtcga gatcattgaa
      421 cccttccacg ggcggatcct agacccggcc tgcgggtccg gcggaatgtt cgttcagtcg
      481 gcctatttcg tcgagcgtca ccaggggtct ccgggcaacg aactctccgt cttcggccag
      541 gagaagaccg gcgagacggt caagctcgcg aaaatgaacc tcgcgatcca cgggctctcc
      601 ggggacgttc gtgagggcaa cagctattac gaggacccgc acgaaagcca cgggcgcttc
      661 gatttcgtga tggccaaccc accgttcaac gtcgacaaga tcgacaaggc gaagctggaa
      721 gatgacccgc gctttccgct gctgccgaaa gctgacaacg gcaactacat ctggatccag
      781 ctatttgcca attcgctcaa cgagcagggt cgggccggat tcgtgatggc taattctgcc
      841 tccgatgccc gtcacagcga ggcagagatt cgccagcgac tgatcgagga ccgactggtc
      901 gacgtgatgg tggctgtcag cccgaacttc ttctacaccg tcacccttcc ggtaactctc
      961 tggtttctcg accggggcaa gaagggcggc gaacgggaag accaggttct cttcatcgat
     1021 gctcgccagg tcttcaacca gatcgatcgt gcccaccgtg atttcactac cgagcaactg
     1081 gagtttctgg ccaacatcgt tcgcctttac cggggcgaag acccggaatt tgcagaggat
     1141 aagaacgagc tcttcgatga gcgtttcccg gaaggtgaat ttgcagacgt gaccggactc
     1201 tgcaaggtcg cgacgatcga ggagatcaag gcccagggct ggagcctcaa cccgggccga
     1261 tacgtgggga ccgaggtcga agaacttgag gatgaagtgt tcgaggagaa gctggccgag
     1321 gcccaggctg agttgagggt tctcggagcc caggcgagag agctggaaga aggggtagat
     1381 gcagtgctga cccaacttct cgagaagtag cctttcaccg acgttagggt cgatctctga
     1441 ccattacgtt tcacatgaga ttcgttagag aggctaggat tcgttgatgg agatcgaacg
     1501 ttcccagccg acgcgtttcg gggaggcccg gcgcaccccg ggtgctcacg gctacgtggc
     1561 gtatttccct gcgccgctgg tcaggtcgat cgagctgccg tcttcgaccg tcaatcttct
     1621 ggctgatacc gaagcgtctt tgggagaact atccgcggtc ggccgcctcc tgccgaaccc
     1681 tgaccttctc atccgtccct acatgttgcg ggaagcagtc tcctcgaccc ggatcgaagg
     1741 gacccaggct gaaatgtcag aagtatttga tgtcgatgcc gcccgttccg accctaaccc
     1801 tgatgtcgag gaggtcgtca attacgtctc ggccatgaac tggggtctgg cccagcttga
     1861 ccgcctgccg ctcagcagcc gattgattct ggagatccac cggcgcttga tgaccggggt
     1921 ccggggccga aacctttcgc ccggtgaatt ccgcaccacc cagaactgga tcggcccacc
     1981 cggttcgaca atcgaaacag ccactttcgt acccccaccg ccgaccgagc tgggcgacct
     2041 gctgagtgat tgggagaagt tcgcaaacga gaagccagag atgccgcttc tggtccagaa
     2101 cgccctgcta cacgttcagt tcgagtcgat ccatccgttc cttgacggca atggccggct
     2161 cggccggctt ctcttgatct tcttcctggt cgcgaggaaa aggctgggta cgccgatcct
     2221 ttacttgagt tcctacctgg agcagcaccg gtcgagctac tacgaagccc ttcaattggc
     2281 gcatgaatca ggcgatccgt ttccttggat tgatcttttc ctgaccgcgg tgcgaaccca
     2341 gtccgctgat ggagtcatcc ggagccagaa gatcattgag ctccgggacc ggtaccgtca
     2401 ggcggcaggg gagcttggaa cctcgaacgc catggatgtc gtcgacctga tttgtgagaa
     2461 cccggtgatc aacgcaagaa aagtcgagga aagcctcgat gtcagccgac ccaccgctct
     2521 ccgactcctg caaagaatgg aagaaaaagg agtcctcacc gaggaagaaa aaggagcccg
     2581 cggccaacgc cgctatgtgg cccaagaaat gatgaacgcg gtgaccgggg gttccggtga
     2641 gtgagtggcg gg
//
LOCUS       DAOQFI010000224         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20457_length_2649_cov_3.825752, whole genome shotgun sequence.
ACCESSION   DAOQFI010000224 DAOQFI010000000
VERSION     DAOQFI010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20457_length_2649_cov_3.825752"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLE93_04840"
     CDS             <1..544
                     /locus_tag="PLE93_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HNH86410.1"
                     /translation="AAPAAGATAGEGYLYCGPAGAGHFVKMVHNGIEYGMMAAIAEGL
                     NVIKGANAGVITRDGDAETAPMENPEYYQYEIDVSQVAEVWRRGSVVGSWLLDLTAAS
                     LAESPTLEEFSGRVSDSGEGRWTSIAAIDEGVPTPVLTAALHERFYSRGLGDFGDKVL
                     SAMRKQFGGHDEKPAEGKEG"
     gene            545..1219
                     /gene="pgl"
                     /locus_tag="PLE93_04845"
     CDS             545..1219
                     /gene="pgl"
                     /locus_tag="PLE93_04845"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNH86411.1"
                     /translation="MVTRFVEDDESAAAEAARIVELAAEAAIADRGRFNFAVSGGKTP
                     WRMLELLAESDLNWTRTSLFQVDERIAPTGSMQRNLTHLVLTLPLICQAAIRPMPVGA
                     DDLATAASGYEYSLPDRFDLIHLGMGTDGHTASLVPGDPILEVSDRQVAITSGEYQGT
                     RRMSLTYKALDRARQILFLVTGDDKEEALAKLVDSDPSIPAGRLKNPNVTLITDIDIR
                     DSEPVS"
     gene            1216..1884
                     /locus_tag="PLE93_04850"
     CDS             1216..1884
                     /locus_tag="PLE93_04850"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNH86412.1"
                     /translation="MTKIREMGAERYGFALLLILASMIFIMATHDGKWSNVISLNLQA
                     FALFASTRAAQPRMAIRVTVGVIIGLVLVAAWIQAGFTSSVNDDFVRLSTFSLVVIAT
                     PAIAWGLVRQVKARGMITTHTMLGVLCIYLLMSLAFSAAFGAISAVSGDPFFQQGAAD
                     GTTNNYLYYSLTTITTVGMGDFTPATNLGRSLTAAEALIGQIYVITVVAVIVGNLGRP
                     RNNG"
     gene            1877..>2649
                     /locus_tag="PLE93_04855"
     CDS             1877..>2649
                     /locus_tag="PLE93_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNH86413.1"
                     /translation="MDDVLLGLSLVVVLSILARLSATWMRVPPIVPLLIVGMAIGISG
                     LDLVNPSEMLGPALSPAVQIAVGIILFEGALSLKREELGSGVRSAVTRLITLGVVITW
                     FVATVSIALLLDVPHEMSILIGATVIVSGPTVVIPILNFVGPTQKVRRVLKWEGILID
                     PIGAIIAVVVFGSFDNGTGGEFDIGELFLSLGVGGLIGAAGAAVMIPILGTKRLAGRD
                     KVAATLMMVVAAFAASDAVLSDSGLVATIVMGVVLANQHR"
BASE COUNT          422 a          875 c          862 g          490 t
ORIGIN      
        1 ggccgcgccc gcggcggggg ccactgcggg cgagggttac ctctactgcg gcccggccgg
       61 cgctggccac ttcgtgaaga tggtccacaa cgggatcgag tacgggatga tggccgcgat
      121 cgccgagggg ctgaacgtga tcaagggcgc caacgccggc gtgatcaccc gtgacggaga
      181 cgccgagacc gcaccgatgg agaaccccga gtactaccag tacgaaatcg acgtctccca
      241 ggtcgccgaa gtgtggcgcc ggggcagcgt ggtcggttcc tggctgctcg acctcaccgc
      301 cgcgtcgctg gccgagtccc cgaccctgga ggagttctcc gggcgggttt cggattccgg
      361 cgagggtcgc tggacctcga tcgccgcgat cgacgaagga gtgccgaccc cggtcctcac
      421 cgccgctctg catgagcggt tctactcgcg cggcctgggc gacttcggtg acaaggtgct
      481 ctcggccatg cgcaagcagt tcggcggtca cgacgagaag cccgcggagg ggaaggaagg
      541 gtaattggtc acccggttcg tcgaggacga cgagtcagcc gcggccgagg ccgcccggat
      601 tgtcgagttg gcggccgagg ccgcgattgc cgaccggggg cgcttcaatt tcgcggtcag
      661 tggcggcaag acgccctggc ggatgcttga actgctggcc gaaagcgacc tcaactggac
      721 ccggacctcg ctgttccagg ttgacgagcg gattgcgccg accggcagca tgcagcgcaa
      781 cctcacccac ctggttctca ccctgccgct gatctgccag gccgcgatcc ggccgatgcc
      841 ggtcggggcc gatgacctcg cgacggcggc cagcggttac gagtattcgc tgccggaccg
      901 cttcgacctg atccacctcg gcatgggcac ggacgggcac accgcctcgc tggtgccggg
      961 ggacccgatc ctcgaggttt ccgaccgcca ggttgcgatc acttccggcg agtatcaggg
     1021 cacccgtcgc atgtcgctga cctacaaggc gcttgatcgc gcccggcaga ttctcttcct
     1081 ggtcaccggg gacgacaagg aggaagcgct cgcgaagctg gtcgactccg acccgtcgat
     1141 tccggccggg cggctcaaga accccaacgt caccctgatc accgacatcg acatcaggga
     1201 ttcggaaccg gtttcttgac gaagatccgc gaaatggggg ccgagcgcta cggcttcgcg
     1261 ctacttctga tcctcgcctc gatgatcttc atcatggcca ctcatgacgg gaaatggtcc
     1321 aacgtcattt ccctgaacct tcaggccttt gcgctgttcg cttcaacccg ggcggcccag
     1381 ccgaggatgg cgattcgggt cacggtcggt gtgatcatcg gactggtcct cgtcgccgca
     1441 tggattcagg ccgggttcac cagttcggtc aacgatgatt tcgtgcggct ttcgaccttc
     1501 agcctcgtcg tgatcgcaac ccccgcgatc gcctgggggc tggtgcggca ggtcaaggct
     1561 cggggaatga tcaccaccca cacgatgctc ggcgtgctct gcatctacct gctgatgagc
     1621 ctcgcgttct cggctgcgtt cggggcgatc tccgcggtca gcggcgaccc gttcttccag
     1681 cagggcgccg ccgacggcac gaccaacaac tacctctact acagcctgac cacgatcacc
     1741 acagttggca tgggcgactt caccccggcg accaacctgg gccgttccct caccgcagcc
     1801 gaggcattga tcgggcagat ctacgtgatc acggtggtcg ccgtgatcgt cggcaacctg
     1861 ggcagacccc gaaacaatgg atgacgtcct acttggcctg agcctggtcg tcgtcctctc
     1921 gatcctcgcc cggctctcgg ccacctggat gcgggtgccg ccgatcgtcc cgctgctgat
     1981 cgtcggcatg gcgatcggca tctccggtct cgatctcgtc aacccgagcg agatgctggg
     2041 cccggcgctc tcgcccgcgg ttcagattgc ggttggcatc atccttttcg aaggggcgct
     2101 cagcctgaag agggaggagc tcggttccgg ggtgagaagc gcggtgaccc gactgatcac
     2161 gctcggggtc gtaatcacct ggtttgtcgc cacggtctcg atcgccttgc tgctcgacgt
     2221 tccccacgag atgtcgatcc tgatcggcgc gacggtaatc gtttccgggc cgaccgtggt
     2281 gatcccgatc ctcaatttcg tcggtcccac ccagaaggtt cgccgggtcc tgaaatggga
     2341 gggcatcctg atcgacccga tcggggcgat catcgcggtg gtcgtcttcg gatccttcga
     2401 caacggaacc ggcggcgagt ttgacatcgg cgagctcttt ctttcgctcg gggtcggcgg
     2461 cctgatcggg gcggccggcg ccgcggtcat gatcccgatc ctcggaacca agcggctggc
     2521 cggacgggac aaggtcgcag ccaccctgat gatggtggtc gccgcctttg ccgcctcgga
     2581 cgccgtgctc tccgattcgg gcctggtcgc gacgatcgtc atgggagtcg ttctcgccaa
     2641 tcagcatcg
//
LOCUS       DAOQFI010000225         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20568_length_2643_cov_2.852782, whole genome shotgun sequence.
ACCESSION   DAOQFI010000225 DAOQFI010000000
VERSION     DAOQFI010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20568_length_2643_cov_2.852782"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..537)
                     /locus_tag="PLE93_04860"
     CDS             complement(31..537)
                     /locus_tag="PLE93_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86414.1"
                     /translation="MEEKSARRGFLLSKGWLQAVIIVMLLGFTVLGILAYRTYEDGAP
                     VPVKVLDPQGQLLFDGDDITAGQQVFLHNGLMEYGSVFGHGAYLGPDYTADYLRRSSN
                     IAIAENGGPPVDEDGMELADDPDPGPASDIARQKTINQFRENQFDEESNELTFSQTQT
                     DAFNKLIR"
     gene            691..2001
                     /locus_tag="PLE93_04865"
     CDS             691..2001
                     /locus_tag="PLE93_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNH86415.1"
                     /translation="MTATATARRVPPMVDESVLSQIWKVSTAKNEIFDQSRARYGDAF
                     GMNINRGKFYFLAHPDQVKEVFTASPEVLHAGKGNEILATALGEHSVLLLDGSEHMRQ
                     RKLLLPSFHGEKLEAQTGAMERISEEVVASIPRGESFHMRETSQKIALEVILEIVLGT
                     ASTGEQHDRLGKATRELLEWIGKGYRLALSSVFGPRSRPIKRMYKPALAPLDQGLYEL
                     IAERRQATGLEDRHDILSMLLLARDEDGQPMTDLEIRDELVTLIVAGHETTATALAWA
                     FERLTRVPGGTERLHEEAVAGEGEYARWVSQEALRLRPVLDFVLRRVMEPIEIGGYQF
                     GEGDVLAPSIYLVHRREDVYPDPLAFKPERWDGVKPGTYTWFPFGGGVRRCIGMQFAM
                     AEMEIVMKAVARAGILLPVGPQEPAVARFITSSPKRGGQVRFAF"
     gene            complement(2076..>2643)
                     /locus_tag="PLE93_04870"
     CDS             complement(2076..>2643)
                     /locus_tag="PLE93_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNH86416.1"
                     /translation="VLFSHPKDFTPVCTTELGYMANAKPAFDERGVKIIGLSVDPLDS
                     HEDWAKDIEETQGTAPNYPLISDADFKIAKAYGMLAADVEGDAADRTPADNQTVRNVF
                     VIGPDKKIKLILVYPMTTGRNFDEVLRVIDSLQLTANHKVATPANWKSGDDVIIAGSV
                     NNDQAKEIFGEWEEPKPYIRIVPDPTAS"
BASE COUNT          447 a          836 c          868 g          492 t
ORIGIN      
        1 agcgccgcac actgcgagag gtgaccagcg tcaccggatc agtttgttga aggcgtcggt
       61 ctgggtctgc gagaaagtga gttcgttgga ctcctcgtcg aactggttct cgcggaactg
      121 gttgatcgtc ttctgccggg cgatgtccga ggccggcccg gggtcgggat cgtcggcgag
      181 ctccatcccg tcctcgtcga ccggtggccc gccgttctcg gcgattgcga tgttggacga
      241 gcgacgcaga tagtccgcgg tgtagtcggg cccgaggtag gcgccgtggc cgaagaccga
      301 gccgtactcc atgagtccgt tgtgcaggaa gacctgctgg ccggccgtga tgtcgtcacc
      361 gtcgaaaagc agttgcccct gggggtcaag gaccttgacc ggtaccggtg cgccgtcttc
      421 ataggtgcga taggccagga tgccgagcac ggtgaagccg agcagcatga cgatgatgac
      481 cgcctgcagc cagcctttgc tgagcaggaa ccctcgacgg gcagacttct cctccacgga
      541 tctccttccg cagttcgccc ttgcgggcga gtatggactg ggacgagtgc ttctggcagg
      601 ctttgtaaca cctcaaccgg caaatgtcca tgtggacttc tacggaactt gatgtgaaac
      661 gaagcgttgt caatcatcta gtgtgaagcc atgactgcca ccgctaccgc gcgccgcgtc
      721 ccgcccatgg tcgacgagtc agtgctctcc cagatctgga aagtctcgac cgccaaaaac
      781 gaaatcttcg accagtcacg ggccaggtac ggcgatgcct tcgggatgaa catcaaccgc
      841 gggaagttct atttcctggc ccaccccgat caggtcaagg aggtcttcac cgcctccccg
      901 gaagtgctcc acgccggcaa gggcaacgag atccttgcga cggcgctggg cgagcattcg
      961 gtactgctgc tcgacggctc cgaacacatg cggcaacgca agctgctcct cccctccttc
     1021 cacggcgaga agctggaagc acaaaccggc gcgatggagc ggatctcgga ggaggtggtg
     1081 gcttcaatcc ccaggggcga gagcttccac atgcgggaga ccagccagaa gatcgcgctc
     1141 gaggtcatcc tcgagatcgt gctcggcacc gcctcgaccg gtgagcagca tgaccgcctc
     1201 ggcaaggcca cgcgcgagtt gctcgaatgg atcggcaagg gttaccgcct cgccctttct
     1261 tccgtgtttg gcccgcgcag ccggccgatc aaacggatgt acaagccggc cctggcgccg
     1321 ctcgatcagg gcctctacga gctgatcgcc gagcgtcgcc aggcgaccgg cctggaggat
     1381 cgccatgaca tcctctcgat gctgctgctg gcccgcgacg aagacggtca gccgatgacc
     1441 gacctggaga tccgcgacga actcgtcacc ctgatcgtgg ccggccacga gaccacggcg
     1501 accgcgctgg cctgggcctt cgaacggctg acccgggtgc ccggcggcac cgagcggctc
     1561 cacgaggaag ccgtcgccgg cgagggcgaa tatgcccgct gggtttccca ggaggcgctg
     1621 cggctccggc cggtcctcga cttcgtcctc cgccgggtga tggaaccgat cgagatcggc
     1681 ggctaccagt tcggggaagg cgacgtactc gctccctcga tctacctcgt ccatcgccgc
     1741 gaggacgtct atcccgaccc gttggcgttc aagccggagc gctgggacgg ggtcaagccc
     1801 ggcacctaca cctggttccc cttcgggggc ggtgttcgcc gctgcatcgg catgcagttc
     1861 gcgatggccg agatggagat cgtgatgaag gcggtggccc gggccgggat ccttttgccg
     1921 gtcggccccc aggagcctgc cgtggcccgc ttcatcacct cctcgccgaa gcgcggcggc
     1981 caggtccgct tcgccttcta gcggaagcgg gacaaagctt cgagaaggcg gaagtcagcc
     2041 ctattgactg acttccgcca tctcggtaaa tccgatcagc tggcggtcgg gtccgggacg
     2101 atccggatgt acggcttggg ctcttcccac tcgccgaaaa tctccttcgc ctggtcattg
     2161 ttgaccgagc cggcgatgat cacgtcgtcg ccgctcttcc agttggccgg ggtggcgacc
     2221 ttgtggttgg cggtcagctg cagcgagtcg atcacgcgga ggacctcgtc gaagttccgg
     2281 ccggtcgtca tcgggtagac caggatcagc ttgatcttct tgtccggacc gatcacgaag
     2341 acgttgcgga cggtctggtt gtcggccggg gtgcgatcgg cggcgtcacc ctcgacgtcg
     2401 gcggccagca ttccgtaggc cttggcgatc ttgaagtcgg cgtcggagat cagcgggtag
     2461 ttcggggccg tgccctgggt ctcctcgatg tccttggccc agtcctcgtg gctgtcgagc
     2521 ggatcgaccg agagcccgat gatcttcacg ccgcgctcgt cgaaggccgg cttggcattc
     2581 gccatgtaac cgagttcggt ggtgcagacg ggggtgaagt ccttcggatg cgaaaagagg
     2641 act
//
LOCUS       DAOQFI010000226         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20741_length_2629_cov_3.756022, whole genome shotgun sequence.
ACCESSION   DAOQFI010000226 DAOQFI010000000
VERSION     DAOQFI010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20741_length_2629_cov_3.756022"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..758
                     /locus_tag="PLE93_04875"
     CDS             99..758
                     /locus_tag="PLE93_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86417.1"
                     /translation="MIELLLPGAVPDFVREAQSILREGSTFQWSTVTLLGLVVYVYSV
                     EVERRRWDIVLAGLAFSMMDLFNEMVNSAIFHLTDRAPLWTVTGDTSYLIFIGLTIEI
                     LLLFAVSGVAFVKTLPPDRKAKILGIPNRVVFVTVFSLFCVAVEIFLHSTGYFHWEYW
                     WWNVPFVPLIVIFGYATFFAVAAWVYDMGSNTRKQVRVVGTMAAINLVLGLALGLAGW
                     L"
     gene            983..1774
                     /locus_tag="PLE93_04880"
     CDS             983..1774
                     /locus_tag="PLE93_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNH86418.1"
                     /translation="MKICVLVKDVPDAAVQKRIDPGTMRLDRSGENTLNPFDTHAIEA
                     AMQLREGGELQVDEIVAVTMGTDTAARALQKAVALGADRSVHLSDDALAGSDVCATGL
                     ALAKVLESEGPDLVLLGQQSDDGECYTIGAVVADHLKMPSLTQVIDMKVDGDKIRCER
                     QAEYGYDTVALTLPAVISVGDAINEPRYPSLKAIMGAKKKPLDSKSAADVGIDDGQVG
                     EAGSQTTVISLNEPPQKEAGEIIEDEDTAETVEKIVAWLEERKLI"
     gene            1775..>2629
                     /locus_tag="PLE93_04885"
     CDS             1775..>2629
                     /locus_tag="PLE93_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNH86419.1"
                     /translation="MAGILVYALHDDEGNFNKNSLGALSEAAKLAGELGTEAAAVIVG
                     DISDDGAAGLGNYGVTKVYRAKDVPEGLAQPIVDVMAKVIQDNGYTYALFGGGLLGFE
                     IGAGLTARLGAGVTMEVTKVKVDGGKLVSERPILQDSKIADVGYVSEPGIIIGRLNAF
                     DVNESGGTAEVVDVDVELSEFSTKAEMITRGEQRGADVDIEAADILIGGGRGLGSADG
                     FKDCEELAGAFSNGAVAATRAVVDAGWYPYAAQIGQTGKTVAPKLYLAVGVSGAIQHK
                     VGMQNSENI"
BASE COUNT          475 a          870 c          822 g          462 t
ORIGIN      
        1 aggtacaggg tcgaggccag ttcgatgggt ctcggcggcc ggctcgccac ccgcgcggtg
       61 ttcgtgcggc gaaagcaggg tcatttctcg gtgatcggat gatcgagttg ctgcttcccg
      121 gggcggtccc cgacttcgtc cgggaggccc agtcaatcct gcgcgaagga tccaccttcc
      181 agtggtccac ggtcaccctg ctcggcctgg tcgtctacgt gtattcggtc gaggtcgaac
      241 gccggcgctg ggacatcgtg ctggccgggc tcgccttctc gatgatggat ctcttcaacg
      301 agatggtcaa ctcggcgatc ttccacctga ccgaccgggc gccgctctgg accgtgaccg
      361 gtgacaccag ctacctgatc ttcatcggcc tgacgatcga gatcctgctg ctcttcgcgg
      421 tttccggggt tgccttcgtc aagaccctgc cgcccgaccg gaaggcgaag atcctcggga
      481 tcccgaaccg ggtcgttttc gtgaccgtct tctcgctctt ctgcgtggcg gtcgagatct
      541 tcctgcacag caccggctac ttccactggg agtactggtg gtggaacgtg cccttcgtcc
      601 cgctgatcgt gatcttcggc tacgccacct tcttcgcggt cgccgcctgg gtctatgaca
      661 tgggatcgaa cacccggaaa caggtcaggg tggtgggcac catggccgcc ataaatctgg
      721 ttctaggcct ggcattggga ctggccggtt ggttgtaggg actccctgat cccctcccga
      781 caaacattga cttcgagtgg gaggggcggg ttccaggccg gagggcgggt gaagacccgc
      841 ccgactgcag aagccctctg attgactcgt cagtcaactt ttggcgaatc ccgatagaat
      901 cccgcgtcgc aaaacagcat ctccggctct ccgcccttga attcggcaga gcccctcgta
      961 agacccccta aggaggcttc agttgaagat ttgcgtcctg gtcaaagatg tgccagacgc
     1021 cgcggtacaa aagcgcattg atcccggcac catgcggctc gatcgcagcg gcgagaacac
     1081 tctcaacccc tttgacaccc acgccatcga ggctgcgatg cagcttcgcg aaggcggcga
     1141 actccaggta gacgaaatcg tcgccgtgac gatgggcacc gataccgctg cccgcgccct
     1201 gcagaaggcc gtcgccctcg gcgccgaccg ttcggtccac ctgtccgacg acgccctcgc
     1261 cggctccgat gtctgcgcca ccggcctcgc cctggccaag gtcctcgagt ccgagggccc
     1321 ggacctggtc ctgctcggcc agcagtcaga tgacggcgag tgctacacga tcggtgccgt
     1381 tgtggccgat cacctgaaga tgccgtcgct gacccaggtc atcgacatga aggtcgacgg
     1441 cgacaagatc cgctgcgagc gtcaggccga gtacggctac gacaccgtgg ccctgaccct
     1501 gccggccgtg atctcggttg gtgacgcgat caacgagccg cgttacccga gcctcaaggc
     1561 catcatgggc gccaagaaga agccgctgga cagcaagtcc gccgctgacg tcggcatcga
     1621 cgacggccag gtcggcgaag ccggctcaca gacgaccgtc atttcgctga acgagccgcc
     1681 ccagaaggaa gccggcgaga tcatcgaaga cgaagacacc gcggaaacgg tcgagaagat
     1741 cgttgcgtgg ctcgaggaaa ggaagctgat ctaaatggcc ggtattctcg tttacgccct
     1801 tcacgatgac gagggcaact tcaacaagaa ctcgctcggc gccctctccg aggccgccaa
     1861 gctggccggc gagctcggca ccgaagccgc agccgtaatc gtcggcgaca tttccgacga
     1921 cggagctgcc ggtctcggca actacggcgt gaccaaggtc taccgcgcga aggacgttcc
     1981 ggagggcctc gcccagccga tcgtcgacgt gatggccaag gtcatccagg acaacggtta
     2041 cacctacgcc ctgttcggcg gcggcctgct cggcttcgag atcggtgccg gcctgaccgc
     2101 ccgcctcggc gccggcgtga ccatggaagt caccaaggtc aaggttgacg gtggcaagct
     2161 cgtctccgag cgtccgatcc tgcaggactc gaagatcgcc gacgtcggct acgtctccga
     2221 gcccggcatc atcatcggcc gcctcaacgc cttcgacgtg aacgaaagcg gcggaaccgc
     2281 cgaggtcgtc gacgtcgacg tcgagctctc cgagttctcg accaaggccg agatgatcac
     2341 ccgcggcgag cagcgtggcg ccgacgtgga catcgaagcc gccgacatcc tgatcggtgg
     2401 cggtcgtggc ctcggctcgg ccgacggctt caaggactgc gaggaactcg ccggtgcctt
     2461 cagcaacggt gcggttgccg caacccgcgc ggtggtcgac gccggctggt acccctacgc
     2521 cgcccagatc ggccagaccg gcaagacggt cgcgccgaag ctgtacctgg cggttggcgt
     2581 ctccggcgcc atccagcaca aggtcggcat gcagaactcc gaaaacatc
//
LOCUS       DAOQFI010000227         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20745_length_2629_cov_3.144134, whole genome shotgun sequence.
ACCESSION   DAOQFI010000227 DAOQFI010000000
VERSION     DAOQFI010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20745_length_2629_cov_3.144134"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1961
                     /locus_tag="PLE93_04890"
     CDS             210..1961
                     /locus_tag="PLE93_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86420.1"
                     /translation="MPSPRNFIPVLAALATLLLAPASFAAKDPAFVPSRNSPIQTAGT
                     PIGVAPYDQASSESGLLVLNRRPAGIQVFENRQETPLLPAHRFRVKEDPLSLQTFTDT
                     WSEVDAVGVVRHDGSFSFYRVTHSSSKGYGLVSGNVGIDIGPVTALAKYHGLGIDSAP
                     FGRVKVVAVDTADDSLILVSQVPGFPDAIEATVPVGDQPVDIYSENAIAMFVVNRGSD
                     DISVVRYFQDPDETGEIGQGNYGHWEVTDTAPVGESPVAIAAIPSRQGRNLFAVANSG
                     SDDVSILRLNPDSSMDELGRVPVGDRPVALTSVQMTANGRWRLAVANAGSDDVTIIEG
                     EFNGTFAATDTIPVGSNPVSITSGDFDEYFDADLAVADRASGQVSVLLDNEPSGTCRG
                     GAAKLLRGGQYSDSLFGTHNPDQILGLTGDDEIHGGKDGFDCLFGGKGKDSLDGYKLG
                     DLLDGGPDADTLTGGTGTDLVIGGAGDDFLCENLEEEMKQEACGVPYRDYSSWGRVGR
                     PERDRLFGGPGNDRIQADYGRDLVSGGSGRDDIFSRDGARDLVTCGAGRDRALVDDRD
                     RVRGCETVKRIEVSRES"
     gene            complement(1945..>2629)
                     /locus_tag="PLE93_04895"
     CDS             complement(1945..>2629)
                     /locus_tag="PLE93_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNH86421.1"
                     /translation="FGDQFEDTIRNTTYPSLVEALNAVHAKAPKARVAILGYPWIMPE
                     TDGCYPQMPVSKGDVPYVRSLQRTLNDAVGRAARETGSVFVNLNGVSNGHDACQAPGT
                     RWVEPALLGENPVIVHPNALGEAKMAEQAFKVLSKSGTPAPAAPRTRISKVLISNGKR
                     RATFRLESTGSAPTFRCRLDGQGFRRCRSPKTYRGLKTGSHTFRVVAIDGNGLADRTP
                     AVFRFRIRG"
BASE COUNT          438 a          890 c          863 g          438 t
ORIGIN      
        1 gaggcatttg ttgatggggc acggcaaccc gatcaacggc aagcaggcca cagtcgccct
       61 ccagcaggcg ctcgaccgct cccgccggga cctccccggc accatgctca agatcccctt
      121 cgccttccgc tgagggctcc agagcgaaac tcccccattg cgcggggttt ctttcggtga
      181 gtcgattcgt ccagtctgta aggaagcaga tgccaagtcc ccgcaacttc atcccggtcc
      241 tcgccgccct cgcgaccctg ctgctcgccc cggcctcctt cgcggccaaa gatcccgcct
      301 tcgtgccttc ccgcaactcg ccgatccaga cggcgggaac gcccatcggg gtggctccgt
      361 atgaccaggc cagcagcgag tcggggctgc tggtcctgaa tcgccggccg gccggcatcc
      421 aggtcttcga gaaccggcag gaaacgccgc tgctgccggc ccacaggttc agggtcaagg
      481 aggatcctct ttccctccag accttcaccg atacctggag cgaggttgac gcggtcggcg
      541 tagtgcgcca tgacggcagc ttctcgttct atcgcgtgac ccactccagc agcaagggtt
      601 acggcctggt cagcggcaat gtcggcatcg acatcggtcc cgtcacggcc ctggcgaagt
      661 accacgggct tggcatcgac tcggcccctt tcggccgggt gaaggtagtg gccgtcgaca
      721 ccgccgatga ctccctgatt ctggtcagcc aggttcccgg cttccccgac gcgatcgaag
      781 cgacggtgcc ggttggcgat cagccggtcg acatctactc cgagaatgcg atcgccatgt
      841 tcgtggtgaa ccggggctct gacgacatca gcgtcgtccg gtacttccag gatcccgatg
      901 agaccgggga gatcgggcag ggaaactacg gccattggga agtcaccgac acggccccgg
      961 tcggtgagag cccggttgcg atcgcggcga tcccttcccg gcagggccgg aacctcttcg
     1021 ccgtcgccaa ttccggatcc gatgatgtct cgatcctgcg cctgaaccct gacagcagca
     1081 tggatgagct gggcagggtc ccggtcggcg accggccggt cgccctcacc tccgtccaga
     1141 tgacggcgaa cggtcgctgg cgcctggccg tcgccaacgc cggctcggat gacgtgacca
     1201 tcatcgaggg tgaattcaac ggaacctttg ccgccacgga cacgatccca gtcggctcca
     1261 atccggtctc gatcacctcg ggtgacttcg atgaatactt cgacgccgac ctcgccgtgg
     1321 ccgaccgggc cagtggtcag gtctcggtcc tgcttgacaa cgagccctcc ggcacctgcc
     1381 gcggcggtgc cgcgaagctg cttcgcggcg gccagtactc ggattcgctg ttcggcaccc
     1441 acaacccgga ccagatcctc gggctgaccg gagatgacga gattcacgga ggcaaggacg
     1501 gcttcgactg cctcttcggc ggcaagggca aggacagcct ggacggctac aagctcggcg
     1561 acctcctcga cggcggcccg gacgccgaca ccttgaccgg cggaaccggg accgaccttg
     1621 tgatcggcgg ggccggcgac gatttcctct gcgagaacct ggaagaggag atgaaacagg
     1681 aagcctgtgg cgtcccctac cgcgactact cgagctgggg ccgcgtcggc cgacccgagc
     1741 gggaccggct gttcgggggc ccgggcaatg accgcatcca ggctgactac ggacgcgacc
     1801 tggtcagcgg gggctccggc cgggacgaca tcttcagccg cgacggcgcg cgggatctcg
     1861 tcacctgcgg cgcgggccgg gatcgggcgc tggttgacga ccgggaccgc gtgcgcggtt
     1921 gcgaaacggt caaacggatc gaagtcagcc gcgaatcctg aacctgaaga cggccggggt
     1981 cctgtccgca aggccgttgc cgtcgatcgc cacgacccgg aaagtgtggc tgccggtctt
     2041 caggccacgg taggtcttcg gtgaccggca tctccggaaa ccctgaccgt caaggcggca
     2101 ccggaaggtc ggagcggagc cggtcgactc aagccggaag gtggcccgcc gcttgccgtt
     2161 gctgatcagc accttgctga tccgcgtgcg gggagcggcc ggcgccggcg tgcccgactt
     2221 cgaaagcacc ttgaaggcct gctcggccat cttcgcttca ccgagggcgt tcgggtggac
     2281 gatcaccggg ttctcgccaa gcagcgccgg ctcgacccag cgggttcccg gggcctggca
     2341 ggcgtcgtgc ccgtttgaca cgccgttcag gttgacgaag accgaaccgg tttcacgggc
     2401 cgcgcgtccc accgcatcgt tcagcgtccg ctgcagcgaa cggacatagg gaacgtcacc
     2461 cttcgagacc ggcatctgcg ggtagcagcc atcggtctcg ggcatgatcc aggggtagcc
     2521 gaggattgcg acccgggcct tcggcgcctt cgcgtgaaca gcgttcaggg cttcgaccag
     2581 tgacgggtag gtggtgttcc ggatcgtgtc ctcgaactgg tcgccgaag
//
LOCUS       DAOQFI010000228         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20803_length_2625_cov_3.168482, whole genome shotgun sequence.
ACCESSION   DAOQFI010000228 DAOQFI010000000
VERSION     DAOQFI010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20803_length_2625_cov_3.168482"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLE93_04900"
     CDS             complement(<1..364)
                     /locus_tag="PLE93_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HNH86422.1"
                     /translation="MRALAEGEQLLTLRKGGIREENKHFELEHDRFFLYPTFDHQHND
                     LVRASHHPELDRALEEGVWPDEEPPARALLQDGGIPQPDRVRIRAWAEVAEDITIEDP
                     RVVDALSPFYIWTTDYAHK"
     gene            complement(577..2454)
                     /locus_tag="PLE93_04905"
     CDS             complement(577..2454)
                     /locus_tag="PLE93_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNH86423.1"
                     /translation="MPESPAQAILGVDVGGTFTDAVLITAEGLFTGKSPSTPEDQSVG
                     VIEATRQVLARAGIEPEQVGHFAHGMTVATNALLESKGAETAFVATRGFTDLIEIGRQ
                     DRASLYRLQAAPPPPLVPPERRVGVAERNGPEGALEELTEAEAGRVAEAVAGLDVESV
                     AVCLLHSYRFPENEQRIGEAIRQRCGEDLHVSLSHDVVGTFREFERAATTEIDAALSP
                     LLAGYLERLARRSDEEGLPRPEIMQSSGGVAGLEEVADHATLAILSGPAGGAAAASLI
                     AEQSGEKNLLCFDMGGTSCDVCVVEGGRVHEAASKKIAGRPIALPMVDIDTVGAGGGS
                     IAWRDSGGALRVGPESAGARPGPACYGHGGTRPTVTDANLVLGRLNAGEELAGGIVLD
                     LDLARQAIETLAGQLGIPTDRCAEGIIRVANAEMVRALRVVTVQRGLDPRRFTLLAFG
                     GAGPLHAAGVAEELGISTVLVPPTGGVFSALGLAAADRRRDEVQTVLAAEDSLDRQRL
                     LDLIADADETGWDLRYRGQSFELTVSDDSADPARLRELFEAAHEERYGYRDPASPIEL
                     VTVRRRWVVPGPGFAPAPPSETVLSGPATIDLGEATLHLPVGWNAEGEGGGLLRMFRE
                     L"
     gene            complement(2447..>2625)
                     /locus_tag="PLE93_04910"
     CDS             complement(2447..>2625)
                     /locus_tag="PLE93_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNH86424.1"
                     /translation="GGAPGERGRNLLVRKDGSAEDLEPKSAGRLEPGDRLRIETPGGG
                     GFGTGGGAGSSLDA"
BASE COUNT          416 a          871 c          911 g          427 t
ORIGIN      
        1 gcttgtgggc gtagtcggtg gtccagatgt agaacgggga cagcgcgtcg acgacccgcg
       61 ggtcctcgat cgtgatgtct tcggctactt ccgcccaggc acggatgcgg actcggtcgg
      121 gctgagggat cccgccgtcc tggagaagcg cccgggccgg cggctcctcg tcgggccaga
      181 cgccctcctc gagggcacgg tccagctccg ggtgatggga ggcgcgtacc aggtcgttgt
      241 gctggtgatc gaatgtgggg tagaggaaga agcggtcgtg ctcgagctcg aagtgcttgt
      301 tctcttcgcg gattccgccc ttgcggagcg tgagaagctg ttcaccttcg gcgagggctc
      361 gcacggtaac tgcccattcc ttgaacgcaa ttggcatagg tattcagtcg ggggtagtgg
      421 ttgctacgga taagtggtac ggcagacacc tgtacggggc gagaggaggc atgactggct
      481 gccgcgggcc ccgtaaggag ttggcactct acaggtcgat ccggcgaaat tcaagtcctc
      541 gtgccaaaaa ttacgtacta acccgtattc acgtactcaa agttcacgaa acatgcggag
      601 caggcctccg ccttcgccct cggcattcca gccgaccggc aggtgcagag tcgcttcacc
      661 aaggtcgatc gttgccggtc cggagaggac cgtttcggag ggcggggccg gagcgaaacc
      721 gggaccgggc acgacccagc gccggcggac cgtgaccagc tcgatcggcg atgccgggtc
      781 ccggtacccg tagcgctcct cgtgtgccgc ctcgaagagt tcgcggaggc gggccggatc
      841 ggccgagtcg tcggagacgg tgagttcgaa ggactgtccc cggtagcgga gatcccagcc
      901 ggtctcgtcg gcgtcagcga tcagatcgag caggcgctgg cggtcgagcg aatcctcggc
      961 ggcgagcacg gtctgaactt catcccggcg acgatcggcg gcggccaggc cgagggcgct
     1021 gaagacgccc ccggttggag gcaccaggac agttgaaatg cccagctctt cggcgacccc
     1081 ggccgcatga agcggcccgg cgccaccgaa ggccagcagg gtgaaccgcc ggggatcgag
     1141 cccgcgctga acggtcacga cgcgaagggc gcgaaccatt tccgcgttgg cgacccggat
     1201 aattccctcg gcgcaccggt cggtcgggat cccgagctga ccggccaggg tttcgatcgc
     1261 ctgccgggcc aggtcaaggt cgaggacgat gccgccggcc agctcttcac cggcgttcag
     1321 ccggccgaga acgagatttg cgtcggtcac ggtcggccgg gtgccgccgt gcccgtaaca
     1381 ggccggtccc ggacgggctc cggcggattc ggggccgacc cggagtgctc cgccgctgtc
     1441 gcgccaggcg atcgaaccgc caccggcgcc gaccgtgtcg atatcgacca tgggcagggc
     1501 gattggccgg ccggcgatct tcttcgaggc tgcctcgtgg accctgcctc cctcgaccac
     1561 gcagacgtca caggaggtgc cacccatgtc gaaacagagc aggttcttct cgccggactg
     1621 ttcggcaatc agtgacgcgg cggccgcccc gccggccggt cccgaaagga tcgccagcgt
     1681 cgcgtggtca gcgacctcct ccagcccggc gacgccgccg cttgactgca tgatctccgg
     1741 tctcggcagg ccctcttcat cggagcggcg ggcgaggcgt tcgagatagc ccgccagcag
     1801 gggggagagg gcggcgtcga tttcagtggt cgcagcccgt tcgaattccc ggaaggtgcc
     1861 gaccacgtca tgcgagagcg agacatggag atcctccccg cagcgttgcc ggatcgcctc
     1921 gccgatccgc tgctcgttct ccgggaagcg gtacgagtga agcaggcaga ccgcgaccga
     1981 ttcgacgtcc agcccggcaa ccgcttcggc cacccggcct gcttccgctt cggtcagctc
     2041 ctcgagcgct ccctcgggcc cgttccgctc ggccaccccg acccggcgct cgggcgggac
     2101 cagtggcggc gggggggcgg cctggagccg gtagagcgaa gcgcggtcct ggcggccgat
     2161 ctcgatcagg tcggtgaaac cgcgggtcgc aacgaaggca gtctcggctc ccttcgattc
     2221 gagcagggcg ttggtcgcca cggtcattcc gtgggcaaaa tggccgacct gttccggctc
     2281 gatgcccgcc ctggcgagga cctgcctggt cgcttcgatc accccgaccg actggtcttc
     2341 gggggtggaa ggtgacttgc cggtgaagag cccttcggcg gtgatcagca cggcatcggt
     2401 gaaggtgccg ccgacatcga ccccgaggat cgcctgtgcc gggctctcag gcatcaaggc
     2461 tggaccccgc gccgccaccg gttccgaaac cgcccccgcc cggagtctcg attctcaggc
     2521 ggtctcccgg ttcgagccga ccggctgact tcggttcgag atcctcggcc gagccatctt
     2581 tccggaccag caggttccgg ccccgctcac cgggagcacc acctt
//
LOCUS       DAOQFI010000229         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_20976_length_2612_cov_3.610090, whole genome shotgun sequence.
ACCESSION   DAOQFI010000229 DAOQFI010000000
VERSION     DAOQFI010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20976_length_2612_cov_3.610090"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLE93_04915"
     CDS             <1..1035
                     /locus_tag="PLE93_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH86425.1"
                     /translation="KRIIEFLPKVRPSWFFAVPRIWEKLKAGLESNLASLPEEQREKA
                     QAGLQAAIQKVRLEQAGEEVPEELAAAVAAADEALFSHLRVALGFDEALAVSAGAAPT
                     PAEVLEFFHAIGIPVGELWGLSETCGVITINPPKKIKIGTVGPPVPGVEMRVAEDGEL
                     LCRSPFVMKEYRNKPEKTAETIVDGWLLTGDIGEIDEEGYVTILDRKKELIINAAGKN
                     MSPANIEAQIKVSSPLINQAVTIGDSRPYNVALIVLDPDFAPVWASQNGIEGSFEDLA
                     GDERIEAAIQAAVEEANGKLARVEQIKKFKLIPGEWLPGGDELTPTMKLKRKPIAEKY
                     ESEIEDLYST"
     gene            1243..2193
                     /locus_tag="PLE93_04920"
     CDS             1243..2193
                     /locus_tag="PLE93_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86426.1"
                     /translation="MEDETAPATTLQAGIVVLLGLLRAEEAETDQPFSTGALRTRILG
                     DLSGPDVTAGELGLALWAESRCGGEAIGEIDGLIRRATVGGLEKVPTEQIAWLASGLA
                     ETAVLTGDPSTGLIREVKAELSARITPGGLARDVHARRLGVAVPVTGQFHLLHALVQL
                     VRAGEEDCRNRADQLAGALLALQREDGAWPGLIDPVRSEAAVVYPVLAVTQIAAAPIA
                     LRAATELGLGDFEAAAKSGIQWANGGNALGLSLVHEADARIDRGILTKRKPGALERGI
                     SSAARRIRGQAFEPDAKQLIIDPAASSEDLGWVLEAWAGR"
     gene            2344..>2612
                     /locus_tag="PLE93_04925"
     CDS             2344..>2612
                     /locus_tag="PLE93_04925"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNH86427.1"
                     /translation="MTKTETANLEATMEQINTVAEAGADIVRVAVPRDKDIEALKTIV
                     KQSPIPIIADIHFNHTLALKAIDAGADCIRLNPGNIGGRDKVAEVA"
BASE COUNT          509 a          821 c          880 g          402 t
ORIGIN      
        1 aagcggatca tcgagttcct gcccaaggtg cgaccctcct ggttctttgc agtcccccgg
       61 atctgggaga agctgaaagc cggactggaa tcgaacctgg cctcgctgcc ggaagaacag
      121 cgcgaaaagg cccaggccgg acttcaggcg gcgatccaga aggtacggct ggaacaggcc
      181 ggggaagagg tgccggaaga gctggccgcc gcggtcgcag cggccgacga ggcgctcttc
      241 tcgcacctgc gggtcgccct aggcttcgac gaggcgctgg ccgtaagtgc cggagctgca
      301 ccgaccccgg ccgaggtgct cgagttcttc catgcgatcg gcatcccggt cggcgagctc
      361 tggggcctct ccgagacctg cggcgtgatc acgatcaacc cgccgaaaaa gatcaagatc
      421 ggcacggtcg gaccaccagt tcccggggtc gagatgcggg tggccgagga tggcgagctg
      481 ctctgccgca gccccttcgt gatgaaggag taccgcaaca agcccgagaa gacggccgag
      541 acgatcgtcg atggctggct tttgaccggt gacatcggcg agatcgacga agagggttac
      601 gtgacgatcc tcgaccgcaa gaaggagctg atcatcaacg cggccggcaa gaacatgtca
      661 ccggccaaca tcgaggcgca gatcaaggtc tcttcacccc tgatcaacca ggcggtgacg
      721 atcggagaca gccgccccta caacgtcgcg ctgatcgtgc tcgaccccga cttcgctccg
      781 gtctgggcct cccagaacgg gatcgagggt tccttcgagg acctggccgg tgacgagcgg
      841 atcgaagcgg ccatccaggc cgcggtcgag gaagccaacg gcaaactggc ccgggtcgag
      901 cagatcaaga agttcaagct gatcccgggt gaatggttgc cgggtggcga tgaactgacg
      961 ccgaccatga agttgaagcg gaaaccgatc gccgagaagt acgaatccga gatcgaggat
     1021 ctctactcca cctgagagtc tccgggaccg gtaccgatag gccccttcct cggcccgaaa
     1081 cgtcgtcagg gacctatcgg caccggtcca gatgcttcga gcgcatataa cctcggctta
     1141 tagatgatcg aaccgcgcgc cctggagcgc atcgcactta caaggttgcc tgagctgcag
     1201 gttgcttccg ggctttttcg ggctactgcg cggattgagg gggtggagga tgagacggct
     1261 ccggcgacga cgcttcaggc cgggatcgtg gtgctcctcg ggctgttgcg ggccgaagag
     1321 gccgagaccg accaaccctt ttccaccggc gcgctgagga cccgaatcct aggtgacctg
     1381 agcggtccgg acgtgaccgc cggtgaactc ggactcgcgc tctgggccga atcgaggtgc
     1441 ggcggagaag cgatcgggga aatcgatggc ttgatccggc gggcaacggt cggaggcctg
     1501 gagaaggttc cgaccgagca gatcgcctgg cttgcctccg gtctggccga gaccgcggtg
     1561 ctgaccggcg acccgtcgac cggactgatc agggaagtca aggccgaact gagcgcccgg
     1621 atcacgcccg gcggcctcgc cagggacgtc cacgcccgaa ggctgggagt cgcagtcccg
     1681 gtcaccggcc agtttcacct gctccacgcc ctggttcagc tggtcagggc gggcgaggaa
     1741 gactgccgga acagggccga tcagctggct ggggcgttgc ttgccctcca gcgcgaggat
     1801 ggcgcatggc ccggtttgat cgatcccgtc cgttctgaag ctgccgtcgt gtatccggtc
     1861 ctggcagtca cccagatcgc ggccgcaccg atcgcgctcc gggctgccac ggaactcggg
     1921 ctgggcgact ttgaagcggc ggcaaaatcc ggcattcagt gggccaacgg aggcaacgca
     1981 ctcgggctct ccctggtcca tgaagcggat gcgcggatcg accggggcat cctgaccaaa
     2041 cgaaagccgg gcgctctgga acgcggaatc agttccgctg cgaggcgaat ccggggtcag
     2101 gcattcgagc ccgacgccaa acaactgatc atcgatccgg ccgcctcgag cgaggacctc
     2161 gggtgggtcc tcgaagcctg ggccggcagg tagccaccac aaaaggccgc tggcgccccg
     2221 ccgcgggcga tcgccttgtc tgcggccccc gatcggtagc ctgcaggggt agtggcatcg
     2281 aaaagacaga tattcgtcgg ccgggtaccg gtgggcggcg gagcgccggt cgcggtccag
     2341 acaatgacca agaccgagac cgccaacctc gaagcgacga tggagcagat caacacggtc
     2401 gccgaagccg gcgccgacat cgttcgggtc gcggttcccc gtgacaagga catcgaggcg
     2461 ctgaagacga tcgtcaagca gtcgccgatc ccgatcatcg ccgacattca cttcaaccac
     2521 accctggccc tgaaggcaat tgacgctggt gccgactgca tccgcctgaa tccaggcaac
     2581 atcggcgggc gcgacaaggt cgccgaggtg gc
//
LOCUS       DAOQFI010000230         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21002_length_2611_cov_2.196401, whole genome shotgun sequence.
ACCESSION   DAOQFI010000230 DAOQFI010000000
VERSION     DAOQFI010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21002_length_2611_cov_2.196401"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PLE93_04930"
     CDS             <1..821
                     /locus_tag="PLE93_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH86428.1"
                     /translation="RLRAAFFVSGDRRGIWRRAGYCRAGRARRPGAGAGLMLPVREGI
                     ARIVVCEDDQPTLELLCEHLTADRFSVLAAPSAADAMRLCLYNDPDLMLLDLGLPDAA
                     GLDLLREIRNPDRTVNRVDPGIGVIIVSGRSTERDRVRGLSEGADDYVQKPFSYGELL
                     ARINSVLRRRRRSHSLVQVGEIEINDVEHTVRVGGRYLRLSMKEYALLKILANEPSRV
                     FSKEELLQEVWGYRSSGHTRTLEAHCSRLRKKLDPKHGRYVVNCWGVGYRLVGP"
     gene            833..1573
                     /locus_tag="PLE93_04935"
     CDS             833..1573
                     /locus_tag="PLE93_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH86429.1"
                     /translation="MSIGLADLIPLLAALFVLAGREAWLAHRRVLVNRALHEARRPLQ
                     AIALSLPAGPGRMTPVLPVWQAIQALGEVDRQINGGAPDRPRPEPIACRLMTEACVRR
                     WQSRARMAGGGIEMRWTGPEALVTGDPAALSAAIENLIVNAIEHGGPRIVVRALVIGK
                     RLRLEVTDNGKAHRAAGRSGSPAEVIARLRGKDRHGHGLPVVREAAREHAGRFEINLD
                     ESGSTATLTLPCATRAPAPAPGLVGTEA"
     gene            1580..2221
                     /locus_tag="PLE93_04940"
     CDS             1580..2221
                     /locus_tag="PLE93_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86430.1"
                     /translation="MGRRRRATLMLLAAAGAGILAVSLVGGYSSSVAESYGELRPVVV
                     LTRGLAPGQVISPKVAAASFEIRQVPARFVPAEALGDPTRAVGLEPVGPMPAGSYLTG
                     PGLMVPGSDRPKRRPLGHGRHAVEISVAGAGALSGGGKVDVLVTTDNDRGGGRTLVAA
                     RRVPLISIGRTGQSEAGPGLTEVTLGLTRGEAIRLVDAQTFARRITVLPPAGP"
     gene            2223..>2611
                     /locus_tag="PLE93_04945"
     CDS             2223..>2611
                     /locus_tag="PLE93_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNH86431.1"
                     /translation="MDDRGRLAAEIRAGLIERRRAGAGTDQEGIEQAIRHAVEARAVM
                     LSEAAQEDLVERILREASGLGPLEVLLDDGSVEEVMVNGPDLVYVERGGKIEPAGVRF
                     EDEEQLRNTIERILAPLGRRVDELSPMA"
BASE COUNT          392 a          835 c          991 g          393 t
ORIGIN      
        1 gccgcctccg ggcggccttc ttcgtttcag gggaccggcg cgggatctgg cggagagccg
       61 ggtactgccg agccggacgg gcccggcgac ccggggcggg ggcggggctg atgcttccgg
      121 tgcgggaagg gatcgctcgg atcgtcgtct gcgaggacga ccagccgacc ctcgaactgc
      181 tctgcgaaca cctgaccgcc gaccgtttct cggtcctggc tgcgccgagc gccgccgatg
      241 cgatgcggct ctgcctctac aacgatccgg acctgatgct gctcgacctt ggtttgcccg
      301 acgcggccgg gctcgacctg ctgcgcgaga tccgcaatcc ggatcggacc gtgaaccggg
      361 tcgatccggg aatcggggtg atcatcgtca gcggtcgcag caccgagcgc gaccgggtcc
      421 gcggcctcag cgaaggggcg gacgattacg tccagaagcc cttcagctac ggcgagctgc
      481 tggcccggat caattcggtg ctgcggcgcc ggcggcgcag ccattccctc gttcaggtcg
      541 gggaaatcga gatcaacgac gtcgagcaca cggtccgggt gggtggccgc tacctgcgac
      601 tttcgatgaa ggagtacgcg ctgctgaaga tcctcgccaa cgaaccgagc cgggtcttct
      661 cgaaggagga actgctgcag gaggtctggg gataccgctc ctccgggcac acccggaccc
      721 tcgaagcgca ctgcagccgg ctgcggaaga agctcgatcc gaagcacggc cgttacgtgg
      781 tcaactgctg gggagtcggc taccggctcg tgggtccgtg aggggtgctg aagtgagtat
      841 tggacttgcc gacctgatcc ccctgctggc cgcgctcttt gtacttgccg ggcgggaagc
      901 ctggctggcc catcgccgcg tcctcgtcaa tcgggcgctc catgaagccc ggcggccgct
      961 ccaggcgatc gcgctctccc tgccggcggg gccgggccgg atgaccccgg tgctgccggt
     1021 ctggcaggca atccaggcgc ttggcgaggt cgatcggcag atcaacggcg gtgcacccga
     1081 ccggccgagg cccgagccga tcgcctgccg gttgatgacc gaggcctgtg tgcggcgctg
     1141 gcagtcacgg gcccggatgg ccggaggcgg gatcgaaatg cgctggaccg gaccggaggc
     1201 attggtgacc ggggatccgg ccgcgctctc ggcggcgatc gagaacctga tcgtcaacgc
     1261 gatcgagcac ggtgggccgc ggatcgtggt gcgtgccctg gtgatcggca agcggctgcg
     1321 gctggaggtc accgacaacg gcaaggccca ccgtgccgcg ggacgctccg gcagcccggc
     1381 cgaagtgatc gcgaggctgc gtggcaagga ccggcacggc cacgggttgc cggtggtccg
     1441 ggaggccgcc cgtgaacatg ccggccgctt cgagatcaac ctggatgagt ccggttcaac
     1501 cgcgaccctg accctgccct gcgcgacccg ggccccggct ccggcaccgg ggctggtcgg
     1561 gaccgaggcc tgaaccggga tgggccggcg ccggcgggca acgctgatgc ttctggccgc
     1621 cgccggtgcc ggaatcctcg cggtgagcct ggtcggcggg tactcctcct cggtcgccga
     1681 gagctacggc gagctgaggc cggtcgtggt cctgacccgc ggtctcgctc ccgggcaggt
     1741 gatctcaccg aaagtggcgg cggctagttt cgagatccgg caggtgccgg cacgtttcgt
     1801 accggcggaa gcgctgggtg atccgacccg ggctgttggc ctggaaccgg tcgggccgat
     1861 gccggccggc agctacctga ccgggcccgg gctgatggtg ccgggcagcg accggccgaa
     1921 acgcaggccg ctcggtcacg gccggcatgc ggtcgagata tcggtggcag gagccggggc
     1981 cctgagcggg ggcggaaagg ttgacgttct ggtcacgacc gataacgacc ggggtggagg
     2041 ccggaccctg gtcgcggcgc gacgggtccc tttgatctcg atcggccgga ccggtcagag
     2101 cgaggctggc ccgggactga ccgaggtgac cctggggctg acccggggcg aagcgattcg
     2161 cctggttgac gcgcagacct tcgcccggcg aattaccgtg ctgccaccgg cggggccctg
     2221 agatggatga ccgggggagg ctggcggccg agatccgggc cggtctgatt gagcggcgcc
     2281 gggccggtgc cggcacagat caggaaggaa tcgagcaggc gatccggcat gcggtcgaag
     2341 cccgggcggt gatgctctcc gaagcggcgc aggaggatct ggtcgagcgg atcctccgtg
     2401 aggcatccgg cctggggccg ttggaggttc tccttgatga cggcagcgtc gaggaagtca
     2461 tggtcaacgg gccggacctc gtctacgttg aacggggcgg aaagatcgaa ccggccgggg
     2521 tcaggttcga agacgaggaa cagcttcgga acacgatcga gcggatcctc gccccgctcg
     2581 gcaggcgggt cgacgagctc agcccgatgg c
//
LOCUS       DAOQFI010000231         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21059_length_2606_cov_3.267738, whole genome shotgun sequence.
ACCESSION   DAOQFI010000231 DAOQFI010000000
VERSION     DAOQFI010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21059_length_2606_cov_3.267738"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..666
                     /locus_tag="PLE93_04950"
     CDS             52..666
                     /locus_tag="PLE93_04950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNH86432.1"
                     /translation="MEEIAFFIGAIGALGAAIAVIAVRNPFYSVLALVIHLVFLAGIF
                     LLLMAQFLAAALIVVYAGAVMVLYVFVAAYVGGIEEPKWDSSPELRIVGPILAVALFI
                     AVSIAILGSGLSALGTPGPDDVPSGFGTPEAVGHLMMEDFLIAFEAASLLLLVAAVAA
                     IVLAGRRREDRPSDLSKRKSMVARDENVNAAIAGDPAAGRSEEA"
     gene            666..965
                     /gene="nuoK"
                     /locus_tag="PLE93_04955"
     CDS             666..965
                     /gene="nuoK"
                     /locus_tag="PLE93_04955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNH86433.1"
                     /translation="MDIEWYLILSALLFTVGFIGVLVRRNPVVILLCLELMLSAGALA
                     LLAFSRMLGNQDGQVFVLIVLVVAACEVVVGLGLVVAMYRRRLPLDVDEMKELKG"
     gene            962..>2606
                     /gene="nuoL"
                     /locus_tag="PLE93_04960"
     CDS             962..>2606
                     /gene="nuoL"
                     /locus_tag="PLE93_04960"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNH86434.1"
                     /translation="MTATWAWLVLLLPLLGSMTIGLTFRVLPARTAGLIGVTAIVAAF
                     VCGILALIGLQGEPSESRHVASSLWEYASVGGLSIDLGIYVDPLSIFMVLVVTGVSSL
                     IHIYSYSYMKSDQGYHRFFSYLNFFVFSMLVLVLAGNFIMLIVGWALVGFASYALISY
                     WYRRNTATKAGMKAFVINVIGDIGLYLAAILIFKNIGSVDYGTVFEKAPEVFTTNEWE
                     IVAICLCLLVGAFAKSAQMPFHTWLADAMEGPTPVSSLIHAATMVTAGVYLIARCHSL
                     FELAPTAADVSAWVGLVTLLIAGTIAIAVTDLKRIIAYSTMSQIGYMFVGVSIGAYSA
                     GMFHLMTHAFFKALLFMAAGSFISAMANMQDIDRMKGFRKAMPFTAAMMIIGALALSG
                     FPGTSGWFSKDEIIDFAHFRGGMYDWMWVGMLVGAFMTAVYSFRIIFRILPGPPCEEA
                     EYLEEHGHVIHGEPKNPATGELEDTEVGYPGEEHHIAEQAGGMKFAMAVLGVLALIAG
                     VLQIPGLDDVVTKFLEPTFENSVFSHMHPTTSESWIGLGE"
BASE COUNT          377 a          866 c          827 g          536 t
ORIGIN      
        1 gctgctggcc gagccgatcg aacggactcc gctgcgggga gagggcgagt agtggaagag
       61 atcgccttct tcatcggcgc gatcggcgcc ctgggtgcgg caatcgcggt gatcgcggtc
      121 cggaacccgt tctacagcgt gctggccctg gtcatccacc tggtcttcct ggccgggatc
      181 ttcctgctct tgatggccca gttcctggcc gccgccctga tcgtcgtcta cgccggtgcg
      241 gtgatggtgc tctacgtctt cgtcgccgcc tacgtcggcg gcatagagga gcccaaatgg
      301 gactcctcgc cggagctcag aatcgtcggt ccgatccttg cggtggccct gttcatcgcg
      361 gtttcgatcg ccattctcgg ctccgggctt tccgcgctcg gcacccccgg cccggatgac
      421 gtgccttccg gcttcggcac gccggaagcg gtcggccacc tgatgatgga ggacttcctg
      481 atcgccttcg aagcggcctc gctgcttctg ctggtcgctg cggtcgcggc aatcgtgctg
      541 gccggccgcc gccgtgagga ccgtccttcc gatctttcga agcgcaagtc catggtcgcc
      601 cgggacgaga acgtgaacgc cgcgatcgcc ggggatccgg ccgccggcag gagcgaggag
      661 gcctgatgga catcgagtgg tacctgatcc tttcggccct gctcttcacg gtcgggttca
      721 tcggggtgct ggtgcgccgc aacccggtgg tgatcctgct ctgcctcgag ctcatgctga
      781 gcgccggggc cctggccctg ctcgctttca gccgcatgct gggcaaccag gatggccagg
      841 tcttcgtgct gatcgtgctc gtggtggcgg cctgtgaggt cgtggtcggg ctcgggctgg
      901 tcgtcgcgat gtatcgccga cggctgccgc tcgacgtcga cgagatgaag gagctcaagg
      961 gatgaccgcc acctgggcct ggctcgtcct gctgctgccc ctgctcggtt cgatgaccat
     1021 cggactgacc ttcagggtgc tgccggcccg caccgccggg ctgatcggcg tcaccgcgat
     1081 cgtcgccgcc ttcgtctgcg gcatcctcgc cctgatcggt ctgcagggtg aaccttcgga
     1141 gtcccggcac gtcgcctcga gcctctggga gtacgccagc gtgggcggac tttcgatcga
     1201 cctcgggatc tatgtcgacc cgctttcgat cttcatggtg ctggtcgtca ccggcgtttc
     1261 cagcctgatt cacatctact cgtacagcta catgaagtcg gatcagggct accaccgctt
     1321 cttcagctac ctgaacttct tcgtcttttc gatgctggtc ctggtcctgg ccggcaactt
     1381 catcatgctg atcgtcggct gggccctggt cggcttcgct tcctacgcgc tgatcagcta
     1441 ctggtaccgc cggaacacgg cgaccaaggc cggcatgaag gccttcgtga tcaacgtgat
     1501 cggcgacatc ggcctttacc tcgcggcgat cctgatcttc aagaacatcg gttcggtcga
     1561 ttacggcacg gtcttcgaga aggcgccgga agtgttcacc accaacgagt gggagatcgt
     1621 cgcgatctgc ctctgcctgc tggtcggcgc cttcgccaag tccgcccaga tgccgttcca
     1681 cacatggctc gccgacgcca tggaaggccc gaccccggtt tcctccctga tccacgcggc
     1741 gaccatggtc acggccggcg tctacctgat cgcccgctgc cactcgctct tcgagcttgc
     1801 tccgaccgcg gcggacgtct ccgcctgggt cgggctggtc acgctgctga tcgcgggcac
     1861 gatcgcgatc gcggtcaccg acctgaaaag gataatcgcc tactcgacca tgagccagat
     1921 cggctacatg ttcgtcgggg tcagtatcgg ggcctactcg gccgggatgt tccacctgat
     1981 gacccacgcc ttcttcaagg cactgctctt catggcggcc ggttcgttca tctcggcgat
     2041 ggcgaacatg caggacatcg acaggatgaa gggctttcgc aaggcgatgc cgttcaccgc
     2101 ggcgatgatg atcatcgggg ccctggccct ttccggcttc cccggcacct cgggctggtt
     2161 ctcgaaggac gagatcatcg acttcgcgca tttccgcggc ggcatgtacg actggatgtg
     2221 ggtcggaatg ctggtcggcg ccttcatgac cgcggtctac tcgttccgga tcatcttccg
     2281 gatcctgccc ggcccgccct gcgaggaggc cgagtacctc gaggagcacg gtcacgtgat
     2341 ccacggtgag ccgaagaacc cggccaccgg cgagctggag gacaccgagg tcggctaccc
     2401 cggcgaggag catcacatcg ccgagcaggc cggcgggatg aagttcgcga tggcggtgct
     2461 cggcgtgctg gccctgatcg ccggcgtgct tcagatcccc gggctcgacg acgtggtcac
     2521 caagttcctc gaaccgacct tcgagaactc ggtcttctcc cacatgcatc cgaccaccag
     2581 tgaatcctgg atcggcctcg gcgagg
//
LOCUS       DAOQFI010000232         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21145_length_2601_cov_2.153574, whole genome shotgun sequence.
ACCESSION   DAOQFI010000232 DAOQFI010000000
VERSION     DAOQFI010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21145_length_2601_cov_2.153574"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLE93_04965"
     CDS             complement(<1..713)
                     /locus_tag="PLE93_04965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH86435.1"
                     /translation="MNETVTSPDLEGLDEAAIDLRKRARKFVDEVLIPLEQEAELLHG
                     RLPDEAVRMVREKALAAGLTGGLHKPEHGGQGWSKVEWFLVEEQLGRSTNAISWHIPN
                     GYNVWDLASEHHVEKWLKPLLRGETRDAYAVTERNAGSDPSGIETRAVRTDGGFRING
                     EKWFVTSGDVADVLVVMANLEEDGELLPTLFVVEKSLGGVETIEDPGFTHSYPDGHPT
                     IRFVDVEVGEDDVIGGIGGG"
     gene            848..2056
                     /locus_tag="PLE93_04970"
     CDS             848..2056
                     /locus_tag="PLE93_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNH86436.1"
                     /translation="MKVGSGNGVKGSVSGGLPLSPDDVARALRPLEHASMLPPAAFVD
                     QAVFEWEQKHLFHDGWICLGHVSQVSEQGKFIRREVGPDSVVVIGGEGGRPHAFLNVC
                     RHRGARVIEEADGQVKRRLQCPYHAWSYALDGTLVAAPHMDGVEDFDKSCYGLIPVRM
                     ATIGGLVLVDLGGEAPEPDAHVGELIEHLDRYRTAELVSGGRIDYEVAANWKGIAENY
                     NECLHCPGVHPELNALSNYMSGEEMRGSGSWCGGSMFITAEDAATMGKDGGHAGNRAR
                     IEGLTEEDDKTILYFALFPNALVSMHPDYVMLHTLYPRAPGRTEVICEWFFERRAVEA
                     GDFDPSDAIEFWDQTNRQDWYVCELTQKGVGTTGYTAGRYSAEEGTVHEFDNLVAGKY
                     MEALNGKVKA"
     gene            2053..>2601
                     /locus_tag="PLE93_04975"
     CDS             2053..>2601
                     /locus_tag="PLE93_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH86437.1"
                     /translation="MSGNGASPGNGKVPSGLLAATGDPVNANPVAAKLGKLGMPEPLS
                     ALAAKDWDAVVIGAGHNGLTAAAYLARAGQKVLVLERREQIGGACTLEEPFGEGYLVS
                     PCAYVVGLLDNLVIDELQLRRRGLRFFTADPNLWVPFEDGTAFGQFLDDEKTKANLDA
                     LGVSEADQKGYWAYEELFDQTRI"
BASE COUNT          451 a          843 c          872 g          435 t
ORIGIN      
        1 ccgccgccga tcccgccgat cacatcgtcc tcgccgacct cgacgtcaac gaagcggatg
       61 gtcgggtggc cgtccggata ggagtgggtg aagccggggt cctcgatcgt ctcgactccg
      121 cccagcgatt tctcgaccac gaagagagtg gggaggagct ctccgtcctc ttcgaggttg
      181 gccatgacga ccagcacgtc ggccacgtcg cccgaagtca cgaaccactt ctctccgttt
      241 atccggaagc cgccgtcggt ccggaccgcc cgggtctcga tccccgaggg gtccgagccg
      301 gcattccgtt cggtcaccgc gtaggcgtcg cgggtctctc cgcgcaggag cggcttcagc
      361 cacttctcga cgtgatgctc agaagcgaga tcccagacgt tgtaaccgtt ggggatgtgc
      421 caggagatcg cgttggtcga acggccaagc tgctcctcga ccaggaacca ctcgaccttg
      481 ctccagccct ggccgccgtg ttcgggcttg tgaaggccgc cggtgagacc ggccgcgagg
      541 gctttttccc tgaccatccg aaccgcctcg tcgggcagcc ggccgtgaag aagctccgct
      601 tcctgctcga gcgggatcag aacctcgtcg acgaatttcc gggctctttt ccggaggtcg
      661 atcgccgcct cgtcgaggcc ctccagatcg gggcttgtga cggtttcgtt catgtactcc
      721 ctcgccgatc tcgtttggtc aagcggaatg tctccagagt acatgcgatt gactacaagg
      781 tcaaccggcg ctagtatcgc tgcaggcggg cccatcccga gcccgtcggc acaaccggag
      841 gatcgagatg aaggtgggca gcggcaacgg cgtcaagggc agcgtctccg gcgggctccc
      901 cctgagtccg gatgacgttg cccgggcgct acggcccctg gaacatgcct cgatgctgcc
      961 gccggccgcc ttcgtcgatc aggccgtctt cgagtgggag caaaagcatc tcttccatga
     1021 cggctggatc tgcctcggcc atgtctctca ggtaagcgag cagggaaagt tcatccgccg
     1081 cgaggtcggg cccgactcgg tggtcgtgat cggcggcgag ggcggccggc cccatgcgtt
     1141 cctcaacgtc tgccgccatc gcggcgcccg ggtgatcgag gaagcagacg gtcaggtcaa
     1201 gcgccggctc cagtgcccgt accacgcctg gtcctacgcg ctggacggga ccctggtcgc
     1261 ggccccgcac atggacgggg tcgaggattt cgacaagtcc tgttacggcc tgatcccggt
     1321 ccggatggcg acgatcggcg gcctggtcct ggtcgacctg ggcggcgagg caccggagcc
     1381 ggacgcccac gtcggcgagc tgatcgaaca cctcgaccgc taccgcaccg ccgaactggt
     1441 cagcgggggg aggattgatt acgaggtcgc cgcgaactgg aagggcatcg ccgagaacta
     1501 caacgaatgc ctccactgcc ccggagtcca cccggagctg aacgcgcttt cgaattacat
     1561 gagcggggaa gagatgcggg gttccggttc ctggtgcggg ggctcgatgt tcatcaccgc
     1621 cgaggacgcc gcgacgatgg gcaaggacgg tggccacgcc ggcaaccggg cgcgaatcga
     1681 gggactgacc gaagaagacg acaagaccat cctctacttc gcgctctttc ccaacgcgct
     1741 ggtctcgatg catcccgatt acgtgatgct gcacaccctt tacccgcgcg cgcccggccg
     1801 gaccgaggtg atctgcgaat ggttcttcga gcggcgcgcg gtcgaggccg gtgacttcga
     1861 cccctcggat gcgatcgagt tctgggacca gaccaaccgg caggactggt acgtctgcga
     1921 gctgacccag aagggggtcg gcaccaccgg ttacacggcc ggccgctact cggccgagga
     1981 agggacggtc catgagttcg acaacctggt tgccggcaag tacatggaag cgctgaacgg
     2041 aaaggtgaag gcatgagcgg gaacggggcg agccccggga acggaaaggt gccgtccggg
     2101 ctgctggcgg cgaccggtga tccggtcaac gccaatccgg tcgcggcgaa actgggcaag
     2161 ctggggatgc cggagccgct ctcggccctg gcggcgaagg actgggacgc ggtggtgatc
     2221 ggggccggac acaacggtct gaccgctgcc gcctatctcg cccgggccgg tcagaaggtg
     2281 ctggtactcg aacgccgcga gcagatcggt ggcgcctgca ccctcgaaga accgttcggc
     2341 gaggggtacc tggtcagccc ctgcgcctac gtggtcggcc tgctcgacaa cctggtcatc
     2401 gacgaacttc agctgcgccg gcgcggcctc aggttcttca ccgcggaccc caacctctgg
     2461 gttcctttcg aggacggtac cgccttcggt cagttcctcg acgacgaaaa gacgaaggcg
     2521 aacctggacg cgctcggggt gagcgaagcc gaccagaagg gttactgggc ctacgaggaa
     2581 ctcttcgatc agacccggat c
//
LOCUS       DAOQFI010000233         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21175_length_2598_cov_3.048368, whole genome shotgun sequence.
ACCESSION   DAOQFI010000233 DAOQFI010000000
VERSION     DAOQFI010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21175_length_2598_cov_3.048368"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /gene="purL"
                     /locus_tag="PLE93_04980"
     CDS             <1..1731
                     /gene="purL"
                     /locus_tag="PLE93_04980"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNH86438.1"
                     /translation="EIYFEPPYEQNPLVNAMCVGLARTDEMVRAAAAGAGNKVVLFGS
                     LTGRDGIGGASVLASAELDEGDDAKRPTVQIGDPFEESKLLECCQELLAEDLLVSLQD
                     LGAAGLTSSAGEMASAGGTGIDIDVGLVPLREADMEPFEIMVSESQERMLAVVEPGKV
                     EQVLALCTKWQTASAVIGEVTDSGTFRILKDGETVGEIRVEDLVDGCPLYDLDPGDPG
                     EWTYPNRETIPADASASDIALTLLASPSIASKRWAFEQYDSIVGSRTAHRAGAADAAV
                     LNIPESGRAIAVAIDGNGRRVACDPYQGTIEATLECAANLAVVGAEPLGVTNCLNFGN
                     PEKPTVAWQLGRSIDGLAEACRALEAPVVGGNVSLYNETPQGPIYPTPVVGMVGELPD
                     PSRVAGEPWREGDAIALVGPFDPSLAGSELEKQRGQLGKGLPEFEIAPVRQAIELVRE
                     AVRSGSIRTATDVSDGGIATALAELAIGSGVGCDAHLTALLAKRDCSAEDALFGEGPG
                     GFILAGEEAGLEKLGGRGVEIEVIGEVRGNEVIVTASDRRVEILVSEAEAAFESLEER
                     IESDQAGITH"
     gene            1858..>2598
                     /locus_tag="PLE93_04985"
     CDS             1858..>2598
                     /locus_tag="PLE93_04985"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNH86439.1"
                     /translation="MKPLHDPDTTESRRDLAAQVAKPMRQFLATESGSAGLLLLAGVA
                     ALIWSNAPFSDSYFSFWSTELSVKVGDLSIAHDLRYWIDEALMAMFFFVIGMEVRREM
                     AMGELTDRKRVLIAVMGGIGGMIFPALIYLAINSGSGHEHGWGMVIATDTAFVLGALA
                     IVGPKFPTQLRVFLLTLAIVDDIVAITVIGLFYSEALDYTAIAICVAALFLIWLYPRI
                     GVWRSGIYFATVGVAVIAAMESGFHATLA"
BASE COUNT          415 a          877 c          866 g          440 t
ORIGIN      
        1 gagatctact tcgagccgcc ctacgagcag aacccgctgg tcaacgcgat gtgcgtcggc
       61 cttgcccgca ccgacgagat ggtccgggcg gccgcggccg gagccggcaa caaggtcgtg
      121 ctcttcggtt cgctgaccgg tcgcgacggc atcggcggcg cctcggtgct ggcctcggcc
      181 gaactcgatg aaggcgatga cgcaaagcgc ccgaccgtcc agatcggcga ccccttcgag
      241 gagtcgaagc tgctcgagtg ctgccaggaa cttctggccg aagacctgct cgtttccctt
      301 caggacctcg gtgccgccgg gctcacctcc tcggccggcg agatggcttc cgccggaggg
      361 accgggatcg atatcgacgt cggactggtt ccgctccggg aagccgacat ggagcccttc
      421 gagatcatgg tttccgaatc acaggaacgg atgctggccg tggtcgagcc gggcaaggtc
      481 gagcaggtgc tcgcgctctg caccaagtgg cagaccgcct ccgcggtgat cggcgaggtc
      541 accgattccg gcaccttccg catcctgaaa gatggcgaaa cggtgggtga gatccgggtc
      601 gaggacctgg tcgacggctg cccgctctat gacctcgacc caggggaccc gggcgagtgg
      661 acctatccca accgcgaaac catccctgcc gatgcctcgg ccagtgacat cgcgctgacc
      721 ctgctcgcct cgccttcgat cgcctccaag cgctgggcct tcgagcagta cgactcgatc
      781 gtcggttcgc gcaccgccca ccgggccggt gccgccgacg ctgccgtcct caacattccc
      841 gagtccggcc gggcgatcgc ggtcgcgatc gacggcaacg gccgccgggt cgcctgcgac
      901 ccctatcagg gcacgatcga agccacgctc gaatgcgcgg ccaacctcgc cgtggtcggg
      961 gccgagccgc tcggcgtcac caactgcctc aacttcggca atccggagaa gccgaccgtc
     1021 gcctggcagc tcggtcgctc gatcgacggg ctggccgagg cctgccgcgc gctcgaggcg
     1081 ccggtggtcg gcggcaacgt ttcgctctac aacgagactc cgcagggccc gatctacccg
     1141 accccggtgg tcggcatggt cggcgagctg cccgatccgt cccgggtggc cggtgagccc
     1201 tggcgcgagg gtgatgcgat cgccctggtc ggcccgttcg acccctcgct ggccggttcc
     1261 gagctcgaga agcagcgcgg ccagctcggc aagggcctgc cggagttcga gatcgccccg
     1321 gtaaggcagg cgatcgaact ggtccgggaa gcggtccgtt ccggttcgat ccgcaccgcc
     1381 accgatgttt ccgacggcgg tatcgccacc gccctggccg aattggcgat cggctccgga
     1441 gtcggctgcg acgcccacct gaccgcattg ctcgccaagc gcgactgttc ggcggaggat
     1501 gccctcttcg gcgagggccc cgggggcttc atcctcgccg gcgaggaagc cgggctggag
     1561 aagcttggtg gccgcggcgt cgagatcgag gtgatcggtg aggtccgggg caacgaggtg
     1621 atcgtcaccg cctccgaccg ccgcgtggaa atcctcgtca gcgaagccga agccgccttt
     1681 gaatccctcg aggagcgcat cgagtcagat caggcgggca tcacgcatta agtagtcggt
     1741 gagctgcgct ccgcctccaa gtaaaacccg ccggttcaca gaaagaaatg acggatgaac
     1801 gggctccgac cgctggtgag accaacatgc tgacccggta ggttcaacag ggacctggtg
     1861 aagccccttc acgatcccga tacaaccgag agcaggcgcg atcttgcggc ccaggtcgcg
     1921 aagccgatgc gccagttcct ggcgaccgaa tccggcagcg ccgggctgct gctgctggcc
     1981 ggcgtcgccg cgctgatctg gtcgaacgca cctttcagcg actcgtactt cagcttctgg
     2041 agcacggaac tctcggtcaa ggtcggcgac ctctcgatcg cacacgacct gaggtactgg
     2101 atcgacgagg cgctgatggc gatgttcttc ttcgtgatcg gcatggaggt ccgacgggaa
     2161 atggcgatgg gcgagttgac cgaccgcaag cgggtgctga tcgccgtgat gggcggcatc
     2221 ggcgggatga tcttccccgc cctgatctac ctggcgatca actccggttc cggccacgag
     2281 cacggctggg gcatggtgat cgccaccgac accgccttcg tgctcggggc gctggcgatc
     2341 gtcggtccga agttcccgac ccagctgcgg gtcttcctgc tcaccctggc gatcgtcgat
     2401 gacatcgtcg cgatcaccgt gatcggcctc ttctattcgg aggcgctgga ctacaccgcg
     2461 atcgcgatct gcgtcgccgc gctctttctg atctggctgt acccgcggat cggggtctgg
     2521 cgatcgggga tctacttcgc gaccgtcggg gtggcggtga tcgcagccat ggaatccggc
     2581 tttcacgcga ccctggcc
//
LOCUS       DAOQFI010000234         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21230_length_2595_cov_2.200000, whole genome shotgun sequence.
ACCESSION   DAOQFI010000234 DAOQFI010000000
VERSION     DAOQFI010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21230_length_2595_cov_2.200000"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLE93_04990"
     CDS             complement(<1..399)
                     /locus_tag="PLE93_04990"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNH86440.1"
                     /translation="MLASESAAYHGAGTCTFYGTANSNQMLLEAMGLHVPGAAFVHPH
                     DGLREALTREAVRSVLAITKRRRFTPIGRLVDERAIVNAMAALLATGGSTNHLIHWVA
                     VARAAGILIDWSDFSELSSVVPLLARVYPNG"
     gene            370..1491
                     /locus_tag="PLE93_04995"
     CDS             370..1491
                     /locus_tag="PLE93_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH86441.1"
                     /translation="MIGRAFRREQLVAARQPLRGPLDHWAAHSQQPPQAPGTGAGALF
                     LAASHGQPAIRPAGGSRGTVDALVRCLEAAGGELIVNAPAAKVETAGGRATAVIAGGE
                     RYEASKAVISAIDARRLFLGLMDESQVPAPLLAEVRRIHFSQHNVSELKVDAVIGEVP
                     RVNGPDGMEKAFMLSANTGTDIARAFSSLGLGQLPERPPVMIAFPSTLESGWAPEGQG
                     VVWLSTFVPWTPAQGEWDQALLEEASELTWKTAEKALGWEMTAVEKKLTGPLQWVERH
                     GNPGANPNHIEMSIDQLMAMRPSVNLSGYSTPIEGLFLTGAGTHPGGGITGMPGRNAA
                     GVILEQLGLARRKRGEKLKAQAALIKDALRATRELRKNA"
     gene            1488..2498
                     /locus_tag="PLE93_05000"
     CDS             1488..2498
                     /locus_tag="PLE93_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNH86442.1"
                     /translation="MSKTGMDGSAGDPYAVADQFTSYQESALIGTAVEVGLPNLLYGS
                     PHNLEGITTALGTDPRGTMGLLSGLVTFGLVQREGETFSLTANGELLADEHPDSVAMI
                     ARKEWFFYKLWAEMPAAVRTGHTQTGAWRERLENDPDQAHDFLRALDDLCRLYGGDLP
                     ELAGLPQGPPARRLLDVGGGAGSHSANLVKAVEGLKATVLDLPGAEPVLRERHPELDF
                     VAGDIDLPRFGRPEGEQWDYVLVSNILHDRPAAENERIIREAAGLLAPGGSLVIYEWV
                     IDEGRTSPPGVASFTPMMVIENEGGWTWTESEIGEWIRSAGLEPEPISQGPGPISVIK
                     AK"
BASE COUNT          445 a          916 c          856 g          378 t
ORIGIN      
        1 gccattcggg tacacgcgcg ccagcagcgg cacgacgcta ctcaactccg agaaatcgct
       61 ccaatcgatc aggatgccgg ccgcacgtgc caccgccacc cagtggatca ggtggttggt
      121 gctgccgccg gtggccagca gcgccgccat cgcgttgacg atcgcgcgct cgtcgacgag
      181 gcggccgatc ggcgtgaagc ggcgccgctt cgtgatcgcg agcacgctgc gcaccgcctc
      241 gcgcgtcagc gcctcgcgca ggccgtcgtg agggtgcacg aaagccgcac cgggcacatg
      301 caggcccatc gcctcgagca gcatctggtt gctgttggcc gtgccgtaga acgtgcacgt
      361 gccggcaccg tgataggccg cgctttccga cgcgagcaac tcgtcgcggc ccgccagccc
      421 ctgcgcggcc cgctcgacca ctgggcggcc cactcccagc agccgccgca ggcacccggg
      481 accggcgccg gcgccctctt cctcgccgcc tcacacggcc agccggcgat ccgccccgcc
      541 ggaggctcaa ggggaaccgt cgatgcgctg gtccgctgcc tggaagctgc cggcggcgag
      601 ctgatcgtga acgcgccggc cgcgaaggtc gagaccgccg gcggccgggc caccgcggtg
      661 attgcgggcg gcgagcgcta cgaagcgagc aaggcggtca tctcggcgat cgacgcccgc
      721 cgtctcttcc tcggcctgat ggacgagagt caggtgccgg caccgctgct ggccgaggtg
      781 cgccggattc acttcagcca gcacaacgtt tccgaactca aggtcgatgc ggtgatcggc
      841 gaggtcccgc gggtcaacgg tcctgacggc atggagaagg ccttcatgct ctccgccaac
      901 accggcaccg acatcgccag ggctttctcc agcctcggtc tcggccaact gcccgaacgg
      961 ccaccggtga tgatcgcctt cccttcgacc ctggaatccg gctgggcccc ggaaggtcag
     1021 ggcgtggtct ggctttcaac cttcgtgccc tggacccccg cgcagggcga gtgggaccag
     1081 gccctcctgg aggaggcctc cgagctgacc tggaagacgg cagagaaagc gctcggctgg
     1141 gagatgaccg cggtcgagaa gaaactgacc gggccgctcc agtgggtcga gcggcacggc
     1201 aacccgggcg ccaatcccaa tcacatcgaa atgagcatcg accagttgat ggcgatgcgg
     1261 ccctcggtca acctttccgg ctacagcacc ccgatcgaag gtctcttcct gaccggggcc
     1321 ggtacccacc cgggcggcgg gatcaccggc atgccgggga ggaacgcggc cggggtgatc
     1381 ctcgagcagc ttggcctcgc caggcggaag cgtggtgaaa agctgaaggc ccaggcggcc
     1441 ctgatcaaag acgcgctgcg cgcaaccaga gaactgagga agaacgcatg agcaaaaccg
     1501 ggatggacgg cagcgcgggc gacccctatg cggtcgccga ccagttcacc tcctaccagg
     1561 agtcggccct gatcgggacc gcggtcgagg tcggcctgcc gaaccttctt tacggctcac
     1621 cccacaacct ggaggggatc acgaccgcgc tcggaaccga cccgcgcggc accatgggtc
     1681 tgctttccgg gctggtcacc ttcggcctcg tccagcgtga aggcgagacc ttcagcctga
     1741 ccgcgaacgg cgagctcctg gccgacgagc accccgactc ggtcgccatg atcgccagaa
     1801 aagaatggtt cttctacaag ctctgggccg agatgccggc cgcggtgcgg accggccaca
     1861 cccagaccgg cgcctggcgt gagcgactcg agaacgaccc cgatcaggcc cacgatttcc
     1921 tccgcgccct cgatgacctc tgccgcctct acggcggcga tctgccggag ctggccggcc
     1981 tgccgcaggg cccgcccgcc cgccgcctgc tcgatgtcgg cggcggtgcc ggttcgcatt
     2041 cggccaacct ggtcaaggcg gtcgaaggcc tcaaggcaac ggttctcgac ttgccggggg
     2101 ccgagccggt cctcagggaa cgccatccgg aactcgactt cgtcgccggt gacatcgacc
     2161 tgccccgttt cggccggccg gagggggagc agtgggacta cgtgctcgtc tcgaacatcc
     2221 tgcacgaccg tccggcggcg gagaacgagc gcatcatccg ggaagcggcc gggctgctcg
     2281 ccccgggcgg ctcactggtc atctacgaat gggtgatcga cgagggccgt acctcgccac
     2341 ccggcgtcgc ctcgttcacc ccgatgatgg tgatcgaaaa cgaaggcggc tggacctgga
     2401 ccgaatccga aataggcgag tggatccggt cagccggcct cgaacccgag ccaatcagcc
     2461 agggcccggg cccgatctcg gtaatcaaag ccaaataact gcaagtcggg cggggtccga
     2521 cccgccctcc atgtgaaacc cgccagccca cttaatccat aaatggatgg gctggctcta
     2581 tctggtggtt cttct
//
LOCUS       DAOQFI010000235         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21243_length_2594_cov_2.667192, whole genome shotgun sequence.
ACCESSION   DAOQFI010000235 DAOQFI010000000
VERSION     DAOQFI010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21243_length_2594_cov_2.667192"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLE93_05005"
     CDS             complement(<1..217)
                     /locus_tag="PLE93_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HNH86443.1"
                     /translation="MSVDLADVSKLDVQPGELPETMAAWVIRPETEGNEPNKAFQFED
                     IETPEPGAFEVIVRVMAAGVNFNNVWAG"
     gene            complement(376..852)
                     /locus_tag="PLE93_05010"
     CDS             complement(376..852)
                     /locus_tag="PLE93_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86444.1"
                     /translation="MRGIRVTAFALLAATAAIAGCGSSTHENEPRPPVPTVVSISVGK
                     DTIDAKAAIGRGVGEPGPRQPYLNQNRTAPQNQADRKAPAVVNFAIANLTDRATVLRM
                     QGEVSRTVALTPGGSGSFMMALPTGIYRFSSPASSDTALLNVGPSRVSSGGDLLLP"
     gene            complement(881..1930)
                     /locus_tag="PLE93_05015"
     CDS             complement(881..1930)
                     /locus_tag="PLE93_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH86445.1"
                     /translation="MSEVRRQGLGGGKLGADSPLFGGSGASTNGDGDPAGDRFREPDE
                     QPAWDPVPDDFAESREAAAAEANRAAEELLKLGDLRDLLPAAEPDRALDDWGRSETVV
                     SLMEPLLNFYYRYWFRVEVEGLENVPSEGGALIVSNHSGALPPDAPMIMQAIRNEHPA
                     PRPLYMLGEHWFKGYPGVSMLVNKMGLVPAHRENAQRLLHDEGRLAVVFPEGQKGTRK
                     VYWQRYKLRRFGRGGFVRTAIRARVPIVPVAVIGAEESAPIFAHFKLLQKLSGLIYFP
                     LTPSFPHFGLLAPLMYLPSKFRIRFMEPIDMAEFPVETIDDPAEIQVISERIRARIQH
                     QLEEMLDARESVWTG"
     gene            complement(1927..>2594)
                     /locus_tag="PLE93_05020"
     CDS             complement(1927..>2594)
                     /locus_tag="PLE93_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNH86446.1"
                     /translation="MYKIGRKVEVGETDRALFSLIGPAAVEVTGLTPGSENDFTETVI
                     AGAGCLVVVTALGLDVICDPGSSDAVRAQLEADRAVPVSAEAAEILRVERGRPRFGFD
                     MTEANMPAEAGIVDRAVSFTKGCYIGQEPVARLHYKGRPNRHLRGLRPGGPVAHGDPV
                     RLGDRQLGEVGTAVLSPASGRIALSILRKEAEPGSVVTVETEAGEVEAEVVELPFIEG
                     SGI"
BASE COUNT          418 a          845 c          895 g          436 t
ORIGIN      
        1 gaccggccca gacgttgttg aagttgacgc cggccgccat cacgcggacg atgacctcga
       61 aagcgccggg ctcgggggtt tcgatgtctt cgaactggaa ggccttgttc ggctcgttgc
      121 cctcggtctc ggggcggatg acccaggcgg ccatcgtctc gggcagctct cccggctgca
      181 cgtcaagttt gctcacatca gcgaggtcta cagacacttc gttcctctcg ggggtttcgg
      241 tgggacgtgg aagttccgcc gaatggtacc tacccgggca gtattcaagc ggagccgtcc
      301 catccattct ttgcttcggt gggacggcgg gtttcaatgg gagggccggc gaagaccggc
      361 ccgactgccg tgagatcagg gaaggagcag gtccccgccg gaggagaccc ggctggggcc
      421 cacgttcaga agggccgtgt cgctgctggc cggcgaactg aaccggtaga tgccggtcgg
      481 cagggccatc atgaaagagc cggagccgcc cggggtcagg gccacggtcc ggctgacctc
      541 gccctgcatg cgaagaactg tggcccggtc ggtcaggttc gcgatcgcga agttcacgac
      601 ggccggggcc ttgcggtcgg cctggttctg gggggcggtc cggttctggt tgaggtacgg
      661 ctggcggggc cccggctcgc cgactccgcg gccgatggcc gccttggcgt cgatcgtgtc
      721 cttgccgacc gaaatgctga ccacggttgg aaccggcggg cgcggttcat tctcgtgggt
      781 cgatgagccg cagccggcga tcgcggcagt tgcagccagc agggcgaagg ctgtgacccg
      841 gattcccctc acagcctgaa aactacctga ctgtgtggcg tcaaccggtc cagaccgatt
      901 cgcgcgcgtc gagcatctct tccagctgat gctggatgcg ggccctgatc cgttcgctga
      961 tcacctggat ctcggccgga tcgtcgatcg tctcgaccgg gaattcggcc atgtcgatcg
     1021 gctccatgaa acggatcctg aacttcgagg gcaggtacat caacggcgcg agcaggccga
     1081 aatgcgggaa cgaaggggtg agcgggaagt agatcagccc ggagagcttc tgaagaagct
     1141 tgaagtgggc gaatatcggc gctgattcct cggcgccgat cacggccacc ggcacgatcg
     1201 gcacccgggc ccgaatcgcg gtccgcacga acccgccgcg accgaaacgg cgaagcttgt
     1261 agcgctgcca gtagaccttg cgggtcccct tctgcccctc cgggaagacg accgcgagcc
     1321 ggccctcgtc gtggagcagc cgctgcgcgt tttcgcgatg ggcgggaacc aggcccatct
     1381 tgttgaccag catcgagacg cccgggtagc ccttgaacca atgctcgccg agcatgtaaa
     1441 gcggacgcgg ggccggatgt tcgttgcgga tcgcctgcat gatcatcggg gcatccggag
     1501 gcagggctcc ggagtggttc gagacgatca gggcgccgcc ttcggaaggg acgttctcga
     1561 ggccttccac ctcgacccgg aaccagtacc ggtagtagaa gttgagcagc ggttccatca
     1621 gggacacgac cgtttcggag cggccccagt catcgagggc ccggtcgggc tcggcggccg
     1681 ggagcagatc cctcaggtcg ccgagcttga gcagctcctc ggcggcgcgg ttcgcctcgg
     1741 cggctgccgc ttcgcgtgat tcagcgaaat cgtcgggaac cggatcccag gcgggctgct
     1801 catcgggttc acggaaccgg tcgccggccg ggtcgccatc accgttcgtc gaagcgccgg
     1861 atccgccgaa gagggggcta tcggcaccga gcttgccgcc gcccaggccc tgccggcgca
     1921 cctcgctcat atcccggacc cttcgatgaa gggtaactcg acgacttcgg cttcgacctc
     1981 gccggcttcc gtctcgaccg tgaccaccga gccgggctcg gcctccttgc gaaggatgga
     2041 aagcgcgatt cggcccgatg cgggggagag gaccgcggtg ccgacttctc cgagttgacg
     2101 atcgccgagc cggaccggat ccccgtgggc gaccggtccg ccgggcctca gcccccgcag
     2161 gtgacggttc ggccggccct tgtagtgaag ccgggcaacc ggttcctggc cgatgtagca
     2221 gcccttggtg aagctgaccg cacggtcgac gatcccggct tcggccggca tgttggcttc
     2281 ggtcatgtcg aacccgaagc ggggccggcc ccgctcgacc cggaggatct cggccgcttc
     2341 ggcggaaacc ggaaccgccc ggtcagcttc gagctgggct cggaccgcat ccgaggagcc
     2401 cggatcgcag atcacgtcca gcccgagcgc ggtaaccacg accaggcagc cggcaccggc
     2461 gatcacggtc tcggtgaagt cgttttcact tcccggcgtg aggccggtca cctcgaccgc
     2521 ggccggcccg atcagcgaga agagggcccg atcggtctcc ccgacctcga ccttgcggcc
     2581 gatcttgtac atcg
//
LOCUS       DAOQFI010000236         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21270_length_2592_cov_3.693733, whole genome shotgun sequence.
ACCESSION   DAOQFI010000236 DAOQFI010000000
VERSION     DAOQFI010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21270_length_2592_cov_3.693733"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PLE93_05025"
     CDS             <1..976
                     /locus_tag="PLE93_05025"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNH86447.1"
                     /translation="AFGIGTSEVEHVMATQTLVQNKPKTMRINYHGKLGEGVTSKDLI
                     LATIGKLSTSGMTGYVVEYAGEAIEALTMEQRMTVCNMTIEGGGKAGMIAPDETTFEY
                     MRGKPGVPGDFDAAVERWKQLPTDEGATFDSEINIDATEISPMVTWGTTPGMVIQVTD
                     TVPDPEKMDSPADKEAAERALQYMGLEAGTPMEEIRPERVFIGSCTNSRISDLREAAG
                     AIKGRKVADSVRAMVVPGSALVKAQAEEEGLDEIFRQAGFEWRGAGCSMCLGMNPDIL
                     IEGERCASTSNRNFEGRQGKGGRTHLVSPTMATAAAIEGHFVDIRNWS"
     gene            986..1549
                     /gene="leuD"
                     /locus_tag="PLE93_05030"
     CDS             986..1549
                     /gene="leuD"
                     /locus_tag="PLE93_05030"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNH86448.1"
                     /translation="MEPISTIEGEIAVLDRSDVDTDQIIPKQFLKRVERTGYGEFLFY
                     DWVRDGEIELEPHPILVSGRNFGSGSSREHAVWALHQFGFQAVIAPSFSDIFYSNSTK
                     NGFLPVELDEEKCQQIVEAGEAKIDLESQTVSWNGGEAGFEIDAEIKRRLLGGLDDIA
                     LTLQSEDEIEAFEAGPGGTYGPVTTSL"
     gene            1605..2393
                     /locus_tag="PLE93_05035"
     CDS             1605..2393
                     /locus_tag="PLE93_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH86449.1"
                     /translation="MSSDQESRPADWEADKYSRTATPQREWSEAVLDRLELSGDETVV
                     DAGCGSGNVTEDLLAKLPDGRVIGVDGSPSMIEEATARLGDDPRTSFICQDLAKLQLD
                     EQVDHVFSNATFHWIADHDALFRSLAGATRPGGSLIAQCGGRGNVAEVIDALGVVNRQ
                     APFAEIFGEYDDPWTFAGPEETEIRLIGAGYEDVECWLEERVAHPEDPRGFFEASFLA
                     PARQVLDDEQFTEYTDRLLAEMGQPASFNYVRLNMTAKRKNENE"
     gene            2386..>2592
                     /locus_tag="PLE93_05040"
     CDS             2386..>2592
                     /locus_tag="PLE93_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNH86450.1"
                     /translation="MSETRKIVVLAGDGIGPEIVGATRRVLDAVGSFEYEEHLIGGAS
                     IDEHGVALTDEVIEACKASDAVLLG"
BASE COUNT          510 a          808 c          876 g          398 t
ORIGIN      
        1 ggccttcggc atcggcacct ccgaggtgga gcatgtgatg gccacccaga ccctggtcca
       61 gaacaagccg aagacgatgc ggatcaatta ccacggcaag ctcggcgaag gcgtgacttc
      121 caaggacctg atcctcgcca caatcggcaa gctcagcact tccgggatga ccggttacgt
      181 ggtcgagtac gccggcgagg cgatcgaggc actgaccatg gagcagcgga tgaccgtctg
      241 caacatgacg atcgagggcg gcggaaaggc cggcatgatc gcccctgacg agacgacctt
      301 cgagtacatg cggggcaagc cgggcgtgcc aggggacttc gatgcggcgg tcgagcgctg
      361 gaagcagctg ccgaccgacg agggagcgac gttcgattcc gagatcaaca tcgacgcgac
      421 cgagatctcg ccgatggtca cctggggcac gaccccgggc atggtgatcc aggtcaccga
      481 cacggtgccg gacccggaga agatggactc gcccgcggac aaggaagcgg ccgagcgggc
      541 gctgcagtac atgggcctcg aagccggaac tccgatggag gaaattcgcc cggagcgggt
      601 cttcatcggc tcctgcacca actcgcgaat ctcggacctg cgggaagcag ccggcgcgat
      661 caagggccgc aaggtggccg acagtgtgcg ggcgatggtc gtcccgggct cggccctggt
      721 caaggcccag gccgaagagg aaggtctcga cgagatcttc cgccaggccg gattcgaatg
      781 gcgcggcgcc ggctgttcga tgtgcctcgg catgaacccg gacatcctga tcgagggcga
      841 acgttgcgcc tcgacttcga accgcaactt cgaaggccgc cagggcaagg gcggccggac
      901 ccacctcgtc agcccgacca tggccaccgc ggcagccatc gaaggccact tcgtcgacat
      961 ccgcaactgg agctaggagc tagcaatgga accgatcagc acaatcgaag gcgaaatcgc
     1021 cgttctcgac cgcagcgacg tcgacaccga ccagatcatc cccaagcagt tcctgaagcg
     1081 ggtcgagcgg accggttacg gcgagttcct cttctacgac tgggtgcgcg acggcgagat
     1141 cgaactcgag ccgcatccga tcctggtttc cggccggaac ttcggctcgg gatcctcgcg
     1201 cgagcacgcc gtctgggcgc tccatcagtt cggcttccag gccgtgatcg ccccgtcctt
     1261 ctcggacatc ttctattcga acagcaccaa gaacggcttt ctgccggtgg agctggacga
     1321 ggagaaatgc cagcagatcg tggaggccgg cgaggcgaag atcgacctcg agagccagac
     1381 cgtgagctgg aacgggggag aggccgggtt cgagatcgac gccgagatca agcggcgcct
     1441 gctcggcggg ctcgacgaca tcgccctcac ccttcagtcc gaggacgaga tcgaggcctt
     1501 cgaggccggc cccggcggga cctacgggcc cgtgacaaca tccctttagg gcgcgcgtcg
     1561 agcgaactga ttcagcgcgc ctgtctttgt agaggagatc aatcatgagc agtgatcagg
     1621 aatcccgtcc ggcggactgg gaggcagaca agtattcccg cacggcgact ccgcagcgcg
     1681 aatggtcaga agccgtgctc gaccgtctgg aactgagtgg cgacgaaacg gtggtcgatg
     1741 ccggctgtgg ttccggcaac gtgaccgagg acctgctggc gaagctcccc gacggccggg
     1801 tgattggcgt tgacggttcc ccgagcatga tcgaggaagc caccgcgcgc ctcggggacg
     1861 acccgcggac cagcttcatc tgccaggacc tggcgaagct ccagcttgac gagcaggtcg
     1921 accatgtctt ctcgaacgcc accttccact ggatcgctga ccatgacgcc ctgttccggt
     1981 cgctggccgg ggcgacccgg cccggcggca gcctgatcgc gcaatgtggc ggccgcggca
     2041 acgtggccga ggtgatcgac gcgctcgggg tcgtcaaccg gcaggcaccc ttcgcggaga
     2101 tcttcggtga gtacgacgac ccgtggacct tcgccgggcc ggaagagacc gagatccggc
     2161 tgatcggtgc cggttacgag gacgtggagt gctggctgga ggaacgggtc gcccatccgg
     2221 aggatccgcg gggattcttc gaagcttcgt ttctggcccc ggcccggcag gtcctcgacg
     2281 atgaacagtt caccgagtac acagaccgct tgctggccga gatgggtcaa ccagccagct
     2341 tcaactatgt tcgcctgaac atgaccgcga aaaggaagaa cgagaatgag tgagacccgg
     2401 aagattgtgg tgctggccgg cgacggtatc ggcccggaaa tcgtcggcgc gacccgtcgg
     2461 gtcctcgacg cggttggcag cttcgaatac gaggaacacc tgatcggtgg tgcctcgatc
     2521 gacgagcacg gggtcgccct gaccgacgag gtgatcgaag cctgcaaggc atccgacgcg
     2581 gtgctgctcg gc
//
LOCUS       DAOQFI010000237         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21305_length_2590_cov_3.652071, whole genome shotgun sequence.
ACCESSION   DAOQFI010000237 DAOQFI010000000
VERSION     DAOQFI010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21305_length_2590_cov_3.652071"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLE93_05045"
     CDS             complement(<1..450)
                     /locus_tag="PLE93_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86451.1"
                     /translation="MPGIRFRGILVSAAFAVLPVFAAPAVSSAGDDPGTSVLEVRFSR
                     SASLRGDEASLRAASEPALDRVEQLFERIGTISAEPLAAGVSQAESTALARRASRLSG
                     ARSTNMADWYRVEVPAGTEADALDALRNSGQVAYAGRAPVALPPPVTP"
     gene            complement(682..1059)
                     /locus_tag="PLE93_05050"
     CDS             complement(682..1059)
                     /locus_tag="PLE93_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86452.1"
                     /translation="MKDQTLRRAAAVALALTGVIHLVLAPEYMAEQAYIGLLFIGGGI
                     TTIALAAWLWVRSEPVAWVGAALVSAGMAAGFILSRTVGLPGFHEAEWELSGVVSVLL
                     EIGVVGLAAAALGSGGSARTATS"
     gene            complement(1155..2171)
                     /locus_tag="PLE93_05055"
     CDS             complement(1155..2171)
                     /locus_tag="PLE93_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNH86453.1"
                     /translation="MSTENRRYALAERPEGLIDEDTFTLETGPVPEIGEGEALVRVTW
                     ISIDPTNRTWIGEEPTYLPPVQIGETMRALGLGEVVESNNPNYPVGALVTGLTGWTDY
                     TVISDSNPLMVVPEDARSAPPAALLGMLGMTGCTAYFGMLEIGEPKEGETVVVSAAAG
                     AVGTVAGQLARLKGARVVGIAGGPEKCAWLIDELGFDAAVDYKADDWKEQLRAATPDG
                     IDVNFENVGGEIMEAVLLRLNINARVALCGLISGYNDDLRPLDPRIFGRLLVNRVRLQ
                     GFIILDYYARFGEAIRELSGWVAEGKLKSEQTVVEGFENLPDALNMLFKGENKGKLVV
                     SIAK"
     gene            2213..>2590
                     /locus_tag="PLE93_05060"
     CDS             2213..>2590
                     /locus_tag="PLE93_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNH86454.1"
                     /translation="MGKTRRDLLVDGTKVAAGLAAANALAPTGPFGAAQALADPKSKL
                     RRPGFGLAFKSLKDETFLPSIDLDGKLPQWLTGSLLRNGPALFEIGEEKFNHWFDGLA
                     MLHAFSFKGGKVSYRNRFLRSSQY"
BASE COUNT          431 a          850 c          864 g          445 t
ORIGIN      
        1 gggcgtgacc ggtggcggaa gggcgaccgg ggcccggccg gcataggcga cctggcccga
       61 gttgcggagc gcatcgaggg cgtctgcttc agtgcccgcc ggtacctcga cccggtacca
      121 gtcggccatg ttggtgctcc tggctccgga gagcctgctg gcccgcctcg ccaacgcggt
      181 cgactcggcc tggctgaccc cggccgcgag aggttcggcg ctgatcgtcc cgatccgctc
      241 gaagagttgc tcgacccggt caagggcggg ctcgctcgcc gctcgaagac tggcttcgtc
      301 gcctctcaga ctggcactcc ggctgaaccg cacttcgaga accgaggttc cagggtcgtc
      361 gccggcggag gaaaccgccg gtgcggcaaa gacgggcagg accgcaaaag cggcgctgac
      421 gagaatcccc ctgaacctga tgcctggcat cagacccatg ctcccacagt ggagccgcct
      481 tgccaaccaa ctagggaccg gtcggtgtcg cctgggccgg gcggttccgg ccttccgcta
      541 caagttgcgt tgctggaaac agggaaggat ccccgacctc cagttcgagg ccggggatcc
      601 gggcggcggc gggcggtggc cgggcgccgg gctgggatgc cggcgtcctg gcctgtcaac
      661 tggtttctcc ttccccctgg atcaggaggt cgcggtccgg gcgctgccgc cggagccgag
      721 cgccgctgcg gcgaggccga cgaccccgat ttcgagcagc accgagacga ctcccgagag
      781 ttcccactct gcttcgtgga acccgggcag gccgaccgtc cgggagagga tgaagccggc
      841 ggccattccg gcgctgacga gcgcggcacc gacccaggcg accggctcgc tgcgaaccca
      901 gagccatgcc gccagcgcga tcgtggtgat tccgccgccg atgaagagga ggccgatgta
      961 ggcctgctcg gccatgtatt cgggggcgag cacgagatgg atgacaccgg tgagggccag
     1021 ggccaccgcg gccgcgcgtc tcaaggtctg atctttcatg tgaatccttc cgtcagattg
     1081 actagggatt cagaccttga tctgccgcga tcagatcatc ttcaacgccg cgttagaagc
     1141 ttcttatcga ccgctcattt ggcgatgctg acgaccagtt tgcccttgtt ttcgcccttg
     1201 aagagcatgt tcagggcgtc gggcaggttc tcgaaacctt cgaccaccgt ctgctcggat
     1261 ttcagtttgc cctcggcaac ccagcccgaa agttcgcgga tcgcctcgcc gaagcgggcg
     1321 tagtagtcga ggatgatgaa gccctgcagc ctgacgcggt tgaccagcag ccggccgaag
     1381 attctcgggt cgagcgggcg caggtcgtcg ttgtaacccg agatcagccc gcagagggcc
     1441 acgcgggcat tgatgttgag acgcagcagg accgcttcca tgatctcgcc gccgacgttc
     1501 tcgaagttga catcgattcc gtccggggtg gccgcccgga gctgctcttt ccagtcgtcg
     1561 gccttgtagt caacggcggc gtcgaagccg agctcgtcga tgagccaggc gcatttctcc
     1621 ggaccgccgg cgatcccgac cacccgggcg cccttcagcc tggcgagctg gccggccacg
     1681 gtgccgaccg ctcccgccgc ggcggaaacc acgaccgtct cgccttcctt cggctcgccg
     1741 atctcgagca tcccgaagta ggcggtgcag ccggtcatgc cgagcatgcc gagcagggcg
     1801 gcgggaggag cggacctggc gtcttccggc accaccatga gcggattgga gtcactgatc
     1861 acggtgtaat cggtccagcc ggtcaggccg gtgaccaggg cgccgaccgg gtaattcgga
     1921 ttgttcgatt cgaccacttc gccgaggccc agggcccgca tcgtctcgcc gatctggacc
     1981 gggggcaggt aggtcggttc ttcaccgatc caggtccggt tggtcgggtc gatcgagatc
     2041 caggtgaccc ggaccagggc ctcgccctcg ccgatctccg ggaccggccc cgtctccagg
     2101 gtgaaggtgt cctcgtcaat caggccttcg ggccgctccg ccaatgcata acgccgattc
     2161 tccgtgctca tatagaagaa cctatagtgg agcaaggaga gagggagaag ctgtgggaaa
     2221 aacgcgtaga gacctgctgg ttgatgggac caaagtggcg gccggactgg cggcggccaa
     2281 cgcgcttgcg ccgaccggac ccttcggggc agcgcaggcg ttggctgatc cgaagagcaa
     2341 gctgcgccgg cccggtttcg gcctcgcatt caagtcgctg aaggacgaga ccttcctgcc
     2401 ctccatcgat ctcgacggca agttgccgca gtggttgacc ggcagtctgc tgcgcaacgg
     2461 cccggccctc ttcgagatcg gcgaggaaaa gttcaaccac tggttcgacg gcctcgcgat
     2521 gctccacgcc ttctccttca agggaggcaa ggtcagttac cgcaaccgct tcctgcgctc
     2581 cagtcagtac
//
LOCUS       DAOQFI010000238         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21329_length_2588_cov_2.733123, whole genome shotgun sequence.
ACCESSION   DAOQFI010000238 DAOQFI010000000
VERSION     DAOQFI010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21329_length_2588_cov_2.733123"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1319
                     /locus_tag="PLE93_05065"
     CDS             42..1319
                     /locus_tag="PLE93_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNH86455.1"
                     /translation="MNSQSSAGGPVGIVGLGYVGLPIAVAFAEAGRQVIGLDSDPVKI
                     EKINRAESYIEDIPSETLVALDGRLEATASYEALAGCDAVIICVPTPLTGSRESDLSY
                     LESAGESLARVLTPGQTVILESTTYPGTTRDLLASTLEAGSRVAGRDFHLAFSPERID
                     PGRTDFTVRTTPKLVGGLTPACTDKAVALYETICDRVIPLSGPEAAELSKLLENIFRS
                     VNIALVNELAQLCDRLGVDVWEVIDAASTKPFGFMRFEPGPGMGGHCLPVDPFYLAFK
                     AREHDFYPEFIELAGKINQSQPLFCVNRIERVLNANGKPVKGTKVLILGVSYKAGVGD
                     TRESPAIKIIRLLGELGADVRYHDPHVPALKEAGLTNTPDLDGALADADLSVIITAHP
                     EFDWAEVIGKSDLVFDLRGVTREFDDPGNVTRL"
     gene            1346..2092
                     /locus_tag="PLE93_05070"
     CDS             1346..2092
                     /locus_tag="PLE93_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86456.1"
                     /translation="MIRELKGAASSLIPWRRAPHSHWQTIKSDRATLALAVGTVTIAG
                     LVLAAQYSRLLSRRTHSPGTEGLIDSAPAAAVDTVGVAVEGYSATPNRELILFNLLTG
                     FLGSFSAVRMTTWAIREGWGPFRNVSIGGRHIHHFVPGILIGFGSGIAALLLNGEKAD
                     RRIARSLGVGMGLTFDEAALLLDMQDVYWTRQGLLSVQVTLATGATLGATILTMRILG
                     RGETRQEEAGEIPAPEGQMNTVVPWPHPAM"
     gene            complement(2389..>2588)
                     /locus_tag="PLE93_05075"
     CDS             complement(2389..>2588)
                     /locus_tag="PLE93_05075"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNH86457.1"
                     /translation="SILHNATPEYVDLPGFSEDITGCRTEEDMPLAAREYMAFVSEFV
                     GVPINLVGVGPGRDEVVWFDG"
BASE COUNT          462 a          859 c          825 g          442 t
ORIGIN      
        1 acgtacctga agaactggcc ccaggttcta gactccgcag cgtgaactcg cagtcttcag
       61 ccggcggtcc ggtcggaatc gtcggcctgg gatacgtcgg cctgccgatc gccgttgcct
      121 tcgccgaagc cggccggcag gtgatcggcc ttgacagcga cccggtcaag atcgagaaga
      181 tcaaccgggc cgagagctac atcgaggaca ttccctcgga aaccctcgtc gccctggatg
      241 gccgtctcga ggcgaccgcg agctacgaag cgctggccgg atgcgatgcg gtgatcatct
      301 gcgtgccgac cccgctgacc ggttcgcgcg agtccgacct cagctacctg gaatcggccg
      361 gcgagagcct tgcccgggtg ctgactcctg ggcagaccgt gatcctcgaa tcgaccacct
      421 accccggcac gacccgcgac ctgctggcga gcacgctcga agcaggcagc cgggttgcag
      481 gccgggattt tcacctcgcc ttttccccgg aacggatcga tccgggccgc accgacttca
      541 cggtccgcac caccccgaaa ctggtcggtg gtctcacccc cgcctgtacc gacaaggcgg
      601 tggcgcttta cgaaacgatc tgcgaccgcg tgatcccgct ctcgggccct gaggctgcgg
      661 agctttcgaa gctgctggag aacatcttcc gctcggtcaa catcgcgctg gtcaacgaac
      721 tggcacagct ctgcgaccgg ctcggcgtag acgtctggga ggtgatcgat gccgcttcga
      781 ccaagccctt cggtttcatg cggttcgagc cgggccccgg catgggcggt cactgcctgc
      841 cggttgatcc cttctatctc gccttcaagg cacgcgagca tgacttctac ccggagttca
      901 tcgagctcgc cggcaagatc aaccagtcac agccgctctt ttgcgtcaac cggatcgagc
      961 gggttctcaa tgcgaacggc aagccggtca aaggcaccaa ggtcctgatc ctcggcgtca
     1021 gttacaaggc cggggtcggc gacacgcgcg agtcgcccgc gatcaagatc atccggctgc
     1081 tgggtgagct gggcgcggac gtgcgctacc acgacccgca tgtgccggcc ttgaaggagg
     1141 ccggactgac caataccccc gacctcgacg gcgccctcgc cgacgccgac ctcagcgtga
     1201 tcatcaccgc ccacccggaa ttcgactggg cggaggtaat cggaaagagt gacctggtct
     1261 ttgacctgcg cggggtcacc cgggagttcg atgatcccgg caacgtgacg aggctctgac
     1321 cgggccggga agaaggagcg cgggcttgat tcgggaacta aagggcgccg cgtcgagcct
     1381 gatcccctgg cgacgggcgc ctcactctca ctggcagacg atcaagagcg accgggcgac
     1441 cctcgccctg gccgtgggca cggtgacgat cgccggactg gttctcgccg cgcagtactc
     1501 gcggctgctt tcccgccgca cccattcacc cgggaccgag ggtctgatcg attcggcccc
     1561 ggcggccgcg gtcgacacgg tcggggtcgc ggtcgagggg tactcggcca ccccgaaccg
     1621 ggagctgatc ctcttcaacc tgctgaccgg ttttctcggc agcttctccg cggtccggat
     1681 gaccacctgg gcgatccggg aaggctgggg ccctttccgc aacgtcagca tcggcggccg
     1741 ccacattcac catttcgtgc cggggatcct gatcgggttc ggctccggca tcgctgccct
     1801 gctcctgaac ggcgagaagg ccgaccggcg aatcgcccgc agcctcgggg tcgggatggg
     1861 cctcaccttt gacgaggcgg ccctgctgct cgacatgcag gacgtgtact ggaccaggca
     1921 ggggctgctt tcggtacagg tgaccctggc caccggggct accctcgggg cgacgatcct
     1981 gaccatgcgg atcctcggcc gcggcgaaac ccgccaggag gaggcgggtg agatcccggc
     2041 ccccgaggga cagatgaaca cggttgtacc gtggcctcac ccggctatgt agacagccac
     2101 gtaccgagac gcaacgtttt tgtaatttgt ccgcccggcc cgctccctgg agcgcaattc
     2161 ccttgttcaa gggggattcg aggcataaaa aatgtcggcg gtcgggctga cccaagggag
     2221 agtgggccaa cccgacttca ccgactaagc gctgctgagg gtagggaggg accccagtca
     2281 acgatgtttt gcagttatca cgtccagtga tctaactgct gatacgacat agtacaccaa
     2341 acacacgcgt ttatggcgaa ccgccgaaaa aagatttcct aaagggggtc agccgtcgaa
     2401 ccagacgacc tcgtcccggc cgggaccaac cccgacgagg ttgatcggga cccccacgaa
     2461 ctcgctcacg aaggccatgt actcccgggc cgcgagcggc atgtcttcct cggtccgaca
     2521 gccggtgatg tcctcggaga agccgggcag gtcgacgtac tcgggggtgg cgttgtggag
     2581 gatcgact
//
LOCUS       DAOQFI010000239         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21423_length_2582_cov_3.097744, whole genome shotgun sequence.
ACCESSION   DAOQFI010000239 DAOQFI010000000
VERSION     DAOQFI010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21423_length_2582_cov_3.097744"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLE93_05080"
     CDS             <1..499
                     /locus_tag="PLE93_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH86458.1"
                     /translation="LLDIEMPNVNGIESMRRILSRFPEIRILVYTAHDDPSLVRLASR
                     EGASGFLVKSADLDQIRDACETVMAGERWFPGYTDKDLQQEDELKRFMELSDREREVL
                     ALLATGMRAQGAAEEIGIRTATVYTHVRNVVNKLGVDTRTQAVAIATRYAFLDRDPEK
                     PPTAG"
     gene            complement(574..978)
                     /gene="msrB"
                     /locus_tag="PLE93_05085"
     CDS             complement(574..978)
                     /gene="msrB"
                     /locus_tag="PLE93_05085"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNH86459.1"
                     /translation="MSSDSTPLSRSDAEWRESLSPEAYEVTRNAATERPFTGPHLDNK
                     QDGSYTCVCCGAELFSSDAKFDSGSGWPSFTEPANLEHVELRQDNSHGMVRTEVVCRN
                     CDAHLGHVFPDGPAPTGQRYCINGCALEFNEK"
     gene            978..1583
                     /locus_tag="PLE93_05090"
     CDS             978..1583
                     /locus_tag="PLE93_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNH86460.1"
                     /translation="MFELSFISPVSRLAKQSSLLAAFLVAACLLVGVNGQLGAKPADA
                     ASLIAPAKTCPNRAASGGKVAMAKARKSMICMVNYARGREGLRPYRTRQQLTWSSRHK
                     ARDILRCGFSHEACGRSFDFWIKRSGYLGEGGWATGENIAWGSGSLGDVRSIFIAWMK
                     SKGHREAILSRTFTDVGTGVVKGTFQSFHGARIWVLHFGDN"
     gene            1713..>2582
                     /locus_tag="PLE93_05095"
     CDS             1713..>2582
                     /locus_tag="PLE93_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH86461.1"
                     /translation="MPEKSELDVLLKTEQTFPPPEGFAAASEFNDPGIYEQADADPEG
                     WWASWAEKLDWIEPWDTILDWSNPPHVTWFAGGKLNVSANCLDRHIEAGRADDIAYHW
                     EAEDAGPDDEPVTGVGSHAGGRISVTYGWLHDQTQRLANVLRDRGVGKGDVVGIFMPM
                     VPEAAVAMLACTRIGAIHNVVFGGFSAESLRERMEFSDAKALITADATLRRGNPMPMK
                     AAIDEALDTLPAMEHVIVLDRCGTGAPLKEGRDLDWAEALAAADPVCEPEPMDAEDPL
                     FLLYTSGSTAKPKG"
BASE COUNT          473 a          836 c          845 g          428 t
ORIGIN      
        1 cctgctcgac atcgagatgc ccaacgtcaa cggcatcgag tcgatgcggc ggatcctctc
       61 ccgcttcccc gagatcagga tcctcgtcta cacggcgcat gacgacccga gtctggtgcg
      121 cctggccagc cgggagggcg ccagtggttt tctggtcaag tcggccgacc tcgaccagat
      181 cagggacgcc tgcgaaaccg tcatggccgg tgaacggtgg ttccccggtt acaccgacaa
      241 ggacctgcag caggaagacg agctgaagcg cttcatggaa ctctccgacc gggagcggga
      301 agtcctcgcc ctgctggcaa ccgggatgcg ggcccaggga gcggcggagg aaatcggcat
      361 ccggaccgcg accgtctata cgcatgtgcg caacgtcgtc aacaagctcg gggtggacac
      421 ccggacccag gcggtcgcga tcgcgacccg ctacgcgttc ctggaccggg accccgaaaa
      481 gccgcctacc gctggttaaa tcggaacaac gggtcgaatt cagtcacgtt cacttcttgt
      541 tgaatcggaa caaggggtgg aattcagtca cattcacttc tcgttgaatt ccaaagcaca
      601 cccgttgatg cagtagcgct ggccggttgg ggctggtccg tcggggaaga cgtggccgag
      661 gtgggcgtcg caattgcggc agacgacctc ggtccgcacc atgccgtgac tgttgtcctg
      721 cctcagctcg acatgttcga ggttggcggg ttcggtgaaa ctcggccagc cgctgcccga
      781 atcaaacttc gcgtcggagg agaagagctc tgccccgcag cagacgcagg tgtaggaccc
      841 gtcctgcttg ttgtcgaggt gagggccggt gaaaggccgt tcggtcgcgg cattgcgggt
      901 gacctcatag gcctcggggg aaagactttc ccgccactcc gcatcactcc tgctgagagg
      961 tgttgaatca ctagacattg tttgagctct ctttcatttc gcctgtttcc agactagcca
     1021 agcagtcaag cctgcttgcc gcttttctgg tcgcagcctg ccttctggtt ggcgtcaacg
     1081 gccagctcgg agccaaaccg gccgatgccg ccagcctgat cgcgccggcc aagacctgcc
     1141 cgaaccgcgc cgccagcggt ggcaaggtcg cgatggccaa ggcccgcaag tcgatgatct
     1201 gcatggtcaa ctacgcccgg ggccgggaag gcctccgccc ctaccggacc cggcagcagt
     1261 tgacctggtc atcccgccac aaggccaggg acatcctgcg ctgcggtttc tcgcatgaag
     1321 cctgcggaag gagctttgac ttctggatca agcggtccgg ctatctcggc gaaggcggct
     1381 gggcgaccgg cgagaacatc gcctggggca gcggatcgct cggcgacgtg cgctcgatat
     1441 tcatagcctg gatgaagtcg aaggggcatc gggaagcgat cctcagccgg accttcaccg
     1501 acgtcgggac cggcgtcgtc aagggaacat tccagtcttt ccacggggcc cggatctggg
     1561 tcctgcattt cggcgacaac tgaccgtcgc ccttcgcccc gttccgggct gcctcgaagc
     1621 agtgcccgaa ccgatcctcc ggaccgggag tccggcgggg gctagcattg ggccaccgag
     1681 gagaaccggc cccgaccccg gaggagagat cagtgccaga gaagtcagag cttgacgtcc
     1741 ttctgaagac cgagcagacc ttcccgcccc ccgaagggtt cgctgcggcg agcgagttca
     1801 atgaccctgg gatctacgag caggccgacg ccgaccccga aggctggtgg gcgagctggg
     1861 cggagaagct cgactggatc gaaccctggg acacgatcct cgactggagc aacccgcccc
     1921 acgtgacctg gttcgccggc ggcaagctca acgtcagcgc caactgcctc gaccgccaca
     1981 tcgaggcggg ccgggcagat gacatcgcct accactggga ggccgaagat gccggcccgg
     2041 acgacgagcc ggtgaccgga gtcggcagcc acgcgggcgg gcgtatctcc gtgacctacg
     2101 gatggctcca cgaccagacc cagcgactcg caaacgtgct cagggaccgt ggcgtcggca
     2161 agggtgacgt ggtcggcatc ttcatgccga tggtgcccga ggcggccgtg gcgatgctgg
     2221 cctgcaccag gatcggggcg atccacaacg tcgtcttcgg cggcttctcg gccgagtccc
     2281 tgagggaacg gatggagttc tcggacgcga aggccctgat caccgccgat gccaccctgc
     2341 gccgcggcaa tccgatgccg atgaaggcag cgatcgacga ggccctggac acgctgccgg
     2401 cgatggagca cgtgatcgtc ctcgatcgct gcgggaccgg cgctccgctc aaggaaggcc
     2461 gcgaccttga ctgggcagag gccctcgccg ccgccgaccc ggtttgcgaa ccggagccga
     2521 tggacgccga ggatcctctc ttcttgctct acacctcggg gtcgaccgcg aagccgaagg
     2581 gc
//
LOCUS       DAOQFI010000240         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21677_length_2564_cov_3.683141, whole genome shotgun sequence.
ACCESSION   DAOQFI010000240 DAOQFI010000000
VERSION     DAOQFI010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21677_length_2564_cov_3.683141"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="PLE93_05100"
     CDS             complement(<1..1330)
                     /locus_tag="PLE93_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNH86462.1"
                     /translation="MTPEATPPVGPVVPAAGTVPGNGAGIGSAAGPSAIETADLTARE
                     RSLLDDLMAVIQQFEADLATHGSSPAATIDRDEVTRAFVFACRHHADQKRKSGDEFIT
                     HPVGVARICVGMALDTETLCAALLHDTVEDTSASLEEIELEFSPTVSRLVDGVTKLTE
                     ITFESRDERQAENYRKMMVAMATDVRVILIKLADRLHNMRTLSSLPKQKQILKSRETL
                     EIYAPLAHRLGIHAIKWELEDLAFATLHPRKYSEIKHLVAQQRTERENFVEEAGEYLS
                     DELEKVGIEAEIAGRAKHFYSIYTKMTKKGREFNEIFDLTAMRVIVGSVKDCYGAIGV
                     IHSLWKPLPGRFKDYVAMPKANMYQALHTTVIGLEGKPLEIQIRTAEMHRLAEYGIAA
                     HVAYKEGGSGTDSSREKMTWLRQLVESESEQDPTEFLQSLKEDLFEDEVFV"
     gene            complement(1327..>2564)
                     /locus_tag="PLE93_05105"
     CDS             complement(1327..>2564)
                     /locus_tag="PLE93_05105"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86463.1"
                     /translation="GRAVASRIGTEPAEKAIEFDAVAGGADLGLDLAEEIEKLQPFGN
                     GNPPVRLFLPGARIEDLQEMGEGKHCRFAVCSGSHRATGVAFGRTSFGLAEGERADVI
                     AELGINHWNGSIEPQLRVTEVSRLPAAEPLAECEPDEWWQRFETALTRPLEGAGPAEK
                     AEVRIASGPLGLPGVALGELISSGERIVVLTADARQRWVGLGGAALGRFSSRDGEEAV
                     GVWAGSPASVSEPARDASVLLTDFTTLAGANPPVIDDFDRIALLDPPYLAASVAELAR
                     LDLPVHLLAGPVEFEFTARVAAYHLDPTVQLRNLFRRFREADAIDGQATEGEPLRAVL
                     SEDGNSTRSPEQAATLLRILVETGLARTEGEGSARRAGVVSSVKADLSVSVVFSHHIE
                     LHQEHRGFLSQFDRQTQTQ"
BASE COUNT          410 a          858 c          838 g          458 t
ORIGIN      
        1 agacgaatac ctcgtcctcg aagagatcct ccttcagcga ctggagaaac tcggtcggat
       61 cctgctcgga ctccgactcg accagctgcc gcagccaggt catcttttcg cgggaggaat
      121 cggtgccgct gccgccttcc ttgtaggcga catgggcggc gatcccgtac tccgccagac
      181 gatgcatttc cgccgtccgg atctggatct ccagcggctt gccctcaagg ccgatcaccg
      241 tcgtgtgaag tgcctggtac atgttcgcct tcggcatcgc gacgtaatcc ttgaagcgac
      301 cgggcagggg cttccagagc gaatggatca ccccgatcgc gccgtaacaa tccttgaccg
      361 agccgacgat cacccgcatc gcggtcaggt cgaagatctc gttgaactcc cggcccttct
      421 tggtcatctt cgtgtagatc gagtagaagt gcttggcgcg gccggcgatt tcggcttcga
      481 tgccaacctt ctccagctcg tccgacaggt actcgcccgc ctcctcgacg aagttctcgc
      541 gctcggtccg ctgctgggcg accaggtgct tgatctccga gtacttgcgc ggatgaagcg
      601 tcgcgaaggc gaggtcctcg agttcccact tgatcgcgtg gataccgagc cggtgggcca
      661 gcggcgcgta gatctccagc gtctcgcggg acttgaggat ctgcttctgc ttgggcagcg
      721 aactcagggt ccgcatgttg tgaaggcggt cggcgagctt gatcaggatc acccgcacgt
      781 cggtcgccat cgccaccatc atcttgcggt agttctcggc ctgcctctcg tcacggcttt
      841 cgaaggtgat ctcggtcagt ttggtgacgc catcaaccag gcgggaaacg gtcgggctga
      901 actccagctc gatctcctcg aggctggccg aggtgtcctc gaccgtgtcg tggagcaggg
      961 ccgcgcagag tgtctcggtg tcgagcgcca tgccgacgca gattcgggcg accccgaccg
     1021 gatgggtaat gaactcgtcg ccggatttac gcttctggtc ggcgtggtgc cggcaggcga
     1081 agacgaaggc gcgagtcact tcgtcccggt cgatggtcgc ggcgggagat gacccgtggg
     1141 ttgcgaggtc ggcctcgaac tgctggatga ccgccatcag gtcgtcgagc agggatcgtt
     1201 cgcgagcggt cagatcggca gtctcgatcg ccgacgggcc ggctgcggag ccaatgccgg
     1261 ctccgtttcc gggcacggtt ccggctgcgg gcacgaccgg accgaccggc ggcgtcgcct
     1321 ccggtgtcac tgcgtctggg tctgcctgtc gaactggctg agaaatcctc tgtgctcctg
     1381 atgaagctcg atgtgatgtg agaaaacgac cgaaacggaa agatccgcct tcaccgagga
     1441 tacgaccccg gcccttcggg cactcccctc gccttcggta cgggcgaggc ctgtctcgac
     1501 cagaatccgc agcagagtcg ccgcctgctc cggcgaacgg gttgagttgc cgtcttcgga
     1561 gaggaccgcc cggaggggct ccccttcggt cgcctgaccg tcgattgcgt cagcttcgcg
     1621 aaagcggcgg aagaggttcc tgagctgcac ggtcgggtcg aggtgatacg ccgcgacccg
     1681 ggccgtgaac tcgaactcga ccggcccggc aagaagatga accggcaggt cgagcctggc
     1741 cagttccgcc acgctcgcgg ccagatacgg cgggtcgagc agggcgatgc ggtcgaagtc
     1801 gtcgatcacc gggggattgg cgccggccag ggtcgtgaag tcggtgagca ggaccgacgc
     1861 atcgcgggcc ggctccgaaa cggatgccgg gctgccggcc cagaccccga ccgcctcctc
     1921 cccgtcgcgt gaagaaaagc ggcccagggc ggcgccgccg agaccgaccc agcgctggcg
     1981 cgcatcggcc gtgagcacga cgatccgctc tccggagctg atcagctcgc cgagcgccac
     2041 acccggcagg ccgagcggcc ccgaggcgat tctgacctcc gccttttcag ccggcccggc
     2101 tccctccagg ggcctcgtca gtgcggtctc gaagcgttgc caccactcgt ccggctcaca
     2161 ctccgcgagc ggttcggcgg ccgggagccg ggaaacctcg gtgacccgca gctgcggttc
     2221 gatcgagccg ttccagtggt tgatccccag ttcggcgatc acgtcggccc tttccccttc
     2281 tgccagtccg aacgaggtcc ggccgaaggc cacgccggtt gcgcgatggg agcccgaaca
     2341 gacggcgaag cggcagtgct tcccttcgcc catctcctgc agatcctcga tcctggcacc
     2401 gggcaggaaa agcctgaccg gcggattgcc gttcccgaac ggctgcagct tctcgatctc
     2461 ctcggccagg tcgagcccca ggtcggcacc cccggccacg gcgtcaaact cgatcgcctt
     2521 ctcggcgggt tcggtgccga tccgtgacgc gaccgcccgg ccca
//
LOCUS       DAOQFI010000241         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21729_length_2561_cov_2.832801, whole genome shotgun sequence.
ACCESSION   DAOQFI010000241 DAOQFI010000000
VERSION     DAOQFI010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21729_length_2561_cov_2.832801"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLE93_05110"
     CDS             <1..499
                     /locus_tag="PLE93_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HNH86464.1"
                     /translation="TMGKLDFDDLQSVEGYNRWRAYGQSKLANLVFAMDLQSLIRQAG
                     LDMASIAAHPGVSNTNLTAAGNDLDSGWLGLLSKPFLALSDLLIAQDAAHGALPTLFA
                     ATEPGLTGGEYIGPDGLRQYRGSPTIVAPRRLALDSGTGDRLWKASTELTGVEYDFSP
                     ASPAA"
     gene            complement(517..2208)
                     /locus_tag="PLE93_05115"
     CDS             complement(517..2208)
                     /locus_tag="PLE93_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNH86465.1"
                     /translation="MTSVLRKAGLTGLLAALALAIALPAAASADTQRFYDNWAVGSGP
                     IGIANDRFGDVYVSSNTPAPFLSRYTAEGEFVASFATGGAILGARDVTVDSAGNVYQV
                     NATNNMVRKFSPDGTLITGWGTSGSGDGQFLDPNALATGPDNRIYVGDFGNDRVQWFT
                     PAGVYLGQFATGPMAGLDVGADGNVFVADSLSDEIRVYDPSGTPLDTVGENGSGAGDI
                     DNVSDVDIGDDGRIYAADTSSGMIRVYAPDGSYQGSLGSPGAGDGELNLPNALTADRA
                     GNVWTTQYGGSPRVSLFAYAPRVIGGTNLDFGYSFIGNPPGVQTVLMQNDNYVLPMYV
                     GTASLTSGVDFGLPAPWFECSGVILLPGQVCSVGVDFHPATPGNKTDTLNLDGGWRQV
                     TLSGTGISGPTGPTGATGSTGSTGSSGATGATGSTGATGATGPTGPKGPTGATGPKGP
                     SGNNATPRVNRIHTVVRVGAGPVAMVSVHCPKASCTIWQRQGRARSRGRVIQTRVIGP
                     NRIAAGGTARFRVTVPPRIRNLLTRQRSGTVNVYLAVQADKGNSIRRNVRIGIRR"
     gene            complement(2282..2434)
                     /gene="ssrS"
                     /locus_tag="PLE93_05120"
     ncRNA           complement(2282..2434)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLE93_05120"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          425 a          860 c          792 g          484 t
ORIGIN      
        1 caccatgggc aagctcgatt tcgatgacct gcagtcggtc gagggctaca accgctggcg
       61 ggcctacggc cagtcgaagc ttgccaatct ggtcttcgcg atggacctgc agtcgctgat
      121 ccgccaggcc ggtctcgata tggcgagcat cgccgcccac cccggtgtct cgaacaccaa
      181 cctgaccgcg gccggcaatg acctcgactc gggctggctc ggacttctct caaagccgtt
      241 cctcgctctt tccgatctgc tgatcgccca ggatgccgca cacggcgccc tgcccaccct
      301 ctttgccgcg accgagcccg gtttgacagg cggcgagtac atcggcccgg acggtctgcg
      361 ccagtaccgc ggttcaccca ccatcgtggc cccgcgcagg ctcgcgctcg attccgggac
      421 aggcgatcgc ctgtggaagg cgtcaaccga actgaccggg gtcgaatacg acttctcccc
      481 ggccagtccg gctgcctgaa tcccggcctc tcgctgctac cgcctgattc cgatccggac
      541 gttcctccgg atcgagttgc ccttgtcggc ctgtaccgcc aggtagacgt tgacggttcc
      601 cgaccgctgg cgggtcagca ggttccggat ccggggcgga accgtgaccc ggaaacgggc
      661 cgtgccacct gcggcgatcc ggttcggccc gattaccctg gtctgaatga cgcggccgcg
      721 ggaacgggcc cggccctgtc gctgccagat cgtgcaggag gccttcggac aatggacgct
      781 gaccatcgct accggaccgg cgccgacccg gacgaccgtg tgaatacggt tcacccgggg
      841 agtggcgttg ttgccacttg ggcccttggg gccggtggct ccggttggcc ctttcggccc
      901 ggtcggcccc gtcgcgccgg ttgctccggt cgaacccgtg gcgccagtcg cgcccgaact
      961 gccggttgag ccagtagagc cggtcgcgcc agtaggcccg gttggcccgc tgatcccggt
     1021 cccgctcagg gtgacctgcc gccagccgcc gtccaggttg agtgtgtcgg tcttgtttcc
     1081 cggtgtggcc ggatgaaagt cgaccccgac gctgcacact tggcccggga gcaagatcac
     1141 gccagagcac tcgaaccagg gtgccgggag tccgaagtcg accccggatg tcaggctcgc
     1201 cgtcccgaca tacatcggca ggacgtaatt gtcgttctgc atcagcaccg tctggactcc
     1261 gggagggtta ccgatgaagg aatacccgaa gtcgaggttc gtacctccga tcacccgcgg
     1321 cgcgtatgcg aacaggctga cccggggcga tccgccgtac tgcgtcgtcc agacattgcc
     1381 ggcacggtcc gcggtaagtg cgttgggaag gttcaactct ccgtcgccag cccctgggga
     1441 tccgagcgag ccctgatagg acccgtccgg cgcatagacc ctgatcatgc cgctggaagt
     1501 gtcggcggcg tagatgcgac cgtcgtcgcc gatgtcgacg tcgctcacat tgtcgatgtc
     1561 acccgcgccc gagccgtttt ctccaacggt gtcgagcgga gttccggacg ggtcgtagac
     1621 ccggatttca tcgctcagcg aatcggccac gaatacgttt ccatcggctc cgacatcgag
     1681 gccggccatg gggcccgtgg cgaattgccc gaggtacaca cctgcgggcg tgaaccactg
     1741 gactcgatcg ttgccaaagt cccccacata gatcctgttg tccggtcccg tggccagggc
     1801 gttcggatcg agaaactggc cgtcacccga tccgctggtt ccccaccccg tgatcagggt
     1861 tccgtccggg gagaacttcc tgaccatgtt gttggtggca ttcacctggt agacgtttcc
     1921 ggccgaatct actgtcacgt cccttgctcc gagaatggcc ccgcccgtcg caaacgaggc
     1981 gacgaactcg ccttcggccg tgtatcggga gagaaacggc gccggagtgt tgctggaaac
     2041 gtagacatcc ccgaaccggt cgtttgcgat cccgatcgga cccgacccga ctgcccagtt
     2101 gtcgtagaag cgctgggtgt cagccgaagc tgctgccggc aaagcgattg ccagtgcaag
     2161 ggcggccagc aatccggtca gccctgcctt ccgtagaacc gaggtcattt cccctcctct
     2221 tcttgatgtc tgccgtccac cctattcgcg gtggcggtca agttcaggcg aagatagaaa
     2281 aagaggctcc aacctgccgt tgccgcctcc ggcattgatc cctttataaa aaggtgggtg
     2341 cctatcttcg tcccgcgggg gacatcgaaa tcagctcccg tgccgttgat gttgggtcaa
     2401 tttacgagac ggctgtcgaa caggttcgag cctcaacccc aacctatcac tccaggtcaa
     2461 gcccggacac gagttcttcg gcatcggacc acattccggg tggcacctgg aggtggatgt
     2521 ctccccggcc gtagcgatcg agctcccagt cggaggtcag c
//
LOCUS       DAOQFI010000242         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21750_length_2560_cov_2.429940, whole genome shotgun sequence.
ACCESSION   DAOQFI010000242 DAOQFI010000000
VERSION     DAOQFI010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21750_length_2560_cov_2.429940"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /gene="hutU"
                     /locus_tag="PLE93_05125"
     CDS             <1..994
                     /gene="hutU"
                     /locus_tag="PLE93_05125"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNH86466.1"
                     /translation="AAGRALSVGLCANAADVLPELRRIGFPADIVTDQTSAHDPLVGY
                     VPNLMTPDEADALRLSDPDEYVHRSRKAMAAHCAAMVGFLDDGAEVFDYGNSLRAEAR
                     EGGFERAFDYPGFVPAYIRPMFCEGKGPFRWAALSGDPADIAATDRAVLEELPGDESL
                     ARWIRLAGERIAFQGLPSRICWAGYGERHRLGLRFNEMVRTGEVKGPIVIGRDHLDSG
                     SVASPYRETESMADGSDAIADWPLLNAMVNTAAGATWVSIHHGGGVGIGRSIHAGMVC
                     VADGTDLAAQKLERVLTADPGMGVIRHADAGYDRAIEVAGERGVAIPMRDGERG"
     gene            991..1569
                     /gene="def"
                     /locus_tag="PLE93_05130"
     CDS             991..1569
                     /gene="def"
                     /locus_tag="PLE93_05130"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNH86467.1"
                     /translation="MSVREITVIGNPVLREPTRPVDPEELAGPEVQQLIDDLIDTRRA
                     ANGAGLAANQIGSNLRVAVAEVEGVNPRYPYKPPIPRLVMVNPVVEPIGEEMVEINEG
                     CLSVPDLRGNVLRHVEVRVRYLDRDGVEHDEVKRGLTAGTVQHEVDHLDGRLFLDRLT
                     DTASLATWDEFERHQRDEFVERITRFVERVGS"
     gene            1566..>2560
                     /locus_tag="PLE93_05135"
     CDS             1566..>2560
                     /locus_tag="PLE93_05135"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HNH86468.1"
                     /translation="MKLWCEFAWLGGDQADSDVTIGIEAGKIATVEAGTRPPADAERL
                     PGLTVPGFANVHSHAFQRILRGRTHSGHGDFWTWRRQMYAALDGLDPDRYLALATATF
                     AEMAEAGITVVGEFHYLHHAPGGGRYDDPNAMGRAILEAARRAGIRITLLDTCYLHGG
                     IDTTPDEAQIRFSDGDADAWAERVELIDGEVAASHGQENGGIPMARVGAAIHSVRAVD
                     PGSAARVAEWASAHEAPLHAHVSEQPAENEACQAAYGATPSALLEAAGATGPRFTAVH
                     ATHLDEGDFARLGGGGVCLCPTTERDLADGIGPARRLLDAGASISIGTDSNAVIDP"
BASE COUNT          396 a          888 c          902 g          374 t
ORIGIN      
        1 ggcggccggc cgggctctca gcgtcggact ctgcgccaac gcggcggatg tccttccgga
       61 actgcgcagg atcggattcc cggcggacat cgtgaccgac cagaccagcg cccatgaccc
      121 cctggtcggc tatgtcccga acctgatgac cccggatgag gccgacgcgc tgcgcctctc
      181 cgacccggac gagtacgtcc accgctcgcg caaggcgatg gccgcccatt gcgcggccat
      241 ggtcggcttc ctcgacgacg gggccgaggt gttcgactac ggaaacagcc tgcgggccga
      301 agcccgtgag ggcgggttcg agcgggcctt cgactatccc gggttcgtcc cggcctacat
      361 ccgcccgatg ttctgcgaag gcaagggccc cttccgctgg gccgcgctct ccggcgaccc
      421 ggccgacatc gccgcaaccg accgggcggt cctggaggag ctgcccggcg acgagtcgct
      481 ggcccgctgg atccggctgg ccggcgagcg gatcgccttc cagggcctgc catcacggat
      541 ctgctgggcc ggatacggag agcgtcaccg gctcggcctg aggttcaacg agatggtccg
      601 gaccggcgag gtcaagggtc cgatcgtgat cggccgtgat cacctcgact ccggttcggt
      661 cgcttccccc taccgtgaaa ccgaatcgat ggccgacggc tccgatgcga tcgccgactg
      721 gccgctcctg aacgcgatgg tgaacactgc cgcgggggcg acctgggtga gcatccacca
      781 cgggggcgga gtcggcatcg gccgctcgat ccacgccggc atggtctgcg ttgccgatgg
      841 cacggacctg gcggcacaga agctggaacg ggtcctcacc gccgatcccg gcatgggcgt
      901 gattcgccat gcagacgccg gttatgaccg cgccatcgaa gtggccggcg agcgcggggt
      961 agcgatcccg atgcgtgacg gcgagcgggg atgagcgtcc gggagataac cgtgatcggc
     1021 aatccggtcc tccgtgagcc gacccgcccg gtggacccgg aggagctggc cggacccgag
     1081 gtccagcagc tgatcgacga cctgatcgac acccgccggg ccgcgaatgg cgccggcctc
     1141 gcggccaacc agatcggctc gaacctgcgc gtcgcggtgg ccgaagtgga gggagtaaac
     1201 ccgaggtatc cgtacaagcc gccgatcccg cggctggtca tggtgaatcc ggtggtcgag
     1261 ccgatcgggg aggagatggt cgagatcaac gagggttgcc tctcggtgcc ggacctccgc
     1321 ggcaacgtcc tgcgtcacgt cgaggtgagg gtgaggtacc tggaccggga cggggtcgag
     1381 cacgacgagg tcaaaagggg cctgaccgcc ggcaccgtcc agcatgaggt cgaccatctc
     1441 gacggcaggc ttttcctcga ccggctcacc gatacggcca gtctcgccac ctgggacgag
     1501 ttcgaacgcc accagcggga cgagttcgtc gagcggatca cccggttcgt cgagcgggtc
     1561 ggctcttgaa gctctggtgt gaattcgcgt ggctcggcgg cgatcaggcc gactcggatg
     1621 tgacgatcgg gatcgaggcg gggaagatcg cgacggtcga ggccggcacc cggccgccgg
     1681 cggacgccga acgcctgcct ggcctcaccg tgcccggttt cgccaacgtc cactcgcatg
     1741 ctttccagcg gatcctgcgc ggccgcaccc attccggcca cggtgacttc tggacctggc
     1801 ggcggcagat gtacgcggcc cttgacgggc ttgacccgga ccgctacctg gccttggcga
     1861 cggctacctt cgccgagatg gcggaggccg ggatcaccgt ggtcggggag ttccattacc
     1921 tccaccacgc acccggcggc gggcgttacg acgatcccaa cgcgatgggc cgggcgatcc
     1981 tggaggcggc ccggcgggcg gggatcagga tcaccctgct cgatacctgt tacctccacg
     2041 gcggcatcga cacgaccccc gacgaggccc agatccgttt ctcggatggc gacgccgatg
     2101 cctgggcgga gcgggtcgag ctgatcgacg gcgaggtggc agccagccac ggccaggaga
     2161 acggcgggat tccgatggcc cgcgtcggcg ccgcgataca cagcgtccgg gcggtcgacc
     2221 ccggctcggc tgcccgggtc gcggaatggg cctccgccca cgaagctccg ctccatgcgc
     2281 acgtttccga gcagccggcg gaaaacgaag cctgccaggc ggcctacggc gccacgccga
     2341 gcgcgctgct ggaagctgcc ggcgcgaccg ggccccgctt caccgcggtt catgcgaccc
     2401 atctcgacga gggtgacttc gctcgcctcg gtggcggcgg cgtctgcctc tgcccgacca
     2461 ccgagcgtga cctggccgac ggcatcggcc cggcccgccg gctgctcgac gcgggagcct
     2521 cgatctcgat cggcaccgac tccaacgcgg tcatcgatcc
//
LOCUS       DAOQFI010000243         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21798_length_2557_cov_3.232614, whole genome shotgun sequence.
ACCESSION   DAOQFI010000243 DAOQFI010000000
VERSION     DAOQFI010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21798_length_2557_cov_3.232614"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..798
                     /locus_tag="PLE93_05140"
     CDS             142..798
                     /locus_tag="PLE93_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86469.1"
                     /translation="MNEDALPSVSSMDALSRLRRFMAPRKFVSSRRELTEAGFDASRI
                     KNWLRHGRLVLLLRGVYSFGRDVGSRESAWRAALLAAGPGSVLNGRSACELWGMITPT
                     GEIPRYIEVAVTAGEARRLRGTSPALSRTWINIVKRQFSPEDISRVNGLEVTSALVAL
                     VDFAAKASPREVRFAFLEGCRLGLVQKRDVIRLRRLMRGRRGVEKLNPLVALWDEIPG
                     "
     gene            complement(984..>2557)
                     /locus_tag="PLE93_05145"
     CDS             complement(984..>2557)
                     /locus_tag="PLE93_05145"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HNH86470.1"
                     /translation="EGSGDPGKKYDAQFSTKSMWPKFNPDRHYYVTRCVPGRVMVQVR
                     SNPEVKVKVWANPAESGRYAAEARPLPGQDFEVRIMPEGGQAESYEVRCLPSNFPTWR
                     YKRLRKPPKGMFMVSSYLTPGKTGRVWLIVFDQDGTPRWWYSPPYNTLGGQVLYDGTV
                     QFPRGFGDGFGQDARTATDIRDLDGNLVKTVRFDGAPTDGHEYYLLPNGNQLVMSYDP
                     RYGVDLRPIGGRADRGVLDGAFQEQTPDGRVLWSWNSGDHIPLTGTPHRWWIREFNNP
                     HFDRNGKIRYDHFHLNSIEPWGDQYVISTRHTDSIWGISKKTGDIVWKFGGVKTPESL
                     KVVGDDPYGNYPISGNHDARMVGDVLTVHDNGTHVEGRAPRALRYRIDLKNRTATYIG
                     AFTDKAAAPSSHCCGSFRPLGDGWVVSWGDNPWVDGFDSDGQLAFRLGLPVPAYRAVP
                     VPASVTEADLDGGLDAMEPDPPMSNQPMTPLEYNDRHNLEDKTVPSRKLGPIPEDKQQ
                     DLGLTKSGKVAQPDN"
BASE COUNT          398 a          751 c          911 g          497 t
ORIGIN      
        1 atagcgcacc caagtccgat ctcgagctga acgatcaacc tgcctgctct cgcgcccaca
       61 ctccgttccg tgcaccggca cgttgcagaa tcgtcacacc tgtcgaaaca catgtgagct
      121 tttctttgac accgtcgggc aatgaatgag gatgcccttc cgtcggtcag ctcaatggac
      181 gcgctcagcc gcctccgccg cttcatggcc ccccgcaagt tcgtgtccag tcgccgcgag
      241 ctcacggagg ccggattcga cgcctcccga atcaagaact ggcttcgaca cggccgtctg
      301 gtcttgctgc ttcgaggggt ctactcgttt ggtcgagacg tcgggtcgcg ggagtcggcc
      361 tggagggcag ccttgctcgc ggcggggccg ggctcggttc tgaacggccg aagtgcctgc
      421 gaattgtggg gaatgatcac tccgaccggt gagatccccc gctacatcga ggtggcggtg
      481 acggccggcg aagctcgtcg gttgcgagga acttctcctg ctctgagccg gacctggatc
      541 aacatcgtca agcgacagtt cagcccggag gacataagtc gggtgaacgg gcttgaggtc
      601 acctcagccc tcgtggccct ggtcgatttt gcggcgaagg cttccccgag ggaagttcga
      661 ttcgccttcc ttgaagggtg tcggctcggc ctggttcaga agcgggatgt catccggttg
      721 cgacggttga tgcggggccg aaggggcgtg gagaagctga acccgctggt cgccctttgg
      781 gacgagatcc cgggttagat cgaagtcgga gttgggtgcg ctattcggcg caaaaaggga
      841 tctcgcgggg aactgtgctg gaggtcgtga ggcagcccgg ttagttgggt gcgctattcg
      901 gcgccaaacg ggatctcgcg gggattcgtg cccggagacg gatctcgcgg gggttcctgg
      961 cccgaggtcg ggaggttggg tggttagttg tccggttggg ctacttttcc ggacttggtc
     1021 aggccgaggt cttgctgttt gtcttcgggg atcgggccga gcttgcggct ggggacggtc
     1081 ttgtcctcga ggttgtggcg gtcgttgtac tcgaggggtg tcatcggctg gttgctcatc
     1141 ggcgggtcgg gctccatcgc gtcgaggccg ccgtcgaggt cggcttcggt gaccgaggcc
     1201 gggaccggca ccgcgcggta ggccgggacc ggcagaccga ggcggaaggc cagctggccg
     1261 tctgaatcga acccgtcgac ccaggggttg tcaccccagg agacgaccca gccgtctccc
     1321 agcgggcgga acgagccgca gcagtggctg ctcggcgctg ccgccttgtc ggtgaaggcg
     1381 ccgatatagg tcgcggtgcg gttcttgagg tcgatccggt agcggagggc gcgaggggca
     1441 cggccctcga cgtgggtgcc gttgtcgtga acggtcagga cgtcaccgac catgcgggca
     1501 tcgtggttgc cggagatcgg gtagttcccg taggggtcgt caccgaccac cttcagcgac
     1561 tcgggagtct tgacgccgcc gaacttccag acgatgtcgc cggtcttctt gctgatcccc
     1621 cagatcgaat cggtgtgacg ggtcgagatc acgtactgat ctccccaggg ctcgatcgag
     1681 ttgaggtgaa aatggtcgta gcggatcttg ccgttgcgat cgaaatgagg gttgttgaac
     1741 tcgcggatcc accagcggtg gggagtgccg gtgagcggaa tgtggtcacc ggagttccag
     1801 gaccagagca ccctgccgtc cggagtctgc tcctggaagg caccatcgag cacgccgcgg
     1861 tcggcacggc cgccgatcgg ccgcaggtcg accccgtagc gcgggtcgta gctcatcacc
     1921 agctggttgc cgttcggcag caggtagtac tcgtggccgt cggtcggggc gccgtcgaag
     1981 cggacggtct tgaccaggtt cccgtcgagg tcgcgaatgt cggtggcggt gcgggcgtcc
     2041 tggccgaagc cgtcaccgaa cccgcggggg aactggaccg tgccgtcgta gagcacctgg
     2101 ccgccgaggg tgttgtaggg cgggctgtac caccaacggg gggtgccgtc ctggtcgaaa
     2161 acgatcagcc agacccggcc ggtcttgccg ggggtcaggt aggagctgac catgaacatt
     2221 cccttgggcg gcttgcgcag gcgcttgtaa cgccaggtcg ggaagttgga gggcaggcaa
     2281 cggacctcat atgactcggc ctgaccgccc tcgggcatga tcctgacctc gaaatcctgg
     2341 ccgggcaggg gccgggcctc ggccgcgtag cgtcccgact cggcgggatt tgcccagacc
     2401 ttgactttga cttccggatt cgagcggacc tgcaccatga cccgccctgg aacgcagcgg
     2461 gtcacgtagt agtggcggtc gggattgaac ttcggccaca tcgacttggt gctgaactgg
     2521 gcgtcgtact tcttgccggg atccccgctg ccctcgt
//
LOCUS       DAOQFI010000244         2553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21859_length_2553_cov_2.763010, whole genome shotgun sequence.
ACCESSION   DAOQFI010000244 DAOQFI010000000
VERSION     DAOQFI010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21859_length_2553_cov_2.763010"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..850)
                     /locus_tag="PLE93_05150"
     CDS             complement(170..850)
                     /locus_tag="PLE93_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH86471.1"
                     /translation="MDLPEKSDAVLSQPTRARIFELLAESARPVPTTWLADQLGLHPN
                     GVRAHLDRMLQAGLVERRRDAAGRGRPRDEWTLSPDARPGGSPPTAYSDLVRWLARAY
                     PAGTESLRRIEAVGREAGRDAGRKAAPGGSSSPAEALAVTLTAFGFQPRVTGESDEGF
                     TCCLGNCPYREAVHENQPVVCGLHRGLTGGLLETLEPGFRLEGFEPRDPDEAGCVITA
                     IRQTGEPC"
     gene            complement(863..2089)
                     /locus_tag="PLE93_05155"
     CDS             complement(863..2089)
                     /locus_tag="PLE93_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86472.1"
                     /translation="MSSGTMRPTDLLLASVLTGTGFLAAAGALALAGELAGWGTAGWM
                     ALHLALLGGVSQLVIGVSQFFVTAFLATTPPPPKLVAAQTGCWSVGTVAVVTGVAADV
                     DPAVLAGSALLLTTLGLYAAAISGLHRRSLQRAAWASRWYAASAAWLVPGIVAGVLLA
                     TGVAWPYGSLLGAHLAFNLGGWLGGAIVGTLHTFFPSLTRTGLRFGRLQPLTFLLWSA
                     GVGSLAIGYGAGEEPLVLAGWALLLLASGSLSVNLVASAVAAPAGLTLPGRLVASAQV
                     FLPLGVAFALLAALEHPLAPLAGKDQGVLAVLLVVGWIGLTVAGSMLHLISVVVHVRD
                     LGRGGPKKDSWRDRAVSWLAVAGVALLSVARIADLDQLTGPALLLIFAAGLMILSSLL
                     RSLVSAIRLGPGDRFG"
     gene            2156..2419
                     /locus_tag="PLE93_05160"
     CDS             2156..2419
                     /locus_tag="PLE93_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNH86473.1"
                     /translation="MAYVINEPCIAVKDNSCVEVCPVDCIHPTPDEPDYENVDQLFID
                     PEECIDCDACVEACPVDAITPEDMVPPEWQRFIEINANYYKDK"
BASE COUNT          461 a          871 c          895 g          326 t
ORIGIN      
        1 tgtggccgcc ctggtccagc agtcgctcgc gacgatcttc ggggtcgcgc tctaccgcta
       61 cgtggccggg caggaagcgg tcggcggatt caccgagcag gagctcgaga gcgcggtccg
      121 gctcaagcgc ggcgccgggc cagccacgat ctgacccggg ggatccgcct caacaaggtt
      181 cgccggtctg cctgatcgcg gtgatcacgc agccggcctc gtccggatcc ctcggctcga
      241 acccttcgag ccggaatccg ggttcgagag tttccagcag gccgccggtc agtccgcggt
      301 ggagtccgca gacgacgggc tgattctcgt gaacggcctc gcggtagggg cagtttccga
      361 ggcagcaggt gaaaccctcg tcgctttccc cggtgacccg cggctggaag ccgaaggcgg
      421 tgagggttac ggcgagggcc tcggccggtg aggaggagcc acctggcgcg gccttccggc
      481 ccgcgtcccg ccccgcttca cggccgaccg cctcgatcct gcgcaggctt tcggtcccgg
      541 ccgggtaggc ccgggcgagc cacctgacca ggtccgagta ggcggtcggg ggagaaccgc
      601 ccggccgggc atccggcgag agcgtccatt cgtccctcgg gcgtcccctc cccgccgcat
      661 cccggcgccg ttcgaccaga cccgcctgaa gcatccggtc gaggtgggcc cggacgccat
      721 tcgggtggag gcccagctgg tcggcaagcc aggttgtcgg caccggccgg gcggactccg
      781 caagcagctc gaagatccgg gcccgggtcg gctggctcag cacggcatcg ctcttttccg
      841 gcaggtccac ggactcagcc tctcacccga agcggtctcc gggcccgagc cggatcgcgc
      901 tgaccaggct tcgcagcagg ctcgagagaa tcatcagccc ggcggcaaag atcagcagca
      961 gggccggtcc ggtcagctgg tccaggtccg cgatccgggc aaccgaaagc agcgccaccc
     1021 cggccacggc cagccagctg accgctcgat cccgccacga gtcctttttc ggcccgcccc
     1081 gcccgaggtc tctcacatgc acgaccaccg agatcaggtg aagcattgaa ccggcgacgg
     1141 tgaggccgat ccatccgacc acgagcagca ccgcgaggac gccctgatcc ttgcccgcca
     1201 gcggggccag gggatgctcg agtgcggcca gcagggcgaa ggcgacgccg agcggcagga
     1261 agacctgggc ggaggcgacc agccggcccg gcagggtcag cccggccggg gcggcgaccg
     1321 ccgaggcgac gagattcacg gaaagcgagc ccgaagccag taacagcagc gcccagccgg
     1381 caaggaccag tggttcttct ccggcgccgt aaccgatcgc cagcgatccg acgccggcgc
     1441 tccagagcag gaaggtcagg ggctgaaggc ggccgaaacg aagcccggtc cgggtcaggg
     1501 acgggaagaa ggtgtggagg gtgccgacga tcgcaccacc cagccagccg cccaggttga
     1561 aagcgagatg ggcgccgagc aaggagccgt agggccaggc gacgccggtc gcgagcagga
     1621 cgccagccac gatgccggga accagccagg cggcgctcgc ggcgtaccat cggctggccc
     1681 atgcggcccg ctggagggag cggcgatgca gcccggaaat ggccgccgcg tagagcccga
     1741 gggtggtcag gaggagtgcg gaaccggcga gcacggccgg gtcgacgtcc gcggcaaccc
     1801 cggtcacgac cgcaacggta ccgacgctcc agcaaccggt ctgggccgcg accagcttcg
     1861 gcggaggcgg tgtggtggcg aggaaggcgg tcacgaagaa ctggctgacc ccgatcacga
     1921 gctgggacac cccgccgagc agggccaggt gaagtgccat ccagccggcg gttccccagc
     1981 cggcaagctc accggccagc gcgagggctc ccgcagcggc cagaaacccg gttccggtga
     2041 ggacggaagc aagcagcaga tcggtgggcc gcatggttcc ggaggacatg acgcgatttt
     2101 acacacaatg actgtgtaaa atggtcgccc cgaatccgaa tcgaacgagc gtgagatggc
     2161 ctacgtaatc aacgaaccct gcatcgccgt gaaggacaac tcctgcgtcg aggtctgtcc
     2221 ggtcgactgc attcatccga ctcccgacga gcccgactac gaaaacgtgg accagctctt
     2281 catcgacccg gaggagtgca tcgactgcga cgcctgtgtc gaggcctgcc cggtcgacgc
     2341 gatcaccccg gaggacatgg tcccgccgga gtggcagcgc ttcatcgaga tcaacgcgaa
     2401 ctactacaag gacaagtagt ccggcgaacc tcgcctcgcc gccgggccgg accggcccgg
     2461 tcacgacctc ggttcaattc acttggacat aacccgggcg aattgaacct aggtggcctg
     2521 attgaggagg cgggggtcgt tcgagcagat ccc
//
LOCUS       DAOQFI010000245         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_21963_length_2546_cov_2.810518, whole genome shotgun sequence.
ACCESSION   DAOQFI010000245 DAOQFI010000000
VERSION     DAOQFI010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21963_length_2546_cov_2.810518"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1849
                     /locus_tag="PLE93_05165"
     CDS             <1..1849
                     /locus_tag="PLE93_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNH86474.1"
                     /translation="RGTPALIFWLAAATLALIVVFTIVFLVFGLSPEGGDPHKGVIGE
                     AYFTLLHALDPGTITGDDGDWVFLFLMFLVTLGGLLIFSALIGVLSTGLDARLQELRK
                     GRSLVLEEGHTLILGWGKPVFTILAELDIASEGEADPVVVVLSETDKVVMDDTLRERS
                     DLKNLRVVTRHGSPIDVDDLALARPELASATIVLAPLEEPEPDAYVIKVILALTQLLQ
                     GREVERGIVAEISDPANLSPARLAGAGQAVLLDGRETIAKLLVQAARQSGISVAVQEL
                     LDFDGHEIYIDDASRFEGIDFGTLLGRLERCSVIGLLDVDDMVLLNPPGSKVISSGDR
                     LIAVAEDDTVLELFEESVSGPDFEQILAADERSPSPERVVILGWNKGAQSVITHFDAF
                     LAPGSRIEVVVDPGLGRAPEVDSHQRLANTDVNFRHGSTTDRSTLEELELGQADHVMV
                     LACSDDFDAQRADARTLVTLLHLREINDGLEPGFSIVSEMVDERSRRLAEVAEVDDVI
                     VSENVISLLFAQISQNHHLADVFTQLFSPEGSEIYLRPAGTYVALNEEVSFATMVEAA
                     RRRGECAIGYRSASQSDDPDSGFGMTINPPKSRVFKVTQGDSLVVLAEE"
     gene            complement(2066..>2546)
                     /locus_tag="PLE93_05170"
     CDS             complement(2066..>2546)
                     /locus_tag="PLE93_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HNH86475.1"
                     /translation="LVLLLYRSSRPHVTRLGELRAQPGHWGDLQRHANAVEVPQIVVL
                     RLEAAIYFANSDEIRQAVIRSLAADTRAVVLDVETVPYIDVTAAYMLKALREELDQRQ
                     VRLVIARDVGQVEDMMRSTGLEVLLTDSYVSIDQAVDSVTAGIRSTSGDRPSGAATT"
BASE COUNT          429 a          840 c          822 g          455 t
ORIGIN      
        1 gaggggtacc ccggccctga tcttctggct ggccgcggcg accctggcgc tgatcgtcgt
       61 cttcacgatc gtcttcctgg tcttcggtct ttcacccgag ggcggggatc cgcacaaggg
      121 tgtgatcggc gaggcctact tcaccctgct tcacgcgctc gatccaggaa cgatcaccgg
      181 cgacgacggc gactgggtct tcctcttcct catgttcctg gtcacgctcg gcggcctcct
      241 gatcttcagc gccctgatcg gcgtgctctc gaccggtctc gatgcccgtc tgcaggaact
      301 tcgcaaggga aggtcactgg tcctcgaaga gggtcacacg ctgatcctcg gctggggcaa
      361 gccggtcttc acgatcctcg ccgaacttga catcgccagc gaaggggaag cggacccggt
      421 ggtggtcgtg ctttccgaga ccgacaaggt ggtgatggat gacaccctgc gggagcggtc
      481 cgacctgaag aacctgcgag tggtcacccg gcacggcagc ccgatcgatg tcgacgacct
      541 ggcgctggcc cggcccgagc tggccagtgc cacgatcgtg ctggcgccgc tggaggaacc
      601 ggagcccgac gcctacgtga tcaaggtgat cctcgccctt acccagctcc ttcagggccg
      661 cgaggtcgag cggggcatcg tcgccgagat ctccgacccg gccaatctct ccccggcgcg
      721 gctggccgga gccgggcagg ccgtgctgct ggacggccgg gagacgatcg ccaagctgct
      781 ggtgcaggcg gcccgtcagt ccggaatctc ggtcgccgtc caggagttgc tcgatttcga
      841 tggccacgag atctacatcg acgatgcctc gcgcttcgag gggattgact tcggcaccct
      901 gctcggccgg ctggaaaggt gttcggtgat cggcctgctg gacgtggatg acatggttct
      961 gctgaacccg cccggctcga aggtgatctc gtccggagac aggctgatcg ccgtggccga
     1021 agacgacacg gtgctcgagc ttttcgagga atcggtctcc ggtcccgact tcgagcagat
     1081 cctggcggcc gacgaacgga gcccttcgcc ggaaagggtc gtgatcctcg gctggaacaa
     1141 gggggcgcag tcggtcatca cccacttcga cgccttcctc gcgcccggat cacggatcga
     1201 ggtggttgtc gatcccggcc tgggccgggc accggaagtc gacagccacc agcgcctcgc
     1261 caataccgac gtgaatttcc gacatggttc gaccaccgac cgctccaccc tggaggaact
     1321 cgagcttggg caggccgacc acgtgatggt gctggcctgc tcggatgact tcgatgccca
     1381 gcgcgccgac gcccgcaccc tggtcaccct gctccacctg agggagatca acgacggtct
     1441 tgagcctggc ttctcgatcg tcagcgagat ggtggacgag cgaagccgcc ggctggccga
     1501 ggtggccgag gtcgatgacg tgatcgtcag cgagaacgtg atcagcctgc tcttcgcaca
     1561 gatctcgcag aaccaccatc tggccgatgt cttcacccag ctcttctctc cggaggggag
     1621 cgagatctac ctgcggccgg ccgggaccta cgtagcgctg aacgaagagg tcagtttcgc
     1681 gaccatggtc gaggcggccc gccgccgcgg ggaatgcgcg atcgggtatc gctcggcttc
     1741 acagtccgat gacccggact cgggtttcgg catgacgatc aacccgccca agtcccgggt
     1801 cttcaaggtc acacaaggcg acagcctcgt cgtcctcgca gaggaatgag acttctgttc
     1861 ggagcagagg gccggccggg tcgttgaatg caaggcggcg aggcctgggg cctcgccacc
     1921 tccccctgct tctgtcagtc cctgccgtgc atccctccct ggcacgcgga ctgcccctat
     1981 aaccgggcag gtggccaagg gttgcggtga aattcgaacc ggacaaaaaa ctttccgaaa
     2041 ggtgttcggc accgcggcga accggtcagg ttgtggcggc gcctgacggc ctgtcgccgg
     2101 aggttgagcg aatgcccgcg gtcaccgaat caaccgcctg gtcgatcgag acataggagt
     2161 cggtcagcag cacctcgagg ccggtcgatc tcatcatgtc ctcgacctgc ccgacgtcgc
     2221 gggcgatgac gagccggacc tgcctttgat cgagttcctc ccgcagcgcc ttcaacatgt
     2281 aggcggcggt cacgtcgatg tagggcaccg tctccacgtc cagaaccacc gcccgggtgt
     2341 cggccgcaag gcttcgaatc accgcctggc gaatctcgtc tgaattggcg aaatagatcg
     2401 ccgcttcgag tctcagcacc acgatctgcg ggacctcgac cgcgtttgcg tgccgctgca
     2461 ggtcacccca gtgtccgggc tgcgccctga gctcaccgag cctggtcaca tgcggccgcg
     2521 aggagcggta aagcagcagc accagc
//
LOCUS       DAOQFI010000246         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22046_length_2541_cov_3.105390, whole genome shotgun sequence.
ACCESSION   DAOQFI010000246 DAOQFI010000000
VERSION     DAOQFI010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22046_length_2541_cov_3.105390"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLE93_05175"
     CDS             complement(<1..276)
                     /locus_tag="PLE93_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH86476.1"
                     /translation="MRVVVADDSILLREGVVRVLEDDGFEVVGQAGDAEDLLRKVSAH
                     KPDVAVVDIRMPPNLTDDGLQAAKKIRAEHPDTGVLVLSQYLEESYAL"
     gene            complement(267..2024)
                     /locus_tag="PLE93_05180"
     CDS             complement(267..2024)
                     /locus_tag="PLE93_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH86477.1"
                     /translation="MGSALNLRARLTWLAIAGFLAGAGGATMIALTNQTLGEPVIEIT
                     LRVLTGWTWMGVGIFAWWRRPDNPFGKWMVFTSFAWLAQALTYANSSLVFTVGLVIAY
                     VFPFALIQTLVRFPTGRIETRADGWIVRLVAGALLLVLLATFFSSPDQLGYDNAPSNL
                     LLLADSPTTSAVFFIIANAVAIILVLGLIHSFTTKYSAASPPMRRLIGPVLWTSTTLL
                     AVLGLSSVFSIANLDTVEEIAGILGAIIAMLLPLAFLVGLARSRLVRGEALGRMLERV
                     AHESDPVKMQEAVAEALGDPSVEIAFWLPDTAGYVDARGLPIAEPDPDSNRAASQVTM
                     DGRRVALIIHDGALLEEPEKIEAVSRGAALAFENARLEAEVRADVAELRASRTRIVEA
                     GDAARREIERNLHDGAQQNLVSLALKLQLARSRVESDPRGAIEIIDGASAELEQTLAE
                     LRELARGIHPAVLSDRGLDAALLALATRAPLPVEVGEMPDDRLPEPVEAAAYYVVAES
                     LTNVARYSEASRAKVDITRENGVVRIRIADDGVGGADPARGSGLKGLADRIAALDGHF
                     MLASPDGLGTIVEAEIPCG"
     gene            2188..>2541
                     /locus_tag="PLE93_05185"
     CDS             2188..>2541
                     /locus_tag="PLE93_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86478.1"
                     /translation="MDTSRNIAARAGRWSATHRKTAIWGWLAFVILAVFIGGSVGTRT
                     LTDAEDGVGESGRAEVATSNNFPENATERVLITAPAASIWFRCSVIVLPSGARGTICS
                     SCSDGCVSATDTSRSV"
BASE COUNT          428 a          826 c          898 g          389 t
ORIGIN      
        1 gagggcgtag ctctcctcca gatactggga aagcacgagg acaccggtgt ccgggtgttc
       61 ggcccggatc ttcttggccg cctgaagccc gtcgtcggtc aggttcggcg gcatgcggat
      121 gtcgaccacc gcgacatcgg gcttgtgggc actgaccttg cggagaaggt cttcggcgtc
      181 acccgcctgg ccaaccacct cgaaaccgtc gtcctcgagc acgcggacca ctccttcgcg
      241 cagcaggatc gagtcgtcgg cgacaactac ccgcacggga tctccgcctc cacgatcgtg
      301 cccagaccgt ccgggctggc cagcatgaag tgcccgtcca gggcggcgat ccggtcggcc
      361 aggcccttca gcccggaccc ccgggcgggg tcggctccgc cgacgccgtc gtcggcgatc
      421 cggatgcgga ccaccccgtt ttcccgggtg atgtcgacct tggcccggct tgcctcggaa
      481 tagcgggcga cgttggtcag cgactcggcg accacgtaat aggcggcggc ctcgaccggt
      541 tccggcaggc gatcatccgg catttctccg acctcgaccg gcagcggagc gcgagtcgcg
      601 agggcgagca gggcggcatc caggccgcgg tcggaaagca cggcggggtg gatgccacgg
      661 gccagttcgc gaagctcggc cagggtctgt tcgagttcgg cgctggcgcc gtcgatgatc
      721 tcgatcgcgc cgcgggggtc ggactcgacc cgggaacggg ccaactgcag tttgagggcg
      781 agcgaaacca ggttctgctg ggcgccgtcg tggaggttcc gttctatttc gcggcgggcg
      841 gcgtccccgg cctcgacgat gcgggtgcgg gaagccctca gttcggccac gtcggcccgg
      901 acctcggcct ccagccgcgc gttctcgaaa gccagtgcgg cgccacggct caccgcctcg
      961 atcttctccg gttcctcgag cagggcgcca tcgtggatga tcagcgcgac ccttctgccg
     1021 tccatcgtga cctggctcgc cgcccggttc gaatccggat caggctcggc gatcggcagg
     1081 ccacgggcgt cgacgtagcc ggcggtgtcc ggcagccaga aggcgatctc gaccgagggg
     1141 tcgccgagcg cttcggcgac cgcttcctgc atcttcaccg gatcgctttc gtgggccacc
     1201 cgttcgagca tccggccgag cgcctcgccg cggaccagcc ggctgcgggc caggccgacc
     1261 aggaaggcga gtggcagcag catcgcgatg atcgcaccga ggatgccggc gatctcttcg
     1321 accgtatcca gattggcgat cgagaagacg gaggaaagcc cgagcacggc cagcagggtg
     1381 gtggaggtcc agaggaccgg tccgatcagg cggcgcatcg gaggcgaggc ggccgaatac
     1441 ttggtcgtga agctgtggat cagaccgagg acgaggatga tcgccaccgc gttggcgatg
     1501 atgaagaaga cggccgaagt ggtcggcgag tcggccagca gcagcaggtt gctcggggca
     1561 ttgtcgtatc cgagctgatc gggagagctg aagaaggtcg ccagcagaac aagcaggagg
     1621 gcgccggcaa ccaggcggac gatccacccg tcggcccggg tttcgatccg gccggtcggg
     1681 aagcggacca gggtctggat cagggcgaag ggaaagacat aggcgatgac gaggccgacg
     1741 gtgaagacca gcgacgagtt cgcgtaggtc agggcctggg ccagccaggc gaaactggtg
     1801 aagaccatcc acttgccgaa cggattgtcg gggcggcgcc accaggcgaa gatgccgact
     1861 cccatccagg tccatccggt cagcacccgc agcgtgatct cgattaccgg ctcgcccagg
     1921 gtctggtttg tcagggcgat catcgtcgcc ccgcccgctc cggccaggaa gccggcgatc
     1981 gccagccagg tcagcctcgc ccgcaggttc agagcactac ccatggggtg attctgaccc
     2041 atgcggggac cgccgttggt gcggccagta cccgcatccg ggcgttcatg gtgcggtttg
     2101 ccgaacccct aaggggcagg catcacccgc tgaagacaca gggaggcccc attttgcggc
     2161 ggcgtccgca aaggcaggct tggcggcatg gataccagtc gaaacatcgc agcccgtgcc
     2221 ggaaggtgga gcgcgaccca tcgcaagacc gcgatctggg gttggctcgc cttcgtcatc
     2281 ctggccgtct tcatcggagg atcggttgga acgaggaccc tgaccgacgc cgaagacgga
     2341 gttggcgagt cggggcgcgc cgaagtggcg acctcgaaca acttccccga gaacgcgacc
     2401 gaaagggtgc tgatcacggc gccggccgcg tcgatctggt tccgctgcag cgtgatcgtg
     2461 ctgccgtccg gcgcccgcgg cacgatctgc agctcctgca gcgacggatg cgtcagcgcc
     2521 accgacacgt cgaggtcggt g
//
LOCUS       DAOQFI010000247         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22198_length_2532_cov_2.816714, whole genome shotgun sequence.
ACCESSION   DAOQFI010000247 DAOQFI010000000
VERSION     DAOQFI010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22198_length_2532_cov_2.816714"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLE93_05190"
     CDS             <1..669
                     /locus_tag="PLE93_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNH86479.1"
                     /translation="PLEAKALVAAIDLVGDHLPQARLDSAREKIVEALGRDPAREGLA
                     IVRESGGAEAAGIVNEAIAKRQVLALNYYKENEDKFSERKVEPYRLENGREGWYAECF
                     DLDRMAMRHFKLDRIKEATPTGETFEPRPEAVELAGVEGWMSDGQVPEASVARVWVSP
                     ERSRWMREERNVVEELADGAVVVELPYAGTSWLVREILKGAGDLVVLEPAEAREAISR
                     ELAA"
     gene            704..1492
                     /locus_tag="PLE93_05195"
     CDS             704..1492
                     /locus_tag="PLE93_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH86480.1"
                     /translation="MSGEMVNGAAPGFPEIWRVTADNPGPMTLDGTNTYLYGSNPCWV
                     IDPGPADQGHQAIIKEAIEDRGGAAGVLLTHSHVDHAGGAEMLGLPVVEAGKVTGSFR
                     DGMVAADLESGSEIFSIDTPGHSSDHVCFMATPIPGGACFSGDLILGRGFTYVPPDGG
                     SLASYMDSLKLLQTWQPALICPGHGPWITDPAAKISEYLEHRLMRENGLVEAMAAGER
                     SREALLDLVWSDVPEQLKSAARMVMEAHLQKLKAEGRLPDGLVK"
     gene            1517..>2532
                     /locus_tag="PLE93_05200"
     CDS             1517..>2532
                     /locus_tag="PLE93_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86481.1"
                     /translation="MKESAPVELPKNLQLKELLRTIVGFGIPFLLIAYLAFASGGYEI
                     VPRSQVGIIAWWSVLLGILAGILPLFRPTRASLAILAILCALLLWTACGLLWTESTEH
                     TIIELTRVATLLGVVVLLVVTQDREGLRNAVVAVAAAVVLVALVALVDRLLPNLLPFG
                     SDYTFPEGYPKARLNYPLEYWNGLGIFIAIGVGPVLWLVAAAKSTAVRCLAAGAVPLL
                     ALAGYMTASRGEIVEVAAVLAVLAVLFPERTRLLLNLIGPAIGSALLIFLINDRPAVR
                     DLDPGQARLDQGEQMALLVLAVVSAVALAQFAAFFFANRNEIKLGRPSRNAARNAGIA
                     AFAVV"
BASE COUNT          477 a          739 c          806 g          510 t
ORIGIN      
        1 ccgctcgaag ccaaggcgct ggtcgccgcg atcgacctgg tcggcgacca cctgccccag
       61 gcccggctcg actcggcccg ggagaagatc gtcgaggccc tcggccgcga cccggcccgt
      121 gaaggcctcg ccatcgtgcg ggaatccggt ggagccgagg cggccggaat cgtcaacgag
      181 gcgatcgcca agcgccaggt cctggccctc aattactaca aggagaacga agacaagttc
      241 tccgagcgca aggtcgagcc ctaccggctg gagaacggtc gcgagggctg gtacgccgag
      301 tgcttcgacc ttgaccgcat ggcgatgcgc cacttcaagc tcgaccggat caaggaagct
      361 acgccgaccg gtgaaacctt cgagccacgg cccgaggcgg tagagctggc cggggtggaa
      421 ggctggatga gcgacggcca ggtccccgag gcctcggtcg cccgggtctg ggtctcaccc
      481 gagcgctccc gctggatgcg cgaggagcgc aacgtggtcg aagaactcgc tgacggcgcc
      541 gtcgtggtcg agctccccta cgccggcaca tcatggctgg tccgcgagat cctcaaggga
      601 gcaggcgatc tagtcgttct cgagcccgca gaggctcgcg aagcgatcag ccgcgaacta
      661 gccgcctgat cagaggggct tcttggtgat ctaggctctt cccatgagcg gggaaatggt
      721 gaatggtgcg gcgccaggat ttccggaaat ctggagggtg acagccgaca atccgggacc
      781 gatgaccctg gatgggacca acacctatct ctacggctcc aacccgtgtt gggtgattga
      841 tccaggtccg gcagatcaag ggcatcaggc aatcatcaag gaagctatcg aggaccgagg
      901 tggggcggcg ggggtgctgc tcacgcattc gcatgtcgat cacgcgggag gagccgagat
      961 gttggggctg cccgttgtcg aagccgggaa agtcacgggg tctttcagag acgggatggt
     1021 cgcggccgac ctcgaatcgg gatctgaaat ctttagcatc gacactcccg gccactcttc
     1081 cgatcacgtt tgcttcatgg ccaccccgat ccccggcgga gcctgcttca gcggggatct
     1141 gatcctcggc cgaggattca cgtatgtccc ccccgacggc ggttccctgg cttcgtacat
     1201 ggattctcta aagcttctgc agacttggca accagctctg atctgtcccg gacacggacc
     1261 ctggattacc gatccggcgg cgaaaatatc cgaatacctt gagcatcggt taatgagaga
     1321 gaacggactt gtcgaggcaa tggccgctgg cgaaaggtca agagaagcgc tgctagatct
     1381 cgtctggtcg gatgtgcctg aacaattgaa gtcagcagcg agaatggtta tggaggcgca
     1441 tcttcagaaa ttgaaagcgg agggccgact gcccgatggc ctcgtgaaat agccagcgac
     1501 ggacgagcta cctcagatga aagaatccgc gcctgtggaa ttgcccaaga accttcagct
     1561 caaagaactg cttcgaacaa tcgtaggctt cgggattcct ttcctgttga tagcctacct
     1621 ggctttcgct tctggcggct acgaaattgt gccgcgaagt caggtcggga tcatagcttg
     1681 gtggtcagtg ttgcttggga ttctggcggg gattctgccc ctatttcgcc cgacccgggc
     1741 gagtctggcc attctggcaa ttctgtgtgc cctcctgctg tggaccgcct gcggtctgct
     1801 ctggactgaa agtaccgaac acaccatcat tgaactgacc cgggttgcca cattgctcgg
     1861 agttgtggtt ctactggtag ttacccagga tcgtgaggga ctgagaaacg cagtagtagc
     1921 tgttgcggca gccgtggttc tggttgcttt ggtcgccttg gtcgatcggc tgctgccgaa
     1981 cctgctcccg ttcggctccg actacacctt cccagaggga tacccgaagg cccggctcaa
     2041 ctatccgctc gaatactgga acggattggg gattttcatt gccattggcg tcggacccgt
     2101 gctctggctc gttgcggcag caaagtcgac ggcggtgcga tgtctggccg caggtgccgt
     2161 tccgctgctg gccctcgccg gttatatgac cgcatcgcgt ggcgaaatag tcgaggttgc
     2221 agcggttctc gccgttctgg cggttctctt tcccgaacga acaagactgc tcttgaacct
     2281 gatcggcccg gcaatcggtt cggccttgct catctttctg atcaacgaca ggccggcggt
     2341 ccgtgatctc gacccggggc aggcccgcct agatcagggc gagcagatgg cgttgctggt
     2401 cctggcggtg gtttcagcgg tcgcgttggc tcagtttgcc gcatttttct ttgccaacag
     2461 gaacgagatc aaactaggcc gaccaagtcg gaacgctgca cgtaacgcgg ggatcgccgc
     2521 ctttgcggtg gt
//
LOCUS       DAOQFI010000248         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22260_length_2526_cov_4.141643, whole genome shotgun sequence.
ACCESSION   DAOQFI010000248 DAOQFI010000000
VERSION     DAOQFI010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22260_length_2526_cov_4.141643"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..666)
                     /locus_tag="PLE93_05205"
     CDS             complement(49..666)
                     /locus_tag="PLE93_05205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNH86482.1"
                     /translation="MSDRLKIVSVLGSVTAPGRMHRALNEALDRAGTGAGGATGDGEV
                     AVERIDLAEKTVTFADGTPLDQLGDDSAAVIGQIARADAVVLATPVYRGSMTGALKNL
                     LDLTPVEALRGKPVLIVAMGATDHHSLGVERHLRDVLSFFGALPVPVDVYLTSADFTD
                     GVPGDDAARRLDQGLDGLLALTRATGGLGEFGPDPLITRLSRPQS"
     gene            complement(688..1305)
                     /locus_tag="PLE93_05210"
     CDS             complement(688..1305)
                     /locus_tag="PLE93_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86483.1"
                     /translation="MTPEIVDRAEWGPKERQIYAYLLGQYLGDGYIGTHGSTHSLVIA
                     CDIQYCRILTQVVEAVGTFAPKPPSVLRPKGTNGVRVTSYWRSWPQLFPQHGPGSKLD
                     RKIELVDWQVEIVDEHPKAFLRGLIHSDGSRCMNTFKMALKDGPKEYSYPRYFFTNYS
                     ADIQAIFCRTCDRLGIRWSRSNWRNISISHRTSVALLDEFVGPKD"
     gene            1546..1618
                     /locus_tag="PLE93_05215"
     tRNA            1546..1618
                     /locus_tag="PLE93_05215"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1579..1581,aa:Trp,seq:cca)
     gene            1689..1759
                     /locus_tag="PLE93_05220"
     tRNA            1689..1759
                     /locus_tag="PLE93_05220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1721..1723,aa:Gly,seq:tcc)
     gene            1832..1905
                     /locus_tag="PLE93_05225"
     tRNA            1832..1905
                     /locus_tag="PLE93_05225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1866..1868,aa:Pro,seq:ggg)
     gene            complement(2000..>2526)
                     /locus_tag="PLE93_05230"
     CDS             complement(2000..>2526)
                     /locus_tag="PLE93_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNH86484.1"
                     /translation="LTKATLLDSGAFVQVGGWGVPLFEAEVAVRIDSEIGAGATAGEA
                     TAAAGAVAAAIELVDFGSIESVEEILAGNIFHRHVLLGEFVELPPDGLDGVSISVAAN
                     GEAAEASDPREALGDLGQVVAALADQAALTGESIKPGDVVITGAAIPPAPVSPGDDYG
                     VEITGGSSVTVKLV"
BASE COUNT          439 a          853 c          779 g          455 t
ORIGIN      
        1 ctgcagcccg cccgccaggg tcgcggcgac ctggggttcg cgacctgctc agctctgcgg
       61 ccgcgagagc cgggtgatca gcgggtcggg gccgaactcg ccgaggccgc cggtcgcccg
      121 ggtcagcgcc agcaacccat cgaggccctg gtcgagtcgc cgggctgcgt catcgcccgg
      181 cactccgtcc gtgaaatcgg ccgaggtcag gtagacgtcg acaggaactg gcagcgcacc
      241 gaagaagctg agtacgtccc gcaggtgccg ttcgaccccg agcgagtgat ggtcggtcgc
      301 tcccatcgcc acgatcagaa ccggcttgcc ccgcaacgcc tcgaccgggg tcaggtcgag
      361 caggttcttg agcgccccgg tcatcgagcc ccggtagacg ggcgtggcga gcaccacggc
      421 gtcggcccgg gcgatctgcc cgatcacggc ggccgagtca tcacccagct ggtcgagcgg
      481 ggtgccgtcg gcgaaggtga ccgtcttctc ggccaaatca atccgctcaa cagcgacctc
      541 accgtcgccc gtcgcaccgc cagcacccgt cccggcccgg tcgagcgcct cgttcaaggc
      601 ccggtgcatc cgccccggag ccgtgacgct ccccagcacg gaaacgattt tgagtcggtc
      661 gctcatctca gttcctcagc ataggcgtca atccttgggt cctacaaatt catcgagcag
      721 cgcgacgctg gtccggtgcg agatcgagat gttccgccag ttcgaccggc tccaacggat
      781 ccccagccgg tcacaggtcc ggcagaagat cgcctggatg tcggccgagt agttggtgaa
      841 gaagtagcgg gggtacgagt actccttcgg cccgtccttc aaggccatct tgaaggtgtt
      901 catgcaccgc gacccatccg aatgaatcag ccccctaagg aaagccttcg gatgctcgtc
      961 cacaatctcc acctgccaat ccaccagctc aatcttccga tccagcttgc tccccggccc
     1021 atgctgagga aagagctgag gccagctgcg ccaataggaa gtgaccctta cgccgttggt
     1081 gcctttgggg cgcagcactg acggcggctt cggggcgaac gtgccgacag cctcgaccac
     1141 ttgggtgagt attcggcagt actggatgtc gcaagcgatc acgagtgagt gggttgaccc
     1201 gtgggttccg atgtaaccgt ctcccaggta ctggccgagc aagtaagcgt agatctggcg
     1261 ttccttgggc ccccactcgg cccgatccac tatctccggc gtcatgggca cccgcggaga
     1321 tggaacgcca tggtgcctcc acctaaggac agtggatctt ccgacaccgg tcctcttcgc
     1381 gatctcgaaa tccgaaacgc ccgagtcgat cagcgacctg acttcttctc gagttgcttc
     1441 cgaatggatc cggtacacga acatatgttc gcaccgggat cggatggaat gagcgttttt
     1501 cgggggtgac tcctacgcct ttgaggcgct aggatcagat cctccggggc actagctcaa
     1561 ttggctgagc agcggcctcc aaagccgaag gttccaggtt cgagtcctgg gtgccccgct
     1621 tcccgggtcg attttcgacg gatcggagtc aggattcggc ccctataatt ggtggtcgcg
     1681 atttctctgc gggcgaggtg tattggttgc acaggagcct tccaagctcc tagggcgggt
     1741 tcgattcccg tcgcccgctt cacccggtcc cggggcgcat gctccgtaca gcggcgccaa
     1801 gccgtctccc gctaaagtcc ccggtcacgc acggggcgtg gcgcagtttg gtagcgcatt
     1861 cgactggggg tcgaaaggtc gctggttcaa atccagtcgc cccgattttc aggctgaccc
     1921 gtgaggtccg gacttctggg ttgaagttat ccgcacatgg aacgaataac ttcaatgcag
     1981 ttggttccca cgcgttcgtt cacaccagtt tgaccgtgac cgagctgccg ccggtgatct
     2041 cgaccccgta atcgtccccc gggctgaccg gggcgggagg gatggcggca ccggtgatca
     2101 ccacgtctcc cggcttgatc gactccccgg tcagggcggc ctggtcggcc agtgcggcca
     2161 ccacctgccc gagatcaccg agggcctccc gggggtcgga ggcctccgcc gcctctccgt
     2221 tggcggccac cgaaatgctg accccgtcga gcccgtccgg agggagctcg acgaactcac
     2281 caagcaacac atgacgatgg aagatgttcc cggcgaggat ctcctcgacg ctctcgatcg
     2341 atccgaagtc gaccagctcg atcgcggccg ccaccgcacc ggccgcggcc gtcgcctctc
     2401 cggcagtcgc gccggcaccg atttccgaat cgatccggac cgcgacctcg gcctcgaaca
     2461 aaggcacgcc ccatccgccg acctggacga atgcccccga gtcgagcagc gtcgccttcg
     2521 tcagga
//
LOCUS       DAOQFI010000249         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22317_length_2523_cov_2.894246, whole genome shotgun sequence.
ACCESSION   DAOQFI010000249 DAOQFI010000000
VERSION     DAOQFI010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22317_length_2523_cov_2.894246"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLE93_05235"
     CDS             <1..650
                     /locus_tag="PLE93_05235"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNH86485.1"
                     /translation="GTRTATLPGATVEMLSRVMSDDMEKLRHRGHRVAELLDRGTEAR
                     LTCDHGSNLKLGLQGRVAIPDAGELTETGAFGNLPCGEGFMAPATAEGVLVVDGSIAG
                     VGLVDEPVRLTIKDGHLVDATGEAGAKLMELLTVHGPDGTNVAELGIGTNEKAILTGN
                     ILEDEKILGTAHIAFGASAAIGGNVQVPVHLDVVSMRPEVTIDGTPVVLEGRLLV"
     gene            647..1546
                     /locus_tag="PLE93_05240"
     CDS             647..1546
                     /locus_tag="PLE93_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86486.1"
                     /translation="MSLLAVPNVSEGRDFARLDRLEAALGQEVTLLDRHTDLDHHRAV
                     FTLAGPAETLADALTGLAGTATVEIDMTAWDGMHPAIGALDVCPVVWLSERRRQMASE
                     VALEVGEKIGAMGVPVFLYGDLASDADRRERAYFRAGGLDSLWQRMTSGALRPDFGPN
                     QPHPTAGATLVTARAPIAAFNLELATDDLAVAREIAAAVREGPDDGIPGVRALGLLLS
                     TGRAQVSTNVHDPIETPLAEIVDAVQEEATLRGTYVVEAELIGLIPEAALEEYPEGVP
                     IRDFDPAFHVIERRLKPRDQAGQ"
     gene            1570..1944
                     /locus_tag="PLE93_05245"
     CDS             1570..1944
                     /locus_tag="PLE93_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86487.1"
                     /translation="MAQTKKKRSRKKRGTQAGNLDTSRRSRPRSRAEARNQARSGGGK
                     GGRTMQKGDAPPTWRGATIRGLIAAGVFVLLLLLLFGRPLATSLIIGVVMLIFYIPAG
                     YYMDLYLWRRRERQRIRAKSKG"
     gene            1954..>2523
                     /locus_tag="PLE93_05250"
     CDS             1954..>2523
                     /locus_tag="PLE93_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH86488.1"
                     /translation="MAGLEVIGTTVGPVAENSYLVFKEETGKALLFDPGDEADRLMQP
                     LADRGLELEAILLTHCHFDHIGAVAPIARATGAPVYCPLIEVPILADVMAYVPWPGIG
                     PYESYDADHTVEGGEHLSLAGLEIDVLFTPGHSPGHVTYSIPEAELLLSGDVLFQGSV
                     GRVDLPGGDWDTLLESIRMLITTFPEQTRV"
BASE COUNT          422 a          835 c          873 g          393 t
ORIGIN      
        1 ccgggacccg gacggcgacc ctgcccgggg cgacggtgga gatgctttcc cgggtgatga
       61 gcgacgacat ggaaaagctc cgccaccgcg gccaccgcgt ggccgaactg ctcgaccgcg
      121 gcaccgaggc ccggctgacc tgcgatcacg gcagcaacct gaagctgggt cttcaggggc
      181 gggtcgcgat ccccgacgcc ggtgagctga ccgaaaccgg cgccttcggg aacctgcctt
      241 gcggcgaggg attcatggca ccggccactg ccgaaggcgt gctcgtggtc gacggttcaa
      301 tcgccggggt cgggctcgtg gacgagccgg tccggttgac gatcaaggac ggccatctgg
      361 tcgacgcgac cggcgaggcg ggtgcgaagc tgatggaact actgaccgtc catggtccgg
      421 acggcaccaa cgtcgccgaa ctcgggatcg ggaccaacga gaaggcgatc ctgaccggca
      481 acatcctcga ggacgagaag atcctcggca ccgcccatat cgccttcggg gcttccgccg
      541 cgatcggagg aaacgtccag gttccggtcc accttgacgt ggtttcgatg cgaccggaag
      601 tgacgatcga cgggacgccg gtggtgctcg aaggcaggct gctggtgtga gcctgctggc
      661 cgtccccaac gtctccgagg gtcgcgactt cgcccggctc gaccggctgg aggcggcgct
      721 cggccaggag gtcacgctgc tggaccgcca caccgacctt gaccatcacc gggcggtctt
      781 caccctggcc ggaccggccg aaacgctggc cgatgcgctg accggactgg ccggcacggc
      841 gacggtggaa atcgacatga ccgcctggga tggaatgcac ccggcgatcg gcgcgctgga
      901 tgtctgcccg gtggtctggc tgagtgaaag acgccgccag atggccagcg aggtggcgct
      961 cgaagtgggg gagaagatcg gggcgatggg cgttccggtc tttctttacg gcgacctcgc
     1021 gtccgacgcc gaccggcgtg agcgggccta ttttcgggcc ggcgggctcg attccctctg
     1081 gcagagaatg acttccgggg cgctcaggcc ggactttggc ccgaaccagc cgcaccccac
     1141 ggccggggcg accctggtca cagcccgggc gccgatcgcc gccttcaacc tcgaactggc
     1201 caccgacgac ctcgcggtcg cacgcgaaat cgcggctgcg gtcagggagg gaccggatga
     1261 cggcatcccg ggggtccgcg ccctcggcct gctgctttcc acggggcggg cccaggtatc
     1321 gaccaacgtt cacgacccga tcgaaacacc cctggccgag atcgtcgatg cggttcagga
     1381 agaggcgacg ctgaggggaa cctacgtggt cgaggcggag ttgatcggcc tgattcccga
     1441 ggcggcgctg gaggagtatc cggaaggggt gccgatccgg gatttcgatc cggccttcca
     1501 cgtcatcgaa cggcgcctga agcctcgcga ccaagccggc cagtagccgt tctccggtaa
     1561 gtttcgcccg tggcacagac caagaagaag cgcagccgga agaagcgcgg cacccaggcc
     1621 ggcaacctcg acacctcacg ccgttcgcgg ccccggtcca gagccgaagc caggaaccag
     1681 gcgcgttccg gcggcggcaa gggcggccgg accatgcaga agggtgacgc tccgccgacc
     1741 tggcggggag cgaccatccg cgggctgatc gctgcgggcg tctttgtcct gctcctgctg
     1801 ctgcttttcg gccggccgct cgccacttcg ctgatcatcg gcgtcgtgat gctgatcttc
     1861 tacatcccgg ccggctacta catggacctg tatctctggc gccggcgcga acgccagcgg
     1921 atccgcgcca agagcaaggg ttaggcacct ggcatggcgg ggctcgaggt catcggaacc
     1981 acggtcgggc cggtcgcgga aaactcgtac ctggttttca aggaggaaac cggcaaggcg
     2041 cttttgttcg accccggcga cgaggccgac cggctgatgc agccgctggc cgatcgcggg
     2101 ctggagctgg aagcgatcct gcttacccac tgtcacttcg atcacatcgg ggcggtggcc
     2161 ccgatcgcga gggcaaccgg ggcgccggtc tactgcccgt tgatcgaggt gccgatcctg
     2221 gccgacgtga tggcctacgt gccgtggccg ggaatcggtc cctacgaaag ctacgacgcc
     2281 gaccacacgg tcgaaggcgg cgagcatctc tcgctggccg gacttgaaat cgacgtcctc
     2341 ttcacacccg gtcacagtcc gggccacgtc acctactcga tcccggaagc ggagttgctg
     2401 ctttccggcg acgtgctctt tcagggctcg gtcggccgcg tcgacctgcc cgggggtgac
     2461 tgggacacgc tgctcgaatc gatccgcatg ctgatcacca ccttcccgga acagaccagg
     2521 gtg
//
LOCUS       DAOQFI010000250         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22318_length_2523_cov_2.849676, whole genome shotgun sequence.
ACCESSION   DAOQFI010000250 DAOQFI010000000
VERSION     DAOQFI010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22318_length_2523_cov_2.849676"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="PLE93_05255"
     CDS             complement(<1..1928)
                     /locus_tag="PLE93_05255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH86489.1"
                     /translation="MRIALVTPYSWTYQGGVNRHVQSLAEEYISRGHYVRVIAPFDPP
                     DRLSKVLHRAPAEDREIPDYLIPVGRTFGVNANGAVSNIPVFPSGVTQTRRAIEEGNF
                     DVVHLHEPTTPANGWDTCLSSDVPVVGTFHAYSTKPVPNYLANAVGARRTLNRLSARI
                     AVSQAAAWTGRRWYGGNYTIVPNGVDVDAAPDGPKPETDHLRALFVGRPDERKGLPVL
                     LRAFSALVDHVPARLSVVGAGPEDVQRILAHPELADHLDLHGRVSNEELWQQLHDADV
                     LVAPSLSGESFGMILTEAFAVGTPVIASGIAGYSDVVTHNHDGILVPPADPQALAEEL
                     QWAHHEPERLAEMGEAARESAQRYAWSNVADQVMDVYERAIEVPEPEDTVDRVRRRIG
                     IAPSDGLPWAPAVKLPSLDPVPASGPSGGGKSWLRKAGLAAMALFGLGLTAIAAQKIG
                     VDQVADKLVDSDISWVLIALALMASSLFFRAWSWFFIARSAMPHSGLKRRDFASATMI
                     GVLMSATLPARLGEPARAISLARHTGRAKDTLPVVIGTIVSQTMLNILAILLLGAFVL
                     TSMDDVFHGATKQILLYSMIPAALLLAVVTALTKVATGAYAARRDGVGTKGQVRAGAT
                     LIVRGEFSLVIIGLVGET"
     gene            complement(1971..>2523)
                     /locus_tag="PLE93_05260"
     CDS             complement(1971..>2523)
                     /locus_tag="PLE93_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86490.1"
                     /translation="VAGVVATVVFAAGRFGADVGAAIVFPVAAGVGAAFAVGRPKLAW
                     AGLAAAFAALLLVALADIVSGSETHFVRSVLGGSGGSFFEVVGHRLDATLESFTKVSR
                     IPVTLAALAVIAIGIWKREIVLAWFEDSPMMKAGFAAAAAGSLIGALTNDSGALFIQV
                     GTLYLALVAGFAWTTSNYHPESG"
BASE COUNT          454 a          811 c          898 g          360 t
ORIGIN      
        1 atggtctcgc ccaccaggcc gatgatcacc agcgagaact ccccccggac gatcagggtc
       61 gcgcccgcgc gcacctggcc cttcgtcccg acgccgtccc ggcgggcggc gtatgcgccg
      121 gtcgcgacct tggtcagggc ggtcaccacc gccaggagca gggcggccgg aatcatgctg
      181 tagagcagga tctgtttggt cgcgccgtgg aagacgtcgt ccatcgaggt cagcacgaag
      241 gcgccgagca agaggatcgc gaggatgttc agcatcgtct gcgagacgat cgtcccgatc
      301 accacgggca gggtgtcctt ggcccggccg gtgtggcggg ccaggctgat cgccctcgcg
      361 ggttcgccca gacgggcggg caaggtcgcg gacatcagca ccccgatcat cgtggcggaa
      421 gcgaagtcac ggcgcttgag gccggaatgc ggcatcgccg aacgcgcgat gaagaaccac
      481 gaccaggcgc ggaagaagag cgaggatgcc atcagggcaa gcgcgatgag aacccaggag
      541 atgtcggaat cgaccagctt gtcggcgacc tgatcgacac cgatcttctg tgcggcgatc
      601 gcggtgaggc caagtccgaa cagtgccatc gccgcaagac cggccttgcg cagccaggac
      661 ttgccgccgc cggaaggccc ggatgcgggg acgggatcga gcgagggcag tttgacggcc
      721 ggagcccagg gcagcccgtc ggaaggtgcg atgccgatcc ggcgccggac ccggtcgacc
      781 gtgtcctctg gttcgggaac ctcgatcgcc cgttcgtaca catccatcac ctggtcggcg
      841 acgttggacc aggcgtagcg ctgggcgctt tcgcgggcgg cctcgcccat ctcggccaga
      901 cgctccggct cgtggtgggc ccactggagt tcctcggcca gggcctgcgg atcggcgggc
      961 gggacgagga tgccgtcatg gttgtgagtg accacgtcgc tgtagccggc gatgccggaa
     1021 gcgatcaccg gcgtgccgac ggcaaaggcc tcggtcagga tcatgccgaa ggactcgccg
     1081 gaaagcgagg gagcgaccag cacatcggcg tcgtgcagct gctgccagag ctcttcgttg
     1141 gaaacccggc cgtgcagatc gaggtggtcg gccagttcgg ggtgggcgag gatgcgctgg
     1201 acgtcctcgg gaccggctcc aaccaccgaa agccgggccg gaacatggtc gaccagggcc
     1261 gaaaaggcgc gcagcaggac cggcaggccc ttgcgttcat ccggccggcc aacgaagagg
     1321 gcccgcaggt ggtcggtctc cggtttgggc ccgtccggag ccgcgtcgac gtccacaccg
     1381 ttggggacga tcgtgtagtt gccgccgtac cagcgcctac cggtccaggc ggcagcctgc
     1441 gaaacggcga ttcgggccga gagccggttc agggtgcggc gggcgccgac cgcgttggcc
     1501 agatagttcg ggaccggctt ggtcgagtag gcgtggaagg tgccgaccac cggaacgtcg
     1561 ctggacaagc atgtgtccca gccgttggcc ggcgtggtcg gctcgtgcag atggaccacg
     1621 tcaaagttgc cttcttcgat cgcccggcgg gtctgcgtca cgcccgaggg gaagaccgga
     1681 atgttcgaaa cggccccgtt cgcgttgacg ccgaaggtgc ggccgaccgg gatcaggtaa
     1741 tcgggaatct cgcggtcttc ggccggggca cgatgaagaa ccttcgagag gcggtcgggt
     1801 ggatcgaagg gcgcgatgac gcggacgtag tggcctcggg agatgtactc ctccgccagc
     1861 gactggacat gccggttaac gccgccctga taggtccacg aatacggtgt gacgagggcg
     1921 attcgcacga tagaacagga tagtgaaggt ggcggctttc cgagggacgg ttaacccgat
     1981 tctgggtggt aattcgacgt cgtccacgca aatcccgcta ccagcgccag ataaagcgtt
     2041 ccgacctgga tgaagagggc gccggaatcg ttggtcagcg caccgatcag ggagcccgcg
     2101 gcggcggcgg caaagccggc tttcatcatc ggcgaatcct caaaccaggc gagcactatc
     2161 tcccgcttcc agataccgat cgcgatcacc gccagcgcgg ccagggtcac cgggatccgc
     2221 gagaccttgg tgaagctttc cagggtggcg tcgagccggt ggccgaccac ctcgaaaaac
     2281 gaccctccgg acccgcccag caccgacctc acgaagtggg tctcgctgcc gctcacgatg
     2341 tccgccagtg cgaccagcag gagcgcggcg aaagccgcgg cgaggcccgc ccatgccagt
     2401 ttcgggcggc cgacggcgaa ggcggcgcca acccccgccg ccaccgggaa gacgatcgcc
     2461 gcaccgacgt cggcgccgaa ccggccggcc gcgaagacga ccgtggccac caccccggcc
     2521 acg
//
LOCUS       DAOQFI010000251         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22502_length_2511_cov_3.269951, whole genome shotgun sequence.
ACCESSION   DAOQFI010000251 DAOQFI010000000
VERSION     DAOQFI010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22502_length_2511_cov_3.269951"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLE93_05265"
     CDS             <1..807
                     /locus_tag="PLE93_05265"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HNH86491.1"
                     /translation="RHALLDDEAAAQPLLHRGLMRKKWRYVGFYGDEVMLCAAIARIG
                     PFSHSFWSVWDRIEGKVWEHTRLRPGGQEVVLDGPVVEIRTPNVRAAIRFGELEPIEV
                     IAPSGKGWAWTRKRAGVPVSGRIITDGKIREVRGFGVDDESAGYHQRNVKWKWSAGIG
                     EATDGRALGWNLVEGINDSPQQSERAIWLEGVPGEPGPVSFDGLDAIRFGEPDEGLVF
                     DFGGAERKRHDNFGLIRSDYTHRFGTFTGRLDGIELASAAGVTEEHAVVW"
     gene            complement(815..1504)
                     /locus_tag="PLE93_05270"
     CDS             complement(815..1504)
                     /locus_tag="PLE93_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86492.1"
                     /translation="MFESARQRSGGAAGSATLEQVGLILLLAAAFTGLIAATVTGVVE
                     PPGHGLGIRIANRIACGPREPGVCRQHPAVSAYGWPLARALRWLAPDPVARIGPEGTP
                     LAPVDFRYCQRPGCAVPAGDGRLTTANRRLTLFTEVKRLPSGVTGGKSAKLEFTYWFY
                     RPSLGWEKVVRQAGPAEIAAASGTRLLLEDSPRLVPLEILPGRNHYDLPAGDEPPWRW
                     KVTPSYEGWSD"
     gene            1646..1915
                     /locus_tag="PLE93_05275"
     CDS             1646..1915
                     /locus_tag="PLE93_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86493.1"
                     /translation="MEGSPFNMFGDPDELRARMQEMADQMQSSQEVAWADNAIKLAVD
                     MTVASIGRLELTGSSDEQAMQVRDAIRVVFPEAVTLVREARQGLR"
     gene            1960..>2511
                     /locus_tag="PLE93_05280"
     CDS             1960..>2511
                     /locus_tag="PLE93_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH86494.1"
                     /translation="MIHGTGTDSSVFDEAVGSLAGSWRTVTVDRRGWGRSVPPDEYRR
                     TSIAEQAIEVAAVLRELKVEDATLVGLGFGAVVALEIALADPELAARSFLIEPPVFGA
                     LKEATEGMSADVDAIRAAAGEGGEEAAYELFLTGRLHTLGVGAERFADHADRGPAAAR
                     SFLIEIPAVPAWSHDLTAALATAK"
BASE COUNT          425 a          818 c          870 g          398 t
ORIGIN      
        1 cggcacgcgc tcctcgatga cgaagcggca gcgcagcccc tccttcaccg cggcctgatg
       61 cgcaagaagt ggcgctatgt gggcttctac ggcgacgagg tcatgctttg tgccgcgatc
      121 gcccggatcg gtcctttcag ccacagcttc tggtcggtct gggaccggat cgagggcaag
      181 gtctgggaac acaccaggct caggcccgga gggcaggaag tggtactcga cggtcccgtg
      241 gtcgagatcc ggacccccaa cgtgagggcc gcgatccgct tcggtgagct cgaaccgatc
      301 gaagtgatcg ccccgagcgg gaagggatgg gcatggaccc gcaagcgggc cggagtgccg
      361 gtcagcgggc gaatcatcac cgacgggaag atccgtgaag tgcgcgggtt cggagtcgac
      421 gacgagtcag ctgggtacca ccagcgcaac gtcaaatgga agtggtcggc gggaatcggc
      481 gaggccactg acggccgggc gctcggctgg aacctggtcg aaggaatcaa cgactctccc
      541 cagcaaagtg aacgggcgat ctggctcgag ggcgtgcccg gtgagccggg accggtcagc
      601 ttcgacggtc tcgatgcgat ccggttcgga gaaccggatg aaggcctcgt tttcgacttc
      661 ggtggagccg aacgaaaacg ccacgacaac ttcgggctga tccgctccga ctacacccac
      721 cgcttcggca cattcaccgg aaggctcgac ggcatcgaac tcgcctcggc cgccggggtg
      781 acggaagaac acgcggtcgt ctggtgagcc ggtctcagtc cgaccagcct tcgtagctcg
      841 gggtcacctt ccagcgccag gggggttcgt caccggccgg gaggtcgtag tggtttcggc
      901 cgggcaggat ttccagaggc accagacgcg ggctgtcctc gagcagcagc ctggttccgg
      961 aggccgccgc gatctcggcc ggaccggcct gccggaccac cttctcccag cccagcgagg
     1021 gccggtagaa ccagtaggtg aactccagct tcgccgattt gccgccggtc acgccggacg
     1081 gcaaccgctt cacctcggtg aagagggtga gcctgcggtt ggcggtggtc agcctgccgt
     1141 cgccggccgg caccgcgcag cccggtctct ggcagtagcg gaagtcgacc ggggccagcg
     1201 gcgtcccttc ggggccgatc cttgcgacgg ggtccggcgc cagccagcgc aatgcccggg
     1261 cgagcggcca gccgtaggcc gagaccgccg gatgctggcg acagaccccc ggctcgcgtg
     1321 ggccgcaggc aatccggttg gcgatcctga ttcccaatcc gtgccctggc ggttcgacga
     1381 ctccggtcac ggttgcggcg atcaggccag tgaaggcggc agccagaagc aggatcagcc
     1441 cgacctgctc gagggtcgcc gatccagccg ccccgccgct tctttgcctt gccgattcga
     1501 acatgacccg aagctacgga cgaattgctc acatgtggct ctacaggtgc aacgattcag
     1561 ttacaggcga gcggaaagga agcgcatccc gaccgtgagg gccgtatctg cgcggccgga
     1621 cactgcgggt cgctagattg gtctcatgga aggttcaccg ttcaacatgt ttggcgaccc
     1681 cgatgaactg cgtgcccgga tgcaggagat ggccgaccag atgcagtcct cccaggaagt
     1741 cgcctgggcc gacaacgcga tcaagctcgc cgtcgacatg accgtcgcct cgatcggcag
     1801 actggagctc acgggaagct ccgacgagca ggcgatgcag gttcgtgacg cgatccgcgt
     1861 ggtctttccc gaggcagtga ccctggtccg ggaagctcgc cagggactcc gctgagcgat
     1921 ctcaacttca tcgaccgggg cgaagggccg ccggtcctgc tgattcacgg aaccggcacc
     1981 gattcctcgg tgttcgacga ggcggtcggc tcgctggccg gttcgtggcg aacggtcacg
     2041 gtcgaccggc gcgggtgggg ccggtcagtt ccgccggatg aataccggcg gacctcgatc
     2101 gctgaacagg cgatcgaagt cgctgcggtc ctgcgcgaac tgaaggtcga ggatgccacc
     2161 ctggtcgggc tcggtttcgg cgcggtcgtg gccctggaga ttgccctcgc cgaccccgaa
     2221 ctggcagctc gctccttcct gatcgagccg cccgtcttcg gcgccctgaa ggaagcgacc
     2281 gaggggatgt cagcagacgt cgacgcgatc cgggcggccg ccggagaggg cggggaggaa
     2341 gccgcctacg aactctttct gaccggccgg cttcacaccc tcggggtcgg ggccgaacgc
     2401 ttcgccgacc acgccgaccg gggtccggcc gcggcccgtt cattcttgat cgagattccg
     2461 gcggttcccg cctggtccca cgatctcacc gcggcgctcg ccacggcgaa g
//
LOCUS       DAOQFI010000252         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22522_length_2510_cov_3.088798, whole genome shotgun sequence.
ACCESSION   DAOQFI010000252 DAOQFI010000000
VERSION     DAOQFI010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22522_length_2510_cov_3.088798"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLE93_05285"
     CDS             <1..809
                     /locus_tag="PLE93_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86495.1"
                     /translation="AAGSARLFVLARVAEQRGTAPEDFDAEVAEAREEFSLSHGAGFV
                     GSVFLIMFSEQVFLNAGPLVVRAVEGVAAAGYIFNVLMIARAPLQLFQAVSTSILPHL
                     TALHHSSDPNSEKEFHHSVRMVLLGIAAFTALTAVVVMIAGPKLMQIAFSDKFTYDRL
                     GLMLVTAGMGLYLAAVTVNQACLAQGQARRSSIRWISCAVFFIIWTILPFISDGNLRV
                     EIGFALTALLLLSLLTFIYFRPKGVEADVPLEGSSEAIEARLAGLEDTST"
     gene            complement(892..1605)
                     /gene="rpe"
                     /locus_tag="PLE93_05290"
     CDS             complement(892..1605)
                     /gene="rpe"
                     /locus_tag="PLE93_05290"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNH86496.1"
                     /translation="MTNQPRNSETPVPEAFQGIRIAPSILSADFGRLGSQIAEIMDAG
                     ARVIHFDVMDGQFVPPITIGPLVLSGIADQVHDAGGVVDVHLMIEDPDRQFEDFARAG
                     ADSIIFHEEATPHAHRCCHAIRELGCLAGVAINPGTPVETVSELKGMVDEILVMTVNP
                     GWGGQPFIETSPDKVRRMRSLVGDDVLIEVDGGIGTGTAGTMAAAGATLMVAGSAIFG
                     SDDPAQAYRDIHAAAESGA"
     gene            complement(1625..1864)
                     /locus_tag="PLE93_05295"
     CDS             complement(1625..1864)
                     /locus_tag="PLE93_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86497.1"
                     /translation="MATESEKTTVDRRPACPGCGEPWLRPTQLPGRFRCVYCLSRYEL
                     VSQCPNCGEHQTIARMSTTEDMSCQSCGASMLQPI"
     gene            complement(2013..>2510)
                     /locus_tag="PLE93_05300"
     CDS             complement(2013..>2510)
                     /locus_tag="PLE93_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86498.1"
                     /translation="NMVSVEPDEERAHEVAAQLERLGFHHGLVVEVDARHGEIMSGFD
                     RVLVDAPCSDLGALASRPDARWRKSPALIERVAELQGEILDRGAGFLREGGSLVYSTC
                     TISKRENSDQALALAKEHGLTIDDLGGRAPQLADPHDSRFLQLMPDRDHTTGFFIARF
                     ISGGD"
BASE COUNT          410 a          826 c          825 g          449 t
ORIGIN      
        1 aggctgccgg ctcagcccgg ctgttcgtcc ttgcccgggt tgccgaacag cgcggcacag
       61 cgccggagga cttcgacgcc gaggtcgccg aggcccggga ggagttctcg ctttcacacg
      121 gcgccggctt cgtcggctcg gtcttcctga tcatgttcag cgagcaggtc tttctcaacg
      181 ccggcccgct ggtggtcagg gcggtcgaag gagtggccgc ggccggctac atcttcaacg
      241 tcctgatgat cgcccgggcc ccgctgcagc tctttcaggc cgtttcgact tcgatcctcc
      301 cccacctgac cgcgctgcac cacagctcgg atccgaactc ggagaaggag ttccaccact
      361 cggtccggat ggtgctgctc ggaatcgccg ccttcactgc gctgaccgcg gtcgtggtga
      421 tgatcgccgg cccgaagctg atgcagatcg ccttctcgga caagttcacc tatgaccggc
      481 tgggcctgat gctggtcacc gccggcatgg gcctgtacct ggccgcggtc acggtcaacc
      541 aggcctgcct cgcccagggc caggcccggc gttcctcgat ccgctggatc tcctgtgcgg
      601 tcttcttcat catctggacg atcctcccgt tcatcagcga tggcaacctg cgggtcgaga
      661 tcggcttcgc cctgaccgcc ctgctcctgc tcagcctgct gaccttcatc tacttccggc
      721 cgaagggcgt cgaagcggac gtgcctctcg aaggctcatc cgaggcgatc gaggccagac
      781 tcgccggcct cgaagacact tcgacctagg tcgcctcaat cagtcttgaa gttattcgct
      841 ccatgtgcga ataacttcaa cgctgatctg agcggtcgag ccccgaagga ctcaggcacc
      901 ggattccgcg gcagcgtgaa tgtcgcggta agcctgagcc gggtcgtcgg aaccgaagat
      961 cgccgagccg gcgaccatca gggtcgcgcc ggccgcggcc atcgttccgg cggtcccggt
     1021 gccgatgccg ccgtcaacct cgatcaggac gtcatcaccg accagcgagc gcattcgcct
     1081 gaccttgtcc ggcgaggttt cgatgaaggg ctggccgccc cagccggggt tcacggtcat
     1141 gaccaggatc tcgtcgacca ttcccttcag ctcggaaacc gtttcgaccg gagtgccggg
     1201 gttgatcgcg acgccggcca gacagcccaa ctcgcgaatc gcgtggcagc agcggtgggc
     1261 atgcggggtc gcttcctcat ggaagatgat cgaatccgcc ccggcccggg cgaagtcctc
     1321 gaactgacgg tcgggatcct cgatcatcag gtggacgtca accaccccgc cggcgtcatg
     1381 gacctgatcg gcgatgccgg aaagcaccag cgggccgatc gtgatcggcg ggacgaactg
     1441 gccgtccatc acgtcgaagt gaatgaccct ggcgccggcg tccatgatct cggcgatctg
     1501 cgagcccagc cggccgaagt cggcggagag gatcgaagga gcgatgcgga tgccctggaa
     1561 agcttcgggc acaggggttt cggagttgcg gggctggttg gtcatcctgg gatccggtcg
     1621 gggttcagat tggctggagc atcgaggcgc cgcagctctg gcagctcatg tcctcggtgg
     1681 tgctcattct ggcgatcgtc tgatgttcac cgcagttcgg gcactgggaa accagttcgt
     1741 agcgggaaag gcagtacaca cagcggaacc ggccgggaag ctgagtcggt cgcaaccagg
     1801 gttccccgca gcccggacag gccggacggc ggtcgactgt tgtcttctct gactcggtcg
     1861 ccatggggcg attatcccgg accggtcgga aactcggcgc gcctccccgt tccgggggcg
     1921 tttggccacc gaaaaggtgg gtgatcgccc ccggaacggg ctggcacgaa cgccaccgga
     1981 cttccggggg cgttcggcca cggatccagt ggtcaatcgc ccccggaaat gaagcgggcg
     2041 ataaagaagc cggtcgtgtg gtcgcgatcc ggcatcagct ggaggaagcg ggagtcgtgc
     2101 gggtccgcca gctgcggggc gcgtccaccg agatcatcga tcgtgaggcc atgctctttc
     2161 gccagggcaa gggcctgatc gctgttttcg cgtttcgaga tggtgcaggt cgagtagacg
     2221 agacttccgc cctcgcggag aaagccggcc cctcgatcga gaatctcgcc ctggagctcc
     2281 gccacccttt cgatcagggc cggggacttc cgccagcggg catccggccg ggaagcgagc
     2341 gcgcccaggt cggagcacgg tgcgtcgacc agaacccggt cgaagccgct catgatctcg
     2401 ccgtgcctgg cgtccacttc gaccacgagc ccgtgatgga agccgaggcg ctcgagctgg
     2461 gccgcgacct catgggcccg ctcttcatcc ggctcgaccg acaccatgtt
//
LOCUS       DAOQFI010000253         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22618_length_2505_cov_3.029796, whole genome shotgun sequence.
ACCESSION   DAOQFI010000253 DAOQFI010000000
VERSION     DAOQFI010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22618_length_2505_cov_3.029796"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /gene="pth"
                     /locus_tag="PLE93_05305"
     CDS             <1..461
                     /gene="pth"
                     /locus_tag="PLE93_05305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNH86499.1"
                     /translation="RAKDKFGARITEGRIRPGGPRVVLMTPLTYMNESGKSAGPARGS
                     KKIPLDRVIAVYDEIDLPFGEIRTRLGGGAAGHNGVKSLKQGFGSAGFWRVRIGVDRP
                     DSTDPERVAAYVLGKFSESREQVEELIDDGARETERLVERIAAGETVDGD"
     gene            483..1193
                     /locus_tag="PLE93_05310"
     CDS             483..1193
                     /locus_tag="PLE93_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH86500.1"
                     /translation="MSLTTYGVDDRGVALIQLDRPKAMNAINTEMLVELIEHVAVARS
                     DEGVKVLVFSSTDYMAFSAGADIKEDIDDDAKVNKMQLFADLYDAVVQFPKPTIAACH
                     GYVVGGGAEIAAACDIRIGGSNLQMRFPGAALGVPVGPARLVTLCGLSHAKYLLLSSN
                     TVKVDEALRMGLINQAAPGAATENTALELAGRIAAHDPAGVARLKRMLHEWDDIEGRS
                     EAEGRGQVEWQRSGPGLH"
     gene            complement(1335..>2505)
                     /locus_tag="PLE93_05315"
     CDS             complement(1335..>2505)
                     /locus_tag="PLE93_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86501.1"
                     /translation="QGRLLREALEAIGPGDSPYRAQLLSSLAQILYFSDGRSAEAREM
                     ALEGTGMADRLDDPYTFFVSNIAATFLRWEPDGTESRLPMADRLVEVAESSEDPEDLA
                     EALSWRSVVQVCLGHRAEADADLARFDVLAKTLPQIRVNQAALHTARKVREGRWAEAE
                     QLIAKTIAQEIPNTARIALTDPLLFMIRAEQNRLGEHFDQVKAMAAQSTSWEHWPTWR
                     MGYYLAMTQCGRGDEVLERVDRFNQADVEAIETRNITFLSFCALGSMVACEIEFRRGA
                     ETLVNLMEPYVGEWVITGPAGSALGPVEMHLGEICLLLGRDREAATWLERSVDNCEGM
                     GARPYLAHSKMHLALALRRLGNPEPERAEELMSSGQQIAEELDMQMLLNRINRWS"
BASE COUNT          430 a          805 c          844 g          426 t
ORIGIN      
        1 cgcgggccaa ggacaagttc ggcgccagga tcaccgaggg ccggatccgg cccggcggcc
       61 cgcgagtggt gctgatgacc ccgctcacct acatgaacga gtccgggaag tcggccggcc
      121 cggctcgcgg gtcaaagaag atcccgctcg accgggttat cgccgtctac gacgagatcg
      181 acctgccctt cggtgagatc cgcacccgcc tcggcggcgg tgccgccggc cacaacgggg
      241 tcaagagcct gaaacagggt ttcggatcgg ccggtttctg gcgggtccgg atcggggtcg
      301 atcgacccga ttcgaccgat cccgaacgcg tcgcagccta cgtgctcggg aagttctccg
      361 aaagccggga gcaggtcgag gaactgatcg atgatggggc cagggaaacc gaaagacttg
      421 tcgagcgaat cgccgccggg gaaaccgttg acggcgactg aaccgaggaa agtgaagctg
      481 ccatgagcct gaccacctat ggagtagacg accgcggagt tgccctgatt cagctcgacc
      541 ggcccaaggc gatgaacgcg atcaacacgg aaatgctggt cgaactgatc gagcacgtcg
      601 cggtggcccg cagtgacgaa ggcgtaaagg tgctggtctt ctcctcgacc gattacatgg
      661 ccttctcggc cggcgccgac atcaaggaag acatcgatga cgatgccaag gtcaacaaga
      721 tgcagctctt cgccgacctc tacgacgcgg tcgtccagtt tcccaaaccg accatcgccg
      781 cctgccacgg ttacgtggtc gggggcggag ccgagatcgc ggccgcctgc gacatccgga
      841 tcggcggctc gaacctgcag atgcgattcc ccggggcggc gctgggagtg ccggtcggcc
      901 cggcccggct ggtcaccctt tgcgggctca gccacgccaa gtacctgctg ctctcctcga
      961 acacggtcaa ggttgacgaa gccctgagga tgggcctgat caaccaggcc gcacccggtg
     1021 ccgcaaccga gaacaccgcg ctagagctgg ccggccggat cgccgcccat gaccccgcag
     1081 gggtcgcccg actgaagcgg atgctccacg agtgggatga catcgagggg cggtcggaag
     1141 ccgaaggtcg gggccaggtc gagtggcagc gttcgggtcc aggcttgcat tgagggcccg
     1201 gtgtcggggg tgcctgaaaa gtaacgagcg cttcgcgctc tgattaagga gtcacttttc
     1261 aggcaccccc gacaccgggc cccgcattag ggagccggcg agcttcgctc cggctctgcc
     1321 gcggttcggg tctgtcagga ccagcggttt atgcggttca ggagcatttg catgtctagt
     1381 tcttcggcta tttgttggcc ggagctcatt agttcttcgg ctctttccgg ttcggggttg
     1441 ccgaggcgcc ggagggcaag agcgaggtgc atcttcgagt gggcgaggta gggccgggcg
     1501 cccatccctt cgcagttgtc gaccgagcgt tccagccagg tggcggcctc acggtcccgg
     1561 cccagcagca ggcagatctc gcccaggtgc atttccaccg ggccgagcgc cgaccctgcc
     1621 ggtccggtga tcacccattc accgacgtaa ggctccatca gattgaccag ggtttccgcc
     1681 cctcgcctga actcgatctc acaggcgacc atcgagccga gcgcgcagaa tgagaggaag
     1741 gtgatgttgc gggtttcgat cgcttccacg tccgcctggt tgaaccggtc gaccctttcg
     1801 aggacctcgt ccccgcggcc gcactgggtc atcgccaggt agtagcccat ccgccaggtc
     1861 ggccagtgct cccagctcgt tgactgggca gccatcgcct tgacctggtc gaaatgctcg
     1921 ccgagccggt tctgctcggc ccggatcatg aagagcagcg ggtcggtcag ggcgatccgg
     1981 gcggtgttcg ggatctcctg cgcgatggtt ttcgcgatca gctgctcggc ctcggcccag
     2041 cgtccttcgc ggaccttccg ggcggtgtgg agggcggcct gattgacccg gatctggggc
     2101 agggtcttgg ccaggacatc gaagcgggcc aggtcggcat cggcctcggc ccggtgaccg
     2161 agacagacct gaacgaccga ccgccagctc agcgcttctg ccaggtcttc cggatcctcg
     2221 gaagattcgg ccacctcgac cagccggtcc gccatcggca gcctggattc ggtcccgtcg
     2281 ggttcccagc ggagaaaggt ggcggcaatg ttggagacga agaacgtgta cgggtcatcc
     2341 agccggtcgg ccatcccggt gccttccagg gccatctccc gggcttcggc ggaacgaccg
     2401 tcgctgaagt agagaatctg ggcgagcgat gacagcagct gggcccggta cgggctgtcc
     2461 ccggggccga tcgcctccag cgcctcccgc agcagccggc cctgt
//
LOCUS       DAOQFI010000254         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22662_length_2503_cov_2.852124, whole genome shotgun sequence.
ACCESSION   DAOQFI010000254 DAOQFI010000000
VERSION     DAOQFI010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22662_length_2503_cov_2.852124"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLE93_05320"
     CDS             <1..861
                     /locus_tag="PLE93_05320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNH86502.1"
                     /translation="RSMTKKPFGVNITVFPTTMSPNYKAYAQVALDAGVRIFETAGTQ
                     AVAEVWEVLRTKDTCVIHKCTAVRHAVSAEKKGVSAISIDGFECAGHPGEDDIPGLIL
                     IPCAADKVKIPLLADSGCSRYGASFHSSTQLCAGYLRGGRDACQGDSGGPLSVTADHG
                     ERRLVGLVSTGRGCAEAGFPGIYSKVAGAVNSDRIQQEVEFIETEDDFGAAYTGINVI
                     GSGARPLGCSAAVKSGKTAAQKLKSAQRRLKAARRSGRANRIEAARRGVERARHKLGR
                     SRVKSRKACT"
     gene            858..2378
                     /locus_tag="PLE93_05325"
     CDS             858..2378
                     /locus_tag="PLE93_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86503.1"
                     /translation="MNFPIRNLMTKSLAGALALAGLAAIVLAPTASAAPIANAGEAPS
                     LLDFSGKAFKATPVAGASKAPQNPFMAKNQKSNIHNDTWMTGTYTWPGPLGRNLVTNS
                     GSAANGVCSTIAFDSHGDIVTVCVSTIAAPQARIVDPDTLEVIDTYDLPNADPVGNTP
                     AYQNFSAGGYFYLDNQDRLVIPTRNNHIYVLGQTPDGSQFQLVKDYDVTSVVDPATER
                     MNSALPDFQGDIWFVVKQTGEVGTVDRKTGQVRYLRLGVGIQNSFTVDRNAVYVVTSK
                     RIYRMWKNGNGKPYVVWSAKYRNTGVQKPGQADAGTGTTPDVMSGGYVSMVDNANPEN
                     IVVYRTAAKLHGKKRKVCEVPVFGKDASATENSLITTGRSMIVENNYGYTNIFDPGAG
                     AVVTRAGFARVDIRKDGKGCKKIWTNKTERAPSVVPKMSAKTGLIYSYTRPPDPSGAQ
                     GYYWSAIDYRTGKTVWNKYAGSGFLYNNNYSGLGLGPDGTAYLGVIGGIISLKDGS"
BASE COUNT          504 a          828 c          781 g          390 t
ORIGIN      
        1 cgctcgatga cgaagaagcc gtttggcgtg aacatcaccg tgttcccgac gacgatgtcg
       61 ccgaattaca aggcctatgc ccaggtcgcg ctcgacgccg gcgtacgcat cttcgagacc
      121 gccggcacgc aggctgtggc cgaggtctgg gaggtgctgc gcaccaagga cacctgcgtc
      181 atccacaagt gcacggccgt ccgccacgcg gtctcggcgg agaagaaggg cgtttcggcg
      241 atctcgatcg acggcttcga atgcgccggt cacccgggcg aagacgacat cccgggcctg
      301 atcctgattc cgtgcgcagc cgacaaggtg aagatcccgc tgctggccga ctccggatgt
      361 tcccggtacg gggcgagctt ccactcttcc acgcagctct gcgccggcta cctgcgaggc
      421 ggcagggacg cctgccaggg tgacagcgga gggccgctga gcgtcaccgc ggatcacggc
      481 gagcgcaggc tggtcggcct ggtcagcacc ggacggggtt gtgccgaggc cggcttcccg
      541 ggcatctact cgaaggtcgc cggggccgtg aacagcgacc gcatccagca ggaagtcgag
      601 ttcatcgaaa ccgaggatga tttcggcgcc gcctacaccg ggatcaacgt gatcggctcc
      661 ggtgccaggc cgctcgggtg ttcggccgcg gtcaaatcag gaaaaaccgc cgcgcagaag
      721 ctaaagtcag ctcagcgaag gctcaaggcc gctcgcaggt cggggagggc aaaccgaatc
      781 gaagctgcgc ggcggggtgt tgaaagagcc aggcacaagc tcggcaggag ccgggtgaaa
      841 tcgagaaagg catgcacttg aacttcccga tccgcaatct gatgacgaag tcgctggccg
      901 gcgccctggc cctggccgga ctggccgcga tcgtccttgc ccctaccgca tcggccgcgc
      961 cgatcgccaa cgcgggtgaa gccccctcgc tgctcgactt ttccggcaag gccttcaagg
     1021 cgaccccggt cgccggcgcg tcgaaggcgc cgcagaaccc gttcatggcg aagaaccaga
     1081 agagcaacat ccacaacgac acctggatga ccggcaccta cacctggccc ggcccgctcg
     1141 gccggaacct ggtcaccaac tcgggttccg ccgcgaacgg cgtctgctcg accatcgcct
     1201 tcgacagcca cggcgacatc gtcacggtct gcgtttccac gattgccgca ccgcaggccc
     1261 ggatcgtcga cccggacacg cttgaggtga tcgataccta cgaccttccg aatgcggacc
     1321 cggtcggaaa caccccggcg taccagaact tttcggccgg cggctacttc tatctcgaca
     1381 accaggaccg gctggtcatt ccgacccgca acaatcacat ctacgtgctc ggccagaccc
     1441 ccgacggaag ccagttccag ctcgtaaagg attacgacgt gacctcggtc gtcgacccgg
     1501 caaccgaacg catgaactcg gccctgcccg atttccaggg tgacatctgg ttcgtcgtca
     1561 agcagacggg cgaggtcggc acggtcgatc gcaagaccgg ccaggtcagg tacctcaggc
     1621 tcggcgtggg gatccagaac tcgttcaccg tcgaccggaa cgcggtctat gtagtgacca
     1681 gcaagcggat ctaccggatg tggaagaacg gcaacggcaa gccctatgtc gtctggagtg
     1741 cgaagtaccg gaacaccggc gtccagaagc ccggacaggc cgatgccggc accggtacga
     1801 ctcccgacgt gatgagtggc ggctacgtat ccatggtcga caacgccaat ccggagaaca
     1861 tcgtggttta ccggaccgcg gcgaaactcc acggcaagaa gcggaaggtc tgcgaggtcc
     1921 cggtcttcgg caaggacgcc agcgccaccg agaactcgct gatcaccacc ggccgctcga
     1981 tgatcgtcga gaacaactac ggatacacga acattttcga tcccggagcc ggtgccgtgg
     2041 tgaccagggc cggctttgcc cgggtcgaca ttcgcaagga cggcaagggc tgcaagaaga
     2101 tctggaccaa caagaccgag cgggcgcctt cagtggttcc gaagatgtca gcgaagaccg
     2161 gcctgatcta ctcctacacc cgcccgcctg atccgagcgg cgcccagggt tactactggt
     2221 cggcgatcga ctaccgcacc ggcaagacgg tctggaacaa gtacgcgggc tccggcttcc
     2281 tctacaacaa caactactcg ggtctcggtc tcggccccga cggcaccgcc tacctcggag
     2341 tgatcggcgg catcatctcc ctcaaggacg gcagctagcc cgaatcagtt ccagcgttcc
     2401 ccaggccgtc gagtaacgat gctcgacggc cgggtttgta ccaccgccaa acggagcccg
     2461 cccatccata aatttgttca gtgggcgggc gggtttcgac tgg
//
LOCUS       DAOQFI010000255         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22739_length_2498_cov_3.407695, whole genome shotgun sequence.
ACCESSION   DAOQFI010000255 DAOQFI010000000
VERSION     DAOQFI010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22739_length_2498_cov_3.407695"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PLE93_05330"
     CDS             complement(<1..1077)
                     /locus_tag="PLE93_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86504.1"
                     /translation="MKRATGCVIAVIVFCLLGASSASGYLVYYKEGNTNVDTGSGLVW
                     AVKPSATGQNNWWWYINNNLNVDTAVCYRYQIDGGGWKAPNGSNYSAPTGNNTGTLPA
                     ANASICEGPYTDGTKGPLQFAKSLTGLVNGQIATYCAEEWFKSGGDWDNSADPEQCSS
                     VKIDTVSPTITVDLRQGGNPVSATNSPGSVTDNIGYSDSASQPWNRGSVWGDNRLCTT
                     TSMACNSGNTFNFSATCSNGTKSLSQTFTCPMGTPPGDGTFYQCIAGFDGAVLDTYPS
                     LIADNVAGQPNTFTNDDPTGIGNTPANTIYPQTFNAGTGNYGIGCDSVVIDTAAPNTS
                     ITGGPAEGSSSADTTPTFTFTSTEA"
     gene            complement(1173..>2498)
                     /locus_tag="PLE93_05335"
     CDS             complement(1173..>2498)
                     /locus_tag="PLE93_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86505.1"
                     /translation="LMGPTASGKTALACALAETSLMLNLLRAVQGIGGGMMFSTSLAI
                     IAQTFQGRDRGIAFGIVGATTGAAVAIGPLLGGLLTDAFGWEAIFFVNVPIGIFALIG
                     TFRNVDESKDPHPSGIDWLGAVLFSAALFLMIYALVHGNDKGWGSLEIIGAMVLSVVL
                     LIIFGGVETHKEHPLFDLSLFRKPAFVGACLVAFCMSSSLFALFLFLTFYIQGGVLGF
                     SPLEAGLRFLPITGMMLVAGPIAGRLTTKVQVRYLLRGGLLGVALGIFLLHGIDVNSG
                     WTALILGFVVAGVGSGFVNPPLASTAIGVVEQRRSGMASGINSTFRQAGIAAGIAGLG
                     AIFQSRVGNLLAEQLAGTRAAPHSDQISAAVTAGQTEQAISHIPGSVGAAQIDQIREA
                     AHFAFVGGMNTLFLVTSAIALVGSVGAFFLVRQSDFVAPPGQEGGTGTE"
BASE COUNT          443 a          744 c          881 g          430 t
ORIGIN      
        1 tgcttcggtc gaggtgaagg tgaaggtcgg ggtggtgtcg gcgctgctcg agccttcggc
       61 cgggccgccg gtgatcgagg tgttcggggc ggcggtgtcg atcacgacgc tgtcgcagcc
      121 gatcccgtag ttgccggtgc cggcgttgaa agtctgtgga tagatcgtgt tggccggggt
      181 attgccgatc ccggtcgggt cgtcgttggt gaatgtgttg ggctgaccgg ccacgttgtc
      241 cgcgattagc gacgggtagg tgtcgaggac agccccatcg aaaccggcaa tgcattgata
      301 gaaggttccg tctccgggtg gggtacccat cgggcaggtg aaggtctggc tgagtgactt
      361 ggtgccgttc gaacaggtgg ccgagaagtt gaaggtgttg ccggaattgc aggccatgct
      421 cgtggtcgtg caaaggcggt tgtcacccca gaccgatccc cggttccagg gctggctggc
      481 ggaatccgag tagccgatgt tgtccgtcac cgaacccggc gagttcgtag ccgacacggg
      541 atttccgcct tgtctgaggt cgacggtgat cgtcggggaa accgtgtcga tcttgacgct
      601 cgaacattgc tccgggtcgg ccgagttgtc ccagtccccg ccgctcttga accactcttc
      661 ggcgcagtag gtcgcgatct gtccgttgac gagtccggtc aggctcttgg cgaattgaag
      721 ggggcccttg gttccatcgg tgtaggggcc ctcacagatc gaggcattgg cggcgggcag
      781 ggtgccggtg ttgttgccgg tcggagccga gtagttgctt ccgttgggag ctttccagcc
      841 accaccgtcg atctggtagc ggtaacagac cgccgtgtcc acgttgaggt tgttgttgat
      901 gtaccaccac cagttgttct ggccggtggc acttggctta acggcccaga cgagcccgga
      961 gccggtatcg acgttggtat tgccctcttt gtaatagacg agatagccgg aggctgaact
     1021 cgcgccgagc aggcagaaga cgatgactgc gatgacacaa cccgttgcgc gcttcatgac
     1081 gactccccca atgcgtccgg atcccgatgg tccatccaga ctagcgtcgg tcgaggcgca
     1141 ttgccagcaa tccggagtac cggattgagc ggctattcgg tgccggttcc accctcttgt
     1201 ccgggcgggg cgacgaagtc gctttgccgg accaggaaga aggcaccgac cgagccgacc
     1261 agggcgatcg cgctggtcac caggaagagc gtgttcatcc cgccgacgaa ggcgaagtgg
     1321 gcggcttccc ggatctggtc gatctgcgcc gccccgaccg aaccggggat gtgggagatc
     1381 gcctgctcgg tctggccggc cgtgaccgcg gcggagatct ggtcgctgtg cggagccgcc
     1441 ctggtgccgg ccagctgttc ggccaggaga ttcccgaccc ggctctggaa gatcgccccc
     1501 agcccggcaa tgcccgcagc gatgccggcc tgccggaagg tcgagttgat gccggaagcc
     1561 attcccgacc gtcgctgctc gacgacgccg atcgcggtcg aggccagggg cgggttgacg
     1621 aagccgctgc cgacaccggc cacgacgaaa ccgaggatca gggcggtcca gccggagttc
     1681 acatcgattc cgtgcagcag gaagatgccg agcgcgacgc cgagcaggcc gccgcgcagc
     1741 agatagcgaa cctggacctt ggtggtcagg cggccggcga tcgggccggc caccagcatc
     1801 atgccggtga tcggcaggaa cctgagaccg gcctccaggg gagagaagcc gagcacgccg
     1861 ccctggatgt agaaggtgag gaagaggaag agcgcgaaca gcgatgacga catgcagaag
     1921 gccacgaggc aggcgccgac gaaggccggc ttgcgaaaga ggctcaggtc gaagagcgga
     1981 tgttccttgt gggtttcgac cccgccgaag atgatcagca ggacgaccga gagcaccatc
     2041 gcgccgatga tttccaggct gccccagccc ttgtcgttgc cgtgaacgag cgcgtagatc
     2101 atcaggaaga gcgcggccga gaagagcacg gcgccgagcc agtcgatccc cgacggatgc
     2161 gggtccttgg actcgtcgac gttgcggaag gtgccgatca gggcgaagat cccgatcggc
     2221 acgttgacga agaagatcgc ttcccagccg aaggcgtcgg tcagaaggcc cccgagcagc
     2281 gggccgatcg cgaccgccgc cccggtggtc gccccgacga tcccgaaggc gatgccccgg
     2341 tccctgccct ggaaagtctg ggcgatgatc gccagggaag tggagaacat catcccgccg
     2401 ccgatcccct ggaccgcacg gagcaggttc agcatcagcg aggtctcggc cagggcgcag
     2461 gccagcgcgg tcttgcccga cgcggtcggg cccatcag
//
LOCUS       DAOQFI010000256         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22852_length_2492_cov_4.407058, whole genome shotgun sequence.
ACCESSION   DAOQFI010000256 DAOQFI010000000
VERSION     DAOQFI010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22852_length_2492_cov_4.407058"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLE93_05340"
     CDS             <1..379
                     /locus_tag="PLE93_05340"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HNH86506.1"
                     /translation="SSAATLAAENATRAGQITSLKGDIAGWQKQMEKQQQVSAEQAQV
                     EVEQTLGGPYSIPTYIVMCESGGDYSAVNPSSGAGGAYQIMPSTWEAYGGTGLPQDAS
                     KAEQDRIAALIWADSGSSPWTCG"
     gene            complement(442..900)
                     /locus_tag="PLE93_05345"
     CDS             complement(442..900)
                     /locus_tag="PLE93_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNH86507.1"
                     /translation="MIYLLRHGDAEPDQGSGDAARRLTEKGEAQALAAGAAMAKLGMT
                     VDTCLASPRVRAFDTATLACRSLGIKVEVSEPIGYGDYDSLELAAGRGDTLIVGHEPV
                     LSMEVARLTGANIKMKKGGLALIKGSHLHALLRPTELIEIATGGARSSNI"
     gene            940..1737
                     /locus_tag="PLE93_05350"
     CDS             940..1737
                     /locus_tag="PLE93_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH86508.1"
                     /translation="MTEAAAVPDFSTLLVSAEGPIGRLTLNRPEKLNALSGELLNELI
                     LASKWFTDRTGVKVVLVSGAGRAFCAGADLTSFSAAGSDPSEEERRDGADLGRRMADA
                     VTGMRPMTVAAIQGHCVGGGLVLAAACDLRIAAEDTRFSIPEVDLGIPLAWGGIPRLV
                     REIGPAATKELVLTCREFDAREARELGFLNRTVDPDRLEGQAWELAEKLASQPAYSLE
                     LTKRHVNAVAEESGSTADAITDADGLLGALGDPESLRKMGEYLARRG"
     gene            1843..>2492
                     /locus_tag="PLE93_05355"
     CDS             1843..>2492
                     /locus_tag="PLE93_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNH86509.1"
                     /translation="MSSSASSHGQVVHLHVHSEHSVLDGACNIKEMAARAAELEMPAL
                     ALTDHGVMNGALDMYKACRANDIEPILGIEAYYVDDRIAVKETAGRYERNHLTLLAEN
                     NTGFANLVALSSEGFLEGYHRGKANVDMELLERHSEGVIALTGCLQARFVKRLVEDRP
                     DEAREHLDQLIQVFGPEQVYLEVQKNGLADQNKANEQIARIAREVGRPLVGTADVH"
BASE COUNT          448 a          802 c          844 g          398 t
ORIGIN      
        1 atccagcgcc gcgacccttg ccgccgagaa cgccacccgg gccgggcaga tcacttcgct
       61 caagggtgac atcgccggct ggcagaagca gatggaaaag cagcagcagg taagcgccga
      121 acaggcccag gtagaggtcg agcagaccct cggcggcccg tactcgatcc cgacctacat
      181 cgtgatgtgt gaatccggcg gtgattactc ggcggtcaac ccctcaagcg gggctggcgg
      241 ggcctaccag atcatgcctt ccacctggga ggcctacggt ggcaccggac tgccccagga
      301 tgcctcgaag gccgaacagg accgcatcgc cgccctgatc tgggccgact ccggctccag
      361 cccctggacc tgcggctgac ttaactgcaa gtcgggcggg gtcggacccg ccctccaagt
      421 tcaacccgcc cgatcagatc atcaaatatt tgatgatcgg gctccgccgg tggctatttc
      481 gatgagttcc gtggggcgga ggagggcgtg gaggtggctt cccttgatca gggcgaggcc
      541 gccctttttc atcttgatgt tggctccggt gaggcgggcc acctccatcg agaggaccgg
      601 ttcgtggccg acgatcaggg tgtcgccgcg gccggcggca agctcgaggc tgtcgtagtc
      661 gccgtagccg atcggctccg agacctcgac cttgattccc agcgaccggc aggcaagggt
      721 ggcggtgtca aaggcgcgga cccggggcga ggccaggcag gtatccacgg tcatgcccag
      781 cttcgccatc gccgcgccgg cggccagggc ctgggcctcg cccttttcgg tgaggcggcg
      841 ggcggcatct cccgaaccct ggtcgggttc ggcgtcaccg tgacgaagca ggtagatcat
      901 gggtcgagat tacccgccaa ctgcaactag aatcggccca tgaccgaggc cgccgcagtt
      961 cctgacttct ccaccttgct ggttagcgcc gaggggccga tcgggaggtt gaccctgaat
     1021 cgtcccgaaa agctcaacgc gctttccggt gaactgctga acgagctgat cctggcctcg
     1081 aaatggttca cggacagaac cggggtgaag gttgtcctgg tcagcggtgc cgggcgggcc
     1141 ttctgcgccg gggccgacct gacctccttc tcggccgccg gttcggaccc gagcgaggaa
     1201 gaacgccggg acggggcgga cctggggcgg aggatggccg acgccgtgac cgggatgcgg
     1261 ccgatgaccg tcgccgcgat ccaggggcac tgcgtcgggg gaggcctggt gctggccgcc
     1321 gcctgcgatt tgcgcattgc cgcggaggac acgcgctttt cgatcccgga agtggacctc
     1381 ggcatcccgc tcgcctgggg cggcatcccc cggctggtca gggagatcgg gccggccgca
     1441 accaaggaac tggtgctgac ctgccgcgag ttcgatgccc gggaagccag ggaactcggc
     1501 tttctcaacc ggacggttga cccggaccgg ctcgaaggcc aggcctggga actggcggag
     1561 aagctcgcct cccagccggc ctactcgctt gaactgacca agcggcacgt gaacgccgtg
     1621 gctgaagaat ccggttcgac ggccgatgcg atcaccgacg ccgacggcct gctcggagcc
     1681 ctcggcgacc ccgaatcgct ccggaagatg ggcgaatacc tggcccgtcg cggctgaacg
     1741 ccagcgggga tcgggccttc ccgcaagtta cgggaaaaag cgtcccgagc ggagcaaatc
     1801 gaccgggttt gcatcatgcg cacttgccat catggaccgg cgatgagttc ttcggcatcc
     1861 agccacggac aggtagtcca ccttcacgtt cacagcgagc attcggtgct ggatggtgcc
     1921 tgcaacatca aggaaatggc ggcgcgggca gctgaactgg agatgccggc tctggccctg
     1981 accgaccacg gcgtcatgaa cggcgccctc gacatgtaca aggcctgccg ggccaatgac
     2041 atcgagccga tcctcggcat cgaggcctat tacgtcgatg accggatcgc ggtgaaggaa
     2101 acggccggcc ggtacgaacg aaatcacctc accctgctgg ccgagaacaa caccggtttc
     2161 gccaacctgg ttgcgctttc ctcggagggc ttcctcgagg gctatcaccg cggcaaggcg
     2221 aacgtggaca tggaactgct cgaacggcac tccgagggag tcatcgccct gaccggctgc
     2281 ctgcaggccc ggttcgtgaa gcggctggtc gaggaccggc cggacgaggc ccgcgagcac
     2341 ctggaccagc tgattcaggt gttcggaccc gagcaggtct atctggaggt tcagaagaac
     2401 ggtctggccg accagaacaa ggcgaacgag cagatcgccc ggattgcccg ggaggtcggc
     2461 cggcccctgg tcggtaccgc cgacgtccat ta
//
LOCUS       DAOQFI010000257         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_22967_length_2485_cov_3.004527, whole genome shotgun sequence.
ACCESSION   DAOQFI010000257 DAOQFI010000000
VERSION     DAOQFI010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22967_length_2485_cov_3.004527"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="hemA"
                     /locus_tag="PLE93_05360"
     CDS             complement(<1..924)
                     /gene="hemA"
                     /locus_tag="PLE93_05360"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNH86510.1"
                     /translation="MSEVLALGLSHKTAPLELRERLALPEGRAARLMAELTDSGDASE
                     ATVISTCNRTEIYVFAHDPVVAESSILSALASQGGISPTELAGHLYSLRGDDAALHLF
                     RVTAGLDSMIIGESEVQGQVKRAHELALVEGVSGPILNRLFRGALAAGGRARNETGIS
                     RTGVSMASVAVEQATRAIGEVGDRRALIIGAGETSELVAGALRARGAESVFVANRHLE
                     KACDLTRKFGGTAAPLTELSEQVAEADLVVSATMSPHHLIDRELLEPVMKERQERPVF
                     LVDLAVPRDIDPRVRELPGVSLTDVDELQVAV"
     gene            complement(917..1138)
                     /locus_tag="PLE93_05365"
     CDS             complement(917..1138)
                     /locus_tag="PLE93_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86511.1"
                     /translation="MSDALPGDAGYVFVAYMVFLALLLIYLGILGAKFQRINRDIARL
                     GEEVESGREREPAGPAGSPESGSREPTSV"
     gene            complement(1139..1843)
                     /gene="ccsA"
                     /locus_tag="PLE93_05370"
     CDS             complement(1139..1843)
                     /gene="ccsA"
                     /locus_tag="PLE93_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNH86512.1"
                     /translation="MLLAGFGLVFFWVPMDAIEGFRQKIFYLHVPLAIVALCGFMAGG
                     VEGFRHLRSNDRRHDANSYVFIHISVVFGVATLITGMIWAKASWGHYWVWDEPTLVSF
                     LIVFLLYCTYYPFRYAIEDRDRQARYASVFAITAGAFVPLNFMAVRMAESLIHPRVFA
                     TSQGGLPGSMLFTFLVCLVGVALLWVTLVKLELLGKQTSGQVSKLRRALEAEGFGKSE
                     PTGTVGRRIAPNLEAR"
     gene            complement(1911..2429)
                     /locus_tag="PLE93_05375"
                     /pseudo
     CDS             complement(1911..2429)
                     /locus_tag="PLE93_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
BASE COUNT          482 a          788 c          837 g          378 t
ORIGIN      
        1 caccgccacc tgcagctcgt caacgtcggt caggcttacc ccggggagtt cccgcacccg
       61 cggatcaatg tcccgcggga ccgccaggtc gacgaggaag accggacgct cctggcgttc
      121 cttcatgacc ggttcgagca gttcgcgatc gatcaggtgg tggggtgaca tggtcgccga
      181 aacgaccagg tcggcctcgg ccacctgctc gctcaactcg gtcagcggtg cggccgtacc
      241 accgaacttc cgggttaggt cgcaggcctt ttcaaggtgc cggttggcga cgaagacgct
      301 ttcggccccg cgggcacgca gggcaccggc cacaagttcc gaggtttcgc cggcgccgat
      361 gatcagggcc cggcgatcac cgacctcccc gatcgcccgg gtcgcctgtt caaccgcgac
      421 cgaggccatc gagactccgg tccggctgat tccggtctcg ttcctggccc ggcccccggc
      481 ggcgagggca ccgcggaaga gccggttgag gatcgggccg gagacgcctt cgaccagtgc
      541 cagttcatga gcccgcttga cctggccctg gacctcggac tcgccgatga tcatcgagtc
      601 gagcccggcg gttacccgga agaggtggag cgccgcgtcg tcgccgcgca gcgagtagag
      661 gtggccggca agttcggtcg ggctgattcc gccctggctg gcaagggccg aaaggatgct
      721 ggactcggcg accaccgggt cgtgggcgaa gacgtagatc tcggtccggt tgcaggtcga
      781 gatgaccgtc gcttccgagg catccccgga atcggtcagc tcggccatca ggcgggccgc
      841 ccggccttcg ggcagggcga gcctttcgcg cagctccagc ggagccgtct tgtgtgaaag
      901 cccgagtgcg agaacctcag acactggttg gctctctgct gccgctttcc ggggaccctg
      961 ccggaccggc cggttcccgc tcgcggcccg attcgacctc ttcgccgagc cgggcgatgt
     1021 cacggttgat ccgctggaac ttggcgccga ggatgccgag gtagatcaag agcagggcca
     1081 ggaagaccat gtaggcgacg aagacgtagc cggcgtcacc gggtagggca tcagacattt
     1141 accgggcctc caggttgggt gcgatgcgac ggccgactgt cccggtcggc tcggacttgc
     1201 cgaatccttc ggcctcgagg gcgcgccgca gtttcgatac ctggccggaa gtctgtttgc
     1261 cgagcaattc gagtttgacc agggtgaccc agagcagggc cacaccgacc aggcagacca
     1321 ggaaggtgaa cagcatcgag ccgggcaggc cgccctggct ggtggcgaag acccgagggt
     1381 ggatcaggct ttcggccatc cgcaccgcca tgaagttgag cggcacgaag gcgcccgcgg
     1441 tgatcgcgaa gaccgaggcg tagcgagcct ggcggtcccg gtcttcgatc gcgtagcgga
     1501 acgggtagta ggtgcagtag aggaggaaga cgatcaggaa gctgaccagg gtcggctcgt
     1561 cccagaccca gtaatggccc cacgaggcct tcgcccagat cattccggtg atcagcgtcg
     1621 cgaccccgaa aacgaccgag atgtgaatga agacatagga gttggcgtca tgccgccggt
     1681 cgttcgaccg cagatgacgg aacccctcga cgccgccggc catgaagccg cagagcgcga
     1741 cgatcgccag cggcacatgc aggtagaaga tcttctggcg gaaaccttcg atcgcgtcca
     1801 tcggcaccca gaaaaagacc agcccgaagc cggcgagcag cacgagcgcc gtggctatcg
     1861 aaagttgtcc tagaccgcgt cctgacatat ccgttccgcc cggggtcgat tcagtcctcg
     1921 aggaggaagt cgaaaacggc ataaccgaca aggcagaaga tcccatcgta tagaccgagg
     1981 accagcagcc atgttccgaa cttgtcatag ctcggcccgc ccgcgtccag caagtgggtg
     2041 gtggctccgg tcgcggcgat catcagcggc accaccagag gcaggagaag caacggcacg
     2101 agcaggtcgc gggccctcga attgaccgcc atcgacgaga tcagggtccc gagcgcggca
     2161 aaaccgaggt tggtgagcag gagcacgacg atcatcgggg caagcccgct ccattcctga
     2221 aggaagaaga ccgcgaagac cggaaccacg atcacttcca gcaccgccag gtagaggaag
     2281 agcacggcag ccttggcggc gaagagcgcc gggcggtcca ccggggcaag ccggaatgcg
     2341 tcgaagccgc cctcttcccg ctccggcagg aagatccggt tgatcccgag caccgcggcg
     2401 aagagcaggg tcgtccagat gaccccggag gcgaggctgc ccgaaagggc agcctcgact
     2461 gttttttaag tgttacaatt taaga
//
LOCUS       DAOQFI010000258         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_23055_length_2480_cov_3.692784, whole genome shotgun sequence.
ACCESSION   DAOQFI010000258 DAOQFI010000000
VERSION     DAOQFI010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23055_length_2480_cov_3.692784"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1379
                     /gene="nhaA"
                     /locus_tag="PLE93_05380"
     CDS             180..1379
                     /gene="nhaA"
                     /locus_tag="PLE93_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNH86513.1"
                     /translation="MKRILRQRVIDPLTDFLDDEVLGGVILLGAAIIAVVWANSGWSD
                     GYFEFWSSYIKVDIGPVDISLDLQHWINDLLMAVFFFIVGIEIKRELVSGELQNRSDA
                     VVPVLAALGGVAMPAIIFTLIAGGGPAADGWAIPAATDIAFAVGILALLGNRVSNGVR
                     VFLLTIAIVDDIVAILIIALFYGDGLSLGWAAIAGAGVLVVVGFQKFGVWRIWPYVPL
                     AALIWIGTYESGIHATIAGVVLGLLVPVRPFRGRDINTKLEHALHPVSAFVVVPLFAL
                     ANAGIDLRGGVLGDALGSELTWAVIAGLVVGKILGISVTTLAALRFGSGTLPEGMRRA
                     QVWGVGALGGIGFTVSLFITDLAFTDPLLLDAAKVGIFLGSILSGLLGTILLLRRRGA
                     GPPAETD"
     gene            complement(1381..2421)
                     /locus_tag="PLE93_05385"
     CDS             complement(1381..2421)
                     /locus_tag="PLE93_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH86514.1"
                     /translation="MSESQHVASLTEATALFEGDPGSLRVLTSAELPVLRGISIKRLL
                     LAPGSIREPHWHANASELTYCVKGDLLVSVLDDADGFSSFTISAGEMFYVASGSLHHI
                     ENLGSGEAELILAFSHERPEDFSMHAAFGAMSDAVLGNTYDLPAADFAVISRNTEPVY
                     IVSREGRPSVPEHSSFDDPHKFAIEGQAAPIDSPAGSAKLARKQFWPIMEDLSMYSLR
                     VTPDGMREPHWHPVTAELGYVVKGEARMTVMDPDGSTDTYGLGPGDVYFIPPAYPHHI
                     EDTGQDDFHFLVFFDRHTPKDIGYRASASAISREVLAATLGIPETMLPKIPFTPEDPL
                     IVKRANPVDPPG"
BASE COUNT          400 a          772 c          843 g          465 t
ORIGIN      
        1 ccgagatcat gaactggccg ccgtggagtt cgggttcgga ggtgatgcgc ctcaccccgt
       61 cgaggcaggt gagcctctcg actagttggt cgcgctcgct ctggagcacg tccacccgca
      121 gcctcagcat ggcaacccat cttccacttc cggcaaagta cccatgcagc aggacggata
      181 tgaagcgcat tctcagacaa cgagtgatcg acccgctgac cgacttcctc gacgacgagg
      241 tgctcggtgg agtcatcctg ctgggcgcgg cgatcatcgc cgtggtctgg gccaattcgg
      301 gctggagcga cgggtacttc gagttctggt ccagttacat caaggtcgac atcggcccgg
      361 ttgacatcag cctggatctt cagcactgga tcaacgacct gctgatggcg gtcttcttct
      421 tcatcgtcgg gatcgagata aagcgggagc tcgtttccgg cgagctccag aaccgcagtg
      481 acgcggtcgt gccggtactg gcggcgctcg gcggggtggc aatgccggcg atcatcttca
      541 ccctgatcgc cggcggcggc ccggcggcgg acggctgggc gatcccggcg gcgaccgaca
      601 tcgccttcgc ggtcgggatc ctcgccttgc tcggaaaccg ggtttcgaac ggggtccggg
      661 ttttcctgct gaccattgcg atcgtcgatg acatcgtcgc gatcctgatc atcgccctct
      721 tttacggaga cgggctttcg ctcggctggg cggcgattgc cggggccggg gtcctggtcg
      781 tggtcggctt ccagaagttc ggcgtctggc gaatctggcc gtacgtgccg ctggccgccc
      841 tgatctggat cggcacctat gagtcgggaa tccacgcgac gatcgccggc gtcgtgctgg
      901 ggctgctggt gccggtcagg cctttccgcg gcagggacat caacacaaag ctcgagcatg
      961 cccttcaccc ggtcagcgcc ttcgtggtgg tgccgctctt cgccctcgcc aacgcgggca
     1021 tcgacctgag gggcggggtt ctgggtgatg cgctcggcag cgagctgacc tgggcggtga
     1081 tcgccggcct ggtggtcggc aagatcctcg ggatctcggt cacgaccctg gctgccttgc
     1141 gtttcggttc cggcaccctg ccggaaggca tgcggagagc acaggtctgg ggcgtgggcg
     1201 cgctcggtgg catcggcttc accgtttcgc tgttcatcac cgatctggcc tttacggatc
     1261 cactcctgct cgacgcggcc aaggtcggca tcttcctcgg ctcgatcctc agcggcctcc
     1321 tgggcacgat tcttcttctg cgccggcgcg gggccgggcc cccggccgaa acggactgac
     1381 tcagccgggc gggtcgaccg gattggcccg cttcacgatc agcggatctt ccggggtgaa
     1441 ggggatcttc ggcagcatgg tctcggggat ccccagcgtc gcggccagca cctcccgcga
     1501 aatggccgaa gccgaagccc ggtagccgat gtccttcggg gtgtgccggt caaagaaaac
     1561 gaggaagtgg aagtcgtcct gaccggtgtc ctcgatgtgg tgagggtagg cgggagggat
     1621 gaagtagaca tcgccgggcc cgagcccgta ggtatcggtc gaaccgtccg gatccatcac
     1681 cgtcatcctg gcctcgccct tgaccacgta ccccagctcg gcggtgaccg gatgccaatg
     1741 cggttccctc attccgtccg gggtgacccg cagcgagtac atcgagaggt cctccatgat
     1801 cggccagaac tgcttgcggg ccagcttcgc cgagccggcc ggggagtcga tcggggcagc
     1861 ctgcccttcg atcgcgaact tgtgtggatc gtcgaatgag gaatgctccg gaaccgacgg
     1921 ccggccctcg cggctgacga tgtaaaccgg ctcggtattg cgggaaatca cggcgaaatc
     1981 cgcggcaggc aggtcgtagg tgttgccgag tacggcgtca ctcatcgctc cgaaggcggc
     2041 gtgcatcgaa aagtcttcgg gtcgctcgtg actgaacgcc aggatcaact cggcctcgcc
     2101 ggaacccagg ttctcgatgt ggtgcaggct gccgctggcc acgtagaaca tctccccggc
     2161 cgaaatcgtg aacgaggaga agccgtcggc gtcgtcgagg accgagacca gcaggtctcc
     2221 cttgacgcag taggtcaatt cgctggcgtt ggcgtgccag tgcggttccc ggatcgaacc
     2281 cggggcgagg agcagccgct tgatcgagat cccgcgtaaa accggcagct cggccgaagt
     2341 cagaacccgg agcgagcccg ggtcgccctc gaacagggcc gtggcttcgg tcagggaagc
     2401 aacatgctga ctctcgctca tgtccggcga ccctagcagc ggggatcggg ggtaggatcg
     2461 aaggatgttt cggggagagg
//
LOCUS       DAOQFI010000259         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_23169_length_2474_cov_3.663911, whole genome shotgun sequence.
ACCESSION   DAOQFI010000259 DAOQFI010000000
VERSION     DAOQFI010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23169_length_2474_cov_3.663911"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLE93_05390"
     CDS             complement(<1..966)
                     /locus_tag="PLE93_05390"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNH86515.1"
                     /translation="MARTVILSTARTPFGKMGGGLATVNAAELGGVAIAGALERAGVA
                     PDQVQQVSYGQVIQAGQGQIPSRQAQINGGIPKEVPSETVNKVCASGMRAAGLIDQAI
                     RAGDIEVGVAGGMESMSLAPYLLPGARSGFRMGDVKAVDSMTHDGLTNPFTHLQMIAE
                     ASTVSNEVGISREEMDAWSARSHELAQKAKEAGVLAEEIVPVTVKGRKGDTVVDEDEA
                     IRPGTTAESLSGLRAVGGEDATHTAGNAPGVNDGAGALVLASEEWAEANGKTPMATIV
                     GYGQIGDEYACLAKTPALAAKAALDRLGKTPADVDLWEVNEAFASV"
     gene            1173..>2474
                     /locus_tag="PLE93_05395"
     CDS             1173..>2474
                     /locus_tag="PLE93_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86516.1"
                     /translation="MAGEVSAGPVSTYTTGPVADINPGAGSGISNSVDPEFTAAGNRV
                     FFVADDGVNGKEVWVSDGRPGGSTQMAKDINPAPGVGSNPRDLVTYQGEVYFSADDGA
                     SGRELWKSDGTEAGTVLLKDIQPGTDPSLPGRPVEAGGKLFFAANEGPGPTFRGNELW
                     VTDGTGPGTVMVRDIEADGCLGGSSPRELTEVGGLLYFVANFSSPGCSDSSLGELWKS
                     DGTEAGTERVSDLNGPGDASDPFITSITALNGLAVFAADLPATGQELFASDGTDFGTN
                     LVMDIKPGSGNGVMSRLAAFNGWVYFQGNDSTNRELWRSNVTSTTELFKDVDPAGQSQ
                     PDGFTVANGALWFDAFVTGLSDEPWVSDGTPGGTNLVKDINTSGNSYPEDFTGLGDRT
                     FFLASDTSSTPFTMTNKQLWQSDGTATGTFARSSIAPGDPIE"
BASE COUNT          435 a          792 c          832 g          415 t
ORIGIN      
        1 gaccgaagcg aaggcctcgt tcacctccca gaggtcgacg tcggccgggg tcttgccgag
       61 cctgtccagc gccgcctttg cggcgagagc cggggtcttg gcgaggcagg cgtactcgtc
      121 cccgatctgg ccgtaaccga cgatggtcgc catcggggtc ttgccgttgg cctcggccca
      181 ctcctcggag gccagcacga gcgccccggc gccgtcgttc acgcccgggg cgttgccggc
      241 ggtgtgggtg gcgtcttcgc cgccgaccgc acgcaggccg gagaggctct cggcggtggt
      301 accggggcgg atcgcctcgt cttcgtcgac cacggtgtcg cccttgcggc ccttgaccgt
      361 gaccggcacg atctcctcgg ccagcactcc ggcttccttc gccttctggg ccagctcatg
      421 cgagcgggcc gaccatgcgt ccatctcctc gcggctgatg ccgacttcat tcgaaacggt
      481 cgaggcctcg gcaatcatct gcaggtgagt gaagggattg gtcaggccgt catgggtcat
      541 cgagtcgacc gccttgacgt cacccatgcg gaaaccggac cgggcaccgg gcagaaggta
      601 cggcgcgagc gacatcgatt ccatgccgcc ggcgacgcca acctcgatgt caccggcgcg
      661 aatcgcctgg tcgatcaggc cggcagcccg catgccggag gcacagacct tgttcacggt
      721 ctcggaaggg acttccttcg ggatgccacc gttgatctgc gcctggcggg aagggatctg
      781 gccctggccg gcctgaatca cctggccgta gctgacctgc tggacctggt ccggggcgac
      841 tccggcgcgc tcgagggcgc cggcgatcgc cacaccgccg agttcggccg cattcacggt
      901 ggcgagtcca ccgcccatct tgccgaaggg ggtgcgggca gtgctcagga tgacggtgcg
      961 ggccatggtg cctttcaggt ctcgagggga aatcggaaaa cttcggacga aacgaagcgg
     1021 aatatggaag ataccgggtg ccccttaccg ggggcctctg cttcggttag gcttgactca
     1081 atccaggcga acgggggttt ctggcagtgg ggagtttcag actgaggagt gtctcgcttg
     1141 tctttgtgct gtttgcgtgg ggatccttgc tgatggctgg cgaggtcagc gcgggaccgg
     1201 tctcgaccta cacgaccggg ccggtggcgg acataaaccc gggtgccggc agcggtatct
     1261 cgaactcggt cgacccggaa ttcaccgcgg ccggaaaccg ggtcttcttc gtggccgatg
     1321 acggggtgaa cgggaaagaa gtctgggtca gcgacggcag gccgggcgga agcacccaga
     1381 tggccaagga cataaacccg gcccccgggg tcggcagcaa ccctcgggat ctcgtcacgt
     1441 accagggcga ggtctacttc tcggcggacg acggagcgag cggccgggaa ctctggaaat
     1501 cggacgggac cgaggccgga acggtgctgc tcaaggacat ccagcccggt accgatccat
     1561 cgttgccggg caggccggtc gaagccggcg gcaaactctt tttcgccgcc aacgaaggcc
     1621 ccggtccgac cttcaggggg aacgaactct gggtaaccga tggcacgggc ccgggaactg
     1681 tgatggtcag ggacatcgag gccgacggct gtctgggtgg gtcgagcccg agagaactga
     1741 ccgaagtcgg cgggcttctc tactttgtcg ccaacttctc gagtccgggc tgcagcgatt
     1801 ccagcctggg cgaactctgg aagagcgacg gcaccgaggc cggaaccgaa cgggtctcgg
     1861 acctgaacgg gccgggtgac gcctcggacc cgttcataac ctcgataacc gcactgaacg
     1921 gactggcggt attcgccgct gatctgccgg caaccggcca ggagcttttc gcaagcgacg
     1981 gcaccgactt cggcaccaac ctggtcatgg acatcaaacc cggttcgggg aatggcgtga
     2041 tgagtcgcct tgcggccttc aacggctggg tctacttcca gggaaacgac tcgaccaacc
     2101 gcgaactctg gcgatcaaac gtgaccagta caaccgaact tttcaaggat gtcgacccgg
     2161 ccggtcagag ccagcccgac gggttcaccg tggcgaacgg ggcactgtgg tttgacgcct
     2221 tcgtcaccgg actctccgac gagccctggg taagcgacgg cacccccggc ggtacgaatc
     2281 tggtcaagga catcaacacc tcgggcaact cgtatccgga ggacttcaca gggctggggg
     2341 accggacctt cttcctggcc agcgacacgt cgagcacgcc cttcaccatg accaacaaac
     2401 agctctggca gtcggatggc accgccaccg ggaccttcgc cagaagctcc atcgctcccg
     2461 gggacccgat cgag
//
LOCUS       DAOQFI010000260         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_23299_length_2467_cov_4.055970, whole genome shotgun sequence.
ACCESSION   DAOQFI010000260 DAOQFI010000000
VERSION     DAOQFI010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23299_length_2467_cov_4.055970"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(437..>2467)
                     /locus_tag="PLE93_05400"
     CDS             complement(437..>2467)
                     /locus_tag="PLE93_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNH86517.1"
                     /translation="PPVQTNDSDAKTKDASGASGNHSGPYEDEDDAKATLKFPIADLK
                     IEKSAPAQVNVYDEFDYTLKITNKGPDTATQVTVSDPLPAGLVFVSSADCNAVMTCSL
                     GTMAANTSKTVTIRVKTTYLVGGTTVHNVATVTGHEWEPTPEDNTDDADTFVVGLPDL
                     KITKTAAPTNARPGDVVTYTLKAENVGNAVAHNVLITDPVPVGMTFVSADSPCTESGG
                     TVNCAISDLNPGQVKTYTVKATVDPWGDADPSADHLLDVQKVETQIDLNPGETKTVQA
                     VCPSGYFASDGSVRIDHVDQGTGDWTAPEVLESRASSLDTWQGTVHNSAAGRAQAKIF
                     AVCISKQTVADGGHQHDLIVSAPITVTNLTPAGKNEATLQCGPGQIAIQSGFNSSVPA
                     DLVYSEPEGNGWKFILDSKVDGQATFSIRCMTRQVSVVDGHTHDLQFQHIVTEFTFPA
                     GQVSEAQLTCADGYKGIVADMDLDDGLVSLGNDPRPVTRAFKIYNPTDHDLTGRLSLL
                     CLGLRTAGEHVPPKVLQNTAYISTTSFEEVTTNNHSTATVTAEDTDNYEPVEPTDPVK
                     PTPNNPVAKTIVGKGVSYSATGVTFTLKCSGACGGFAKLSSLKKVKVRGKSYRRGTVL
                     AKKQYFIGKAGSKRIKLTLTRAGKGILKSGKVKKASLRINGGTSKVVKIGRR"
BASE COUNT          443 a          755 c          752 g          517 t
ORIGIN      
        1 attgtctccc cagcaagctg ccctcccgga gttcaacacc gcgcaggcgt ggctcggacc
       61 tacagcgact gaagttgcct ctagcggttg acttgcctgg ccggccgatg gcacaaccag
      121 tccgcctgcc aacgcgaaga tggccgctac acagaacgca gccagaaaag tcagagcgtt
      181 cgagctacag gttgctttgg ccatcgaact gctgctgttc tcccggatat tcatttgacg
      241 gctccttgtt ctttgggtgt tcgacatgac actgtgaaca ggacggtcaa gggttcgcgg
      301 gtcttccgga ttccccgctc atgtgacctg actcacgcaa gctgtctggc gctcatcgac
      361 aggcgccggc atagcaacaa aaagccccgg tctttcgacc ggggctcttt tctccctggc
      421 agtgagtgga tcggggctag cgccgcccga tcttcactac cttgctggtc cctccattaa
      481 tcctgagtga agccttcttg accttgcctg atttcaggat ccccttaccg gcccgggtaa
      541 gcgtcagctt gatgcgctta ctgcctgcct tgccgatgaa gtactgcttc ttcgccagga
      601 cggtgccccg gcggtaactc ttgcccctga ccttgacctt cttaagggat gacagcttcg
      661 cgaagccgcc acaggcaccc gagcacttca gcgtgaaggt aacgcccgtg gccgagtagc
      721 tgacgccctt gccgacgatc gtcttcgcga ccgggttgtt cggggtcggc ttgaccgggt
      781 cggtcggttc caccggctcg tagttgtcgg tgtcctcggc ggtcacagtc gcggtcgagt
      841 ggttgttcgt cgtcacttcc tcgaaagaag tcgtcgagat gtaggcggtg ttctgaagca
      901 ccttcggcgg cacatgctca cccgcggtcc tgagaccgag gcaaagcagg ctgagccttc
      961 cggtcaggtc atgatcggtc gggttgtaga tcttgaacgc acgggtcacc ggacgcggat
     1021 cgttgcccaa cgaaaccagg ccgtcatcca ggtccatgtc agcgacgatg cccttgtaac
     1081 cgtcggcgca ggtgagctgg gcctcagaga cctgaccggc cgggaaggtg aactccgtga
     1141 cgatgtgctg gaactgaagg tcatgggtat gaccatcgac cacactgacc tgacgggtca
     1201 tgcagcgaat cgagaaagtc gcctggccgt caaccttgct gtcaaggatg aacttccagc
     1261 cattgccctc cggctcgctg taaaccagat cggccggaac gctcgagttg aaacctgact
     1321 gaatcgcgat ctggcccgga ccgcactgaa gcgtcgcctc gttcttgccg gccggcgtca
     1381 ggttggtcac cgtgatcggg gccgagacga tcagatcatg ctggtggcca ccatcggcca
     1441 cagtctgctt cgaaatgcag accgcgaaga tcttcgcctg ggcgcgacca gccgccgagt
     1501 tgtggacggt gccctgccag gtatccagcg aggaagcacg cgactcgaga acttcggggg
     1561 cggtccagtc accggtgccc tgatcaacat ggtcgatccg caccgaaccg tcagaagcga
     1621 agtaaccgga cgggcaaacc gcctggaccg tcttcgtctc acccggattc aggtcgatct
     1681 gggtctccac cttctggaca tccagcaggt gatcggctga aggatcggcg tcaccccagg
     1741 gatcgacagt ggccttgacc gtgtaggtct tgacctgacc cggattgaga tcactgatcg
     1801 cgcaattcac cgtgccaccc gactcggtac agggcgaatc ggcactgaca aaggtcatgc
     1861 caaccggcac cggatcggtg atcaacacat tgtgggcgac cgcgttaccg acgttctcgg
     1921 ccttcagggt gtaagtcacg acatcacccg gacgcgcatt cgtcggcgcc gcagtcttgg
     1981 tgatcttcag gtcgggaaga ccgaccacga aggtgtcggc gtcatcggtg ttgtcttccg
     2041 gggtcggttc ccattcgtga ccggtcacgg tcgcgacgtt gtgaacagtg gtgccaccga
     2101 ccaggtaggt ggtcttgacc cggatcgtga ccgtcttcga ggtgttcgcc gccatggtgc
     2161 ccagcgagca ggtcatgact gcgttgcagt cggccgaaga gacgaacacc agaccggccg
     2221 gcagcggatc gctgaccgtc acctgggtcg cagtgtcggg acccttgttg gtgatcttca
     2281 gggtgtagtc gaactcgtcg tatacgttga cctgggccgg cgccgacttc tcgatcttca
     2341 ggtcggcgat cgggaacttg agcgtcgcct tggcgtcgtc ctcgtcctcg taaggaccgc
     2401 tgtggttgcc actggcaccg ctcgcgtcct tggtcttcgc gtccgagtca ttggtctgga
     2461 ccggcgg
//
LOCUS       DAOQFI010000261         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_23430_length_2458_cov_3.427799, whole genome shotgun sequence.
ACCESSION   DAOQFI010000261 DAOQFI010000000
VERSION     DAOQFI010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23430_length_2458_cov_3.427799"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..289)
                     /gene="rpmA"
                     /locus_tag="PLE93_05405"
     CDS             complement(35..289)
                     /gene="rpmA"
                     /locus_tag="PLE93_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNH86518.1"
                     /translation="MAHKKGLGSSRNGRDSNPKMLGVKVFAGETVSGGEIIVRQRGTK
                     FWPGEGTGLGRDHTIFATRPGTVEFKPGRRGRTISVAPAE"
     gene            complement(279..821)
                     /gene="rplU"
                     /locus_tag="PLE93_05410"
     CDS             complement(279..821)
                     /gene="rplU"
                     /locus_tag="PLE93_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNH86519.1"
                     /translation="MYAVVESGGKQYRVEKGTVLVVDRLKAEQGDKVALKPVMFKDKE
                     VIVDAKGLEKIKIEATVTEHLRGDKIKVFKYKPKKGYSRRAGHRAELTKLEVTSLGGG
                     AKKAAPKKAAADKAEEKPKAEAKAETAEKKAPAKKPAAKKPAAKAETKTETKAAAKKP
                     AAKKPAAKKPAAKKEDTDGA"
     gene            complement(895..>2458)
                     /locus_tag="PLE93_05415"
     CDS             complement(895..>2458)
                     /locus_tag="PLE93_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNH86520.1"
                     /translation="RLRRMVSRTYDGPGGLIVRTAAHGAKKADFTREIAYLHRLDAVL
                     EKRREGAKAPSLVFQEQDLPVRVLRDVFLNEFDKAIIDDQKQFDRVTSFFQRTAPELV
                     EGVEHYKGKKPLFEKWEIERAIQSTLGRRIDLPSGGYLIIDYTEALTVIDVNSGSFTG
                     RGKGGLEETITKVNTEAASEVVRQLRLRDIGGIIVIDFIDMAKGKNRDKVLGTLREAL
                     DADKSKSYLVEISPLGLVEMTRQNITDGVREILTVQCPTCEGEGVVLSAETVALEGLR
                     KMKEIAADHAGDEAFLVRVNPKVAALLNDSDSGLLELEEETGKQFHFEGGEALAIDNF
                     ELIESGEKSSIEERALPFRPGEEVFVKIEEPHMYNADDAIARIDSYIISITGAANAVG
                     ERKMVRIQSVERASAVAELLPEEAQPKETKKAAPRKKNGSGQNAGRSENGKASASGRG
                     PKQETGNRGNDRQGRSGQGSKNGSDPKPKAESAVKKDAEGEKEDKPVKSGPFGLRRGR
                     RRRGKKKEDAGS"
BASE COUNT          320 a          816 c          762 g          560 t
ORIGIN      
        1 cgcggagcgg gcctttccga atcctgtttc ccggttactc ggccggagcg accgagatcg
       61 tcctgccgcg gcggccgggc ttgaactcga ccgtgccggg ccgggtggcg aaaatggtgt
      121 ggtcacggcc gaggccggtg ccctcgccgg gccagaactt cgtgccgcgc tggcggacga
      181 tgatctcgcc gccggaaacg gtttctccgg cgaagacctt caccccgagc atcttcgggt
      241 tcgagtcacg accgttccgt gaggatccga gtcctttctt atgcgccatc ggtgtcctcc
      301 ttcttggcgg ccggcttctt ggccgcaggc tttttggcgg ccggcttctt cgcggcagcc
      361 ttggtttcag tcttggtttc ggccttggcc gccggcttct tcgcggccgg cttctttgcc
      421 ggggccttct tctcggcggt ttccgccttg gcttcggcct tcggcttctc ctccgccttg
      481 tcggcagcag ccttcttcgg ggcggccttc ttcgcgccgc cacccaggga agtcacctcg
      541 agcttcgtca actcggcccg gtgaccggcc cggcggctgt agcccttctt cggcttgtac
      601 ttgaagacct tgatcttgtc gccgcgcaga tgctcggtga cggtcgcctc gatcttgatc
      661 ttctcgaggc ctttcgcgtc aacgatgacc tccttgtcct tgaacatgac cggcttcagg
      721 gcgaccttgt caccctgctc ggccttcagg cgatcaacga cgagcaccgt gcccttttcg
      781 acccggtact gcttgccacc actttcaact accgcgtaca tgtcttgaac tcaaatcctt
      841 gtctggtctt cctgaagctg agtcttctca gcctgaaatc ggcgggaccg aggatcaact
      901 accggcgtct tccttcttct tgccacgccg acgtcgtccg cgcctcaggc cgaacgggcc
      961 ggattttacc ggtttgtcct ctttttcacc ttctgcgtcc tttttcaccg ctgattcggc
     1021 tttgggtttc gggtcggaac cgttcttcga gccctgaccg gacctgccct ggcgatcgtt
     1081 accgcgattc ccggtctcct gtttcgggcc gcgacccgac gcggaagcct tgccgttttc
     1141 gctcctgccg gcattctggc ccgacccgtt cttctttcga ggggcggcct tcttcgtctc
     1201 cttgggctgg gcttcctccg gcagcagctc ggccaccgcg gaggcccgct cgaccgactg
     1261 gatgcggacc atcttgcgct caccgaccgc attcgcggca ccggtgatgc tgatgatgta
     1321 ggagtcgatc cgggcgatcg cgtcatccgc gttgtacatg tgcggctctt cgatcttgac
     1381 gaacacttcc tcgcccggcc ggaagggcag ggccctttcc tcgatcgaag acttctcgcc
     1441 ggactcgatc agttcgaagt tgtcgatcgc cagggcctcg ccgccctcga agtggaactg
     1501 cttgccggtt tcttcctcga gctcgagcag gccggagtcg gaatcgttga gcagcgcggc
     1561 gaccttcggg ttgacccgga ccaggaaggc ctcgtcaccg gcgtggtcgg ccgcgatctc
     1621 cttcatcttg cggagacctt ccagggcgac cgtctcggcc gagagcacca cgccctcgcc
     1681 ctcgcaggtc gggcactgga cggtgaggat ctcgcgcacc ccgtcggtga tgttctggcg
     1741 ggtcatctcg accaggccga gcggcgaaat ctcgaccagg tagctcttcg acttgtcggc
     1801 gtcgagcgct tcccgcaggg tgccgagcac cttgtcgcgg ttcttgccct tcgccatgtc
     1861 gatgaagtcg atgacgatga tgccgccgat gtcgcgcagc ctgagctgcc ggacgacttc
     1921 ggaggccgcc tcggtgttga ccttggtgat cgtctcctcg aggccgccct ttccgcggcc
     1981 ggtgaaggaa ccggagttca cgtcgatcac ggtcagcgcc tcggtgtagt cgatgatcag
     2041 gtagccgccg gagggcaggt cgatacgacg gcccagggtc gactggatcg ccctttcgat
     2101 ctcccatttc tcgaagagcg gcttcttgcc cttgtaatgc tcgacccctt cgaccagctc
     2161 cggcgcggtg cgctggaaga agctggttac ccggtcgaac tgtttctggt catcgatgat
     2221 cgccttgtcg aactcgttga ggaagacgtc gcggagcacg cggaccggca ggtcctgctc
     2281 ctggaagacg agggatggcg ccttggcgcc ctcgcggcgc ttctcgagca cggcgtcgag
     2341 gcgatgcagg taggcgatct cgcgggtgaa gtcggccttt ttggcgccgt gggccgcggt
     2401 gcggacgatc aggccgcccg ggccgtcata ggttcggctg accatgcggc gcaggcgg
//
LOCUS       DAOQFI010000262         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_23724_length_2442_cov_2.529954, whole genome shotgun sequence.
ACCESSION   DAOQFI010000262 DAOQFI010000000
VERSION     DAOQFI010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23724_length_2442_cov_2.529954"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PLE93_05420"
     CDS             <1..1104
                     /locus_tag="PLE93_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNH86521.1"
                     /translation="EGERSGPAWEDYIRESLLPMIQSRFSIRPERRYHAIAGFSMGGY
                     GTWLTASQLPGFFGTAVPLSAFASIRTFESTLAFATAAGGTPYAQVYGDPTGFYAEGH
                     DPIALGPNLAFTNLDVFTGDGEPDPEVRPQNDPSNPGYVTDGDGVSLYLESVLKTQND
                     AAVAAMEASGNKNVDYTVHKGSHDWEFWRPDLKAAIAKGLFRPAPARPANWSYRTAAS
                     EGQAWDIYFNFSPAPTAVTTFKRTGNRLKVTGEGEMTIFDGNGCSFSADLPFTRTLSE
                     KPCRSLKLQAKGSGLRKGRTGTITVTVKGMNYTGVTKPVDAVKVEAGGASAITAYNGK
                     ATLKVKPKQSGKLTVKVAKDGFRPASAKLNVRK"
     gene            complement(1118..1753)
                     /locus_tag="PLE93_05425"
     CDS             complement(1118..1753)
                     /locus_tag="PLE93_05425"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNH86522.1"
                     /translation="MPEEIRDPNRLVTTLEPAELARIEEEVQEIRACVPAWVWDGRSL
                     PVPIDLIAREIYNLRVRVVSEEAMREAIGEDPDGHYGLSGLLICADGEIWVSKGELDY
                     DWGSRRHRFTVGHELGHFVMHQSREPRIFCREIENGESETGEVDQNDPVPRPVPEAEA
                     NTFSAAMLMPIDQVRKRLSSNPADPVKDLQDYFGVNRKPALRRVETARILG"
     gene            complement(1776..>2442)
                     /locus_tag="PLE93_05430"
     CDS             complement(1776..>2442)
                     /locus_tag="PLE93_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86523.1"
                     /translation="NDFEETPDPVIHAFDEYVAEFRKGHGDPSPFLERFEGSERKSLE
                     TLIESFVATGPESSPDPGDPRFNAVFEKVLDEVSAPAGGLSEHLALLRQKAGKTQAAV
                     VAALAKTFEATPQEQEKIDEYYHRLEWGSLPASGLDGKLYSALAAILGVEPESLKEAA
                     GSLGSRYGASSGPVFARSLDQVDLAEDASIVPGVIGQAVPDSESRSEPSRPDRIDELF
                     TGG"
BASE COUNT          464 a          818 c          739 g          421 t
ORIGIN      
        1 gaaggcgagc gcagtggccc ggcctgggag gactacatcc gcgagtccct gctgccgatg
       61 atccagagca ggttcagcat ccggcccgag cgccgctacc acgcgatcgc cggattctcg
      121 atgggcggct acggcacctg gctgaccgcc tcgcagctgc cgggattctt cggcacggcg
      181 gtgccgctat cggccttcgc ctcgatccgc acctttgaat cgaccctcgc cttcgcgacg
      241 gcggccggcg gcacccctta cgcccaggtg tacggcgacc cgaccggttt ctacgcggaa
      301 gggcatgatc cgatcgcgct tggaccgaac ctcgccttca ccaatctcga cgtgttcacc
      361 ggcgacggtg aacccgaccc ggaggtccgc ccccagaacg atccgtccaa ccctggctac
      421 gtgaccgatg gcgacggggt cagcctctac ctcgaaagtg tgctgaagac gcaaaacgat
      481 gcggccgtgg cggccatgga ggcatccggg aacaagaacg tcgactacac cgtccacaag
      541 ggcagccacg actgggagtt ctggcggccc gacctgaaag cggcgatcgc caagggcctg
      601 ttccgccccg cccccgcccg gccagccaac tggtcctaca gaaccgccgc ttccgaaggc
      661 caggcctggg acatctactt caacttcagc ccggccccga ccgcagtcac caccttcaag
      721 cggaccggaa acaggctcaa ggtgaccggc gagggcgaaa tgacgatctt cgacggcaac
      781 ggctgctcgt tctcggctga tctgcccttc acccgcaccc tgtcggaaaa gccctgccgc
      841 agcctgaagc ttcaggcgaa gggcagcgga ctgcgcaagg gcaggaccgg cacgatcacc
      901 gtgaccgtca agggcatgaa ctacaccgga gtgacgaagc cggtcgacgc ggtcaaggtc
      961 gaagccggcg gcgcctcggc catcaccgcc tacaacggca aggcaaccct gaaggtcaag
     1021 ccgaagcagt ccggcaagct caccgtcaag gtggcgaaag acggattccg gccggcctcc
     1081 gcaaagctga acgtccggaa gtagccgccc ggttcgttca gccgagaatc cgcgccgttt
     1141 cgacccgtcg aagcgccggc ttccggttga cgccgaaata gtcctggaga tccttgaccg
     1201 ggtcggccgg gttggaggaa agtctcttcc gaacctggtc gatcggcatc aacatcgccg
     1261 ccgagaaagt gttcgcctcg gcctccggca ccggcctcgg gaccgggtcg ttctggtcga
     1321 cctcaccggt ttcactctcg ccgttttcga tctcgcgaca aaagatcctg ggctcccggc
     1381 tctggtgcat cacgaagtgg ccgagttcgt ggccaaccgt gaatcggtgc cgtcgcgaac
     1441 cccagtcgta atcgagttcg cccttgctga cccagatttc accgtcagcg caaatcaaca
     1501 ggccggatag cccgtagtga ccgtcgggat cctcaccgat cgcttcgcgc atcgcttcct
     1561 cactgaccac gcgcacccgg aggttgtaga tctcgcgggc gatcaggtcg attggcaccg
     1621 gcaggctgcg gccatcccag acccatgcgg gaacgcaggc cctgatttcc tgaacctctt
     1681 cctctatccg ggcaagctcg gcaggctcga gcgtggttac gagcctgttc gggtctctga
     1741 tctcctccgg cacgcaggcg attgtagaag gcgggtcagc cgccagtgaa aagctcgtcg
     1801 attcgatctg gtctggacgg ttccgaccgg gattcagaat ccggcacggc ctgaccgatt
     1861 acgccgggaa cgatcgaagc atcctcggcc agatcgacct gatccaggga acgggcaaaa
     1921 accggtcctg atgaagcgcc atacctggaa ccaagcgaac cggccgcctc cttcaatgac
     1981 tccggttcaa cgccgagaat cgcggccagt gccgaataga gttttccgtc caagccgctg
     2041 gccggcagcg aaccccattc gagccggtgg tagtactcgt cgatcttttc ctgctcttgt
     2101 ggggtggcct caaaggtttt tgccaaagcg gcaaccaccg cagcctgggt cttgcctgcc
     2161 ttctgtctca gcagggcgag atgttcggaa agaccgcctg ccggagccga aacctcgtcg
     2221 aggaccttct cgaagacagc gttgaatcgg gggtcacccg ggtccgggct tgactcgggc
     2281 ccggttgcca cgaaggactc gatcagagtt tcgagggact tccgttcgct cccttcgaaa
     2341 cgctcgagga aaggcgacgg atcgccgtgg cccttgcgga actcggccac gtactcgtcg
     2401 aatgcgtgga tgaccgggtc cggggtttcc tcgaaatcgt tc
//
LOCUS       DAOQFI010000263         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_23786_length_2438_cov_2.753252, whole genome shotgun sequence.
ACCESSION   DAOQFI010000263 DAOQFI010000000
VERSION     DAOQFI010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23786_length_2438_cov_2.753252"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1488
                     /locus_tag="PLE93_05435"
     CDS             133..1488
                     /locus_tag="PLE93_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH86524.1"
                     /translation="MRGVAVRGTEEHDAGIRPDEAARKRVAVELIRNHDRPLRWTARR
                     YSICPEDAEDAYQRGIEILLSKAPTTDTRHLLPWTRKVIKHEALAIRRTRERTLARPG
                     QSDIDEHLEDDWVQLIPDISDGPDELTVRRERVARSREALTQLKPQELKALTQLAEGF
                     SYDEIGSMNSWTRTKVNRCLAEGRARFRSTFRESEAGERCPEFEPMISASCDGELTSS
                     QVAELETHLAVCGHCRSTLRAYRAAPRAAAALAPLLPAGRGLWERTQELAVDLQTRLS
                     GVGRGSDSGVQTVLASGGTRGAGMAAIAKIAAICAGTAGTAAVCAATGVLPAANLLPH
                     KDAKPVVERHASVARPEPERRKLDFVEPAPEPSTAPQSQAGGSPDQSATAAEPVSTTP
                     EPVSEAPVITESAPAPTPTETEFTPEAAGTPAPAAAPPPQPASSSSSVPVGNGGGEFS
                     P"
     gene            1485..>2438
                     /locus_tag="PLE93_05440"
     CDS             1485..>2438
                     /locus_tag="PLE93_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86525.1"
                     /translation="MNGFQKLAVVILGVLALGLVGASGANAGTYVMRQCDGSSFLDFQ
                     GTYSAINASPHFDAVSGCVTSGSGKLGIYQDRSGTSLAYGEGGHFVWAAPTGTEVIGA
                     RIAARLNDKNNIQAQLNGYNVGVHTDIDGGIPHDGSDQVSYWSDPAHPQWMVVIELLC
                     QSVNGCANQVGGTKAFLEVTDTEFTVRDTVAPTAAASGTLWDWGGDSGYHRGTASIGI
                     DATDQGSGVAATWVEINGLRVDLPAPTCPGRTERRNLLTDSPVRVATPPRPRYRPGES
                     GRPFPGWAGQAQGSTARDPGAGARGRGGSMSEVVATTRTILV"
BASE COUNT          452 a          771 c          823 g          392 t
ORIGIN      
        1 gctgcaaatg gacgcttttc gatggtttga aaatgtaggg gccaggttcc gtgggtgtga
       61 tttctgccgc tggcaatctt cgggtctccc ggataggaag agtgccaagc cgtctaagga
      121 gtgggggacc ccatgagggg agttgcggtt cgaggcacgg aagagcacga cgccgggatc
      181 aggccggatg aggccgcgcg caagcgggtt gcggtcgagt tgatcagaaa tcacgatcgc
      241 ccgctcaggt ggacggcacg ccgctactcg atctgtcccg aggacgccga agacgcctat
      301 cagcgaggca tcgagatcct tctctcgaag gcgccgacaa ccgacacgcg gcatctcctg
      361 ccctggacga ggaaggtcat caagcacgag gctttggcga ttcggcggac ccgcgagagg
      421 acgctcgccc gcccgggcca gtccgacatt gacgagcatc ttgaagacga ctgggtgcag
      481 ctcatcccgg atatcagcga cggcccggat gaactcaccg ttcgccgcga gagggtggcc
      541 cgcagccggg aagccctgac tcagctgaag ccccaggaac tgaaggccct gactcaactc
      601 gcagagggat tctcatacga cgaaatcggg tcgatgaact cgtggacccg caccaaggtg
      661 aaccggtgcc tggccgaagg gcgggctcgt ttccgaagta ccttccggga aagcgaagcg
      721 ggagagaggt gccccgaatt cgagccaatg atctcggcca gctgcgacgg ggagttgacc
      781 tcatcccagg ttgccgagct ggaaacccac ctcgccgtct gcggccactg tcggtcaacc
      841 cttcgcgcct atcgcgctgc ccccagggcg gccgcagcgc tggctcccct gttgccggcc
      901 ggccgggggc tctgggagcg aacccaggaa ctcgcggtgg acctgcagac tcgcctctcg
      961 ggagtcggcc ggggttcgga ctccggagtt cagacggtcc tggcctcggg aggtactcgt
     1021 ggtgccggga tggcggcaat cgccaagatc gcggcgatct gcgccggcac agcgggtacc
     1081 gcggcggttt gtgccgccac cggggtgctt ccggccgcga atctgctgcc ccacaaggac
     1141 gcaaaaccgg tcgtcgagcg gcacgcatcg gtagcccggc cagagcccga acggcggaag
     1201 ctggatttcg tggagcccgc gccggaacca tcgacggccc cgcaatcaca ggccggtgga
     1261 tctccggatc agtcggctac cgccgccgaa ccggtgtcga ccacccccga gccggtcagt
     1321 gaagcgccgg tgatcaccga gtcggcaccg gcaccgactc cgaccgaaac ggaattcaca
     1381 cccgaggcgg ccggaacgcc ggctcccgct gcagcacctc cccctcaacc agcttcaagt
     1441 tcaagttcag tgcctgtcgg aaacggagga ggggagtttt caccatgaac ggattccaga
     1501 aactggctgt cgtaatcctc ggggtactgg ccctgggcct ggtcggggcg agcggagcca
     1561 atgccgggac gtatgtgatg aggcaatgtg acggttccag ttttctcgat tttcaaggca
     1621 cctattccgc gatcaatgcg agcccgcatt tcgatgctgt gagcggatgc gtcacgtcgg
     1681 gttcgggaaa gctgggcatc taccaggacc gcagcggtac cagtctcgcc tacggtgaag
     1741 gcggtcattt cgtctgggcg gctcctaccg gaaccgaagt gatcggagcc cgcattgcgg
     1801 cccggctcaa tgacaagaac aacatccagg cccagctgaa tggctacaac gttggagtcc
     1861 ataccgacat cgatggtggc attcctcacg acggttcaga tcaggtcagc tactggtctg
     1921 acccagccca tccccagtgg atggtggtga tcgaactgct ctgccaatcg gtgaacgggt
     1981 gcgcgaacca ggtgggtgga accaaggcct tcctcgaggt aaccgacacc gagttcacgg
     2041 tcagggacac ggtcgcgccg acagcggcgg cctcgggaac cctctgggac tggggtggcg
     2101 attccggcta tcaccggggt actgcctcga tcgggatcga cgccaccgac cagggttcgg
     2161 gagtggctgc cacctgggtc gaaatcaacg gtctgagggt cgacctcccg gcgccgacct
     2221 gcccgggccg caccgagcgg aggaatctcc tcacggactc gccggtccgg gtggcaactc
     2281 ccccccgccc acggtatcgt ccgggggagt ccgggaggcc gttcccgggt tgggcggggc
     2341 aggcgcaggg ctcgaccgcg cgcgacccgg gggctggcgc ccggggccga ggagggtcga
     2401 tgagcgaagt ggtcgccacc acccggacca tcctggtc
//
LOCUS       DAOQFI010000264         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24141_length_2419_cov_3.069797, whole genome shotgun sequence.
ACCESSION   DAOQFI010000264 DAOQFI010000000
VERSION     DAOQFI010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24141_length_2419_cov_3.069797"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLE93_05445"
     CDS             complement(<1..213)
                     /locus_tag="PLE93_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86526.1"
                     /translation="MALGAGAGSASAMTVTSTGESGCSLRVAIQAVAANDSGTACGAV
                     VSGGPTPINLPANTITPFDGQIVVPAG"
     gene            complement(353..955)
                     /locus_tag="PLE93_05450"
     CDS             complement(353..955)
                     /locus_tag="PLE93_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH86527.1"
                     /translation="MEAAAEKQPRGPGRPSTGARERVLDAAVETLLEEGYAGLTYAKV
                     AVRAGENKSLISYYFGSKQGLVAAVADIIGERITVAVVSELNNVETVPDIAAGMIAGV
                     WKVMDEDPRLARLYFDLSAVSVVDDDVRDAIHLVKDRWREVIAGFMLDAGVRADRVAP
                     LGTFLMSGVQGLAIERLEGDQSDRLEAARKLFVRAAGAIA"
     gene            complement(971..1951)
                     /locus_tag="PLE93_05455"
     CDS             complement(971..1951)
                     /locus_tag="PLE93_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNH86528.1"
                     /translation="MSENGSSGNGNGEVEFKTEDEALQVLLDGDIDKVMSGLDRIIEA
                     APSYEKLFMRWQRQHWSTEDFDFSVDAEQWNDPDFMTEEERRFMLFGFSQFFLGEERV
                     TVELLPFAIAAPSHEAKAFLTTQISDEAKHMVFFDRFYREVLGMKAADIGAMLETQRP
                     NVNKDWEKLFDGILHDCAERLRLDPTDFPALVRGVTVYMIVIEGTLALTGARFIIKSL
                     KERGWLPGFVSGFTAVNRDESRHVGFGVKFLADAIKADQANAIVVQDTLKECLPITPL
                     VFAPSWVEDPYSFETPFYHSTEVFNYAMKALSKKLAAMGLDPAMLAGDPA"
     gene            2054..>2419
                     /locus_tag="PLE93_05460"
     CDS             2054..>2419
                     /locus_tag="PLE93_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86529.1"
                     /translation="MKRLAFLILLLPLAALAACGGKTSPEDDVTEVANQILEAKSPEQ
                     VKVVCQELITPGMLRTVYGGELARCEDQPLNDDSGIEDPGEVSVASVSVEGEKADAAI
                     VTTGGSTDGNDGTWYFRKTG"
BASE COUNT          460 a          785 c          762 g          412 t
ORIGIN      
        1 tccggctggc accacgatct gcccgtcgaa cggcgtgatc gtgttggccg gaaggttgat
       61 cggggtcgga ccgccgctga ccaccgcccc gcaggcggtg ccgctgtcat tcgcggccac
      121 cgcctgtatc gctacgcgca gcgagcagcc gctctcaccg gtcgaggtga cggtcatcgc
      181 cgaggcagat ccggcgccgg cccccagagc cacgccaatc gaaatgacgg aaaccagaag
      241 acgaaacgaa aacctgctca ctatgaggta cctcttcccg atatggaccc tgcgaaaacc
      301 ccacccttga ctctctaagg ttcaccgggt tatgtcaagg gggaagatcc ggtcaggcga
      361 tcgcgccggc ggcccggacg aaaagcttgc gggccgcttc gaggcgatcg gactgatcgc
      421 cctcgagccg ctcgatcgcc aggccctgga cacctgacat caggaaggtc ccgagcggcg
      481 cgacccggtc cgcccgaacg ccggcgtcca gcatgaagcc ggcgatcacc tcgcgccagc
      541 ggtccttgac caggtggatc gcatcacgaa cgtcatcgtc gaccaccgag accgcgctga
      601 gatcgaagta aagccgggcc aggcgggggt cctcgtccat gaccttccag actccggcga
      661 tcatgccggc agcgatatcg gggacggtct cgacgttgtt caactcggag acgaccgcga
      721 cggtgatccg ttcaccgatg atgtcggcga ccgcggcgac caggccctgc ttcgacccga
      781 agtaatagga gatcagggac ttgttctcgc cggcccggac cgcgaccttg gcgtaggtca
      841 gcccggcgta gccctcttca agcagagtct cgaccgccgc gtcgagcacg cgctcccggg
      901 ccccggtcga aggccgaccg gggccgcggg gctgcttttc ggctgctgcc tccaatcgcg
      961 agcctttggc tcaggcaggg tcgccggcca gcatggcggg atcgagaccc atcgccgcca
     1021 gcttcttcga caaggccttc atcgcatagt tgaagacctc ggtcgagtgg tagaagggcg
     1081 tttcgaagct gtacggatcc tcgacccagg aaggggcgaa gaccagcggc gtgatcggca
     1141 ggcattcctt cagggtgtcc tggaccacga tcgcgttggc ctgatcggcc ttgatcgcgt
     1201 cggcgaggaa cttgaccccg aagccgacgt ggcgcgattc gtcccggttt accgcggtga
     1261 acccggagac gaatccgggc agccagccgc gctctttcag cgacttgatg atgaaacggg
     1321 caccggtcag ggcgagggtg ccctcgatca cgatcatgta gacggtgacg ccacggacta
     1381 gcgccgggaa gtcggtcggg tcaagccgga ggcgctcggc gcagtcgtgc aggatcccgt
     1441 cgaagagctt ctcccagtcc ttgttcacgt tcggccgctg ggtttccagc atggcgccga
     1501 tgtcggccgc cttcatgccg agcacctcgc ggtagaagcg gtcgaagaag accatgtgct
     1561 tggcctcgtc cgagatctgg gtggtgagga aagccttcgc ctcatgtgac ggggcagcga
     1621 tcgcgaaggg cagaagctcg accgtcaccc gctcctcgcc gaggaagaac tgggagaagc
     1681 cgaagagcat gaagcgacgt tcctcctcgg tcatgaagtc cgggtcattc cactgctcgg
     1741 cgtcaaccga gaagtcgaag tcttcggtcg accagtgctg gcgctgccag cgcatgaaca
     1801 gcttctcgta ggaaggagcc gcctcgatga tccggtcgag accggacatc accttgtcga
     1861 tgtcaccgtc gagcagaacc tgaagcgctt cgtcttcggt cttgaactcg acttcgccgt
     1921 tgccgttacc tgaactgccg ttttcgctca tctctctcct gaagattttt gtggcctggg
     1981 cgaataataa acgaacgacc aacaaataat caagtctgtt tgccgaagcc ggccggcgag
     2041 gtacctaccg accatgaaac gtctcgcctt cctcatcctg ctccttcctc tcgccgctct
     2101 cgccgcctgt ggcggcaaga cctcccccga ggacgatgtg accgaggtcg ccaaccagat
     2161 cctcgaggcc aaatcccccg agcaggtcaa ggtggtctgc caggagctga tcacccccgg
     2221 gatgctcagg accgtctacg gcggcgagct cgccaggtgt gaggaccagc cgctgaacga
     2281 cgacagcgga atcgaggacc cgggcgaggt cagcgtcgcc tcggtctcgg tcgaaggcga
     2341 aaaggccgac gcggcgatcg tcaccaccgg cggttcaacc gacggcaatg acggcacctg
     2401 gtacttcagg aagaccggc
//
LOCUS       DAOQFI010000265         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24220_length_2414_cov_3.845697, whole genome shotgun sequence.
ACCESSION   DAOQFI010000265 DAOQFI010000000
VERSION     DAOQFI010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24220_length_2414_cov_3.845697"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLE93_05465"
     CDS             complement(<1..542)
                     /locus_tag="PLE93_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNH86530.1"
                     /translation="MATFTRVLVGHDGGKHAEDALSLAKLIAERTGAELVLASVIPNP
                     VGGSFGPALPAEAFTELAGKARASLEAAAAGVGAGVEVEQSSSAAHGLQAIAERISAD
                     LIVVGSSPSAETGHTRAGRKARQLLSGADCAIAVAPDGFHDRAGLDRIGVGIDGSPES
                     RLATETAVGLAPDGSELQLLA"
     gene            complement(613..1638)
                     /locus_tag="PLE93_05470"
     CDS             complement(613..1638)
                     /locus_tag="PLE93_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNH86531.1"
                     /translation="MTEHRDDSEMSRFDREGVRHFTARQSVLAVAIVAAILLVLSGGS
                     ILSAADEEKPGVEKDLLHAVGKPGSTLADNPLGRASAKLTSNLSPDEKLDPSQSFAQT
                     GTGAVTAGQIPPVTPSYFDPVELDDKAPPRQPLESLLVTGDSLSQPLDAELARRLTPE
                     GIEVTRDAHVGTGISNASLADWGQLSAAQVAEHHPDAVVVFIGANEGYPMPGPDGKDV
                     ACCSPEWAAIYANRARQVITNYRQGEKAKVYWLTVPLPRDPARQRIASVVNQAVAVAA
                     QPWRSQVRIVDTDEYFAPEGTYSDSIPVDGKDQIVRESDGIHLNDLGSSVLADHLLDR
                     IDLDFTR"
     gene            complement(1650..>2414)
                     /locus_tag="PLE93_05475"
     CDS             complement(1650..>2414)
                     /locus_tag="PLE93_05475"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNH86532.1"
                     /translation="ARVLVDPVFAVPQAYAAPDALLAAYGYAVQIYCDFSGYTDIAIG
                     LALLMGFIFPQNFNRPYRATGFRDFWRRWHMTLSRFLRDYLYIPLGGSRGSRLFTYRN
                     LMITMILGGLWHGAAWTFVLWGAFQGAALVTEHALDGRVGRRTPVWLRWFLTFNLVTF
                     AWILFRSQNLGLFWDYLGRFFVSGPATLYTLPVLLAIIVVIGMQLLPERPMERLQVRL
                     EQLNPALLAVMLAATILVVGATVPSQGVPPFIYFQF"
BASE COUNT          420 a          773 c          863 g          358 t
ORIGIN      
        1 gccagcagct gaagttccga gccatccggc gcgagcccga cggccgtctc ggtcgccagc
       61 cttgactccg gcgaaccgtc gatgccgact ccgattcgat cgagaccggc ccggtcgtga
      121 aacccgtccg gggcgacagc aatcgcgcag tcggcgccgg aaaggagctg cctcgccttt
      181 cggccggccc gggtatggcc ggtttccgcg ctggggctgg aaccgaccac gatcaggtct
      241 gcgctgatcc gttcggcgat tgcctgaagc ccatgggctg ccgaggagga ctgctcgacc
      301 tcaacgccgg caccgacccc ggcagcggcc gcctcgaggc tggccctggc tttgccggcc
      361 agctcggtga aggcctcggc cgggagcgcc ggaccgaaac tgccaccgac cggattcgga
      421 atgaccgagg cgaggaccag ttcggcgccg gtcctttcgg cgatcagctt cgcgagggaa
      481 agggcatctt ctgcgtgctt gccgccgtcg tggccgacca gcacacgggt gaatgtggcc
      541 atggctgaat gattccgccc ccggcccgaa cttgcaaggg gggaatctac ggaaccggca
      601 agtgaccgcg gctcagcggg tgaagtcgag gtcgatccgg tcgagcaggt ggtcggcgag
      661 gacggatgat ccgaggtcgt tgaggtggat cccgtccgac tcccggacga tctggtcctt
      721 gccgtcaacc gggatcgaat cgctgtaggt cccctccgga gcgaaatact cgtcggtgtc
      781 cacgatgcgg acctgcgaac gccacggctg ggcggccacg gctaccgcct ggttgaccac
      841 cgaggcgatc cgctggcggg ccggatcccg cggcaacggc acggtcagcc agtagacctt
      901 cgccttctcg ccctgccggt agttggtgat cacctggcgg gcccggttcg cgtagatcgc
      961 ggcccactcc ggggagcagc aggcaacgtc cttgccgtcc ggtccgggca tcggatagcc
     1021 ctcgtttgcg ccgatgaaca cgaccacggc atccgggtgg tgctcggcga cctgggccgc
     1081 cgaaagctga ccccagtcgg ccagcgatgc attggaaatc ccggtcccga cgtgagcgtc
     1141 ccgggtcacc tcgattcctt ccggggtcag ccgcctggcc agttcggcgt cgagcggctg
     1201 cgagagagag tcaccggtaa ccagcaggct ctccagcggc tgccgcggcg gcgccttgtc
     1261 atcgagctcg accggatcaa agtaggaagg agtgaccgga gggatctgcc cggcggtcac
     1321 cgccccggtt ccggtctggg cgaacgactg ggaagggtcg agcttctcgt ccggagagag
     1381 gttcgaggtc agcttggcgc tggcccggcc gagcgggttg tcggccaggg tcgacccggg
     1441 cttgccgacc gcatggagca ggtccttctc gacacccggc ttttcctcgt cggcggcgct
     1501 gaggatcgag ccgccggaaa gcaccagcaa aatcgcggcg acgatcgcca ccgccagcac
     1561 cgactggcgg gccgtgaagt gccgtacgcc ctcgcggtcg aagcggctca tttcactgtc
     1621 gtctcgatgt tcagtcacgg cgatgacccc tagaactgga agtagatgaa cggcggcacg
     1681 ccctggctgg gaaccgtggc gccgacgaca aggatggtag ccgcgagcat gacggccagc
     1741 agggcgggat tgagctgctc gagccggacc tggagccgtt ccatcggccg ttccggcaga
     1801 agctgcatgc caatcaccac gatgatcgcg aggagcaccg gcagggtgta gagcgtcgcc
     1861 ggcccggaga cgaagaatcg cccgaggtag tcccagaaga gcccgaggtt ctgggagcgg
     1921 aagaggatcc aggcgaaggt gaccaggttg aaggtgagaa accagcgcag ccagaccggg
     1981 gtgcgtcgcc cgacccggcc gtcaagtgcg tgctcggtga ccagggcggc gccctggaag
     2041 gcgccccaga gcacgaaggt ccaggccgcg ccgtgccaga ggccgccgag gatcatcgtg
     2101 atcatcaggt tgcggtaggt gaacagccgg ctgccgcggc tgcccccgag cgggatgtac
     2161 aggtagtcgc gcaggaagcg ggagagggtc atgtgccagc gacgccagaa gtcccggaag
     2221 ccggtcgccc ggtagggccg attgaagttc tgcgggaaga tgaagcccat cagcagggcc
     2281 agcccgatcg cgatgtcggt gtagccggag aagtcgcagt agatctgcac cgcgtagccg
     2341 taagccgcga gcagggcgtc cggtgcggcg taggcctggg gaaccgcgaa gaccgggtcg
     2401 accaggaccc gggc
//
LOCUS       DAOQFI010000266         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24241_length_2413_cov_3.197625, whole genome shotgun sequence.
ACCESSION   DAOQFI010000266 DAOQFI010000000
VERSION     DAOQFI010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24241_length_2413_cov_3.197625"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2413)
                     /gene="dnaE"
                     /locus_tag="PLE93_05480"
     CDS             complement(<1..>2413)
                     /gene="dnaE"
                     /locus_tag="PLE93_05480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNH86533.1"
                     /translation="RGNSTSTLASPGQMAYRFPDYPEAVSETVRVAERLVFDLERDLG
                     YRYPQQGDPKTDRDLAALCQTRIDERYRGSRQEPEARARLENELKIIRGLGLSGFFLL
                     HHDLLELAREVAVEVRGTSSARGVLPPGRGRGSSVSSIVCYLTGLSHIDPVKADLFPG
                     RFLNEEVTTAPDIDLDFPRDIRGRLIPRIHQRYGRDRSALVAAFPTYRSRGAIRDFGK
                     VLGIPDTEIERAARAVDFHSGSDDFQRDLTQAIGRERAHSAAWKHLALLSRQARSLPR
                     HASQHSGGMVIATRPLVEICPVVPAAMEQRQIVQWDKDSCQDAGFLKIDLLGLGMLSA
                     VERTIDEIHRIRGETVDLSRIDLDDRAVFGMIKRAETTGVFQIESRAQMQMLPRMRPD
                     NIDDLTIQVALIRPGPIQGGAVHPYIERRRRLREDPDYEIPFAHDLLRPVLGETHGVI
                     IFQEQVIEVAMHVAGFSSSEAESLRRAMSRKRSRRSLEAHHQRFIEGAMKSGVSREIA
                     DGIFEQIIGFSGFGFPKAHSAAFGLLAYQSAWLKHHYGPEYLASLLNEQPMGFYPPDS
                     LVQEARRTGVEVLPPDVNHSRVECVTEWPGSGGESSPRAESGPEGALARDEAATARRQ
                     QPAVRIGLGYVKGINESEMEKLVAERDRGGSFGDLGDLASRLPLARSDLEQLAWAGAL
                     RSLPRGERVAGLWSVGLTANRLPRAGGRQLALPFSQEETPELPEPKDWARVQAEYGSI
                     GMTLEGHPMELARRQLAESVLSTTDAASRPDRSWVEVAGLQVARQRPETAHGVIFILI
                     EDEHGT"
BASE COUNT          383 a          805 c          823 g          402 t
ORIGIN      
        1 gtgccgtgtt cgtcctcgat caggatgaag atcactccgt gggcggtttc cggtcgctgg
       61 cgggcaacct ggagaccggc cacctcgacc cagctgcgat ccggccggga ggcggcgtcg
      121 gtcgtggaca gcacgctttc ggccagttga cgacgggcca gctccatcgg atgtccctcc
      181 agggtcatgc cgatcgagcc gtattcggcc tggacccgcg cccagtcctt cggttccggg
      241 agttccgggg tctcctcctg actgaacggc agcgcgagtt gccgcccgcc ggcccgcggc
      301 aaccggttcg ccgtgagtcc gacgctccac agtccggcca cccgctcacc acggggcagg
      361 gacctgagtg ccccggccca ggccagctgc tcgagatcgc ttcgcgcgag tggcaggcgg
      421 gaggcgaggt cgccgaggtc gccgaagctg ccaccgcggt cacgctcggc gaccagcttc
      481 tccatctcgc tctcgttgat ccccttcaca taaccgagac cgatcctcac ggcgggctgc
      541 tgccgccggg ctgttgcggc ttcgtcccgg gccagggcac cttccgggcc ggactctgcc
      601 cgcggagagc tttccccgcc cgaccccggc cattcggtga cgcattcgac ccggctgtgg
      661 ttcacgtcgg gcggcaaaac ctcgaccccg gtgcgccggg cttcctggac cagtgaatcc
      721 ggcgggtaga agcccatcgg ctgctcgttc agaagcgaag ccaggtactc cggtccgtag
      781 tggtgcttga gccaggccga ctggtaggcc agcaggccga aggcggcgga atgagccttg
      841 ggaaagccga agccggagaa gccgatgatc tgctcgaaga ttccgtcggc gatctcgcgg
      901 ctgacgccgc tcttcatcgc gccctcgatg aaacgctgat gatgggcctc cagcgagcgg
      961 cgtgagcgct tgcggctcat cgcccttcta agcgactcgg cctctgagga actgaagccg
     1021 gccacatgca tcgctacctc gatcacctgt tcctggaaga tgatcacccc gtgggtctcg
     1081 ccgagcaccg gcctgagcag atcatgggcg aacgggatct cgtaatccgg gtcttccctc
     1141 agccttcgcc gcctctctat ataagggtga acggcgccgc cctgaattgg cccgggacgg
     1201 atcagtgcca cctggatggt caggtcatcg atgttgtccg ggcgcatccg gggcagcatc
     1261 tgcatctggg cccggctttc aatctggaag acaccggtgg tctcggcccg cttgatcatt
     1321 ccgaagaccg cccggtcatc gaggtcgatc cggctcaggt ccacggtttc accccggatc
     1381 cggtggatct cgtcgatcgt ccgctcgacc gccgagagca tgcccagccc gagcaggtcg
     1441 atcttgagga aacccgcgtc ctggcaggaa tccttgtccc actggacgat ctggcgctgc
     1501 tccatcgccg ccggtacgac cgggcagatc tcgaccagcg gccgggtcgc gatcaccatc
     1561 ccgcccgaat gctgtgaagc gtgacggggc aggctgcggg cctggcggga aagcagggcg
     1621 agatgcttcc aggccgccga gtgggccctt tcgcggccaa tcgcctgggt caggtcacgc
     1681 tgaaaatcgt cggagccgct gtgaaagtcc accgcccgcg cggctcgttc gatttccgtg
     1741 tcggggatgc cgaggacttt gccgaagtcg cggatcgccc cgcgggagcg ataggtggga
     1801 aaggcagcga ccagggcgga gcggtcccgg ccgtaccttt gatggatgcg ggggatcagc
     1861 cgccccctga tgtcccgggg gaagtcgaga tcaatgtcag gggccgtggt cacctcttcg
     1921 ttgaggaaac ggccggggaa gaggtcggcc ttgaccgggt cgatgtggga gaggccggtc
     1981 aggtaacaga cgatcgagct gaccgaggaa ccgcgtcccc ggcccggcgg cagcacgccc
     2041 cgggcggagg aggtgccacg cacctcgacc gccacctcgc gggccagctc cagcaggtcg
     2101 tgatggagca ggaagaagcc ggagagcccg aggccgcgga tgatcttcag ctcgttctcg
     2161 agacgggccc gggcctccgg ctcctgccgg gaaccccggt agcgctcgtc gatccgggtc
     2221 tggcagagcg ccgcgagatc ccggtcggtc ttcgggtcgc cctgctgcgg gtagcggtaa
     2281 ccgaggtcgc gttcgagatc gaaaacgagt cgctccgcga cccggaccgt ctcgctgacc
     2341 gcctccgggt aatccggaaa gcggtacgcc atctgaccgg gcgaggcgag ggtggaggtg
     2401 gagttgcccc gcc
//
LOCUS       DAOQFI010000267         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24249_length_2413_cov_2.545377, whole genome shotgun sequence.
ACCESSION   DAOQFI010000267 DAOQFI010000000
VERSION     DAOQFI010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24249_length_2413_cov_2.545377"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLE93_05485"
     CDS             complement(<1..421)
                     /locus_tag="PLE93_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNH86534.1"
                     /translation="MRIVYGVNGEGMGHATRSELVISDLLRDHEVRVVASGAAYRYLS
                     GIFEDVSQVFGPSFAMDQGRIQRWASFTGTLSSARRQLPANVREWISTIDKWKPEVAV
                     SDFEPLTGNYARLSGTPLVCVDNIHMIDRCRHDREITA"
     gene            complement(447..1022)
                     /locus_tag="PLE93_05490"
     CDS             complement(447..1022)
                     /locus_tag="PLE93_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH86535.1"
                     /translation="MTSAEKTEKTLRADARRNREKIFAAARELFARDAEGVQMEEIAS
                     HAGVGVGTIYRHFPTKQALLTEMVRTRFSRFTEIALEAERIDDPREAVETLIRQSCVE
                     VEGDAGFQLAMMSPDELEWEGIEQEKAAFAEPATRIIDRAVESGAVRSDFSFADFPML
                     MCGITSTMYFAPAGSDWQRHLDLILAGLRPG"
     gene            1173..>2413
                     /locus_tag="PLE93_05495"
     CDS             1173..>2413
                     /locus_tag="PLE93_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86536.1"
                     /translation="MVLALTQFIVVIDASITNVALPSIGKALDIDEKSLSWVVNSYTL
                     VFGGFLLLGGRLADFLGRRRMFMIGMTLFGAASLAGGFAQTEAWLIIARAVQGLGAAI
                     ASPAALSIVTTTFAEGSERNRALAIWGAVAGAGGAAGVLLGGVLTEWAGWEWVLFVNV
                     PIAAFVVWQAPQRLVESREEEESERTLDIPGAVTITAGLALLVYGLVDAVNAGWGSST
                     TMLRIAGALVLIAAFVVIELRSRKPLVPFSIFRNRTLRGANVVGLLTGMSLFSMFFLI
                     TLYLQLVLGLSALDAGLAYLPLAVSIILAAGMAGQLVTRLGFKPVVITGMVTVGIALA
                     WFSQISPDGTYLADVLVPSILAGIGLGLTFVPVTIAAMSGTKREEAGLASGLINTSQQ
                     VGGALGLAILSSIANSTTQDS"
BASE COUNT          366 a          811 c          779 g          457 t
ORIGIN      
        1 ccgccgttat ctcgcggtcg tggcggcaac ggtcgatcat gtgaatgttg tcgacgcaga
       61 ccagcggggt gccggaaagc cgggcgtagt tgccggtcag cggctcgaag tcactgaccg
      121 cgacttcggg cttccacttg tcgatggtcg agatccattc cctcacgttg gccggcagct
      181 gcctgcgggc ggaagagaga gtgccggtga aggaagccca gcgctggatc cttccctggt
      241 ccatcgcgaa cgaggggccg aagacctggc tcacatcctc gaagatcccg ctcaggtacc
      301 gatacgccgc tccggaggca acgacgcgga cctcatggtc ccggagcagg tcggagatga
      361 cgagctcgga gcgggtcgcg tgacccatgc cctcgccgtt taccccgtaa acgatcctca
      421 ttccgccagc ctacccggcg gcgcgctcag cccgggcgaa gtccggccag gatcaggtcc
      481 aggtggcgct gccagtcgga cccggccggg gcgaagtaca tggtcgaggt gatgccgcac
      541 atcagcatcg ggaaatcggc gaacgagaag tcgctgcgga cggcgccgct ttcgacggcc
      601 cggtcgatga tccgggtggc cggctcggcg aaggccgcct tctcctgctc gatcccttcc
      661 cactcgagct cgtcggggga catcatcgcg agctggaagc cggcgtcgcc ttcgacttcc
      721 acgcaggact ggcggatcag ggtctcgacc gcctcgcggg ggtcatcgat ccgctcggcc
      781 tcgagggcga tctcggtgaa gcgcgagaac ctggtccgga ccatctcggt caggagcgcc
      841 tgcttggtcg ggaagtgccg gtagatcgtg ccgaccccga ccccggcgtg gctggcgatc
      901 tcctccatct gcaccccctc ggcgtcgcgg gcaaacagct cgcgggcggc ggcgaagatc
      961 ttctcgcggt tgcgacgggc gtcagccctc agcgttttct ctgttttctc ggcggaagtc
     1021 atcgggttga caaacggaat ctggtttccg tatagtaccg caataagtgg aacaggaatt
     1081 ccgcttccga tttcaaaccg accagcgaat cccaggagca cctcttgtcc ggacctgacc
     1141 tcagcagccc tcgcgcgaag aaccttgcgc tgatggtgct tgccctcacc cagttcatcg
     1201 tggtgatcga tgcctcgatc accaatgtcg ccctgccctc gatcggcaag gccctcgata
     1261 tcgacgaaaa gagcctgtcc tgggtggtca acagctacac cctcgtcttc ggcggtttcc
     1321 tgctgctcgg cggtcgcctc gccgacttcc tcgggcgccg gcggatgttc atgatcggca
     1381 tgaccctgtt cggcgctgct tcactggccg gtggttttgc ccagaccgag gcctggctga
     1441 tcatcgcccg ggcggtccag ggtcttggcg ccgcaatcgc ttcacccgca gcactctcga
     1501 tcgtcaccac caccttcgcc gaaggctctg aacgaaaccg ggccctggcg atctggggcg
     1561 cggtggccgg tgccggcggc gcagccgggg ttctgctcgg cggcgtgctg accgaatggg
     1621 ccggctggga gtgggtgctc ttcgtcaacg tcccgatcgc ggcattcgtc gtctggcagg
     1681 ccccgcagcg tctggtcgag tcccgggagg aagaggaatc ggagcgcacc ctggacatcc
     1741 cgggcgcggt cacgatcact gccggcctgg ccttgctggt ctacggactg gtcgatgccg
     1801 tgaatgcggg ctggggatcg agcaccacaa tgcttcgaat cgccggcgcc ctggtcctga
     1861 tcgcggcctt cgtggtgatc gaactgcggt ccaggaagcc gctggttccc ttctcgatct
     1921 tccgcaaccg cacgctgcgt ggcgcgaacg tggtcggact tttgaccggc atgtcgctct
     1981 tctcgatgtt cttcctgatc acgctctacc tgcagctggt gctcggcctc tcggcgcttg
     2041 acgccggcct ggcctacctg ccgctcgcgg tatcgatcat cctcgccgcg ggcatggccg
     2101 gccagctggt caccaggctg ggcttcaagc cggtcgtgat caccggcatg gtcacggtgg
     2161 gtatcgccct cgcctggttc agccagatca gccccgacgg cacctacctg gccgacgtgc
     2221 tggtgccctc gatcctggcc ggcatcgggc tgggcctgac cttcgtgccg gtgaccatcg
     2281 ccgcgatgtc aggcaccaag cgcgaggaag ccggcttggc ttcgggcctg atcaacacct
     2341 cccagcaggt cggcggcgcg ctcggcctgg caatcctttc ctcgatcgcc aactcgacga
     2401 cccaggactc gtt
//
LOCUS       DAOQFI010000268         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24305_length_2410_cov_2.695117, whole genome shotgun sequence.
ACCESSION   DAOQFI010000268 DAOQFI010000000
VERSION     DAOQFI010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24305_length_2410_cov_2.695117"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLE93_05500"
     CDS             <1..253
                     /locus_tag="PLE93_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86537.1"
                     /translation="TRPERYEWEHELEGTPYESHLARQAIEARIAGDGEGSQVSLTST
                     NVLRGTAKIAGFSMKKGQRELLDEALESLAETLEPGGGS"
     gene            250..1908
                     /locus_tag="PLE93_05505"
     CDS             250..1908
                     /locus_tag="PLE93_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNH86538.1"
                     /translation="MTEPRRDSRWWGWGDPDVPTELGEAAERMLGERGISTGADASPP
                     EIDRVEIPEAVPVDERVLSAAGAENVFTGNEDRIRHANGQNYLDLLELREGRLQHAPD
                     AVIVADSGPRIAAILEACASAGVAVVPFGGGSSVVGGVTPLKGDHESVISLDLAGLRG
                     IEVDDRSLTAKLGAGLRGPEAEALLAERGFTLGHYPQSFEYATIGGFAATRSAGQSSS
                     GYGRFDSLVSSIRLITPEGELSTLSTPHTSIGPALREVVVGSEGILGVIPDVDVRIRP
                     LPAARRYEAWIVEDFEAGCEIVRGLAQADRLPTIIRVSDDSETEVNLAMALPSGAAGS
                     LFRRYLKLRGREGGALVITGYEGSPAAVHRSRSDVAREIRRGGGVYLGQAAGKGWEKG
                     RFHGPYLREALLERGVLVETLETAQQWSGHRNLYETVRAALKSELAANGMEGIVMCHL
                     SHAYRDGASLYFTVISTQGPDGGAANWPKVKRAACAAIQSAGGTLSHHHATGRDHAPW
                     IEGEIGTSGIEALAALKERFDPSGIMNPGKLLDASRAGSDPPSR"
     gene            complement(1911..>2410)
                     /locus_tag="PLE93_05510"
     CDS             complement(1911..>2410)
                     /locus_tag="PLE93_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86539.1"
                     /translation="ERRGGQVQQVAVAVLQPVGNEQGSGQVQFGFSGTSLAANVDISG
                     LQPNEKGQGYVIWLYGSTGAFPIYAQKVGKSGAITGQIGPLNNAVICLIASDFFPTLR
                     VSRADNDQFDQALKDASPTKNSKVKLPEYTGQTVLEGAISMPQKAKDTIVPVCNGTAN
                     QSQSG"
BASE COUNT          392 a          785 c          836 g          397 t
ORIGIN      
        1 gacccggccc gagcgctacg agtgggaaca cgaactcgaa ggaacgccgt acgagtccca
       61 ccttgcccgc caggcgatcg aagcccggat cgccggcgac ggcgagggtt cgcaggtgag
      121 cctcacctcg accaacgttc tgcggggaac cgcgaagatc gccggcttct cgatgaagaa
      181 gggccagcgg gagctgctcg atgaagcgct cgaatccctc gccgagaccc tcgaaccggg
      241 cggcgggtct tgaccgagcc acggcgcgac agccgctggt ggggctgggg cgacccggat
      301 gtgccgaccg aacttggcga agccgcggag cggatgcttg gcgaacgtgg catcagcacc
      361 ggcgccgacg cctcgccacc ggagatcgac cgggttgaga ttccggaagc cgtgccggtc
      421 gatgaacgtg tcctgagcgc tgccggtgcc gaaaacgtgt tcaccgggaa cgaggaccgg
      481 atccgccacg ccaacggcca gaactatctc gacctgctcg agttgcgcga gggccggctt
      541 cagcacgctc ccgacgcggt gatcgtcgcc gactccggcc cgcggatcgc agcgattctc
      601 gaggcttgtg cctcggccgg agtcgcggtg gtgcccttcg gcggcggcag cagcgtggtc
      661 ggtggcgtta ctccgctgaa gggcgatcac gagtcggtga tcagtcttga cctcgccggg
      721 ctgaggggaa tcgaagttga cgatcgctcg ctgaccgcga agctgggggc gggcctgcgc
      781 ggccccgagg ccgaagccct gctggccgaa cgcggcttca ccctcgggca ctatccccaa
      841 tcctttgagt acgcgacgat cggcggtttc gccgccactc gctcggccgg ccagtcatcg
      901 agcggctacg gtcgcttcga ttcactggtc agttcgatcc gcctgatcac gccggagggg
      961 gaactctcga ccctttcgac tccccacacc tcgatcgggc cggccctgcg cgaggtcgta
     1021 gtcggttcgg aaggaatcct cggggtgatt cccgacgtgg acgtaaggat ccggcctttg
     1081 cccgccgccc ggcgctacga ggcctggatc gtcgaagact tcgaagccgg ttgcgagatc
     1141 gttcgcgggc tggcccaggc ggaccggctg ccaaccatca tcagggtttc cgacgattcc
     1201 gaaaccgagg tcaacctggc gatggccctg ccttccggcg cggccggttc acttttccgg
     1261 cgctacctga aactgcgggg ccgggagggc ggggcgctgg ttatcaccgg ctacgagggc
     1321 agcccggccg cagttcatcg cagccgctcc gacgtggccc gcgagatccg caggggtggc
     1381 ggggtctatc tgggccaggc ggccggcaag ggctgggaga agggccgctt ccacgggccg
     1441 tacctgcgcg aggccctgct ggagcggggc gtcctggtcg agacgctcga gaccgcccag
     1501 caatggagtg gccacaggaa cctctacgag acggtccgcg cggccctgaa gagcgaactg
     1561 gctgcgaacg ggatggaagg catcgtcatg tgccatctct cgcatgccta ccgcgacggc
     1621 gcttcgctct acttcacggt gatctccacc caggggccgg acggcggggc ggccaactgg
     1681 ccgaaggtga agcgtgcggc ctgcgcggct attcagtcgg ccggggggac gctctcacat
     1741 caccacgcca ccggccgtga ccatgcgccc tggatcgagg gggagatcgg aaccagcgga
     1801 atcgaggccc tggccgccct caaggaacgc ttcgacccgt ccggcatcat gaaccccggc
     1861 aagttgcttg atgcgagccg ggcggggtcg gacccgccct cccggtagcg ctatcccgac
     1921 tggctctggt ttgcggtgcc gttgcagacc gggacgatcg tgtccttggc cttctggggc
     1981 atggagatcg cgccttcaag cacggtctgg ccggtgtact cgggaagctt gaccttgctg
     2041 tttttggtcg ggctggcgtc cttgagcgcc tgatcgaact gatcgttgtc ggctcgggac
     2101 acccgcaggg tggggaagaa atcggaggcg atcaggcaga tcaccgcgtt gttgagcggt
     2161 ccgatctggc cggtgatcgc cccggacttg ccgaccttct gggcataaat gggaaaggca
     2221 ccggtcgagc cgtaaagcca gatcacgtaa ccctggccct tttcattcgg ctgaagtccg
     2281 gagatgtcca cgttggccgc cagcgaagtt ccggagaagc cgaactgaac ctggcccgag
     2341 ccctgttcgt tgccgaccgg ctggagtacc gcgacggcga cctgctgtac ctgcccgcca
     2401 cgtcgctcgg
//
LOCUS       DAOQFI010000269         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24395_length_2405_cov_2.846383, whole genome shotgun sequence.
ACCESSION   DAOQFI010000269 DAOQFI010000000
VERSION     DAOQFI010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24395_length_2405_cov_2.846383"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLE93_05515"
     CDS             <1..550
                     /locus_tag="PLE93_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNH86540.1"
                     /translation="KAFGSDGGLWLAALSPAAIARIEELDGTSGRWQPPFLSLKTALD
                     NSRKDQTYNTPALATLYLLAEQLDWMLAGGGLEFCVGRTRASSSHLYGWAEASDFATP
                     FVTDPALRSMVVGTIDFDDSIDAAAVAATLRANGIVDVEPYRKLGRNQLRIAMFPAVD
                     PADVEALTACVDWVIGNAGVTA"
     gene            603..2195
                     /gene="serA"
                     /locus_tag="PLE93_05520"
     CDS             603..2195
                     /gene="serA"
                     /locus_tag="PLE93_05520"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNH86541.1"
                     /translation="MSERAKVLVKEKIADSGVEQLKENFDVEIGVDMDDAELAERIGE
                     FDAILIRSATKLTPELIEKADNMKVIGRAGTGVDNVDIPAATKRGIIVANAPESNSVA
                     AAEHTMALALALFRNVPQAHSALVDGRWDRAKYKGAELYGKTLGVIGFGRIGQLVAKF
                     AQGFEMEVLAFDKFVSHERFRDLGVEGVDSAEEIYRRADLITIHLPKTPETVDWIDAE
                     AIARFKPGMRIVNCARGELVNLDALIDGLESGQLAGAALDVFPEEPFTEHPIFERDDV
                     VVTPHLGASTAEAQDRAGTDTAEQVTAALTGGVVTNAVNIPAVSPAAMEAVAPYVPLC
                     ETLGKVAVGLTPGSIDRVEVEFRGGIAENDTRLLGIAVQAGLLSGHTEEAVNLVNAPQ
                     MAEERGIEVIEIKDTTADEFTDLVRVTIQSGADVVTVGGTTVGPKNAPYLVSIWGQGF
                     YLPFAEHMAVFRYADQPGMIGKVGSLFGEEGVNIGSAAVGAEADSDNAVMVVTSDAPV
                     SAQTIETIKAVDGFSAGYAIEL"
BASE COUNT          418 a          773 c          823 g          391 t
ORIGIN      
        1 gaaggcgttc ggttccgacg gcggtctctg gctggccgcg ctgagccctg ccgccattgc
       61 ccggatcgag gagctggacg gtacatccgg ccgctggcag ccgcccttcc tgtcgctgaa
      121 gacggcgctc gataactccc gcaaggatca gacttacaac acccccgcgc ttgccaccct
      181 ttacctgctg gccgagcagc tcgactggat gctcgcgggc gggggcctcg aattctgcgt
      241 cgggcggacc cgcgcttcgt cctcgcacct ttacgggtgg gccgaggcaa gtgatttcgc
      301 gactcccttc gtgaccgacc cggcgctgcg gtcaatggtc gtcggaacga tcgatttcga
      361 cgactcgatc gacgcggccg cggtcgcggc gacgctgcgg gccaacggca tcgttgacgt
      421 cgagccgtac cgcaagctcg gccgaaacca gttgcggatc gcgatgttcc cggcggtcga
      481 ccccgctgac gtcgaggcgc tcaccgcctg cgtcgactgg gtgatcggga acgccggggt
      541 caccgcgtga agagacttga cccacaccgg ttctcccgga ccggccaacc aggaggattg
      601 atttgagcga gcgagccaag gtactcgtca aggagaagat cgccgacagc ggcgtcgagc
      661 agctgaagga gaacttcgac gtcgagatcg gcgtcgacat ggatgacgcg gaactggccg
      721 aacggatcgg cgagttcgac gcgatcctga tcaggtcggc caccaagctc actcccgagt
      781 tgatcgagaa ggccgacaac atgaaggtga tcggccgggc cggcaccggg gtcgacaacg
      841 tcgacatccc ggccgcgacc aagcggggca tcatcgttgc caacgccccc gaatcgaatt
      901 cggtcgcggc ggccgagcac acgatggctc tcgccctggc cctgttccgc aacgtgccgc
      961 aggcccattc cgccctcgtt gacggccgct gggaccgggc aaagtacaag ggcgccgagc
     1021 tctacggcaa gaccctgggc gtgatcggct tcggccggat cggccagttg gtcgccaagt
     1081 tcgcgcaggg attcgagatg gaggttctcg ccttcgacaa gttcgtctcc cacgagcggt
     1141 ttcgcgatct cggcgtcgag ggggtcgact cggccgagga gatctaccgc cgtgcggacc
     1201 tgatcacgat ccacctgccg aagaccccgg agaccgtcga ctggattgac gccgaggcga
     1261 tcgccaggtt caagcccggc atgcggatcg tcaattgtgc ccgcggtgag ctggtcaatc
     1321 tcgatgcgct gatcgacggc ctcgaatcgg gtcagctggc cggggccgcc ctggacgtgt
     1381 tcccggagga gccgttcacc gagcatccga tcttcgagcg ggatgacgtg gtcgtgacgc
     1441 cgcatctcgg cgcttcgacc gccgaggccc aggaccgggc cggtaccgac acggccgagc
     1501 aggtgaccgc cgccctgacc ggcggggtgg tgaccaacgc ggtcaacatt ccggctgtga
     1561 gtccggccgc gatggaagcg gttgcgccct atgtgccgct ctgcgagacg ctcggaaagg
     1621 tcgcggtcgg cctgaccccg ggttcgatcg accgggtcga ggtcgaattc cgcggcggca
     1681 tcgccgagaa cgacacacgg ctgctcggga tcgcggttca ggccggtctt ctttccggcc
     1741 acaccgagga ggcagtgaac ctggtcaacg ctccgcagat ggccgaggaa cgcggcatcg
     1801 aggtgatcga gatcaaggac accacggccg acgagttcac ggacctggtc cgggtgacga
     1861 tccagtcggg agccgatgtc gtaaccgtcg gaggaaccac ggtgggcccg aagaacgcgc
     1921 cttatctcgt ttcgatctgg ggtcagggct tctacctgcc gttcgccgag cacatggccg
     1981 tcttccgcta cgccgaccag ccggggatga tcgggaaggt cggatcactg ttcggcgaag
     2041 aaggcgtcaa catcggttcg gccgcggtcg gtgcggaggc agacagcgac aacgcggtga
     2101 tggtcgtgac ctccgatgcc ccggtttcgg cgcaaacgat cgaaacgatc aaggcagtcg
     2161 acggtttctc ggccggatac gcgatcgagc tctgatcaac ccggcttccg cggataggct
     2221 ggagccatga gagctgtcgt cctgaccgga cacgggggac cggatgacct gaaggttcag
     2281 gagcgtccgg acccgccggc cggtccgggc gaggtgcggg tcgcggtcaa ggcggccgga
     2341 gtcaacttcg cggacgtgat ggcaacccgg ggtctttacc cggacgcgcc caagccgccc
     2401 tgcgt
//
LOCUS       DAOQFI010000270         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24426_length_2403_cov_4.224446, whole genome shotgun sequence.
ACCESSION   DAOQFI010000270 DAOQFI010000000
VERSION     DAOQFI010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24426_length_2403_cov_4.224446"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLE93_05525"
     CDS             complement(<1..890)
                     /locus_tag="PLE93_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNH86542.1"
                     /translation="MKLAVLGVGLIGGSIGLAARDRDLAEVTGWVRNPDRAEECLKVG
                     AVDHMAATVEEACADADVIVCCASVGHLIEMGRKALEAAPEGCVVTDVGSTKQELVEA
                     LGNDRRFIGGHPLAGAETAGVMNARADLYEGARWYLTPTSDSEGMLLDRLQKLVKGLG
                     AKPVAIDAVEHDRAMAVVSHLPHVFANQLAAQGRPGTERLGALAPSLRDGTRVAGSNP
                     AIWGEIFATNHEAVVAAIDESIAGLERAREVIGSADPGRIEQWQAAVGEDRAALSATE
                     TAGEATHELRVLVPNQPGVLA"
     gene            complement(901..1914)
                     /gene="aroF"
                     /locus_tag="PLE93_05530"
     CDS             complement(901..1914)
                     /gene="aroF"
                     /locus_tag="PLE93_05530"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNH86543.1"
                     /translation="MKEGATEEEIAHVVERVEEIGCSAHISEGELLTVIGAIGDRGKV
                     ADLGLEGAPGVDHVTPISKPYKLASRETKQGADSVLEIGGRKVGGTNFALMAGPCTVE
                     NRDQMLDSADAVADAGATMLRGGAYKPRTSPYSFQGLGREGLRLLSEARERTGLPIVT
                     ELLDLRDIDDVVEVADVIQIGARNMQNYALLSEAGKSGKAVLLKRGLSASIEELLMAS
                     EYILKEGNENVMLCERGIRTYETAYRFTLDLLAVPALKEQTHLPVIVDPSHAPGRRDW
                     VESMSLAAAASGADGIIIEVHNEPEKAICDAAQQVPTSEFQELTNKLETITSVIGKTI
                     SRA"
BASE COUNT          372 a          842 c          757 g          432 t
ORIGIN      
        1 gccagcacgc cgggctgatt cgggaccagc acccgcagct cgtgggtcgc ttcaccggcg
       61 gtctcggttg ccgaaagcgc cgcccggtcc tcgccgacgg cggcctgcca ctgctcgatc
      121 cgcccggggt cggccgagcc gatcacttcc ctggcgcgct cgagtccggc gatcgactcg
      181 tcgatcgcgg cgaccaccgc ctcgtgattg gtggcgaaga tctcgcccca gatcgcgggg
      241 ttcgagccgg cgacgcgggt gccgtcacgg aggctcggag cgagggcgcc gagccgctcg
      301 gtgcccggcc gaccctgggc ggcgagctga ttggcaaata catgtggcag gtggctgacc
      361 accgccatcg cccggtcgtg ctcgacggcg tcgatcgcga ccggtttcgc gccgaggccc
      421 ttgaccagct tctgcagccg gtcaagcagc atcccctcgg agtccgaggt cggggtcagg
      481 taccagcggg caccctcgta gagatcggca cgggcgttca tcaccccggc cgtctcggcg
      541 ccggccagcg ggtgaccccc gatgaatctc cggtcgtttc cgagcgcttc gaccagttcc
      601 tgcttggtcg aaccgacatc ggtcaccacg cagccttcgg gggcagcctc gagagccttg
      661 cggcccatct cgatcagatg cccgaccgag gcgcagcaga cgatcacgtc cgcgtcggcg
      721 caggcctcct cgaccgtggc cgccatgtga tcgaccgccc cgaccttgag gcattcctcc
      781 gcccggtccg ggttccgcac ccagccggtg acttcggcca gatcccggtc ccgtgccgcg
      841 agaccgatcg agcctccgat cagcccgact ccgaggacgg ccagtttcat cgaaccccct
      901 ctaggcccgc gagatcgtct tgccgatcac cgaggtgatc gtctccagct tgttcgtcag
      961 ctcctggaat tcggaggtcg gaacctgctg ggccgcatcg cagatcgcct tctcgggctc
     1021 gttgtgaacc tcgatgatga tcccgtccgc accggaagcg gcagcggcaa gggacatcga
     1081 ctcgacccag tcgcgacgac ccggtgcgtg gctcggatcg acgatcaccg gcaggtgcgt
     1141 ctgctccttc agggcaggca ctgccagcag gtcgagcgtg aagcggtagg cggtctcgta
     1201 ggtccggatg cccctttcgc agagcatcac gttctcgttg ccttccttga ggatgtactc
     1261 ggaggccatc agaagctctt cgatcgaggc cgaaagcccg cgcttcagca gcacggcctt
     1321 gcccgacttg cccgcttcgg aaagcagcgc gtagttctgc atgttgcggg cgccgatctg
     1381 gatcacgtcc gccacttcga ccacgtcgtc gatgtcgcgc aggtcgagca gctcggtcac
     1441 gattggcagg ccggtacgct cgcgcgcttc ggaaagcagg cgcagccctt cgcggccgag
     1501 gccctggaag ctgtacggag aggtccgggg cttgtaggcg ccgcctcgca gcatcgtcgc
     1561 gccggcatcg gccacggcgt cggccgagtc cagcatctgg tctcggtttt caaccgtgca
     1621 aggacccgcc atcaaggcga agttcgtgcc cccgactttt cggccgccga tctcgagaac
     1681 cgagtcggca ccctgcttgg tttctcggga cgccagcttg tagggcttcg agatcggagt
     1741 cacgtggtcg accccgggtg ctccttccag accgagatcg gcgaccttgc cgcgatcccc
     1801 gatcgccccg atgacggtca gcaattctcc ctcggaaatg tgcgccgaac agccgatctc
     1861 ctcgacccgt tcaacgacat gggcgatctc ttcttcggtc gccccctctt tcatcacgat
     1921 catcattgtt ttttgcttct acctttcctg tactgccaaa gaccttacct gcgttcgtcc
     1981 ggccgaaggc agcggacgac accgaatgac acggcgcccg gcggctgtga attcactggc
     2041 gggcagctac ccgcatcacc ggacggacgc ctagataaat cgccaatagg aataaaaggc
     2101 agcaccgggg gttccgctct tgcggagggc cgtgcccgaa ggggcgatgc ggctgtcggt
     2161 ctgctgcggc attgggccag tttgtatcag gtcgcccggc ttagcgccaa cgatcggccg
     2221 cgcggggcgc tagccgtgcg ccttgatcgc ttcgccgagc gcggccacga aacggtcgtt
     2281 ttcttccggc gttccgtagg aaacccgcag ccagccgggg ccgccgagca gtttgccggc
     2341 ccggaccacg atctccgcct cggccagcga accgaccacc gcatcttcga tctccgcacc
     2401 gtt
//
LOCUS       DAOQFI010000271         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24477_length_2400_cov_3.477186, whole genome shotgun sequence.
ACCESSION   DAOQFI010000271 DAOQFI010000000
VERSION     DAOQFI010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24477_length_2400_cov_3.477186"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLE93_05535"
     CDS             <1..456
                     /locus_tag="PLE93_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86544.1"
                     /translation="NGHHLADGVVGPWKLKLGATAVTSGESSTTSTVPVDLGGPTATV
                     KVPEGGMVAIQAEAMMRATGSNAARVYLYDPLSVPNPVMIHRSEANDFDLRISTPGVG
                     ASDGVTSRVRAGWIVMPVTAGTHSFQLRYSTSGGTAIFKDRVLRVAVIR"
     gene            complement(460..669)
                     /locus_tag="PLE93_05540"
     CDS             complement(460..669)
                     /locus_tag="PLE93_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86545.1"
                     /translation="MAREVFDQARPRSTKAQRFADAFKGCEEWTVPPRKPRSMSDSVF
                     NAEESPFDSRIYGAEIIDLEFREAA"
     gene            1326..1550
                     /gene="rpmE"
                     /locus_tag="PLE93_05545"
     CDS             1326..1550
                     /gene="rpmE"
                     /locus_tag="PLE93_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNH86546.1"
                     /translation="MKADIHPDYKLANVHCSCGNQFYTRSTKEDLHVELCSACHPFYT
                     GKQKLVDTGGRVERFQRRAAKRAKATAGQN"
     gene            1593..>2400
                     /locus_tag="PLE93_05550"
     CDS             1593..>2400
                     /locus_tag="PLE93_05550"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNH86547.1"
                     /translation="MQSNGASPTRSGAVSPAPLEAKRDAPVGGQAVLEGVMMRGVTTW
                     AVSVRKPDGEIETTTEKIEPWARRHRVLRWPIIRGIVALAESLKIGFRALGMSANAQL
                     DEGEEEEELGGMAWGMTIFVSLAFAILLFFVIPVGLTSLIKDWLDSSFLFWLVEGIVR
                     TAIFIGYIVIISRLEDLRRVFEYHGAEHKTISCYEAEDELIPSRAALYSRLHPRCGTS
                     FLLIVMVVAIFVFAPLGLPAWYWLVISRIVGVPLTAGVAYELIKWAGRNRD"
BASE COUNT          415 a          768 c          778 g          439 t
ORIGIN      
        1 aacggccatc atctggccga cggggtggtt ggcccctgga agctcaaact gggagcgacc
       61 gccgtcacct ccggagagtc gtcaaccact tcgacggtgc cggttgacct gggcggcccg
      121 accgccacgg tcaaggtgcc ggagggcggg atggtcgcga tccaggccga ggcgatgatg
      181 agggccaccg gcagcaacgc cgcgcgggtc tacctctacg acccgctctc ggtcccgaat
      241 ccggtgatga tccaccgctc ggaagccaac gatttcgatt tgaggatctc gacgccgggc
      301 gtcggagctt cggacggagt gaccagccgg gtcagggcgg gatggatcgt catgccggtg
      361 acggcaggaa cccacagctt ccagctgcgc tacagcacgt caggcggaac cgcgatcttc
      421 aaggatcgcg tcctgagggt ggctgtgatc agatagtgct caggctgctt cgcggaattc
      481 caggtcgatg atctcggctc cgtaaatgcg tgaatcgaac ggcgattctt cggcattgaa
      541 cacggaatcg gacatcgatc ggggcttccg cggcgggacg gtccactctt cgcagccctt
      601 gaaagcatcg gcaaaccgct gcgccttggt actacggggg cgagcctggt cgaatacctc
      661 tctggccata cggacaatcc tcctccggaa ggggttatgg aaagctagtt cgccccatcg
      721 gacggaaaac ctgccaattt tcttgcaatt tgcgaaattc ccgcggcgcc acgagtccgc
      781 gccgcacttt ccgctccgtt agccgcggcc ggtgccgcag gtttacgacc ccagaggtcg
      841 caaatctgcg gcacctcgaa gtcggcaggg ctggtgaagg gggagtgggt cggagtctcg
      901 ggcggattcg agacgggagt tatcggcaaa tagcgcaccc aactccgact tcgatccggg
      961 ccgcccattc gacatcggag ttgagtgcga tatccggcca aaagtggact ctcgcgaatc
     1021 gcctccggag ggccagggca gccccttggg gcggcatggg tccgattagc cgtggggtcg
     1081 ggtgggggtc gttgaggtgt ctcccgggtc cctgtagccg tggggtcggg tgggggtcgt
     1141 ccgggacact ccaggaccag cgcggctgaa tgccgcgcgt gcgtccgtcc cggctacccc
     1201 cacccgaccc cacggcgtaa ccaacggccc ccgcggcgta accaccggcc cccaggcgtg
     1261 actacttgtg cccccacggc gagcagagaa gtcggtggct tgacctcttc tctagaatgt
     1321 gtttcatgaa ggcagatatc catcccgatt acaagctcgc gaacgtccat tgttcgtgcg
     1381 gcaatcagtt ctacacgcgg tcgaccaagg aagacctgca tgtcgaactc tgctctgcct
     1441 gccatccctt ctacaccggc aagcagaagc tggttgacac cggcggacgg gtcgagcgct
     1501 tccagcgccg cgctgccaag cgcgccaagg ccaccgcagg ccagaactag gttccgcccg
     1561 ggccggctgc cggcccgcga gaccaacagc gaatgcagtc gaacggcgcc tcaccgaccc
     1621 ggtctggcgc cgtttcgcct gctcctctcg aagccaagcg cgacgccccg gtagggggtc
     1681 aggcggtgct cgaaggggtg atgatgcgtg gcgtcaccac ctgggcggtc tcagtccgca
     1741 agccggacgg cgagatcgag acgaccaccg agaagatcga accctgggcc cgccggcacc
     1801 gggtattgcg ctggccgatc atccgcggca tcgtcgccct ggccgaatcg ctgaagatcg
     1861 gatttcgggc cctgggcatg tcggccaacg cccagctcga cgagggagag gaggaagagg
     1921 aacttggcgg catggcctgg gggatgacga tcttcgtctc gcttgccttc gcgatcctgc
     1981 tcttcttcgt gattcccgtc gggttgacca gcctgatcaa ggactggctt gactcttcct
     2041 ttctcttctg gctggtcgaa gggatcgtcc ggaccgcgat cttcatcggt tacatcgtga
     2101 tcatctcccg gctggaggac ctgcggcggg tcttcgagta ccacggcgcc gagcacaaga
     2161 cgatctcctg ctacgaagcc gaggacgaac tgattccttc ccgggccgcc ctctactcgc
     2221 ggctccatcc gcgttgcggc accagcttcc tgctgatcgt catggtcgtc gcgatcttcg
     2281 tcttcgctcc gctgggcctg cccgcctggt actggctggt gatttcgcgg atcgtcgggg
     2341 tgccactgac cgccggcgtc gcctacgagc tgatcaagtg ggccgggcgc aaccgggata
//
LOCUS       DAOQFI010000272         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24639_length_2392_cov_3.121095, whole genome shotgun sequence.
ACCESSION   DAOQFI010000272 DAOQFI010000000
VERSION     DAOQFI010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24639_length_2392_cov_3.121095"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PLE93_05555"
     CDS             complement(<1..1340)
                     /locus_tag="PLE93_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86548.1"
                     /translation="MTGKFEAVEEEILVGAPTPAAGPVPKEMAEAEAAYRPEVLGKVL
                     AVVAAAVALASLDMFIVNIAFPAIEADFPGSSIGSLSWVLNGYVIVFAALLMPFGRLA
                     DRVGRKRIFTTGVLIFCLASGACAAAWSVETLVAFRVIQGIGAAMLMSTSLALLLHAF
                     PAERWPVVIGVWAAAGGAAGALGPPIGGLLVEISWRWVFLVNLPLSLAAWYFGTRLLV
                     ESKDPRETRWPDPAGMALMILGIGVLCWGLVEAPDHGWGSIRTVVAILAGLVMLGLTF
                     LRSRRPADRWIPTLDVDLFKARPFAMACLTALIFMVAFAAILLGGVLFLTTVWGFSVL
                     EAGIAFFPGPAMAGIFSVLGSRFGARFGTGPVAATGCGFLILGGTWWFTRMGDTSAYF
                     TENLPGQILVGIGVGLVLPNVAAAVASTLPPTSLATGTAVLSASRQVGAALGVA"
     gene            1440..1919
                     /locus_tag="PLE93_05560"
     CDS             1440..1919
                     /locus_tag="PLE93_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH86549.1"
                     /translation="MLDREYDNQVCSIARSLEVVGERWSLLIIRNVGLGLNRFEPLRE
                     NLGITRSVLTTRLNTLIEYGILEKRQYSEKPPRFEYHLTQKGRDLSPVLLQLMWWGDE
                     YYPEPGGPPTIAVHRTCGGNLDHHMVCEKCGESVEPDRVYMKPGPGRRRQDLPKIPA"
BASE COUNT          485 a          839 c          735 g          333 t
ORIGIN      
        1 atggccacac ccagcgcggc gccgacctga cgggaagcgc tcagaactgc cgtcccggtc
       61 gccagcgagg ttggcggcag ggttgaagcc acagctgctg cgacgttcgg caggacgagt
      121 ccgaccccga tgccgaccag gatctgtccg ggcaggttct cggtgaagta ggcggatgta
      181 tcccccatcc gggtgaacca ccacgttccc ccgaggatca ggaacccgca gccggttgcg
      241 gccaccggcc cggtgccgaa ccgggctccg aaccggctgc cgagaaccga gaagatcccg
      301 gccatagccg gccccgggaa gaaggcgatc cccgcttcca gcacggagaa accccaaaca
      361 gtggtcagga acaggacccc gccgaggagt atcgcggcga aggccaccat gaagatcagc
      421 gcggtcagac aggccatcgc gaagggcctg gccttgaaga gatccacgtc cagggtcggg
      481 atccagcgat cggccggccg tcgggagcgc agaaaggtca gtcccagcat gaccaggccg
      541 gcgaggatcg cgacgacggt ccggatgctg ccccaaccat ggtccggcgc ttcgaccagt
      601 ccccagcaga gcaccccgat gccgaggatc atcagggcca tgccggccgg gtcgggccac
      661 ctggtttcgc gtggatcctt cgactcgacc agcagccgcg tcccgaaata ccaggctgcg
      721 agcgaaagcg gcaggttcac gaggaagacc catcgccagg agatttcgac cagcaaacca
      781 ccgatcggcg gaccgagagc gccggccgcg ccgcccgccg ccgcccagac gccgatcacg
      841 accggccagc gctccgccgg aaaggcgtgg agcagcaggg ccagcgaagt ggacatcagc
      901 atcgccgcgc cgatcccctg aatgacccgg aaggcgacca gggtctcgac cgaccaggcc
      961 gcggcgcagg ctccggaagc aagacagaag atcaacaccc cggtcgtgaa gattcgcttg
     1021 cgcccgaccc ggtcggccag ccggccaaag ggcatgagca aagcggcgaa gacgatcacg
     1081 tacccgttga ggacccagga aagcgagccg atcgaactgc cgggaaagtc ggcttcgatg
     1141 gccgggaagg cgatgttgac gatgaacatg tcgaggctgg ccagggccac cgccgctgcc
     1201 acgaccgcca gcaccttgcc gaggacctcg ggccggtagg ccgcttccgc ctccgccatc
     1261 tccttcggca ccgggcctgc ggccggggtc ggagcaccga ccaggatttc ctcttccact
     1321 gcctcgaact ttccggtcac ggtgtccgac atgttcactt gtcccgagtg agtatcttca
     1381 tgcgactgac cctaacacaa tcgggtcgca tgatgcgact gacccgctac aatcggtcca
     1441 tgctcgatcg cgagtacgac aaccaggtct gttcgatcgc ccgttcgctc gaggtggtcg
     1501 gtgagcgctg gtcactcctg atcatcagga acgtcggcct cggcctgaac cgtttcgagc
     1561 cgctccgcga gaacctcggg atcactcgca gcgtcctgac cacgcgcctc aacaccctga
     1621 tcgaatacgg gatcctcgag aagcgccagt acagcgagaa gcccccgcgc ttcgaatacc
     1681 acctgaccca aaagggccgc gacctctccc ccgtcctgct gcagctgatg tggtggggtg
     1741 acgagtacta ccccgagccc ggcggccctc cgaccatcgc cgtccaccgc acctgcggcg
     1801 gcaacctcga ccaccacatg gtctgcgaaa agtgcggcga atcagtcgag ccggaccggg
     1861 tctacatgaa acccggcccc ggccgccgcc ggcaggacct ccccaaaatc cccgcctgaa
     1921 cgccacacca gcccgtcagg cccggaggtg ccgcaaatcc agcaccgtga tgggcctgtt
     1981 cgtgcgacac ctccacgacc agcccgaccc gagcaccgcg agatcccact ttgcgccaca
     2041 taccggcaca aagtgggcat tcggtgattc caaccccacc taatcccccg gtgggtcgga
     2101 tttccgtcac ccgccccgaa aatccgaccc actggcacct ccggaactga ttcgcaagtc
     2161 tcgtgggtcg gattttcggc ccgactgcgg agaatccgac ccacctgggc ggtgcagggc
     2221 aggtgaaggg gaagttgaag gaacccaggg tccgggctgg ctccggggcc gaattcgcgt
     2281 cctggttcag ggcggcaccg tcccccgagt cacggtcggg aatcgatcga gatgctgttt
     2341 gtcggcgata tttgccgaca aacagcatct cgcgaaatgc caacaccacc aa
//
LOCUS       DAOQFI010000273         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24719_length_2388_cov_3.292756, whole genome shotgun sequence.
ACCESSION   DAOQFI010000273 DAOQFI010000000
VERSION     DAOQFI010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24719_length_2388_cov_3.292756"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLE93_05565"
     CDS             complement(<1..805)
                     /locus_tag="PLE93_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNH86550.1"
                     /translation="MIELQSHSVVSDGQLEPAGVVEEAAKAGVEVLALTDHDGVAGVP
                     EAVEAGAQLGVEIVPAVEMSSVHQYAEDLHICGYWVDVEKITPACQRAQEERRNRAKE
                     IVQNLRDHGFDLTFEDAVREAGAADAIGRPHIAKAAGATGNLGPFFEEWLVPGAKAFV
                     SRKWPDANEACALIREAGGVPVIAHPYWDIKVPEQVEELIRALDIGGIEVFYPSHTRA
                     QTKHLLDLANELGVTATASSDYHGPTHKTFSRFGAYQTYDLGQPEVPPRP"
     gene            complement(998..1486)
                     /locus_tag="PLE93_05570"
     CDS             complement(998..1486)
                     /locus_tag="PLE93_05570"
                     /inference="COORDINATES: protein motif:HMM:NF037405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNH86551.1"
                     /translation="MALLDFAVSAPDTDVKFAFLELCRLRLFGEKDVQFCFRRSVGKR
                     GALKVRPLLGLWVPELNRIKSVLEGLFLLSWVPRRWKLPEVNVKIFGREVDFLWRKEG
                     LVLELDGDAFHTDPIARQRDLEKTRFLESKGLTVIRITWKEFMEDPDGVVERIARELG
                     LI"
     gene            2226..>2388
                     /locus_tag="PLE93_05575"
     CDS             2226..>2388
                     /locus_tag="PLE93_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNH86552.1"
                     /translation="MFSKILIANRGEIAIRVARACKEMGITSVAVYSEADRDAPHVKA
                     CDEAFLIGPA"
BASE COUNT          434 a          808 c          714 g          432 t
ORIGIN      
        1 ccggcctggg cgggacttcg ggctggccca ggtcgtaggt ctggtaggcg ccgaagcggc
       61 tgaaggtctt gtgggtgggg ccgtggtagt cggatgaggc ggtggcggtc acgcccagct
      121 cgtttgcgag gtccaggagg tgcttggtct gggccctggt atgagacggg tagaagacct
      181 cgatgccacc gatgtcgagg gcgcggatca gctcctcgac ctgctccgga accttgatgt
      241 cccagtaggg gtgggcgatc accggcacgc cgccggcctc gcggatcagg gcgcaggcct
      301 cgttcgcgtc cggccacttg cgggagacga acgcctttgc ccccggcacc agccactcct
      361 cgaagaatgg gccgaggttg ccggtcgccc cggccgcctt ggcgatgtga ggtcggccga
      421 tcgcgtcggc ggcgcccgcc tcgcggaccg catcctcgaa ggtcaggtcg aagccgtggt
      481 cgcgcaggtt ctggacgatc tccttcgccc tgttccgccg ctcttcctgg gcccgctgac
      541 aggccggggt gatcttctcc acgtcgaccc agtagccgca gatgtgaagg tcctccgcgt
      601 actggtgaac cgaggacatc tcgaccgccg gcacgatctc cactccgagc tgcgccccgg
      661 cctcgaccgc ctccggcaca cccgccaccc cgtcgtgatc ggtcagggcg agcacctcga
      721 ccccggcctt cgccgcctcc tcgactaccc ccgccggttc aagctggcca tcagagacaa
      781 cggaatgaga ctgaagttcg atcatccccc cgaatcctaa gctccacccc tgaattccgc
      841 tccgccccac aagcccccac cgtttcaacc cctcctcccc tcaatccccc tcgaactccc
      901 tccccaaaaa tcgagaaggc aaaagtagcc atatagcgct ccattcagcc ttctcggctc
      961 atcacccagc cgctcgacca ctttgctggc gggactctca gatcagcccg agttcgcggg
     1021 caatccgctc gaccacaccg tctggatctt ccatgaactc cttccaggtg atccggatga
     1081 ccgtaagtcc cttgctttcc aggaaacggg tcttctccag gtcccgttgc ctggcgatgg
     1141 gatcggtgtg gaaggcatct ccgtcgagct ccagcacgag accttctttt cgccaaagga
     1201 agtcgacctc tcgcccgaag atcttcacgt tcacctccgg cagcttccac cgtcgcggca
     1261 cccacgacag gagaaacagc ccctcgagta cggacttgat ccggttcagt tccggcaccc
     1321 acaacccgag caacggcctc acctttagcg cgcctcgttt tcccactgac cggcggaagc
     1381 agaactgcac gtccttttcg ccaaacaacc ggagtcggca caactccagg aaagcgaatt
     1441 tcacgtctgt gtcaggagca cttacggcaa agtcgagcag ggccaaagct gctcgcacga
     1501 ccgcaaggcc gcctctctcg cgcaggtcac ccgggtcgaa ccgtcggcgg accacataga
     1561 cccaggtgtt cctgagagcg ggcgacagcc ctcggtgcct gccagcgcgg tctctggatg
     1621 tagccacgtg gatcactggc gggatcggag tcaagaggcg cgccgccccc aggagctcca
     1681 gggcactccg tgaggtcagg gcagaaccgg gacccgcaac caggagagcc gcccgccagg
     1741 ccgactctct ggtctctatg tcccgcccgt aggcatagat gccgtggaag agtttggtca
     1801 accgcctgcc tctgagccaa ttcttgatcc tggacggact gaatccatga cgaatgagtt
     1861 catccctccc ggcaacatga cggcgccggg acatgaactg ccaaagccgg tcaagctcga
     1921 tggcgctgga tggactttcc ccggagaccg aattcatcgg cccaagcctg cccgagaaat
     1981 gctcacacgt ggctctataa gggtgacaat tgtgcaacgt cccgggagcc tgaaacgccc
     2041 gcgaagcctg ccttgaactc gccggttcga gccgccaccg ccgagaaggc taaatggagc
     2101 gctatatggc cacttttgcc ttctcgattc tggggctggc ttggatcgga tggctctgcg
     2161 cgggctggct gggagggggc gggggcaaag ccgggaccgg ggcggataaa tagactgccc
     2221 gggctatgtt ttcgaagatc ctgatcgcca atcgtggcga aatcgctatc cgtgtcgccc
     2281 gggcttgcaa ggagatgggg attacctccg tcgccgttta ttcggaggcg gatcgggatg
     2341 ctccgcacgt caaagcctgt gacgaagctt tcctgatcgg accggcgg
//
LOCUS       DAOQFI010000274         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_24880_length_2379_cov_3.290878, whole genome shotgun sequence.
ACCESSION   DAOQFI010000274 DAOQFI010000000
VERSION     DAOQFI010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24880_length_2379_cov_3.290878"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLE93_05580"
     CDS             complement(<1..1092)
                     /locus_tag="PLE93_05580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH86553.1"
                     /translation="MNGRTVKYESLRIRPFGMHPAHRGYRDLGDYRRNQVRYGIAFAT
                     ITAICFAATLVSSEDVQWGLYLGGWALALACVGLAWANPALWLLAPFGALGGAILVRA
                     GTDGLDAGIGPLLLIPMLAVAVYGSRRALIAMIACVAGAVIIGQLVWDGGNSEMSPIW
                     RQDIVLAMLATALGVAMQDLVTRMRSERNLAEERGRIIEELALTDPLTGIDNRRGWER
                     MIQRSVGQARRHGQPLCLAMLDLDHFKLYNDTFGHQAGDDFLRRVALDWEERTRIEDH
                     VARYGGEEFVVTLPNTGLAGAKVIVERLRTGLPLPLEDRESTITCSAGIAEWDGSETV
                     RELVARADQALYLAKEGGRDRTVTVEASAR"
     gene            1136..1708
                     /locus_tag="PLE93_05585"
     CDS             1136..1708
                     /locus_tag="PLE93_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86554.1"
                     /translation="MFKAPARADNIGSLVAKDRSKEILKEIRNLREELSARLDAIEAR
                     AGAGRFPESSSGFPDSAAPGASLPGIPVSNSGIPDPAPAVNDNFELPTGGEVRVIVRP
                     LHDLSLARVVEDALANTEGVNEASLRELRGDSATIDTKVDEGVSLISSLRRKLPVAFD
                     VTESSSDSVTIALAQPMGGRESGVAAPTGL"
     gene            1705..1962
                     /locus_tag="PLE93_05590"
     CDS             1705..1962
                     /locus_tag="PLE93_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86555.1"
                     /translation="MNSGGGVFGALIFVIGILLAFAVLFGFFLIVPFFVFLAGIVVML
                     VSDRRRGGENGNGNGNGNGNGTRTGTVEVVETTETVREVKS"
     gene            1962..>2379
                     /locus_tag="PLE93_05595"
     CDS             1962..>2379
                     /locus_tag="PLE93_05595"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86556.1"
                     /translation="MATAVAQKLLVRDSTSGVSERMVTLSSAVAHLLPVEICRRYHIV
                     PIEFDRGLITMAGPVSFNPAADRAVREQTGYETRWLFADQTELDLAIDRLFAGNGRPE
                     GTEFSADDLLRHGLAVPPRLGESLASRASSWGSCRVD"
BASE COUNT          404 a          781 c          770 g          424 t
ORIGIN      
        1 gcgggccgaa gcttctaccg tgacggtccg gtcccggccg ccttccttgg ccaggtagag
       61 cgcctggtcg gcccgggcca ccagttcccg caccgtttcc gacccatccc actcggcgat
      121 gccggccgag caggtgatcg tgctctcgcg gtcttcgagc gggagcggca ggccggtgcg
      181 gagccgctcg acgattacct tggccccggc cagcccggtg ttcggcaggg tcaccacgaa
      241 ctcctcgccg ccgtatcggg cgacgtggtc ttcgatcctg gtccgctctt cccagtcgag
      301 ggcgaccctt ctgaggaaat cgtccccggc ctgatggccg aaagtgtcgt tgtaaagctt
      361 gaagtggtcg aggtccagca ttgccaggca aagcggctgg ccgtgccgcc gggcctgacc
      421 gaccgaacgc tggatcatcc gctcccagcc gcgccggttg tcgatcccgg tcaggggatc
      481 ggtcaacgcc agctcttcga tgatccggcc acgctcctcg gcgaggttgc gctcgctgcg
      541 catacgggtg accagatcct gcattgccac accgagtgcc gtcgcgagca tcgccagcac
      601 gatgtcctgc cgccagatcg gactcatctc ggagttgccg ccgtcccaga cgagctggcc
      661 gatgatcacc gcgccggcca cacaggcgat catcgcgatc agggcgcgcc gtgatccgta
      721 cacggcgacc gcgagcatcg ggatcagcag cagcggcccg atgccggcgt cgagaccgtc
      781 ggtccccgcc cgcacgagaa tcgcaccgcc gagcgccccg aacggcgcga gcagccagag
      841 ggccggattc gcccaggcca ggcccacgca ggcaagggcg agtgcccagc cgcccaggta
      901 gagcccccac tggacgtcct cagaggaaac cagggtcgct gcaaagcaga ttgcagtgat
      961 cgtggcgaag gcgattccgt agcgaacctg attgcgacgg taatccccga ggtcccggta
     1021 gccgcgatgg gccgggtgca ttccgaacgg acggattctc aggctttcgt acttgacggt
     1081 tctcccgttc atcacctata gtgggccact cactggcggc aggacttacc attcggtgtt
     1141 caaagctcct gcacgtgccg ataatatcgg gagcctggtg gctaaagacc gttcaaaaga
     1201 aattctcaag gaaatcagga atctaaggga ggaactctcc gcccggctcg acgccatcga
     1261 ggcgcgcgcc ggagccggtc gttttcctga atcttcttcc ggttttcctg attcggcggc
     1321 gccaggcgct tcccttcccg gaataccggt gagcaactcc ggcattccgg atccggcacc
     1381 tgcggtcaac gacaacttcg agttgccgac cggtggagag gtgcgggtga tcgtccggcc
     1441 gcttcacgat ctgagccttg cccgcgtggt cgaagacgcg ctggccaata ccgaaggtgt
     1501 gaacgaggct tcgctgcgcg aacttcgcgg cgactcagcc accatcgaca ccaaggttga
     1561 cgagggcgtt tcgctgatct cttcgcttag acgcaaactg ccggtggcat tcgacgtcac
     1621 cgagtcttcc agtgactcgg tgactatcgc gctggcccaa ccgatgggcg ggcgcgagag
     1681 cggcgtggcc gctcccacgg gtctgtgaac agcggcggcg gagttttcgg tgcgctgatc
     1741 ttcgtgatcg gcattctgct tgcttttgcg gtgctgttcg gcttcttcct gatcgtcccg
     1801 ttcttcgtct ttttggccgg aatcgtcgtg atgctggtca gtgaccggcg gcgcggcggt
     1861 gaaaatggca acgggaacgg caacggcaac ggaaacggca ccagaaccgg gaccgtcgag
     1921 gtggtcgaaa ccaccgagac ggttcgggag gtcaagtcct gatggccacc gccgtcgcac
     1981 agaaactgct ggtccgcgat tccaccagcg gcgtcagcga gcgcatggtc acgctcagct
     2041 cggccgtggc acatctgttg ccggtcgaga tctgccgccg gtaccacata gttccgatcg
     2101 aatttgaccg cggactgatc accatggccg ggccggtttc cttcaatccg gcggccgacc
     2161 gggcggtgcg cgagcagacc ggttacgaaa cccgctggct cttcgccgac cagaccgagc
     2221 tcgacctggc catcgaccgg cttttcgccg gcaacggacg gccggaaggg accgagttct
     2281 cggccgatga cctgctccgg cacggactgg ccgttccgcc ccggctgggc gagagcctgg
     2341 ccagcagggc ttcgagctgg ggcagctgca gggtggacg
//
LOCUS       DAOQFI010000275         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25044_length_2371_cov_3.131693, whole genome shotgun sequence.
ACCESSION   DAOQFI010000275 DAOQFI010000000
VERSION     DAOQFI010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25044_length_2371_cov_3.131693"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1254
                     /locus_tag="PLE93_05600"
     CDS             136..1254
                     /locus_tag="PLE93_05600"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNH86557.1"
                     /translation="MKIGVPRESAEGENRVALVPESVKSLLKKEGVEIVIESGAGDVA
                     GHPDSQYEEAGAKVGSHADALAADLVLHVAPLSSAQIGELKQGQYLISHLSPLTSAET
                     NNALASAGVTALAMELIPRTTRAQAMDALSSQATAAGYAATLIAARESGSFWGMLTTA
                     AGTIRPARVLVLGAGVAGLQAIATAKRLGAIVTGFDIRRAAWEQIASLGGRPLELDFI
                     PDAEDEGGYARPLTDEENQQVRDAIDANAAKQDVIITTAAIPGRAAPTLITAAGVANM
                     GPGSVILDLAAETGGNCELTEPGQTVVKDGVKIVGPKNLASELPGHASQLYAKNLENL
                     LGLMITEEGGLTLNFEDDIIDAATITHQGEIRGERTAR"
     gene            1254..1601
                     /locus_tag="PLE93_05605"
     CDS             1254..1601
                     /locus_tag="PLE93_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNH86558.1"
                     /translation="MNLVTEITILVLAVFVGFEVISKVPTTLHTPLMSQTNAIHGIVL
                     LGGLIVVGYSDDFLGQLIGFIAIVFGTINIVGGFMVTDRMLEMFGPKKKKPEPAAEPA
                     ATDSASDSTEAGS"
     gene            1598..>2371
                     /locus_tag="PLE93_05610"
     CDS             1598..>2371
                     /locus_tag="PLE93_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNH86559.1"
                     /translation="MSPLLAALAPDSSLVAILYIVSFSLFIIGIKQGTHPTTARRGNM
                     IAAVGMAIAVITTLCLKGIGNWGIIIAGILVGTIVGAVASKRVKMTEMPQMVALYNGL
                     GGGAIALIAWSEYRHWVHLGDVLADKASFSHKFLLDFDNLTFLVGKNFSVSASVPLEV
                     MIPTLLAMVVGSISFWGSNVAFAKLQELIPGQPMGLPGQKIINGLLLAALAVGGVILA
                     INNDVNDLSQPLFIAMLVVAALLGLGVVMPIGGADMPVVI"
BASE COUNT          410 a          824 c          732 g          405 t
ORIGIN      
        1 cagatgcggc acaccgtcga gcggctggcc gccctgacgc aggactgacc aagaggatct
       61 gatccggccg gcggctgtcc gccggtcggg aatcccctct gcggctaatt cccataatct
      121 gagtcggcct tgtccatgaa gatcggtgta cccagagaat ctgctgaagg cgagaaccgg
      181 gttgccctgg ttcccgagtc ggtcaaatcg ctcctgaaaa aggaaggcgt cgagatcgtc
      241 atcgagtccg gagccggcga tgtcgccggg catccagaca gccagtacga ggaagcggga
      301 gcgaaggtcg gttcgcatgc cgacgcgctc gcggccgatc tcgtgcttca cgtcgctccg
      361 ctttcgagcg cccagatcgg cgagttgaag cagggccagt acctgatcag ccatctctcg
      421 ccgctgacct cggcggagac caacaacgcc ctcgcctcgg ccggggtcac cgccctggcg
      481 atggaactga ttccgcgcac cacccgcgcc caggcgatgg acgcactttc ctctcaggcg
      541 accgcggccg gttatgccgc gactctgatc gccgcgcgcg agtcgggcag cttctggggc
      601 atgctcacca ccgctgccgg cacgatccgt ccggcccgcg tcctggtgct cggggccggc
      661 gtcgccgggc tgcaggcgat cgccacggcc aagcgtctcg gggcgatcgt caccggtttc
      721 gacatccgcc gcgccgcctg ggaacagatc gcctccctgg gcggtcgccc gctcgagctt
      781 gacttcattc ccgacgccga ggacgagggc ggctacgccc gtcccctgac cgacgaggaa
      841 aaccagcagg tccgcgacgc gatcgacgcg aacgcggcca agcaggacgt gatcatcacc
      901 accgccgcga tccccggtcg cgccgcgccg accctgatca ccgcggccgg tgtcgccaac
      961 atgggtcccg gatcggtgat cctcgacctg gccgccgaga ccggcggcaa ctgcgagctg
     1021 accgagcccg gccagaccgt ggtcaaggac ggagtcaaga tcgtcggccc gaagaacctc
     1081 gcttcggagc tgcccggtca cgcatcccag ctttacgcca agaacctcga gaacctgctc
     1141 ggactgatga tcaccgagga gggcggtctc accctgaact tcgaggacga catcatcgat
     1201 gccgcgacga tcacccacca gggtgaaatt cgcggagaga ggaccgcccg ctgatgaacc
     1261 tcgtaaccga gataaccatc ctcgtgctgg cggtcttcgt cggcttcgaa gtgatctcca
     1321 aggttccgac cacgctgcac acgccgctga tgtcgcagac caacgcgatc cacgggatcg
     1381 tcctgctggg cggcctgatc gtggtcggct actccgatga cttcctcggc cagctgatcg
     1441 gcttcatcgc gatcgtcttc ggcacgatca acatcgtcgg cggcttcatg gtcaccgacc
     1501 gcatgctgga aatgttcggg ccgaagaaga aaaagcccga acccgcggct gaaccggcag
     1561 ctaccgattc tgcgtcggac tcgacggagg ccggctcgtg agtccgcttc tcgctgcgct
     1621 ggcgccggat tcgtcgctgg tcgcgattct ctacatcgtc agcttctcgc tgttcatcat
     1681 cgggatcaag cagggcaccc acccgaccac cgcccggcgc ggcaacatga ttgccgccgt
     1741 cggtatggcg atcgcggtca tcaccacgct ctgcctgaag gggatcggca actggggcat
     1801 catcatcgcc ggcatcctgg tcggcacgat cgtcggcgcg gtcgcctcga agcgggtcaa
     1861 gatgaccgaa atgccgcaga tggtcgcgct ctacaacggc ctcggcggcg gcgcgatcgc
     1921 cctgatcgca tggtccgagt accggcactg ggtccacctc ggtgacgtgc tcgccgacaa
     1981 ggccagcttc agccacaagt tcctgctcga tttcgacaac ctcaccttcc tcgtcgggaa
     2041 gaacttcagc gtcagcgctt cggtgccgct cgaagtgatg atcccgaccc tgctcgccat
     2101 ggttgtcggc tcgatttcct tctggggctc caacgtcgcc ttcgccaagc tgcaggaact
     2161 gatcccgggc cagccgatgg gcctgccagg ccagaagatc atcaacggcc tgttgctcgc
     2221 cgcgctcgcg gtcggcgggg tcatcctcgc catcaacaac gacgtcaacg acctgagcca
     2281 gccgctcttc atcgccatgc tggtcgtggc cgccctgctc ggcctcggcg tggtcatgcc
     2341 gatcggcggg gccgacatgc cggtcgtgat c
//
LOCUS       DAOQFI010000276         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25115_length_2368_cov_2.347601, whole genome shotgun sequence.
ACCESSION   DAOQFI010000276 DAOQFI010000000
VERSION     DAOQFI010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25115_length_2368_cov_2.347601"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLE93_05615"
     CDS             <1..255
                     /locus_tag="PLE93_05615"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNH86560.1"
                     /translation="RTVPVQPDDSVINAAASADAMTFTSASTVSSLLAAVPGGLPGPV
                     NVSMGPITSEALREAGLEVAIEAGSGSLEGLVNATAKVLS"
     gene            252..1295
                     /gene="hemE"
                     /locus_tag="PLE93_05620"
     CDS             252..1295
                     /gene="hemE"
                     /locus_tag="PLE93_05620"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNH86561.1"
                     /translation="MSQAAEATLLVRALRREPVERKPVWFMRQAGRSLPEYRELREGA
                     PMLEMCRTPDLCAEVTLQPVRRHGVDAAIFFSDIVTPLEGIGIDVEIQSGVGPVIAEP
                     FRSEADLARLRPLEPDTDLPEIAEAIRIVTAELGDTPLIGFAGAPFTLASYLVEGRPS
                     KSQEMARTMLPEEPGLFRNLLERLADIAVASLRSQVEAGVQAVQVFDSWAGSLSEATY
                     REAVLPVSQRLFAEIEDLGVPRIHFAVNAGHLMEAMAEAGSDAMGVDWRTPLSEARRR
                     LGEGVALQGNLDPTVPLAGPEVLERETRNLLADAPERGHVFNLGHGVLPSTDPGMLTE
                     LVGLVHEITEKRN"
     gene            1292..2221
                     /gene="hemH"
                     /locus_tag="PLE93_05625"
     CDS             1292..2221
                     /gene="hemH"
                     /locus_tag="PLE93_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNH86562.1"
                     /translation="MSDEAAKTGVLVMAYGTATGPDDVEAYYTHIRHGRPPEPEALKE
                     LQERYEMIGGSPLLGITQAQAEGIAAALERSNPGDFTTAMGQKHSAPFIEDAVRELLD
                     RGVERIVGIVLAPHFSTMSIAQYRSRAEEAAAEAGVPVTVIESWHEEPGYLEFLNQAI
                     TDAYEALPAEARESVELAVTAHSLPVAILERGDIYAEELERTRENLAGRPEFTGTTLA
                     WQSAGRTDAEWIGPDICEIMPGLKEAGRTAVVVCAAGFVSDHLEVKFDIDYEAKQAAD
                     EAGLALTRTAMPNDDPGFCATLAGIVAREAGKS"
BASE COUNT          384 a          788 c          837 g          359 t
ORIGIN      
        1 cggaccgtcc ccgtccagcc ggatgattcg gtgatcaacg cggcggcaag cgccgacgcg
       61 atgaccttca cttccgcctc gaccgtttcc agtctactgg ccgcggtgcc tggcggcctg
      121 cccggaccgg tcaacgtttc gatggggccg attacctccg aggcacttcg cgaggccggg
      181 ctggaagtag cgatcgaggc cgggtcgggc agcctcgaag gcctcgtgaa cgcaaccgca
      241 aaggtccttt cgtgagtcag gctgccgaag ctacgttgct ggtccgggca cttcgccggg
      301 aaccggtgga gcgcaagccg gtctggttca tgcggcaggc cggccgctcc ctgccggaat
      361 accgcgaact ccgggaaggg gccccgatgc tggaaatgtg ccggacgccg gatctctgcg
      421 ccgaggtcac cctgcagccg gtccggcgcc acggggttga cgcggcgatc ttcttctccg
      481 acatcgtcac cccgctggag gggatcggca tcgacgtgga gatccagtcg ggggttggcc
      541 cggtgatcgc ggaaccattc aggagtgaag ccgacctggc ccggttgcgg ccgctggagc
      601 ccgatacaga cctgccggag atcgcggaag cgatccggat cgtcaccgcc gaactcggtg
      661 acacgccgct gatcgggttt gccggggcgc cgttcaccct ggccagctac ctggtcgagg
      721 gccggccctc gaagtcacag gaaatggccc ggaccatgct gccggaggaa ccggggctgt
      781 tccggaacct gctcgaacgc cttgccgaca tcgcggtcgc ttcgcttcgc tcgcaggtgg
      841 aggccggggt gcaggcggtg caggtcttcg actcctgggc cggctcgctt tccgaggcga
      901 cttaccggga ggccgtcctg ccggtctcgc aaaggctctt cgccgagatc gaggatctgg
      961 gggtgccgcg gattcacttc gcggtcaacg ccggccacct gatggaggcg atggccgagg
     1021 ccggctccga cgcgatgggg gtggactggc ggaccccgct ctccgaggct cgccgccggc
     1081 tcggtgaggg cgtcgccctg cagggcaacc tcgatccaac cgtgcccctg gccgggccgg
     1141 aagtgctcga gcgcgagacc cgcaacctgc tggccgacgc tcccgagcgg ggtcatgtct
     1201 tcaatctggg tcacggagtc ctgccttcga ccgacccggg catgctgacc gagctggtcg
     1261 gcctggttca cgagatcacg gagaagagga attgagcgac gaagcagcga agaccggagt
     1321 cctggtcatg gcctatggaa ccgcgaccgg gccggatgac gtcgaggcct attacacgca
     1381 catccgccac ggccgcccgc ctgagccaga ggccctgaag gagcttcagg aacgctacga
     1441 gatgattggc ggatcaccgc tcctgggcat cacccaggcc caggccgaag gcatcgccgc
     1501 cgccctcgag cggtcgaacc cgggcgattt cacgaccgcc atgggccaga agcattcggc
     1561 ccccttcatc gaggatgccg tgcgggagct gctcgaccgg ggagtggagc ggatcgtcgg
     1621 catcgtcctg gctccgcact tctcgaccat gagcatcgcc cagtaccgca gccgggccga
     1681 ggaagccgcg gcggaagccg gggtgccggt caccgtgatc gaatcgtggc acgaggaacc
     1741 gggctacctc gagttcctga accaggcgat caccgacgcc tacgaggcgc tgcccgcgga
     1801 agcccgtgaa tcggtcgagc tggcggtgac cgcccacagc cttccggtgg cgattctcga
     1861 gcgaggcgac atctacgccg aggagctgga gaggacccgc gagaacctcg ccgggaggcc
     1921 ggagttcacc ggcacgaccc tggcctggca gagtgccgga cgcaccgacg ccgagtggat
     1981 cggcccggac atctgcgaga tcatgcccgg cctgaaggaa gcggggcgga cggccgtggt
     2041 ggtctgcgcc gccgggttcg tttccgacca cctcgaggtc aagttcgaca tcgactacga
     2101 ggcaaagcag gcggccgacg aagcgggact ggccctgacc cgcaccgcca tgcccaatga
     2161 cgatccgggc ttctgtgcca cgctggccgg gatcgtcgcc cgggaggccg gcaagtcttg
     2221 aagcgggcgg tcgtcgtcgg agccggcatc gccgggctga ctgcggcctg gcaactcagc
     2281 cgatccggcc acgaagtgac ggtgctggac cgggccggcc ggaccggcgg ccggatcctt
     2341 ccggctgaat tctcggggcg gctggttg
//
LOCUS       DAOQFI010000277         2360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25266_length_2360_cov_3.168764, whole genome shotgun sequence.
ACCESSION   DAOQFI010000277 DAOQFI010000000
VERSION     DAOQFI010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25266_length_2360_cov_3.168764"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLE93_05630"
     CDS             complement(<1..513)
                     /locus_tag="PLE93_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNH86563.1"
                     /translation="MSILLNKDTTFIVQGITGREAVNLTKECLDYGSKVVGGVTPGRK
                     GREVHGVPVFDTVEQAVENHGSQIDGSIVTVPPGFTKDAVIEAIDNGIKLIVVVTERI
                     PRGDVAEMVEFADERGARIIGPNCLGLIAPGVAKMGGIGGPAKDAAKAYVAGSVGVMS
                     RSGGMTTEISS"
     gene            complement(513..1727)
                     /locus_tag="PLE93_05635"
     CDS             complement(513..1727)
                     /locus_tag="PLE93_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNH86564.1"
                     /translation="MIFYEYEARKIVELAGIPVTKYGFCQTPEEARKIAEEIGGPTVI
                     KSQVLTGGRMKAGGVKFADTPDEAAQHAEDILKLEINGHMPVGVLVDPKAEVKQEYYA
                     SVVWDGTAKKPLMLFSDMGGIDIEQVAEEHPDHVGRGHISNLLPVGDYEAKQVIAATG
                     VTGSQLNRATPILAKLARLFRENDMILAEINPLAELTDGTFVALDAHMEMESEAEGRQ
                     KKLLRKELGIAEDDNRDPYVPSEFEKNVKEIDAADHRGVIQGKDHGFEGNLGLVIGAG
                     GGSLTLTDAVRSQGGKPANYSEIGGNPSVAKACGLAKEVLKKDGVEKIAVMMSIVSNT
                     RVDIVARGVIKACLELGKDPGETIAIFRIPGAWEDEGNKILDHYGVEYCDRSVSLWDA
                     AGRAVAKIQEQN"
     gene            1824..2123
                     /locus_tag="PLE93_05640"
     CDS             1824..2123
                     /locus_tag="PLE93_05640"
                     /inference="COORDINATES: protein motif:HMM:NF025590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HNH86565.1"
                     /translation="MLVRPLFLLAGPFLAAMILAGCGAGDVIDDQKTEIAVRFDVQDA
                     TGEPVRSVTCPSGVPVSVGTRFTCHVVAKSGDDAVAELEVTSDDGDLRMLSLTAP"
BASE COUNT          357 a          752 c          774 g          477 t
ORIGIN      
        1 ggaggagatc tcggtcgtca tgccgccgga gcgggacatg accccgaccg agccggcgac
       61 gtaggccttg gcggcgtcct tggcggggcc gccgatgccg cccatcttgg cgacgccggg
      121 ggcgatcagc ccgaggcagt tggggccgat gatgcgggcg ccgcgctcgt cggcgaactc
      181 gaccatctcg gccacgtcgc cgcgcgggat gcgttcggtg acgacgacga tcagcttgat
      241 cccgttgtcg atcgcctcga tcaccgcgtc cttggtgaag ccggggggga cggtgacgat
      301 cgagccgtcg atctggctgc catggttttc aaccgcctgc tcgaccgtgt cgaagaccgg
      361 cacgccgtgg acttcgcggc ccttgcggcc gggggtcacg ccgccgacga ccttcgagcc
      421 gtagtcgagg cattccttgg tcaggttgac cgcttcgcgg ccggtgatgc cctggacgat
      481 gaaggtggtg tccttgttca ggagaattga cattagttct gctcctggat cttggcgacg
      541 gctcgtccgg cggcatccca gagggagacc gaacggtcgc agtattcgac gccgtagtgg
      601 tcgaggatct tgttgccctc gtcttcccag gcgcccggga tgcggaagat ggcgatggtc
      661 tcgccgggat ccttgccgag ttcgaggcag gccttgatca ctccgcgggc cacgatgtca
      721 acccgggtgt tggaaacgat cgacatcatc acggcgatct tctcgacgcc gtccttcttg
      781 aggacttcct tggcgagacc acaggccttg gcgaccgagg ggttgccgcc gatctccgag
      841 tagttggccg gcttgccgcc ctgggagcga accgcgtcgg tcagagtcag cgaaccgccg
      901 ccggcgccga tcaccaggcc gaggttgcct tcgaagccgt ggtccttgcc ctggatcacg
      961 ccgcgatggt cggccgcgtc gatctccttg acgttcttct cgaactcgga cgggacatac
     1021 gggtcgcggt tgtcgtcctc ggcaatcccg agttccttcc gcaggagctt cttctggcga
     1081 ccctcggcct cggactccat ctccatgtgg gcgtcgagtg cgacgaaggt cccgtcggtc
     1141 agctcggcca ggggattgat ctcggccagg atcatgtcgt tttcacggaa gaggcgggcc
     1201 agcttggcga ggatcggcgt tgcccggttc aactggctgc cggtcacgcc ggttgcagcg
     1261 atgacctgct tggcctcgta gtcaccgacc ggaagcaggt tcgagatgtg accgcggccc
     1321 acgtggtccg ggtgctcctc ggcgacctgc tcgatgtcga tgccgcccat gtcgctgaac
     1381 agcatcagcg gcttcttggc agtgccgtcc cagaccaccg aggcgtagta ctcctgcttg
     1441 acctcggcct tcgggtcgac caggacgccg accggcatgt ggccgttgat ctcgagcttg
     1501 agaatgtcct cggcgtgctg cgcggcctca tccggggtgt cggcgaactt tacgccgccc
     1561 gctttcatgc ggccaccggt caggacctgg gacttgatca cggtcggacc gccaatttcc
     1621 tcggcgatct tccgtgcctc ttcgggggtt tggcagaagc cgtacttggt gaccggaatt
     1681 ccggccagct cgacgatctt tcttgcctcg tattcgtaga agatcattgc ggcgaaaatc
     1741 tattccagaa ccgggcgacc ggttattccg attgagtcgc taggaatcgg cggcgcgacc
     1801 ggacgctatg tagtgtgcgg cccgtgttgg tccgtcctct cttcctgctc gccggcccgt
     1861 ttctggccgc catgatcctt gccggctgcg gagccggtga cgtgatcgac gatcagaaga
     1921 ccgagatcgc ggtccggttc gacgtgcagg acgccaccgg ggagccggtg cgctcggtga
     1981 cctgcccgtc cggggtgccg gtctcggtcg gcacccgctt cacctgtcac gtggtggcga
     2041 agtcgggcga tgacgccgtg gccgaactcg aagtcacctc cgatgacggc gatttgcgga
     2101 tgctgtcgct caccgccccc tgattcggcc aaaccagtgg tggtaaccgg cggagtcgtc
     2161 tgtgaatcat gttcggtagt tatccgcaga ccctcgctgg tcagaccgca attcgcactg
     2221 atctccgtca caattccgta accggctaaa tccgcgccgc cctctccttc gcaggcgcgt
     2281 tgaccaagga gttgaagagc gccagtgagt gaaacgaccc aggtgaccgc cccgaccaag
     2341 aacgccaggc tgcttgcctg
//
LOCUS       DAOQFI010000278         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25358_length_2354_cov_3.530666, whole genome shotgun sequence.
ACCESSION   DAOQFI010000278 DAOQFI010000000
VERSION     DAOQFI010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25358_length_2354_cov_3.530666"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="PLE93_05645"
     CDS             <1..150
                     /locus_tag="PLE93_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH86566.1"
                     /translation="DDNPQTAQQYGVLSIPTMILLKDGALVKQLVGAQPKRKILAELE
                     PALQG"
     gene            171..347
                     /locus_tag="PLE93_05650"
     CDS             171..347
                     /locus_tag="PLE93_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNH86567.1"
                     /translation="MPFVQIHWFEGRSDEQKAEIAKRIEEALIEVAGAAPEHCWVKFE
                     DSKKTDFIIPEARS"
     gene            505..876
                     /locus_tag="PLE93_05655"
     CDS             505..876
                     /locus_tag="PLE93_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH86568.1"
                     /translation="MGVLNTDLGRQLEAKELGARLWRIEPGQASTRHRHKTTEELYLL
                     LEGTGKLRIGEELLTLEPMDAVVVEPETVRQPFNDTDADQLWLIVGAPTEHADTTSMD
                     EELIAWMYPDGPKAMPPELDP"
     gene            904..2082
                     /locus_tag="PLE93_05660"
     CDS             904..2082
                     /locus_tag="PLE93_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH86569.1"
                     /translation="MERPESAAQVVGKRTIFEAEHDDYRESFRRFLEKEVVPEYPQWE
                     RDHIVPKSLFTKCAEYGFLSMEVPEEYGGPGVDDWRFNVVLAEESVWAGVGAGMGGPL
                     LHTDIVVPYIMSTANEEQKQRWLPQIASGEKVLAVAMTEPGTGSDLAAISTRGKRDGE
                     GWIVNGAKTFITNGINADQVVVAVRTSDDPHGGLSLFVIEEGMEGFERGKQIEKLGQH
                     ASDTSELFFNDCYVPQENMLGEEGSGFLQLVSRLVPERLTLSVSSMAGAEAALAMTID
                     YAKERKAFGRPIANFQHNRFTLAELRTEIEIGRCFIDRCIERYVDGTCTVEEAAMAKW
                     WTTELVSKVTDAGVQLHGGYGYTTEYRIGKAWVDARVGRIYAGTNEIMKELIGKTMGL
                     "
     gene            complement(2084..>2354)
                     /locus_tag="PLE93_05665"
     CDS             complement(2084..>2354)
                     /locus_tag="PLE93_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86570.1"
                     /translation="LELWIASRTDDEMREHLVRIERDVGVAIVQESARALGPIADRPD
                     FNDDMIYILATMRGLSMMRISHGVSDRTLNRRWSEIRERLAQVLT"
BASE COUNT          445 a          728 c          790 g          391 t
ORIGIN      
        1 gatgacaacc cgcagaccgc ccagcagtac ggggttctct cgatcccgac gatgatcctg
       61 ctcaaggacg gcgccctggt caagcagctg gtcggcgccc agcccaagcg caagattctc
      121 gccgagctcg agccggccct tcagggctag ccggacgggg acggatcacc gtgccgttcg
      181 tgcagatcca ctggttcgag ggccgctccg atgagcagaa ggccgagatc gccaagcgca
      241 tcgaagaggc gctgatcgag gtcgccgggg cagctcccga gcattgctgg gtcaagttcg
      301 aggactcgaa gaagaccgac ttcatcattc ccgaggcgcg ttcctgagtc tgggggtgga
      361 caccgtgggg gttcccttgg tgtgcacagt cccagtcggg aacccccacg gcgcccaccc
      421 ctccgaacct ggaggaagac cggtcccgtg agtgggtttc gtcggttgag ggtgggggag
      481 cagttctggc ggcgttctaa ccagatggga gttctgaaca ccgatctggg tcggcagctt
      541 gaggcgaagg aactcggggc caggctctgg cggatcgaac cgggccaggc ctcgacccgg
      601 caccggcaca agacgaccga ggagctttac cttttgctcg aaggcaccgg caagctgcgg
      661 atcggggagg agctcctgac actcgaaccg atggacgcgg ttgtagtcga gccggagacg
      721 gtgcgccagc cgttcaacga caccgatgcc gaccagctct ggctgatcgt cggcgctccg
      781 accgagcatg ccgacaccac atcgatggac gaggagctga tcgcctggat gtacccggac
      841 gggccgaagg cgatgccgcc cgagcttgac ccctgatctg cgcaagcgga ctaggcttcc
      901 ggcatggaga gaccggagag tgcagcgcag gtagtcggca agcggaccat tttcgaagcg
      961 gagcacgacg actatcgcga gagctttcgt cgtttcctcg agaaggaagt ggtgcccgag
     1021 tacccgcagt gggagcggga tcacatcgtt cccaagagcc tgttcaccaa gtgcgccgag
     1081 tacggcttcc tctcgatgga ggtgcccgag gagtacggcg gccccggcgt cgatgactgg
     1141 cgcttcaacg tggtgctggc cgaggaatcc gtctgggccg gggttggcgc gggcatgggt
     1201 ggccccctgc tccacaccga catcgtcgtg ccctacatca tgagcacggc caacgaggag
     1261 cagaagcagc gatggctgcc gcaaatcgct tccggcgaga aggtgctggc cgtcgcgatg
     1321 accgaacccg gcaccgggtc cgacctggcc gcgatctcca cccggggcaa gcgggacggc
     1381 gaaggctgga tcgtcaatgg tgccaagacc ttcatcacca acggcatcaa cgccgatcag
     1441 gtcgtggtcg cggtccggac ctccgatgac cctcacgggg gtctgtcgct tttcgtgatc
     1501 gaagagggca tggaggggtt cgaacgtggc aagcagatcg agaagctcgg ccagcacgcc
     1561 tcggacacgt ccgagctctt cttcaacgac tgctacgtgc cgcaggagaa catgctcggc
     1621 gaggaaggct ccggtttcct gcagctggtc tcccggctgg tgccggaacg gctgacactt
     1681 tcggtttcat cgatggccgg cgccgaggct gccctcgcca tgaccatcga ttacgcgaag
     1741 gagcgcaagg ctttcggccg gccgatcgcc aacttccagc acaaccgctt caccctggcc
     1801 gaactgcgga ccgagatcga gatcggccgc tgcttcatcg accggtgcat cgagcggtac
     1861 gtggacggga cctgcacggt cgaggaggcg gcgatggcca agtggtggac caccgaactg
     1921 gtcagcaagg tgacggacgc cggagtccag ctccacggtg gttacgggta cacgaccgag
     1981 taccgcatcg gcaaggcctg ggtggacgcc cgggtcggcc ggatctacgc cggaaccaac
     2041 gagatcatga aggaactgat cggcaagacg atgggcctct gactcaggtc agaacctgag
     2101 ccagccgttc ccggatctcg ctccagcgtc ggttcagggt ccggtcgctt acaccgtggc
     2161 tgatcctcat catggacagg ccgcgcatgg tggcgaggat gtagatcatg tcgtcgttga
     2221 agtcgggccg gtcggcgatc ggaccgaggg ccctcgcgga ctcctgaacg attgccaccc
     2281 cgacgtccct ttcgatcctg accaggtgct cgcgcatttc atcgtcggtc cgtgaagcga
     2341 tccacagttc aagc
//
LOCUS       DAOQFI010000279         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25498_length_2348_cov_2.503707, whole genome shotgun sequence.
ACCESSION   DAOQFI010000279 DAOQFI010000000
VERSION     DAOQFI010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25498_length_2348_cov_2.503707"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1379
                     /locus_tag="PLE93_05670"
     CDS             93..1379
                     /locus_tag="PLE93_05670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNH86571.1"
                     /translation="MAEIVMPRLTDSMEEGVILAWLKADGDEISVGDELVEIETDKAS
                     MVYESDLAGTLEILVPEGETRPIGEPIANVGDGSSSRGGGAVAVGDPVGRTSPGVPPG
                     PPTATASSSEEGSPDLRETSEDSPSMPSVSDGESNSGERIKASPLARRIAEDLGVDLT
                     GLSGSGPGGRIIRVDVEGAASGASEETARKPDQAPVDSAKGTPEIVELNRTQQLIARR
                     MSESKATIPHFYLRAVVDMDRAVEVRAAFKEEAADNETVPSLNDFVVKAAAIALKRHP
                     LANAAFRDGHFELYPKVNVGIAVATEGSLIVPVIKDADHLTLTEISDETRRLAGRVRS
                     GEVTPPELAGGTFTVSNLGMFGVSGFDAVINPGQAGILSVGEVTERPVVRDGKIASAR
                     LMEITLACDHRILYGAEGAEFLASVKTNLEEPGLLA"
     gene            1435..2154
                     /locus_tag="PLE93_05675"
     CDS             1435..2154
                     /locus_tag="PLE93_05675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNH86572.1"
                     /translation="MSEEVQAPTSPAGNKGESEFVEYSRARQSMSRRIAESKATIPHI
                     YLQVEADVTALVRRRAALRAEGETATVNDLVIKATALALRNHSRVNSTYRDGGLELHS
                     RINIGVAVDTPDGSLTPVVTDADSKDLNEIATEVRDLSARARSGEITPPEQAGATFAI
                     SNLGTLGVTSSEPVVNPGQAAKLAVGAISSRPVVRGGEVVPGEVMTLDLSCDHRVLHG
                     GEAARFLNEVKANLENPGLLG"
     gene            2151..>2348
                     /locus_tag="PLE93_05680"
     CDS             2151..>2348
                     /locus_tag="PLE93_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86573.1"
                     /translation="MSAGATPDATGDGPRCVTNSAGVAAGRRVASSAGVAGSVAVTHS
                     AGVAGSAATGFSAGVAGSLATL"
BASE COUNT          414 a          776 c          804 g          354 t
ORIGIN      
        1 ccccatgagg aaaacgtgat cgaggcggtt ctggcgacga ccgaaggggc atttccgccc
       61 gccggcggta actccgggga aggtgattcc tgatggccga gatcgtcatg ccccgcctga
      121 ccgactcgat ggaagagggc gtgatcctcg cctggctgaa ggctgacggg gacgaaatct
      181 cggtcggtga cgagctggtc gagatcgaga ccgacaaggc ctcgatggtc tacgaatccg
      241 acctcgccgg aacccttgag atcctcgtcc ccgaaggcga aacccgcccg atcggggaac
      301 cgatcgcgaa tgttggtgac ggatcttcaa gccgtggtgg tggcgcggtg gccgttgggg
      361 atcccgtcgg ccggacttcg ccgggagttc cgccgggacc cccaacggcc accgcttcca
      421 gctccgaaga gggttcgcca gacctgcgcg agacctcaga agattcccct tcaatgcctt
      481 ctgtttccga tggtgagtcc aactcgggtg aacggatcaa agcttcgcct ctggcaagac
      541 gtatcgcgga ggatttggga gtagatctga ccggtctttc ggggtcgggt cccgggggtc
      601 ggatcatcag ggttgatgtg gaaggcgcgg cgagcggggc gtcggaggag accgcgagga
      661 aaccggacca ggccccggtt gactcggcca aggggacgcc cgagatcgtc gagctgaacc
      721 ggacccagca gttgatcgcg cggcgcatgt cggagtcgaa ggcgaccatc cctcacttct
      781 atctccgcgc cgtggtcgac atggaccgcg cggtcgaggt cagggccgcc ttcaaggaag
      841 aagccgccga caacgagacc gtcccctcgc tgaatgactt cgtggtcaag gctgcggcca
      901 tcgccctgaa gcggcatccg ctggccaacg ccgccttccg ggacggccac ttcgagctct
      961 accccaaggt caacgtcggg atcgcagttg cgaccgaggg ttcgctgatc gtgccggtga
     1021 taaaggacgc cgaccacctg accctgaccg agatcagcga tgaaacgcgg cgccttgccg
     1081 ggcgggtccg ctccggcgag gtcacgcctc ccgagctggc cggcggcacc ttcaccgtct
     1141 cgaacctcgg catgttcggg gtgagcggat tcgacgcggt gatcaacccc gggcaggcgg
     1201 gcatcctctc ggtgggagag gtcaccgagc gtccggtcgt gcgggacgga aagatcgcct
     1261 ccgcccgcct gatggagatc acgctcgcct gtgatcaccg catcctctac ggggccgagg
     1321 gagccgaatt ccttgcctcg gtcaagacga acctcgagga acccggcctg ctcgcctgaa
     1381 gacgggcgcc gcggggccga caccggagtc cggatgccca tatcgtcact ccagatgagc
     1441 gaagaggtac aggcacccac cagtccggcc ggcaacaagg gcgaaagcga gttcgtcgag
     1501 tactcacggg caagacagtc gatgtcgcgg cggatcgccg agtcgaaggc gaccatcccc
     1561 cacatctacc tgcaggtcga ggccgacgtc accgcactgg tccgccgccg ggcggccctc
     1621 cgggccgaag gcgagacggc gaccgtgaac gacctggtga tcaaggcgac ggcgctggcc
     1681 ctccgcaacc acagccgggt caacagcacc taccgtgacg gtgggctcga acttcattcg
     1741 cgaatcaaca tcggggtcgc tgtcgacacc ccggacggct cgctgacccc ggtcgtcacc
     1801 gacgccgaca gcaaggatct gaacgaaatc gcgaccgagg tgcgggacct ttccgcccgg
     1861 gctcgctccg gcgagatcac tccgcccgag caggccggcg ccactttcgc gatctccaac
     1921 ctcggcacgc ttggcgtgac cagctccgag ccggtggtca atcccggcca ggcggcgaag
     1981 ctcgcggtcg gggcgatcag ttcgcgtccg gtcgtccgcg gcggcgaggt cgtgcccggc
     2041 gaggtaatga ccctcgatct gtcctgcgat caccgcgtgc ttcacggggg cgaggcagcc
     2101 cgcttcctga acgaggtcaa ggccaacctg gagaacccgg ggctgctggg atgagcgccg
     2161 gggccacacc ggatgcgacc ggcgatggcc cccgctgcgt gaccaacagt gccggcgtcg
     2221 cggccggacg ccgggtagcg agctcggccg gagtcgcagg cagtgtcgcg gtgacccact
     2281 cggccggtgt ggccggcagt gccgcgaccg gattcagcgc cggcgtcgcg ggcagcctgg
     2341 cgaccctg
//
LOCUS       DAOQFI010000280         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25610_length_2342_cov_4.153476, whole genome shotgun sequence.
ACCESSION   DAOQFI010000280 DAOQFI010000000
VERSION     DAOQFI010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25610_length_2342_cov_4.153476"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2342)
                     /locus_tag="PLE93_05685"
     CDS             complement(<1..>2342)
                     /locus_tag="PLE93_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HNH86574.1"
                     /translation="MPNAVYTHVQWDTGDLAPGQLITYRYRAAVPLTENTLNWSGATP
                     ATTGAQGANLDNNAIAPPKEIQDEQKIVNYAIAPPKEIQDEQKIVNYAIASGDYQSDV
                     VTPFSVFDDTTLQRTAEDLVVYKSNLTSSTLAQGDVNTWELRFRTGEYRYSDDIVVTD
                     TLPSGYCPLGPVNYTTGNSPSDSECDPTGDNPSVPNKSVTENADGTFTIVWDKSVMAK
                     LGHTDVSDEFVITFPTKTRKNYQQNFQDTTPILAADSTSNKVDLTANAFSRCTSPGTP
                     DCATPGPRIWNDGQEPEPVVDASSAGQTAPQVTLKKEVAESGTNCQTATWVTNIPTYH
                     PGDTICWRLSINFPSKVDTHQLRVTDFLPRNAAYVPGSYQDTSGNTTINTIDTADSSD
                     GVLFWDINGSYVPVGSQKFQVTFKTTIEPTGILSPVDIEGNLMKFAFENTPGTSFPLR
                     DQVDFKTVAPTLKLTKGVSKIDSTNYSPPADGLTVRGGNDVTYQVDVKSTGDTKDVQV
                     WDKLPAAYTCSMVSLISDGGACVADSGVSRIKWPAIAAINDGNTKSLTYHVLIPGTFG
                     PENTYVNTAGVRQYESDTNTGGRYIYTPQNNIDPSNPRTPNIARVDDDSNVKTPDVGL
                     VKARTTSITETGNSATSQATIGELINYTVTATLPNGTTFGTNAKITDTPNSATTQPLT
                     GPATATLNGSPLPSGWSISTVGQTITVNIPDGYVVPPGSDHTVVISFQTRVVDVASNV
                     RGQSRTNQANISWTDSTARSRNSNQVSTTIVEPLISQSKS"
BASE COUNT          344 a          625 c          859 g          514 t
ORIGIN      
        1 cgacttgctc tgcgagatca gcggctcgac gatggtggtc gagacctggt tcgagttgcg
       61 gctgcgggcg gtgctgtcag tccaggagat gttggcctga ttggtccggc tctgaccgcg
      121 gacatttgac gccacatcga cgacccgggt ctggaaactg atcacgaccg tgtggtcgga
      181 tcccggcgga accacgtatc cgtcggggat gttgaccgtg atggtctggc cgaccgtgct
      241 gatgctccag ccggagggca gcggtgaacc gttcagggtc gcggtcgccg ggccggtcag
      301 cggctgggtc gtggccgagt tcggggtatc ggtgatcttc gcgttcgtac cgaaggtagt
      361 gccattcggc agggtcgcgg tgaccgtgta gttgatcagc tcgccaatcg tcgcctggga
      421 agtggcgctg ttgccggtct cggtgatcga ggttgtccgg gccttgacca acccgacatc
      481 gggggtcttg acgttgctgt cgtcatcgac ccgggcgatg ttcggggtgc gcggattgct
      541 cggatcaatg ttgttctgcg gcgtgtagat gtagcgaccg ccggtattgg tgtcgctctc
      601 gtactgacgc acgcccgccg tgttgacgta agtgttctcc ggcccgaagg ttccggggat
      661 gaggacgtgg taagtcagtg acttggtgtt gccgtcattg atcgcggcga tggcgggcca
      721 cttgatgcgg ctgacgccgg agtcggcgac gcaggcgccg ccgtcggaga tcaaggagac
      781 catggagcag gtgtaagcgg ccgggagctt gtcccagacc tggacgtcct tggtatcgcc
      841 ggtcgacttg acatctacct gataggtgac gtcgtttcct ccgcggactg tcaggccgtc
      901 ggcgggagga ctgtagttcg tcgagtcgat cttgctgaca cccttggtca gcttcagggt
      961 cggagcgact gtcttgaagt cgacctggtc acgcagcggg aacgaggtgc ccggcgtgtt
     1021 ttcgaaggcg aacttcatca ggttgccctc gatgtcgaca gggctgagaa tgccggtcgg
     1081 ctcgatcgtc gtcttgaagg tgacctggaa cttctgcgat ccgaccggca cgtagctgcc
     1141 gttgatgtcc cagaagagca cgccgtcact ggaatcggcc gtgtcgatcg tgttaatcgt
     1201 ggtgttgcca gaggtgtcct ggtagctgcc cgggacgtaa gcggcgttcc gcggcaggaa
     1261 gtcggtgact cgcagttgat gggtgtcgac cttggacggg aagttgatac tgaggcgcca
     1321 gcagatcgtg tcgcccgggt ggtaggtcgg gatgttcgtg acccaggtgg ccgtctggca
     1381 gttggtgccg gactcggcga cttccttctt cagggtgacc tgaggggcgg tctggccagc
     1441 cgaggaagcg tcgaccaccg gctcgggctc ctgcccgtca ttccagatcc gggggccggg
     1501 ggtggcgcag tccggggtgc cgggcgaggt gcagcgcgag aacgcgttgg cggtcaggtc
     1561 gaccttgttg gaggtcgagt cggcggccag aatcggcgtc gtgtcctgga aattctgctg
     1621 gtagttcttg cgagtcttcg tcgggaaggt gatgacgaat tcgtcgctca cgtcggtgtg
     1681 gccgagcttc gccatgaccg acttgtccca gacgatcgtg aaggtgccgt cggcgttctc
     1741 ggtcacgctc ttgttcggca cgctcgggtt gtcgccggtc gggtcgcatt cggaatcgga
     1801 agggctgttg ccggtcgtgt agttgaccgg gccgagcggg cagtaaccgg aaggcagtgt
     1861 gtcggtgacg acgatgtcat cgctgtagcg gtactcgccg gtgcggaagc ggagctccca
     1921 ggtgttgaca tcaccctggg cgagcgtgct cgaggtcaga ttcgacttgt agacgacgag
     1981 atcctcggcg gtgcgctgaa gggtggtgtc atcgaagacg ctgaagggag taaccacatc
     2041 gctctggtag tcaccggagg cgatcgcgta gttgacgatc ttctgttcgt cctggatctc
     2101 cttcggcggg gcgatcgcgt agttgacgat cttctgttcg tcctggatct ccttcggcgg
     2161 ggcgatcgcg ttgttgtcca ggttcgcacc ctgggccccg gtggtcgccg gggtggcgcc
     2221 gctccagtta agggtgttct cggtcagcgg gaccgcggca cggtagcggt aggtgatcag
     2281 ctggccgggg gcgaggtcgc cggtgtccca ctggacatgc gtgtagaccg cgttcggcat
     2341 cg
//
LOCUS       DAOQFI010000281         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25615_length_2342_cov_3.183209, whole genome shotgun sequence.
ACCESSION   DAOQFI010000281 DAOQFI010000000
VERSION     DAOQFI010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25615_length_2342_cov_3.183209"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLE93_05690"
     CDS             complement(<1..241)
                     /locus_tag="PLE93_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86575.1"
                     /translation="MIPAARFGAAVLLLASLVAGCGSSTDQEGGGNAAAKSGQATTAR
                     ERAADRAAARKARAAARQKAALAARQARARARREAR"
     gene            complement(238..1854)
                     /locus_tag="PLE93_05695"
     CDS             complement(238..1854)
                     /locus_tag="PLE93_05695"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNH86576.1"
                     /translation="MTEGLTRRDALRAGAGAAGGMALAGGLLGRAIDAMGAPAVIGDG
                     PYGPLGTPDANGLRLPAGFTSRKIARSGENIGPRPYQFHILPDGMGSFKTSDGGFILV
                     SNSEAPYLPGLAEIGAGAIRFNKDFEVVDAYPILKETWVNCAGGVTPWGTWLSCEEIE
                     TGKVFECDPKGRKPFDGSNWIERPALGVFKHEACCVDPAGKQVYLTEDLGDGCLYRFT
                     PNRYPDLSSGRLDVASVANDGTVTWHAVPDPQYAGSVPTRHQVEAATHFRRGEGMWFD
                     AGKVYFSTTQDDRVYAYHPASAHLEVLYDGVALGDEAPLHDTDNVTVSPISGDLFVCE
                     DPGGNAVNGLQVCIISAEGEAAAFCEAPASGHKDSELTGPVFDPTGSRLYFSSQRAKF
                     PGETVSAGIIYEISGPFRKLREDEKPVDHKKPLVKVRTLGMPSLAGLVKNGQAFQIDV
                     HDQSFPVDVEARLIAPLRRRQGRGTREVTLGTLKLTLHRPGAKKIRLKIKEGYRKRIR
                     QRRISGARLELIARDAAGNQRKVIKQVSFR"
     gene            complement(1983..>2342)
                     /locus_tag="PLE93_05700"
     CDS             complement(1983..>2342)
                     /locus_tag="PLE93_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNH86577.1"
                     /translation="SQDIPLQFLEHILLDLKHSGIVRARRGAKGGYWLAKPAEDVSIA
                     DVIRAVEGPIAHVQSAPPESITYTGSARELQNVWIAVRASLRDVLESTSLADLVSGNL
                     PDNVQRLADDKDAWEPR"
BASE COUNT          377 a          753 c          795 g          417 t
ORIGIN      
        1 ggcgtgcttc ccgtctggcc cgtgccctcg cctgcctggc ggcaagcgcg gccttctgac
       61 gagcggccgc cctggctttg cgggctgccg cacgatcggc agcccgttcc cgggccgtgg
      121 tcgcttggcc ggatttggcg gcggcgtttc cgcccccttc ctggtccgtg gatgaaccac
      181 agcccgcgac aaggctggcg aggagcaaaa cggccgcccc gaagcgggcc gccgggatca
      241 acggaaactg acctgcttga tcacttttcg ctgattgcca gcggcgtctc tggcgatcag
      301 ctccagtctg gccccggaga tccgccgctg cctgatcctc ttgcggtagc cctccttgat
      361 cttcaggcga atctttttgg cgcccggacg gtgaagggtg agcttcaggg ttccgagcgt
      421 cacttcgcgt gtgccgcggc cctgccggcg gcgcaagggc gcgatgagcc gggcctcgac
      481 atcgaccggg aagctctggt cgtgaacatc gatctggaag gcctggccgt tcttgacgag
      541 cccggcgagg cttggcatcc ccagcgtccg caccttgacc agcggcttct tgtgatcgac
      601 cggcttttcg tcctcccgca gtttccggaa ggggccgctg atctcgtaga tgattccggc
      661 ggacacggtt tcaccgggga acttcgcccg ttgtgaactg aagtagagcc ggcttccggt
      721 cgggtcgaat accggcccgg tcaactccga atccttgtgg ccgctggccg gcgcctcgca
      781 gaaggcggcc gcctcgccct cggccgagat gatgcagacc tgcaggccgt tgacggcatt
      841 gccgcccggg tcctcgcaga cgaaaaggtc gccggagatc ggtgagacgg tcacgttgtc
      901 ggtgtcatgg aggggagctt cgtcgccgag ggcgaccccg tcatagagca cctcgagatg
      961 agccgaagcc gggtgatagg cataaacgcg gtcgtcctgg gtggtcgaga aatagacctt
     1021 cccggcgtca aaccacatcc cttcgccccg ccggaaatga gtcgctgcct cgacctgatg
     1081 acgtgtggga accgagccgg cgtactgggg gtccgggacc gcatgccagg tgacggttcc
     1141 gtcgttagcg accgaagcga cgtcgagccg gccggatgaa aggtccgggt accggttggg
     1201 agtgaagcgg taaaggcaac cgtccccgag atcctcggtc aggtaaacct gcttgccggc
     1261 cggatcgacg cagcaggcct catgcttgaa cacgccgagc gccgggcgtt cgatccagtt
     1321 gctgccgtcg aacggtttcc ggcctttggg gtcacattcg aaaaccttgc cggtctcgat
     1381 ctcctcgcag gaaagccagg ttccccacgg ggtcacgccg cccgcgcagt tgacccaggt
     1441 ttccttgagg atcgggtagg cgtcgaccac ttcgaaatcc ttgttgaagc ggatcgctcc
     1501 ggctccgatt tcggccaggc ccggcaggta gggggcctcg ctgttcgaaa cgaggatgaa
     1561 gccgccgtcg ctggtcttga aggagcccat gccgtcgggc aggatgtgaa actggtacgg
     1621 gcggggcccg atgttctcac cggagcgggc gatctttcgg gaagtgaagc cggcggggag
     1681 ccggaggccg ttggcatccg gggtcccgag cggaccgtag gggccgtcgc cgatcaccgc
     1741 cggagcgccc atcgcgtcga ttgcccggcc cagcaggccg ccggccagag ccattccccc
     1801 ggcggcgcca gccccggccc gcaaggcgtc tcttcgagtc agtccttcag tcacttcctc
     1861 tgacataccc actccctgga aatcgtagaa aaactctccc tgaaagcagg ctaccggtca
     1921 gggcaaggcc agggcaacac aagtcatggg cgggcggtgg tccctcgcgc cgcccggggc
     1981 acctacctgg gttcccaggc gtccttgtca tcggccagcc gctgaacgtt gtccgggagg
     2041 ttcccggaga cgaggtcggc gaggctggtt gattccagca cgtcacggag gctggcgcgg
     2101 acagcaatcc agacattctg gagttccctg gcgctgccgg tgtaggtgat cgactcgggc
     2161 ggagccgact ggacgtgggc gatcggacct tcgaccgcgc ggatcacgtc ggcgatcgaa
     2221 acgtcttcgg ccggcttggc cagccagtag ccacccttgg cgccgcgacg ggcgcggacg
     2281 atccccgagt gcttcaggtc gagcaggatg tgctcgagaa actgaagcgg gatgtcctgg
     2341 ga
//
LOCUS       DAOQFI010000282         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25809_length_2332_cov_3.143610, whole genome shotgun sequence.
ACCESSION   DAOQFI010000282 DAOQFI010000000
VERSION     DAOQFI010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25809_length_2332_cov_3.143610"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLE93_05705"
     CDS             complement(<1..262)
                     /locus_tag="PLE93_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNH86578.1"
                     /translation="MAPEDPVELSGNRLEVVRAFKNAYRTLSRRKGRETHRLGATEVS
                     QSQFELLVELHKSGPLAVGELAQTNGLSPASVSQMVDRLSDQG"
     gene            complement(252..1706)
                     /locus_tag="PLE93_05710"
     CDS             complement(252..1706)
                     /locus_tag="PLE93_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNH86579.1"
                     /translation="MNQDSTTRIEPYVWKVAGVVILGMIMSILDTTIVNVALDTLAKD
                     LHTTISEIQWVATGYLLSLAAVIPLTGWAARKFGAKNVYIWSIVLFTLGSALCGIAAS
                     STSLIAFRVIQGIGGGMIMPIAQMIMAEVAGPKRMGKVMSVTVMPAMLAPILGPTIGG
                     LILQNFHWSWIFFVNVPVGIVAALLAVRMLPHSKLGEPGKLDWAGLVLMVLGIPSLVF
                     GISEVGTTGTFNSPEVIWPVIAGLLLIGAFIRHAYRAERPLLDVRLYANRVFGAASLT
                     TFALGAALFGAMILMPLYYQQIRGESVIATGLLLAPQGLGMLMVGPFAPRLTERFGGG
                     KVATFGVAVLSLGSLPFVFIGVDTSIAFLSLMLLVRGIGIGLAFMPTMTVAYASLRKD
                     QLSDATPQMNVIQRVGGAIGTAVLAVVLQQASEGATTATAAAQGFGTAYLWALGIALL
                     ALIPCVVLWRAEMPAGPVTEEERIAEVEIEPLGA"
     gene            complement(1757..>2332)
                     /locus_tag="PLE93_05715"
     CDS             complement(1757..>2332)
                     /locus_tag="PLE93_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86580.1"
                     /translation="DGMSPRHYPLDKKRIAFGFAIEHLGTREVIPTGEGTSRHLAFTG
                     VADPALFGAGDSDALREAAVEATKRRNLPRTAVLKGLSVPNPDQAPSICSMGGLGNSF
                     QPELVPTLAMISGPWSLYDPVFGEKAIDFGQMRKQVLAAGDTVLALDGLPRAEIAGEY
                     PALQAELDAGTKTPCPETDVFPQRAPGPGGV"
BASE COUNT          421 a          763 c          766 g          382 t
ORIGIN      
        1 gcccctggtc ggaaaggcgg tcgaccatct gggaaacgct ggccgggctc aggccgttgg
       61 tctgggcaag ctcgccgacc gccagggggc ccgacttgtg aagttcgacc agaagctcga
      121 actgggactg gctcacctcg gtcgcgccga gccggtgggt ctcgcgcccc ttgcgccggc
      181 tcagggtgcg gtaggcgttc ttgaacgccc ggacgacttc cagccggttt ccgctgagtt
      241 cgactggatc ttcaggcgcc aagcggttca atttcaacct ccgcgattcg ttcttcctcg
      301 gtcaccggcc cggccggcat ctccgcccgc cacagcacca cgcaggggat cagggccagc
      361 agggcgatcc cgagcgccca aaggtaggcg gtcccgaacc cctgtgccgc cgccgttgcc
      421 gtggttgcac cctcgcttgc ctgctgcagg acgaccgcca gtaccgccgt gccgatcgct
      481 ccacccaccc gctggatcac gttcatctgc ggcgtggcat cggaaagctg gtccttcctc
      541 agcgatgcgt aggcgacggt catcgtcggc atgaaggcaa ggccgatgcc gatgccccgg
      601 accagcagca tcaacgagag gaaggcgatc gaagtgtcaa ccccgatgaa gacgaaggga
      661 agcgatccga ggctgagcac ggcgactccg aaggtcgcga ccttgccgcc gccgaagcgc
      721 tccgtgaggc ggggcgcgaa gggcccgacc atcagcatgc ccaggccctg cggggccagc
      781 aggaggccgg tcgcgatcac gctttcgcca cggatctgct ggtagtagag cggcatcagg
      841 atcatcgctc caaagagcgc ggcacccagc gcgaaggtgg ttagcgaggc ggcaccgaag
      901 acccggttcg catagagccg gacgtcaagc agcggccgct cggcgcggta tgcgtgccgg
      961 atgaaggcgc cgatcaggag cagtccggcg atgaccggcc agatcacttc cggcgagttg
     1021 aaagttccgg tggtgccgac ttcggagatg ccgaagacca gggacggaat gccgagcacc
     1081 atgaggacca gtccggccca gtcgagtttg ccgggctcgc cgagtttcga atggggcagc
     1141 atcctcaccg cgagcagcgc ggcgacgatg ccgaccggaa cgttgacgaa gaagatccag
     1201 gaccagtgaa agttctgcag gattagaccg ccgatcgtcg ggcccagaat cggggcgagc
     1261 atggcgggca tcacggtgac gctcatgacc ttgcccatcc ttttcggtcc ggcgacctcg
     1321 gccatgatca tctgggcgat cggcatgatc atccctccac cgatcccctg gatcacccgg
     1381 aaggcgatca gcgaagtgct cgatgcagcg atgccgcaaa gggccgagcc gagcgtgaac
     1441 aggacgatcg accagatgta gacgttcttt gccccgaatt tccgcgccgc ccagccggtc
     1501 agggggatca ccgccgcgag cgaaagcagg tagccggtcg ccacccactg gatctcgctg
     1561 atcgtcgtgt gcaggtcttt ggcgagggtg tcgagcgcga cgttgacgat cgtcgtgtcg
     1621 agaatcgaca tgatcatgcc gaggatcacc accccggcga ccttccagac gtacggctcg
     1681 atgcgggtag tggaatcctg attcattagg aacctaatat taggctccta atcactttcc
     1741 gtcaagtgtg aatccgtcac actcccccgg gccccggtgc ccgctgcggg aagacatcgg
     1801 tttccgggca gggcgtcttg gtgccggcat ccagctccgc ctgcagggcc ggatactcgc
     1861 cggcgatctc ggcgcgcggc agcccgtcga gagccaggac cgtgtccccc gccgccagca
     1921 cctgcttgcg catctggccg aaatcaatcg ccttctcgcc gaacaccggg tcgtacaggg
     1981 accagggtcc agagatcatc gccagggtcg gaaccagttc aggctggaag gagttgccga
     2041 gccctcccat cgagcagatc gacggggcct ggtcggggtt gggcacgctc agccccttga
     2101 gcaccgccgt gcggggaagg ttccttcgct tggtggcctc gaccgcggct tcccgcagcg
     2161 cgtcactgtc accggcgcca aagagtgccg ggtcggccac cccggtgaag gcgagatggc
     2221 ggctcgttcc ctctccggtc ggtatcacct cgcgggtacc gagatgctcg atcgcgaaac
     2281 cgaacgcgat ccgcttcttg tccagcgggt aatggcgggg gctcatcccg tc
//
LOCUS       DAOQFI010000283         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25835_length_2331_cov_2.843585, whole genome shotgun sequence.
ACCESSION   DAOQFI010000283 DAOQFI010000000
VERSION     DAOQFI010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25835_length_2331_cov_2.843585"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="PLE93_05720"
     CDS             <1..1171
                     /locus_tag="PLE93_05720"
                     /inference="COORDINATES: protein motif:HMM:NF037020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH86581.1"
                     /translation="IVMIVFGIGMAAQWDLKFQNWIADDLPAIVVNPTKDLESSDATS
                     SRLADLRAGPQSASAAKIAAGSAVVQQEARAASDLPVIGKAPEFQGTQEWFNTPGDKP
                     LTMAGLRGQVVLIDFWTYTCINCIRTQPWLNAMEERYGDKGLTIVGVHTPEFPFEKDA
                     GNVEDAIGRAGIKYPVVQDNDYTVWNSYGNQYWPAEYFVDADGNVRYAHFGEGDYSHK
                     EEVIRELLAEAGHDPGAGQTGATGMKAAKVLLTPESYLGSARADRFENGTILPGVHDF
                     GPLKEPKLDWLAYGGKWSISPEDARSDGGQIAVHFRARRVYLVLSSPGKARSAGIELD
                     GKPVSAAAAGDDVKSGQVEVTSQRLYSLIDLPKPGEHVLTVSPEAGISGYAFTFG"
     gene            1237..1310
                     /locus_tag="PLE93_05725"
     tRNA            1237..1310
                     /locus_tag="PLE93_05725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1271..1273,aa:Arg,seq:acg)
     gene            complement(1465..1779)
                     /locus_tag="PLE93_05730"
     CDS             complement(1465..1779)
                     /locus_tag="PLE93_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HNH86582.1"
                     /translation="MTQTKAERSAAAKKAAATRKRNEAKAEAAASDRGKKAAATRQSK
                     AAKQSADQAKRSAGQAKQTAAEAKDSTGSAVGSLANAAKSAGDAALKAGKSVASRVRS
                     GF"
     gene            complement(1833..>2331)
                     /locus_tag="PLE93_05735"
     CDS             complement(1833..>2331)
                     /locus_tag="PLE93_05735"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86583.1"
                     /translation="TTSGSGSGTVTSSPAGISCGSGCSAHYDDGTPVILVAAPAAGST
                     FTGWSGTCSGTGVLCTVTMTEARSVSAAFASIPPGTTITRTPKKKIRTKAKRLTVRFA
                     FRSSQAGSTFECRIDRRPFTSCRSPLRLKVKRGRHEFQVRARNAAGVADPTPASYRFR
                     ILKKR"
BASE COUNT          385 a          758 c          797 g          391 t
ORIGIN      
        1 gatcgtgatg atcgtcttcg ggatcggcat ggccgcccag tgggacctca agttccagaa
       61 ttggatcgcc gacgacctgc cggccattgt ggtcaacccg accaaggacc tcgagtcgag
      121 tgatgccact tcgagccggc tggccgacct gagggccggc ccccagtcgg cctccgccgc
      181 gaagatcgcc gccgggtcgg cggtggtgca gcaggaggcc cgggccgctt cggatctccc
      241 ggtgattggc aaggcgccgg agttccaggg cacccaggaa tggttcaaca cgccgggcga
      301 caagccgctg accatggccg ggctgcgcgg tcaggtagtg ctgatcgact tctggaccta
      361 cacctgcatc aactgcatcc gcacccagcc ctggctcaac gcgatggagg agcgttacgg
      421 ggacaagggc ctgaccatcg tcggggtcca cacccccgag ttccctttcg aaaaggacgc
      481 cggcaacgtc gaggatgcga tcgggcgggc aggcatcaag tacccggtgg tccaggacaa
      541 cgactacacg gtctggaact cctacggcaa ccagtactgg ccggccgagt atttcgtcga
      601 cgccgacggc aacgtccgct acgcccactt cggcgagggc gactacagcc acaaggaaga
      661 agtgatccgc gaactactgg ccgaggccgg tcacgatccc ggcgcagggc agacgggcgc
      721 gaccggcatg aaggcggcca aggtcctcct gaccccggag agctatctcg gttcggcccg
      781 ggccgaccgc ttcgagaacg gaacgatcct gcccggcgtt cacgacttcg ggcccctgaa
      841 ggagccgaag ctcgactggc tcgcctacgg cggcaagtgg agcatcagcc cggaggacgc
      901 acggtccgac ggcggccaga tcgcggtcca cttccgggcc aggcgggtct atctggtcct
      961 ttcctcaccg ggcaaggccc gcagcgccgg gatcgaactg gacggcaagc cggtcagtgc
     1021 cgcggcggcc ggagatgacg tgaaaagcgg tcaggtcgag gtcacgagcc agaggctcta
     1081 cagcctgatc gacctgccga aaccgggcga gcacgtgctg acggtcagcc cggaagcggg
     1141 catcagcggc tacgccttca cgttcggctg agccagcagg agtcccgaac gctcgcaacc
     1201 tcgtagaatc gccgatccgg ggtgtttccc caatccgcgc ccgtagctca gctggataga
     1261 gcgttggtct acgaaaccaa aggtcacagg ttcgaatcct gtcgggcgcg cttgctggcg
     1321 gggcagcctt cgcgctacca gccgcctgtt cccgggtcgc ctttgatcgg gccggtgaag
     1381 ccgcaggttc gtgaacaggt tcggaccctc gttcacctgg gctttgacgc cccaggcgtt
     1441 ggcgagggcc cgggccccgg ggatctagaa gccgctgcgg acgcgggaag cgaccgactt
     1501 gccggccttg agggcggcgt caccggccga cttggccgcg ttggcgaggc tgccgacggc
     1561 cgatccggtc gaatctttgg cctcggcggc tgtctgcttc gcctgcccgg ccgaccgctt
     1621 ggcctgatcg gcggactgct tggctgcctt gctctgacgg gtagcggcgg ccttcttgcc
     1681 acggtcgctg gcggccgctt cggccttggc ttcgtttcgc ttccttgtgg cagctgcctt
     1741 ctttgcggcg gcgctgcgtt cagctttggt ctgagtcatg acactccggt acccggacca
     1801 aaaaccttca atcgtgagga atcgactccg ggttaccgct tcttcaaaat cctgaagcgg
     1861 taactggccg gggtcgggtc ggctactccg gctgcgttgc gggcgcgaac ctggaactcg
     1921 tgcctgcctc gcttgacctt gagccggagc ggggagcggc aggaagtgaa cggccggcgg
     1981 tcgatcctgc attcgaaggt cgagccggcc tggctggacc tgaaggcgaa cctcaccgtc
     2041 aatcgcttcg ccttcgtccg gatcttcttc ttcggggttc tcgtgatcgt ggtcccgggc
     2101 gggatcgagg cgaaagcggc gctgacggaa cgagcctcgg tcatcgtcac ggtgcagaga
     2161 acgccggtac cggagcaggt tccgctccag cccgtgaagg tcgaaccggc ggcgggggcg
     2221 gcgaccagga tcacgggcgt accgtcgtcg tagtgcgcgc tgcagccgga tccgcacgag
     2281 atcccggccg gcgacgaggt gacggtgcca ctcccggagc cggacgtggt c
//
LOCUS       DAOQFI010000284         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25858_length_2330_cov_3.209670, whole genome shotgun sequence.
ACCESSION   DAOQFI010000284 DAOQFI010000000
VERSION     DAOQFI010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25858_length_2330_cov_3.209670"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2299)
                     /locus_tag="PLE93_05740"
     CDS             complement(<1..2299)
                     /locus_tag="PLE93_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH86584.1"
                     /translation="MFRQNRDQGLPEARAAAKDQKSDITGAQSEVIDDLPANADPIYR
                     THSVLAAVGVTAPAGDKTLLEQIPGVSAVYPVAPKSPTNDYAVPFQAAAQAWQSTGFL
                     GQGQTIAVIDTGVDYTHSNFGGPGTETAFETAQATEDQPANPALFPNAKVSGGIDLVG
                     DDYDSDSDSENYQPDPHPDPNPLDCGGHGSHVAGSAAGYGVKADGSTYDGVYDNSTDF
                     AAMKIGPGMAPRAKVYAIKVFGCDGSTDVVTEAIDRAVDPNNDGDPTDHVDVINMSLG
                     SDFGSVQDGDSVASNAAVQMGVSVVASAGNSGDISDITGSPGDASQVLSVASSVDAES
                     KVDGAEVTIDGNQDLYAITRSGRYDWKTGPDLSGPVVAAPVENETACAPYTGTPFTGK
                     VVLVKWHDAVPECGSITRGDNLAAAGAIGFIFGSDAETFSAGINGDDAIPGVLMAKSG
                     ADAIRDALDNSLAVSVDGTEVNAVTRSFPDDNDKISSFTSRGIHATGNVKPDVTAVGS
                     SVFSTAVGTGDDGVSFSGTSMASPMVAGLAALVRQANPGWTPLQVKADIMNTAAHDLF
                     VDGSANPSSDRYGPPRVGSGRIDAALATGNRVLAYDPANGAVSVSFGPVAASAPVTLS
                     REVKVENQSSFPVTYDLAYDPINEVPGAEFSVTPSQVSLDPGRSTMVSVSLAIEDPSQ
                     LTKAVDPTAGRFSSEGDPRETLAEAFGRLLLEPTGPGTTLRVPVYASPRPASDMGQPA
                     SLLIHRSSATAGDPEQTASLELAGQG"
BASE COUNT          351 a          750 c          828 g          401 t
ORIGIN      
        1 ggccctggcc ggccagctcg aggctcgcgg tctgctcggg atcgccggcg gtggcggagc
       61 tgcgatgaat cagaagcgaa gcgggctggc ccatgtccga ggccggccgg ggagatgcgt
      121 aaaccgggac ccgtagggtc gtgccggggc cggtcggctc gaggagcagc cgcccgaacg
      181 cctcggccag ggtctcgcgc gggtcaccct cggagctgaa gcggccggcg gtcggatcga
      241 ccgccttggt cagctgggag gggtcctcga tcgcgaggct gaccgagacc atggtcgacc
      301 tgcccggatc caggctgacc tgggaaggcg taaccgagaa ttcggcgccc ggaacctcgt
      361 tgatcgggtc ataggcgagg tcgtaggtca ccgggaagga agactggttc tccaccttga
      421 cctcacgact gagggtcacc ggtgcgctgg cggcgaccgg gccgaaggac acactgaccg
      481 ctccgttggc cgggtcgtag gccagcaccc tgttgccggt ggcgagtgcc gcgtcgatcc
      541 ggccggaacc gacccggggc gggccgtagc gatccgagga cggattggcc gaaccgtcga
      601 cgaagaggtc gtgcgcagcc gtgttcatga tgtccgcctt gacctggagc ggagtccagc
      661 ccggattggc ctgccggacc agggccgcca gcccggcgac catcggcgaa gccatcgagg
      721 tgccggagaa gctgaccccg tcgtcacctg tgccgaccgc ggtcgagaag accgagctgc
      781 cgaccgcggt cacgtccggc ttgacgttgc cggtcgcgtg aattccacgc gaggtgaagc
      841 tcgagatctt gtcgttgtca tcagggaagc tccgggttac cgcgttgacc tcggttccat
      901 cgacgctcac cgccagcgag ttgtcgagcg cgtcgcggat cgcgtccgcc cccgatttcg
      961 ccatcaacac gccggggatc gcgtcgtcac cgttgattcc ggcggagaag gtttccgcgt
     1021 cgctgccgaa gatgaagccg atcgctccgg ccgcggcgag gttgtcgcca cgggtgatcg
     1081 aaccgcattc cggcaccgcg tcatgccatt tgacgagcac caccttgccg gtgaacgggg
     1141 tcccggtgta cggggcgcag gcggtttcat tttccaccgg agcggccacc accggcccgc
     1201 tgaggtcggg acccgtcttc cagtcgtaac ggcccgacct ggtgatcgcg tacagatcct
     1261 ggttcccgtc gatcgtgacc tcggcgccat ccaccttcga ctcggcgtca acgcttgagg
     1321 ccacgctcag aacctgcgag gcatctccgg gcgagccggt gatgtcagag atgtcaccgg
     1381 agtttccggc cgaggcaacc acgcttaccc ccatctgaac cgcggcgttg gaggcgaccg
     1441 aatccccgtc ctgaaccgat ccgaaatcgg agccgagcga catgttgatc acgtcgacat
     1501 ggtcggtcgg gtcgccgtcg ttgttcgggt cgaccgcgcg gtcgatcgcc tcggtcacca
     1561 cgtcggtgct gccgtcgcag ccgaagacct tgatcgcgta gaccttcgcc ctgggcgcca
     1621 tgcccggacc gatcttcatc gccgcgaagt cggtggagtt gtcgtagacg ccgtcatagg
     1681 tcgagccgtc ggccttgacg ccgtatccgg cggccgagcc ggccacgtgg gagccatgac
     1741 cgccgcagtc cagcgggttc ggatcggggt gagggtcggg ctggtagttc tcggaatcgg
     1801 aatcggagtc atagtcgtct ccgaccaggt cgatcccgcc ggagaccttg gcgttgggaa
     1861 acagggccgg gttggcgggc tggtcctcgg tcgcctgggc ggtctcgaag gcagtctcgg
     1921 tgccgggacc gccgaagttc gagtgggtgt agtcgacgcc ggtatcgatc acggcgatcg
     1981 tctggccctg gccgaggaag ccggtcgact gccaggcctg ggcggccgcc tggaacggca
     2041 ccgcgtagtc attggtcggg ctcttgggcg ccaccgggta gaccgcgctg acgccgggga
     2101 tctgttcgag cagggtcttg tcaccggccg gggcggttac gccgaccgca gccagcacgc
     2161 tgtgggtccg gtagatcggg tcggcgttcg cggggaggtc atcgatcacc tcgctctggg
     2221 ctccggtgat gtccgacttc tgatccttcg ccgcggccct tgcctcgggc aggccctgat
     2281 cgcggttctg ccggaacacg cggacggtcg aaggttcgtc gagtttcagc
//
LOCUS       DAOQFI010000285         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_25970_length_2324_cov_3.192155, whole genome shotgun sequence.
ACCESSION   DAOQFI010000285 DAOQFI010000000
VERSION     DAOQFI010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25970_length_2324_cov_3.192155"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLE93_05745"
     CDS             <1..289
                     /locus_tag="PLE93_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86585.1"
                     /translation="EQDGFSLRLRISLPAELSRYVIPQGSITIEGVSLTVADCGEDWA
                     EVALIPETRERTNLGTLQIGTTVNIECDVIAKYVERMVSPYAPGSESHTDR"
     gene            345..1652
                     /locus_tag="PLE93_05750"
     CDS             345..1652
                     /locus_tag="PLE93_05750"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNH86586.1"
                     /translation="MSTTEATPFATIEEALEEIRAGRMIIVTDDEDRENEGDLVMAAQ
                     FATPEAINFMAKEGRGLICLSLTAQRCDQLGLRLMTAKNEAPLETAFTVSIEAAEGVT
                     TGISAHDRAHTVQVAIDPDATPRDIVVPGHVFPLKAKDGGVLERTGHTEAAVDLARLA
                     GVSPAGVICEVMNEDGTMARVDDLVGYAEKHGLKMVTIADLIAYRRENEKLVERVVST
                     ALPTSFGDFRAVGFRSLVDDKHHVAMVKGEIDTGEPALVRVHSECLTGDVFHSMRCDC
                     GEQLAAAMAMIEDEGRGVLLYLSQEGRGIGLLNKLRAYKLQEEGLDTVDANLKLGLPA
                     DLRDYGIGAQILRDLGLTKIKILTNNPKKIIGLEGHGLEVTEQIKIEAPPNTHNESYL
                     RTKRDKMGHILHHQGLALDEEMILDEQIHDSEKATEAEGGRGA"
     gene            1645..2100
                     /gene="ribH"
                     /locus_tag="PLE93_05755"
     CDS             1645..2100
                     /gene="ribH"
                     /locus_tag="PLE93_05755"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNH86587.1"
                     /translation="MPEPEFTGRFCVVVGNFYTDLADRLVGGAKEEFARDGISDAEID
                     VYEVPGAFELPLAAGYAARSGRYDGVACLGAVIRGETDHYDFVCNEAARGIQNVQLET
                     GVPCGFGVLTVDNMEQALARAGGGGKRDSGANAARAVLRMNEIRRELSA"
     gene            2173..>2324
                     /gene="rpmB"
                     /locus_tag="PLE93_05760"
     CDS             2173..>2324
                     /gene="rpmB"
                     /locus_tag="PLE93_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNH86588.1"
                     /translation="MARVCHNCGKGPGFGNSRSHSMVATRRRFDPNLQKVRILEGGAK
                     KRVLVCT"
BASE COUNT          467 a          760 c          746 g          351 t
ORIGIN      
        1 cgagcaggac ggcttctctc tgcgactccg gatttcactg ccggcggaac tttcgcgtta
       61 cgtgatcccg cagggttcga tcacgatcga gggcgtcagc ctcaccgtcg ccgattgcgg
      121 cgaggactgg gccgaggtgg cgctgatccc cgaaacccgg gagcgcacca acctcggaac
      181 gcttcagatc ggcacaaccg tcaacatcga gtgtgacgta atcgccaaat acgtcgagcg
      241 catggtgtca ccatatgcac cagggtccga aagccacacg gaccgttgag aaaccaaaag
      301 gaaagtctga acaaagcatg gctaccgaag aagggcgcgg cagcgtgagt accactgaag
      361 cgactccgtt cgccacgatc gaagaggccc tggaggagat cagggccggc cggatgatca
      421 tcgtcaccga tgacgaggac cgcgaaaacg aaggcgacct cgtgatggcc gcccagttcg
      481 cgactcccga agcgatcaac ttcatggcca aggaagggcg ggggctgatc tgcctttcac
      541 tcaccgccca gcgctgcgac cagctcggcc tgcgcctgat gaccgccaag aacgaggcgc
      601 cgctggaaac cgccttcacc gtttcgatcg aagcggccga aggcgtgaca accggcatct
      661 ccgcccacga ccgggcccac acggtccagg tcgcgatcga ccccgacgcg actccgcggg
      721 acatcgtcgt ccccggacat gtattcccgc tcaaagccaa ggacggtggc gtgctggagc
      781 gaaccggcca caccgaggcc gcggtcgatc tcgcccgcct ggccggtgtt tccccggccg
      841 gcgtgatctg cgaggtgatg aacgaggacg gcaccatggc ccgggtcgac gacctggtcg
      901 gctacgccga aaagcacggc ctcaagatgg tcacgatcgc cgacctgatc gcctaccggc
      961 gcgagaacga gaaactggtg gaacgggtcg tctcgaccgc gctgccgacc agtttcggtg
     1021 acttccgcgc agtcggtttc cgctccctgg tcgacgacaa gcatcacgtc gcaatggtca
     1081 agggtgagat cgacaccggc gagcccgccc tggtccgggt gcattcggaa tgcctgaccg
     1141 gcgacgtctt ccacagcatg cgctgtgact gcggcgagca gctcgcggcg gcgatggcga
     1201 tgatcgagga cgaaggccgc ggcgtgctgc tctacctctc gcaggaaggc cgcgggatcg
     1261 gccttttgaa caagctgagg gcctacaagc tccaggagga agggctcgac acggttgatg
     1321 ccaacctcaa gctcggtttg ccggccgacc tgcgggacta cggcatcggt gcccagatcc
     1381 tgcgggatct cggcctgacc aagatcaaga tcctgaccaa caacccgaag aagatcatcg
     1441 gcctcgaagg ccacgggctc gaggtcaccg agcagatcaa gatcgaggcc ccgccgaaca
     1501 cccacaacga gagttacctg cggaccaagc gggacaagat gggtcacatc ctccaccacc
     1561 agggtcttgc cctcgacgag gaaatgatcc tcgacgagca gatccacgac agcgagaagg
     1621 cgaccgaagc cgaaggcggc cggggtgcct gaacccgaat tcacaggacg gttctgtgtg
     1681 gtggtcggca atttctacac ggacctcgcc gaccgtctgg tcggtggcgc gaaggaagaa
     1741 ttcgcgaggg acgggatctc cgacgccgag atcgacgtct atgaggttcc cggcgctttc
     1801 gaactgcccc ttgccgcggg ctacgcggcc cgttccggcc gctacgacgg agtcgcctgc
     1861 ctcggcgcgg tgatccgtgg cgagaccgac cactacgact tcgtctgcaa cgaggcggcc
     1921 cgcgggatcc agaacgtcca gctcgaaacc ggcgtcccat gcggtttcgg ggtgctcacc
     1981 gtcgacaaca tggaacaggc cctggcccgg gccgggggcg gcggaaagcg cgattccggc
     2041 gccaacgcgg cccgcgccgt gctgcgcatg aacgagatcc gcagggagct ttcggcctga
     2101 ccaagccgga ttccggccgg cggtgggcca cgaggccgcc cgctcgtcga atcggctagg
     2161 atgctccgtc ccatggcacg cgtatgtcac aactgcggta agggtccggg cttcggaaat
     2221 tcccggagtc actcgatggt cgccacccgg cgccgcttcg acccgaacct ccagaaggtc
     2281 cgcatcctcg agggaggcgc caagaagcgc gtcctggttt gtac
//
LOCUS       DAOQFI010000286         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26038_length_2320_cov_3.385872, whole genome shotgun sequence.
ACCESSION   DAOQFI010000286 DAOQFI010000000
VERSION     DAOQFI010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26038_length_2320_cov_3.385872"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLE93_05765"
     CDS             complement(<1..875)
                     /locus_tag="PLE93_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86589.1"
                     /translation="MNSVSRVVFRIVAVAALLFAGLFAASSAQAAGTPDLQLSASSSN
                     PLYGENGNVSVTAALGSGQPKGYNLSFRVVLPAGISYAGGAAYAPTVLSNSPTTGKTT
                     LIFSNISDLVEDSSQSVSFQVAHDQAQYDVGNTYDIQYEAFLNTDPRIMPAFNADGTA
                     KPASYTGSGSATTTSKINAIKISKSEPSREGEILRGVHDHQTIYTLKVQNNYVNATNG
                     TKLNDWLPADLEFLGCEGDPDNTTNAPTNPGSAEEYPGSGPIVVQPVTDCHKPSLVET
                     VQADPDGAGPMPNAV"
     gene            complement(1231..>2320)
                     /locus_tag="PLE93_05770"
     CDS             complement(1231..>2320)
                     /locus_tag="PLE93_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86590.1"
                     /translation="LDTWQGTVRNTAAGRAQAKIFAVCVRKTTGDENGHHHNLLVSAP
                     VTVTDSLVPGNNTKVLQCGPGQVAIQPGFIADKAGHLVYSQPEGNGWKFIYKVEEGGT
                     ASFSIRCMDRQVDVVNGHTHDLKLQRISTEVEVAPGTVNEAQLTCPDGSKGIVAGWDL
                     DDGLVSLGNDPRPVTRAFKLYNPTDHTLSARLSLLCLGLRTGGERVPSKTIINTANIS
                     TSSTESETGNNGSSAGINVNGIDNTPIDNDPDPDKPHPNNPIAKTIVGKGVSYSASGV
                     SFSLKCSGTCGGFAKLTTLRKLKVKGKKYGRGTVLARRQYFIGKAGTKKVKLTLTGKG
                     RKILKSGKAHRALLSISGGTRKVVKVGH"
BASE COUNT          387 a          658 c          784 g          491 t
ORIGIN      
        1 tagaccgcgt tcggcatcgg gccggcgccg tccgggtcgg cctggacggt ctcgaccagg
       61 gaaggcttat ggcaatcagt gacgggctgt acgacgatcg ggccggaacc ggggtactcc
      121 tcggccgagc cgggattggt cggcgcgttg gtcgtgttgt ccgggtcacc ctcgcaaccg
      181 aggaactcga ggtcggcggg cagccagtcg ttcagcttgg tgccattcgt cgcgtttaca
      241 tagttgttct ggaccttcag cgtgtagatc gtctggtggt cgtgaactcc gcgcaggatc
      301 tcgccctcgc ggctcggctc ggacttgctg atcttgatcg cgttgatctt cgaagtggtc
      361 gtggcagaac cgctaccggt gtagctcgct ggcttggcgg tgccgtccgc gttgaaggcc
      421 ggcatgatcc gcggatcggt gttgaggaaa gcctcgtact ggatgtcgta ggtgttgccg
      481 acgtcgtact gcgcctgatc gtgggcgacc tggaagctga ccgactggga cgaatcctcg
      541 accaggtcgg aaatgttgct gaagatcagg gtggtcttgc cggttgtcgg cgagttgctc
      601 aggaccgtgg gtgcgtaggc ggcaccgccg gcgtaggaga tcccggccgg cagcacgaca
      661 cggaaggaca ggttgtagcc cttgggctga ccggaaccga gggcggcggt gaccgagacg
      721 tttccgtttt cgccgtaaag cggattcgac gaggatgccg acagctgaag gtcgggcgtt
      781 cccgcggcct gggccgagga tgcggcgaaa agacccgcga aaagaagggc ggctacggcg
      841 acaattcgaa atacgacacg gctgacagaa ttcaattttt ctccgatact tcgtggacac
      901 cctggttggg gtccgatcca ttgctttgat ggcctgtgtg aaacggtggg aaagcctcgg
      961 aggccaggtc aactgccgca agaattgaga attgcagttg acagagcttt ttcagaatcc
     1021 cccggccgcc tgccggttct caaagggaat caagcccgtc tcccgtgttc agattggctg
     1081 tcaataatag gaacaatccg tagcttgtca agttacggtg gcgccagaag aaaaattgta
     1141 ggtgcgcggg caaccaaaat cccgctagtt gcagcaaaaa gccccggtct tgtgaccggg
     1201 gcttttactc cctggcagta agtctcaggc tcagtgcccg actttgacta cctttctggt
     1261 ccctccagaa atgctgagca gagccctgtg ggccttcccg ctcttcagga tcttgcggcc
     1321 cttgccggtc agggtcagtt tgaccttctt cgtgccggcc ttgccgatga agtactggcg
     1381 cctggcgaga actgtgccgc ggccgtactt cttgcccttg accttcagct tcctgagcgt
     1441 ggtcagcttg gcgaacccgc cacaggtacc cgagcacttg aggctgaagc tgacacctga
     1501 agcggagtag ctgacgccct tgccgacgat cgtcttggcg atcgggttgt tcgggtgcgg
     1561 cttgtccgga tccggatcgt tgtcaatcgg ggtgttgtcg atcccgttca cgttgatccc
     1621 ggccgaagag ccgttgttgc cggtttccga ctcggtcgag ctggtcgaga tgttggccgt
     1681 gttgatgatc gtcttggacg gcacccgctc accaccggta cggaggccca ggcagagcag
     1741 actgaggcgg gcactcagcg tgtggtcggt cgggttgtag agcttgaagg cccgggtgac
     1801 cggacgcgga tcgttgccga gcgaaacgag accgtcgtcg aggtcccagc cggcgacgat
     1861 tcccttgctg ccgtccgggc aggtgagctg cgcctcgttg accgtgcccg gtgcaacctc
     1921 gacctcggtc gagatgcgct ggagcttcag gtcatgggtg tgaccgttca cgacatccac
     1981 ctggcggtcc atgcagcgga tcgagaagga ggcggtgcca ccctcttcga ccttgtagat
     2041 gaacttccag ccgttgcctt cgggctggct gtagaccagg tgaccggcct tgtcggcgat
     2101 gaagccgggc tggatcgcaa cctggccggg gccgcactgc agcaccttgg tgttgttgcc
     2161 cgggaccagc gagtcggtca cggtgaccgg ggcgctgacc agcaggttgt ggtggtggcc
     2221 gttctcgtcg ccggtggtct tcctgacgca gaccgcgaag atcttggcct gggcgcgacc
     2281 ggccgcggtg ttgcggaccg tgccctgcca ggtgtccagc
//
LOCUS       DAOQFI010000287         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26197_length_2311_cov_3.097961, whole genome shotgun sequence.
ACCESSION   DAOQFI010000287 DAOQFI010000000
VERSION     DAOQFI010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26197_length_2311_cov_3.097961"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..988)
                     /locus_tag="PLE93_05775"
     CDS             complement(32..988)
                     /locus_tag="PLE93_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNH86591.1"
                     /translation="MAEVESIGADLGGTKLAVGVVDGRPEALWQEEVPSKGYSQERVV
                     NLLAQQINKAHQARPTASSVGVGIPATLDHETGHAISTVNLDLEDLPIREMLSRETRL
                     PVAIDNDGNLAMLAEALYGAARGTSNALLLTIGTGIGGGIWLNGEIYRGSTGAGAELG
                     HLVVEIDGHPCQGNCPNRGCVEAMASGTAIGRHGREAAEREPDSLLGRRLAAGEEIDA
                     KAVNEAATAGDQAAIGAIDLAGHYLGQALVGLSNVFQPEVIVLGGGAIAFGELLIAPA
                     RREVEKFALKPMNETKVVAAELGPSAGMIGAAAYGRMEAEAA"
     gene            1011..2294
                     /locus_tag="PLE93_05780"
     CDS             1011..2294
                     /locus_tag="PLE93_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNH86592.1"
                     /translation="MPSSGNKPDPDRPDYNVYRSRRGLFSRFRKPDLESLSEKEKKAA
                     DRQRAARSDQQDLPYEVQRSGSGRGGGRYGSSGLKDRFGRGGSGRSKGGKRWLKWILF
                     ACLGWFCLSVLAFAVSAQLQSMKLDGDAKAALGGSPWILTSPQNVLIIGTDSRPPDTL
                     EPGAAQEERCYEQQAEGAAPHDGCAGARADTLMVVRAGGGKFKKLSIPRDSYAAIPGQ
                     DPQKINGAYAFGGAALQIQTVEDFLGITIDHVVILNFTGFEDFIDAIGGVKVDVPVRL
                     CADISGGEANGGWSIDLEKGSNTLDGETALAYARTRKPSPCPGSEPSAYTEGYDDLDR
                     AAAQQLVMNGIKQRLTDPLRLPYNFIKGPIIGWTAPKAFVSDMGFFTLPQLALSTVFA
                     GSSKPDVLVPSGPGPGGSLEIPESERQRAAAELEG"
BASE COUNT          370 a          838 c          732 g          371 t
ORIGIN      
        1 ggggtcgggc agacggtgag gcggccgggc atcaggcggc ctcggcttcc atgcgaccgt
       61 aggcggcagc gccgatcatg ccggcggacg ggccgagctc ggcggccacg acctttgtct
      121 cgttcatcgg tttcagggcg aacttttcga cttcccgccg ggccggggcg atcaacagtt
      181 cgccgaaggc aatcgcacct ccgcccagca cgatcacctc gggctgaaag acgttcgaca
      241 ggccgaccag ggcctggccg aggtaatggc cggccaggtc gatcgcgccg atcgccgcct
      301 ggtcaccggc cgtagcggcc tcgttgactg cctttgcgtc gatctcctcg ccggccgcga
      361 ggcgccgccc aagcagcgag tcaggttccc gctcggcggc ttcgcgaccg tgcctgccga
      421 tcgccgtgcc ggaggccatc gcctcgacgc aaccgcggtt cgggcagttg ccctggcagg
      481 gatgcccgtc gatttcgacc accagatggc cgagttccgc tccggcgccg gtcgatcccc
      541 ggtagatctc gccgttcagc cagatcccgc cgccgatccc ggtgccgatc gtgagcagca
      601 gggcattgct ggtcccgcgt gcggccccgt agagcgcttc ggccagcatc gccaggttgc
      661 cgtcgttgtc gatcgccacc ggcagccggg tctcgcggga cagcatctcg cggatcggca
      721 ggtcttcgag gtcgaggttg acggtgctga tggcgtgacc ggtctcgtga tcgagcgtcg
      781 cgggtatccc gaccccgacc gaggaagcgg tcggacgggc ctgatgggcc ttgttgatct
      841 gctgcgccag caggttcacg accctctcct gcgaataacc tttggagggg acctcctcct
      901 gccacagggc ttcgggccgc ccatcgacca cgccgacggc aagctttgtc ccacccaggt
      961 cagctccgat cgattcaact tctgccatca gccaccattt ctaactcttc atgccttcct
     1021 caggtaacaa gcccgatccg gaccggcccg actacaacgt ctatcgctca cgccggggcc
     1081 tcttttcccg cttccgcaag cccgatctcg aatccctgag cgagaaggag aagaaggcgg
     1141 cggaccgtca aagggcggcc agatccgacc agcaggacct accctacgaa gtccagcgct
     1201 ccggctccgg ccggggtggc ggtcggtacg gatcttccgg cctcaaggat cgcttcgggc
     1261 gcggaggttc cggccgaagc aagggcggca agcggtggct caagtggatc ctcttcgcct
     1321 gcctcggctg gttctgcctg agcgtgctcg ccttcgccgt ttccgcccag cttcagtcga
     1381 tgaaactcga cggtgacgcc aaggccgccc tgggcggcag cccctggatc ctgaccagtc
     1441 cccagaacgt actgatcatc ggcaccgatt cccgcccccc ggacacgctc gaaccggggg
     1501 ccgcccagga ggagcgctgc tacgaacagc aggccgaggg cgcggcaccg catgacggct
     1561 gcgccggcgc ccgggccgac accctgatgg tggtccgcgc cggcggcggc aagttcaaga
     1621 agctgagcat cccgcgcgac agctacgcgg cgattcccgg ccaggatccg cagaagatca
     1681 acggcgccta cgccttcggc ggcgccgccc tccagatcca gacggtcgag gatttcctgg
     1741 gaatcacgat cgaccacgtg gtgatcctca acttcaccgg ctttgaggac ttcatcgacg
     1801 cgatcggcgg ggtcaaggtt gacgtgccgg tccgcctttg cgccgacatt tccggcggcg
     1861 aggccaacgg tggctggtcg atcgaccttg agaagggaag caacacgctc gacggggaga
     1921 ccgccctcgc ctacgcccgg acccgcaagc ccagcccctg cccgggttcg gagccaagcg
     1981 cctacaccga gggttacgac gacctcgacc gggccgccgc ccagcagctg gtcatgaacg
     2041 gcatcaagca gcggctgacc gatccgctcc gcctgccgta caacttcatc aagggcccga
     2101 tcatcggctg gaccgccccc aaggcattcg tctccgacat gggcttcttc accctccccc
     2161 agctcgccct ctccaccgtc ttcgccggct cctcaaagcc cgacgtgctg gttccctccg
     2221 gccccggccc gggcggcagc ctcgaaatcc ccgagtcaga gcgccagcgc gccgccgccg
     2281 aactcgaggg ctagaacccg ccgcgaggcg g
//
LOCUS       DAOQFI010000288         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26285_length_2307_cov_2.951599, whole genome shotgun sequence.
ACCESSION   DAOQFI010000288 DAOQFI010000000
VERSION     DAOQFI010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26285_length_2307_cov_2.951599"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /gene="cobA"
                     /locus_tag="PLE93_05785"
     CDS             complement(<1..1173)
                     /gene="cobA"
                     /locus_tag="PLE93_05785"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNH86593.1"
                     /translation="MAEVQQGKVSLVGAGPGDPGLMTARALELISEADVIFYDRLIPP
                     DALAGAREDAELIYVGKMPGTVHNPQEDINRRIVEAGREGRKVVRLKGGDPFIFGRGG
                     EEAEELADAGIPFEVVPGVTAAIAVTATAGIPLTHREDSAGVAFVTGHRDGEADAGID
                     PDGSLALFPGTLAFYMGVKRLAENAADLISGGRPADQPAAAIERGATPHQRVVTATLG
                     TIADKVEREGIRPPALIVIGDVVRHHEKLAWFEKRPLFGRTVVVTRARKQAGRLAAML
                     RGQGAEVIEMPVIESETIEASDPRIEEPFRQLREGAYDLVCFTSPNGVSAFFELIAGA
                     GLDARVLAGATVAAIGPGTARTLRPRGIEPDLVPDRAVAEGLLERLESEALEGKRVL"
     gene            complement(1173..1991)
                     /gene="hemC"
                     /locus_tag="PLE93_05790"
     CDS             complement(1173..1991)
                     /gene="hemC"
                     /locus_tag="PLE93_05790"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNH86594.1"
                     /translation="MAEALGPQAELVEVDADLPPEAGDKERFTRGVERLLLDGQAEAA
                     VHSAKDLPGEMTAGLAIGAVPARVDPRDAWIGPGGSIEEIPDGARVGTASLRRRAQLK
                     ALRPDLQMVELRGNVDTRLRKLEAGDADGLVLAMAGLQRLGRDHVVSFAFDEEQMTPA
                     AGQGCLVVQTRTGGEAQVEGISDPNSLTRLTAERAAVVGLGADCSSPVGVHARLEGNW
                     LVIDAFAGRSDGSVWVRDRVEGDPARPESLGRELAERMLRAGASEAMAGSTLSD"
     gene            complement(2039..>2307)
                     /locus_tag="PLE93_05795"
     CDS             complement(2039..>2307)
                     /locus_tag="PLE93_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNH86595.1"
                     /translation="IVARVLNENEGRWEAMNEEDRRRVETLARTIVGRMLHEPTLRLK
                     EIAGSDESFEVLATVRGLFGLDSGTDPGDGEVATVTPIRREGNS"
BASE COUNT          323 a          846 c          774 g          364 t
ORIGIN      
        1 cagcacccgc ttgccctcga gtgcttccga ctcgagcctt tccagcagac cttccgccac
       61 cgcccggtcg ggaaccagat cgggttctat gccgcgcggc cggagggtcc gggcggtgcc
      121 cggaccgatc gcggcaaccg tggcgccggc cagcaccctg gcgtccagcc cggccccggc
      181 gatcagctcg aagaaggcgg agactccgtt cggactggtg aagcagacca ggtcgtaggc
      241 accctcccgc aactggcgga acggctcctc gattcgtgga tcggaagctt cgatcgtttc
      301 cgattcgatc accggcatct cgatcacctc tgcgccctgg ccgcgcagca tggcggccag
      361 ccggccggcc tgtttgcggg ccctggtgac cacgaccgtg cgaccgaaga gggggcgctt
      421 ttcgaaccag gcgagctttt cgtgatggcg aaccacgtca ccgatcacga tcagggccgg
      481 cgggcggatg ccttcccgtt cgaccttatc ggcgatcgtg cccagggtcg cggtgaccac
      541 ccgctggtgg ggagttgcgc cgcgttcgat cgccgcggcg ggctggtcgg ccggccggcc
      601 gccactgatc aggtcggcgg cgttttcagc cagtcgcttc acccccatgt agaacgcgag
      661 ggtgcccggg aagagggcca gcgaaccgtc ggggtcgatg cccgcatctg cctcaccgtc
      721 ccggtgaccg gtgacgaagg cgacccccgc cgaatcctcg cggtgggtga gcgggatacc
      781 ggccgtggcg gtcaccgcga tcgcggcggt cacaccgggc acgacctcga aggggatccc
      841 ggcgtcggcc agctcttcgg cctcttcccc gccccggccg aagatgaacg ggtcgccgcc
      901 cttcagccgc acaactttcc ggccttcccg ccccgcctcg acgatccgcc ggttgatgtc
      961 ttcctgcggg ttgtggacgg tgccgggcat cttgccgacg tagatcagct cggcatcctc
     1021 gcgggctccg gccagcgcat ccggcggaat cagccggtcg tagaagatca catcggcctc
     1081 gctgatcagt tcgagcgcac gggccgtcat cagccccgga tcacccgggc ccgctccgac
     1141 caggctgact ttgccttgtt ggacttcggc catcagtcag acagggtaga cccggccatc
     1201 gcttcgctcg ccccggccct cagcatccgc tccgcgagtt cccggccaag cgactcgggc
     1261 cgggccgggt ccccttcgac ccggtcgcgc acccagacgc tgccgtcgga gcggccggcg
     1321 aaggcgtcga tcaccagcca gttgccctca agccgggcgt gaacaccgac cgggctcgaa
     1381 cagtcggcgc ccagaccgac caccgccgcc cgctcggccg tcagccgggt cagggagttg
     1441 gggtcggaga tcccttcgac ctgcgcctcg ccaccggtgc gggtctggac gaccaggcag
     1501 ccctgcccgg cggccggcgt catctgctct tcatcgaagg cgaagctgac cacgtggtca
     1561 cggccgagtc gctgaaggcc ggccatcgcc agcaccaggc cgtcggcatc cccggcctca
     1621 agcttgcgca accgggtgtc aacgttgccg cggagctcga ccatctgcag atccggccgg
     1681 agcgccttga gctgggctcg ccggcgcaga ctggccgtgc cgacacgggc gccgtcgggg
     1741 atttcctcga tcgaaccgcc ggggccgatc caggcgtccc ggggatcaac ccgggccggc
     1801 actgcgccga tcgcgagccc cgcggtcatc tcgccgggca ggtccttggc cgaatggacc
     1861 gcggcctcgg cctgaccgtc gagcagcagc ctttcgaccc cgcgggtgaa gcgctccttg
     1921 tccccggcct cgggtggcag gtcggcgtcg acctcgacca gttccgcctg tggtccaagc
     1981 gcctcggcca cctggccggc ctgggccagg gcgagggcac tggaacgggt tgcgaccctc
     2041 aggagttgcc ttcccggcgg atcggggtga ccgtggcgac ttcgccgtcg ccggggtcgg
     2101 tcccgctgtc gaggccgaag aggccacgga ccgtggcgag cacctcgaag gactcgtcgc
     2161 tgccggcgat ctccttcagg cgcagggttg gctcatgaag catgcgaccg acgatcgtcc
     2221 gggccagggt ctcgacccgg cgccggtcct cttcgttcat cgcctcccag cggccttcgt
     2281 tctcgtttag aacacgggcc acgatct
//
LOCUS       DAOQFI010000289         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26405_length_2302_cov_3.944370, whole genome shotgun sequence.
ACCESSION   DAOQFI010000289 DAOQFI010000000
VERSION     DAOQFI010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26405_length_2302_cov_3.944370"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /gene="ispH"
                     /locus_tag="PLE93_05800"
                     /gene_synonym="lytB"
     CDS             <1..185
                     /gene="ispH"
                     /locus_tag="PLE93_05800"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933314.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNH86596.1"
                     /translation="GVETVGITSGASAPEELVEDLVQLFRDRGVEDVSELRTVDESVR
                     FMLPKEIRKGLPMVNS"
     gene            complement(192..1043)
                     /locus_tag="PLE93_05805"
     CDS             complement(192..1043)
                     /locus_tag="PLE93_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86597.1"
                     /translation="MTEGGSYPYDHAILPLRDDIAAPELPGKATWIGKDPGKMPVLTA
                     KGPVLVHFLDYSQLNSVRTLPYLVEWHERYEPLGLRVLGVQAPRFPFGADPEAAGRGL
                     RQLGVEFPVLIDESKRLWTGYGCEGWPSLFLWGRGGILRWFHFGEGDYQGTEQAIQEA
                     LAASFERLPEMPAIMEPVRPTDAAGVEVSPPSPEVIPFPDRPLTIDDFDALALDFEGG
                     GAYVTADGKGDLRVTLDGEARGEVGIDGPGLYPLVETGRHGSHKLGIELEGAPEIWSV
                     SYSPATT"
     gene            1076..1705
                     /locus_tag="PLE93_05810"
     CDS             1076..1705
                     /locus_tag="PLE93_05810"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNH86598.1"
                     /translation="MNTNLWDRIEQARAEHNVLEHPFYQRWSAGELTREELADYSGQY
                     RYATEAIAKLSTDIAAQAPESERAGLEAHAAEEHDHVVMWDGFVGAVGGTVGAEPNPE
                     TLACVEAWTKADGFGRQLARMFAIESGQPAISKTKTEGLGEHYEITDSPGNLYFTVHE
                     EMDVHHAAEGRKLIERYMNDFSEDELVEAAEEAFKANWLLLDGVEKARA"
     gene            1861..>2302
                     /locus_tag="PLE93_05815"
     CDS             1861..>2302
                     /locus_tag="PLE93_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86599.1"
                     /translation="MAPGFGRAITSLPVRLLCGAFGLFLLGVAIYSGFKGTEAPDRNV
                     TLTLLFVTAWLGFPLLGVLVGNLFPAFNPWNAVAAPAGWAWRRVAGKAPAHLAYPERL
                     GRWPAVIGLFLFVWMELIYGEGSGVAVGVSPEAVAQAAVFYSIYT"
BASE COUNT          374 a          761 c          763 g          404 t
ORIGIN      
        1 acggggtgga aacggtcggg atcacttccg gggcaagcgc accggaggaa ctggtcgagg
       61 atctggttca gcttttccgc gaccgcgggg tggaggacgt ctccgagctc cgcaccgttg
      121 acgagtcggt ccgcttcatg ctgccgaagg agatccgcaa gggcctcccg atggtcaatt
      181 cctgaccggt ctcaggtcgt ggccggcgag tagctgaccg accagatctc cggagcgcct
      241 tccagttcga tccccagctt gtggctgccg tggcggccgg tttcgacgag cgggtaaagc
      301 cccgggccat cgatcccgac ttcgccgcgg gcttcgccgt cgagggtaac ccgcagatct
      361 cccttgccat cggcggtgac gtaggcgcca ccgccctcga aatcgagcgc cagcgcgtcg
      421 aagtcatcga tcgtgagggg gcggtccgga aacgggatca cttcgggtga gggtggcgac
      481 acctcaacgc ccgctgcgtc ggtgggccgg accggttcca tgatcgccgg catctccggg
      541 agcctctcga aggaggcggc cagcgcttcc tgaatcgcct gctcggttcc ctgatagtcg
      601 ccctcgccga agtggaacca gcggaggatt cccccgcgac cccagaggaa gaggctcggc
      661 cagccttcac atccgtaacc ggtccagagt cgcttgcttt catcgatcag gaccgggaac
      721 tcgaccccga gttgccgcag tccgcgcccg gcggcttccg ggtcggcgcc gaagggaaag
      781 cgtggagcct ggacaccgag cacccgcagg ccgagcggct cgtaacgctc gtgccactcg
      841 accaggtagg gcagggtgcg cacgctgttc agctggctgt aatccagaaa gtggaccagt
      901 acagggcctt ttgccgtgag caccggcatc ttgcccgggt ccttgccgat ccaggtcgcc
      961 ttgccgggca gttcgggagc cgcgatgtca tctcgaagag ggaggatggc gtgatcgtat
     1021 ggatatgatc cgccttcagt caccccctct acgagctcta cgaggagaga aactcgtgaa
     1081 cacgaatctg tgggaccgca tcgagcaggc ccgggccgag cacaacgtcc tggaacatcc
     1141 cttctaccag cgctggtcgg ccggcgagct gacccgcgag gaactcgccg attactcggg
     1201 ccagtaccgc tacgcgaccg aggcgatcgc caagctgagc accgacatcg ccgcccaggc
     1261 accggaatcc gagcgggccg ggctcgaggc gcatgcggcc gaagagcacg atcacgtcgt
     1321 catgtgggac ggcttcgtgg gcgcggtcgg cggcacggta ggtgccgagc cgaacccgga
     1381 gaccctcgcc tgcgtcgaag cctggaccaa ggccgacggt ttcggccgcc agctcgcgcg
     1441 catgttcgcg atcgagtcgg gccagccggc gatttcaaag accaagaccg aaggtctcgg
     1501 cgagcactat gagatcaccg actctcccgg caacctctac ttcaccgttc acgaggagat
     1561 ggacgtccat cacgccgccg agggtcgcaa gctgatcgag cggtacatga acgatttcag
     1621 tgaggacgag ctggtcgagg cggccgagga agccttcaag gccaactggc tgctgctcga
     1681 cggagtcgag aaggcgcggg cctgagcctt cccctcgcac atgtcctgtc cgccgggcag
     1741 gacctgccaa taccggcgtg gctcttcgcc tggggagcct cgattgttct cgtagtctcg
     1801 ttcttcgctc tctcagcggg ctggcgccgg gcccgctacg agagtgattc ctggcgtcag
     1861 gtggcgcccg gtttcggccg cgcgatcacc agcctgccgg tccggctcct ctgcggggcc
     1921 ttcggcctct tcctgctcgg ggtcgcgatc tattccggct tcaaaggcac cgaggcgccg
     1981 gaccgaaatg tcaccctgac cctgcttttc gtcaccgcct ggctcggctt tccgttgctg
     2041 ggagtgctgg tcgggaacct ctttcccgcc ttcaacccat ggaacgcggt cgccgccccg
     2101 gccggctggg cctggcgcag ggtcgcgggc aaggctcctg cccaccttgc ctatccggag
     2161 cggctcggac gctggccggc cgtgatcggg ctgttcctct tcgtctggat ggagttgatc
     2221 tacggtgagg gaagcggcgt ggcggtcggc gtttcacccg aggcggtcgc ccaggcggca
     2281 gtcttctact cgatctacac cc
//
LOCUS       DAOQFI010000290         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26440_length_2301_cov_2.669635, whole genome shotgun sequence.
ACCESSION   DAOQFI010000290 DAOQFI010000000
VERSION     DAOQFI010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26440_length_2301_cov_2.669635"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLE93_05820"
     CDS             complement(<1..314)
                     /locus_tag="PLE93_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH86600.1"
                     /translation="MSEARIETEVVVVGAGLAGLSAARELTRRGVEVAVVEARDRVGG
                     RILNEPIGDGEIVELGGQWVGPGQDQVLALIQELGLETFPTWCEGRNVFERGGKARTY
                     SGT"
     gene            339..1151
                     /gene="folP"
                     /locus_tag="PLE93_05825"
     CDS             339..1151
                     /gene="folP"
                     /locus_tag="PLE93_05825"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNH86601.1"
                     /translation="MKVMGIVNVTPDSFSDGGEFFDHEKAIEHGLELVRDGADVLDIG
                     GESTRPGAEEVSLEEELWRVIPVVEGLAGAAPVSIDTTKAEVAAAAIDAGAAMVNDVT
                     AFRGDMDMAAVCAGTGVEVVLMHMLGTPRTMQRDPRYRDVVAEVAEFLAVQVGLAETA
                     GVARERIWIDPGIGFGKTVDHNLSLLRWTGAFARMDLKVLVGPSRKSFIGKLSNDASE
                     SRRLGGTIAACLAAMEGGAEMVRVHDVAEVAQAIRVASAIGSANGEPGGGPE"
     gene            1161..1547
                     /gene="folB"
                     /locus_tag="PLE93_05830"
     CDS             1161..1547
                     /gene="folB"
                     /locus_tag="PLE93_05830"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNH86602.1"
                     /translation="MLQGNDGEVTIRLEGVIAHTNHGVTAAEQEVGQRMVFDLEMKPA
                     ECRATATDDVLDTVDYGEVTAWLIESAMDTRYRTLERLVTVLAEGLLENFRVDAVRLR
                     ATKPVPPVPVTMDGASVEIFRRRDEY"
     gene            1537..2067
                     /gene="folK"
                     /locus_tag="PLE93_05835"
     CDS             1537..2067
                     /gene="folK"
                     /locus_tag="PLE93_05835"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNH86603.1"
                     /translation="MSTDRPVYLGLGSNIGDRFGYLRDAIGRLEGKGLTVEAVSSAWE
                     TEPVGEILDQPDFLNAAIRVRSGLEPEELLDLLKQVEAELGRETGLPRHSPRVIDIDL
                     LLMGDLEFESDRLKLPHRETTSRRFVLAPLLELDPELALPDGTRLADAMATLESELEG
                     AEEGQGIRRAGPLLEE"
     gene            complement(2053..>2301)
                     /locus_tag="PLE93_05840"
     CDS             complement(2053..>2301)
                     /locus_tag="PLE93_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86604.1"
                     /translation="ERPELLPEYGRLFDGRSRLPKIEQERVIAAARGRYRRRRVSERE
                     SPPMRPPGVSGPPVSTNDEDPGSGTGRPGTARQASLFE"
BASE COUNT          373 a          773 c          787 g          368 t
ORIGIN      
        1 gtgcccgagt aggtgcgagc cttgccgccc cgctcgaaca cgtttctgcc ttcgcaccag
       61 gtcgggaaag tctcgagccc gagttcctga atcaacgcga gcacctggtc ctggcccggt
      121 ccgacccatt ggccacccag ctcgacgatt tcaccgtcgc cgatcggctc gttcaggatc
      181 cggcccccga cccggtcccg ggcctcgacc acggccacct cgacgccccg ccgcgtcagt
      241 tcacgggccg ccgaaaggcc ggccagcccg gcgcccacca ccacaacctc ggtctcgatc
      301 ctcgcctcac tcatgcccgg cagtctagag tcgcgtaaat gaaggtcatg ggcatcgtca
      361 acgtgactcc ggactcgttc tcggacggag gcgaattctt cgatcacgaa aaggcgatcg
      421 agcatggcct cgagctggtc agggacgggg cagacgtgct cgacatcggg ggcgagtcca
      481 cccggcccgg ggcggaagag gtttcactcg aggaagagct ttggcgggtg attcccgtgg
      541 tcgaggggct ggccggggcg gcgcccgtct cgatcgacac gaccaaggcc gaggttgccg
      601 cggcggcaat cgatgccggg gcggccatgg tcaatgacgt aacggcattc cggggggaca
      661 tggacatggc cgcggtctgc gccgggaccg gggtggaggt ggtcctgatg cacatgctgg
      721 gcaccccgag gacgatgcag cgcgacccca ggtaccggga cgtcgtcgcc gaagtcgcgg
      781 agtttctcgc ggtccaggtc ggtctggccg agacggcggg ggtcgcccgc gagaggatct
      841 ggatcgatcc cggcatcgga ttcggcaaga cggttgatca caacctgtcc ctgctcaggt
      901 ggaccggggc gttcgcccgc atggacctga aggtgctggt cggtccttcg cgcaagtcgt
      961 tcatcggcaa gctctcgaac gatgcgtcgg aatcccgtcg gctgggcggc acgatcgccg
     1021 cctgcctggc cgcgatggag ggcggggcag agatggtccg ggttcacgat gtcgccgagg
     1081 tcgcccaggc gatccgggtg gcttcggcca tcggttccgc gaacggtgaa ccgggcggcg
     1141 gcccggagta gattcccctc atgcttcagg gaaatgacgg cgaagtgacc atccgcctcg
     1201 agggcgtgat cgcccacacc aaccacgggg tgaccgcagc cgaacaggag gtcggccagc
     1261 gcatggtctt cgacctcgag atgaaaccgg ccgaatgccg ggccaccgcc accgatgacg
     1321 tgctggacac ggtcgactac ggcgaggtga ccgcctggct gatcgagtcg gcgatggaca
     1381 cccgctaccg caccctagag aggctggtca cggtgctggc cgaggggttg ctcgagaatt
     1441 tccgggttga cgccgtccgg cttcgggcga ccaagcccgt gccgccggtg ccggtgacca
     1501 tggacggcgc ctcggtcgag atcttccgtc gtcgcgatga gtactgatcg tccggtttat
     1561 ctcggactcg gttccaacat cggcgaccgg ttcggttacc tgcgcgacgc gatcggccgg
     1621 cttgaaggca agggcctgac cgtcgaggcc gtttcctccg cctgggaaac ggaaccggtc
     1681 ggcgagatcc tcgaccagcc ggatttcctc aacgccgcaa tccgggtccg cagcggtctc
     1741 gagccggaag agctgcttga cctgctcaag caggtcgaag cggaactcgg gcgcgagacc
     1801 ggcctgccaa ggcattcacc acgggtgatc gacatcgacc tgctgctcat gggtgacctc
     1861 gaattcgagt ccgaccggct gaagcttccg caccgggaaa ccacctcccg ccgcttcgtc
     1921 ctcgccccgc tgctcgagct cgacccggaa ctcgccctgc cggacggaac ccgcctggcc
     1981 gacgccatgg ccacccttga atcggagctc gaaggcgccg aagagggtca ggggatccga
     2041 cgggccggac ccctactcga agagtgaggc ctgccgggcg gttccgggcc ggccggttcc
     2101 tgagccgggg tcttcgtcat tggtcgacac cggtggtccc gatacacccg gcgggcgcat
     2161 cggcgggctc tccctttcgc tgacccgccg ccggcggtaa cggccccggg cggccgcgat
     2221 gacgcgctcc tgctcgatct tcggcagccg ggaccggccg tcaaaaaggc gcccgtattc
     2281 ggggagcagc tccgggcgct c
//
LOCUS       DAOQFI010000291         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26450_length_2300_cov_3.267261, whole genome shotgun sequence.
ACCESSION   DAOQFI010000291 DAOQFI010000000
VERSION     DAOQFI010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26450_length_2300_cov_3.267261"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1095)
                     /locus_tag="PLE93_05845"
     CDS             complement(97..1095)
                     /locus_tag="PLE93_05845"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH86605.1"
                     /translation="MKIFMTGATGFIGGATARHLVEQGHQLTCLVRDRSKAGSLGELG
                     CELVVGDLSSRPRLAEQMKGHDALLHNAALYEVGIPKDRIPALRKANVEGTVNTLEAA
                     LEAGIPRVLYVSTCAVFGNTHGEIATEDFRRPDLDSPGGLEFTSVYEETKYEAHQIAR
                     NLIETRNLPCVIAQPGGVYGPNDHSELGSTIHQFLDGKLPMVPFPEFGTGLTHVEDIA
                     AGLALILDKGEIGESYILNEGNFTMRQILEEVGGIADRKVPKRAMPTVVLKALRPVGP
                     LVGKIMGQPPNLAELIASTDGVTFWADGTKAKTELGWHPREVRTGLRQTLEAEGRL"
     gene            1169..>2300
                     /gene="hflX"
                     /locus_tag="PLE93_05850"
     CDS             1169..>2300
                     /gene="hflX"
                     /locus_tag="PLE93_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNH86606.1"
                     /translation="MTESVETSSSRRARQRAMMIAVDTDGQGTLDELGELLRTAGVAT
                     VGEVIQRRSEPDPDRYFGRGKLEEVKAEIKRTGANLVACDDELAPRQERNLEAALEVP
                     VIDRTAIILDIFADHANSAEGKLQVELAQLQYNLARMRGLWTHLERLGAGIGTRGPGE
                     SQIETDRRLARDRISDLRRKLRHVEQNRGVMRSRRQDSSLPQVALAGYTNAGKSTLLN
                     ALTGAGIGVADRLFHTLDPTTRAFEYEGRRYLVTDTVGFIKKLPHQVVEAFKATLEET
                     VLADLILHVVDASAGEDRMQEEMAAADAVLDEIGAGEKPRLLVLNKIDLLDDDQRAEA
                     RIDHPDAVQVSAVSGEGLDLLRARMERAFEETMAPVELLIPYA"
BASE COUNT          394 a          735 c          793 g          378 t
ORIGIN      
        1 gtacgggcga agcggcgaag ggtacttccg catctcgctg accacacccg acgagcgcct
       61 cagcgaggcc ctcgagcgga tgcgcgagca cctcgcctag agtcttcctt cggcttcgag
      121 ggtctggcgg aggccggttc tgacctctct cgggtgccag cccagttcgg tcttggcctt
      181 ggtcccgtcg gcccagaagg tgacgccgtc ggttgaggcg atcagctcgg ccagattggg
      241 cggctggccc atgatcttgc cgaccagggg tccgaccggt ctcaaagcct tgagaacgac
      301 ggtcggcatc gcccgcttgg gaaccttgcg gtcggcgatc ccgccaacct cctcgaggat
      361 ctggcgcatc gtgaagttgc cttcgttgag gatgtaactc tcgccgatct ctcccttgtc
      421 gaggatcagg gccaggccgg ccgcgatgtc ctcgacatgg gtcaggccgg tgccgaactc
      481 ggggaaggga accatcggca gcttgccgtc gaggaactga tggatcgtcg agccgagctc
      541 cgagtggtca ttcggcccgt agaccccgcc gggctgggcg atcacgcagg gcaggttccg
      601 ggtctcgatc aggttcctgg cgatctggtg agcctcgtac ttggtttcct cgtaaaccga
      661 ggtgaactcg aggccgccgg gactgtcgag atcgggccgg cggaagtcct cggtcgcgat
      721 ttcgccgtgc gtgttgccga agacggcgca ggtcgagacg tacaggaccc gggggatgcc
      781 ggcttcgagg gccgcttcca gggtgttgac ggtgccttcg acgtttgctt tgcgaagcgc
      841 cgggatgcga tccttgggga tgccgacctc atagagcgcg gcgttgtgga gcagggcgtc
      901 atggcccttc atctgctcgg ccagccgggg ccgcgacgaa aggtcaccga caaccagctc
      961 gcaaccgagt tcacccaggc tgcctgcctt cgacctgtcc cggaccaggc aggtcagctg
     1021 atggccctgc tcgaccaggt gcctggccgt cgcaccgccg atgaaaccgg tcgctcccgt
     1081 catgaagatc ttcatggagg ggagcgtagc gcgagcccgc gggcggcccg gagttggccg
     1141 ccgtcctgcg agttctagga tgaagtcagt gaccgagtca gttgaaacct caagcagtcg
     1201 cagggcccgg cagcgggcga tgatgatcgc cgtcgatacg gacgggcagg ggaccctgga
     1261 cgaactcggc gagctgctcc ggaccgccgg cgtcgccacg gttggcgagg tgatccagcg
     1321 ccgcagcgaa ccggatccgg accgttactt cggtcgcggc aagctggaag aggtcaaggc
     1381 ggaaatcaag cgcaccggcg ctaacctcgt cgcctgtgac gacgaactgg ctccccggca
     1441 ggaacggaac cttgaggctg ccctcgaggt gccggtgatc gaccgtaccg cgatcatcct
     1501 cgacatcttc gccgaccacg ccaattcggc cgaaggcaag ctgcaggtgg agctggcgca
     1561 gctgcagtac aacctggccc ggatgcgggg cctctggacc cacctcgagc ggctcggcgc
     1621 gggaatcggc accagggggc cgggcgaatc ccagatcgag accgaccgcc ggctggcccg
     1681 cgaccggatc tccgacctgc gccggaagct ccggcacgtc gaacagaatc gcggtgtgat
     1741 gcgaagtcgc cggcaggatt cctcgctgcc gcaggtcgca ttggccggct acaccaacgc
     1801 cggcaagtca accctgctga acgccctgac cggagccggt atcggggtgg ccgacaggct
     1861 gttccacacg ctcgacccga ccacgcgcgc cttcgagtac gaaggccggc ggtacctggt
     1921 caccgacacg gtggggttca tcaagaagct gccccatcag gtggtcgaag ccttcaaggc
     1981 aaccctcgag gaaaccgtgc tggccgacct gatcctgcat gtggtcgacg cctcggcggg
     2041 cgaggaccgg atgcaggagg aaatggctgc cgccgacgcc gtgctcgatg agatcggggc
     2101 cggtgaaaag ccccgtctgc tggtcctgaa caaaatcgac ctgctggacg atgaccagcg
     2161 ggcggaggcg cggatcgacc atcccgatgc ggtccaggtt tcggcggtca gcggggaagg
     2221 cctcgacctg ctgcgggccc ggatggagcg tgccttcgaa gagacgatgg ctccggtcga
     2281 gctgctgatc ccatacgccg
//
LOCUS       DAOQFI010000292         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26478_length_2299_cov_1.786542, whole genome shotgun sequence.
ACCESSION   DAOQFI010000292 DAOQFI010000000
VERSION     DAOQFI010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26478_length_2299_cov_1.786542"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLE93_05855"
     CDS             <1..541
                     /locus_tag="PLE93_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86607.1"
                     /translation="MHVAGSCLVKDLPAEQSYFFSKYPAVWGWATWRDAWSDYSLTTN
                     EFESVFEAFAGQFQTAEEEKYWRKTLKNYFDGGIDTWDYPWAFSIWRCQGLSIYPTKN
                     LVRNIGFSSGATHTQAWKDYRGLGDAALDSMDSIVHPPVVEINQELDRRVFNESYRQP
                     PKIVIGLKLLQRLVKNSVR"
     gene            573..1127
                     /locus_tag="PLE93_05860"
     CDS             573..1127
                     /locus_tag="PLE93_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86608.1"
                     /translation="MVAIKNTLSRIRGFRYFALIAFLVVGLYTPLGQANAGAPTVVDQ
                     YTEQIPSPGGEKTAGNHQPAGNTPNSGGDAGALSGSGSSSDGYTGEANPGGGESGNSG
                     ATKTNSSAKTGKSSREEPQGNSDKGSASSAATDREPAITSGSEAGGMGIAFPLIMVAC
                     LLCSLAVVANRRGHFNFSGDERTS"
     gene            complement(1136..1720)
                     /locus_tag="PLE93_05865"
     CDS             complement(1136..1720)
                     /locus_tag="PLE93_05865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNH86609.1"
                     /translation="MLDVGASRGQFALFSRANFPNARIICFEPQPGPAGDLRRVLGGR
                     VELIGSALGPEKGEAEMNVSARDDSSSLRAIGDRQVAEFPGTQTDHSIRVPVTTLDDV
                     LELPLERPCLLKIDVQGFELDVLRGGANTLQQVDVALIECSFIELYEGQALAEEVVTF
                     MRQAGFRLAGVHGIAYSADGSAIQADFLYRRAEA"
     gene            complement(1935..>2299)
                     /locus_tag="PLE93_05870"
     CDS             complement(1935..>2299)
                     /locus_tag="PLE93_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86610.1"
                     /translation="GPEALATEIARSHLGAVTQAYSGGEFNVPSKLMNYLAVGLPVIA
                     SVRPGSEAARIVDLAGAGWVSDSCGPEGFCDSIVEAMSDPDELSRRGEAGIAFAAKNL
                     SVNSLADKFEEAFAAVVR"
BASE COUNT          507 a          686 c          687 g          419 t
ORIGIN      
        1 gatgcacgtc gccggatcat gcctcgtcaa ggacttgccc gccgaacaga gctacttctt
       61 ctcgaagtat ccggctgttt ggggctgggc gacatggcgc gacgcgtggt ctgactattc
      121 gctgaccacg aatgaatttg aatccgtatt cgaagccttc gccgggcaat ttcagaccgc
      181 ggaggaagag aaatactggc ggaaaacgtt gaaaaattac ttcgacggtg gcattgacac
      241 ctgggactat ccttgggcgt tctcgatctg gagatgtcaa ggtttatcga tctacccgac
      301 gaagaatttg gtcaggaaca tcggattcag ttcgggtgcc actcacaccc aggcatggaa
      361 ggactaccgg ggtctgggcg acgcggcgtt ggattcgatg gactcgatag ttcatccccc
      421 ggttgttgag atcaaccagg aattggatcg tcgcgtgttc aacgaaagct atcgccagcc
      481 tcccaagatc gtgatcggac tcaaactgct gcagcgcctg gtgaagaatt cagtccgcta
      541 gagttcaagc ctgaactagg cagggaagga aattggtcgc catcaagaac acattgtcga
      601 gaatccgggg tttccgctat ttcgcgttga tcgcattcct cgtggttggt ttgtacaccc
      661 ctttgggcca ggcaaacgcg ggagcgccga cggtggtcga tcaatacacc gaacagattc
      721 cttcgcccgg gggtgagaag actgcgggca atcaccagcc cgccgggaat acgccgaatt
      781 cgggcggcga cgccggagct ctgagtggaa gcggatccag ttccgacggc tacacaggcg
      841 aggccaaccc agggggcggc gaatcaggaa attcgggagc caccaagacg aactcgtctg
      901 ccaagacggg aaagtcttcc cgggaggaac cccagggaaa ttcggacaag ggctctgcaa
      961 gcagtgctgc gactgaccgc gagccagcta tcacttcggg aagcgaggca ggtggcatgg
     1021 ggattgcctt ccccctcatc atggttgcct gcctgctgtg ttccctggcc gtcgtcgcga
     1081 accggcgggg tcacttcaac ttctccggag acgagagaac cagctaacgg ccgggtcagg
     1141 cctcggcccg tcggtagagg aaatcagcct ggatcgccga tccatcggcc gaataggcaa
     1201 tcccgtgaac gccggccagc cgaaatccgg cctgccgcat gaatgtgact acctcctcgg
     1261 ccaaagcctg cccttcgtag agttcgatga acgagcattc gatcagcgca acgtcaacct
     1321 gctggagtgt gttcgcgcca ccccgcagga catccagttc gaagccctga acgtcgatct
     1381 tgaggagaca aggtcgctcc agcggaagct ccaaaacatc atccagggtg gtcaccggaa
     1441 ccctgatcga gtggtccgtc tgggttccgg ggaattcagc tacctgccgg tcgccgattg
     1501 cccgcagcga agaggaatca tcgcgagccg agacgttcat ttcggcctca cccttctccg
     1561 gtcccagtgc ggatccgatc agttcaaccc ggcctcccag cacacgacgc aagtcacctg
     1621 ccgggccggg ctgaggctcg aaacagatga tccgagcatt ggggaaattt gctctggaaa
     1681 acagggcaaa ctgcccccgc gacgcaccga catcaagcac agtcgcgaaa ttctcgccaa
     1741 agggaattac tgagtgctca actgaagcgg ccaccccctg ccgcaatgcg ggccagtagg
     1801 cacgatttcc gagaaggcgg accagcttgc ggaccttggc cgaggccagc cggattcggg
     1861 ggcggctcac cggcagagga taccaaccgg attcaagggc gaatacagaa tcgtcgtgtc
     1921 cggcgggacc gctgctaccg aaccacggcc gcaaatgcct cttcgaattt gtcggcgaga
     1981 gaattgacgg aaaggttctt ggccgcaaaa gcgatccccg cctcaccgcg ccgactgagc
     2041 tcgtcgggat ctgacatcgc ttcgactatc gaatcgcaga agccctcggg cccgcaggaa
     2101 tcagagaccc aaccggcccc cgccaggtcg acgatccgcg ccgcttcgct ccctggtctc
     2161 acagaggcga ttaccgggag gccgaccgca aggtagttca tgagtttcga aggcacgttg
     2221 aactccccgc cagaatacgc ctgggtgacc gcaccgaggt gcgatcgggc gatttctgtc
     2281 gcaagggcct cgggcccca
//
LOCUS       DAOQFI010000293         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26490_length_2298_cov_3.302274, whole genome shotgun sequence.
ACCESSION   DAOQFI010000293 DAOQFI010000000
VERSION     DAOQFI010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26490_length_2298_cov_3.302274"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLE93_05875"
     CDS             <1..629
                     /locus_tag="PLE93_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNH86611.1"
                     /translation="PELQASAIEEYRATVGGEESGGRMLFSFDHSEFEASLGTDHSNL
                     EETARKLDQSQIDEAVAALSGASQVVIVGTDQMAFFASYLRHTLSLLDIRAEIVTSTA
                     GPSLQRLGRVDEETLLITLSSGRSHALLARAMKLAGHRGARTLAIADATLSDAVKKAD
                     MSLYFSSNSPSFLRSNTALMALVHALAYGVYSLSEDEHSERIRAYKLK"
     gene            708..1592
                     /gene="pdxS"
                     /locus_tag="PLE93_05880"
     CDS             708..1592
                     /gene="pdxS"
                     /locus_tag="PLE93_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNH86612.1"
                     /translation="MAEEATFRVKTGLAEMLKGGVIMDVVDADQAKIAEDAGACAVMA
                     LERVPADIRRDGGVARMSNPRMIKEIQDAVSIPVMAKVRIGHFVEAQVLQSLSIDFID
                     ESEVLTPADETNHVDKFGFDAPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGNVVEA
                     VRHMREIVGGIKRLSTLSSTELPTAAKELQAPLELVRDVATRGWLPVPVFCAGGIATP
                     ADAALMMQLGAEGNFVGSGIFKSDDPERRAKAIVEATTHFNDADRIAAASEQLGEAMP
                     GIEMAALGEDGRLAGRGW"
     gene            1654..2190
                     /gene="pdxT"
                     /locus_tag="PLE93_05885"
     CDS             1654..2190
                     /gene="pdxT"
                     /locus_tag="PLE93_05885"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNH86613.1"
                     /translation="MLAVQGGFQRHVEHLREAGAEPVEVRLPAELEALDGLILPGGES
                     TTITMGIEKAGLAKGILAHHQAGRPTLGTCAGMIVAGREHLGLIDITTKRNAFGRQLS
                     SFEDDISVEGVVDGPIRAVFIRAPWVESTGEGVQVLAEVRGHPVAVRQGNVTALSFHP
                     ELTPDNRIHQWFKELCVA"
BASE COUNT          407 a          776 c          734 g          381 t
ORIGIN      
        1 accccgaact ccaggcctct gcgatcgagg agtaccgcgc caccgtgggc ggggaagagt
       61 ccggcggccg catgctcttt tccttcgatc actccgaatt cgaagcttcc ctcggcaccg
      121 accactcgaa cctcgaagaa accgcccgca agctggatca gagccagatc gacgaagcgg
      181 tcgccgccct ttccggggcc agccaggtcg tcatcgtcgg caccgaccag atggccttct
      241 tcgcctctta cctccgtcac accctgagcc tgctcgacat ccgggccgag atcgtcacct
      301 cgaccgccgg cccctcgctt cagcgcctgg ggcgggtgga tgaagaaacg ctgctgatca
      361 ccctttcctc gggacgctcg catgcgctgc tcgcccgggc catgaagctg gccggccatc
      421 gcggcgcccg gacccttgcg atcgcggacg caaccctttc cgacgcggtc aagaaggccg
      481 acatgagcct ttacttctca tccaacagcc cgtccttcct gcggtcgaat accgccctga
      541 tggcgctggt tcacgcgctg gcctacggcg tctactcgct gagcgaagac gagcacagcg
      601 aacggatccg cgcttacaag ctgaagtaga cgattcgtca ggacgccacc ggcatcacct
      661 gcgtttgacc ggaccttcga gggccttctc tctctatatt ggaggggatg gctgaagaag
      721 cgactttccg agtaaagacc ggcctggcag agatgctgaa gggcggcgtg atcatggacg
      781 tggtcgacgc cgaccaggcg aaaattgccg aggacgcggg ggcctgcgcg gtcatggccc
      841 ttgagcgggt cccggccgac attcgcaggg acggcggtgt ggcccgcatg tccaacccgc
      901 ggatgatcaa ggagatccag gacgcggttt cgattcccgt gatggccaag gtccggatcg
      961 gccatttcgt cgaggcccag gtcctgcagt cgctttcgat cgacttcatc gacgagtcgg
     1021 aggtcctgac cccggccgac gagaccaacc acgtcgacaa gttcgggttc gacgcgccct
     1081 tcgtctgcgg tgcgaccaac ctcggcgagg ctctccgccg gatcggcgag ggggcggcga
     1141 tgatccgctc gaagggcgag gcgggcaccg gcaacgtcgt cgaggccgtc cgccacatgc
     1201 gcgagatcgt cggcggcatc aagcgacttt ccaccctcag ttcgaccgag ctgccgaccg
     1261 ccgccaagga actccaggct ccgctggagc tggtccgtga cgtggcgacc cggggctggc
     1321 tgccggttcc cgtcttctgc gcgggtggca tcgccacccc ggccgacgcc gccttgatga
     1381 tgcagctcgg tgcggaagga aacttcgtcg gttccggcat cttcaagtcg gatgacccgg
     1441 aacgccgggc caaggcgatc gtcgaggcga ccactcactt caatgacgca gaccgcattg
     1501 ccgctgcctc cgagcagctc ggcgaagcga tgccggggat cgagatggcc gcactcggcg
     1561 aggatggccg cctcgccgga cgcggctggt aaccaaacgg acccttcaac tgacagttcc
     1621 gcccggtccg gtgccgtccg gccccggatc ggcgtgctcg cagtccaggg cggcttccaa
     1681 aggcacgtcg agcatctgcg cgaggccggg gcggagccgg tcgaggttcg actgccagcc
     1741 gaactcgaag cgctcgacgg actgatcctg cccggtggcg aaagcaccac gatcaccatg
     1801 ggcatcgaga aagcgggcct cgcaaagggg atcctcgccc atcaccaggc cggccggccc
     1861 accctcggca cctgcgccgg gatgatcgtc gccggccgtg aacacctggg actgatcgac
     1921 atcaccacga agcgaaacgc cttcggccgc cagctttcca gcttcgagga cgacatctcg
     1981 gtcgaagggg ttgtcgacgg tccgattcgc gcggtcttca ttcgcgcccc ctgggtcgaa
     2041 tcgaccgggg agggagtgca ggtactggcc gaggtccgcg gtcacccggt ggcggtcagg
     2101 cagggaaatg tcacagccct ctcgttccac cctgaactga cccccgacaa ccgcatccac
     2161 caatggttca aagaactctg tgttgcctga agccggagcc agcccatcaa tctgaagctt
     2221 ctgtgggctg gcgggtttga ctgggagggc gggtccgacc ccgcccgact tgcagttaag
     2281 aattcgggag gggtgagc
//
LOCUS       DAOQFI010000294         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26531_length_2296_cov_3.490852, whole genome shotgun sequence.
ACCESSION   DAOQFI010000294 DAOQFI010000000
VERSION     DAOQFI010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26531_length_2296_cov_3.490852"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLE93_05890"
     CDS             complement(<1..323)
                     /locus_tag="PLE93_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86614.1"
                     /translation="MTDVIQTVGSYAGLVALLAIGVLAFLVFSQARDIRRLREWAGGA
                     PERDAEVREVSEIVAEERSAELRVLADREERRMERAGLAEPSFWDRLGRSGRIIAIVA
                     AVLILG"
     gene            complement(444..2288)
                     /locus_tag="PLE93_05895"
     CDS             complement(444..2288)
                     /locus_tag="PLE93_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNH86615.1"
                     /translation="MQMAGDGLLVALSYFLAFQLRFLDDPGTIPERYVDLFLQSVGFV
                     IVGKLIVFYLFGLYQKWWRFVGGRDIARIVQAVSVSSLLLVVLFTVIQPFPHDLPRSV
                     AVTDFLLTLFLIAGARLATRLFKERPNKGNRIARGREVLVVGAGSGGQMVVRELRINP
                     NLGETAIGFVDDNTEMRGMRIQGIRVLGTTDEIETILDETEPDEVMIAIPSAPGVLRA
                     RVVAACRERQIRVRTLPTVFELLRGGVQLSQQLREVQVEDVLGRDPVTMELARVGAYL
                     EDQVVMVTGAGGSIGKELCRQIGLVGPRLLVMLDHAEDNLFQIEREMVAERHFTRVEA
                     VLADCKETERIFEVMQRFKPNIVFHAAAYKHVPLMESNPLEAVRNNSFATRITADCAA
                     AAGAERFVLISTDKAVNPQTVMGASKAMAEWAVAAADAHHANTRFVSVRFGNVLGSSG
                     SVVPIFKRQIEQGGPVTVTHPEMTRFFMTIPEAVQLVIRAGDTGVSRGDVFVLDMGEP
                     VKITDLAENMIKLAGREPEKDIAIEFTGPRPGEKLHEELFGVEERPQPTASKRIMRAV
                     REEPIDPEWIVQTLDRLDQVVQTGDEANLAETVVELASNPGSDPAKVI"
BASE COUNT          382 a          773 c          759 g          382 t
ORIGIN      
        1 ccgaggatca gcaccgccgc gacgatcgcg atgatccggc cgctgcggcc gaggcgatcc
       61 cagaaggaag gctcggccag tccggcccgc tccatgcgcc gctcctcgcg gtcggccagc
      121 accctgagct cggccgagcg ctcttcggcg acgatctcgc tgacctcgcg aacctcggca
      181 tcccgctctg gtgcaccgcc ggcccattcg cgcagacggc ggatgtccct ggcctgggag
      241 aacacgagga aagcgagcac gccaatcgcc agcagggcga cgagcccggc gtaggagcct
      301 acggtctgga ttacgtcggt caactgatct ccacttttct ggacgtcagg gcgtagttcc
      361 cggggctggt gggcccacga cccggatacg ccgaccgggg catgttattt gggtttggcg
      421 gatgaatgcg ccttgagccg gtttcagatg acctttgccg gatcgctgcc ggggttgctg
      481 gccagttcga ccacggtctc ggcgaggttc gcttcatccc cggtctgaac gacctgatcg
      541 agtcgatcga gggtctgaac gatccattcc gggtcgatcg gttcctccct gaccgccctc
      601 atgatccgtt tcgaagccgt cggctgcggc cgctcttcca ccccgaacag ctcttcgtgg
      661 agcttctccc ccggccgggg cccggtgaac tcgatcgcga tgtccttctc cggctcgcga
      721 ccggccagct tgatcatgtt ctcggccagg tcggtgatct tgaccggctc gcccatgtcg
      781 agcacgaaga cgtcaccccg gctgaccccg gtgtcgccgg cccggatgac cagctggacc
      841 gcttccggga tcgtcatgaa gaagcgggtc atctccgggt gcgtgaccgt gaccggaccg
      901 ccctgctcga tctggcgctt gaagatcggc accacgctgc cggaggatcc gagcacgttg
      961 ccgaaacgga ccgagacgaa gcgcgtgttc gcgtggtgcg cgtcggcggc ggcgaccgcc
     1021 cactcggcca ttgcctttga ggcacccatc acggtctgcg ggttgaccgc cttgtcggtc
     1081 gagatcagga cgaagcgctc ggccccggcc gcggcggcgc aatcggcggt gatccgggtc
     1141 gcgaacgagt tgttgcgaac cgcctcgagc gggttcgact ccatcagcgg cacgtgcttg
     1201 taggcggcgg cgtggaagac gatgttgggt ttgaagcgct gcatcacctc gaagatccgc
     1261 tcggtctcct tgcagtcggc caggaccgcc tcgaccctgg tgaagtggcg ctcggccacc
     1321 atctcgcgtt cgatctggaa gaggttgtcc tcggcgtggt cgagcatgac cagcagccgc
     1381 ggtccgacca gcccgatctg gcggcagagc tccttgccga tcgagccgcc cgcgccggtc
     1441 accatcacga cctggtcctc gaggtaggcg ccgaccctgg ccagctccat ggtcaccgga
     1501 tcgcggccga gcacgtcctc gacctggacc tcgcgcagct gctggctgag ctggacgccg
     1561 ccccgcagca gctcgaacac ggtcggcagg gtgcggaccc ggatctgccg ttcacggcag
     1621 gcggccacca ccctcgcccg cagcacaccg ggcgcggagg ggatcgcgat catcacctcg
     1681 tccggttcgg tttcgtcgag gatcgtctcg atctcgtcgg tggtgccgag cacccggatg
     1741 ccctggatgc gcatcccgcg catctccgtg ttgtcatcga cgaagccgat cgcggtctcg
     1801 ccgaggttcg ggttgatccg cagttcgcgg accaccatct gaccaccgga accggccccg
     1861 accaccagta cctcgcggcc ccgggcgatc cggttgccct tgttcggacg ttccttgaag
     1921 agccgggtgg cgagccgggc gccggcgatc agaaagaggg tgaggagaaa gtcggtcacc
     1981 gcgaccgagc ggggcaggtc atgcgggaag ggctggatca ccgtgaacag cacgaccagc
     2041 aggaggctcg aaaccgaaac cgcctggacg atccgggcga tgtcacgacc gcccacgaaa
     2101 cgccaccact tctggtagag cccgaacagg tagaagacga tcagcttgcc gacgatcacg
     2161 aaaccgaccg actgcaggaa caggtcgacg taacgctcgg ggatcgtccc cgggtcatcg
     2221 aggaagcgaa gctggaaggc gaggaagtag gaaagcgcga cgagcaggcc gtcaccggcc
     2281 atctgcatca gctggt
//
LOCUS       DAOQFI010000295         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26585_length_2294_cov_2.869585, whole genome shotgun sequence.
ACCESSION   DAOQFI010000295 DAOQFI010000000
VERSION     DAOQFI010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26585_length_2294_cov_2.869585"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLE93_05900"
     CDS             complement(<1..312)
                     /locus_tag="PLE93_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86616.1"
                     /translation="MTGRKCALWLAAAISVLTFATAATANGATRERPCNGAVELCSKT
                     LDQVVLPGTHNSMSNEEYGWILPNQHYAIPTQLEYGVRAFLIDTHYGKPSAGSGPSNV
                     AD"
     gene            376..1416
                     /locus_tag="PLE93_05905"
     CDS             376..1416
                     /locus_tag="PLE93_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH86617.1"
                     /translation="MKSQGVMGVVQKLVGLAIGLLFAMPDSLMRKIFGDPPAAVAALR
                     PDAWAMGRLIGLAEDKRSEWNPEEAREETEFLAGAVSRPVTGVITQDWLLEAGDHSLP
                     ARLFTPGANPLAGPMLVHFHGGGWVQGSINSHHGACAWLAREAGVRVLSVDYRLAPEH
                     PFPAQADDALAAWRSVMSDPARFGADPEKVGVIGDSAGAQMCAVLCLDLKAAGEPQPA
                     CQVLIYPVTDCTGSFPSRESFARGFYLTRARMDWFEDCFVPEGEAENPRVSPLFADDL
                     SGLAPALVSVSLADPLRDEGVAFAGRLIEAGVPVTLDTMPMLHAWFNLTSSRSSRQGH
                     RILAGRITDLLG"
     gene            1450..>2294
                     /locus_tag="PLE93_05910"
     CDS             1450..>2294
                     /locus_tag="PLE93_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNH86618.1"
                     /translation="MPEAAANITVSLPDGTPLELDPGATGADAALAIGEGLARAALAL
                     KVGDEVRDLSAPVSDGEEISIITSRDPEGLELIRHDAAHVMATAVQELYPGTKVTIGP
                     AIENGFYYDFEFPEGVKITDAELPAIEEAMRRHIKADETFERRDVPVAEAIEIFKAQD
                     ERFKVELIEDLIENEGAETVSLYRNGPFEDLCRGPHGPGTKRIKVIKLNSVAGSYWRG
                     DENREQLTRIYGTAFFSKDDLAEYLERIEQAKARDHRRLGPELDLFMLRDEAPGMPFW
                     LPNGTV"
BASE COUNT          390 a          757 c          752 g          395 t
ORIGIN      
        1 atccgcgaca ttcgaggggc cagatcctgc ggaaggcttg ccgtaatgcg tgtcgatcag
       61 gaacgcccgc acgccgtact cgagctgggt cggaatcgcg taatgctggt tgggcaggat
      121 ccagccgtat tcctcgtttg acatcgagtt gtgggtgccc ggcaggacca cctggtcgag
      181 cgtcttcgag cagagctcga ccgcgccgtt gcacggtcgc tctcgcgttg cgccgttcgc
      241 cgtggcggcg gtagcgaagg tcaggacact gatcgctgcg gcgagccaca gcgcacactt
      301 tctcccggtc attgaattca tcatctcaac taaccgcgaa gtgaagtcgc agttgcggcc
      361 aatacgatgc gctccgtgaa gtctcaaggg gtgatggggg tcgttcagaa gctggtcgga
      421 ctcgcgatcg gcctgctctt cgcgatgccg gattcactga tgcggaaaat cttcggtgac
      481 ccgccggccg cggtcgccgc gctgaggcct gacgcctggg cgatgggaag gctgatcggc
      541 ctggccgagg acaagcgcag cgaatggaat cccgaagaag cgcgagagga aaccgagttc
      601 ctggccggcg cggtcagccg tccggtcacc ggcgtgatca cccaggattg gctgctggag
      661 gccggggatc actcactgcc cgcccgcctc tttactcccg gggccaatcc gctggccggg
      721 ccgatgctgg ttcactttca cgggggcggt tgggtccagg gctcgatcaa ctcccatcac
      781 ggagcctgtg cctggcttgc ccgcgaagcc ggggtccggg ttctttcggt tgattacagg
      841 ctggcgccgg agcacccttt cccggcccag gccgatgacg cgctggccgc ctggcgatcc
      901 gtgatgagcg acccggcccg cttcggggcc gacccggaga aggtgggggt gatcggcgac
      961 agcgccggtg cccagatgtg cgcggtgctc tgcctcgacc tgaaagcggc gggggagccg
     1021 cagcccgcct gccaggtcct gatctacccg gtcaccgact gcaccgggtc gttcccctcc
     1081 cgggaatcgt ttgccagggg cttctacctg accagggccc ggatggactg gttcgaggac
     1141 tgcttcgtgc ccgaaggaga agcggaaaac ccgcgcgtct cgccactctt tgcggatgac
     1201 ctttccggtc ttgccccggc gctggtttcc gtgtcgctgg ccgatccgct gcgggatgaa
     1261 ggagtcgcct tcgccgggag gttgatcgag gccggggtcc cggtcaccct cgacacgatg
     1321 ccgatgcttc acgcctggtt caacctcacc tccagccgtt cctcccgcca gggccaccgg
     1381 attctggccg ggcggatcac cgatctgctc ggctgatccc gaggtccccg ccttcaatac
     1441 gattgaccga tgccggaagc cgccgcaaac atcaccgtat ccctgcctga cggcaccccg
     1501 ctcgagctcg acccgggcgc gaccggtgcc gacgcggccc tggcgatcgg cgaaggcctg
     1561 gcccgggccg cgctggcact caaggtcggg gacgaggttc gtgatctcag cgccccggtc
     1621 agtgacggcg aggagatctc gatcatcacc agccgtgacc ccgagggtct cgagttgatc
     1681 cgccacgatg ccgcgcacgt gatggcgacc gcggtccagg agctctaccc gggcaccaag
     1741 gtgacgatcg gtccggcgat cgagaacggc ttctactacg acttcgagtt ccccgagggc
     1801 gtgaagatca ccgacgccga actgccggcg atcgaagaag cgatgcgccg ccacatcaag
     1861 gccgacgaaa ccttcgagcg tcgcgacgtg ccggtcgccg aggcgatcga gatcttcaag
     1921 gcccaggatg agaggttcaa ggtcgaactg atcgaggacc tgatcgaaaa cgaaggcgcc
     1981 gagaccgttt cgctttaccg caacggtccc ttcgaggatc tctgccgcgg gccccacggt
     2041 cccggaacca agcggatcaa ggtgatcaag ctgaactcgg tcgccggttc ctactggcgc
     2101 ggcgacgaga accgcgagca actgaccagg atttacggca ccgccttctt cagcaaggac
     2161 gacctcgccg aataccttga gcggatcgag caggccaagg cccgcgacca tcgccggctc
     2221 ggccccgaac tcgacctttt catgctgcga gacgaggcac cgggcatgcc cttctggctg
     2281 ccgaacggca ccgt
//
LOCUS       DAOQFI010000296         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_26767_length_2286_cov_2.425818, whole genome shotgun sequence.
ACCESSION   DAOQFI010000296 DAOQFI010000000
VERSION     DAOQFI010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26767_length_2286_cov_2.425818"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PLE93_05915"
     CDS             complement(<1..506)
                     /locus_tag="PLE93_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNH86619.1"
                     /translation="MSTTEQAVRIETGTYAVDPVHTQVGFEVKHLGISTFRGSFGGFE
                     GNITVGDEGIVAIEGSIDVSTVAVPEEQLIGHLLSPDFFDAENHPKGTFVSTGVSLVD
                     AETYEVTGNLTLRGVTNPVTLTVEVEGAGIGMDGSSVLSLKANGEVNRNDYGISWGAS
                     LDNGAAVVA"
     gene            624..1133
                     /locus_tag="PLE93_05920"
     CDS             624..1133
                     /locus_tag="PLE93_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNH86620.1"
                     /translation="MTQVTAKDPGHETGSKSAHEAAFQSMRSAYLLLSERFEKELAEA
                     GLPDLGFYGVLLALSEAPEPSRPRDLLCKVNVTKSGLTRLVDRIEKAGLVERTYCPSD
                     RRGTFLVITEEGRETLARMQPVRERVFTEGFIDLVDEEDAETVARVMESVSQGIYESL
                     NTDGACDEA"
     gene            complement(1224..>2286)
                     /locus_tag="PLE93_05925"
     CDS             complement(1224..>2286)
                     /locus_tag="PLE93_05925"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNH86621.1"
                     /translation="EDEEELARVVAENTIFCRIRPEQKRQLVSALAESGAYTAMIGDG
                     VNDVPALKSARMAVAMGSGSQVTKSVADVVLLKDQFSRLPEAIAEGRRIARNIHRLGR
                     LYLTKTVYAAALIILVSVPGFTFPFLPRHLTLAAFLTIGIPSFVLALAPSDGPLYRGR
                     LLRALGAFAIPAGLATALSSILSFFLVDIAFGGSLEDGRTAATTTLIILGLCFVLLLE
                     RGPGREHIAIQSYMLAMVAGLGALFAGILAVPQLREFFDMTMLGAEQWFIAMLSAALG
                     LVFASLMWRLPQIQAWEVPDEDSFPPFERTPPPDNDRRPMPGPTHHRPRPEESPTEVI
                     PAEADLLDEPPQNGAPTNH"
BASE COUNT          415 a          691 c          795 g          385 t
ORIGIN      
        1 gcaacgacgg cggcaccgtt gtcgagcgag gcgccccagg agatcccgta atcgttgcgg
       61 ttgacttcac cgttcgcctt gaggctgagg acggagctgc cgtccatgcc gatgccggcg
      121 ccctcgacct cgacggtcag cgtcaccggg ttggtcacgc cgcgcagggt caggttgccg
      181 gtgacctcat aggtctcggc gtcgaccagg cttacgccgg tcgaaacgaa ggtgcccttg
      241 gggtgattct ctgcgtcgaa gaagtcgggg ctgagcaggt ggccgatcag ctgctcttcg
      301 ggaacggcga cggtggagac gtcgatcgaa ccctcgatcg cgacgattcc ctcgtcaccg
      361 acggtgatgt tgccctcgaa gccgccgaag ctgccgcgga aggtggagat gccgaggtgc
      421 ttcacctcga agccgacctg ggtgtggacg gggtcgacgg cgtaggtgcc ggtctcgatg
      481 cgtactgcct gttcggtggt gctcattttg tttcccttcg tttcaattca agttgactga
      541 gaaattgttg cgtaagcaac tgttgtacgg acaccatagc aaaaatagtt gcgctgtcaa
      601 ctacttgcgc tacaatgtgc tctatgacac aggtgacggc aaaagacccg gggcacgaga
      661 ccggctcgaa atcggctcac gaagccgcat tccagtcaat gcgcagcgcc tacctgctgc
      721 tttcggagcg cttcgagaag gaactggccg aagccggtct gcccgacctc gggttctacg
      781 gagtgctgct ggcgctgagc gaagctcccg agccgagccg gccccgcgac ctgctctgca
      841 aggtcaacgt gaccaagagc ggactgaccc gcctggtcga ccggatcgag aaggcggggc
      901 tggtcgagcg cacctactgc ccctcggatc gccgcggcac cttcctcgtc atcaccgaag
      961 agggccgcga gactctggcc aggatgcagc ccgtccgcga acgggtcttc accgaaggtt
     1021 tcatcgacct ggtcgatgaa gaagacgcgg agacagtggc ccgggtgatg gaaagcgtct
     1081 cccagggcat ctacgagtca ctgaacaccg acggcgcctg cgacgaggcc tgactgcgag
     1141 tcgggcgggg tccgaaaatg actgcagtcg gtccggtctt cgccggacct ccaatcggaa
     1201 cccgctccgc ccactagaaa tatctaatgg ttggtcggag ctccgttttg tgggggttcg
     1261 tccagcaggt cggcttcggc ggggatcact tcggtgggag attcctcggg ccggggccgg
     1321 tggtgggtcg gaccgggcat tggcctgcgg tcgttatccg ggggaggggt tcgttcgaag
     1381 ggcgggaacg aatcctcgtc cggaacctcc caggcctgga tctggggcag gcgccacatc
     1441 aggctggcga agaccaggcc gagcgcggcc gaaagcatgg cgatgaacca ctgttcggcc
     1501 cctagcatcg tcatgtcaaa gaactcgcgc agctggggca cggccaggat cccggcgaac
     1561 agcgctccga gcccggcgac catggccagc atgtagctct ggatcgcgat gtgctcgcgg
     1621 cccgggccgc gctccagaag cagcacgaag cagagaccga ggatgatcag ggtggtcgtc
     1681 gcggcggtgc gtccatcctc gaggctgccg ccgaaggcga tgtcgacgag gaagaacgag
     1741 agaatcgagg aaagcgcggt cgccaggccg gccgggatcg cgaaggcccc gagcgccctg
     1801 agcagcctgc cgcggtagag cggcccgtcc gacggcgcga gggcgaggac gaatgagggg
     1861 atgccgatcg tcaggaaggc ggccagggtg agatgccgcg gcaggaaggg aaaggtgaag
     1921 ccgggtaccg agaccaggat gatcagggcc gccgcgtaga cggtcttggt caggtagagg
     1981 cggccgaggc ggtgaatgtt gcgggcgatc cggcggccct cggcgatcgc ttccggcagg
     2041 cggctgaact ggtccttgag cagcaccaca tcggccaccg acttggtcac ctggctgccg
     2101 gagcccatcg cgacggccat ccgggcgctc ttcagggccg gcacgtcgtt gacgccgtca
     2161 ccaatcatcg ccgtataggc gccggactcg gcaagtgccg aaaccagctg gcgcttctgt
     2221 tcggggcgga tccggcagaa gatcgtgttt tccgcgacga cccgggccag ctcctcctcg
     2281 tcttcg
//
LOCUS       DAOQFI010000297         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27190_length_2266_cov_3.177748, whole genome shotgun sequence.
ACCESSION   DAOQFI010000297 DAOQFI010000000
VERSION     DAOQFI010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27190_length_2266_cov_3.177748"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /gene="ychF"
                     /locus_tag="PLE93_05930"
     CDS             <1..858
                     /gene="ychF"
                     /locus_tag="PLE93_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNH86622.1"
                     /translation="AGIVKGASEGEGMGNKFLSNIRESDAICQVTRVFRDDDITHVDG
                     EVEPKNDIEVIRTELILADLQTIENALPRLTKEAKRDKELEPQVKAAGEAKEILEGGK
                     GIFEAGFDPEQISDLHLMTAKPLLYVFNCSEDELADESLKDELRQLVAPAEAIFLDAK
                     FEAELVELDDEEEAREMLADIGVEEPGLDVLARVGFETLGLQTYLTAGPKETRAWQIH
                     KGDTAPQAAGVIHTDFQRGFIKAEIISFDDLLEFGSIADARAAGKVRLEGKDYVMQDG
                     DVVDFKFNV"
     gene            932..1186
                     /locus_tag="PLE93_05935"
     CDS             932..1186
                     /locus_tag="PLE93_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86623.1"
                     /translation="MPAAYQAGDLQQIFQRINDRIRAIEEHLVVLSEKAGVAYSLPGE
                     GLPKEVIELARAGKTLEAIKLYRELTNADFETAKAAVQAV"
     gene            complement(1190..>2266)
                     /locus_tag="PLE93_05940"
     CDS             complement(1190..>2266)
                     /locus_tag="PLE93_05940"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HNH86624.1"
                     /translation="TVGLPAKSGVAGGIIAVLPGQLGVAVYSPPLDLHGNSVRGVKVC
                     EDLSDELHLHVMSPARRPPPPVRIERTCIDSRSKRLRGPAEQQILAEHGRRVKVLELQ
                     GELSFAAGELVTSSALHRAEHHDFTVLDFRAVRAIDLIDVPIFSHLVESCEEAGSEIA
                     FSDVDRHPVFLEELTRHREREGHHPLRTFPDLDLALEWCEQGLIEKYGDLESDPELSL
                     AQHGTTLGMSVEEIEELSSHLEHVSYEAGEMIYGAELVAPDQMLLVTAGKISLLAEDD
                     EGGHRRLATLSPGMTLGEISMIAGTPLAGMPRADTEVKAWVLTAGDLEVLRARNPGLV
                     VTLLENLLRQVAQRVGHLRAELLR"
BASE COUNT          414 a          745 c          747 g          360 t
ORIGIN      
        1 gccgggatcg tcaagggtgc ttcggagggc gagggcatgg gcaacaagtt cctttcgaac
       61 atccgcgaat cggatgcgat ctgccaggtg acccgggtct tcagggatga cgacatcacc
      121 catgtcgacg gcgaagtcga accgaagaac gacatcgagg tgattcgcac cgagctgatc
      181 ctcgccgacc ttcagacgat cgagaacgcg ctgccgaggc tgaccaagga agccaaacgg
      241 gacaaggagc tcgaaccgca ggtcaaggcg gccggggagg cgaaggagat tctcgaaggc
      301 ggcaagggga ttttcgaggc cggcttcgac ccggagcaga tcagcgacct tcatctgatg
      361 accgccaagc cgctgcttta cgtcttcaac tgctccgagg acgagctcgc cgacgagtcg
      421 ctgaaggacg agctgcgcca gctggtcgcc ccggccgagg cgatcttcct cgacgccaag
      481 ttcgaggccg aactggtcga gctcgatgac gaggaggaag cccgcgagat gctcgccgac
      541 atcggggtcg aggaaccggg cctcgacgtg ctggcccggg tcgggttcga gaccctcggc
      601 ctgcagacct atctcaccgc cggcccgaag gaaacccgcg cctggcagat ccacaagggc
      661 gacaccgctc cgcaagccgc cggcgtcatc cacaccgatt tccagcgcgg cttcatcaag
      721 gccgagatca tctccttcga cgacctgctc gagttcggtt cgatcgccga cgcccgggcc
      781 gccggcaagg tccgcctcga aggcaaggac tacgtgatgc aggacggcga cgtggtcgac
      841 ttcaagttca acgtctgacc gcgaaagcgc ccggcttccg gacaccccgg ttatgctcgg
      901 ctgacatcca gtcaatcgaa gggggaagtc aatgccagct gcctaccagg ccggagatct
      961 ccagcagata ttccagcgga tcaacgaccg catccgggcg atcgaggagc acctggtcgt
     1021 gctttcggag aaggccgggg ttgcctactc gctgcccggc gaaggcttgc cgaaggaagt
     1081 gatcgaactg gcccgcgccg gcaagaccct ggaagcgatc aagctctacc gcgagctgac
     1141 aaacgccgac ttcgagaccg cgaaggcggc cgttcaggcg gtctgacggt cagcgcagga
     1201 gctcggcgcg aaggtgaccg acccgctggg cgacctgcct cagcaggttc tccagcaggg
     1261 tcacgaccag ccccgggttc ctcgcccgca acacctcgag gtcgcctgcg gtcagaaccc
     1321 acgccttgac ctcggtgtcg gcgcggggca tgccggccag cggcgtcccg gcgatcatcg
     1381 agatctcgcc cagggtcatc cccggtgaga gcgtcgccag gcgccggtgc ccgccttcat
     1441 cgtcctcggc cagcaggctg atcttccctg ccgtgaccag cagcatctgg tcgggtgcga
     1501 ccagctccgc tccgtagatc atctcccccg cctcgtagga aacgtgctcg aggtgtgaac
     1561 tcagctcctc gatctcttcg acgctcatgc cgagggtcgt accgtgctgg gcgaggctga
     1621 gttccgggtc ggattcgagg tcgccgtact tctcgatcag cccctgctcg caccattcga
     1681 gggcgaggtc gaggtccggg aaggtcctga gcgggtggtg gccttcgcgt tcccgatgcc
     1741 gggtcagctc ttccagaaag accggatggc ggtcgacatc cgagaaggcg atttcactgc
     1801 cggcctcctc gcagctctcg accaggtgac tgaagatcgg cacgtcgatt aggtcgatcg
     1861 ccctgaccgc ccggaagtcg agcaccgtga agtcgtggtg ttcggcgcgg tggagggccg
     1921 aactggtgac cagctcgccg gccgcgaagg aaagctcgcc ctgcagttcg aggactttca
     1981 cccggcggcc atgctcggcg aggatctgct gctcggccgg gccccgcaaa cgctttgacc
     2041 gcgagtcgat gcaggtccgc tcgatcctga ccggaggcgg tggccgccgg gccgggctca
     2101 tcacatgcag gtgaagctcg tcggagaggt cttcgcagac cttgaccccg cgaacgctgt
     2161 tgccgtgcag gtcgagcggc ggcgagtaga cggccacccc gagctggccg ggcagaaccg
     2221 cgatgatccc gccggccacg ccggacttcg ccggcaggcc gaccgt
//
LOCUS       DAOQFI010000298         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27218_length_2265_cov_2.862896, whole genome shotgun sequence.
ACCESSION   DAOQFI010000298 DAOQFI010000000
VERSION     DAOQFI010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27218_length_2265_cov_2.862896"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLE93_05945"
     CDS             <1..406
                     /locus_tag="PLE93_05945"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNH86625.1"
                     /translation="EVATACLAGRAPQIRSDGSPERDYLHVSDAAAAYLAIEGAVKDG
                     PGRGEAFNAGTGEPWPVRTIVELIADEAAREGAELVEPEFLGDGVPPGEIDRQFVDAT
                     KLTELTGWKPEMTLGAGITEAVEWYRNHGNGR"
     gene            443..1153
                     /locus_tag="PLE93_05950"
     CDS             443..1153
                     /locus_tag="PLE93_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNH86626.1"
                     /translation="MCGRYTLTSSDQRRLGSRFQISLDDESPGLGRYNVAPTQEVLTV
                     NTHEGSGEREAVASRWGLVPFWAKDLKAGYKMINARNDRLLQSKAYGPLVKKSKNRCL
                     IVADGFYEWLKAEKPKEPRQPVRFTVDGGEPFAFAGLTVSREWEGEPMTSCTIITTDA
                     NDVVSQVHDRMPVILPNEEVEQAWLTGDLSPEEAVSLCVPLDPKRMEAKPANPVLNKV
                     GGAKEGPDFLEAPEKNSD"
     gene            complement(1157..1966)
                     /locus_tag="PLE93_05955"
     CDS             complement(1157..1966)
                     /locus_tag="PLE93_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86627.1"
                     /translation="MTDPAPQQTPDESAKRDRIPRLAIAVVVVGLLATAAVYAIAQLP
                     GSGSLEIDWDTTEVVSTPPARDLGNGSFAVARSSLSALAPNDEGGLIYRVAGVVRIDS
                     GGRKLTTVRCDVNSNAAGETNRMAHSSRLRAAWPKPSDALQEQEVPETSFVKFRTPDS
                     KKIDLPIRDVVQRYIDTDAPTQVEWDGYVEDDQNWIWTMPNGTGVGAATLPWLVIFKT
                     DARPKGTIKCKATVGDKSARVLIPFRQDEWPIADDQPNTDEAGTGDVTNVQ"
BASE COUNT          405 a          693 c          771 g          396 t
ORIGIN      
        1 cgaggtggcg accgcctgcc tggccgggcg cgcgccgcaa atccgctccg acggctcgcc
       61 cgaacgggac tacctgcatg tcagcgacgc ggccgccgcc tacctggcga ttgaaggtgc
      121 ggtgaaagac ggtcccggtc gcggcgaagc cttcaacgcc ggaaccggcg aaccctggcc
      181 ggtacggacg atcgtcgagc tgatcgccga cgaagccgcc cgcgaaggtg ccgaactggt
      241 cgagcccgaa ttcctgggtg acggcgtgcc cccgggtgaa atcgaccgcc agttcgtcga
      301 cgcgaccaaa ctgaccgaac tcaccggctg gaagcccgag atgaccctcg gcgccggaat
      361 caccgaagcg gtcgagtggt acaggaacca cgggaatggc cgttgacatg cgggcgattt
      421 ccgccctgtt aggttggggg ccatgtgcgg cagatacaca ctgacttcat ccgatcagcg
      481 gcggctcgga agccggttcc agatttcact tgatgacgag tcgcccggtc tcgggcgcta
      541 caacgtggcg ccgacccagg aagtgctgac cgtcaacacg cacgaagggt ccggcgagcg
      601 cgaagcggtt gcctcgcggt ggggtctggt tccgttctgg gccaaggacc tcaaggccgg
      661 ctacaagatg atcaacgccc gcaacgaccg tttgcttcag agcaaggcct acggtccgct
      721 ggtcaagaag agcaagaatc gctgcctgat cgtggccgac ggtttctacg aatggctgaa
      781 ggcagagaag ccgaaggagc cgcgccagcc ggtccgtttc acggtcgacg ggggagagcc
      841 tttcgctttc gccggtttga cggtttcccg ggagtgggaa ggggagccga tgacctcctg
      901 cacgatcatc accaccgacg ccaatgacgt ggtcagtcag gttcacgacc ggatgccggt
      961 gatcctgccg aacgaagagg ttgagcaggc ctggctgacc ggtgaccttt ctcccgagga
     1021 ggcagtttcg ctctgcgtgc cgctcgaccc gaagcggatg gaggcgaaac cggccaaccc
     1081 ggtgctgaac aaggtcggcg gggcaaaaga aggccccgac ttcctcgagg ccccggaaaa
     1141 gaactccgac tagccgctac tggacgttgg tgacgtcgcc ggtgccggcc tcgtcggtgt
     1201 tcggctgatc gtcggcgatc ggccattcgt cctgacggaa ggggatcagg acccgggccg
     1261 acttgtcacc aacggtggct ttgcacttga tcgttccctt ggggcgggca tcggtcttga
     1321 agatgaccag ccagggcagc gtcgcggcac cgaccccggt tccgttcggc atcgtccaga
     1381 tccagttctg gtcgtcttcg acgtatccgt cccactcgac ctgggtcgga gcgtcggtgt
     1441 cgatgtacct ctgaacgaca tcgcgaatcg gcaggtcgat cttctttgag tcaggggtgc
     1501 ggaacttgac gaaactcgtt tccggaactt cctgctcctg aagggcgtcg ctgggcttcg
     1561 gccaggcggc ccgcaggcgg ctcgagtgag ccatccggtt cgtttcgccg gcagcgttcg
     1621 aattgacgtc gcagcggacc gtggtcagtt tgcgtccgcc ggaatcgatc cggaccacgc
     1681 cggccacccg gtagatgagt ccgccctcgt cattgggcgc cagcgcggaa aggctgcttc
     1741 tggccaccgc gaacgagccg ttgccgagat ccctggcggg tggtgtgctg accacctcgg
     1801 tggtatccca gtcgatttca agcgagccgc tgccgggcag ctgcgcgatc gcgtagacgg
     1861 cggccgtggc cagcaggccg accacgacca cggcgatcgc caggcgagga atgcggtcac
     1921 gcttcgcgga ctcgtcgggt gtctgttgag gggctgggtc agtcatcgga cggcggtgcg
     1981 gaggatattc gatgcggcgc ggccggcagc gcgtcgggac ggttccggcc tcaagggttg
     2041 cgcggcttta cataacccgg ttagggtggg aatgtcgagc tgaaaccggg atttggggga
     2101 tcaaatgctc atcggaaatc agacgaaggc gcggcttgtc gcaacgctcg ctggcgctct
     2161 tgtcgcactc ttcagtcttg tcgccgtcgc cagggcgatc gtcgagcagg agccgaacga
     2221 cattcgagcc gaagcaaacg gaccgatcat ggacggtgac acctg
//
LOCUS       DAOQFI010000299         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27285_length_2262_cov_2.527866, whole genome shotgun sequence.
ACCESSION   DAOQFI010000299 DAOQFI010000000
VERSION     DAOQFI010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27285_length_2262_cov_2.527866"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PLE93_05960"
     CDS             complement(<1..1140)
                     /locus_tag="PLE93_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNH86628.1"
                     /translation="MAALIAGLVLLGATTTVANAGSKQLKKAPKITSKADYTARGSVG
                     DAYVKDADPGQRLLLVNENNRIVRSGKADRFGSKIFYDVAPDRIFTVRTPTANGSQGS
                     RRFRVLKPGQNPKQSWYDAKPDLKAGLNYVTMRDGTELAVTVRLPYGKTSLSQGPFPT
                     YVEYSGYQTAAPHDALLGLVAGLPDSSLPEPLGDLLDTITGSLPGSPVPDPDLTPASS
                     TAVGSLIGPLLDFATVSVQMRGSGCSGGAFDLFGWPTTYDGYDAIETIAAQDWVKGGK
                     VSMGGISFSGITQLFVAGTRPPHLSAVSPLSTTDDVYYGTGFPGGIFNNGFAYSWITE
                     RAHDAEPAPEGGQPWARKMASTGDPQVSEPLRSEQKQHCIDNQKMR"
     gene            complement(1425..>2262)
                     /locus_tag="PLE93_05965"
     CDS             complement(1425..>2262)
                     /locus_tag="PLE93_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNH86629.1"
                     /translation="RILMDNGAAVDGDPGAIGAKWEADFNSWPIRTTRPTAFYLGKNG
                     TLSTSKSKTGSSVSYLSDPSARPAKTLGENAQGDSWLAQPPYNWQPVADGHGLGFITP
                     ALTKDVFIAGPSSVDLYLKSSKPNTDIQVTLTEVRPDGNETYIQNGWLRASHRAINSK
                     LSTAYNPVQTWLKSDQAPLKKGKYNYARIQIFPAAHVFRAGSKIRLNIQAPGGDRTIW
                     DFDTIEKGNTTNTIGLGGVIPSKLVLPVIPGEDAQGTELPGPTELRGEPNRTYEPASN
                     GG"
BASE COUNT          334 a          674 c          816 g          438 t
ORIGIN      
        1 ccgcatcttc tggttgtcga tgcagtgctg cttctgctcg ctgcgcaggg gctcggagac
       61 ctgcgggtca ccggtcgacg ccatcttgcg agcccagggc tgaccgccct cgggggccgg
      121 ttcggcgtcg tgggcccgtt cggtgatcca gctgtaggcg aagccgttgt tgaagatgcc
      181 gcccgggaaa ccggtgccgt agtagacgtc atcggtggtc gagaggggtg agaccgcgga
      241 aagatggggc ggccgcgtgc cggccacgaa gagctgggtg atccccgaga aggagatgcc
      301 gcccatgctg accttgccgc cctttaccca gtcctgggcc gcaatggttt cgatcgcgtc
      361 gtatccgtcg taggtggtcg gccagccgaa gaggtcgaag gcgccgccgg agcagccgga
      421 accgcgcatc tggacgctga ccgtggcgaa gtcgagcagc gggccgatca gtgagccgac
      481 cgcggtcgag ctggccgggg tcaggtccgg atcggggacc gggctgccgg gcaggctgcc
      541 agtaatcgtg tcgagcaggt caccgagtgg ttcgggcagg ctgctgtcag gcaggccggc
      601 gaccaggccg agcagcgcat catggggagc ggccgtctgg tagccggagt actcgacgta
      661 ggtcgggaag ggaccctgag agagggaagt cttgccgtaa ggcaggcgta cggtgaccgc
      721 gagttcggtg ccgtcgcgca tcgtcacgta gttgagaccg gccttgaggt ccggtttcgc
      781 gtcgtaccag gactgcttcg gattctgtcc gggcttgaga accctgaacc gtctcgagcc
      841 ctgcgagccg ttggcggtcg gggtgcggac cgtgaagatc cggtccgggg cgacgtcata
      901 gaagatcttg gagccgaagc ggtcggcttt gcccgagcgg acgatccggt tgttctcgtt
      961 gaccagcagc aggcgctggc ccggatcggc gtccttgaca taggcgtcgc cgacgcttcc
     1021 gcgtgccgtg taatccgcct tcgaggtgat cttcggagcc ttcttgagct gtttggagcc
     1081 cgcgttcgca acggtcgtgg tggcgccgag caggacgagt cctgcgatca gggctgccat
     1141 ggtggacaga aacacatgaa tccttcgcat cgttgcgaaa cctcccggtt gttttcggtc
     1201 gcggactccc cgcgtgcctg tttcgagcaa tggcctttca ttccccgaaa cccgatctca
     1261 taaacagcct gtttcccaag gaagtcggtt aaccaaaaag ggccgccctt ttggacggcc
     1321 ctccgccgga gccctgccat caaatatttg atggccctgg ccgggcgggt tcccgctgcc
     1381 gcccggccag ggccgggcgg cgtgcaggtg gagtgacggc ggcgtcagcc gccgttactg
     1441 gcgggttcgt aggtgcggtt gggctcgccg cggagctcgg tcggtccggg cagctcggtg
     1501 ccctgggcgt cttcgcccgg gatcaccggc aggaccagct tcgacggaat cacgccgccg
     1561 agaccgatcg tgttggtggt gttgcccttt tcgatcgtgt cgaagtccca gatcgtccgg
     1621 tccccaccgg gtgcctggat gttcaggcgg atcttcgagc cggcccggaa cacgtgggcg
     1681 gccgggaaga tctgaatccg ggcgtagttg tacttgcctt tcttcagcgg agcctgatcg
     1741 gacttcagcc aggtctgcac cgggttatag gcggttgaga gcttgctgtt gatggcccgg
     1801 tgcgaggccc gcagccagcc gttctggatg taggtctcgt tgccgtccgg gcggacctcg
     1861 gtcagggtca cctggatgtc cgtgttgggc ttgctcgact tgaggtagag atcaacgctg
     1921 gacggtccgg cgatgaagac gtccttggtc agggccggcg tgatgaagcc gagcccgtgg
     1981 ccgtcggcga ccggctgcca gttgtacggc ggctgggcca gccaggagtc accctgggcg
     2041 ttctcgccga gggtcttggc cggacgggcg ctcgggtcgg acaggtagct gaccgaagaa
     2101 ccggtctttg acttgctggt gctcagggtg ccgttcttgc ccaggtagaa cgcggtgggc
     2161 ctggtcgtcc tgatcggcca gctgttgaag tcggcctccc acttggcgcc gatcgcgccc
     2221 gggtcaccgt caaccgcggc gccgttgtcc atcaggatcc gg
//
LOCUS       DAOQFI010000300         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27332_length_2260_cov_2.444898, whole genome shotgun sequence.
ACCESSION   DAOQFI010000300 DAOQFI010000000
VERSION     DAOQFI010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27332_length_2260_cov_2.444898"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLE93_05970"
     CDS             complement(<1..694)
                     /locus_tag="PLE93_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNH86630.1"
                     /translation="MAEGDSLVRIARKLEPAMLGREIVAASAPNPRSTLRQHARRIDG
                     KRVERIETRGKHLLIHLDEGLTLHSHLGMNGGWRVDEGRGFGKPDRSAWLLLEMAGEE
                     GSVSATGGSPLATGSRTTRVAQFGGTTLRLVRTVELARDPRLATLGPDILAPEFDQVA
                     AAARLRVSPDEVGVALLDQKIVCGIGNIFKSEACFEARVDPWSPAASLAKGEEDEAEI
                     LLGAGKERAAEPL"
     gene            complement(781..>2260)
                     /locus_tag="PLE93_05975"
     CDS             complement(781..>2260)
                     /locus_tag="PLE93_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH86631.1"
                     /translation="SRYARTHGPFPTAWPKERYGADPTPALKQLEAGAALVRGEIRPG
                     GTEREWCDAEVLRRVRRASLAHLRQEVEPADGAAYARFLASWQGIDRHRPLRRDARPG
                     AGTDRLREILVPLQGVALTQSVWENDVLARRLGSYSPTWLDELCTSGEVVWIGAGAVG
                     RTDGRVALYFREDVRLAGPPPANAKLTPPEGEVHDAIRERLAAGPAFWLDLANELDHP
                     SEELHSALWDLAFGGEITNDSFAPLRAPRLRSVPTYEPGGRRFSRRRAATGPTVQGRW
                     SLTETLFRNAPAAGPKLRAQAELMLERYGILTREMALAEGIPGGFSTLYPELSNLEVL
                     GTARRGYFVEGLGGAQFALSGAVERLRALPAEENGPDAFTVLAATDPASPWGSTLPWP
                     KLESGRKAARTAGADVLARAGHPLLYVERGGKGILRLDPALEGEGLAAALATLIDEVE
                     AGRVGQLKIERFDGEPILGSAFEQLLVAAGFSRQPRRLVAPA"
BASE COUNT          316 a          777 c          834 g          333 t
ORIGIN      
        1 gcagcggctc ggcggcgcgc tccttccctg cgccgagcag gatctcggcc tcgtcttcct
       61 caccctttgc cagcgatgcg gccgggctcc agggatcaac tctcgcctcg aaacaggcct
      121 ccgacttgaa gatgttgccg atcccgcaga cgatcttctg gtcaagcagg gcgactccga
      181 cctcgtccgg actgacacgg agccgcgccg ccgcagccac ctggtcgaat tccggggcga
      241 ggatgtcggg accgagcgtc gcaagccgcg ggtcgcgggc cagctcgacc gtgcggacca
      301 ggcgcagggt cgtgccgccg aactgggcaa cccgcgtcgt tcgtgagccg gtggccaacg
      361 ggctgccacc ggtcgcgctc acactccctt cctcacctgc catttccagc agcagccagg
      421 cactgcggtc gggcttgccg aagccacggc cttcgtccac ccgccagccg ccattcatgc
      481 cgagatggga atggagggtc agcccttcgt cgagatgaat cagcagatgc ttgccgcggg
      541 tttcgatccg ctccacccgc ttgccgtcga tgcggcgggc atgctggcgc agagtggagc
      601 ggggattcgg tgccgaggcc gccacgatct cccggccgag catcgccggc tcgagcttcc
      661 gggcgatgcg aaccagcgaa tcaccttctg ccataggtca gctcgtcctc gttccgggtg
      721 acatttgggt ggttatagcg acccaaatgt cacccggaat gtgctctacg ccagttgcgt
      781 tcacgcgggg gcgaccaggc ggcggggttg gcggctgaag ccggccgcga ccagaagttg
      841 ctcgaaggcg gagccgagga tcggttcgcc gtcgaatcgc tcgatcttga gctggccgac
      901 ccggccggcc tcgacctcgt cgatcagggt ggcgagggcg gcggcgaggc cttcaccctc
      961 aagggccgga tcgagccgga ggatgccctt gccgccccgc tcgacgtaga gcagcggatg
     1021 accggcccgg gccagtacat ccgcgccggc ggtgcgggcg gccttgcggc ccgactcgag
     1081 ctttggccag ggcagggtcg aaccccaggg gctggccggg tcggtggcgg ccagcacggt
     1141 gaaggcgtcc gggccgttct cctcggccgg cagggcccgc agcctttcga ccgctccgga
     1201 gagggcgaac tgggcgccgc ctaggccttc gacgaaatag ccgcgccggg cggtacccag
     1261 cacctcgagg ttggagagtt ccgggtagag ggtggagaag ccgccgggta tgccctcggc
     1321 cagggccatc tcccgggtca ggatgccgta gcgctccagc atcagctcgg cctgggcccg
     1381 cagcttcggg ccggcagcgg gggcgttgcg gaacagggtc tcggtaagtg accagcggcc
     1441 ctggacggtc gggccggtgg cggcacggcg acgcgagaag cggcggccgc ccggctcgta
     1501 ggtcgggacc gaccggagcc gcggcgcccg gagcggcgcg aaggagtcgt tggtgatctc
     1561 gccaccgaag gcgaggtccc agagggccga gtgcagttcc tcggaagggt gatccagctc
     1621 gttggcgagg tcgagccaga aggccgggcc ggcggcgagc cgctcgcgga tcgcgtcatg
     1681 gacttcgccc tccggcgggg tcagcttcgc gttggccggt ggcgggccgg ccagccgcac
     1741 gtcctcccgg aagtagagcg cgacccgtcc gtcggtgcgg ccgaccgccc cggctccgat
     1801 ccagaccacc tcgccgctgg tgcagagctc gtccagccag gtcgggctgt aggagccgag
     1861 ccggcgggcc agcacgtcgt tttcccatac cgactgggtc agggccacgc cctgcagcgg
     1921 aaccaggatc tcgcgcagcc ggtcggttcc ggccccgggg cgggcgtcac ggcggagcgg
     1981 gcggtggcgg tcgattccct gccatgaggc gaggaagcgg gcgtaggcgg cgccgtcggc
     2041 cggttcgacc tcctgcctga ggtgagcgag gctggcccgc cggacccggc gcagcacttc
     2101 cgcatcgcac cattcgcgct cggtgccgcc cgggcggatc tcgccgcgaa ccagggcggc
     2161 gcccgcttcg agctgcttga gggcaggggt cggatccgcc ccgtagcgct ccttcggcca
     2221 ggcggtcggg aacgggccat gggtcctcgc gtagcggctc
//
LOCUS       DAOQFI010000301         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27387_length_2257_cov_2.693460, whole genome shotgun sequence.
ACCESSION   DAOQFI010000301 DAOQFI010000000
VERSION     DAOQFI010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27387_length_2257_cov_2.693460"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="PLE93_05980"
     CDS             complement(<1..1660)
                     /locus_tag="PLE93_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNH86632.1"
                     /translation="MSDSSQTTSADEKRLAELGYAQTLDRAWSSFSNFAISFTIISVL
                     AGCFTTYQVAWNAGGPIAISWGWPIICGLILLVAVSMSELVSKYPTAGGIYYWASDLG
                     GKTWGWFTGWFNLIGLVGVTASVVYGSAAFLNTVLSIYGVDLGFINFADSEHILSETF
                     VLFVIILTIHSLINIYSSPLVAQFNNVSVWWHVIGVAVIIGLLIFVPDKHASVDFVFT
                     QTFNNTGLFGGTTVGAAFWFYVLPLGFLLTMYTQTGYDASAHISEETKGAAMGAAKGV
                     WRSVFWAGLIGWFVLLAITFAASSGLVDTINDGDAAPFGAGTALSILDLALSSWAAKM
                     VLIIATVGQLFCGMACLTSSSRMCYAFSRDRAVPGHKLWTRLNHHRVPHLSVISMAAF
                     ALLITVPALIGDDAIPFAFTAVVSIAVIGLYIAYMIPIYLRWRMGDAFEPGPWTLGRK
                     YKWINPIAVVWVIICVITFSVPTAAISVPWDETFDIKYLNYAPITVLIVIVGVGLWWM
                     LSAKNHFEGPIRQVATDDTGRVIDEEALAEAHLSDQPEPTQTGDS"
     gene            complement(1749..>2257)
                     /locus_tag="PLE93_05985"
     CDS             complement(1749..>2257)
                     /locus_tag="PLE93_05985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH86633.1"
                     /translation="RLKRERRRAARAPRTRRGDAVDPFGFHIYGFMFPLRGPHTYGDG
                     IGAPRSGHTHQGQDVLSQCGLPLRAARAGVVYYNDYQAGGAGNYIVIDTVGTGGKSHV
                     YMHMVRPSPLKVGTHVKTGQLIGRVGTTGASSACHLHFEIWSGPGWYQGGTFLDPTPA
                     LRVWDRYS"
BASE COUNT          461 a          653 c          765 g          378 t
ORIGIN      
        1 cggaatcccc ggtctgtgtg ggttcaggtt gatcactcaa atgcgcctcg gcgagcgcct
       61 cctcgtcgat cacccggccc gtgtcatccg tcgcaacctg gcggatcggg ccctcgaaat
      121 ggttctttgc cgagagcatc caccaaagac cgactccgac gatcacgatg agcacggtga
      181 tcggggcgta gttcaggtat ttgatgtcaa acgtctcatc ccacggaacc gaaatcgccg
      241 cggtgggcac gctgaaggtg atcacgcaga tgatcaccca gacgaccgcg atcgggttga
      301 tccacttgta cttcctgccg agtgtccacg ggccgggttc gaaggcatcg cccatacgcc
      361 accgcaggta gatcggaatc atgtaggcga tgtagagacc gattacggcg atcgatacca
      421 ccgcggtgaa ggcgaacgga atggcgtcgt caccgatcag tgccggaacc gtgatgagca
      481 gtgcgaacgc ggccatcgag atcactgaca ggtggggcac ccggtggtgg ttgagccggg
      541 tccacagttt gtgcccggga acggcacggt cacgcgagaa ggcgtaacac atgcgggaag
      601 agctggtcag gcaggccatg ccgcagaaaa gctggccgac cgtggcgatg atcagcacca
      661 tcttggctgc ccaggaagat agggccagat cgagaatcga cagcgcggtg ccggctccaa
      721 acggtgccgc gtccccgtcg ttgatcgtgt caacgaggcc cgatgatgcg gcgaaggtaa
      781 tcgcgagcag tacgaaccaa ccgatcagtc cggcccagaa caccgatcgc cagacacctt
      841 tggcggcgcc catcgcggcg cccttcgtct cctcactgat gtgcgcggag gcgtcgtacc
      901 cggtctgtgt gtacatggtc aggaggaagc cgagtggcag gacatagaac cagaaggcgg
      961 cgccgaccgt ggtgcccccg aagagtccgg tgttgttgaa ggtctgggtg aagacgaaat
     1021 cgacgctggc gtgtttgtcc ggcacgaaga tcagcagccc gatgatcacc gcgacgccga
     1081 tcacgtgcca ccagacagag acgttgttga actgcgccac gaggggggac gaatagatgt
     1141 tgatcaggga atggatcgtg aggatgatca cgaacagcac aaacgtctcg gacaggatgt
     1201 gttcgctgtc ggcgaagttg atgaatccga gatcgacgcc gtagatgctg agaacggtgt
     1261 tgaggaaagc cgcggaaccg tagacgaccg aggcggttac tccgaccagt ccgatcaggt
     1321 tgaaccagcc ggtgaaccag ccccaggtct tgccgccaag gtcggaggcc cagtaataga
     1381 tgccgccggc cgtggggtac ttggaaacga gttcggacat cgaaacggca accagcagga
     1441 tcaggccgca gatgatcggc cacccccacg agattgcgat tgggccgccg gcgttccagg
     1501 cgacctggta ggtggtgaag cacccggcca gaaccgagat gatcgtgaac gagatcgcga
     1561 agttggagaa cgaactccac gcgcgatcga gtgtttgggc gtagccgagt tcggctagac
     1621 gcttctcatc ggcactggtt gtctgggacg agtcagacat gcagacctct ctcctcgggg
     1681 atggaatgac cgcagcctag ggccgcgcgt cgtcaccgtc aagccggtcg ttcagccggg
     1741 cccggttttc aggagtagcg gtcccagacc cggagtgccg gggtgggatc gaggaaggtg
     1801 ccgccctgat accagccagg gccggaccag atctcgaagt ggaggtggca ggcgctgctg
     1861 gctccggtcg taccgacgcg tccgatcagc tgtccggtct tcacgtgcgt gccgaccttg
     1921 agcggcgagg gacggaccat gtgcatgtag acgtggctct tgccgccggt gccgaccgtg
     1981 tcgatcacga tgtagttgcc ggctccaccc gcctggtagt cgttgtaata gacgacaccg
     2041 gcacgggcgg cccgcagcgg caatccgcac tggctcagta cgtcctggcc ctgatgggtg
     2101 tgaccggagc ggggagcgcc gatcccgtcg ccgtatgtat gcgggccgcg gagcgggaac
     2161 atgaaaccgt agatgtggaa gccgaagggg tcgaccgcat cgccgcggcg ggtcctcggg
     2221 gcacgggcgg cccggcgacg ctcgcgcttc aggcggg
//
LOCUS       DAOQFI010000302         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27583_length_2248_cov_3.559052, whole genome shotgun sequence.
ACCESSION   DAOQFI010000302 DAOQFI010000000
VERSION     DAOQFI010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27583_length_2248_cov_3.559052"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLE93_05990"
     CDS             complement(<1..258)
                     /locus_tag="PLE93_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNH86634.1"
                     /translation="MIATIAKAFSVPEIRRKLLFAAAMLALYRLGAYIPAPGIDVDAV
                     KELESQLTGSNILGFLNLFSGGSLSRLSLFALGIMPYITASI"
     gene            complement(258..725)
                     /gene="rplO"
                     /locus_tag="PLE93_05995"
     CDS             complement(258..725)
                     /gene="rplO"
                     /locus_tag="PLE93_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNH86635.1"
                     /translation="MGLHNLSPKPGSRRPKKRIGRGPGSGTGKTSGRGHGGQGQRSGN
                     KRKVNLEGGQTPIHMRMRKLRGPHMKKSMPFENFRTATQPVNLGDLEDRFDAGAEVTP
                     ETLKAAGLATRKDPVKILARGEISKSLTVKAHGFSAAAKEKIEAAGGSCELID"
     gene            complement(758..937)
                     /gene="rpmD"
                     /locus_tag="PLE93_06000"
     CDS             complement(758..937)
                     /gene="rpmD"
                     /locus_tag="PLE93_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNH86636.1"
                     /translation="MAKLAIKQIRSDINTNPSQRGTLAALGLGKIGRKVERDDSPQLQ
                     GQLKVVSHLVEVEEA"
     gene            complement(1399..1884)
                     /gene="rpsE"
                     /locus_tag="PLE93_06005"
                     /pseudo
     CDS             complement(1399..1884)
                     /gene="rpsE"
                     /locus_tag="PLE93_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002063426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(1941..>2248)
                     /gene="rplR"
                     /locus_tag="PLE93_06010"
     CDS             complement(1941..>2248)
                     /gene="rplR"
                     /locus_tag="PLE93_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNH86637.1"
                     /translation="VRARVSGTAQRPRLSVYRSNRGVFAQLIDDVDGRTLAAANWTEP
                     ELKKLSGTDQAKKAGELLAERAKKAGVETCVFDRSGYRYHGRVKALAEGAREGGLEF"
BASE COUNT          322 a          740 c          719 g          467 t
ORIGIN      
        1 gatcgaggcc gtgatgtagg gcatgatgcc cagcgcgaaa agcgaaagcc tcgagagcga
       61 gccgcccgaa aacaggttca ggaagccgag gatgttcgat ccggtcagct ggctttcgag
      121 ttccttgacg gcatcgacgt cgatgccggg ggccggaatg taggcaccga gccggtacag
      181 ggccagcatg gccgctgcga agagaagctt ccggcgaatc tccgggacgg agaatgcttt
      241 cgcaatcgtt gcgatcatca gtcgatcagc tcgcagcttc cgccggcggc ttcaatcttc
      301 tccttggccg cggccgaaaa gccgtgggcc ttgacggtca ggctctttga aatctcgccg
      361 cgggcgagga tcttgaccgg atccttgcgg gtcgccagac cggcagcctt gagcgtttcc
      421 ggggtgactt ccgcaccggc gtcgaagcga tcttcgagat cgccaaggtt gaccggctgg
      481 gtcgcggtcc ggaaattttc gaacggcatc gacttcttca tgtgcgggcc gcgcagcttg
      541 cgcatccgca tgtgaatcgg ggtctggcca ccttcgaggt tgaccttccg cttgttgccc
      601 gagcgctggc cctggccgcc gtgtccgcgg ccggaggtct tgccggtgcc cgaaccgggt
      661 ccgcggccga tccgcttctt cggacggcgc gagccgggct tggggctcag attgtgaagg
      721 ccgatttctt cttcgcggct ttcctgcttg tcagccatca ggcctcctcc acctcgacca
      781 ggtgggaaac caccttgagc tggccctgga gctgggggga atcatcccgc tcgaccttgc
      841 ggccgatctt gcccaggccc agagcggcaa gcgtgccacg ctggctcggg ttcgtgttga
      901 tatctgaacg aatctgcttg atcgcgagct tggccatcag gcgttgtcct cttcagcctt
      961 ctcggcgtcc tcggcctttt cttcgctcgc ctgtacttct tcggcggctt caaccacggt
     1021 ctcggcctgc gcttcatcgg cagcgacctc ggcctctacc tcagaggcag cgggaccggt
     1081 cggctcgggg gccggggcgt cggtgccggc tccatcagtt gcctcttcgg ccggcacctt
     1141 cgcgtcagca gtgtcctcgg cagccttctc ctcgaccgcg gcgatgtcag ccggggtttc
     1201 ggtggtctcg gcggcctctg cagccggagc ttcgtcggct tcggtcgcgg cgtcctcgac
     1261 gagggcttcc tcggcgggga gttcggcggc agcagtttcc tgatcgctgg cagcctcggg
     1321 agcggaaccg ttgccgttgc cgttggcagc ggcttcctcg gtggcttcct cggccttggc
     1381 gtccttgtcg atgcccagga cctcgctgat cgtgagacca cgcagttcgg cgacctcttc
     1441 cggccggcgc agacggatga tgccgtccat ggtcgccttg accaggttga tcgggttctg
     1501 ggtgccgacg gacttggtga ggatgtcgcg cacgccgccc agttcgagca cggcacggac
     1561 gccgccgccg gcgataacgc cggttcccgg gctggccggg cgcagcacca cgtgaccggc
     1621 gccgtagcgg ccgatgatct tgtgggtgat cgtgtccccg tacttgggga tccgaaccag
     1681 gttcttgcgg gcgttctcga cggccttctg gatcgcgagc ggaacctcgc gggccttgcc
     1741 gtaaccgatg ccgactaccg actcttcgtc acctactgca acgagggcag tgaacgagaa
     1801 gcgacgacca cccttgacca ccttggccac acggttgatc tcgatcacac gctcctgcag
     1861 gtcgagcccc ttcgggctga ccatctcaat gcgggctgta tccattccct tcatttcagc
     1921 tcttcaccgt accggagttc ctagaactcc agtccaccct ctctcgcacc ttcggccagg
     1981 gccttgaccc tgccgtggta gcggtaaccg cttcggtcga agacacaagt ctcgactcct
     2041 gccttctttg cacgttctgc cagcagttcg ccggccttct tcgcctgatc ggtgccgctg
     2101 agcttcttga gctcgggctc ggtccagttc gcggcagcca gcgtcctgcc atcgacatcg
     2161 tcgatgagct gggcgaaaac gccacggttg gaccggtaca cggacaggcg gggacgctgg
     2221 gcggttccgg acaccctggc ccggaccc
//
LOCUS       DAOQFI010000303         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27606_length_2247_cov_2.812956, whole genome shotgun sequence.
ACCESSION   DAOQFI010000303 DAOQFI010000000
VERSION     DAOQFI010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27606_length_2247_cov_2.812956"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLE93_06015"
     CDS             complement(<1..349)
                     /locus_tag="PLE93_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH86638.1"
                     /translation="MPTARIINAANGMTAEDLVRYALEHNGELLAARKLIAEAQGRLR
                     QAGLKANPMLETGGKQAMTTLDNNQMFSVELPLELGGRRQSRMLVGQREVELREAEVR
                     DFERKLAAEVRLKY"
     gene            342..899
                     /locus_tag="PLE93_06020"
     CDS             342..899
                     /locus_tag="PLE93_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86639.1"
                     /translation="MGIEDTALSIFQTLTAAIPLAAIAAQVDRHLAGTPVATQEDLAP
                     ARDVMAAVREADDQSEGDSGVAVCLFGSEYSSAAGASAFFADSRLKLKQGAALVNLVR
                     GARASGPEVTGSEGDLMPTRMNPELAAGSPLKPKMVVLRNQTAATIARRRGLRATTVV
                     GRGEAGVDVLLDASEGALPDEEEKE"
     gene            896..>2247
                     /locus_tag="PLE93_06025"
     CDS             896..>2247
                     /locus_tag="PLE93_06025"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNH86640.1"
                     /translation="MSPEPLWSPDPEAIESSQLVAWAHWLKDRDLGPGTAEYADLWQW
                     STDQPEEFWRSIWDYFEVLHDGEPAAVLSGHEMPGARWFEGTSLNYAEHIFRDRDPEA
                     AAVFAASEVRALETVTWGELRSRVADCAAGLKALGVGPGDRVAGYLPNSHETIVAFLA
                     VASLGAVWSSCSPDFGAGAVTDRLAQIEPRVLLAVDGYNYGGKGFDRRGVVSDLVAGM
                     PTVEQVVILPYLEPDPDLTPLPGAITWNELLSKDPGAELTFTRVPFDSPLWILYSSGT
                     TGLPKPIVQSQGGILLEHLKKMHLHLDAGEGDRIFWFTTTGWMMWNFLVSCLLTDAAI
                     VLYDGNPGHPDMSALWQLASDAGVTCFGTSASYISACMKDGVEPGADGRDLSKLRSVG
                     STGSPLAPEGFDWVYEHIGKDTWLFSTSGGSDLCTAFVGGVPTLPVYRGELQARALGA
                     A"
BASE COUNT          346 a          766 c          723 g          412 t
ORIGIN      
        1 cgtatttcag ccgcacttcc gccgccaact tgcgctcgaa atcgcggacc tcggcttcgc
       61 gcaattcgac ttcgcgctgg ccgacgagca tgcgcgattg ccgccgtcca cccagttcca
      121 gcggcaattc gacgctaaac atctggttgt tatccaaggt ggtcatggct tgtttgccgc
      181 ccgtttccag catcgggttg gctttcaaac ccgcttggcg caaacgccct tgggcttcag
      241 caatcagctt gcgcgccgcc agcaactcgc cgttgtgttc tagggcatag cgcaccaaat
      301 cctcagcggt catgccgttc gccgcgttga tgatgcgggc cgtgggcatc gaggacaccg
      361 ccctcagcat cttccagacc ctgaccgcgg cgatcccgct cgccgcaatc gccgctcagg
      421 tcgaccgcca cctggccggg accccggtcg ccacgcagga ggatctcgcc ccggcccgcg
      481 acgtgatggc cgctgtccgg gaagccgacg accagtcgga aggcgattcg ggcgtggcgg
      541 tctgcctgtt cggctcggag tattcgtcag ccgccggcgc ctccgccttc ttcgccgaca
      601 gccggctgaa gctcaagcag ggagcggctt tggtcaatct ggtgcggggc gcccgtgcct
      661 cggggcccga ggtgaccggc agcgaagggg atttgatgcc gacccgcatg aatccggaac
      721 tggcggccgg cagtccgctg aaaccgaaga tggtcgttct gcggaaccag accgcagcca
      781 cgattgcccg gcggcggggc ctgcgcgcga ccacggtggt tggccggggc gaagccggag
      841 tcgacgtcct gctcgacgct tcggaaggcg ccctgcccga cgaggaggaa aaggaatgag
      901 tcccgaaccg ctctggtcgc cggatcctga agcgatcgag agttcgcagc tcgtcgcctg
      961 ggcccactgg ctgaaggatc gtgacctggg acccggaacc gcggagtacg ccgatctctg
     1021 gcaatggtca accgaccagc ccgaggaatt ctggcgttcg atctgggatt acttcgaggt
     1081 tctccatgac ggcgaaccgg ccgcggtcct ctccggccac gagatgcccg gcgccagatg
     1141 gttcgagggc accagtctca actacgccga acacatcttc cgcgaccgcg atccggaagc
     1201 ggcagccgtc tttgccgcgt cggaggtgcg ggcgctcgaa acagtgacct ggggtgagtt
     1261 gcgaagccgg gtggccgact gcgcggcggg actgaaggcg ctgggcgtcg ggcccggcga
     1321 ccgggttgcc ggctaccttc cgaattcgca cgagacgatc gttgccttcc tcgccgttgc
     1381 cagcctgggc gcggtctggt cgagctgctc cccggatttc ggtgccgggg ccgtgaccga
     1441 ccgtctggcc cagatcgagc ccagagtcct gctcgcggtc gacggctaca actacggcgg
     1501 caagggattt gaccgtcgcg gcgtggtttc cgatctggtt gccggtatgc ccacggtcga
     1561 gcaggtcgtg atcctgccct acctggaacc ggatcccgac ctgaccccgc tccctggcgc
     1621 cattacctgg aacgaactgc tttcaaagga tcccggggcc gagttgacgt ttacccgggt
     1681 gccattcgac tcccccctgt ggatcctcta ctcctccggc accaccgggc tgccgaagcc
     1741 gatcgtccag agccagggcg gcatcctgct cgagcacctg aagaagatgc acctccacct
     1801 tgatgccggt gagggtgacc ggatcttctg gttcacgacc accggctgga tgatgtggaa
     1861 ctttctcgtc tcctgtctgc tcaccgacgc ggcaatcgtg ctttatgacg gcaaccccgg
     1921 tcaccccgac atgtcagctc tctggcagct cgcttcggat gccggggtga cctgcttcgg
     1981 cacctccgcc tcctacatct cggcctgcat gaaggacggg gtcgagcccg gcgctgacgg
     2041 ccgtgacctc tcaaagctcc gctcggtcgg ctcgaccggc tcgccgctcg cccccgaggg
     2101 cttcgactgg gtgtacgagc acatcggcaa ggacacctgg ctgttttcga cctcgggtgg
     2161 ttccgacctc tgcacggcct tcgtcggtgg cgttcccacc cttccggtct accggggcga
     2221 gctgcaggcc cgggcgctcg gggcggc
//
LOCUS       DAOQFI010000304         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27711_length_2243_cov_3.424132, whole genome shotgun sequence.
ACCESSION   DAOQFI010000304 DAOQFI010000000
VERSION     DAOQFI010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27711_length_2243_cov_3.424132"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1170)
                     /locus_tag="PLE93_06030"
     CDS             complement(154..1170)
                     /locus_tag="PLE93_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNH86641.1"
                     /translation="MPNLPRLRRARESELLSPDSVEPEVLAPRVEEITSGGLRWINIE
                     SPGGEERAWLEENFDFHALDIEDVLSHNQRPKIDEYPDYLFLVVHVPVFDRKAKRLVA
                     GEIDIFVGPDFMVTLPNRGIPPVEYLFERCRADDTLRHKLFARGSGYLMYRLVDDSFD
                     YCFPMLRKIGNKLDALEEEIFEGRSEDVVRDLSNAKQEIINFRKVIRPQRPVLRDLEN
                     VKSRYLASDLDLEIYFDDIVDAHERIWDMLENYKEVVVALEETNESIISHKVNDILRV
                     LTSISVIVLPLTLIASIFGMNVGLPGGGDPATSTDFHFFVVVGIMLVILVGMVAYFRK
                     RDWL"
     regulatory      1289..1402
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1486..>2243
                     /gene="thrC"
                     /locus_tag="PLE93_06035"
     CDS             1486..>2243
                     /gene="thrC"
                     /locus_tag="PLE93_06035"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNH86642.1"
                     /translation="MAVKNLKCKECSTEYELGANFFCSECFGPLEVKYDFSDLNPEET
                     RRRIQAGSRGIWRYSDFLPFQGKAGDPLEPGMTPLIKADRLSERLGTGAEIFIKNDAA
                     NPTHSFKDRVVAVAIAKAKELGFETVACASTGNLANAVAAHAAAAGLDSYVFVPSNLE
                     EQKLLATDIYGTNLVGVKGNYDDVNRLCTQLADEKPWAFVNVNLRPYYAEGSKTLAYE
                     TVEQLGWNLPDRVVAPIASGSLFTKISRGFQEWK"
BASE COUNT          462 a          667 c          699 g          415 t
ORIGIN      
        1 tcgatggtat agtccaaggc cgctgcccct acctctgcca tcggcgcccc atcctattgg
       61 tccaacgcga gtcggagtcc gatcatctat tcgctttctc tgtgatcgga cgggtggaac
      121 ttggaggcgg ggcgaagctc gccgactact tattcagagc cagtctcgct tgcggaagta
      181 ggcgaccatg ccgaccagga tcaccagcat gatccccacc accacgaaga agtggaaatc
      241 ggtactggtg gccgggtcgc cgccaccggg caggcctacg ttcatgccga agatcgaggc
      301 gatcagggtc aagggcagga cgatcaccga gatcgaggtg agcacccgca ggatgtcatt
      361 gaccttgtgg gagatgatcg attcgttggt ctcctcgagc gcgaccacga cctccttata
      421 gttctcgagc atgtcccaga tccgttcgtg ggcgtcgacg atgtcgtcga agtagatctc
      481 gaggtcgaga tcactggcca ggtagcggga cttgacgttc tcgaggtcac gcagaaccgg
      541 tcgctgcggc cggatcacct tgcggaagtt gatgatctcc tgcttggcgt tcgagaggtc
      601 gcgaaccaca tcctcggagc ggccctcgaa aatctcttcc tcgagcgcat cgagtttgtt
      661 gccgatcttt cgcagcatcg ggaagcagta atcgaagctg tcatcgacca ggcggtacat
      721 caggtaaccg gaaccgcggg cgaagagttt atggcggagg gtgtcatcgg cccggcagcg
      781 ctcgaagagg tactcgaccg gcgggatccc gcggttcggc agggtgacca tgaaatcggg
      841 tccgacgaag atgtcgattt cgccggcgac caggcgcttg gccttgcggt cgaaaaccgg
      901 tacatggacg acgaggaaga ggtagtccgg gtattcgtcg atctttggtc gctggttatg
      961 cgaaagcacg tcctcgatgt cgagggcgtg gaaatcgaag ttctcctcca gccaggcgcg
     1021 ttcctccccg ccagggcttt cgatgttgat ccagcgcaaa ccgcccgagg tgatctcctc
     1081 gacccgcggg gcgaggacct cgggttcgac tgaatccggg ctcagcagct cagattcgcg
     1141 cgcacggcga agacgaggga ggttcggcat cgccgcctcc attcatgtca attcgcttcg
     1201 cgggcatgaa acaaccctag accagcagtc aaacccccgc gtaaacggga tgtttatgca
     1261 tgctggtaac ctgtcgccag acataagcgc cacatccaga ggggtggagg gacatggccc
     1321 tacgaaaccc cggcaaccag cggaagcgag agctttcgag aaggtgccaa atccagcggc
     1381 tcaagtagcc gggagatgtg gggagaagtc cgatccggcc ttcgccccac gggcgaaggt
     1441 tttttgtttc cccggcaggg gaaagaacga aaggtaagag atcggatggc agtaaagaac
     1501 ctcaaatgca aggaatgttc gaccgagtat gagctcggtg cgaacttctt ctgctccgaa
     1561 tgtttcgggc cgctggaagt caagtacgac ttctcggacc tgaacccgga agagacccga
     1621 aggaggatcc aggcgggatc gcgcggaatc tggcgctatt cggacttcct gcccttccag
     1681 ggcaaggccg gcgacccgct cgagccgggc atgactccgc tgatcaaggc ggaccgcctc
     1741 tcggagcgcc tgggaaccgg ggcagagatc ttcatcaaga acgatgccgc caacccgacc
     1801 cattccttca aggaccgggt cgtcgccgtg gcgatcgcca aggcgaagga gctcggcttc
     1861 gagaccgtcg cctgcgcctc gaccggcaac ctggccaacg cggttgcggc tcatgccgcc
     1921 gcggccggac tcgattccta tgtctttgtt ccgtccaacc tggaagagca gaagctgctg
     1981 gcgaccgaca tctacggcac caacctggtc ggcgtcaagg gcaactacga cgatgtgaac
     2041 cggctctgca cccagctcgc ggacgagaag ccctgggcct tcgtcaacgt gaacctgcgc
     2101 ccgtactacg ccgagggctc gaagaccctt gcctacgaga cggtcgaaca gctcggctgg
     2161 aacctgcccg accgtgtggt cgctccgatc gcgtcgggtt cgcttttcac caagatctcc
     2221 cgcggcttcc aggagtggaa gga
//
LOCUS       DAOQFI010000305         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27754_length_2242_cov_2.519433, whole genome shotgun sequence.
ACCESSION   DAOQFI010000305 DAOQFI010000000
VERSION     DAOQFI010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27754_length_2242_cov_2.519433"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PLE93_06040"
     CDS             <1..1188
                     /locus_tag="PLE93_06040"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNH86643.1"
                     /translation="GEVAAPPYDVISGPERSSMLDTSPFNVVEIDLPESTGGDPYEHA
                     SGTLEEWILSGILKQDREPSIWALTQEFTGPDGSMKIRNAILARVRVEDYGPGRIRPH
                     ERTQPGPKQDRLDLTRATRYNLSPIFSLTTRDAWPVVAPAIEGEEAWAEVLGGDGTLN
                     RVWKIEDPEIHRAVSQELDQAELLIADGHHRYETARAYRDEVGGEGAHCYTLMALTGL
                     DDPGLTVFPTHRLLTGLKDPDLVDRLKRGVEGAFESIAEGEIDPSGVEGTGCFGLALS
                     DGSRQLLRLRDPASLNALFPDESPAFRDLDAAILEKVVLGGVLGMTETDVEAKRGLAY
                     AKTIGEATAAVDSGEAQAAFILRATPIGQVRAVAEAGETMPPKSTYFFPKIPTGIVFN
                     PLS"
     gene            1325..>2242
                     /locus_tag="PLE93_06045"
     CDS             1325..>2242
                     /locus_tag="PLE93_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86644.1"
                     /translation="MKTKALVPIALLLALALLPGAAGAADTYVAPNGSGNACTQASPC
                     SILTGGNEADADSAIILAPGDYGSAVDPIDDRIYTFAPNANYVGRNDVRLFVDASSST
                     ASVTIYSGQKFLGYGTRIISFDEVGVGIYGSARAERIDVRTSIDGSTACRFGNEGMET
                     GGQLTNSLCVADGFGSKGIELAATRNEESAMVMGSTGVSVGNGGAGITASNSGITGGG
                     TGFSRLNVFLSIARAEKGYDLDGGFDSDDNEACISARHTSALSLRPNICGIAEDNPIR
                     ELPGFIDPVSDFHLAAGSPLIDAVKNFSYP"
BASE COUNT          417 a          766 c          695 g          364 t
ORIGIN      
        1 ggcgaagtcg cggcgccgcc gtatgacgtg atcagcggcc cggaacggtc ctcaatgctc
       61 gacacttcac cgttcaacgt ggtcgagatc gacctgccgg agtcgaccgg tggcgacccc
      121 tacgaacacg catccggaac ccttgaagag tggatcctca gcggcatcct caaacaggat
      181 cgtgaaccct cgatctgggc cctgacgcag gagttcaccg ggcccgacgg ttcgatgaag
      241 atccgcaacg cgatcctcgc ccgggtgcgg gtcgaggatt acgggcccgg ccggatccgg
      301 ccccacgagc ggacccagcc cggcccgaag caggaccggc tcgacctgac ccgggccacc
      361 cggtacaacc tctcgccgat cttctcgctg accacccgtg acgcctggcc ggtggtcgcc
      421 ccggcgatcg aaggcgagga agcctgggcc gaggtgctcg gcggggacgg caccctgaac
      481 cgggtctgga agatcgaaga cccggagatc caccgcgcgg tcagccagga acttgatcag
      541 gccgaactgc tgatcgccga cggccatcac cgctacgaga ccgcccgcgc ctaccgggat
      601 gaggtcggtg gcgagggcgc tcactgctac accctgatgg ccctgaccgg actcgatgac
      661 ccgggcctga ccgtgttccc cacccatcgc ctcctgaccg gcctcaagga ccccgacctg
      721 gtcgatcgcc tcaagagagg cgttgaaggg gcgttcgaat cgatcgctga aggagaaatc
      781 gacccgtccg gagtcgaagg aaccggatgc ttcggccttg ccctttcgga cggatcgagg
      841 cagctgcttc ggctccggga tcccgcctcc ctcaatgccc tgttcccgga tgagtcgcca
      901 gccttccggg atctggatgc ggcgatcctc gaaaaggtcg tcctgggcgg cgtcctcggg
      961 atgaccgaga ccgatgtcga agccaaacgg gggctcgcct acgcaaagac gatcggggag
     1021 gcaaccgcgg cggtcgattc gggagaggcc caggccgcct tcatcctccg tgccacaccg
     1081 atcgggcagg tccgggcggt cgccgaggcg ggagaaacca tgccaccgaa gtcgacctac
     1141 ttcttcccca agatcccgac cggcatcgtt ttcaaccccc tgagctgacc ggattcccgg
     1201 ctgtggatcg attaaccccg tatgccgggt taatccgtac acaggtgaag ccaagcggcc
     1261 gggatggccg gtttcagccc ttgggtcgta gccaggtccc ggcttcggcc ggatgctgaa
     1321 ctcgatgaag accaaagcgc tcgtcccgat tgccctgctc ctcgccctcg ccctgctccc
     1381 cggagcggcc ggagcggccg acacctatgt tgcgccgaac ggctccggca acgcctgcac
     1441 ccaggcgtcg ccctgctcga tcctcaccgg cgggaatgaa gccgacgcgg actcggcgat
     1501 catcctggct cctggcgatt acggatccgc cgtagacccg atcgacgacc ggatctacac
     1561 attcgcgccg aacgccaact atgtcggccg aaacgatgtt cggttgttcg tcgatgcctc
     1621 gtccagcacc gcctcggtga ccatctactc cggccagaag ttcctcggct acgggaccag
     1681 gatcatcagc ttcgatgagg tcggggtcgg catctacggt tcggcccgcg ccgaaagaat
     1741 cgatgtcagg acgagcatcg acggcagcac tgcctgccgc ttcggcaacg aagggatgga
     1801 gaccggaggc cagctcacca actcgctttg cgttgccgac ggattcgggt ccaaaggcat
     1861 cgagctggcc gcgacccgga atgaagaatc agcgatggtc atgggttcga ccggagtttc
     1921 ggtgggcaac ggcggagccg gaatcaccgc ttcgaacagc ggcattaccg gcggcggaac
     1981 cggcttcagc cggctcaacg tcttcctctc gatcgcccgg gccgagaagg gatacgacct
     2041 cgatggcgga ttcgactccg atgacaacga agcctgcatc tcggcccgac acacatccgc
     2101 gctcagcctg cggccgaata tctgcgggat cgcggaagac aacccgatcc gggaactgcc
     2161 cggcttcatc gatccggtct ccgacttcca tctcgccgcg ggttcacccc tgatcgatgc
     2221 cgtgaagaac ttcagttacc cg
//
LOCUS       DAOQFI010000306         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27817_length_2239_cov_4.116300, whole genome shotgun sequence.
ACCESSION   DAOQFI010000306 DAOQFI010000000
VERSION     DAOQFI010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27817_length_2239_cov_4.116300"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLE93_06050"
     CDS             complement(<1..590)
                     /locus_tag="PLE93_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH86645.1"
                     /translation="MNPVKSAAQAVANKAFEIRTLHKAGVVGGLGPAALPVAASALIR
                     GGTTPATAIRVAAVKNGDRVYLEDERGSLTFAEVHRRSNALAHAFAGLGIGPGDGLAV
                     MCRNHRGFVETNLAGWKLGANVILLNTMFSGPQLSDVIEREGAKAVVLDQEFEELLSG
                     VPGGIGRVCGFEDEPGTSGLPSLEDLIAGNDDGELP"
     gene            complement(614..1075)
                     /gene="ispF"
                     /locus_tag="PLE93_06055"
     CDS             complement(614..1075)
                     /gene="ispF"
                     /locus_tag="PLE93_06055"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNH86646.1"
                     /translation="MAISVGIGYDSHRFADDRRLILGGVEVPHEMGLSGHSDADVLTH
                     AIIDAVLGAAGLGDIGEVFPDTDPAWEGADSISLLSTVIGMLRGPITNVDATLICEQP
                     KLGPHRPAMVARLSEITGSRVSVKATTNEQMGWIGRGEGIACMAVAAVNLD"
     gene            complement(1116..1811)
                     /locus_tag="PLE93_06060"
     CDS             complement(1116..1811)
                     /locus_tag="PLE93_06060"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNH86647.1"
                     /translation="MLVSLSLLLVWDGSAAASAARPEAFAEKVALPAVGEALAPRIAV
                     RARPISKAPVRAVMKAFRFDYRPTVFFAIDRRETGQGEWLKVRIPGRPNGRTGWVRGS
                     RIRLLRSAGPFRIVIDRSQRRLRLYRHKRMVMKTKVAVGTPDAPTPLGHFYLTAGFVP
                     GDDFLGPWAFETSAYAAITDWPRGGIVGLHGTSETWSVGLRASHGCLRVYNKVILALK
                     KRVRPGTPLRIRA"
     gene            complement(1841..>2239)
                     /locus_tag="PLE93_06065"
     CDS             complement(1841..>2239)
                     /locus_tag="PLE93_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86648.1"
                     /translation="KVHFTARATSTEARWTVSVPQSHQKAVLAPKRQETCRAMNVTPR
                     TIDINKTTVLTVRLRTPEGRPAIGITVVARGQGTGDSARTNGKGMARLRVRPNGQGYL
                     VVTAPNATRCRFRIGASDLDAAGAGNQLTG"
BASE COUNT          366 a          753 c          748 g          372 t
ORIGIN      
        1 cagggcagct cgccgtcgtc attcccggcg atcaggtcct cgaggctggg caggcccgag
       61 gtgcccggtt catcctcgaa cccgcagacc cggccgatgc ctcccggaac gccagagagc
      121 agctcctcga actcctggtc caggaccacg gctttggcgc cttcgcgttc gatcacgtcg
      181 gaaagttgcg ggcccgagaa catggtgttg agcaggatca cgttggcacc gagcttccag
      241 ccggccaggt tggtctcgac gaagccgcgg tggtttcggc acatcacggc cagaccgtcg
      301 ccggggccga ttccgagccc ggcgaaggcg tgagcgaggg cgttgcttcg ccggtgaact
      361 tcggcgaaag tcagggagcc gcgttcatcc tccagataga cccggtcccc gttcttgacg
      421 gcggcgacgc gaatcgcggt cgcgggagtc gtcccgcccc ggatcagggc cgaggcagca
      481 accgggagcg cagccgggcc gagcccgccg accaccccgg ccttgtgcag ggtgcggatt
      541 tcaaatgcct tgttggcgac ggcctgggcg gccgacttga ccgggttcat cgaggaatga
      601 ctctacttga ggatcagtcg agattgacgg cggcaacagc catgcaggcg atcccttcgc
      661 cccggccgat ccaccccatc tgctcattcg tggttgcctt gacgcttacc cggctccccg
      721 ttatttcaga caaccttgcg accatggccg gacggtgggg gcccagcttt ggctgctcgc
      781 agatcaaggt ggcatcgacg tttgtgatcg gcccccgcag catcccgatt accgtgctga
      841 gcaggctgat cgagtcggcg ccttcccagg ccggatcggt atcagggaag acctcgccga
      901 tgtcaccgag cccggcggca ccgaggacag cgtcaatgat cgcgtgggtc agaacgtcgg
      961 cgtccgagtg accgctcagg cccatctcgt gaggaacctc caccccgccg aggatcagcc
     1021 tgcggtcatc cgcgaagcgg tgggagtcgt atccgatgcc gacgctgatt gccatcagcg
     1081 gggatagtcg caggatccgc agtcgacggg gcagctcatg cgcggatgcg cagcggggtt
     1141 cccggccgga cccgcttctt cagggcgagg atcaccttgt tgtagacccg caggcagccg
     1201 tggctggcgc gaaggccgac cgaccaggtt tcactggtgc cgtgcagccc gacgatcccg
     1261 cctcgcggcc agtcggtaat tgccgcatag gcgctggtct cgaaggccca ggggccgaga
     1321 aagtcatcac cggggacgaa acccgcggtc aggtagaagt ggccgagcgg ggtcggtgcg
     1381 tcgggggtgc caaccgcaac cttcgttttc atgaccattc gtttgtggcg gtagagccgc
     1441 aggcgccgct gactgcggtc gatcacgatc cggaatggcc cggcgctccg cagcagacga
     1501 atccgcgaac ccctgaccca gccggtgcga ccgttcgggc ggccggggat cctgaccttg
     1561 agccactcgc cctggccggt ttcccgccgg tcaatcgcga agaagacggt gggccggtag
     1621 tcgaaacgaa acgccttcat caccgccctg accggggcct tcgatatcgg tctcgcccgc
     1681 accgcaatcc ggggggctag cgcctcaccg accgccggta gcgccacctt ctccgcgaag
     1741 gcttcggggc gcgctgcgct ggccgctgcg gagccatccc agacgagcag cagcgaaaga
     1801 gaaaccagca ccccggttac tacaccccgc cggaccatgg ctaaccggtc agctggttac
     1861 cggcgccggc agcgtccagg tcactggccc cgatccggaa ccggcaacgc gtggcattgg
     1921 gcgcggtaac cacgaggtaa ccctgaccgt tgggacggac cctgaggcgg gccatgccct
     1981 tgccgttggt ccgagccgag tcacccgtgc cctgacccct ggcgaccacg gtgatgccga
     2041 tggccggacg gccttcggga gtccgcagac ggacggtcag caccgtggtc ttgttgatgt
     2101 cgatcgtccg cggggtcacg ttcatcgccc ggcaggtttc ctggcgcttc ggtgcgagca
     2161 ccgccttctg gtgactttgc ggcacgctca ccgtccagcg ggcctccgta ctggtcgccc
     2221 gggcggtgaa gtggacctt
//
LOCUS       DAOQFI010000307         2233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_27954_length_2233_cov_3.561983, whole genome shotgun sequence.
ACCESSION   DAOQFI010000307 DAOQFI010000000
VERSION     DAOQFI010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27954_length_2233_cov_3.561983"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLE93_06070"
     CDS             complement(<1..398)
                     /locus_tag="PLE93_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNH86649.1"
                     /translation="MIAPQASATVGGTPLVWLARIADGLDAQVCAKLEYFNPGGSVKD
                     RIGAAMIGAAEARGELVRGESVIVEPTSGNTGIALAMICAARGYQLVLTMPEGMSRER
                     SKLLRAYGAEVQETSSMGGMDEAVTLAEKIA"
     gene            complement(395..793)
                     /locus_tag="PLE93_06075"
     CDS             complement(395..793)
                     /locus_tag="PLE93_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNH86650.1"
                     /translation="MISVTSKSRYAVVALVELAGAGDRPMPVKELAERRGIPDQFLEQ
                     LFSTLRRSGLLTSRRGSKGGYTLSRPASDITVLEVVQALDGKVGQEADEAGGIWAEGV
                     RALRGVFGETTIQDLYEREAEDAAARMYFI"
     gene            complement(873..>2233)
                     /gene="gltX"
                     /locus_tag="PLE93_06080"
     CDS             complement(873..>2233)
                     /gene="gltX"
                     /locus_tag="PLE93_06080"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNH86651.1"
                     /translation="LHIGGARTALYNWLAARHYGGDLVLRIEDTDLERSTPENVAQIL
                     DALRWLELDWDEGPVSQVGRAEDHAAALQKLLDSGAAYRDAATADDVKAWKSEHGADR
                     GYRGTPSEDGSGAIRLRVPDDGDTVVNDLIRGEVRFPNRSYDDFVIARGDGSVLYNFA
                     VAVDDADMGITDVIRGDDLLSNTPKQLLVFEALGIQPPRYAHVPLLHGPDGKKLSKRH
                     GAASVQELRDAGYLPAAIRNYVALLGWGPGDDETLLSTAELIERFRPEDVGRSSAVFD
                     EKKLRWMNGRYMRELPPGEYRESVRSFVEAETVGRPLNEDRFAFGCEIVQEKAQTLQE
                     VWPLIRFLFEPPVQDEKAWRKVMKGDAERVLVAVTEALLAVPAGGEGPNGDGFTIEAV
                     QTALEPLPEKLELGAGKVFQPIRVSITGTTISPGIFESVAALGREETIARIEAARSRL
                     DA"
BASE COUNT          356 a          785 c          694 g          398 t
ORIGIN      
        1 gcgatcttct cggccagggt caccgcttca tccatgccgc ccatcgacga cgtttcctga
       61 acctcggcgc cgtaggcgcg aagcagcttg cttcgttcgc gactcatgcc ttccggcatg
      121 gtcaaaacca gctggtagcc ccgggcggca cagatcatcg ccagggcgat cccggtgttg
      181 ccgctggtcg gctcgacgat caccgactcg ccgcggacca gctctcccct cgcctcggcg
      241 gccccgatca tcgccgcgcc gatccggtcc ttgaccgagc cgcccggatt gaagtactcg
      301 agcttggcgc agacctgggc atccagcccg tccgcgatcc gggccagcca gaccagcgga
      361 gtcccgccga ccgtcgccga tgcctgcggg gcgatcaaat gaagtacatc ctcgccgcgg
      421 cgtcttcggc ttcccgctcg tacaggtcct gaatcgtggt ctcgccgaaa acgcccctca
      481 gggccctgac tccttcggcc cagatgccac cggcctcgtc tgcttcctgc ccgaccttgc
      541 cgtccagcgc ctgaaccacc tccagcacgg tgatgtcact ggccgggcgg gaaagggtgt
      601 agccgccctt gctgccgcgc cggctggtca agaggccgga gcgccgcagg gtcgaaaaca
      661 gctgttcgag gaactgatcc gggattcccc gccgctcggc cagttccttg accggcatcg
      721 gccggtcacc ggctccggcc agctcgacca gagcgaccac ggcgtagcgg gatttggagg
      781 taaccgagat caccgctccc ctttttaccg catcagcccg tcaaaaactt agtaacccgg
      841 tcaggaaaca ggtatttgtc aggaatgggg cactacgcat ccagtcggga gcgggccgct
      901 tcgatccggg cgatggtctc ttcccggccc agagcggcaa ccgattcgaa gattccgggc
      961 gagatggtcg ttcccgtgat cgaaacccgg atcggctgga aaaccttgcc ggctcccagt
     1021 tcgagcttct ccggcagcgg ctcgagtgcc gtctggaccg cctcgatcgt gaagccgtct
     1081 ccgttcgggc cctcgccacc ggccggaacc gccagcagcg cctccgtgac cgcaaccaac
     1141 accctttcgg cgtcgccctt catcaccttg cgccaggcct tttcgtcctg gaccggtggt
     1201 tcgaacaaaa accggatcag gggccagact tcctgcaggg tctgcgcctt ctcctggacg
     1261 atctcgcagc cgaaggcgaa gcgatcctcg ttcagcggac ggccgacggt ctccgcttcc
     1321 acgaaggagc gcaccgattc ccggtattcg ccgggcggca gttcccgcat gtaacggccg
     1381 ttcatccacc ggagcttctt ctcgtcgaag accgccgaag acctgcccac gtcctcgggc
     1441 cggaatcgct cgatcagctc cgcggtcgag agcagggttt cgtcgtcgcc gggcccccag
     1501 cccagcaggg cgacgtagtt gcggatcgcc gccggcaggt aacccgcgtc acgaagctcc
     1561 tggaccgagg cggcaccgtg gcgcttcgag agcttcttgc cgtcgggacc atgcagcagg
     1621 ggcacgtggg cgtagcgggg cggctggatt cccagcgctt cgaagacgag cagttgcttc
     1681 ggggtgttcg agaggagatc gtccccgcgg atcacgtcgg tgatccccat gtcggcgtca
     1741 tcgaccgcga ccgcgaagtt gtagagcacc gaaccgtcgc cccgggcgat cacgaagtcg
     1801 tcgtaactgc ggttgggaaa cctgacttcg ccccggatca ggtcattgac gacggtgtca
     1861 ccgtcatcgg gcacccgcag ccgtatcgcg ccggagccat cttcggaagg ggttccccgg
     1921 tacccccggt ccgccccgtg ttcgctcttc caggccttga cgtcatcggc ggtggcggcg
     1981 tcacggtacg cggcacctga atcgagcagc ttctggagtg ccgcggcgtg gtcctcggcc
     2041 ctcccgacct gggacaccgg accttcgtcc cagtcgagct ccagccagcg cagggcatcg
     2101 agaatctggg ccacgttctc cggggtcgac cgctcgaggt cggtgtcctc gatgcggagc
     2161 acgaggtcgc ctccgtaatg tctggcggcc agccagttgt acagtgcggt tctggctccc
     2221 ccgatgtgta gtg
//
LOCUS       DAOQFI010000308         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28041_length_2230_cov_2.635402, whole genome shotgun sequence.
ACCESSION   DAOQFI010000308 DAOQFI010000000
VERSION     DAOQFI010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28041_length_2230_cov_2.635402"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLE93_06085"
     CDS             complement(<1..582)
                     /locus_tag="PLE93_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86652.1"
                     /translation="MPTTGFDSRRVLGLALISLVISAGAIGCGGGGDDETAAPVPTTT
                     TGTTETASLTQDDLISEGDAICAEANSALGAIESSTADETTKSSQIADIYDGIAQQLG
                     ELGTPSDGEPPTDVIAAAQDLAQGTGDSTTFTTAAGEYGFTDCAEAPESTSYPSTSTG
                     TDSTSTGTDSTGTYTPPATTPEETYTPPTTTAPP"
     gene            705..775
                     /locus_tag="PLE93_06090"
     tRNA            705..775
                     /locus_tag="PLE93_06090"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:737..739,aa:Gln,seq:ctg)
     gene            complement(819..1478)
                     /locus_tag="PLE93_06095"
     CDS             complement(819..1478)
                     /locus_tag="PLE93_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNH86653.1"
                     /translation="MYVIVAGAGKVGVNLTRELLGQGHEVTLIENRRQRYLGVEQLLE
                     HNILYGDASELWVLERAGIERAEMVIAVTGDDEDNILICQVAKEKYGIGRIIARVNNP
                     SNRAHFDLLGIKPVVSATELILRLLEHEVPHHNLVHLLDLEEEEIEIIEIRLGSKSPV
                     VGRSVGEVGTPDNSLVIAIIREGRGVVPNASTVFEEGDDILVVLDPEREEELKQIFTD
                     G"
     gene            complement(1479..1991)
                     /locus_tag="PLE93_06100"
     CDS             complement(1479..1991)
                     /locus_tag="PLE93_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNH86654.1"
                     /translation="MFVLIVGCGRVGSTVARSMLSKGHSVSCLDEDPESHARLEVGLD
                     TSWEEAGGQFTVGTALETQALEGAGIIEADAFVAATNGDNTNIVIAQIAQKRYEVETV
                     IARILDPLRAEWYREQGLRTICPTKVAVEMLEDKLLSAANPTSLDAGSAEFADSPPAP
                     PAGPATGGDE"
     gene            complement(2007..>2230)
                     /locus_tag="PLE93_06105"
     CDS             complement(2007..>2230)
                     /locus_tag="PLE93_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNH86655.1"
                     /translation="IERVRRKGTGIIFAARRLDVDAIVMGAEPPSPIKGGAQLGGIGD
                     YRPEEIGPITAYVLKRAPCRVLLTAPRTA"
BASE COUNT          341 a          711 c          755 g          423 t
ORIGIN      
        1 gggcggtgcg gtggtggtcg gcggggtata ggtctcttcc ggagtggtgg ccggaggggt
       61 gtaggtgccg gttgaatcgg tgccggtcga agtgctgtcg gtaccggttg aagtggacgg
      121 gtacgaggtt gattcggggg cctcggcaca gtcggtgaag ccgtactcgc cggcggcggt
      181 ggtgaaggtg gtcgaatccc cggtgccctg ggcaaggtcc tgggcagccg cgatcacgtc
      241 ggtgggcggt tcaccgtcgc tgggggtgcc gagctccccg agctgctggg cgatgccgtc
      301 gtagatgtcg gcgatctggg aagacttggt cgtctcgtcc gcggtcgatg actcgatcgc
      361 tccgagcgcg gaattggctt ccgcacagat cgcgtcgccc tccgagatca ggtcgtcctg
      421 ggtcagggaa gcggtttcgg tcgtcccggt ggtggtggtc ggcaccgggg ccgccgtctc
      481 gtcgtcgcct ccgccgccgc agccgatggc gccggccgag atgacaagcg agatcagggc
      541 aaggccaaga acgcggcgag agtcaaatcc tgtggtgggc acgtacgcaa ggtaacagaa
      601 ggcgcggtca ggctcaaaag tgcccgattg cggggcaaaa cggccggccc tcccttttca
      661 gactgcactt gcggggccgc gtctatatcc ttgaggacca ccgctgcggg atagaataat
      721 ggtaattcgc gcggttctgg tccgtgaaat cggggttcga gtccctgtcc cgcagccttt
      781 cagcccggcc tcagcgccgg gctttttcat gcccgccctc agccgtcggt gaagatctgc
      841 ttcagctctt cttctcgctc cgggtcgagc acgacgagga tgtcatcgcc ttcttcgaag
      901 accgtcgagg cgttgggcac cacgccgcga ccttcgcgaa tgatcgcgat gaccagtgag
      961 ttgtccggcg tgcccacctc gccgaccgag cggccgacca ccggcgactt gctgccgagg
     1021 cggatctcga tgatctcgat ctcctcttcc tcgaggtcaa gcagatggac caggttgtgg
     1081 tgcggcacct cgtgctcgag caggcgcagg atcagttcgg tcgccgaaac cacaggcttg
     1141 atcccgagca ggtcgaaatg ggcccggttg ctggggttgt tcacacgggc gatgatccgg
     1201 ccgattccgt acttctcctt ggcgacctgg cagatcagga tgttgtcctc gtcatcgccg
     1261 gtgaccgcga tcaccatctc ggcgcgctcg ataccggccc tttcaagcac ccagagctcg
     1321 gaggcatcgc cgtaaaggat gttgtgctcg agcagctgct cgacgcccag gtaacgctgg
     1381 cgacggtttt cgatcagcgt gacttcatgg ccctggccga gcagctcccg ggtcaggttc
     1441 acaccgacct tgccggcccc ggcgacgatc acgtacatct actcgtcccc tccggtcgcg
     1501 ggccccgcgg gcggcgccgg cggcgaatcg gcgaattcgg cgctgccggc gtcgagcgag
     1561 gtcgggttcg ccgcgctcag cagcttgtcc tcgagcatct cgaccgccac cttggtcggg
     1621 cagatcgtcc gcagcccctg ctcgcggtac cactcggccc gcagcggatc gaggatccgg
     1681 gcgatcaccg tttcaacctc ataacgcttc tgtgcgatct gggcgatcac gatgttcgtg
     1741 ttgtcgccat tcgtagcggc cacgaaggcg tcggcctcga tgatccccgc cccttcgagg
     1801 gcctgggttt ccagcgcggt gccaaccgtg aactggccgc ccgcttcctc ccatgaggta
     1861 tcgaggccga cttccagccg ggcgtgcgat tcggggtcct cgtccaggca ggaaaccgaa
     1921 tgccccttgg acagcatcga gcgggcgacg gtggaaccga ccctcccgca tccaacgatc
     1981 aagacgaaca tgggggcatc ctacgctcag gcggttctgg gcgcggtcaa cagaacgcgg
     2041 cagggggccc gtttgaggac gtaggcggtg atcggtccga tttcctcggg ccggtagtcg
     2101 ccgatcccgc ccagttgggc gccgcccttg atcgggctcg gcggctcggc gcccatcacg
     2161 atcgcatcga catcgagccg ccgggccgca aagatgatgc cggttccctt gcgccggacc
     2221 cgctcgatct
//
LOCUS       DAOQFI010000309         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28055_length_2229_cov_3.422723, whole genome shotgun sequence.
ACCESSION   DAOQFI010000309 DAOQFI010000000
VERSION     DAOQFI010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28055_length_2229_cov_3.422723"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLE93_06110"
     CDS             <1..543
                     /locus_tag="PLE93_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86656.1"
                     /translation="GRLGAGVGAAIIVASATAVAALMMLPGKMTVKRGAILIAAPVIG
                     LALLAVLDLTTAGGQGHYSSVLSGGGDSFLDDIRRRTTLAWQQLWNDTMPVITLVCLL
                     AAAYAIRNRDMFKPWAGPIWPAALVGGLAGGLIGSFTEDSGPLLIVVATITLTGVCSY
                     LLGRPEDAGVASGDIPPDKE"
     gene            546..914
                     /locus_tag="PLE93_06115"
     CDS             546..914
                     /locus_tag="PLE93_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH86657.1"
                     /translation="MAVTGTDFVTIPIQDFEASKAFYRDTLGLEEGKQWGNMPAQEFE
                     TGNLTIAIMDPTAFGQDFRPAGGSSVVLQVDDFEASKAELEGKGVKFVTDTIDSGICH
                     QAYFTDPDGNVLGIHHRYAN"
     gene            complement(1007..1093)
                     /locus_tag="PLE93_06120"
     tRNA            complement(1007..1093)
                     /locus_tag="PLE93_06120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1057..1059),aa:Ser,seq:cga)
     gene            1174..1262
                     /locus_tag="PLE93_06125"
     tRNA            1174..1262
                     /locus_tag="PLE93_06125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1208..1210,aa:Ser,seq:gga)
     gene            complement(1271..1798)
                     /gene="tadA"
                     /locus_tag="PLE93_06130"
     CDS             complement(1271..1798)
                     /gene="tadA"
                     /locus_tag="PLE93_06130"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNH86658.1"
                     /translation="MNLEQESGDLAPGSRNFGPESSDPVLRAGDEEMMRLALAAARKA
                     AEHDDVPIGAVIARDGIPLAMAGNRREADRDPTAHAELIAIRAAASELGGWRLPETTL
                     FVTLEPCAMCAGAIVLARIPRVVFGATDPKAGAAGSIMNVLDDPALNHRPEVESGLMA
                     EECGDLLREFFSARR"
     gene            1911..>2229
                     /locus_tag="PLE93_06135"
     CDS             1911..>2229
                     /locus_tag="PLE93_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86659.1"
                     /translation="MNRSAHLSLALFGALLAATCAVVLMLPTAAPADETAPPGTPVSD
                     APADQVAGQATTVLDDGSTAVAGEVLVGHYGEAAPTTVEIPESADPAMVAEAQADHSS
                     EMPV"
BASE COUNT          361 a          727 c          742 g          399 t
ORIGIN      
        1 ggccgcctcg gcgccggtgt cggagccgcg atcatcgtcg cctcggcaac cgcggtcgcg
       61 gctctgatga tgctgcccgg aaagatgacc gtgaaaaggg gggcgatact gattgccgcg
      121 ccggtcatcg gtctggcgct gctggccgtg ctcgacctga ctactgccgg gggtcaaggc
      181 cactacagct cggttctttc cgggggcggt gacagtttcc tcgacgacat ccgccgtcgc
      241 accaccctcg cctggcagca gctctggaac gacaccatgc cggtgatcac cctggtctgc
      301 ctgctcgccg ccgcctacgc gatccgcaat cgcgacatgt tcaaaccatg ggccggcccg
      361 atctggccgg cggccctggt cggcggtctg gccggcggcc tgatcggttc cttcaccgag
      421 gactcgggac cactcctgat cgtggtcgcg acgatcaccc tgaccggggt ctgctcgtat
      481 ctgcttggac ggccggagga tgccggggta gcatcggggg acatcccccc agacaaggag
      541 tgaaagtggc tgttacaggt accgacttcg tgaccattcc gatccaggac ttcgaggctt
      601 cgaaggcttt ctaccgcgac accctcggcc tcgaggaggg caagcagtgg ggcaacatgc
      661 cggcccagga attcgagacc ggcaacctga cgatcgcgat catggacccg accgccttcg
      721 gccaggattt ccggccggcc ggcggcagca gcgtcgtgct gcaggtcgat gacttcgaag
      781 cctccaaggc tgaactggaa ggcaagggcg tcaagttcgt caccgacacg atcgacagcg
      841 ggatctgcca ccaggcctac ttcacggacc cggacggaaa cgtcctcgga atccaccaca
      901 gatacgcgaa ctgagaccgc tcccggaatc agtgagatcc gggtttctcg tcacattggc
      961 gaaaaaatcg gatctcgatg aagagaggac cttacggcgg cttatacgga gggggtggga
     1021 ttcgaaccca cggtacgccg aaacgcacaa cagttttcga gactgcccga ttcaaccact
     1081 ctcgcacccc tccgggggtg acttggaagg ctagcgattc gcgcgggacg gggcgccttc
     1141 ggcggctgtt ctcgggtacc cttccctgtc cccggagagg tgtccgagcg gcttaaggag
     1201 cacgattgga aatcgtgtgg gcgggatctc tcgcctcgtg ggttcgaatc ccaccctctc
     1261 cgttccgggg ttagcgacgg gccgagaaga actcgcggag gaggtctccg cactcttcgg
     1321 ccatcaggcc tgactcgact tcgggccggt ggttgagggc cgggtcgtcg agtacgttca
     1381 tgatcgagcc ggccgccccc gccttggggt cggtggcacc gaagaccacc cgggggatgc
     1441 gggcgaggac gatcgctccg gcacacatcg cgcatggctc gagggtcacg aagagcgtgg
     1501 tttccgggag gcgccagcca cccagttcgg aggcggccgc gcgaatcgcg atcagctccg
     1561 cgtgcgcggt cgggtcccgg tcggcttcgc gccggttccc ggccatagcc agggggatgc
     1621 cgtcccgggc gatcacggcg ccgatcggga catcgtcatg ttccgctgcc ttgcgggcgg
     1681 ccgcgagggc cagccgcatc atctcctcat cgccggcccg aagcacggga tcactgcttt
     1741 cagggccgaa attgcggctt ccgggtgcca ggtcgccgct ttcctgttcc aggttcaccg
     1801 gttcagggta agtcatgggg cagggcgagg ttccgccctt ttcgggagtt cgagcaaccc
     1861 ggcggggttt ggggtgttct ttcagtaccg gtcgcctact gccttcttcc ttgaatcgat
     1921 cagcccacct cagcctcgcg cttttcggcg ccctgctggc cgccacctgc gcggtcgtcc
     1981 tgatgctgcc gacggcggcc ccggctgacg aaaccgcgcc tcccgggacg cccgtttccg
     2041 acgctccggc ggatcaggtt gccggccagg cgacgaccgt gctcgatgat ggttcgactg
     2101 cggttgccgg cgaggtcctg gtcggccatt acggcgaggc agcaccgacg acggttgaaa
     2161 tcccggaatc cgcggacccg gcgatggtcg ccgaggctca ggctgatcac tccagcgaga
     2221 tgccggtgg
//
LOCUS       DAOQFI010000310         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28193_length_2223_cov_3.166974, whole genome shotgun sequence.
ACCESSION   DAOQFI010000310 DAOQFI010000000
VERSION     DAOQFI010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28193_length_2223_cov_3.166974"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLE93_06140"
     CDS             <1..472
                     /locus_tag="PLE93_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNH86660.1"
                     /translation="GVVIRRLTTDPALQDRWRDHRPEAADMNREIDELLRIDDPFTVS
                     RRVTTCPVSFGDQAIPAGARIRLDWAEANRDPEVVGDPEAFRPVENAGHNLVYGIGPH
                     VCPGRGLATLEIRVVVEELLARISLEPDPELEPVRAEAPQGGFESVPVRLRELA"
     gene            492..>2223
                     /gene="ileS"
                     /locus_tag="PLE93_06145"
     CDS             492..>2223
                     /gene="ileS"
                     /locus_tag="PLE93_06145"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNH86661.1"
                     /translation="MSSFRPVDPKQSFPEMEERINAAWKESRVFERQLEQREDAEVWS
                     FYEGPPTANGRPGSHHVLSRVFKDIYPRYRAMRGYRVPRKAGWDCHGLPVELEVEKQL
                     GISSKQEIEDYGIEKFNQLCRESVFTYVEEWNRLTDRIGFWIDLDDPYVTMDSEYIES
                     VWWSLKELYESDRLYEGHRVSPYCPRCGTALSSHEVAQGYRDVIDPSVYVRFPLTGRD
                     ASLLIWTTTPWTLPGNYAVAVDPEVTYAEVDFEGETLILAGNLIEKVLGEGVVAERTF
                     KGEELLGQGYEAPFPGMAENSGVFKVISGDFVTTDDGTGMVHIAPAFGEDDYRAAIAN
                     GLYDPSGKGAGGTLFNPVGLDGRFDARVTGFEGEFVKDPEVTERLIGHLRENGRLLKA
                     SDYEHAYPHCWRCGTPLLYYAKASWYIKTAEAKGAMLAGNEKIGWHPEHIKDGRFGRW
                     LENNVDWALSRDRYWGTPLPIWECSDPACEESFCAGSIEELREKAGQVPEDLHRPFID
                     EVTWNCDCGGEMKRVVSVIDTWYDSGAMPFAQFHYPFEGKEEFEQRFPADYICEAIDQ
                     TRGWFYTLLAE"
BASE COUNT          402 a          733 c          733 g          355 t
ORIGIN      
        1 cggggtggtg atccgccgcc tcacgacgga ccccgccctg caggaccgct ggcgcgatca
       61 ccgtcccgaa gcggccgata tgaaccgcga gatcgacgag ttgctccgca tcgacgatcc
      121 cttcaccgtc agccggcggg tcaccacctg cccggtctcg ttcggtgacc aggcaatccc
      181 ggccggggcc cggatacgac tcgactgggc cgaagccaac cgtgaccccg aggtggtcgg
      241 tgatcccgaa gccttccgtc cggtcgagaa cgccggccac aacctggtct acgggatcgg
      301 gccccacgtc tgccccggcc gcgggctggc cacgctcgag atccgggtcg ttgtcgagga
      361 gctgctcgcc aggatcagcc tcgaaccgga tccggaactg gaaccggtcc gggccgaggc
      421 gccgcagggc ggtttcgagt cggtgccggt tcgactccgg gagcttgcgt gatccataaa
      481 ctcccccgcc catgtcgtct tttcgcccgg ttgatccgaa gcagtccttt cccgagatgg
      541 aggagaggat caacgctgcc tggaaggaaa gccgggtctt cgagcggcag ctcgagcaac
      601 gggaagacgc cgaggtctgg agtttctacg aggggccccc gaccgcgaac gggcggcccg
      661 gctcccacca tgttctgagc cgggtcttca aggacatcta cccgcgctac cgggcgatgc
      721 ggggctaccg ggtgccgcgc aaggcgggct gggactgtca cggactgccg gtcgaactcg
      781 aggtcgagaa acagctcggc atctcctcca agcaggagat cgaggactac gggatcgaga
      841 agttcaacca gctctgccgc gagtcggtct tcacctacgt cgaggaatgg aaccggctga
      901 ccgaccggat cggtttctgg atcgacctcg acgacccgta cgtgaccatg gactccgaat
      961 acatcgagtc ggtctggtgg tcgctgaagg agctctacga atcggaccgg ctctacgaag
     1021 gtcaccgggt ctcgccctac tgcccgcgct gcggcaccgc tctctcttcg catgaggtgg
     1081 cgcagggtta cagggacgtg atcgatccct cggtctacgt gaggttcccg ctgaccggcc
     1141 gggacgcatc gctcctgatc tggaccacca ccccctggac cctgcccggc aactacgcgg
     1201 tcgcggtcga cccggaggtg acctacgccg aggtcgattt cgagggcgag accctgatcc
     1261 tggccgggaa cctgatcgag aaggtgctgg gcgagggcgt ggttgccgaa cggaccttca
     1321 agggagagga gctgctcggt cagggctacg aagcaccctt cccgggcatg gccgagaact
     1381 cgggagtgtt caaggtgatc agcggcgact tcgtgaccac cgacgacggc accggcatgg
     1441 tgcatatagc ccctgccttc ggcgaggacg attaccgggc ggcgatcgcc aacggcctct
     1501 acgacccttc gggcaagggc gccggaggaa ccctgttcaa cccggtcggc ctcgacggcc
     1561 ggttcgacgc ccgggtgacc ggattcgagg gcgagttcgt caaggacccg gaagtcacgg
     1621 aaagacttat cggtcacctg cgagagaacg gccgcctgct gaaggccagt gactacgagc
     1681 acgcctaccc gcattgctgg cgctgcggca ccccgctgct ctactacgcc aaggccagct
     1741 ggtacatcaa gaccgccgag gccaagggcg cgatgctggc cggaaacgaa aagatcggct
     1801 ggcacccgga gcacatcaag gacggccgct tcggccgctg gctcgagaac aacgtcgact
     1861 gggccctttc ccgtgaccgc tactggggca ccccgctgcc gatctgggaa tgctctgacc
     1921 cggcctgcga agagagcttc tgcgccggct cgatcgagga gctccgcgaa aaggccggtc
     1981 aggtgcccga ggatctccac cgccccttca tcgacgaggt gacctggaac tgcgactgcg
     2041 gcggcgagat gaaacgggtg gtcagcgtga tcgacacctg gtacgactcg ggcgcgatgc
     2101 ccttcgccca gttccactac cccttcgagg gcaaggaaga gttcgagcag cggttcccgg
     2161 ccgactacat ctgcgaggcg atcgaccaga cccgcggctg gttctacacg ctgctggccg
     2221 agt
//
LOCUS       DAOQFI010000311         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28372_length_2216_cov_2.121703, whole genome shotgun sequence.
ACCESSION   DAOQFI010000311 DAOQFI010000000
VERSION     DAOQFI010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28372_length_2216_cov_2.121703"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLE93_06150"
     CDS             complement(<1..747)
                     /locus_tag="PLE93_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86662.1"
                     /translation="MRIHLRLVLLFLVAAGLIGGVLALTVLNTNEGSGSPDQLTPTGP
                     TGPTGPTSTTGPTAADTTAGFGQTLPIEGPLLGVNLTAYTSDGYSKPSVRKAIKTLAD
                     LGGTAITLVPTWYMKSSSANAIEPDEEKSPSDESLAKVISWIRQAGMQVIMKPHVDVI
                     DDSYRGDIQPADREAWFRSYGTFIDHFASFSTSHSVDLFVAGTELKTMSSETDRWRAV
                     IQTVRDRFTGPITYAANWDEVDQVQFWDDLD"
     gene            complement(833..1228)
                     /locus_tag="PLE93_06155"
     CDS             complement(833..1228)
                     /locus_tag="PLE93_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNH86663.1"
                     /translation="MLNILASYDLGDALLTTLAIFFFVIWIWIVITILMDIFRDHELS
                     GWWKAVWVIFLIFIPFLTALVYLIARGSGMRERAIKEQADAKKHFDEYVRETAQTTPV
                     DELHKLDQLRQSGGLTDEEYAKMKAKLID"
     gene            complement(1323..1922)
                     /locus_tag="PLE93_06160"
     CDS             complement(1323..1922)
                     /locus_tag="PLE93_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNH86664.1"
                     /translation="MSTLSNTVDSPRVPVLADLFANTWIKNAVLVLGGALFMALFQQI
                     SFSMEPFPAPITGGTLGVLLIGSALGSRRGAAALLLYLMMGLILPVYSEGREGAEVLF
                     NTASTGYFLGYLIAAYVVGYLAEHGRDRKFQTAFVSFLFCQLIIFACGVLGLMIVMNF
                     DFPTAFHDGFVLYIPIELIKAAIGAILLPAAWKLAEKTR"
     gene            1983..>2216
                     /locus_tag="PLE93_06165"
     CDS             1983..>2216
                     /locus_tag="PLE93_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86665.1"
                     /translation="MPGTVTENAVSELRRVDAIRYVTALREGGSLPGLVEGDDDGTWV
                     VKFHGAGQGPGALVSEVVAGEIARQIGLPVPELV"
BASE COUNT          450 a          658 c          732 g          376 t
ORIGIN      
        1 atcgaggtcg tcccagaatt gaacctgatc gacttcgtcc cagttggcgg cgtaggtgat
       61 cgggcccgtg aagcggtcac ggaccgtctg gatcaccgcc cgccagcgat cggtctccga
      121 ggacatcgtc ttgagctcgg tcccggccac gaagagatcg accgaatggg aggtcgaaaa
      181 cgacgcgaaa tggtcgatga aggtgccgta ggaacggaac caggcttcgc ggtcggcggg
      241 ctggatgtca ccgcggtagg aatcatcgat cacatcgaca tgcggcttca tgatgacctg
      301 catcccggcc tgcctgatcc agctgatcac cttcgcgagg ctctcgtcgg atggggactt
      361 ttcctcgtcc ggctcgatcg cgttggcgct cgaactcttc atgtaccagg tcggcaccag
      421 ggttattgcg gttccgccca ggtcagcgag ggtctttatc gctttgcgga ccgacggctt
      481 cgagtagccg tcgctggtgt aggcggtgag attcacaccc agcagcggtc cttcgatcgg
      541 cagcgtctgg ccgaaaccgg cggtcgtgtc tgctgccgtc ggcccggtgg tcgaggtcgg
      601 cccggtgggc ccggtcggcc cggtcggagt cagttggtcg ggcgagccgc ttccctcgtt
      661 cgtgttcagc acggtcaaag cgagcacccc tccgatcagc cctgcggcca cgagaaagag
      721 cagaaccagt cggagatgaa tacgcacgcc ctcaccctac gcaatgccga acctgacgca
      781 aaaaaagagg ggccggcttt acccggcccc tccaaagcgc aagaaaggcc gctcagtcga
      841 tcagcttggc cttcatcttc gcgtactcct cgtccgtcag gccaccgctc tgacgcagct
      901 ggtcgagctt gtggagctcg tcgaccggcg tggtctgggc ggtttcccga acgtactcgt
      961 cgaaatgttt cttggcgtca gcctgttcct tgatcgccct ttcccgcatc ccggaaccac
     1021 gggcgatcag gtagaccagc gcggtcagga acgggatgaa gatcaggaag atcacccaca
     1081 cggccttcca ccagccggag agctcatggt cacggaagat gtccatcagg atcgtgatga
     1141 cgatccagat ccagatcacg aagaagaaga tcgcgagcgt ggtgagaagc gcgtcaccaa
     1201 ggtcgtagga ggccaggata tttagcaagt ctgttccttt caggaaagaa gacggggacg
     1261 aacacgctca ggctacaccc gatcggcccg tccctcatca ccccggacgg gtgatcaggg
     1321 attcagcggg ttttctcggc cagcttccag gccgccggca gcaggatcgc gccgatcgcg
     1381 gccttgatca gctcgatcgg gatgtagagc acgaagccgt cgtggaaggc ggtcgggaaa
     1441 tcgaagttca tcacgatcat cagcccgagg acaccgcagg cgaagatgat caactggcaa
     1501 aagaggaagg agacgaacgc ggtctggaac ttgcggtccc gcccgtgctc ggccaggtag
     1561 ccgaccacgt aggcggcgat caggtagccg aggaagtaac cggtcgaagc cgtgttgaag
     1621 aggacttcag cgccttcccg gccttcggaa tagaccggca ggatcaggcc catcatcagg
     1681 tagaggagca gggccgcagc gccacgccgg gagccgaggg ccgagccgat cagaaggacg
     1741 ccgagggtgc caccggtgat cggggccggg aacggctcca tcgagaacga gatctgctgg
     1801 aacagcgcca tgaagagcgc accaccgagt accaggacgg cattcttgat ccaggtattg
     1861 gcgaacaaat cggcgaggac gggaacacgg ggagagtcaa ccgtgttgga aagcgtggac
     1921 atcgagaccc tttcttaggc gtgaagaacg gctgaagcct acccggatgc gaagctatag
     1981 cgatgcccgg cacggtcacc gagaacgcgg tcagtgaact gaggcgggtc gacgcgatcc
     2041 gctacgtaac cgcgctgcgc gagggcgggt cgctgccagg cctggtcgag ggtgatgacg
     2101 acggcacctg ggtggtcaag ttccacggtg ccgggcaggg cccgggcgcc ctggtctccg
     2161 aggtcgtggc cggagagatc gcccgccaga tcggccttcc ggtgcccgag ctggtg
//
LOCUS       DAOQFI010000312         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28545_length_2208_cov_3.215049, whole genome shotgun sequence.
ACCESSION   DAOQFI010000312 DAOQFI010000000
VERSION     DAOQFI010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28545_length_2208_cov_3.215049"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLE93_06170"
     CDS             complement(<1..615)
                     /locus_tag="PLE93_06170"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNH86666.1"
                     /translation="MSENLRIGLLGRGTVGSAFDELVAERADQVEAATGRRPEVTGVL
                     TRSQGDFGEILPDADVIVELMGGTDTAREHVLAALRAGVPVVTANKQLLAQHGAELFA
                     AAEEGGVQLRFEAAVAAVVPVIRVVQESFGAVEFSKVFGIVNGTTNFILSEMAATGAS
                     YAEVLKRAQELGYAEADPTEDVNGADAAAKMAILARLAFHTPVTL"
     gene            complement(612..1865)
                     /gene="lysA"
                     /locus_tag="PLE93_06175"
     CDS             complement(612..1865)
                     /gene="lysA"
                     /locus_tag="PLE93_06175"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNH86667.1"
                     /translation="MPYPVGTTTNDAGHLEIGGCDVIELAREFGTPSYLFATAEMRER
                     ARAYQRAFAAHTDHFEVLFASKALPVTAAYRLFAEEGLSVDVASGGELTMALTAGFDP
                     ARIYMHGNNKTDAELVLAGNSGVGHLIVDSFDEIDRCERVLKRKQDVLIRVTPGIKPS
                     THSFIQTGQLDSKFGFGLEDGTAAKAITRVLASDTLNLVGLHFHIGSQIFEIEPFRLA
                     VEALGELRGDWCSILDLGGGLGISYDEADSPPPIEEYVNLKVQAVRDVFGDEVKILIE
                     PGRSLVGTAGVTIYRIGTVKEIPGVRTYVAVDGGMSDNLRPMLYDAKYTAVIADRPDD
                     PAGTTVTVAGSHCESGDILIRDVELANPRVGDILLTPATGAYGHSMSNNYNGMPRPPV
                     IFCEDGEATVVVRRETWDDLLRRDM"
     gene            1921..>2208
                     /locus_tag="PLE93_06180"
     CDS             1921..>2208
                     /locus_tag="PLE93_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86668.1"
                     /translation="MASSARRPINAPPGSPQGTGVPWLGLSILLVLLLILAAFVAYFF
                     FDVGKDEGGDSSSGVTELETGATGGQGAIVGHSNGPLGFPSAATRNTTRISG"
BASE COUNT          367 a          724 c          731 g          386 t
ORIGIN      
        1 aagggtgacc ggggtgtgaa aggcgagccg ggcgaggatc gccatcttcg cggcggcgtc
       61 ggccccgttc acatcctcgg tcgggtcggc ctcggcgtaa ccgagttcct gggcccgctt
      121 cagcacctcg gcgtaggagg caccggtggc ggccatctcg gaaaggatga agttcgtcgt
      181 cccgttgacg atgccgaaga ccttgctgaa ctcgaccgcg ccgaagcttt cctgaaccac
      241 gcggatcacc ggcacgaccg cagccactgc ggcctcgaag cggagctgga ccccgccttc
      301 ttcggcggcg gcgaagagct cggccccgtg ctgggcgagc agttgcttgt tcgcggtgac
      361 caccggcacg ccggccctga gcgccgccag cacatgttcg cgggcggtgt cggtgccgcc
      421 catcaactcg acgatcacgt cggcatcggg caggatctcg ccgaaatcac cctggctgcg
      481 ggtcaggacc ccggtcacct ccggccgacg gccggtcgcc gcctcgacct ggtcggcccg
      541 ttcggcaaca agttcatcga aggcggagcc gacggttccc cgtccgagca ggccgatgcg
      601 caggttctcg ctcacatgtc cctcctgaga aggtcatccc aggtttcgcg tcgcaccacc
      661 acggtggctt cgccgtcttc acagaaaatc acgggcggcc gcggcattcc gttgtagttg
      721 ttcgacatcg agtgcccgta ggcgccggtc gccggggtaa gcaggatgtc ccccacccgg
      781 gggttcgcga gctcgacatc acggatcagg atatctccgg actcgcaatg tgaaccggcg
      841 acggtgacgg tggtcccggc ggggtcatcg ggccggtcgg cgatcaccgc cgtgtacttc
      901 gcgtcataga gcatcggccg gaggttgtcg gacatgccgc cgtccaccgc gacataggtc
      961 cggaccccgg ggatctcctt cacggtcccg atccggtaga tcgtcacccc ggcggtacca
     1021 accagcgagc ggcccggctc gatcaggatc ttgacctcgt ctccgaagac gtcgcgaacc
     1081 gcctggacct tgaggttcac gtactcctcg atcggcgggg gtgaatcggc ctcgtcgtaa
     1141 gagatcccga ggccgccgcc gaggtcgagg atcgagcacc agtcgccgcg caactcgccg
     1201 agtgcctcga ccgccaggcg gaagggctcg atctcgaaga tctgggagcc gatgtgaaaa
     1261 tgaaggccga ccagattcag ggtgtccgag gcaagcaccc gggtgatcgc tttggcggct
     1321 gtaccgtcct ccagcccgaa gccgaacttc gagtcaagct ggccggtctg gatgaaggag
     1381 tgggttgacg gtttgatgcc cggggtgacc cggatcagca cgtcctgctt gcgcttcagc
     1441 acccgctcgc atcgatcgat ttcgtcaaac gagtcgacga tcaggtggcc gacccccgag
     1501 ttgccggcga gcaccagctc ggcgtcggtc ttgttgttgc cgtgcatgta gatcctggcc
     1561 gggtcgaaac cggcggtaag ggccatggtc aactcgccac ccgaggcgac atcgaccgaa
     1621 agtccttctt cggcgaagag ccggtaggcg gccgtgaccg gcagggcctt gctcgcgaaa
     1681 agcacttcga agtgatcggt atgtgcggcg aaggcacgct gataggcccg ggcccgttcc
     1741 cgcatttcgg ccgtggcgaa gaggtaggac ggggtgccga actcgcgggc gagttcgatt
     1801 acgtcgcagc ctccaatctc gaggtggccg gcgtcattgg tggttgtccc aaccgggtat
     1861 ggcatcgccg ccattcttac gatttccggt gaacgacacc gtttggatac ggttcacgcc
     1921 gtggcaagct ccgcaagacg gccgatcaac gcaccgccag ggagtccgca gggcaccggg
     1981 gtgccctggc tcggcctctc gatcctgctc gtcctgctgc tgattctggc cgccttcgtc
     2041 gcctatttct tctttgacgt gggcaaggac gaagggggcg attcctcctc aggcgtgacc
     2101 gagctggaaa ccggcgcaac cgggggccag ggcgcgatcg tcggtcactc gaacggcccg
     2161 ctcggtttcc cctcggcggc cacccgcaac acgaccagga tcagcggc
//
LOCUS       DAOQFI010000313         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28560_length_2207_cov_3.860130, whole genome shotgun sequence.
ACCESSION   DAOQFI010000313 DAOQFI010000000
VERSION     DAOQFI010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28560_length_2207_cov_3.860130"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PLE93_06185"
     CDS             complement(<1..1144)
                     /locus_tag="PLE93_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86669.1"
                     /translation="MRNPGQSRSLPWVLLGLLMLGSGAILLSVTSGINFVTDEWNLLF
                     LRSGWTPDSFLEPFHEHVIIAPTLIYKVLQGTLGMDSNRPFQLAALIMFLAIGPLLFI
                     WMRRRIGDWAALIGVALILFLGAAFEDLLWAFQIGYFGSLAFGLAALIALDRDDRRGD
                     VAAAILLVLSLSFSSLGMPFVAGAAAEWLTNPRERGKRWLVPAAAAAFYGIWWLGWGH
                     SAETTFSLANIPEAPRFIFDAASAGATSLFGLATGDGSEPDQPHLIWGRIILVALLAL
                     SGLRLWRLKRIPPGVIIAATVALSFFALSALAQSDSRLPTSSRYQLPGALFLLLFFTE
                     VLRGLKLKPPALVAGAAVTIAAAVMGVGLMSDQSRDRWVPVSTSTST"
     gene            complement(1170..>2207)
                     /locus_tag="PLE93_06190"
     CDS             complement(1170..>2207)
                     /locus_tag="PLE93_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86670.1"
                     /translation="IGIWILWWIGWGHQADSQAGLSGLSGLPGYLWKAAPAGFTSLAG
                     LATDDGTMPDQPHLIWGRLLCLAALAAAGWRIYRLGRVPRELWVAIAAAATFFVLSGL
                     NASDFRLPTSSRYQLPSALFIVLIAANLLRGVKIPGRALVASAILAALAVFGGARLME
                     EQATERWQPTSRSARAALGAIDLAGPAVIPGYQIDLRTNPEQDRLIPIDLYRETTASH
                     GSPGMDASEIEGAAPQYRLQADLALIGATGIHLSGLPVRPSGPCQRVRGSNNLFGPVP
                     LQSGGYLIMNRGKDQLAVALSRFSDLPGVSAGAILPETMAGLDLPQDNSTRPWQAVFF
                     GEGPVRICPTG"
BASE COUNT          414 a          727 c          789 g          277 t
ORIGIN      
        1 aggtgctggt cgaggtcgag acaggaaccc acctgtcccg ggactggtcg ctcatcagcc
       61 cgacccccat gaccgcggcc gcgatcgtga ccgccgcgcc ggcgaccagg gccggcggct
      121 tcagcttcag cccgcgcagg acctcggtga agaagagcag cagaaagagg gcccccggca
      181 gctggtagcg gctggaggta ggcaggcggc tgtcactctg ggcgagcgcg gaaagggcga
      241 agaaggagag cgcgacggtt gcggcgatga ttaccccggg agggatccgc ttcagccgcc
      301 agagccgcag cccggagagg gcgagcagag ccaccaggat gatccggccc cagatcagat
      361 gaggctgatc gggttcactg ccgtctccgg tggcgaggcc gaagagggaa gtggccccgg
      421 ccgaggcggc gtcgaagatg aagcgcgggg cttcagggat gttggcgagg ctgaaggtgg
      481 tctcggcgct gtggccccag cccagccacc agatgccgta gaaggcggcg gctgctgcgg
      541 gaaccagcca gcgcttcccc cgctcacggg gattggtcag ccactcggcg gccgcgccgg
      601 ccacgaaggg catgccgagc gaagagaagc tgagggaaag gaccagcagg atcgccgccg
      661 cgacatcccc ccggcggtcg tcgcgatcga gtgcgatcag cgcggccagg ccgaaggcca
      721 gcgacccgaa gtagccgatc tggaaggccc agagcaggtc ctcgaaggcc gcccccagaa
      781 agaggatcag ggcgaccccg atcagcgccg cccagtcccc gatccgtctc cgcatccaga
      841 tgaagagcaa cgggccgatc gccaggaaca tgatcagcgc agcaagctgg aagggccggt
      901 tcgaatccat ccccagggtc ccttggagaa ccttgtagat cagggtgggg gcgatgatca
      961 cgtgctcgtg gaagggctcc aggaagctgt ccggggtcca gccggaccgg aggaagagca
     1021 ggttccattc gtcggtgacg aaattgatcc ccgaggtgac cgagagcagg atcgctcccg
     1081 aaccgagcat cagcagaccg agcagaaccc acggcaggga acgggactgg cctgggttcc
     1141 gcatgccggt caacctactc ccggaaggct cacccggtcg ggcagatccg gaccgggccc
     1201 tcgccgaaga agaccgcctg ccagggcctc gtcgaattgt cctgcggcag gtccagcccg
     1261 gccatcgtct ccgggaggat cgcgccggca ctcaccccgg gcaggtcgga aaaccggctc
     1321 aaggcaaccg ccagctggtc cttgccccgg ttcatgatca ggtagccgcc cgactggagg
     1381 gggaccggcc cgaagaggtt gttcgacccg cgcaccctct gacaggggcc ggagggacgg
     1441 accggcaggc cactgagatg gattccggtc gccccgatca gggcgaggtc ggcctgaagc
     1501 cggtactgag gggcggcacc ttcgatctcg ctggcgtcca tccccggtga tccgtggctg
     1561 gcggtcgtct cccggtagag atcgatcggg atcaggcggt cctgttcggg attcgtccgc
     1621 aggtcgatct ggtatcccgg gatcacggcc ggaccggcca ggtcgatcgc acccagcgcg
     1681 gcccgggccg agcgcgaggt cggttgccag cgttccgtcg cctgttcctc catcaacctg
     1741 gctcccccga agaccgccag cgcagccagg atcgccgagg cgacaagggc gcgtccgggg
     1801 atcttcaccc cgcgcagcag gttggccgcg atcaggacga tgaaaagcgc cgagggaagc
     1861 tggtaccggc tggaggtcgg caagcggaaa tcactggcgt tgaggccgga gagcacgaag
     1921 aaggtagcgg cggccgcgat cgccacccag agctcccgcg gcacccgccc cagccggtag
     1981 atccgccagc ccgccgcggc cagggcggcc aggcaaagca ggcgccccca gatcaggtga
     2041 ggctggtcgg gcatggtgcc gtcatccgtc gccagcccgg ccagtgaggt gaaaccggcc
     2101 ggggccgcct tccagaggta gccgggcaga ccactcagtc cggagagccc ggcctgcgag
     2161 tcggcctgat ggccccaccc gatccaccac aggatccaga tgccgat
//
LOCUS       DAOQFI010000314         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28637_length_2204_cov_2.706375, whole genome shotgun sequence.
ACCESSION   DAOQFI010000314 DAOQFI010000000
VERSION     DAOQFI010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28637_length_2204_cov_2.706375"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..>2204
                     /locus_tag="PLE93_06195"
     CDS             135..>2204
                     /locus_tag="PLE93_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH86671.1"
                     /translation="MPNSIAVLGDSMSVATGINVIPSVTQPESSWSTGTNSSVNSLYQ
                     RILALNPAISGKNRNMAANGEDMTDAPAQANATPADTELVTMQLGGNNLCKDSVAQMT
                     SVAEYRARFVETLDILQSRMPNALIQIESVPDIYNLWFLRGAPNPPNDQPSSRAGTAR
                     VFWDTLAVIPCKSLVLDPTDMSQAAIDRRNAVRQRDLDYNKALAEECEARLRCRFDRY
                     ATFAFSSNRVVPGDLTGPYLPRAQWQFVDDDISTVDHFHPGLSGQRKMAQAAWESGYN
                     FTDSTMPTGAASISPAPLQSGTSFLPSTVSTTWSDAAGIKGIQYRTHFNGTASAWQTQ
                     LGTTTIPVNQTETYGYLTQKTFSVTVTTPGTTYVESRAMDVNGNLSASKVTEVNYDPN
                     AIAAPQITSTPPSITASNAASFSFTGAISGTSYECSLDGGAFSDCSPPKSYSSIADGQ
                     HEFRVRLKSAVASGPAVSYQWQIDSSAPPAPTIHTGPAANTKLTVADFTFSGTEAALE
                     CSLDGATFASCTSPRNVFVPTDGVHRFEVRQKTITGVPGTSAVYTWTVDNTKPPAPVF
                     GEGPSSPTNSTSASIQFTGEPGGTYSCQLSGPGGLQINNCTSPRNLAGLAQGEYTLSV
                     SQKDQLGNQGPAASYSWTVDLTAPVAPQVGSGPPARTASRDATVTFTGEAGGHFEGVQ
                     GGVLRRQG"
BASE COUNT          398 a          823 c          639 g          344 t
ORIGIN      
        1 agggagactt gaccagcggg gtcccagtca tcagtcgcgt attgattctt gccgcgacgc
       61 ttgccgtgtt tgcaaacttt gccgccggct acgcctctgc cgcgcctcct accccgaccg
      121 ccgccgatcc gggcatgccg aactcaatcg cggtgctggg ggattcgatg tcggtggcga
      181 ccggcatcaa cgtgatccct tcggtcaccc agccggagag ttcctggtca accgggacca
      241 actcgtcggt caacagcctc taccagcgca tcctcgccct gaacccggcg atctccggaa
      301 agaaccggaa catggccgcc aacggcgagg acatgaccga cgctccggcc caggcaaacg
      361 cgactccggc cgacaccgag ctggtgacca tgcagctcgg cggcaacaat ctctgcaagg
      421 actcggtcgc ccagatgacc tcggtcgccg agtaccgggc ccggttcgtc gagaccctgg
      481 acatccttca gagccgcatg ccgaacgcgc tgatccagat cgagagcgtg cctgacatct
      541 acaacctctg gttcctgcgc ggggcgccga acccgcccaa tgaccaaccc tccagccggg
      601 ccggcacggc gagggtcttc tgggacacgc ttgcggtgat cccctgcaag tcgctggttc
      661 tcgacccgac cgatatgagc caggcggcga tcgaccggcg caacgcggtc cgccagcggg
      721 acctcgacta caacaaggcc ctggcggagg aatgcgaagc ccgcctgcgc tgccgcttcg
      781 accgttacgc gaccttcgcc ttctcatcca accgggtcgt ccccggggac ctcaccggtc
      841 cctacctgcc tcgcgcccag tggcagttcg tcgatgatga catcagcacc gtcgaccatt
      901 tccatcccgg cctttccggc cagcgcaaga tggcccaggc agcctgggag tccggctaca
      961 acttcaccga ctcgaccatg ccaaccggcg ctgcgtcgat cagtccggcg ccgctccagt
     1021 cggggaccag tttcctgccg tccacggtca gcacaacctg gtcggatgca gccgggatca
     1081 aggggatcca gtaccggacc catttcaacg ggaccgccag cgcctggcag acccagctcg
     1141 gcacaacgac gatcccggtc aaccagaccg agacctacgg atacctgacc cagaagacct
     1201 tctcggtcac cgtcactacc ccgggcacga cctatgtcga gagccgggcg atggacgtga
     1261 acggcaacct cagcgcctcg aaggtcaccg aggtcaacta cgacccgaac gcgatcgccg
     1321 cgccacagat cacttcgacc ccgccctcga tcaccgcctc gaacgcggcc agcttctcgt
     1381 ttaccggcgc gatcagcggc accagctacg aatgctcgct tgacgggggc gcgttcagcg
     1441 actgctcgcc accgaagtcc tactcgtcga tcgctgacgg ccagcacgag ttccgggtcc
     1501 gcctgaagtc agcggtcgcc tccgggccgg ccgtcagcta ccagtggcag atcgactcct
     1561 cggcccctcc cgcgccgacg attcacaccg gtcctgccgc caacaccaaa ctgacggtcg
     1621 ccgatttcac cttctcgggc accgaagccg ccctcgagtg ctcgctcgac ggcgctactt
     1681 tcgcttcctg cacctcgccc cggaacgtct tcgtcccgac tgacggggtc caccggttcg
     1741 aagtcaggca gaagacgatc acgggagtcc cgggaacctc cgccgtttac acatggacgg
     1801 tcgacaacac gaagcccccc gccccggttt tcggcgaagg tccctcctcc ccgaccaact
     1861 cgacctccgc ttcgatccag ttcactggcg aaccgggcgg gacttacagc tgccagctca
     1921 gtggcccggg cgggctccag atcaacaact gcaccagccc ccgcaacctg gccggactgg
     1981 cgcagggcga gtacacgctc tcggtttccc agaaggacca gctcggcaat cagggcccgg
     2041 ccgccagcta ctcctggacg gttgacctga ccgccccggt ggcgccgcag gtcggttccg
     2101 gaccaccggc ccggaccgca agccgcgacg ccacggttac cttcaccggg gaggccgggg
     2161 gtcacttcga gggcgtacag ggcggcgtac ttcgccggca gggt
//
LOCUS       DAOQFI010000315         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28747_length_2200_cov_2.778555, whole genome shotgun sequence.
ACCESSION   DAOQFI010000315 DAOQFI010000000
VERSION     DAOQFI010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28747_length_2200_cov_2.778555"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLE93_06200"
     CDS             <1..505
                     /locus_tag="PLE93_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNH86672.1"
                     /translation="NIQFAIGQGYFQASPLQLATAYAALGNGGTVLQPQLMLQTEDAA
                     GRVKSEAEPVVTGEIPIDPAARQVIMDGLHDAAQAPEGTSYKVFGGFPVKVAGKTGTA
                     ERTDEIDQAWYAVLAPYPDPEIAVVVTVEQGGFGADTAAPVALQILQAYFDKQAHAVS
                     GGVGSVE"
     gene            505..1689
                     /locus_tag="PLE93_06205"
     CDS             505..1689
                     /locus_tag="PLE93_06205"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNH86673.1"
                     /translation="MESSRLPRRTPKQSVNPLERAGLAGMDGWLAFSALGLLACSLFT
                     LYEASGSQIVGAPEQYVQRQALYGLVGIIGMIAVSRIDYSRFRELRVGIYTFLCASIF
                     AVFTFGVAARGSTNSLELPFFTFQPAELGKLLLVLALAGFVIDGARRGSEGQRTIRIL
                     ALGLVPTGLVLLQDLGTSLVFAAITFAIMVAAGVRWSHLAAIGAVLAVFVTVVLVALP
                     AAGMPVLKEYQQERLTSFLSPSSDIGDAGYQQNQAKVGIGAGGTTGRGDQATQSRFGF
                     VPERHTDFIFAVVAERYGFVGAALVLSLFALLFWRALRLMTLSKNFYGTLVAAGIAAA
                     IIFQVFVNVGMNLGIMPITGIPLPLMSYGGSAVIATFLMIGVLQSIHIESRAAERGPW
                     ID"
     gene            1733..>2200
                     /locus_tag="PLE93_06210"
     CDS             1733..>2200
                     /locus_tag="PLE93_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HNH86674.1"
                     /translation="MKKTIVVSADRGETRVAVLEAKTVKSKREVAEVYIERRGGRSLV
                     GNIYLGKVDNVLPGMEAAFVDIGLERNGFLHVDEILLPDGQQAPRRGRGQGRRIDELI
                     KPKQEILVQVIKDPIKSKGARLSMNVTIAGRYLVYAPNGTGVGVSRRLADKERD"
BASE COUNT          374 a          715 c          701 g          410 t
ORIGIN      
        1 gaacatccag ttcgcgatcg gccagggcta cttccaggcc agtccgctgc agctggcgac
       61 ggcctacgcc gcgctcggca acggcggcac cgttctccag ccgcagctga tgctgcagac
      121 cgaggacgca gcgggccggg tcaagtccga ggccgaaccg gttgtgaccg gggaaatccc
      181 gattgatccg gcggcccggc aggtgatcat ggacggactt cacgacgccg cgcaggcacc
      241 cgaaggcacc tcgtacaagg ttttcggcgg cttcccggtc aaggtcgcgg gcaagaccgg
      301 caccgcggag cgcaccgatg aaatcgacca ggcctggtac gcggtgctgg ccccgtatcc
      361 ggatccggaa atcgccgttg tcgtcacggt cgagcaaggc gggttcggtg ccgacaccgc
      421 cgcgccggtc gccctccaga tcctccaggc ctatttcgac aaacaggccc atgcggtctc
      481 gggcggggtc gggagcgtgg agtgatggaa tcctcgcggc tgccgcgccg gaccccgaag
      541 cagtcagtca acccgctgga gcgcgccggc ctggccggga tggacggatg gcttgccttt
      601 tcggctctgg gcctgctcgc ctgctcgctt ttcacgctat acgaggcttc cggctcgcag
      661 atcgtcgggg cacccgagca gtacgtccag cggcaggctc tctatggttt ggtcggaatc
      721 atcggaatga ttgccgtctc ccgcatcgac tattcccgtt tccgggaact acgtgttggc
      781 atctacacgt tcctgtgcgc ctcgatcttc gcggtgttca cattcggcgt ggccgcacgc
      841 gggtcgacga actcacttga acttcctttc ttcaccttcc agccggccga gctgggcaag
      901 ctgcttctcg tgcttgccct ggccggattc gtgatcgacg gggcccgtcg tggctccgaa
      961 ggtcaaagga cgatccgcat actcgccctc ggactcgtgc cgaccggcct ggtcctgctt
     1021 caggacctgg gtacgtcgct cgtcttcgcg gcgattacct tcgcgatcat ggtcgcggcc
     1081 ggcgttcgct ggtctcacct ggccgcgatc ggggccgtcc tcgcagtttt tgtcaccgtc
     1141 gtgctggtcg cccttccggc ggccgggatg ccggtgctga aggagtatca acaggaacga
     1201 cttacctcgt tcctcagccc gtcgtccgat atcggtgacg cgggctacca gcagaatcag
     1261 gccaaggtcg gaatcggagc cggtggaacc accggccgcg gcgaccaggc cacccagtcc
     1321 cgcttcggat tcgttcccga acggcacacg gacttcattt ttgccgtggt tgccgagcgt
     1381 tacggattcg ttggcgcggc gctggtctta tccctttttg ccctgctctt ctggcgggcg
     1441 ctgcgtctga tgacgctctc gaagaatttc tacggaaccc tggtcgcggc cggaatcgct
     1501 gccgcgatca tctttcaggt cttcgtaaac gtgggaatga acctcggcat catgccgatc
     1561 accggcatcc ccttgccgct gatgagttac ggcgggtcgg cggtaatcgc caccttcctg
     1621 atgatcgggg tcctgcagag cattcacatc gagtcgcgcg ccgcagagag gggtccgtgg
     1681 atcgactgag aatccacagg gcagccaatc tgaacggaaa acgacttaga aaatgaaaaa
     1741 gacaattgtt gtgtcggccg accgcggcga aacccgcgtt gccgtgctcg aggccaagac
     1801 ggtcaaatca aagcgcgaag tggccgaggt ctacatcgaa cgccggggcg gccgctccct
     1861 ggtcgggaac atctacctgg gcaaggtcga caacgtcctg cccgggatgg aagcggcctt
     1921 cgttgacatc ggcctggagc gaaacggctt cctgcatgtc gacgagatcc tgctccccga
     1981 cggccagcag gcaccccgcc gcggccgcgg ccagggccgc cggatcgacg agctgatcaa
     2041 gcccaaacag gagatcctgg tccaggtgat caaggatccg atcaagtcga agggcgcccg
     2101 cctttcgatg aacgtgacca tcgccggccg ctacctggtc tacgcgccga acggcaccgg
     2161 ggtgggggtg agccgccggc tcgccgacaa ggaacgcgac
//
LOCUS       DAOQFI010000316         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28966_length_2189_cov_3.669166, whole genome shotgun sequence.
ACCESSION   DAOQFI010000316 DAOQFI010000000
VERSION     DAOQFI010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28966_length_2189_cov_3.669166"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLE93_06215"
     CDS             complement(<1..491)
                     /locus_tag="PLE93_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86675.1"
                     /translation="MFVRWDGQRVTADGRPAKAGPAMALPGMEDAGLGPVRTFSAPEA
                     MGIRFHEVRAKSALNRVPEGPYGFGWTVNPYRGCSHACVYCFARPTHTYLGFNAGRDF
                     DREIVVKVNAPEVVRAELARPSWKRELVALGTNTDPYQWVESRYRLTRGVLEALDEAD
                     TPVS"
     gene            572..1663
                     /locus_tag="PLE93_06220"
     CDS             572..1663
                     /locus_tag="PLE93_06220"
                     /inference="COORDINATES: protein motif:HMM:NF022470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HNH86676.1"
                     /translation="MSDSYDFAVRRDDMRETSVLETPGADREIDEGQILLEVDHFSLT
                     ANNVTYGAMGEAMNYWDFYPAEDDSLGRIPAWGYADVVVSKAEGIEEGARVFGYLPMS
                     THAVLEPGRIRESGFTDITGFRSGLPAVYNEYRLITGEKDDERERLVAIFMPLYGTSW
                     LLADWLNEVDFAGASQLVASSASSKTALGLGHALQVDFDNPIETVGLTSPGNLEFTTG
                     TGHWDAVVTYDDLDSIDADTPTIYVDFGGNAKLRHRIHSHFGDRLVASVAVGIADWES
                     MVPEEGDSLPGPQPQFFFAPDRVVKRNADWGQDGLGERLAKSQDRFTDRSREWMEVQT
                     GSGPDAIRESLLALMDGKVPPDRGISVNP"
     gene            complement(1715..>2189)
                     /locus_tag="PLE93_06225"
     CDS             complement(1715..>2189)
                     /locus_tag="PLE93_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNH86677.1"
                     /translation="TCAFNDFALVRINPADVAGVNPSVPGYGGPTGLGAPGGLGSTVY
                     SWGNSSLRQGIRQLSPKQGIVLSNDASGWSHNVYTLTPGIPGDSGSGFLNNSGQAFGT
                     LSTLQLAPTVGANGVGDLAKELTWLKANEPAFAGIQLVNGTEPFKPNLVGAILGG"
BASE COUNT          370 a          698 c          734 g          387 t
ORIGIN      
        1 gagaccgggg tgtcggcctc atcgagcgct tcgagcacgc cgcgggtcag ccggtacctg
       61 ctttcgaccc attggtaggg gtcggtgttg gtgccgagcg cgaccagctc gcgcttccat
      121 gaaggtcgcg cgagttcggc ccggaccacc tccggggcat tgaccttgac cacgatctcc
      181 cgatcgaagt cccggcccgc gttgaagccg aggtaagtgt gggtggggcg ggcgaagcag
      241 tagacgcagg cgtggctgca gccgcggtac gggttgactg tccagccgaa gccgtaaggc
      301 ccttccggca cccggttgag tgccgacttg gcccggactt cgtggaacct gatccccatc
      361 gcttccggag ccgagaaggt gcgtaccgga ccgagcccgg cgtcttccat ccccggcaag
      421 gccattgccg ggccggcctt cgccgggcgc ccgtcggcgg ttacccgttg cccgtcccat
      481 cgcacgaaca tatgttcgca tatccggcga cccggttcgc tgatgcaaca ccctctgcaa
      541 gtgtttcagt caaatcgata gtctcaccga catgtccgac tcttatgact ttgcagtccg
      601 ccgcgacgac atgcgggaaa cctcggttct cgagactccg ggagccgacc gggagatcga
      661 cgaaggccag atcctgctcg aggtcgacca cttctcgctg accgccaaca acgtcaccta
      721 cggtgcgatg ggcgaggcga tgaactactg ggatttctat ccggccgagg atgattcact
      781 gggccggatc ccggcctggg gatatgccga cgtggtggtc tcgaaggccg agggaatcga
      841 ggaaggtgcc cgggtcttcg ggtacctgcc gatgtcgact cacgccgtcc tcgagccggg
      901 ccggatccgg gagtcgggtt tcaccgatat caccggtttc cgctccgggc tcccggcggt
      961 ctacaacgaa taccgcctga tcaccgggga gaaggacgat gaacgggagc ggctggttgc
     1021 gatcttcatg cctctctacg gaacctcgtg gcttctggcc gactggctga atgaagtcga
     1081 tttcgccggc gcctcccagc tggttgcctc cagcgcttcg agcaagacag cgctcggtct
     1141 cggacacgcg ctgcaggtcg atttcgacaa tccgatcgag acggtgggcc tgacctcgcc
     1201 cggcaatctc gagttcacca cgggcaccgg gcactgggat gcggtcgtca cctatgacga
     1261 cctcgacagc atcgacgcgg acacgccgac catctacgtc gatttcggcg gcaacgcgaa
     1321 gctcaggcac cggattcaca gtcacttcgg tgaccggctc gtggccagcg ttgcggtcgg
     1381 gatcgctgac tgggaatcga tggttccgga agaaggcgat tctctgcccg ggccccagcc
     1441 gcagttcttc ttcgcgcccg accgggtcgt caagcgcaac gccgactggg ggcaggacgg
     1501 cctcggcgag cgcctggcca aaagccagga ccgtttcacc gaccgcagcc gcgagtggat
     1561 ggaagttcag acgggaagcg gaccggatgc gatccgggag tccctgctcg ccctgatgga
     1621 cggcaaggta cccccggatc gaggcatctc ggtcaatccc tagaacccgt ggccaagacg
     1681 tcccgaggac ggagtcttgg ccacgggttc cagctcagcc gccgaggatc gcgccgacca
     1741 ggttcggctt gaagggctcg gttccgttga ccagctgaat gccggcgaag gccggctcgt
     1801 ttgccttgag ccaggtcagt tccttcgcga ggtcaccgac tccgttcgcg cccaccgtgg
     1861 gtgcgagctg cagggtgctg agcgtgccga aggcctgacc gctgttgttc aggaagccgc
     1921 tgccggagtc accggggatg ccgggggtca gggtgtagac gttgtggctc cagccgctgg
     1981 cgtcattgct gagaacgatg ccctgcttcg ggctgagctg gcggatgccc tggcggagcg
     2041 acgagttgcc ccaggagtag acggtcgagc caagtccgcc cggcgcacca agcccggtcg
     2101 gtccgccgta gccggggacc gaggggttga cgccggcgac gtcggccgga ttgatcctga
     2161 ccagcgcgaa gtcattgaag gcgcaggtc
//
LOCUS       DAOQFI010000317         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_28968_length_2189_cov_3.446579, whole genome shotgun sequence.
ACCESSION   DAOQFI010000317 DAOQFI010000000
VERSION     DAOQFI010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28968_length_2189_cov_3.446579"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLE93_06230"
     CDS             <1..222
                     /locus_tag="PLE93_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNH86678.1"
                     /translation="GATLAVALRQTYGRTGLAVATSGLTAIAGFAALGSSDVAMLRDF
                     GLIAVIDLGVALAGVALVLPAMLVWLERR"
     gene            219..884
                     /locus_tag="PLE93_06235"
     CDS             219..884
                     /locus_tag="PLE93_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH86679.1"
                     /translation="MKEAGKKPFSYMTLVGVIFLVVVLVALFNMLKTTDSGTVGIGEV
                     GIGDRAAPFAVPLAMSGLDGDANIDPDEACSVEGADVLRICDYFERPLVMSFWFTKGA
                     SSCIDQQDVFDEVAAKYRRQAGFVSINVRDDRDRVRELIGEHGWKVPVGYDRDGAVSN
                     IYRVGGCPTFLFFRKGGVLEDAEIGENDAGQLGAQVRSLIDGESKATQQESASDPESG
                     PTG"
     gene            910..1368
                     /locus_tag="PLE93_06240"
     CDS             910..1368
                     /locus_tag="PLE93_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86680.1"
                     /translation="MESTTRQSGPAERRESRTRFGKWADRLEETADERPPAPWGSFPI
                     GAVSVFVGLVLAVIGLINANPVQLAIGVGLGMLGGLELAIREHFTGFRSHTTLLAGFV
                     FVVAVGATFYAAHWILWQSLLLGAALFGATFWLLRKKFEKASGGLSYKLR"
     gene            complement(1471..1809)
                     /locus_tag="PLE93_06245"
     CDS             complement(1471..1809)
                     /locus_tag="PLE93_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86681.1"
                     /translation="MEVAATADSKSIASRPMPQHLQALERANRVRLARAALKRSIASG
                     EVSVTKVITDCPWQTETMTLSELLRAQSRWGRTRTRKLLASVGLSENKRLDTLTERQR
                     MLLVSQLRPH"
BASE COUNT          344 a          674 c          772 g          399 t
ORIGIN      
        1 ggcgcgaccc tggcggttgc gctccgccag acctatggcc ggaccgggct ggccgtcgcg
       61 acctccggcc tcacggcgat cgccggattc gcggcgctcg gatccagcga cgtggcgatg
      121 cttcgggact tcggcctgat cgcggtgatc gatctcggcg ttgcgctggc cggagtggcg
      181 ctggtcctgc cggcgatgct ggtctggctg gagcgccgtt gaaggaagcc gggaagaagc
      241 ccttcagcta catgaccctg gtcggggtca tcttcctggt ggtcgtcctg gtcgcgctct
      301 tcaacatgtt gaaaaccacc gactcgggca cggtcgggat cggggaggtg gggatcggtg
      361 accgggcggc tcccttcgcc gtgccgctgg cgatgtccgg actcgacggg gacgccaaca
      421 tcgacccgga cgaggcctgc tcggttgaag gtgccgacgt gctgaggatc tgcgactact
      481 tcgagcggcc gctggtcatg agcttctggt tcaccaaggg cgcttcgagc tgcattgacc
      541 agcaggacgt attcgacgag gtcgccgcga agtaccgccg gcaggcgggc tttgtttcga
      601 tcaacgtccg cgacgaccgt gaccgggtcc gggaactgat cggcgagcac ggctggaagg
      661 ttccggtcgg gtacgaccgg gatggcgcgg tttccaacat ctaccgggtc ggcggctgcc
      721 cgacctttct cttcttccgc aagggcgggg tgctcgaaga cgccgagatc ggcgagaacg
      781 acgccggaca gctgggggcg caggtacgct ccctgatcga tggcgagtcg aaggcaacgc
      841 agcaggaaag cgcgtcggac ccggaatccg gtcccaccgg ctgaggcggc ccgggccgac
      901 gccgggtccg tggaaagcac gacgcggcag tccgggcctg ccgagcgccg cgagtcgcgc
      961 acgaggttcg gcaagtgggc cgaccggctg gaggaaaccg cggacgagcg gccgcccgca
     1021 ccctggggca gctttccgat cggcgcggtc tcggtcttcg tcgggctcgt gctcgcagtg
     1081 atcggcctga tcaacgccaa cccggttcag cttgcgatcg gagtcggcct cggcatgctg
     1141 ggcgggctcg aactggcgat ccgcgagcac ttcacggggt tccggtcaca tacgacgctg
     1201 ctggcgggtt tcgtcttcgt ggtcgcggtc ggggcgacct tctacgcggc ccactggatc
     1261 ctctggcagt cgctcctgct cggcgcggca ctgttcggag ccaccttctg gctgctcagg
     1321 aagaagttcg aaaaggcctc cggaggcctc agctacaagc tccggtgact caactgcagt
     1381 cgggcggggt ccaacccgcc ctcccagtag aactcgcccc ttcccccgaa aaaaagaatg
     1441 gagggaaggg gctcagtctg gtggtgcctc tcagtggggg cggagctggc tgacgagcag
     1501 catgcgctgg cgctcggtca gggtgtcaag gcgcttgttc tcgctcaggc cgaccgaggc
     1561 caggagcttt ctcgtccggg tccggcccca gcgggactgg gcgcggagca gttccgagag
     1621 ggtcatcgtc tcggtctgcc agggacagtc ggtgatcact ttggtaacgg aaacttcacc
     1681 gctggcgatt gatcgcttca gggctgcgcg agcaagtcga acccgattgg cccgttcgag
     1741 agcctgcaga tgctggggca tcggacggct ggcaatcgac ttagaatcgg cggtggcggc
     1801 aacttccacg ggcggtcctc ccttcggggg attggtcttg ttgactggat ggcatggggc
     1861 gacaggccag ggatcctctt tcccgggtgg cgcttctccc ccgcctggtg ccgggctttc
     1921 cgtccgactc aggcgataaa ggcaacatag acaagttggc cggaccgggc aatggtcagg
     1981 gccgcgaact ttcgctaaga gcaggttttt ctttttcgcc gaattccctt gtcggggacc
     2041 tgcccggaat gtatggtcga ggcacggtct gaccgaacac tgttcggtcg gcgcaaggtt
     2101 caagtggcct ccagtccgac ccggccacct acagacggga gagagagtgt ccaagcacgt
     2161 gaagcttcta ctcggtctcg gcgccctgg
//
LOCUS       DAOQFI010000318         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29010_length_2188_cov_2.838725, whole genome shotgun sequence.
ACCESSION   DAOQFI010000318 DAOQFI010000000
VERSION     DAOQFI010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29010_length_2188_cov_2.838725"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PLE93_06250"
     CDS             <1..1172
                     /locus_tag="PLE93_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNH86682.1"
                     /translation="AQHAQDLFALAEPGNIYTRIMNPTWNVLEERMAALEGGVAALAL
                     ASGQAAVTYSVLNVCRTGDNIVALSTLYGGTYNLFAHTLPQYGIEVRFVDPDKPEELE
                     KHVDDKTRLVFGETIGNPRLNVMDVEAWANAAHAQGLPLVVDNTVATPVLTRVFELGA
                     DIAVHSATKFIGGHGTTIGGVIVDSGNFDWVANADRFPGLTQPDPSYHGVVWADALGP
                     AAYIGRVRTVLLRNTGAALSPFNAFLLLQGVETLPLRMERHSANALAVAEHLSDSENV
                     LWVNYPGLPGFSHHETASKVLTGGYGALVTFGIKGGRDAGRKFIDSLELFSHLANIGD
                     AKSLAIHPATTTHSQLNDEELEDSGVTADTVRLSVGIETIGDILADIDQALAKAS"
     gene            1173..>2188
                     /locus_tag="PLE93_06255"
     CDS             1173..>2188
                     /locus_tag="PLE93_06255"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNH86683.1"
                     /translation="MPDASSGIGIVETQRAVVFSGDRPLTLASGAILPEVEVAYETYG
                     ELNPDRSNAVFICHALSGDAHAAGLHEGDDPSRPGWWDNIVGPGRPLDTDRFFVISAN
                     VLGGCKGTTGPSSIDPATGKPYGLRFPLVQVRDLVEVHRALLRELGIDHLLAAIGGSL
                     GGMQALQWAIDHPDEVDGAAIIAASARLSAQNIAFSAVAREAIMRDPDFAGGDYYERE
                     RRPDPGLALARMIGHITYLSEESMREKFGRRIQDAEVPRYGFDVDFQVESYLHYQGQS
                     FVERFDANTYLYMGRTMDYFDPFEDPVLVQNQISQGETSFLVISFDSDWRFSTEHARV
                     LASGL"
BASE COUNT          354 a          788 c          689 g          357 t
ORIGIN      
        1 acgcccagca cgcgcaggac cttttcgccc tggccgagcc gggcaacatc tacacgcgga
       61 tcatgaatcc gacctggaac gtgctcgagg agcggatggc cgcgctcgaa ggcggtgtcg
      121 ccgccctcgc tctggcctcc ggccaggccg cagtcaccta ctcggtcctg aacgtctgcc
      181 ggaccggcga caacatcgtc gccctttcga ccctctacgg cggcacctac aacctcttcg
      241 cccacacgct cccccagtac gggatcgagg tcaggttcgt cgatccggac aagcccgagg
      301 agcttgagaa gcacgttgac gacaagaccc ggctggtctt cggcgagacg atcggcaatc
      361 ccaggctgaa cgtgatggac gtcgaggcct gggccaacgc tgcccacgcc cagggcctgc
      421 cgctggtggt cgacaacacc gtcgcgacgc cggttctgac cagggtcttc gagctggggg
      481 ccgacatcgc ggtccactcc gcgaccaagt tcatcggtgg tcacggaacc acgatcggcg
      541 gcgtgatcgt ggactccggc aacttcgact gggtcgccaa cgccgatcgc tttcccggcc
      601 tgacccagcc cgacccctcc taccacggcg tggtctgggc ggacgcgctc ggcccggcgg
      661 cctacatcgg ccgggtccgg accgtgctgc tgaggaacac cggcgcggcc ctttcgccgt
      721 tcaacgcctt cctcctgctc cagggcgtcg agaccctgcc gctgaggatg gagcgccaca
      781 gcgcgaacgc cctcgcggtg gccgagcacc tgagtgacag cgagaacgtg ctctgggtga
      841 actacccggg cctgcccggc ttcagccacc acgaaacggc gagcaaggtg ctgaccggtg
      901 gctacggcgc cctggtcacc ttcgggatca agggcggccg cgatgccggt cgcaagttca
      961 tcgactcgct ggagctgttc tcgcacctgg ccaacatcgg tgacgccaag tcactggcga
     1021 tccacccggc gaccacgacc cactcgcagc tgaatgacga agagctcgag gattccggcg
     1081 tgaccgcgga cacggtgcgg ctctcggtcg ggatcgagac gatcggggac atcctcgccg
     1141 acatcgacca ggccctggcc aaggcaagct agttgccgga cgcttccagc ggcatcggca
     1201 ttgtcgagac gcagcgggcg gtcgtctttt ccggcgaccg cccgctgacc ctcgcctccg
     1261 gggcgatcct gccggaagtc gaggtcgctt acgagaccta cggcgagctc aaccccgacc
     1321 gctcgaacgc ggtcttcatc tgccacgcac tttccggcga cgcccacgcg gcggggctcc
     1381 acgagggtga cgatccttcc cggcccggct ggtgggacaa catcgtcggc ccgggccgcc
     1441 cgcttgacac cgaccgtttc ttcgtgatca gcgcgaacgt gctcggcggc tgcaagggca
     1501 cgaccggccc ctcgtcgatc gaccctgcca ccggcaagcc gtacggcctg cgcttcccgc
     1561 tggtccaggt gcgtgatctg gtcgaggtcc accgggccct gctgcgggaa ctggggatcg
     1621 atcatctcct cgcggcgatc ggcggctcgc tcggtggcat gcaggccctg cagtgggcga
     1681 tcgaccatcc cgatgaagtg gacggcgccg cgatcatcgc cgcctcggcc cggctttcgg
     1741 cccagaacat cgccttctcc gcggtcgcgc gggaggcgat catgcgcgat ccggacttcg
     1801 ccggcggcga ttactacgaa cgcgaacgcc ggcccgaccc cggccttgcc ctggcccgga
     1861 tgatcggcca catcacctac ctctcggagg agtcgatgcg ggagaagttc ggccggcgga
     1921 tccaagatgc cgaggtgccg cgttacggct tcgacgtcga cttccaggtc gagagctacc
     1981 ttcactacca gggccagtcc ttcgtcgagc gcttcgacgc gaacacctac ctctacatgg
     2041 gccggaccat ggactacttc gaccccttcg aagacccggt cctggtccag aaccagatca
     2101 gccagggcga gacttccttc ctcgtgatct ccttcgattc cgactggcgc ttctcgaccg
     2161 agcacgcccg ggtgctggct tccgggct
//
LOCUS       DAOQFI010000319         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29135_length_2183_cov_2.491541, whole genome shotgun sequence.
ACCESSION   DAOQFI010000319 DAOQFI010000000
VERSION     DAOQFI010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29135_length_2183_cov_2.491541"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..1230
                     /locus_tag="PLE93_06260"
     CDS             34..1230
                     /locus_tag="PLE93_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HNH86684.1"
                     /translation="METELKPGAGRNLALATVGFALCFAAWGFLAPLAPSFKDPPLNL
                     SSLQTSIAIAIPVLLGSLLRIPLGILTDRIGGRKVFTGMLIYSAAAAALVGFAAGSSS
                     YAAILGAGFLLGAAGASFAVGIPFVARWYPPQKQGFALGIYGMGNIGTAVAAFSIPAI
                     RDATSEGVAGLVFAAVIGVYALIWMSMAREAPVEKAPPTRYREVLGAGWKLWRLALFY
                     FVTFGGFVAMALYLPKLLVDWYDLSLTDAGLRAAGFTLLATAIRPVGGMLSDRIGGNR
                     VLAVAFLGIGIDAVGLSWQASVPTMWLTTVFCLTMALFLGLGNGAVFKLVPLTFPNAT
                     GAATGIVGAVGGLGGFFPPLFLGIVKDATGDFVLAFVMLVAFAWLCAGLAFEGRVPDS
                     GTNGPD"
     gene            complement(1313..1945)
                     /locus_tag="PLE93_06265"
     CDS             complement(1313..1945)
                     /locus_tag="PLE93_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNH86685.1"
                     /translation="MSQIKMVISDFGGVLTTPLVEAFASVQDRVGIPPKTFGEALRAL
                     NEREGTNPLFELECGRITEKEFQVQLADGLEPLLGHRPEIEDFGLLLFEALDPNPGMI
                     DLIREVRRDGIRTSLLTNNVKEWEVKWRSMMPIDELFETVVDSAFVGCRKPDPRIYNL
                     TLERVGLDPEECIFIDDMKINIDAANELGLHGVHFRETAQVRAEVHDLLA"
     gene            complement(1942..>2183)
                     /locus_tag="PLE93_06270"
     CDS             complement(1942..>2183)
                     /locus_tag="PLE93_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86686.1"
                     /translation="EVLLSTEYDTAGRQRRATLELWPATDEVAALHGAGSVIAGCTAK
                     VGGSTINTALFRWSLDGHLGLGRYEITRPAAVAVA"
BASE COUNT          319 a          753 c          693 g          418 t
ORIGIN      
        1 ctctttccac taacacacca acaggagaat ccgatggaaa ctgaactcaa acccggtgca
       61 gggcgcaacc tggctctcgc cacggttgga ttcgcgctct gcttcgcggc ctgggggttc
      121 cttgccccgc tcgcgccgtc gttcaaggat ccgccgctca acctttccag cctgcagacc
      181 tcgatcgcga tcgccatccc cgtcctgctc ggctcgctgc tccgcatccc gctcggcatc
      241 ctcaccgacc ggatcggtgg ccgcaaggtc ttcaccggga tgctgatcta ctcggccgca
      301 gccgcggcac tggtcggatt cgctgccggg tcatcgtcct acgcggccat tctcggcgcc
      361 ggcttcctgc tcggcgccgc cggagcatcc ttcgcggtcg gcattccgtt cgtcgcccgc
      421 tggtacccgc cgcagaagca gggcttcgcg ctcggcatct acggcatggg caacatcggc
      481 accgcggttg ccgccttctc gattccggcg atccgcgacg ccaccagtga aggtgtggcg
      541 ggactcgtct tcgccgcagt gatcggcgtc tacgcgctga tctggatgtc gatggcccgg
      601 gaggcaccgg tcgaaaaggc gccgccgacc cgctaccgcg aggtgctcgg cgccggctgg
      661 aaactctggc gactggcgct cttttacttc gtgaccttcg gtggattcgt cgccatggcg
      721 ctatacctgc cgaagctgct ggtcgactgg tatgaccttt cgctcaccga cgccgggttg
      781 cgcgctgccg gcttcaccct gctggcaacc gcgatcaggc cggtcggcgg catgctctcg
      841 gaccggatcg gtggcaaccg cgtactggcg gtcgccttcc tcgggatcgg catcgacgcg
      901 gtcggcctct cctggcaggc ctcggttccg acgatgtggc tgaccaccgt cttctgcctg
      961 accatggccc tcttcctcgg tctcgggaac ggcgccgtgt tcaagctggt gccgctcact
     1021 ttcccgaacg caaccggtgc cgcaaccggc atcgtcggcg ccgtgggtgg cctcggcggc
     1081 ttcttcccgc ccctgttcct gggaatcgtg aaggacgcaa cgggtgactt cgtgctcgcc
     1141 ttcgtgatgc tggtcgcctt cgcctggctg tgtgccggcc tggccttcga gggccgggtt
     1201 cccgactcgg ggacgaacgg cccggactag tctgagccac ttcggtacgg cgcccctccc
     1261 gacctccgag cgggaggggc tccgcccgaa gtaaagccag gcgaaaccct gtttacgcca
     1321 gcaggtcgtg cacttcggcc cggacctgtg cggtttcgcg gaagtgaacc ccgtggagcc
     1381 cgagttcgtt cgcggcatcg atgttgatct tcatgtcgtc gatgaagatg cattcctccg
     1441 ggtcgaggcc gacccgttcc agggtcaggt tgtagatccg cggatccggc ttgcggcagc
     1501 cgacgaaagc cgagtcgacc acggtctcga agagctcgtc tatcggcatc atcgagcgcc
     1561 atttgacttc ccactccttg acgttgttgg tcagaagtga agtgcggatg ccgtcccggc
     1621 ggacctcccg gatcaggtcg atcatgcccg ggttcgggtc aagggcttcg aagagcagca
     1681 ggccgaagtc ctcgatttcc ggccggtggc cgagcaacgg ctcgaggcca tccgccagct
     1741 ggacctgaaa ttccttttcg gtgatccggc cacactcgag ctcgaagagc ggattggtgc
     1801 cctccctctc gttcagcgcc cgcagcgcct cgccgaaggt cttgggcggg atcccgaccc
     1861 gatcctgcac cgaagcgaat gcctcgacca gcggggtcgt cagcaccccg ccgaaatccg
     1921 agatgaccat cttgatctgg ctcatgcgac cgctaccgcc gcgggacggg tgatctcgta
     1981 acggccgagg ccgaggtggc catcgagcga ccagcggaag agggcggtgt tgatcgtcga
     2041 gcccccgacc ttcgcggtgc agccggcgat caccgatccg gcaccgtgaa gcgcggccac
     2101 ctcgtcggtg gccggccaga gctcgagggt ggcccgacgc tggcgcccgg ccgtgtcgta
     2161 ctcggtcgaa agcaggactt cat
//
LOCUS       DAOQFI010000320         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29188_length_2181_cov_2.219661, whole genome shotgun sequence.
ACCESSION   DAOQFI010000320 DAOQFI010000000
VERSION     DAOQFI010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29188_length_2181_cov_2.219661"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PLE93_06275"
     CDS             complement(<1..888)
                     /locus_tag="PLE93_06275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH86687.1"
                     /translation="MAPGKRVTDPLQWGYSLSNVFELLSGCLEAIETKTVLEIGSYKG
                     ELTVELLEWAQTRGVKVAGVDPLPPDELEALAKAHPELELLRQTGQEVLREREFDDAI
                     IIDGDHNYYTLSEELKIIGERAPGAGMPLLMFHDVCWPHARRDTYYEPDRIPEDQRQP
                     LVHNEGIAPGIKGTAEWGLPFVWAAAEEGGEKNGTMTAIEDFLAERPGIQLAVVPAFF
                     GFGVMWHRDAPWASAVERVIAPYDRHPVLERMEGNRVEHLVAGQARARQLVKLEEKVA
                     KQEDLLRRLLGSSAFTAAEK"
     gene            919..1518
                     /locus_tag="PLE93_06280"
     CDS             919..1518
                     /locus_tag="PLE93_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86688.1"
                     /translation="MDDLGQHNDAASLMADLRSRHPRFTEAVLADTVVTAQHRGERYE
                     FRGKGDALVAALRLAWVSDAFLAQVLYRAKAACQRREVRVLPRLFHRLSMMLAQVSIG
                     DPVVMHPGVYIVHGQVVLDGIVEIHPGVVISPWVTIGLRAGNVVGPTVREQVNIGTGA
                     KLIGPIDIGAASSIGANAVVVDDVPAGTTAVGVPASQPG"
     gene            1631..>2181
                     /locus_tag="PLE93_06285"
     CDS             1631..>2181
                     /locus_tag="PLE93_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86689.1"
                     /translation="MGIFRRKDRGAAAVAEAEPELNPVVEEARALEADGRIFDGIDLL
                     TRTNRGSGNVEIEREIIAMRHRAGAEMVEAGDQHPVHPEPADYDFRLEDKVPTVTPAE
                     LTPEILRAAILKYGCLKVTGVMDEATAKSLADDIDHAFEIRQRLSDAKEVDPTRIYEE
                     FAPNEPYQVQYVERHWVALAGGVL"
BASE COUNT          359 a          723 c          722 g          377 t
ORIGIN      
        1 cttctcggcc gcggtgaagg cgctcgatcc gagcagccgc cgcagcagat cctcctgttt
       61 ggcgaccttc tcttccagct tgaccagctg gcgggcccgg gcctggccgg cgaccaggtg
      121 ctcgacccgg ttgccttcca tccgttcgag gaccgggtgg cggtcatagg gggcgatcac
      181 gcgctcgacc gcgctagccc agggagcatc ccggtgccac atcacgccga agccgaagaa
      241 ggccgggact accgccagct ggatgccggg ccgttcggcc aggaagtcct cgatcgcggt
      301 catcgtgccg ttcttctccc cgccttcctc ggcggcggcc cagacgaagg gcaggcccca
      361 ttcggcggtg cccttgatcc cgggggcgat gccctcgttg tgaaccagtg gctggcgctg
      421 atcctcgggg atccggtccg gctcgtagta ggtgtcgcgc cgggcgtgag gccagcagac
      481 atcgtggaac atcagcagcg gcatcccggc gccgggagcg cgctcgccga tgatcttcag
      541 ttcctcgctc agggtgtagt agttgtggtc gccgtcgatg atgatcgcgt catcgaattc
      601 ccgttcgcgc agcacctcct ggccggtctg gcgcaggagt tcaagctccg ggtgcgcctt
      661 cgccagggct tcgagctcgt ccggcggcag cgggtcgact cccgcgacct tgaccccgcg
      721 tgtctgcgcc cattcgagca gctcgaccgt caactcgccc ttgtaggagc cgatttcgag
      781 cacggttttc gtttcgatcg cttccaggca gcccgaaagc agctcgaaga cgttgctcag
      841 cgagtagccc cactggagcg gatcggtaac gcgttttccg ggagccatcg gcggcaaggg
      901 tatagattcg cccggcccat ggatgacctc ggtcagcaca atgacgccgc tagcctgatg
      961 gcggacctgc gctcccggca cccccggttc accgaagcgg tgctggccga cacggtggtg
     1021 accgcccagc accgggggga gcgatacgag ttccgcggca agggggatgc cctggtcgcg
     1081 gcccttcgcc tggcctgggt gagcgacgcc ttcctggccc aggttctcta ccgcgccaag
     1141 gccgcctgcc agcggcgcga agtccgggtt ttgccccggc tctttcaccg gctttcgatg
     1201 atgctggccc aggtttcgat cggcgacccg gtggtgatgc accccggcgt ctacatcgtc
     1261 cacggccagg tcgtgctcga cggcatcgtc gaaattcatc ccggcgtggt gatttcaccc
     1321 tgggtcacga tcggcctgcg ggccggcaac gtggtcggtc ccacagttcg cgagcaggtg
     1381 aacatcggta cgggcgccaa gctgatcgga ccaatcgaca tcggggctgc ctcatcgatc
     1441 ggagccaacg cggtcgtggt cgacgacgtc ccggccggga ccaccgcggt gggagttccg
     1501 gccagtcaac ccggttgaaa ccgggcatgc gggtaagtac tgaacctgtt ctgctttgca
     1561 ggccgggctg gtagcgtcca gcgggttcga gaaagcagaa ccgaactcac cagctaaagg
     1621 caggtttttg atgggcattt ttcgtcgcaa ggatcgtgga gcagccgcag tcgccgaggc
     1681 cgagccggaa ctcaatccgg tggtcgagga agcccgcgct ctcgaagctg acggaaggat
     1741 cttcgacggg atcgatctgc tgacccggac caatcgcgga agcggcaacg tcgagatcga
     1801 gcgggagatc atcgccatgc gccaccgggc cggcgcggaa atggtcgaag ccggggatca
     1861 gcacccggtc cacccggagc ccgccgacta cgattttcgc ctcgaagaca aggttccgac
     1921 cgtcaccccg gccgagctga ccccggagat tctccgcgcg gcaatcctca agtacggctg
     1981 cctgaaggta accggcgtga tggacgaagc gaccgcgaag tccctggccg atgacatcga
     2041 tcacgccttc gagatccgcc agcgcctgag cgacgccaag gaagtcgatc cgacccggat
     2101 ctacgaggag ttcgccccca acgagcccta ccaggtccag tacgtggagc ggcactgggt
     2161 cgccctggcc ggcggggtcc t
//
LOCUS       DAOQFI010000321         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29208_length_2180_cov_2.547294, whole genome shotgun sequence.
ACCESSION   DAOQFI010000321 DAOQFI010000000
VERSION     DAOQFI010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29208_length_2180_cov_2.547294"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLE93_06290"
     CDS             <1..605
                     /locus_tag="PLE93_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH86690.1"
                     /translation="PGTGTARIRSTGSRSLSTAEERREELITAAIPVFAEKGYHAAPT
                     TEIAKKAGISQAYLFRLFPTKEELFVAAVERSTGRMVETLAAVASEAEKNGIEPGDAM
                     GLAYGDLVSSDRELLLMQLHAQAVSPAIPAVREAMRKCFANLYALGSERTGGNGEEMK
                     NWFAHGMLINVMTAINAEEVDEPWAVALTDFEGDCFPRES"
     gene            819..>2180
                     /locus_tag="PLE93_06295"
     CDS             819..>2180
                     /locus_tag="PLE93_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNH86691.1"
                     /translation="MTASITSRPGESSGNRSAIWTLIITGLALFMTSLDNLVVGVALP
                     SIRADLGGTIESLEWTVNAYTLAFAVLLITGAALGDRFGRRRMFAIGVGLFTAASAAS
                     ALAPTLDALVAARALQGAGAAIVVPLTLTLVSGAFPAEKRGAALGIWGGISGLGVALG
                     PLFGGAVVEGISWHWIFWLNVPVGLALIPLALRRLPESTGPDKALDLPGLGLVGTGLF
                     GLTFGIIRGESLGWTSTTVLASLVTGALLIGAFIVWELRAREPMLPMRFFRSRGFSAT
                     NGLSFAMFFGTFGAIFLLSQFFQVAQGLGPLDAGIRTLPWTLMPMFVAPLAGFLSDRI
                     GTRPLIFTGLVLQAGGLAWITAISSPVTAFGDFVTPFMMAGTGMALVFPTAAATVVAS
                     VREEETGKASGATNAIREIGGVMGVAVLSSIFAANGGFTSPQAFTDGVVAAMPVAVAV
                     LAVG"
BASE COUNT          339 a          760 c          712 g          369 t
ORIGIN      
        1 ccccgggcac cgggactgcc aggatccgct cgacgggatc ccgctctctt tcgaccgctg
       61 aagaacgccg tgaggaactg attacggcgg cgattccggt ctttgccgag aagggttacc
      121 acgccgcccc gaccaccgag atcgcgaaga aggccggcat ttcgcaggcc taccttttcc
      181 ggctcttccc gaccaaggag gaactcttcg tggccgcggt cgaacggtcg accggacgca
      241 tggtcgagac tctcgccgcc gtggcttcgg aggccgagaa gaacgggatc gaacccggcg
      301 atgccatggg cctcgcctac ggtgacctgg tcagcagcga ccgcgagttg ctcctgatgc
      361 agcttcacgc ccaggccgtt tcgcccgcca ttccggccgt gcgggaggcg atgcgcaagt
      421 gcttcgccaa cctctacgcg ctcggctccg agcgcaccgg cggcaacggc gaagagatga
      481 agaactggtt cgcccacggc atgctgatca acgtgatgac cgcgatcaat gccgaagagg
      541 tggacgaacc ctgggccgtg gccctgaccg acttcgaagg ggactgcttc ccgcgcgaga
      601 gctgaccggc agccgaccgc acgagcggga ggactgaacc gacgcggcca ggaggccgct
      661 ttttcagacc cgattagtga gtgagcaatc acacattatc aggaaatcaa agtgagtaac
      721 cagtcacaca ccacacagga agtaaccgaa acgggctcgc aggggcccga ccgaccaaac
      781 gaatcaaacg gggcttcggc tccgggaagg aggccgtcat gacggccagc atcacaagca
      841 gaccgggcga gagctccgga aacaggagcg ccatctggac gctcatcatc accgggctgg
      901 cgctcttcat gacctccctc gacaacctgg tcgtcggggt cgcgttgccc tcgatccggg
      961 ctgacctcgg tggcacgatc gaatcgctcg aatggaccgt gaacgcctac accctggcct
     1021 tcgcagttct gctgatcacc ggcgccgcac tcggtgaccg cttcggccgc cgccgcatgt
     1081 tcgcgatcgg ggtgggactc ttcaccgcgg ccagtgccgc ctcggcgctg gcccccacgc
     1141 tcgatgccct ggtcgcggca cgggcactgc agggtgccgg ggcggcgatc gtcgtgccgc
     1201 tcaccctgac cctggtctcg ggagccttcc cggccgaaaa gcggggggcg gcactcggga
     1261 tctggggcgg catcagcgga ctgggcgtcg cgctcggacc gctcttcggc ggtgccgtcg
     1321 tcgaaggcat ctcctggcac tggatcttct ggctgaacgt gccggtcggc ctcgccctga
     1381 tccccctggc cctgcgtcgg ctgcccgagt cgaccggccc cgacaaggcc ctcgacctgc
     1441 ccgggctggg tctggtcggc accgggctct tcggcctgac cttcgggatc atccgcggcg
     1501 agtcgctcgg ctggaccagc accacggtgc tcgcctcgct ggtcaccggg gccctcctga
     1561 tcggtgcctt catcgtctgg gagctgaggg cccgggagcc gatgctgccg atgcggttct
     1621 tccgttcgcg cgggttcagc gccaccaacg gactttcgtt cgcgatgttc ttcggaacct
     1681 tcggggcgat cttcctgctc agccagttct tccaggtcgc gcagggcctc gggcccctcg
     1741 acgccgggat caggaccctc ccctggaccc tgatgccgat gttcgtggcg ccgctggccg
     1801 gcttcctcag cgaccggatc ggcacccggc cgctgatctt caccggcctc gtgcttcagg
     1861 ccggcggtct cgcctggatc acggccatct ccagcccggt caccgccttc ggtgatttcg
     1921 tgaccccctt catgatggcc ggcaccggca tggcgctggt cttccccacc gccgccgcga
     1981 cggtggtcgc ctcggtccgg gaagaggaaa ccggcaaggc ctcgggagcg accaacgcga
     2041 ttcgcgaaat cggcggggtc atgggagttg ccgtgctctc gtcgatcttc gcggccaacg
     2101 gcggcttcac cagcccccag gccttcaccg acggggtcgt ggcggcaatg ccggtcgccg
     2161 tggccgtgct ggcggtcggt
//
LOCUS       DAOQFI010000322         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29253_length_2178_cov_3.470561, whole genome shotgun sequence.
ACCESSION   DAOQFI010000322 DAOQFI010000000
VERSION     DAOQFI010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29253_length_2178_cov_3.470561"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1443
                     /locus_tag="PLE93_06300"
     CDS             226..1443
                     /locus_tag="PLE93_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNH86692.1"
                     /translation="MNQTSDAPKRSLRSVFLAALTGGLVVAVAGMIAIAAGWIGKTET
                     TVQSLTATTTNASSKNDQNLVNQIYNRDSDGVGFITASGISTGSTNPFDPFGQQQGTS
                     TATGSGFLIDTDGHMVTNNHVIEGAKKITVTLGDSDAVYDASVVGTDPSTDLAVIKVD
                     APESEMKPLALGDSTDVKVGDPVVAIGNPFGLDRTVTTGIVSALQREISSLTNYAISN
                     VIQTDAAINPGNSGGPLIDANGNVIGVNSQIATGSGSSTSGNVGIGFAIPSNTVKDVV
                     GQLIEDGKAEHAYLGISGATINEQLANALNLGTDTGVLVQEAPKDGPAAQAGIKAGDT
                     AVTIQGQRVMAGGDVITKVNGTDLGSMEDLIDAVNGAKVGDEMELEVQRDGQTSTVTV
                     KLGTRPDDTQPSK"
     gene            1477..2115
                     /locus_tag="PLE93_06305"
     CDS             1477..2115
                     /locus_tag="PLE93_06305"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNH86693.1"
                     /translation="MKVKVCGITNRDDAEAAVEHGAWAIGLIHHKESPRFVKASVAAG
                     IGEEFRRRCEVVGVFVNPTIERVVQAAENAQLTMIQLSGNEGANFCSEVNRRTGLKVI
                     KAFHISSQADVKASTAYRFTDFHLFDTGRKGVYGGTGETFDWDLLVSYHSPIPTILAG
                     GLNPDNVTEAIRIVRPDAVDVASGVEREPGIKDRGKLASFFQAARYTPVAGA"
BASE COUNT          429 a          738 c          678 g          333 t
ORIGIN      
        1 gaccagcgcg gctgaatgcc gcgcgtgcgt ccgtcccggc tacccccacc cgaccgcacg
       61 gcgtaacctc ctggccccgc ggcgtggccg gacggccccg cggcgtggcc ggacggcccc
      121 gtggcacggt cggaagggcc gggattccca gccggttccc aggtttagga aaccttagaa
      181 tttggcttag gaatggtcaa tgagggtggt ctttatttcc ccgtcatgaa ccagacttcc
      241 gatgccccaa agcgctctct gcgctccgtg ttcctggccg ccctcaccgg cggcctcgtt
      301 gtcgccgtag ccggcatgat cgcgatcgcc gccggctgga tcggcaaaac cgagacgacc
      361 gtgcagtcgc tgaccgcgac caccaccaac gcctcctcga agaatgacca gaacctggtc
      421 aaccagatct acaaccgcga cagcgacggg gtgggcttca tcaccgcctc gggcatctcc
      481 accggttcga ccaatccctt cgatccgttc ggccagcagc agggcaccag caccgcgacc
      541 ggttccggtt tcctgatcga caccgacggt cacatggtca ccaacaacca cgtgatcgag
      601 ggcgcaaaga agatcaccgt cacgctcggt gattcggacg cggtctatga cgcgagcgtg
      661 gtcggcaccg acccctcgac cgacctggcc gtgatcaagg tcgacgcgcc ggaaagcgaa
      721 atgaagccgc tcgcgctcgg tgactcaacc gacgtaaagg tcggtgaccc ggtcgtcgcg
      781 atcggcaacc cgttcggcct cgaccgcacg gtcaccaccg gcatcgtctc cgcccttcag
      841 cgggagatat cgagcctcac caactacgcg atttcgaacg tgatccagac cgacgccgcg
      901 atcaaccccg gcaactccgg cggaccgctg atcgacgcca acggcaacgt gatcggagtc
      961 aactcgcaga tcgccaccgg ttccggctcc agcaccagcg gcaacgtcgg catcggtttc
     1021 gcgattccct cgaacaccgt caaggacgtt gtcggccagc tgatcgagga cggcaaggcc
     1081 gaacacgcct acctcggaat cagcggcgcg acgatcaacg agcagcttgc caacgcgctc
     1141 aacctcggaa ccgacaccgg cgtcctggtt caggaagcgc cgaaggacgg ccccgctgcc
     1201 caggccggaa tcaaggccgg tgacaccgcg gtcacgatcc agggccagcg ggtgatggcc
     1261 ggaggcgacg tgatcaccaa ggtcaacggc accgatctcg gttcgatgga agatctgatc
     1321 gatgccgtca acggggcaaa ggtcggcgac gagatggaac tcgaagtcca acgcgatggt
     1381 cagaccagca cggttacggt caagctcggc acccggcccg atgacaccca gccgtcgaaa
     1441 tgacggccgg acgggcggtt gtcataccct tgactggtga aggtaaaggt ctgtggaata
     1501 accaatcggg atgacgccga agccgccgtt gaacacggcg cctgggcgat cgggctgatc
     1561 catcacaagg agagcccccg gttcgtcaag gcgtcggttg cggccggcat cggcgaggag
     1621 ttccgtcgcc gctgcgaagt ggtcggcgtt ttcgtcaacc cgaccatcga aagggtggtc
     1681 caggcggccg agaacgcgca gctgacgatg atccagctga gcggcaacga gggcgcgaac
     1741 ttctgcagcg aagtgaaccg tcgcaccggc ctgaaagtga tcaaggcttt tcacatctcc
     1801 agccaggcgg atgtcaaggc cagcaccgcc taccgcttca ccgatttcca ccttttcgac
     1861 accggccgca aaggcgttta cggcggcacg ggagagactt tcgactggga cctcctggtc
     1921 agctaccact cgccgatccc gaccattctg gccggggggc tgaaccccga caacgtgacc
     1981 gaggcgatcc ggatcgtccg gcccgacgcg gtggacgttg cttcaggggt cgagcgcgag
     2041 ccggggatca aggaccgcgg caagctggcc agcttctttc aggcagcccg atacaccccg
     2101 gtggcaggcg catgagcggc catttcgcgg agtccacggc cggcgagctg ccgggccgct
     2161 tcggccccta cggcggcc
//
LOCUS       DAOQFI010000323         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29270_length_2177_cov_3.821395, whole genome shotgun sequence.
ACCESSION   DAOQFI010000323 DAOQFI010000000
VERSION     DAOQFI010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29270_length_2177_cov_3.821395"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1116)
                     /locus_tag="PLE93_06310"
     CDS             complement(160..1116)
                     /locus_tag="PLE93_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNH86694.1"
                     /translation="MLLALVAAACASLAYGAVHIPLGEVIDTLLGQKVPEASRQIIEV
                     LRLPRTVEAIVVGAALGVAGACLQGALNNPLASPDVIGVTAGAGFGAMLVLLFSPASV
                     ALLPISALAFGLIAAAMVFLIGFTGRGGGNITRIILAGIAIAALFSAATAGLLSAYPD
                     RVPNAVFFLVGGLTSSDGWDTLETIWPYLAVGFVIAAFMIRPLDRLMLGDDVAASLGS
                     RPRLIRLTAAFAAALLASAAAAIAGLLAFLGLVIPHLVRLLGGTRNHTFVIPVSALAG
                     AALLTAADTVARTIAAPIEMPVGPFLVVLGVPLFLWLLRKAV"
     gene            complement(1197..>2177)
                     /locus_tag="PLE93_06315"
     CDS             complement(1197..>2177)
                     /locus_tag="PLE93_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH86695.1"
                     /translation="GPIPSPPRCVPRLLAYPSKESSPQMTTAAARPQFRFSRIFGALL
                     LLAAVLAGFAFVTPQADAAKQKRVVAISPFAAEAIVQMGARKNLVAVGQTIGASDHHN
                     KLIRGIPKLKLSHPNGPNLEVMAKLKPDVVFSSERWAAGTKALNRLGIRVVYADPEKL
                     GQVKPSVSKIGRVLGKGRQAAALNRRISKSFSKSTRPSTKHPKVLVVLGIGNTAMAFL
                     HNSWGGQLVDKAQANLVTGGAKADGGFARLSDEVVVAQNPDIIIVVPHGNSDDIDQVY
                     DYIMNNDAWQSTTAVEKGKVFVSIHNELLQAGTNVGSVISGLRTKWLKNW"
BASE COUNT          371 a          703 c          770 g          333 t
ORIGIN      
        1 cggcgccgtt gggcccgacg atcgcgagca gctcgccccg ccggagatcc aggctggccg
       61 attcgaggat ctcctcgccg ccgatcgtga cctggacctc tttcgcctcg agcagcggtg
      121 ccttgcgctc gaaggcggcg gttcttctgc cgggggccat cagaccgcct tcctcaagag
      181 ccagaggaag aggggcacgc ccagcaccac gaggaaggga ccgaccggca tctcgatcgg
      241 tgcggcgatg gtccgggcaa cggtgtctgc cgcggtcaaa agagccgctc cggccagggc
      301 cgaaaccggg atcacgaagg tgtggttcct ggtcccgccg agcagacgga ccagatgcgg
      361 gatgaccagg ccgaggaagg cgagcaggcc ggcgatcgcg gcagcggcgg aggcgagcag
      421 ggcggcggcg aaggcggcgg tgaggcggat caggcggggc cgggagccga gcgaggcggc
      481 aacgtcatcg ccgagcatca gccggtccag cggccggatc atgaaggcgg cgatcacgaa
      541 gcccaccgcc aggtagggcc agatcgtctc gagcgtgtcc cagccgtccg aggaggtcag
      601 gccgccgacc agaaagaaga cggcgttcgg cacccggtcc ggataggccg acagaagccc
      661 cgcggtcgcc gccgagaaca gggcggcgat cgcgatgccg gcgaggatga tccgggtgat
      721 gttgccgccg ccgcgcccgg tgaagccgat caggaaaacc atggcggcgg cgatcaggcc
      781 gaaggcaagg gcgctgatcg gcagcagcgc cacgctggcc gggctgaaca ggaggaccag
      841 catcgcgccg aaacccgctc cggcggtgac cccgatcacg tccggcgagg cgagcgggtt
      901 gttgagtgct ccctgcaggc aggccccggc cacgccgagc gccgccccga ccacgatcgc
      961 ctcgaccgtg cggggcaacc gcaacacttc gatgatctgc cggctggcct cggggacctt
     1021 ctggcccagc agggtgtcga tcacctcgcc gagcgggatg tggaccgcgc cgtacgcaag
     1081 cgaagcgcag gccgccgcga ccagggcgag gagcattccg gccgcgacga atatcggtct
     1141 ggctgaagtc agctcactcc cccggtttcg ggatcaggac cggacgggcc ggccgttcac
     1201 cagtttttca gccacttggt ccgcagcccg ctgatcactg aaccgacatt ggtgccggcc
     1261 tggagcagct cgttgtggat cgaaacgaag accttcccct tctcgaccgc ggtcgtggac
     1321 tgccaggcat cgttgttcat tatgtagtcg tagacctgat cgatgtcatc gctgttgccg
     1381 tgaggcacca cgatgatgat gtccgggttc tgggccacga ccacctcgtc ggagagccgg
     1441 gcaaagcctc cgtcagcctt cgccccgccg gtgaccaggt tggcctgggc cttgtcaacc
     1501 agctgcccgc cccacgagtt gtggaggaag gccatcgcgg tgttgccgat gccgagcacg
     1561 accagcacct tcgggtgttt ggtcgagggc cgggttgact tgctgaagct cttgctgatc
     1621 cgccggttca gggcggccgc ctgcctgccc ttgccgagaa cccggccgat cttgctcacg
     1681 gacggcttga cctggccgag cttttccggg tccgcataga ccacccggat gcccagccgg
     1741 ttcagagcct tggtgccggc agcccagcgc tccgaactga agacgacgtc gggcttcagc
     1801 ttcgccatca cctcgaggtt cggcccgttc gggtggctca gcttgagctt cgggatgccg
     1861 cggatcagtt tgttgtggtg atcggaggcg ccgatcgtct ggccgaccgc gaccaggttc
     1921 ttcctcgcgc ccatctgaac gattgcctcg gcggcgaagg ggctgatcgc cacgacccgt
     1981 ttctgtttgg cggcgtcagc ctgcggagtg acgaaggcga atccggcgag gacggcagcg
     2041 agcagcagga gcgccccgaa aatccggctg aatcgaaact gggggcgtgc ggcggcggtc
     2101 gtcatctgcg ggctggactc cttcgagggg taggcaagta accttggaac acagcgcgga
     2161 ggcgaaggta ttggacc
//
LOCUS       DAOQFI010000324         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29273_length_2177_cov_3.420829, whole genome shotgun sequence.
ACCESSION   DAOQFI010000324 DAOQFI010000000
VERSION     DAOQFI010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29273_length_2177_cov_3.420829"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1407)
                     /locus_tag="PLE93_06320"
     CDS             complement(76..1407)
                     /locus_tag="PLE93_06320"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNH86696.1"
                     /translation="MPGLVIVGTQWGDEGKGKVTDLLAKDASAIVRFQGGNNAGHTIV
                     HDGEEYKFHLIPSGILHEDKYCVIGNGVVVDPRVLLEEIEGLHRAGVSASNLRVSSNA
                     HLIMPYHVLVDAAEELKLGKLSIGTTRRGIGPAYADKAYRIGIRVQDLLDEKILRKKI
                     MAALEHKKKSLQELAVRRRKLHKEAEPDEEIQIDDRLDLHTITEHYLSYGHRLEPYIA
                     DTARLCWNELDAGNKVIFEGAQAALLDLDHGTYPFVTSSNPIAGAACVGAGVGPTDIE
                     EVWGIVKAYSTRVGSGPFPTELFDEVGEYMSDKGHEFGTTTGRRRRCGWLDLVALKYA
                     VRLNRLSHLAMTKLDVLTGLDTIRVAVRYRSKEGALLEDFPYHQSILHNATPEYVDLP
                     GFSEDITGCRTEEDLPPAAREYISFISDYVGVPINLVGVGPARDEVVWIQS"
     gene            complement(1426..>2177)
                     /locus_tag="PLE93_06325"
     CDS             complement(1426..>2177)
                     /locus_tag="PLE93_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH86697.1"
                     /translation="GRDLMAAGEFEASGNGQPPCPYGACDGNGWILNEDGSATECRCR
                     QPRLAKARASGVNSVLPKRYRGVSFDRPPVTEIHPGAVTVVRDWIERLDENLDQGNGL
                     WLMGDTGTGKTTLAMLVSKEALRRDHTVAIYSMPSLLTRIRATYGAEAGEESYDQFFE
                     RLCEVDLLHIDDLGAEKQTEWVLEQLYALVNERYEREKSIVVTTNITDQGELEQQIGP
                     RTVSRLTEMCGESVVPLFGEDARQKPQLRDG"
BASE COUNT          328 a          694 c          736 g          419 t
ORIGIN      
        1 tgggaggggc gggttgatcc cggagggcgg gcaaagaccc gcccgactgc ggtcagtccg
       61 tgccggcccg actgcttatg actggatcca gaccacctcg tcgcgggccg gtccgacccc
      121 gaccaggttg atcggcaccc cgacgtagtc gctgatgaag ctgatgtatt cccgggcggc
      181 cggcggcaga tcctcctcgg tccggcagcc ggtgatgtcc tcgctgaatc cgggcaggtc
      241 aacgtactcc ggagtcgcgt tgtggaggat gctctggtgg tagggaaagt cctcgagcag
      301 cgcaccttcc ttcgagcggt agcgcaccgc gaccctgatc gtgtccaggc cggtcaggac
      361 gtcgagcttg gtcatcgcca ggtgtgaaag ccggttcagc cgcaccgcgt acttgagggc
      421 gaccaggtca agccagccgc agcggcgacg ccggccggta gtggtgccga attcatgccc
      481 cttgtccgac atgtactcgc cgacttcgtc gaagagttcg gtcgggaagg gccccgaacc
      541 gacccgggtc gagtaggcct tgacgatgcc ccagacctct tcgatgtcgg tcggtccgac
      601 cccggccccg acgcaggcgg ctccggcgat cggattcgag gaagtgacga agggataggt
      661 gccgtgatcg aggtcgagca gggccgcctg ggcgccctcg aagatcacct tgttgccggc
      721 gtcgagctcg ttccagcaga gccgggcggt gtcggcgatg tagggctcca gccggtggcc
      781 gtagctcagg tagtgctcgg tgatcgtgtg caggtcgagc cggtcatcga tctggatctc
      841 ctcgtccggc tcggcctcct tgtgcaactt gcgccgcctg accgccagct cctggagcga
      901 cttcttcttg tgctcgaggg cggccatgat cttcttgcgg aggatcttct cgtcgagcag
      961 gtcctggacc cggatcccga tccggtaggc cttgtcggcg taggccgggc cgatcccgcg
     1021 gcgggtggtg ccgatcgaga gcttgcccag cttgagttcc tcggccgcat cgaccagcac
     1081 gtggtagggc atgatcaggt gggcgttcga tgaaacccgc aggttcgagg cggagacgcc
     1141 ggcccgatgc agcccctcga tttcctccag cagaacccgg ggatcgacga ccacgccgtt
     1201 gccgatcacg cagtacttgt cctcgtgcag gatgccggaa gggatcaggt gaaacttgta
     1261 ctcctcgccg tcgtgcacga ttgtgtggcc ggcgttgttg ccgccctgga agcgcacgat
     1321 cgcggaggcg tccttggcca gcaggtcggt gaccttgccc ttgccttcat cgccccactg
     1381 ggttccgact attacgagtc ctggcatgaa gagggcagtc tagcttcagc cgtcccggag
     1441 ttgcggcttc tgccgggcgt cctcgccgaa tagcgggacc acgctttcac cgcacatctc
     1501 ggtcagccgc gaaaccgttc gggggccgat ctgctgctcg agttcgccct ggtcggtgat
     1561 gttggtggtg accacgatcg acttctcgcg ctcgtagcgt tcgttgacca gggcgtagag
     1621 ctgttcgagc acccattcgg tctgcttttc ggcgccgagg tcatcgatgt ggagcaggtc
     1681 gacctcgcag agtcgctcga agaactggtc gtaggactct tcgccggcct ccgccccgta
     1741 ggtcgcccga atccgggtaa gcaggctggg catcgagtag atcgcgaccg tgtgatcccg
     1801 cctgagcgct tccttcgaga ccagcatggc gagggtggtc ttgcctgtgc cggtgtcccc
     1861 catcagccag agtccgttgc cctggtcaag gttctcgtcg agccgttcga tccagtcccg
     1921 gacgacggtt accgcaccgg ggtggatctc ggtcaccggc ggacggtcga aggaaacgcc
     1981 gcggtagcgc ttcggcagga ccgagttgac tccggaagcg cgggccttcg ccagccgggg
     2041 ctgccggcac ctgcattcgg ttgccgagcc gtcctcgttc agaatccagc cgttgccgtc
     2101 acaggcgcca taggggcagg gcggctggcc gttgccggat gcctcgaatt cgccggccgc
     2161 catcagatcg cgtccgc
//
LOCUS       DAOQFI010000325         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29358_length_2174_cov_3.470505, whole genome shotgun sequence.
ACCESSION   DAOQFI010000325 DAOQFI010000000
VERSION     DAOQFI010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29358_length_2174_cov_3.470505"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLE93_06330"
     CDS             complement(<1..560)
                     /locus_tag="PLE93_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNH86698.1"
                     /translation="MSENENGTEAGQNLLPGTAPLTALTKQLHERRGKARLGGGLEQV
                     EKQHAAGKLTARERIDLLVDEGSFNEIGIHGRPHYSQPAMQSKEAPADGVVTGWGDVD
                     GRPVAIVAYDFTVMAGSMGQTGEIKVTRIREMALQKRMPLVWLLDSAGARIQEAAGSL
                     FAGSGHLFREEVQMSGVVPMVAAMMGP"
     gene            complement(560..1357)
                     /locus_tag="PLE93_06335"
     CDS             complement(560..1357)
                     /locus_tag="PLE93_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH86699.1"
                     /translation="MSQATPDFGKIRYEVADHVATITLDDPDTRNALSDQLLGELISA
                     LHLAREDTEVRVVVLASSHETTFSAGGNLAAFGGDANAVQKFEGAELFVESFKLLGQL
                     GKPSIVAARGHVLAGSLGLALACDLIVASEEATFGTPEINVGLFPFMIMALIYRNVPR
                     KKANEMLLLGGRMEAKDAKEAGIVNFVAKKKDFDLVLEDWTTMLARKSPLIMKLGKDA
                     MWRQMDMDLAAALDYLRSQLAIELSSEDAIEGVKAFFEKREPEWKGR"
     gene            1433..>2174
                     /locus_tag="PLE93_06340"
     CDS             1433..>2174
                     /locus_tag="PLE93_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH86700.1"
                     /translation="MSTETVIKPDHEAGRKHFIFTQEHEDLRESMTDWVKKELHPHRM
                     EWEETKWPDSAMKRAGELGYLGLCFPEEYGGQGGDYYYSLVRAEALSYSGSGGLGMGF
                     AVQTDMVLPPIHLLGTEEQKQKYLAPGITGEKIGALGITEPGAGSDVAGIRTTAILDG
                     DEYVINGSKTFITNAARADFMVLVTKTDPDAGHDGITLMLIDIRDEDGNDLPGFSVAQ
                     NLEKMGMHASDTGELAFEDFRVPADSVLG"
BASE COUNT          377 a          729 c          680 g          388 t
ORIGIN      
        1 ggacccatca tcgcggcaac catcggcacc acgccggaca tctggacctc ctcgcggaag
       61 aggtggccgg aaccggcgaa gagcgatccc gctgcttcct ggatccgggc accggccgag
      121 tcgagcagcc agaccagagg catccgcttc tgcagtgcca tctcgcgaat cctggtgacc
      181 ttgatctcgc cggtctggcc catcgagccg gccatcaccg tgaagtcata ggcaacgatc
      241 gcgaccggcc ggccatcgac gtcaccccag ccggtgacca ctccgtcggc tggcgcctcc
      301 ttggactgca ttgccggctg cgagtagtgc gggcgtccgt ggatgccgat ctcgttgaag
      361 ctgccctcgt cgaccagcag gtcgattcgc tcgcgggcgg tcagcttgcc tgccgcatgc
      421 tgcttctcga cctgctcaag gccgccgccg agccgggcct tgccccgccg ctcgtggagc
      481 tgcttggtca gcgcggtcag cggcgccgtg ccgggcagca ggttctgccc ggcctcggtc
      541 ccgttttcgt tctcgctcat cagcgtccct tccattccgg ctcgcgcttt tcgaagaagg
      601 ccttgacgcc ttcgattgcg tcttcgctgg agagctcgat tgcgagttgc gagcggaggt
      661 agtcgagcgc ggcggcgagg tccatgtcca tctgccgcca catcgcgtcc ttccccagct
      721 tcatgatcag cggcgacttg cgggccagca tcgtggtcca gtcctcgagg accaggtcga
      781 agtccttctt cttggcgacg aagttgacga tgcccgcctc ctttgcgtcc ttggcctcca
      841 tccttccgcc gagcagcagc atctcgttcg ccttcttgcg cggcacgttg cggtagatca
      901 gcgccatgat catgaagggg aacaggccga cgttgatctc gggagtgccg aaggtcgctt
      961 cctcgctggc cacgatcagg tcgcaggcaa gggcaagacc cagcgagccg gccagcacat
     1021 ggccgcgggc ggcgacgatc gacggcttcc cgagctgacc gagaagcttg aaactctcga
     1081 cgaagagctc ggccccttcg aacttctgga cggcgttggc gtcgcctccg aaggcggcca
     1141 ggttgccgcc ggccgagaag gtggtctcat gcgaggaggc cagcacgacc acccgaacct
     1201 cggtgtcttc gcgggccagg tggagggccg agatcagctc ccccagcagc tggtcggaaa
     1261 gggcatttcg cgtatcggga tcgtcgagcg tgatcgtcgc gacatggtcg gcgacctcgt
     1321 acctgatctt cccgaagtca ggagtggctt ggctcatgcc gctgacgata ccagctagcg
     1381 gagagtagga agtgctactg tccacttcgt aaacttcacc gaggagatcc gcgtgtccac
     1441 cgaaaccgtc atcaagcccg accatgaagc cggccgcaag cacttcatct tcacccagga
     1501 gcacgaagac ctgcgcgagt cgatgaccga ctgggtcaag aaggaactcc acccgcaccg
     1561 catggagtgg gaagagacca agtggcccga ttcggccatg aagcgggccg gcgagctcgg
     1621 ttacctcggc ctctgctttc ccgaggagta cggcggacag ggcggggatt actactactc
     1681 gctggttcgg gccgaggcac tttcgtactc gggctccggt ggcctcggca tgggcttcgc
     1741 ggtccagacc gacatggtcc tcccgcccat tcacctgctc ggcaccgaag agcagaagca
     1801 gaagtatctg gctccgggaa tcaccggaga gaagatcggc gcactcggca tcaccgagcc
     1861 cggcgccggc tccgacgtgg ccgggatccg caccaccgcg atcctcgacg gcgacgagta
     1921 cgtgatcaac ggttccaaga ccttcatcac caacgccgcc cgggccgact tcatggtgct
     1981 ggtcacgaag accgaccccg acgccggtca tgacggcatc accctgatgc tgatcgacat
     2041 ccgcgacgag gacggcaacg acctgcccgg tttctcggtc gcccagaacc tggaaaagat
     2101 gggcatgcat gcttccgaca ccggcgagct cgccttcgag gatttccgcg tgccggcgga
     2161 ttccgtgctc ggtc
//
LOCUS       DAOQFI010000326         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29401_length_2172_cov_3.401512, whole genome shotgun sequence.
ACCESSION   DAOQFI010000326 DAOQFI010000000
VERSION     DAOQFI010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29401_length_2172_cov_3.401512"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLE93_06345"
     CDS             <1..316
                     /locus_tag="PLE93_06345"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNH86701.1"
                     /translation="IGKGVLLLVRGGKTVRVIHVSAGGPGNETPTGRYRIFRKELQSW
                     SVPFSTWLPYASYFNNGIAFHEYPDVPPYPASHGCVRVPAPEAPLVYRFARLGTVVVV
                     RS"
     gene            803..1684
                     /locus_tag="PLE93_06350"
     CDS             803..1684
                     /locus_tag="PLE93_06350"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNH86702.1"
                     /translation="MSKRKIDVRIRNWRLLPVGTGAGVYAVGRPIESRRAICMAATLV
                     AGDNSMVSGQAAADLWGFREFRGQVDVVRVNSRKPRTFWLDGEGVIGRHKVVVHRSRH
                     LPREDRTRQHGIPVMNVARLFVDLSASLGDKPLYESFKAADRKGYLNKTELARCAALG
                     RGWKGIRRYRKLVYRRHPDMKDSASPKEGGFLDICERYDLGRPVVNRRMGRHFPDFLF
                     ENEGLLVEVEGAEFHSGRLAYLDDTHRENELRFKVRQVIRFSSEEIDLEPEKVASLVK
                     QEKAKCLLLKSLEASAA"
     gene            complement(1681..1977)
                     /locus_tag="PLE93_06355"
     CDS             complement(1681..1977)
                     /locus_tag="PLE93_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HNH86703.1"
                     /translation="MEASDPEQDLRDQAVQSLNDKHDFMIHLATFIGVSILLILIWAF
                     TGADGFFWPIFPIAGWGVFGVIPHYWSVYRSKGITEDKIQAEMEKIKSQQGSDR"
BASE COUNT          397 a          621 c          722 g          432 t
ORIGIN      
        1 gatcggcaag ggcgtgctgc ttcttgtcag gggtgggaag acggtccggg tgatccacgt
       61 ttcggcgggc gggcccggta acgaaacccc gaccggtcgc tacaggattt tccgcaagga
      121 actccagtcc tggtcggttc cgtttagcac ctggcttccg tatgcctcgt acttcaacaa
      181 cgggatcgcc ttccacgaat accccgacgt gccgccgtac cccgcgtccc acggttgcgt
      241 ccgcgtcccc gcgccggaag ccccgctggt ctaccggttc gcccggcttg gaaccgtggt
      301 cgtggttcgc tcctgaggcg tagcggacat cgcgttgatc atcggagctt caagaaccca
      361 cccgggattc ccgggggcga tcggccacgg attcggtggt caatcgtccc cggaatgcgg
      421 tcagcgtcct gccctcgaag gttccggggg cgttctgcga ccgatcgggt ggcagatcgc
      481 ccccggttgg gttgggaggt cgggtggccg actcgcggga ctttgggcct gaggtcggat
      541 ctgaggtcgg gtggccgaca gggccggcga agggctttaa ggggacgaac ttgttgcact
      601 tcggtcagtg gtccgtcgct gaattgttgc tccggttgca cacatttgag gtttctccgg
      661 ccatgctttc ccgatggacc tccgcagcgg acaatcttcg gtcgagagcg acagatcgtc
      721 gcggccaggc tcgctggaag tacttcaagt cgcgaaccgg caatacggaa tggttacgag
      781 ggcgcagctg cgggatcttg ggatgagcaa gcgcaagatc gatgtccgga ttcgaaactg
      841 gaggctgctg ccggtcggca ccggcgccgg ggtatatgcg gtggggcggc ccatcgaaag
      901 ccggagggcc atctgcatgg cggccaccct ggttgccggc gacaactcga tggtctcggg
      961 gcaggccgca gcagatctct ggggctttcg ggagttcaga ggtcaggttg acgtggttcg
     1021 cgtcaacagc cgcaagcccc gcaccttctg gcttgacgga gagggtgtca tcggccggca
     1081 caaggtggtg gtccaccgga gccggcacct gccgcgcgag gatcgaaccc gccagcatgg
     1141 tattccggtg atgaacgtag ccaggctgtt tgtggacctc tcggcgtcac tcggtgacaa
     1201 gcctctctac gaatcattca aggcagcgga caggaagggg tacctgaaca agacggaact
     1261 cgccaggtgc gctgcgctgg ggaggggatg gaagggcatt cggcggtacc ggaagctcgt
     1321 ttaccggcgg catcccgaca tgaaagattc agcgtcaccc aaggaaggcg gattcctgga
     1381 catctgcgag cgctacgacc tgggcagacc agtcgtgaac aggcgaatgg gcaggcactt
     1441 tcccgacttc cttttcgaga acgaaggtct gctggtagag gtagagggtg ccgaattcca
     1501 ctccggtcga ctcgcttacc tcgacgacac tcaccgtgag aatgagctca ggttcaaagt
     1561 ccgtcaggtg atccgcttct caagcgaaga gatcgatctc gagcccgaaa aggtggcgag
     1621 tttggtcaaa caggaaaagg cgaaatgcct cctcttgaag agccttgagg cttcggccgc
     1681 ctagcggtcc gaaccttgct ggctcttgat cttttccatc tccgcctgga tcttgtcttc
     1741 ggtgattcct ttcgatctgt aaacggacca gtaatgcggg atcacgccga aaactcccca
     1801 gcccgcgatc ggaaaaatcg gccagaagaa gccatctgcg ccggtaaatg cccagatcag
     1861 gatcaggagg atgctcaccc cgatgaaggt cgcgaggtga atcatgaagt cgtgcttgtc
     1921 gttgaggctc tggacggcct ggtctcgaag atcctgttcc ggatcgctgg cttccatcat
     1981 tcctcctggt agctggggtt tgagggaatc gtagccgtct ttcctccggc cggttccggc
     2041 tgagcgacag gcagtccctc ccttccgggg gcgaaaggcc accggattcg tggtcgttcg
     2101 cccccggaaa ggggggcgaa ggaggggttg gttgcgggcc ggggtttccg ggggagatca
     2161 acacccgaat cg
//
LOCUS       DAOQFI010000327         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29486_length_2169_cov_3.327342, whole genome shotgun sequence.
ACCESSION   DAOQFI010000327 DAOQFI010000000
VERSION     DAOQFI010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29486_length_2169_cov_3.327342"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..844
                     /locus_tag="PLE93_06360"
     CDS             188..844
                     /locus_tag="PLE93_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86704.1"
                     /translation="MRLPIAVGFAVAVFVLGAAGLTLNSHRPPADHEPVVSVPSGSRN
                     VVVSLRLDDCGEPIEGEVRFSSPRSRIGSPVRFYSDAGGWQRLGLTGARARVRRASFS
                     EEEATRKRSLLSCYLQLPILKGAADGYEVHLRLSDQMEVEKDSSVPSPGAYASGAWVW
                     KCGPDVTCPAFAAVSYSIEDGTKQVIILVLASVFGALIALLVGEVLIEWARKRFREPD
                     "
     gene            complement(850..>2169)
                     /locus_tag="PLE93_06365"
     CDS             complement(850..>2169)
                     /locus_tag="PLE93_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNH86705.1"
                     /translation="QQLKYLADHDPLTGLYNRRRFEEELSSAIDSGHGRGIAILVIDL
                     DNFKFVNDSFGHTIGDRLIVRTAELLKSRLRSDDTLARQGGDEYVVVLRDISPEDALT
                     TAEELVRLIARDVRIVGAEHSARVTASIGVAVSSEISPVPEETLMMQADIAMYEAKDA
                     GRNGARVFQPGEDSHVTLGIDWAGKLRSALENEELLVYAQPIHRLNGEGPPHHELFVR
                     MRDRSGSIIMPGAFLPTAERHDLVQELDSWVISKAISTLAELEGDGCPRLQINLSGRT
                     VGDPKLPEFVQGELSRAGVDPSNLIFEVTETSAIGNMTGAQEFSARMNELGCGFALDD
                     FGTGFASFYYLKHLAFDYVKIDGEFVQNLASDPVNRLLVKALVEISKGMGKRTVAEQV
                     EDARTLEVLREFGVDFVQGHYLGRPRPLDRSELASLPALPLDTALPG"
BASE COUNT          351 a          717 c          707 g          394 t
ORIGIN      
        1 cgcctggcgc aagtcccgga cctgagcccg ttcagttgac ggtccaggac ctcgtcgagt
       61 tctcgctctt cgtcggactg tcgttctgct gatcctcctg ctcgcgagcc tggtccgccg
      121 ccgccccggc gggttgcggg gaaccaccct gaaagagaag ctgaaacgct tcggtcgatt
      181 cgctctggtg cgcctgccga tcgcggttgg attcgccgtg gcggttttcg tgctgggggc
      241 ggccggcctc acgctgaaca gccaccggcc gccggccgac cacgagccgg tcgtctcggt
      301 tccttcgggg tcgcggaacg tggtggtttc cctccggctg gatgactgcg gtgagccgat
      361 cgagggcgaa gtccgcttca gctcgccccg aagcaggatc gggtccccgg tcaggttcta
      421 ctccgatgcc ggtggctggc agagactcgg cctcaccggc gctcgggccc gggtccggcg
      481 cgcaagcttc agcgaagagg aagcgacccg gaaacgaagc ctgctctcct gctacctgca
      541 gctgccgatc ctcaaggggg cggccgacgg gtacgaagtg cacctcaggc tctccgacca
      601 gatggaggtc gagaaagact catcggtccc ctcgcccggg gcctatgcct ccggcgcctg
      661 ggtctggaaa tgcggcccgg atgtaacctg ccccgctttc gccgcagtca gctactcgat
      721 cgaggacggc accaagcagg tgatcatcct ggtcctcgcc tccgtcttcg gcgcgttgat
      781 cgcactgctg gtcggcgagg tactgatcga gtgggcgaga aaacgcttcc gcgaaccgga
      841 ctgagcggtt cagcccggga gcgccgtatc caacggcagg gcgggcagcg aagccagttc
      901 ggagcggtcc agcggccggg gccggccgag atagtggccc tggacgaaat cgaccccgaa
      961 ctcgcggagc acctccaggg tcctcgcgtc ttcgacctgc tcggcgaccg tccgcttgcc
     1021 catgcctttt gaaatctcca ccagggcctt gaccagcagc cggttgaccg ggtccgaggc
     1081 caggttctgg acgaactcgc cgtcgatctt cacgtagtcg aacgccagat gcttgaggta
     1141 atagaaggac gcgaatccgg tcccgaaatc atccagggcg aaaccgcagc cgagctcgtt
     1201 catacgggcc gagaactcct gggccccggt catgttgccg atcgcgctgg tctcggtcac
     1261 ttcgaagatc aggttggacg ggtccacccc cgccctcgag agctcgccct gcacgaattc
     1321 gggcagcttc gggtccccga ccgtgcgtcc ggacaggttg atctggagcc gcgggcaacc
     1381 gtccccttcg agctcggcca gggtcgaaat cgccttgctg atcacccatg aatcgagttc
     1441 ctggaccagg tcatggcgtt cggcggtcgg caggaaggcg ccgggcatga tgatcgaacc
     1501 gctgcgatcg cgcatccgga cgaaaagctc gtgatgcggc gggccctctc cgttgagccg
     1561 gtgaatcggc tgcgcgtaga ccagcagctc ttcgttttca agcgcgctgc gcagcttccc
     1621 ggcccagtcg atgcccagcg tgacatgtga atcctcgccg ggctgaaaga ccctggcacc
     1681 gttgcggccg gcgtccttgg cttcgtacat ggcgatgtcg gcctgcatca tcagcgtttc
     1741 ctcgggcacc ggcgagatct ccgacgaaac cgcgacgccg atgctggccg taacccgggc
     1801 agaatgttcg gccccgacga tccgcacgtc ccgggcgatc agcctgacca gttcctcggc
     1861 ggtggtcaaa gcgtcttcgg ggctgatgtc gcgaagcacc accacgtatt cgtcgccacc
     1921 ctgccgggcg agggtgtcat cggagcgcag ccggctcttc aacagctcgg ccgtccgcac
     1981 gatcaaccgg tcgccgatcg tgtggccgaa actgtcgttg acgaacttga agttgtcgag
     2041 gtcgatgacc aggatcgcga tcccccggcc gtgtccggag tcgatcgccg agctgagttc
     2101 ctcttcgaag cgacgccggt tgtagaggcc ggtcagcggg tcgtgatcgg ccagatactt
     2161 gagctgctg
//
LOCUS       DAOQFI010000328         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29609_length_2164_cov_3.285917, whole genome shotgun sequence.
ACCESSION   DAOQFI010000328 DAOQFI010000000
VERSION     DAOQFI010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29609_length_2164_cov_3.285917"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="PLE93_06370"
     CDS             complement(<1..1560)
                     /locus_tag="PLE93_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH86706.1"
                     /translation="MSNPDEREDRNIPDPVELKLYRGRIAEAASRAALEKEPDPAGIW
                     AEAAGPGSYTWLWTTGIKRAPTVLGWAALVICLLATLGGFVDIPLFTGELLAAPQMPV
                     FSSVTLGMVAFALLAMRPGAAESAVQAGVALCYFTIAVCMALLALRMAGIDPDVWGSD
                     ALRSPSVAALVNNPALALCLLTIDRDPPRYRWQRALIPLVGIIFLISVLTKGYQAALD
                     ANVQTDISILGSATVILIYVGYVFLRPDRGLAGFLSSTGPGPSMARLLVPTAVVIPLT
                     MVMVDLISEGLESGESAFIKGLDRLLMLVLLIWMITYASIRLQRFYDRWKQANSELAS
                     QASVLTRMSEGVVVLRIADSRIVLTNPQFDEMHGFDRGELIGRNIDVIAPDDLSEDEV
                     QMRVDVLHELADKGETSYENRSVRKDGTDIWSRVNGVITTVPEHGPVLILVKYDVTGE
                     RIAKSAGRDAEMRFRQVFEQSPIGLCLVTPEGRFEKVNRSFESITGYSAGELSEMTFA
                     DITHPDDLEEDV"
     gene            complement(1646..>2164)
                     /locus_tag="PLE93_06375"
     CDS             complement(1646..>2164)
                     /locus_tag="PLE93_06375"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH86707.1"
                     /translation="TNHLDIESREALEDALLGFPGAVVLISHDRALLEAVGSRTLVCE
                     NGELTNHMTGWAEYQKRRDEAAEAEQEKAAASRPAGKSGNARRGGSPGKEVARTRRRV
                     ENLEKKIEQAEARLAEIEDELSDPAAWSTPEKSEKATRRHDRAKEKVASLYAEWEEAD
                     QVARETAEAATA"
BASE COUNT          369 a          717 c          701 g          377 t
ORIGIN      
        1 gacgtcctct tcgaggtcat ccggatgggt gatgtcggca aaggtcattt ccgagagctc
       61 acccgcggag tagccggtga tcgactcgaa gctccggttc accttctcga aacggccctc
      121 gggagtgacc aggcaaagac cgatcgggct ctgctcgaaa acctgccgga agcgcatctc
      181 ggcgtcgcgc ccggccgatt tcgcgattcg ttccccggtc acgtcgtact tgacgaggat
      241 cagaaccggc ccgtgttccg gcacggtcgt gatcacgccg ttgacccggc tccagatgtc
      301 ggtgccatcc ttcctgaccg agcggttctc gtagctggtc tcccccttgt cggccagttc
      361 gtgaagcacg tcgacgcgca tctggacttc atcctcggag agatcgtccg gggcgatcac
      421 atcgatgttc cggccgatca actcgccccg gtcgaacccg tgcatctcat cgaactgcgg
      481 gttggtcagc acgattcgcg aatcggcgat ccgcaggacc acgacgccct cggacatcct
      541 tgtcaggacg ctggcctgcg aggcaagttc cgaattggcc tgtttccagc ggtcgtagaa
      601 gcgctgcagg cgaatcgagg cgtaggtgat catccagatc aggaggacca gcatcagcag
      661 gcggtcgagc cccttgatga acgcggactc accggactcg agcccttccg agatcaggtc
      721 gaccatgacc atggtcagcg ggatcaccac cgccgtcgga acgagcagcc gggccattga
      781 cggtccggga ccggtcgatg agagaaaacc ggccagcccc cggtcaggcc ggaggaacac
      841 gtacccgacg tagatcagga ttacggtcgc cgatccgagg atgctgatgt cggtctgaac
      901 gttggcatcg agcgcggcct ggtaaccctt ggtcaggacc gagatcagga agatgatgcc
      961 gaccagcggg atcagggccc gctgccagcg gtagcggggc ggatcgcgat cgatcgtcag
     1021 caggcagagt gccagggccg ggttgttgac gagcgcggcg accgacggcg agcggagcgc
     1081 gtccgaaccc cacacgtccg gatcgatacc agccatccgc agggccagca gcgccatgca
     1141 gacggcgatc gtgaagtagc agagcgccac gccggcctgg accgccgatt cggcagcgcc
     1201 ggggcgcatc gccagcaggg cgaaggcgac cattcccagg gtcaccgacg agaacaccgg
     1261 catctgcggg gcggcgagaa gctcgcccgt gaacaacggg atgtcgacga aaccgcccag
     1321 ggtcgccagc aggcaaatca ccagggcggc ccagccgagc acggtcgggg cccgcttgat
     1381 cccggtggtc cagagccagg tgtagctgcc ggggccggcc gcttcggccc agatcccggc
     1441 ggggtcgggc tccttttcga gcgccgcccg gctcgcagcc tcggctatcc gcccccggta
     1501 cagcttcagc tcgaccggat cgggaatgtt ccgatcttcc cgctcatctg gattcgacaa
     1561 gtgcttctcc aaaggaactc ctgactgcga aactaacacc tgctttcccg ggaacgggaa
     1621 cgccgaaacg catggtggcc gcggttcagg cggtggcggc ttcggccgtc tccctggcga
     1681 cctgatcggc ctcttcccac tcggcgtaga gcgaggctac cttctccttc gcccggtcat
     1741 gccgccgggt tgccttctcc gacttctcgg gagtcgacca ggccgcggga tcggagagtt
     1801 cgtcctcgat ttcagccagc cgggcctcgg cctgttcgat cttcttttcg aggttctcga
     1861 cccggcggcg ggttcgggcc acctccttgc cgggcgagcc gccgcgccgg gcgttgcccg
     1921 acttcccggc cggccgggaa gcggcagctt tttcctgttc ggcctccgcg gcttcatccc
     1981 ggcgcttctg atactccgcc cagccggtca tgtggtttgt cagttcgccg ttctcgcaga
     2041 ccagggtccg gctgccgacc gcttccagca gggcgcgatc gtgagaaatg aggacgaccg
     2101 ccccggggaa accgagcagg gcatcctcga gtgcttcccg gctttcgatg tccagatggt
     2161 tggt
//
LOCUS       DAOQFI010000329         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29671_length_2162_cov_3.737067, whole genome shotgun sequence.
ACCESSION   DAOQFI010000329 DAOQFI010000000
VERSION     DAOQFI010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29671_length_2162_cov_3.737067"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLE93_06380"
     CDS             complement(<1..715)
                     /locus_tag="PLE93_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86708.1"
                     /translation="MTDSGNVTPTSEFDRRAEVSALLATDETWLGDIYRSYSEGKTAI
                     QIAEEFSVTPSPVYSYFRTIEALLDGTSTAPPTVASGWAGRIRGWLKNKPLSPELREA
                     LTRQEQLLSEASSDPAATKAESEEAVTKSKAAEAENVPGVYVYTLPHYVRYPFDPKTG
                     RTLLKVGHSSTSAHFRASAQARSTELPEDPWLLRIYPAESSAEAEKKFHAFLKAADHN
                     GVKGNRTGAEWFLTSLKFLD"
     gene            complement(731..1219)
                     /locus_tag="PLE93_06385"
     CDS             complement(731..1219)
                     /locus_tag="PLE93_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNH86709.1"
                     /translation="MREVQIYGVSFDMVGKQPIVLLKTIDDNRYLPIWIGHPEAAAIL
                     MKLQGADTPRPMTHDLIVDMLDQVETKVERISVNELRDNTFFATITLSVSGTEIEIDS
                     RPSDALALAVRLQAPIFVAEEVIEESSIEFDQGIEDNEQVVEKFKDFLDDVSPEDFLQ
                     DS"
     gene            complement(1378..1451)
                     /locus_tag="PLE93_06390"
     tRNA            complement(1378..1451)
                     /locus_tag="PLE93_06390"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1415..1417),aa:Ile,seq:gat)
     gene            complement(1539..>2162)
                     /locus_tag="PLE93_06395"
     CDS             complement(1539..>2162)
                     /locus_tag="PLE93_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNH86710.1"
                     /translation="IIAGERRWRAAQMAGVEKVPVVIRDSDEADRLEVALIENMVREN
                     LNPVEEAKACAALVEDLGLSKEELGKRVGRSRPQISNLIRLLDLPDDVLEMLEGGELS
                     EGHGRAILQVPDHADRRKLAKQARKEGWSVRQTEAGARALAAAPKKGGRKQGLSAEES
                     EAVAEATERLETKLDRPVKIRPKGKGLVVEIELDDLAQAAEIARRLK"
BASE COUNT          370 a          667 c          673 g          452 t
ORIGIN      
        1 gatcgaggaa cttgagcgat gtcaggaacc actcggcgcc agtgcggttg cccttcacgc
       61 cattgtgatc agccgccttg aggaaggcgt gaaacttctt ttcggcttca gccgagctct
      121 cggccggata gatccgaagt agccagggat cctccggaag ctcggttgag cgcgcctgcg
      181 cgctggcacg aaagtgcgct gacgtagacg aatgaccaac cttgaggaga gtcctgcccg
      241 tcttgggatc aaacggatac cggacgtagt gaggaagcgt gtagacatag acacccggca
      301 cgttttcggc ctctgccgct ttggactttg tgaccgcctc ttcagattcg gccttggtcg
      361 ctgccgggtc gcttgaagcc tcggaaagga gctgctcctg tctcgtcagc gcctctcgca
      421 gctctggact taatggcttg ttcttgagcc agccgcggat acgcccggcc caccccgatg
      481 ccaccgtggg cggagcggtg gacgttccat caagaagcgc ttcgatcgtc cggaaatacg
      541 agtagacggg actaggtgtg acgctgaatt cctcggcgat ctggatcgcg gttttcccct
      601 ccgagtaact ccgatagatg tccccgagcc aggtctcgtc cgttgcgaga agcgcggaaa
      661 cctccgccct tcggtcgaac tcactggttg gtgtcacatt gccggaatcg gtcacgcgac
      721 ggatgggcct ctaggagtcc tggaggaagt cttcggggga gacgtcgtcg aggaagtcct
      781 tgaacttctc gaccacctgc tcattgtctt cgatgccctg gtcgaactcg atcgaggatt
      841 cctcgatcac ttcctcggcc acaaagatcg gtgcctggag cctgaccgcc agggccagcg
      901 cgtcggaggg gcgtgaatcg atttcaatct cggtgcctga gaccgagagc gtgatcgtgg
      961 cgaagaaggt gttgtcgcga agctcgttga ccgagatccg ttccaccttg gtttcaacct
     1021 ggtcgagcat gtccacgatc aggtcgtggg tcatcggccg cggcgtgtcg gcaccctgga
     1081 gcttcatcag gatcgcggcc gcttcagggt gcccgatcca gatcggcagg tagcgattgt
     1141 cgtcgatcgt cttcagcaaa acgatcggct gcttgccgac catgtcgaag ctgacgccat
     1201 agatctgaac ctcacgcatg gatcccgatt ataggtagga cgagccctgg tcacctcatc
     1261 agccgttccg ccgtgtcggc tcgaacggag gtcctcccat ccgtcatttt gtgtgagcgg
     1321 gaaggccggt tgagattgga gggcgggcgc ggacccgccg gactgcggtc aagcctatgg
     1381 gcgatcctgg attcgaacca gggacctcat ccttatcaga gatgcgctct aaccaactga
     1441 gctaatcgcc ctcgaggaac cgaggattct aatgggggtc gggcccgggt gtcgtgaggt
     1501 gaggagacac cgcgccggga cgagcccgga gcctcgactt acttgagacg cctggcgatc
     1561 tcagccgcct gggcgaggtc gtcgagttcg atttcgacca ccagcccctt ccccttcggt
     1621 ctgattttca ccgggcgatc gagtttggtt tccagccgct ccgttgcttc ggcgacggct
     1681 tcactctcct cggccgacag accctgcttg cggccgccct tcttcggcgc cgcagcgaga
     1741 gcgcgagccc cggcctcggt ctgacgaacc gaccagcctt ccttcctggc ctgtttggcc
     1801 agcttgcggc gatcggcgtg gtcgggaacc tggaggatcg cgcgtccatg gccttcggaa
     1861 agttcgcccc cttcgagcat ctcgagcacg tcgtcgggca ggtcgagcag ccggatcagg
     1921 ttggagatct gggggcggct gcggccaacc cgcttgccga gttcctcctt gctgagaccg
     1981 agatcctcga ccagggcggc gcaggccttt gcctcttcca ccggattcag gttttcccgg
     2041 accatgttct cgatcagtgc gacctcgagc cggtcggctt catcggaatc acggatgacc
     2101 accgggacct tttcgacccc ggccatctgt gcggcccgcc agcgccgctc gccggcgatg
     2161 at
//
LOCUS       DAOQFI010000330         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29752_length_2159_cov_3.085551, whole genome shotgun sequence.
ACCESSION   DAOQFI010000330 DAOQFI010000000
VERSION     DAOQFI010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29752_length_2159_cov_3.085551"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLE93_06400"
     CDS             complement(<1..738)
                     /locus_tag="PLE93_06400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH86711.1"
                     /translation="MSVLAILAGAAFATLAMIGPAAAAGKSLDYVALGDSYSAASGVL
                     PLDPEAPVQCLRSTLNYPHVLAAKLGAELTDVTCGAAETRHFGESQYPGVAPQLDALS
                     KDTDLVTMTIGGNDSSVFIGSILSCGTLGVASLGQGSPCKDTFGDLDLARAARAHHLK
                     TDDRLTVEESRRARLLNVVADLGQRTEPNPAVVGERDLDLAKFLGRKYRPERTHGLLA
                     IADVGQAPRRFLLHLPQLTGDLRHRRPH"
     gene            complement(876..1283)
                     /locus_tag="PLE93_06405"
     CDS             complement(876..1283)
                     /locus_tag="PLE93_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNH86712.1"
                     /translation="MAELEGRSLEIVKEGANYAVVAVPTDDGSVQSVIVWAHADDDGN
                     VILNSAEGRAWPQNLRKAGRATVTMMADGNPYEYVSIRGRLIGDTQEGADDDINMLAK
                     KYIDADEYPFRQPGEVRVTFTLEPEKVNYLKQG"
     gene            complement(1320..>2159)
                     /locus_tag="PLE93_06410"
     CDS             complement(1320..>2159)
                     /locus_tag="PLE93_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH86713.1"
                     /translation="GKSTLARSLLQLLRPSSGSVRFRGVELTEISNRKLRPIRRDMQM
                     IFQDPYTSLNPRKRVGQIIGEPLRIHGLAEGREIERRVREKLDQVGLSGSYYDRYPHE
                     FSGGQRQRIGIARALAPEPSLIVADEPVSALDVSIQGQIVNLMADLQRELELTYLFVA
                     HDLGVVRQVSDRIAVMYLGKIVEIGPAELVYSKPIHPYTASLLAAVPIPDPRTNAQRE
                     RRPLEGDVPSPSDPPSGCRFHTRCPYATEVCEQQEPALIDYGQGHFAACHHPLGRPDP
                     AGE"
BASE COUNT          355 a          682 c          746 g          376 t
ORIGIN      
        1 atgcgggcga cggtggcgga ggtctcccgt cagttgcggc agatgcagca ggaagcgtcg
       61 gggggcctgg ccaacgtcgg caatcgcgag caggccgtgc gtacgctcgg gacggtactt
      121 tcggccgagg aacttggcga ggtcgagatc acgctcgccg accacagccg ggttcggctc
      181 ggtgcgttgg ccgaggtcag cgacgacatt caggagccgc gctcggcggc tctcctcaac
      241 ggtcagccgg tcgtcggttt tgaggtggtg cgcgcgcgcg gcgcgagcga ggtcgaggtc
      301 gccgaaggtg tccttgcagg ggctgccctg acccagcgag gcaaccccga gcgtgccaca
      361 gctcaggatc gagccgatga agacgctgct gtcattgccg ccgatggtca tcgtgacgag
      421 gtcggtgtcc tttgagagcg cgtcgagctg tggcgccacc ccggggtact gggactcgcc
      481 gaagtgcctg gtttcggccg cgccacaggt cacgtcggtc agctcggcgc ccagctttgc
      541 agccagaaca tgcggatagt taagagtcga gcgcaggcac tggaccgggg cttcggggtc
      601 gagcggcaga accccggaag cggcgctgta ggagtcgccg agtgccacat agtcgagtga
      661 cttgccggcc gcggccgccg gcccgatcat ggcgagcgtg gcgaatgccg caccggccag
      721 aattgcgagg acggacacac aacgactgcg aagcttcatc aaatctcccg gggtagtcga
      781 gaacaccagc gttcctgagc gctctgtgac taccccggga ttcgaactcc aaaccgggaa
      841 gatgcgggcc aggccaaccc tcccgggttc tgcggctaac cctgcttgag gtagttgacc
      901 ttctcgggtt cgagggtgaa agtgacccgg acctcgcccg gctggcggaa cggatactcg
      961 tcggcgtcga tgtacttctt ggccagcatg ttgatgtcgt cgtcggcgcc ttcctgggta
     1021 tcaccgatca gccggccgcg gatcgacacg tactcgtagg ggttgccgtc ggccatcatc
     1081 gtcacggtcg cccggcccgc cttgcgcagg ttctgcggcc aggcccgacc ctcggcactg
     1141 ttcaggatca cgttgccgtc gtcgtcggcg tgcgcccaga cgatcaccga ctgcaccgag
     1201 ccgtcatcgg tcggcacggc gacgaccgca tagttcgcac cttccttgac gatttcgagc
     1261 gaacgacctt cgagttctgc cacttcgacc tcctgggttg tttgaagaca ggaccgaatc
     1321 tactccccgg ccgggtccgg tcgaccgagg ggatggtggc aggcggcgaa atgaccctgg
     1381 ccgtagtcga tcagggccgg ctcctgttgc tcgcagactt cggttgcgta ggggcagcgg
     1441 gtgtggaaac ggcaacccga gggcgggtcc gaggggctcg gaacgtcacc ctcgagcggc
     1501 cggcgctccc tctgtgcgtt ggttctcggg tcgggaatcg ggacggccgc cagcagcgag
     1561 gccgtgtagg gatggatcgg tttcgagtag acgagttcgg ccgggccgat ctcgacgatc
     1621 ttgcccagat acatcacggc gatccggtcg gacacctgcc ggacgacgcc caggtcatgc
     1681 gccacgaaga ggtaggtcag ctccagctcg cgctggaggt cggccatcag attcacgatc
     1741 tgcccctgaa tcgaaacgtc gagcgccgag accggttcgt cggcgacgat caggctcggt
     1801 tcgggcgcaa gggcacgggc gatgccgatg cgctggcgct ggccaccgga gaactcgtgc
     1861 gggtaacggt cgtagtagct gccggagagc ccgacctggt ccagcttctc ccgcacccgg
     1921 cgctcgatct cacggccctc ggccagcccg tggatacgaa gcggctcgcc gatgatctgc
     1981 ccgacccgct tgcgcggatt cagcgaggtg taggggtcct ggaagatcat ctgcatgtcc
     2041 cgccggatcg gccggagttt ccggttgctg atttccgtca gctcgacccc gcggaacctg
     2101 actgaaccgg agcttggcct gagcaactgc agcagcgacc tcgccagggt cgacttccc
//
LOCUS       DAOQFI010000331         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29801_length_2157_cov_2.998097, whole genome shotgun sequence.
ACCESSION   DAOQFI010000331 DAOQFI010000000
VERSION     DAOQFI010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29801_length_2157_cov_2.998097"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PLE93_06415"
     CDS             complement(<1..882)
                     /locus_tag="PLE93_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNH86714.1"
                     /translation="MNGAGSFRLAVAQPEIDREAPEASRVTEAAAMIREAGRGQADLI
                     LFPESFPGPITAGLEYDAAPDLGAACRDSGVAACWGRVEPIGEGRFVTVVRLTDRTGG
                     EAGRYVRSHPATGDVHPVLSGVPMAPGPEPGIFELDGLRLGIVVCSELWNPELCRVLA
                     LRGAEVLLAPAGGGFHSVAENWQLMARARAIENEMFVALTQQLFDGESGSALIAGPEG
                     VLSAGTGPGLVIADLDLERARWLRSQDDSMQEPKPFSSLPGLLRARRPELYGELGAPQ
                     EGLYDFELHKTLLRFVQL"
     gene            complement(879..1466)
                     /locus_tag="PLE93_06420"
     CDS             complement(879..1466)
                     /locus_tag="PLE93_06420"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HNH86715.1"
                     /translation="MAMAEITTEVPHGTARRFDLGTGDLIRIETLDGAQGGDFSFPGF
                     DQALTRNKLGWKRFGRPWLVYDAQAGDSLFDVDGEATFELIALEGEGVADIMYPGCWD
                     EVYEDRRPGCQDLISAALTIPRRDLAGMLSFFIGYSADNSAYRGLAGIDLKPGDHLTF
                     RALRDVSCAVSACPDRDIPGWRVGALFVSVTEAGK"
     gene            complement(1550..>2157)
                     /locus_tag="PLE93_06425"
     CDS             complement(1550..>2157)
                     /locus_tag="PLE93_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNH86716.1"
                     /translation="PADPAPRAGEGFQGRIFRIDRDLRERMVGSSWHRGCPVRLADLR
                     LLRLSYRDFDGDVRRGYLVVHETAAPRMVKAFGRLFNAGYRIRRMRLVDAYGADDHRS
                     MNADNTSAFNCREVAGQPGVWSRHAYGRAIDINPVENPYVSGSHVSPARGRPFADRYP
                     RRKGMISAGGRVVRIFAAIGWGWGGDWAGAKDYQHFSASGD"
BASE COUNT          357 a          753 c          727 g          320 t
ORIGIN      
        1 cagctgcacg aagcgcagca gcgtcttgtg cagctcgaag tcgtagagcc cctcctgtgg
       61 cgccccgagc tcaccataga gctcggggcg tcgggctctc agcagccccg gcagggagct
      121 gaacggcttc ggttcctgca tcgagtcgtc ctggcttcgc agccagcggg cccgctcgag
      181 atcgaggtcc gcaatcacga ggcccggtcc cgttccggcg ctcagcacac cctccggccc
      241 ggcgatcagg gccgaaccgg actcgccgtc aaagagctgc tgggtgaggg cgacgaacat
      301 ctcgttctcg atcgcccggg ccctcgccat cagctgccag ttctcggcca cggaatggaa
      361 gccgccgccg gccggggcga gcagtacctc cgccccgcgc agggccagga cgcggcagag
      421 ctccggattc cagagttctg aacagaccac gatcccgagc ctgagtccgt ccagctcgaa
      481 gatgcccggc tccgggcccg gcgccatcgg caccccggaa aggaccggat ggacgtcacc
      541 ggttgcggga tggctgcgaa cgtagcggcc ggcctcgcct ccggtccggt cggtgagccg
      601 caccacggtg acaaaacggc cctcgccgat cggctcgacc cggccccagc aggccgcgac
      661 gccggagtca cggcaggcag ccccgagatc gggggccgcg tcgtactcga ggccggcggt
      721 gatcggtccg gggaagctct ccgggaagag gatcaggtca gcctggccac gaccggcttc
      781 ccggatcatt gccgcggcct cggtcacccg ggaggcctcg ggagcttcgc ggtcaatctc
      841 cggctgggca accgccaggc ggaagctgcc cgccccgttc acttgccggc ctcggtgacc
      901 gatacgaaca gggcgccgac cctccagccg gggatgtccc ggtcggggca ggccgaaacc
      961 gcgcagctga cgtcgcgcag cgcccggaag gtcaggtggt cgccgggctt caggtcgatg
     1021 ccggccagac cccgataagc ggagttgtcg gccgagtagc cgatgaagaa ggagagcatg
     1081 ccggccaggt cccgccgggg aatcgtgagc gccgccgaga tcaggtcctg gcaacccggt
     1141 cgccggtcct cgtagacctc gtcccagcaa cccggataca tgatgtcggc gactccctcc
     1201 ccttcgaggg cgatcagttc gaaggtcgcc tcgccgtcga cgtcgaagag cgaatcaccg
     1261 gcctgggcgt cgtagaccag ccagggccgg ccaaaccgct tccagccgag cttgttccgg
     1321 gtcagggcct gatcgaaacc ggggaagctg aagtcgcctc cctgcgcgcc gtccagggtc
     1381 tcgatccgga tcagatcccc ggtccccaaa tcgaacctcc gggcggtccc gtgaggcact
     1441 tcggttgtga tctccgccat tgccatcgca cccgctttca gtcgggtcgc actcctttat
     1501 cgaagcggtg cttacgaact cagtgcgacg gtgtagaggg attttagcct tagtcgccgg
     1561 aggcggagaa gtgctggtaa tccttggccc cggcccagtc gccgccccat ccccagccga
     1621 tcgcggcgaa gatccggacg accctgccgc cggcgctgat cattcccttg cggcgggggt
     1681 agcggtcagc gaaaggccgg cccctggccg gagaaacgtg gctccctgac acatacgggt
     1741 tctcgaccgg attgatgtcg atcgcccggc cgtaggcatg ccgggaccag accccgggct
     1801 ggccggcgac ctcgcggcag ttgaaagccg aggtgttgtc ggcgttcatc gagcggtggt
     1861 catcggcgcc gtaggcatcg accaggcgca tgcgacggat ccggtagccg gcgttgaaga
     1921 gacggccaaa agccttgacc atgcggggcg cggccgtctc gtggacgacc aggtatccac
     1981 gccggacgtc gccgtcgaaa tcccggtagc tcagcctgag cagccgcagg tcggcaagcc
     2041 ggaccgggca tccccgatgc caggaggaac cgaccatgcg ttctctcaga tcccggtcga
     2101 tcctgaagat cctgccctgg aacccttccc ccgcccgggg cgccggatcg gcggggg
//
LOCUS       DAOQFI010000332         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29881_length_2154_cov_2.624107, whole genome shotgun sequence.
ACCESSION   DAOQFI010000332 DAOQFI010000000
VERSION     DAOQFI010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29881_length_2154_cov_2.624107"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLE93_06430"
     CDS             <1..374
                     /locus_tag="PLE93_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86717.1"
                     /translation="LELTGDALGSQAHTARRAARGLQEVLGEMRDCDLAVPPARQAIT
                     DLEAEDVKTIIDRARGSRDLDPILVQAAPNRAAYRGLELAVVHMTARRQMLYERFKRL
                     WHEQSRQGVWVALETSLKVES"
     gene            377..>2154
                     /gene="ppk1"
                     /locus_tag="PLE93_06435"
     CDS             377..>2154
                     /gene="ppk1"
                     /locus_tag="PLE93_06435"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNH86718.1"
                     /translation="METTPDPSWFFNRELSWLDFNQRVLELAEDDSAPLLERVKFCAI
                     FASNLDEFFMVRVAGLWDQVDAGIDARGPDGLSPREQIQAIRSRSLELDRRLTRVFER
                     KLKPQLTENGIKVATLADLNGTERAELDRIFDQQVFPALTPLVIGLGRPFPYISNLSL
                     SIAVLLRDRETSTRVLARIKVPKELLGRFIELSSEKERVLVPLEEVIAANLDQLFPGV
                     EVLDHSYFRVTRDADFTVSDEADDLLEAVQEEVRRRRFGEVVRLEVADGMNPELKELL
                     VELLRLQQNEVFEAGGLLDLTDLWDVVKLGGFQELRDKPWAPVTQPRLQGEDGDPPDI
                     FAAIRQRDVLVHHPYDSFSSSVERFIAQAVSDPDVLAIKQTVYRTSEDSPLVSALIKA
                     SERGKQVVCMLELKARFDEEANIEWAKRLEEVGVHVVYGIPGLKTHIKALLVVRREGD
                     QVRNYVHIGTGNYHSTTARLYTDLGLFTADPEIGADVAEMFNLLTGYSRPADYRRVLV
                     SPHSMRSSILAEIEATVAAHKAGREAKILMKMNSLVDRTCIEALYRASQAGVEVEINV
                     RGICCLRPGVPELSENIRVVSLVGRFL"
BASE COUNT          373 a          709 c          702 g          370 t
ORIGIN      
        1 cgctggagct gaccggtgac gccctcggat cccaggctca caccgcccgc cgcgcggccc
       61 gggggctgca ggaggtgctc ggcgagatgc gtgactgtga cctggcggtg ccgccggccc
      121 ggcaggcgat caccgacctt gaggccgaag acgtgaagac gatcatcgat cgcgcccgcg
      181 gcagccggga cctcgatccg atcctggtcc aggccgcacc gaaccgggcg gcctaccgcg
      241 gcctcgaact cgccgtcgtt cacatgacgg cccggcggca gatgctctat gaacgcttca
      301 aacgactctg gcacgagcag tcccgccagg gcgtctgggt cgctcttgaa acctcactga
      361 aagtagaatc ctgaccttgg aaacgactcc cgatccctcc tggttcttca atcgcgaact
      421 ctcctggctt gatttcaacc agcgggttct tgagctggcc gaggacgatt cggcgccgct
      481 cctggaacgg gtcaagttct gcgcgatctt tgcctcgaac ctcgacgaat tcttcatggt
      541 ccgggtggcc gggctgtggg atcaggtcga tgccggaatc gacgcccggg gcccggacgg
      601 cctctcgccg cgggaacaga tccaggcgat ccgcagccgg tcgctggaac tcgaccgccg
      661 gctgaccagg gtctttgaac gcaagttgaa gccgcagctg accgagaacg gcatcaaggt
      721 cgcgaccctg gccgacctga acgggaccga gcgggcggag ctggaccgga ttttcgacca
      781 gcaggtcttc ccggcgctga ccccgctggt gatcggtctc ggccgtccct tcccttacat
      841 ctcgaacctc tccctgagca tcgccgtcct gcttcgggac cgcgagacct cgacccgcgt
      901 gctcgcccgg atcaaggtgc cgaaggagct gctcggccgc ttcatcgaac tgagcagcga
      961 gaaggagcgg gtgctggtgc cgctggaaga ggtgatcgcg gccaatctcg atcagctgtt
     1021 ccccggtgtt gaagtgctcg atcactccta tttccgggtt acccgggacg ctgatttcac
     1081 ggtgtccgac gaagccgacg acctgctcga agcggtccag gaggaagtcc gtcgccggcg
     1141 tttcggggag gtcgtgcggc tggaagtcgc cgacgggatg aacccggagc tcaaggagct
     1201 gctggtcgag ctgcttcgtc ttcagcaaaa cgaagtattc gaagccggcg gcctgctcga
     1261 cctgaccgac ctctgggacg tggtcaagct gggcggcttc caggagctgc gcgacaagcc
     1321 ctgggctccg gtcacccagc cccggctgca gggcgaggac ggagacccgc ccgacatctt
     1381 cgcggcgatc cgccagcgcg acgtgctggt ccatcacccc tacgattcct tctcgagttc
     1441 ggtcgagcgg ttcatcgccc aggcggtgag cgatccggac gtgctggcaa tcaagcagac
     1501 cgtttaccgg accagcgagg actcgccgct ggtctcggcc ctgatcaagg cctcggaaag
     1561 gggcaaacag gtcgtctgca tgctggaact gaaggcgcgc ttcgacgagg aagcgaacat
     1621 cgagtgggcc aaacgccttg aagaagtcgg ggttcacgtg gtctacggca tccccgggct
     1681 gaagactcac atcaaggccc tgctggtggt gcggcgggaa ggcgaccagg tccgcaacta
     1741 cgtccacatc ggcaccggca actaccactc gaccaccgcc cggctctaca cggacctcgg
     1801 cctgttcacc gccgacccgg agatcggcgc cgacgtggcc gagatgttca acctgctgac
     1861 cggttactcg cgcccggccg actaccgccg ggtgctggtc tcgccgcatt cgatgcgttc
     1921 ctcgatcctg gccgagatcg aagccacggt cgccgcccac aaggcaggcc gggaggcgaa
     1981 gatcctgatg aagatgaact cgttggtcga ccggacctgc atcgaggcgc tttaccgggc
     2041 ctcgcaggcc ggtgtggaag tcgagatcaa cgtgcgcggg atctgctgcc tcaggcccgg
     2101 ggtgccggag ctttcggaga acatccgggt ggtctccctg gtcggccggt tcct
//
LOCUS       DAOQFI010000333         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29901_length_2153_cov_3.022402, whole genome shotgun sequence.
ACCESSION   DAOQFI010000333 DAOQFI010000000
VERSION     DAOQFI010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29901_length_2153_cov_3.022402"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..893
                     /gene="heR"
                     /locus_tag="PLE93_06440"
     CDS             150..893
                     /gene="heR"
                     /locus_tag="PLE93_06440"
                     /inference="COORDINATES: protein motif:HMM:NF037099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HNH86719.1"
                     /translation="MPKLRRLNVFAAIFFAAQVAVLLVVAEPVTLPVTGQFLTGPPGG
                     GAYGSTELFRLRIDLLVALFLGLAAFDHFVVATWLRGFYEKNIGRGRNPARWYEYSVS
                     ASVMIVLIAMLSGVTDVVALLAIFGVNASMILFGLAMERANAGRTEVDWSPFIYGCVA
                     GAVPWIAIVIQFILSGSNSDGVPGFVYGIFVTLFLLFNCFALNMWLTYRARGRWRDPL
                     FAEKVYIWLSITAKTALAWQVYSGALAGS"
     gene            complement(931..1899)
                     /locus_tag="PLE93_06445"
     CDS             complement(931..1899)
                     /locus_tag="PLE93_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86720.1"
                     /translation="MYESWYARLVSPDGSLALWIRYTIDKLPNQEATGTIWFTLFDGE
                     AERPLARRLVGQPASAPVNTWARIGESTIGPAGLHGECFEAAWQLRFEPRAPVLFHLP
                     RPRFYELPLPKTKPISPVPFGDFSGSVNFGERQISVEGWHGMIGHNWGSEHAERWIWL
                     HGSDFDEDPEAWLDIVMGRIRVAGRLLPWVANGAISTGGEIRRIGGMFASGVNVKETP
                     LRLEAGLPVAGKSYLELEVDSPPNLTVGWQYGDPADPEAEAHEVANCSAAQMKATLTM
                     PGHNAVTNLGTLRGAVYELGMTETDHGIPISDGIPPGHGPGSAVSK"
BASE COUNT          339 a          717 c          683 g          414 t
ORIGIN      
        1 ccgtggattc acgtccgccg attgctccgg ggcggccgca tgaactggaa gctttcctcc
       61 agcccgtcgg cctggggccg gggcaagacc ctggctccgc cctccttcgg cgaccgctga
      121 ccgggggcgc ccgtttgtag ggttcggcca tgccgaagct caggagactc aatgtctttg
      181 ccgcaatctt tttcgcggcg caggtcgcgg ttcttctggt agtcgcagaa ccggtcaccc
      241 tgcccgtgac cggccagttt ttgaccgggc ctcccggagg cggcgcttac gggagcaccg
      301 aactgttcag gctccggatc gatctgctgg tcgcgctctt tctcggcctc gccgccttcg
      361 accatttcgt ggtcgccacc tggctgcgcg gcttctacga gaagaacatc ggcaggggga
      421 gaaacccggc ccgctggtac gaatactcgg taagcgcctc ggtgatgatc gtcctgatcg
      481 cgatgctctc cggcgttacc gatgtcgtag ccctgctggc gatctttggc gtcaacgcct
      541 cgatgatcct tttcggcctg gccatggaaa gggcgaatgc gggacggacc gaagttgatt
      601 ggtcaccatt catctatggc tgcgtggcag gcgccgtgcc ctggatcgcg atcgtgatcc
      661 agttcatcct ctccgggtcc aattcggacg gggtgccggg tttcgtctac gggatcttcg
      721 tcaccctgtt tcttctgttc aactgcttcg cgctcaacat gtggttgacc taccgtgccc
      781 ggggcaggtg gcgtgacccc ctgttcgccg agaaggtcta catctggctg agcatcaccg
      841 ccaagacggc gctcgcctgg caggtctaca gcggagctct ggccggttcc tgagaccagc
      901 ccggccagaa cttttcgcgg agtccttccc tcattttgag accgctgatc cgggaccgtg
      961 gccgggtgga atgccatccg aaatcgggat gccgtgatcg gtttcggtca tgcccagttc
     1021 gtagaccgcg ccacgcaggg tgccaaggtt ggtcaccgcg ttgtggcccg gcatggtgag
     1081 cgtcgccttc atctgggccg ccgagcagtt cgccacttca tgagcttcgg cctccgggtc
     1141 ggcagggtca ccgtattgcc agccgaccgt gaggttcggc ggcgagtcga cctccagctc
     1201 caggtagctc ttgccggcga ccggcaggcc ggcctccaac cggagcgggg tctccttcac
     1261 attgacgccg gaggcgaaca tcccgccgat ccgccggatc tcgccgccag tgctgatcgc
     1321 cccgttggcc acccagggca gcagccggcc ggcgacccgg atccggccca tgacgatgtc
     1381 gagccatgcc tccgggtcct cgtcaaagtc gctgccatgc agccagatcc accgctccgc
     1441 atgttcgctg ccccagttgt ggccgatcat gccgtgccag ccttcgacgc tgatctgacg
     1501 ctcgccgaag ttcaccgaac cggagaagtc cccgaacggc accggggaga tcggtttggt
     1561 cttcggcaag ggaagttcgt agaagcgggg ccgcggcagg tgaaagagaa ccggtgccct
     1621 gggctcgaaa cgaagctgcc aggcggcttc aaaacattcg ccgtggagcc cggccggacc
     1681 gatcgtcgat tccccgatcc gggcccaggt gttgaccggg gccgaggccg gctggccgac
     1741 gaggcggcgg gccagcggtc gctcggcctc gccgtcaaag agggtgaacc agatcgtccc
     1801 ggttgcttcc tggttcggga gcttgtcaat cgtgtacctg atccagagcg ccagcgaacc
     1861 gtccggcgaa accagccggg cgtaccagga ctcgtacatc ccggcgcctg gcccggcctc
     1921 tgtgaatcgc gcgtcagtcg gtgagatcct ccggcccacc cgaatagaac tatcagagcg
     1981 gcgccgaagg aattggcttc tcagcgaaaa gaaagcttgc gaactgcccg gtaggtgttc
     2041 ccgagccggt cccaggcctt gatccggacc tgcgcccgac ccttgccggc cgcaactcga
     2101 cggttcagct tcacgccgac cctgaccgac ctgcccggct tgacccggaa tcg
//
LOCUS       DAOQFI010000334         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29980_length_2150_cov_3.224344, whole genome shotgun sequence.
ACCESSION   DAOQFI010000334 DAOQFI010000000
VERSION     DAOQFI010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29980_length_2150_cov_3.224344"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLE93_06450"
     CDS             <1..203
                     /locus_tag="PLE93_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86721.1"
                     /translation="EPVSKNKDGSRIGSDEYVTLALCIIVPIIGLLFALYYRQQGEKW
                     AGRAMVVGVVALVVWLCLVFLI"
     gene            278..778
                     /locus_tag="PLE93_06455"
     CDS             278..778
                     /locus_tag="PLE93_06455"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNH86722.1"
                     /translation="MAFPELSLRSTSGGEVSLPSAFGERAVLFTYPRTGTPGEPLLPG
                     WDDIPGARGCTPQSCAYRENLAGFRSAGLAVFGLSAQTAEAHAEFHARESIPYELISD
                     PGLKLAETLRLPTFAISSLTLYRRLTMIIEAGQIAKVFYPVFPPDQDAATVLAWLAGD
                     DLAETS"
     gene            811..1734
                     /locus_tag="PLE93_06460"
     CDS             811..1734
                     /locus_tag="PLE93_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86723.1"
                     /translation="MSTPLRLLLYGLSVLAVFAVSFVAGKALIPEDTVSDWTRQADAS
                     SAGHESMAGDHDEAHVGGLSVEQDGYTISPVSSPSVAGQPGELSFRILDPEGKPLTGY
                     SSNHEKQLHLITVRLDGDRFRHVHPRLDLASGTWSIPWSWPAGGSYRVFADFVPQGSS
                     PDQALTLSRSVEVGGEFMPEPKPLSRTDSTGGFKATLRGELEAGSSSELTAHLTRGGR
                     PVTDLEPYLGAFGHLVALREGDLGYLHVHAEDGTEGNPVQGHEHAHSPETAGPDIEFA
                     AEAPTPGRYLLYLDFKVDGRVHTAEFVLEAK"
     gene            1731..>2150
                     /locus_tag="PLE93_06465"
     CDS             1731..>2150
                     /locus_tag="PLE93_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNH86724.1"
                     /translation="MSATRTETGESPDPITRRELDFEISGMTCASCAARIERNLNRLD
                     GIDASVNYATEKARVKVPEGLAPEEIIAEVEKTGYSAALPPDSNQDGTGRDHAGHSGG
                     HDHGGIAGPETDREIEVLRQRLIGSILLAVPVIAMSMF"
BASE COUNT          379 a          717 c          686 g          368 t
ORIGIN      
        1 aagaaccggt aagcaagaac aaggacggca gcaggatcgg ctccgatgaa tacgtcacgc
       61 tggcgctctg catcatcgtg ccgatcatcg ggttgctctt cgccctctac taccgccagc
      121 agggcgagaa atgggccggc cgagcgatgg tcgtcggcgt cgtcgccctg gtcgtctggc
      181 tctgcctggt cttcctgatt tgagccgcga cttcagagag ctcccgcccg acctgccggt
      241 tcccgtcgat gacggggccg cggaccacct cgaggggatg gcgttcccgg agctcagcct
      301 gaggtccacg agcggcgggg aagtgagcct gccctcggcc ttcggcgagc gggccgttct
      361 cttcacctac ccgcggaccg gcaccccggg ggagccgctt ctgcccggct gggatgacat
      421 tcccggcgcc aggggttgta cgcctcagtc ctgtgcctac cgtgaaaacc tggccggttt
      481 ccggtcggcc gggctggccg tcttcgggtt gagtgcccag accgccgaag cccatgccga
      541 gttccacgcg cgggaatcga ttccctacga gctgatcagt gatcccggac tgaaactggc
      601 cgagacgctg aggctgccga cctttgccat ttccagcctc accctgtacc ggcggctgac
      661 catgatcatc gaagcgggac aaattgcgaa ggtcttttat cccgtcttcc cgccggatca
      721 ggacgctgcc acggtgctcg cctggctggc cggggacgac ctggcggaga cctcttgaca
      781 tataccccat aggggtatag ggtgttcgac atgagcaccc cgcttcgact tcttctctac
      841 gggctttcgg tcctggccgt attcgcagtt tccttcgtcg ccggcaaggc cctgattccg
      901 gaagacacgg tttccgactg gacccggcag gccgatgcca gctcggccgg gcatgaatcc
      961 atggcggggg accacgacga agcccacgtc ggcgggctct cggtcgagca ggacggctac
     1021 acgatttccc cggtcagttc cccctctgtg gccggccagc cgggtgagct ctccttccgg
     1081 atcctggacc cggaagggaa acccctgacc ggatactcga gcaatcacga gaagcagctt
     1141 cacctgatta cggttcgcct tgacggtgac cggttcaggc acgtgcaccc cagactcgac
     1201 ctggcctccg gcacgtggtc gatcccctgg tcatggccgg ccggagggtc ctaccgggtc
     1261 ttcgccgact tcgtgccgca gggatcctcc ccggatcagg ctctgaccct gagccgcagc
     1321 gtcgaagtgg gcggcgaatt catgcccgag ccgaagccgc tcagccggac cgactcgacc
     1381 gggggcttca aggcaaccct tcgcggcgag ctcgaagccg gctcgagcag cgagttgacc
     1441 gcacacctga cccgcggcgg ccggccggta accgatctgg agccgtacct cggcgccttc
     1501 ggtcacctgg ttgccctgcg cgaaggtgac ctcggttacc tgcatgttca cgcggaagat
     1561 gggaccgagg gcaatccggt tcagggccac gaacatgccc acagcccgga aaccgccgga
     1621 cccgacatcg agttcgcggc cgaagcgccg actcccggcc gttacctgct ctacctcgat
     1681 ttcaaggtgg atggccgggt acacacggcg gaattcgtac tggaggcaaa atgagtgcca
     1741 cgcggacaga gaccggcgaa agccccgacc cgatcacccg gcgggaacta gatttcgaga
     1801 tcagcgggat gacctgcgcc tcctgcgccg cgcgcatcga gaggaacctg aacaggctgg
     1861 acggaatcga cgccagcgtc aactacgcga ccgagaaggc ccgggtgaaa gtgcccgagg
     1921 gcctggcgcc cgaagaaatc atcgccgagg tggagaagac cggctactcg gccgcactgc
     1981 cgccggattc caatcaggac gggaccgggc gtgaccatgc cggtcattcc ggcggtcacg
     2041 atcacggcgg tatcgcggga ccggaaaccg atcgcgaaat cgaggtcctc cgccaaaggc
     2101 tgattggctc gatcctgctg gcagtcccgg tgatcgcgat gtcgatgttc
//
LOCUS       DAOQFI010000335         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_29982_length_2150_cov_3.135084, whole genome shotgun sequence.
ACCESSION   DAOQFI010000335 DAOQFI010000000
VERSION     DAOQFI010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29982_length_2150_cov_3.135084"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1672)
                     /locus_tag="PLE93_06470"
     CDS             complement(98..1672)
                     /locus_tag="PLE93_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNH86725.1"
                     /translation="MSQSPEERPDLTELRRRQELTLDEARREAVARRHQRGGRTAREN
                     LEDLIDPGSFVEYGRYAIAAQRARFDTDDLISRTPGDGLIAGTATVNGELFGENSRCA
                     VLSYDYTVLAGTQGAVGHLKKDRLFELIEKMKLPTIFFAEGGGGRPGDTDFPVVSALQ
                     VRAFALWAGLSGLVPRVAIVKGRCFAGNAVIAGCADLIVATEDSSLGMGGPAMIAGGG
                     LGEVAPDEVGPVLFQEPNGVLDLVVADEAEATAAAKKLIGYFQGRSPWTDGGESDQAP
                     LREMVPERERRAFDVRPVIETIADPDSVTLLREKFAPELVTALARIEGRPVGVIANDS
                     RHMAGTITSDSADKAARLLQLCESFGLPVISLVDTPGFMVGPAHEQTALVRHTSRLLV
                     AGAALTVPLISVILRRAYGLGAQAMVGGSLHEPLLTVAWPSAHLGPMGLEGAVRLAMR
                     KDLEAIADEEEREKRVRELTALAEQNARAMNAAQLFEIDDVIDPAETRSVIASTLAAA
                     PERVSERAPHRFVDTW"
     gene            1822..>2150
                     /locus_tag="PLE93_06475"
     CDS             1822..>2150
                     /locus_tag="PLE93_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86726.1"
                     /translation="MGLAAVVAAFFVLAGTAYAGAVIQTDPVEYTFLPGPYVQGLGET
                     ATLDNSVSENWHDVVATKNGPDGKPLFSAPLARGGETKVVQGTQYLAAGTYHFICSFH
                     GAGMSGDL"
BASE COUNT          341 a          718 c          744 g          347 t
ORIGIN      
        1 cttgactgcg taaaaagaca acttcaagtt accccataac cgcagaggga cctccgggtt
       61 gcgggtctgg tcgaaagaga ccggcccgcc gccggcttca ccaggtgtca acgaagcggt
      121 gcggcgcacg ctctgagacc cgttcgggcg cggcggccag agtcgaggcg atgaccgatc
      181 tggtctcggc cggatcgatc acgtcatcga tctcgaagag ctgggccgcg ttcatggccc
      241 gcgcgttttg ctcggccagg gcggtcagct cgcgaacccg cttttcccgc tcctcctcgt
      301 cggcgatcgc ctcgaggtcc ttgcgcatcg ccaggcgtac ggcaccctcg agacccatcg
      361 gcccgaggtg ggcgctcggc caggcgacgg tgagcagggg ttcgtggagg ctgccgccga
      421 ccatcgcctg cgcgcccagc ccgtaggccc ggcgcaggat caccgagatc agcggaaccg
      481 tcagggcggc gcctgcaacc agcaggcggg aggtgtgccg gaccagggcg gtctgctcgt
      541 gggccggccc gaccatgaag ccgggagtgt caaccaggga gatgacgggc aggccgaagc
      601 tctcgcaaag ctgaagcaag cgggccgcct tgtcggccga gtcgctggtg atcgtgccgg
      661 ccatgtggcg ggagtcgttg gcgatcacgc cgaccggccg gccctcgatc cgggccagcg
      721 cggtgaccag ttccggagcg aacttctcgc gcagcagggt gaccgaatcc ggatcggcaa
      781 tggtctcgat caccgggcgc acgtcgaaag cccgtcgctc gcgttctggg accatctcgc
      841 ggagcggagc ctggtcgctt tccccgccgt cggtccaggg gctgcggccc tggaagtaac
      901 cgatcagctt cttcgccgcc gcggtcgcct ccgcttcatc ggcgacgacg aggtcaagca
      961 caccgttggg ctcctggaac aaaaccggac cgacctcatc cggggcgacc tcgcccagac
     1021 ccccgccggc gatcatcgcc gggccgccca tgccgagcga ggagtcttcg gtggcgacga
     1081 tcaggtcggc gcagccggcg atcaccgcat tgccggcgaa gcagcggccc ttgacgatcg
     1141 ccaccctcgg gaccaggccc gagagcccgg cccaaagtgc gaaggccctc acctgcaggg
     1201 ccgagacgac cgggaaatcg gtgtcgccgg ggcggccgcc accaccctcg gcgaagaaga
     1261 tcgtcggcag cttcatcttc tcgatcagct cgaagagccg gtccttcttg aggtggccga
     1321 ccgctccctg ggtgccggcc agcaccgtgt agtcgtagga gagtacggcg catcgagagt
     1381 tctcgccgaa gagttcgccg ttgaccgtgg cggtgccggc gatcagtccg tcgccgggcg
     1441 tgcgactgat caggtcatcg gtgtcgaagc gggcccgctg ggcggcgatc gcgtaccggc
     1501 cgtactcgac gaagctgccg gggtcgatca ggtcctcgag gttctcgcgg gcggtgcgac
     1561 cgccgcgctg gtgacgccgg gctacggctt cccggcgggc ctcgtcaagg gtgagctctt
     1621 gccggcgcct gagctcggtc agatcgggcc gttcttcggg actctgcgac attcccgaaa
     1681 actgtcacat cccggcgggc gacgcgccga agccgcaggg ccgagcgcca gattccgggc
     1741 cgtcgcggca tttaccgttg caagcggagt tgatatgcgt cataatggag cgctgtggga
     1801 gggatcagtt ccagaattgc gttggggctg gcggccgtcg ttgccgcctt tttcgtgctg
     1861 gccggaaccg catatgcggg tgccgtgatc cagaccgatc cggtcgagta cacgttcctg
     1921 ccgggtccct acgttcaggg tttgggcgag accgcgactc tggacaactc ggtgtcggag
     1981 aactggcacg acgtcgtcgc gacgaagaac ggccccgatg gcaagccgct cttctcggct
     2041 cccctggccc ggggaggcga gaccaaggtg gtccagggca cccagtacct cgcagccggt
     2101 acctaccact tcatctgctc cttccacggg gccggcatga gcggcgatct
//
LOCUS       DAOQFI010000336         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30033_length_2148_cov_2.374582, whole genome shotgun sequence.
ACCESSION   DAOQFI010000336 DAOQFI010000000
VERSION     DAOQFI010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30033_length_2148_cov_2.374582"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLE93_06480"
     CDS             complement(<1..702)
                     /locus_tag="PLE93_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH86727.1"
                     /translation="MEASGVETGRQAEMNGAGPSGGGGPRVCVIGAGSSGITAMRSLQ
                     EKGIDFVCFEKGSWIGGNWKYDNDNGQSAAYKSLHINTSRQMMEYKAFPMPEDFPDYP
                     DHFQIASYFEDFVDHFGLRSSIRFQTEVESVVPAEGGGWEVTWQNREGESGTDHFSDV
                     MVCNGHHWDKRWPEPAFPGSDTFEGEQMHAHDYRTPEIFEGKRVLVLGIGNSATDIAV
                     EASRHADATFLSMRRG"
     gene            782..>2148
                     /locus_tag="PLE93_06485"
     CDS             782..>2148
                     /locus_tag="PLE93_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNH86728.1"
                     /translation="MNEATGTAPGRERASRIAIVGGGASGTLVAANLLRMAEVPLEIE
                     LIDESGDSGPGIPYRTPNPEHRLNVPACRMSAWADDPDHYLNWRRSRDPEAAPEEYTA
                     RSIYGEYLQELLETAATKAPPFATLGRLTDRVAGIFFADGRPVLRFADRQLEVDQVVI
                     ATGPMPGGDPVPVPEALKDRGLYLPSAWDTEAVETAGKDEEVLIIGTGLTMVDVALTL
                     GAAPDGPRIHAISRSGMFPKTHLDRLTRLSTPQIPPEGPVSKRELLGAFAEQLSLASA
                     RGDDWRDAIDSMRLVTGDAWRRMSVEDRRWLLENVNRPWEVHRYRMAPSVGAAFHRLI
                     DSGRVEIQQARVGAIEESGSRVRVELVGPKRSESREYDRVISACGACTNIRRDAPEPV
                     RGLIDSGHLRPDDLLLGVEVDRDGAARDARGDRSATISVLGSLRRGVDWESIGVTELK
                     TQSRD"
BASE COUNT          375 a          711 c          693 g          369 t
ORIGIN      
        1 gccccgcctc atcgagagga aggttgcgtc tgcgtggcgg gaggcctcga ccgcgatgtc
       61 ggtcgcagag ttgccgatcc cgagcaccag cacccgcttg ccttcgaaga tctccggggt
      121 ccggtagtcg tgggcgtgca tctgttcgcc ctcgaaggtg tccgaaccgg gaaaggcggg
      181 ttccggccag cgcttgtccc agtggtggcc gttgcagacc attacatcgc tgaaatggtc
      241 ggtgccgctc tcgccctcac ggttctgcca ggtcacttcc cagccgcccc cttcggcggg
      301 cacgaccgac tcgacctcgg tctgaaagcg gatcgaactc ctcaggccga aatggtcgac
      361 gaaatcctcg aagtaactgg cgatctggaa gtggtccgga tagtccggga aatcctccgg
      421 catcgggaac gccttgtact ccatcatctg ccgtgaagtg ttgatgtgaa gcgacttgta
      481 ggcggcagac tggccgttgt cattgtcgta cttccagttg ccgccgatcc aggatccctt
      541 ctcgaagcag acgaaatcaa tccccttctc ctgaaggctt ctcatcgcgg tgatgcccga
      601 ggacccggcg ccgatcacgc agacccgcgg cccgcccccg ccggacgggc cggctccgtt
      661 catctccgcc tgtctgcccg tctccactcc tgaagcttcc attcgggcag tgtagccaat
      721 acttgacatg gcttcgtgtc agtttgccct cactaggctc ctgactgatg ggaaagtccg
      781 gatgaacgaa gcaaccggga ccgcacccgg ccgggagcgg gcgtcccgga tcgccatcgt
      841 cgggggcggc gcttccggaa ctctggtggc cgcgaacctg cttcgaatgg ccgaggtgcc
      901 gctggagatc gaactgattg acgaatccgg cgattccggt cctggcatcc cctaccggac
      961 ccccaacccc gagcatcgtc tcaacgtgcc cgcctgccgg atgagcgcgt gggcagacga
     1021 tccggaccat tacctgaact ggcgccggtc ccgcgatccc gaggcggccc cggaggaata
     1081 cacggcccga tccatatacg gcgagtacct gcaggaactg ctcgaaactg ccgcgacgaa
     1141 agcccctccg ttcgccacac tcggccggct gaccgaccgg gttgccggca tcttcttcgc
     1201 tgacggaagg cccgtccttc gattcgccga tcggcagctc gaggtggatc aagtggtcat
     1261 cgcaaccggg ccgatgcccg ggggcgaccc ggttccggtc cccgaagcgc tgaaagacag
     1321 ggggctctat ctacccagtg cctgggacac cgaggcggtc gaaacagccg gcaaggacga
     1381 agaggtactg atcatcggca ccgggctgac catggtcgac gtcgcgctca ctctcggcgc
     1441 cgcacccgac ggacctcgca ttcacgccat ctcccgcagc ggcatgttcc ccaagaccca
     1501 cctggatcgc ctgacccgcc tttccacccc gcagataccg cccgaggggc cggtgtcgaa
     1561 gcgcgaattg ctgggggcgt ttgccgagca actgtcgctg gcctcggccc ggggcgacga
     1621 ctggcgcgac gcgatcgatt caatgcgact ggtcaccgga gacgcctggc gacggatgtc
     1681 cgttgaagat cgaaggtggc tgctggaaaa cgtgaaccgt ccgtgggagg tccaccgcta
     1741 ccggatggct ccgagcgtag gggccgcgtt ccaccggctg atcgactcgg gaagggtgga
     1801 gatccagcag gcgagggtcg gtgcgatcga agaatccggg tcgcgggtca gggtcgaact
     1861 ggtcggccca aagcgaagcg agtccaggga atacgaccgg gtgatctcgg cctgcggagc
     1921 ctgcacgaac atccgccggg atgccccgga gccggtcagg gggctgatcg attcgggcca
     1981 tctgcgtccc gacgacctcc tgctcggcgt cgaagtagac cgcgacgggg cggcccggga
     2041 cgcccgtggc gatcgctcgg cgacgatcag cgtgctgggc tcgcttcgcc gtggcgtcga
     2101 ctgggaatcg atcggggtga ccgaactcaa gacccagtcc cgtgacat
//
LOCUS       DAOQFI010000337         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30173_length_2142_cov_3.220891, whole genome shotgun sequence.
ACCESSION   DAOQFI010000337 DAOQFI010000000
VERSION     DAOQFI010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30173_length_2142_cov_3.220891"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PLE93_06490"
     CDS             complement(<1..172)
                     /locus_tag="PLE93_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNH86729.1"
                     /translation="MSNRLAEETSPYLLQHKDNPVAWQPWGPGALAEAQEREVPILLS
                     VGYAACHWCHVME"
     gene            230..1030
                     /locus_tag="PLE93_06495"
     CDS             230..1030
                     /locus_tag="PLE93_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86730.1"
                     /translation="MGMEASIFSRFWHDQIETNHQEAIFLLFVGFLLSFAFIRMSTRL
                     MRSPKVPWWPGSIVSEGGLHVHHHVFGIVLMMISGAIAFALDGGGTAFNLCGLAFGVG
                     IGLTIDEFALWLHLEDVYWSEEGRRSIDATVLTALVLALLMFGVRPFEIGSDSIGVLI
                     GSIITALFVFLVVAICFLKERVMHAFVGFFVWPVALYGACRLGKPGSPWAKRRYSERN
                     PSKQKRAEARFRPGRRTDRFKEKFRDLVGGGLIHPETDSPDDGSGTDT"
     gene            complement(1084..>2142)
                     /locus_tag="PLE93_06500"
     CDS             complement(1084..>2142)
                     /locus_tag="PLE93_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH86731.1"
                     /translation="STHFAAPTNDSIEVAEELKKRFGLPHWRFINSGTEATMDAVHLA
                     RGATGRDMILKVEGSYHGHHDAVMVSVAPPLDQIGTREDPISVPYGEGTPKALTDLTH
                     TVPFNNVDALKAVLENIGDQVAGMIVEPAMMNINIIPPKDGYLQAVRELTEQYGIKLI
                     FDEVKTGATMSHGGATGYYGVTPDMITLAKATCGGYPGGSIGMSDEVAQLVEDDRVKQ
                     YGTFNGNPLVMAAAKAALTEVLTEQAYVELNEKNEYLKGRCQEIIDKYGLPAYTEGIG
                     AKGCVIFSPERLYEYRDYAEYVDAELSTLGWLYHMNNGIFMTPGVEEEWTLSVFHTEQ
                     DLDNYANAFEAFAQDVTA"
BASE COUNT          320 a          658 c          726 g          438 t
ORIGIN      
        1 gctccatcac gtggcaccag tggcaggcgg catatccgac cgaaagcagg atcggcacct
       61 ccctttcctg cgcctcggcc agcgccccgg gaccccaggg ctgccacgcg accgggttgt
      121 ccttgtgctg gaggaggtac gggctggtct cttcggcaag gcggttgctc attgacacat
      181 gatgggggtt cggacatgcg ggcgttggtc gtggcaggat tggggggcga tgggaatgga
      241 ggcgtcgatc ttcagcaggt tctggcatga ccagatcgag accaaccatc aggaagcgat
      301 cttcctgctg ttcgtgggct tcctgctttc cttcgccttc atccggatga gcacgcggtt
      361 gatgcgatcc ccgaaggttc cgtggtggcc gggcagcatc gtcagcgagg gcgggctaca
      421 cgttcatcac cacgtcttcg gcatcgtcct gatgatgatc tcgggggcga tcgccttcgc
      481 gctcgacggg ggaggcaccg cattcaacct ctgtggcctc gccttcgggg tcggcatcgg
      541 tctgaccatc gacgagttcg cgctctggct tcacctcgag gacgtctact ggtccgaaga
      601 gggccgccgc tcgatcgacg cgaccgtcct gaccgcgctg gtcctggcgc tgctcatgtt
      661 cggggtccga cctttcgaga tcggttcgga ctcgatcggc gtcctgatcg gcagcatcat
      721 caccgccctt ttcgtctttc ttgtggttgc gatctgcttc ctcaaggaga gggtgatgca
      781 tgccttcgtc gggttcttcg tctggccggt cgccctttac ggcgcctgcc ggctgggcaa
      841 gcccggctcg ccgtgggcga aacgccgcta ctcggaaagg aatccctcaa agcagaagcg
      901 ggccgaggcg cgctttcgcc ccggccgccg caccgaccgg ttcaaggaga agttccgcga
      961 tctggtcggc ggcgggctga tccaccctga aaccgactcc ccggacgacg ggtcgggtac
     1021 cgacacctga agacggccgg aggctggccg gccggcgatc aatcgaccgc cggccgcgcc
     1081 tgatcaggcg gtgacgtcct gcgcgaacgc ctcgaacgcg ttcgcgtagt tgtcgaggtc
     1141 ctgctcggtg tggaagaccg aaagggtcca ctcttcctcg acgccgggag tcatgaagat
     1201 gccgttgttc atgtggtaga gccagcccag ggttgagagc tcggcgtcca cgtactcggc
     1261 gtagtcgcgg tactcataga gccgctcggg cgagaagatc acgcagccct tggcgccgat
     1321 gccctcggtg tacgcaggca ggccgtactt gtcgatgatc tcctggcagc ggcccttgag
     1381 gtactcgttc ttctcgttca gctcgacgta ggcctgctcg gtcaggacct cggtcagggc
     1441 ggccttcgcg gctgccatga cgagtgggtt gccgttgaag gtgccgtact gcttcacgcg
     1501 gtcgtcctcg accagctggg cgacctcgtc cgacatgccg atcgagccgc ccgggtaacc
     1561 gccgcacgtc gccttggcga gggtgatcat gtccggggtc acgccgtagt agccggtggc
     1621 gccgccgtgt gacatggtcg cgccggtctt gacctcgtca aagatcagct tgatcccgta
     1681 ctgctcggtc agctcgcgaa cggcctggag gtaaccgtcc ttcggcggga tgatgttgat
     1741 gttcatcatc gccggttcga cgatcatccc ggcgacctgg tcgccgatgt tctcgagcac
     1801 ggccttcagc gcgtcaacgt tgttgaaggg gacggtgtgg gtgaggtcgg tcagcgcctt
     1861 cggggtgcct tcgccgtagg gaacggagat tggatcctcg cgggtgccga tctggtccag
     1921 gggcggggcg accgagacca tcaccgcgtc gtggtggccg tggtaggagc cctcgacctt
     1981 gaggatcatg tcgcggccgg tggcgccgcg ggctaggtgg acggcgtcca tggtcgcttc
     2041 ggtccccgag ttgatgaaac gccaatgggg aagcccgaag cgcttcttca gttcttccgc
     2101 tacttcgatc gagtcattgg tcggggcggc aaaatgggtg ct
//
LOCUS       DAOQFI010000338         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30272_length_2138_cov_2.156985, whole genome shotgun sequence.
ACCESSION   DAOQFI010000338 DAOQFI010000000
VERSION     DAOQFI010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30272_length_2138_cov_2.156985"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..521)
                     /locus_tag="PLE93_06505"
     CDS             complement(102..521)
                     /locus_tag="PLE93_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86732.1"
                     /translation="MFMLEEKLIPEVATRPLPDLPTQGHVIARQAIGHLDPLTGLVLL
                     DRLVDRSAHGAEKFGQAWRTRDNCQEMCEETTDAVVYGVQETARIHQGLTPLDPDGEQ
                     ANTAREHLAIAVRYAALADHFAQLAGAEINGQQPGRK"
     gene            complement(525..1622)
                     /locus_tag="PLE93_06510"
     CDS             complement(525..1622)
                     /locus_tag="PLE93_06510"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="HNH86733.1"
                     /translation="MSPVAEQARTQTVTTVRLEDGRRITEHLTPDRQAKLYGLVVHGD
                     QLGMVEAVRAERPEGGKRLKFPHRPEDQGDLWLPADNHDALAKLVTEFRDQYELFVTP
                     ATFGEMIPGNHGVSGCQVVWIDQDDPEKIDILRKFPYRPHMVVATGGSGGVHAFWRLT
                     YPVGRDELGVMNRKLVHRLEGDRAAHNPARILRIPGSRNMKENTGEGADGICRIIYAD
                     LWRPPYEAEELVDGLTDYKQPAKPRRRNQFPNGQIKSANAAGWIKETVEVAEGSSPSD
                     YFYRLTGQPVATRGGHVRCPFTDHEDRNPSTHVYGDVGAGWFCFSCQRGGGPFHLAAA
                     LNGWKGGELTGDEFKNAAATLAAAYGIERKD"
     gene            complement(1619..1981)
                     /locus_tag="PLE93_06515"
     CDS             complement(1619..1981)
                     /locus_tag="PLE93_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86734.1"
                     /translation="MENTTPYSAKLQVKRVHIADVTIQGKTERLTWIRTGGLSVNILI
                     TETVVGNKLTAPRVIADFTVDETEEEVKAMCRDYVRRQNIPHLDQLPMRRLTVDDQRP
                     DEEIEFDLAKEPLFGAPR"
BASE COUNT          307 a          627 c          728 g          476 t
ORIGIN      
        1 gttggtgatg atcagtgccg cggcctcgta ggaggggtcg ttgaacgcgt cggtggcgag
       61 cagatcgctg tcgatcgccg cctcggtgtt gaccccggtg ctcattttcg acctggctgc
      121 tggccgttga tttcagcgcc tgcgagctgc gcgaaatggt ctgcgagggc cgcgtacctg
      181 accgcgatcg caagatgttc ccttgcggtg ttggcctgtt ctccatccgg atccagcggg
      241 gtgaggccct gatggatgcg ggcggtttcc tggaccccgt agacgacggc gtcggtggtt
      301 tcctcgcaca tctcctggca gttgtctcgg gtgcgccagg cctggccgaa cttctcggca
      361 ccgtgggcgc ttcggtcaac cagccggtcc agcaaaacca gcccggtgag cgggtcgaga
      421 tggccgatcg cctgccgggc aatcacatgg ccttgggtgg ggagatctgg aagcggccgg
      481 gtggctactt ccgggatgag tttctcttcg agcatgaaca ttgttcagtc ctttcgttcg
      541 atgccgtagg cggcggcaag cgtcgcggcg gcattcttga attcgtctcc ggtgagctcc
      601 ccgcccttcc agccgttgag ggctgcggcc aggtggaacg gccctccccc gcgctggcag
      661 gaaaagcaga accacccggc tcccacgtcg ccgtaaacgt gggtggacgg gttgcggtct
      721 tcgtggtcgg tgaacgggca tctgacatgc ccgccgcggg ttgcgaccgg ttgcccggtc
      781 agccggtaga agtagtcgct tgggcttgaa ccttcagcca cttcgactgt ttctttgatc
      841 cagccggcag cgttggcgct tttgatctgg ccgttgggga actggtttct gcggcgaggc
      901 ttggccggct gcttgtagtc ggttagcccg tcaaccagct cctcggcctc atacggtggc
      961 ctccacagat cggcgtagat gatccggcag attccgtccg caccttcccc ggtgttctct
     1021 ttcatgtttc gggagccggg aatgcggagg atccgggcgg ggttgtgtgc cgcccggtcg
     1081 ccttcgagtc gatggacaag cttgcggttc atgactccga gctcgtcgcg gcctaccggg
     1141 taggtgagcc gccagaaggc gtgtaccccg ccggagccgc cggtggcgac aaccatgtgg
     1201 gggcggtacg ggaacttgcg gagaatgtcg attttctccg ggtcgtcctg gtcaatccag
     1261 acgacctggc agccggaaac accatggttt ccggggatca tttctccgaa cgtggccggg
     1321 gttacgaaca gttcgtattg gtcacggaac tcggtgacga gtttcgcgag cgcatcgtga
     1381 ttgtcggccg gcagccagag gtcaccttgg tcttccggac gatgcgggaa cttgaggcgt
     1441 ttgcctcctt ctggccgttc ggcccggacc gcttcgacca tgccgagctg atcgccatga
     1501 acgaccaggc cgtaaagctt ggcctgccgg tcgggggtga gatgctcggt gatcctgcgg
     1561 ccgtcctcga gccgcaccgt tgtgacggtt tgcgtgcggg cttgttcggc gaccgggctc
     1621 atctcggggc gccgaacagc ggttccttgg cgagatcgaa ttcgatttcc tcgtcgggcc
     1681 gctggtcgtc gacggtgagt cgacgcatcg gcagctggtc gaggtgagga atgttttgcc
     1741 ggcgaacgta gtcgcggcac atcgccttca cttcttcttc ggtctcgtca acggtgaagt
     1801 cggcaatcac ccttggcgcg gtgagcttgt tgccgaccac cgtttcggtg atcagaatgt
     1861 tcaccgagag tcccccggtg cgaatccagg tgagcctttc ggtcttgccc tggatggtga
     1921 cgtccgcgat gtggacgcgc ttgacttgga gtttcgccga gtacggcgtc gtgttctcca
     1981 tcatgaatct ccttgctttt tgcgaaacgg cacgtagccg ctccgcacag gcatttggtg
     2041 cgcctgagcg gggataaccc ggccctcagc gcacccggat agggggcccc gtgtcggggc
     2101 cggttggatc cgggagtggt gggtcttgga gatggccc
//
LOCUS       DAOQFI010000339         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30512_length_2129_cov_2.694311, whole genome shotgun sequence.
ACCESSION   DAOQFI010000339 DAOQFI010000000
VERSION     DAOQFI010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30512_length_2129_cov_2.694311"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="argC"
                     /locus_tag="PLE93_06520"
     CDS             complement(<1..966)
                     /gene="argC"
                     /locus_tag="PLE93_06520"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNH86735.1"
                     /translation="MAEAAQPISPQGAKARVLVAGATGYAGALAAGLVWKHPRLELVA
                     VTGRSETGRKLSDLYPRYPVPLELKELEDSDFEGVEVGIVAYPHGAAAPVVAAMRGRG
                     LLVVDLSADFRFRDLPVYEEFYGPHGEPGLLDGAAYGLTELDREWIREAELVANPGCY
                     PTASILALAPLARAGLIEDVVIDAKSGVSGAGRGGGESTAFVTVTENVNAYKPAGHRH
                     RPEIVEKLGLIAAAGGNPEAAPDRLTFVPHLVPVDQGELVSCYVTPRRPVAQAELDAL
                     YTSAYADEPFIRLRDVPPGMRDVRETNFCDIFPLVTDDRILVESAI"
     gene            complement(959..>2129)
                     /locus_tag="PLE93_06525"
     CDS             complement(959..>2129)
                     /locus_tag="PLE93_06525"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNH86736.1"
                     /translation="LEMLGFGVEREGEDLLVTVPPDRYFDVTREIDLVEEVGRISDLD
                     RNLPATLPSGAGRPGGLSRQQALQRRAEDSLRESGFDEIVGWSFTDPGEKDRLRLAPE
                     DPRAQAVKLANPLSEDQSVMRTTLLGSVLGAARRNLSRGADRVALFESGRVYLPSGAF
                     GPGPLGGDFPGNTRPPSNEPQHLCALAVGSIDPSSWREPAAGSDFFALKGVLEHLAGG
                     LGTAVTVERIPGAPTQPFLHPGKSGVVAVGGQPIGWIGEIHPLVAADYDLDSAVAFEI
                     ALADLLQSSPVGEEAYEDFTPFPPVDRDLAVLVSDAEPSAGVLATVQQAGGELLTGVS
                     VFDVYSGEGVPEGEKSLALRLRFRAPDRTLSDEEIEPLWSGIIAALESAGGKLRG"
BASE COUNT          349 a          743 c          727 g          310 t
ORIGIN      
        1 gatcgccgat tcgaccagga tccggtcatc ggtgaccagc gggaagatgt cgcagaaatt
       61 ggtctcgcgc acgtcccgca tgcccggcgg cacgtcccgc aaacggatga acggctcatc
      121 ggcgtaagcc gaggtgtaga gcgcgtcgag ttcggcctgg gcaaccgggc ggcgcggggt
      181 cacgtaacag ctgaccagtt ctccctggtc gaccgggacc aggtgcggca cgaaggtcag
      241 gcggtcgggc gccgcttcag ggttgccgcc ggcagccgcg atcaggccga gcttctcgac
      301 gatctcgggc cggtgccggt ggccggccgg cttgtaggcg ttgacgttct cggtcactgt
      361 cacgaaggcg gtgctctctc ccccgccccg gccggccccg gaaacgccgg acttggcgtc
      421 gatcaccacg tcctcgatca gcccggcccg ggccagaggc gccagggcga ggatcgaagc
      481 ggtcgggtaa caaccgggat tggcgaccag ctcggcttcg cggatccatt cgcggtcgag
      541 ctcggtcagg ccgtaggcgg cgccatcgag cagtccgggc tcgccgtggg gtccgtagaa
      601 ctcctcgtag accggcaggt ccctgaaacg gaagtcggcc gagagatcga ccaccaggag
      661 gccccggccc cgcatcgcgg ccactaccgg ggcggcggcg ccatgcgggt aggcgacaat
      721 cccgacctcg accccttcga agtccgaatc ctccagctcc ttcagctcca gcggcaccgg
      781 gtagcgcggg tagaggtcac tcagcttccg gcccgtttcc gagcgaccgg tgaccgcgac
      841 cagttccagc cgcgggtgtt tccagaccag cccggccgcc agcgcgccgg cgtatccggt
      901 cgctccggcg accagcaccc gggctttcgc cccttggggg gagatcggct gtgcggcctc
      961 agccacgcag cttccctccg gccgattcga gcgcggcgat gatgcccgac cagagcggct
     1021 cgatctcctc gtcgctgagg gtccggtccg gcgcccggaa gcgcagcctc agggcgaggc
     1081 tcttctcgcc ctcgggaacg ccttcgccgc tgtaaacatc gaaaacggag accccggtga
     1141 gaagttcccc gccggcctgc tgaaccgtgg ccaggacgcc ggcggaaggt tcggcgtcag
     1201 aaaccagcac cgccaggtca cggtcgaccg gcgggaaggg tgtgaagtcc tcatatgcct
     1261 cctcgccgac cgggcttgac tgaagcaggt cggcaagggc gatctcgaag gcgaccgccg
     1321 agtcaaggtc gtaatcggca gcgaccaggg gatggatctc gccgatccag ccaatcggct
     1381 gacccccgac cgcgaccact cccgatttgc ccggatggag aaagggctgc gtcggagcgc
     1441 ccgggatccg ctccacggtc accgcggtgc caagccctcc ggccaggtgt tcaagcacgc
     1501 ccttgagagc gaagaaatcg gatcccgcgg ccggttcgcg ccaggacgaa ggatcgatcg
     1561 aaccaactgc cagcgcacac aaatgctgtg gctcgttcga agggggcctg gtgttgccgg
     1621 ggaaatcgcc cccgagcggg cccggtccga acgctccgga gggcagatag acccgcccgg
     1681 attcaaagag cgcgacccgg tcggctcccc gcgaaaggtt ccgccgggca gcgccgagca
     1741 ccgagccgag cagcgtggtc cgcatcaccg actggtcctc ggagagcgga ttggccagct
     1801 tcacggcctg ggcccgcgga tcctccgggg ccagcctgag ccgatccttc tcgccggggt
     1861 cggtgaagct ccagccgacg atctcgtcga agcccgactc gcgcagggaa tcctcggccc
     1921 gccgctgcag cgcctgctgg cggctgagcc cgccgggcct gccggcaccg gatggcaggg
     1981 tggcaggcag gttgcggtcg aggtcgctga tccggccgac ctcttcgacc aggtcgattt
     2041 cccgggtcac gtcgaaatac cggtcgggcg gtaccgtgac cagcaggtct tcgccttccc
     2101 gctcgacccc gaagcccagc atttcaagc
//
LOCUS       DAOQFI010000340         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30518_length_2129_cov_2.431533, whole genome shotgun sequence.
ACCESSION   DAOQFI010000340 DAOQFI010000000
VERSION     DAOQFI010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30518_length_2129_cov_2.431533"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLE93_06530"
     CDS             complement(<1..661)
                     /locus_tag="PLE93_06530"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HNH86737.1"
                     /translation="MKNQKRVEELRELIDQESEGPVLSVFCRTDPRDPANSSETPAWA
                     IALKNGLNHVAGFHEGNHGKEQTVKKLCAEAERRINGASAADRGRSVALFIGGDGKVD
                     SFTTFQIPVREDYVTVDSGPVVWPMLDVIDRGQRTGLVLLSSDRIRLLEWWDGRTRDL
                     EESTFDLELGDWHQYRGPARPQGTVGGGGVSNASAYDDRVEVWRARFVKAAAKAIAES
                     AS"
     gene            complement(773..1477)
                     /locus_tag="PLE93_06535"
     CDS             complement(773..1477)
                     /locus_tag="PLE93_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86738.1"
                     /translation="MPIDNRPFPRFVADASRHGIPHGRFAERLATAFKDACREIDDLP
                     AGTEIPEEFTWFPERAWSGRVWVPVSADGEAVLEENSEPEPIEFFGFVSFVQPEGGDP
                     TDFKASADFTDVVAADNPDWTIDISDEVIGTWLGEQGREAAMTLVWGRALVRDAFAVT
                     AELDDVTVDQDPLFGGQGGRADRFTLIAPDALKNYGDDAYLEIKLWTSRGKQVARESL
                     YEIEEPQTAPEQEPAE"
     gene            complement(1491..>2129)
                     /locus_tag="PLE93_06540"
     CDS             complement(1491..>2129)
                     /locus_tag="PLE93_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86739.1"
                     /translation="HLASLAELEEQSLAAAVAAWRSRMRAHSDSAYVQLILNQGPDSG
                     ASLEHSHAQLGAMEFVPPSVARERERVGAYRERTAGASLLGEILREEVRRGERLVAID
                     EEVALICPWASRHPYEMRLVPRRPVARFEEDEGGAAMLGRAIRALTELFGNPCQLNLW
                     VRTAPRGVEHFHWHLDLIPRLEPASAFEMATGVDINIVSPETAAETLRDAID"
BASE COUNT          342 a          701 c          725 g          361 t
ORIGIN      
        1 cgctggcact ttcggcgatg gccttggcgg ccgccttgac gaagcgggcc cgccagacct
       61 ccacccggtc gtcgtaggcc gaggcgttcg agacgccgcc accaccgacc gtgccctggg
      121 gccgggccgg gccccggtac tggtgccagt caccgagttc gaggtcgaag gtcgattctt
      181 cgagatcccg ggtgcggccg tcccaccatt cgagcagccg gatccggtca ctcgagagca
      241 ggaccaggcc ggtgcgctgg ccgcggtcga tcacatcgag catcggccag accaccgggc
      301 ccgagtcgac cgtcacatag tcctcgcgaa ccgggatctg gaaagtcgtg aacgagtcga
      361 ccttcccgtc accgccgatg aagagcgcga cgctgcgccc gcggtcggcg gccgaggccc
      421 cgttgatccg ccgttcggct tcggcacaga gcttcttcac cgtctgctcc ttgccgtggt
      481 ttccctcgtg aaaacccgcc acgtggttaa ggccgttctt caaggcgatc gcccaggccg
      541 gggtctcgct gctgttggcc gggtcccgag ggtcggtccg gcagaagacc gaaagcaccg
      601 gcccctcgga ttcctggtcg atcagttccc gaagctcctc aacccgcttt tggttcttca
      661 tggaaccaaa atacctgcat gcattccggt cttcgccgga atgccagtaa aacccgcccg
      721 gtcacgacat ccacaaatgg atgaccgggc tccatcggtg gctattcggt ggctattcgg
      781 cgggttcctg ttccggtgct gtctgcggtt cttcgatttc gtagagggat tcccgtgcga
      841 cctgtttgcc gcgggaggtc cagagcttga tctcgaggta ggcgtcatcg ccgtagttct
      901 tgagcgcgtc gggagcgatc agggtaaagc ggtcggcgcg accgccctgg ccgccgaaaa
      961 ggggatcctg gtcaacggtg acatcgtcga gctcggcagt caccgcgaag gcgtcacgga
     1021 ccagggcccg gccccagacc agggtcatcg ccgcttcgcg gccctgctct ccaagccagg
     1081 tgccgatcac ttcatcggag atgtcgatcg tccagtcggg gttgtcggcg gcgaccacgt
     1141 cggtgaagtc ggccgaggcc ttgaagtcgg tcggatcgcc gccctccggc tgaacgaagc
     1201 tgacgaagcc gaagaactcg atcggctcgg gttcgctgtt ttcctcgagg accgcttcgc
     1261 cgtcggcgga aaccggaacc cagacccggc ccgaccaggc ccgctccgga aaccaggtga
     1321 actcttccgg gatctccgtt ccggccggca ggtcatcgat ctcgcggcag gcgtccttga
     1381 aagcggtagc cagccgctcg gcaaagcggc cgtgaggaat gccgtgacgg gaagcatcgg
     1441 caacgaaacg ggggaagggg cgattgtcga tcggcatcgg acgagcctac tcagtcgatc
     1501 gcgtcgcgga gagtctcggc ggcagtttcg ggactgacga tgttgatgtc cacgccggta
     1561 gccatctcga aggcggaggc cggttcgagc cgggggatca ggtcgaggtg ccagtggaag
     1621 tgctcgaccc cgcggggtgc ggtgcggacc cagagattga gctggcaggg gttgccgaag
     1681 agttcggtca gcgcccggat cgcccggccc agcatcgccg ccccgccttc gtcttcctcg
     1741 aagcgggcga cgggccggcg cgggacgagt cgcatctcgt aggggtgacg ggaggcccac
     1801 gggcagatca gggcgacctc ttcatcgatc gcgaccagcc gctcgccacg gcggacttcc
     1861 tccctcagga tctcgccgag caggctggcc ccggcggtcc gttcccggta ggccccgacc
     1921 ctttcgcgtt cgcgggcgac cgatggcggc acgaactcca tcgcgccgag ctgggcgtga
     1981 ctgtgctcca gcgaagcgcc ggagtcgggg ccctggttga gaatcagctg cacgtaggcc
     2041 gaatccgaat gggcccgcat gcggctgcgc caggcggcga ccgcggcggc cagcgactgc
     2101 tcctcgagct cggccagcga ggcgaggtg
//
LOCUS       DAOQFI010000341         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30520_length_2129_cov_2.095950, whole genome shotgun sequence.
ACCESSION   DAOQFI010000341 DAOQFI010000000
VERSION     DAOQFI010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30520_length_2129_cov_2.095950"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..658
                     /locus_tag="PLE93_06545"
     CDS             242..658
                     /locus_tag="PLE93_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNH86740.1"
                     /translation="MSVLTPEATRLIESDALAHLVTLNRDGSPQLSCVWVGLDGDEIV
                     SGHMMKRLKLKNVERDPRIVLSIESPSANEMGLKEYLVVHGSARVEEGGAAALLQRLA
                     HIYMGPDAVFPPPGIDAPGWVLRITPERLGGVGPWA"
     gene            759..>2129
                     /locus_tag="PLE93_06550"
     CDS             759..>2129
                     /locus_tag="PLE93_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86741.1"
                     /translation="MGAHDRHVGGMMAAKSGGGVRSAGLAALVILLLFAGVSLAPAKA
                     SAADSVYIDYPPQGGIYQAGPYDYIGRAFPDIFPLPPLYLPWVRGAVQEETSPGVWGG
                     PSCPGSLQALPDLCGIYRFRTLLDSFGEDWKASDSEADGLAGSPSLTDGKYRIKVGQA
                     GAFGLPDIYAENYFFVDGTKPDSSIDVGPSPFSNDASANIHFQFSGTDPVVNDYASGI
                     DYFNCKLDTGPWDHCTPPGGSAPFTYDLSAPEGGHTLYVQAVDHANNEDDTAATHSWI
                     IDETPPDITITKPVNKDRYLLHHGPAPVFNCTDPLAGTPPAASGIASCTATPINDEDL
                     GPHYFTVTAVDKAGNTSTKTVAYTIDPPDYGDFIGEDHPLAYYRLDEAVGSDDMIDRS
                     GNHHDGIYQNGIALGRNGATACERRPHPPRVCELAAPAENKAGYFPARDGHGYVNGIT
                     APTTGYT"
BASE COUNT          369 a          717 c          667 g          376 t
ORIGIN      
        1 acctggatcc gggccgcgtc gatgatcgtc gtcgtgtctg cgtgcgggtt gtcgtggacc
       61 gccgaaagga cggccatccg gggctgggtc acgcgaagct cggcgccgcg cagcaggttc
      121 tcgtattcga cggtcgaagg caccgccggt agttatacac cttttcttga accgatcaag
      181 ttaaggcctg ccggggccta atcgatgtcc gtgggtccgg gggtcggcta ggctcggttt
      241 gatgagtgtg cttacgccgg aagcgacgcg gttgatcgaa tcggatgccc tggcccacct
      301 ggtcaccctg aaccgcgacg ggagccctca gctttcctgc gtctgggtcg ggctcgacgg
      361 cgatgagatc gtcagcggcc acatgatgaa gcggctgaag ctgaagaacg tcgagcggga
      421 tccgcggatc gtgctttcga tcgagtctcc ttccgccaac gagatgggct tgaaggagta
      481 tctcgtggtc cacggcagcg cccgcgtcga ggagggcggg gcagcggcgc tgcttcagcg
      541 tctggctcac atatatatgg gtcccgacgc ggtctttccg cctccgggaa tcgatgcgcc
      601 cggctgggtg cttcgcatca ccccggagcg cctcggcggc gtcggcccct gggcctgact
      661 ccgacggtcg aggtccgggt tgtcataact cgagaaattc ggcttcctgg caagaaattc
      721 gtgcaggatc gaacactgtt tggtaaggtc gcaagtgtat gggggcacac gacaggcatg
      781 tgggtggaat gatggccgcg aaaagcgggg gcggtgtccg gtcggccggc cttgccgctc
      841 tggtgatcct gctgctcttt gcaggcgttt ccctggcccc cgccaaggcg agcgcggccg
      901 actcggtgta catcgactat ccgccgcagg gcggcatcta ccaggcgggt ccgtacgact
      961 acatcggccg ggcgttcccg gacatcttcc cgcttccgcc gctctatctg ccctgggtga
     1021 ggggcgcggt ccaggaggag acgtccccgg gagtctgggg cggcccttcc tgcccgggtt
     1081 cgctccaggc acttcccgat ctttgtggca tctaccgttt ccggaccctc ctcgactcgt
     1141 tcggcgagga ctggaaagcc tctgacagcg aggccgacgg cctggccggt tcgccctcgc
     1201 tgaccgatgg caagtaccgc atcaaggtcg gtcaggccgg agccttcggg ttgccggaca
     1261 tctacgccga gaactacttc ttcgtcgacg gcaccaagcc ggactcttcc atcgacgtcg
     1321 ggccgagccc cttcagcaac gacgcctcgg ccaacattca cttccagttc agcggtactg
     1381 atccggtcgt aaatgactac gcgagcggca tcgactactt caactgcaag ctcgacacgg
     1441 gcccgtggga tcactgcacc ccgcccggtg gctcggctcc cttcacctac gacctgagtg
     1501 cacccgaagg cggccacacg ctctacgtcc aggcggtcga tcacgccaac aacgaggacg
     1561 acaccgccgc cacgcactcg tggatcatcg acgagacacc ccctgacatc accatcacca
     1621 agccggtcaa caaggaccgg tacctgctgc accacggtcc cgctccggtc ttcaactgca
     1681 ccgaccccct cgccgggacg cccccggccg cgagtggcat cgccagctgc accgccacgc
     1741 cgatcaacga cgaagatctt ggtccgcact acttcacggt gacggcagtc gacaaggcag
     1801 gcaacacctc gaccaagacc gtcgcctaca cgatcgaccc gccggactat ggggatttca
     1861 tcggcgagga ccatccgctc gcctactacc gccttgatga agcggtcggt tccgacgaca
     1921 tgatcgaccg gtcgggcaac caccacgacg ggatctacca gaacgggatc gcgctgggac
     1981 gaaacggcgc caccgcctgc gagcgtcgcc cgcatccgcc gcgcgtctgc gagcttgcgg
     2041 caccggccga gaacaaggcc ggctacttcc cggcccgtga cggtcacggt tacgtgaacg
     2101 ggatcacggc cccgaccacc ggttacacg
//
LOCUS       DAOQFI010000342         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30690_length_2122_cov_3.466860, whole genome shotgun sequence.
ACCESSION   DAOQFI010000342 DAOQFI010000000
VERSION     DAOQFI010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30690_length_2122_cov_3.466860"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLE93_06555"
     CDS             <1..1080
                     /locus_tag="PLE93_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNH86742.1"
                     /translation="AETEPLERVELDLDSPGEGELLIRIRAAGLCHSDLSVITGQRPR
                     PMPMALGHEAAGEVVATGPGDHGFVEGDHVVLAFVPACGECRFCAQERPALCVPGGEA
                     NVEGTLLGGHIRLFEESGTEVHHHLGVSALSDHAVVSARSAVKVPDDLPFDIAALFGC
                     AVLTGAGAVLNTGAAALPARSVAVFGLGGVGLAALLAAKSQNPETLVAVDTVPAKLEL
                     ARELGADLTIQAGPDLVDSIREANGGGVDLAIETVGNENVLADAYAATGRGGTTVTAG
                     LPHPSRKLEIPAISLVAEERTLKGSYLGSCVPRRDIPAFIRMYRDGELPVEKLLSHRL
                     TPDQINEGFDRLARGEAVRQIVIFD"
     gene            complement(1144..>2122)
                     /locus_tag="PLE93_06560"
     CDS             complement(1144..>2122)
                     /locus_tag="PLE93_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86743.1"
                     /translation="PHFEGKTTAETQYAKKWGLSMVVHDLRKVVNMARKGGRKVVLGG
                     HSLGGSIVTAYATWNFNGTPGAKGLSGLVFIDGAGAREAADLPTAGEAQANLDSLNAE
                     SASPFITLVPPLPWAAGVFNAMGSTAALQDPDSRSLMEDFPLLPDSLKPPVSATNLAQ
                     YAYAIDADTGPENLQLVQSHVGDLADSGDPRGWVNGQLGTAQRAAQVFTEIDGMDGTS
                     WYHPLRLSIDAGAINNGIATPAQQVYGIKTTMGKRVHLPMYSFDTSLGDGRVKRATDA
                     LAAQSKVPKSWVRTVVRKETYAHIDPLSATPSKNDFIKTLVPFLKKQVK"
BASE COUNT          318 a          721 c          718 g          365 t
ORIGIN      
        1 gccgagaccg aaccgcttga acgggtcgaa ctggatctcg attcgcccgg cgagggcgag
       61 ctgctgatcc ggatccgggc agccggactc tgccattccg acctttcggt gatcaccggc
      121 cagcggccgc ggccgatgcc gatggcgctc ggccacgagg cggccggtga agtcgtcgcg
      181 accggcccgg gcgaccacgg cttcgtcgag ggcgaccacg tggtgctcgc cttcgtcccg
      241 gcctgcggcg agtgccgctt ctgcgcgcag gagcggcccg cgctctgcgt tcccggcggt
      301 gaagccaacg tcgagggaac cctgctgggt ggtcacatcc gcctgttcga ggagagcggc
      361 accgaggtcc accatcacct cggcgtttcc gccctttccg accacgccgt ggtctcggcc
      421 cgctcggcgg tcaaggttcc ggatgacctt ccctttgaca tcgcggccct tttcggctgc
      481 gcggtcctga ccggtgccgg cgcggtgctc aacacgggtg ccgccgcttt gccggcccga
      541 tcggtcgccg tcttcggact gggcggggtc ggcctggcgg cgctgcttgc cgcgaagtca
      601 caaaacccgg aaaccctggt cgcggttgac accgtgccgg ccaagctcga gctggcccgc
      661 gagctgggag cggacctgac cattcaggcc gggcccgatc tggtcgattc gatccgcgaa
      721 gcgaacggcg gcggcgtcga cctcgcgatc gaaacggtcg gcaacgagaa cgtgctggcc
      781 gatgcctacg cggcaaccgg ccgcggcggc accacggtca ccgccggcct gccccacccc
      841 agccgcaagc tggagatccc ggcgatctca ctggtcgccg aggagcggac cctgaaaggc
      901 tcgtacctgg gctcctgcgt gccccgccgg gacatccccg cgttcattcg catgtaccgg
      961 gacggtgagt tgccggtcga gaagctgctc agccaccggc tcacaccgga tcagatcaac
     1021 gagggcttcg accgcctcgc ccggggcgag gcggtccgcc agatcgtcat cttcgactga
     1081 ggctgcaact ggattgaagt tattcgctcc atgtgcggat aacttcaatc cagatggcgg
     1141 cccctacttg acctgcttct tcaggaacgg gaccagcgtc ttgatgaagt cgttcttcga
     1201 cggggtggcg ctgagcgggt cgatgtgggc gtaggtctcc ttccggacca ccgtccgcac
     1261 ccagctcttc ggcacctttg actgggccgc aagcgcatcg gtcgcccgct tgacccggcc
     1321 gtcgccgagc gaggtgtcaa acgagtacat cggcaggtga acccgcttgc ccatggtcgt
     1381 cttgattccg tagacctgct gggccggggt ggcgatcccg ttgttgatcg caccggcgtc
     1441 gatgctcagc cgcagcgggt gataccagga ggtgccgtcc atgccgtcga tctcggtgaa
     1501 gacctgggcg gcccgctggg cggtgccgag ctggccgttg acccagcccc gggggtcacc
     1561 ggaatccgcg aggtcaccga cgtggctctg gaccagctgc agattctccg ggccggtgtc
     1621 ggcgtcgatc gcgtatgcgt actgggccag gttggtcgcc gaaacgggcg gcttcagcga
     1681 atccgggagc agcgggaaat cctccatcag cgagcgcgaa tccggatcct gaagcgccgc
     1741 ggtcgaaccc atcgcgttga agacccctgc cgcccagggc agcggcggca ccagcgtgat
     1801 gaagggcgag gcgctttcgg cgttcaagct gtcgaggttg gcctgcgctt ccccggccgt
     1861 gggcaggtcc gcggcctcgc gggccccggc accgtcgatg aagaccaggc cggaaagtcc
     1921 cttggcgccc ggcgttccgt tgaaattcca ggttgcgtaa gcggtgacga tcgaaccgcc
     1981 cagcgagtgg ccgccaagca cgaccttgcg gccgcccttg cgggccatgt tcacgacctt
     2041 gcgaagatcg tgaacgacca tgctcaggcc ccacttcttc gcgtactggg tttccgcggt
     2101 ggtcttgccc tcgaaatgag gc
//
LOCUS       DAOQFI010000343         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30694_length_2122_cov_3.008224, whole genome shotgun sequence.
ACCESSION   DAOQFI010000343 DAOQFI010000000
VERSION     DAOQFI010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30694_length_2122_cov_3.008224"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLE93_06565"
     CDS             complement(<1..372)
                     /locus_tag="PLE93_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH86744.1"
                     /translation="MKFRLMFGLFAAASLFALPNQAGAEISMNPMPGNPLTDTGQGGM
                     VRPVDLNDDGLDDLAIGSATAATGGVRIRIAGADGAWSPKPTLLAGSPVGDIVPADFD
                     QDGNEDLLVQISFPGLSANDQY"
     gene            complement(447..1601)
                     /locus_tag="PLE93_06570"
     CDS             complement(447..1601)
                     /locus_tag="PLE93_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNH86745.1"
                     /translation="MTLTCLLAIPASASAVEATADGNSVQLSDTPLEAAYTFDPGQVA
                     FGDVPVGSTASQLITLTNTGGSPALIRGVFAGKVGTDWLSFGISQDTCSPVPPGITRE
                     LPAGQSCSYLLEFTPSQRGGYAEITGVVTEGEVHFIPVGGNGTEPAGEFDTRSFDFGS
                     SPVGPSSNRPKHTFNLSSTGLDPVTVQSLSIGGPDQDAFELADPAVCSAPIPVNEKCQ
                     ITVTFDPESGSPGPRAATLTALTTGGVVEVALTGNATAPLTYRAKMQIKFPKKAVVGR
                     SARFKVTLTNTGTGPIAGAALEYKARQGKSGKAVVSKSVKVPALQSGRKTATVTVKLP
                     KNRLSRGKGKGLLVNFELVHQGLTLAARRGTTRLDFGQAKSRRPRDVSDT"
     gene            1787..>2122
                     /locus_tag="PLE93_06575"
     CDS             1787..>2122
                     /locus_tag="PLE93_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNH86746.1"
                     /translation="MSDSESPVVLTGTMNRLNGTEQDLGEYLGQVTLVVNTASKCGLT
                     PQFEALQAVYSDKKDEGFTVLGFPANDFMEQEPGSADEIAEFCQINYGVEFPMFEKST
                     VVGDEANPLF"
BASE COUNT          363 a          641 c          727 g          391 t
ORIGIN      
        1 gtactgatca ttggccgaca ggccggggaa gctgatctga accagaaggt cctcattgcc
       61 gtcttggtca aaatcagccg gaacgatgtc accgaccggt gagccggcca gcagggtcgg
      121 tttcgggctc caggcgccgt cggcgccggc aatccggatg cggaccccgc ctgtggcggc
      181 ggtggcagat ccaatcgcca ggtcgtccag gccatcgtcg ttgaggtcga ccggtcggac
      241 cattcctccc tgcccggtgt cggtcagcgg attccccggc atcgggttca tcgagatctc
      301 ggcccccgcc tgattcggca aggcaaaaag agatgcggcc gcgaacagcc cgaacatcag
      361 acgaaacttc atggtgttct ccctcaacct tctggttggc tttcccaagc aggagtgatt
      421 gtgacggacc ttgcggtcgc gggcttctag gtgtcggaga cgtcccgtgg ccgtcggctc
      481 ttggcctgac cgaaatcgag gcgggtcgtg cctcttcggg cggccagggt cagaccctgg
      541 tgaaccagct cgaagttgac cagcagtccc ttccccttgc cgcgggagag ccggtttttc
      601 ggcagcttga ctgtgaccgt cgcggtcttg cggccgctct gcaaggcagg gaccttcacg
      661 gatttgctga ccacggcctt gccgctcttg ccctgcctgg ccttgtactc gagtgcggcg
      721 ccggcgatcg gcccggtgcc ggtgttggtc agtgtcacct tgaatctggc cgacctgccg
      781 accacggcct tcttcgggaa cttgatctgc atcttcgccc tgtaggtgag gggcgcggtg
      841 gcgttgccgg tcagggcgac ctcgaccacg ccacccgtgg tcagggcggt cagggtggcc
      901 gcgcggggac cgggactgcc ggactccgga tcgaaggtga ccgtgatctg gcatttctcg
      961 ttgaccggga tcggggccga gcagacggcg ggatcggcca gctcgaaggc gtcctggtcc
     1021 ggccccccga tggaaagcga ctggacggtg accgggtcga ggccggtcga ggaaaggttg
     1081 aaggtgtgtt tgggccggtt cgacgagggg ccgaccggag agctgccgaa gtcgaagctt
     1141 cgggtgtcga actcgccggc cggctcggtg ccgttgcccc cgaccggaat gaagtgaact
     1201 tcgccttcgg tcacgacccc ggtgatctcg gcatatccgc cccgctgtga gggagtgaat
     1261 tcgagcaggt aggaacagga ctggccggcg ggaagctcgc gggtgattcc gggcgggacg
     1321 gggctgcatg tgtcctggga aatgccgaat gaaagccagt cagtgcccac ttttccggca
     1381 aagacccccc tgatcagggc cgggctccca ccggtattgg tcagagtgat caactgtgac
     1441 gcggtggagc cgaccggaac gtcgccgaag gcgacctggc cggggtcgaa tgtgtaggcc
     1501 gcctccaggg gggtgtcgga gagctgaacg gagttgccgt cggccgtggc ctctaccgcc
     1561 gaagcggatg ccgggatggc gagcaggcag gtcaacgtca ccagaactgc ggccagatga
     1621 ccccttgaca tcccttaccc ccaattgctc gttacctgaa aggaagatcc cctctgccga
     1681 ccatattcct ttttccggtc cggcgagccg gcctagttct tcccggttat ctgggaagga
     1741 tggaaaggct gaaagttgcc ggtgcggagc gggtacgatc ccgctcatga gcgatagcga
     1801 gagcccggtc gtcctgaccg gcaccatgaa ccggctgaac ggcaccgaac aggatctggg
     1861 ggagtacctg ggtcaggtga ccctggtcgt caataccgcc agcaagtgcg ggctgacccc
     1921 gcagttcgag gccctgcagg cggtctattc cgacaagaag gacgaggggt tcacggtgct
     1981 cggtttcccg gccaacgact tcatggagca ggaacccggc agcgccgacg agatcgccga
     2041 gttctgccag atcaactacg gggtcgagtt cccgatgttc gagaagtcga ccgtggtcgg
     2101 ggacgaggcg aacccgctct tt
//
LOCUS       DAOQFI010000344         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30806_length_2118_cov_2.810470, whole genome shotgun sequence.
ACCESSION   DAOQFI010000344 DAOQFI010000000
VERSION     DAOQFI010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30806_length_2118_cov_2.810470"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..1566
                     /locus_tag="PLE93_06580"
     CDS             385..1566
                     /locus_tag="PLE93_06580"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNH86747.1"
                     /translation="MPEAVIVDTVRTPIGRAFKGSLSQVRPDETGAFVIDQLLERNPG
                     VDPKAVEEVFCGVGMPQGKQAYNLARILVLLSDKLTQATTGVTVSRYCASSLDAIRHA
                     SNAVKNGEGDIYIAAGVEFVSQFNARTEIAGSIAAGEDDQNENLIGNNGQPNAYIDMG
                     LTAVNVANDHGVSREAMDDYALRSQHLAVASQESGFFDREIVPVTLPDGTVVSKDDGP
                     RADGGDMREKLASLPEAFGGGGVTAGNSCPLNDGAAAVLIMSDTKAKELGLTPRAKIV
                     TSATAGNEPEKMGVAPIWAVKKLLERANMSINDIDTVELNEAFAAQVIPISEEVGIPM
                     DKLNTHGGAIALGHPFGMTGARIMGTLLNVMETDDHQFGIETMCVAGGQGEAMLVERL
                     K"
     gene            complement(1713..1991)
                     /locus_tag="PLE93_06585"
     CDS             complement(1713..1991)
                     /locus_tag="PLE93_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNH86748.1"
                     /translation="MTDALACPKCGSEMRSYERNQVVVDQCTGCGGLFLDRGELERLV
                     EAESSFYSKPQSSAPPQQEHRDDRKYDDRGKGKKKKSKRSFLEDFLEF"
BASE COUNT          382 a          720 c          679 g          337 t
ORIGIN      
        1 cggtctgggg gtcgaaggtc tggccgggag tcccgggcgg ggcggccgcc tgaccccggg
       61 acgatccggc ctggccggga gcgggggcgg gctgcgaggg cgaaggccgg ccaggctgcg
      121 gtgaagcggg cggctgttgc gggcggccgg acccggctga agcgggtgcc ggacgaccac
      181 cggccggcgc ctgcggagtt ccttgcgggg cgtttatggg cacatctgaa gtgccgccat
      241 cctggggcga attgctcatc gccagtcaat ctacccgtcc cgcatttccg cagttcatta
      301 cgctgcttag tggcatttat ccgtcatgtc ccggccaggt gccctcacac ctcagctaca
      361 caaacccaag gagaaagact tcgcatgcct gaagcagtga ttgtggacac agtccgcacc
      421 ccgatcggac gcgccttcaa gggctcgctt tcccaggtcc gtcccgacga gaccggcgcc
      481 ttcgtgatcg accagctcct cgaacgcaac cccggtgtcg accccaaggc ggtcgaagaa
      541 gttttctgcg gcgtcggcat gccgcagggc aagcaggcct acaacctcgc ccgcatcctc
      601 gtcctgctct cggacaagct gacccaggcc accaccggcg tcaccgtctc gcgttactgc
      661 gcctcgagcc tcgacgcgat ccgtcacgcc tcgaacgcgg tcaagaacgg cgagggtgac
      721 atctacatcg ccgccggcgt cgagttcgtc agccagttca acgcccgcac cgagatcgcc
      781 ggttcgatcg ccgccggtga ggacgaccag aacgagaacc tgatcggcaa caacggccag
      841 cccaacgcct acatcgacat gggcctcacc gcggtcaacg tcgccaacga ccacggcgtc
      901 agccgcgagg ccatggatga ctacgccctg cgctcgcagc atctggcagt cgcctcccag
      961 gagtccggct tcttcgatcg cgagatcgtc ccggtgaccc tgccggacgg caccgtggtc
     1021 agcaaggacg acggcccgcg cgccgacggc ggcgacatgc gcgagaagct cgccagcctg
     1081 cccgaggcct tcggcggcgg cggcgtcacc gccggcaact cctgcccgct gaacgacggc
     1141 gccgcagccg tgctgatcat gagcgacacc aaggccaagg agctcggcct gacgccgcgc
     1201 gcgaagatcg ttacctcggc caccgccggc aatgagccgg agaagatggg cgtcgccccg
     1261 atctgggccg tcaagaagct cctcgagcgc gccaacatgt cgatcaacga catcgacacc
     1321 gtcgagctga acgaggcatt cgccgcgcag gtgatcccga tctccgaaga ggtcggcatc
     1381 cccatggaca agctcaacac ccacggtggc gcgatcgccc ttggtcatcc cttcggcatg
     1441 accggtgccc gcatcatggg caccctgctc aacgtcatgg agaccgatga ccaccagttc
     1501 ggcatcgaga ccatgtgcgt cgccggcggc cagggcgagg ccatgctggt cgagcgcctc
     1561 aagtagcggc accgcaacat cccgttgcat gcgaaagggc ccggcagatg ccgggccctt
     1621 tctcgttacc ggatcaatcc cgaggtgccg cacaaacagg cccatcacgg gccccaacgt
     1681 gcggcacctt ggctggtgcc gggttttcct gcctagaact cgaggaagtc ttcgaggaag
     1741 gagcgcttcg actttttctt cttgcccttg ccgcgatcgt cgtacttgcg gtcatcgcgg
     1801 tgctcctgct gaggcggcgc cgaggattgg ggcttcgagt agaaagaact ctcggcttcg
     1861 accaggcgtt cgagttcacc gcggtcgagg aagagtcctc cgcagccggt gcactggtcg
     1921 accacgacct ggtttcgctc gtaggagcgc atttcagatc cacacttggg gcaggcgaga
     1981 gcatcagtca tgaggcgaac ctaaccagcg ccctttcagc tttcttaaag ggacggtcaa
     2041 tcgaaaacgc gatcggcaat cagcccggcc agggcgaggg atgaggtggc cgccggggag
     2101 ggggcgttgc ggcgcgcg
//
LOCUS       DAOQFI010000345         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30836_length_2117_cov_2.585354, whole genome shotgun sequence.
ACCESSION   DAOQFI010000345 DAOQFI010000000
VERSION     DAOQFI010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30836_length_2117_cov_2.585354"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLE93_06590"
     CDS             complement(<1..714)
                     /locus_tag="PLE93_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86749.1"
                     /translation="MAMILNKKLTSSNIPLHSYSGIAESADLVPYCEYPRSLAELRQL
                     ALTFDLDRYREENPDLPETEPYLEQLRSEIDSGFVPEMSRLRAGLVLMHHWYGQFVSL
                     GAEEGKLIDEILDSLYRDVSGGSTRPVDGLGLHEPGIGYWPHGKGDTWHRRFVFEATR
                     PVDRQDLFSRVAGQLLKDGLSGVLIRGLYLQPPGYGDDPDGPVGHIFTASGEQETTDE
                     RDRSGTEGTPESSIETMIRE"
     gene            complement(722..>2117)
                     /locus_tag="PLE93_06595"
     CDS             complement(722..>2117)
                     /locus_tag="PLE93_06595"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HNH86750.1"
                     /translation="GIVGVVRNLAETIRSLDPQPDVSQVAGEVQRLLDRSVGAEEYVI
                     RAAADGADADALLDLNQIDFDALAARLAGKKRTAVQKLTGRLSGEIEAAAQRNPTRRS
                     LVERMRTLIDQYNSGSLNVDEMLRRLQVLSRDLSEEERRTVTEGLTEPELAIFDLLTK
                     PDPALTEDQEKAVKAVSKKLLDHIHEKLVLDWRMKAETREAARGMVKEILDELPDVYE
                     PELWEAKTGLVFDHIFASYYDDGSSVYAGDMPPAGQLSPGMASPDLPGAEIDVETVKS
                     DLLEKIRTDRAFAALVAEQLRGDEAFFAVETDDLIRGDETFEAEFKSTARWNLRDGCK
                     DKRMEDAVVKSIAGFLNADGGTLLIGVADDGTIIGLNHDVPLVKPPNADGFVNWLTTH
                     LINALSHSVAMRTKMRIDQFDEEQVCRVDVAASSVPVRARMSDGEEVFWVRMNNSTRA
                     FPESEADAYIRDRW"
BASE COUNT          383 a          666 c          600 g          468 t
ORIGIN      
        1 ttcgcggatc atcgtttcga tggacgattc gggcgttccc tcagttcccg atcggtcacg
       61 ttcgtctgtg gtctcttgct caccggaagc agtgaagatg tgcccgacgg gaccgtccgg
      121 gtcatctcca tagccgggcg gctgcaaata gagccccctg atcaggacgc cggagagacc
      181 gtccttcagc aactggcccg ccacccggct aaagaggtct tgcctgtcaa ctggtcgagt
      241 tgcttcgaat acaaatcgac ggtgccacgt gtcgcccttg ccgtgcggcc aataaccaat
      301 tcccggctcg tgaaggccga gaccgtcgac ggggcgcgta cttccaccgc tgacgtctcg
      361 atacagcgaa tcgagaatct cgtcaatcaa cttcccttct tcggcaccaa gactcacaaa
      421 ctgcccgtac caatggtgca tcagaaccag gccggccctg agccttgaca tttcgggaac
      481 gaaacccgag tcgatttcag atcgaagttg ctcaaggtac ggttccgttt ccggcagatc
      541 ggggttctcc tcgcggtacc gatccaggtc aaaggtcaag gccagttggc gaagttctgc
      601 cagcgagcgt gggtactcgc agtaggggac aaggtctgca gattccgcga ttcccgagta
      661 gctgtggagc gggatgttcg aggatgtcag cttcttgttc agaatcatcg ccatctgtcg
      721 ctcaccaacg gtcccggatg taggcatccg cttctgactc ggggaaggcg cgggtgctgt
      781 tgttcattcg cacccagaac acttcttcgc cgtcgctcat gcgggccctg actggcaccg
      841 aagaggctgc cacgtcaact cggcagactt gttcttcgtc gaattgatca atccgcatct
      901 tggttctcat cgcaaccgag tgactgagcg cgttgatcag gtgggtagtg agccagttca
      961 cgaacccatc agcgttcggg ggcttgacca aaggaacatc gtgattcagc ccgatgatcg
     1021 ttccgtcgtc tgcgactccg atcagtagag ttcctccgtc ggcgttgagg aagcccgcga
     1081 tgctcttcac gaccgcgtcc tccatccgct tgtccttgca gccgtcgcgg aggttccacc
     1141 tcgcggttga cttgaactcg gcctcaaatg tctcgtcgcc gcggatcagg tcgtctgtct
     1201 cgaccgcaaa gaatgcctcg tcaccgcgca gctgctccgc gaccagcgcg gcgaacgccc
     1261 ggtctgtcct gatcttctcc agcagatctg acttcaccgt ctcaacgtcg atctctgctc
     1321 caggcagatc gggtgatgcc atcccgggac tcagctgccc ggccggcggc atgtcccccg
     1381 cgtagacgct gctgccgtcg tcgtagtagc tcgcgaaaat gtgatcgaaa accaatcccg
     1441 tcttggcttc ccaaagctcc ggctcataga catcggggag ttcgtcgagg atctccttga
     1501 ccatcccccg ggcggcctcc cgggtttccg ccttcatccg ccagtcaaga accagcttct
     1561 cgtggatgtg atccagcagc tttttggaaa cggccttgac tgctttctcc tggtcttcag
     1621 tgagggccgg gtcgggcttg gtgagaaggt caaagatggc cagttctggt tcggtcaggc
     1681 cttccgtaac ggtgcgacgc tcctcttccg aaaggtcccg tgaaagaacc tgaagccgcc
     1741 gcagcatttc gtccacgttg aggcttcccg agttgtactg gtcaatcagg gttcgcattc
     1801 gctcgaccag gcttcttcgg gtcggattcc tttgcgccgc cgcctcgatt tccccgctca
     1861 acctgccggt cagtttctgg accgcagttc gcttctttcc cgccagccgt gcggcgagcg
     1921 catcgaagtc aatctggttg agatcgagca gcgcgtcggc gtcagcaccg tcggcagcgg
     1981 cacgaatcac atactcctcg gcgccgaccg agcggtctag aagtctctgg acctccccgg
     2041 cgacctggga gacatcgggc tgagggtcca gtgaacggat cgtttcggcg aggttccgaa
     2101 ctactccaac gatcccg
//
LOCUS       DAOQFI010000346         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30896_length_2114_cov_2.930063, whole genome shotgun sequence.
ACCESSION   DAOQFI010000346 DAOQFI010000000
VERSION     DAOQFI010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30896_length_2114_cov_2.930063"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1079)
                     /locus_tag="PLE93_06600"
     CDS             complement(162..1079)
                     /locus_tag="PLE93_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNH86751.1"
                     /translation="MSPGHPSGRQFEITHGSQSATVVEVGGGIRTYEVEGRSVLDPYP
                     VERICDGAHGAPLIPWPNRLDRGRYSFDGEAYQLPLTEPEQDNAIHGFLRWVTWAAEV
                     HGPDAVTMSTTVAPRPGYPFWLDVSVGYRLGDKGLEVSTEIVNKGERACPVGHGQHPY
                     LSAGSGLIDDCLLQHPGTVRILTDDRQLPAGREEASGTKFDFAEPRQLGDIEIDFAFT
                     DLRRDGEGKAWTRLEGADGKTASIWVDRNYPLVELYTGHTLAPDRARTGLGTEPMTCA
                     PDAFNNGEGLIRLEPGESASAVWGACLEG"
     gene            complement(1147..>2114)
                     /locus_tag="PLE93_06605"
     CDS             complement(1147..>2114)
                     /locus_tag="PLE93_06605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNH86752.1"
                     /translation="AGMAKIGFTLSSEEQPPGLLVEHAAAAEQAGFDFLSISDHFHPW
                     VDRQGQSPFVWSVLGAVSRATERIELITGVTCPTTRIHPAIIAQAAATTACLMPGRFS
                     LGLGSGENLNEHILGDRWPSVDIRQERLEEAVMVIRSLWEGGLTSHRGVHYEVENARI
                     YTLPDQLPPILIATAGEQATELAGRVGDGLVGLVPDSSVIEQFRESGGEGKPAYAQVH
                     ISWRESKEEAVKTALEWWPNAGVPGNLFVELPLPSHFSEAASTVDEDLIAESVVCGPD
                     PEPIVEEISRYLEAGFSHVYVHQVGPDTPGFFDFFRDRVLPEFQA"
BASE COUNT          357 a          697 c          706 g          354 t
ORIGIN      
        1 ccgccactct tcctggctcc actcgtccac gctgctgccg accaggagca ggtcgccagt
       61 ctggccggca tcgacgagcg aggcgagccc tggcaaaagc agccgggaag tcaggtcacc
      121 gctggcgcca aggatcagga atgtctgccg gtgcgccatg ttcagccctc gaggcaagcg
      181 ccccagacgg ctgacgcgct ctccccgggc tcgagccgga tcaacccctc gccattgttg
      241 aaggcatccg gagcacaagt catcggctcg gtcccgaggc cggtacgggc ccggtccggg
      301 gcgagcgtgt ggcccgtgta gagctccacc agcgggtagt tccggtcgac ccagatcgac
      361 gcagtcttcc cgtcggcacc ctcgagccgg gtccaggcct tcccctcccc gtcgcgacgc
      421 aggtcggtga acgcgaagtc gatctcgatg tccccgagct gacgcggctc ggcgaagtcg
      481 aatttcgtgc ccgacgcctc ctccctcccg gccggcaact ggcggtcatc ggtgaggatc
      541 cggacggtgc cgggatgctg gagcaggcaa tcgtcgatca agccgctgcc ggccgacagg
      601 tagggatgct ggccgtggcc gaccgggcag gcgcgctcgc ccttgttgac gatttcggtt
      661 gacacttcga gtcccttgtc gccaagccgg tagccgaccg aaacatcgag ccagaacgga
      721 taacccggcc ggggcgcgac cgtggtggac atcgtcactg cgtcgggtcc gtgaacctcc
      781 gccgcccagg tgacccagcg caggaagccg tggatcgcgt tgtcctgttc cggttcggtc
      841 aaaggcagtt gatatgcctc gccgtcgaag ctgtagcggc cgcggtcgag ccggttgggc
      901 cacgggatca ggggagcccc gtgggccccg tcgcagattc gttcgaccgg ataggggtcg
      961 agcacggatc gtccctcgac ctcgtaggtc cggatgccac cgccgacttc gacgaccgtc
     1021 gctgactgcg acccatgcgt gatttcgaac tgtcgtccgg aagggtgacc ggggctcatg
     1081 tgccaaaagt acccgaccgc ggcttccgcg acgggccgtt gcggcggggt gaggtccgga
     1141 ccggactcag gcctggaact ccggcagtac ccggtcgcga aagaagtcga agaaacccgg
     1201 agtgtcgggg ccgacctggt ggacatacac atggctgaag ccggcctcaa gataccggct
     1261 tatctcctcg acgatcggtt ccggatccgg gccgcagacg acagattcgg caatcaggtc
     1321 ctcgtccacg gtcgatgcgg cttcggagaa gtgcgaaggc agcggcagct cgacgaacag
     1381 gttgccgggc acgccggcgt tcggccacca ctcaagggcc gtcttcaccg cttcctcctt
     1441 cgactcgcgc caggaaatgt ggacctgggc ataggccggc ttgccttccc cgccggactc
     1501 gcggaactgc tcgatcaccg atgaatcggg aaccaggccg accagtccgt cccccacccg
     1561 tccggccagt tccgtcgcct gttcgccggc ggttgcgatc aggatcggag gaagctggtc
     1621 gggcagcgtg tagatccggg cgttctcgac ctcgtagtgg actccccggt gactggtcag
     1681 accgccctcc cacagtgagc ggatcaccat taccgcttct tcgaggcgtt cctggcggat
     1741 gtcgaccgat ggccagcggt caccaaggat gtgctcgttc aggttctcgc cgctgccgag
     1801 accgagggag aaacgtccgg gcataagaca ggcggtggtc gctgccgcct gggcgatgat
     1861 cgccgggtgg atccgggtag tcgggcaggt caccccggta atcaactcga tccgctcggt
     1921 cgcccgggat accgctccga gcaccgacca gacgaacggc gactgcccct gacggtcgac
     1981 ccaggggtga aagtggtcgg agatggagag gaagtcgaat cccgcctgtt cggcagcggc
     2041 tgcgtgctcc acgagcaggc cgggaggctg ttcttcgctg gaaagggtga aaccgatctt
     2101 tgccataccg gctt
//
LOCUS       DAOQFI010000347         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_30956_length_2112_cov_2.918814, whole genome shotgun sequence.
ACCESSION   DAOQFI010000347 DAOQFI010000000
VERSION     DAOQFI010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30956_length_2112_cov_2.918814"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLE93_06610"
     CDS             <1..409
                     /locus_tag="PLE93_06610"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86753.1"
                     /translation="VDEDVGDGRMKVRIDFSSPREFGFGDDYLDDPVVGTIICARAGD
                     SRVSHTSMAHVFLREGDGLRLRSHFWIGSRIRPRLPGPLAIAERPLESLASQSLVRGI
                     AIPGAVGPGLARHCAEEYANLNQILPGLFERFA"
     gene            complement(406..891)
                     /locus_tag="PLE93_06615"
     CDS             complement(406..891)
                     /locus_tag="PLE93_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH86754.1"
                     /translation="METHVFEIYIKTTPERLWQAITDPEERAKYSFGVKVHSDWTPGS
                     SYESKASWGPFVEEEGPIDISVGENLEVDPPHRLVQTFNAMWADEVKAEPTSRVTWEI
                     SQIGTSCRLKVTHDQLRDGANNQLYGGWPMILSGLKTLLETGELLDTPGSLQYVDGVE
                     S"
     gene            complement(896..1204)
                     /locus_tag="PLE93_06620"
     CDS             complement(896..1204)
                     /locus_tag="PLE93_06620"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH86755.1"
                     /translation="MDEVFKALADPTRRGLLDSLFERDGQTQKELEEGLEMTRFGVMK
                     HLKVLEEAGLVVTRRRGREKLHFLNPVPIRQVHDRWVSKFSEPWAAGLGELKRNLEKE
                     "
     gene            complement(1272..>2112)
                     /locus_tag="PLE93_06625"
     CDS             complement(1272..>2112)
                     /locus_tag="PLE93_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86756.1"
                     /translation="LVHEFLSTLVSGRRKLMNESVREWGTDSFMSFLRPLTRVESWSV
                     DGTPTKASCGNGTSSHEAPDADCECGLYAYHPWSGMGQHLVEDSGHRDGAVVGLVEAW
                     GRVEVHSEGFRAEYAKPVAFFLATSDSWDDHFTQGYIRVLRKLASGCGAEIIDTRRRG
                     AIGAWLENHPGQLDPDAVRRLLPGVQVGPVRIRPRHHALDLIGTGIERLIKGIGILLY
                     GIFMTVSISVQLIWWGFLLYVIFAAVTGLDPLGIGSHLGPDISDPTHPQQDSPTMTPG
                     QLR"
BASE COUNT          362 a          684 c          665 g          401 t
ORIGIN      
        1 ggtcgacgaa gacgtcggtg acggccggat gaaggtgagg atcgacttct cttcaccacg
       61 cgaattcggt ttcggtgacg actacctcga tgacccggtg gtcggcacga tcatctgcgc
      121 ccgggccggg gactcgcggg tctcccacac atcgatggcc catgtgttcc tgcgcgaagg
      181 cgacggcctg cggctgagga gccacttctg gatcggcagc cggatccggc cgcggctgcc
      241 gggcccgctc gccatcgcgg aacggccgct cgagtcgctg gccagccaga gcctcgtccg
      301 gggaatcgcg atccccggcg cggtcgggcc ggggctcgcc cgccactgcg ccgaggaata
      361 cgcgaacctc aaccagatcc tgcccggcct cttcgagcga ttcgcctagc tctcgacgcc
      421 gtcgacgtac tggagcgaac cgggcgtgtc gagcagctcg cccgtttcga gcagggtctt
      481 caggccggag aggatcatcg gccagccgcc gtagagctgg ttgttggcgc cgtcgcggag
      541 ctggtcgtgg gtcaccttga ggcggcagga ggtgccgatc tggctgatct cccaggtgac
      601 ccgggaggtc ggttctgcct ttacctcgtc ggcccacatc gcgttgaagg tctggaccag
      661 gcggtgcggc gggtcgacct cgaggttctc gccgaccgag atgtcgatcg gtccctcttc
      721 ttcgacgaac ggaccccagc ttgccttcga ttcgtaggac gaccctggcg tccagtccga
      781 gtgaaccttc accccgaacg agtatttggc ccgttcctca gggtcggtga tcgcctgcca
      841 gaggcgctcg ggtgtggtct tgatgtagat ctcgaatacg tgcgtttcca tcgtcctact
      901 ccttttcgag gtttcgtttg agctcgccca ggccggcggc ccagggctcg gagaacttgc
      961 tgacccagcg gtcatggacc tgccggatcg ggacggggtt gaggaagtgg agcttttccc
     1021 ggccgcgcct cctggtcacg accagccctg cctcttccag aaccttgagg tgcttcatca
     1081 cgccgaagcg ggtcatctcc agaccttcct ccagctcctt ctgggtctgc ccgtcccgct
     1141 cgaagagcga gtcgagcaat cccctccggg tcggatcagc caaagccttg aacacctcgt
     1201 ccatgcccgg gataataggt gactaattag tcacatgtca agtgtcccgc acggcggcca
     1261 ccgattacca gctatcgcag ctgcccgggc gtcatcgtcg gagaatcctg ctgtgggtgg
     1321 gtcgggtcgc tgatgtcagg cccgaggtgc gagccgatcc ccagcggatc gagtcccgtc
     1381 acggcggcga agatcacata caggaggaag ccccaccaga tcagctggac ggagatcgag
     1441 accgtcatga agattccgta gagcaggatg ccgattccct tgatcagtcg ctcgataccg
     1501 gtgccgatca ggtccaacgc gtggtgtctc ggacgaatac gaaccggccc gacttgaacg
     1561 ccgggcaaca gtcgccgaac agcgtcggga tcgagctggc ctggatgatt ctcgagccag
     1621 gccccgatcg cgcctcttcg gcgggtatcg atgatctcgg ctccacaacc agacgcaagc
     1681 tttcggagta cccggatgta gccctgcgtg aagtgatcgt cccagctgtc cgaagtggcc
     1741 agaaagaagg cgaccggttt ggcgtactcg gcccgaaatc cctcggagtg gacctcaacc
     1801 cggccccacg cttcgaccag tccgacgacg gcaccgtcgc ggtggccgga atcctcgaca
     1861 agatgctggc ccatgccgct ccaggggtgg tacgcataga gcccgcactc gcagtcggcg
     1921 tcaggcgctt catggcttga ggtcccgttt ccgcagctgg ctttggttgg cgtcccgtcc
     1981 accgaccagc tttcaaccct ggtcagcggg cgaaggaaac tcatgaacga gtcggtgccc
     2041 cactctcgca ccgactcgtt catgagtttc cttcgcccgc tgaccagggt tgaaagaaac
     2101 tcatgaacga gt
//
LOCUS       DAOQFI010000348         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31155_length_2105_cov_3.104878, whole genome shotgun sequence.
ACCESSION   DAOQFI010000348 DAOQFI010000000
VERSION     DAOQFI010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31155_length_2105_cov_3.104878"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLE93_06630"
     CDS             <1..894
                     /locus_tag="PLE93_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86757.1"
                     /translation="GVAAGIGFFIQQRREGRIGNPLYDLKIAGRRTFWVAGTAGIIVF
                     GTLMAAMFIGQQYLQNVLEYSTLGAGAAILPAALGMIVAAPRSARLVESHGSRFTLLC
                     GFVFCLIGFIFMLTLWDEGAAYWQVLAGYLPVGIGVGLAGTPSSNSLTSSVPVKRAGM
                     ASGTADLQRDLGGALMQSILGSVLTSAYASSFASQIAGNPEASQKVSESTQTQLERSF
                     GSAVTVAEQHPQYAPQIVHAAQTAFLNGSDSAYVVGIAAIVLGAVLVFFAFPKKEQEH
                     ALLAGFHAEDTGEPVPATATA"
     gene            908..2098
                     /locus_tag="PLE93_06635"
     CDS             908..2098
                     /locus_tag="PLE93_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HNH86758.1"
                     /translation="MKRTFVIGVGMTRFDKPGTKEGDYPDWAVEAGRKALEDAGITYD
                     EVQCAYVGYCYGESTSGERAVYGLGLSGIPIINVNNNCSTGSSALYLARQSVKGGLAD
                     CALALGFEKMEKGSLGAKYTDRTQPLDRHFIELTQLRQWEDAPPAPQIFGAAGREHME
                     KYGSRPEHFAWIGYKNHKHSVNNPYAQFQDEYTLQQIQDAPEIYSPLTKLQCSPTSDG
                     SGAAIVASEKFVDEHDLWEKAIEITGQALVTDTRETFDDDSAISLVGYEMSRLAAEQA
                     LNEAGTGIDEVDVVELHDCFSANELITYEALGLAERGHGHELVEAEATTYGGSTVVNP
                     SGGLISKGHPLGATGLAQCSELTWQLRGDADARQVEGAGKALQHNIGLGGAAVVTVYE
                     KPAA"
BASE COUNT          369 a          723 c          671 g          342 t
ORIGIN      
        1 ggagtggcgg ccggcatcgg cttcttcatc cagcagcgcc gggagggtcg catcggcaac
       61 cccctgtacg acttgaagat cgccggtcgc cgcaccttct gggtcgccgg cacggccggg
      121 atcatcgtct tcgggaccct gatggcggcc atgttcatcg ggcagcagta cctgcagaac
      181 gtgctcgaat actcgaccct cggggctggt gcggcgatcc tgcccgccgc cctgggcatg
      241 atcgtggcgg cgccccgctc ggccaggctg gtcgagtccc atggttcgcg cttcaccctg
      301 ctctgcggct tcgtgttctg cctgatcggt ttcatcttca tgctcacgct ctgggatgag
      361 ggtgcggctt actggcaggt cctggccggc taccttccgg tggggatcgg ggtcggcctg
      421 gccggcactc cctcctccaa ctcgctgacc agctcggttc cggtcaaacg cgccgggatg
      481 gcctccggga ccgcggacct gcagcgggac ctgggcggcg cattgatgca gtcgatcctc
      541 gggtcggtcc tgacttccgc ctacgcgagc tcgttcgcgt cccagatcgc cggcaatccg
      601 gaagcgagcc agaaggtcag cgaatcgacc cagacccagc tcgaacggtc cttcggcagc
      661 gcggtcacgg ttgccgagca acatccccaa tacgcgcccc agatcgtgca cgcggcacag
      721 accgcgttcc tgaacggcag cgactccgcc tatgtggtgg ggatcgccgc gatcgtcctt
      781 ggtgcggtcc tcgtcttctt cgccttcccg aagaaggagc aggaacacgc gctgctggca
      841 ggattccatg ccgaggacac gggcgagccc gttcccgcca ccgcaaccgc ctgagggagt
      901 tggagagatg aaacgtactt ttgtgatcgg ggtcggaatg accaggttcg acaagcccgg
      961 aaccaaggaa ggcgattatc cggactgggc cgtggaagcc ggccggaagg cacttgagga
     1021 cgcgggcatc acctacgacg aagtccagtg cgcctatgtc ggctactgct acggggaatc
     1081 gacctccggc gaacgggcgg tctacgggct cggcctttcc gggattccga tcatcaacgt
     1141 gaacaacaac tgctcgaccg gttcctcggc cctttacctg gcccgccagt cggtcaaggg
     1201 cggcctcgcc gattgcgccc tggcgctcgg cttcgagaag atggagaagg gctcgcttgg
     1261 tgccaagtac accgaccgca cccagccgct tgacaggcat ttcatcgagt tgacccaact
     1321 ccgccagtgg gaagacgcac cgcccgcccc gcagatcttc ggcgcggccg gtcgcgaaca
     1381 catggagaag tacgggtcca ggccggagca tttcgcctgg atcggttaca agaaccacaa
     1441 gcactcggtc aacaacccct acgcccagtt ccaggacgag tacacgctcc agcagatcca
     1501 ggacgccccc gagatctact cgccactgac caagctgcag tgctccccca cctcggacgg
     1561 ctcgggtgcc gcgatcgtcg cctccgagaa gttcgtcgac gagcatgacc tgtgggagaa
     1621 ggcgatcgag atcaccggcc aggccctggt caccgacacc cgggagacct ttgacgacga
     1681 ttcggcgatc agcctggtcg ggtacgagat gagccggctt gccgccgagc aggccctgaa
     1741 cgaggccggc accgggatcg acgaggtcga cgtggtcgaa ctccacgact gcttcagcgc
     1801 caacgagctg atcacctacg aggcactcgg cctggccgag cgcggccacg gccacgaact
     1861 ggtcgaggcc gaagccacca cctacggggg cagcacggtc gtcaacccgt ccggcggcct
     1921 gatctcgaag ggccatccgc tcggcgcgac cggcctcgcc cagtgttcgg agctcacctg
     1981 gcagcttcgc ggcgacgccg acgcgcgcca agtcgagggc gccggcaagg ccctccagca
     2041 caacatcggc ctcggcggcg ccgccgtcgt caccgtttac gagaagcccg ccgcctgagc
     2101 cgaaa
//
LOCUS       DAOQFI010000349         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31193_length_2104_cov_3.498780, whole genome shotgun sequence.
ACCESSION   DAOQFI010000349 DAOQFI010000000
VERSION     DAOQFI010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31193_length_2104_cov_3.498780"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLE93_06640"
     CDS             complement(<1..219)
                     /locus_tag="PLE93_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNH86759.1"
                     /translation="MNGEEVTDEDLDLTPYVCDFEEGDKVVYPHHGAGVVLKKADAEL
                     MGETREYLTIKILHNDLTVKVPTAKAGEA"
     gene            complement(279..1391)
                     /gene="disA"
                     /locus_tag="PLE93_06645"
     CDS             complement(279..1391)
                     /gene="disA"
                     /locus_tag="PLE93_06645"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HNH86760.1"
                     /translation="MSPAVESDLAHLSERQDPRLLRAIDAVAPGTELREGIDNILHAR
                     TGGLIVVGETEDFAFMLSGGIKLDIDYTPAMLYQVAKMDGAILVNADGSKITWANVQM
                     MPDPTIHSSETGTRHRTAERISKQVDALVIAISERREVVSLYLEGVKYILQDIPAVLA
                     KSNQALATLTKYRTRLDELSSRLTRAEFVGGAVLYDALMVLQRSELVSRMATEVERYV
                     IELGTEGRLIEMQLEETMVGVASERLALMRDYAVEDTEENVQFMLEELAALPHQDVLD
                     FGRLAELLGYDRKVNTLDLATEPRGYRMLGHVPRLPRLVVQKIVMNFGSVEAALAASD
                     EELAAVDGVGPARAKEIREGVRRLQESVMADRFLNT"
     gene            complement(1515..>2104)
                     /locus_tag="PLE93_06650"
     CDS             complement(1515..>2104)
                     /locus_tag="PLE93_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86761.1"
                     /translation="GLGSPRRAPRPARFVEEATGAQGSVVLASMEGTRPVLVEVQALV
                     SPSELEIPRRVVNGIDRNRLSMILAVLARHARLGLGTADVFVNVVGGVRIDEPGADLA
                     VALAVASAQRGETLSGPDGRPLACFGELGLTGDLRYVAHADRRVAEAAKFGLEPVIGP
                     RGGERLSGLAPCATVKQALKAAFSGSSADSRAAAA"
BASE COUNT          350 a          689 c          662 g          403 t
ORIGIN      
        1 ggcttcgccg gccttcgcgg tcgggacctt gacggtcagg tcgttgtgca ggatcttgat
       61 cgtcaggtac tcgcgggtct cgcccatgag ttcggcatcg gccttcttca gaacaacgcc
      121 tgctccgtgg tgcggataga cgaccttgtc gccttcctcg aaatcgcaca cgtacggagt
      181 cagatcgaga tcttcatcgg tcacttcttc gccgttcact gcctctgaat cggaaatcag
      241 atgctccttt gtctcttagc tgacgactga atgcgtcgtc atgtgttcaa aaaccggtct
      301 gccatcacgg actcctgaag cctccgcact ccttcgcgga tctccttggc ccgggccggg
      361 ccaacgccgt cgacggcggc aagttcctcg tcgctggccg cgagcgcggc ttcgacgctg
      421 ccaaagttca tgacgatctt ctggaccacg agccgcggca ggcgcggaac gtggccgagc
      481 atcctgtagc cgcgcggttc ggtggccagg tcaagcgtgt tgaccttgcg gtcatagccg
      541 agcagttcgg ccaggcggcc gaagtcgagc acgtcctgat gcggcagggc ggccagttcc
      601 tcgagcatga actggacgtt ctcctcggtg tcctcaaccg cgtaatcgcg catcagtgcg
      661 agccgctccg aagcaacccc gaccatggtc tcctcgagct gcatttcgat cagcctgcct
      721 tcggtgccga gctcgatcac gtatcgctcg acctcggtcg ccatccggga aaccagctcc
      781 gagcgctgca gcaccatcag tgcgtcgtag aggacggcgc cgccgacgaa ttcggcccgg
      841 gtcaggcggc tcgagagctc gtcgagccgg gtccggtact tggtcagcgt ggcgagggcc
      901 tgattggact tggccagcac cgcgggaatg tcctgaagga tgtacttgac cccttcgaga
      961 tagagcgaga ccacttcccg ccgttccgag atcgcgatga ccagggcatc gacctgcttc
     1021 gagatccgtt cggcggtgcg atggcgggtg ccggtctccg aggagtggat ggtcgggtcg
     1081 ggcatcatct ggacgttggc ccaggtgatc ttgctgccgt cggcgttgac caggatcgcc
     1141 ccgtccatct tcgccacctg gtagagcatc gccggcgtgt agtcgatgtc cagcttgatg
     1201 ccaccggaaa gcatgaaggc gaagtcctcg gtttccccga ccacgatcag accgccggta
     1261 cgggcgtgga ggatgttgtc gatcccttcc ctcagctcgg tgccgggggc gacggcgtca
     1321 atcgcgcgca gcagtctggg atcctgacgt tcagacagat gtgccagatc actctctacc
     1381 gctggtgaca ttcagagaat tatgccagat tcggctttct aatgcggtct ggcgtggttc
     1441 cgggcgctct tttgcggggg ttgtgaccgg ccctcaatcc gctccggaaa gccgaattta
     1501 gcctcgcctg aagatcaggc tgcggcggcc cgggaatcgg ctgaacttcc ggaaaatgcg
     1561 gctttcaggg cctgcttcac ggtggcgcag ggagcgagcc ccgatagccg ctctccaccc
     1621 cttgggccga tcaccggttc gaggccgaat ttcgctgcct cggcgactct gcggtcggca
     1681 tgggcgacgt acctcaggtc cccggtcagg cccagttcac cgaaacaggc gagcggacgg
     1741 ccatcaggcc cggaaagggt ctcgccgcgc tgggccgaag cgaccgcgag cgccacggcg
     1801 agatcggccc cgggctcatc gatcctcacc ccgccgacca cgttcacaaa gacgtcggcc
     1861 gtaccgagac ccagcctcgc gtgccgggcc agcaccgcaa ggatcatgct gaggcggttg
     1921 cggtcaatgc cgttgacgac ccttcgcgga atctcgagtt cactcgggga gaccagagcc
     1981 tgcacctcga ccagcaccgg tctggtccct tccatcgagg ccagaaccac cgacccctgg
     2041 gccccggtcg cttcctcgac gaagcgggcc ggccgcggag ctcgtcgcgg ggagcccagc
     2101 cctt
//
LOCUS       DAOQFI010000350         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31330_length_2099_cov_3.534736, whole genome shotgun sequence.
ACCESSION   DAOQFI010000350 DAOQFI010000000
VERSION     DAOQFI010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31330_length_2099_cov_3.534736"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLE93_06655"
     CDS             <1..734
                     /locus_tag="PLE93_06655"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HNH86762.1"
                     /translation="ERPLAGRTALVTGASRGIGEAIATVLARDGATVIGLDIPPAAAE
                     LDQVTSRLGGRSLTLDITTPDAPDAIARELGEGVDLIVHNAGVTLDRTIAKMPEERWS
                     KLMEINLSSEERINDAVLSADLLNANGRIVCVSSMSGIAGNSGQTNYAASKAGVIGMV
                     ESMAPELARRQATINAVAPGFIETQMTAAMPFGTREVGRRLNSLSQGGKPVDVAETIA
                     WFASPASTGVNGNVVRVCGQNLLGA"
     gene            734..1642
                     /locus_tag="PLE93_06660"
     CDS             734..1642
                     /locus_tag="PLE93_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNH86763.1"
                     /translation="MTRLESSPSILPLYLKAAMPLIPGASSLPFLPGGGGEIPSGLGF
                     ELKGVVPQQDQVAEYAKVCGFPLKDRLPPTFPHVLAFPLHMAVMSNGEFPFGAVGLVH
                     IENSINQLRPIAFGEMLEVKVEVTSLSPHPKGQIFSLLTEVSAAGETVWTETSTMLRR
                     GKGSQTPSAPGLEIPTLDPGTPASATWKLPADLGRRYGAVSGDRNPIHMHALTAKPLG
                     FHSAIAHGMWTAARALAQIESRLPDSFTAGVRFRKPIFLPARVQFAAEGDGTETLFAV
                     RDARKGTPHMDGRITSNANETAKASN"
     gene            1644..>2099
                     /locus_tag="PLE93_06665"
     CDS             1644..>2099
                     /locus_tag="PLE93_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH86764.1"
                     /translation="MSSKNSQGLADKTMGSSLRALNWIAGSKILDRLRLRSQFERTVF
                     QSTKHGMRAATSAGRSFNKATSLGKPARQKSGSGSGLFDLTPDDEQQMFIEAGQAFAA
                     DQIRPAASGADSEKATPAAILEQAGEIGVNTLGIPEEFDGVLTEQSTVTW"
BASE COUNT          387 a          731 c          657 g          324 t
ORIGIN      
        1 gggaacggcc cctggcggga aggactgcgc tggtcaccgg ggcttcacgg ggaatcggag
       61 aggcgatcgc caccgtcctc gcgcgggacg gggcgaccgt catcggactc gacattccgc
      121 ccgccgcggc cgagctggac caggtgactt cgcgcctcgg cgggcgttcc ctgaccctgg
      181 acatcacgac ccccgatgcg cctgacgcga ttgcccgcga gctcggcgaa ggagtcgacc
      241 tgatcgtcca caacgccggc gtcactctgg accgcacgat cgccaagatg ccggaagagc
      301 gctggagcaa gctgatggaa atcaacctct ccagcgagga acggatcaat gacgcggtcc
      361 tttccgccga cctgctgaac gccaacggcc ggatcgtctg cgtttcatcg atgtccggca
      421 tcgccggcaa ttcaggccag accaattacg ccgcttccaa ggccggtgtg atcggcatgg
      481 tcgaatcgat ggcgcccgag ctggcgcggc gacaggccac gatcaacgcg gtcgcccccg
      541 gattcatcga gacgcaaatg accgcggcga tgccattcgg cacccgtgag gtcggccgcc
      601 gcctgaacag cctctcgcaa ggtggcaagc cggttgacgt ggcggagacg atcgcctggt
      661 tcgccagccc cgcctcaacc ggggtcaacg gaaacgtggt ccgggtctgc ggccagaacc
      721 tgctgggagc ctgatgacca ggctcgaatc ctccccgtcg attctgccgc tctatctgaa
      781 ggcggcgatg ccgctgatcc ccggcgcctc ctccctgccc ttcctgcctg gcggcggcgg
      841 ggagattcct tccgggctcg gcttcgaact gaagggagtg gttccgcaac aggaccaggt
      901 agccgaatac gccaaggtct gcgggttccc gttgaaggac aggctgccgc caaccttccc
      961 ccacgtgctg gcctttccgc ttcacatggc ggtgatgagc aacggggagt ttcccttcgg
     1021 cgcggtcggc ctggttcaca tcgaaaactc gatcaatcaa ctcaggccga tcgcattcgg
     1081 cgagatgctt gaagtgaaag tcgaagtcac atccctctcc ccccacccga aggggcagat
     1141 cttttccctg ttgaccgagg tcagtgccgc cggggagacg gtctggaccg agacgagcac
     1201 catgctgcgg cgcgggaagg gcagccagac gccctccgct ccgggcctcg aaatccccac
     1261 gcttgacccc gggactccgg ccagcgccac ctggaaactc ccggccgacc tcggccggcg
     1321 ctacggcgcg gtctccgggg atcgcaaccc gatccacatg catgccctga ccgcaaagcc
     1381 actgggcttc cactccgcga tcgctcacgg gatgtggacc gccgcccggg ccctggccca
     1441 gatcgagagc cggctgcccg attccttcac tgccggggtc aggttccgca agccgatctt
     1501 cctgcccgcc cgggttcagt tcgccgccga aggcgacggc acggaaaccc tctttgccgt
     1561 ccgcgatgcc cgcaagggca cccctcacat ggacggtcgc atcacttcca acgcgaacga
     1621 aaccgcgaaa gcgagcaatt gaaatgagca gcaagaacag ccaggggctg gccgacaaga
     1681 cgatgggctc cagccttcgt gccctgaact ggatcgccgg atcgaagatc ctcgaccggc
     1741 tcaggctgcg cagccagttc gagcggaccg tctttcagag cactaaacac ggaatgcgcg
     1801 ccgcgaccag cgccggccgc agcttcaaca aggcgaccag cctcggcaag ccggcccgcc
     1861 agaagagcgg ctccggatcc gggctcttcg atctcactcc ggatgacgaa cagcagatgt
     1921 tcattgaggc cgggcaggcc tttgcggccg accagatccg gcccgccgcc tccggggcgg
     1981 actcggagaa ggccactccg gccgcgatcc ttgagcaggc cggcgagatc ggcgtcaaca
     2041 cacttggcat ccccgaggaa tttgacgggg tcctgaccga gcagtcgacc gtaacctgg
//
LOCUS       DAOQFI010000351         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31434_length_2096_cov_2.585497, whole genome shotgun sequence.
ACCESSION   DAOQFI010000351 DAOQFI010000000
VERSION     DAOQFI010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31434_length_2096_cov_2.585497"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLE93_06670"
     CDS             <1..206
                     /locus_tag="PLE93_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNH86765.1"
                     /translation="VEAGGISEVILATNPTTTGEATASHIADLLRTQTKVTRLAAGLP
                     VGADLEHADEVTLGRAMAGRGSI"
     gene            complement(215..1264)
                     /locus_tag="PLE93_06675"
     CDS             complement(215..1264)
                     /locus_tag="PLE93_06675"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH86766.1"
                     /translation="MSEHIWYTHAPSPTAFGIALRKGWIHEEMARCGVEVRALAASSD
                     PDVQRLRRERTSPRAFRQGGNVAPLIARSRGNDVRLIGLSRTSSIHPVLALPESGYRT
                     AADLLGRRIAIPVRKNAVVDIPRATALRTVSNLLASAGLTLNDVELVAIPVEGSYFEA
                     RAKPGEPIADAPREGVAFQVSSAHSNELLALIRGEVDAIASEGSTAAIIASTFGLTTL
                     EPGRAVNRDLNNRSLLAVTVSGGLLDRGPGLVTSVLVQILAAAEWSIDEEAEARRLIA
                     LDAGIHEDLLDAAYSPRVSRQLGVDLRMDKVADLREQHDFLLDRGIIERPVDLDEFIE
                     PEPLKTALARRGVLV"
     gene            complement(1346..>2096)
                     /locus_tag="PLE93_06680"
     CDS             complement(1346..>2096)
                     /locus_tag="PLE93_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNH86767.1"
                     /translation="NLSDRWWVGRGPNGGYVAALMLRAMTGEVASMEPEAALPPRSLS
                     VHFLLRPEAGPAEIEVTVEHAGRVTAFLSARMVQSGEVKAKAMAVFSGEREGPAFDNS
                     LMPKVPPPEELEEWDTEQAPVAVFGRYRAMMIKGIPGEAGERAETMGWIRLRDEQPVD
                     PVLAAAVLDVWYPAPFVVFEKIPFAPTLEYTVHFPRTIPEPGPPDWNLIRLTAEEGTE
                     GHFTEDAELWSRDGKLLAKARQMALLRHPLG"
BASE COUNT          365 a          675 c          695 g          361 t
ORIGIN      
        1 gggtcgaggc gggagggatc agcgaggtga tcctcgccac caacccgacc acaaccggag
       61 aggcgaccgc ctcacacatc gcggacctgc tccgaaccca gacgaaggtg acccgcctgg
      121 ccgccgggct gccggtcggc gcagacctcg aacatgccga tgaggtgaca cttggccggg
      181 cgatggctgg ccgagggtcg atctagatcg ggcctcagac caggaccccc cggcgggcaa
      241 gggcagtctt caggggttcc ggttcgatga attcatcgag gtccacgggt cgctcgatga
      301 ttccgcggtc gagcaggaag tcgtgctgtt cccggagatc agcgactttg tccattctca
      361 ggtccacgcc gagttgccgg ctgacccgag ggctgtaggc cgcgtccagg aggtcctcgt
      421 ggatgcccgc gtcgagggcg atcaggcgcc tcgcttcagc ctcttcgtct attgaccact
      481 cggcggcggc aaggatctgg accagaacgc tggtgaccag acccggcccg cggtcgagca
      541 gcccgccact taccgttacc gccagcagcg agcggttgtt gaggtcgcgg ttgactgctc
      601 tgccgggctc gagggtggtc aggccgaacg tcgaggcgat gatcgccgcg gtcgagccct
      661 cggatgcgat cgcatcgacc tcacccctga tcagcgcgag cagctcattg ctgtgcgcgg
      721 aggaaacctg aaaggcgacg ccttccctgg gggcatcggc gatcggctca cccggcttcg
      781 ccctcgcctc gaagtaggag ccctcgaccg ggattgccac gagctccacg tcgttcaacg
      841 tgaggccggc cgaggcgagc aggttgctga ctgtccggag ggctgtggcc ctcgggatgt
      901 cgaccacggc gttcttcctc acaggaatcg cgatgcggcg gccgagaagg tccgctgctg
      961 tccggtagcc ggactccggg agggcgagca ccgggtgaat gctcgaggtg cgcgaaaggc
     1021 cgatcagcct cacgtcgttt cctcgggagc gggcgatgag cggagctacg tttccgccct
     1081 ggcgaaaggc cctgggggag gtgcgctcgc gcctcaggcg ctggacatcc gggtcagacg
     1141 aggccgccag ggccctgacc tcgactccgc accgggccat ctcctcatgg atccagccct
     1201 tcctcagggc aatcccgaaa gcagtgggtg acggggcgtg tgtgtaccag atgtgctcgc
     1261 tcattcgggg cattgtagtc aaacttgacc aagaaagtca taaatgttgg ctaaaggatg
     1321 aagcggcttg gtcggaccct ggggatcagc ccaatgggtg ccgtaggaga gccatttggc
     1381 gcgccttggc gagcagcttg ccatcccggc tccagagctc ggcatcctcg gtgaagtggc
     1441 cttcggttcc ctcttcggcg gtcaaccgga tcaggttcca gtccggcgga ccgggctcgg
     1501 ggatggttcg cgggaagtgc accgtgtact ccagggtcgg agcaaaggga atcttctcga
     1561 aaacgacgaa cggcgccgga taccagacgt cgagcaccgc ggccgcgagg acggggtcga
     1621 ccggctgctc atcacgcagc cggatccagc ccatggtttc tgccctctca ccggcttctc
     1681 cgggaatgcc cttgatcatc atcgcgcggt aacggccgaa aaccgcgacc ggggcctgct
     1741 cggtgtccca ttcctcgagt tcttccgggg gcggaacctt cggcatcagg gaattgtcaa
     1801 aggccggccc ttcacgctca cccgagaaaa ctgccatcgc cttcgctttc acttcaccgc
     1861 tctggaccat gcgggcagaa agaaacgcgg tcactcttcc cgcatgctcg accgtgacct
     1921 cgatctcagc aggaccggct tcgggccgga gcaggaagtg gacgctgagc gacctgggag
     1981 gcagtgccgc ctccggttcc atcgacgcta cctcaccggt cattgcccgg agcatcaagg
     2041 ctgcgacata gccgccgttg ggtccgcgac caacccacca gcggtcactc aggttg
//
LOCUS       DAOQFI010000352         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31451_length_2095_cov_3.691667, whole genome shotgun sequence.
ACCESSION   DAOQFI010000352 DAOQFI010000000
VERSION     DAOQFI010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31451_length_2095_cov_3.691667"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PLE93_06685"
     CDS             complement(<1..843)
                     /locus_tag="PLE93_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86768.1"
                     /translation="MFQTLMTSTFRRRFGLMLLLLPVLFTLSQPDPGKANAGLTIPST
                     TTRAATAPFNKAAMWIWYIDDSNGGNIGSIIRQARRAHIGTLYIKSGDGVDAWSQFNR
                     PVVRRFHRAGLKVCGWQYIYGRRPIAEAKISAVAKRRGADCFVIDAESEFEGAYAAAD
                     WYIRSLRQRVGRRFPIGLAGFPYANYHPGYPYSVFLGPGAANANVPQVYWHTIGDSVR
                     TALEITWEQNSIYKRPIFPVGQTYEDPGARELLAYRRFMLNYGTRPSWWSWQETSPAE
                     WKILA"
     gene            1000..>2095
                     /locus_tag="PLE93_06690"
     CDS             1000..>2095
                     /locus_tag="PLE93_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86769.1"
                     /translation="MRRYLVLLAALLAGMFIVGGVADAAGKQVKPARPSVKILTPASS
                     QLAKGTVRIQVKANKGAKVRLSAIARSFDTPNQRLFTNRTLYLRKKAGMAISMSLTPE
                     AKATARTCQALTLIITAKNGKKTTRVTRSVNRDLGDCQLSPVDLSRAADCDFIAEPKE
                     GMCMLPFPDDYYTVEDESSPTGKRIHFTDAGMPVNAVNEPISAASYDLSDGFSQGQGI
                     VVKIPGVESQAAVDANDFVPINHIGQYADKNQKAVVINADTGERHPIWVNIDANAGSD
                     QDRVLEIKPAVNFDEKGHYIVALRNLVTAAGKPIAAPTAFRYYRDQIRSSQKPINDRR
                     AHFEQMFETLKQAGIKRRDLYLAWDFTTASN"
BASE COUNT          411 a          669 c          671 g          344 t
ORIGIN      
        1 ggcgaggatc ttccattcgg ccgggctggt ttcctgccag gaccaccagc tcggccgggt
       61 gccgtagttg agcatgaagc ggcggtaggc gagcagctcc cgggcaccgg gatcctcgta
      121 ggtttggcca accgggaaga tcggccgctt gtagatgctg ttctgctccc aggtgatctc
      181 cagcgcggtc cgcaccgaat ccccgatcgt gtgccagtag acctgcggca cattcgcatt
      241 tgccgccccc gggccgagaa agaccgaata ggggtagccc gggtggtagt tggcgtacgg
      301 gaagcctgcg agcccgatcg ggaaacggcg tccgacccgt tggcgaaggg aacggatgta
      361 ccagtcggcg gcagcgtagg cgccttcgaa ttcggactcg gcgtcgatca cgaagcagtc
      421 cgccccgcgc cgcttggcga cggccgagat cttcgcttcg gcgatcggcc gcctgccgta
      481 gatgtactgc cagccgcaga ccttgagccc ggcccggtga aaacgccgga cgacgggccg
      541 gttgaactgg ctccaggcgt cgacgccgtc accggactta atgtagagcg tgccgatgtg
      601 ggcccgccgg gcctggcgga tgatcgaacc gatgttgcca ccgttggaat cgtcgatgta
      661 ccagatccac atcgctgcct tgttgaaggg agcggtcgcc gccctcgtcg tcgtggacgg
      721 gatcgtgagg ccggcgttgg ccttgccggg atccggctgg gaaagcgtga acaggaccgg
      781 cagcagcagg agcatcagcc cgaagcgccg gcggaaagtc gatgtcatca gggtctggaa
      841 cacggttgat ggctgatcgg agcggattca gcctcgcagg agaacccccg gcaagcggcg
      901 gcctcggacc cgcaagcaag ctttctttca tttcacctcc ctgccctgta gcctgcgggg
      961 cagggagaag ttcaaactct gtttgggagg aatcaaagga tgcgtcgtta cctcgttctg
     1021 ctggcggctc tgcttgccgg aatgttcatt gtcggcggag ttgcagacgc tgccggcaag
     1081 caggtgaagc cagccaggcc ttcggtcaag atcctgaccc cggcgtcaag ccagctcgcc
     1141 aagggcaccg tcaggattca ggtcaaagcg aacaaggggg cgaaagtgcg cctctcggca
     1201 atcgccagat ccttcgacac gcccaaccag cgtctcttca cgaaccgcac gctctacctg
     1261 cgcaagaagg cgggcatggc gatcagcatg agcctgactc cggaggcaaa ggccaccgcg
     1321 cggacctgtc aggccctgac cctgatcatc actgcgaaga acgggaagaa gacgaccagg
     1381 gtcacgaggt cggtcaaccg ggatctcggc gactgtcagc tcagcccggt cgatctgagc
     1441 cgggctgccg actgcgactt catcgccgaa ccgaaggaag gcatgtgcat gctgcccttc
     1501 cccgacgatt actacacggt cgaggacgag tccagcccga ccggcaagcg aatccatttc
     1561 accgacgccg gcatgccggt taacgctgtg aacgagccga tctcggcggc cagctacgac
     1621 ctttcggacg ggttcagcca gggccagggc atcgtcgtga agatcccggg ggtcgagtcc
     1681 caggccgcgg ttgacgcgaa tgacttcgtg ccgatcaacc acatcggtca gtacgccgac
     1741 aagaaccaga aggcggtcgt gatcaacgcc gacaccggcg agcgccatcc gatctgggtc
     1801 aacatcgacg ccaacgccgg ttccgaccag gaccgggtgc tggagatcaa gcccgccgtc
     1861 aacttcgacg agaagggcca ctacatcgtc gccctgcgca acctcgtcac tgccgccggc
     1921 aagccgatcg cggcgccgac cgccttccgc tactaccggg atcagatccg ctccagccag
     1981 aagccgatca atgatcgccg ggctcatttc gagcagatgt tcgagacctt gaagcaggcc
     2041 ggaatcaagc gccgggacct ctaccttgcc tgggacttca ccaccgccag caacg
//
LOCUS       DAOQFI010000353         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31462_length_2095_cov_2.710294, whole genome shotgun sequence.
ACCESSION   DAOQFI010000353 DAOQFI010000000
VERSION     DAOQFI010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31462_length_2095_cov_2.710294"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLE93_06695"
     CDS             complement(<1..534)
                     /locus_tag="PLE93_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNH86770.1"
                     /translation="MQKFVIQGGVPLSGEITAAGNKNAALPILAACLLTEEVVTIANV
                     PRIRDTEAQLALLESIGVEVEWVEENTVRLCAREVENKALDEDLSAAIRASFLLAGPL
                     LARFGSVEMPPPGGDFIGRRRLDAHLDAFRDLGARVGGKRWIELQAPPSGLKAAKIFM
                     DEPSVMGTENALMAAALT"
     gene            625..1872
                     /locus_tag="PLE93_06700"
     CDS             625..1872
                     /locus_tag="PLE93_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH86771.1"
                     /translation="MAQASTGKRRGVGSRIGWPLKIFVGILLVLLVLLILNAFALNNE
                     TKSASVNVPGAQLVETTAGKLQVLDTGTPSGSMSAGQSLPIVLIHGSAGAINWWDDLI
                     PLLKPYHRVIAIDLLGYGGSDKPDSDYSIETQANLVAQALGKLNISRAVLVGHSLGGS
                     VVTSVAEQSPDLVSGLVLIDSGPDSSYGGLSGAAKASRTPILGQALWRLAPDSMIKKN
                     VSQGFAPDYDVPDKYVQDVRNMTYSAYRKSSTGVEDYTDGKSLPDRLAELGKPLLVIF
                     GEEDQIYDAREALSAYAAVPGAKTHLILNSGHSPQIEAPAQTARAIENFTRTITRSEL
                     AAPRKAAVAQAKKEAAESKKESGQKANRQPDQAQQNAGNKSEQAKQKAAQLKQQASEK
                     ARKARQEALQAQKEAAGSKVESP"
BASE COUNT          403 a          691 c          659 g          342 t
ORIGIN      
        1 ggtcagggcg gcggccatca gggcgttctc ggtgcccatc acggagggct cgtccatgaa
       61 gattttggcg gccttcaggc ccgaaggcgg cgcctggagc tcgatccagc gcttgccgcc
      121 gacccgggct ccgaggtcgc ggaaggcgtc aaggtgggca tcgaggcggc ggcggccgat
      181 gaagtcgccg ccgggcggcg gcatctcaac cgagccaaac cgggccagga gcggaccggc
      241 cagaaggaag gaagcgcgga ttgcggccga gaggtcttcg tccagggcct tgttctcgac
      301 ctccctggcg cagagccgga ccgtgttctc ctcgacccac tcgacctcga ccccgatcga
      361 ctcgagcagc gccagctggg cctcggtgtc ccggatccgg ggtacgttcg caatcgtcac
      421 gacctcctcg gtgaggaggc atgcggcgag gatcggcagc gcggcgttct tgttgcctgc
      481 ggccgttatc tcgccggaga gcggaacccc gccctgtatt acgaacttct gcatctgttt
      541 ttgtaccccg cggtttgacg gttttcgtgt gaccggacgg ggacattgca gcccgaccga
      601 agcgggtcta gggtaacacc ggtcatggca caagcttcca ctggcaagag gcggggcgtc
      661 ggctcccgga tcggctggcc cctcaagatc ttcgtgggga tcctcctcgt gctgctggtc
      721 ctgctcattt tgaacgcatt cgcgctcaac aacgagacca aatcagcgag cgtgaacgtg
      781 cccggagccc aactggtcga aaccacggcc ggcaagctcc aggtgctgga tacgggcaca
      841 ccatccgggt cgatgtccgc cggccagagc ctgccgatcg tcctgattca cggttcggcc
      901 ggggcgatca actggtggga tgacctgatc cccctgctca agccgtatca ccgggtcatc
      961 gcgatcgacc tgctcggtta cggcggctcg gacaaaccgg attccgacta ctcgatcgag
     1021 acccaggcga atctggtcgc ccaggccctg ggcaagctca acataagccg ggccgtgctg
     1081 gtcggccatt cgcttggtgg cagtgtggtc acctcggtcg ccgagcagtc ccccgacctt
     1141 gtttccggac tggtcctgat cgacagtggt cccgacagct cctacggcgg gctttccgga
     1201 gcggcgaagg cctcccgcac gccgatactg ggtcaggccc tgtggcgact cgcccccgac
     1261 tcgatgatca aaaagaacgt ttcacagggg ttcgcgcccg attacgacgt gcccgacaag
     1321 tacgtccagg acgtccgcaa catgacctac tccgcgtaca ggaaatcctc aaccggagtc
     1381 gaggattaca ccgacgggaa gtcgctcccc gaccggctgg ccgaactcgg caaaccgttg
     1441 ctggtcatct tcggcgagga agaccagatc tacgacgccc gggaagccct cagcgcctac
     1501 gccgccgtac cgggcgcaaa gacccacctg atcctgaact ccggccactc gccccagatc
     1561 gaggccccgg cccagaccgc cagggcaatc gagaacttca cccggaccat caccaggagc
     1621 gaattggccg cgccccggaa ggcggcggtt gctcaggcca aaaaggaagc cgccgagtcg
     1681 aagaaggaat ccggacagaa ggcaaaccgg caacccgacc aggcccaaca gaacgccggg
     1741 aacaagtctg aacaggcaaa gcagaaggcc gcgcagctca agcagcaggc cagcgagaag
     1801 gcccggaagg caaggcagga agccctgcag gcccagaagg aagcagccgg gtccaaggtc
     1861 gagtcaccct gaccgtccgg ctttcgccgg cgctcctccc cgtttccgcc tacgccggcc
     1921 tcgacggtcg tcaaccgctg ctttccggcc acgaagccgg aaatcatgcg ggaaactact
     1981 gattttcgct gcttttcagc cggtaaggtt cgcgccgttc aagccggatt tccctgcctg
     2041 agctaaagga gcgaacatgg aagtcacgtt cgggggtttt cagattgcag tgctt
//
LOCUS       DAOQFI010000354         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31589_length_2091_cov_2.857564, whole genome shotgun sequence.
ACCESSION   DAOQFI010000354 DAOQFI010000000
VERSION     DAOQFI010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31589_length_2091_cov_2.857564"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PLE93_06705"
     CDS             <1..1169
                     /locus_tag="PLE93_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNH86772.1"
                     /translation="WIASSRSDKYGRRALILTSGIIFIIGAIVAAISPGTWVLVASRV
                     VIGVAIGVASAVAPVYISEIAPPDIRGRLVTFFQLAVTIGILVAYLVGLAFADSNEGW
                     RWMLGLGAVPALALVIGIIRLPSSPRWLLMVGREDDAREALQRVRTEGPEAIELEIKE
                     IESSMSIHEGSWRDLLAPAVKAALVVGIGLAILQQITGINTVIYYAPTIIQETGIDSD
                     ASSILASLGVGIVNVLMTVVALRLLDTRGRREMLFVGVSGISISLFILGLAFLIGADS
                     TFATVMAIGSLMLFVSSFAISLGPIFWLLNAEIYPLSVRSKAASAGTMTNWFFNFLVS
                     LTFLPLIDLLSQTGAFWFYGAIGLVTLWFCWKYVPETKGRSLEQINDIFERRAR"
     gene            1291..1833
                     /locus_tag="PLE93_06710"
     CDS             1291..1833
                     /locus_tag="PLE93_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH86773.1"
                     /translation="MSQTPSCSIRDFRIEDAEAVHRWFNNKEATKTLMEQRESFGMEN
                     AEGWTRAAMKQEGEDRKYAIEVPGIEQPVGFTALYGLFRQTAPELGCLIGDEVRGKGV
                     GRWAERLTVTKAFEEFGAHRIYGRIPAFNEAAKKVVTAQGWTYEGNMPGHIRRPDGTM
                     LDCEIWGATPEQFFAAVGKW"
     gene            1853..>2091
                     /locus_tag="PLE93_06715"
     CDS             1853..>2091
                     /locus_tag="PLE93_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86774.1"
                     /translation="MRLAAELVADGGRAASGPDFFRSAEFLEAEGTTHSLLIFEDGDE
                     TELVASTPLIVREIPGSDRLDAISPYGYPGLAISDG"
BASE COUNT          333 a          713 c          667 g          378 t
ORIGIN      
        1 cctggatcgc cagctcccgc tctgacaagt acggccgccg cgccctgatc ctcacctcgg
       61 ggatcatctt catcatcggc gcgatcgtcg cggcgatttc acccggtacc tgggttctgg
      121 tcgcttcccg tgtggtgatc ggcgtcgcga tcggcgtcgc ctccgccgtg gcgccggtct
      181 acatcagcga gatcgccccg cctgacatcc ggggcaggct cgtcacgttc ttccagctgg
      241 cggtgacgat cggcatcctc gtcgcctacc tggtcggtct cgcctttgcc gattcgaacg
      301 aaggctggcg atggatgctc gggctcggcg ccgtgccggc gctggcgctc gtgatcggga
      361 tcatccggct gccctccagc ccgcgctggc tgctcatggt cggccgcgag gacgatgcgc
      421 gcgaggcgct gcagcgggtc aggaccgaag gccccgaagc gatcgagctg gagatcaagg
      481 agatcgagtc cagcatgagc atccacgagg gctcctggcg ggatctgctc gcccctgccg
      541 tgaaggcggc gctggtggtc ggcatcgggc tggcaatcct ccagcagatc accggcatca
      601 acacggtcat ctactacgcc ccgacgatca tccaggagac cgggatcgac tccgacgcct
      661 cctcgatcct cgcctcgctc ggggtcggca tcgtcaacgt gctgatgacc gtggtcgcgc
      721 tgcgattgct ggacacccgc ggccgccgcg agatgctctt cgtcggcgtg tcggggatct
      781 cgatttcgct gttcatcctc ggcctcgcct tcctgatcgg cgccgattcg accttcgcca
      841 cggtgatggc gatcggcagc ctgatgctct tcgtctcctc gtttgcgatc tcgctcggcc
      901 cgatcttctg gctgctgaac gcggagatct acccgctctc ggtcaggagc aaggcggcct
      961 cggccggcac catgaccaac tggttcttca acttcctggt ctcgctgacc ttcctgccgc
     1021 tgatcgacct gctttcacaa accggcgcct tctggttcta cggcgcgatc ggcctggtca
     1081 ccctctggtt ctgctggaag tacgtcccgg agaccaaggg acgcagcctc gagcagatca
     1141 acgacatctt cgaacgccga gcccgataac ccgggctcca gcgttcgcac cgggagtcgg
     1201 gcgggttcgg acccgccctc ccgctgaaac ccgcccctcc cacttgaagc aacggatggt
     1261 ctggaggggc ttcgccttta ggattacggc atgtcgcaga ccccctcctg ctcgattcgg
     1321 gacttccgga tcgaggacgc tgaagcggtt caccgctggt tcaacaacaa ggaagcaacc
     1381 aaaacgctga tggaacagcg tgagtccttc ggcatggaga acgccgaagg gtggacccgg
     1441 gcggcgatga agcaggaggg cgaggaccgc aagtacgcga tcgaggtgcc ggggatcgag
     1501 cagccggtcg gcttcaccgc gctttacgga ctcttccgcc agaccgcacc cgaactcggt
     1561 tgcctgatcg gtgacgaggt gcggggcaag ggggtcggcc gctgggccga acggctgacc
     1621 gtgaccaagg ctttcgagga gttcggcgcc caccggatct acggtcgcat cccggccttc
     1681 aacgaagccg cgaagaaggt cgtgaccgca cagggctgga cctatgaggg caacatgccc
     1741 ggccacatcc ggcggccgga cggcaccatg ctcgactgcg agatctgggg cgccacgccg
     1801 gaacagttct tcgccgcggt cgggaagtgg tgagcgtggc cccgaggttc agttgaggct
     1861 cgccgcggaa ctggtcgccg atggtggccg cgccgccagc ggacccgact tcttccgctc
     1921 ggccgagttt ctcgaagccg agggcactac ccactcgctg ctcatcttcg aggacggcga
     1981 cgaaaccgaa ctggtcgcct ccaccccgct gatcgtccgc gagatccccg gaagcgaccg
     2041 gctcgacgcg atctcgccct acggataccc tggcctcgcg atctccgacg g
//
LOCUS       DAOQFI010000355         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31643_length_2089_cov_3.299410, whole genome shotgun sequence.
ACCESSION   DAOQFI010000355 DAOQFI010000000
VERSION     DAOQFI010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31643_length_2089_cov_3.299410"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..422)
                     /locus_tag="PLE93_06720"
     CDS             complement(54..422)
                     /locus_tag="PLE93_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86775.1"
                     /translation="MIKRAFAVLAAALVLGVAGGAMASTASATIIPREGHYSGHDHNH
                     NHIGLNYTVHGQMNHFTIDYSFVIGGAHVSNAMWHPTCHNGYCTSGAWVNKTTIQGHY
                     WHGGNPHHKVPFIVHWIHSR"
     gene            complement(517..699)
                     /locus_tag="PLE93_06725"
     CDS             complement(517..699)
                     /locus_tag="PLE93_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86776.1"
                     /translation="MADSSEDIQREFEEFEQAETSLDSGLESAPGRDRMPSWIKWIVI
                     ADLVIVIVVALIVFAA"
     gene            complement(718..1071)
                     /locus_tag="PLE93_06730"
     CDS             complement(718..1071)
                     /locus_tag="PLE93_06730"
                     /inference="COORDINATES: protein motif:HMM:NF025590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HNH86777.1"
                     /translation="MIQSPRRALVLAAVLASAFALSACEKEVSIGDNTIDRDELELES
                     SRTLTKQYGEKPASIDCPDDLKAEVDATETCELVDQSGNHYDMKVTVTSVDDDGNAKF
                     NIKVGGIKQMGSVPE"
     gene            1145..1816
                     /locus_tag="PLE93_06735"
     CDS             1145..1816
                     /locus_tag="PLE93_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86778.1"
                     /translation="MHRHLTPSLVLSVIAVVIALGGTSYAISQLPKNSVGARQLKKNA
                     VTAAKLKKDSVSSAKVKDGSLLKKDFKQGQIPKGQTGAAGATGASGPTGPSGPTGETG
                     ETGAPGSAVAYAYVQDIGDPNQSIPSDTAKNMQSSMFSKSQTNPTGVYCFDLESIWPI
                     KNFSVVAESNYNNLPESDKFATGQIQHNNDFGVGCPNTTDLLVITRDASTGNLVNWFF
                     YLTLN"
     gene            1826..>2089
                     /locus_tag="PLE93_06740"
     CDS             1826..>2089
                     /locus_tag="PLE93_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86779.1"
                     /translation="MTRRANLPHHPGLIAAASLVALVAACLILPVGARAAGATFVTTG
                     LPMPADAAPGADSGYSDFRPSGSPLSANGRYVAFQSDASFQLKG"
BASE COUNT          399 a          676 c          633 g          381 t
ORIGIN      
        1 aagaaaaagg ccggcggact gccccgccgg cctttcctcg aatcacttcc gtgttagcgg
       61 gagtggatcc agtggacgat gaacggaacc ttgtgatgcg ggttgccgcc atgccagtag
      121 tgaccctgga tggtggtctt gttcacccag gcaccgctgg tgcagtaccc gttgtggcag
      181 gtcggatgcc acatcgcgtt gctcacatgc gccccaccga tcacgaagct gtaatcgatc
      241 gtgaagtggt tcatctgacc gtgaaccgtg tagttgaggc cgatgtgatt gtggttgtgg
      301 tcgtggcccg agtagtgccc ctccctggga atgattgtgg cgctggcggt cgatgccatt
      361 gcccctccgg caacgccgag cacgagtgcc gcggcgagga cagcgaatgc gcgtttgatc
      421 aaggatccct ctttctgtag cgggacggac gaaaccccgc gttgatcggt gatccgacta
      481 taaacgaact tccgaacgcc gttcggtaaa ttcgtttcag gcggcgaaaa cgatcagcgc
      541 gaccacgatc acgatgacca ggtcggcgat gacgatccac ttgatccacg aaggcatccg
      601 gtcccggccc ggagccgact cgagcccgga atcaagcgaa gtctcggcct gttcgaactc
      661 ctcgaactcc cgctggatgt cttcgctgga atcagccatg gcgttcgcgg cccggcccta
      721 ttcgggaacc gaacccatct gcttgatgcc gccgaccttg atgttgaatt tggcgttgcc
      781 gtcgtcgtcg accgaggtga cggtcacctt catgtcgtag tggtttccac tctgatcgac
      841 cagttcgcag gtctcggtcg cgtcaacctc ggccttgagg tcgtcgggac aatcgatcga
      901 agcgggcttc tccccgtact gcttggtcag ggtgcgtgaa gactccagct cgagctcatc
      961 ccggtcgatc gtgttgtcac cgatcgaaac ttccttctca caggcgctga gggcaaaggc
     1021 cgacgcgagc actgcggcca ggacgagggc acggcgggga gactggatca tgcgcccacc
     1081 ctaccggccg acggggcggc tggaatccct tgccctccgc cgccggcggc cgtagattgg
     1141 ggtcatgcac aggcacctga caccgtcact cgtcctttca gtaatcgccg tggtgatcgc
     1201 cctgggagga accagctacg cgatcagcca gctaccgaag aattcggtcg gggcccgaca
     1261 gctgaagaag aacgcggtga ccgccgccaa gctcaagaag gactcggtca gttcggccaa
     1321 ggtcaaggac ggctccctgc tgaagaagga cttcaagcag ggccagattc cgaagggaca
     1381 gaccggtgcc gccggtgcga ccggggccag cgggccgacc ggacccagcg ggccgaccgg
     1441 cgagaccggg gagaccggag cccccggttc ggccgtggcc tacgcctacg tccaggacat
     1501 cggtgatccc aaccagtcaa tcccctccga taccgcaaag aacatgcaga gctcgatgtt
     1561 ctccaagtcg cagacaaacc cgaccggggt ctactgcttc gacctcgagt cgatctggcc
     1621 gatcaagaac ttctcggtcg tggccgaatc gaactacaac aacctgcccg aaagcgacaa
     1681 gttcgcgacc ggccagatcc agcacaacaa cgacttcggg gtcggctgcc cgaacacgac
     1741 cgacctgttg gtcatcaccc gggatgcgag cacgggcaac ctcgtcaact ggttcttcta
     1801 cctcaccctc aactaggggc cggtcatgac aaggagagca aaccttccgc accatccggg
     1861 tctgatcgcc gcggcgtcac tcgtcgcgct ggtcgctgcc tgcctgattc ttccggtcgg
     1921 cgcccgggcc gccggcgcga ccttcgtcac gaccgggctt ccgatgcctg ccgacgctgc
     1981 acccggggcc gattccggct acagcgactt ccgcccttcc ggcagtccgc tcagcgcgaa
     2041 cggccgttac gtggccttcc agtccgacgc aagcttccag ctcaaggga
//
LOCUS       DAOQFI010000356         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31735_length_2086_cov_2.830625, whole genome shotgun sequence.
ACCESSION   DAOQFI010000356 DAOQFI010000000
VERSION     DAOQFI010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31735_length_2086_cov_2.830625"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..719)
                     /gene="rplJ"
                     /locus_tag="PLE93_06745"
     CDS             complement(177..719)
                     /gene="rplJ"
                     /locus_tag="PLE93_06745"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNH86780.1"
                     /translation="MSKSVKNLIIREYQARLQGFDDAALISIRGVSSNDTNKLRKTLR
                     DKQIKVSVIRNALARKAFEGTPLANLGPLLEGSSALAYGGASVVEVARELVAILESMP
                     TVELKGAVLDGTLYKGKAGVTELSKFPTRVEAQAQAVTLLLGPGRSLVGQVLGPGRTV
                     AGLVKAIETKLEKGEAISKK"
     gene            complement(791..1489)
                     /gene="rplA"
                     /locus_tag="PLE93_06750"
     CDS             complement(791..1489)
                     /gene="rplA"
                     /locus_tag="PLE93_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNH86781.1"
                     /translation="MAIKLSKRKKHNESLKPQAALDPLPACEAIKKFKAPKFDQTVNV
                     VLNLGIDPKQADQAIRGSVSLPKGIGKTKRVVAFCREDQIGPSKANGAVEAGAEDLVA
                     KIEAGWMDFDVAIASPDMMRVVSKLGKVLGPKGLMPSPKSGTVTPNVADAVKEYSAGK
                     VEYRNDAGSNVHAVIGKMSFGASDLAANLEHFIHHIERSRPSTAKGTFIKKVVISGSM
                     TPGVEVKVAQKVVE"
     gene            complement(1494..1922)
                     /gene="rplK"
                     /locus_tag="PLE93_06755"
     CDS             complement(1494..1922)
                     /gene="rplK"
                     /locus_tag="PLE93_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNH86782.1"
                     /translation="MGKKEVVKVFKIYAPGGKATPAPPLGPALGGAGVNPGQFVQQFN
                     AATAKLNGKVVGCVITSYSDRSFTFEIKSSPTAVLIKEAAKIETGSGVPNKNKVGKLT
                     RSQLEAIAKEKMADLSAGSMQAAMRTIEGQCRQMGITVEG"
BASE COUNT          322 a          705 c          652 g          407 t
ORIGIN      
        1 tgtcaccgag ttcctgaatc gacatggttc aaatccttcc aaccgcaaac acgcgtgaga
       61 gagggcccgg aacacccgga gctttaaccc gcgaggggcc agtctgacgc cgatgttgtc
      121 ttgtctgcgc cgcgagagag attccactct cgggcgttga tttcaccaac gcgggctcat
      181 ttcttcgaga tcgcctcgcc cttttcgagc ttggtctcga tggccttgac caggccggcg
      241 acggtccgcc cggggccgag cacctgaccg accaggctgc gaccgggccc cagcaggagg
      301 gtgacggcct gggcctgagc ctcgacgcgg gtcgggaact tgctcagttc cgtgacgccg
      361 gccttgccct tgtacagcgt gccgtcgagc accgcgcctt tgagttcgac cgtgggcatg
      421 gactcgagaa tcgccacgag ttcacgggcg acctcgacca cgctcgcacc gccgtaggcc
      481 agggcgctgg aaccctcgag cagcggcccg aggttcgcca ggggcgtgcc ctcgaaggcc
      541 ttgcgggcca gggcgttacg gatcaccgag accttgatct gcttgtcgcg gagcgtcttg
      601 cgcagcttgt tggtgtcgtt gctgctgacg ccgcggatgc tgatcagcgc ggcgtcgtcg
      661 aagccctgca gacgggcctg atactcgcgg atgatcaggt tcttgactga cttgctcatg
      721 ctggatgctc cttcggagcc ggatgctctg attcagaacc cgccgcgcgc cggagcgacg
      781 cggcccgggc tcactcaacc accttctgcg cgaccttgac ctcgacgccc ggggtcatgg
      841 acccggagat caccaccttc ttgatgaacg tgcccttggc cgtgctgggg cgggaacgct
      901 cgatgtggtg gatgaagtgc tcgaggttgg ccgccagatc ggaggccccg aagctcatct
      961 tcccgatgac cgcgtgcacg ttcgagcccg cgtcgttgcg gtactcgacc ttgcccgcgg
     1021 aatactcctt gaccgcgtcg gcgacgttcg gcgtaaccgt gccgctcttg ggcgagggca
     1081 tgaggccctt ggggcccagc accttaccga gcttggaaac gacgcgcatc atgtccggag
     1141 acgcgatggc gacgtcgaaa tccatccagc cggcctcgat cttggcgacc aagtcctcgg
     1201 cgcccgcctc gaccgcgccg ttggccttcg acggcccgat ctggtcctcg cggcagaagg
     1261 cgacgacgcg cttggtcttg ccgatgccct tgggcagcga caccgacccg cggatcgcct
     1321 ggtccgcctg cttcggatcg atccccaggt tcaggacgac gttcaccgtc tggtcgaact
     1381 tgggggcctt gaacttcttg atcgcctcgc acgccgggag cggatcgagc gccgcctgcg
     1441 gcttgagaga ctcgttgtgc ttcttgcgct tgctcagttt gatcgccatg gattcagccc
     1501 tccaccgtaa tgcccatctg gcggcactga ccctcgatgg tccgcatcgc ggcctgcatc
     1561 gagccggcgc tcaggtcggc catcttctcc ttggcgatcg cctcgagctg cgaacgcgtc
     1621 agcttcccca ccttgttctt gttcggcacg cccgacccgg tctcgatctt cgccgcttcc
     1681 ttgatcagaa ccgccgtggg cgagctcttg atctcgaacg tgaaggagcg atccgagtac
     1741 gaagtgatga cgcagcccac aaccttgccg ttgagcttgg ccgtcgccgc gttgaactgc
     1801 tgaacaaact gaccggggtt gacacccgca ccaccgagcg cagggcccag cggcggcgcc
     1861 ggggtcgctt tcccgcccgg cgcgtagatc ttgaagacct tgactacttc ttttttgccc
     1921 atcgtgctcc acctcctacc ggcccgggac gcttgtccct cctacacctc ctggctaggg
     1981 agtcgtgcag gctcggtagt caacggttga aaatgtcccc gccgcagcgg tccgccgacg
     2041 gacccgagcg ccgaacccgg cgctctgcga ggggcagaag gttagg
//
LOCUS       DAOQFI010000357         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_31974_length_2078_cov_2.814632, whole genome shotgun sequence.
ACCESSION   DAOQFI010000357 DAOQFI010000000
VERSION     DAOQFI010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31974_length_2078_cov_2.814632"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLE93_06760"
     CDS             complement(<1..592)
                     /locus_tag="PLE93_06760"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNH86783.1"
                     /translation="MPKDAQGNAASVRNRDQLGAQGIGELRLKALQIAEAGLAACDGG
                     AATRKRVRLTPDGVMIDGVLHRLDADQRLLVLGGGKATLPVAQALEEVLGDRIDGGAI
                     VLKRGQEAELEGLEVMTSDHPFPSPDSLAAAGKIAGLAAEACEGDLVITCFTGGRSAL
                     LTLPPETVTFDEKRRLHELLIASGMPITAINTVRKHV"
     gene            complement(626..1603)
                     /locus_tag="PLE93_06765"
     CDS             complement(626..1603)
                     /locus_tag="PLE93_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNH86784.1"
                     /translation="MPTADSGFLNRIRSFSISPETYQVVTLIALVVLVIIVFTGAAVR
                     LTASGLGCPDWPKCYGGVVAPPQINAWIEYGNRLVTGVVSITVIAAALLAFLRKPYRW
                     HLAFFGILLPLGVIGQAILGAFVVYYHLPPELVIFHFILSMILIDAAFALFWCSRYEP
                     GERRYSTDWVSTWAVRALIPIGQLTIFLGTITTASGPHPGDHDEELVRRFDFKGTDTL
                     EWVVQRHAAIAVIFGLSVLAVIFIIRRQGGDRRAQKPLIVTLGLICLQLAVGITQWLL
                     HLPAGLVWVHVVVATLLWLAVLWSVAAAGQITPGTKGALIRKPEAAPTG"
     gene            complement(1590..>2078)
                     /locus_tag="PLE93_06770"
     CDS             complement(1590..>2078)
                     /locus_tag="PLE93_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH86785.1"
                     /translation="AAARLAGVDDDTIKGIVRAQVHRPQVVNGNYPGVTVPANLLEGN
                     DLEDVAAYVAAVAGVPGIEPPKAPGEGAGAQIYANNGCGSCHTLAAAQSAGTLGPNLD
                     EVLPKMSKAEIHEAIVNPGAKVENGYPNAMPSDYASKISEEQLNQLIEFLMTSAAADA
                     NG"
BASE COUNT          361 a          643 c          733 g          341 t
ORIGIN      
        1 acacatgctt gcggacggtg ttgattgccg tgatcggcat gcccgatgcg atcagcagct
       61 cgtgaaggcg cctcttctcg tcaaaggtga ctgtttcggg aggcagcgta agcagcgccg
      121 aacgaccgcc ggtgaagcag gtgatcacca ggtcgccttc gcaggcctcc gcggccagcc
      181 cggcgatctt gccggcggcg gcaagcgagt ctggcgaggg aaagggatgg tcggacgtca
      241 tcacctccag cccttcgagt tctgcttcct gaccgcgctt caggacgatg gcaccaccgt
      301 cgatccggtc tccgagcact tcctccaggg cttgggcaac cgggagggtc gccttgccgc
      361 ccccgaggac taaaagtcgc tggtcggcgt caagccggtg aaggacaccg tcgatcatca
      421 cgccgtccgg agtgagcctc acccttttgc gggttgcggc gccgccgtca caggcggcga
      481 gtccggcttc ggcgatctgc aacgccttga ggcggagctc gccgattccc tgtgcgccga
      541 gctgatcccg attgcgaacc gaagcggcgt ttccctgcgc gtccttcggc atcagcgagc
      601 aatcactggg aggtcagccg gggtttcagc cggtgggcgc cgcttcgggc ttgcggatca
      661 gcgccccctt ggtgccggga gtgatctgac cggccgcggc caccgaccag aggaccgcca
      721 gccacagcag ggtcgcgacc acgacatgga cccagaccag gcccgcgggc aggtgcagca
      781 gccactgggt gataccgacc gccagctgga ggcagatcag gcccagggtc acgatcagcg
      841 gcttctgggc gcgacggtcg cctccctgac gccggatgat gaagatcaca gcgaggaccg
      901 agaggccgaa gatcaccgca atcgccgcgt gtcgctgcac gacccactcg agtgtgtcgg
      961 tgcccttgaa gtcaaagcgc cggaccagct cctcgtcgtg gtctcccgga tggggaccgg
     1021 aggcggtcgt gatcgtgccg aggaagatgg tgagctggcc gatcgggatc agcgcccgta
     1081 ctgcccaggt gctgacccag tcggtcgagt agcgcctttc gccgggctcg taacgggaac
     1141 accagaagag cgcgaaggcg gcgtcgatca ggatcatcga gaggatgaag tggaatatga
     1201 ccagctccgg ggggaggtgg tagtagacga cgaacgcgcc gaggatggcc tggccgatca
     1261 ctcccagcgg caggaggatc ccgaagaagg cgaggtgcca gcgatagggc ttgcgcagaa
     1321 aggccagcag cgcggcggcg atgacggtga tcgagacgac gccggtgacc aggcggttgc
     1381 cgtattcgat ccaggcgttg atctggggcg gcgcgaccac cccgccgtag cacttgggcc
     1441 agtccgggca gccaagcccg gaagcggtca ggcgcaccgc cgccccggtg aagacgatga
     1501 tgacaaggac gaccagcgcg atcagggtta cgacctgata ggtctccggc gagatcgaga
     1561 aactgcggat ccggttcagg aagccggaat cagccgttgg catcagccgc agctgatgtc
     1621 atcaggaact cgatcagctg gttcagctgc tcttcgctga tctttgatgc gtagtcggaa
     1681 ggcatcgcgt tcgggtagcc gttttcaact ttcgcccccg ggttcacgat cgcctcatgg
     1741 atctccgcct tggacatctt cgggagaacc tcgtcgaggt tcgggccgag ggtgccggcg
     1801 gactgggcgg cggccagggt gtggcagctg ccgcagccgt tgttggcgta gatctgggca
     1861 ccggcgcctt cgcccggagc cttgggcggc tcgatgccgg gcactccggc gaccgcggcc
     1921 acataggcgg cgacgtcttc gaggtcgttg ccttcaagca ggttggcggg cacggtcacg
     1981 ccgggatagt tgccgttgac gacctggggg cggtggacct gggcgcgaac gatgcccttg
     2041 atcgtgtcgt cgtccacacc ggcgaggcgg gccgcggc
//
LOCUS       DAOQFI010000358         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32051_length_2075_cov_3.373762, whole genome shotgun sequence.
ACCESSION   DAOQFI010000358 DAOQFI010000000
VERSION     DAOQFI010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32051_length_2075_cov_3.373762"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="PLE93_06775"
     CDS             <1..1598
                     /locus_tag="PLE93_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH86786.1"
                     /translation="GVAAAGGMDPVEAEKQGARDQEVAAGAMARYGAVQAPAAPAETV
                     PAPAFTSGQAIPAPVSPSQTAPAAGVVTPDSVPPAGPGTYESPVPEPVTEPVSEYAAE
                     PAGGYAPEVAETAPAAFAADDTVYEPAAATPEAEAETSIIHEPSLENPGADAAVGNAA
                     EAFLAGGAAGAAGGLAAARASRPEEPAAEPVEPLEPVIAPEPGMAPEHALADEAPVSP
                     VAGQQAPDAATAAAEERLRAKVAEIKATQEPPAPPPPVEPPAAGIAPEPVPPREGLPG
                     TEISPGLAAVERRLSENSEERDRAIRDAEERLRRVEQRAEDAERRAAFAERLAQLKVE
                     ESEREKRLNDVVNGIDRAEQRAREAEARAAAAEQAAAAALEHSDLSPEPVSAPEPAAP
                     APVAPTPEPSAPAPGPPLAEPYGVAPSAPEPVSEPAAPEPVFPEPVSAPAPAADEPPA
                     PSRKGLFGGSAPAATGGASDVVNLNTATFEELREAGLSVTQATRILAYRERFGGYGSV
                     EDLEKVPGFPADLIESLQGRITI"
     gene            complement(1660..>2075)
                     /locus_tag="PLE93_06780"
     CDS             complement(1660..>2075)
                     /locus_tag="PLE93_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86787.1"
                     /translation="GNPATSVAQSPAGWSAATVLHGEGIELDGKTFYGLGGGIPVTPW
                     DWSFDLEEADAGNMLAGLPAGAVLVVHSPPKGHCDESGNGMSLGSTAILKAIEEKQPQ
                     LAVCGHIHEAWGKESKIGPTEVVNLGPSGRYFDLD"
BASE COUNT          312 a          761 c          727 g          275 t
ORIGIN      
        1 ccggagtggc cgccgccggc gggatggatc cggtcgaggc cgagaagcag ggtgccaggg
       61 accaggaagt tgccgccgga gcgatggcga ggtatggcgc ggttcaggcg ccggctgcgc
      121 cggccgagac cgttcccgcg cccgctttca cctccggaca ggcgatccct gccccggttt
      181 cgccaagcca gactgcgccg gcggccggtg tggtgacccc ggacagcgtg ccccctgccg
      241 gaccagggac ttatgaatcg ccagttcccg aacccgtaac ggagccggtt tcggaatacg
      301 cagcggagcc ggccggcggg tacgccccgg aagtcgcgga aaccgccccg gcggcattcg
      361 ctgcggatga cacggtctac gaaccggccg ccgccacgcc ggaagccgaa gccgaaacca
      421 gcatcattca cgagccttca ctcgagaacc ccggcgccga cgccgcggtt ggcaacgccg
      481 ccgaagcttt cctggctggc ggtgccgccg gagctgccgg cggactggcg gccgcccgtg
      541 ccagccggcc cgaggaaccc gcggcggagc cggtcgagcc acttgaaccg gtaatcgccc
      601 ccgagcccgg gatggccccg gaacatgccc tggccgacga agcgccggtt tcaccggtcg
      661 ccgggcaaca ggcacctgac gccgcaacgg cagccgccga ggaacgcctg cgggccaagg
      721 ttgcggagat caaggccacc caggagccgc ccgccccgcc gccgcccgtc gagcctccgg
      781 ccgccgggat tgcgcccgag cctgtgcccc cgcgggaggg actgcccggg accgagattt
      841 cccccggcct ggctgccgtc gagcgccgcc taagcgaaaa cagcgaggag cgcgatcgcg
      901 ccattcgtga tgccgaggag cgcctgcgcc gggtcgagca acgagccgag gacgcggagc
      961 gccgggccgc ctttgccgag cggctcgctc agctcaaggt cgaggaatcg gaacgcgaga
     1021 agcgcctcaa tgacgtggta aacggaatcg accgggccga gcagcgtgcc cgcgaggccg
     1081 aggcccgggc cgccgccgcg gaacaggctg ccgctgccgc cctcgaacac agtgatttga
     1141 gcccggaacc tgtctccgcg cccgaaccgg cggcgcccgc accggtcgcc ccgacccctg
     1201 agccttcggc tccggctccc gggccgccgc tcgccgagcc ctacggcgtg gcgccctcgg
     1261 ctcccgagcc ggtttccgaa cctgccgccc cggaaccggt cttccccgag ccggtctccg
     1321 ctcccgctcc ggccgcggac gagccgccgg ccccttcacg caagggtctc ttcggcggtt
     1381 cggcgccggc cgccaccggc ggcgcctccg atgtggtcaa cctgaacacg gcaacgttcg
     1441 aggagctgag ggaggccgga ctttcggtca cccaggcgac ccggatcctc gcctatcgcg
     1501 aacgcttcgg cggttacggc tcggtcgagg atctcgagaa ggtccccgga ttcccggccg
     1561 acctgatcga gagccttcag ggccggatca cgatctgaca gggagtcggg cggggtccga
     1621 ttcgccctcc aggggtggtc gggcaggccc ggttcgaggt cagtccaggt cgaagtaccg
     1681 gccggagggg cccaggttga cgacttcggt cgggccgatc ttcgattcct tgccccaggc
     1741 ttcgtgaatg tggccgcaga cggccagttg cggctgcttc tcctcgatcg ccttgaggat
     1801 cgcggtcgaa ccgaggctca tgccgttgcc ggattcgtcg caatggccct ttgggggtga
     1861 atggaccacc agcacggcac cggccggcag gccggccagc atgttcccgg cgtcagcttc
     1921 ctcgaggtcg aagctccagt cccagggggt caccgggatg ccgccaccga gcccgtagaa
     1981 ggtcttgccg tcgagctcga tcccctcgcc atggagaacg gtcgccgcgg accagccggc
     2041 ggggctctgt gccacggacg tggccgggtt cccgg
//
LOCUS       DAOQFI010000359         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32150_length_2072_cov_2.879524, whole genome shotgun sequence.
ACCESSION   DAOQFI010000359 DAOQFI010000000
VERSION     DAOQFI010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32150_length_2072_cov_2.879524"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLE93_06785"
     CDS             <1..335
                     /locus_tag="PLE93_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86788.1"
                     /translation="SSVKLTSGQAGQAGSEYEVITGFAGRDMKLSYRTVEVDRPSKVV
                     FESSTGMAKIRDTIEFSGDEKGCEVKYDARILTNGLAKVIDPLFSLIFKRVGDRAAAS
                     LRKALGAR"
     gene            364..1629
                     /locus_tag="PLE93_06790"
     CDS             364..1629
                     /locus_tag="PLE93_06790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH86789.1"
                     /translation="MSRPPVAVVGAGLAGLKCAGLLADAGHEVLVFEASDGVGGRVRT
                     DEVDGFLLDRGFQVLLAAYPEVPRTFDLEALDPGEFDPGALVRLNGRFSPLLDPLRRP
                     AKTIAAVSSPVIGIADKLRVARMRQELLGKEPAEVLSRPDRTALESLKGRGFSPNAIE
                     RFFRPFFGGVFIDPDLGTSSRLMEIFFRCFSAGPAVLPKDGMGQLAASMAARLPEGSL
                     QLGSPVREVRADGVLPTDGDWIEALAVVVATDERSASRLAGLPEPEPGRVTSCVYFDA
                     PAGEISGRTLYLSPRAAGPINELAVPSAVSAGYAPAGRSLVSVSAIGENARRDDLEDA
                     VSAQLGEWFGAATVAGWRHLGTRTVEDALPDFGPGRFRAGGQDPALDSGLFVCGDYRE
                     SPSIQGALVSGRKAAAAVRAKLARARTEG"
     gene            complement(1742..>2072)
                     /locus_tag="PLE93_06795"
     CDS             complement(1742..>2072)
                     /locus_tag="PLE93_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576296.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNH86790.1"
                     /translation="VGGLRVLGANTGGSKHGVLTDSPETLTNDFFVNLLSFETEWKPA
                     DEDSTTFEAPGQGWTGTRVDLVFGSNSELRALAEVYASEDAKDKFVTDFIFAWTKVMN
                     ADRFDLD"
BASE COUNT          317 a          663 c          708 g          384 t
ORIGIN      
        1 cctcatcggt gaaactgacc tccggccagg ccggccaggc cggttccgaa tatgaggtga
       61 tcaccggctt cgccggccgg gacatgaagc tctcctaccg gacggtggag gtggatcgtc
      121 cctcgaaggt cgtcttcgaa tcttcgaccg gcatggcgaa gattcgcgac acgatcgagt
      181 tcagtggaga cgaaaaaggc tgcgaggtga agtacgacgc ccgcatcctg accaacggct
      241 tggccaaggt gattgacccc ctcttctcgc tgatcttcaa gcgggtcggc gaccgggctg
      301 ccgccagtct gcggaaggcc ctgggcgcgc gttgagcatg cccgcgcaag gagccgggcg
      361 ccggtgagca ggccgccggt agccgtcgtc ggggccgggc tcgccggcct gaaatgcgcc
      421 gggcttctcg ccgatgccgg ccatgaagtt ctcgtgttcg aagcctcaga cggggtcggg
      481 ggccgggtcc gcaccgacga ggtcgatggc tttctgctcg accgcggctt ccaggtgctg
      541 cttgccgctt acccggaggt tccccggacc tttgatctcg aggcccttga cccgggtgaa
      601 ttcgatccgg gggcgctggt ccggctaaat ggaaggtttt cgccactgct tgatcccctc
      661 cgccgccccg ccaagacgat cgccgcggtg agttcgccgg tgatcggcat tgccgacaag
      721 ctgcgcgttg cccgcatgcg tcaggagctg ctaggcaaag agccagccga ggttctttcc
      781 cgtcctgacc ggaccgcact cgaatccctg aagggcaggg gcttttcacc gaacgcgatc
      841 gaaaggttct ttcgcccctt tttcggcgga gtgttcatcg acccggacct ggggacttca
      901 tcgcgcctca tggagatctt cttccgctgc ttttcggccg gtcccgcggt cctgccgaag
      961 gacggcatgg ggcaactggc cgccagcatg gccgcgcggc ttccggaagg ctcgcttcag
     1021 ctcggctccc cggttcgcga ggttcgggcc gatggcgtcc tgcccaccga cggggactgg
     1081 atcgaagcgc tagcggtggt agtcgcgacc gacgagcgtt ccgcttcgag attagccggc
     1141 ctgcccgagc ccgaaccggg cagggtgacg agttgtgtct acttcgacgc tcccgctggc
     1201 gagataagcg gcagaaccct ttatctctcg ccgagggccg cagggccgat caacgagctc
     1261 gccgttccca gtgcggtcag cgcgggttat gccccggcag gaaggtcgct ggtttcggtc
     1321 agcgcgatcg gggaaaacgc ccggcgtgac gacctcgagg atgctgtgag cgcccagcta
     1381 ggcgagtggt ttggagctgc cacggtagcc ggctggcgcc acctcgggac tcgcaccgtc
     1441 gaggacgcgc tgcccgactt cgggccgggc cgcttccggg ccggcggcca ggatccggca
     1501 cttgattcgg ggcttttcgt ctgcggggat taccgcgagt cgccctcgat ccaaggagct
     1561 cttgtctcgg gccgcaaggc ggccgcggcg gtgcgggcaa agctggctcg cgccaggacc
     1621 gaaggctgaa cctcgcgcag aaacctcttt gccggaggtt tctgcgctcg gtcggtgcaa
     1681 atcgagatgg cagtttgggg cgataatggg ccacttctgc catctcgatc cctgcggttg
     1741 atcagtcgag gtcgaagcgg tccgcgttca tcaccttcgt ccaggcgaag atgaagtcgg
     1801 tcacgaactt gtccttcgcg tcctcggagg cgtagacctc ggccagggcg cggagttccg
     1861 agttcgagcc gaagaccagg tcaacccggg tgccggtcca gccctggccg ggggcctcga
     1921 aggtggtcga gtcctcgtcg gccggcttcc attccgtctc gaaggagagg aggttcacga
     1981 agaagtcatt cgtgagggtt tccgggctgt cggtcaggac gccgtgcttc gagccgccgg
     2041 tgttggcccc cagcacccgc aggccgccga cc
//
LOCUS       DAOQFI010000360         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32169_length_2071_cov_3.192956, whole genome shotgun sequence.
ACCESSION   DAOQFI010000360 DAOQFI010000000
VERSION     DAOQFI010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32169_length_2071_cov_3.192956"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLE93_06800"
     CDS             <1..742
                     /locus_tag="PLE93_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNH86791.1"
                     /translation="TPTSLAGKQAGLTLSGNFGSTGVLLTGSGTQAFVNVPGDITFPT
                     TIVGKQVTWDESLISNGSAPFSLGSLTIEGADASMFSVETTGSCASVAHGDECPYSIT
                     YKPTSTGSHEAQLKITGNLAGGSRTIPLSGTARNLTPAKVSLKLTGAKQVKPGKTVTL
                     TARISKSGEEQTGDLTLKTVVPKKMAASVKPVRIPHLGAGNRTVTRKITIKVRRSAKS
                     GQKLKVEATVSGGGLNKAGKAGKTVTIR"
     gene            complement(735..1145)
                     /locus_tag="PLE93_06805"
     CDS             complement(735..1145)
                     /locus_tag="PLE93_06805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNH86792.1"
                     /translation="MAAETTHRLRVRYNECDRVGIVFNSNYLVYTDVAVTEFWREHLG
                     GYSEFNEIGLDMVLAEANLRFRQPLEFDDEFDIRVSVGGMTARSMTIEFEFTRSGEAI
                     AEIEISYVCVSSTEHTPQPIPDSIRTVLEGDSAT"
     gene            complement(1135..2046)
                     /locus_tag="PLE93_06810"
     CDS             complement(1135..2046)
                     /locus_tag="PLE93_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH86793.1"
                     /translation="MTRQDQEKKLTVDLPQGTVAFRDEGEGPVLLFSHGIFVDSSLWD
                     GLVERLVPAGFRCVRPETPMGAHRIPLNPETPLSPPRVADLLADFIDALDLGPVTVVG
                     SDSGGAVSQMFAAQHPDKLAAMVLTNCDALEVYPPFPFTLLPPLARMPGGIAPLGLLM
                     KLRPVRWATYRMLTADPIPDRQLKAWLGPTGSNPGIRRDVNKVIGGLEKNQAIDAAEK
                     LRGFEKPVLFAWGESDRFFKPELAERLAAMIPNSRIEKVEGGRTFVALDDPEKVSNLI
                     VEFMNETVAPAGRTDAQRSTLAGADGR"
BASE COUNT          400 a          672 c          664 g          335 t
ORIGIN      
        1 cacccccacc tcgctggccg gcaagcaggc cggcctgacc ctctcgggca acttcggcag
       61 cacgggagtg ctcctgaccg gctccggcac ccaggccttc gtcaacgtcc cgggtgacat
      121 caccttcccg acaacgatcg tgggcaagca ggtgacctgg gacgagtccc tgatttccaa
      181 cggttcggcc ccattcagcc tcggctcatt gaccatcgag ggagccgacg cgtcgatgtt
      241 ctcggttgag acaaccggca gttgcgcgag cgtcgcccac ggcgatgaat gcccgtacag
      301 catcacctac aagccgacct cgaccggaag ccacgaagcg cagctgaaga tcaccggaaa
      361 cctggcaggg ggatcccgga cgatcccgct ctccggcacc gccaggaacc tgactccggc
      421 gaaggtctcg ttgaagttga ccggagccaa acaagtgaag cccggaaaga ccgtgaccct
      481 gaccgccagg atctcaaaga gtggcgagga gcagaccgga gacctgaccc tcaagacggt
      541 ggttccgaag aagatggccg cctcggtgaa accggtcagg atcccccacc tcggagcagg
      601 caaccggacc gtcaccagga agatcacgat caaggtcagg aggagcgcga agagcggcca
      661 gaagctgaag gtcgaggcga ccgtgtccgg cggcggcctg aataaggccg gcaaggccgg
      721 gaagaccgtg acgatcaggt agccgagtca ccctcaagca cggtgcggat gctgtccgga
      781 attggctggg gagtgtgttc ggttgacgag acgcagacgt aggagatctc gatctcggcg
      841 atcgcctcgc cgctccgggt gaattcgaac tcgatcgtca tcgagcgcgc ggtcatgccg
      901 cccacgctca cccggatatc gaactcgtcg tcaaactcga gcggctgccg gaagcggagg
      961 ttcgcctcgg ccaggaccat gtcgaggccg atctcgttga actccgaata accacccagg
     1021 tgctcgcgcc agaactcggt aaccgccacg tcggtgtaga cgaggtagtt cgagttgaag
     1081 acgatcccga cccggtcgca ctcgttgtaa cggaccctca gcctgtgggt ggtttcagcg
     1141 gccatcagct cccgcgagag tacttcgttg cgcatccgtc cgaccggccg gggcgaccgt
     1201 ctcgttcatg aattcgacga tcaggttcga gaccttctcg gggtcgtcga gggcgacgaa
     1261 ggtcctgccg ccttcgacct tctcgatgcg ggagttcggg atcatcgcgg ccagccgctc
     1321 ggccagttcc ggcttgaaga aacggtcgct ttcaccccag gcgaagagca ccggcttctc
     1381 gaatccccgt agcttctcgg cggcgtcgat cgcctggttc ttttccagcc cgccgatcac
     1441 cttgtttacg tcccggcgga ttccgggatt gcttccggtc gggcccagcc acgccttcag
     1501 ctggcgatcc gggatcggat cggcggtcag catccggtag gtggcccagc gcaccggcct
     1561 gagcttcatc agcaggccga gcggggcgat tccaccaggc atccgggcga gcggcggaag
     1621 cagcgtgaag ggaaacggcg gatagacctc gagtgcatcg cagttggtga gcaccatcgc
     1681 cgccaacttg tcgggatgct gcgcggcaaa catctgactg acggcaccgc cggaatcgct
     1741 gccaaccacg gtcaccggac cgagatcgag cgcatcgatg aaatcagcga gcaggtccgc
     1801 cacgcggggc ggggagagag gcgtctcggg gttgagcgga atccggtggg cacccatcgg
     1861 agtctccggc cgcacgcagc gaaacccggc cggaaccagg cgctcgacca gcccgtccca
     1921 gagcgatgag tcgacgaaga tcccgtgcga gaagaggagc accggcccct ccccctcgtc
     1981 ccggaatgcg accgtgccct ggggcaggtc gacggtcagt ttcttttctt ggtcttgccg
     2041 ggtcatcggt cctcctcagg ttgcgtaccg c
//
LOCUS       DAOQFI010000361         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32212_length_2070_cov_2.410422, whole genome shotgun sequence.
ACCESSION   DAOQFI010000361 DAOQFI010000000
VERSION     DAOQFI010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32212_length_2070_cov_2.410422"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PLE93_06815"
     CDS             <1..1135
                     /locus_tag="PLE93_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNH86794.1"
                     /translation="IIEAASVLNSDGGVTVAATAIRGDRWQIELTRFSGGNGKPAFCR
                     GRRANVLGTSGDDVLKGGYDSVIVAGAGDDKVRAGSKAVICLGSGDDELRAGDDADKR
                     PLVFGGSGNDRIKSGGRIIGGPGRDEINATSSARVFAGPGDDDVRAFGGFEINGGAGD
                     DTLKARYGGRLNGGPGNDHLIGSDTVGDLTDILDGGPGDDRLEGNGRPDRLFGRAGDD
                     ALDGGTGPDLLVGGTGRDQIDPGPPIADRQIYAVDNRKARGRVWINGNQWSAGDVQVW
                     YRCSGWNYGTVRDSFHGSSTLNEDLKFNSRGRAKVDFDFWDGFGFDSDGWMRARKTER
                     RIFVAYWGREDFSYGEDGSFKCRTGLLKFSLRRVAGERDIVRP"
     gene            1283..>2070
                     /locus_tag="PLE93_06820"
     CDS             1283..>2070
                     /locus_tag="PLE93_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH86795.1"
                     /translation="MNTRFHVPSKVFGTRRSRGERPDGDPGSQSETSVVPASISGTDP
                     LLIDPMLNGSPGKSCSYLIQGEKNVLIDPGSANAAPRIIEALKSNRIKVDLILLTHTH
                     PDAAAGVGVVAREFPNAEIASPAGATEQLADPSAMVSGMRKVYGEKVEKLYGLPAPVD
                     SIRIHAVGDGEQVDLGDRAIEAIATPGHTAAHMSYLDPESSSLFCGDALGIQLPGSSV
                     VRPSTPPWDFSLEDSLDSIERLAGVEAETVYLAHYGAARPGPTE"
BASE COUNT          386 a          628 c          705 g          351 t
ORIGIN      
        1 aatcatcgag gctgcctcgg ttctgaactc cgacggcggc gtgaccgtgg cggctacggc
       61 gatccgggga gatcgctggc agatcgaact gaccaggttc tcgggcggga acggcaagcc
      121 ggccttctgc cgcggccgcc gggcgaatgt tctgggaacc tccggtgacg acgtgctcaa
      181 gggaggctac gactccgtga tcgtggccgg tgcgggtgac gacaaggtgc gtgcgggctc
      241 gaaagcagtg atctgcctcg gctccggtga cgacgagctg cgagccggcg atgatgcgga
      301 caagcgaccg cttgtcttcg gaggatccgg aaacgaccgg atcaagtccg gcgggcggat
      361 catcggcggt cccggcaggg acgaaatcaa cgccacatct tcggcgaggg tattcgccgg
      421 accgggcgat gatgacgtca gggctttcgg cggtttcgag atcaacggcg gtgcgggtga
      481 cgacaccctg aaagcccgtt atgggggacg gctcaacggc ggcccgggaa acgaccacct
      541 gattggcagt gacacggtcg gtgacctgac cgacattctc gacggcggtc cgggcgatga
      601 tcgcctcgaa ggcaacggcc gccccgatcg tctcttcgga agggcaggcg acgacgccct
      661 cgacggcggt accggccccg acctgctggt cggcggcacc ggtcgcgacc agatcgaccc
      721 gggcccgccg atcgcggacc ggcagatcta cgcggtcgac aaccggaagg cgagaggacg
      781 cgtatggatc aacggcaacc agtggtcggc gggagatgtc caggtctggt accggtgcag
      841 tggctggaac tacggaaccg tccgggacag cttccacgga tcctccactc tcaacgaaga
      901 cctgaagttc aacagccggg gccgggccaa ggtcgatttt gacttctggg atggatttgg
      961 cttcgattcc gatggctgga tgagagcacg gaagactgag cgcagaatct tcgtcgccta
     1021 ttggggccgg gaagattttt cctatggcga agatggctcc ttcaagtgcc gaaccgggct
     1081 tctgaagttc tcgctgcggc gtgtggcggg cgagcgggac atcgtgcgtc cctgaagttc
     1141 ggatcgaacg aacctgacgc cggggtcaac ggggcgtccg caggggtccg gcattgtggt
     1201 tctcgacaaa cgttaagtgg gtatttagag ggaaaaccgc atccatctgg aagatggttg
     1261 gttagagaac tcagaggatg agatgaacac gcgcttccac gtcccgagca aggtctttgg
     1321 cacacgccgc agccgcggcg agcgacctga cggtgacccc ggaagccagt ccgaaacctc
     1381 ggtggtcccc gcctcgatct ccggcaccga tccgctgctg atcgatccaa tgctgaacgg
     1441 gagcccgggc aagtcctgct cctacctgat ccagggcgag aagaacgtac tgatcgaccc
     1501 gggatcggct aatgccgcgc cccggatcat cgaggcactg aaatcgaacc ggatcaaggt
     1561 cgacctgatc ctcctcacgc acacccaccc ggacgcggcc gccggggtcg gtgtcgtcgc
     1621 ccgcgaattc ccgaacgcgg aaatcgcctc gccggccgga gcgaccgagc agcttgccga
     1681 tccctcggcc atggtttccg ggatgcgcaa ggtctacggc gaaaaggtcg agaagcttta
     1741 cgggcttccg gcaccggtcg attcgattcg gatccacgcg gttggtgacg gcgagcaggt
     1801 tgatctgggc gaccgggcga tcgaggcgat cgcgactccg ggccacactg ccgcgcacat
     1861 gtcttacctc gacccggaga gttcatcgct tttctgcggg gacgcgctcg gaatccagct
     1921 gcccggcagc agcgtcgtcc ggccgtccac cccgccctgg gatttctcgc ttgaggattc
     1981 gctggattcg atcgagaggc tggccggggt cgaagccgaa acggtctatc tcgcccatta
     2041 cggggcggct cgtcccgggc cgaccgagat
//
LOCUS       DAOQFI010000362         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32287_length_2067_cov_3.446322, whole genome shotgun sequence.
ACCESSION   DAOQFI010000362 DAOQFI010000000
VERSION     DAOQFI010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32287_length_2067_cov_3.446322"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /gene="ftsY"
                     /locus_tag="PLE93_06825"
     CDS             complement(<1..950)
                     /gene="ftsY"
                     /locus_tag="PLE93_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNH86796.1"
                     /translation="MSTTWNDILDLGDRAVPDTAPPPAAEEEAPKKMGAFRRLRESLS
                     KSRRALTEEIGSSLFDRIDDETWERLEEALILADAGATTTAQVVEKLEYEVDSGAVPP
                     DGAAIKERLIGILTEIASTNRAPINLSQDPAVVLMTGVNGTGKTTSIGKIAWHLRNEF
                     GLSVLLGAADTYRAAAAEQLATWAERAGADLVRSNEGADPGSVAFDAINAARSRGSDV
                     VIIDTAGRLHTQSNLMDELGKVRRVIDKQIPGAPHETLISIDATTGQNGLRQAKAFAE
                     ATEVSGVVLTKLDGTAKGGITLAIATELDQEVLCLVDAPD"
     gene            complement(996..>2067)
                     /locus_tag="PLE93_06830"
     CDS             complement(996..>2067)
                     /locus_tag="PLE93_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH86797.1"
                     /translation="SADQTGQAIRDRVARAERTVIGDQGDADEISAELKQCSDLEVEI
                     QGKLRAVAEQVTGAEVEAAHLGDRKAEAAGELARISGILETEIPEPEEPLEDEEREQI
                     EKRLVNLRTRRERLGPVNPLAKKEYEEALEHVEQLVAQREDTERAMRELEGVIRDIDA
                     EIKRCFDETFAATAKNFEEMIAQLFPGGSGRLRSVDLRPVPQPAAEGSEAEGEEEAEE
                     IEDEDGPRTPSESDFGVELEVTPQGKRMRQMSLLSGGEKAMTALAFVFAVFLARPCPF
                     YILDEVEAALDDANINRFLELVRRFSDRTQFVIVTHQKLTMDAADVLYGVSMGGDGVT
                     KVISRRLPKGVTALEGTQLEAA"
BASE COUNT          288 a          684 c          685 g          410 t
ORIGIN      
        1 aaatccggcg cgtcgaccag acagagtact tcctggtcga gctcggtcgc gatcgcgagg
       61 gtgatcccgc ccttggcggt tccgtcgagc ttggtcagga cgactccgga gacctcggtc
      121 gcttcggcga aggccttggc ctggcgcaga ccgttctgac cggtggtcgc gtcgatcgag
      181 atcagggttt cgtgcggggc accggggatc tgcttgtcga ttacccgcct gaccttgccc
      241 agctcgtcca tcaggtttga ctgggtatga agccggccgg ccgtgtcgat gatcacaaca
      301 tcggagccgc gggacctggc ggcattgatc gcgtcgaagg cgaccgaacc cgggtccgca
      361 ccctcgtttg agcgaaccag atccgccccg gcccgctcgg cccaggtcgc aagctgctcg
      421 gccgcggccg cccggtaggt gtcggcggcg ccaagcagga cggacagccc gaactcgttc
      481 ctgaggtgcc aggcgatctt gccgatcgag gtggtcttgc cggtgccgtt gaccccggtc
      541 atcaggacca cggcgggatc ctggctgagg ttgatcggcg cccggttggt cgaggcgatc
      601 tcggtcagga tgccgatcag gcgctccttg atcgccgcgc catccggcgg caccgctccg
      661 gaatcgacct cgtattcgag cttctcgacc acctgggcgg tcgtggtcgc tcccgcgtcg
      721 gccaggatca gcgcttcctc gaggcgctcc caggtctcgt cgtcgatccg gtcgaagagg
      781 ctggaaccga tttcctcggt cagcgcccgg cggctcttcg agaggctttc gcgaagcctt
      841 cggaacgcgc ccatcttctt cggcgcttcc tcctcggcgg cgggtggcgg ggcggtgtcg
      901 gggacggcgc ggtcaccgag gtcgaggatg tcgttccagg tcgtgctcac ggttcggatc
      961 ttgccggaat cgggttgccg gtgtcagaga agcggttacg cggcttccag ctgcgtgcct
     1021 tcgagggcgg taaccccctt cggcaggcga cgggagatca ccttggtcac gccgtcgcca
     1081 cccatgctga ccccgtagag cacgtcggcc gcgtccatgg tcagcttctg gtgagtcacg
     1141 atcacgaact gtgtgcgatc cgagaagcgg cggaccagtt cgaggaaacg gttgatgttg
     1201 gcgtcatcga gggcggcctc gacctcgtcg aggatgtaga acgggcaggg ccgggcgagg
     1261 aagaccgcga agacgaaggc cagcgcggtc atcgccttct cgccgccgga aagcaggctc
     1321 atctggcgca tgcgcttgcc ctgcggtgtg acttcgagtt cgaccccgaa gtcggattcg
     1381 ctcggggtgc gggggccgtc ctcgtcctcg atctcttccg cttcctcttc cccttctgct
     1441 tcggaccctt cggcagcagg ctggggtacg gggcgcaggt caaccgaacg gagccggccg
     1501 gagccaccgg gaaaaagctg ggcgatcatc tcttcgaagt tcttcgcggt ggcggcgaaa
     1561 gtctcatcga agcagcgctt gatctcggcg tcgatgtcac ggatcacccc ttccagttcc
     1621 cgcatcgccc tttcggtgtc ttcccgctgg gcgacgagtt gctcgacatg ctccagcgct
     1681 tcctcgtatt ccttcttggc cagcggattg accggcccga ggcgctcccg gcgggtccgc
     1741 aggttcacca ggcgcttttc gatctgttcg cgctcttcat cttcgagcgg ttcctcgggc
     1801 tcggggatct cggtttcgag gattcccgaa atgcgagcga gctcaccggc cgcttctgcc
     1861 ttgcggtcgc cgagatgggc ggcttccact tccgccccgg tcacctgctc ggccacggcc
     1921 cgcagcttcc cctggatttc gacttccagg tccgagcact gcttgagttc ggccgagatc
     1981 tcgtcggcgt cgccctggtc cccgattacg gtccgctccg cccgggcgac ccggtcccga
     2041 atcgcctgac cggtctgatc agccgag
//
LOCUS       DAOQFI010000363         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32410_length_2063_cov_4.225100, whole genome shotgun sequence.
ACCESSION   DAOQFI010000363 DAOQFI010000000
VERSION     DAOQFI010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32410_length_2063_cov_4.225100"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLE93_06835"
     CDS             complement(<1..838)
                     /locus_tag="PLE93_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNH86798.1"
                     /translation="MPAGDSTDNGNVYLGDSSVITVTVNSDGSSPLDITSVSIYAFPG
                     NSEYFSIEQDNCSGQSVESGDHCTFQVRFTASVVGHKFTSVRIDSNDVESGGMKFFGI
                     SGTGINPGIDADAASHNFGTVQVGEYGTDKTFTIENDGTSDLNATATITGTDAAEFFF
                     SSQPNCASVTPDSTCQIIVNLRPLGSAGPRSANLVIANNTKNENPVTIPLTATASAPH
                     FTSEPLGLGFGQFDLFDDPPATRPLTIKSDGTADLKINGMVIDPLNSNSNAFTLDQGE
                     CVD"
     gene            1042..1467
                     /locus_tag="PLE93_06840"
     CDS             1042..1467
                     /locus_tag="PLE93_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86799.1"
                     /translation="MKKAPLFLVAALASAALLAGGSPAGAALQGSPKPLKVKLVTFDG
                     KQRLKVKRKVRLIASCSKDCNARVKLRLVTPVTTVRGGGSIGLEATGTWTIGVILSPS
                     FRSYIRQNITRCRLVANFSAVDIETGKTVRKVKTFRFRR"
     gene            complement(1457..>2063)
                     /locus_tag="PLE93_06845"
     CDS             complement(1457..>2063)
                     /locus_tag="PLE93_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNH86800.1"
                     /translation="EDQDREINRLVFPRQPKHDRICLADFYRPLNDEGIRDVVALQGV
                     TVGPEVTELMEQLEKDGEFAEQLYTHGLGVQSAEGLAEWLHYKVRQDLGIKSSQGRRY
                     SWGYPACPDQSEHEKVWELLDLGEIGMYLSGGYAVMPEQSTVAIIAHHPQAVYFGMKS
                     GFIPKGEAPDEVIAESDRSANPPEADPDEGPTEAEGEPATA"
BASE COUNT          368 a          614 c          681 g          400 t
ORIGIN      
        1 cgtccacgca ttccccctgg tcgagggtga aggcattgga gttcgagttg agggggtcga
       61 tgaccatgcc gttgatcttc aggtccgcgg ttccgtcgga cttgatggtc aggggcctgg
      121 tcgcgggcgg gtcatcgaac aggtcgaact gaccgaaccc cagtcccaga ggctcgctgg
      181 tgaaatgagg ggcgctggcg gtggcggtca gcgggatcgt gaccgggttc tcgttcttcg
      241 tgttgttggc gatcaccagg ttggcggagc gggggccggc gctcccgagc gggcgaagat
      301 tcacgatgat ctggcaggtg gaatccggcg tgaccgaggc gcagttgggc tgggaactga
      361 agaagaactc ggcagcatcg gtaccggtga tggtcgcggt ggcgttcagg tccgaggttc
      421 cgtcgttctc gatggtgaag gtcttgtcag ttccgtactc accaacctgg accgtcccga
      481 agttgtgact cgcggcatcc gcgtcgatcc ctgggttgat gccggtaccg ctgatgccaa
      541 agaacttcat gccgccactt tccacgtcgt tgctgtcgat cctgaccgaa gtgaacttgt
      601 ggccgaccac ggaagcggtg aacctgacct ggaaggtaca gtggtcgccg gactccaccg
      661 attgacccga gcagttgtcc tgctcgatcg agaaatattc ggagttcccc gggaacgcgt
      721 aaatcgagac cgaagtgatg tcgaggggac tggatccgtc tgaattcacc gtcacggtga
      781 tcacactgga atcaccgagg tagacgttgc cattgtcggt cgagtccccc gcgggcaagc
      841 tcaggtgagg cgcagacgaa gcggcagcag ttggaaccat caatgcgaac gcagcgaaaa
      901 gcgaaactag gaaacccctg gcgaacatcc aaactccctt ggtcggttga gctgatccta
      961 ctttatgaac accgtacgga atgaacgggt gcggtctggt gccttaggct gggaacagtt
     1021 ctcaaccgcc gggaggaatc gatgaagaaa gcgcccttgt ttcttgtggc cgccctggcc
     1081 tcagccgcct tgctcgccgg cgggtcaccc gccggagccg cacttcaagg cagcccgaag
     1141 ccgctcaagg tgaagctggt cacctttgac ggcaagcagc ggctcaaggt caaacggaag
     1201 gttcgcctga tcgcttcctg ctcgaaggac tgcaacgcgc gggtcaagct ccggctggtc
     1261 accccggtga ccaccgtccg cggcggcgga tcgatcggcc tcgaggccac gggcacctgg
     1321 acgatcggcg tgatcctcag tccgtctttc cgcagctaca tccgccagaa catcaccaga
     1381 tgcaggctgg tggcgaactt ctcggcggta gacatcgaaa cgggcaagac cgtacgaaag
     1441 gtcaagacct tccgcttcag gcggtagccg gttcgccctc ggcttcggtc gggccttcgt
     1501 ccggatcggc ctcgggcgga ttggccgagc ggtcggattc ggcgatgacc tcgtccgggg
     1561 cttctccctt ggggatgaaa ccggacttca tgccgaaata gaccgcctgc ggatggtggg
     1621 cgatgatcgc gaccgtggac tgttcgggca tcacggcgta gccgcccgag aggtacatgc
     1681 cgatctcgcc caggtcgagc agttcccaga ccttctcgtg ttcggactgg tccgggcagg
     1741 ccgggtatcc ccatgagtag cggcggccct ggctcgactt gatgccgagg tcctggcgca
     1801 ccttgtagtg gagccattcg gccagcccct ccgccgactg gactccgaga ccgtgggtgt
     1861 agagctgctc ggcgaactcg ccgtccttct cgagttgctc catcagctcg gtcacctcgg
     1921 ggccgaccgt cacgccctgc agggcgacca cgtcgcggat cccttcgtcg ttcagcggcc
     1981 ggtagaagtc agccaggcag atccggtcgt gcttgggctg gcgggggaag acgaggcggt
     2041 tgatctccct gtcctggtcc tcc
//
LOCUS       DAOQFI010000364         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32466_length_2062_cov_2.509716, whole genome shotgun sequence.
ACCESSION   DAOQFI010000364 DAOQFI010000000
VERSION     DAOQFI010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32466_length_2062_cov_2.509716"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLE93_06850"
     CDS             complement(<1..802)
                     /locus_tag="PLE93_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNH86801.1"
                     /translation="MAIGEEIFRPGSSSDGEVRAGILVTGTEVLTATIRDENGPWLSE
                     QLSGLGVELVEILVVADHPGDLARGLENMKSAGIDLIITTGGLGPTADDLTAEVVAAF
                     SGRKMELDEEMEARIAAILARFAANTNLDLSTDALNEANRKQAMIPVGAVALDPVGTA
                     PGLIVPGQAEGDPLVLVLPGPPRELKPMWEAAQKEPMLRQVLDQATRLEKYRLRMFGT
                     PESELAMSLREIEGEGLPFDGLEITTCLRKGEVEVDVRYREAAADAAEQ"
     gene            complement(860..>2062)
                     /locus_tag="PLE93_06855"
     CDS             complement(860..>2062)
                     /locus_tag="PLE93_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNH86802.1"
                     /translation="VRLGDIFGKPKEKDSPLVAWLGKGIDGKPVSTDLARMPHVLVAG
                     TTGSGKSGCVNAVLSSILMNSSPNDVRLVLVDPKQVELNHYETVPHLLTPVVTNPKLA
                     ANVLNNLIAEMETRYGLMKEARARNIEDYNRIVSAEGEPRLPYILCVIDELADLMMVA
                     PADVEASIIRLAQKSRATGIHLLLATQRPSTDIITGTIKVNIPSRIAFAVSSQTDSRV
                     ILDQGGAEALLGQGDMLFRGPGTSKLARIQGAFVTEDEIARITDFWSAQGEPEFEQEL
                     LSEPETMQEETSDAEFDPDQDDLLNEAIMLVVETETASVSMIQRRLRVGYTRAGRLID
                     MLERRGVISGYEGSKPRQVLITHADLPRVLGGGNAGDVEGEPDAEASAGGEIADLPVP
                     SETLDTSD"
BASE COUNT          315 a          721 c          653 g          373 t
ORIGIN      
        1 gctgctcggc ggcgtcggcg gccgcctccc ggtagcgcac gtcaacctcg acctcgccct
       61 tgcgcaggca ggtcgtgatt tccaggccgt caaagggcag cccctcccct tcgatctcgc
      121 gcaggctcat cgccagctcc gactcggggg tgccgaacat gcgcaaccgg tacttctcca
      181 gccgggtggc ctggtccagc acctgccgca gcatcggctc cttctgtgcc gcttcccaca
      241 ttggcttgag ctcccgcggc ggccccggca gcaccaggac cagcgggtca ccttctgcct
      301 gaccgggaac gatcagcccg ggtgcggttc cgaccgggtc gagggccacc gctccgaccg
      361 gaatcatcgc ctgcttgcgg ttggcctcgt tcagggcgtc ggtgctcagg tcaaggttcg
      421 tgttggcggc gaacctggcg aggattgccg cgatccgcgc ctccatttcc tcgtccagtt
      481 ccatcttccg gccggagaag gcggcgacga cctcggcggt gaggtcatcg gcggtcgggc
      541 cgagaccacc ggtggtgatg atcaggtcga tcccggccga tttcatgttt tcgagacccc
      601 gggccaggtc tccgggatgg tcggcgacga ccaggatctc gaccagctcg acccccagcc
      661 cgctcagctg ctccgaaagc cagggcccgt tctcgtcacg gatcgtggcg gtcagcacct
      721 cggtcccggt gaccaggatg ccggccctga cctcaccgtc cgagctgctc cccggcctga
      781 atatctcctc gccaattgcc acggcctgac cctacagccg cggcaccggt tgcggcctcc
      841 gccgaagccc ggcttccggc taatccgagg tgtcgagcgt ctcggacggg accggcaggt
      901 cggcaatttc gccgcctgcg gaagcttcag cgtcaggttc cccctccaca tcgccggcat
      961 ttccgccgcc aagcacccgc ggcagatcag cgtgggtgat cagcacctgg cggggcttcg
     1021 aaccttcgta accggagatc acgccccggc gctcgagcat gtcgatcagg cgcccggccc
     1081 gggtgtaacc gaccctcaga cgcctttgaa tcatcgatac cgaagccgtc tcggtctcga
     1141 ccaccagcat gatcgcctcg ttcagcaggt cgtcctggtc cggatcgaat tccgcgtccg
     1201 aagtctcttc ctgcatcgtc tccggctcgc tgagcagttc ctgttcgaat tccggctcgc
     1261 cctgggccga ccagaagtcg gtgatccggg caatctcgtc ctcggtcacg aaggccccct
     1321 ggatgcgggc cagcttcgag gtaccggggc cgcggaagag catgtcgccc tggccgagca
     1381 gcgcctcggc gccgccctgg tcgaggatca cccgcgagtc ggtctgcgaa gagaccgcga
     1441 aggcgatccg ggagggaatg ttcaccttga tcgtgccggt gatgatgtcg gtcgacggcc
     1501 gctgcgtggc cagcagcagg tggatgccgg tcgcccgcga cttctgggcc agccggatga
     1561 tcgaggcctc gacatcggcc ggggcgacca tcatcaggtc ggccagttcg tcgatcacgc
     1621 agaggatgta gggcagtcgc ggctcgccct cggccgaaac gatccggttg taatcctcga
     1681 tgttgcgggc ccgggcctcc ttcatcaggc cgtaacgggt ttccatctcg gcgatcaggt
     1741 tgttcagcac gttggcggcc agcttcggat tggtcaccac cggggtcagc agatgcggaa
     1801 ccgtctcgta gtgattcagc tcgacctgct tcgggtcgac cagcaccagc cgcacgtcat
     1861 tcggcgagga attcatcagg atcgacgaga gaaccgcgtt cacgcagccg gacttgcccg
     1921 aaccggtggt gccggcgacc agcacatgcg gcatcctggc caggtcggtt gaaaccggct
     1981 tgccgtcgat tcccttgccc agccaggcga ccagcggcga gtccttctcc ttgggcttgc
     2041 cgaagatgtc ccccagccgg ac
//
LOCUS       DAOQFI010000365         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32542_length_2059_cov_3.114271, whole genome shotgun sequence.
ACCESSION   DAOQFI010000365 DAOQFI010000000
VERSION     DAOQFI010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32542_length_2059_cov_3.114271"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLE93_06860"
     CDS             <1..633
                     /locus_tag="PLE93_06860"
                     /inference="COORDINATES: protein motif:HMM:NF037470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HNH86803.1"
                     /translation="AAGSVTPRATKLQAALSDPAEPEDSGPLRALRLGISNFMAKDRI
                     DMSAMARQLDINRATLYRWVGSRDQFLVEMIWHLTATSLGRLDRECEATGPDRIIDVT
                     IDFADEVIGNPGMKHWLATEGESAMSLLTRAGSGYQPRLIAWVEESIERQVDAGNMAL
                     PEGVEPHELAYVIERVMGGYIYLDLITGDEPDARRAEPLLRMLLRSTELA"
     gene            complement(677..1570)
                     /locus_tag="PLE93_06865"
     CDS             complement(677..1570)
                     /locus_tag="PLE93_06865"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HNH86804.1"
                     /translation="MEIVRTPEERFAQVPDFPWEPVYREWEGMRLAHVDEGEGEPVLL
                     VHGEPTWGFLWRKVMGPLLDAGYRCIVPDLPGFGRSDKPVDDEWYSYDRHTAALVSLI
                     EELDLSGLTLVCHDWGGPIGLRTASTEVPERFSRLVAMDTGLFTGYQKMSDNWNHFRD
                     FIASHRDVRVAMPIRGGCATELSDEVVAAYEAPFPDVDYKAGIRAFPPMIPVTPEDAG
                     AAEGQAAARYLMKDERPSLLFWADDDPALPLDPVGRAVQMLFPMAEPLTVIENAGHFL
                     QEDHGEEIGRQIATWLDRTAV"
     gene            1648..>2059
                     /locus_tag="PLE93_06870"
     CDS             1648..>2059
                     /locus_tag="PLE93_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86805.1"
                     /translation="MAEQKSGYLVLVAMIFAVSMTFIDQTIVSISIPEIQKDLSLTST
                     GVQWVINGYLLSLAALFALGGKLADVFGHRRLVVIGVLIFAVSSACCGFTPDSSIDEA
                     WLIFFRVIQGAGAALMFPAALAIVISSFPIRDRGK"
BASE COUNT          327 a          678 c          689 g          365 t
ORIGIN      
        1 gctgccggtt ccgtgacccc gcgagccaca aagctccagg ctgcgctgag cgacccggcc
       61 gagcccgagg attccggccc gctccgggcg ctcaggctcg gcatcagcaa cttcatggcc
      121 aaggaccgga tcgacatgtc ggcgatggcc cgtcagctcg acatcaaccg ggccaccctt
      181 taccgctggg tcgggtcgcg ggatcagttt ctggtcgaga tgatctggca cctgaccgcg
      241 accagcctgg gccggcttga ccgcgaatgc gaggcgaccg ggcccgaccg gatcattgat
      301 gtcacgatcg atttcgccga cgaggtgatc ggcaacccgg gcatgaagca ctggctggcg
      361 accgaggggg agtcggcgat gtcgctgctg accagggccg gttcggggta tcagccgcgc
      421 ctgatcgcct gggtcgagga atcgatcgag cgtcaggttg acgccggcaa catggccctg
      481 cccgaagggg tcgagcccca tgaactcgcc tacgtgatcg agcgggtcat gggcggctac
      541 atctacctcg acctgatcac cggcgacgag cccgacgccc gccgggccga gcccctgctc
      601 aggatgctgc tgcgctcaac cgaactcgcc tgaccatctc ttccggccgc accggctggc
      661 ggggccggtc tgcgggtcag accgcggtgc ggtccagcca ggtggcaatc tggcggccga
      721 tctcctcgcc gtggtcttcc tgcaggaagt ggccggcgtt ttcgatcacg gtcagcggct
      781 cggccatggg gaagagcatc tggacggcgc ggccgaccgg gtcgagcggc agggccggat
      841 cgtcgtcggc ccagaacaga agcgaggggc gttcgtcctt catcaggtac ctggccgcgg
      901 cctgaccctc ggccgcgccg gcgtcctcgg gcgtgaccgg gatcatcggc gggaaggccc
      961 tgatcccggc cttgtaatcg acatcgggga agggtgcttc gtaggcggcg actacctcgt
     1021 ccgaaagctc ggtcgcgcag ccgccccgga tcggcatggc cacccgtacg tcccggtgcg
     1081 aagcgatgaa gtcgcggaag tgattccagt tgtccgacat tttctggtag ccggtgaaga
     1141 ggccggtgtc catcgcgacc agccgggaga aacgctcggg aacctcggtc gatgcggtcc
     1201 gcaggccgat cgggccgccc cagtcgtggc agaccagggt caggccactc aggtcgagct
     1261 cctcgatcag gctgaccagg gcggcggtgt gacggtcgta gctgtaccac tcgtcatcga
     1321 ccggcttgtc ggagcgtccg aagcccggca ggtcaggcac gatgcagcgg tagccggcgt
     1381 cgagcagggg gcccatcacc ttgcgccaga gaaaacccca ggtcggctcg ccgtggacca
     1441 gcagcaccgg ctcgccttcc ccctcgtcga catgggcgag ccgcatgccc tcccactccc
     1501 ggtagaccgg ctcccagggg aaatccggaa cctgcgcgaa gcgttcttcg ggtgtgcgaa
     1561 cgatttccat gcgggcagcc tatccggtag aagctgatgc gtgtcaactt tgtttgagcg
     1621 acgggtcgct tcggtaagtt gggtcagatg gcagagcaga agagtggcta cctcgtcctg
     1681 gtcgcaatga tcttcgccgt gtcgatgacc ttcatcgacc agacgatcgt ctcgatctcg
     1741 atccctgaga tccagaagga cctgagcctc acttcaaccg gggttcagtg ggtgatcaac
     1801 ggctatctgc tgtcgctggc cgcgctcttc gcgctcggcg gcaagctcgc cgacgtcttc
     1861 ggtcatcgcc gattggtcgt gatcggcgtc ctgatcttcg ccgtctcctc ggcctgttgc
     1921 ggtttcacgc cggacagcag catcgacgag gcctggctga tcttcttccg cgtcattcag
     1981 ggggccggtg ccgccctgat gttccccgcc gccctggcga tcgtgatctc ctccttcccg
     2041 attcgggacc gcggcaagg
//
LOCUS       DAOQFI010000366         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32554_length_2059_cov_2.288922, whole genome shotgun sequence.
ACCESSION   DAOQFI010000366 DAOQFI010000000
VERSION     DAOQFI010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32554_length_2059_cov_2.288922"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLE93_06875"
     CDS             <1..782
                     /locus_tag="PLE93_06875"
                     /inference="COORDINATES: protein motif:HMM:NF017758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HNH86806.1"
                     /translation="RVRERMDQLGHSPSRIKDATGLAGGLGMLLFARSQPDTPGKLAA
                     HAYSYEHMEIAAYGILAAYADRSGDRETAELARRIESEERAMAGRISERFGEAVSAAL
                     IGRTTTLDRLLARYVANLHAIEAQGRQLCARTMGMVDHPELAGILNRQLRDGWVQQAV
                     WKRDLEDLGDGPSTLKDLPLALGGNFVATFLLAQPDPNTKVAGFAFAFQNLLAGSCEM
                     LERVARLAGEDTIAAQAESRAAAVRNSANRISKLWEQLGGV"
     gene            810..1130
                     /locus_tag="PLE93_06880"
     CDS             810..1130
                     /locus_tag="PLE93_06880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNH86807.1"
                     /translation="MSEVVVVAVLEVKEGRAEEAITAFTPVIEATHREPGCVSYALHQ
                     DNSDPNTLVLVERWKSQDDLNSHFTQPHMAEMGSLAAELLTGQPRIVFCSGIPKGDPA
                     KGVL"
     gene            1181..>2059
                     /locus_tag="PLE93_06885"
     CDS             1181..>2059
                     /locus_tag="PLE93_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH86808.1"
                     /translation="MEKGRGETVIIGGGIHGVSTALALASRGRKSLILDAAAPLEGAS
                     VHNEGKVHLGFVYALDEHGATTRSMVEGALAFAPLIERWCGEVDWAGLRSSPFAYLVM
                     NEGLAGAGQLEAHYESVLAEIGSAGASFGSNYLGVDPGRAEIVRHHGVFPGMAAGHSQ
                     CWFETPERSVRPVALAALLKRAAVDERAIEILPGHRVTRARRREAGFELEVETPEGPR
                     SIGAETVVNCAWEGRPALDRMILDQPSATNFRVKYSVIIHGEDNGKVGTATLAQGPFG
                     DVLPWPGGDIYISWYPA"
BASE COUNT          365 a          644 c          710 g          340 t
ORIGIN      
        1 agcgggtccg ggagcggatg gatcagctgg ggcactcgcc gtcgcggatc aaggatgcga
       61 ccggcctcgc cggtggcctg gggatgctcc tgtttgcccg ctcccagccg gacacacctg
      121 gcaagctcgc ggcccacgcc tactcgtatg aacacatgga gatcgctgcg tacgggattc
      181 tcgcggccta cgccgatcgc tccggagacc gggagaccgc cgagctcgcc cgccggatcg
      241 aatccgaaga gagggcgatg gctggacgga tatcggagcg tttcggtgaa gcggtatcgg
      301 ccgctctgat cggacggacc accacgctcg atcgcctgct cgcccgctac gtagccaacc
      361 ttcacgcgat cgaagcccag ggccggcagc tttgtgccag gaccatgggg atggtcgacc
      421 atccggaact ggccggcatc ctcaaccgtc agctccggga cggctgggtc cagcaggccg
      481 tgtggaaaag ggatctcgag gatctcggtg acggaccgtc caccctcaag gacctgccgc
      541 tcgcgctcgg cggcaacttc gtcgcgacct tcctcctcgc tcagcccgac ccgaacacga
      601 aggtagccgg gttcgcgttc gccttccaga acctgctggc tggatcctgc gagatgctcg
      661 agcgggtcgc gcgactcgcc ggcgaggaca cgatcgccgc ccaggccgag tcgcgtgccg
      721 ctgccgtcag gaactcggcg aaccggatct cgaaactttg ggagcagctc ggaggggtct
      781 gaccgtcgat tcccggatag ggtcgggtca tgagcgaagt ggtcgtggtg gcggtactgg
      841 aagtcaagga gggaagggcg gaggaggcga tcaccgcgtt taccccggtg atcgaagcga
      901 cgcaccggga acccggctgc gtttcgtatg cccttcatca ggacaactcg gacccgaaca
      961 ccctggtcct ggttgaacgc tggaagtccc aggacgacct gaattcacac ttcacccagc
     1021 cgcacatggc cgagatggga tcgcttgccg ccgaactgct caccgggcaa ccgcggatcg
     1081 tcttctgctc cggaatcccg aagggagatc ccgccaaggg ggttctctag cctcgctcag
     1141 gccccttggc acccggccgg gaaggaatac gctggcggca gtggagaagg ggagagggga
     1201 aacggtgatc atcgggggcg ggattcacgg ggtctcgacc gcgctggcgc tggcctccag
     1261 gggccggaag tcactgatcc tggatgcggc ggcgcctctc gaaggcgcga gtgtgcacaa
     1321 cgaagggaag gtccacctcg ggtttgtcta tgccctcgac gagcacggag ccaccacccg
     1381 ctcgatggtc gaaggagcgc tcgccttcgc tccgttgatc gagaggtggt gcggtgaagt
     1441 cgactgggcg ggcctgcgtt cgagcccgtt cgcatatctg gtcatgaacg aaggcctggc
     1501 cggagccggt cagctcgaag cccattacga atcggtgctg gccgagatcg ggagcgctgg
     1561 agcttccttc ggaagcaact acctgggggt cgatcccggc cgggcggaga tcgttcgtca
     1621 ccacggggtt ttccccggga tggccgctgg tcactcgcag tgctggtttg aaactccgga
     1681 aaggtcggtg cgtccggttg cgctggccgc tctcctgaaa cgggcggcgg ttgacgaacg
     1741 ggcgatcgaa atcctcccag gacacagggt cacccgggcg cggcggcggg aagccggatt
     1801 tgaactcgag gtggaaacgc ctgaagggcc ccggtcgatc ggagcggaaa cggtggtgaa
     1861 ctgcgcctgg gaaggccggc cggccctgga ccggatgatt ctcgaccagc cttcggccac
     1921 gaacttccgg gtcaaatact cggtgatcat ccacggggaa gacaacggaa aggtcgggac
     1981 ggccaccctc gcgcagggtc ccttcggtga cgtccttccc tggcccggcg gcgacattta
     2041 catcagctgg tatccggcc
//
LOCUS       DAOQFI010000367         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32601_length_2057_cov_3.433067, whole genome shotgun sequence.
ACCESSION   DAOQFI010000367 DAOQFI010000000
VERSION     DAOQFI010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32601_length_2057_cov_3.433067"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLE93_06890"
     CDS             complement(<1..290)
                     /locus_tag="PLE93_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86809.1"
                     /translation="MAILAVAGLGVIATGCGDSSSDSVQDTIDKANETANKALDQANK
                     QLDQANKQLDQANQNAQDQLDQAQQKLDNQGAQQKVDDAQKQLDDLQKQVNDA"
     gene            396..1091
                     /gene="tsaB"
                     /locus_tag="PLE93_06895"
     CDS             396..1091
                     /gene="tsaB"
                     /locus_tag="PLE93_06895"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNH86810.1"
                     /translation="MIGFDTATDFTVVGATDAGRPIFERSLPPARRPLHSQALLENVT
                     GAVESLGGWDAVERIAVGIGPGTFTGIRIGVATAAGLAASTGIPVTGVPTLAALARSI
                     GEATGAGTVLPLLDARRGEVFGGLHDGRGEPLEEPFACGPEELTERLSGPGRSGPAPM
                     VGGSGAIRFRDHLVQAGLSLEPDESAVHRLTGRSICELGAAAETTGPENLQPLYLRVP
                     DAQLWLERDGKKV"
     gene            1066..1590
                     /gene="rimI"
                     /locus_tag="PLE93_06900"
     CDS             1066..1590
                     /gene="rimI"
                     /locus_tag="PLE93_06900"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNH86811.1"
                     /translation="MNETARKSDSRNSGLSVRELAYADLPLIISIERRSFRSPWSVGM
                     FALEMSKVDTIGLAAVENDRVVGYLVLSRFDRAWHLMNVAVAPEHRRRGIASLLITDA
                     LKQIEEDEPVTLEVRPTNRSAIELYVGLGFRSYGLRRGYYPDNGEDALIMWKGDPEAA
                     GVPRESIADLPTGA"
     gene            1592..>2057
                     /locus_tag="PLE93_06905"
     CDS             1592..>2057
                     /locus_tag="PLE93_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNH86812.1"
                     /translation="MPLLAIETSCDDTCAAVLDGSRILSNVISSQADHERYGGVVPEV
                     ASRHHLELAVPVVEAALADAGVGFDDLDSVAVTAGPGLIGALLVGLSTAKAIAAPRRL
                     PLVPTDHLHGHVAANFLDPDPLEPPFLCLIASGGHTMLAGVRELNQVHQQAVG"
BASE COUNT          303 a          679 c          690 g          385 t
ORIGIN      
        1 gcgtcattga cctgcttctg gaggtcgtcg agctgcttct gggcgtcatc gaccttctgc
       61 tgcgcgccct ggttatccag cttctgctgg gcctggtcga gctggtcctg ggcgttctgg
      121 ttcgcctgat caagctgctt gttggcctgg tcgagctgct tgtttgcctg atcgagtgcc
      181 ttgttcgcgg tctcgttcgc cttgtcgatc gtgtcctgga ccgaatccga cgaggaatcg
      241 ccgcagccgg tggcgatgac gccaaggcca gctactgcga gaatcgccaa ggacgtgccg
      301 agtttcctgc ttccgatcat caattcgtct ccagtcgtta tttctgcggt ggactttctg
      361 ctggtttcgc cagcctatag gggtccgcgg tgaaaatgat cggcttcgac accgcgaccg
      421 atttcacggt ggtcggcgcc accgacgccg gacggccgat cttcgagcgg agcctgcccc
      481 cggccaggcg cccgctccac agccaggccc tgctcgagaa cgtcaccggg gcggttgaaa
      541 gcctgggtgg ctgggatgcg gtcgagcgga tcgcggtcgg aatcggtccc ggcaccttca
      601 ccggcatccg gatcggtgtc gccaccgcgg ccgggctggc cgcatcgacc ggcatcccgg
      661 tcaccggcgt cccgaccctt gccgctttgg cccgttcaat cggagaggcg accggagccg
      721 gaaccgtgtt gccgctgctc gacgcgcgcc gcggagaggt cttcggcgga ctccacgacg
      781 gccggggcga gccgcttgag gaaccgtttg cctgcgggcc ggaagagctg accgaacggc
      841 tgagcgggcc gggccggagc ggaccggcac cgatggtcgg gggatccggg gcgatacgat
      901 tccgcgacca tttggttcag gccgggttgt ctttagagcc ggacgaatca gcggtccacc
      961 ggctgaccgg gcgctccatc tgtgaactgg gagcggccgc cgaaacaacc ggaccggaaa
     1021 atctccaacc cctctacctg cgagtacccg atgcccaact ctggcttgaa cgagacggca
     1081 agaaagtctg actcccggaa ttccgggctc agcgtccgcg agctggccta cgcggacttg
     1141 ccgctgatca tctcgatcga gcgtcgctcg ttccgcagcc cctggtcggt tggcatgttc
     1201 gcgctggaga tgtccaaggt ggacacgatc gggctcgcag cggtggagaa cgaccgggtg
     1261 gtcggctacc tcgtgctctc gcgcttcgac cgggcctggc acctgatgaa cgtggcggtc
     1321 gcgcccgagc accggcgtcg cgggatcgct tcgcttctga tcactgatgc cctgaagcag
     1381 atcgaggaag acgagccggt gacgctggaa gtcaggccga ccaaccgttc ggcgatcgag
     1441 ctttacgtcg gcctcgggtt ccgttcctac ggcctgcgcc ggggctatta cccggacaac
     1501 ggtgaagacg ccctgatcat gtggaagggt gacccggagg ccgccggagt tccgcgggaa
     1561 tcgatcgcgg acctgcccac cggggcctga aatgccgctg ctggcgatcg agacttcctg
     1621 cgacgacacc tgcgccgcgg tgctcgacgg ttcgcggatc ctctcgaacg tcatttcctc
     1681 gcaggccgat cacgagcgct acgggggagt ggtccccgag gttgcgtcac ggcatcacct
     1741 cgagctcgct gtgccagtgg tcgaagccgc gctggccgat gcgggggtcg ggttcgacga
     1801 cctcgactcg gtcgcggtca ccgccgggcc cgggctgatc ggggccttgc tggtcggtct
     1861 cagtaccgcc aaggcgatcg ccgcgccgcg gcgactgccc ctggtcccga ccgaccatct
     1921 gcacggccat gtcgccgcca actttctcga tcccgacccg ctcgagccac ccttcctctg
     1981 cctgatcgct tccggcggtc acaccatgct ggccggggtg cgggagctca atcaggtcca
     2041 tcagcaggcg gtgggcg
//
LOCUS       DAOQFI010000368         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32795_length_2050_cov_4.350877, whole genome shotgun sequence.
ACCESSION   DAOQFI010000368 DAOQFI010000000
VERSION     DAOQFI010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32795_length_2050_cov_4.350877"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLE93_06910"
     CDS             complement(<1..278)
                     /locus_tag="PLE93_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86813.1"
                     /translation="MKPGPGNARERAAEIAVEKPGISRSLTLDWQDPTASAAEGSKLA
                     GLEYMVAVMDGTIPPAPIAVLMNMTPTEVEEGRAVFFALPGEEHYNPIG"
     gene            561..1709
                     /locus_tag="PLE93_06915"
     CDS             561..1709
                     /locus_tag="PLE93_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH86814.1"
                     /translation="MNFDLTDEQRLLQNTVRDFARNEVVPVAEELDRTKAFPYEIVAK
                     LGDLGLMGIPFPEEYGGSGGDILSYALAVEELARIDSSVAITMAAHISLGTTPIYNWG
                     TDEQKADWMPELTAGRKLAAFGLTEPEAGSDAGNTKTRAELDGDEWVVNGAKQFITNS
                     GTDISACVTITAVTGKDENGRPEISNIIVPNGTPGYTVDPPYRKMGWNASDTHPLTFE
                     DARVPESNLLGPRGQGFRQFLQILDGGRIGVAAMSVGVAQGALDEAIKYAKERQAFGQ
                     SISKFQSIQAKIADISAQLEAARLLTYRAAIEKDRGENFSLTAAQAKLITGRLAVRAT
                     EEAVQIHGGYGFIEEYPVCRFYRDAKILTIGEGTDEVQQMVIARALGC"
     gene            complement(1738..>2050)
                     /locus_tag="PLE93_06920"
     CDS             complement(1738..>2050)
                     /locus_tag="PLE93_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNH86815.1"
                     /translation="AGRLFDQGQMPDGVYATIDTLARALLDRAASERVAVPGELRIAT
                     CSNGELARTSQPQLTTLDEHPATLGEEAVELLVRAIEDPESAPSFVKVDTELLVREST
                     A"
BASE COUNT          347 a          702 c          641 g          360 t
ORIGIN      
        1 ccgatcgggt tgtagtgctc ctcccccggc agggcgaaga acacggcgcg gccctcttca
       61 acctcggtcg gtgtcatgtt catgagcacc gcaatcggcg cgggtggaat ggttccgtcc
      121 atgaccgcga ccatgtattc gaggccggcc agcttgctgc cttccgccgc cgaggccgtt
      181 gggtcctgcc agtcgagggt gagcgaacgg ctgatcccgg gcttctcaac cgcgatctcc
      241 gccgcccgct ccctcgcgtt accggggccg ggtttcaccg ggtcggcctc cagcgtcggg
      301 gtgcgccctc cggcctgatc ttcccgccaa ccccgtactg caggtcggcg aggatctctg
      361 cctctgtgag gtcgatcttc aggggcctca tctggaatct cgttcggttt ctcggattgc
      421 gcttcattct tgctcccctt tcggggtcgg ttcggccctc cgtagagggt cgcgtctgca
      481 gtccaccata tacgtgtaag tctgatcata agacttagat gagatatctc acacgaggaa
      541 ggaattcgta ggatcgcgtc atgaacttcg acctgaccga cgaacagcgt ctcctccaga
      601 acaccgtccg cgatttcgcc cgtaatgaag ttgttcccgt cgcggaggag ctcgaccgca
      661 ccaaggcctt cccctacgag atcgtcgcca agctcggcga cctcgggctg atgggtatcc
      721 ccttcccgga ggaatacggg ggcagcggcg gcgacattct ttcctacgcc ctcgcggtcg
      781 aggaactggc ccggatcgac tcgtcggttg cgatcaccat ggcggcccac atctcgctcg
      841 gtaccacccc gatctacaac tgggggaccg acgagcagaa ggccgattgg atgcccgagc
      901 tcaccgccgg ccggaagctc gccgccttcg gcctgaccga acccgaagcc ggctccgacg
      961 caggaaacac caagacccgg gccgagctcg acggcgacga atgggtcgtg aacggggcca
     1021 agcagttcat tacaaactcc ggcaccgaca tctccgcctg cgtcacgatc accgccgtga
     1081 ccggcaagga cgaaaacggc cggcccgaga tctcgaacat catcgtgccc aacggcactc
     1141 ccggttacac cgtcgatcct ccctaccgca agatgggctg gaacgcctcg gacacccacc
     1201 ccctgacttt cgaggatgcc cgggttcccg aatcgaacct gctcggcccg cgcggccagg
     1261 gtttcaggca gttcctgcag attctcgacg gcggccggat cggcgtcgcc gcgatgtcgg
     1321 tcggagtcgc ccagggcgcg ctggacgaag cgatcaagta cgcgaaggaa cgccaggctt
     1381 tcggccagtc gatttccaag ttccagtcga tccaggcgaa aatcgccgac atctccgccc
     1441 agctcgaggc cgcccggttg ctgacctacc gggccgcgat cgaaaaggac cgcggcgaga
     1501 acttctccct gaccgccgcc caggccaagc tgatcaccgg ccgcctcgcc gtgcgggcga
     1561 ccgaggaggc ggttcagatc cacggcggtt acggcttcat cgaggagtat ccggtctgcc
     1621 gcttctaccg cgacgcgaag atcctcacga tcggcgaggg gaccgacgag gtccagcaga
     1681 tggtgatcgc ccgggccctg ggctgctgag gcagccgggg agggcttagg gcttcgctca
     1741 ggcggtcgat tccctgacca ggagctcggt gtccaccttc acgaaggagg gcgccgactc
     1801 cgggtcttcg atcgcgcgga ccagcaattc gaccgcttcc tccccgagcg tggccggatg
     1861 ctcgtccagc gtggtcagct ggggctgcga ggtgcgggcc agctcgccgt tgctgcaggt
     1921 ggcgatcctg agctcgccgg gcaccgcgac gcgttctgaa gccgcccggt caagcagggc
     1981 ccgggcgaga gtgtcgatcg tcgcgtagac cccgtccggc atttgcccct ggtcaaatag
     2041 ccggcccgcc
//
LOCUS       DAOQFI010000369         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32801_length_2050_cov_3.141353, whole genome shotgun sequence.
ACCESSION   DAOQFI010000369 DAOQFI010000000
VERSION     DAOQFI010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32801_length_2050_cov_3.141353"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLE93_06925"
     CDS             <1..388
                     /locus_tag="PLE93_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNH86816.1"
                     /translation="VNEPFQCAGGGGGCALVEKSKYANFLGVHMSIWGLIGYATILGA
                     TIWKGDNARLAAFGLALFGFTVSLVLRYLELWEIHAACQWCWASAVLMTVLLVVNSCR
                     LIGYYGTGDPEQQTSFADEAEPDPAS"
     gene            403..1182
                     /locus_tag="PLE93_06930"
     CDS             403..1182
                     /locus_tag="PLE93_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH86817.1"
                     /translation="MATKSQQKRKELRDKRMQAEQAAQSADKRQNLAKILGIAAFLVV
                     VGVVVAVVAISSSGGGDEPKGSGDIDKLLAGIPQNGTTLGEANSKVTLYEFGDLQCPI
                     CEQYSEDATPDIINNIVRPGKAKWNFKQWAILGPDSTLAAKAALAAAEQNRYWEFIEN
                     FYANQGIENSGYVTDDFLTEIANDAGVPDIDKWNADRESAAIAKQVEQTDAQASSLGF
                     SGTPSFGVQVGNGDIEPVQTTSSTAAAGEIAKAIEKAQSGN"
     gene            1238..1795
                     /locus_tag="PLE93_06935"
     CDS             1238..1795
                     /locus_tag="PLE93_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86818.1"
                     /translation="MESRISSWLRCYRCWSRNLEVQVHYDAIRPIDPETGIPTEGDDE
                     IQESVVQCLDCMHDQPHLTFERDRVVPIEDRWERMVAGTPWVASCTVTVEADQVEACA
                     GPEAVESLTFGAFGDAGTREFFTHVRFHKHHEDQIAVHLLVELYARNPEEASEVLTGA
                     ARGTIEITSLAEESRPPAHASGDTH"
BASE COUNT          388 a          674 c          646 g          342 t
ORIGIN      
        1 cgtgaacgag cccttccagt gcgccggcgg cggtggcggc tgcgccctgg tcgagaagag
       61 caagtacgcg aacttcctcg gggtacacat gtcgatctgg gggctgatcg gctacgcgac
      121 gatcctcgga gcgacgatct ggaagggtga caatgcccgg ctggccgcct tcggactcgc
      181 gctgttcggc ttcaccgtca gcctggtcct ccgctatctg gaactctggg agatccacgc
      241 cgcctgccag tggtgctggg ccagcgcggt gctgatgacc gtgttgctgg tggtcaattc
      301 ctgccgtctg atcggttact acggaaccgg agatccggag cagcaaacct catttgctga
      361 cgaggcagag ccagatcccg cctcttagta gcatccccgc caatggccac caaatcgcag
      421 cagaagagaa aagaactccg cgacaagcgc atgcaggccg agcaggctgc gcagagcgcc
      481 gacaagcgtc agaacctcgc aaagatcctc gggatcgcgg cctttctggt cgtggtcggc
      541 gtggtcgtgg cggtcgtcgc gatcagctcc agcggaggcg gcgacgagcc gaagggatcg
      601 ggagacatcg acaagctgct ggccggaatc ccgcagaacg gcaccacgct cggcgaagca
      661 aacagcaagg tgacgctgta cgagttcggt gacctgcagt gcccgatctg cgagcagtac
      721 tcggaggacg ccaccccgga catcatcaac aacatcgtcc ggcccggcaa ggccaagtgg
      781 aacttcaagc agtgggcgat tctcggcccg gattcgaccc tcgctgccaa ggctgcgctc
      841 gccgctgcgg aacagaaccg ttactgggaa ttcatcgaga acttctacgc caatcagggc
      901 atcgagaatt ccggttatgt gaccgacgac ttcctcaccg aaatcgccaa cgacgccggc
      961 gttccggaca tcgacaagtg gaacgccgac cgggagtccg ccgcgatcgc caagcaggtc
     1021 gagcagaccg acgcgcaggc ctcgagcctc ggcttcagcg gcaccccgtc gtttggggtc
     1081 caggtcggca acggtgacat cgagccggtc cagaccacca gttcaactgc ggcagccggc
     1141 gagatcgcca aggcgatcga gaaggcgcag tcgggtaact gacccacata gcgagccaca
     1201 tgccccgccg aggtctccga ggcgcaaaga tatgagcatg gagtcccgta tctcttcatg
     1261 gctcaggtgc taccggtgct ggtcacgcaa cctcgaggtt caggtccact acgacgcgat
     1321 ccggccgatc gacccggaga ccggtatccc gaccgagggc gatgacgaga tccaggaatc
     1381 ggtggtccag tgcctggact gcatgcatga ccagccgcac ctgaccttcg agcgggaccg
     1441 ggtggtaccg atcgaggatc gctgggaacg catggtggcc gggacgccct gggtcgcctc
     1501 atgcacggtc acggtcgagg ccgatcaggt cgaggcctgc gcgggaccgg aagcggttga
     1561 atcgctgacc ttcggcgcct tcggcgatgc cggaacccgt gagttcttca cccacgtccg
     1621 cttccacaag caccacgagg accagatcgc cgtacacctg ctggtcgagc tctacgcccg
     1681 caacccggag gaggcgagcg aagtgctgac cggcgccgcc cggggaacga tcgagatcac
     1741 ctcactggcc gaggagtccc ggccccccgc ccacgcctcg ggcgacaccc attaagaagt
     1801 cgggcggggt ccgacccgcc ctccaccagt aacccgcccc tcccactcat acgatctaat
     1861 tgttgggagg ggctccgtcc ggcggcgcat cgggttaatt gcccaccggc ttgtcccggt
     1921 ctacgacttc tttctttgcc tgttttgcct tttcggcgag tgagccgtcg ccggcgagga
     1981 tctcgcgtag ctccttggcg tcccgcttca ggttctcggg ctcggcccgg tcccccagct
     2041 tgtcctcagc
//
LOCUS       DAOQFI010000370         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32854_length_2048_cov_3.437531, whole genome shotgun sequence.
ACCESSION   DAOQFI010000370 DAOQFI010000000
VERSION     DAOQFI010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32854_length_2048_cov_3.437531"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PLE93_06940"
     CDS             <1..821
                     /locus_tag="PLE93_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH86819.1"
                     /translation="ARVAGLDVANQGAEVRKLIGAALQEAALDPNLTGREHMKLQTAL
                     HGLPREERVRRGDELIERVGLSSAADRRVGGYSGGMKRRLDLALALVHRPRVLFLDEP
                     TTGLDPQSRASLWEEVTRLSTQDGVTVFLTTQYLEEADQLANRVGIIDAGKIAAEGTP
                     SALKAEIGRPSVEVLPVDPARRGEAEQILLRFGTAVPAARDALSVRLNEGINELAAIV
                     RSFDSAGIELASLGLNEPTLDDVFLAKTGRHLEGAGEADSAEMRLPEVEAAGSS"
     gene            818..1654
                     /locus_tag="PLE93_06945"
     CDS             818..1654
                     /locus_tag="PLE93_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH86820.1"
                     /translation="MSGAVATASGSLAGPDSPGPGHSTLPIQVSAMASRSVKRLIRQP
                     ALVIPSVIFPLFLLAVNAGGLNAATHIPGFPTDSYLTFAIGFTFIQAAIFSTMGAGQS
                     LAQDIENGFFSRLVLTPMRPTALMAGQLAGLVLLGQFQALLYLSVGLAAGAHFEAGLA
                     GYFVLAALVSIISIGFGGIGLIMAIRTGSGEAVQGMFPLMFVLLFFSSMTLPRNLIEQ
                     DWYRFLATINPVSYLIEAIRSLFIYGWDTEALALGFGVATVVAVVSIIFAAHSLQGRM
                     LK"
     gene            1651..>2048
                     /locus_tag="PLE93_06950"
     CDS             1651..>2048
                     /locus_tag="PLE93_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86821.1"
                     /translation="MIPDRKYFSAGWAVAWRYLHNLYTKPAVFVPGLIFPLFFLAAFA
                     GGLSAVGDTPKFDYYDFTAFQFCFILIQASALAGVFVGFSIAGDFEQGMGRRMMLATS
                     RRSSILLGYAIGAALRLIVVWVIITGVSLLA"
BASE COUNT          290 a          718 c          671 g          369 t
ORIGIN      
        1 cggcccgcgt ggcggggctc gacgtggcca accagggggc ggaggtgcgc aagctgatcg
       61 gtgccgccct gcaggaagct gccctcgacc cgaacctgac cggccgcgaa cacatgaagc
      121 tccagaccgc gctgcacggg ctccccaggg aggaacgggt ccggcgcggt gacgagctga
      181 tcgaaagggt ggggctttcc agcgcggccg atcgccgggt cggcggctac tcgggaggga
      241 tgaagcggcg cctggacctg gctttggccc tcgttcaccg gccccgggtg ctctttctcg
      301 acgagccgac caccggcctt gacccgcaga gccgtgcctc gctctgggag gaagtcaccc
      361 gcctttccac ccaggacggg gtgacggtct ttctgaccac ccagtacctc gaggaggcag
      421 atcagctggc caaccgggtc gggatcatcg acgccgggaa gatcgccgcc gaaggaactc
      481 cctccgcgct caaggccgag atcggccggc cgagcgtcga ggtgctgccg gtcgatcccg
      541 cacgccgcgg ggaggccgag cagatcctgc tgcgcttcgg caccgcggtt ccggccgccc
      601 gggatgcgct cagcgtgcgt ctcaacgaag gcatcaatga gctggcggcg atcgtcaggt
      661 cattcgattc ggccggaatt gagctggcca gcctcggcct gaacgagccg accctcgatg
      721 acgtcttcct ggccaagacc ggccgccacc tggaaggggc cggcgaggcc gacagcgccg
      781 agatgcggtt gcccgaggtc gaagcggcag gttcctcgtg agcggagccg ttgcaaccgc
      841 gagcgggagc ctggccgggc cggacagccc cggccccggg cattcgacgc tgccgatcca
      901 ggtctcggcg atggcgagcc ggtcggtcaa gcggctgatc cgccagcccg ccctggtaat
      961 tcccagcgtg atcttccccc tcttcctgct cgccgtgaac gcgggcgggc tcaacgcggc
     1021 gacccacata ccgggcttcc cgaccgattc ctacctgacc ttcgcgatcg gcttcacctt
     1081 catccaggcg gcgatcttct cgaccatggg ggcgggccag agcctcgccc aggacatcga
     1141 gaacggcttc ttcagccgcc tggtgctgac cccgatgcgg cccaccgcgc tcatggccgg
     1201 ccagctcgcc ggtctggtcc tgctcgggca gttccaggcg ctgctctacc tctcggtcgg
     1261 tctcgccgcc ggggcgcatt tcgaggccgg tctggccggc tacttcgtgc tggccgccct
     1321 ggtctcgatc atctcgatcg gcttcggcgg gatcggcctg atcatggcga tccgcaccgg
     1381 ctccggcgag gcggtccagg gcatgttccc gctcatgttc gtgcttctct tcttcagctc
     1441 gatgaccctg ccgcgcaacc tgatcgagca ggactggtac cgcttcctcg ccacgatcaa
     1501 cccggtcagc tacctgatcg aggcgatccg cagcctgttc atctacggct gggacaccga
     1561 agccctggcg ctcggctttg gcgtggcgac cgtggtcgcg gtggtttcga tcatcttcgc
     1621 ggcccactcg cttcagggga ggatgctcaa gtgatccccg atcgcaagta cttctccgcg
     1681 ggctgggcgg tcgcctggcg ctacctgcac aacctctaca ccaagccggc cgtgttcgtg
     1741 cccgggctga tcttcccgct gttcttcctg gcagcctttg cgggcggact ctccgcggtc
     1801 ggcgacacgc cgaaattcga ctattacgac ttcaccgcct tccagttctg cttcatcctg
     1861 atccaggctt cggccctggc cggggtcttc gtcggtttct cgatcgccgg tgacttcgag
     1921 caggggatgg gcagacggat gatgctggcc acatcgcggc gctcctcgat cctgctcgga
     1981 tacgcgatcg gcgccgccct gagactgatc gtggtctggg tgatcatcac cggcgtctcc
     2041 ctgctggc
//
LOCUS       DAOQFI010000371         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32919_length_2046_cov_3.601708, whole genome shotgun sequence.
ACCESSION   DAOQFI010000371 DAOQFI010000000
VERSION     DAOQFI010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32919_length_2046_cov_3.601708"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..899
                     /locus_tag="PLE93_06955"
     CDS             186..899
                     /locus_tag="PLE93_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86822.1"
                     /translation="MTAPGKFSIRDAFRGMRLAFEQHRPLLIRVTVLLAALNALSNLF
                     DLTGAAPGFALAVGSFVLLGAVYQGLITALVCLPGRPEGIGELWQAVRPVLGVLIWVT
                     LVYSVVTVAGLFLLVIPGLVIFTIWSVAGQAVVVERKGLFSSLGRSTELISGRGPSVF
                     AFLLLLGIVALSISLLVIVATRPLGDGVLGSLVTPFLTNCLSAPFFVLGVASLYNELS
                     GRGVNPEAARPPDPGPDQP"
     gene            complement(850..>2046)
                     /locus_tag="PLE93_06960"
     CDS             complement(850..>2046)
                     /locus_tag="PLE93_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86823.1"
                     /translation="DRGKAMAIFFGVTGAFTSIGPLAGGYLSEWTWRAIFWINIPVAI
                     LSLIFIWISKPEETKRPQPIDYRGAVLVCIGMALAVLGLQQSSTWGWGDPVTWICIVG
                     GLLVLAFFIRFEMKQEHPLIRVAIFKDRAFAVDNVLLFLLSVAFVPLFFFASMYAQLA
                     LGWETSEAGLYLLIFFAGFATAAQFGGRVLDSRGARPTVILGSLLASVGFVFWAMKLT
                     DLAGGSNAQWPFIVLTGVGFGLLLGPVATDAVNRAPNTSYGEATGITQTVRNYGASVG
                     MAVLGTILILQNKSNIESELAGKVPARTASQIADEVSKAGSGAVTSSAPSGGKAEEIY
                     HSVQQAFAQSSKTVFLCMAGVMALCFVVALIGSPAGRMEEVFADEGEAPKADPDRDPA
                     AGPPRG"
BASE COUNT          329 a          671 c          684 g          362 t
ORIGIN      
        1 agattgaacg gcgtgaattt gagtttgttc tcggaggttt ccatgccggg gagggtttcc
       61 ggttcgatcc cgttcggccc ggtgacggcg acacgatcct cgcgtgtgtc atcccggcca
      121 cgcctgggca gctctctggg gtccgggaac tataaaggca ctccctgcgg gtagggtggg
      181 cgcccatgac cgctcccggc aagttctcga tccgcgatgc cttcagggga atgaggctgg
      241 cattcgagca acaccgtccg ctgctgatcc gggtgaccgt tctgctggcc gcgctgaacg
      301 cgctgtccaa cctcttcgac ctgaccggcg ctgcgcccgg attcgccctc gcggtcggga
      361 gcttcgtcct tttgggcgcg gtctaccagg gcctgatcac cgctctcgtg tgcctgcctg
      421 ggcgacccga gggaatcggg gaactctggc aggcggtgcg ccccgttctt ggcgtgctga
      481 tctgggtcac gctcgtctat tcggtggtga ccgtggccgg gctcttcctg ctggtcatcc
      541 ccggcctcgt gatcttcacg atctggtcgg ttgccggtca ggcggtcgtg gtcgagcgga
      601 agggcctctt ttcatcgctt ggacgcagca cggaactgat cagcggcagg gggccctccg
      661 tgttcgcgtt ccttctcttg ctgggcatcg tcgccctttc gatttcgttg ctggtgatcg
      721 tggcaacccg cccgctcggc gacggcgtgc tcgggagcct ggtcaccccg ttcctcacca
      781 actgcctttc cgccccgttc ttcgtgctcg gggtggcctc gctctacaac gagttgagcg
      841 ggcggggcgt caaccccgag gcggcccggc cgccggatcc cggtccggat cagccttagg
      901 cgcttcaccc tcgtcggcga agacctcctc catccggccg gccggcgagc cgatcagggc
      961 gaccacgaaa cagagcgcca tcaccccggc catgcagaga aagacggtct tgctggactg
     1021 cgcgaaagcc tgctggacgc tgtggtagat ctcctcggcc ttgcccccgg agggcgcgct
     1081 cgaggtgacc gcaccgctgc cggccttcga gacctcgtcg gcgatctggc tggccgtcct
     1141 ggccggcacc ttgccggcca gctcggactc gatgtttgac ttgttctgga ggatcaggat
     1201 cgtgccgagg acggccatgc cgacactcgc cccgtaattc ctgaccgtct gggtgatgcc
     1261 ggtcgcctcg ccgtagctgg tgttcggcgc gcggttgacc gcatcggtgg caaccggccc
     1321 gagcagcagg ccgaagccga cgccggtcag cacgatgaac ggccactgcg cgttggagcc
     1381 accggccaga tcggtcagct tcatcgccca gaagacgaaa ccgaccgagg cgagcaggct
     1441 gccgagaatc acggtcggcc gggccccgcg cgagtcaagg acgcgaccgc cgaactgggc
     1501 cgcggtggcg aaaccggcga agaagatcag caggtagagc ccggcttccg aggtctccca
     1561 gccgagcgct agctgcgcgt acatgctggc gaagaagaag agcgggacga aggcgaccga
     1621 aagcaggaag agcagcacgt tgtccaccgc gaaggcccgg tctttgaaga tcgccacccg
     1681 gatcaggggg tgttcctgct tcatctcgaa gcggatgaag aaggcgagga cgagcaggcc
     1741 gccgacgatg cagatccagg tgaccggatc accccagccc caggtcgaag actgctgcag
     1801 cccgagcacg gccagcgcca tgccgatgca gacgaggacc gcgccccggt agtcgatcgg
     1861 ctgcgggcgt ttcgtctcct ccggcttcga gatccagatg aagatcaggg aaaggatcgc
     1921 cacgggaatg ttgatccaga agatcgcccg ccaggtccat tccgagaggt agccgccggc
     1981 cagcggaccg atcgaggtga acgcgcccgt gacgccgaag aagatcgcca tcgccttgcc
     2041 gcggtc
//
LOCUS       DAOQFI010000372         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_32934_length_2046_cov_2.432948, whole genome shotgun sequence.
ACCESSION   DAOQFI010000372 DAOQFI010000000
VERSION     DAOQFI010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32934_length_2046_cov_2.432948"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="trpS"
                     /locus_tag="PLE93_06965"
     CDS             <1..804
                     /gene="trpS"
                     /locus_tag="PLE93_06965"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNH86824.1"
                     /translation="AILAAGVDPERCILFRQSDIPEHTELCWLLSGVCAHGDLNRMTQ
                     FKEKSAQQKDLVSAGIFIYPLLQAADVLAYQADEVPVGEDQRQHIELMREVARRFNER
                     FGETFTIPEHRIPEIGARVMDLQEPGRKMSTSVGFDSGRVYLLETEKETRKKLGSAVT
                     DSGREVKRGEGKEGVANLVGIYAVATGKTEDEVEAEFEGAGYGDFKKAVADAVVEFLA
                     PVREAYPAIREDEGEIERILADGAAKAREIASVTLADVREAMGVGVPRG"
     gene            885..1646
                     /locus_tag="PLE93_06970"
     CDS             885..1646
                     /locus_tag="PLE93_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNH86825.1"
                     /translation="MASRLDLDLDLDLDVFIGPFDLLLSIVLREEISLLEVELGEIVA
                     MYVGKLEEEGELELEVATEFLVLIAALLELKSRLMLPGADEDLTDMEPAEAVEELVAR
                     MLEYRRYRDAGRALKEHFESQRGFLYRSAPLPPELRRVEVETAEKVYEPEQLAAALGD
                     LLEPPPRPDLSHLRATVSLRRRLSVVRSVLMNRPVFDFDDEFGNEDRMTQAVTIFALL
                     DLYKKGEAVWEQTESCGPITVKRIQAGQNGLARTA"
     gene            1643..>2046
                     /locus_tag="PLE93_06975"
     CDS             1643..>2046
                     /locus_tag="PLE93_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNH86826.1"
                     /translation="MTDLARRIESLLFLSNQPVTEIALAEACEVELEQVQNAVVELEE
                     DLAPGRRGLILRRVGGGLILASDPESEAAARRLLSKPRTPPLTQAQAETLAIVAYLQP
                     VARPEIARIRGVSSESAVNSLSERGLIEESGRS"
BASE COUNT          370 a          658 c          692 g          326 t
ORIGIN      
        1 gcgattctcg ccgccggggt cgacccggag cgctgcatcc tcttccgcca gtccgacatc
       61 cccgagcaca ccgaactctg ctggctgctc tccggagtct gtgcccacgg cgacctcaac
      121 cggatgaccc agttcaagga gaagtcggcc cagcagaagg atctggtttc ggccgggatc
      181 ttcatctacc cgctgcttca ggccgccgac gtactcgcct accaggcaga cgaggtaccg
      241 gtcggcgagg accagcgcca gcacattgaa ctgatgcgtg aggtcgcccg gcgtttcaac
      301 gaacgcttcg gcgaaacctt cacgattccc gagcaccgga tcccggagat cggggcgcgg
      361 gtaatggacc tgcaggagcc aggccgcaag atgtcgacct cggtcggctt cgacagcggt
      421 cgcgtctacc tgctcgaaac cgagaaggaa acgcgcaaga agctcggctc cgcggtgacc
      481 gactccggtc gcgaggtgaa gcgcggtgaa ggcaaggaag gggtcgccaa cctggtcggc
      541 atctacgcgg tggccaccgg caagaccgaa gacgaagtcg aagccgaatt cgagggcgcc
      601 ggttacggcg acttcaagaa ggccgtggcc gatgcggtcg tcgagttcct cgctccggtc
      661 cgggaggcct acccggcgat ccgcgaagac gagggcgaga tcgaacggat cctcgccgac
      721 ggggcggcca aggcccggga aatcgcttcg gtgaccctgg cggacgtgcg cgaggcaatg
      781 ggcgtcggcg tgccgcgcgg atagcgcgaa cgaaggcatc gaagatccgc aaatagcgcc
      841 tttccggagc ggggccgacc gatcgcccct ctaccctttt tgcgatggct tcccggctgg
      901 acctggacct cgatctggat ctcgatgtct tcatcgggcc cttcgacctt ctcctcagca
      961 tcgtgctgcg ggaggaaatc agcctgcttg aagtcgaact cggcgagatc gtcgccatgt
     1021 acgtcgggaa gctcgaggaa gagggcgagc tcgaacttga agtagcgacc gagttcctgg
     1081 ttctcatcgc cgccttgctg gagttgaagt cgcggctgat gctgccgggg gccgatgagg
     1141 acctgaccga catggagccg gcagaggcgg tcgaggaact ggtcgcccgg atgctggaat
     1201 accggcgcta ccgggacgcc ggccgggccc tcaaggagca cttcgaaagc cagcgggggt
     1261 tcctctaccg gtcggccccg ctgccgccgg agctgcgtcg ggtcgaggtc gagaccgcgg
     1321 agaaggtcta tgaaccggag cagctggcgg ccgcgctcgg cgatctgctc gaaccgccgc
     1381 cccggccgga cctctcgcac ctgcgcgcga ccgtgtctct gcgccgtcgc ctttcggtcg
     1441 tccggtcggt cctgatgaac cggccggtct tcgatttcga tgacgagttc ggcaacgagg
     1501 accggatgac ccaggcggta acgatcttcg ccctgctcga cttgtacaag aagggcgaag
     1561 cggtctggga acagaccgaa tcctgcggac cgatcacggt taaacggatt caggccggac
     1621 aaaacggatt ggcgaggact gcatgaccga tcttgcccga cggatcgaat cgctgctgtt
     1681 cctcagcaac cagccggtga ccgagatcgc cctggcggaa gcctgcgaag tggagctcga
     1741 gcaggtccag aacgcggtgg tcgaactcga agaggacctg gcgcccggac ggcgcgggct
     1801 gatcctgcgg cgggtcgggg gagggctgat cctggccagt gatcccgagt cggaagcggc
     1861 cgcccgcagg ctgctctcga agccccgcac cccgccgctc acccaggccc aggccgagac
     1921 gcttgcgatc gtcgcctacc tgcagccggt tgcccggcct gagatcgcaa ggatccgcgg
     1981 cgtttcctcc gagtcggcgg tcaacagcct cagcgagcgc ggcctgatcg aggaatccgg
     2041 tcgctc
//
LOCUS       DAOQFI010000373         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_33034_length_2042_cov_3.044791, whole genome shotgun sequence.
ACCESSION   DAOQFI010000373 DAOQFI010000000
VERSION     DAOQFI010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33034_length_2042_cov_3.044791"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PLE93_06980"
     CDS             complement(<1..1017)
                     /locus_tag="PLE93_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNH86827.1"
                     /translation="MSCTDCDENHPEHPNDAPLPLSEQWKHLHWLQAFWTRAWRENVT
                     GLSGMVAYNLMLAIFPFAFLVLFIFSQVLRVEGIDQGIINDLQRLFPNAEQGTLDDIL
                     KAIRNNSATLGVAAVFGSLWIGASFWGAMDTAFCRIYHVQCRGWWEQKRFSLVMLLVV
                     TIFLLGSVLLPALEGALIRQTDNLPFGLNEVQNLDNAVLLAVTLSLNFTVAAVIYWAV
                     PKGHMPWRAVWPGALFTTLVAAAANWLFPVYLNNVSTLNRFGSTIGFILIALFWFYLV
                     ALTMLAGAVINSIRHEYHDTGSLPYGILSRAQLLARAQADEAKAAQRPMAEPYRSLVS
                     GDAWP"
     gene            1268..>2042
                     /locus_tag="PLE93_06985"
     CDS             1268..>2042
                     /locus_tag="PLE93_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH86828.1"
                     /translation="MLVAELQELEEVKTLVNRGQQLGVLTFGDVATAVAEVDLDESDV
                     EDLYGHIEKQGIELVEDVDPAQKASAENERSDGRRGRRRKKEALDLKPDMTTDSLQLF
                     LKDIGKVRLLTAQEEVDLAKRIERGDLDAKQKMVESNLRLVVSIAKNYRNQGLPFLDL
                     IQEGTLGLVRAAEKFDYRKGFKFSTYATWWIRQAIARALADKARTIRIPVHVVEKLNK
                     IGRAERKLVTELGREPTPEEIAEVTGIDPEEVDSIKRSAQ"
BASE COUNT          419 a          603 c          671 g          349 t
ORIGIN      
        1 cggccaggcg tcaccgctga ccagcgagcg gtagggctcg gccatcggtc gctgtgcggc
       61 cttcgcctcg tcggcctggg ccctggccag cagctgggcc cggctgagga tgccgtaggg
      121 caggctcccg gtgtcgtggt actcgtggcg gatcgagttg atcaccgcgc cggccagcat
      181 ggtcagggcg accaggtaaa accagaaaag ggcgatcagg atgaagccga tcgtcgagcc
      241 gaaacggttc agggtcgaga cgttgttgag gtagaccggg aagagccagt tcgcagcggc
      301 ggcgaccagg gtcgtgaaga gggctcccgg ccagaccgcc cgccagggca tgtggccctt
      361 cggcaccgcc cagtagatca ctgccgcgac cgtgaagttc aggctgagtg tgaccgcgag
      421 caataccgcg ttgtccaggt tctggacctc attcaggccg aagggaaggt tgtcggtctg
      481 acggatcagg gcaccctcga gggcggggag gaggacgctg ccgagcagga agatcgtgac
      541 caccagcaac atgacgagcg agaatcgctt ctgctcccac cagccgcggc actgcacgtg
      601 atagatccgg cagaaggcgg tgtccatcgc cccccagaac gaggcgccga tccagagcga
      661 gccgaagacg gcggccacac cgagggtggc ggagttgttg cggatcgcct tgaggatgtc
      721 gtcgagcgtg ccctgctcgg cgttcgggaa gagccgctga aggtcgttga tgatgccctg
      781 gtcgattcct tcgacccgca gtacctggga gaagatgaac agcacgagga acgcgaacgg
      841 gaagatcgcc agcatcaggt tgtaggcgac catgccggag agcccggtta cgttttcccg
      901 ccaggcccgg gtccagaagg cctgcagcca gtggaggtgc ttccattgct cggagagggg
      961 aagcggggca tcgttgggat gctccggatg attttcgtca cagtcagtgc aactcatggg
     1021 gccggtacgc gttccttgag tgaagcagag ttcacggatc gcagcccttg actttactaa
     1081 gtgaagtggc tattatccgg tcactcgttt gaccacttac agacggcggc ggtttttcct
     1141 gctccggttt acatatggag cgatgcggat cctgacgagt gattcgcaag attttctgtg
     1201 acccggaccc tcattttcgg gtagacaggt atagacgccg tcttacggaa taccagatag
     1261 ggacatgatg cttgtagccg aactccagga actagaagaa gtaaagaccc tcgtcaacag
     1321 gggtcagcag ctcggcgtcc tcactttcgg tgacgttgcc actgccgtgg ctgaggtcga
     1381 tctcgacgaa tcagacgtcg aagaccttta cggtcacatc gaaaagcagg ggatcgagct
     1441 ggtcgaggat gtggatccgg cgcagaaggc ttcggccgag aacgagcgct ccgacggccg
     1501 ccgcggccgc cggcgcaaga aggaggcgct cgacctcaag ccggacatga ccaccgactc
     1561 actgcagctc ttcctcaagg acatcggcaa ggttcgcctg ctgaccgccc aggaagaagt
     1621 cgatctcgcc aagcggatcg agcgcggtga cctcgacgcc aagcagaaga tggtcgagtc
     1681 gaacctccgg ctcgtcgtct cgatcgccaa gaactaccgc aaccagggcc tgcctttcct
     1741 cgatttgatc caggaaggca cgctcggcct ggtccgtgcg gccgagaagt tcgattaccg
     1801 caagggattc aagttctcga cctacgcgac ctggtggatc cgccaggcga tcgcccgcgc
     1861 cctcgccgac aaggcccgga ccatccggat cccggtccac gttgtcgaga agctgaacaa
     1921 gatcggccgt gccgagcgca agctggtgac ggaactgggc cgcgagccca ccccggagga
     1981 gatcgccgaa gtaaccggca tcgaccccga ggaagtcgac tcgatcaagc gttccgccca
     2041 gt
//
LOCUS       DAOQFI010000374         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_33296_length_2035_cov_2.989394, whole genome shotgun sequence.
ACCESSION   DAOQFI010000374 DAOQFI010000000
VERSION     DAOQFI010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33296_length_2035_cov_2.989394"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLE93_06990"
     CDS             <1..432
                     /locus_tag="PLE93_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86829.1"
                     /translation="LLGGDGADWDRAFRWGKRSDLIGQSTARFWRIGATNDAEASSET
                     PSPRSFTFDSGIELNPVLRQRGTISGLDEGTPLAVGVNGRIAAVGQSYEDNGQVRYSI
                     LLPQSTLREGSNRIQLFEVTPFTGGAAGAASLRLLWSSTDQ"
     gene            445..>2035
                     /locus_tag="PLE93_06995"
     CDS             445..>2035
                     /locus_tag="PLE93_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH86830.1"
                     /translation="MTDVAKSGRESGNGGKAPFKRPSLILGAAHLAALWALTFAQPML
                     DLLGENPDFFVARGNSSGQIILYALVLTFAVPLVCLAIEALVRLASRDAQWYLHLFLV
                     GLFGACFVLQLIKNHLDWPAGILIAISIGLAVAGVFIYSRWRFPQAFMDVLSVAPIII
                     LVIFFGFSSTSRLVLPREQPDPVDVKIGTPAPVVMVVFDEFPVGSLMKPDGQIDGTRY
                     PAFAELAGHSTWYRNTSAAAAYTPLAVPAIFSGQKPNNDDLPIASDYPHSVFTLLGKT
                     YDLRVMEAATRICPDTLCPEEDNTAQDGSTSDLFSDLYVVSKYLLLPDSLTRNLPDVS
                     NSFSGFTESDQVDTNAESEVTGLDDGGETGATGTTGLTGATGATGDTQPERTGQGAAR
                     KLGRLFALESSADEFERVGEFNAKLKRGQQETLDLIHIEKPHYPWRHIPNGQRYSNLT
                     AEWSGLLPNDGPWMAPPRIVNIAMQRHLLEVGYTDTLLAQIIKRLKQTGLWERAMVVV
                     TADHGAAFQSKVPRRAATSENM"
BASE COUNT          354 a          717 c          616 g          348 t
ORIGIN      
        1 ctgctcggcg gcgacggagc cgactgggac cgcgccttca ggtggggcaa acggagcgac
       61 ctgatcggcc agtccacggc ccggttctgg aggatcggcg ccacgaacga cgccgaagcg
      121 agctccgaaa ctccctcgcc ccgctcgttt acgttcgatt cggggatcga actgaacccg
      181 gtcctgaggc aacgaggcac gatttccgga ctggacgaag gcacgccgct ggcggttggc
      241 gtcaacggaa ggatcgccgc ggtcggccag tcctacgaag acaacggcca ggtcaggtac
      301 tcgatcctgc tgccgcaatc aacgctccgg gaagggtcca accggattca gttgttcgaa
      361 gtgactccat tcacgggagg tgccgccgga gcagcctcct tgaggcttct ctggtcctcg
      421 accgaccaat agactggcca gtccgtgact gatgtcgcca agtccgggcg cgagtccgga
      481 aacggaggta aagcgccgtt caagcgtccg tccctgattc tgggggcggc ccatctggct
      541 gcgctctggg cgctcacctt cgcccagccg atgctcgacc tgctcggcga gaacccggac
      601 ttcttcgtcg cccggggcaa cagctccggc cagatcatcc tttacgccct ggtcctcacc
      661 tttgcggtgc cgctggtttg cctcgcgatc gaggcgctgg tccgcctggc cagtcgcgac
      721 gcccagtggt atctccacct tttcctggtc ggactgttcg gggcctgctt cgtcctgcag
      781 ctgatcaaga accacctcga ctggccggcc gggatcctga tcgcgatttc gatcggcctg
      841 gccgtggccg gggtcttcat ctattcccgc tggcgctttc cccaggcctt catggacgtg
      901 ctcagtgtcg ccccgatcat catcctggtg atcttcttcg gtttcagcag cacctcgcgc
      961 ctggtcctgc cccgggagca gccggacccc gttgacgtga agatcggaac tccggccccg
     1021 gtcgtgatgg tcgtcttcga cgagttcccg gtcggctcgc tgatgaagcc cgacggtcag
     1081 atcgacggaa ccagataccc ggccttcgcc gagctggccg gccattcgac ctggtaccgg
     1141 aacaccagcg ccgccgctgc ctacaccccg cttgccgtgc cggcgatctt ctccgggcag
     1201 aagccgaaca acgacgacct gccgatcgcc tccgactatc cgcacagcgt cttcaccctg
     1261 ctcggcaaga cctatgacct gagagtgatg gaggccgcca cgcggatctg ccccgacacg
     1321 ctctgtccgg aagaggacaa caccgcccag gacggctcga cctccgacct cttctcggac
     1381 ctttacgtcg tctccaagta cctgctgctg ccggattcgc tgacccggaa cctccccgac
     1441 gtctccaact ccttctcggg cttcaccgag tcggatcagg tcgacaccaa cgctgaatcc
     1501 gaggtgaccg gtctcgacga cggcggtgaa accggggcaa ccggcaccac cggactgacc
     1561 ggagcgacgg gcgctaccgg agacacccag ccggagcgga ccgggcaggg tgccgcccgc
     1621 aagctcggcc ggctgttcgc gctcgaatcc tcggccgacg aattcgagcg ggtcggcgag
     1681 ttcaacgcga agttgaaacg gggccagcag gagaccctcg acctgattca catcgaaaag
     1741 ccccactacc cctggcgcca catcccgaac ggccagcgct actcgaacct gaccgcggaa
     1801 tggtcgggcc tgctgcccaa tgacggtccc tggatggccc cgccgcggat cgtcaacatc
     1861 gccatgcagc ggcacctgct cgaggtcgga tacaccgaca ccctgctcgc ccagatcatc
     1921 aagcggctga agcagaccgg cctctgggag agagcaatgg tcgtcgtcac cgccgaccat
     1981 ggagccgcct tccagtcgaa ggtcccgcgc cgggcggcga ccagcgagaa catgg
//
LOCUS       DAOQFI010000375         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_33497_length_2029_cov_3.218845, whole genome shotgun sequence.
ACCESSION   DAOQFI010000375 DAOQFI010000000
VERSION     DAOQFI010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33497_length_2029_cov_3.218845"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..727
                     /locus_tag="PLE93_07000"
     CDS             173..727
                     /locus_tag="PLE93_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNH86831.1"
                     /translation="MIKPLKYSLRMITRARGSSLLRSGAVAVAAALTFFVLAQSSTVR
                     NQATDLAVGGIGVDTIERATLSLTVIAVIVGALQVGVLMTRVVLQRMQEIGVLKAAGV
                     SSRDIFLVFLFESLIYGFTGGVIGCLVGLLIALLGPMPGSEEILRAFVITVALSTVVA
                     TLAGLAPARRAVQAPAVEAINHAW"
     gene            757..1509
                     /locus_tag="PLE93_07005"
     CDS             757..1509
                     /locus_tag="PLE93_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH86832.1"
                     /translation="MNEHEQVPGGTGPVVETEDLWKLFKLGDEVVKALKGVSLKIHPG
                     ELVVIMGPSGSGKSTMLHLMGGLERPTKGEIYLEGQPLSGLTESQLSTMRHQAVGFIF
                     QSYNLIPLLTATENVELPLVFDDVDSGQVRRDCVELLERVGLGHRLDHTPSRMSGGEQ
                     QRVAIARALIGKPRLLLADEPTANLDHKNGDAIVKLLTTLNHEMGMTTVISTHDRSVA
                     EHASRVIEMRDGKVVSDSGSGDGAPADRLATA"
     gene            1517..>2029
                     /locus_tag="PLE93_07010"
     CDS             1517..>2029
                     /locus_tag="PLE93_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH86833.1"
                     /translation="MTAISSVNPFDPSDQVAGCEGASAAEIDSVLAAGVAAQPGWAAD
                     PMLRLRSLSTLADEIEVRRQKFIDLMVREVGKPVAEAAGEVDRAIAILGFYSQVPLDP
                     VGEVVPGSVPGARVLVERHPLGLVLAICPWNFPLAIPLWKSAPALAYGNAVVIKPASP
                     AIATAALLAEC"
BASE COUNT          312 a          692 c          663 g          362 t
ORIGIN      
        1 tcggcggagg catgttcgcc ctgcttttgc cctcgatctt ctcgctcggg acggtgggca
       61 aggcggttgc cctggtctcg gtctacttcc cgatcatgct cattctcggg gcgttgatcc
      121 cggcctggcg cctgcttcag aagagtcccc tcgaactgct caggagttca ccttgatcaa
      181 acccctcaag tacagcctgc ggatgatcac ccgggcccgc ggctcgtcgc tgcttcgctc
      241 cggcgcggtg gccgtcgcgg ccgccctcac cttcttcgtc ctcgcccaga gttcgaccgt
      301 tcgcaatcag gccaccgatc tcgcggtcgg cgggatcggg gtggacacga tcgagagggc
      361 gaccctttcg ctgaccgtga tcgccgtgat cgtcggcgcc ctccaggtcg gcgtgctgat
      421 gacccgggtg gtgctgcagc ggatgcagga aatcggcgtg ctcaaggcgg ccggagtatc
      481 gagccgggac atcttcctcg tctttctctt cgagtccctg atctacggct tcaccggcgg
      541 ggtgatcggc tgcctggtcg gtctcctgat cgccctgctc ggaccgatgc cgggcagcga
      601 ggagattctg cgggccttcg tgatcacggt cgccctttcc accgtcgttg ccaccctggc
      661 cgggctggcg cccgcccgtc gcgccgttca ggccccggcc gtagaagcaa tcaaccatgc
      721 ctggtgagat gaatgccgcc ggctgaagga gatccgatga acgaacacga gcaagtcccg
      781 ggcggcaccg ggccggtggt cgagaccgaa gacctgtgga agctgttcaa gctcggcgat
      841 gaggtggtca aggcgctcaa gggcgtcagc ctgaagatcc accccggtga actcgtcgtg
      901 atcatgggcc ccagcggcag cggcaagtca acgatgctgc acctgatggg cgggctggag
      961 cggccgacca agggcgagat ctacctcgaa ggccagccgc tctccgggct gaccgagagc
     1021 cagctctcga ccatgcgcca tcaggcggtc ggtttcatct tccagtccta caacctgatc
     1081 ccgctgctga ccgcgaccga gaacgtcgaa ctgcccctgg tcttcgacga cgttgacagc
     1141 ggccaggtcc ggcgtgactg cgtcgagctg ctcgagcggg tcggcctcgg tcaccggctt
     1201 gatcacactc cgagccgcat gtccggcggc gagcagcagc gggtggcaat cgcccgggcc
     1261 ctgatcggca agccgcgcct gctcttggcc gacgagccga ccgcaaacct tgaccacaaa
     1321 aacggcgacg cgatcgtcaa gctgctgacc accctcaacc acgagatggg catgaccacc
     1381 gtgatctcga cccacgaccg ctcggtggcc gagcatgcca gccgggtgat cgagatgcgg
     1441 gacgggaagg tagtttccga ctccggttcg ggggacggcg ccccggccga ccgtctcgcc
     1501 accgcctgag gaagccgtga cagcgatcag cagcgtcaat ccctttgatc cctccgacca
     1561 ggttgccggt tgcgagggtg cttccgcggc cgaaatcgat tcggtcctcg ccgccggggt
     1621 ggcggcccag ccgggctggg cggccgaccc gatgctcagg ctgcgttccc tttcgaccct
     1681 cgccgacgag atcgaagtcc ggcgccagaa gttcatcgac ctgatggtgc gggaagtcgg
     1741 caaaccggtc gccgaggcgg ccggcgaagt tgaccgggcg atcgcgatcc tcggcttcta
     1801 ttcccaggtt ccgctcgatc cggtcgggga ggtggtcccg ggctcggtgc cgggcgcccg
     1861 ggttctggtc gagcgccatc cactcgggct ggtgctggcg atctgcccct ggaacttccc
     1921 cctggcgatc ccgctgtgga agtccgctcc cgccctggcc tacggaaacg cggtcgtgat
     1981 caaaccggct tcaccggcga tcgccaccgc cgcgctgcta gccgaatgt
//
LOCUS       DAOQFI010000376         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_33592_length_2026_cov_3.326230, whole genome shotgun sequence.
ACCESSION   DAOQFI010000376 DAOQFI010000000
VERSION     DAOQFI010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33592_length_2026_cov_3.326230"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLE93_07015"
     CDS             <1..307
                     /locus_tag="PLE93_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S12"
                     /protein_id="HNH86834.1"
                     /translation="GAKARAIKRAQRHWIRGESQPAGPGNNWAGMGLFRYVTKCGVMY
                     GHTGSFPGYRAFAASSADGKRSIAFVANAQIVPGRGSQKVNNRIRRMQVAAVCRALR"
     gene            complement(309..743)
                     /locus_tag="PLE93_07020"
     CDS             complement(309..743)
                     /locus_tag="PLE93_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86835.1"
                     /translation="MSRNLKLTLSSVLVAGLLLAGCGTDKQESVSVSLETFTSPDGNI
                     GCIADQTMVRCDIKHKTWKVKRDPKCQLDYGGGLTVADGKAELVCAGDTTLNSGPDVA
                     AGTVNMVGPFECQTNEWGDSMRCENVTTGNGFELSADKYSTF"
     gene            complement(712..>2026)
                     /locus_tag="PLE93_07025"
     CDS             complement(712..>2026)
                     /locus_tag="PLE93_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNH86836.1"
                     /translation="REFGINAKAVSGETPKRELSDTLAAFERGEVDVLCNAQLLAEGW
                     NSPRATICMHLAPTASKRIYQQRVGRVTRRSPNKEAGLVIDFVHPATTHDESIVTLHS
                     LLDRDVYRGGAIVVGPVRRGRGRKVRVERRIVPVSDDPQKRIEVLERDIWRIAVENLN
                     HSEQHVWAALAGARVTSQGWRRAKAMLQHDRTKELRRRYLLTCVQRNRNAQLRVKALS
                     EIANLKDPEAFDDAIDIVGTWSRDEKRAGTKVLLQALVDKRIGRRDQAQAWVWRLASY
                     TRELHEEYAVQRWPETKRLLGLLVNSAGRAHGKNARKLVQAARKQDRRLAVSLLAAAV
                     AHTPEGEEVLRGARMRMARKPSAVARELLRNFPKGNKKRRRKKNKKKDQAENPEVEST
                     ETTDTSAEGEATGEVEEVQEVDEVDETETTEEAEPDEPESEADPE"
BASE COUNT          278 a          683 c          676 g          389 t
ORIGIN      
        1 aggtgcgaaa gcgagggcga tcaagcgggc ccagcggcac tggatccgcg gcgagtcaca
       61 gccggccggg ccgggcaaca actgggccgg aatgggcctc ttccgttacg tcaccaagtg
      121 cggcgtgatg tacggccaca ccggttcctt ccccggctac cgtgccttcg ccgcctcctc
      181 ggctgacggc aagcgctcga tcgccttcgt cgccaacgcc cagatcgttc ccggccgcgg
      241 ctcgcagaag gtcaacaacc ggatccgccg catgcaggta gcggcggttt gccgcgccct
      301 gcgctgacct agaaggtcga gtacttgtcg gccgagagtt cgaagccgtt gccggtggtg
      361 acgttctcgc agcgcatgct gtcgccccac tcgttggtct ggcattcgaa cggcccgacc
      421 atgttgaccg tcccggcggc cacatccggg ccgctgttca gggtcgtgtc accggcacag
      481 accagctcgg ccttgccgtc ggccacggtc aggccgccac cgtaatcaag ctggcacttc
      541 gggtcccgct tgaccttcca ggtcttgtgc ttgatgtcgc agcggaccat ggtctggtcg
      601 gcgatgcagc cgatgttgcc gtccggcgag gtgaaagtct cgagactgac cgacacgctc
      661 tcctgcttgt cggtcccgca gccggcaagg agcagtccgg cgaccaggac gctactcagg
      721 gtcagcttca gattccggct catccggttc ggcctcttcg gttgtttcgg tctcatcaac
      781 ttcatcaacc tcctggactt cttcgacttc cccggttgct tcaccttccg cggatgtatc
      841 cgtggtctcg gtgctttcga cctccgggtt ttcggcctgg tccttcttct tgttcttctt
      901 gcggcgccgt ttcttgttgc ccttggggaa gttccggagc agctcgcggg cgacggccga
      961 aggcttccgg gccattctca tgcgggcgcc gcgcagcacc tcttcgcctt ccggggtgtg
     1021 ggcgacggcg gcagccagca gcgaaaccgc gaggcggcgg tcctgcttgc gggcggcctg
     1081 gaccagcttg cgggcgttct tgccgtgggc gcggccggcc gaattgacca gcagtccgag
     1141 caggcgcttg gtttccggcc agcgctggac cgcgtactcc tcatggagct cgcgggtgta
     1201 ggaggcgagc cgccagaccc aggcctgggc ctggtcgcgg cgaccgatcc gcttgtcgac
     1261 cagcgcctgc agcagcacct tggtgccggc ccgcttctcg tcccttgacc aggtgccgac
     1321 gatgtcgatc gcgtcatcga acgcctcggg atccttcagg tttgcgatct cgctcagggc
     1381 cttgacccgg agctgggcgt tgcggttgcg ctggacgcag gtcagcaggt agcggcggcg
     1441 caattccttg gtccggtcat gctggagcat cgccttggcc cggcgccagc cctgggaagt
     1501 gacccgggcc ccggccaggg cagcccagac atgctgctcg gagtggttca ggttctcgac
     1561 tgcgatccgc cagatgtccc gttccagcac ctcgatccgc ttctgcggat catcggagac
     1621 cgggacgatg cggcgctcga cccggacctt gcggccgcgg ccacgacgga ccgggccgac
     1681 cacgatcgcg ccgccgcggt agacatcgcg gtcgagcagg ctgtgcaggg tgacgatcga
     1741 ctcgtcgtgg gtggtcgccg gatggacgaa gtcgatcacc agcccggctt ccttgttcgg
     1801 cgagcggcgg gtcacacgac cgacccgctg ctggtagatc cgcttcgagg cggtcggggc
     1861 caggtgcatg cagatggtcg cccggggcga gttccagccc tcggccagca gctgcgcatt
     1921 gcagagcacg tcgacttcgc cgcgttcgaa ggcggccagc gtgtccgaga gttcgcgctt
     1981 cggggtctcg ccggagaccg ccttggcgtt gattccaaac tcccgg
//
LOCUS       DAOQFI010000377         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_33643_length_2024_cov_3.906552, whole genome shotgun sequence.
ACCESSION   DAOQFI010000377 DAOQFI010000000
VERSION     DAOQFI010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33643_length_2024_cov_3.906552"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLE93_07030"
     CDS             complement(<1..481)
                     /locus_tag="PLE93_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNH86837.1"
                     /translation="MRPSMLEDVLDIPNHIRDALWRVDSAQLKARPSAGFVVCGMGGS
                     AIGAELARGVLDNRLTGPMVVSRSYGLPKWVTADWAVLASSYSGDTEETISSFKDAGE
                     AGTHRWVAGTGGELGARARDAGIGVVGLPGFFQPRMTVGYMTVVATYIAHLAGVAPDI
                     "
     gene            complement(462..1442)
                     /locus_tag="PLE93_07035"
     CDS             complement(462..1442)
                     /locus_tag="PLE93_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNH86838.1"
                     /translation="MQALVLVGGKGTRLRPLTYETPKPALTLVDRPFLNYMTEWLGSH
                     GVDEVILACGFLPDELKKVLGEGEPGGPKLRYLVEEETLGTAGAIRFALDHLDERFFA
                     LNGDVLADLDLTALWNQHEETGAQATLGLYEVEDSSSYGLVDIDSDGQVLKFSEKDPT
                     RTGTGLINAGTYVLEREAIAAIEPGREVSIETEFYPSITGKGLYARPLEGYWMDFGTR
                     QRYFDASWDILEGRIHTEVEKHSDGVFIAAGAEVDPTATIGPRAVIGAGAKVASGARV
                     TGSVVLEGSEIGADAVIENSIIGPGVNVAARGTVGNEVLGKNEIGETVNA"
     gene            complement(1449..>2024)
                     /locus_tag="PLE93_07040"
     CDS             complement(1449..>2024)
                     /locus_tag="PLE93_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNH86839.1"
                     /translation="PHFLRPGIGFGGSCFPKDVNALKLLAGNSGYHFQLLNSVIEVNE
                     LQKRRVVSKLTKHLGPLVGKRIALLGLAFKPDTDDMREASSLVLSARLRGEGAEVVAY
                     DPVTMSNARSLLPGVEMSDTAQEALAGSDAAVLVTEWSEFKEIDWTEAADRMRRPLLI
                     DGRNFLDAGQLRSAGFLYEGIGKAGTGDESG"
BASE COUNT          359 a          649 c          617 g          399 t
ORIGIN      
        1 ggatgtcggg ggccacgccg gccagatggg cgatgtaggt ggcgacgacg gtcatgtatc
       61 cgaccgtcat ccggggctgg aagaagccgg gcaggccgac cacgccgatg ccggcgtcac
      121 gggcccgggc gcccagttcg ccgccggtgc ctgcaaccca gcgatgggtg cccgcttcac
      181 cggcgtcctt gaacgaggag atcgtttctt ccgtgtcacc cgagtaactc gaagcgagca
      241 cggcccagtc ggcggttacc catttgggca gaccgtaaga gcgcgaaacg accatcgggc
      301 cggtcaggcg attgtcgagg accccgcggg caagctcggc gccaatcgcc gatccgccca
      361 tcccgcagac cacgaagccg gctgagggtc gagccttgag ctgtgccgaa tcaacccgcc
      421 agagggcatc acgaatgtgg ttggggatgt caagtacgtc ctcaagcatt gacggtctca
      481 ccgatttcat tcttgccaag gacttcattt cctaccgtac cccttgccgc aacatttaca
      541 ccaggtccga tgatcgagtt ttcgatcact gcgtcggcac cgatttcgct tccttcgagg
      601 acgaccgagc cggtcacacg ggcaccggaa gccactttgg cgcctgctcc tataaccgcc
      661 ctggggccga tagttgcggt tggatcgacc tcggcgccgg cggcgatgaa gaccccgtcg
      721 ctgtgctttt caacctcggt gtggatgcgg ccttcgagga tgtcccagct ggcgtcgaag
      781 tagcgctgcc tggtcccgaa gtccatccag tagccctcca gcggccgggc gtagagaccc
      841 ttgccggtga tcgaagggta gaactcggtc tcgatcgaga cctcccggcc cggttcgatc
      901 gcggcgatcg cctcccgctc caggacatag gtgccggcgt tgatcagccc ggtgccggtt
      961 cgcgtgggat ccttctcgct gaacttcagc acctggccgt cggagtcgat gtcaaccagg
     1021 ccgtagctgc tcgaatcctc gacctcgtag agccccagcg tcgcctgggc gccggtctct
     1081 tcgtgctggt tccagagggc ggtcaggtcg agatcggcaa gcacgtcgcc gttgagggcg
     1141 aagaaacgct cgtcaaggtg gtcgagggcg aaacggatcg cccctgcggt gccgagggtt
     1201 tcctcctcga ccagatacct gagcttcggc ccgcccggtt ctccttcacc gaggaccttc
     1261 ttcaattcat ccggcaagaa gccgcaggca aggatgactt cgtcgacccc gtggctgccc
     1321 agccactcgg tcatgtagtt cagaaacggc cggtcgacca gggtcagggc cggcttcggc
     1381 gtctcgtaag tcaggggtcg gagtcgggtt cccttaccgc caaccagtac caatgcctgc
     1441 aatttcgttt acccgctttc gtcaccggtc ccagccttgc cgattccttc gtaaaggaac
     1501 ccggcggagc ggagttggcc ggcatcaagg aagtttcgtc cgtctatgag caatggcctc
     1561 cgcatccgat cggccgcctc agtccaatcg atctccttga attccgacca ctcggtaacc
     1621 agtaccgcgg cgtccgaacc tgcaagtgcc tcctgggctg tgtcactcat ttcgaccccc
     1681 ggcaggagac tccttgcatt gctcatcgtt acggggtcat atgcaacaac ttccgcacct
     1741 tcgcctctca ggcgagcgga caaaacgagg ctcgatgctt ccctcatgtc gtcggtatcg
     1801 ggtttgaaag cgaggccgag aagggcgatc cgcttgccga ccagcggccc caggtgtttc
     1861 gtcagtttgc tcacgacccg tcgtttctgg agttcgttga cttcgatcac cgagttgagc
     1921 agctggaagt ggtagcccga gttaccggcc agtaatttca gcgcgttcac gtccttgggg
     1981 aagcaggaac cgccgaatcc gatcccggga cgcaggaagt gggg
//
LOCUS       DAOQFI010000378         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_33674_length_2023_cov_3.673272, whole genome shotgun sequence.
ACCESSION   DAOQFI010000378 DAOQFI010000000
VERSION     DAOQFI010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33674_length_2023_cov_3.673272"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..540
                     /locus_tag="PLE93_07045"
     CDS             148..540
                     /locus_tag="PLE93_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86840.1"
                     /translation="MNPAALDGSKGRLKPLFNTETFPGAYGALLPDLTGYDSPWVSFP
                     DLYAAKCQKADGASWLNVHDVSESDTRPRVGETLGRTWGTHLAEVNLTGGNLASVVVT
                     QEKAYLKKLGQARKAGAKKISKQREGHR"
     gene            625..1980
                     /locus_tag="PLE93_07050"
     CDS             625..1980
                     /locus_tag="PLE93_07050"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNH86841.1"
                     /translation="MSRVAGRTWLAVAFLAASFAFIAAVMPSFAKAEATVWICKPGLA
                     DDLCAGSISTKAGNPAQTIGFDRPDNPPVDCFYVYPTVSAQETPNANLDKDPEVKRVV
                     VQQARMFSRVCDVYAPMYRQDTSPGVYTERTEVAYQSALSGWKDYLQHYNKGRGVILI
                     GHSQGSATLARLIDEEIDPDPKLRKKLVGAILPGGNIYVKKGQLAGGMYENVPACSEV
                     GEYGCLVAYSMFNGTPADNPPFGDVGSGYWAYDMPRPSREQYEVVCTNPAELSGKTLL
                     TSLVNMDYLLEPPAGGVETANWNAYPDAVTASCDHEGTKHWLNVAFTEDADPFIKGMI
                     PIVASGNNWHVPEVNVAENNLVEIAQLQTDSYTAEQNRIAKLQAKIKSLQKQLAKAQR
                     NAGLQQKKANKFARQARKAKGKQKEALNRKAGSARRKAASERKRARNLKQRIAALKKQ
                     I"
BASE COUNT          404 a          662 c          642 g          315 t
ORIGIN      
        1 tcggagacat cgtgaacgtt cagccagctg gcaccatcgg ccttctcgag tttcctggag
       61 gatccgccgc cggccgattc cctctttggc agggtcggag gggccctctc ggagaacgtc
      121 gacccggcca cccacgaggt gatgtgcgtg aacccggccg cgctcgacgg gtcgaagggc
      181 aggctcaaac ccctgttcaa caccgagacc ttccccggcg cctacggggc attgctgccc
      241 gacctgaccg gctacgacag cccgtgggtc agtttccccg acctctacgc ggcgaagtgc
      301 cagaaggccg atggtgccag ctggctgaac gttcacgatg tctccgagtc agacacccgc
      361 ccccgggtcg gggagaccct gggccggacc tggggcacgc acctggcgga ggtgaacctg
      421 accggcggca atctggccag tgtcgttgtg acccaggaga aggcctacct gaagaagctc
      481 gggcaggccc gcaaggccgg ggcgaagaag atttcgaaac agagagaggg tcatcgctag
      541 cggccggacc gaaccggccg gcctgcagcg gtgttatcga tgatgcatag tccagaagtc
      601 aatttccccg ggaggagtct gatcgtgtcg agagttgctg gtcgtacctg gcttgccgtg
      661 gcctttttgg ccgcgtcatt cgccttcatt gccgcagtga tgccgtcttt cgcgaaagcc
      721 gaagccacag tctggatctg caagcccggt ctggccgatg atctttgcgc cggctccatc
      781 tcgaccaagg ccggcaatcc ggcccagacg atcggcttcg atcgtcccga caatcctccg
      841 gtggactgct tctacgtcta cccgacggtc agcgcgcagg agactcccaa cgcgaacctg
      901 gacaaggacc ccgaagtcaa gcgggtcgtg gtgcagcagg cccgcatgtt ctcccgggtc
      961 tgcgacgtct acgcgccgat gtaccggcag gacacttcgc cgggcgtcta caccgagcgg
     1021 acagaggtcg cctaccagag cgctctttcc ggctggaagg attacctcca gcactacaac
     1081 aagggccgcg gcgtgatcct gatcggccac tcgcagggct cggcgaccct ggctcgcctg
     1141 atcgacgagg agatcgaccc cgatccgaag ctgcgcaaga agctggtcgg agcgatcctg
     1201 cccggcggca acatctacgt gaagaagggc cagctggccg gtggcatgta cgagaacgtg
     1261 ccggcctgct cggaagtcgg cgagtacggc tgcctggtcg cctactcgat gttcaacggc
     1321 accccggccg acaacccgcc tttcggcgat gtcggctccg gctactgggc ctatgacatg
     1381 ccccggcctt cccgcgagca gtacgaggtg gtctgcacga atccggctga attgagcggc
     1441 aagaccctgc tcacctcgct ggtcaacatg gactacctgc tcgagccgcc ggccggtggc
     1501 gtcgagacgg ccaactggaa cgcctacccg gatgcggtca ccgcctcctg tgaccacgaa
     1561 ggcaccaaac actggctgaa cgtcgccttc accgaagacg ccgatccctt catcaaggga
     1621 atgatcccga tcgtcgccag cggcaacaac tggcatgtgc cggaagtcaa cgtggccgag
     1681 aacaacctgg tcgaaatcgc ccagctccag accgacagct acacggccga gcagaaccgg
     1741 atcgcgaagc tacaggccaa gatcaagtcg ctccagaagc agctggccaa ggcccagagg
     1801 aacgccgggc ttcagcagaa gaaggcgaac aagttcgcca ggcaggcccg caaggcgaag
     1861 ggcaagcaga aagaggccct gaaccgcaag gccggctcag cccgccggaa ggcggcttcc
     1921 gagcgcaagc gggcgaggaa cctcaagcag cggatcgccg ctctgaagaa gcagatctag
     1981 gcggattcgg cgggatcggc cggtttgccc gaggcgaacc gtc
//
LOCUS       DAOQFI010000379         2019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_33785_length_2019_cov_4.442464, whole genome shotgun sequence.
ACCESSION   DAOQFI010000379 DAOQFI010000000
VERSION     DAOQFI010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33785_length_2019_cov_4.442464"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLE93_07055"
     CDS             <1..267
                     /locus_tag="PLE93_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH86842.1"
                     /translation="SRVCGPVVDVQAQVAEETGDDVEFIHSEIYNDNDPNKGVNRQVQ
                     AFRLPTEPWLFAIDEDGIVRKRIEGAFGLGELREAVQSIRSESQ"
     gene            297..1220
                     /locus_tag="PLE93_07060"
     CDS             297..1220
                     /locus_tag="PLE93_07060"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNH86843.1"
                     /translation="MPARISAPIVFSALCLLGTLTVALSAVALIGTFISGDAPWAPVQ
                     ACCEAAIAKVSFGWLLLVAVGLISIVALVRLAFSSWLTIKANARVHSLRKLASTEKVA
                     GHDCLVLEDARPLAFCGGLVRPRVFLSRNLINELEPAELAAVVAHEAHHQNRRDPLRN
                     SITRVMAYGFFFLPMLREFGQKCVERSEIAADQAAVAGGESERKALASALLSLHGRES
                     EGVAVSLSSDRIGVLAGEKPSWIPSRNGALLSMSALGVLLAGPVLAAELVTRTPVGIE
                     ALGLHVCLAALIVAPLLLSAGFAWTRPQVRI"
     gene            1220..1609
                     /locus_tag="PLE93_07065"
     CDS             1220..1609
                     /locus_tag="PLE93_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNH86844.1"
                     /translation="MTNAENPPALSDLEREVMEQVWSASEEVSVRDVLNGINEANEKQ
                     RAYTTLMTILSRLEAKGMVTKRRKSKSDLFTPVLSRSDYLESRAKVEVDALVGEYGEI
                     ARALFVREIEDTDPDAAKKLKKLIDKS"
     gene            1646..>2019
                     /locus_tag="PLE93_07070"
     CDS             1646..>2019
                     /locus_tag="PLE93_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNH86845.1"
                     /translation="MSDNVKLSIALVLVGALGLLVYLSLGNDTPEGSANPEVTRADSH
                     YLTKAPDDKVTIVEFLDFECEACKAQFPTMERIREEYDGRINLAIRYFPLGGHQNGMN
                     AALAVEAASKQGALEDMYIKMY"
BASE COUNT          397 a          626 c          598 g          398 t
ORIGIN      
        1 agccgcgtct gcggtccggt ggtggacgtc caggcccagg tcgccgagga aaccggcgat
       61 gacgtggagt tcattcactc agagatctac aacgacaacg accccaacaa gggtgtcaac
      121 cggcaggttc aggcattccg attgcccacc gagccctggc tcttcgccat cgatgaggac
      181 gggatcgtcc ggaagcggat cgagggcgcc ttcggtctgg gtgagcttcg cgaagcagtc
      241 caatccattc gatcggaaag ccagtgatcc ggcctgccgt cggtaacctc tctcgagtgc
      301 ctgcgcgcat ttccgcacca atcgtcttct cggccctttg tctgctgggc acgctgaccg
      361 tggccctttc ggcggtcgcg ctgatcggaa ccttcatcag tggtgacgcc ccgtgggctc
      421 ccgttcaggc ttgctgtgaa gcagccatcg caaaggtttc gttcgggtgg ctcctcttgg
      481 tggcggttgg actgatctcg attgtcgcgt tggttcgcct ggccttcagc agttggctga
      541 cgatcaaggc caacgcgcgt gttcactccc ttagaaagct ggcttcgaca gagaaggtcg
      601 cgggccacga ttgcctggtt ctggaggacg ctcgaccgct tgcgttctgc ggcgggctgg
      661 ttcgcccgcg ggtcttcctc agccgaaacc tcatcaacga actagagccg gcagagcttg
      721 ctgccgtcgt tgctcatgag gcccatcatc agaaccggcg cgatcccctg cggaactcga
      781 tcacacgcgt catggcatac gggttcttct tcctgccaat gcttcgagag ttcggtcaga
      841 agtgtgtcga acgctccgaa attgccgccg accaggctgc ggtagccggc ggtgagagcg
      901 aacgaaaggc acttgcgagc gccctcctct cgctgcatgg acgcgaaagc gagggggtcg
      961 cagtgtcgct gtcatccgat cggatcggag ttcttgcggg tgagaagcct tcctggattc
     1021 ccagtagaaa cggggccctc ctgagcatgt ccgctctggg ggtcttgctg gccggcccgg
     1081 tactggcagc cgaactcgta actcgcacac ccgtcggcat cgaggctttg ggtcttcacg
     1141 tttgcctcgc cgcgctcatc gtcgctcctc ttctgctttc ggcgggattt gcctggaccc
     1201 gtccccaggt gcggatctga tgaccaacgc tgagaaccca cctgccctct cggacctcga
     1261 acgtgaagtc atggaacagg tctggtcggc atcggaagaa gtgtcggtac gagacgtcct
     1321 gaacgggatc aacgaagcga acgagaagca gcgggcctac acaaccctga tgaccatcct
     1381 ttcccgcctt gaagcaaagg gaatggtcac caagcgcagg aagtcgaagt ccgacctctt
     1441 caccccggtg ctctcccgaa gcgactacct cgagtcccga gcaaaggtcg aagtcgacgc
     1501 actggtcggc gagtacggcg agatcgcccg tgccttgttc gtccgtgaga tcgaagacac
     1561 cgatcccgac gcagccaaga aactcaaaaa gctgatcgac aagtcctaat tactacagct
     1621 tgtagtagca tcggctgcca agtcgatgtc ggacaacgtc aaactctcaa tcgccctcgt
     1681 gctcgtgggc gcactcggac ttctggtcta cctcagtctc ggaaacgaca ctccggaagg
     1741 atccgcaaat cccgaggtca ctcgcgcgga ctctcactac ctgaccaagg cgccggacga
     1801 caaggtcacg atcgtagagt tcctcgactt cgaatgcgaa gcctgcaagg cccagttccc
     1861 gacgatggag cgaatccgcg aggagtacga cggcaggatc aacctagcta tccggtactt
     1921 cccactcggc ggtcatcaga acggaatgaa cgccgccctc gcggtggagg cggcctccaa
     1981 gcagggagcc ctcgaggaca tgtacatcaa gatgtacga
//
LOCUS       DAOQFI010000380         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_33960_length_2014_cov_4.022460, whole genome shotgun sequence.
ACCESSION   DAOQFI010000380 DAOQFI010000000
VERSION     DAOQFI010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33960_length_2014_cov_4.022460"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="PLE93_07075"
     CDS             <1..1444
                     /locus_tag="PLE93_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86846.1"
                     /translation="PTAPLVWSHARTVSAAVLGNTCTTSFPLELKTTGTLVEPGGNHD
                     AAPFDPDTGEGSVYSSFTVPNSTGGALCGVVDGQIGGAGDIWLSGTGGIDEGIVTVYP
                     GDDGTSLSTTDGGWVHSTSYGPLCALIPGVLCPAMNGTFESANGSSGAADGFLRDTAT
                     GLTVTPTGLGQTSTGVWTSPEFTYDGAEGEPAETLSFALARKGTLSDLLSQSSANWQV
                     DLVKSSDSSIIPLIPNGPATNADSWVNIPGSALAADGLTIGDAYRIVITTNVITETLG
                     LAPSGTFDYDEVALTARRATPTGPTGPTGPTEPTGPTGPTEPTGPTGPTEPTGPTGPT
                     SPTGPTGPTGPTGPNKPVTLTKGNAAFNGKRLFIRLYCPREFKPKCNTVSVPVTRKVA
                     NKAPKKIKRLSKPMARPIRVTVKSGKWKKVSYLIKPAYRNKVRKMAGKNKRLLAIRDY
                     IKAKKFAGRKFKGKTRTVFHSHKVVIGKAR"
     gene            complement(1532..>2014)
                     /locus_tag="PLE93_07080"
     CDS             complement(1532..>2014)
                     /locus_tag="PLE93_07080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNH86847.1"
                     /translation="PAFKEDGKITAGNSSQVSDGAAAVLLMTKEKADELGLKARARIV
                     DQTAVGVDPVIMLTGPIPATRKILERNDMTMDDIDLTEINEAFASVVKAWEIELQPDM
                     DKVNVNGGAIALGHPLGSTGARLMTTLLHELERQDKKTGLVTMCCGGGIGTATLIERI
                     "
BASE COUNT          374 a          715 c          604 g          321 t
ORIGIN      
        1 gcccaccgcc ccgctcgtct ggtcccacgc ccgcaccgtc agcgctgccg tactcggcaa
       61 tacctgcacg acctccttcc cgctcgaact caagaccacg ggcactctgg tcgaacccgg
      121 cggtaaccat gacgcggccc cctttgaccc cgacaccggt gaggggagcg tctacagctc
      181 gttcacggtg ccgaactcga ccggtggcgc cctctgcggc gtggtcgacg gccagatcgg
      241 cggcgccggt gacatctggc tctccggcac tggcggaatc gatgaaggca tcgtcaccgt
      301 ctatcccggc gatgacggca ccagcctctc caccaccgac ggcggctggg tccacagcac
      361 ctcgtacggc ccgctctgcg cactcatccc gggagttctc tgccccgcga tgaacgggac
      421 cttcgaatca gccaacggca gctccggggc cgccgacggc ttcctgcgcg acacggccac
      481 cggcctgaca gtcaccccga ccggcctcgg ccagacctcg accggagtct ggaccagccc
      541 cgagttcacc tatgacgggg ccgaaggcga accggccgag actctgagtt ttgccctggc
      601 caggaaaggc accctctccg acctgctcag tcagtccagc gccaactggc aggtcgacct
      661 ggtcaagtcc agcgacagtt cgatcatccc gctgattccg aacggtcccg caaccaatgc
      721 cgattcctgg gtgaacatcc cgggcagcgc gctggcggct gacggcctga cgattggcga
      781 cgcgtatcgc atcgtgatca ccaccaacgt gatcaccgag acgctcggcc tggccccctc
      841 tggaaccttc gactacgacg aggtggcgct cacggcccgt cgcgcaaccc cgaccggtcc
      901 gacgggcccc actggcccga ccgaacccac aggtcctact ggcccgacgg aaccgaccgg
      961 tcctaccggc cccaccgagc caaccggccc gaccggtccg acttccccga ccggccccac
     1021 cggccccacg ggaccgaccg gaccgaacaa gccggtcacc ctgaccaagg gcaacgccgc
     1081 cttcaacggc aagcgactgt tcattcgcct gtattgccca cgcgagttca agccgaagtg
     1141 caacacggtt tcggtgccgg tgaccaggaa ggtcgcaaat aaggccccga agaagatcaa
     1201 gcgcctgtcg aagccgatgg ccaggccgat ccgggtgacg gtcaagtcgg gcaagtggaa
     1261 gaaggtctcc tacctgatca agcctgccta ccggaacaag gtcaggaaga tggccggcaa
     1321 gaacaaacgg ctgctggcga tccgcgacta catcaaggcg aagaagttcg ccggccggaa
     1381 gttcaagggc aagacccgaa cggtcttcca cagccacaag gtcgtgatcg gcaaggccag
     1441 ataacgaacc tccctcccaa atgaagtgcc ctccaaatga aagggcccgg gtctctggac
     1501 aagacccggg ccctttcgcc ttctgacgga ctcagattcg ttcgatgagc gtggcggttc
     1561 ctatcccgcc gccgcagcac atggtgacca gaccggtctt cttgtcctga cgctcgagct
     1621 catggagcag ggtggtcatc aggcgggcgc cggtcgagcc gagcgggtgg ccgagggcga
     1681 tcgcgccgcc gttgacattt accttgtcca tgtcgggctg gagctcgatc tcccaggcct
     1741 tgaccaccga ggcgaaggcc tcattgatct cggtcaggtc gatgtcatcc atcgtcatgt
     1801 cgttgcgctc gaggatcttg cgggttgccg ggatcggccc ggtcagcatg atcaccgggt
     1861 cgacaccgac cgcggtctga tcgacgatcc gggcgcgggc cttgagcccg agctcgtctg
     1921 ccttctcctt cgtcatcagc agaaccgcgg cagcgccgtc ggagacctgt gaggagttgc
     1981 cggcggtgat cttgccgtct tccttgaagg cggg
//
LOCUS       DAOQFI010000381         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34203_length_2006_cov_3.307022, whole genome shotgun sequence.
ACCESSION   DAOQFI010000381 DAOQFI010000000
VERSION     DAOQFI010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34203_length_2006_cov_3.307022"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..651)
                     /locus_tag="PLE93_07085"
     CDS             complement(109..651)
                     /locus_tag="PLE93_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86848.1"
                     /translation="MNTRNHSTPEMINRPPENRPGETRLLDGGFRAGLALFTCLFAFW
                     FMFAADDAGAAAYKGKTKGGSSITFALKGNRVTGIRTVVPTLCLETTGGYTSRAGSEL
                     FQPVAAGRIGRTVKSKALQPAAMNVGAKATKNYEVTVNRRGRTVSGKLKLNFSFLIPD
                     LYGARIYICSGVTTFSATPR"
     gene            complement(648..>2006)
                     /locus_tag="PLE93_07090"
     CDS             complement(648..>2006)
                     /locus_tag="PLE93_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86849.1"
                     /translation="PDAQAANVSFVGDDGNVSRTSPDGTITEQVTTDATAETRYVTPT
                     QKNDGTIVAIKRVSSNAFAYFLNPENGQVKDTWLLPKTGAGSFAPFNGGAISPDGGMF
                     VYDWHYFDCWTNPCTLNQKVSFISGPGTTNPCLVNCHTYYIRPRWIPGTPYAGFVDTD
                     FRRIWVQKAGSAEPNAWLGFNDPNAGDMHSFDVSSNGLTVLEVSPEGSEAAEFSFWNT
                     GGTPPAGTPSHRCSITAIAQTPAYPRFSPDGSMITWQDRGSIYVAPVPSTTGGGTCNL
                     NPVKVGEGMEPSFGLATLKRDEPTDPTDPTDPTDPTDPTDPTDPTDPTDPTDPSDPSD
                     PSDPTDPGPGTPKVTLKVNPGTAKVKAGRPVTLTAAVTASGANAPTVKVCATVSGKAR
                     NVIKPAACRTLRNVARGSTVRTSLVIRTAKKARGSYPVRVAASGPGLTPVNSNNKVQV
                     TK"
BASE COUNT          290 a          599 c          756 g          361 t
ORIGIN      
        1 aggtcgatca cgttgccgcg agtgatgaac tcgcggtaac cctgaatcat cttcacgggc
       61 cgaaactacc cggcggcccc gacgcaaagc cggggccgcc cggccggttc agcggggagt
      121 ggcgctgaag gttgtgactc cggagcagat gtagatccgg gccccgtaga ggtcggggat
      181 caggaaggag aagttgagct tcaacttgcc cgagacggtg cggccgcgcc tgttcaccgt
      241 gacctcgtag ttcttggtcg ccttggcgcc aacgttcatc gcggccggct ggagcgcctt
      301 gctcttcacc gtccggccga tccgccccgc cgcgaccggc tggaacagct cgctgccggc
      361 ccgggaggtg tatccgcccg tggtctccag gcagagggtc gggaccacgg tgcggatgcc
      421 ggtcacccgg ttgcccttga gggcgaaggt gatcgagctg ccgcccttgg tcttgccctt
      481 gtaagccgcg gcgccggcat cgtcggccgc gaacatgaac cagaaggcga acagacaggt
      541 gaagagggcc aggccggcac ggaaaccgcc gtcgagcagg cgggtttcac cggggcggtt
      601 ttccggcggg cgattgatca tttcgggggt gctgtggttt cgggtgttca cttggtcacc
      661 tggaccttgt tgttggagtt gactggggtg aggccggggc cggaagcggc aacccggacc
      721 gggtagctgc cacgggcctt cttcgcggtg cggatgacca gggaagtcct gacggtgctg
      781 cccctggcga cgttgcggag cgtcctgcag gcggccggct tgatcacgtt gcgggccttg
      841 ccgctgaccg tggcgcagac cttgacggtc ggcgcgttcg ccccgctggc ggtaaccgcc
      901 gcggtcaggg tcaccggtcg gccggccttg accttcgccg tacccgggtt caccttgagc
      961 gtgaccttcg gcgtgccggg cccggggtcg gtcgggtcag acgggtcgga agggtcgctc
     1021 ggatcggtcg ggtccgtggg gtccgtggga tccgtgggat cggtcgggtc ggtcgggtcc
     1081 gtggggtcgg tcggatcggt cgggtcggtc ggttcgtccc gcttgagggt ggccagcccg
     1141 aaggaaggct ccatgccctc gccgaccttg accgggttca ggttgcaggt tccgccaccg
     1201 gtggtcgaag gaaccggggc gacgtagatc gagccacggt cctgccaggt gatcatcgag
     1261 ccgtccggcg agaagcgcgg gtaggccggg gtctgggcga tcgccgtgat cgagcaacgg
     1321 tgcgaagggg tcccggccgg cggggtgccg ccggtgttcc agaaggagaa ctcggctgcc
     1381 tcggatccct ccggagagac ctccagcacg gtgaggccgt tcgaggagac gtcgaaggaa
     1441 tgcatgtccc ctgcgttggg gtcgttgaag ccgagccagg cgttcggttc ggcgctgccc
     1501 gccttctgga cccagatccg gcggaagtcc gtgtccacga aaccggcgta gggcgtaccc
     1561 gggatccagc gcggccggat gtaataggtg tggcagttga ccaggcaggg gttggtcgtg
     1621 cccggaccgc tgatgaacga aaccttctga ttgagggtgc aggggttggt ccagcagtcg
     1681 aagtaatgcc agtcgtagac gaacatgccg ccgtccggcg agatcgcacc gccgttgaac
     1741 ggggcgaagg aaccggctcc ggtcttcggc agcagccagg tgtccttgac ctgcccgttc
     1801 tccggattca ggaagtaggc gaacgcgttc gagctgaccc gcttgatcgc cacgatcgtg
     1861 ccgtcgttct tctgggtcgg agtgacgtag cgcgtctcgg cggtggcatc cgtggtcacc
     1921 tgctcggtga tcgtcccgtc gggcgaggtg cgggagacgt tgccgtcgtc gccgacgaag
     1981 gagacgttgg ccgcctgcgc gtcggg
//
LOCUS       DAOQFI010000382         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34234_length_2005_cov_3.364103, whole genome shotgun sequence.
ACCESSION   DAOQFI010000382 DAOQFI010000000
VERSION     DAOQFI010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34234_length_2005_cov_3.364103"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLE93_07095"
     CDS             complement(<1..614)
                     /locus_tag="PLE93_07095"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNH86850.1"
                     /translation="MTVSVVTGGAGFVGSHLCDALLEKGNKVICIDNLDTGSLQNIEH
                     ITNPDFTFLPQDITEEVHLDGEVDFVYHLASPASPIDYARLPLHTLKVGAQGTWHMLG
                     VAKFKRARFLLASTSEVYGDPQVHPQEESYWGHVNPIGPRGVYDEAKRYAEALTMAYH
                     RQQGVDTSIVRIFNTYGSRMRAHDGRAIPTFMRQALQNQPLTVFG"
     gene            701..1495
                     /locus_tag="PLE93_07100"
     CDS             701..1495
                     /locus_tag="PLE93_07100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH86851.1"
                     /translation="MSAVRRKAIGVLLLAGLVLFCLPAASGAVPAWQSAPVIPSLSNG
                     DQARLKDRVAAGERTGLRSGVFAKVGDSNTEFSPDLYGLACRKPTGLSAALRGTLATY
                     SRVRLPNARALPGCRPWTSFSRRSSAAQAGVHSTWPLTPVKDLPDESYWAKPPGCPLK
                     GTPLSCEIDATMPRYAMVMLGTNDIGMDIAFGIQPGSRIVSRLGGVVRALLARKVVPV
                     LSTIPPIIRPDGTRQGEVTAGVARSNAGIFELARKWHLPMINLWGP"
     gene            1507..1707
                     /locus_tag="PLE93_07105"
     CDS             1507..1707
                     /locus_tag="PLE93_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86852.1"
                     /translation="MIDLGLAGDGLHLSVAGAGGEMVGVQPGASTFADSVDFTAAGLR
                     YGANRRNLIWLKTLARLDRVTG"
     gene            1709..>2005
                     /locus_tag="PLE93_07110"
     CDS             1709..>2005
                     /locus_tag="PLE93_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86853.1"
                     /translation="MGRRFTAVLLAGLILLAGCGSSGEDSGQAPDPSRAPARAGESAR
                     TPQPVADLSSGSVLPDLSGAAGDRLRREVARADERKLNPDVFAKVGDSNTEWPQN"
BASE COUNT          297 a          676 c          685 g          347 t
ORIGIN      
        1 ccgaaaacgg tcagcggctg gttctgcagg gcctgccgca tgaaggtcgg gatcgcccgc
       61 ccgtcatggg cgcgcatccg tgagccgtag gtgttgaaga tccgcacgat cgaggtgtcg
      121 acgccctgct ggcggtggta ggccatggtc agggcttcgg cgtaccgctt cgcttcgtcg
      181 tagaccccgc gcgggccgat cgggttgacg tggccccagt agctctcttc ctgggggtgg
      241 acctgggggt cgccgtaaac ctcggaggtc gaggcgagca ggaagcgggc ccgcttgaac
      301 ttggccacgc cgagcatgtg ccaggtgccc tgggcgccga ccttcagggt gtgaagcggc
      361 agccgcgcat aatcgatcgg gctggccggg gaggccaggt ggtagacgaa gtcaacctcg
      421 ccgtcgaggt ggacttcctc ggtgatgtcc tgcggcagga aggtgaaatc gggattcgtg
      481 atgtgctcga tgttctggag tgatcctgtg tccaggttgt cgatgcagat caccttgttg
      541 cccttttcga gcagggcatc gcaaagatgg gatccgacaa aaccggctcc gccggtaact
      601 acgctgactg tcatgggcgg atcgtattca aggttcggcc gacgatgccg tcctgatcgt
      661 ctaccgttta ctgcctgtca cttcgggggg attggaagcc atgagcgccg tcaggcgaaa
      721 agcaatcggg gtcctgctgc ttgccggcct tgtcctcttc tgcctgccag ccgcctcggg
      781 agcggtcccg gcctggcagt cggccccggt tatcccctct ctttcgaacg gggatcaggc
      841 caggctgaag gaccgggtcg cggccggcga gcggaccggg ttgcgcagcg gcgtcttcgc
      901 caaggtcggg gattcgaaca ccgagttctc ccccgacctc tacggcctcg cctgccggaa
      961 gccgaccggt ctgagcgccg cccttcgcgg caccctcgcc acctacagcc gcgtgcgcct
     1021 gccgaacgcc agggcgctgc cgggctgccg gccgtggacc tcgttctccc gccgctcctc
     1081 ggccgcccag gccggcgtgc actcgacctg gcctctaacc ccggtcaagg atcttcccga
     1141 cgagagctac tgggccaagc cgcccggctg cccgctcaaa ggcactccgc tgagctgcga
     1201 gatcgacgcg accatgcccc gctacgccat ggtcatgctg ggcacgaatg acatcggcat
     1261 ggacatcgct ttcggcatcc agcctggcag caggatcgtc agccgcctcg gcggcgtggt
     1321 cagggccctg ctcgcccgga aagtggttcc cgtgctgagc acgatcccgc cgatcatccg
     1381 gccggacggc accaggcaag gcgaggtcac ggccggtgtg gcccggagca acgccggcat
     1441 cttcgaactc gcccggaagt ggcacctgcc gatgatcaat ctctgggggc cctgaaggag
     1501 ccttcgatga tcgacctcgg cctggccggg gacggcctcc acctcagcgt cgcgggggca
     1561 ggcggggaga tggtcggtgt ccagccggga gcttcgacct tcgccgactc ggttgatttc
     1621 actgcggccg gcctgcgcta cggggcaaac cgccgcaacc tgatctggct gaagaccctg
     1681 gcccggctcg accgggtcac cggttgacat gggccgccgc ttcacagccg tcctcctggc
     1741 cgggctgatc ctgctggccg gttgcggctc ctccggtgag gattccgggc aagctcccga
     1801 cccctcccgg gcacctgccc gggccgggga atcggcgcgg accccgcaac cggtggccga
     1861 cctttcgtcc ggctcggtgc tcccggacct ctcgggggcg gccggtgacc gcctgcgccg
     1921 ggaggttgcc cgggccgacg aacggaagct gaacccggat gtcttcgcca aggtcggcga
     1981 ttcgaacacc gagtggccgc agaac
//
LOCUS       DAOQFI010000383         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34240_length_2005_cov_2.541026, whole genome shotgun sequence.
ACCESSION   DAOQFI010000383 DAOQFI010000000
VERSION     DAOQFI010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34240_length_2005_cov_2.541026"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLE93_07115"
     CDS             complement(<1..831)
                     /locus_tag="PLE93_07115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH86854.1"
                     /translation="MEIVLPVLNEEKILETSVRRLDGFLREQFPIAARITIVDNASTD
                     RTESIGTRLADELDRVSYVRLERKGRGLALREAWSVTAAPIACYMDIDLSTDLNALFP
                     LVAPLLSGHSDLSIGTRLSPSSKVTRGPRREFISRTYNWILRSTLRARFTDAQCGFKA
                     IRTESVKPLLAEVKDDGWFFDTELLMLAQRRGLRIHEVPVDWTDDPDSRVDIIPTAWI
                     DLKGVARLLSAGQVSRFVIVGIASTIAYALIYLLLRQGIEAGPANAISLALTAVGNTA
                     A"
     gene            complement(966..>2005)
                     /locus_tag="PLE93_07120"
     CDS             complement(966..>2005)
                     /locus_tag="PLE93_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH86855.1"
                     /translation="SAVADQAGRITYVAVPTSDMKETLDQLLLIEAIVTAAVLIALAG
                     LAWWMIGIGLRPLERMSETAGEIAAGDLSRRVEDEDPRTETGRLGISLNKMLHQIEDS
                     VRQREESEARMRQFLADASHELRTPLASIRGYAELYRLGAIPQGAEADRAISRIENES
                     ARMGDLVDGLLTLARLGELPEPVRRPVDLAVVAAECTADAKAMSPDREFSLDTAEDVT
                     IRGNEDQIRQLLANLLRNAIDHTPEGTPVETTIASGGRTIEIEVRDHGPGIDEAHADQ
                     LFERFWREGESRSRKTGGAGIGLAVVAGIAGVHGGDASAANDPDGGAIFQVRLARDPD
                     SETSQAAPTGV"
BASE COUNT          303 a          650 c          711 g          341 t
ORIGIN      
        1 ggcggccgtg ttgcccaccg cggtgagcgc cagcgaaatc gcgttggcgg gccccgcttc
       61 gatcccctgc ctgagcagca ggtagatcag ggcgtaggcg atcgtcgagg cgatgccgac
      121 gatcacgaaa cgcgagacct gcccggcgct gagcagccgg gcaacccctt tgaggtcgat
      181 ccaagcggtc gggatgatgt ccaccctcga gtcgggatca tcggtccagt cgaccggaac
      241 ctcgtggatg cgaagaccgc ggcgctgggc cagcatcagc agttcggtgt cgaagaacca
      301 gccgtcgtcc ttcacctcgg cgagcagcgg cttcacgctc tcggtgcgga tcgccttgaa
      361 gccgcactgg gcgtcggtga accgggcccg aagggtggac cggaggatcc agttgtaggt
      421 gcgggagatg aactcgcggc gcgggccgcg agtgaccttc gaggaaggcg agagccgggt
      481 gccgatcgaa agatcggaat gtcccgaaag cagcggggcg accagcggga acagcgcgtt
      541 gaggtcggtc gagaggtcga tatccatgta acacgcgatc ggcgcggcgg tcacggacca
      601 ggcctcgcgg agggccagcc cgcgcccctt gcgttccagc cggacataag aaacccggtc
      661 gagctcgtcc gccagacggg tgccgatcga ttcggtgcgg tcggtcgagg cgttgtccac
      721 gatcgtgatc cgcgcggcga tcggaaactg ttccctcagg aagccgtcaa ggcggcgaac
      781 cgaggtttcg aggatcttct cctcattcaa tacgggcaga acgatctcga tgggctgatc
      841 gtacccgctg gcctccgggc cggccgcgtt cagggggaga gaggttgggc ccttcgtttc
      901 ggccggcccg gttgcctgga gagaggaggt tgcgggtgtc atggaactac tgtcggggac
      961 cggcctcaga cgccggtggg agctgcctga gaagtttctg agtccgggtc gcgggccagc
     1021 ctgacctgga agatcgcacc gccgtccggg tcgttggccg ccgaggcatc gccgccgtgg
     1081 actcccgcga tacctgccac cacggccagg ccgatcccgg ctccgccggt cttgcggctg
     1141 cgcgattcgc cttcgcgcca gaaccgctcg aagagctggt cggcgtgcgc ctcgtcaatc
     1201 cccgggccgt ggtcgcggac ctcgatctcg atcgtgcgtc ccccgctggc gatcgtggtc
     1261 tcgaccggcg tgccctccgg cgtgtggtcg atcgcgttgc ggagcaggtt ggcgagaagt
     1321 tgccggatct ggtcctcgtt gccgcgaatc gtcacgtcct cggcggtatc gaggctgaac
     1381 tcccggtccg gtgacatcgc cttcgcatca gcggtgcatt cggcggcgac cacggcgaga
     1441 tcgaccgggc ggcgcaccgg ttccggcagt tcgccgagcc gggcgagggt gaggagcccg
     1501 tcgaccaggt ctcccatccg ggccgattcg ttttcgatcc gcgagatcgc ccggtctgcc
     1561 tccgcgcctt gcggtatcgc gccgaggcga tagagctccg cgtagccgcg gatcgaggcg
     1621 aggggggtcc gcagctcatg cgaggcgtcg gcgaggaact ggcgcatccg tgcctcgctt
     1681 tcctcccgct ggcggaccga gtcctcgatc tggtggagca tcttgttgag agagatgccg
     1741 agccggccgg tttccgtcct cgggtcctcg tcctcgaccc ggcgggaaag gtcaccggcg
     1801 gcgatctctc ctgccgtctc gctcatccgc tccaggggcc tcagcccgat cccgatcatc
     1861 caccaggcga ggccggccag ggcgatcagc acggcggcgg tgacgatcgc ctcgatcagg
     1921 agcagctggt cgagcgtttc cttcatgtcg ctggtcggca ccgcaacata ggtgatccgt
     1981 cccgcctggt cggcgaccgc gctgg
//
LOCUS       DAOQFI010000384         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34382_length_2000_cov_3.008740, whole genome shotgun sequence.
ACCESSION   DAOQFI010000384 DAOQFI010000000
VERSION     DAOQFI010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34382_length_2000_cov_3.008740"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLE93_07125"
     CDS             <1..255
                     /locus_tag="PLE93_07125"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571304.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HNH86856.1"
                     /translation="GYPVIERDIARSELYLADEIFMCGTAAEVVPVREVDDHPLGDPG
                     EVTRLVQQGYEDAIYGRSPEYSEWLDLVGEPATKTEPSTV"
     gene            411..>2000
                     /gene="cimA"
                     /locus_tag="PLE93_07130"
     CDS             411..>2000
                     /gene="cimA"
                     /locus_tag="PLE93_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNH86857.1"
                     /translation="MTAVKLYDTTLRDGMQGQGMSLSAAEKVRVVHALDQLGVQFIEA
                     GFPASNPKEAELFELLAGEKLEQAAICAFGMTRRRGVEADQDEALQILAATFAPVITL
                     VGKTWALHLEKVTKVSREENLGMISESVAYLVGEGKRVMYDAEHFFDAWADDPDYSLE
                     CLKAARDAGAEVLVLCDTNGANLPSQIRPAVEAVKEALPEAELGIHCHNDLECGVANA
                     LAAVTAGARQVQGTINGYGERCGNSNLISILPALQLKLGYEVVPPGKMKQLSGVSHFV
                     ADLLNVPVDPDQAYVGRNAFAHKGGMHVAGIAADARTFEHMDPGAVGNSRKVLASELS
                     GKATVLARAEEIGLEFDDEAARKAVEELKQKEHDGYHYEAAEASFDLLLRREAGQYKP
                     LFTFESFKVLTELRAGEKVETEAIVKIKVGDERYVEVAEGNGPVNALDKALRQAITRH
                     YPELKKIELTGYRVRILDTAHGTGAGTRVLLDSKREEGGDSWGSIGVSPNIIEASWEA
                     LVDSLAYPFQNGNSAADSGEAG"
BASE COUNT          411 a          604 c          632 g          353 t
ORIGIN      
        1 ggataccccg tgatcgagcg ggacatcgcc cgttccgagc tttacctggc cgatgaaata
       61 ttcatgtgcg gcaccgccgc cgaagtggtt ccggtccggg aagtcgatga tcatccgctc
      121 ggtgatcccg gtgaagtgac ccggctcgtc cagcaaggct acgaagatgc gatctacggg
      181 cgctcacccg agtattcgga atggctggat ctggtcggcg agccggcaac caagaccgaa
      241 ccaagtacag tctgagatca atgcttcacg ccatcaccga aatcaggact acccgaatta
      301 cgggggctgc ggggcgctga ccctgcggca gcgcggcggg acgccgtgtg cagtcctccc
      361 ggacccgacc atggttccgg gcctcctgat ttcctgaaag gcagtttccg atgacagcag
      421 tgaagctcta tgacacgact ctccgcgacg gcatgcaggg tcagggcatg tccctgtccg
      481 ctgctgaaaa ggtgcgtgtg gttcatgccc tcgatcagct cggagttcag ttcatcgagg
      541 caggctttcc ggcttcaaac ccaaaagagg ccgagctctt cgagctgctc gccggggaaa
      601 aactcgagca ggccgcgatc tgcgccttcg ggatgacccg ccgacgcggg gtggaggctg
      661 accaggatga ggccctgcag atcctcgcgg cgactttcgc cccggtcatc accctggtcg
      721 gcaagacctg ggctcttcat ctggaaaaag tgaccaaggt ctcccgcgaa gaaaacctgg
      781 ggatgatcag tgaatcggtc gcctacctgg tcggagaggg caagcgagtc atgtacgacg
      841 ccgaacactt cttcgacgcc tgggccgatg accctgacta ttcactcgaa tgcctgaagg
      901 ctgcccgtga tgccggcgcc gaggtgctgg tgctatgcga caccaacggg gccaacctgc
      961 ccagccagat caggcccgcg gtcgaggctg tgaaggaggc tctgcccgag gccgaactcg
     1021 gtattcattg ccacaatgac ctcgaatgcg gagtcgccaa tgcactggcc gcggttaccg
     1081 caggggcgag gcaggtccag ggcacgatca acggctatgg agagcgctgc ggcaactcga
     1141 acctgatctc gatcctgccc gcacttcagc tcaagctggg gtatgaagtt gtgccgcccg
     1201 ggaagatgaa gcaactctca ggtgtctctc actttgtggc cgacttgctc aatgttccgg
     1261 tagatcccga ccaggcatat gtaggtcgca acgcctttgc gcacaaaggc ggcatgcatg
     1321 tcgccgggat cgcggccgat gcccggacct tcgagcacat ggatccgggc gcggtcggga
     1381 attcccgcaa ggttctcgcc tccgaacttt ccggcaaggc gactgtgctg gcccgggccg
     1441 aagaaatcgg acttgagttc gacgacgagg cagcccgcaa ggcggtcgag gaactgaaac
     1501 agaaggaaca cgacggctat cactacgaag cagcggaggc ttccttcgac ctgttgctac
     1561 gccgcgaggc cgggcagtac aagccgctct tcaccttcga gtccttcaag gtcctgacgg
     1621 aattgcgtgc cggcgagaag gtcgagaccg aggcgatagt gaaaatcaag gtcggtgatg
     1681 agcgctacgt ggaggtggcc gaaggaaatg gtccggtcaa cgcgctggac aaggctctac
     1741 gtcaggcgat cacgcgccac taccccgaac tgaagaagat cgaactgacc ggataccggg
     1801 taaggatcct cgacacggcc cacggtaccg gcgccggcac ccgcgtcttg ctcgattcga
     1861 agcgggaaga aggcggcgat tcctggggct cgatcggggt ctcgccgaac atcatcgagg
     1921 cttcctggga agccctggtc gactcgcttg cctacccctt ccagaacggc aactcggccg
     1981 cggattccgg ggaagccggt
//
LOCUS       DAOQFI010000385         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34387_length_2000_cov_2.779434, whole genome shotgun sequence.
ACCESSION   DAOQFI010000385 DAOQFI010000000
VERSION     DAOQFI010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34387_length_2000_cov_2.779434"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLE93_07135"
     CDS             complement(<1..307)
                     /locus_tag="PLE93_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86858.1"
                     /translation="MKLRSGPEGLMVEAGPGEWVSLPALKREATELDLDDGQTTDMVA
                     FLAAGEPARKAVQKSLDSVDADKVKADPDQAGLPLRPRSMRAFMLWESHMIASSRVLV
                     "
     gene            complement(304..1188)
                     /locus_tag="PLE93_07140"
     CDS             complement(304..1188)
                     /locus_tag="PLE93_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH86859.1"
                     /translation="MSAVSKIGHMAMRVSDLDRAVEFQSEVMGLVETERQGGTSFMTC
                     NDRHHELILIQSGKRGYDHIGLEVATAEDLEAVRAQAAAAGGDVLTQIQEGETGIDRC
                     FFVRSPAGHVFKIFLGMEKVAEPEPGDRPSQFEHVSTKALRMGREERFLSEGLGFRFS
                     DRLGRTASWWHCDPDHHGMAVVIAPRAELSHYAWSLEDLNALGRTADRLRARGQRAIW
                     GPSRHGPGNNLFLYFHDADGAMIECCAELEKMPPEGDYKPRKWTGGMKDINQWGSTPP
                     AKFLLTGFPINHREGEVK"
     gene            complement(1185..>2000)
                     /locus_tag="PLE93_07145"
     CDS             complement(1185..>2000)
                     /locus_tag="PLE93_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNH86860.1"
                     /translation="GLTSAIHDVHNLTWKIASVLKGEAGESLLETYEPERRPVVARNA
                     QRSLENAINHLGTMAEIGVTPENSTEENWAAMKRLWNEDPADQEFREKVARGARSQSM
                     EFNELNVEYGYAYDSAAVVADGSPPAPNPDEVRIYLPSTRPGSPLPHAWLDDLEGNRQ
                     PIKDLVRPGRFLLIAGEEGDEWLQAANDLAGSAKVPLDSLRIGHIDGDLFDPQLTWIR
                     NRGIEPDGAILVRPDRFVAWRSMSSASNPGQTLAKALTSVLGREVHMPEGAAA"
BASE COUNT          315 a          661 c          630 g          394 t
ORIGIN      
        1 tcacgagcac ccggctggac gcgatcatgt gcgattccca gagcatgaag gcgcgcatcg
       61 agcggggcct cagcggcaga ccggcctgat ccggatcggc tttcaccttg tccgcgtcaa
      121 ccgaatcgag cgacttctgc accgccttgc gggccggttc gcccgcggcc aggaaggcga
      181 ccatgtccgt cgtctgcccg tcgtccagat cgagttcggt cgcttctctc ttcagcgcgg
      241 gaagcgagac ccactcgccc ggtccggctt cgaccatcag cccttccggg ccagagcgca
      301 gcttcacttg acttcccctt cccggtggtt gatcgggaat ccggtcaaaa ggaacttcgc
      361 cggcggggtc gatccccact ggttgatgtc cttcatgccg ccggtccact tgcggggctt
      421 gtagtcacct tcgggcggca tcttctcgag ttcggcgcag cactcgatca tcgccccgtc
      481 ggcgtcgtgg aaatagagga agaggttgtt gcccggaccg tgccggctcg gtccccagat
      541 cgcccgctga cccctggccc ggagccggtc ggcggtgcgc ccgagggcgt tcaggtcttc
      601 caggctccag gcgtagtggg aaagctctgc ccggggtgcg atcacgaccg ccatgccgtg
      661 gtggtcgggg tcacagtgcc accagctggc ggtgcggccc agccggtcgg agaacctgaa
      721 accgaggcct tccgaaagga acctctcctc ccggcccatc cgtagcgcct tggttgaaac
      781 atgctcgaac tgcgaggggc gatcacccgg ctccggctcg gccaccttct ccatgccgag
      841 gaagatcttg aagacatggc cggccggcga gcggacgaag aaacagcggt caatgccggt
      901 ctcgccctcc tgaatttggg tgagaacatc tcccccggcc gcggcagcct gggcgcggac
      961 cgcctcaaga tcttcggcag tcgcgacctc gaggccgatg tggtcgtagc cccgcttgcc
     1021 ggactggatc aggatcagct cgtgatgacg gtcgttgcag gtcatgaacg aggtgccgcc
     1081 ctgccgctcg gtttcgacca gacccatcac ttcgctctgg aactcgacgg cacggtcaag
     1141 gtcacttacc cgcatcgcca tgtgaccgat tttggaaacg gcactcatgc tgctgctcct
     1201 tccggcatgt ggacttcccg gccaagtacc gaggtcaggg ccttggccag ggtctgcccc
     1261 gggttgctgg ccgagctcat gctacgccag gccacgaaac ggtcgggccg gacgaggatc
     1321 gctccgtcag gctcgatccc ccggttgcgg atccaggtca actgcgggtc gaagaggtca
     1381 ccgtcgatgt gtccgatccg caggctgtcc agcgggactt tggccgagcc ggccaggtca
     1441 ttcgcggctt gaagccattc gtcgccttct tcgccggcga tcaaaaggaa acggcctggt
     1501 ctgacgaggt ccttgatcgg ctgacggttg ccttccaggt catccagcca ggcatgcggc
     1561 aggggtgaac cgggacgggt tgaaggcaaa tatatccgca cctcatcggg attcggtgcc
     1621 ggcggacttc cgtccgcaac caccgctgcc gagtcgtagg cgtacccgta ttcgacgttc
     1681 agttcattga attccatcga ctgcgatcgc gctcccctcg ccaccttctc acggaactcc
     1741 tggtcggcgg gatcctcgtt ccagagtcgc ttcatcgccg cccagttttc ttcggtcgag
     1801 ttctcggggg tcactccgat ctcggccatc gtgccgagat ggttgatcgc attttcgagt
     1861 gagcgctggg cgttgcgggc gacgaccggt cgcctttccg gctcgtaggt ctcaagcagg
     1921 ctctcgccgg cttcgccctt gagcaccgac gcgatcttcc aggtcaggtt gtggacgtcg
     1981 tggatcgcgc tggtcaggcc
//
LOCUS       DAOQFI010000386         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34447_length_1998_cov_3.708698, whole genome shotgun sequence.
ACCESSION   DAOQFI010000386 DAOQFI010000000
VERSION     DAOQFI010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34447_length_1998_cov_3.708698"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLE93_07150"
     CDS             <1..702
                     /locus_tag="PLE93_07150"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH86861.1"
                     /translation="KPSARTDVPVGPDGRISYLQVHGLLAAGLTIDELRDRLTEDQDL
                     GLRLMIAGWKCHHDNHATVEQQGVPGLRRLFRQRTRWSQGNLQAMGLIDEMVTSRVHL
                     PARFEQVVFLLMPVWQMIVGASLIASIYLFFFEGVRFIHNLEEWWWFYLLYLLGFGGT
                     VMGSIAAKIGDRSIILGIIKGILTGQVYAFYTWLLWPVLLRSSVRQVIGRGSWAKTSR
                     EQITPADEPPASNPA"
     gene            832..>1998
                     /locus_tag="PLE93_07155"
     CDS             832..>1998
                     /locus_tag="PLE93_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNH86862.1"
                     /translation="MDLLLVPLDDAIVFPSVTATLQIDVGDEEQVFLLPRREGEFARV
                     GVIADVVEKGQSPRGDTVATVVGLRRGLAGVAHSDENDPDALRIEVQEIHDGHPEDEH
                     TRELVREYRAIVEEILELRNADSRIVSFLRNVTEPGELADTSGLSPDLSFDQKFELLD
                     TLNVTARLEKAVEFQRERLAELQVRSKIRDHVESGAQQQQREYFLREQMKSIRKELGE
                     DEGDVIEEYEQKIAEAGMPEAVEEQAVKELRRLERQGEQSGEGSMIRSYLDWLIAVPW
                     SKRSDEKLDPAYTREVLDADHAGLDDVKRRITEFVAVRKLRKEREAEADSRADGAILT
                     LVGPPGTGKTSIGESVAKALGREFVRISLGGVHDEAEIRGHRRTYIGALPGRIVR"
BASE COUNT          365 a          645 c          646 g          342 t
ORIGIN      
        1 aaaccgtcgg cgcggaccga cgtgcccgtg gggccggatg gccgcatcag ttacctccaa
       61 gtccatggct tgctggccgc cgggctgacg attgatgagt tgcgtgaccg gctgaccgaa
      121 gatcaggacc tcggacttcg cctgatgatc gccggctgga aatgtcacca cgacaaccat
      181 gcgacggtcg agcagcaggg cgtgcccggt ctgaggaggc tctttcgcca gcgcacccgc
      241 tggtcacagg ggaatctcca ggcgatgggc ctgattgacg aaatggtcac ctcccgggtt
      301 catctgccgg cccgtttcga acaggtggtc ttcctgctga tgccggtctg gcagatgatc
      361 gtcggcgctt ccctgatcgc ctcgatctat ctcttcttct tcgaaggcgt gaggttcatc
      421 cacaacctcg aggagtggtg gtggttctac ctcctctacc tgctcggttt tggcggcacc
      481 gtgatggggt cgatcgccgc aaagatcggc gaccgctcga tcatcctcgg gatcatcaag
      541 ggaatcctca ccggccaggt ctatgccttc tacacctggc tgctctggcc ggtcctgctg
      601 cgctccagcg tccggcaggt gatcggccgc ggatcctggg ccaagacctc ccgggagcag
      661 atcacccccg ctgacgaacc cccggcctcg aacccggcct gacccctcac ccgaggcttc
      721 ctgtatcggg gtgggaatcg ggttcgtccc gatgtccgag gcagttggtt gacagcgcaa
      781 caacttgcgc tacactgtgc gaaggactaa ctacagaaat tggacttact aatggatctt
      841 ctgctggttc cacttgatga cgcaatagtt ttcccctcgg tcacggcgac gctccagatc
      901 gacgtcggtg acgaggaaca ggtctttctg ctgccgcgcc gcgaaggcga gttcgcccgg
      961 gtaggggtga tcgccgacgt ggtcgagaag gggcagtccc cccgtggcga caccgttgcc
     1021 acggtggttg gcctgcgtcg cggcctggcc ggtgtggccc attccgacga gaatgatccg
     1081 gacgcgctcc ggatcgaggt tcaggagatc cacgacggcc accccgagga cgagcacacc
     1141 agggaactgg ttcgcgagta ccgggcgatc gtcgaggaga tcctcgaact cagaaacgcc
     1201 gacagccgca tcgtcagctt tctgcgcaac gtgaccgagc cgggcgagct cgccgacacc
     1261 tccggcctca gccccgacct cagcttcgac cagaagttcg agctgctcga cacgctcaac
     1321 gtgaccgccc gcctcgagaa ggcggtcgag ttccagcgcg agcgcctggc cgagctgcag
     1381 gtccgcagca agatccgcga ccacgtcgag tccggcgccc agcagcagca gcgcgagtac
     1441 ttcctgcgcg agcagatgaa gtcgatccgc aaggagctcg gcgaggacga aggcgacgta
     1501 atcgaggagt acgagcagaa gatcgccgaa gcggggatgc ctgaagccgt tgaggaacag
     1561 gccgtgaagg aactgcgccg gctcgagcgt cagggcgagc aatcgggcga gggctcgatg
     1621 atccgttcct acctcgactg gctgatcgcg gtcccgtgga gcaaacgctc cgacgagaaa
     1681 ctcgatccgg cctacacccg cgaggtactc gatgccgacc acgccggtct tgatgacgtg
     1741 aagcggcgga tcaccgaatt cgtcgcggtg cggaagctcc gcaaggagcg cgaggccgag
     1801 gccgacagcc gggctgacgg cgcgatcctg accctggtcg gaccgcccgg taccggcaag
     1861 acctcgatcg gcgagtcggt cgccaaggcg ctcggccgcg agttcgtgcg gatctcgctg
     1921 ggcggagttc acgacgaagc cgaaatccgc ggccaccgcc gcacctacat cggcgccctg
     1981 ccgggacgga tcgtccgt
//
LOCUS       DAOQFI010000387         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34458_length_1998_cov_2.740607, whole genome shotgun sequence.
ACCESSION   DAOQFI010000387 DAOQFI010000000
VERSION     DAOQFI010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34458_length_1998_cov_2.740607"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLE93_07160"
     CDS             complement(<1..1035)
                     /locus_tag="PLE93_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH86863.1"
                     /translation="MSGSPASSPRSSRGRRSRFPRRRHIGMRVWLAAGFTLVCFLTAG
                     ILYTFITDKSQDVLNERSTELAVGRTVRLADRLSEPDVDPKAELEAANGEGYTAWYFD
                     SDGDLVAPENPAATFPASKPYQEALDEALVGSRSSRDLKGGEIAIVGTTVGNEGEQVG
                     VVVGRYSRPVVIRSAIDTLRSESLRAALIAILIGTLVGVGIASVITGRLKRIAAKADD
                     LASGNFDVRLPVRGRDEIGDLARSLDSMRAALKESFGVLTADRDKLAAIFDGLGDAVM
                     VIDDDGSVRFHNSAANALLEQGGRAPEPMMSSVRRAAVEGFAAHPALRMGDRAYALQA
                     RSLPDEGAVLV"
     gene            1122..1838
                     /locus_tag="PLE93_07165"
     CDS             1122..1838
                     /locus_tag="PLE93_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH86864.1"
                     /translation="MRKLVFIPAWNEENSIEAVIADAREHVPDADVMVVDDGSTDRTA
                     ALARRAGALVASLPFNQGVGAAHQTAYIFALRHGYEICGQLDGDGQHPASEVTRLIAA
                     VEAGADLAVGSRFADDINLNIPEYKSTFARRIGQRLFSLLVSTSTRQTFTDTTSGMRA
                     LNERAMQLFADRYSSEFAEVESLQQAVRAGLVVREVPVHMLAREHGRSFIGPLTSAYY
                     IFKTLIVLLIGQLRPKQVEQ"
BASE COUNT          314 a          688 c          663 g          333 t
ORIGIN      
        1 gaccagcacc gccccttcgt cgggaagcga gcgggcctgg agcgcgtagg cgcggtcacc
       61 catgcggagc gccgggtgcg cggcgaaacc ctcgaccgcg gcccgtctga ccgaggacat
      121 catcggttcg ggggcccggc caccctgttc gagcagggcg ttcgcggccg agttgtggaa
      181 ccggaccgag ccgtcgtcgt cgatcaccat caccgcgtcg ccgagcccgt cgaaaatggc
      241 ggccagcttg tcgcgatcgg cagtcagcac gccgaagctt tccttcaggg cggcccgcat
      301 cgaatcgagc gaacgggcga ggtcgccgat ctcgtcacgg ccgcggaccg gcaggcggac
      361 gtcgaagttg ccggaagcga ggtcatcggc cttggccgcg atgcgcttga ggcggccggt
      421 gatcaccgag gcgatcccga ctccgaccag ggtgccgatc aggatcgcga tcaacgccgc
      481 gcgcaggctt tccgagcgca gggtgtcgat cgccgagcgg atcaccaccg gccgcgagta
      541 acggccgacc acgaccccga cctgctcgcc ttcgttgccg accgtcgttc cgacgatcgc
      601 gatttcgccg cccttcaggt cccgggagga gcgcgaaccg accagcgcct cgtcgagagc
      661 ctcctggtag ggcttgctcg cggggaaggt ggcagccggg ttctcggggg caaccaggtc
      721 gccgtcggaa tcgaaatacc aggcggtgta accctcgccg ttggcggctt cgagttcggc
      781 cttggggtcg acgtcgggct cgctgagccg gtcggccaga cggaccgtgc ggccgaccgc
      841 gagttcggtc gagcgttcgt tcagcacgtc ctggctcttg tcggtgatga aggtgtagag
      901 aatcccggcg gtcaggaaac agaccagcgt gaatccggcc gccagccaga cccgcatgcc
      961 gatgtggcgg cggcgcggga agcgtgatcg cctgcctcgg ctggatctag ggctgctggc
     1021 ggggctgccg ctcatgagcg gaccctagag gttgtagcca aactctgttc ccggtgtgga
     1081 gactctcggc cacaacctct atttcatgcc agaatcgccg cgtgaggaaa ctcgtcttca
     1141 ttccggcctg gaacgaggaa aactcgatcg aagccgtgat agccgacgcc cgggagcatg
     1201 ttccggacgc cgacgtgatg gtcgtcgacg acggttcgac cgaccgcacc gctgccctgg
     1261 cccgtcgggc cggtgccctg gtcgcctcgc ttcccttcaa tcagggggtt ggcgccgccc
     1321 atcagacggc ctacattttc gccctgcggc acggctacga aatctgcggc cagctcgacg
     1381 gcgacggcca gcatcccgcc tcggaggtca cccggctgat cgccgcagtc gaggccggag
     1441 ccgacctcgc ggtgggttcc cgcttcgccg atgacatcaa cctgaacatt cccgaataca
     1501 agtcgacctt cgcccggcgg atcggccagc ggctgttcag cctgctggtc tcgacctcga
     1561 cccggcagac cttcaccgac acgaccagcg gcatgcgggc cctgaacgaa cgggcgatgc
     1621 agctcttcgc cgaccggtac tcctccgagt tcgccgaggt cgagtcgctt cagcaggcgg
     1681 tgcgcgccgg cctggtggtg cgcgaagtcc cggtccacat gctggcccgc gagcacgggc
     1741 gctccttcat cggtccgctc acctccgcct attacatctt caagaccctg atcgtgctgc
     1801 tgatcggcca gctccgaccg aagcaggtgg agcaatgatc tgggccctcg cggccgatac
     1861 cgagccggtc gttcggctga ccccccaggc gcgcatcatc ggtgcggtcc tggcgatcgc
     1921 cttcatggcc ctgatccgcg acctgatccg ccgcgacaag ctgctcgagc gctactcgat
     1981 cgtctggttt ctgctcgg
//
LOCUS       DAOQFI010000388         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34483_length_1997_cov_2.827497, whole genome shotgun sequence.
ACCESSION   DAOQFI010000388 DAOQFI010000000
VERSION     DAOQFI010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34483_length_1997_cov_2.827497"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLE93_07170"
     CDS             <1..363
                     /locus_tag="PLE93_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNH86865.1"
                     /translation="LALWVPELGRIRSVLEGWFLLVWVKHKLPMPEVNVKVLGREVDC
                     LWRRLGVILELDGNEFHSDPLRKKLDVERQLFLEARGYLVIRVTYKEFAADPVGVVLR
                     IAEILRNREAELTGDRQS"
     gene            433..1353
                     /locus_tag="PLE93_07175"
     CDS             433..1353
                     /locus_tag="PLE93_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86866.1"
                     /translation="MVWILLAIVVSVAAGILGERRWPDKAGTGSRRGLLLILYFVLPP
                     IVFFNLVHVELSFGGGAGIAAGILTSICVGLLAWTLAVPVLKLSRPVAGAVIVSSILA
                     NTSYLGYPLVYTLMGGDALTDAVLFDVVVSYLTLMIGAFGVGAAFGTKAGEGFRARLK
                     AFFFKNPLLVAAILGLLAPESLAPDIAVEISRILVVLPLVVGFFAVGAILTEEVRDGV
                     MKVPPRPHRPLGAVIFCRLIAAPSLLLLFTLPVSGVPSSYYLLAVMPTGLNSMIVGHA
                     YGLDLRTCAEAIVYSTTIVLVGVAGYTLIA"
     gene            1391..1759
                     /locus_tag="PLE93_07180"
     CDS             1391..1759
                     /locus_tag="PLE93_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH86867.1"
                     /translation="MVHHVALEVRPDLMAAEGKFWVAAGFEPVAAPEGLGAGFDWYER
                     EGTQIHVMAVGQPGEPSRRGHVAIVAPDFEATLARLGEEGFEVSETRNLWGARRAKGR
                     TPAGHLVELMEFPPRPAEAG"
     gene            1756..>1997
                     /locus_tag="PLE93_07185"
     CDS             1756..>1997
                     /locus_tag="PLE93_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86868.1"
                     /translation="MIRRALLPAAILAAALIPGPASPAAAAGPEELVPDPDTPVVLIV
                     LDELPTAELMTADGRRINGIRFPNIARFARGATWYR"
BASE COUNT          285 a          637 c          674 g          401 t
ORIGIN      
        1 cttgccctct gggttccgga acttgggcgg atcaggtcgg tactcgaggg ctggttcctg
       61 ctggtctggg tgaaacacaa gctgccgatg ccggaagtga atgtgaaggt gctgggcagg
      121 gaggttgact gcctctggcg ccggctcgga gtgattctgg aactggacgg gaacgaattc
      181 cacagcgatc cgctccgcaa gaagctcgat gtggaaaggc agctctttct cgaagctcgc
      241 ggatacctgg tgatccgggt tacctacaag gaattcgcgg ccgatcctgt cggagtggtt
      301 ctccggatcg ctgagatcct tcgcaatcgt gaggcggaac tcacgggcga cagacagtct
      361 tgaagttatt cgttccatgt gcggataact tcaacgctgt catgggtgcg cggtcatccc
      421 tatggttagc cggtggtctg gattcttctg gcgatagtcg tttcggtggc ggccgggatt
      481 ctcggggagc ggcgttggcc tgacaaggcg gggacgggtt cacggcgggg gttgctgctg
      541 atcctgtatt tcgtcctgcc gccgatcgtc tttttcaacc tggtccacgt cgagttgagc
      601 ttcggtggcg gggcggggat cgcggccggc atcctcacct cgatctgcgt cgggttgctg
      661 gcctggaccc tggcggtgcc ggtcctgaaa ttgagccggc cggtagccgg ggcggtgatc
      721 gtttcctcga tcctcgccaa cacctcgtat ctcggatacc cgctggtcta cacgctgatg
      781 ggtggcgacg cgctgactga tgccgtcctc ttcgacgtcg tggtcagcta cctcaccctg
      841 atgatcggtg ccttcggggt cggtgcggcc ttcgggacca aggccggaga gggattccgc
      901 gcgcggctca aggcattctt cttcaagaac ccgctgctgg tcgccgcgat cctcggcctg
      961 ctggcgcccg aatccctcgc cccggacatc gcggtggaga tttcgcggat cctcgtggtg
     1021 ttgcccctcg tggtcggctt cttcgcggtc ggggcgatcc tcaccgaaga ggtccgggac
     1081 ggtgtgatga aggtgccacc ccgcccgcac cggccgctcg gcgcggtgat cttctgccgc
     1141 ctgatcgccg ccccgtcgct cctgctgctc ttcacccttc cggtcagcgg cgttccttcc
     1201 tcctactacc tgcttgccgt gatgccgacc ggcctcaatt cgatgatcgt cggccacgcc
     1261 tacggtctcg atctccgaac ctgcgccgag gccatcgtct actcgaccac catcgtcctg
     1321 gtcggcgtcg ccggctacac gctgattgcc tgatccgcct ttaccggcct cgatcgggga
     1381 agatcgggcc atggttcacc acgttgcgct ggaggtgcgg ccggatctga tggcggccga
     1441 gggaaagttc tgggtcgctg ccggattcga accggtggcc gcaccggaag gactcggggc
     1501 gggttttgac tggtatgaac gggaggggac acagattcac gtgatggctg tcggccaacc
     1561 gggcgagcct tcacggcggg gccacgtggc gattgtcgcc cccgatttcg aggcgacact
     1621 ggcccgtctc ggcgaggagg ggttcgaggt cagcgagacc cgcaatctct ggggagccag
     1681 gcgggccaag ggccggaccc ccgccggcca cctggtcgag ctgatggagt ttccgccccg
     1741 gccggcagag gccggatgat caggcgggcc cttctcccgg cggcgatcct cgccgccgcc
     1801 ctgatccccg gacccgcgag cccggccgcg gcagccgggc cggaggagct ggtgcccgac
     1861 cccgacacgc cggtcgtcct gatcgtgctc gacgagcttc cgaccgccga gctcatgacc
     1921 gccgacggca ggcgaatcaa cggcatccgc ttccccaaca tcgcccgatt cgcgcggggg
     1981 gcgacctggt accgcga
//
LOCUS       DAOQFI010000389         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34540_length_1995_cov_3.688660, whole genome shotgun sequence.
ACCESSION   DAOQFI010000389 DAOQFI010000000
VERSION     DAOQFI010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34540_length_1995_cov_3.688660"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLE93_07190"
     CDS             <1..437
                     /locus_tag="PLE93_07190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH86869.1"
                     /translation="SRVLELGIGTGETASRLLARFPDANVTGLDSDPDMLFKAREIVD
                     EVSLARMEDPLPDGPWDLILSVLSVHRLTDEQKQTLFRRVREQSKALVIGDFVSLGDD
                     RGETRVEGEAYPDTAEDLAAWCDGKIVWSEDDLVVIVAEYRR"
     gene            complement(526..996)
                     /locus_tag="PLE93_07195"
     CDS             complement(526..996)
                     /locus_tag="PLE93_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNH86870.1"
                     /translation="MDVLVLIDKLDDLVHNARPVPLTDQVRVDREEIYDLLDQMRATI
                     PEEIKQARWIVKERQEMLEEAKRESERVIREAREQQARLVSDEEVTKQAERAADEIVE
                     DARAREREIRLGAEDYADEILNTLEVNLQKFTAAVQRGRDRLAGRDEPLDEIEE"
     gene            complement(1100..1603)
                     /gene="coaD"
                     /locus_tag="PLE93_07200"
     CDS             complement(1100..1603)
                     /gene="coaD"
                     /locus_tag="PLE93_07200"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNH86871.1"
                     /translation="MNDRTERVAVCPGSYDPVTNGHLDIITRASRTFDKVVVGVVNQP
                     IRKKKTLFSAEERIAFIENATGHLGNVEVQAFYTLLVEFARENGASAIVKGLRAISDF
                     EYENEMAQLNYKLDPGVESIYVFARPEYSFLSSTGVKEMATFNGDISDLVPAEVATAL
                     ADRLGRS"
     gene            complement(1600..>1995)
                     /locus_tag="PLE93_07205"
     CDS             complement(1600..>1995)
                     /locus_tag="PLE93_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNH86872.1"
                     /translation="IEAISRGAGPAVLVDRETRPAMGNVHNLGLTGEAELVRAEAPEW
                     IANVPDGSFDLVFLDPPYRDATRIAGELDPHLARILAPGGRVIAESDRRGPLEFPSLE
                     TVRERRYGRTVVTFHRVPSQPPQDPTGTE"
BASE COUNT          322 a          607 c          659 g          407 t
ORIGIN      
        1 cgtcccgcgt tcttgagctg ggaatcggca ccggtgaaac cgcttcgcgt ctgctggccc
       61 gttttcccga tgcgaatgtc accggcctgg attcggatcc cgacatgctc ttcaaggccc
      121 gggaaatcgt cgacgaggtc agtctcgccc gcatggagga tccgctcccg gacggtccgt
      181 gggacctgat cctctcggtc ctttcggtgc atcggctgac cgacgagcag aagcagaccc
      241 tgtttcgccg ggtccgcgag cagtcaaagg ccctggtcat cggcgatttc gtctcgctgg
      301 gcgacgaccg gggagagacc cgggtcgaag gcgaggccta ccccgacaca gccgaagacc
      361 tggccgcctg gtgcgacgga aagatcgtct ggtccgaaga cgacctagta gtcatcgttg
      421 cggaataccg aagatagagc cccacccaac cattccttga ttcgaatggg tggggcgggt
      481 tgctttggga gggccggcgc agaccggccc gactgcagtt aagtcttatt cctcgatttc
      541 gtcgaggggt tcgtcgcggc cggcaagcct gtcgcgaccg cgctggacgg cggcggtgaa
      601 cttctggagg ttcacttcga gggtgttgag gatctcgtcg gcgtaatcct cggcaccgag
      661 acggatctcg cgctcgcggg cacgggcgtc ttcgacgatt tcgtcggcgg cccgctcggc
      721 ctgcttggtg acttcctcgt cggaaaccag ccgcgcctgc tgctcgcgtg cctcgcggat
      781 tacccgctcg gattcacgct tggcctcttc gagcatctcc tgacgctcct tgacgatcca
      841 gcgcgcctgt ttgatctctt ccgggatcgt ggcacgcatc tggtcgagca gatcgtagat
      901 ctcctcgcgg tccacgcgca cctggtcggt aagcgggacc ggacgggcgt tgtggacgag
      961 gtcgtcgagc ttgtctatta gaacgaggac atccatgtgg agggaaccct atgcgtctcc
     1021 gtcgatttgc gggggaaatg tggccatgtg gtgaagattc tgcatggaat cttccattcc
     1081 tctttgtgac atagaggcac taactgcgac caagtctatc cgccagtgcg gttgccacct
     1141 cggccgggac cagatcgctg atgtcgccgt tgaaggtcgc catttccttg accccggtcg
     1201 aggaaaggaa gctgtactcc ggccgggcaa agacgtagat gctctcgacc ccgggatcga
     1261 gcttgtaatt gagctgggcc atctcgtttt cgtactcgaa gtccgagatc gcgcgcagcc
     1321 ccttgacgat cgcactggcg ccgttctccc gggcgaattc gaccagcagg gtgtagaagg
     1381 cctggacttc cacgttgccg agatgaccgg tcgcgttctc gatgaaggcg atccgctctt
     1441 cggccgagaa cagggtcttc ttcttgcgga tcggctggtt gacgactccc accacgacct
     1501 tgtcgaaggt gcgggaggcg cgggtgatga tgtcgaggtg gccgttggta accgggtcgt
     1561 agctcccggg gcagacggcg actctctcgg ttctgtcgtt cattcggttc cggttgggtc
     1621 ttgagggggt tgggacggga cgcgatggaa tgtaaccacc gtgcggccgt agcgtcgttc
     1681 ccgaaccgtc tcgagggacg gaaattccag tggtccgcgc cggtcagact cggcgatcac
     1741 acggcccccc ggggcaagga tccgggccag gtgggggtcc agttcgccgg cgatccgggt
     1801 cgcgtcccgg tatggcgggt cgagaaagac caggtcgaaa ctgccgtcgg gcacattggc
     1861 gatccattcc ggcgcctcgg cccggaccag ctcggcctcg ccggtcaagc cgaggttgtg
     1921 gacgttgccc atcgccgggc gggtttcgcg gtcgaccagc acagccggcc cggcaccccg
     1981 cgagatcgcc tcgat
//
LOCUS       DAOQFI010000390         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34543_length_1995_cov_3.131443, whole genome shotgun sequence.
ACCESSION   DAOQFI010000390 DAOQFI010000000
VERSION     DAOQFI010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34543_length_1995_cov_3.131443"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLE93_07210"
     CDS             <1..297
                     /locus_tag="PLE93_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86873.1"
                     /translation="MRIRLPRGRSLEGPILAGRKMGLATVRLNGQPFATVPLFAGRTV
                     EKPGLIDKLLGNPLWIAGLLLLALFAIIASLLLVRRRRERKARKRLQRVLRTRR"
     gene            294..1250
                     /locus_tag="PLE93_07215"
     CDS             294..1250
                     /locus_tag="PLE93_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HNH86874.1"
                     /translation="MIISVTLNAAIDRTVAVPNLRLGHRHRSVETRTVPGGKGINVAR
                     ALGLLGRPVIATGLVGGGNGDRVLKQLGEEGLLTDFIRIAEETRFNLALVDPTTGEQT
                     EVNERGPRVSPEELDAFIERLDYLAGGAKLCVLAGSLPPGIDVEFYARLVEALKQAGL
                     PVLLDSEGEAMSAGMKVGPDMITPNKLEAEELVGHEFESRAEMFGVLSELVEMGPAEA
                     AITLPEGCVAIVGEGADQRMLEAVIEPLEPISTVGSGDAFVAGYIAARYDGGSPEECL
                     AYGVACGAESTQHLGAGRIDRGRAESLLGNVEVRQLQLPAEV"
     gene            1637..>1995
                     /locus_tag="PLE93_07220"
     CDS             1637..>1995
                     /locus_tag="PLE93_07220"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNH86875.1"
                     /translation="MEIEIGRGKKGRRAYGFDDVAIVPSRRTRDPDDIDISWTLGPYR
                     FDLPLVASAMDGVVSPETAVLVHKLGGLGVLNLEGIWTRFEDAEEKLELIANSSKDEA
                     TALMQEIYKTPVKKKLIT"
BASE COUNT          354 a          631 c          666 g          344 t
ORIGIN      
        1 atgcgcatcc gtctgccgcg cggccgttca ctcgaaggcc cgatcctcgc cggccggaag
       61 atgggcctgg cgacggtccg gctgaacggg caacccttcg ccacggttcc gctattcgcc
      121 ggacgaacgg ttgaaaagcc gggtctgatc gacaagctgc tcggcaaccc tttgtggatc
      181 gccgggctgc tcctgctggc cctcttcgcc ataattgcct cgttgttgct ggtgcgcagg
      241 cgccgggagc gaaaagcgag gaagaggctt caaagagtgc tcaggacgag acgatgatca
      301 tctccgtaac cctgaacgcg gcgatcgacc gcacggtcgc cgtacccaat ctgcggctcg
      361 gtcaccggca tcgttcggtt gaaacccgca ccgtgcccgg cggcaagggc atcaacgtgg
      421 cccgcgccct cgggctgctc ggccggccgg tcatcgcgac cggactggtc ggtggcggta
      481 acggggaccg ggtgctgaag cagctcggcg aggaaggcct gctcaccgat ttcatccgca
      541 tcgccgagga gacccgcttc aatctggccc tggtcgatcc gaccaccggt gaacagaccg
      601 aggtaaacga acggggcccc agggtcagcc cggaagaact cgacgcgttc atcgaacggc
      661 ttgattacct ggccggtggc gccaagcttt gcgtgctggc cggcagcctg ccccccggga
      721 ttgacgtcga gttctacgcg cgtctggtcg aggcgctgaa gcaggccggc ctgccggtgc
      781 tgctcgactc cgagggtgaa gcgatgtcgg cgggcatgaa ggtcggcccg gacatgatca
      841 ctccgaacaa gctggaggcc gaggagctgg tcggtcacga gttcgagtcc cgggcggaaa
      901 tgttcggggt gctttccgag ctggttgaaa tgggaccggc cgaagcggcg atcaccctgc
      961 cggaaggttg cgtcgcgatc gtcggggaag gcgccgacca gcggatgctc gaagccgtga
     1021 tcgaaccgct cgaacccatt tccacggtcg ggtccggtga cgcgttcgtg gccggttaca
     1081 tcgccgcccg ctatgacggc ggcagcccgg aggaatgcct ggcctacggc gtcgcctgcg
     1141 gagccgagtc aacccagcat ctcggagccg ggcggatcga ccgcggccgg gccgaaagcc
     1201 tgctcggcaa cgtcgaggtc cgtcagctcc agctgccggc cgaagtttga agagcgcccg
     1261 gtccgccccc cggcttccgg gtgcggaggg gcggtgggac cgggggacag cagccggcgc
     1321 ggtttcctgt accccggtcg atcgatcgtc cgggcggccg gttctagatt gaatcctgcg
     1381 atacggggct tctcctcccc ggtcatgtgg cagtaatcgt gggttcggga tcggaagccg
     1441 gaattcacac cctccaggcg aagcctccat ccttttgtgt gggggcttcg ctcgtgcagg
     1501 tgccgggccg ccaaagacct cccgctttgc ggaacaacgg aaaacgccga agggtggaga
     1561 ggcgtagtct ctgatcaaac gtccgcaggg gcgcgtagtc tgcccctctt caccctcaga
     1621 gccctggagg acctaattgg aaattgagat cggccgtggc aagaaaggcc gacgcgctta
     1681 cggattcgac gatgttgcca tcgttccttc ccgacggacc cgagaccctg atgacattga
     1741 catcagctgg acgcttggcc cgtaccggtt cgacctgccc ctggtcgctt ccgccatgga
     1801 tggtgtcgtc agcccggaaa ccgcagttct cgtccacaag ctgggtggtc tcggtgtgct
     1861 caacctcgag ggcatctgga cccgcttcga agacgccgag gaaaagctcg agctgatcgc
     1921 gaactcgtcc aaggacgagg cgaccgccct gatgcaggag atctacaaga ctccggtcaa
     1981 gaaaaaactg atcac
//
LOCUS       DAOQFI010000391         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34809_length_1987_cov_3.578675, whole genome shotgun sequence.
ACCESSION   DAOQFI010000391 DAOQFI010000000
VERSION     DAOQFI010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34809_length_1987_cov_3.578675"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1135
                     /gene="cax"
                     /locus_tag="PLE93_07225"
     CDS             5..1135
                     /gene="cax"
                     /locus_tag="PLE93_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HNH86876.1"
                     /translation="MSEPTTATGKRRMPEWFIWPYWLVPFIPVAIILDFFVGASATLI
                     FVTAALGVIPTAALMGVATEELAAKSGPGLGGLLNVTFGNAPELIIALFALGAGLQEV
                     VKASIVGAVLGNILLIMGIGMLAGGWKREKQTFNQTAVSSQALMLFLAVATLVLPAVF
                     VLVHGGGLPHVEEEITDFGSDVNTISIVASAILLFTYAAGLLFSLRTHKSFFNPSGEE
                     HGSPLGWSVRKAVGMLAFAGLAVGVMAHILVGSLEEVSVSLGLSEFFVGIIIVAIVGN
                     AAESWVSILYATKNKMDLSVQIAIGSATQLALVVMPTLVIVSFFLGPNPMALVFNGYE
                     MASLVIAVMAAYYVVQEGESNWFEGLQLLALYTVLGVVFYFA"
     gene            complement(1137..1472)
                     /locus_tag="PLE93_07230"
     CDS             complement(1137..1472)
                     /locus_tag="PLE93_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNH86877.1"
                     /translation="MSFGSVYPHYVAKAEKKGRSKDEVDEIIRWLTGYDQEQLESQIE
                     KGVDIETFYGEAPELNPSRELITGVVCGVRVEEIEEPLMQEIRYTDKLIDELARGKSM
                     GKILRAPAG"
     gene            1582..>1987
                     /locus_tag="PLE93_07235"
     CDS             1582..>1987
                     /locus_tag="PLE93_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86878.1"
                     /translation="MRGLLATLLVSVVALTALSGCGTANSSNEPELPALPPIQHDLGP
                     VAYRELLGDTFELLNEYWNEQANRLGETMPPPRALVSFWTRAQDKGCGGEKLGWQNAQ
                     YCWQNRTIAWDGNWVYGVPYRHLGDAAVAFLLG"
BASE COUNT          313 a          656 c          599 g          419 t
ORIGIN      
        1 tcacatgtcc gagccgacca cagccacagg caagcgcagg atgcccgaat ggttcatctg
       61 gccctactgg ctggttccgt tcattccggt cgcgatcatc ctcgacttct tcgtcggcgc
      121 gagcgcgacc ctgatcttcg tgactgcggc gctgggtgtg atcccgaccg ccgccctgat
      181 gggtgtagcc acggaagagc tcgccgcgaa gtcggggccc ggactcggcg gcctgctcaa
      241 cgtgaccttc ggcaacgccc ccgaactgat catcgccctc ttcgcgctcg gggcgggcct
      301 tcaggaggtc gtaaaggcct cgatcgtcgg tgcggtgctc ggcaacatcc tcctgatcat
      361 ggggatcggc atgctggccg gcggctggaa gcgggagaag cagaccttca accagaccgc
      421 cgtctcatcc caggccctga tgctctttct cgcggtggca accctggttc tgccggcggt
      481 cttcgtgctg gtccacgggg gcggcctccc ccacgtcgag gaggagatca ccgacttcgg
      541 ctcggacgtc aacacgatct cgatcgtcgc ttcggcgatt ctgctcttca cctacgccgc
      601 cgggctgctc ttctcgctca ggacccacaa aagcttcttc aacccgtccg gcgaagaaca
      661 cggttcgccg ctcggctgga gcgtccgcaa ggcggtcggc atgctcgcct tcgccggtct
      721 cgcggtcggc gtgatggccc acatcctggt cgggtcgctc gaggaggttt cggtttcgct
      781 cgggctgtcc gaattctttg tcggcatcat catcgtcgcg atcgtcggca acgccgcgga
      841 gagctgggtg tcgatcctct acgcgacgaa gaacaagatg gacctttcgg tccagatcgc
      901 gatcggttcg gcgacccagc tcgccctggt cgtgatgccg accctggtga tcgtctcctt
      961 ctttctcggc ccgaacccga tggcccttgt cttcaacggc tacgagatgg ccagcctggt
     1021 catcgcggtc atggccgcct actacgtggt tcaggaaggc gaatcgaact ggttcgaagg
     1081 cctccagctg ctcgccctct acaccgttct cggcgtcgtc ttctacttcg cctgagctac
     1141 ccagcgggag cgcggagaat cttccccatc gacttgcccc gggcgagttc gtcaatcagc
     1201 ttgtcggtgt agcggatctc ctgcatcaga ggttcttcga tttcctcgac ccgtaccccg
     1261 cagaccaccc cggtgatcag ctcccgtgaa ggattgagct cgggtgcctc tccgtagaac
     1321 gtttcgatgt cgaccccctt ctcgatctgc gactcgagtt gctcctggtc gtaacccgtc
     1381 agccagcgga tgatctcgtc gacttcgtcc ttgctcctcc ccttcttctc cgccttcgcc
     1441 acataatgcg gatagacgct gccgaaactc atcctgtaaa tgcggtgacc ggtcaagact
     1501 ctcctcgctg gtttcgagga tcctagtcac ggagatacaa aatgatccgc gctcggggag
     1561 ccatgtgcga cgatcggaga gatgaggggg ctcctcgcca cactgctggt ttcggtggtt
     1621 gccttgaccg ctctgtcggg ctgcggtacc gcgaactcct cgaatgagcc ggaactgccc
     1681 gccttgccgc cgatccagca cgacctcggt ccggttgcct accgtgagtt gctcggtgac
     1741 actttcgaat tgctcaacga gtactggaac gagcaggcaa acaggcttgg cgagacgatg
     1801 ccgcctccgc gggcgctggt ttcgttctgg acccgggccc aggacaaggg ctgcggcggc
     1861 gagaagctcg gctggcagaa cgcccagtac tgctggcaga accggacgat cgcctgggac
     1921 ggaaactggg tctacggggt tccgtaccgg cacctcggcg atgcggcggt ggcgttcctg
     1981 ctcggtc
//
LOCUS       DAOQFI010000392         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34876_length_1985_cov_3.112435, whole genome shotgun sequence.
ACCESSION   DAOQFI010000392 DAOQFI010000000
VERSION     DAOQFI010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34876_length_1985_cov_3.112435"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1985)
                     /locus_tag="PLE93_07240"
     CDS             complement(<1..>1985)
                     /locus_tag="PLE93_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH86879.1"
                     /translation="LSRFNQNTLKWGIQAAYERLRAAGEFDPRLPDPPEPRPLETIQL
                     EVLKVALRARGDSSLTASYKTKIGNLIDYLEATDPPDFCFDGLDGVDFYKTRSKKIPE
                     LCDAFREAKTELLTRELGPQYWDLLSNLLSNYGTAYDRAKDRRGGLDFEDLQLKTLDL
                     LLRREVIADSYREQFAEIMVDEFQDTNRLQMDLIDKLRGEETTLFTVGDEMQAIYGFR
                     YADVELYRQRRAGISFRLPADAERRAAGERDRVTFEVTTLPLSANFRSQAPVIGAVNE
                     LGNQFESLIEGIRTDDEPGGETGGTRHEFEHLRVGWSPPEPGSGPAPPPEPKVEILLT
                     KAKAWQQVDLGPLSPAPTGDQYMTESRGQHEAEALALARHIRKAVDDGIPPGDIVVLL
                     RTKTRMWLFEAALKQAGLRPYVVGGTGFWETREGIDMRALLATVANPLDDEALAGALA
                     GPACGLSPSALWLLGSRRDHDQPLWPLVSNLADKPDPEISEEDNRRAGEFVATIRRLR
                     AKLAGLPLSAVVQTAVSETGYDLVNLMRDPSGSGLANIRRVAEIASEFEATEGRDLRG
                     LIEWIDDSQHSLVWRFPRYHNQIKNAAQLIVRPGQSAVFVHQGRVADVFGPGQYRLET
                     KNLPILSTLMGWYHGFDSPFKAEIYFVSTRQLTDLKW"
BASE COUNT          323 a          641 c          654 g          367 t
ORIGIN      
        1 cccacttcag gtccgtcagc tggcgggtgc tgacgaagta gatctccgcc ttgaacgggc
       61 tgtcgaagcc gtgataccag cccatcagcg tcgagaggat cgggaggttc ttggtctcga
      121 ggcgatactg accggggccg aacacgtcgg cgacgcgtcc ttgatggacg aagaccgccg
      181 actggcccgg ccggacgatc agctgggccg cgttcttgat ctggttgtgg tagcgcggga
      241 agcgccagac gaggctgtgc tggctgtcgt cgatccactc gatcagcccc cgcagatccc
      301 tgccctctgt cgcttcgaac tcgctggcga tctcggccac ccgccggatg ttggccagtc
      361 cggaaccaga cggatcccgc atcagattga cgaggtcgta gccggtttcc gaaaccgcgg
      421 tctggaccac ggctgaaagc ggcaagcccg ccagcttcgc gcgaaggcgc cggatggtcg
      481 ccacgaactc gccggcccgc cggttgtctt cctcggagat ctccggatcc ggcttgtccg
      541 ccaggttcga gaccagcggc cagagcggct gatcgtgatc gcgacggctg ccgagcaacc
      601 agagtgcact cgggctgagc ccgcaggccg gaccggccaa agcgccggcc agcgcctcgt
      661 catcgagcgg gttcgcgacg gtcgccagca acgccctcat gtcaatgcct tcgcgggtct
      721 cccagaaacc cgtgcccccg accacgtagg gcctcagtcc ggcctgcttg agcgcggctt
      781 cgaagagcca cattctcgtc ttggtcctga gcagaacgac gatgtcgccg ggcggtatcc
      841 cgtcgtctac ggccttgcga atgtgacggg ccagggccag cgcttcggct tcgtgctgcc
      901 cgcggctctc ggtcatgtac tggtctccgg tcggcgccgg tgagagcggt ccgagatcaa
      961 cctgctgcca ggccttggct ttggtcagaa ggatctcgac cttcggttcg gggggcgggg
     1021 ccggcccgga gcccggctcc ggcggtgacc agccgaccct gagatgctcg aactcgtggc
     1081 gggtgccacc ggtctctccg cccggttcat cgtcggtcct gatgccctcg attagtgatt
     1141 cgaactggtt gccgagctcg ttgaccgcac cgatcaccgg ggcctgggag cggaagtttg
     1201 cgctcagcgg gagagtcgtg acctcgaaag tgacccggtc ccgctcaccg gccgcgcggc
     1261 gttcggcatc ggcgggaagc cggaacgaga tgccggcacg ccgctgccgg tagagctcga
     1321 cgtcggcata gcggaatccg taaatcgcct gcatctcgtc gccgaccgtg aacagggtcg
     1381 tctcttcgcc acggagcttg tcgatcagat ccatctgcag gcggttcgtg tcctggaact
     1441 cgtcgaccat gatctcggcg aactgctcgc ggtaggaatc cgcgatcacc tcgcgcctaa
     1501 ggagcagatc gagagtcttg agctgtaggt cctcgaaatc gagccccccg cgccggtcct
     1561 tggcccggtc gtaggcggtg ccgtagttgg aaagcaggtt cgagaggaga tcccagtact
     1621 gcgggccaag ctcgcgggtg agcagttccg tcttggcctc ccggaaggca tcacagagct
     1681 cgggaatctt cttggacctc gtcttgtaga aatccacccc gtcgagcccg tcgaaacaga
     1741 agtcaggcgg gtcggtcgct tcgaggtaat cgatcaggtt cccgattttc gtcttgtatg
     1801 acgcggtgag gctcgaatcg cccctcgcgc ggagagccac tttcaggacc tccagttgaa
     1861 tcgtctccag cggacggggt tccggcgggt ccggaagcct gggatcgaac tccccggccg
     1921 cccgcagccg ttcgtaggcg gcctgaatcc cccacttgag ggtgttctga ttgaagcggg
     1981 agagc
//
LOCUS       DAOQFI010000393         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34884_length_1985_cov_2.829534, whole genome shotgun sequence.
ACCESSION   DAOQFI010000393 DAOQFI010000000
VERSION     DAOQFI010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34884_length_1985_cov_2.829534"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLE93_07245"
     CDS             <1..825
                     /locus_tag="PLE93_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH86880.1"
                     /translation="FNLGGEGQIYAGAPASALVILGLSGVPGPILIVLGLIAGMIGGG
                     IWGGIAGALRSRLGLSEIITTIMLNFIAFWIVSYLVRGPIQDPDGAGYPYTKEIPAKA
                     DLPVIGGSIPLGAILLVVAAIVVWVLLERTRAGISIKSLGSGEATGRFAGLRVERTGF
                     AVLLIAGVFGGLAGAASLMGNEGRLSDFFSPGWGFIGVMTALIGRGTAIGTLVAGLLI
                     GFLSNGITGAQSAAGIPTSTAQILIGVFVLFLIIVNTDVVATALRRAASSLKGVRG"
     gene            822..1754
                     /locus_tag="PLE93_07250"
     CDS             822..1754
                     /locus_tag="PLE93_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH86881.1"
                     /translation="MSDFLDILGSTALYATAVRLALPILFCALGEVYAERAGLVNVGL
                     EGMMLCGAFAGVIGSHYSGSPWIGALVGMIAAIGVAAIQAGVSINLSGDQIVVGIALN
                     LAVLGITSFLARAIWEGGDVPKVDGFSDFAMPLLSDIPVLGPILFKQGALIYVALVVA
                     VASWWILTRTSWGLRLRSCGEDPRAADSLGISVFAYRWKSMIICGALAGLGGVFLSLG
                     QLFTFSDNMSGGRGYIALAVVIIARWSPLRALWAALIFGICEALALRVQVTDINVPAE
                     LMLALPYVVTLIVYAGVIGKSRPPKALGREYSRG"
     gene            1747..>1985
                     /locus_tag="PLE93_07255"
     CDS             1747..>1985
                     /locus_tag="PLE93_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86882.1"
                     /translation="MAEKVETLRLAVVQPQVDGNAPASERVPEAVRYIAEAGEAGAQI
                     VLFPEGYPGPLRIDEAYDAARPIAEACREARVAAC"
BASE COUNT          261 a          682 c          683 g          359 t
ORIGIN      
        1 ttcaacctcg gcggtgaagg ccagatctac gccggtgccc ccgcctcggc gctggtgatc
       61 ctcggcctga gcggcgtccc gggaccgatc ctgatcgtgc tcggcctgat cgccggcatg
      121 atcggcggcg ggatctgggg cgggatcgcc ggcgccctcc ggtcgcggct cgggctctcc
      181 gagatcatca cgacgatcat gctcaacttc atcgcgttct ggatcgtcag ttacctggtc
      241 cggggcccga tccaggatcc ggacggggcc ggctacccgt acaccaagga gattcccgcc
      301 aaggccgatc tgccggtgat cggtggctcg atcccgctcg gcgcgatcct gctggtcgtc
      361 gcggcgatcg tggtctgggt gctgctcgag cggacccggg ccgggatctc gatcaagtcg
      421 ctcggctccg gcgaggcgac cggccggttc gccggcctgc gggtcgagcg gaccgggttc
      481 gcggtgctgc tgatcgccgg cgtcttcggc ggcctggccg gcgcggccag cctgatgggc
      541 aacgagggca ggctcagcga tttcttcagc ccgggctggg gtttcatcgg cgtcatgacc
      601 gccttgatcg gtcgcggcac cgcgatcgga accctggtcg cgggcctcct gatcggcttt
      661 ctcagcaacg ggatcaccgg cgcgcagagt gcggccggga tccccacctc gaccgcgcag
      721 atcctgatcg gcgtgttcgt cctgttcttg atcatcgtca acaccgacgt cgtggccacc
      781 gccttgcgaa gagccgcctc gagcctgaaa ggggtccgcg gatgagcgat ttcctcgaca
      841 ttctcggttc gaccgcgctt tatgcgaccg ccgtccgcct ggcgctgccg atcctcttct
      901 gcgcgctcgg cgaggtgtac gcggaacggg ccggcctggt caacgtcggg ctcgagggga
      961 tgatgctctg cggcgccttc gccggcgtga tcggctccca ctactccggc agtccctgga
     1021 tcggcgcgct ggtcggcatg atcgccgcga tcggggtcgc cgcgatccag gccggcgtgt
     1081 cgatcaatct ctccggcgac cagatcgtgg tcggcatcgc actcaacctg gccgtcctgg
     1141 ggatcacctc gttcctcgcc cgggcgatct gggagggtgg cgacgtgccc aaggtggacg
     1201 gcttctccga cttcgcgatg ccgctcctct ccgacatccc ggtgctcggc ccgatcctgt
     1261 tcaagcaggg cgccctcata tatgtggccc tggtcgtggc cgtggcttcc tggtggatac
     1321 tcacccgcac cagctggggc ctgcggctcc ggtcctgcgg cgaggacccc cgggcggccg
     1381 acagcctcgg catatcggtt ttcgcctacc gctggaagtc gatgatcatc tgcggggccc
     1441 tggccgggct cggtggggtg ttcctctcgc tggggcagct cttcaccttc tccgacaaca
     1501 tgagcggcgg ccgcggatac atcgccctgg cggtcgtgat catcgcccgc tggtccccac
     1561 tcagggccct ttgggcggca ctcatcttcg gcatctgcga agcgctggca ctgcgcgtgc
     1621 aggtgaccga catcaacgtg ccggccgaac tgatgctggc gcttccgtac gtggtcacgc
     1681 tgatcgtcta cgccggcgtg atcggaaaga gcaggccgcc aaaggcgctc ggaagggagt
     1741 actcacgtgg ctgagaaagt cgaaaccctg cgcctcgcgg tcgtgcaacc gcaggtcgac
     1801 ggaaacgctc cggcatcgga gcgcgtgccg gaggcggtgc gctacatcgc cgaggccggc
     1861 gaggccgggg cgcagatcgt cctcttcccg gagggctacc cgggcccgct ccgcatcgac
     1921 gaggcctacg acgcggcgag accgatcgcc gaggcctgcc gggaagcccg ggtcgcggcc
     1981 tgctg
//
LOCUS       DAOQFI010000394         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_34964_length_1982_cov_3.129735, whole genome shotgun sequence.
ACCESSION   DAOQFI010000394 DAOQFI010000000
VERSION     DAOQFI010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34964_length_1982_cov_3.129735"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLE93_07260"
     CDS             <1..754
                     /locus_tag="PLE93_07260"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNH86883.1"
                     /translation="HLLIFPALVGWDDPGAAFTAVIQLGTEAAVLIYFRKDLWNIARD
                     WTESIFKPEMRGELNPRMGWYLIIATIPISILGLAFQDQIETAARNLWLVGSMLIIFG
                     LVLGRADRVGTHQREVGELSFKDGVIIGLAQSLALIPGVSRSGATISAGLFLGLGREA
                     AARFAFLLAIPAVVLSGFYELYKLLSGKESFDEPMINIAVATLVAFVVGYAVIAWFLR
                     YLTSHSMSVFVWYRVIVGTGVLILTAAGLISV"
     gene            complement(857..1300)
                     /locus_tag="PLE93_07265"
     CDS             complement(857..1300)
                     /locus_tag="PLE93_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86884.1"
                     /translation="MIPFRDNLDLRGPIWGTLALLFVYLVLALIGDIPHMNAWQVLVG
                     LVGLWLFAPYVERRAGTPLFLTGFVIVAGATGFLVGAVDEASGPYAISFFLPVLATAG
                     VHIALAPRSKILCLIPVPFAMTFVEIPTIAMTIIWVALEMLLTAA"
     gene            complement(1332..>1982)
                     /locus_tag="PLE93_07270"
     CDS             complement(1332..>1982)
                     /locus_tag="PLE93_07270"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNH86885.1"
                     /translation="KPTHNDPLEPARVAMQVSRLGLSHAVITSVDRDDLPDYGASAFV
                     GVIRSIRAQAPDCKVEILTPDFRGQEMPLARVVHERPDVFNHNVETVPRLYPKARRGS
                     KFLRSARVLRLAREMGEGEVITKSGLMVGLGEEFDEMVETFGILREHGVQILTVGQYL
                     RPTVNHLPVVRYWHPEEFEALEKAAYEIGFESVAAGPLVRSSYHADESARKAAEAA"
BASE COUNT          347 a          635 c          659 g          341 t
ORIGIN      
        1 gcacctgctg atcttcccgg cgctggtcgg ctgggatgac ccgggcgccg ccttcaccgc
       61 ggtgatccag ctgggaaccg aagccgcggt gctgatctat ttccgcaagg acctctggaa
      121 cattgccagg gactggaccg agtcgatctt caaacccgag atgagggggg agctcaaccc
      181 ccgcatgggc tggtacctga tcatcgccac gatcccgatc tcgatcctcg gcctcgcctt
      241 ccaggaccag atcgagaccg ccgcccgaaa cctctggctg gtcggctcga tgctgatcat
      301 cttcggcctc gtgctcggcc gggccgaccg ggttggcacc catcagcggg aggtcggcga
      361 actctccttc aaggacggcg tgatcatcgg cctcgcccag tcgctggccc tgatccccgg
      421 cgtttcccgt tccggcgcca cgatcagcgc cggtctcttc ctcgggctcg gtcgcgaggc
      481 ggccgctcgt ttcgccttcc tcctggcgat cccggccgtc gtcctctccg gcttctacga
      541 gctatacaaa ctgctctcgg gaaaggaaag cttcgacgag ccgatgatca acatcgcggt
      601 cgccaccctg gtcgccttcg tggtcggcta cgcggtgatc gcctggttcc tcaggtacct
      661 gaccagccac tcgatgtcgg tcttcgtctg gtaccgggtc atcgtcggca ccggcgtcct
      721 catcctcacc gccgccggcc tgatctcggt ctgacccgaa attgctagtc gaggccgccg
      781 ctggcctcca gacgcctgca tcttgcctgg gttcaagtca ttagggctat gtccgagtga
      841 attgaacgga ggagggtcag gcggcggtga ggagcatttc gagggcgacc cagatgatcg
      901 tcatggcgat cgtggggatc tcgacgaagg tcatcgcgaa ggggaccggg atcaggcaga
      961 ggatcttcga gcggggagcg agggcgatgt ggacgccggc ggtggcgagg accggaagga
     1021 agaagctgat cgcgtagggg cccgaggcct cgtcgaccgc gccgaccagg aaaccggtgg
     1081 ctccggcgac gatcacgaaa ccggtcagga agaggggggt gccggccctt cgctcgacgt
     1141 atggggcgaa cagccagagg ccgaccaggc cgaccaggac ctgccaggcg ttcatgtggg
     1201 ggatgtcgcc gatcagggcc aggaccagat agacgaacaa aagggcgagc gtgccccaga
     1261 tcggaccacg caggtccagg ttgtcgcgaa acggaatcaa ggaatcagcg agggtaccgg
     1321 gagaaagcgg ttcaagccgc ttcggcggct tttcgtgcgc tctcgtctgc gtggtaactg
     1381 ctgcgaacca gcggaccggc cgcgaccgac tcgaagccga tctcgtaggc ggccttctcg
     1441 agggcctcga attcctcggg atgccagtag cggaccaccg gcaggtgatt tacggtcggc
     1501 cgaaggtact ggccgaccgt gaggatctgg accccgtgct cccgcaggat gccgaaggtc
     1561 tcgaccatct cgtcgaactc ctcgccgagg ccgaccatca ggccggactt ggtgatcact
     1621 tccccttcac ccatctcgcg ggccaggcga aggaccctgg ccgagcgcag gaacttcgag
     1681 ccgcgccggg ccttcgggta aagccgcggc accgtctcga cgttgtggtt gaagacatcg
     1741 ggacgctcat ggaccacccg ggccaggggc atctcctggc cacggaagtc cggggtcagg
     1801 atctcgacct tgcagtccgg ggcctgggca cggatcgaac ggatcacgcc gacgaaggcg
     1861 gaagcaccgt agtccggcag gtcatcccgg tcaaccgagg tgatcaccgc atgcgaaagg
     1921 ccgaggcgac tcacctgcat cgcgacccgg gccggttcca gcggatcgtt gtgggttggc
     1981 tt
//
LOCUS       DAOQFI010000395         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35126_length_1977_cov_3.090010, whole genome shotgun sequence.
ACCESSION   DAOQFI010000395 DAOQFI010000000
VERSION     DAOQFI010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35126_length_1977_cov_3.090010"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLE93_07275"
     CDS             <1..354
                     /locus_tag="PLE93_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86886.1"
                     /translation="TDVIHGWNVPELTGKAQAVPGKTNEVYFRADDPGTYTSRSSVFS
                     GQGYDTMEIEVQVVPTDEYEAATIALKKDIQDAQDKVEAEFKASKAKAVVATAEGKKM
                     KAEINKDDIESADSQ"
     gene            351..>1977
                     /locus_tag="PLE93_07280"
     CDS             351..>1977
                     /locus_tag="PLE93_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNH86887.1"
                     /translation="MSRTTSYPQTMPPTRPEISSDGPGSRPSRWAELASSADHKDIGR
                     LLIATSFGSIFLAALELLLMRVQLAVPENVFLKGVTFDRLLSVYGETAIFLVALPLII
                     GLLYYVVPLQIGSRTTAIPRVGQIGLWLFIFGGLLLYGSFLFTPPEAGINPLPPFSDA
                     TFTPNNGVDVWIGSTGLITLGLLMIAIDLLATLHNLRAPGMAWRRAPLFTWAGAIVTW
                     LFVVIAPIFLAAISMLLIDRLYDGGFFAGDAGGSPILWQHLSYIFFTGVYMAVSITFL
                     AAIGEIIQTFTGRELPRKAVVWSFVVIAIVGTLAWTQNMLTAPIGSGWKYYGMLMSLA
                     LIVPFGLIFYNLISTLGSSDFHMRAPLRFALGALSLASIGLAAEIAQSTIAVATQLSE
                     TYDAWAATHFTLIGFVLFGGFAAVTYWYPKMTGHDLNEDRAAKSFGLMFVGTLIALLP
                     LFAAGVSGQVTDSYRYFSYEDLGVYNAISAVGTLLLFVGVVLAVGNLVKSRSEEPAAT
                     PDPYRGDSLEWLALSPPPAHNFDVLPDVRSTRPM"
BASE COUNT          314 a          730 c          555 g          378 t
ORIGIN      
        1 accgacgtga tccacggctg gaacgttccc gagctgaccg ggaaggccca ggccgttccg
       61 ggcaagacca acgaggtcta cttccgcgcc gatgacccgg gcacctacac gagccgctcc
      121 tcggtcttct cggggcaggg ctacgacacg atggaaatcg aagtccaggt cgtgcccacc
      181 gatgaatacg aggccgcgac catcgccctg aagaaggaca tccaggacgc ccaggacaag
      241 gtcgaagccg aattcaaggc cagcaaggcg aaggcggtcg tggccaccgc cgaaggcaag
      301 aagatgaagg ccgaaatcaa caaagacgac atcgaaagcg ctgactccca atgagccgca
      361 caaccagcta cccccagacg atgccgccga cccggcccga gatctcctct gacggtcccg
      421 gctcacgccc ttcgcgctgg gccgaactcg cttccagcgc cgatcacaag gacattggcc
      481 ggctgctgat cgcaacctcc ttcggttcga tcttcctcgc cgcgctcgaa ctgcttttga
      541 tgcgggttca gctggcggtc ccggagaacg tcttcctcaa gggagtcacc ttcgaccgtc
      601 tgctctccgt ttacggcgag accgcgatct tcctggtcgc cctgccgctg atcatcggcc
      661 tgctctacta cgtggttccg cttcagatcg gcagccgcac caccgcgatc ccgcgggtcg
      721 gccagatcgg tctttggctg ttcatcttcg gtggcctcct gctttacggc tcgttcctct
      781 tcaccccgcc ggaagccggc atcaacccgc ttcccccctt ctccgacgcc actttcaccc
      841 ccaacaacgg cgttgacgtc tggatcggct cgaccgggct gatcaccctc ggcctgctga
      901 tgatcgcgat cgacctgctc gccaccctgc acaacctgcg cgcccccggc atggcctggc
      961 gccgggcgcc gctcttcacc tgggccggcg cgatcgtgac ctggcttttc gtggtgatcg
     1021 ccccgatctt cctcgcggcc atcagcatgc tgctgatcga ccgcctctat gacggtggct
     1081 tcttcgccgg tgacgccggc ggctcgccga tcctctggca gcacctcagc tacatcttct
     1141 tcaccggcgt ctacatggcc gtctcgatca ccttcctcgc ggcgatcggg gagatcatcc
     1201 agaccttcac cggccgggag ctgcctcgca aagccgtcgt ctggtccttc gtggtcatcg
     1261 cgatcgtcgg caccctggcc tggacccaga acatgctgac cgcaccgatt ggcagcggct
     1321 ggaagtacta cgggatgttg atgtcgctgg ccctgatcgt tcccttcggc ctgatcttct
     1381 acaacctgat ctcgaccctc ggctcgagcg acttccacat gcgggcaccg ctgcgcttcg
     1441 cgctcggcgc gctttcgctg gcttcgatcg ggcttgccgc cgagatcgcc cagtcgacca
     1501 tcgccgtcgc gacccagctt tcggagacct acgacgcctg ggccgccacc cacttcacgc
     1561 tgatcgggtt cgtgctcttc ggcggcttcg ccgcggtcac ctactggtac ccgaagatga
     1621 ccggccacga tctgaacgag gaccgtgccg ccaagtcctt cggcctcatg ttcgtcggca
     1681 ccctgatcgc cctgctgccg ctgttcgcgg ccggtgtctc gggccaggtc accgactcgt
     1741 accgctactt cagctacgaa gatctcgggg tctacaacgc gatctccgcc gtcggcacgc
     1801 tgctcctctt cgttggtgtc gtcctcgcgg tcggcaacct ggtcaagagc cgcagcgaag
     1861 agcctgccgc cacgcctgac ccgtaccggg gcgattcact cgagtggctg gcgctctcgc
     1921 cgcccccggc ccacaacttc gacgtcctgc cggacgttcg cagcacccgc ccgatgc
//
LOCUS       DAOQFI010000396         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35135_length_1977_cov_2.630073, whole genome shotgun sequence.
ACCESSION   DAOQFI010000396 DAOQFI010000000
VERSION     DAOQFI010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35135_length_1977_cov_2.630073"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..338)
                     /locus_tag="PLE93_07285"
     CDS             complement(150..338)
                     /locus_tag="PLE93_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86888.1"
                     /translation="MKLSTAARSALTFIVVMIGLAIVGAIAGINVGPVEFVIWLVVLV
                     IGLALIVTGTNRSKQIEN"
     gene            complement(343..1143)
                     /locus_tag="PLE93_07290"
     CDS             complement(343..1143)
                     /locus_tag="PLE93_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNH86889.1"
                     /translation="MSTTIAILSQKGGTGKTTLTRSLSEGLRRAGLSVLAIDADPQGN
                     LSEYFGIPADAFPTLGEVLAGRERAVDAIHTGPGGGEVIPANLGLAEAELMLAGKIGR
                     ELTLRNALREPKRRFDVILIDCPPTLGLLTVNALVASDKAILSTQAEHFSEQGAEQAM
                     EVIGLARENLNPELELLGLLLNIADMRTNHARSTLETMREQFGDLVFDTVIRRSIVYP
                     ESARAAKPIFDYRKRKSLDYAALTVEVLERLDYPAALAEAKSALAELP"
     gene            1203..1712
                     /locus_tag="PLE93_07295"
     CDS             1203..1712
                     /locus_tag="PLE93_07295"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH86890.1"
                     /translation="MGPVFTEVDIDAPREEIFDYLLDFDTRPFLYGDSVENFRLLRLD
                     SRGIGAGARWQFKRKKAWADSAITATEAPRRISERGSTGSYNRNVTGTEWEMEEISTG
                     VTRVRITFWIVPAGFSRVHNRLTGGAGWHRRRLDKVGKRLREAIESARREKVEVPVAG
                     GNRHATGVA"
     gene            1759..>1977
                     /locus_tag="PLE93_07300"
     CDS             1759..>1977
                     /locus_tag="PLE93_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86891.1"
                     /translation="MERLSKSNLFRGLVLALVVAISGLALAACGASHEEKEGLAEGEI
                     VELGPLQYNVLFTRPLNRNDIEDSQYLVG"
BASE COUNT          348 a          636 c          648 g          345 t
ORIGIN      
        1 ggcgccgacg tccccgagct ctgagatgga cctgctcgtc cggatcggcc tgatcaacta
       61 accccagtcg ggccggtctt cgccggccct cccatcgaaa cccgcccctc ccactcgcag
      121 agacgaatgg ttgggaggga ctccgtttgt cagttctcga tctgcttgga ccggtttgtg
      181 ccggtcacga tcagtgccaa gccgatcacg agcacgacca accagatgac gaactcaacg
      241 ggtcccacgt ttatgcctgc gatcgctccg acgatcgcca ggccgatcat tacgacgatg
      301 aaggtaagtg cgcttcgtgc ggcagtcgag agtttcatgc gatcagggta gctcggccag
      361 ggcggacttc gcctcggcca gcgctgccgg gtagtcgagc cgctcgagga cttcgaccgt
      421 gagggccgcg tagtcgagcg actttcgctt gcggtaatcg aagatcggct tggctgcccg
      481 ggctgattcg ggatagacga tcgaacgccg gattaccgtg tcaaagacga ggtccccgaa
      541 ttgctcgcgc atggtctcca gggtgctgcg ggcgtgattg gtccgcatgt cggcgatgtt
      601 gagcagcagc ccgagcaact cgagctccgg attcaggttc tcccgggcca ggccgatcac
      661 ttccatcgcc tgctcggccc cctgctcgga gaaatgctcg gcctgggtcg agaggatcgc
      721 cttgtccgac gcgaccaggg cgttcacggt gagcaggccc agggtcggcg ggcagtcgat
      781 caggatcacg tcaaaacgcc gcttcggctc ccgcagggcg ttacggaggg tgagctcgcg
      841 cccgatcttg ccggccagca tcagctcggc ctcggctagg ccgaggttgg cggggatcac
      901 ctcgccgccg ccggggccgg tgtgaatcgc atcgaccgcc cgttcccggc cggccagcac
      961 ttcaccgagg gtggggaagg catcggccgg gatcccgaag tactcggaaa ggtttccctg
     1021 ggggtcggca tcgatcgcga gaacggaaag tcccgcccgg cgcaggccct cggagagcga
     1081 ccgcgtcaac gtggtcttcc cggttcctcc cttctgggaa aggatcgcga tcgtcgtgga
     1141 caccggcaca ccctatcgcc ggtcgggcac gcatcggcac gcaggtcgta gaatccggat
     1201 cgatgggtcc cgtattcact gaagtcgaca tcgacgcgcc gcgcgaagag atcttcgact
     1261 acctcctgga cttcgacacc cgcccgtttc tttacggcga ctcggtcgag aactttcggc
     1321 tcctgaggct ggattcgcgg ggcatcggcg ctggcgcccg ctggcagttc aagcgcaaga
     1381 aggcctgggc cgattcggcg atcaccgcga ccgaagcccc ccggcggatt tccgaacgcg
     1441 ggtccaccgg cagctacaac cgcaacgtga ccggcaccga gtgggagatg gaagagatct
     1501 caaccggggt aacccgggtg cggatcacct tctggatcgt gccggccgga ttctcccggg
     1561 ttcacaaccg cctgaccggc ggcgcgggct ggcacaggcg ccgtctggac aaggtcggca
     1621 agcgtcttcg ggaggcgatt gagtcggctc gcagggagaa ggtcgaggtt ccggtcgccg
     1681 gcggcaaccg acacgcgacc ggtgtcgcct aggaaccgct cagcacgtcc ggataaggcc
     1741 gaataaactc ccgcaccgat ggaaagactt tcgaagagca acctgtttcg tggcctcgtc
     1801 ctggccctcg tagtcgctat ctccgggctc gcgctcgccg cttgcggtgc cagccatgag
     1861 gagaaggagg gtctcgccga aggtgaaatc gtcgagctcg gcccgctgca gtacaacgtg
     1921 ctcttcaccc ggccgctgaa caggaacgac atcgaggatt cgcagtacct ggtcggc
//
LOCUS       DAOQFI010000397         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35329_length_1971_cov_3.675365, whole genome shotgun sequence.
ACCESSION   DAOQFI010000397 DAOQFI010000000
VERSION     DAOQFI010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35329_length_1971_cov_3.675365"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..>1971)
                     /gene="uvrA"
                     /locus_tag="PLE93_07305"
     CDS             complement(124..>1971)
                     /gene="uvrA"
                     /locus_tag="PLE93_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNH86892.1"
                     /translation="IDINTPWQELTQKERDVFLYGTGTERHTIRTKSRAGRQRSYQVK
                     FEGLVNNLERRFQESDSEAVREKIEDYMAEQPCPSCHGARLRPESLAVTVGGLSIRDF
                     TDMSATAAREWIGNLDLTATERAIARLIVREVEERLSFLVNVGIGYLSLARSARTLSG
                     GEAQRIRLATQIGSSLVGVMYVLDEPSIGLHQRDNEKLIATLERLRDLGNTVIVVEHD
                     EGTMRAADYLVDLGPGAGEHGGSVVAAGTPVEVSANPDSLTGAYLVGSEQIAVPESRR
                     SWEKALTVKGARQHNLTGVDVEFPLGVLTCVTGVSGSGKSTLVNETLFPAVANRLHKA
                     KLRPGPHDRIDGMEEIDKIINIDQSPIGRTPRSNPATYTGVFDQIRQLFAATQESRAR
                     GYKPGRFSFNVKGGRCEVCKGDGQIKIEMHFLPDVYVPCEQCHGHRYNRETLEVRFKG
                     KSIADVLEMSIEEAYPFFESIPKVARRLKTLNDVGLGYVRLGQPATTLSGGEAQRIKL
                     AAELSKVATGNTLYILDEPTTGLHFGDVRRLLEVLGRLVDAGNTVVVIEHDLDVIKSA
                     DWIVDLGPEGGDGGGHIVATGTPEEVARVQGSYTGDFLRGVLEPVALAA"
BASE COUNT          295 a          644 c          660 g          372 t
ORIGIN      
        1 cgatcaggac ttcgacgatc tccgccggtc cggccaccga gtcgccgtag ggaccgggtt
       61 cgccgggggc ttcggtttcg atcagtttcg gggcttcgcc gtaagcggtt acctgggcag
      121 ctttcatgcc gccaaagcta ccggctcgag gaccccgcgc aggaagtctc cggtgtagga
      181 gccctggacc ctggcgactt cctcgggcgt tccggtggcg acgatgtgac cgccaccgtc
      241 gccgccctcg gggccgagat cgacgatcca gtcggcgctc ttgatcacgt cgaggtcatg
      301 ctcgatgacc acgacggtgt tgccggcatc aaccagcctg ccgagcacct cgagcagccg
      361 tcgcacgtct ccgaagtgaa ggccggtggt cggctcgtcg aggatgtaaa gcgtgttgcc
      421 ggtcgcgacc ttggagagct cggcggccag cttgatccgc tgcgcctcgc caccggagag
      481 agtcgtggcc ggctggccga gccgcacgta gccgagcccc acgtcattca gggtcttcag
      541 gcgacgggcc accttcggga tcgattcgaa gaagggatac gcctcctcga ttgacatctc
      601 caggacatcg gcgatcgact tgcccttgaa ccggacctcc agggtttccc ggttgtaacg
      661 gtggccgtgg cattgctcgc agggcacgta cacatcgggc aggaagtgca tctcgatctt
      721 gatctgaccg tcgcccttgc agacctcgca gcggccgccc ttgacgttga agctgaaccg
      781 gcccggcttg tagccgcggg cccgggattc ctgggtggcc gcgaaaagct gccggatctg
      841 gtcgaagacc ccggtgtagg tggccgggtt cgagcgcggc gtgcggccga tcggcgactg
      901 gtcgatgttg atgatcttgt cgatctcctc catcccgtcg atgcggtcat gcggcccggg
      961 cctcagcttt gccttgtgga ggcggttggc cacggccggg aacagcgtct cgttgaccag
     1021 ggtcgatttg ccggaaccag aaacgccggt cacgcaggtc aggaccccga gcgggaactc
     1081 gacgtccacc ccggtcaggt tgtgctggcg ggctcccttc acggtcaggg ccttttccca
     1141 gctgcgccgg ctctccggca ccgcgatctg ctcggagccg accaggtagg cgccggtcag
     1201 tgaatcgggg ttggcggaaa cctcgaccgg cgtgccggcc gcgaccacgc tgccaccgtg
     1261 ttcgccggcc ccgggcccga ggtcgaccag gtagtcggcc gcccgcatcg tgccttcgtc
     1321 gtgctcgacc acgatcaccg tgttgccgag gtcacggagc ctttccaggg tcgcgatcag
     1381 cttttcgttg tcacgttgat gaaggccgat cgagggttcg tccagcacgt acatgacgcc
     1441 gaccaggctg gagccgatct gggtcgccag ccggatccgc tgggcctccc cgccggaaag
     1501 ggtccgggcg gaccgggcca gagagaggta gccgatcccc acgttgacca ggaaggaaag
     1561 ccgctcttcg acctcccgca cgatcagccg ggcgatcgcc cgttcggtgg cggtgagatc
     1621 caggttcccg atccattcgc gggccgcggt ggccgacatg tccgtgaagt cccggatcga
     1681 gagacccccg accgtgaccg cgaggctttc cggcctgagc cgggcaccgt gacagctcgg
     1741 gcagggctgc tctgccatgt agtcctcgat cttctcccga accgcttcgg agtccgattc
     1801 ctggaagcgg cgttcgaggt tgttgaccag gccttcgaac ttgacctggt agctgcgctg
     1861 gcgtccggcc cggctcttgg tccggatcgt gtggcgttct gtaccggtgc cgtagaggaa
     1921 cacgtcccgt tccttctggg tcagttcctg ccagggcgtg ttgatgtcaa t
//
LOCUS       DAOQFI010000398         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35416_length_1969_cov_2.208464, whole genome shotgun sequence.
ACCESSION   DAOQFI010000398 DAOQFI010000000
VERSION     DAOQFI010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35416_length_1969_cov_2.208464"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLE93_07310"
     CDS             <1..525
                     /locus_tag="PLE93_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH86893.1"
                     /translation="TCLVDRNGIDVRRWRNWRLMMEEEQQEQSGDGNGTGADEGGDRT
                     LQAWLAGIAIGCLVVAAMIVAYEIGKNNPDQEAAVKKPVAAAGSSGSPAPASPEASTF
                     ASTCGSCHTLAAADTDATVGPNLDDLQPDSAMVMAAIENGGAGSGVMPAGLLSGEEAQ
                     AVADYVAKSAGSGK"
     gene            587..1276
                     /locus_tag="PLE93_07315"
     CDS             587..1276
                     /locus_tag="PLE93_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86894.1"
                     /translation="MILGLAALLFLIPAAPARAHSNSPHLFESVPDQITPAHLAHGIE
                     VEMIDHDANLRLVNKSGNEVIVMGYDGEPYARLAANGEVFLNQRSPAYFLNQDRYATT
                     PVEASADPEAPAKWVKQSDDGTLIWFDRRSHSMSDQVPAVVDDTSVSQPVRDYQIPMR
                     VNGEPAKLGGTLYWNGQSEFPMGIVAGLLIVTAMCAGLGFLGIEALRRAEPESHGASG
                     GEVAPSPGPPQ"
     gene            complement(1235..1570)
                     /locus_tag="PLE93_07320"
     CDS             complement(1235..1570)
                     /locus_tag="PLE93_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86895.1"
                     /translation="MSRQGAQCDWCGVELTEEMGYRLLWPDKSLGTAFCRLEHIVPFL
                     MQKDQWHIWKAVTVPADAPSLSTATGDELGENALYLVHHRGEHRITDSFENKEALLGW
                     ARAGGHFAP"
     gene            complement(1567..>1969)
                     /locus_tag="PLE93_07325"
     CDS             complement(1567..>1969)
                     /locus_tag="PLE93_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH86896.1"
                     /translation="TILIAHVAFNIAIVIIVVQARVSGFDESLEEAAADLGAPPWITF
                     WKVTMPLILPGIFSAFLLSFALSIDDYIITSFVAGQTLTFPLWVAGAVKVGIPPQVFV
                     MGTLIFVSGVLIALASLILQRWGDHGEAVRR"
BASE COUNT          375 a          643 c          636 g          315 t
ORIGIN      
        1 acttgtctcg tcgatcggaa tggcattgat gttcgccgct ggcgtaactg gaggttgatg
       61 atggaagaag aacagcaaga acagtccggg gacggaaacg ggaccggcgc ggacgaaggg
      121 ggcgaccgga cgcttcaggc ctggctggcc ggcatcgcga tcggctgcct ggtagttgcg
      181 gcgatgatcg tcgcctacga gatcggcaag aacaaccccg atcaggaggc cgcggtcaag
      241 aagccggtcg ccgccgcagg gtcctccggc tcacctgccc cggccagtcc ggaagccagc
      301 accttcgctt ccacctgcgg cagctgccac accctcgcgg cggccgacac cgacgccacg
      361 gtcgggccca atctggatga cctccagccc gactcggcga tggtgatggc agccatagaa
      421 aacggcggcg ccggctccgg cgtgatgccg gcaggcctgc tctcgggtga ggaagcccag
      481 gcagtcgccg attacgtggc caagtcggca ggttccggga agtagcgcca aagtggaccg
      541 gcatcgacga gcatcgaaag ccgggtcccc gatcttcgtg cgagcgatga tcctgggcct
      601 tgccgccctg ctctttctga tcccggcggc gccggcccgc gcccacagca actcgcctca
      661 tctgttcgaa tcggtcccgg accagatcac ccccgcgcat ctggcccacg ggatcgaagt
      721 cgaaatgatc gaccacgacg cgaacctgcg cctggtcaac aagagcggca atgaagtgat
      781 cgtcatgggc tacgacggcg agccgtacgc ccggctcgcg gcgaacggag aggtcttcct
      841 caaccagcgg tcacccgcct atttcctcaa ccaggaccgt tacgcgacga ccccggtcga
      901 agcttccgca gatcccgaag ctccggccaa atgggtgaag cagagtgatg acgggaccct
      961 gatctggttc gaccggcgca gccactcgat gagcgaccag gtccccgcgg tggtcgatga
     1021 cacttccgtc tcgcagcccg tacgcgatta ccagatcccc atgcgtgtaa acggtgagcc
     1081 ggccaagctt ggaggaacgc tctactggaa cggccagagc gagttcccga tggggatcgt
     1141 ggccggcctg ctgatcgtca cggcgatgtg cgccggactc ggatttctgg gcatcgaagc
     1201 cctccgccgg gccgagccgg aaagccatgg cgcctcaggg ggcgaagtgg cccccagccc
     1261 gggcccaccc cagtagcgct tccttgttct cgaacgagtc ggtgatccgg tgttcacccc
     1321 ggtggtggac caggtagagg gcgttctctc ccagttcatc accggtcgcg gtgctcaggg
     1381 acggagcgtc ggcgggcacc gtcaccgcct tccagatgtg ccactggtcc ttctgcatca
     1441 ggaacggcac gatgtgttcg aggcggcaga aggcggtacc gaggctcttg tccggccaca
     1501 gcagccggta gcccatctcc tcggtcagct ccaccccgca ccagtcgcat tgcgcaccct
     1561 gtctgctcat ctcctcaccg cctctccatg gtcaccccag cgttgcagaa tcaggctggc
     1621 caatgcaatc aggaccccgc tgacgaagat cagggtgccc atcacgaata cctgtggcgg
     1681 gatgccgacc ttgaccgcac cggccaccca cagggggaaa gtcagggtct gaccggcgac
     1741 gaagctggtg atgatgtagt cgtcgatcga gagcgcgaag gagagcagga aggcagagaa
     1801 gatgccgggc aggatcagcg gcatcgtcac cttccagaag gtgatccagg gtggcgcccc
     1861 gaggtcggcc gcggcctcct ccagggattc gtcgaaaccg ctgaccctgg cctggacgac
     1921 gatgatcacg atcgcgatgt tgaaggcgac atgggcgatc aggatcgtg
//
LOCUS       DAOQFI010000399         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35621_length_1963_cov_1.724319, whole genome shotgun sequence.
ACCESSION   DAOQFI010000399 DAOQFI010000000
VERSION     DAOQFI010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35621_length_1963_cov_1.724319"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLE93_07330"
     CDS             complement(<1..437)
                     /locus_tag="PLE93_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH86897.1"
                     /translation="MSGIPDFSEVELDPVRTDPASEAELEALLEANRDPDGDEVHWET
                     PEGITLDPAYTSAALEGIDFLDTKPGFAPYLRGPYPSMYVNKPWTIRQYAGYSTAEES
                     NAFYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVAGDVGKAGVA"
     gene            complement(434..>1963)
                     /locus_tag="PLE93_07335"
     CDS             complement(434..>1963)
                     /locus_tag="PLE93_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH86898.1"
                     /translation="AIGSFRYDPLGEIARTGELPCSPEDALAAAGGLAAETARDWPKA
                     RALAVDTGIYVEAGATAGWELGIAVATGVEYLRAAAAAGLDPAEAASQIEFTLAVGPD
                     QFLEMAKFRAIRRLWARVLEESGVAAEARFSPTYARTSARMISAVDPWVNMLRVTTAA
                     FAAGTGGADGVTVTPFDRMIGQPGDLGRRIARNTQIILQDESSLGRIADPLAGSWYGE
                     ELTDEVAKAGWERFREIEREGGALAALRSGLIATTLGGLADEREDDLNHRRRVMTGIN
                     EFPLLGDDGTEPERADLSRVEGDSARLLEGPEVPGLGRLTEAAPSERLAVATSLASAG
                     ARIDELSAAVAGETFSLPALNVRHDATPFEVMRRAARRHEENGGDRPQIYLACMGSIA
                     SHVNYANWAKSFFEVAGVETVPSGALESNEDQAKTLRDGGFELVAICAGKNQSPEDVA
                     DLVGRLREAGASYIYMVNSTPELNEAATAAGADELVRNGVNLTEVLTATLGRLGVEVK
                     S"
BASE COUNT          278 a          665 c          695 g          325 t
ORIGIN      
        1 gccacaccgg ccttgccgac atcgccggca acgcgggggt ggtcggagtc gtagccgcgg
       61 tgagtggcca ggtcgaaggc gatcgaaagg cccttctggc cggccgcaag gtttcgacgg
      121 tagaaggcgt tcgattcttc ggccgtggag tagccggcgt actggcggat cgtccagggc
      181 ttgttcacgt acatcgacgg atacgggccc cggaggtagg gcgcaaagcc cggcttggtg
      241 tcgaggaaat cgatgccctc gagcgctgcc gaagtgtagg ccgggtcgag ggtgatcccc
      301 tcgggggtct cccagtggac ctcgtcgccg tccgggtcgc ggttcgcctc gagcagagct
      361 tcgagttccg cctcgctggc cgggtcggtc cggaccgggt cgagttcgac ctcggagaaa
      421 tcggggatgc cgctcatgac ttcacctcca cgccgagccg gccaagcgtg gcggtcagca
      481 cctcggtcag gttcactccg tttcggacca gttcatcggc gccggcggcg gtcgccgcct
      541 cgttcagttc cggggtcgag ttgaccatgt agatgtagct cgccccggct tcgcggagcc
      601 tgccgaccag gtccgccacg tcttccgggc tctggttctt tccggcgcag atcgcgacca
      661 gttcgaatcc cccgtcgcgc agcgtcttcg cctggtcctc gttgctttcc agggcgccgc
      721 tcgggacggt ttcgaccccg gcaacctcga agaagctctt ggcccagttc gcgtagttga
      781 cgtgactggc gatcgagccc atgcaggcga ggtagatctg gggccggtca ccgccgtttt
      841 cctcgtgccg ccgggcggcc cggcgcatga cctcgaaggg ggtcgcgtca tgacgcacgt
      901 tcagggccgg cagtgagaag gtttcccccg cgaccgcagc cgagagctcg tcgatgcggg
      961 caccggcaga agcgagtgag gtggccacgg ccagccgttc ggaaggcgcc gcttcggtca
     1021 gccttccgag tccgggcact tccgggcctt ccagcaatcg cgccgaatca ccttccaccc
     1081 tcgaaaggtc ggcccgctcc ggctcggtgc cgtcatcgcc gagcagcggg aactcgttga
     1141 tcccggtcat cacccggcgg cgatggttga ggtcatcctc gcgctcgtcg gcgagcccgc
     1201 ccagggtggt ggcgatcaat ccggaacgga gcgcggccag ggcgccgccc tcgcgttcga
     1261 tttcgcggaa ccgctcccag ccggctttgg ccacctcgtc ggtcagctct tccccgtacc
     1321 aggagccggc cagcgggtcg gcgatgcggc cgagtgagga ctcgtcctga aggatgatct
     1381 gggtgttgcg ggcgatccgg cggccgaggt cgcccggctg gccgatcatc cggtcgaagg
     1441 gagtgacggt caccccgtcg gcgccaccgg tgccggccgc aaaggcggcc gtggtaacgc
     1501 gaagcatgtt gacccagggg tcgacggcgc tgatcatccg ggccgaagtc ctggcgtagg
     1561 tcggggagaa ccgggcctcg gcagcgacgc cggattcctc gagcacgcgg gcccagagcc
     1621 ttcggatcgc gcggaacttg gccatctcga ggaactggtc gggtccgacc gcgagggtga
     1681 actcgatctg cgacgcagct tcggcgggat caaggccggc ggcggccgcc gcccgcaggt
     1741 actcgactcc ggtcgccacg gcgatcccga gctcccagcc agcggtggct cccgcctcca
     1801 catagatgcc ggtgtctacc gcaagcgccc gggccttcgg ccagtcgcgc gcggtctctg
     1861 ccgccagccc tccggccgcg gcaagcgcat cctcgggcga gcagggcagc tcgccggtgc
     1921 gggcgatctc gccgagcggg tcgtagcgga aggagccgat cgc
//
LOCUS       DAOQFI010000400         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35660_length_1962_cov_1.758259, whole genome shotgun sequence.
ACCESSION   DAOQFI010000400 DAOQFI010000000
VERSION     DAOQFI010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35660_length_1962_cov_1.758259"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLE93_07340"
     CDS             complement(<1..371)
                     /locus_tag="PLE93_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86899.1"
                     /translation="MVKVAGMGVLSAMAIGLLMMIFHQASSTFNGTQDPVRNEAQKVL
                     QRAPGAAINGMKETTDAISGNPTTNAIASQSDAYRGTKTSVDTTSTIMKWLGIPWAPR
                     DFLAALAGLLAVLGLVSGGFMG"
     gene            complement(365..1060)
                     /locus_tag="PLE93_07345"
     CDS             complement(365..1060)
                     /locus_tag="PLE93_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86900.1"
                     /translation="MSVLKKARRIRSRLLINAIFQIIAAAGLAAAALSVRSAPMILLV
                     TFALAVGVGLLAKRTLDRRAKYGPQDKAERVLLGLCPELEAHGFGIARRGRLPEGCRD
                     FLVVYTEAGDLAFVIALAGMWPTRASVEGPQGVATELTSYGIPHVPVVLAAFMDGLLE
                     QEVFGVLAVTPQRLIGALEDVVEEFHAARNASLKAPPIPYSQIQHKQADPDFENAFGE
                     GTEQTYREEAETW"
     gene            complement(1057..1545)
                     /locus_tag="PLE93_07350"
     CDS             complement(1057..1545)
                     /locus_tag="PLE93_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNH86901.1"
                     /translation="MALATLGVFVVALSAGVVVPTGLEVPARDSSISGLLTILRSNLV
                     LLAVIGACAGLQTFARREVEEGSQPWIRRITDMVVAVFVTLNVAAAGFVIGQLGVDAV
                     IRIVPHGILEVPAFTLAVWGYLLARKGRLDPGSARRIYITATLLLLVAAPIETFITGS
                     VK"
     gene            complement(1635..>1962)
                     /locus_tag="PLE93_07355"
     CDS             complement(1635..>1962)
                     /locus_tag="PLE93_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86902.1"
                     /translation="QAAEQQVASLATHIVWVCGGTLTSARKARAKITSLGFTGSAMSI
                     LYVRGETDSVPARSAQEELGKAADESGLAIVVSPPVGDFVSQGLVPTASRAMKPLSAA
                     LERILG"
BASE COUNT          380 a          616 c          607 g          359 t
ORIGIN      
        1 cccatgaaac ccccggaaac caaacccagc actgccagca aacccgccaa ggccgccaag
       61 aagtccctcg gcgcccaggg aatcccgagc catttcatga tcgtggaggt ggtgtcaacc
      121 gaggtcttgg tgccccggta ggcatctgac tgggaggcga tcgcgttggt ggtcgggttg
      181 ccggagatcg cgtcggttgt ttccttcatc ccgttgatgg ccgccccggg tgcccgctgg
      241 agaaccttct gcgcttcgtt tcggaccggg tcctgggtgc cgttaaaggt cgacgatgcc
      301 tgatggaaga tcatcatcaa cagcccgatc gccattgcgc tcaggactcc catacctgcg
      361 actttcacca tgtttctgcc tcctcccggt aggtctgctc ggtgccttca ccgaacgcgt
      421 tttcgaagtc cggatctgcc tgcttgtgct ggatttgcga gtaagggatc ggtggggctt
      481 tcagcgaggc gttgcgggcc gcatggaact cctctacaac gtcctcgagg gcaccgatca
      541 ggcgttgcgg ggtgaccgcg agaaccccga agacttcctg ttcgagcagg ccgtccatga
      601 aagctgcgag cactaccggc acatggggaa tgccgtagct ggtcagttcc gttgcgacgc
      661 cctggggtcc ttcgacgctg gcgcgggttg gccacattcc cgccagagcg atcacgaaag
      721 cgagatcccc cgcctccgtg taaacgacca ggaagtcccg gcagccttcg ggaagccggc
      781 cgcggcgggc gatcccgaaa ccgtgtgcct ccagctccgg gcaaagaccc aacaggaccc
      841 gttcggcctt gtcctgtggc ccgtacttgg cgcgcctgtc gagggtgcgt ttggcgagaa
      901 gaccaacccc gaccgcaagt gcgaatgtga ccagcagaat catcggtgcc gaacggacac
      961 tcaacgctgc ggcggccagg ccagccgcgg cgatgatctg gaagatcgcg ttgatcagca
     1021 ggcgggaacg aatccgcctg gctttcttca ggacactcat ttcacactcc ctgtgatgaa
     1081 ggtttcgatt ggtgcggcaa ccagaagaag cagcgttgcc gtgatgtaga tccgccgggc
     1141 actacccgga tcaaggcggc ctttgcgggc gaggagatag ccccaaacgg ccagggtgaa
     1201 cgcgggaact tcaaggattc cgtggggaac gatccggatg accgcatcca ctcccagctg
     1261 cccgatcacg aagcccgctg cggcgacgtt gagggtgacg aaaacagcga caaccatgtc
     1321 ggtgatgcgg cgaatccatg gctggcttcc ttcctcgact tcccggcgag caaatgtttg
     1381 aagtccggcg catgccccga tcacggccag caacacaagg tttgaccgaa ggatcgtgag
     1441 caaccccgag atagagctgt cccttgcggg aacttccaat ccggtcggga ccacaacccc
     1501 tgcagaaagc gcgaccacga agaccccgag agttgcgaga gccacccgcc gtccccccga
     1561 cgcccggtcg tttgagccgg aacggtgaga gataccggat gagcgcaaac ccttggttgt
     1621 tgctgccggg cccgtcaacc gagaatcctt tccagtgcgg ccgacaacgg tttcattgcc
     1681 cgggacgcgg tcgggaccag gccttgggag acaaagtcac cgaccggcgg ggaaacaacg
     1741 atcgccagtc ccgattcgtc ggctgctttg ccgagttctt cctgggctga ccttgccggc
     1801 accgaatcgg tttcccctcg gacatagaga atcgacatgg ccgaaccggt gaatccgaga
     1861 ctggtgattt tcgccctggc tttccgggcg gaggtgagcg ttccgccaca aacccagacg
     1921 atgtgggttg cgaggctcgc gacctgctgt tcggccgcct gc
//
LOCUS       DAOQFI010000401         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35811_length_1957_cov_3.235016, whole genome shotgun sequence.
ACCESSION   DAOQFI010000401 DAOQFI010000000
VERSION     DAOQFI010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35811_length_1957_cov_3.235016"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLE93_07360"
     CDS             <1..285
                     /locus_tag="PLE93_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNH86903.1"
                     /translation="DPECVARLADEMGIVQPRIVVVMGEPALETINSLALPLARPLEP
                     REGEIQAFSPACDALFTPDIDGALDEETAKRRFWRAFRTLGRWHDDLPPY"
     gene            437..832
                     /locus_tag="PLE93_07365"
     CDS             437..832
                     /locus_tag="PLE93_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86904.1"
                     /translation="MGTVEQTVIGPREGHANGKALAAMILGVFAIANVVVLALFMALS
                     AETLDDSGAWWMLLPFFGLTAGLIAAFLGSLGWIDVRRGITDRRLFEAQLGAILGGVA
                     AGLILLAITVLLVMALFLAFSFAGEGGVD"
     gene            complement(853..1578)
                     /locus_tag="PLE93_07370"
     CDS             complement(853..1578)
                     /locus_tag="PLE93_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNH86905.1"
                     /translation="MPDREKISRYAKATAKAGKGGELADLLLEAAEVNRASEGCLQYE
                     VHQSVFDPDTIWVTEAWVDQDAVKASLEDEENKTLIDRARPLIEDMEVIELLPLGGIE
                     ARLDDPRAATPKPGYKLVNVAEVNDGAKDHGLGEIQEARFAIGDLEASQVGVAWFRMH
                     PNQRQPFGHVHDRAEEVYFVVSGSGRVKLDDDIVELREGDLIRVAPEIIRCFEAGPDG
                     MVYYAAGQHMSGDGDVKPGWWSD"
     gene            complement(1623..>1957)
                     /locus_tag="PLE93_07375"
     CDS             complement(1623..>1957)
                     /locus_tag="PLE93_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNH86906.1"
                     /translation="VVMIANLLIMVIIGGYETFVSRIKVENHPDQPEWLSHVNANVLK
                     VKLAMAIVGISSIHLLKTFIEYGTEETGLTSTEVMWQTIIHFTFVLSAIGLAYIDRYL
                     SPTKDDGL"
BASE COUNT          306 a          682 c          606 g          363 t
ORIGIN      
        1 gacccggaat gcgtggcccg cctggccgac gagatgggca tcgtccagcc ccgcatcgtc
       61 gtcgtgatgg gtgagcccgc cctggagacg atcaacagcc ttgccctgcc gctggcccgt
      121 ccgctcgagc cccgcgaagg cgagatccag gccttcagcc ccgcctgcga cgccctcttt
      181 accccggaca tcgacggtgc cctcgacgaa gaaaccgcca agcgccgctt ctggcgagcc
      241 ttccgcaccc tcggccgctg gcacgacgac ctccccccgt actaacccgc ccggcaccag
      301 cacctgccgg aaaccgcccc gccttcaccg ccccgccggc ccgctccggc cccgcgagat
      361 tccactttgc gccacatacc ggcacaaacc gggcactcgg tgacttcttt aggggatgtc
      421 ggactaaaat tccggcatgg gaaccgtcga acaaacggtg atcggtcccc gcgagggcca
      481 tgcgaacggc aaggcgctcg cggcgatgat cctcggcgtc ttcgcgatcg ccaacgtggt
      541 cgtcctggcc ctcttcatgg ccctgagcgc cgagacgctt gacgacagcg gcgcctggtg
      601 gatgctgctc ccgttcttcg gcctgaccgc cggactgatc gcggccttcc tcggatcgct
      661 cggctggatc gacgtccgcc gtggcatcac cgaccgccga ctcttcgaag cccagctcgg
      721 cgcgatcctc ggtggcgtcg cggccggact gatcctgctc gcgatcaccg ttctcctggt
      781 gatggccctc ttcctcgcct tcagcttcgc cggcgagggc ggggttgact gatcaccccg
      841 gccggggcag gttcagtcgc tccaccagcc gggcttgacg tcgccgtcgc ctgacatgtg
      901 ctggccggcg gcgtagtaga ccatcccgtc gggtccggct tcgaagcagc ggatgatttc
      961 cggagcgacc cggatcaggt cgccttcccg gagctcgacg atgtcatcgt ccagcttgac
     1021 ccggcccgag ccggaaacca cgaagtagac ctcctcggcc cggtcgtgga cgtggccgaa
     1081 gggctggcgc tggttcggat gcatgcggaa ccaggccacg cccacctggc ttgcttcgag
     1141 gtcaccgatc gcgaagcggg cctcctggat ctcgccgaga ccgtggtctt ttgccccgtc
     1201 gtttacttcc gcgacgttga ccagcttgta tcccggcttc ggggtggcgg cacgggggtc
     1261 gtccagcctg gcctcgattc cgccgagcgg cagcagctcg atcacttcca tgtcctcgat
     1321 cagggggcgc gcccggtcga tcagggtctt gttttcctca tcctccagcg aggccttcac
     1381 cgcatcctgg tcaacccagg cctcggtcac ccagatcgtg tccgggtcga agacggactg
     1441 gtgcacctcg tactggaggc agccttcgga agcgcggttg acctcggctg cctcaagcag
     1501 caggtcggcg agttcacctc ccttgccggc tttcgcggtt gccttggcgt agcgggagat
     1561 cttctctcgg tcgggcatgt ccggactcta cccgcgaatc gcggcgacca agccgcgacc
     1621 gctcagagcc cgtcgtcctt ggtcggcgag aggtaacggt cgatgtaggc gagaccgatc
     1681 gccgaaagga cgaaggtgaa atggatgatg gtctgccaca tcacctcggt ggaggtcagg
     1741 cccgtctctt cggttccgta ctcgatgaag gtcttgagca ggtgaatcga ggagatgccg
     1801 acgattgcca tcgccagctt gaccttgagc acgttggcat tcacatgcga aagccactcc
     1861 ggctggtcgg ggtggttctc gaccttgatc cgggaaacga aagtctcgta gccgccgatg
     1921 atcaccatga tcagcaggtt ggcgatcatc accacgt
//
LOCUS       DAOQFI010000402         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35821_length_1957_cov_2.739748, whole genome shotgun sequence.
ACCESSION   DAOQFI010000402 DAOQFI010000000
VERSION     DAOQFI010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35821_length_1957_cov_2.739748"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLE93_07380"
     CDS             <1..360
                     /locus_tag="PLE93_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNH86907.1"
                     /translation="GNSGNGKGGNAAKSQNAKPAQIRVAVLNGTGGTESGLANTYATA
                     LEDDGYQIGVVTDATDTFTESVVMFASGHQAEARKVGKAVGIDNTAPMDAAISGLSSG
                     ADVTVVIGTDHGPLPGE"
     gene            361..1044
                     /locus_tag="PLE93_07385"
     CDS             361..1044
                     /locus_tag="PLE93_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86908.1"
                     /translation="MDNPAGPRGPGTTRLGWLAPLTIGLLLVATVAAFGWSQRLKREP
                     LVIDRVEYRVLGYTPNGESPTVFSPNGDCRRDRMSIHFRTTRSDTADVEIIGLGGRTV
                     RTLARDRFFKRYREHTLIWNGRKDDGGIAFTSKYRVRVTMHGEDRVLYLPGFIRLHRY
                     APRGPSGCPKPLKWKYSVKEPGKPARPLTRAEVAKMTRPLTEAEVAKIKAAAKKALGT
                     GGTTGGQGQ"
     gene            1041..>1957
                     /locus_tag="PLE93_07390"
     CDS             1041..>1957
                     /locus_tag="PLE93_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86909.1"
                     /translation="MIEALAALVAAAALAVAILDQPSMRRSIAMAVAGIMVPVLIAGD
                     QWHSGPITELRNSPAMLVLALLCALAVVALIAWVVDRWPVMLPVLVLVALPFRIPLNV
                     GGEDANLLVPLYLVMGGGVLASLIRARRNDLLPALPTVAPAGLARWLKPLLAAAVVLY
                     ALGLFYTDDRSTGLQNLCFFLIPFGVIFALMAEVEWTRDRLRILLGVVVGLALVCALV
                     GFVEYATRDLLWNQVVIRSNDFHVYFRVNSLFWDPNVYGRYLALAITMVAAALLWAKP
                     SRESVILAVVAAVLWLALMTTYSQSSFIAL"
BASE COUNT          300 a          688 c          642 g          327 t
ORIGIN      
        1 ggcaactccg gcaacggcaa aggcggcaac gcggccaagt cgcagaacgc caaacccgcc
       61 cagatccggg tcgccgtcct caacggcacc ggtggcaccg aatccgggct cgccaacact
      121 tacgccaccg ccctggagga tgacgggtac cagatcggtg tggtgacgga cgcaaccgac
      181 accttcaccg agagtgtcgt catgttcgcg agcggccatc aggcagaagc caggaaggtc
      241 ggcaaggcgg tcgggattga caacacggcc ccgatggacg ccgccatctc gggactttct
      301 tccggtgccg acgtgaccgt ggtgatcggc accgatcacg gccccctgcc cggggagtaa
      361 atggataacc ccgccggccc ccggggccca ggcacgactc gcctgggatg gctggcgcca
      421 ctcacgatcg gcctgcttct ggtggcgacg gtggccgcct tcggctggtc gcagcggctc
      481 aagcgtgagc cgctggtgat cgaccgggtc gagtaccggg tgctcgggta cacgccgaac
      541 ggtgaatcgc cgaccgtctt cagccccaac ggcgattgcc ggcgggaccg gatgtcgatc
      601 cacttccgca cgaccaggtc ggacaccgcc gacgtcgaga tcatcggcct cggcggcagg
      661 accgtccgca ccctggcccg cgaccgcttc ttcaagcgct accgcgagca cacgctgatc
      721 tggaacggtc gcaaggacga cggcgggatc gcattcacca gcaagtaccg ggtccgggta
      781 accatgcacg gcgaggaccg ggtcctctac ctgcccggct tcatccgcct ccacaggtac
      841 gcgccccgcg gaccctccgg ttgcccgaag ccgctcaagt ggaagtactc ggtcaaggaa
      901 ccgggcaaac cggcgagacc cctgacgagg gccgaggtcg cgaagatgac cagacccctg
      961 accgaggccg aggtggcgaa gattaaagcc gcggcgaaga aggcgctggg caccggcggc
     1021 accaccggag ggcagggcca gtgatcgagg cgctcgcagc cctcgtcgcc gcggccgcgc
     1081 tggcggtcgc gatcctcgac cagccttcga tgcgtcgctc gatcgcgatg gcggtcgccg
     1141 gaatcatggt cccggtcctg atcgccggtg accagtggca ttccggcccg atcaccgaac
     1201 ttcgaaactc accggcgatg ctggtcctgg ccctgctctg cgccctggcc gtggtcgccc
     1261 tgatcgcctg ggtggtcgat cgctggccgg tcatgctccc ggtgctggtc ctggtcgcgc
     1321 tgcccttccg gatcccgctg aacgtcggcg gggaggatgc gaacctgctg gtcccgctct
     1381 atctggtgat gggcggcggg gtgctggctt cgctcatccg ggcgcgtcgg aacgacctgc
     1441 tgcccgcgtt gccgaccgtt gccccggccg gtctggcccg ctggctcaaa cccctgctcg
     1501 ccgccgcggt ggtgctctac gccctcggcc tcttctacac ggatgaccgt tcgaccggcc
     1561 tccagaacct ctgtttcttc ctgattccct tcggggtgat cttcgccctg atggccgagg
     1621 tcgagtggac cagggaccgc ctgcggatcc tgctcggcgt ggtcgtcggc ctggccctgg
     1681 tctgcgccct ggtcggcttc gtggaatacg cgacgcggga cctgctctgg aaccaggtcg
     1741 tgatccgatc gaatgacttc cacgtctact tccgggtcaa ctcgctgttc tgggatccga
     1801 acgtctacgg ccgctacctg gcgctggcga tcaccatggt cgccgcggca ctgctctggg
     1861 cgaagccgag ccgcgagtcg gtgatcctcg cggtggtcgc cgccgtcctc tggctcgccc
     1921 tgatgaccac ctactcgcag tcgagcttca tcgccct
//
LOCUS       DAOQFI010000403         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35879_length_1955_cov_3.001579, whole genome shotgun sequence.
ACCESSION   DAOQFI010000403 DAOQFI010000000
VERSION     DAOQFI010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35879_length_1955_cov_3.001579"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PLE93_07395"
     CDS             <1..1406
                     /locus_tag="PLE93_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86910.1"
                     /translation="AMAVTVIVLAWGLTFPIGGIVQNGAIRFGLLPVSLVFFRIASIR
                     LAGGGRAGLARVSGWLAWAVVGLSAIWALEALLYTTATLTGLVLAEAMTRPPGSRRRW
                     ILGRVVRALIAWAAFHLAFAIYILIAAGQLPDWGMYISYLRDFLVGKVGDLTYDFEPW
                     SPGLLVGFGYLTSATATALAIWRRRDWFDANQVAFIALAGLSLYGLIQFSYFDNRSLA
                     TILPFLCFPLLLSATIWLDLIQRDPGAGPALRRGAVAVALSLAAVTVSTVWPDARERS
                     TDTALAYALPGGRSLADGLERLGDMPPLTGGADEGERLLRKYMPGESESAVATRYDLD
                     LNILIRADRANRLGIADANEASWVPGPHVPVMEEAVDSLASGDRMLVDQGALRQFSLL
                     QQDPEALPDEFTTDDGQTVIQLMALARIGRRFDLKTVARGSDGLEVVELVPRGSGDEL
                     AGGKDPRPVQDQAESGP"
     gene            complement(1338..>1955)
                     /locus_tag="PLE93_07400"
     CDS             complement(1338..>1955)
                     /locus_tag="PLE93_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH86911.1"
                     /translation="TDETVDLARQLEINVVWHPHQVGYGGNQKTCYLEALQRGADVVV
                     MLHPDGQYEPGLIPSMIEPIVADQADLVLGSRLLVEGWALSAGMPRWKFIANRFLTTI
                     QNRIMGTRLSEAHTGYRAYSRELLLTVPFLRNSLDFVFDSELLYQASYFGFRIREVPA
                     RCRYFDEMSSVGFRTGVVYGLKTLWAGFRLILHRARILPSRKFIA"
BASE COUNT          292 a          621 c          696 g          346 t
ORIGIN      
        1 gagcgatggc ggtgaccgtg atcgtgctcg cctggggtct caccttcccg atcggcggga
       61 tcgtccagaa cggggcgatc cggttcggcc tccttcccgt gtcgctggtc ttcttccgga
      121 tcgcctcgat ccggctggcc ggcggggggc gagccggtct ggcccgggtc tcgggctggc
      181 ttgcctgggc ggtggtcggg ctttcggcga tctgggccct ggaggcgctg ctttacacga
      241 ccgccaccct gaccgggctg gttctggccg aggcgatgac ccggccgccg gggagccgtc
      301 gccgctggat tctggggaga gtcgtccgcg ccctgatcgc ctgggccgcc tttcacctcg
      361 ccttcgcgat ctacatcctg atcgcggccg gccagttgcc ggactggggc atgtacatct
      421 cgtatctgcg ggacttcctg gtcggcaagg tcggtgattt gacctatgac ttcgaaccct
      481 ggtcaccggg gttgctggtc ggcttcggct acctgacctc ggcgaccgcg acggcgcttg
      541 cgatctggcg ccgccgcgac tggtttgacg ccaaccaggt tgccttcatc gcgctggccg
      601 gcctcagcct ctacggcctg atccagttca gctacttcga caatcgttcg ctggcgacga
      661 tcctcccctt cctctgcttt ccgctgcttc tctcggcgac gatctggctg gatctgatcc
      721 agcgcgatcc cggagccggg ccggcgcttc ggcggggtgc ggtcgcggtt gccttgagcc
      781 tcgcggcggt caccgtgtca accgtctggc cggacgccag ggagcggtcc accgataccg
      841 ctctcgccta tgccctgccg ggaggccgca gcctcgccga cggcctggag cggctcggtg
      901 acatgccgcc ccttaccggc ggcgccgatg aaggagaacg gctgctccgg aagtacatgc
      961 ccggcgagag cgagagtgcg gtggcgaccc gctacgacct ggacctgaac atcctgatcc
     1021 gggccgatcg ggcaaaccgg ctgggcatcg ccgacgcaaa cgaggcgagc tgggtgccgg
     1081 gaccgcatgt tccggtcatg gaggaagcgg tcgactcgct tgcgtccgga gaccggatgc
     1141 tggtcgacca gggcgcactc cgccagttca gcctgcttca gcaggacccg gaagcgttgc
     1201 cggacgagtt caccaccgat gacggccaga ccgtgatcca gctgatggca ttggcgcgga
     1261 tcggccggcg cttcgatttg aagacggttg ccaggggcag cgacggactc gaggtggtcg
     1321 agctggtgcc ccggggttca ggcgatgaac ttgcgggagg gaaggatccg cgcccggtgc
     1381 aggatcaggc ggaatccggc ccatagggtc ttcagcccgt agaccacccc ggtcctgaaa
     1441 ccgaccgagg acatctcgtc gaaatagcgg cagcgggccg gaacctcgcg gatgcggaac
     1501 ccgaagtagg aggcctggta aagcagctcg gaatcgaaca cgaagtcaag cgagttgcgc
     1561 aggaacggaa ccgtgagcag cagctcgcgg gagtaggcgc ggtaaccggt gtgggcctcg
     1621 gagaggcggg tgcccatgat ccggttctgg atcgtggtga ggaagcggtt ggcgatgaac
     1681 ttccagcgcg gcatgccggc ggaaagggcc cagccctcga cgagcaggcg cgaaccgagc
     1741 acgaggtcgg cctggtcggc gacgatcggt tcgatcatgc tggggatgag ccccggttcg
     1801 tactggccgt cggggtggag catcacgacc acgtcggcgc cacgctgcag cgcctcgagg
     1861 tagcaggtct tctggttgcc tccgtagccg acctgatggg gatgccagac cacgttgatc
     1921 tcgagctggc gggccaggtc gacggtctcg tcggt
//
LOCUS       DAOQFI010000404         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35944_length_1953_cov_4.109062, whole genome shotgun sequence.
ACCESSION   DAOQFI010000404 DAOQFI010000000
VERSION     DAOQFI010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35944_length_1953_cov_4.109062"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PLE93_07405"
     CDS             <1..1116
                     /locus_tag="PLE93_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNH86912.1"
                     /translation="VDFQGFADVIDAIHCVYVDVDRHYFNDNSQGGEEYATIDIEAGY
                     QKLCGSDALDFARYRHTDSDLVRAARQQDLMGEVRNRLSFGELLSQRDELLKAFVDNT
                     TTDIGETGDSKDVFSLLKLLLDSRGAAVTEVTFPSTFTMRDGVSYVEATPDEIQAAVD
                     KFLGFEDQPGPVGTLDQGDGANNPARKEAQQKQQKKAARERQKALDESTTHVPSKDED
                     QLVDASSSGLSVAQELDSEITRTDFPVYYPKRLPSGALYADGESRVYHTRDPDKNVYG
                     AYRMVMEVQLDDGLHYFGLQGLRGWTDPPILDAPHDEVEMDDRTFQVYPEGDRIKLIA
                     WTDGDNVYWISNSLLLGLDNEQMLGMARSTRAFTPDK"
     gene            1122..>1953
                     /locus_tag="PLE93_07410"
     CDS             1122..>1953
                     /locus_tag="PLE93_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNH86913.1"
                     /translation="MNSEEKTPVGVIGVGWVGLVTAACFAELGHPVIARDVVQEKVDA
                     LSRGEITIHEPGLAELLERNRDRLTFTTDMDELLNGARLLFVCVDTPPTYSGDADLSR
                     VRSVVEDLSADGDHVLIMKSTVPAGTGASIRRELPGLAYVSCPEFLKEGTAVEDFMHP
                     DRVVVGSEPEDSAAAEEVADLYRPLGGEFVMTDTSSAEMIKLASNAYLATRISFINEI
                     ANVCEEVGADVSEVARGMGLDERIGPHFLRPGIGFGGSCFPKDVNALKLLAGNSGYHF
                     Q"
BASE COUNT          374 a          645 c          612 g          322 t
ORIGIN      
        1 gtcgacttcc agggattcgc cgacgtgatc gacgcgatcc attgcgtcta cgtcgacgtt
       61 gaccgccatt acttcaacga caacagccag ggcggcgagg agtacgcgac gatcgacatc
      121 gaggccggct accagaagct ttgtggctcc gatgcgctcg acttcgcccg ttaccggcac
      181 accgactccg acctggtccg ggcggcccgc cagcaggacc tgatgggcga ggtgcgcaac
      241 cggctcagct tcggtgagct gctgagccag cgcgacgagc tgctcaaggc cttcgtcgac
      301 aacacgacaa cggacatcgg cgagaccggt gacagcaagg atgtcttttc gctgctgaag
      361 ctcctgctcg attcacgggg cgccgccgtg acagaggtga ccttcccctc gacgttcacg
      421 atgcgggacg gcgtctccta tgtcgaagcc accccggacg aaattcaggc tgcggttgac
      481 aagttcctcg gtttcgagga tcagcccgga ccggtcggca ctctcgacca gggcgacggg
      541 gccaataacc cagcccgcaa ggaagcgcag caaaagcaac agaagaaggc cgccagggag
      601 cgtcagaaag cgctggacga gtcaaccacg catgtcccga gcaaggatga ggaccagctg
      661 gttgacgcct ccagcagcgg gctctcggtg gcacaggagc tcgacagcga gatcacccgc
      721 accgacttcc cggtctacta cccgaagcgg ctgccctccg gcgcgctcta cgccgacggc
      781 gagagccggg tctaccacac ccgtgatccg gacaagaacg tctatggcgc ctaccgcatg
      841 gtgatggagg tccagctcga cgacggtctg cattacttcg gcctgcaggg cctgcgtggc
      901 tggaccgacc ctcccattct cgatgcgcct cacgacgagg tcgagatgga cgaccggacc
      961 ttccaggttt acccggaagg cgaccgcatc aagttgatcg cctggaccga cggcgacaac
     1021 gtgtactgga tctccaattc gctgctcctc ggtctcgaca acgagcagat gcttggcatg
     1081 gcccggtcga cccgggcatt cacccccgac aagtgagtga aatgaactca gaagaaaaga
     1141 cgccggttgg cgtcatcggg gtcggctggg tcggcctcgt caccgctgca tgcttcgctg
     1201 aactcggcca cccggtaatc gcccgggacg tggttcagga aaaggtcgac gcgctaagcc
     1261 gcggcgagat cacgatccac gagcccggcc tggccgagct cctggagagg aaccgcgacc
     1321 gcctgacctt cacgaccgac atggacgaac tgctgaacgg cgcccgcctc ctcttcgtct
     1381 gtgtggacac tccgccgacc tactccggtg acgccgacct ttcccgggtt cgctcggtgg
     1441 tcgaggacct cagcgccgac ggggatcacg tgctgatcat gaagagcacg gtgccggccg
     1501 gcaccggggc ctcgatccgg cgcgagcttc cggggctggc ctacgtgtcc tgcccggagt
     1561 tcctgaagga aggcacggcg gtcgaggact tcatgcaccc tgaccgggtc gtggtcggat
     1621 ccgagccgga ggacagcgcc gcagcggaag aagtcgccga cctctaccgc cccctcggtg
     1681 gcgagttcgt gatgaccgac acctcgagcg ccgagatgat caagctcgcc tcgaatgcct
     1741 acctggcgac ccgcatctcc ttcatcaacg agatcgcgaa cgtctgcgaa gaggtcggtg
     1801 ccgacgtcag cgaggtcgca cgggggatgg gtctggacga gcggatcggc ccccacttct
     1861 tgcgtcccgg gatcggcttc ggcggttcct gctttcccaa ggacgtcaat gccctgaaat
     1921 tactggccgg taactcgggc taccacttcc agc
//
LOCUS       DAOQFI010000405         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_35980_length_1952_cov_4.319979, whole genome shotgun sequence.
ACCESSION   DAOQFI010000405 DAOQFI010000000
VERSION     DAOQFI010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35980_length_1952_cov_4.319979"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLE93_07415"
     CDS             <1..729
                     /locus_tag="PLE93_07415"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNH86914.1"
                     /translation="KTGAFQHLDTSPMLMAPVIGGPLLSAIGGLTGSRKLAAAGLLLS
                     AGSTASMVDIGLRKVVPGANDNGTAVIALLELARRFLADPPEGLRLILLSAGSEESFS
                     EGMKAFGERHFPNLPVDSTFFINMDTIGSPYLTVLRGEGFLKMFEYPPEALILADRAA
                     EEEGIELMPNLRIHNGTDGLEALAAGYPVVSICSCTEHKQPANYHWPNDVSENVDFDT
                     VESGIRLAEAMARRLSRDWVSLPG"
     gene            738..>1952
                     /locus_tag="PLE93_07420"
     CDS             738..>1952
                     /locus_tag="PLE93_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH86915.1"
                     /translation="MAPALTLPLRNSVYRRLFSAQIVGLLGTGISTVALGLLAFSMGG
                     GNAGQILGVVLALKVATYVLVSPAIAAFAAGVSRKKLLVTMDVVRALAIGSIAFVGEI
                     WQVYVLIVIVSAASAGFTPAFQALIPDIFTDVDTYTEALSLSRLAYELEALVSPLIAG
                     LLLAVVGYTELFTLNSLAFLTSAGLVLTTAVPAVRDDALAARKLSQVSQGIRQFLRTP
                     RLQGLFLLDWAAAAAGAMVLINTVIYVKGQLGFGDSDVAWALGAFGAGSIVIALLLPT
                     LLRRFTDRSLMLLGGLILAASMFLFVAVGSLTTLMACWALAGIGTSAVLTPSGRLIQR
                     SGDSAGRPALFAAQFSLSHFCWLFTYLIAGFAGEALGLSEVALILGAIAAGASVLAAM
                     VWRPEPPARQASS"
BASE COUNT          290 a          638 c          593 g          431 t
ORIGIN      
        1 aagaccggcg ccttccagca cctcgacact tcaccgatgc tgatggcccc ggtgatcggt
       61 ggcccgctgc tcagcgcgat cggcggtctg accgggagcc ggaagctggc agcggccggg
      121 ctcctgcttt cggccggatc gaccgcctcg atggtcgaca tcggcctgag gaaggtcgtt
      181 ccgggggcca acgacaacgg cacggccgtg atcgccctgc tcgaactcgc ccgccggttt
      241 ctcgcagacc ctcccgaggg tctccgcctg atccttctct cggcgggctc cgaggagtca
      301 ttcagcgagg gaatgaaagc cttcggggaa cgacactttc cgaacctgcc ggtcgactca
      361 acctttttca tcaacatgga cacgatcggc tccccgtact tgacggtgct gcgcggggag
      421 ggtttcctga aaatgttcga gtacccgcct gaagccctga tcctggccga tcgggccgcc
      481 gaagaggaag gcatcgagtt gatgccgaac ctgaggatcc acaacgggac cgatggtctg
      541 gaggcactag cggccggtta cccggtggtc tcgatctgtt cgtgtaccga gcacaagcag
      601 ccggccaatt accactggcc gaatgacgtt tcagagaacg tcgacttcga caccgtcgag
      661 tccggcatcc gccttgccga agctatggcc cgccgcctct cccgggactg ggtcagcctc
      721 ccgggctgaa cgcttccgtg gcgcccgcgc tgacacttcc tctccggaac agtgtctatc
      781 gccgtctgtt ttccgcgcag atcgtcgggc tgcttggaac cggaatatcg acggtcgctc
      841 tgggcctgct ggcattcagc atgggaggag gcaacgcagg tcagattctt ggagtggtac
      901 tcgccctgaa agttgccacc tatgttctcg tttccccggc aatagccgca ttcgccgccg
      961 gtgtctcacg aaagaagttg cttgtcacga tggacgtggt ccgggcgctg gccatcggtt
     1021 cgatcgcttt cgtcggtgag atctggcagg tctacgtcct gatagtgatc gtcagtgcgg
     1081 cctcggcggg gttcaccccg gcattccagg ccttgatccc cgacatcttc accgacgtgg
     1141 acacctatac cgaggcacta tccctcagtc gcctggccta cgagctggaa gctctcgtca
     1201 gccctttgat tgccgggttg cttctggctg tggtcggcta cacggagctg ttcaccctga
     1261 attccctggc cttcctgacc tccgcgggac tcgtgttgac caccgctgtc ccggctgtcc
     1321 gggatgacgc cctggccgcc agaaagttgt ctcaggtttc ccagggcatt cgccagtttc
     1381 tgcgtacccc acggctccag ggtctctttc tcctcgactg ggccgctgcg gccgccggcg
     1441 ccatggtcct catcaacacg gtgatctacg tcaagggaca gcttggattc ggtgactccg
     1501 acgtcgcctg ggctctgggg gcgtttggtg cgggatcgat cgttattgcc ctgctcctgc
     1561 cgactctgct tcgtcgcttc accgatcggt cgctgatgct ccttggtggc ctgatcctgg
     1621 cggcttcgat gttcctgttt gtcgcagttg gatcgctgac gacactgatg gcctgctggg
     1681 cactcgctgg aatcggaact tccgcggtcc tgactccttc aggccgtctg atccagagaa
     1741 gcggcgactc ggccggtcgg ccagccctct tcgccgccca gttctcgctt tcacacttct
     1801 gctggctctt tacgtacttg atcgccggct tcgcaggaga agctctgggg ctttccgagg
     1861 ttgccctgat tctcggcgcg atcgccgccg gcgcgagtgt cctggcagcg atggtgtggc
     1921 ggcccgagcc gcctgcgcgt caggcctcgt ct
//
LOCUS       DAOQFI010000406         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_36131_length_1948_cov_3.238246, whole genome shotgun sequence.
ACCESSION   DAOQFI010000406 DAOQFI010000000
VERSION     DAOQFI010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36131_length_1948_cov_3.238246"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1251)
                     /locus_tag="PLE93_07425"
     CDS             complement(151..1251)
                     /locus_tag="PLE93_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNH86916.1"
                     /translation="MRAAVLEEFGKPLVVQEVDLAEPKEGEVLVRLEACGVCHTDMYT
                     ASGADPSGYAPCVLGHEGGGIVEKVGEGVTSLAPGDKVVTLFSPQCRECEHCLDPRTN
                     LCMAIRAEQNLGHLPDGTTRLSRDGEEIRHFMGTSTFAEYTVMPEIALAKINPEAPLD
                     HACLFACGLSTGLGAAMFTAKVEPGTTCVVFGAGMVGLGAVAGARLMGAERIICVDLS
                     DDRLELARGQGATDLHKADENTVQWILDETGGMGADYTFEATGLVGVMQQAVEAARMA
                     WGLCTIAGVAGKGETLDIIPRWLITGRRVAGSSFGGVKGRDQEPQLVDRYLAGEIDVE
                     PFLSHRISLDEVNKGFDLMEAQDGIRSVIEFT"
     gene            1653..>1948
                     /locus_tag="PLE93_07430"
     CDS             1653..>1948
                     /locus_tag="PLE93_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86917.1"
                     /translation="MPFRRLIPLAAAVLLLAIAGCGSEGGDGSSPGGDSASGGSETEQ
                     VKVFTTEGEQFDPVQKEVPAGDLPKAATQALLDAPADPKSGKSRQRKNRLSKSR"
BASE COUNT          359 a          673 c          609 g          307 t
ORIGIN      
        1 ccgccggtct cgtcgcagac gaggtaggag ttggagagcc attcggactg ttcgactcgc
       61 tggatgatca tgcgcggtcc tttcgggtgg gatggaagga agagtgactg gttttcggcc
      121 gggagcgcgg catcggctgc gggcggggca tcaggtgaac tcgatcacgc tgcggatgcc
      181 gtcctgggct tccatcaggt caaagccctt gttgacctcg tcgaggctga tccggtggga
      241 gaggaacggt tcgacgtcga tctcgccggc gaggtagcga tcgaccagtt gcggctcctg
      301 gtcgcggccc ttgacgccgc cgaacgaaga gccggcgacc cggcgaccgg tgatcagcca
      361 gcggggaatg atgtcgagag tctcgccctt gccggccacg ccggcgatcg tgcagagccc
      421 ccatgccatg cgggccgctt cgaccgcctg ctgcatcacc cctaccaggc cggtcgcttc
      481 gaaggtgtag tcggcgccca ttccgccggt ttcgtcgagg atccactgga ccgtgttttc
      541 gtctgccttg tggaggtcgg tcgcgccctg gccccgggcc agttcgagcc ggtcatccga
      601 gaggtcgaca cagatgatcc gctctgcccc catcaggcgc gccccggcga ccgcgccgag
      661 gccgaccatg ccggctccga agacgacgca ggtcgtgccc ggttcgacct tggccgtgaa
      721 catcgccgcg cccagcccgg ttgaaaggcc gcaggcgaaa aggcaggcat ggtcaagcgg
      781 agcttcggga ttgatcttgg ccagggcgat ctccggcatc accgtgtact cggcaaaggt
      841 cgaggtgccc atgaagtggc ggatttcctc gccgtcgcgg gaaaggcggg tggtgccgtc
      901 gggcaggtgg ccgaggttct gctcggcccg gatcgccatg cagaggttgg tccggggatc
      961 gaggcagtgt tcgcattcgc ggcactgcgg cgagaacagg gtcacgacct tgtcgccggg
     1021 tgccagcgag gtgactcctt cgccaacttt ctcgacgatc ccgccgccct cgtgaccgag
     1081 cacgcagggc gcgtacccgg acggatcggc ccccgaggcg gtgtacatgt cggtgtggca
     1141 gaccccgcag gcctccagcc ggaccagcac ctcaccctct ttcggctcgg ccagatcgac
     1201 ctcctggacc acgagcggtt tgccgaactc ctcaagaaca gcagccctca tcctcataac
     1261 ccgatcctag agagccaacc ccccaagtca aaacccaacc aaaacccaag aaccaagctt
     1321 caacctcaac caccaaccac caatcccaac caagcttcaa ccccaacccc cagccagggc
     1381 ccaccaaatt cccaccaaac caagaaaccc ctcccaccag aatccttccg ggggcgatca
     1441 cccaccgatc cagtagccga actcccccga aaaccaggaa gaagccacca accaccccgc
     1501 ccgttccggg ggcgatcacc caccatttcc gtggccagac tcccccggaa ggacgaacct
     1561 ggccagcctc ccgccgttcc gggggcgatc gcccaccgaa tccgtagcca atcgcccccg
     1621 gttccggtgg ctggcggcaa tagggtgtgg ccgtgccgtt ccgtcgcctg attccgctcg
     1681 cagctgccgt cctcttgctc gcaatcgctg gctgtggctc ggagggcggg gatggctcga
     1741 gtcccggcgg tgattcggcc tctggcggaa gcgaaaccga gcaagtcaag gtcttcacta
     1801 ccgaaggcga gcagttcgat ccggtccaga aggaagtacc cgcgggcgac ttgccgaaag
     1861 ccgcgaccca ggccctcctc gatgcgccgg cggacccgaa gagcgggaag agccgccagc
     1921 ggaagaaccg gttgtcgaag agccgcca
//
LOCUS       DAOQFI010000407         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_36359_length_1942_cov_2.945946, whole genome shotgun sequence.
ACCESSION   DAOQFI010000407 DAOQFI010000000
VERSION     DAOQFI010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36359_length_1942_cov_2.945946"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLE93_07435"
     CDS             complement(<1..596)
                     /locus_tag="PLE93_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNH86918.1"
                     /translation="MAATPQAVPRSPAEVAEALRDASYQADDSTALIAFLAQRLGKPV
                     LVEGPAGVGKTELAKALSRATGRELVRLQCYEGLDEAKALYEWNYRKQLLQIQATAST
                     QDEGATPVGMGEIFTEDFLLERPLMKAIANPDPVVLLIDEIDKTDQEFEAMLLELLSD
                     FQITIPEMGQISATTHPIVLLTSNNSRELTEALKRRCL"
     gene            complement(645..1055)
                     /locus_tag="PLE93_07440"
     CDS             complement(645..1055)
                     /locus_tag="PLE93_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86919.1"
                     /translation="MPPKPEQTEQQPGADRFWGQFSQSKAGRSSAENGSGAESAKAGS
                     EGPDGQGHGAECLEWCPICRSAELFRHTVSPELRQQAESIQHEAIGVLKAFLDAYADK
                     SGNSSPGGPSGPEPSPSDQPEEPATPRVTDIPLD"
     gene            complement(1039..>1942)
                     /locus_tag="PLE93_07445"
     CDS             complement(1039..>1942)
                     /locus_tag="PLE93_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HNH86920.1"
                     /translation="VDQVRAEELSVPPGLDELFSLLQIKRHWDKSEFDVVIVDCAPSG
                     ETLRLLGYPEVASWWVRKVFPWNRSLLSKAAPIAKALEIPMPEPELMDEVEHLIENLL
                     AMDGILRDHEHCSIRLVMNPDRMVINEARRTFTHLCLFGYLTDAVIVNRVFPDEVADS
                     YFAGWREQQATQLESVEEGFSPVPVLTARYFDREVIGEEMHERLADDLYGEIDPSGLL
                     HSGMPREVATEDGRTVIRILAPFTEKGEVKLQQAGEELMVQVGEARRTIMLPSGLARR
                     SPSKASFEDETLEIIFEDPHATQA"
BASE COUNT          320 a          615 c          634 g          373 t
ORIGIN      
        1 tagaggcagc gccgtttcag ggcctcggtc agctcgcgcg aattgttcga agtcagcagc
       61 acgatcgggt gggtcgtggc gctgatctgc cccatctccg gaatcgtgat ctggaaatcg
      121 gaaagcagct cgagcagcat cgcctcgaac tcctggtcgg tcttgtcgat ctcgtcgatc
      181 agcaggacca ccgggtccgg gttggcgatc gccttcatca gcggccgctc gagcaggaaa
      241 tcctcggtga agatctcgcc catgccgacc ggagtcgcgc cttcgtcctg ggtcgatgcg
      301 gtggcctgaa tctgcagcag ctgcttgcgg tagttccact cgtagagggc cttcgcctcg
      361 tcgagcccct cgtagcactg gagccggacc agctcccggc cggttgcccg ggaaagggcc
      421 ttggccagtt cggtcttgcc gaccccggcc gggccttcga ccagaaccgg cttgcccagc
      481 cgctgggcga ggaaagcgat cagcgcagtt gaatcatcgg cctgatacga cgcatcgcga
      541 agagcttcgg cgacttcggc gggactccgg ggaacggctt gaggggtggc tgccatgcct
      601 tggacaatac gccgaccgcg ccggaacccg ggttcatgcc ggtttcagtc gaggggaatg
      661 tcagtgaccc gcggggtcgc cggctcttcg ggctgatccg aaggtgacgg ctccgggccg
      721 gagggaccgc ccggtgagga gttgccgctc ttgtcggcgt atgcgtcgag gaatgccttc
      781 agcacgccga tcgcctcatg ctggatcgat tccgcctgct ggcgcaactc gggtgagacg
      841 gtgtggcgaa aaagctcggc cgaacggcag atcgggcacc actcgaggca ttcggcgccg
      901 tgcccctgac catccggacc ttcgcttccc gctttcgccg attctgctcc cgagccgttt
      961 tcggccgaac tccggccggc ttttgactgt gagaactggc cccagaagcg gtcggcaccg
     1021 ggttgctgtt cggtttgttc aggcttgggt ggcatgcgga tcctcgaaga tgatctccag
     1081 ggtctcgtcc tcgaatgagg ctttcgacgg gctgcgtctg gcaagcccgg acgggagcat
     1141 gatagtccgc cttgcctcgc cgacctgaac catcagctct tcgccggcct gctggagttt
     1201 cacttcgccc ttctcggtga atggcgcaag gatgcgaatt acggtccggc catcctcggt
     1261 tgcgacctcg cgcggcatgc cggagtggag caggccggaa ggatcgatct ccccgtaaag
     1321 atcgtcggcg agccgctcat gcatctcttc gccgatcact tcccggtcga agtagcgggc
     1381 ggtaagtacc ggcaccgggg agaagccttc ctcgaccgat tcgagctgcg tggcctgctg
     1441 ctcgcgccag ccggcgaaat aggaatcggc gacttcgtca gggaagacgc ggttgacgat
     1501 cacggcgtcg gtcaggtacc cgaacaggca gaggtgggtg aaggtgcgcc gcgcttcgtt
     1561 gatcaccatc cggtccggat tcatgaccag ccggatcgag caatgctcgt ggtcacggag
     1621 gatcccgtcc atcgccagca ggttctcgat caggtgctcg acctcgtcca tcagctcggg
     1681 ctcgggcatc gggatctcga gcgccttggc gatcggcgcc gccttgctca gcaggctgcg
     1741 gttccagggg aagacctttc gcacccacca gcttgcgact tccgggtagc cgagcaaccg
     1801 cagggtttcg ccgctcggag cgcaatcgac gatcaccacg tcaaattccg acttgtccca
     1861 gtggcgcttg atctgaagca gcgagaaaag ctcgtcgagt ccgggcggga cgctcaactc
     1921 ctcggcccgc acctggtcga cc
//
LOCUS       DAOQFI010000408         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_36481_length_1938_cov_4.144981, whole genome shotgun sequence.
ACCESSION   DAOQFI010000408 DAOQFI010000000
VERSION     DAOQFI010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36481_length_1938_cov_4.144981"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..959
                     /locus_tag="PLE93_07450"
     CDS             51..959
                     /locus_tag="PLE93_07450"
                     /inference="COORDINATES: protein motif:HMM:NF020604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HNH86921.1"
                     /translation="MSKPERSYLVCATPRSGSTLVCQTLIRTGVAGRPEEYFEALRSS
                     GVPRQPEEYFHGVGDRTIFDHLAERAQDFNPPPRSPLWSRTAYDRYLDWVFDVGTTDN
                     GVFGAKLMWGYVEDFVSLLRNIPEFREVPMAEILSEVFPELTFVRVVRANKIRQAVSL
                     WKAVQTATWRDETTASSSADAEDIDASYKGFVNDHRPALRFHYGAIKHLLDQLIQQEA
                     AWDAFFEHSRIKPVFILYENFASDIGPSTRNVLERLGIEAPDDLNLETRMRRQSDGIN
                     DDWARRYSDIRLGAEFDLVPTPSGRD"
     gene            979..>1938
                     /locus_tag="PLE93_07455"
     CDS             979..>1938
                     /locus_tag="PLE93_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNH86922.1"
                     /translation="MTDRPNIIYVHSHDTGRYVQPYGHHVLTPNMQLLADQGITFRRA
                     FCAAPTCSGSRAALLTGQHCHNNGMMGLAHRGWKLNDYSQTMVHPLKEGGYRTILVGE
                     QHISRDPHVIGYDEVIELSDNYAKRVAPSALEAIRRTSEPFFMSVGFFETHRDFAAPT
                     SVRDSLYSLPPENLPDTAETRSDMASFKESVRSLDQGIGALLNGLHATGLADRTLVIC
                     TTDHGLAFPKAKATLLDGGIGVMMLMRGPNGFSGGKVIDSMVSHLDIYPTVCELAGVP
                     PPDFLQGRSLMPLVSGESAALHEELFSEVTYHAAYEPQRAVRTN"
BASE COUNT          356 a          673 c          584 g          325 t
ORIGIN      
        1 ggggtactgt caccttgacc aacgaagatt cctgagtaaa ctggtcaact atgagtaagc
       61 ccgaacgctc atacctggtc tgtgcgacgc cccgaagcgg gagcaccctg gtctgccaga
      121 ccctgatcag aaccggggtc gcgggccggc ccgaggaata cttcgaggca ctcagaagca
      181 gcggggtgcc gcgtcagccg gaggagtatt tccacggggt gggggaccgg acgatcttcg
      241 atcacctcgc cgagcgggcc caggacttca atcccccgcc gcgttcgccg ctctggagcc
      301 gcaccgccta cgaccgttat ctcgactggg tcttcgatgt cggcaccacc gacaacgggg
      361 tcttcggggc aaagctgatg tggggctacg tcgaggactt cgtctcgctg ctccgcaaca
      421 tccccgaatt cagggaagta ccgatggcgg agatcctctc cgaggtcttc cccgagctga
      481 ccttcgtgcg ggtggtcagg gcaaacaaga tccgccaggc cgtttcgctc tggaaggccg
      541 tccagaccgc tacctggcgc gatgaaacca ccgcgagctc cagcgctgac gcggaagaca
      601 tcgacgcctc ctacaagggt ttcgtcaacg accaccgccc ggcgctccgc tttcactacg
      661 gggcgatcaa gcacctgctc gaccagctga tccagcagga agctgcctgg gacgcgttct
      721 ttgagcattc ccggatcaag cccgtcttca tcctctacga gaacttcgcc tcggacatcg
      781 ggccgagcac ccgcaacgtg ctcgagaggc tgggcatcga ggccccggat gacctcaatc
      841 tggagacgag gatgcggcgc cagtccgatg gcatcaacga cgactgggcc cggcgctaca
      901 gcgacatccg cctcggcgcc gagttcgacc tggtcccgac cccttccggc cgggactgag
      961 cggctccgga cttcgccaat gacggaccgc cccaacatca tctatgtgca ttcccatgac
     1021 acgggccgct atgtccagcc ctacggccat cacgtcctga ccccgaacat gcagctgctg
     1081 gccgaccagg ggatcacttt ccggcgggcc ttctgcgccg ccccgacctg ctcggggagc
     1141 cgggcggccc tcctgaccgg ccagcattgc cacaacaacg gaatgatggg gctcgcccac
     1201 cggggctgga agctgaacga ctactcgcag acgatggttc atcccctgaa ggagggcggc
     1261 taccggacca tcctggtcgg cgagcagcac atctcccgcg acccgcacgt gatcggttac
     1321 gacgaggtga tcgagctgag cgacaactac gccaagcggg ttgcccccag cgccctcgaa
     1381 gcgatccgcc ggacaagtga gcccttcttc atgtcggtcg gcttcttcga aacccatcgt
     1441 gacttcgcgg ccccgacctc ggtccgcgac tcgctttatt cgctgccgcc ggagaacctg
     1501 ccggacacgg ccgaaacgcg cagtgacatg gcctccttca aggagagcgt gcgttcgctg
     1561 gaccagggca tcggtgcact cctcaacggt ctccacgcga ccggcctggc cgaccggacc
     1621 ctggtcatct gcaccaccga ccacggcctc gccttcccca aggcgaaggc gaccctgctc
     1681 gacggcggga tcggcgtgat gatgctgatg cgaggcccga acggtttctc cggcggcaag
     1741 gtgatcgatt cgatggtctc ccacctcgac atctacccga cggtctgcga actggccggg
     1801 gtgccgcccc cggatttcct gcagggcagg tcactgatgc ccctggtcag cggggaatcc
     1861 gccgctcttc acgaagagct gttctccgag gtgacctacc acgccgccta cgagccgcaa
     1921 agggcggtgc ggaccaat
//
LOCUS       DAOQFI010000409         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_36526_length_1937_cov_3.065887, whole genome shotgun sequence.
ACCESSION   DAOQFI010000409 DAOQFI010000000
VERSION     DAOQFI010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36526_length_1937_cov_3.065887"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..859)
                     /locus_tag="PLE93_07460"
     CDS             complement(35..859)
                     /locus_tag="PLE93_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH86923.1"
                     /translation="MAAETLTPPELKTIDIAIDGEIGTMTLNRPETFNAMSPDLIEDL
                     ATAFAWLADQAPLRGLIITGAGKAFCAGGDLNWFKKGQEDPDTDIVASVRRGAELLHS
                     GIVDLQRIPYPVIAALNGPTAGAGFSLALACDIRICSEDAFLACAYGRIGVSPDGGMT
                     YFLPRVVGPSKAIEILLNDPNLSPADALEAGLVSEVVAPDQLLDRAREKADKLAKKSP
                     YYVKMAKHLVHQSLDNSITEQLQLERHGIANGMGTEDSKNAIEAFLTGGTAEFKGE"
     gene            907..>1937
                     /gene="uvrC"
                     /locus_tag="PLE93_07465"
     CDS             907..>1937
                     /gene="uvrC"
                     /locus_tag="PLE93_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNH86924.1"
                     /translation="MAESTGKQQSKEPDREARIVAERRSLPDVPGVYLFRDRDDEVIY
                     VGKAVSIRKRVASHFSGGNAGMTRLIERIEFLATESESEALIAEQSFIKRYRPRFNIK
                     LRDDKSYPYVAVSLDEDYPRVYFTRERHRPGRAYFGPFSSARRVRDTLDLLGKLFQYR
                     TCEGKEPGRRSGSPCLDYFIKRCGAPCVDYISQADYRANIDRVIDFLSGRYDQIERDL
                     DTRMKEAAAAQDFEAAAVFRDRLASVQSLFERQRISGSTVGTADLIGVATEGTDANAQ
                     VFQVRDGILAERQSFYLENPAERGIDEVTAEFIEQYYGASPAVPKRVVLGPYMSEKLA
                     EVETFLAGRR"
BASE COUNT          330 a          625 c          643 g          339 t
ORIGIN      
        1 gccaaaaagc ccgatgcccg gccccggccg gcggctactc gcctttgaat tcggcggttc
       61 cgccggtgag gaaggcctcg atcgcgttct tcgagtcttc ggtgcccatg ccgttggcga
      121 tgccgtgacg ctccagctgg agttgctcgg tgatgctgtt gtcgagactc tgatggacca
      181 ggtgcttggc catcttcacg tagtaggggg acttcttcgc cagcttgtcg gccttttcgc
      241 gggcccggtc gagcaactgg tccggtgcca cgacctctga gacgagaccg gcctcgagcg
      301 cgtcggcagg ggagagattc gggtcattga gcaggatctc gatcgccttc gaggggccga
      361 ccacccgggg caggaaatag gtcatgccgc cgtccggcga aaccccgatc cggccgtaag
      421 cacaggcgag aaaggcgtcc tcggaacaga tccggatgtc gcaggccagg gcaagcgaga
      481 agccggcccc ggcggtcggg ccgttgaggg cggcgatcac cgggtagggg atccgctgca
      541 ggtcgacgat gccggaatga agcagctcgg ccccgcgccg gaccgaggcg acgatgtcgg
      601 tgtccgggtc ttcctggccc ttcttgaacc agttgaggtc gccgccggcg cagaaggcct
      661 tgccggctcc ggtgatgatc agtccgcgca gcggggcctg gtcggcaagc caggcgaaag
      721 ccgtggccag gtcctcgatc aggtcgggac tcatagcgtt gaaggtctcc ggacggttca
      781 aggtcatcgt cccgatctcg ccgtcgatcg cgatgtcgat ggttttcagt tccgggggag
      841 tgagcgtttc agcggccata ggtggcgcca gcgtatccca acccccttgg gtcttaccct
      901 tcgcttgtgg ccgaatccac aggcaaacag caatcaaagg aaccggaccg cgaggcgcgg
      961 atcgttgccg agcgccgctc gctgccggat gtgccggggg tttacctgtt ccgcgaccgc
     1021 gacgacgaag tgatctacgt cggcaaggca gtctcgatcc gcaagcgcgt cgcctcgcat
     1081 ttcagcggcg gcaacgccgg gatgaccagg ctgatcgagc gaatcgagtt tctcgccacc
     1141 gaatcggaat ccgaggccct gattgcggaa cagagcttca tcaagcgcta ccggccccgc
     1201 ttcaacatca agttgcggga cgacaagtcc tatccctatg tcgcggtcag cctcgacgag
     1261 gattacccgc gggtctactt cacccgggag cggcaccggc caggccgggc ctacttcggt
     1321 ccgttttcct cggcccgccg ggtgcgggac acgctcgacc tgctcggcaa gctcttccag
     1381 taccggacct gcgaaggcaa agaaccgggc cgcaggtcag gcagcccctg cctcgactac
     1441 ttcatcaagc gctgtggcgc tccctgcgtc gactacataa gccaggccga ctaccgggcc
     1501 aacatcgacc gggtgatcga ctttctttcg ggccgttacg accagatcga gcgggacctc
     1561 gacacccgca tgaaggaggc ggcggccgcc caggatttcg aagctgcggc agtctttcgc
     1621 gaccggctgg cctcggtcca gtcgctgttc gaacgacagc ggatctccgg ctccacggtt
     1681 ggcaccgctg atctgatcgg cgtcgcgacc gaggggaccg atgccaacgc ccaggtcttt
     1741 caggtccgcg acgggatcct cgccgagcgc cagtccttct acctcgagaa tccggccgag
     1801 cgagggatcg acgaggtcac cgccgagttc atcgagcagt actacggggc ctcgccggcg
     1861 gtgccgaagc gggtcgtgct cggcccctac atgtcggaga agctggccga ggtcgagacc
     1921 ttcctggccg ggcggcg
//
LOCUS       DAOQFI010000410         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_36605_length_1935_cov_2.563298, whole genome shotgun sequence.
ACCESSION   DAOQFI010000410 DAOQFI010000000
VERSION     DAOQFI010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36605_length_1935_cov_2.563298"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..861
                     /locus_tag="PLE93_07470"
     CDS             97..861
                     /locus_tag="PLE93_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86925.1"
                     /translation="MFLFAAGLAVGLAGVVLKLVRDRDLMVGGKSVLPGLSLVALMTL
                     SVPVAQLLLLLLGTDLFGARNLAPAWAGLPVLIGALISLPAFSLAAVATALTVGAFLI
                     GAVRMTDPDLASTGYGGAARFIEANAGPGDVVMDSSHFTPVPLTELDTELSQDWPEYR
                     VNLPIGDPPFLPVLAQVVPPDEQIAEAFGKAAREDGKVFAVTWNPPLTDASKDGLYAP
                     EGATRGIPKGWHLTETRTWDGYRPVTVNVFEQSPSQ"
     gene            complement(913..1596)
                     /locus_tag="PLE93_07475"
     CDS             complement(913..1596)
                     /locus_tag="PLE93_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH86926.1"
                     /translation="MTYNEKDSIRHVIEDFEATGVVDEVLVVNNNAARGTAEEVEATG
                     ARQVFETDQGYGHAIRRGLTEATGDLVVLAEPDGTFLATDIIKLLAYSDECEVVLGTR
                     TTNELIWEGANMDWFLKWGNWAVAKMVELIFNTSHLSDIGCTYRLLSRDVARKFAAEM
                     QVGGSHAGPEMLMLTIRDGIRFVEIPVNYLPRVGKSSVTGSRITAFFLGLTMIRLILG
                     FRFGRRSVI"
     gene            1717..>1935
                     /locus_tag="PLE93_07480"
     CDS             1717..>1935
                     /locus_tag="PLE93_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86927.1"
                     /translation="MIAIGSAITEEDIFDAYAGPGIERIREPDTELLIYPSAGSIFRS
                     YNVLLDKAKEIEGLEALVLIHQDDEIVDP"
BASE COUNT          315 a          631 c          670 g          319 t
ORIGIN      
        1 gggggggggg gggggggggg gggggggggg gggggggggg gggggctggt cggccagccg
       61 ctgatcgagc cgggagccgt acccggcaag ctggcgatgt tcctcttcgc ggccgggctt
      121 gcggtcgggc tggccggcgt cgtcctgaaa ctggttcgtg accgggacct gatggtcggg
      181 gggaagtcgg tcctgcccgg gctcagcctg gttgccctga tgaccctttc ggttccggtc
      241 gcgcagctgc tcctgcttct gctcggcacc gatcttttcg gcgcccgcaa cctcgccccc
      301 gcctgggccg ggctgccggt cttgatcggg gccctgatca gcctgcccgc cttctcgctg
      361 gccgcggtag cgacggcatt gacggtcggc gccttcctga tcggggcggt gcggatgacc
      421 gacccggacc tggccagcac cggttacggc ggcgcggccc gtttcatcga ggccaacgcc
      481 gggccggggg atgtggtgat ggactcgagc catttcaccc cggtgccgct gaccgagctc
      541 gacaccgagc tgtcacagga ctggcccgag taccgcgtga acctgccgat cggcgacccg
      601 ccgttcctgc cggtcctggc ccaggtagtg ccgccggacg agcagatcgc cgaggccttc
      661 ggcaaggcag cccgggagga tggaaaggtc ttcgccgtca cctggaaccc gcccctgacc
      721 gatgccagca aggacgggct ttacgcgccc gaaggtgcga cccggggcat tccgaagggc
      781 tggcacctga ccgaaaccag gacctgggat ggctatcgcc cggtcacggt gaacgtcttc
      841 gagcagtcgc cttcgcaata aggagaagga gccggactgg cgcgcaaccg cagggccgaa
      901 cccggcggcc ggtcagatca cggaacggcg accgaagcgg aagcccagga tcaatcggat
      961 catggtcagg ccgaggaaga aggcggtgat gcggctgccg gtcaccgagg acttgccgac
     1021 ccgcggcagg tagttgaccg ggatctcgac gaagcggatc ccgtcgcgga tggtcagcat
     1081 cagcatctcg gggccggcat gggagccgcc gacctgcatc tcggccgcga acttgcgggc
     1141 cacatcccgg ctcagcagcc ggtaggtgca gccgatgtca ctgaggtggc tggtgttgaa
     1201 gatcagctcg accatcttcg ccacggccca gttgccccac ttgaggaacc agtccatgtt
     1261 ggccccttcc cagatcagtt cgttggtggt gcgggtcccg agcacgacct cgcattcatc
     1321 cgaataggcg agcagcttga tgatgtccgt ggcgaggaag gtgccgtccg gttcggccag
     1381 gacgaccagg tcaccggtcg cctcggtgag cccgcgacgg atcgcgtggc cgtacccctg
     1441 atcggtctcg aagacctgcc gggcaccggt cgcctcgact tcctcggccg tgcccctggc
     1501 ggcgttgttg ttcaccacca gcacctcgtc gaccaccccg gtcgcctcga agtcctcgat
     1561 cacatgccgg atcgagtcct tctcgttgta ggtcataagg accacgctga ctgatttccc
     1621 gctccacata agaagcccgg ccggaaggta ccggcggggg cggccagtat aggcttcgcg
     1681 ctcgcccatg actgagccca cgcaggggag aaaccaatga tcgccatcgg cagcgcgatc
     1741 accgaagagg acatcttcga cgcctatgcc ggtcccggca tcgagcgcat tcgcgagccg
     1801 gacaccgaac tgctgatcta cccgtcggcc ggatcgatct tccgcagcta caacgtgttg
     1861 cttgacaagg cgaaggaaat cgagggtctc gaggcgctgg tcctgatcca ccaggacgac
     1921 gaaatcgtcg atccc
//
LOCUS       DAOQFI010000411         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_36715_length_1932_cov_3.971763, whole genome shotgun sequence.
ACCESSION   DAOQFI010000411 DAOQFI010000000
VERSION     DAOQFI010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36715_length_1932_cov_3.971763"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1297
                     /locus_tag="PLE93_07485"
     CDS             149..1297
                     /locus_tag="PLE93_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH86928.1"
                     /translation="MDELLALAEQLIGYDTSSEDGVHEAAGFIKGWLDSRGVETEQGV
                     CRGLPVITASHGDPADPTVILHGHIDVVPAGAGQFEARVEGDRLIGRGTYDMKAALAA
                     MMLTLPTEAEPLPGLRVVLGIVSDEESEEAENRGSNFLVREPDAAGPDRNGGTAATGG
                     MGLSGDFAITGEPTDMHVGVAAKGVLGLRLTVAGRAAHGSTPWLGENAVLLAVDMFRG
                     VESLPFARESSELFDRPSINLGRIWGGDALNKVPDSCAIDVDIRYLPHQDPAQIRDQI
                     AGLGPARMETIMELPPVSGGGTDSPWIQALTAAASRHHEGLSLSVGRDGASDAVSFIR
                     AGIPAVEFGPAGAGHHGPDEWVSIPSLIAYRSALKDFLKRAAKSAGRG"
     gene            1302..>1932
                     /locus_tag="PLE93_07490"
     CDS             1302..>1932
                     /locus_tag="PLE93_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86929.1"
                     /translation="MTEEQSEPEEREEPPAEEPVVEEPPAEEPVAGTPEPDPGEYADP
                     PTEELFAFEARVEEGEVPDLDPVFRAALLESSESALGEQPPSSEPPAGTGESAAAPEA
                     DQQPTTGEEQNTGEQPLTGEEPYTGEEPVTGTSEFEPPTDTGITAEQTAVLQATARSE
                     VHDAIEYSRRTGNAWDPPRPPTGGDFDKPAKRPRYWWRFLLATLLIVFSF"
BASE COUNT          337 a          630 c          662 g          303 t
ORIGIN      
        1 gcccggaggt gccgcatatt cagcaccatc acggggctga atttgcggca ccttatgtcg
       61 attaggaggc acgacacttt ggttgcttct gtgggatggc gggttgacgg gggaggcggg
      121 gtgaagcccg ccgacttatg attcctcgat ggacgaactg ctcgccctgg ccgaacagtt
      181 gatcggttat gacacctctt cggaagacgg ggtgcacgag gctgccggtt tcatcaaggg
      241 ctggctcgac agccggggcg tggagaccga gcagggggtc tgtcgcgggc tgccggtgat
      301 cactgcatcg catggcgatc cggccgaccc gaccgtgatc cttcacggtc acattgatgt
      361 ggttccggcc ggagccgggc agtttgaagc ccgggtcgag ggggatcgcc tgatcggccg
      421 cggcacctat gacatgaagg ccgcgctcgc cgcgatgatg ctgaccctgc cgaccgaggc
      481 cgagccgttg ccggggctcc gggtcgtgct tggcatcgtc tcggacgagg aatcggaaga
      541 agccgagaac cgtggcagca actttctcgt ccgtgagccg gatgccgccg gcccggaccg
      601 aaacggcggg acggcggcca ctggcggcat gggcctgagc ggggacttcg cgatcaccgg
      661 cgaaccgacc gacatgcatg tcggagtggc ggccaaggga gtcctcggtc tccgcctgac
      721 cgtggccggc cgggcggccc atggttcgac gccctggctg ggcgaaaacg ccgttttgct
      781 tgccgtggac atgttccggg gggtcgaatc actacccttt gcgagagaaa gctccgagct
      841 cttcgaccgc ccctcgataa atcttggccg gatttggggc ggcgacgcgc tcaacaaggt
      901 tcccgattct tgcgcgatag acgtagacat ccgctacctg ccacaccagg acccggcaca
      961 gattcgcgat cagatcgcgg ggctcggccc tgcccggatg gagacgatca tggaactgcc
     1021 gccggtcagc ggcggcggta ccgactcgcc ctggatccag gctctgaccg cagcggcctc
     1081 aaggcatcac gagggcctgt ccctttcggt cggccgggat ggagcctccg atgccgtttc
     1141 gttcatccgc gccgggattc cggccgtcga attcggcccg gcgggagctg gtcaccacgg
     1201 ccccgacgaa tgggtttcaa tcccctcgct gatcgcctac cggtcggccc tgaaggactt
     1261 tctgaagcgc gccgcgaaat cagccgggag ggggtgatcc gatgaccgag gagcaaagcg
     1321 aacccgaaga gcgggaagag ccgccagcgg aagaaccggt tgtcgaagag ccgccagccg
     1381 aagagccggt tgccgggact cccgagccgg acccgggcga atatgccgac cctccgaccg
     1441 aggagctctt cgccttcgag gcgcgggtcg aagagggcga ggtgcccgat ctcgatccgg
     1501 tcttccgggc ggccctgctc gaaagctcgg agagcgcgct cggcgaacag ccgccttcat
     1561 ccgaaccgcc ggccggcacc ggggaatcgg cagccgcccc ggaggccgat cagcaaccga
     1621 ccaccggcga ggagcaaaac accggtgagc agccgctgac cggcgaagag ccgtacacgg
     1681 gcgaggaacc ggtgaccggc accagcgagt tcgaaccgcc caccgacacc gggatcaccg
     1741 ccgagcagac cgcggtcctc caggcgacgg ctcgcagcga ggtccatgac gcgatcgaat
     1801 actcccgtcg caccgggaat gcctgggatc cgccccggcc gccgacggga ggtgacttcg
     1861 acaagccggc caagcgcccc cgctactggt ggcgtttcct cctggcgacc ctcctgatcg
     1921 tcttttcctt cg
//
LOCUS       DAOQFI010000412         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_36877_length_1928_cov_3.329418, whole genome shotgun sequence.
ACCESSION   DAOQFI010000412 DAOQFI010000000
VERSION     DAOQFI010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36877_length_1928_cov_3.329418"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLE93_07495"
     CDS             <1..482
                     /locus_tag="PLE93_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNH86930.1"
                     /translation="GPIRVRGTAVYLNARTNTAPLAFRSALERDGIVHDHVVVLSLVT
                     ARQPYVEDKDRIVIDDLGYDYDGISHITATVGFMDVPDVPYLLELANPQVPDGPLDFS
                     KAYYYLSNVLIRPYGDTRLHRLLKHLYITMARNASSPVDHFRLPAERTVIESSGISI"
     gene            complement(465..1637)
                     /gene="chrA"
                     /locus_tag="PLE93_07500"
     CDS             complement(465..1637)
                     /gene="chrA"
                     /locus_tag="PLE93_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNH86931.1"
                     /translation="MPVDQDSRAAIFWRFLKFGLLAWGGPAAQIEMIRHECVEQEKWI
                     SAETFKKQLAVYQILPGPEAHELCVYLGRVRGGKLGGFLAGLGFMLPGFILMLGLSVL
                     YVKASLAGHLDQVFYGLTAAVGALVARAVVRLSKNFLTDIPLVIIAALAFGLTLGISA
                     SFVLVLLGAGIAYELWTNRHGWTRRTNSFGFSPLVLVLAATGAITLSLTAEIFFEGLK
                     AGLLTFGGAFTVIPYLQESAVNNHHWLTDSQFVDGLAMSGVLPAPLIIFSTFVGYIAG
                     GMTGALVMTLGIFLPAFIFPIFFHRQLVAVAENARLHPFLLGVAAGVIGLIAAVTVSI
                     LQTSVVDVWTGLIALGAFLALQKWHSKLTVVYVMAAAGLIGVPLQGNRRLSSDRDP"
     gene            complement(1719..1868)
                     /locus_tag="PLE93_07505"
     CDS             complement(1719..1868)
                     /locus_tag="PLE93_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86932.1"
                     /translation="MAESLSPSPGDDGPDLDALGVSGEDIRNFALGGLTRRLEIIGVP
                     LETVF"
BASE COUNT          398 a          632 c          598 g          300 t
ORIGIN      
        1 cggggccgat ccgggtcaga ggcaccgcgg tctatctgaa cgcccgaacc aacaccgctc
       61 cgctggcctt ccgctcggcc ctcgagcgcg acgggatcgt ccacgaccac gtggtggtgc
      121 tctccctggt caccgcccgc cagccctatg tcgaggacaa ggaccggatc gtgatcgatg
      181 acctcggcta cgactacgac gggatctccc acatcaccgc cacggtcggt ttcatggatg
      241 ttcccgacgt tccctatctg ctggagctcg ccaaccccca ggtccccgac ggcccgctcg
      301 atttcagcaa ggcctactac tacctctcca acgttctgat ccgcccgtac ggtgacacca
      361 ggctccaccg cctcctcaag cacctctaca taacgatggc ccgaaacgcc tcaagcccgg
      421 tagaccactt ccgcctcccc gccgaaagaa ccgtgatcga aagttcaggg atctcgatct
      481 gagctcagac gacggttgcc ttggagcggg actccgatca gcccggcggc ggccattaca
      541 tagacgactg tcagcttcga gtgccacttc tggagggcga ggaaagcccc gagggcgatc
      601 agcccggtcc agacatcgac gacgctggtc tggaggatcg agaccgtgac cgcggcgatc
      661 agcccgataa cccccgcagc gactcccagc aggaacggat gaagccgggc attttcggcg
      721 accgcgacga gctggcggtg aaagaagatc gggaagatga aggcgggcag gaagatgccc
      781 agggtcatga ccagggcgcc ggtcattccg ccggcgatat atccgacgaa ggtggagaaa
      841 atgatcaacg gtgccggcag gacaccactc atcgccagtc cgtcgacaaa ctggctgtca
      901 gtcagccagt gatggttgtt gacggcgctt tcctggaggt agggaatgac cgtgaaggcc
      961 ccgccgaagg tcagcagccc ggccttcagg ccttcgaaga agatctcggc cgtcagggaa
     1021 agggtgatcg ccccggtcgc cgccagcacg agcaccaggg gactgaaccc gaacgaattg
     1081 gtccgccggg tccagccatg ccggttggtc cacagctcgt acgcgatgcc ggcgccgagc
     1141 aggacgagca cgaagcttgc gctaatgccc agggtcagtc cgaaggcgag cgcagcgatg
     1201 atgaccaacg gaatgtcggt gaggaagttc ttcgagagcc ggaccacggc gcgggccacc
     1261 agggcgccga ccgctgccgt cagtccgtag aagacctggt ccagatgccc ggccaggctg
     1321 gccttgacgt agaggaccga gagccccaac atgaggatga agccgggcag catgaagccg
     1381 agaccggcga ggaatccgcc gagcttcccg ccccgcaccc ggcccaggta gacacagagc
     1441 tcatgggctt ccggtccggg aagtatctgg tagacagcga gctgcttctt gaaggtctcc
     1501 gcggagatcc acttctcctg ctcgacgcac tcgtgcctga tcatttcgat ctgggccgcc
     1561 ggtcctcccc aggcgagcaa accgaacttg aggaagcgcc agaagatggc tgctcgggaa
     1621 tcctggtcaa cgggcatggc agcttttacc aagttccggc cgcatccggg ttacttcccg
     1681 gagagaaagc gcgcagacgc cggtgaccac ccgcagttct agaagaccgt ctcgagtgga
     1741 acgccgatta tctccaggcg cctggtcagg ccgcccaggg cgaagttccg gatgtcctcg
     1801 ccactgacac cgagagcgtc gaggtccggg ccatcatctc cgggagacgg cgagagcgat
     1861 tcggccacct aaggcagcag cgtccgaagc atcgaccgga tggcctcagg cgccacatcc
     1921 ggtgactc
//
LOCUS       DAOQFI010000413         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_36911_length_1927_cov_2.682158, whole genome shotgun sequence.
ACCESSION   DAOQFI010000413 DAOQFI010000000
VERSION     DAOQFI010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36911_length_1927_cov_2.682158"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..448)
                     /gene="crcB"
                     /locus_tag="PLE93_07510"
     CDS             complement(47..448)
                     /gene="crcB"
                     /locus_tag="PLE93_07510"
                     /inference="COORDINATES: protein motif:HMM:NF010812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNH86933.1"
                     /translation="MFQRHPLRKLTAIYVGGVVGALLRVGLAEAFPHAVDAWPWPTFA
                     VNLAGAFLIGYFFTALAGQAPDRLHHPFLATGICGTLTTFSTLQLEIFNMVDGGEPGL
                     AFAYAALTVALGYLAVRLGIVAARPGPGVRP"
     gene            complement(473..910)
                     /locus_tag="PLE93_07515"
     CDS             complement(473..910)
                     /locus_tag="PLE93_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNH86934.1"
                     /translation="MKASWKYLVVPLAVGALIFAGCGSDDSSDDSGSSDTTTTQSQPA
                     DNSAMGGQVLKLAADPSGALAYDTTELSAKSGKVTLDFTNDSPVGHDVVIEDSNGKEV
                     GSTDVITGQSTTAEFTVKPGTYTFYCSLPGHEEAGMKGTLTVK"
     gene            1013..>1927
                     /gene="cofG"
                     /locus_tag="PLE93_07520"
     CDS             1013..>1927
                     /gene="cofG"
                     /locus_tag="PLE93_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HNH86935.1"
                     /translation="MRRVTFSRNYTISLSRTCQCYCKYCAFATHQAHVHPPGEVERML
                     DEAVKRRAKELLFLTGEKPEVNPAVKEQLRSWGHDDFTSYVIWACERAMERGILPHTN
                     IGAIGKEDLARLREVTASQGLMLESSSERLMKTVHAGSPTKHPAHRLKTIEAAGQLKI
                     PFTTGILVGIGETPEERIESLEALAALQQKYGHIQEVIIQNFVPHPRYYGQEPADIAA
                     EASDRRWKESGTVVSLDSGIEAGAGEETANHDPGRPTIPLPDWACPVSIGDLKHLIRE
                     TARLMPGVGVQVPPNLSDWWAELVEAGAT"
BASE COUNT          369 a          610 c          636 g          312 t
ORIGIN      
        1 aacccggcga ccgcagccca gccgaacagg ctcacaccaa agacggtcaa ggccgaaccc
       61 ccggccctgg tctggcggcc acgatgccca gcctcactgc gaggtagccg agggcaacgg
      121 tgagggccgc gtaggcgaag gccagcccgg gctccccgcc gtcgaccatg ttgaagattt
      181 cgagctggag ggtcgagaag gtggtcaggg ttccgcagat cccggttgcc aggaaagggt
      241 ggtgcagccg gtcgggcgcc tgaccggcga gcgcggtgaa gaagtaaccg atcaggaatg
      301 ccccggccag gttgaccgca aaggtgggcc agggccaggc atcgaccgcg tggggaaagg
      361 cttcggcgag gccgaccctc aagagggccc cgacgacgcc acccacatag atcgcagtga
      421 gctttctaag tgggtggcgc tgaaacatca gggcaggaag gtaccccttc gtctacttga
      481 cggtgagcgt tcccttcatt cccgcttcct cgtggcccgg cagcgagcag tagaaggtgt
      541 aggtgccagg cttgaccgtg aactcggccg tcgtgctctg cccggtgatc acatcggtcg
      601 aaccgacttc cttgccgttc gagtcctcga tcaccacgtc gtggccgacc ggcgaatcat
      661 tcgtgaagtc gagcgtgacc ttgcctgact tggcggacag ctcggtcgtg tcataagcga
      721 gggcacccga cggatcggca gccagcttca gaacctgtcc gcccatggca ctgttgtcgg
      781 cgggctggct ctgggttgtg gtggtgtcgc tggatccgct gtcgtcggat gaatcgtcgc
      841 taccgcagcc ggcgaagatc agggctccaa ccgcaagcgg tacgaccaga tacttccatg
      901 aagctttcat cgcgttctcc ttggggggtc gatggacggg aagtgcattc acactacccg
      961 ggtgctacgc cgggccgaag cggaaggttc actgcggtag gttggaagcc ggatgcgtcg
     1021 ggtcaccttt tcgcgcaatt acacgatctc gctttcgcgg acctgccagt gctactgcaa
     1081 gtactgcgcc ttcgctacgc atcaggccca tgttcacccg cccggcgaag tcgagcgaat
     1141 gctggacgag gcggtcaagc ggcgggccaa ggaactgctc ttcctgaccg gcgagaagcc
     1201 ggaagtcaac ccggccgtga aggagcagct gcgctcctgg gggcacgatg acttcacctc
     1261 atatgtgatc tgggcctgcg aacgggcgat ggagcggggg atccttcccc acaccaacat
     1321 cggcgcaatc ggcaaggagg acctggcacg actgcgcgag gtcaccgcct cccagggcct
     1381 gatgctcgaa tcgtcttccg agcgcctgat gaagaccgtt cacgccggct cgccgaccaa
     1441 acatccggct catcgcctga agacgatcga agcggccggc cagctgaaga ttcccttcac
     1501 caccggcatc ctggtcggaa tcggcgagac cccggaagaa cggatcgagt cgcttgaggc
     1561 gctggccgcc ctgcagcaga agtacggcca catccaggaa gtgatcatcc agaacttcgt
     1621 gccgcatccc cgctactacg ggcaggaacc ggctgacatc gcggccgaag cttccgaccg
     1681 gcgctggaag gaatcgggga cggtcgtgag cctcgattcc ggcatcgaag ccggggccgg
     1741 cgaagaaacg gcaaaccacg accccggacg cccgaccatc ccgctgccgg actgggcctg
     1801 cccggtatcg atcggggacc tgaagcacct gatccgcgaa acggcccggc tgatgccggg
     1861 ggtcggggtc caggtgccac cgaacctttc cgactggtgg gcggagctgg tcgaggccgg
     1921 ggcaacc
//
LOCUS       DAOQFI010000414         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_36938_length_1926_cov_3.004276, whole genome shotgun sequence.
ACCESSION   DAOQFI010000414 DAOQFI010000000
VERSION     DAOQFI010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36938_length_1926_cov_3.004276"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="glyS"
                     /locus_tag="PLE93_07525"
     CDS             complement(<1..635)
                     /gene="glyS"
                     /locus_tag="PLE93_07525"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNH86936.1"
                     /translation="MSDYATDKNTEVTMEEIVALAKRRGFVFPSSEIYGGLGSTYDYG
                     HYGVLLKNNVKNEWWKSMLVERDDIVALDSAILQHPKVWEASGHLAGFTDPLVQCLGE
                     CKRRWREDHLREANVEKGGNYDDPIVCPECGGELSPPRNFNLMFETHMGPVADDGNAV
                     YLRPETAQGIFINFKNVLNFARKKPPFGIAQVGKSFRNEITPGNFIFRTRE"
     gene            723..1088
                     /locus_tag="PLE93_07530"
     CDS             723..1088
                     /locus_tag="PLE93_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH86937.1"
                     /translation="MPIYEYRCENGHLFEVMQSMSEDALTECIECGAPVQKVLHSPAI
                     HFKGSGFHNTDYGTRKSNKAKAAAESAKKDSGSSSSSSGSSDSGSSSSGSSDSGSGSS
                     SSSSSDSGSKASSGSSNSD"
     gene            1117..1416
                     /locus_tag="PLE93_07535"
     CDS             1117..1416
                     /locus_tag="PLE93_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNH86938.1"
                     /translation="MYTRYMDRIGIRELRADLSNQVKRAAAGEAVVVTIDGEPKALLT
                     PFVAESGEMTLEELIRTGRVIPGPRVNEPRPPRRQGIKGGRPTKDVLDEIREDKI"
     gene            1416..1823
                     /locus_tag="PLE93_07540"
     CDS             1416..1823
                     /locus_tag="PLE93_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH86939.1"
                     /translation="MLCFDSSALVKCYQEEEHSPWVREIMASDPDWCGSALLAAEGPI
                     ALSRSVPEADDLQVADARISRDLGFFFLVAVDADCLVRAVDIGRDFGLRTLDAIHLAA
                     ARILPSECSFVTFDDRQREAAESLGLKVLTPPV"
BASE COUNT          358 a          559 c          620 g          389 t
ORIGIN      
        1 aactcgcggg tccggaagat gaagttgccc ggcgtgatct cgttgcggaa cgacttgccg
       61 acctgggcga tcccgaaggg cggcttcttg cgggcgaagt tgagcacgtt cttgaagttg
      121 atgaagatgc cctgggctgt ctcgggccgc aggtagacgg cgttgccgtc atcggcgacc
      181 ggacccatgt gggtctcgaa catcaggttg aagttgcggg gaggggagag ctcgccaccg
      241 cattccgggc agacgatcgg gtcgtcgtag ttaccgccct tttccacgtt ggcctcgcgc
      301 aggtggtctt cacgccagcg gcgcttgcac tcgccgaggc actgcaccag cgggtcggtg
      361 aagccggcca ggtgcccgga ggcctcccag accttggggt gctgcaggat cgccgagtcg
      421 agggcgacga tgtcatcgcg ctcgaccagc atcgacttcc accattcgtt cttgacgttg
      481 ttcttgagca gaactccgta atggccgtag tcgtaggtcg aaccgaggcc gccgtagatc
      541 tccgaagaag ggaagacgaa accgcgccgc ttggccagcg caacgatctc ttccatggtc
      601 acttcggtgt tcttgtcggt ggcgtagtcg ctcatgtcct agaggttatg gacaatcgtc
      661 ccgccaagcc gggacgggta tcggggcgga aacgggggcg cgctgcgata atcagccgcg
      721 ctatgccgat ttacgaatac cgctgtgaaa acgggcacct cttcgaggtc atgcagtcga
      781 tgagcgagga cgccctgacc gaatgcatcg aatgcggcgc ccccgtccag aaggtgctgc
      841 attcaccggc gatccacttc aagggttccg gtttccacaa caccgactac ggcacccgca
      901 agtccaacaa ggcgaaggcc gcagccgaat cagcgaagaa ggacagcggc tcgagctcca
      961 gctcgtcggg ctcctccgac tcgggatctt cgagttccgg ctcatccgat tccggttccg
     1021 ggtctagctc gtcgagctct tcggactccg gttcaaaagc ctcgtcgggt tcttcaaact
     1081 ccgactgagc aaggtcggtt tttcgggtgt accgtcgtgt acactcgcta tatggatcgg
     1141 attggtatca gggaactccg cgcggacttg agcaatcagg tgaagcgtgc tgccgccggt
     1201 gaagctgtgg tcgtcacgat cgatggagag ccgaaagccc ttctgacacc gttcgtcgcg
     1261 gagtctggtg agatgactct ggaagagctg atcaggaccg gtcgggtaat tcccggaccg
     1321 agagtcaacg agcccagacc acctcgacgg caagggatca aaggagggcg tccgacgaag
     1381 gatgttctgg acgagatccg cgaggacaag atctgatgtt gtgcttcgat tcgtcggctc
     1441 tggtcaagtg ctaccaggag gaagagcatt caccgtgggt tcgcgaaatc atggcgagcg
     1501 accctgattg gtgtggttcc gctctgctgg cggcggaagg accgatcgcg ctgtctcgct
     1561 ccgtacccga agcagacgat ttgcaggtcg ctgacgccag gatcagcagg gatctgggct
     1621 tcttttttct cgtggcagtc gacgcggact gcctcgttcg ggcagtcgac atcggtcggg
     1681 atttcggctt gagaaccctg gacgcaatcc acctggcggc ggccagaatc ctcccgtccg
     1741 aatgcagttt cgtcaccttc gacgaccgcc agcgcgaagc ggccgaatcc ctcggcctga
     1801 aggtccttac tccgccggtc tagccagcgc ggctaccgcg cttcagggat ctggcaggtt
     1861 cagcgatttc ggacggtatg gcggggtctg gcgtcgaatg cccactttgt gccggtatgt
     1921 ggcaca
//
LOCUS       DAOQFI010000415         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_37086_length_1922_cov_4.289770, whole genome shotgun sequence.
ACCESSION   DAOQFI010000415 DAOQFI010000000
VERSION     DAOQFI010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37086_length_1922_cov_4.289770"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..657
                     /locus_tag="PLE93_07545"
     CDS             94..657
                     /locus_tag="PLE93_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNH86940.1"
                     /translation="MSSKIKSRAAGLLSVGIVALALGAASAVPATASASGDGAASSSS
                     LTAKQKKAKRAELKKCYRIKAKAKRKACIKRVNKKYAKLAKPPVKVAATIDVLDDYYY
                     PSDVTINRGEAVKWVWSNSNANGHTVSLDDPFPVGLTASEKYSLNTPTAPATFFTFGP
                     KQLKKPGVYHFVCELHSATMKMTVTVK"
     gene            704..1729
                     /locus_tag="PLE93_07550"
     CDS             704..1729
                     /locus_tag="PLE93_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNH86941.1"
                     /translation="MDRRNFLGLAGGAFLCTIAGKQYSIDSNTDLAALANKIEVPPKV
                     AAAKRSNSVSNADISGNRKEYWIQAEPVTWNVVPKQRDQMMGTSIKKTVGRTKVPAYA
                     YRLYDTDFSKPLGPATVPGPLLDVTVGDTLVVNFRNACDVPVTMHPHGVFYSNEMDGA
                     YKGYWTDPGGFVQKGRTFQYVWECPEGTQGSWLYHDHGPFDSTAVFKGLFGPLIVRDP
                     DEPLPDREYFLGFHSYLPGLFPLKAPLFAINGRAYAGNTPTMRATVGDDVAMHVYGVD
                     NDMHTFHVHGHRWQEAGGTYADNKIFGPGDSFRVRWIEQNPGRWLYHCHVFSHLVTGM
                     SGWYIVD"
     gene            complement(1732..>1922)
                     /locus_tag="PLE93_07555"
     CDS             complement(1732..>1922)
                     /locus_tag="PLE93_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86942.1"
                     /translation="PNLIRAKNDYLIAEINLGVLVRPVEIGDRDPGVVRGRRGGKSGS
                     DDRGRKYGDERPERPAGA"
BASE COUNT          394 a          626 c          576 g          326 t
ORIGIN      
        1 gtacaccgag gatcgggccg acccggtgac ctgctcctga ccaggagcag ttctgccccc
       61 agccaaacca caattcaaga ggtaagaatc aaaatgagca gcaagatcaa gtcaagagca
      121 gcagggttgc tgtccgtggg aatcgtcgcg ctggcgctgg gggccgccag cgcggttccc
      181 gccactgctt cagcgtcggg tgacggagcc gcgtccagct ccagcctgac cgcgaagcag
      241 aagaaggcca agagggccga actgaagaag tgctacagga tcaaggcgaa ggcgaagcgc
      301 aaggcctgca tcaagcgggt caacaagaag tacgcgaagc tcgccaagcc tccggtcaag
      361 gttgccgcca cgatcgacgt cctcgacgat tactactacc cgtccgatgt gaccatcaac
      421 cggggcgagg ccgtcaagtg ggtgtggagc aactcgaacg cgaacggtca caccgtttcc
      481 ctggatgatc cgttcccggt cgggctgacc gccagcgaga agtactcgct gaacactcca
      541 accgcgccgg caaccttctt caccttcggc cccaagcagc tgaagaagcc gggcgtctat
      601 cacttcgtct gcgagctcca ctcggcgacg atgaagatga cggtcacggt caagtaaccg
      661 ggcgagcatc aggtctggca agagccagaa aggactggat ccaatggacc gcaggaattt
      721 cctcggactg gccggtggtg cgtttctctg caccatcgcg ggcaagcagt actcgattga
      781 cagcaacacc gacctcgccg ctttggcgaa caagatcgag gtaccgccga aggttgcggc
      841 tgcaaaacgg agcaattccg taagcaacgc cgacatctcc ggcaaccgca aggagtactg
      901 gatccaggcc gaacccgtca cctggaacgt ggtccccaag caacgggacc agatgatggg
      961 tacgtcgatc aagaagacgg tcgggcggac caaggtcccg gcctatgcgt atcgtcttta
     1021 cgacaccgac ttcagcaagc cgctgggtcc ggccaccgtg ccgggcccgc tgcttgacgt
     1081 cacggtcggt gacaccctgg tcgtcaactt ccgcaacgcc tgcgacgtcc cggtcaccat
     1141 gcacccgcac ggcgtcttct actcgaacga gatggacggc gcctacaagg gctactggac
     1201 cgacccgggc ggattcgtcc agaagggccg caccttccag tacgtctggg aatgccccga
     1261 aggcacccag ggcagttggc tctaccacga ccacggacct ttcgattcga ccgcggtctt
     1321 caagggcctt ttcgggccgc tgatcgtccg cgacccggac gagcccctgc cggaccgcga
     1381 gtacttcctc ggttttcatt cgtacctgcc cggcctgttc ccgctgaagg ccccgctttt
     1441 cgcgatcaac ggccgtgctt acgccggcaa cacgccgacc atgcgggcca cggtcggtga
     1501 cgacgtcgcg atgcatgttt acggcgtcga caatgacatg cacaccttcc acgtccacgg
     1561 tcaccgctgg caggaagcgg gtggcaccta cgcggacaac aagatcttcg gcccgggtga
     1621 ctccttccgg gtccggtgga tcgagcaaaa ccccgggcgc tggctctacc actgccatgt
     1681 gttcagccat ctcgtgacgg gaatgagcgg ttggtacatc gtcgactgac gtcaggcgcc
     1741 ggccgggcgc tccgggcgct catcgccgta tttgcggccg cggtcgtcgc ttccgctttt
     1801 accgccccgg cgtccgcgaa caacacccgg gtcacgatcg ccgatttcca ctggtcgaac
     1861 aagaaccccg aggttgatct cggcgatcag gtaatcgttc ttcgcgcgga tcaggttcgg
     1921 ga
//
LOCUS       DAOQFI010000416         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_37289_length_1917_cov_3.250806, whole genome shotgun sequence.
ACCESSION   DAOQFI010000416 DAOQFI010000000
VERSION     DAOQFI010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37289_length_1917_cov_3.250806"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLE93_07560"
     CDS             <1..346
                     /locus_tag="PLE93_07560"
                     /inference="COORDINATES: protein motif:HMM:NF021883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNH86943.1"
                     /translation="LPDATIGLDYMHHVITQVASGLVVHVDRLQANLEQTNGAMFSQR
                     ALTALVESGMTRDDAYRTVQAAAHEAFDGDTHFRELIGKAAPELDLDEIFDYNAYLTH
                     LPEVFERLEEIR"
     gene            408..698
                     /locus_tag="PLE93_07565"
     CDS             408..698
                     /locus_tag="PLE93_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase domain-containing
                     protein"
                     /protein_id="HNH86944.1"
                     /translation="MKLYTCGTGTSGPAGVHPCAKAGKALDDAGYEYELRKVRGYRML
                     PWTWKSRAEDRKEVKELSGTNEVPILVLDDGEVISDSSAIAKWAKENPAPGR"
     gene            799..1323
                     /locus_tag="PLE93_07570"
     CDS             799..1323
                     /locus_tag="PLE93_07570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH86945.1"
                     /translation="MWLLPDDRVRARVERRQYRSVVRHIFVSRRSGWISLREDGEAAG
                     AVLWYPPGTKKHSFRESLAESIPFLPAGLPRLGKATRLQKLVVSNWPKEPHWYLSVLS
                     VSPESQRQGHGGALLGPGLARADRQGVGCWLETQRESNIPFYARFGFELVKKLEIEAG
                     VNLWLMWRPPLEGD"
     gene            complement(1365..>1917)
                     /locus_tag="PLE93_07575"
     CDS             complement(1365..>1917)
                     /locus_tag="PLE93_07575"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNH86946.1"
                     /translation="AAKKKSLVSDTLGMANGVARGKDGYIYASNNLGLKLDRITPDGD
                     TENGWASLNSANGLTVGRNGRYLYANQMFETPSTIAKIDTRDPTKVFTYFTSPEAGNV
                     LLDGLAHDSGNNLYTAAYGKGEVWKITPDRQACVIATGLSQTTAVAMSSAGKGFRAGS
                     LYAAGFDGNIVEVTGAAKAAVPG"
BASE COUNT          322 a          623 c          649 g          323 t
ORIGIN      
        1 cctgcccgac gcaacgatcg gcctcgacta catgcatcac gtgatcaccc aggtcgcttc
       61 cgggctggtc gtccatgtcg accggcttca ggcgaacctc gagcagacca acggcgcgat
      121 gttcagccag cgggccctga ccgccctggt cgaatcgggc atgacccgcg acgatgccta
      181 ccgcacggtc caggccgccg cccacgaagc cttcgacggg gacacccatt tccgcgagct
      241 gatcggcaag gccgccccgg agctggacct cgacgagatc ttcgactaca acgcctacct
      301 cacccacctc cccgaagtct tcgaaaggct cgaagagatc cgctaggcag cagggcccgg
      361 cacgggggta tgcaccacgc ctcgccttct gggtttagcc tccaggcatg aagctctaca
      421 cctgcggtac aggcaccagt ggtccggcgg gggtgcatcc ctgcgcaaag gcgggcaagg
      481 cgctcgacga cgccggatac gagtacgaac tcaggaaggt tcgcggctac cggatgttgc
      541 cctggacctg gaagagccgg gccgaggacc gcaaggaggt aaaggagctc tccggcacca
      601 acgaagtgcc gatcctggtg ctcgacgacg gcgaagtgat cagcgactcg tcggcgatcg
      661 ccaagtgggc gaaggagaac cctgcccccg gcaggtaagc ggccaggggc tggccacggt
      721 gtagacctga tcggcccgtc cgaggccagg gccgcggccg atgcgatcgc tcgcgggttt
      781 caggacaacg aagtctggat gtggctgctg cccgatgacc gggtccgggc ccgggtcgaa
      841 cggcggcagt accggtcggt ggtcaggcac atcttcgttt cgcgccggtc gggctggatt
      901 tcccttcgcg aagacgggga agcggcggga gcggtgctct ggtacccgcc gggaaccaag
      961 aagcacagct tcagggagag cctggccgag agcattccct ttctgcccgc cggcctgccc
     1021 cggctcggca aggcaacccg gctgcagaag ctggtcgttt ccaactggcc aaaggaaccc
     1081 cattggtacc tctcggtgct ttccgtctca cccgagtccc agcggcaagg ccatggcggg
     1141 gcgctgctcg ggcccgggct cgccagggcg gaccgccagg gtgtcggctg ctggctggaa
     1201 acccagcgcg aaagcaacat tcccttttac gcccgcttcg gcttcgagct ggtcaagaag
     1261 ctggaaatcg aggccggcgt gaacctctgg ctgatgtggc ggcctcccct cgagggggac
     1321 tgaccgcgag ggcgctgccg ccgggaagcc cgggtggcgg cggatcagcc gggcacagcc
     1381 gccttcgccg cgccggtgac ctcgacgata ttgccgtcga acccggcggc gtagagactg
     1441 ccggccctga agcccttgcc ggcgctactc atcgctaccg cggtggtctg cgaaagtccg
     1501 gtggcgatca cgcatgcctg cctgtccgga gtgatcttcc agacctcgcc cttgccgtag
     1561 gccgccgtgt agaggttgtt gccggagtca tgcgccagcc cgtcgagaag gacgttgcct
     1621 gcctccggcg aggtgaagta agtgaagact ttcgtggggt cgcgggtatc gatcttcgcg
     1681 atggtcgagg gtgtctcgaa catctggttc gcgtagaggt agcgaccatt cctgcctacc
     1741 gtgaggccgt tggcggagtt caggctggcc cagccgttct cggtgtcgcc gtcgggcgtg
     1801 atccggtcga gcttgagacc gaggttgttc gaggcgtaga tgtagccgtc cttgccgcgg
     1861 gcaactccgt tcgccatccc gagagtgtcc gagacgaggc tcttcttctt cgcggcg
//
LOCUS       DAOQFI010000417         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_37338_length_1916_cov_2.369694, whole genome shotgun sequence.
ACCESSION   DAOQFI010000417 DAOQFI010000000
VERSION     DAOQFI010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37338_length_1916_cov_2.369694"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLE93_07580"
     CDS             <1..204
                     /locus_tag="PLE93_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86947.1"
                     /translation="LMRLKERTQQEKMLGKILKQSLSANDFEKEIRASGAKLKHSRQG
                     GRKPKTPSSASPACRRRSGWCSS"
     gene            192..944
                     /locus_tag="PLE93_07585"
     CDS             192..944
                     /locus_tag="PLE93_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNH86948.1"
                     /translation="MQQLNRYEEVATEKVFDVIDEMAPDEVTPALKERLEKTIEQLDT
                     APPDDVFQLVTRPEEAPDEPELVTIGFEAGRPVSFNGERLDLVPLLEAAAEAGCRHGC
                     GIVDHIEDRIVGLKVRDIYEVPAAALVLTAHQDLEKVVSTIHQNNFKHSLDRHWAYMV
                     YAGLWYEPLRHDLDSYMDSANEFITGEVTLKLYKGSVTPVARKSDYAVYDENLASFGA
                     SGGEFSQNAAPGFIELFSLQSRMAHQVRNRKE"
     gene            1023..1202
                     /locus_tag="PLE93_07590"
     CDS             1023..1202
                     /locus_tag="PLE93_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86949.1"
                     /translation="MIYKLIGMAVVKLGWMFLRRKAAANRGLLTGVAAAMVIALLAVV
                     AGTAGYALTRETPEA"
     gene            1410..>1916
                     /locus_tag="PLE93_07595"
     CDS             1410..>1916
                     /locus_tag="PLE93_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86950.1"
                     /translation="MAPTRTVFTALTGGLALALACALLLVSLNSGAASAATLEQKQAA
                     ARDRIQQTSDQIAAGRADLDAAQARADAAAAREADLSGLLASGEARSAELADRLAAAE
                     ADLARSKKRLALARKYLARHLVAVYMHGGEPDTLDMALGAGSFDQLTTGTEYLTAIED
                     TNERLMDRV"
BASE COUNT          351 a          646 c          600 g          319 t
ORIGIN      
        1 ctgatgcgcc tcaaggagcg cacccagcag gagaagatgc tgggcaagat cctcaagcag
       61 tcgctgtcgg ccaacgactt cgagaaggag atccgggcca gcggggccaa gctgaagcac
      121 agccgccagg gcggccggaa gccgaagacc cccagctcgg catcgccggc ttgcagaaga
      181 cgttcgggct ggtgcagcag ctgaaccgct acgaggaggt ggccaccgag aaggtcttcg
      241 acgtcatcga cgagatggcc cccgacgagg tgacgccggc cctgaaggag cggctggaga
      301 agaccatcga gcagctcgac accgctccgc cggatgacgt cttccagctg gtcacccggc
      361 cggaagaggc tcccgacgag ccggaactgg tgacaatcgg attcgaagcg ggtcgtccgg
      421 tcagcttcaa cggcgagcgg ctcgacctcg tgccgctgct cgaggcggca gccgaggccg
      481 gttgccgtca cggttgtgga atcgtcgatc acatcgagga ccgcatcgtc ggcctcaagg
      541 tgcgcgacat ctacgaggtc ccggcggcgg cgctcgttct gaccgcccac caggatctcg
      601 agaaggtcgt ttcgaccatt caccagaaca acttcaagca ttcgctcgac cggcactggg
      661 cctacatggt ctacgccggg ctctggtacg aaccgctgcg ccacgatctc gattcctaca
      721 tggattcagc caacgaattc atcaccggcg aagtcaccct caagctctac aaggggtcgg
      781 tgactccggt cgcccgcaag tccgattacg cggtctacga cgagaacctc gcttccttcg
      841 gggcatccgg cggagagttc tcgcagaacg ccgccccggg cttcatcgaa ctcttttcgc
      901 ttcaaagccg catggctcac caggtccgga accggaagga gtagaggttc ccgccgggca
      961 agccggaggc ccgtgttccg gcagacggtt ctagggtaaa ttcaggacat ggaggcaatc
     1021 aggtgatcta caagctgatc ggaatggccg tcgtcaaact cggctggatg ttcctccgcc
     1081 gcaaggccgc cgccaaccgc ggcctgctga ccggcgtcgc ggcagccatg gttatcgccc
     1141 ttctggccgt ggtcgccggt accgccggtt acgcgctcac ccgcgaaacc cccgaagcct
     1201 gagaaccagc agtcggcgag tttcgcccct ggtatcccct cgaccgcgag gcgtcatttt
     1261 ttcgtcgcca tgacgaaaaa gtcacgcctc atcgacctga cggcctagga agtcccagcg
     1321 ctctgtcctg ctgacgcaag agatcctgca tccgtgagct ggatcacggc tgtttgcggg
     1381 ggtcaccgtt acggtgttcc cttcgcattt tggctccaac tcgtacagtt ttcaccgccc
     1441 tgacgggtgg tcttgcgctg gcgctcgcct gcgcgctcct gctcgtatcc ctgaattcag
     1501 gggcggcttc ggctgccact ctcgagcaga agcaggcggc tgcccgcgac cggatccagc
     1561 aaacttcgga tcagattgcg gccggtcgcg ccgacctcga tgccgcccag gcccgggccg
     1621 atgcggctgc cgcccgcgaa gccgaccttt ccggcctgct cgccagcggc gaggcccgat
     1681 cggcggaact ggctgaccgg ctggccgcag ccgaagcgga cctggcccgc tcgaagaaac
     1741 ggctggccct ggcccgcaag tacctggccc ggcacctggt cgccgtctac atgcacggcg
     1801 gcgagccgga cacgctcgac atggcccttg gcgccggctc attcgaccag ttgaccaccg
     1861 gcaccgagta cctgaccgcg atcgaggaca cgaacgagcg cttgatggac cgggtc
//
LOCUS       DAOQFI010000418         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_37377_length_1915_cov_2.617204, whole genome shotgun sequence.
ACCESSION   DAOQFI010000418 DAOQFI010000000
VERSION     DAOQFI010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37377_length_1915_cov_2.617204"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLE93_07600"
     CDS             complement(<1..272)
                     /locus_tag="PLE93_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86951.1"
                     /translation="MEASCETKGRRGWYLERVLFAMAGTVSLAGGLLALLVSPWFALL
                     PVLAGINQWLYVTVGACPASLVLSRVTSLRSAAETGRASRVVHRELP"
     gene            complement(377..712)
                     /locus_tag="PLE93_07605"
     CDS             complement(377..712)
                     /locus_tag="PLE93_07605"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH86952.1"
                     /translation="MALPTPLPPELVELITARFKLLSEPMRIRILDQLREGEQSVGGL
                     AEILDTSQQNVSKHLQALYAGGAVARRKEGNTVYYGIADESLMSMCEAVCEGVEQRIA
                     ELSQIVTQA"
     gene            complement(766..1479)
                     /locus_tag="PLE93_07610"
     CDS             complement(766..1479)
                     /locus_tag="PLE93_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNH86953.1"
                     /translation="MSTTPQLPTTLDQEIIRCFEPEIQAEMTDPERLAKIQQEFEEGF
                     EVMKPVGPAVCVFGSARTRPGHPEYEQARRVGAGIARQGYAVITGGGPGSMEAANRGA
                     KDEEGLSVGLNIELPHEQGANPFLDIGKEFHYFFVRKLMFVRYSWSFVIFPGGFGTLD
                     ELFEALTLIQTLKARPHPVVLAGADFWQDLIDWIARRLAGEGRISPSDMDLFTVCDED
                     DEIISSAIHGLPNLPYGLA"
     gene            complement(1490..>1915)
                     /locus_tag="PLE93_07615"
     CDS             complement(1490..>1915)
                     /locus_tag="PLE93_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HNH86954.1"
                     /translation="VVASFLTKDLHLDWREGERHFMELLIDGDVASNNGGWQWVASTG
                     TDPAPYFQRMFNPMTQQEKFDPEGEYVRRWVPELRKVPQGHLVRPWKMTGEEQEEAGC
                     LIGKDYPEPVVDHAEERRFAIERYRAAADADPSDGDWRG"
BASE COUNT          337 a          616 c          614 g          348 t
ORIGIN      
        1 ggaagttccc ggtggaccac tcgggaggct cggccggttt ccgcggcgga ccgcagcgaa
       61 gtgacgcggc tgaggaccag tgacgccgga caggcgccga ccgtgacgta aagccactgg
      121 ttgatcccgg ccaggaccgg caggagggcg aaccaggggg agaccagcag ggccaggagg
      181 cccccggcca ggcttaccgt tccggccatc gcgaagagca cccgttcgag gtaccagcca
      241 cggcggccct tggtttcaca gctcgcttcc atcatcgtct ccgatctaaa atacaagttc
      301 acaactgtat agtggaatag attagcagag attcggggcg ccggcttgag cggcgtgaac
      361 gggaggttcc ggaagatcag gcctgcgtga cgatctggct gagctcggcg atgcgctgtt
      421 cgacgccctc gcagacggct tcgcacatcg acatcaggct ttcgtcggca atcccgtaat
      481 agacggtgtt gccttccttg cggcgcgcca ccgcgcctcc ggcgtagagc gcctggaggt
      541 gcttggagac attctgctgg ctggtgtcga ggatctcggc caggccaccg acgctctgct
      601 ctccttcgcg aagctgatcg aggatccgga tccgcatcgg ttccgagagc agcttgaagc
      661 gggcggtgat cagttcaacc agctcgggcg ggaggggagt cggcagggcc atgccttcag
      721 cttacagccg gacaactgtt tgctcgccga gttgcccggc ggcgttcagg cgagtccgta
      781 aggcaggttc ggaaggccgt gaatcgcgct gctgatgatc tcgtcatctt cgtcgcagac
      841 cgtgaagagg tccatgtcgg aaggggagat ccggccctcg cccgccaggc gcctggcaat
      901 ccagtcgatc aggtcctgcc agaagtcggc cccggcgaga acgacgggat gcggccgcgc
      961 cttcaatgtc tggatcaggg tcagcgcctc gaacagctcg tccagcgtgc cgaagccgcc
     1021 gggaaagatc acgaacgacc acgagtagcg cacgaacatc agcttgcgga cgaagaagta
     1081 gtggaactcc ttgccgatgt cgagaaaggg gttcgctccc tgctcatggg gcagttcgat
     1141 gttcaacccg accgagagtc cttcctcgtc cttggcgccg cggttcgccg cctccatcga
     1201 acccggaccg ccgccggtga tcaccgcata gccctggcgg gcgatgccgg ccccgacccg
     1261 acgggcctgt tcgtactcgg ggtggccggg cctggtcctc gccgacccga acacgcagac
     1321 cgccggcccg accggcttca tcacctcgaa tccctcctcg aattcctgct ggatcttcgc
     1381 gaggcgttcg ggatccgtca tctcggcctg aatctcgggc tcgaaacagc ggatgatctc
     1441 ctggtcgaga gtggtgggca gctgtggagt cgtactcatc ggcggcagcc tatccccgcc
     1501 agtccccgtc cgacgggtct gcgtcggccg cggcacggta ccgctcgatc gcgaatcgcc
     1561 gctcctccgc gtgatcgact accggctccg gatagtcctt gccgatcagg caaccggcct
     1621 cttcctgctc ttcaccggtc atcttccagg ggcgaaccag gtgtccttgg ggaactttcc
     1681 gcaactccgg cacccaacgc cgcacatatt ccccttcggg gtcgaacttt tcctgctggg
     1741 tcatcggatt gaacatgcgc tggaaatagg gggcggggtc ggtcccggtc gaagcgaccc
     1801 actgccagcc gccgttgttc gaggcgacgt cgccgtcgat caggagttcc atgaaatgcc
     1861 tttcgccctc tcgccagtcg aggtggagat ccttggtcaa aaaggaagcg acgac
//
LOCUS       DAOQFI010000419         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_37400_length_1914_cov_3.562668, whole genome shotgun sequence.
ACCESSION   DAOQFI010000419 DAOQFI010000000
VERSION     DAOQFI010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37400_length_1914_cov_3.562668"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1450
                     /locus_tag="PLE93_07620"
     CDS             323..1450
                     /locus_tag="PLE93_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86955.1"
                     /translation="MFNTKNRVVFALLAIFTLSALLAGPAQAKRKHKPKKPNTVKVMS
                     RNLYLGADLGPAINSSDAADFISKNGQILRDVDTNNFPVRARGLAGEIRKVSPDLVGL
                     QEVALWRTGDASFTPSITGEYTASTVKVDFLADLLAQLNKGGVKRYRVVKVQNEFDFE
                     APADYDNDPATGAVTLGGEINGRLTMRDAVLAKVGAGIRTRNIKSGNYEHIFQPVISG
                     IPVKVKRGWLSADVKVRKSKWFRFVNTHFEAFGEPQIREQQAKELLAGPLKSGTKPAV
                     LVGDLNSDDDTVSEDDALAYDALTKGGMVDRGTQKMTFGVDSGIITDDAPTNFNQTID
                     HVMTNRPKSVKLLASGVTGTKPLNGYWDSDHRGVWSNLRIP"
     gene            1609..>1914
                     /locus_tag="PLE93_07625"
     CDS             1609..>1914
                     /locus_tag="PLE93_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNH86956.1"
                     /translation="MGKTIGIDLGTTNSCVAVLEGGEPTVLENSEGGRTTPSVVAFND
                     KDERLVGTVAKRQAVMNPENTIFSIKRFMGRKEAEVKEEETMIPYKVEAGPNGDVRVD
                     "
BASE COUNT          408 a          619 c          571 g          316 t
ORIGIN      
        1 atggcgtggg accggacgat cctgacggac tccggcggct tccaagtctt ctcgatgggg
       61 cacggcacgg tcgccgacga gattgccgac ttcgtcgccc attacgccgg cgaaggttag
      121 ggacaacaag gcgtcatccg gtcttcgccg gatgacaacg ggaacccgcc ggcccgcttg
      181 attcgctgat ggtcgggccg gctccgattg atcagtctcc gcatcgttcg ggcccttgcc
      241 gggtttatcc tcctgacagc ggttggggaa ccgcagaagg cgcgttgccg cgcctcgccc
      301 cagaccaaaa ggagagaact tgatgttcaa caccaagaac cgcgtggtct tcgccctgct
      361 ggcgatcttc acgctttccg cgctgctggc cggcccggcc caggccaagc gcaagcacaa
      421 gccgaagaag ccgaacaccg tcaaggtgat gtcccgcaac ctgtatctgg gagccgacct
      481 cggcccggcg atcaactcgt ccgacgccgc cgacttcatc agcaagaacg gccagatcct
      541 gagggacgtg gacaccaaca acttcccggt ccgggccagg ggcctcgccg gcgaaatcag
      601 gaaggtaagc ccggacctcg tcggccttca ggaagtcgct ctctggcgca ccggcgacgc
      661 cagcttcacg ccctcgatca ccggtgagta cacggcctct accgtcaagg tggacttcct
      721 cgccgacctg ctcgcgcagc tcaacaaggg tggagtcaag cgctaccggg tggtcaaggt
      781 ccagaacgaa ttcgacttcg aggcacccgc cgactacgac aatgatcccg ccaccggcgc
      841 agtcactctc gggggcgaga tcaacggtcg cctgaccatg cgtgacgcgg tcctggccaa
      901 ggtcggtgcc ggcatccgca cccgcaacat caagtccggc aactacgagc acatcttcca
      961 gccggtcatc tccggaatcc cggtcaaggt gaagcgcggc tggctttccg ctgacgtgaa
     1021 ggtccgcaag agcaagtggt tccgcttcgt aaatacgcac ttcgaggcct tcggcgaacc
     1081 gcagatccgc gagcagcagg ccaaagagct gctggccgga ccgctcaaga gcggtacaaa
     1141 gcccgcggtt ctggtcggtg acctgaattc ggatgacgac accgtctccg aggatgacgc
     1201 gctcgcctac gacgccctga ccaagggcgg catggtcgat cgcggcacgc agaagatgac
     1261 cttcggcgtg gacagcggca tcatcaccga cgatgccccg accaacttca accagacgat
     1321 cgatcacgtg atgaccaatc gtccgaagtc ggtcaagctg ctcgcctccg gggtgaccgg
     1381 aaccaagccg ctcaacggct actgggattc cgaccaccgc ggagtctggt cgaacctgcg
     1441 cattccgtag cgcaacgaga caaaccgaat tgccggaaag gcccgcattt gcgggccttt
     1501 cttcgttaat ttgacgttct gtacaaaatc ttatacagtc ccacttagat ttttgaccat
     1561 gaccgcaccc tcatgcggtc tctgacacat ccaaacagga gaaacacagt gggaaagacg
     1621 attggaatcg acctcggcac gacgaactcg tgcgtagcgg ttctcgaagg cggcgaaccg
     1681 accgttctcg agaactccga gggcgggcgc accaccccgt cggtggtcgc cttcaatgac
     1741 aaggacgagc ggctggtcgg caccgtagcc aagcgccagg ccgtgatgaa cccggaaaac
     1801 acgatcttct cgatcaagcg cttcatgggc cgcaaggagg ccgaggtcaa ggaagaggag
     1861 acgatgatcc cgtacaaggt cgaagccggc ccgaacgggg atgtccgcgt ggac
//
LOCUS       DAOQFI010000420         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_37662_length_1907_cov_3.801296, whole genome shotgun sequence.
ACCESSION   DAOQFI010000420 DAOQFI010000000
VERSION     DAOQFI010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37662_length_1907_cov_3.801296"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLE93_07630"
     CDS             <1..185
                     /locus_tag="PLE93_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86957.1"
                     /translation="SLDLFAARARETDAGGLAAWIREWLSGQVDDETLDTYWRAGPFE
                     GMWSGLDRYWQKQEES"
     gene            complement(191..1105)
                     /locus_tag="PLE93_07635"
     CDS             complement(191..1105)
                     /locus_tag="PLE93_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH86958.1"
                     /translation="MSAADLRDWRDRGRMVPATPLGHEAFLIDEGNPEAPPESTVVLL
                     HGFPESSYSYSGAFPLVSGAFDRVVSFDFIGFGFSAKPADGFAYSLIEQADVALRLIR
                     GSGITGCHFVAHDMGDSVMTEILARLNENRPDWFRDGALSVSFTNGSMVLGDAKLRVG
                     QRALVSPAGRMLSRIMDSPKIFEKQVVSAQGNDRLTEEQILDMYSASTSGAPRGLMAE
                     LIGYLRERRRFEKSRWLPAVRDGAMPIQICWGADDAVAPVAIAHRLKTEVKPSAKLTI
                     VDGLGHFAQIQDPEAWFNGLSPFWSEIA"
     gene            complement(1102..1374)
                     /locus_tag="PLE93_07640"
     CDS             complement(1102..1374)
                     /locus_tag="PLE93_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HNH86959.1"
                     /translation="MCRNIRTLYNFEPEATDEEVRAASLQYVRKISGFNKPSRANEEA
                     FDRAVEEIAAVSARLLDSLETSAPPKDRETEERKRRERAAKRFAAA"
     gene            complement(1410..1586)
                     /locus_tag="PLE93_07645"
     CDS             complement(1410..1586)
                     /locus_tag="PLE93_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNH86960.1"
                     /translation="MTDHKTDEAKGRVKEAAGAITDDDELKNEGKADRAASSVKEKVE
                     DAVDTVKEKLAGKD"
     gene            1728..1907
                     /locus_tag="PLE93_07650"
     CDS             1728..1907
                     /locus_tag="PLE93_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH86961.1"
                     /translation="MSDYAVVNPATGETVKEYPTISDAGLQEAIASAEDAFRDWSKNS
                     SIEERVRRSSGPAGK"
BASE COUNT          329 a          623 c          603 g          352 t
ORIGIN      
        1 aatccctcga cctctttgca gcccgggccc gggaaaccga tgccgggggc cttgcggcct
       61 ggatccgcga gtggctatcg gggcaggtcg atgacgaaac gctggacacc tattggcggg
      121 ccgggccctt cgaaggaatg tggtcggggc tcgaccggta ctggcagaag caggaagaga
      181 gctaggtcgc tcaggcgatt tcggaccaga agggagaaag cccgttgaac caggcttcgg
      241 gatcctggat ctgggcgaaa tgtccgagac cgtcgacgat cgtcagcttc gcggaaggtt
      301 tcacctcggt cttcagccgg tgggcgatcg ccaccggggc caccgcatcg tcggctcccc
      361 agcaaatctg gatcggcatc gccccgtccc ggaccgccgg cagccaccgt gacttctcga
      421 aacggcggcg ctcgcgcagg taaccgatca actcggccat cagcccgcgc ggcgcacccg
      481 aggtcgaggc cgaatacatg tcaaggatct gttcttcggt caggcggtcg ttgccctggg
      541 cgcttacgac ctgcttctcg aagatcttcg ggctgtccat gatccgggag agcattcgcc
      601 ccgccggcga gaccagtgcc ctctgcccga cccgcagttt ggcgtctccc agcaccatgc
      661 tgccgttggt gaagctgacg ctgagcgccc cgtcccggaa ccagtccggc cggttctcgt
      721 tcaggcgggc aaggatctcg gtcatcaccg agtcccccat gtcatgcgcc acgaagtggc
      781 agccggtgat gcccgacccg cggatcaggc gcagggcgac atcggcctgc tcgatcagcg
      841 aataggcaaa tccgtcagcc ggcttggccg aaaagccgaa gccgatgaag tcgaacgaga
      901 cgacccgatc gaaggccccg gagaccagcg ggaaggcacc cgaatacgag tacgaggatt
      961 ccgggaagcc gtgcagcagc accacggtcg attcgggcgg ggcttccggg ttcccctcat
     1021 cgatcaggaa ggcctcgtgg cccagtggcg tcgccgggac cattcggcca cggtcacgcc
     1081 agtcccgcag gtcggcggcg ctcacgctgc ggcgaaccgt tttgcggccc gctcccgccg
     1141 cttccgctcc tcggtctcgc ggtccttggg cggtgcggaa gtctcgagcg aatcgagcag
     1201 cctcgccgag acggcggcga tctcttcgac cgcccggtcg aacgcttcct cgttggccct
     1261 ggagggtttg ttgaacccgc tgatcttcct cacgtactgg agggaggcgg cccgcacttc
     1321 ttcgtcagtc gcctcgggtt cgaagttgta aagggtcctg atattccggc acatgccccg
     1381 atcttacgcc ccgccgcggg ggcgtcgggt cagtccttgc cggccagttt ctccttgacc
     1441 gtgtccaccg cgtcttccac cttctccttg accgacgaag cggcccggtc ggccttgcct
     1501 tcgttcttga gctcatcgtc gtcggtgatt gctcccgcgg cttccttcac gcggcccttg
     1561 gcttcgtctg tcttgtgatc ggtcattgct acctccttct gggtttcaac caggtagtac
     1621 cccggtgctc gcgctccaat ccgattcacc cgaaacggcc aaccaatagt ctgcggtgag
     1681 acaccttcag gtgtcagcca aaggcgcaaa tcgaggattg gagattcatg agcgactacg
     1741 cagttgtgaa cccggcaaca ggcgaaaccg tgaaggagta tccgacgatc tcggacgccg
     1801 gcctccagga agcgatcgct tccgccgagg atgccttccg ggactggtcg aagaacagtt
     1861 cgatcgagga acgggttcgg cggtcaagcg gtccagcagg gaagtga
//
LOCUS       DAOQFI010000421         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_37709_length_1906_cov_3.168558, whole genome shotgun sequence.
ACCESSION   DAOQFI010000421 DAOQFI010000000
VERSION     DAOQFI010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37709_length_1906_cov_3.168558"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLE93_07655"
     CDS             complement(<1..310)
                     /locus_tag="PLE93_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNH86962.1"
                     /translation="MPATENEIKEKYLERAIRELNQLNRDLQGCDACLTTKVMPVLGS
                     GHPQADMMLVKFAPTTAEVEEGVAFYGRAGNALMKSFKRLNIDPTVIYGTVCAKCPVE
                     D"
     gene            complement(379..>1906)
                     /locus_tag="PLE93_07660"
     CDS             complement(379..>1906)
                     /locus_tag="PLE93_07660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH86963.1"
                     /translation="TEERPFATQFARPLLEIFFGRLDARTASSKTSAKLLNRYFPGSY
                     EITHPGVDPAEPARVEVPPLKIAFSEREEPGALRLFLRALRRLPADLDWTASIYADDP
                     GEVQLRVASAIRDRIKVIGPEQVSLADLLSHSDVFVAASNGPRPDPAAVRQAIASGAV
                     PVTTTIGRYVELVGDGQRGLLFPAGDPVTLAGQIQRLGEDSRLREGFRAQCGKFEVES
                     WSGVAGRFEAIYERLVSRRHDPAGNEELRKKLRDRPLIEVDLHMHTDHSSDCATPVKV
                     LIETARDRGFGAIAITDHNEVSGAMEAAEVAAEMDDFKIIVAEEVMTGDAGEVIGLFL
                     KEKIPKGTSMSDTIREIKRQGGLVYVPHPFDRLHSVPDYENLLDIVEDIDLMEVFNPR
                     VAISVFNEEAERFARKYNIIPAAGSDSHVAQGLGAVRNRLHDFDGPEEFLEAMREAEI
                     TRRHKNLVYVQALKFIQTTGRPKAAKRQVDDPQAVRGGRRKARGSKQARGPKRAAGKS
                     "
BASE COUNT          295 a          621 c          641 g          349 t
ORIGIN      
        1 gatcctcgac cgggcacttg gcgcagaccg tgccgtagat cacggtcggg tcgatgttga
       61 gtcgtttgaa ggacttcatc agggcgttgc cggcccggcc gtagaacgcc acgccctcct
      121 ccacctcggc ggtggtcggg gcgaacttga ccagcatcat gtcagcctgc gggtgaccgg
      181 agccgagcac cggcatcacc ttcgtggtca ggcaggcgtc acagccctgc agatcacggt
      241 tcagctgatt cagctcccgg atcgcgcgtt ccagatactt ctctttgatc tcgttttcgg
      301 tcgcaggcat cgctgatgcc ttattagacg cccccggcgg ggctcctgcc ggatcccggc
      361 tgtcaccggg tccgtgcatc agctcttgcc tgctgcacgt tttggcccac gcgcctgctt
      421 cgagccacgg gccttgcgcc ggccgcctct gacggcctgc gggtcatcga cctgacgctt
      481 ggccgccttg ggccgcccgg tggtctgaat gaacttcagc gcctgaacgt aaacgaggtt
      541 cttgtggcgg cgggtgatct ccgcctcgcg cattgcttcg aggaactcct cgggcccgtc
      601 gaaatcatgg aggcggttgc gaaccgcccc cagaccctgg gcgacatggc tgtcggagcc
      661 ggccgccggg atgatgttgt acttgcgggc aaaccgttcg gcttcctcgt tgaagacgga
      721 gatcgcgacg cgggggttga agacctccat caggtcgatg tcctcgacga tgtcgagcag
      781 gttctcgtaa tcggggaccg agtggaggcg gtcgaaggga tgcggcacat agacgaggcc
      841 gccctggcgc ttgatctccc tgatcgtgtc cgacatcgag gttcccttcg ggatcttctc
      901 cttgaggaag aggccgatca cctcgccggc gtcaccggtc atcacttcct ccgcgacgat
      961 gatcttgaag tcatccatct ccgccgcgac ctccgcggct tccatggccc cggaaacctc
     1021 gttgtggtcg gtgatcgcga ttgctccgaa gccgcggtcg cgggcggttt cgatcagcac
     1081 cttgaccggg gtcgcgcagt cactcgaatg gtcggtgtgc atgtggaggt cgacctcgat
     1141 cagcggccgg tcgcgcagct tcttcctcag ttcttcgttg ccggccggat catggcggcg
     1201 ggagaccagc cgctcgtaga tcgcctcgaa tcgcccggcc accccggacc agctttcaac
     1261 ctcgaacttg ccgcactgcg cccggaagcc ttcacgcagc cgggaatcct cgcccagccg
     1321 ctggatctgg ccggccagcg tgaccgggtc cccggccggg aagagcaggc cgcgctggcc
     1381 gtccccgacc agttcgacat agcggccgat cgtggtcgtg accggcaccg cgccggaagc
     1441 gatcgcctgg cgaacggccg ccgggtccgg ccgcggaccg ttggaggcag ccacgaagac
     1501 atcggaatgg gagagcaggt ctgcgagcga aacctgttcg ggaccgatca ccttgatccg
     1561 gtcgcgaatg gcgctggcca cacgaagctg gacttcgccg ggatcgtccg cgtagatgct
     1621 ggcggtccag tcgaggtcgg ccggcaggcg ccgcagggcc cgcaggaaga ggcggagcgc
     1681 ccccggttcc tcacgctcgg agaaggcgat cttgagcggt ggcacctcga cccgggccgg
     1741 ctcggccggg tcgaccccgg ggtgggtgat ctcgtatgaa ccggggaagt aacggttgag
     1801 cagcttggcc gaggtcttcg aggatgcggt cctcgcatcg aggcggccga aaaagatctc
     1861 gagcaggggc cgggcgaact gggtcgcgaa gggccgctcc tcggtc
//
LOCUS       DAOQFI010000422         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_37902_length_1901_cov_2.394908, whole genome shotgun sequence.
ACCESSION   DAOQFI010000422 DAOQFI010000000
VERSION     DAOQFI010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37902_length_1901_cov_2.394908"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1901)
                     /locus_tag="PLE93_07665"
     CDS             complement(<1..>1901)
                     /locus_tag="PLE93_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86964.1"
                     /translation="SQASVWLAWVVDRWYPEKLAELEQALGGKGAGKAGRRGPQLAYS
                     DQRLVTEDGQVIAETYWSGRRNNHTNFASMLIANTVTGAASLFTREMLEYALPFPEAP
                     GEQFHDHWLALVAMSTGGIAYVDKPLYDYVQHGSAVLGHDAANAGIKAGRPKRPKHLS
                     IGAVRQFFSGWRSAYFNAYLRLSVLARVILARCDGKLSGRQRRTLKRYVKAEKSPAGF
                     LWLLFRPVRELFGRNETLGVERLLIQGILWRYIIFWRNRGLEKPVGSLYDASLPPAGR
                     AAKAPAHNHPQTAHIQRMVRPLDLAVSEEAPERVNLLIPTFDLKHLFGGYIAKFNLAR
                     KLAEAGFRTRIVTVDPTPPLPRSWREQVESYQGLEGAFENIEIFFARDSGEALEINPR
                     DRFVATTWWTAHLANDALSRTEHDHFLYLIQEYEPLTFVTGSWYAMADATYDFPHRAL
                     FSTELLREFFRDRGYGVYREGAATGDELSVSFQNAITPVSAPPAAELSARGTRRLLFY
                     ARPEAHAARNMFEIGLMSLQQAVEDGVFGPEWSFHGIGTVEGQSRLRLTDEVALEILP
                     RTSQADYGELLAGHDLGLSLMLTPHPSLPPLEMASAGMLTVTNTCENKTADKLTAISP
                     NLIAAPPTVP"
BASE COUNT          300 a          602 c          678 g          321 t
ORIGIN      
        1 ggcacggtgg gcggtgcggc gatcaggttc ggggagatcg cggtcagctt gtccgcggtc
       61 ttgttctcgc aggtgttggt aacggtcagc atcccggccg aagccatctc cagcggaggc
      121 aggctcggat gcggggtcag catcagcgaa aggccgagat catggccggc cagcagctcg
      181 ccgtagtcgg cctggctggt tcggggcagg atctcgaggg cgacctcgtc ggtcagcctg
      241 aggcggctct ggccctcaac cgtgccgatc ccgtgaaagc tccattccgg gccgaagact
      301 ccgtcttcga ccgcctgctg caggctcatc aggccgatct cgaacatgtt gcgcgccgcg
      361 tgggcctcgg gtctcgcgta gaaaagcagc cgacgggtgc cccgggccga gagttcggcc
      421 gcgggcgggg cactgaccgg cgtgatcgcg ttctggaagg agaccgaaag ctcgtccccg
      481 gtggccgctc cctcccgata gaccccgtag ccgcggtcgc ggaagaactc cctgagcagc
      541 tcggtcgaaa agagtgcccg gtgcgggaag tcgtaggtcg catccgccat cgcgtaccag
      601 gagccggtca cgaaggtcag cggttcgtat tcctggatca ggtaaagaaa gtgatcgtgc
      661 tcggtccgcg acagcgcgtc gttggcgagg tgggccgtcc accaggtggt tgcgacgaag
      721 cggtcccgcg gattgatctc gagcgcctcg ccggagtcgc gggcgaagaa gatctcgatg
      781 ttctcgaatg caccttcgag cccctggtag gactcgacct gttcgcgcca ggacctgggt
      841 agcggcgggg tcgggtcgac ggtgacgatg cgggtgcgaa agccggcctc ggccagtttg
      901 cgggccaggt tgaacttggc gatgtagccg ccgaagagat gcttgaggtc aaaggtcggg
      961 atcagcagat tgacccgctc cggcgcctct tcgctgaccg ccagatccag cggccggacc
     1021 atgcgctgga tgtgggcggt ctggggatgg ttgtgggccg gggccttggc ggcccggccg
     1081 gccgggggca ggctggcgtc atagagcgag ccgaccggct tctccagccc ccggtttctc
     1141 cagaagatga tgtagcgcca gaggattccc tggatcagca gccgctcgac cccgagggtt
     1201 tcattccggc cgaagagctc ccggaccggc ctgaagagca gccagagaaa accggccggg
     1261 ctcttctccg ccttgacgta tcgcttcagg gtgcggcgct ggcgaccgct cagcttcccg
     1321 tcgcagcggg ccaggatcac ccgggccagg accgacagcc tgaggtacgc gttgaagtag
     1381 gccgaacgcc agccggagaa gaattgccgg accgcgccga tgctcagatg cttcgggcgc
     1441 ttcggccggc ccgccttgat cccggcgttg gccgcgtcat gtccgagcac ggccgagccg
     1501 tgctggacgt agtcgtagag cggtttgtcc acgtaggcga tgccgccggt ggacatcgcg
     1561 accagggcca gccagtgatc gtgaaactgc tcgcccggcg cctccgggaa aggcagggcg
     1621 tattcgagca tctcccgggt gaagagcgac gcggcaccgg tcaccgtgtt ggcgatcagc
     1681 atcgaggcga agttggtgtg gttgttgcgc cggcccgacc agtaggtctc ggcgatcacc
     1741 tggccgtctt cggtgaccag cctctggtcg ctgtaggcga gctgcgggcc gcggcggcct
     1801 gccttgccgg cgcccttgcc cccgagggcc tgctccagct cggccagctt ctccgggtac
     1861 cagcggtcga ctacccacgc cagccatacc gacgcctgcg a
//
LOCUS       DAOQFI010000423         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_38081_length_1896_cov_2.642586, whole genome shotgun sequence.
ACCESSION   DAOQFI010000423 DAOQFI010000000
VERSION     DAOQFI010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38081_length_1896_cov_2.642586"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1896
                     /locus_tag="PLE93_07670"
     CDS             <1..>1896
                     /locus_tag="PLE93_07670"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH86965.1"
                     /translation="PGDKGDYTTRFTPCPLTYSTIRTFDTSVSPFQEGSNQIRVCSRD
                     YASSEANASKTCSATRTVLIDNRFSNPADNLRSDQGTDWQPVNGFTLRWEIPEGQVAP
                     IIGAVYKIQELETDTLVDAGYFSGNDIQSAGPINLPAVGAYKVIIHLVDGALNLGRDA
                     ETILRFDDRPPGDVSPVEPEGWISRDELPIQQEIEKAAPGGPSGISGYALEVSDDGPA
                     QPCGSEICLAPEITLAGGPDLRTGSIGGLAEGDHWISAAAVSGAHKSSVEPGSTMVQV
                     DRTPPTVSISGVPNEWVNHPVTLTAQASDQLSGMQPQPADDGSPKTVIDADNYAAYES
                     PGPFASFSVATEGVNRVRYWAEDLAGNANDGMPGHDGDTHATPGQAVVRIDTTPPEVR
                     FDPARDPEDPEVVRISADDTDSGVASASIGIRRAGSGETFIQLQTAGADGQYQARIPS
                     DDLAQGAYELNATVEDRAGNESTGNRTSAGDPMIVNLPVKQPASIAASLTGGKSNILA
                     RYGSARYLEGRLTSGETALSNQTVTAIETFKAGSSRNAVSRDLVTDGDGRFRVLLTPG
                     PSRTIEVKYAGTRMISRTAGPKLGMRVKGKVTLKIKPKKVLNGGVVRMSGKVGFAGVL
                     PPSRGKLV"
BASE COUNT          391 a          600 c          609 g          296 t
ORIGIN      
        1 ccgggtgaca agggtgacta cacgaccagg ttcactccct gcccgctgac ttactcgacg
       61 atcaggactt tcgatacctc ggtctcgccc tttcaggaag ggtcaaacca gattcgggtc
      121 tgttctcgtg actacgcgag ctccgaggca aacgcttcaa aaacatgcag cgccaccagg
      181 actgtcctga ttgacaaccg gttctcaaat ccggcggaca atctgcgttc cgatcaggga
      241 accgactggc aacccgtcaa cgggttcacc cttcgctggg agataccgga aggccaggtc
      301 gcaccgatca tcggggccgt ctacaaaatt caggaactcg agaccgacac gctggtcgac
      361 gccggctact tcagcggaaa cgacattcag agcgctggtc cgatcaacct gcccgcggtc
      421 ggcgcgtaca aggtgatcat ccatctggtc gacggagcgc tcaacctcgg acgggacgcc
      481 gagaccatcc tccgcttcga cgaccggccg ccgggtgacg tgagtccggt cgaaccggag
      541 ggatggatct caagggacga actgccgatt cagcaggaga tcgaaaaggc agcgcccggt
      601 ggcccgtccg gaatcagtgg ctacgcgctg gaggtgtccg acgacgggcc ggcgcaaccc
      661 tgcggatccg agatctgcct cgccccggag atcaccctgg ccggaggccc ggacctgagg
      721 acgggttcga tcggcggcct tgccgagggt gatcactgga tttcggcggc tgcggtctcg
      781 ggggctcaca agtcctcggt cgagccgggc agcacgatgg ttcaggtcga ccggactcca
      841 ccgaccgtct cgatatccgg agttccgaac gaatgggtca accatccggt caccctgacc
      901 gcccaggcga gcgaccagct ctccgggatg cagccccagc cggctgacga cggcagtcca
      961 aaaacggtga ttgatgccga caactacgcg gcctacgaat cacccggacc tttcgccagt
     1021 ttctcggtgg cgaccgaagg cgtaaaccgg gtccgctact gggcggaaga tctggccggc
     1081 aacgcgaacg acgggatgcc gggacatgac ggcgacactc acgcgactcc gggccaggcc
     1141 gtggtccgga tcgacaccac cccgccagag gtcagattcg atccagccag ggatccggag
     1201 gatccggaag tcgtccggat ctccgcagat gacaccgact cgggcgtcgc ctctgcttcg
     1261 atcggaatcc ggagggcggg gtcgggagag acattcattc aactccagac cgcgggggcc
     1321 gacggtcagt accaggccag gatcccttcc gatgacctgg cgcaaggcgc gtatgaactg
     1381 aacgcgaccg tcgaggacag ggccggaaac gaatcgaccg gaaaccggac ttcggccggc
     1441 gatccgatga tcgtcaacct acccgtaaag cagcccgcct cgattgccgc gtcgctcact
     1501 ggcggaaagt cgaacatcct cgcgcgctat ggcagcgccc gttacctgga aggcaggttg
     1561 accagcggcg aaacggcgct gtcgaaccag acggtcacgg caatcgaaac gttcaaggcc
     1621 ggttcgagcc ggaacgcggt ttcgagggat ctggttacgg acggagacgg ccgcttcagg
     1681 gtcctcctta ccccgggccc gtcgcggacg atcgaagtca aatacgcggg caccaggatg
     1741 atctcccgga ccgccggacc gaagctcggc atgagggtga aaggcaaggt gacgctgaag
     1801 atcaagccga agaaagtgct caacgggggc gtcgtccgga tgtcgggcaa ggtcggtttt
     1861 gccggggtgc tcccgccgtc ccggggcaag ctggtc
//
LOCUS       DAOQFI010000424         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_38140_length_1894_cov_2.918978, whole genome shotgun sequence.
ACCESSION   DAOQFI010000424 DAOQFI010000000
VERSION     DAOQFI010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38140_length_1894_cov_2.918978"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..718)
                     /locus_tag="PLE93_07675"
     CDS             complement(104..718)
                     /locus_tag="PLE93_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNH86966.1"
                     /translation="MPELPEVETIRRQLEPDLNGRVIETVEVLDPYFTKPDLPGNFER
                     AVNGREIVDVGRRGKYLLLELDDGLTVVIHLRMTGSLVLLAPDGSENPGDARIYEADR
                     SERHLKARFTLDDGGELRFSDPRRFGRGFGGSADTMGSADSTGAVSVTAGDDAEAPHS
                     PLLVPFEPRLAAWDPARRPGDLTAEAARLPFQALLRFVRSRREA"
     gene            complement(880..1845)
                     /gene="xerD"
                     /locus_tag="PLE93_07680"
     CDS             complement(880..1845)
                     /gene="xerD"
                     /locus_tag="PLE93_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNH86967.1"
                     /translation="MPPATVTSVPAPEVLEGARFEGLLLEFLAYLELERGLSRNTLNA
                     YRTDLLQYGDYLRAKGIDPLEIDAAGMSEYVADLIEGKGSGHRLAASTVHRKTAALRS
                     FYKHLRLEDLVGDDPTAGLSTPRRSKKLPNVLNQAEVTRLLAVPAGDSPQAYRDRAIL
                     EVMYACGLRASEVIDLEMTDVDTYEGMLRARGKGSKERIVPLGRQAIYALEGYLLSGR
                     PDLVKGRSEKALFLNFRGRRLTRQGLYKIVQGHAKSAGLEDRMSPHTLRHSFATHLLA
                     GGCDLRAVQEMLGHADVATTQMYTHLTGQQLRDVYYAAHPRAQAN"
BASE COUNT          317 a          619 c          627 g          331 t
ORIGIN      
        1 gagggggtgt agacgccccc tggatcgagc ggcgcccccg ggttgtggct caagcgatcc
       61 gcgacggtcc gcagcgcctc gcggatcgcg gccatgctcg cgctcacgcc tcgcgcctgg
      121 accggacgaa cctgagcagc gcctggaagg gcaggcgcgc cgcctcggcg gtgaggtcgc
      181 ccgggcgccg cgccgggtcc catgccgcga ggcgcggctc gaaggggacc agcagaggcg
      241 agtggggcgc ctcggcgtcg tccccggccg tcacggagac cgcgcccgtc gagtcggcgg
      301 agcccatcgt gtccgcagag ccgccgaagc cccggccgaa gcgtcgcgga tccgaaaagc
      361 gaagctcgcc cccgtcatcc agggtgaagc gggccttgag gtgcctctcg ctccggtcgg
      421 cctcgtagat cctggcgtca cccgggttct cgctgccgtc cggtgccagc aagaccaggg
      481 agccggtcat tcgcagatgg atgaccacgg tcaagccgtc gtcgagttcg agcagcaggt
      541 atttgccgcg ccgccccaca tccacgatct cccgtccgtt cacggcccgc tcgaagttcc
      601 cgggcagatc gggcttggtg aagtaggggt cgagcacctc aaccgtttcg atcacccgcc
      661 cgttcagatc aggctcaagc tggcgacgaa tggtctcgac ttcaggaagc tcgggcaccc
      721 gccggttgta tcagagccct cccctcggca gtgggtcgga tttccggcac aggcaccggt
      781 tttccgaccc actaccgcgc cggtgagtgg ttcgtaaacc aggtgggtcg gattctcggc
      841 gtgtggaccg gaaatccgac ccaccgggga tttggcttct cagttagcct gggctctggg
      901 gtgggcggcg taatagacgt cgcggagctg ctggccggtc aagtgggtgt acatctgggt
      961 cgtggcaaca tcggcgtggc cgagcatctc ctgtaccgcc ctcaggtcac agccaccggc
     1021 gagcaggtgg gtggcgaagg agtggcgcag ggtgtgaggg ctcattcggt cttcgaggcc
     1081 agccgatttc gcatgcccct gcacgatctt gtagagaccc tgcctggtca ggcggcggcc
     1141 ccgaaagttg agaaaaagcg ccttttcgga tcgccccttg accagatccg gccgacccga
     1201 aaggaggtac ccctccagag cgtagatcgc ctgtcgacca agcggcacaa ttctttcctt
     1261 gctgcccttg cccctcgccc gcagcatccc ctcgtaggtg tcgacgtcgg tcatctcgag
     1321 gtcgatcacc tcggaggccc gcagtccgca ggcgtacata acttcgagga tcgcccgatc
     1381 ccggtaagcc tgcggagaat ctcctgccgg aaccgcgaga agtcgtgtca cctcggcctg
     1441 gttgaggaca ttgggcagct tcttcgagcg acgaggtgtg ctcagcccgg cggtcgggtc
     1501 gtccccgacc agatcctcga gtcgcagatg cttgtaaaac gaacgcaggg cggcggtctt
     1561 gcgatgaacg gtcgaagcag cgagtcggtg gccggaacct ttgccctcga tcaggtcggc
     1621 cacgtattcg gacattccgg ctgcgtcgat ctcaagcgga tcgatccctt ttgcccgcag
     1681 atagtcaccg tactgcagca ggtcggtgcg gtaggcattc agggtgttgc gcgaaagacc
     1741 ccgctccagt tcaagatagg cgaggaactc gagcagcagg ccttcgaaac gggcgccttc
     1801 gagtacttcc ggagcaggga cggacgtgac ggttgccggg ggcatgaaag cgctttctcc
     1861 cccacccgct tcatcccttg ccccgcaacg aaac
//
LOCUS       DAOQFI010000425         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_38461_length_1885_cov_3.079235, whole genome shotgun sequence.
ACCESSION   DAOQFI010000425 DAOQFI010000000
VERSION     DAOQFI010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38461_length_1885_cov_3.079235"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLE93_07685"
     CDS             complement(<1..335)
                     /locus_tag="PLE93_07685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNH86968.1"
                     /translation="MANRKEVTNQITSVKNIRQITRAMEMVAAARLRRAETRIQHLRP
                     YAQAMRRMTQRAADQAGAVTQVPILEQRETVKRVALLLVTGDRGLAGAFNANILRDGL
                     NRRDELKAQ"
     gene            complement(380..>1885)
                     /gene="atpA"
                     /locus_tag="PLE93_07690"
     CDS             complement(380..>1885)
                     /gene="atpA"
                     /locus_tag="PLE93_07690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNH86969.1"
                     /translation="DNCMALEMLDLPHGVTGLALNLEADNVGAVLFGEWDKIVEGDTV
                     KRTGRLLEIPVGEEFLGRMVDPLGRPLDGKGDVKSTETRPAEFKAPGVISRQPVREPM
                     QTGIKAIDGMIPIGRGQRELIIGDRQTGKTAVTIDSIINNKDSGVISVYVAIGQRMST
                     IVQVREVLEAAGAMDNTIIVAAPADEAAPIKFIAPYAGTAMAEYFLYKGGHALCIYDD
                     LTKHAYAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAVKLSDAEGGGSLTSLPIIE
                     TQANDVSAYIPTNVISITDGQIFLESDLFNSGVRPAINVGISVSRVGGDAQTKAMKKV
                     AGKMKGELSQYRDLAAFAQFGSELDAETQRTLARGERLVELLKQNERDALSVADQVAS
                     IYSGTGGYLDLIKTERVQDFLVDLLARLHSDNKALLDRINETGQFPEEDEKELGDAIG
                     NFVDDFGPDFDENGEALAEGESVRVDSAFAEEPDSAGKEEVIENEEAPSAA"
BASE COUNT          269 a          597 c          653 g          366 t
ORIGIN      
        1 tactgcgcct tcagctcgtc gcggcggttg agcccgtcac ggaggatgtt ggcgttgaag
       61 gcaccggcca gaccgcggtc accggtgacc aggagcaggg cgacgcgctt gacggtctcc
      121 cgctgctcga ggatcggcac ctgggtgacc gccccggcct ggtcggccgc acgctgcgtc
      181 atgcgccgca tcgcctgtgc gtaggggcgc agatgctgga tccgcgtctc cgcccggcgc
      241 agccgcgcgg cagccaccat ctccatcgcg cgcgtgatct ggcggatgtt cttgacgctg
      301 gtgatctggt ttgtgacttc tttgcggttt gccatctagt gcttccgtat tgccgtgagg
      361 cggaaaggtc cgtttccgcc taggcggcgg agggagcctc ctcgttctcg atgacttctt
      421 ccttgccggc cgagtcgggc tcttcggcga aggccgaatc aacccgtacc gactcgccct
      481 cggcaagcgc ttcgccgttc tcgtcgaaat cgggtccgaa gtcgtcgacg aagttgccga
      541 tcgcgtcgcc cagttccttt tcgtcttctt ccgggaactg gccggtttcg ttgatccggt
      601 cgagcagggc cttgttgtcc gagtggagcc gggccagcag gtcgaccagg aagtcctgga
      661 cgcgctcggt cttgatcagg tcgaggtaac caccggtgcc ggagtagatc gaagcgacct
      721 ggtcggcgac cgaaagggca tcgcgctcgt tctgcttgag cagctcgacc agacgctcgc
      781 cgcgggccag cgtgcgctgg gtttccgcgt cgagttcgga gccgaactgg gcgaaggcgg
      841 cgaggtcgcg gtactgcgac agctcgccct tcatcttgcc ggcgaccttc ttcatcgcct
      901 tggtctgggc gtcaccaccg acccgcgaca ccgagatgcc gacgttgatg gcggggcgga
      961 cgcccgagtt gaagaggtcc gactcgagga agatctggcc gtcggtgatc gagatgacgt
     1021 tggtcgggat gtaggccgac acgtcgttgg cctgggtctc gatgatcggc agcgaggtca
     1081 gcgatccgcc gccctccgcg tcgctcagct tgaccgcccg ttcgagcagg cgcgagtgca
     1141 ggtagaagac gtcgccgggg tacgcctcgc gtcccggggg gcgccggagc agcagcgaca
     1201 tctgacggta cgcgtaggcg tgcttggtca ggtcgtcgta gatgcagaga gcgtggccgc
     1261 ccttgtagag gaagtactcg gccattgccg tgccggcgta gggcgcgatg aacttgatcg
     1321 gggccgcctc gtcagcggga gcggcgacga tgatggtgtt gtccatcgcc ccggccgcct
     1381 cgagcacctc gcggacctgg acgatcgtcg acatccgctg gccgatcgcg acgtagaccg
     1441 agatcactcc cgagtccttg ttgttgatga tcgagtcgat cgtgacggcg gtcttgccgg
     1501 tctggcggtc gccaatgatc agctcgcgct ggccacggcc gatcgggatc atgccgtcga
     1561 tcgccttgat gccggtctgc atcggctcac gcacgggctg gcggctgata acgccgggcg
     1621 ccttgaactc ggccggtcgg gtctcggtcg acttgacgtc acccttgccg tcgagcggac
     1681 ggccgagcgg gtcgaccatg cggccgagga attcctcgcc gaccgggatc tcgagcaggc
     1741 ggccggtgcg cttcaccgtg tcgccttcga cgatcttgtc ccactcgccg aagagcacgg
     1801 cgccgacgtt gtcggcttcg aggttcagcg cgagtccggt taccccgtgg ggcaggtcga
     1861 gcatctccag cgccatgcag ttgtc
//
LOCUS       DAOQFI010000426         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_38464_length_1885_cov_2.903825, whole genome shotgun sequence.
ACCESSION   DAOQFI010000426 DAOQFI010000000
VERSION     DAOQFI010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38464_length_1885_cov_2.903825"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLE93_07695"
     CDS             complement(<1..216)
                     /locus_tag="PLE93_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNH86970.1"
                     /translation="MARKGMGGMPGGMDMNKLLQQAQQMQAEMMQAQESLKDEVVEAS
                     AGGGMVKVKISGDLVVQDVVIDPDAVDP"
     gene            complement(216..>1885)
                     /gene="dnaX"
                     /locus_tag="PLE93_07700"
     CDS             complement(216..>1885)
                     /gene="dnaX"
                     /locus_tag="PLE93_07700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNH86971.1"
                     /translation="QHVVRTLSNAITQDKVHHAYLFVGSRGTGKTSMAKILAASLNCE
                     NGGPTLTPCGTCESCRSIAAGNSVDVIEMDAASNRSVDDIRELRDRVAFAPTAGRWKV
                     YILDEAHMLTKEAWNAFLKTLEEPPPNTVFILATTEAHKVMPTIADRCQRFDFQRPSI
                     EQLSEVLTRVAAAEQITIGAPAISMISRSASGSFRDALGTLDQLVSYSGNEIALDDVL
                     EVLGAADADLLFGVVDKVIEADPAGVLTAVEQMARSGRDPARFARDLLGHLRVLLVTQ
                     TTGQIPEAFAVTAADPDRLVRQAKAIGPANLIRTIDELSAALGLIRDGDEARLAVEVA
                     LLKAARPDFDPSSEGLARRLERLESGVRPVAPPPIQESPPAPSAAPTPPASEAAASSA
                     PPSPPAVAEAEAAAPAVAPEPPKPQAAPTPPEPSAAPTPAPEPEPYDLPKLEQVWPAV
                     LDSLGASASGMVASYFDGTVPVALDGTKLSIGFPADAAFSRRNAERPECRQQLAEAIK
                     VVTGDQFQVEYASLDSGQEVADPESEVMEEEDFIARVKSEFNAEEVI"
BASE COUNT          290 a          596 c          649 g          350 t
ORIGIN      
        1 gggatcaacg gcgtcgggat cgatcaccac gtcctggaca accaggtcgc cgctgatctt
       61 caccttgacc atgccgccgc cggcgctggc ttcgacgact tcgtccttga gtgactcctg
      121 ggcctgcatc atctcggcct gcatctgctg ggcctgctga agcagcttgt tcatgtccat
      181 tccaccgggc ataccgccca ttcccttgcg tgccatcaga tgacctcctc tgcgttgaat
      241 tcggatttga cccgggcgat gaagtcttct tcctccatca cttccgattc cgggtcggcc
      301 acctcctggc cgctgtcaag acttgcgtat tcgacctgaa actgatctcc ggtcacgacc
      361 ttgatcgctt cggccagttg ctggcgacat tcaggccgct cggcgttcct gcggctgaaa
      421 gcggcgtcgg ccggaaagcc gatgctgagc ttcgtgccat ccagggcgac cggcactgtg
      481 ccgtcgaagt aggacgcgac cattccagac gccgaagccc cgagtgaatc cagcacggcc
      541 ggccagactt gctccaactt cggcaggtcg taaggctccg gttctggggc cggtgtcggc
      601 gcagctgatg gttcgggtgg tgttggtgca gcttggggtt tgggtggttc gggcgcgacg
      661 gccggagcgg ctgcctccgc ctctgcgaca gcgggcggcg atggtggcgc tgacgacgca
      721 gctgcctcag acgccggcgg agtcggggcg gcggacggcg ccggcggcga ttcctggatc
      781 ggaggcgggg ccacggggcg gactcccgac tcgagccttt ccagccgtcg cgccaatcct
      841 tcacttgacg gatcgaagtc gggacgggca gctttcagca gcgccacttc caccgcgagt
      901 ctcgcctcgt ccccgtcacg gatcaggccc agggcggcag acagctcgtc gatcgtgcga
      961 atcaggttgg ctggtccgat cgctttcgcc tggcggacca gacggtccgg gtcggcggca
     1021 gtcaccgcga atgcctcggg gatctggccg gttgtctggg tgaccagaag cacccgaagg
     1081 tggcccagga ggtcgcgggc aaagcgggcc gggtcgcgac cggaccgggc catctgctcg
     1141 accgcggtca ggacgccggc cgggtcggcc tcgatcacct tgtcaaccac accgaagagc
     1201 aggtcggcgt cggccgcccc cagcacctcg agcacgtcgt cgagcgcgat ctcgttcccg
     1261 ctgtaggaga cgagctggtc cagagtgccg agcgcgtcgc ggaaacttcc cgaagcggag
     1321 cggctgatca ttgagatcgc cggagcgccg atcgtgatct gctcggcggc cgcgacgcgg
     1381 gtcaacacct cggagagctg ttcgatcgag ggtcgctgga aatcgaagcg ctggcaacgg
     1441 tcggcgatgg tcggcatcac cttgtgggcc tcggtcgtgg cgaggatgaa gaccgtgttc
     1501 ggcggcggtt cctcgagagt cttgaggaag gcgttccagg cctccttggt cagcatgtgc
     1561 gcctcgtcga ggatgtagac cttccagcgg ccggcggtcg gggcgaaggc gacccggtcc
     1621 cgcagttcgc ggatgtcgtc gaccgagcgg ttggaggctg catccatctc gatcacatcg
     1681 accgaattgc cggccgcgat cgagcggcag gattcgcagg tgccgcaggg ggtgagggtc
     1741 ggaccgccgt tctcgcagtt cagcgaggcg gcgaggatct tcgccatcga tgtcttgccg
     1801 gtaccccgtg agccgacgaa caggtaggcg tgatggacct tgtcctgggt gatcgcattg
     1861 gacagggtcc ggaccacatg ttgct
//
LOCUS       DAOQFI010000427         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_38645_length_1881_cov_2.443593, whole genome shotgun sequence.
ACCESSION   DAOQFI010000427 DAOQFI010000000
VERSION     DAOQFI010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38645_length_1881_cov_2.443593"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLE93_07705"
     CDS             complement(<1..622)
                     /locus_tag="PLE93_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HNH86972.1"
                     /translation="MLKLRRATVVSVEPLEVRLEVSDQPRPAWADESMVGPCEAGDEV
                     IVNVEALDLGLGSGGFDVIHVNLTRGLSGEGSATEHVMKLNYTSLQHPVGTIEREEGT
                     GRAPVVPVLAIGLHGHLAPAAWAAAEAGAEAIGYIQTGGGALPGSLSRDVSELLDRGL
                     LAGNVDGGDVAGAVGLMEAGAAVGRRIERHGDAEQGEEVHPDRAPEQ"
     gene            complement(622..1752)
                     /locus_tag="PLE93_07710"
     CDS             complement(622..1752)
                     /locus_tag="PLE93_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH86973.1"
                     /translation="MSRFVRLCETASPTGEERAIADLVRSELELLGLEVHEDDAAGPA
                     GAAAGNLLTRIPGRPREDGTDPGYLMFCAHLDTVPHDDRIEVVLDEHGVYRSAGETIL
                     GADNKAAVAVLIEMAARSTVDAPPVGLELLFTVAEEQGLRGAAAFDQSQLRSTVGFVL
                     DHATDIGEVITAAPTHIGIRAEFHGVEAHAGLIPESGRSAIAAAAGAIAEMKLGRLDM
                     ETTANVGKIAGGTSGNVIPGFCALTGEARSVDPVRVTEVIGEMTDAMIWAASESGIEI
                     DVATERHFAGYRVEDDSRALDLAESALASRGHQPQRITTGGGSDANVFRERGMDCLLL
                     ANGTYANHTSDENVPRENLSEMLEICLAIANCAGDDPARVEA"
BASE COUNT          321 a          667 c          570 g          323 t
ORIGIN      
        1 cctgctctgg ggcgcggtcg gggtgtactt cctcaccctg ttcggcatcg ccgtggcgtt
       61 cgatccggcg acccaccgca gcgccagcct ccatcagacc tacggcccct gccacgtcac
      121 cgccatcgac attgccggcc agaaggcccc ggtcgagcag ttccgacacg tcccgcgaaa
      181 gggacccggg cagcgccccg ccgccggtct ggatgtaccc gatcgcttcc gccccggcct
      241 ccgcagccgc ccaggccgcc ggagccaggt gaccatgcag accgatcgcc aaaaccggaa
      301 ccaccggggc ccgcccggtc ccttcctcgc gctcgatcgt gccgaccgga tgctggagcg
      361 aggtgtagtt cagcttcatc acgtgctcgg tggccgaacc ttctccgctc aggccccggg
      421 tcagattcac gtggatcacg tcgaatccac cagatcccaa tccgagatca agtgcctcga
      481 cgttgacgat cacctcgtcc ccggcctcac agggaccgac catcgattca tcggcccagg
      541 ccgggcgagg ctggtcagag acttcgagcc tgacctcaag tggctctacg gaaaccacag
      601 tggcccggcg cagcttcagc atcaggcttc cacccgggca ggatcgtcac ccgcgcagtt
      661 cgctatggcc aggcagatct ccagcatttc ggaaaggttc tcacggggaa cgttctcgtc
      721 agaggtgtgg ttggcgtatg tgccgttggc gagcagcagg cagtccatgc cgcgctcccg
      781 gaacacgttc gcgtccgagc cgccaccggt cgtgatccgc tgcggttgat ggccgcgact
      841 ggcaagcgcg gattcagcca ggtccaaagc ccttgaatca tcctcgaccc ggtagccggc
      901 gaagtgcctc tccgtcgcca catcgatttc aatcccgctc tcggaggcgg cccagatcat
      961 tgcgtcggtc atctctccga tcacctcggt gacccgcacc gggtcaaccg atcgggcttc
     1021 gccagtcagg gcgcagaaac ctggaatcac gttgcccgag gtgccaccgg cgatctttcc
     1081 gacgttggcg gtcgtttcca tgtcgaggcg accgagtttc atctcagcga ttgccccggc
     1141 cgccgccgcg atcgcggacc gtcccgattc ggggatcagc cccgcatgtg cctcgacccc
     1201 gtgaaactcg gcccggatac cgatatgtgt cggtgctgcg gtgatcactt ccccgatatc
     1261 ggtggcgtga tcgaggacga agccgaccgt ggaccgaagt tgcgactggt cgaacgcggc
     1321 ggccccccgc agaccctgct cctcggccac cgtgaaaagc aattcgagac cgaccggcgg
     1381 cgcatcgacc gtggaccggg cggccatctc gatcagcacc gcgaccgccg ccttgttgtc
     1441 ggcgccgaga atcgtttccc cagccgagcg gtacaccccg tgttcatcta gcacgacctc
     1501 gatccgatcg tcatgtggca ccgtgtcgag gtgggcgcag aacatcaggt agccggggtc
     1561 ggtcccgtcc tcacgcggcc gtccgggaat ccgggtcaac aggttgccgg cagcggcacc
     1621 ggccggaccg gccgcatcat cttcatgaac ctcgagtccg agcagttcga gttcgctgcg
     1681 aaccagatcg gcaatcgccc gctcttcccc ggtcgggctg gctgtttcgc agagtcggac
     1741 aaaacggctc actcgagccg aatcatctca cgacgcgaac gcagcaaccg ggaccgaagc
     1801 cggccagccc gtcagtgaac gctgctcgcc ggcgggtttc cgttgtcctt tagcgggggc
     1861 tggaaggcga tcagatacag c
//
LOCUS       DAOQFI010000428         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_38809_length_1877_cov_2.725576, whole genome shotgun sequence.
ACCESSION   DAOQFI010000428 DAOQFI010000000
VERSION     DAOQFI010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38809_length_1877_cov_2.725576"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLE93_07715"
     CDS             complement(<1..369)
                     /locus_tag="PLE93_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86974.1"
                     /translation="MSEPLNNNSESGEEPRVKLPPSEPRGYFEGESMTRRHVFTVASQ
                     AIGGVAAAAVVLPVVGFAVAPLFERGEERWEAVGPLSDFNAETYKQVVITETPGIGEA
                     GKTSAYVRTGAEKYNEEPNDV"
     gene            complement(374..952)
                     /locus_tag="PLE93_07720"
     CDS             complement(374..952)
                     /locus_tag="PLE93_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86975.1"
                     /translation="MSPSSALDRLADRVREEGSPLTVREPGQASAFGELAAAGPRTSE
                     RAGDYAFVVEAVREGYLCHYGESRILEMPDPDLALLAGDLFYAIGIRGLAELDDLEST
                     GILSDLIRVAAELEAAGRPDLAETLWVAQIVALACGKDDQLSSATAALEAGQTDAARA
                     VREWSIETAAANGLGREFDLAQTAIDSRPSNF"
     gene            complement(949..>1425)
                     /locus_tag="PLE93_07725"
     CDS             complement(949..>1425)
                     /locus_tag="PLE93_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH86976.1"
                     /translation="PAAAVGGSRELMERLAPTGDVYQAGTLSGNPLAVSAGLATLRRL
                     DDQAYSRLGVLTDRLVTGLKDLADEHGLTVASAPGLVTLFFSALPVKDFEDAKATDRE
                     RHAAFYRAMLERGIWLPPSQFEAWFVSLAHDEIMIDRTLMAAAEALAATDPRRAVV"
     assembly_gap    1426..1435
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1436..1663)
                     /locus_tag="PLE93_07730"
     CDS             complement(<1436..1663)
                     /locus_tag="PLE93_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH86977.1"
                     /translation="MSREAKDHPFIPTLKSYLSEGRLDRREFLRQATARAGAMLVFDE
                     VITGFRVARGGAQELFGIVPDLTVMGKIIGGG"
BASE COUNT          307 a          646 c          613 g          301 t
ORIGIN      
        1 cacgtcgttc ggctcctcgt tgtacttctc ggcgccggtg cggacgtagg cggaggtctt
       61 gccggcctcg ccgatgcccg gggtctcggt gatcacgacc tgcttgtagg tctcggcgtt
      121 gaagtcactg agcgggccaa ccgcttccca gcgttcctcg ccgcgctcga agagcggcgc
      181 gaccgcgaac ccgaccacgg gcagcacgac ggccgccgcg gccaccccac cgatcgcctg
      241 gctggcgacg gtgaagacat gccgacgggt catcgactcg ccctcgaagt aaccacgggg
      301 ctcactcggg ggcagtttca cgcggggctc ttcccccgac tcggaattgt tgttcaacgg
      361 ttcggacacg cttctagaaa ttcgacgggc gggagtctat tgcagtctgc gccaagtcga
      421 attcccgccc cagcccgttg gcagccgctg tttcaattga ccactccctg accgccctgg
      481 cggcgtccgt ctgacccgcc tccagggccg ccgtcgcgga actcagctgg tcgtcttttc
      541 cgcatgccaa tgcgacaatc tgggctaccc acaaggtctc cgccaggtcc ggccggccgg
      601 ccgcctcaag ctcggccgcg acccggatca aatccgacag gattcctgtg gattcgaggt
      661 cgtcgagttc ggcgagcccg cgaatgccga tcgcatagaa gagatctccg gccaggagcg
      721 cgagatccgg gtcgggcatc tcgaggatgc gcgactcgcc gtagtggcag aggtaaccct
      781 cgcgaaccgc ctcgaccacg aaggcgtaat cgccggcccg ttccgaggtt cgggggcccg
      841 cggccgcgag ctcaccgaag gccgaagcct gcccgggctc cctgaccgtc aggggtgatc
      901 cttcctcgcg tacccggtcg gcgagccggt cgagggcact cgacggactc aaacgacggc
      961 cctgcgagga tcggtggcag ccagggcttc cgcggctgcc atcagcgtgc ggtcgatcat
     1021 gatctcgtcg tgggcgagcg agacgaacca ggcctcgaac tgtgacggcg gcagccagat
     1081 cccgcgttcc agcatcgccc ggtagaaggc ggcgtgccgt tcacggtcgg tcgccttcgc
     1141 atcctcgaag tccttgaccg gcagggccga gaagaagagc gtgaccagtc ccggcgcgga
     1201 ggccacggtg aggccatgct cgtcggcgag gtctttcagg cccgtgacca gccggtcggt
     1261 caggacgccg agccggctgt aagcctggtc gtcaaggcgt cgcagggtgg ccagtcccgc
     1321 ggacaccgcc aacgggtttc cggaaagggt gcccgcctgg tagacgtcac cggtcggggc
     1381 gagcctctcc atcagctcgc gcgagccgcc gaccgcggcg gcaggnnnnn nnnnnaccgc
     1441 cgccgatgat cttgcccatc acggtcaggt cgggaacgat cccgaacagc tcctgggcgc
     1501 cgccgcgggc cacccggaac ccggtgatca cctcatcgaa gaccagcatc gccccggccc
     1561 gggcggtcgc ctgccgcagg aattcgcgcc ggtccaggcg gccctcggaa aggtaggact
     1621 tcagcgttgg aatgaacgga tggtctttcg cttccctgct catcgatacc ccctgttgcc
     1681 acggatcgac gccgacgcaa gcggcgcatc cgccttgtcc ccgaaactgg aagctaagca
     1741 cgcagccgcg cgtcccacta gatggaatgc ggcagggatt ccagaattgg cggcgtcgga
     1801 acgcgcctgt cgcgcgatcc tctccatcaa tgcacgaggc ttgcgggcgt caggcgtcgg
     1861 aatgcgtgat cgcaacc
//
LOCUS       DAOQFI010000429         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_38834_length_1876_cov_3.725975, whole genome shotgun sequence.
ACCESSION   DAOQFI010000429 DAOQFI010000000
VERSION     DAOQFI010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38834_length_1876_cov_3.725975"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLE93_07735"
     CDS             <1..304
                     /locus_tag="PLE93_07735"
                     /inference="COORDINATES: protein motif:HMM:NF017013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HNH86978.1"
                     /translation="EPGLGGPNQEAAIAASAELEGRQAAAVFLDTDGSDGSTRFAGAI
                     CDGRTAERARELGLDLESALASHAASEPLRALEDLIDTGPTGTNVNDLFVAVTGPI"
     gene            complement(329..622)
                     /locus_tag="PLE93_07740"
     CDS             complement(329..622)
                     /locus_tag="PLE93_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86979.1"
                     /translation="MSEPEAGEGDESTEVEQSPVPARGERFGFDLGNPVERESAAFSW
                     LIVVIIAAVAVGAVGKLVSPLAAVILVLILLGIVSVPIFRGLRHGLGSPDDDE"
     gene            complement(630..1256)
                     /locus_tag="PLE93_07745"
     CDS             complement(630..1256)
                     /locus_tag="PLE93_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HNH86980.1"
                     /translation="MESASIAAHGHVDDHHHGPPEAHQSARIDRTILGILLFILSEVM
                     LFGAFFAAYFFLRVVADPASWPPEGVELPVLIAGINTCILVSSSFTVHYALEAIKHNN
                     HRGLKLGLTTTWLLGATFLFIQINEYIHIGFTPRDGAFGSIFFSLTGLHGAHVFVGLL
                     LLTFANIRAYRGHFGPEQKDHLGVEVPGIYWHFVDIMWIIVFLAVYII"
     gene            complement(1259..>1876)
                     /locus_tag="PLE93_07750"
     CDS             complement(1259..>1876)
                     /locus_tag="PLE93_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86981.1"
                     /translation="GEVLLSCTYDFRFGLTRRDLLSWAEITYGDVTNITHIPVRIEDD
                     SISLNVTHTLVVANRTLTSEDLQERLLDRSGDRPHRYTIIAPAADDVSEATVARNLAT
                     VMAKLYHADIDATGQPMSPDPFDAVKNAIEHYRVDEILISTLSGEESHWLKEDLIGRV
                     REITEKPVVHHEAGRPPEAVAAVVAEGEAPEAAGAEDSTAEENEA"
BASE COUNT          334 a          576 c          661 g          305 t
ORIGIN      
        1 ggagccgggg cttggtggac ccaaccagga agcggcgatc gccgcttccg ccgagcttga
       61 aggccgtcag gctgccgccg tcttcctcga cacggacggc tccgatggca gcacccgctt
      121 cgcgggcgcg atctgcgatg gcagaaccgc cgaacgcgcc cgcgaactcg gcctcgacct
      181 cgagtccgca ctggcctcac acgccgcctc ggagccgctg agggcccttg aggacctgat
      241 tgacaccgga cccaccggca ccaacgtcaa cgacctcttc gtcgcggtca cgggcccgat
      301 ctgaaccagc cgggctgacg gagcggcgct actcgtcgtc gtcggggctt ccgaggccgt
      361 gcctgaggcc gcggaagatc ggcacgctaa cgatgcccag gaggatcagc acgaggatca
      421 ccgcggcgag gggcgagacc agcttgccga ccgctcccac cgctaccgcg gcgatgatga
      481 ccacgatcag ccagctgaat gcggcactct cccgctcgac cggattgccg agatcgaaac
      541 cgaaccgctc accccgggcg ggcaccggcg actgctccac ctcggtggat tcgtcccctt
      601 ccccggcctc gggctcgctc atggccgggt cagatgatgt agacggcgag gaacacgatg
      661 atccacatga tgtcgacgaa gtgccagtag atgcctggca cctcgacacc gaggtggtcc
      721 ttctgttccg gaccgaagtg gccgcggtag gcgcgaatgt tggcgaaggt caagagcagc
      781 aggccaacga agacatgggc gccgtggagc ccggtgaggc tgaagaagat cgaaccgaag
      841 gcaccgtcac gcggcgtgaa gccgatgtgg atgtactcgt tgatctggat gaagaggaag
      901 gtcgcgccga ggagccaggt cgtggtcagg ccgagcttca gaccgcgatg gttgttgtgc
      961 ttgatcgcct cgagcgcgta atggaccgtg aacgaggagg aaaccaggat gcaggtgttg
     1021 atgccggcga tcaggaccgg gagctcgacg ccctccggcg gccaggaagc cggatcggcg
     1081 acgacccgca ggaagaagta ggcggcgaag aaggcgccga acagcatcac ctcggaaagg
     1141 atgaaaagca ggatgccgag gatcgtgcgg tcgatgcggg cgctctgatg cgcctcgggc
     1201 gggccgtgat ggtggtcgtc gacgtgaccg tgggcggcga tgcttgcgct ttccatctct
     1261 atgcctcgtt ctcttccgcg gttgaatctt cagcgccagc ggcttcggga gcttcgccct
     1321 cggcgaccac ggcagcgacg gcctccggcg ggcggccggc ctcgtggtgg accacgggct
     1381 tctcggtgat ctcgcgcacc cggccgatca ggtcttcctt cagccagtgg gactcttcac
     1441 cgctcagggt cgaaatcagg atctcgtcga cccggtaatg ctcgatcgcg ttcttcacgg
     1501 cgtcgaacgg gtccgggctc atcggctggc cggtggcgtc gatgtcggcg tggtagagct
     1561 tggccatgac ggtcgccagg ttccgggcaa cggtggcctc ggaaacatcg tcggcggccg
     1621 gagcgatgat cgtgtaccgg tggggacggt cgccggagcg gtcgagcaga cgctcctgaa
     1681 ggtcctcgga ggtcagggtc cggttggcga cgaccagggt gtgggttacg ttcaggctga
     1741 tcgagtcgtc ctcgatgcga accggaatgt gggtgatgtt ggtgacgtcg ccgtaggtga
     1801 tctcggccca ggagagcagg tcgcgcctgg tcaggccgaa acggaagtca taggtgcagg
     1861 agagcagaac ctcgcc
//
LOCUS       DAOQFI010000430         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39092_length_1870_cov_3.415427, whole genome shotgun sequence.
ACCESSION   DAOQFI010000430 DAOQFI010000000
VERSION     DAOQFI010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39092_length_1870_cov_3.415427"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLE93_07755"
     CDS             complement(<1..218)
                     /locus_tag="PLE93_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH86982.1"
                     /translation="MADYIFTMYQVSRVHPPDKEVLKDISLNFLPGAKIGILGPNGSG
                     KSSLLKIMAGIDTEFKGEARLREGATVG"
     gene            complement(250..1584)
                     /locus_tag="PLE93_07760"
     CDS             complement(250..1584)
                     /locus_tag="PLE93_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86983.1"
                     /translation="MERQVPNSGQWRMCLPVIALLAALLALAAAPALALADGKPDLSF
                     GKKGTSAFDPDPLSLQSPSDMVTDSKGRIVMGGTTFNGPGGNMAWFTRLNRNGSVDST
                     FGTGGFTTFPVPAQTYIATVAVDSEGRIVAAGNYDLPMNRDIWVLRLKSDGHLDSGFG
                     IGGGVGFDFGSFDLGYAAVVGKDGLIYVAASTGDGDSDKATVLRYTSDGDPDSTWGTG
                     GAVRLEVGGTSSRAEAIRLDRRGRVVLGGERVNPDDSSDFLAIRLTRKGALDKTFNGR
                     GFRTIRFMKGQRAEVSDMRVDGKGRILLLGAGDIAPDGLPDGLLARLTPNGKPDKSFG
                     LSGKVVKSIFGTRMYLAHVRIDRAGRIVTAGYGRDPRNAEQALLIRFESNGRSDRSWG
                     RNGVRKLTFGQPEGEGTAMTIDGKGRYLVGGYVFDTGLSYVTRLLVKYPRHR"
     gene            1677..>1870
                     /locus_tag="PLE93_07765"
     CDS             1677..>1870
                     /locus_tag="PLE93_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86984.1"
                     /translation="MEVDLNFYLQSIVAVVVITDPLTRGIFFRQLTANEPERRGEFVR
                     RITIVVAVTLFGAALVGRELL"
BASE COUNT          324 a          628 c          576 g          342 t
ORIGIN      
        1 atgccgaccg tggcgccttc gcgcagccgc gcttcaccct tgaactcggt gtcgatgccg
       61 gccatgatct tgagcagcga ggacttgccg gaaccgttcg ggccgaggat gccgatcttg
      121 gcgcccggca ggaagttcag cgaaatgtct ttgaggacct ccttgtcggg cggatggacc
      181 cgggagacct ggtacatggt gaagatgtag tcagccacgg agggcaccct accgggcggc
      241 cggtgcgtcc taccggtggc gcgggtactt gacgagaagt cgcgttacat atgacaggcc
      301 cgtgtcgaaa acgtacccgc cgaccaggta ccggcccttg ccgtcgatcg tcatcgcagt
      361 cccttcacct tcgggctggc caaaggtgag ctttctcacc ccgttcctgc cccagcttcg
      421 gtcggagcgc ccattcgatt cgaagcgaat gagcaacgcc tgctcggcat ttctcgggtc
      481 cctcccgtaa cccgccgtga cgatccggcc cgcccggtcg atcctgacgt gagccagata
      541 catgcgggtg ccgaagatgc tcttcacgac cttgccgctg agtccgaagc tcttgtcggg
      601 cttgccattc ggcgtgagcc tggccaacaa cccgtccgga agtccgtcgg gagcgatgtc
      661 gcccgccccg agcaggagga tccggccctt tccgtcgacc ctcatgtcgc tgacttcggc
      721 ccgctggccc ttcatgaacc tgatggtcct gaaccctctc ccgttgaagg tcttgtccag
      781 ggcccctttg cgggtgagcc ggatcgccag gaagtccgag ctgtcatcgg gattgacgcg
      841 ctcgccgccc aggacgaccc tgccacgccg gtcgagcctg atcgcttccg cccgggagga
      901 ggtaccaccg acttccagcc ggacggcacc accggttccc caggtggaat cgggatcccc
      961 atccgatgtg tagcggagca ccgtggcctt gtccgagtcg ccgtcaccgg tcgaagcggc
     1021 cacgtagatc aagccgtcct tgccgaccac cgcggcgtaa ccgaggtcga aactgccaaa
     1081 gtcgaaaccg actccgccgc cgatgccgaa gcctgagtcg aggtgcccgt ccgatttcag
     1141 acgcagcacc cagatgtcac gattcatcgg aaggtcatag tttcccgcgg ccacgatcct
     1201 gccctcggaa tcgaccgcga ccgttgcgat gtacgtctgc gctggaaccg gaaacgttgt
     1261 gaaaccgccg gttccgaagg tcgagtcgac gctgccgttc cggttcagcc gggtgaacca
     1321 ggccatgttc ccgcccggtc cgttgaaggt ggtcccgccc ataacgatcc gacccttcga
     1381 gtcggtgacc atgtccgaag gactctggag cgaaagcggg tcgggatcga acgcagaggt
     1441 ccccttcttg ccgaaggaca ggtcgggctt cccatccgcg agcgcgaggg cgggcgctgc
     1501 cgcaagagcc agaagcgcag ccagcaacgc aatgaccgga aggcacatgc gccactgacc
     1561 tgaattggga acctgccgtt ccattctcca cctcacttga actgtcccca acctatcccc
     1621 gacccacgcg gatggcaacc cgggcgaccg ggtcgctctt taccctgcgg gcagggatgg
     1681 aggtcgatct caacttctac ctgcagtcga tcgtcgcggt ggtggtgatc accgatcccc
     1741 tgacgcgggg gatcttcttt cgccagctga ccgcgaacga gcccgagcgc cggggcgagt
     1801 tcgtccggcg aatcacgatc gtggtcgccg tgacactttt cggagcggcg ctggtcggcc
     1861 gtgagctgct
//
LOCUS       DAOQFI010000431         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39093_length_1870_cov_3.401653, whole genome shotgun sequence.
ACCESSION   DAOQFI010000431 DAOQFI010000000
VERSION     DAOQFI010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39093_length_1870_cov_3.401653"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLE93_07770"
     CDS             complement(<1..275)
                     /locus_tag="PLE93_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86985.1"
                     /translation="MNQTGRVQGKDGRERRRLPSPALVLSLLALFVALGGTATALKGK
                     WSVKNDDIAPRAVDAGKVKLGSINGHHLADGVVGPWKLKLGASAVTSG"
     gene            complement(495..>1870)
                     /locus_tag="PLE93_07775"
     CDS             complement(495..>1870)
                     /locus_tag="PLE93_07775"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HNH86986.1"
                     /translation="REVDKDAVYLLTEKLALPLKPMPPNFKNHGNYVTSVSKLSRFLA
                     EKAEEMGAYILTETTADKLLVEDQIVRGIRSGDRGRGRQGQELGNFEPGMDLIAKGTV
                     LAEGVVGHLTYAALDHFDINGLDPQRFELGVKEIWEVEEPLDQVIHTMGWPLRKAAKY
                     KEFGGSFIYPMEDNKICIGFVAGLDYTDATFSVHDVLQQFKQHPFVKKILKGGKRVGW
                     GAKTIPQGGYWSMPKRLSVPGMVIAGDNAGMVNVAELKGVHYAMHAGMYAAEAIVDAL
                     EKDQVDFSAYDEKVHNSIIEKDLYKTRNASQPFTRGFFFGGAMASTMTLTQGHFPGGH
                     WKNHDDATYKMEIGKASESYPEPDGEYTFDKLSSVFLSGNATRDDAPNHVRIQTEVPR
                     EIAQTWVSMCPAQVYEIPEDQLESDAPIVDVHVTASNCVQCGAITAKGGRLTLPEGGD
                     GPLYTET"
BASE COUNT          300 a          589 c          618 g          363 t
ORIGIN      
        1 ccggaggtga ccgcggacgc tcccagtttg agcttccagg ggccaaccac tccgtcggcg
       61 agatggtggc cgttgatcga gcccagcttg accttgccgg cgtccacggc ccttggcgcg
      121 atgtcatcgt ttttcaccga ccatttgccc ttgagcgcgg tggcggtacc gccgagcgcc
      181 acgaacaaag cgagcagcga gaggaccagc gccggcgatg gcaacctgcg cctttcgcgt
      241 ccgtccttcc cctgcaccct gcctgtctga ttcatcatcc ctccctcggg aatcgtgagt
      301 gttggttcac agctacccgg gcgggaccgg atgaaaccgg catcctcagg cggcgcaccc
      361 gaactggcac caaagagaag cgacgccgga tttctccggc gtcgccttga actgcttcgg
      421 gccgtaaagc ccgggcgctt gatctccagg ccggagacca gccgtgacgg ctgaccccct
      481 gtccggcctc tggatcaggt ctcggtgtag agcgggccgt cgccgccttc cggcagggtc
      541 aggcggccgc ccttggccgt gatcgcgccg cactggacgc agtttgaagc ggtgacgtgg
      601 acgtccacga tcggagcatc cgactcgagc tggtcctcgg ggatctcgta gacctgcgcc
      661 gggcacatcg aaacccaggt ctgggcgatc tcgcgcggga cctcggtctg gatccggacg
      721 tgattcggcg cgtcgtcgcg ggtcgcgttg cccgagagga agaccgacga cagcttgtcg
      781 aaggtgtact cgccatccgg ctccgggtag ctctcggaag ccttgccgat ctccatcttg
      841 taggtggcgt catcgtggtt cttccagtga ccgccgggga agtggccctg ggtgagggtc
      901 atggtcgaag ccatggcgcc accgaagaag aagccccggg tgaacggctg gctcgcgtta
      961 cgggtcttgt agaggtcctt ctcgatgatc gagttgtgga ccttctcgtc gtaggcggag
     1021 aagtcgacct gatccttttc gagggcgtcg acgatcgctt cggcggcgta catgccggcg
     1081 tgcatcgcgt aatggacacc cttgagctcg gccacgttga ccatgcccgc gttgtcgccg
     1141 gcaatgacca tgcccgggac cgaaagacgc ttcggcatcg accagtaacc gccctgcggg
     1201 atcgtcttgg cgccccagcc gacccgcttg ccccccttga ggatcttctt cacgaaggga
     1261 tgctgcttga actgctgcag gacgtcgtgg accgagaagg tggcgtcggt gtaatcgaga
     1321 ccggcgacga agccgatgca gatcttgttg tcttccatcg ggtagatgaa cgaaccgccg
     1381 aactccttgt acttggcggc cttgcggagc ggccagccca tcgtgtggat gacctggtcg
     1441 agcggctcct cgacttccca gatctccttg acaccgagct cgaagcgctg cggatcgagg
     1501 ccgttgatgt cgaagtgatc gagcgcggca taggtgaggt ggccgaccac gccttcggcg
     1561 aggacggtgc ccttggcgat caggtccatg cccggctcga agttgccgag ctcctggccc
     1621 tggcggccgc ggccgcggtc accggaacgg attccgcgca cgatctggtc ctcgaccagc
     1681 agcttgtcgg cggtggtctc ggtcaggatg taggcgccca tctcctccgc cttttcggcg
     1741 aggaagcggg aaagcttgga aacggaggtg acgtagttgc cgtggttctt gaagttcggc
     1801 ggcatcggct tgagcggaag cgcgagcttc tcggtcagca ggtagaccgc gtccttgtcc
     1861 acctcccggt
//
LOCUS       DAOQFI010000432         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39249_length_1866_cov_4.050801, whole genome shotgun sequence.
ACCESSION   DAOQFI010000432 DAOQFI010000000
VERSION     DAOQFI010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39249_length_1866_cov_4.050801"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLE93_07780"
     CDS             complement(<1..437)
                     /locus_tag="PLE93_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86987.1"
                     /translation="MREPTYRTRRQGNKRLVVLVVVAAGLLVSGILLMAGGSDDPVAK
                     QLVPPLSTEQTAGEAKPSPEQLRNQSAAIDGAPGPKSKVTGDHDDGLTAPQPTEATNS
                     GSITSTAKRFAELMINWPTDQLEAAANNKELLTLATGTLAGDLA"
     gene            complement(434..>1866)
                     /locus_tag="PLE93_07785"
     CDS             complement(434..>1866)
                     /locus_tag="PLE93_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH86988.1"
                     /translation="LAKQGQRGGGVADHEARPFGNWMSDHAVDILTPENTQVLAVGDG
                     VIYKQSGSARNPNANPAGWTLYLKSGGNYFSYMHLNRFFVHPGDHVKQGDVIGLSGIA
                     NGVAHLHFASTPINPETIVDGTSAAGGSGVVDVETLHNKFGMPKDLPEIYIAAAEKYG
                     LGVRGPSILAGINKVETDFGRLATATSSAGAVGWMQFMPATWAEYGVDGDGDGVKDPV
                     NKWDAIFAAANYLKASGAPGNWHDAIFAYNHAEWYVDEVLGYANQYFPYLQPGGGTGA
                     SCSGELETGPANIQEAITLTEPRKWVPMPPDIGDADEVIDARIVNAARWITQTYHLHI
                     TQGGWQPGSPSVSHGWGTALDMVPTGNYYNSQAGWNQTAMRLAKDLGWTPDCAISGVK
                     PACDLVPAIYDVFYNGFPDHGDPWHNSGGAPHIHIQFECACSGSPPGGQGVASWVKTF
                     PVDGSVSTTTSQPQSDRPRKQKGNKS"
BASE COUNT          285 a          539 c          613 g          429 t
ORIGIN      
        1 gcaaggtcgc cggccagggt gccggtcgca agggtaagga gttccttgtt gtttgctgcc
       61 gcctcgagct ggtcggttgg ccagttgatc atcagctccg caaaccgctt cgcagttgac
      121 gtgatgctgc cggagttcgt ggcttcggtt ggttgcggag cggtcaggcc gtcgtcgtgg
      181 tcaccggtca ccttgctttt cggtccggga gcaccgtcga tcgccgcgga ctggtttcga
      241 agctgctccg gtgaaggttt cgcttccccg gccgtctgtt cggttgaaag aggtggcacc
      301 agctgcttgg cgaccggatc atccgagcca ccggccatga gcagaatgcc gctgaccaga
      361 agccctgcgg ccactacgac caggacaacg agacgcttgt ttccttgccg gcgggttctg
      421 taggtgggtt ctctcatgac ttgttgcctt tctgtttacg gggtcggtcg gactgtggct
      481 ggctggttgt ggtgctcacc gagccatcaa ccgggaaggt cttcacccag ctggcgacgc
      541 cttgacctcc cggcggggaa ccggagcagg cacattcgaa ctggatatgg atgtgcggtg
      601 ccccaccgga gttgtgccag ggatcgccgt gatcagggaa gccgttgtag aaaacgtcgt
      661 agatcgccgg aacgagatcg caggctggtt tcacgccgct tatcgcgcag tccggggtcc
      721 agccaagatc cttcgccagg cgcatcgcgg tctgattcca gcctgcctgg ctgttgtagt
      781 agttgccggt cggcaccatg tcaagggcgg ttccccaacc gtgcgaaacc gacggggatc
      841 cgggctgcca gccgccttgg gtgatgtgta gatggtaggt ctgggtgatc cacctggcgg
      901 cgttcacgat ccgggcgtcg atcacttcgt cggcgtcacc gatatcgggt ggcatcggca
      961 cccatttgcg aggttcggtc agggtgattg cttcctgaat gttcgccggg cctgtttcaa
     1021 gttcgccgct gcaagatgct ccggttccgc ctcccggttg caggtacggg aagtactggt
     1081 tggcgtagcc gagaacctcg tcgacatacc attcggcatg gttgtaggcg aagatcgcgt
     1141 catgccagtt gccgggtgcc ccggacgctt tcaggtagtt ggcggcggcg aagatcgcat
     1201 cccatttgtt gaccgggtct ttgacaccgt cgccgtcgcc gtcaaccccg tattcggccc
     1261 aggttgcggg catgaactgc atccagccga ccgccccggc cgatgaggtt gcggttgcga
     1321 ggcgtccgaa gtcggtttcg accttgttga ttccggcaag gatcgatggt cctctcacgc
     1381 cgagcccgta tttttctgcg gcggcaatgt agatctccgg gaggtctttc ggcattccga
     1441 acttgttgtg gagcgtttcc acgtccacaa ccccggatcc gcctgctgcg cttgtgccgt
     1501 caacgatcgt ttccgggttg atcggggtgg atgcgaagtg aaggtgcgcc accccgttgg
     1561 ctatgcccga tagtccgatc acgtcccctt gtttcacatg gtcgccggga tgaacgaaga
     1621 acctgttgag gtgcatgtag ctgaagtagt tgccaccgga tttcaggtag agcgtccagc
     1681 cggccgggtt cgcgttcggg tttctggcgc tacccgattg cttgtagatg accccgtcac
     1741 cgaccgcgag aacctgggtg ttttcggggg tgagaatgtc gacggcgtgg tcgctcatcc
     1801 agttgccgaa gggtcgggct tcatggtcgg ctacccctcc cccgcgctgg ccctgtttgg
     1861 cgagcg
//
LOCUS       DAOQFI010000433         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39258_length_1866_cov_3.135837, whole genome shotgun sequence.
ACCESSION   DAOQFI010000433 DAOQFI010000000
VERSION     DAOQFI010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39258_length_1866_cov_3.135837"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PLE93_07790"
     CDS             <1..1111
                     /locus_tag="PLE93_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH86989.1"
                     /translation="KESLADGRRAIKAITAVGITSATDAAADPGTLKVWKGLASKGQM
                     PIRLNSFVVVDAGQGVHGAAAYYKAMEKKYSTERLRVPGVKMFMDGVIEYPAQTAALI
                     DPYLEKVGDEWVPGSNRGDIYFTQKQADRFVTRFDRMNRRIHVHAIGDRAAKVALNAA
                     AAARKANRSARRDNIAIAHLQLVDPSDYGRFAKLGVYADMQLQWAVSDFWTEDALHPF
                     IGDERYNRLYPARSLVKAGAPFAMGSDWPVDPLNPWNEIMTANTRRNDYGGLLLANQS
                     IPVATALRAHTLGSARQLGISGKVGSVTVGKDADLIVINRNPLKARPGSIDKTRVLRT
                     IIRGRTVYTPGPGSSALISRAASLSTGEAGHDHGH"
     gene            complement(1196..>1866)
                     /locus_tag="PLE93_07795"
     CDS             complement(1196..>1866)
                     /locus_tag="PLE93_07795"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH86990.1"
                     /translation="AFRLLKSGMTIHGRRIEIIRALRELQNPRIPDEGRRDLAARDAI
                     LDSTVELVLSEGWEALTIEGIANRARVSKQTIYRWWKSKGAVLMDAVLANGFKWPAIP
                     DTGDFEADLKGGLRSLVGEFSDASFDSLLRALLIGAQEDGKLSAELDRQVRSWITAEF
                     AFLIESARQEGRIEAHADPELIIELLYGAVFTRWLLRTGSLDFRFADAVAEMTARALT
                     PGRS"
BASE COUNT          359 a          644 c          563 g          300 t
ORIGIN      
        1 caaggaatcc ctcgccgatg gccgccgggc gattaaggca atcaccgcag tcggcattac
       61 ctcggccacc gacgccgccg ccgacccggg caccctgaag gtgtggaagg gcctggccag
      121 caagggccag atgccgatcc gcctcaattc cttcgttgtg gtcgatgccg gccagggcgt
      181 acacggagcc gccgcctact acaaggcgat ggagaagaag tactccaccg aacgcctccg
      241 ggtgcccggc gtgaagatgt tcatggacgg agtgatcgag tatcccgccc agaccgccgc
      301 cctgatcgat ccctacctcg agaaggtcgg tgacgaatgg gttccgggca gcaaccgcgg
      361 cgacatctac ttcacgcaga agcaggccga ccggttcgtg acccgcttcg accggatgaa
      421 ccggcggatc cacgttcacg cgatcggcga ccgggcggca aaggttgccc tgaacgccgc
      481 cgccgcggcc cgcaaggcca atcgttccgc ccgccgggac aacatcgcga tcgcccacct
      541 ccagctggtc gacccgtccg attacggacg cttcgcaaag ctcggtgtct acgccgacat
      601 gcagctgcag tgggcggtaa gcgacttctg gaccgaagac gccctgcatc cgttcatcgg
      661 agacgagcgc tacaaccgcc tctaccccgc ccgcagcctg gtcaaggcag gggcgccgtt
      721 cgcgatgggc agcgactggc cggttgatcc gctcaatccc tggaacgaga tcatgaccgc
      781 caacacccgc aggaatgatt acggcggact gttgctggcc aaccagtcga ttccggtggc
      841 caccgccctg cgggctcaca ccctgggctc ggcccggcag ctcggcattt ccggcaaggt
      901 cggttcggtc accgtgggca aggacgcgga cctgatcgtg atcaatcgca atccgctcaa
      961 ggccaggccg ggcagcatcg acaagacccg ggtcctcagg acgatcatcc ggggcaggac
     1021 cgtctacacg ccgggccccg gttcatccgc cctgatctcc agggccgcca gcctcagcac
     1081 cggcgaagcc ggccacgacc acggccacta acccgaccag gtcctgaccc gaccagggcc
     1141 tgagccgacc gcggcctgag tcgaccaggt cctgagtcga ccagatccgt ttggttcatg
     1201 accgcccggg tgtcaaagcc cgggcggtca tctccgccac cgcgtccgcg aacctgaagt
     1261 cgaggctgcc ggtccgcagc agccatcgcg tgaacacggc cccgtagagc aattcgatta
     1321 tcagctccgg gtccgcgtga gcttcgattc gcccctcctg gcgggccgac tcgatcaaga
     1381 acgcaaattc agcggtgatc caggaccgaa cctgccggtc aagttcagcg gagagcttcc
     1441 cgtcctcctg ggcgccgatc aacaaggcac gcagcaggga atcgaagctg gcgtcggaga
     1501 actcaccgac caggctacgc aggcctcctt tgaggtcggc ttcgaaatca cccgtatccg
     1561 gaatcgccgg ccacttgaag ccattcgcga gtacggcatc catcagcacg gcgccctttg
     1621 acttccacca ccggtagatg gtttgtttgg atacccgtgc ccggttggcg atgccttcga
     1681 tcgtcagagc ctcccagcct tcggaaagaa cgagctccac cgtggagtcg agaatcgcgt
     1741 cacgggcagc aaggtcgcgt cggccttcgt cggggatccg cgggttctga agttcgcgaa
     1801 gtgccctgat gatctcgatt ctccgaccgt ggattgtcat cccggacttc agtaaccgaa
     1861 acgcaa
//
LOCUS       DAOQFI010000434         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39330_length_1864_cov_3.838032, whole genome shotgun sequence.
ACCESSION   DAOQFI010000434 DAOQFI010000000
VERSION     DAOQFI010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39330_length_1864_cov_3.838032"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLE93_07800"
     CDS             complement(<1..421)
                     /locus_tag="PLE93_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86991.1"
                     /translation="MRRIRFAIAGAAMLCAAAILPAAASADCNLPTGNGGSNNTALSG
                     GTTQTFSGHFGPDLNGKFIQIPFDVSSGITGMKIRYCYAKATGGEADDSPTLDLGVYD
                     AKPAEATTWTQAERRGWSGSAVKTIGIGENGYSPEAVY"
     gene            complement(456..>1864)
                     /locus_tag="PLE93_07805"
     CDS             complement(456..>1864)
                     /locus_tag="PLE93_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNH86992.1"
                     /translation="QAFSQGAFIRHQDGTPYTFNSFVGSGVTQLGMLDFTNPAAQPIY
                     AGILDRAYTAGYDGWMEDYGEYAPPDSVSFDGIPGKRLHNLHPVFYHRGGLRYSNSKK
                     RPIVSFVRSGFTGSARYSQIVWGGDPTTGWGFDGLQSQVTEALTMGLSGVSNWGSDIG
                     GFFSFSPQKLDAELLARWIEFGAFSGVMRSKAEGIGASMDSRPQIWEPETLPVWRRYA
                     KLRTQLYPYLAAANATYRRTGMPVMRHLALTNPTDRRATGIDDQYMFGPSLLVSPVLA
                     PGTGARAVYLPKGKWVDFWQAVSYDEADGSFNLGRAKLLGGGHEVKASAPLEEIPVMA
                     KAGTLLPLLPANTDTLAPYARAAYTGLDDNRGKLHLIALPRGKSSAGYLEHGRISSSE
                     GKRSWKLTVKGAGQVKLDLDASLKTLKHPFRPGSVKAGGKRLKKGKAWSYDRKTGVLH
                     VNTKLKAGQTLSVSAANR"
BASE COUNT          268 a          582 c          678 g          336 t
ORIGIN      
        1 tgtagaccgc ttccggggag tagccgttct cgccgatacc gatcgtcttg accgcgctgc
       61 cgctccagcc gcgccgctcg gcctgggtcc aggtggtcgc ttcggccggc ttcgcgtcgt
      121 agacgccgag gtcgagggtc ggggagtcat ccgcctcgcc gccggtggct ttcgcgtagc
      181 agtagcggat cttcatgccg gtgattccgc tgctcacatc gaaggggatc tggatgaact
      241 tgccgttcag gtccggcccg aagtggccgg aaaaagtctg ggtggtgccg ccggacaggg
      301 cggtgttgtt gctgcccccg ttgccggtcg gcaggttgca gtcagccgaa gcggcggcgg
      361 gcaggatggc ggccgcgcac agcatggcgg caccggcaat tgcgaatcgg attctcctca
      421 ttggctcagg ttattcctgt cggggtcggc ggtggtcatc ggttcgcggc cgagacgctg
      481 agcgtctggc cggctttcag tttcgtgttc acgtggagca cgccggtctt ccggtcgtaa
      541 ctccacgcct tgcccttctt caggcgtttt ccgccggcct tgaccgaacc cggccggaag
      601 gggtgcttca gggtcttcag cgaagcgtcg aggtcgagtt tgacctggcc ggcgcccttc
      661 accgtgagct tccagcttct cttgccctcg ctcgagctga tccggccgtg ctcgaggtag
      721 ccggccgaac tcttgccgcg cggcagggcg atcaggtgga gcttgccgcg attgtcgtcg
      781 aggcccgtgt aggccgccct ggcatagggc gccagggtgt cagtgttggc cggcagcagc
      841 ggcagcagcg ttcccgcctt cgccatcacg gggatctcct ccagcggggc gctggccttg
      901 acctcgtggc cgccgccgag cagcttcgcc cggccgaggt tgaagctgcc gtcggcctcg
      961 tcgtaggaga ccgcctgcca gaagtcgacc cacttgccct tcggcaggta gaccgcacgg
     1021 gcgccggtgc cgggggcgag gaccggcgag acgagcaggc tgggcccgaa catgtactgg
     1081 tcgtcgatcc cggtcgcccg tcggtcggtc gggttggtca gcgcaaggtg gcgcatgacc
     1141 ggcatcccgg tccggcggta ggtcgcgttg gcggccgcca ggtacgggta gagctgagtg
     1201 cgaagcttgg cgtagcggcg ccagacgggc agcgtttccg gctcccagat ctgcggccgg
     1261 gagtccatcg aagcgccgat gccttccgcc ttcgagcgca tcactccgga gaaggccccg
     1321 aactcgatcc agcgggcgag cagttcggcg tcgagtttct gcggcgagaa gctgaagaag
     1381 ccgccgatgt cggagcccca gttggagacg cccgaaaggc ccattgtcag ggcttccgtg
     1441 acctgcgact ggagcccgtc gaagccccag cctgtggtcg ggtcgccgcc ccagacgatc
     1501 tgcgagtagc gggccgagcc ggtgaagccg gaacgcacga agctcacgat cggccgcttc
     1561 ttcgagttcg agtagcgcag gccgccccgg tggtagaaga ccgggtggag gttgtgcagg
     1621 cgcttgccgg ggatcccgtc gaaggaaacc gagtccggcg gggcgtactc gccgtaatcc
     1681 tccatccagc cgtcgtagcc ggcggtgtag gcgcgatcga ggatgccggc gtaaatcggc
     1741 tgggcggccg gattggtgaa gtcgagcatg ccgagctggg tcaccccgct gccgacgaag
     1801 gaattgaagg tgtagggggt gccgtcctgg tggcggatga aggcgccctg cgagaaggcc
     1861 tgat
//
LOCUS       DAOQFI010000435         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39354_length_1864_cov_2.010503, whole genome shotgun sequence.
ACCESSION   DAOQFI010000435 DAOQFI010000000
VERSION     DAOQFI010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39354_length_1864_cov_2.010503"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLE93_07810"
     CDS             <1..628
                     /locus_tag="PLE93_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNH86993.1"
                     /translation="SSKLMIWVAVERASRLAAEFGVKENADRLRAKAAEIKEEIIEKG
                     CRDGVFRQHYETDALDASVLLMPLVRFLPPDDPRIRDTVLAIRDGLDDRGLILRYITD
                     ETDDGLHGEEGTFLICSFWMVAALSEIGEKSAAKKLCERLLAASGDLDLYAEELEASS
                     NRHLVNFPQAFTHLALINAVSHVIADERRVDSAPGTPTAVFTEIREMD"
     gene            673..1368
                     /locus_tag="PLE93_07815"
     CDS             673..1368
                     /locus_tag="PLE93_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNH86994.1"
                     /translation="MRSNVARVRERMAAACERAGREPDSVRLLLATKTVEPARIRVAI
                     EAGETLVAENRVQEVRPKFEALEDLRYERHFIGHLQSNKINALVPYISCLETLDRLSL
                     ARKLQNRLAGEGETLEVLVQVNTSGEPTKAGLDPDEVEPFIRTIAGLDALKVRGLMTI
                     GLNAEPEVARPSLASLREIAGRIREENIEGVAMEELSMGMTGDLEVAIEEGSTIVRVG
                     SAIFGSRPPLQSP"
     gene            complement(1463..1597)
                     /locus_tag="PLE93_07820"
     CDS             complement(1463..1597)
                     /locus_tag="PLE93_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86995.1"
                     /translation="MLIVIGIVLALLVNWALGIICIVIGLVLLIWPAVTGRGSSGSRV
                     "
     gene            complement(1669..>1864)
                     /locus_tag="PLE93_07825"
     CDS             complement(1669..>1864)
                     /locus_tag="PLE93_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86996.1"
                     /translation="DRARRPAAPALRAGPRVAGLHRVVSLALPDNLASRRVMEKAGLQ
                     YVAEIEHVGLPHLLFELDLG"
BASE COUNT          341 a          616 c          617 g          290 t
ORIGIN      
        1 ctcgtcgaag ctgatgatct gggtggcggt ggagcgggcc tcgcgccttg ccgccgagtt
       61 cggggtcaag gagaacgccg accggctgcg cgcgaaggcg gccgaaatca aggaagagat
      121 catcgaaaag ggctgcaggg acggcgtctt ccgccagcat tacgagaccg acgcgctcga
      181 cgcctcggtc ctgctgatgc ccctggtccg cttcctgccc ccggatgacc cgcggatccg
      241 cgacacggtc ctggcgatcc gggacgggct cgacgatcgg ggcctgatcc tccgctacat
      301 caccgatgaa accgacgacg ggctccacgg cgaagaagga accttcctga tctgctcctt
      361 ctggatggtc gcggcgctct ccgagatcgg cgagaagtcc gccgcgaaga agctctgcga
      421 acggttgttg gccgcctccg gcgatctcga cctctacgcc gaggagctgg aggcttccag
      481 caaccgccac ctcgtaaact ttccccaggc tttcacgcat ctggccctga tcaacgccgt
      541 gtcccacgtg atcgccgacg aacggcgtgt cgattcggct ccgggtaccc cgaccgccgt
      601 cttcaccgag atccgggaga tggactgatc gaactcgacc cgggaagcgt cgatccggct
      661 gacgccgagc tgatccgctc gaacgtcgcc cgggtccgcg agaggatggc ggcagcctgc
      721 gagcgggccg ggcgcgagcc ggattcggtg cgcctgctgc tggccacgaa gacggtcgag
      781 ccggcgcgga tccgcgtggc gatcgaagcc ggtgagaccc tggtcgccga gaaccgggtc
      841 caggaggtcc ggcccaagtt cgaagcactt gaagaccttc ggtacgagcg ccacttcatc
      901 ggccacctcc agtccaacaa gatcaacgcg ctggtccctt acatcagctg cctggagacc
      961 ctggaccggc tctcgctggc ccgcaaactc cagaaccggc tcgcggggga aggcgagacc
     1021 ctggaggtgc tggtccaggt caacacctcg ggcgagccga ccaaggccgg gctggatccg
     1081 gatgaggtcg aaccgttcat tcgtacgatc gccggactcg acgcgctgaa ggtccgcggt
     1141 ttgatgacga tcggcctcaa tgcggagccg gaggtggccc gcccttcgct tgccagcctg
     1201 cgggagatcg ccggacggat ccgcgaagag aacatcgaag gcgtggcgat ggaagaactc
     1261 tcgatgggca tgaccggcga cctcgaagtg gcgatcgagg agggctcgac catcgtccgg
     1321 gtgggcagcg caatcttcgg ctcccgcccc ccgcttcaat caccctgagg ctcagcgacc
     1381 gacgaatgcg aagggccccg gcttcccggg gcccttcggt caaaatcggc tgtgcctgat
     1441 cggccgggcg ctacccggcg agtcagaccc ttgagccact gcttcctctt cctgtgaccg
     1501 cgggccagat caacaggacc agcccgatca cgatgcagat gatgcccagc gcccagttga
     1561 ccagcagggc caggacaatt ccgatgacta tgagcaacag tgctagtggc atgcctggga
     1621 ggtacccgga acggcgcccg gcaaacccgc gggccactcg aatgcgggct acccgaggtc
     1681 cagctcgaag agcaggtgcg gcaggccgac gtgctcgatc tcggcgacgt actggagccc
     1741 ggccttttcc ataactcttc gcgaggcaag gttgtccggc agggccaggg agacgaccct
     1801 gtggaggccg gctacccgag gtccagctcg aagagcaggt gcggcaggcc gacgtgctcg
     1861 atct
//
LOCUS       DAOQFI010000436         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39420_length_1862_cov_3.385722, whole genome shotgun sequence.
ACCESSION   DAOQFI010000436 DAOQFI010000000
VERSION     DAOQFI010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39420_length_1862_cov_3.385722"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..>1862
                     /locus_tag="PLE93_07830"
     CDS             164..>1862
                     /locus_tag="PLE93_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH86997.1"
                     /translation="MTTGNQKTRGILIALALLLGALMMFGPARAEAAGYPNISMSKTT
                     SGSVLLGGNSNVTLTASNPNGQPTGYNLSFRDVLPPGISYVPGSSPSSTGEPQVIANA
                     PAAGQTTLIWSNVADLTPNSTFTFTFQVTNSPAVFDIGDTYQNTAGSYLNCDPRYVPK
                     FNAQGQPTQSGGNATCSGLPQETSYTGSATASANTLIKAIEIDKSEPSPEGQLLRGLH
                     DHQTVYTLTVTNNEVDADDNVKVEDYLPAGLEFLGCGTQDNTTDAPTNPGSAEEYPGS
                     GAINPGNAPAAPDCVVPDTVETVLNPSGLPAGVYTHVVWNNLGNLGPSGQLRIQYVAA
                     VPIRQNTMTWTNGVPPVTGTQGSNLDNNSGPETTHLQGLTNHAKVTSDHQGPGGPTEV
                     TDSEDHTVQAVDIRIVKSVTPAGLAVGQISTWSLAIATSEYRYFEDVEVSDTLGDGYC
                     PLGPVNYEHTPPPATAECNPTGNNPSVPYTSAVEQTNGTWQITWDSSTAPALGTINPN
                     SNLTISFPTKTREYFQQNYVNSTPVLSNDTGRNDVDIAGAAWVICAPGAPSPCPNLSP
                     "
BASE COUNT          384 a          670 c          538 g          270 t
ORIGIN      
        1 ccggtacggg gaagggatcg acttcctgaa cgaacacggg atcaccgaga tcgcgacttt
       61 ccgcgaccgg gttcggacga cggcaccgct gggtaaccct aaatagaggc gttcaggaaa
      121 cggattgttc agaaaaacag atcagggacg gaacactggc gaaatgacga cggggaatca
      181 gaagacacgg ggaatactga tcgcgctggc tctcctgctc ggcgccctga tgatgttcgg
      241 tccggcccgg gccgaggccg ccggctaccc gaacatctcg atgtccaaga ccaccagcgg
      301 atccgttctg ctcgggggta actcgaacgt cacccttacc gcctcgaacc cgaacggcca
      361 gccgaccggc tacaacctca gctttcggga cgttctcccg ccggggatca gttacgtccc
      421 cggttcctca ccgtccagca ccggcgagcc acaggtgatc gcgaacgccc ccgctgccgg
      481 gcagaccacg ctgatctggt caaacgtcgc cgatctcacc cccaattcga ccttcacctt
      541 cacttttcag gtcaccaaca gcccggcggt cttcgacatc ggcgacacct accagaacac
      601 ggccggcagc tatctgaact gtgacccgcg ttacgtgccg aagttcaacg cccagggcca
      661 gccgacccag agcggcggaa acgccacctg ttcaggcctg cctcaggaga cttcgtacac
      721 gggctcggcg accgcttcgg ccaacacgct gatcaaggcg atcgagatcg acaagtccga
      781 gccctccccg gaggggcagc tgctccgggg tctgcacgac caccagaccg tctacacgct
      841 gaccgtcacc aacaacgagg tcgatgccga cgacaacgtg aaggtcgagg attacctgcc
      901 ggccggcctc gagttcctcg gttgtggcac ccaggacaac acgaccgacg cgccgaccaa
      961 cccgggcagc gcggaggagt accccggctc gggcgcgatc aacccgggca acgctcctgc
     1021 cgcgccggat tgcgtggtcc ccgacaccgt cgaaacggtg ctcaacccct caggcttgcc
     1081 ggccggggtc tacacgcatg tggtctggaa caacctcggc aacctggggc cttccggcca
     1141 gctccggatc cagtacgtgg cggcagtgcc gatccggcag aacaccatga cctggaccaa
     1201 cggtgttcct ccggtaaccg ggacgcaggg ttccaacctc gacaacaatt cgggtcccga
     1261 aaccacccac ctccaggggc tgaccaacca cgcgaaggtg acctccgacc atcagggccc
     1321 gggcggcccg accgaggtca ctgactccga ggaccacacc gttcaggcgg ttgacatcag
     1381 gatcgtcaag tcggtcacgc cggccggtct ggcggtcggc cagatcagca cctggtcgct
     1441 ggcgatcgcg accagcgagt accgctactt cgaggatgtc gaggtcagcg acacgctcgg
     1501 cgacggctac tgcccgctgg gcccggtcaa ctacgagcac accccgccgc cggcaaccgc
     1561 cgagtgcaac ccgaccggca acaacccgtc ggttccctac acctcggcgg tcgagcagac
     1621 caacggcacc tggcagatca cctgggattc gagcaccgct cccgcactcg ggacgatcaa
     1681 tccgaacagc aacctgacga tcagcttccc gacgaagacc cgcgagtact tccagcagaa
     1741 ctacgtgaat tcaaccccgg tcctctccaa cgacaccggc cggaacgacg tagacatagc
     1801 cggggccgcc tgggtcatct gtgcccccgg tgccccctcg ccctgcccga acctgagccc
     1861 gg
//
LOCUS       DAOQFI010000437         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39473_length_1861_cov_3.104651, whole genome shotgun sequence.
ACCESSION   DAOQFI010000437 DAOQFI010000000
VERSION     DAOQFI010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39473_length_1861_cov_3.104651"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLE93_07835"
     CDS             <1..677
                     /locus_tag="PLE93_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH86998.1"
                     /translation="RSIALVGHGAAGKTSLAALLARLYDPTGGSVSIDGADLREVDLP
                     SLRHQIAYVSDDSFLFSDTIAENISYARPDATRDEIERAARLAQASEFIEALPDGYET
                     VVGERGLTLSGGQRQRVAIARALLADPRILILDDATSSLDATTEQQIRAGLAEVMAGR
                     TTFVIGHRLSTISLADEVLLLEHGRIQDRGTVAELTERSPLFRELTGGSGEEPLFVPE
                     DEEVLA"
     gene            674..>1861
                     /locus_tag="PLE93_07840"
     CDS             674..>1861
                     /locus_tag="PLE93_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH86999.1"
                     /translation="MSPALRNRKSRSAGRNREHREIVAGRGRKLHWTFGLLKPYRTRI
                     IFMVMAVIASTAAALAPPYLAGRAIDSGIEAGNIPALNLTIALFVAAIIVNAVTSWLQ
                     TWLVGWVGIRVLQDLREQVFKHIQRMSIGFFNRNRPGALISRMTNDIEALNQLISDGI
                     VTLFANLLTLFGVVAIMLLLDWRLALITFLVLPLLLVTSLVFRKYSAGAFRETREQIA
                     RVTSHLQETLSGVRVVRAFAQEERHISTMTALNEANRQANMKTVYLNASYFPATELLT
                     AVGTSAILAFGGWQAIEGNLMIGVVISFVGYLQLFFDPIQQLAQLYATYQQGMAALDK
                     IFTLLDTEVEIVDSPEAIDPPPLEGRIRFDDLSFTYEDPEVEAESGEPVTAAPAAPRW
                     AVRD"
BASE COUNT          298 a          675 c          565 g          323 t
ORIGIN      
        1 tccgctccat tgccctcgtt ggccatggtg ctgccggcaa gaccagcctc gccgcactgc
       61 tcgcccggct ctatgacccg accggcggca gcgtttcgat tgacggcgcc gacctccgcg
      121 aggttgacct cccgtcgctt cgccaccaga tcgcctacgt ctccgatgac agtttcctgt
      181 tcagcgacac gatcgccgag aacatctcct acgcccggcc tgacgcgacg cgggacgaga
      241 ttgagcgggc agcccggctc gcccaggcga gcgagttcat cgaggctctg ccggatggct
      301 acgagacggt ggtcggtgaa cgcggtctca ccctttccgg cggccagcgc cagcgggtgg
      361 cgatcgcccg ggcgctgctg gccgacccgc gcatcctgat cctcgatgac gcaacctcct
      421 cgcttgacgc caccaccgag cagcagattc gcgccgggct ggccgaggtc atggccggcc
      481 gcaccacctt tgtgatcggc caccgcctct ccaccatttc cctggccgac gaggtcctgc
      541 tgctggagca cggccggatt caggaccgcg gcacggttgc ggagttgacc gaacgctcgc
      601 cgctcttccg cgaactcacc ggcggctcgg gcgaggagcc gctcttcgtt cccgaagacg
      661 aggaggtgct ggcatgagcc cggcccttcg aaaccggaaa tcgcgaagcg ccggtcggaa
      721 ccgcgagcat cgggaaatcg tcgccggccg gggccgcaag ctccactgga ctttcggcct
      781 gctgaagccg taccggaccc ggatcatctt catggtcatg gccgtgatcg cctcgaccgc
      841 cgccgctctg gcaccgccgt acctggcggg ccgggctatc gactccggca tcgaggcggg
      901 caacatcccg gcgctgaacc tgacgatcgc cctcttcgtc gccgcgatca tcgtcaacgc
      961 agtcacttcc tggctgcaga cctggctggt cggctgggtc gggatccggg tcctgcagga
     1021 tctccgcgag caggtcttca aacacatcca gcgcatgtcg atcggattct tcaaccggaa
     1081 ccggcccggt gccctgattt cccgcatgac caatgacatc gaggcgctga accagctgat
     1141 aagcgacggc atcgtgaccc tgtttgcgaa cctgctcact ttgttcgggg tggtcgcgat
     1201 catgctgctg ctggactggc ggctggccct gatcaccttc ctggtcctgc cgctcctgct
     1261 ggtcaccagc ctggtgttcc gcaagtactc ggccggcgcc ttccgcgaga cccgcgagca
     1321 gatcgcccgg gtcacctcgc acctgcagga aaccctgagc ggggtccggg tggtccgcgc
     1381 cttcgcccag gaagagcgtc acatcagcac gatgaccgcc ctgaacgaag ccaaccgcca
     1441 ggccaacatg aagacggtct atctcaacgc gagctacttc ccggcgaccg agctcctgac
     1501 cgcggtcggc acctcggcaa tcctcgcctt cggcggctgg caggcgatcg agggcaacct
     1561 gatgatcggc gtggtcatct cctttgtcgg ttacctgcag ctcttcttcg acccgatcca
     1621 gcagctcgcc cagctctacg ccacctatca gcagggcatg gccgccctcg acaagatctt
     1681 caccctgctc gacaccgagg tcgagatcgt cgattccccc gaggcgatcg acccgccccc
     1741 gctcgaaggc cggatccgct tcgacgatct ctcgttcacc tacgaggatc ccgaggtgga
     1801 ggcggaatcg ggcgaaccgg tcacggccgc accggccgca ccgcgctggg cggtccgcga
     1861 c
//
LOCUS       DAOQFI010000438         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39511_length_1860_cov_3.322438, whole genome shotgun sequence.
ACCESSION   DAOQFI010000438 DAOQFI010000000
VERSION     DAOQFI010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39511_length_1860_cov_3.322438"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLE93_07845"
     CDS             complement(<1..388)
                     /locus_tag="PLE93_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87000.1"
                     /translation="MSDSGEIAEGEELAVGAPDPADQAPALHSRENVRSVRVKAPTRG
                     NRKLEKFLDAVNRDDEVKAWWYMAQTHAERLGMSDHSWVHMQIVLNIALRLTRLLDKG
                     GVHGAMVTDHGMSARDTEVVVAGGALL"
     gene            complement(385..1197)
                     /locus_tag="PLE93_07850"
     CDS             complement(385..1197)
                     /locus_tag="PLE93_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87001.1"
                     /translation="MSSGSTRRIVERSHKVTAGIDGLDRISALIMSEAEFARDQGAGR
                     VEVIAVPGLRGSRLIRLLDRVADAVGAGPIRIPTRRDWTAATFLGVTVPAGDSLPDPV
                     AVAVIGETAIGFAVGRPGEIPEWNGSRPVGASTMTRKARFQDPPRPGQIEAAVIGASR
                     GIASMFPPPTERVLVVSSLAPVVERLCGPRIGAAEARRGLDAILGQTSDDISAWFGAE
                     APLARHLPGIIVGHAALAEGLGVRVEPALCDLAASRYWLAEREEPVAGRESS"
     gene            complement(1330..>1860)
                     /locus_tag="PLE93_07855"
     CDS             complement(1330..>1860)
                     /locus_tag="PLE93_07855"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HNH87002.1"
                     /translation="EIILTTRNSILILALIAALIIAGNLAFDRSKDGSGTSGADIALA
                     AVTSETPVGVAFQIGDLVQQSRAEAAERIVERQQAEVQRKLEARQQQEKFGDLPGSVT
                     METLEAIADCESGGDPKIVSSSGLYFGKYQFSPGTWESVGGKGLPSDAPEAEQDYRAA
                     LLYERSGPGQWPVCGQ"
BASE COUNT          312 a          636 c          618 g          294 t
ORIGIN      
        1 ggagcagggc tccgcccgcc accaccactt cggtgtcacg ggcgctcatc ccgtggtcgg
       61 tgaccatcgc tccatgcacc ccgcccttgt cgagcagccg ggtcaggcgc agggcgatgt
      121 tgagcactat ctgcatgtgg acccaggagt ggtcggacat gccgagccgc tcggcatggg
      181 tctgggccat gtaccaccag gccttgacct cgtcatcccg gttgacggcg tcgaggaact
      241 tctccagctt gcggttgccg cgggtcggcg ccttgacccg gaccgacctg acgttctcgc
      301 ggctgtgcaa cgccggggcc tgatcagccg gatcgggtgc gccgaccgcc agctcctccc
      361 cttctgcgat ctcccccgaa tcgctcaaga cgattcccgg ccagcgaccg gttcctcacg
      421 ctcggccagc cagtagcggc ttgccgcgag gtcgcaaagg gcaggctcga cccggactcc
      481 gagcccttcg gccagtgccg cgtgaccgac gatgatgcct ggcagatgcc gggccagggg
      541 ggcttcggcg ccgaaccagg cggaaatgtc atcgcttgtc tggccgagaa tcgcgtccag
      601 tccccggcgg gcctccgcgg cgccgatccg ggggccgcaa agtcgctcga ccacgggggc
      661 gaggctcgag acgaccagca cccgctcggt cgggggcggg aacatcgagg cgatcccgcg
      721 ggaggcgccg atcacggccg cttcgatctg gccgggccgg ggcggatcct gaaaccgggc
      781 cttgcgggtc atggtcgagg cgccgaccgg ccgtgagccg ttccattccg ggatctctcc
      841 cgggcggccg accgcgaagc cgattgccgt ctccccgatc acggcgacgg cgaccgggtc
      901 cggcaacgag tccccggcgg gaaccgtcac accgaggaag gtcgccgctg tccagtcccg
      961 gcgggtgggg atccggatcg gcccggcacc gacggcgtcg gcaacccggt cgagcaaacg
     1021 aatcaggcgg gaaccgcgga gaccgggaac cgcgatcacc tcgaccctcc ccgccccctg
     1081 gtcccgggcg aactccgcct cggacatgat cagggccgag atccggtcga gaccgtcgat
     1141 tccggccgtg accttgtggc tgcgctcgac gatcctccgg gttgaaccgg aagacacttc
     1201 cgacagcgaa aagcgggtac tgcggagcga gatttcaatt gccccaagga tcaagaccgg
     1261 gccatcctag aggcaatgtg cccgaaagcc gaattaatcc tgtaaacgcc ggtattggac
     1321 ggaaagccgc tactggccgc agaccggcca ctggccgggc ccggagcgct cgtacaggag
     1381 cgcggcacgg taatcctgct cggcctccgg agcatcagac gggagaccct tgccaccgac
     1441 cgattcccag gtgccgggac tgaactggta cttgccgaag tagaggccgc tggaggaaac
     1501 gatcttcgga tcaccgcccg actcgcaatc ggcgatcgct tcgagagtct ccatggtgac
     1561 cgaccccggg aggtccccga atttttcctg ctgctgcctg gcttcgagtt tccgctgtac
     1621 ctcggcctgc tgcctttcga cgatccgttc agccgcttcg gccctggact gctggaccag
     1681 gtcaccgatc tggaaggcga cgccgaccgg cgtttccgag gtcaccgcgg caagggcgat
     1741 gtcggcaccg gaagtcccgg agccgtcctt cgaccggtcg aaggcgaggt tcccggcgat
     1801 aatcagggca gcgatcagcg ccagaatgag gattgaattt cgcgtcgtaa ggatgatttc
//
LOCUS       DAOQFI010000439         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39661_length_1856_cov_2.806774, whole genome shotgun sequence.
ACCESSION   DAOQFI010000439 DAOQFI010000000
VERSION     DAOQFI010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39661_length_1856_cov_2.806774"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..583
                     /locus_tag="PLE93_07860"
     CDS             140..583
                     /locus_tag="PLE93_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH87003.1"
                     /translation="MAIKRMDNVLIVVEDMAAVKDFFIELGMEFDGETTVEGPWVDQT
                     VGLEGVRADITMLRTPDGGCRVELSRFHNPPAVRAEPEDAPSNALGIRRIMFAVDDID
                     EVVERLVSRHEAKLVGEIVRYGDSYRLCFLRGPEGIVVGIAEELG"
     gene            648..1268
                     /locus_tag="PLE93_07865"
     CDS             648..1268
                     /locus_tag="PLE93_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNH87004.1"
                     /translation="MRRLLPLILGLALLWAPAQAGAVTAQTARFVAGFEGFVSCPYAD
                     PAGHATIGYGHLLHYGPPTRADRRKWGCLTQAQALRLLRQDLRETEIEVFDRIRGAKV
                     NAPMVTALTSFAFNLGAGYLDFVAKRGSRPATNIGRAIRVGKYRRAARQMLYFDGVIV
                     GGKRYELEGLQIRRRKEYRLMIRGIHQLRACDGACSDGPDGGLGVN"
     gene            1392..>1856
                     /locus_tag="PLE93_07870"
     CDS             1392..>1856
                     /locus_tag="PLE93_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87005.1"
                     /translation="MTHRIPHPKRILLFLTVAIAALIGFAGTASAAGQGSIVFIKNND
                     IWLANPDGSHQFRVTWDGSEDFPYLSPSQAADGTIAAGHVRDIVLMRQNGDVIREIEP
                     PALINSVSHPMDGVPVDVAISPNGKRIAWSFVGYECDGTVNCGPRTATGYTDS"
BASE COUNT          313 a          609 c          635 g          299 t
ORIGIN      
        1 gcttgagggg cttgtgtccg caaggacgtg gaggttcgac tcctctcagc cgcatgcatc
       61 cggttccggc ctgccccacg ggcgggccgg ttccgtctgc catggttcgg gtccggcatc
      121 cggcaaggag aggatcaaag tggcaataaa gcgaatggac aacgtcctca tcgtggtcga
      181 ggacatggcg gcggtaaagg acttcttcat cgaactcggc atggagttcg acggggagac
      241 gacggtcgag gggccctggg tggaccagac cgtggggctt gaaggggtcc gggcggacat
      301 caccatgctg cggaccccgg atggaggctg ccgggtggag ctgtcgagat ttcacaatcc
      361 gccggcggtc agggccgaac cggaagacgc gccgtcaaac gccctcggga tccgccggat
      421 catgtttgcg gtcgacgaca tcgacgaggt ggtcgagcgt ctggtcagcc ggcatgaagc
      481 gaagctggtc ggtgagatcg tgcgctacgg ggacagctac cggctctgtt ttctgcgggg
      541 accggaggga atagtggtcg ggatcgccga agaactcggc tgaaagcggg gttccgcgcg
      601 tctcctgtgc cgcgtcgcgg gcgcccggac tgctatccat tgaggcgatg cgtcgcctcc
      661 ttcccctcat tctgggcctg gccctgctct gggccccggc ccaggcgggc gccgtcacgg
      721 cgcagaccgc caggttcgtg gccggcttcg aagggttcgt ttcctgcccc tacgccgacc
      781 cggccggcca cgccacgatc ggctacgggc acctgctcca ttacgggccg ccgacccggg
      841 ccgatcgccg caagtggggc tgcctgaccc aggcgcaggc cctccggctg ctgcgccagg
      901 acctgagaga aaccgaaatc gaggtctttg accggatccg gggagccaag gtgaacgcgc
      961 cgatggtcac ggcgctgacc tccttcgcct tcaacctggg agcgggttac cttgacttcg
     1021 tggcgaagcg gggcagccgg ccggcgacca acatcggtcg tgcgatccgg gttggcaagt
     1081 accggcgcgc ggcgcggcag atgctctact tcgacggcgt catcgtcggc ggcaaacggt
     1141 acgagctcga gggccttcag atcaggaggc gcaaggagta ccggctgatg attcgcggca
     1201 tccaccagct gagagcctgc gacggcgcct gcagcgacgg cccggacggc ggcctgggcg
     1261 tcaactgacc gggtccggca ggcgccggct ggtcccgggc agcggcctcc cggcccggcg
     1321 caatcagtcc gcaagatccg ggcaaggtcc cctttctagg ttgctttcca cagcacccga
     1381 cgaggagacc aatgacccac cgcatccccc atcccaagcg aattcttctg ttcctgaccg
     1441 tcgcgatcgc ggccctgatc ggctttgccg gaaccgcctc ggcggccggg cagggatcga
     1501 tcgtcttcat caagaacaac gacatctggc tcgccaatcc cgacgggagc catcagttcc
     1561 gggtcacctg ggacggaagc gaggacttcc cctacctctc ccccagccag gccgccgacg
     1621 ggacgatcgc ggccgggcac gtgcgcgaca tcgtgctgat gcggcaaaac ggagacgtga
     1681 tccgggagat cgaacccccg gccctgatca actcggtcag ccatccgatg gacggcgttc
     1741 cggttgacgt ggcgatctcg ccgaacggca agcggatcgc ctggtccttc gtcggctacg
     1801 aatgcgacgg gacggtcaac tgcgggccgc gcaccgcaac cggctacacc gactcg
//
LOCUS       DAOQFI010000440         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_39966_length_1849_cov_2.986622, whole genome shotgun sequence.
ACCESSION   DAOQFI010000440 DAOQFI010000000
VERSION     DAOQFI010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39966_length_1849_cov_2.986622"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLE93_07875"
     CDS             <1..267
                     /locus_tag="PLE93_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87006.1"
                     /translation="VADQREVLTVIAGGNGIALGAIGAWFMVARSESRATGDGFEAAG
                     AIVSAAVILLLPVVVPTADFWSGLAGGIVGVLAGFTATRFGSPR"
     gene            285..641
                     /locus_tag="PLE93_07880"
     CDS             285..641
                     /locus_tag="PLE93_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87007.1"
                     /translation="MPEEQISDEQLAAAVERLSDPERFREAEQLVSRVAPGLPAVLVT
                     ALGSGGWFGESHLSETLKAATIPDEDQRLTAIRALLTEETQMGMMVGVAVGWALRQEL
                     ENGDTPGAPAIKNEES"
     gene            644..1360
                     /locus_tag="PLE93_07885"
     CDS             644..1360
                     /locus_tag="PLE93_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNH87008.1"
                     /translation="MEIRYHGHSCFELSEGDTTLIVDPFLAPNNPIADVTADDVSVTH
                     LALTHGHADHVADAVAVATKNNAKCVAMVEVANWLEMQGVENVSDPNLGGTVRYDDDG
                     LSIKLVQAWHTNTLPGSDERPFSAELGTPIGTPSGLIIEMGGLTIYDAGDPCLFGDME
                     LIGKRHGVDIALLPIGGHYTMDRDDAAYAAGLIGAQRVIPIHYDTFPPIKTDAQAFAQ
                     DIQSKGMEAIVLEPGQTASL"
     gene            complement(1508..>1849)
                     /locus_tag="PLE93_07890"
     CDS             complement(1508..>1849)
                     /locus_tag="PLE93_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87009.1"
                     /translation="DTTVPSQEADTSAVPVDHPAAETSAMPTEPEIPANPATSEGGDG
                     LEGHTTYPPDEIVNEGPATVPSEGSTDTSGQIPGTAPSTPVVTDPATPVVPTSPTPAP
                     MPSAPQTAPVE"
BASE COUNT          300 a          619 c          627 g          303 t
ORIGIN      
        1 gtggccgatc agagagaggt gctgaccgtg attgccggcg gcaacggcat cgcgctgggc
       61 gcgatcggcg cctggttcat ggtcgcccga tccgaatccc gcgccaccgg ggacggtttc
      121 gaggcggccg gagcgatcgt ctccgccgcg gtgatcctgc tgctgccggt cgtcgtgcct
      181 accgccgact tctggagcgg cctggccggc ggaatcgtcg gtgtcctggc cggtttcacc
      241 gcgacccgtt tcggctcacc ccgctgaccc tctaggctga cccggtgccc gaggagcaga
      301 tctctgacga gcaactcgcc gccgcggtag agcgactgag cgacccggaa cggtttcgcg
      361 aggccgaaca gctggtctcc cgggtcgccc cgggattgcc cgctgtactc gtcacggccc
      421 tcgggagcgg cggctggttc ggtgaatcac atctctccga gaccctgaag gcggccacga
      481 tcccggatga agaccagcgg ctgaccgcga tccgggcact gctcaccgag gaaacccaga
      541 tgggaatgat ggtcggggta gcggtcggct gggccctgag gcaggaactg gaaaacggtg
      601 acacccccgg tgcgccggca atcaagaacg aggagagctg aacatggaaa tccgctatca
      661 cggacattcg tgcttcgaac tgagcgaagg tgacacgacc ctgatcgtcg atcccttcct
      721 ggccccgaac aatccgatcg ccgacgtgac cgccgacgat gtctccgtaa cccatttggc
      781 cctgactcac ggtcacgccg accacgtggc cgatgcggtc gcggtggcga cgaagaacaa
      841 cgccaaatgc gtggcgatgg tcgaggtcgc caactggctg gagatgcagg gcgtcgagaa
      901 cgtctccgac cccaacctcg gcgggaccgt ccgctacgac gatgacggcc tctcgatcaa
      961 gctggtccag gcctggcaca ccaacaccct ccccggctcg gacgagcgtc ccttcagcgc
     1021 cgagctcggc accccgatcg gcacgccctc cggcctgatc atcgagatgg gtggcctgac
     1081 catctacgac gccggcgacc cctgcctctt cggtgacatg gagctgatcg gcaaacgcca
     1141 cggcgtggac atcgctctgc tgccgatcgg cggtcactac acgatggacc gcgacgatgc
     1201 cgcctatgcg gccgggctga tcggcgccca gcgggtgatc ccgatccact acgacacatt
     1261 ccccccgatc aagaccgatg cccaggcctt tgcccaggac atccagtcga agggaatgga
     1321 agcaatcgtc ctcgagcccg gccagaccgc ctctctctaa ctgccccaag acggagccgg
     1381 gccatccatt tctggatggc cctggcccgg cgggttgaga ttggaggccc ggcgcagacc
     1441 gggccgactg ttgatctgat ggccctggcc cggcgggttg agattggagg cccggcgggc
     1501 cgactcctta ttccaccggt gcggtctggg gagcgctcgg catcggggcc ggggtcggcg
     1561 aggtcggcac caccggggtg gccggatcgg tcaccaccgg ggtcgaaggc gcggtacccg
     1621 ggatctgccc cgaggtgtcg gtcgaacctt cgctcggcac ggtcgccggg ccttcgttca
     1681 cgatctcgtc gggcgggtag gtggtgtgcc cctcgagacc gtcgccacct tcagaggttg
     1741 cggggttggc cgggatctcc ggttcggtcg gcatggccga ggtctcggcg gccggatggt
     1801 cgaccgggac ggccgaggta tcggcttcct ggctggggac cgtggtatc
//
LOCUS       DAOQFI010000441         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40160_length_1844_cov_2.955841, whole genome shotgun sequence.
ACCESSION   DAOQFI010000441 DAOQFI010000000
VERSION     DAOQFI010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40160_length_1844_cov_2.955841"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="purH"
                     /locus_tag="PLE93_07895"
     CDS             complement(<1..980)
                     /gene="purH"
                     /locus_tag="PLE93_07895"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNH87010.1"
                     /translation="MDPTNGPVKVRRALISVSDKTGLADFAKGLADLGVEILSTGGTA
                     RALRDAGLTVIDVSEFTGQREIMDGRVKTLHPKIYAGLLARRSDPAHMEVLTEEEIPP
                     IDLVCVNLYPFEQTIAREDVGPQEAIENIDIGGPTMIRAAAKNHEDVVVLVKPECYDA
                     VIEEMNQTEGEVSASTRHWLANEAFAQTARYDAAISAWFSESYEAFPEHRVLAFEKQL
                     DLSYGENPHQRAALYSQVGLDSNVLTGMSKHHGKALSFNNVLDLDSCRKLLADLEGPA
                     CVIVKHNNPCGAAEGESALEAYEKALACDPLSAYGGVIALVLIIPWLLKKT"
     gene            complement(1034..1672)
                     /gene="purN"
                     /locus_tag="PLE93_07900"
     CDS             complement(1034..1672)
                     /gene="purN"
                     /locus_tag="PLE93_07900"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNH87011.1"
                     /translation="MTPSSPFRVVVLASGGGTNLQAVLDQLHGRGEVEVVGVGSDKPA
                     AYALERATAAKVPTAVFPRDEYEDREARDAAMADWIESTGAELVALAGYMQLVSASFV
                     ERFRGQIVNVHPALLPSFPGLDAIGQALEQGVKVTGVTVHFVDEGTDTGPVIIQRAVE
                     VPANWDREELEVAIHAIEHEIYPEAIGMIARGEVKIADDEPRKVIVTQTRGK"
BASE COUNT          297 a          610 c          594 g          343 t
ORIGIN      
        1 gtcttcttca agagccaggg aatgatcaga accagcgcga tcacgccccc gtaggccgac
       61 agtggatcgc acgccagcgc cttctcatac gcctcgagcg cgctctcccc ctcagccgcg
      121 ccgcaggggt tgttgtgctt gacgatcacg catgcgggac cttcgaggtc ggcgagcagt
      181 ttgcggcagg agtcgaggtc gagcacgttg ttgaaggaga gcgccttgcc gtggtgcttc
      241 gacatcccgg tcagcacgtt gctgtcaagg ccgacctgcg agtagagcgc ggcccgctgg
      301 tgcgggttct cgccgtagga aaggtcgagc tgcttctcga aggccagcac gcggtgctcg
      361 gggaaggctt cgtaggactc ggaaaaccag gccgagatcg cggcgtcgta acgggcggtc
      421 tgggcgaagg cttcgttggc cagccagtgg cgggtcgagg cggagacctc accttcggtc
      481 tggttcattt cctcgatcac cgcgtcgtaa cactcgggct tgaccagcac caccacgtcc
      541 tcgtggttct tggccgcggc gcggatcatg gtcgggccac cgatgtcgat gttttcaatc
      601 gcctcctgcg ggccgacgtc ctcacgcgcg atcgtctgct cgaaggggta gaggttgacg
      661 cagaccaggt cgatcggcgg gatctcctcc tcggtcagga cctccatgtg ggccgggtcc
      721 gagcggcggg cgagcagtcc ggcgtagatc ttcgggtgga gagtcttgac ccggccgtcc
      781 atgatttcgc gctggccggt gaactcggaa acgtcgatca cggtcagtcc ggcgtcgcgc
      841 agggcccggg cggtgccgcc ggtggagagg atctcgaccc cgagatcggc caaccccttg
      901 gcgaagtcgg caagcccggt cttgtccgag accgagatca gggcgcgcct gacctttacc
      961 ggtccgttgg tcggatccat gttgtccata tcaaccctct tgttcgtttc gaagcgcccg
     1021 gggacggccg gcgtcacttc ccacgtgttt gcgtgacgat caccttgcga ggttcgtcat
     1081 cggcgatctt aacttcgccc cgggcaatca tgccgatcgc ctcggggtag atctcatgct
     1141 cgatcgcgtg gatcgccact tctagttcct cgcggtccca atttgcggga acttccaccg
     1201 cccgctggat gatcaccggc ccggtgtcgg tgccttcgtc gacgaagtgg acggtcaccc
     1261 cggtcacctt cacgccctgc tcgagcgcct gcccgatcgc gtcaagtccg gggaacgagg
     1321 gaagcagagc cgggtggacg ttgacgatct gcccccggaa ccgctcgacg aaggacgccg
     1381 agaccagctg catgtatccg gccagggcaa ccagttcggc cccggtcgac tcgatccagt
     1441 cggccatcgc cgcatcccgg gcctcccggt cctcgtactc gtcccgggga aagaccgcgg
     1501 tcggcacctt cgccgcggtc gcccgttcca gcgcataggc cgccggcttg tcggaaccaa
     1561 ccccgaccac ctcgacctcg ccccgcccgt gcagctggtc aagaacagcc tgaaggttgg
     1621 tccccccacc ggaggccagc accacaacgc gaaacggaga agacggggtc acggcgccat
     1681 cttccctgat ctcgcccgat gccacccgtt aggttgaagc catgggattc acgcagggaa
     1741 cccacagaaa cacgaggcgg gcggtttggc tggccgttgc gggattgatt ctgtcccttc
     1801 tgctggtcgc cgcggtttgt ccttccccgg cggaagccat ccag
//
LOCUS       DAOQFI010000442         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40285_length_1841_cov_3.641097, whole genome shotgun sequence.
ACCESSION   DAOQFI010000442 DAOQFI010000000
VERSION     DAOQFI010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40285_length_1841_cov_3.641097"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="PLE93_07905"
     CDS             <1..1601
                     /locus_tag="PLE93_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87012.1"
                     /translation="AMLLSAGVMIETQPGEGRNDIVCFAFLIAFVAFLINGHQRRAPS
                     SGAVDEKPDPNAPLLDKGPLILAGIAAGIAISIKVSMLAPVGVIFLGMILISGKGRRV
                     TTALCLGISMFIVGGYWYVRAMLYTGGNPVPAVGWGPLHLPQPDQMPLDPRPRFSVAH
                     YITDPSIYRWWFFPRLDDAFGVLFPLILVMLAAAALWLVFKSRNKIVRVISAAALITA
                     FVYVFTPLTAAGQEFQPRGFFTNTRYLLPGLLLAAVMLPLIRQLREPQARAKKTLIFL
                     TVVYAITVLTSPKWYPSFLPGAIFLTLAIVWAPVGLSWMKDNTRVPKYVIVGCVAAIA
                     IIGAIWGRGEEVGYADKHYTRTTLFLQEGGPQDAFEFTRNLKDKRIALAGSGEIFFGQ
                     YGFYGVDRSNHVQYIGQEGDNGTYRLITNCREFINTINEGDYDYLITSEYTQDAPEAD
                     YWFPVRAWVDGDPNAELVVSEDDITPQPDYVYKINGKLDPDRCAKLDQAAIRQVRTDA
                     LQAIKDREAEAAAAEAAAESSDSQ"
     gene            1650..1723
                     /locus_tag="PLE93_07910"
     tRNA            1650..1723
                     /locus_tag="PLE93_07910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1684..1686,aa:Arg,seq:tct)
BASE COUNT          309 a          622 c          577 g          333 t
ORIGIN      
        1 gcgcgatgct cctttcggcc ggggtgatga tcgagaccca gcccggcgag ggtcgaaacg
       61 acatcgtctg cttcgccttc ctgatcgcct tcgtcgcctt cctgatcaac gggcaccaaa
      121 ggcgggcgcc cagttccgga gcggtggacg aaaagcccga cccgaacgct cctctgcttg
      181 acaagggccc gctgatcctc gccggtattg cggccggcat cgcgatctcg atcaaggttt
      241 cgatgctggc gccggtcggc gtgatcttcc tcggcatgat cctgatcagc ggcaagggac
      301 gccgggtcac gaccgcgctc tgcctcggca tctcgatgtt catcgtcggc ggctactggt
      361 acgtgcgggc gatgctctac accggcggca acccggtgcc ggccgtcggt tggggcccgc
      421 tccacctgcc gcagccggac cagatgccgc tcgacccgcg cccgcggttc tcggtcgcgc
      481 actacatcac cgacccgagc atctaccgct ggtggttctt tccccggctc gatgacgcct
      541 tcggggtgct gttcccgctg atcctggtca tgctcgcagc ggccgcgctc tggctggtct
      601 tcaagtcccg caacaagatc gtccgggtga tctccgcggc cgccctgatc accgccttcg
      661 tctatgtgtt caccccgctg accgcggccg gccaggagtt ccagccgcgc ggtttcttca
      721 ccaacaccag gtacctgttg ccggggctcc ttctggccgc ggtgatgctg ccgctgatcc
      781 gccagttgcg tgagccgcag gcgagggcga agaagaccct gatcttcctc accgtggtct
      841 acgcgatcac ggtgctgacc tcgcccaagt ggtacccgag cttcctgccc ggcgccattt
      901 tcctcaccct ggcgatcgtc tgggccccgg tcggcctgag ctggatgaag gacaacacca
      961 gggtgccgaa gtacgtgatc gtgggctgcg tcgccgcgat cgcgatcatc ggcgcgatct
     1021 ggggccgtgg tgaagaggtc ggttacgccg acaagcacta cacccggacc actctcttcc
     1081 tgcaggaagg cggcccccag gacgccttcg aattcacccg gaacctcaag gacaagcgga
     1141 tcgcgctcgc cggttcgggt gagatcttct tcggccagta cggcttctac ggggtcgatc
     1201 gctcgaacca cgtccagtac atcggtcagg agggtgacaa cggcacctac cgtttgatca
     1261 ccaactgtcg tgaattcatc aacacgatca acgaaggtga ctacgactac ctgatcacca
     1321 gcgagtacac ccaggacgcg ccggaagccg attactggtt cccggtccgg gcctgggttg
     1381 acggtgaccc caacgcggag ctcgtcgtct cggaagacga catcactccg cagccggatt
     1441 acgtctacaa gatcaacggc aagctcgatc cagaccgctg cgccaagctc gaccaggcag
     1501 cgatccgcca ggtgcggacc gacgcgctcc aggcgatcaa ggatcgtgag gcagaagccg
     1561 cggccgcgga agccgccgca gagtcatccg attcgcagta gccgcttccg cccggccagc
     1621 ggcgggaatg gctagcgtta ccttttcgcg cccccgtagc tcagctggat agagcgccgg
     1681 ccttctaagc cggatgtcgc aggttcgaat cctgccgggg gtgccttcgt cccgctgtga
     1741 agaaggcgat gatcgtgacc accaggatca ggctgccgta gacgagggcg gtggcgtgga
     1801 ggccgtaccg gtccgaggcg aagccgccga tcagggcggg g
//
LOCUS       DAOQFI010000443         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40353_length_1840_cov_2.250420, whole genome shotgun sequence.
ACCESSION   DAOQFI010000443 DAOQFI010000000
VERSION     DAOQFI010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40353_length_1840_cov_2.250420"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLE93_07915"
     CDS             complement(<1..342)
                     /locus_tag="PLE93_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87013.1"
                     /translation="MENSNTTDAVAITLDRDHENGPDWTAISQAANEQDQHPDGPRIV
                     DRVRELWENPERFTHVSKVMVRDGHTLVVGGGNTATEINDRETVGKFKELGIHEKAGF
                     DGRWARGTWDSQ"
     gene            complement(439..>1840)
                     /gene="dndC"
                     /locus_tag="PLE93_07920"
     CDS             complement(439..>1840)
                     /gene="dndC"
                     /locus_tag="PLE93_07920"
                     /inference="COORDINATES: protein motif:HMM:NF005316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HNH87014.1"
                     /translation="CLQLVWSAVSELPKAKRTNPVHVITTDTMVENPVVAAYVEGQLG
                     HMNQAATDQAMPIEAHRLTPDVEDRFFVNLIGRGFAAPRPKFRWCTERLKIKPSNRFI
                     RDVISQAGEAILVLGTRKAESAARAKSMEHHEKGRVRDRLSPNGSLPGSLVYSPIEAF
                     TDDDVWTFLMQHSNTPWGTRNHDLLTLYQGATEDGECPLVVDSSTPSCGDSRFGCWTC
                     TMVEQDRSMGAMIANDEQKEWMLPLLAIRDELDVRDSEGNRNDYHLRDFRRSNGNLQL
                     FHNEEPAPGKTQRETLVPGPYLQEHREYLLRRVLEAQQTVRKIGPPEVAAIELLTIED
                     LEAIRAHWLTRFHEFEDRLPVIYEQVTGNRYPAETVKDDGAALGPEYVEILRDICGDD
                     TEQFRMIREMLSIEQQERSKLRRHGILDKLERAVRKGGFENEQDALENALHRRRELAT
                     GRPAEGEQQVLDSTLA"
BASE COUNT          266 a          551 c          558 g          465 t
ORIGIN      
        1 ctggctgtcc caggtgccgc gggcccatcg tccgtcgaag ccggcctttt cgtggatgcc
       61 tagctccttg aacttcccga cggtttcccg gtcgttgatt tcggtcgcgg tgttgcctcc
      121 acctacgaca agggtgtggc cgtcacggac catcactttc gaaacatggg tgaaccgttc
      181 ggggttctcc caaagctccc tgacccggtc gacgatccgc gggccgtcag gatgctggtc
      241 ttgctcgttg gcggcctggc tgatcgctgt ccagtcaggg ccgttttcgt ggtcacggtc
      301 gagagtgatc gcgaccgcat cggttgtgtt gctgttttcc attggtgtct ccttgcttgt
      361 tttcgggaag cgggcagcgc accggtccgt cactggtccg gtggctgcct tcccccctgt
      421 tgttgatggt gttggcggtt aggccagggt gctgtcaagc acctgttgct caccttcggc
      481 cggtcggccg gtggcgagtt ctctgcgtcg gtgaagcgcg ttctcgaggg cgtcctgctc
      541 gttttcgaag ccgcccttcc tcaccgctct ttcgagcttg tcgagaatgc cgtgacggcg
      601 aagtttcgac cgttcctgct gttcgatcga aagcatctcc cgaatcatcc ggaactgctc
      661 ggtgtcatcc ccgcagatat cccggaggat ctccacatat tccgggccga gcgccgcccc
      721 gtcatccttg accgtttcgg ccgggtatct gttcccggtt acctgctcgt agatcactgg
      781 cagccggtcc tcgaattcgt gaaaccgggt cagccagtgc gcccggatcg cttcgaggtc
      841 ctcgatcgtt agaagctcga tcgctgccac ttcgggcggt ccgatcttcc tgactgtctg
      901 ctgcgcttcg agtacccgcc ggagcagata ttcgcgatgt tcttgcaggt acggtcccgg
      961 caccagggtt tccctttggg ttttcccggg ggccggttct tcgttgtgga agagctgcag
     1021 gtttccgttt gaccggcgga agtcccgcag gtggtagtcg ttgcggtttc cctcgctgtc
     1081 tcttacatcg agttcgtcac ggatcgccag aagcggcaac atccattcct tttgttcgtc
     1141 gttggcgatc atcgctccca ttgaccgatc ctgctcaacc atggtgcagg tccagcaacc
     1201 gaaccttgaa tccccgcagg acggggtgct tgagtcaact accagcgggc attcgccgtc
     1261 ctcggttgct ccctggtaca gggtgagcag atcatggttg cgggtacccc atggtgtgtt
     1321 gctgtgctgc atcaggaagg tccagacgtc atcgtcggtg aacgcttcga tcggtgaata
     1381 gaccaatgat ccgggtagtg atccgttcgg ggatagacga tccctgaccc ggcctttttc
     1441 atggtgttcc atgcttttgg cccgcgcagc tgattccgct ttgcgggttc cgagcacaag
     1501 gatcgcttca ccggcctggc tgatcacgtc acggatgaac cggttggacg gtttgatttt
     1561 caatcgttcg gtacaccacc ggaacttcgg tcttggtgcc gcgaagccgc ggccgatcag
     1621 gttgacgaaa aaccggtctt ccacgtccgg ggtgagccgg tgcgcttcga tcggcatggc
     1681 ctggtcggtg gcggcctggt tcatgtgacc gagctgccct tcgacgtagg cggcaacgac
     1741 cgggttttcg accatggtgt cggtcgtgat cacatgtacc gggttagtcc ttttcgcctt
     1801 cggcagttca ctgaccgcgc tccagaccag ttgaaggcag
//
LOCUS       DAOQFI010000444         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40372_length_1839_cov_3.637332, whole genome shotgun sequence.
ACCESSION   DAOQFI010000444 DAOQFI010000000
VERSION     DAOQFI010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40372_length_1839_cov_3.637332"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLE93_07925"
     CDS             complement(<1..430)
                     /locus_tag="PLE93_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87015.1"
                     /translation="MSSDDRTRVMRNDEPPRRRHDEDPHYTPEEQKKHLRNLIIVLIA
                     VIVGLVIAVIVISSGGDSSDDTSATTGDTAVTTPVVPVTPSGETGTTDSSGEDDGGVT
                     PTDTGTDTSGGISPDTSTGTGTDTGGVTPDTSTGTDTGGVT"
     gene            complement(444..1640)
                     /locus_tag="PLE93_07930"
     CDS             complement(444..1640)
                     /locus_tag="PLE93_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HNH87016.1"
                     /translation="MNEQVRADEASFVMQTYKRAPVEFVRGEGALLFDREGRQYIDFL
                     GGISVASVGHCHPAVVEAIRQQAGELLHVSNLFYTAPMVELAKRLSESSLGGKVFFCN
                     SGTEANECAIKIARKHARSRGVESPEIVSFESDFHGRSYGALSATPGLAANPDFAPML
                     PGFRSVPFDDAAALRDAVGPDTAAVLIEPIQGEAGVFPLSDNTLLAAREACDEAGALL
                     IFDEVQTGIGRTGSLWAYEQGPVRPDVLTTAKALGGGTAIGACIGNPETGEVLAAGDH
                     GSTFAAGPVASSAALAVLDLVDDPEMLRRIRALGASLTERLNALDGVAEVRGRGLMIG
                     VRLEPGLNAGEVNAALLGNGLVANAPRPDTLRFLPPFVLTDEQADRAVALIDETLAGY
                     HPDESN"
     gene            complement(1637..>1839)
                     /locus_tag="PLE93_07935"
     CDS             complement(1637..>1839)
                     /locus_tag="PLE93_07935"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935081.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNH87017.1"
                     /translation="RISETTVAEVEDALESISGGMRPKLTACVDAIRGGAQNAYIIDG
                     RRPHSLLLELFTDAGIGTMVTP"
BASE COUNT          277 a          602 c          644 g          316 t
ORIGIN      
        1 gggtgactcc cccggtgtcg gtgccggtgc tggtatcggg agtgacaccg ccggtatccg
       61 tgccggtacc ggtgctggtg tcgggcgaga tgccgccgga agtgtccgtg ccggtgtcgg
      121 tcggggtgac cccgccgtca tcttcaccgg aggaatcggt ggtgccggtt tctccactcg
      181 gggtgaccgg gacgaccggt gtggtcacgg cagtgtctcc ggtggttgcc gaagtgtcgt
      241 cgctgctgtc gccgccggaa ctgatcacga tcaccgcaat caccaggccc acgatcacgg
      301 cgatcagcac gatgatcagg tttcgcaggt gcttcttctg ctcttcgggc gtgtagtgag
      361 ggtcctcgtc gtggcgccgg cggggcggct cgtcgtttct catcactctt gtgcggtcat
      421 cgctactcat cggaaatctc cagtcaattc gattcgtccg gatggtaccc ggccagcgtc
      481 tcgtctataa gggcgacagc ccggtcagcc tgctcatcgg taaggacaaa cggcggcaga
      541 aagcgaaggg tgtcgggtcg cggcgcgttg gccaccagcc cgttcccgag cagggccgca
      601 ttgacctcgc cggcattcaa accgggttcc agccggaccc cgatcatcaa gcccctcccc
      661 cgcacttccg ccaccccgtc aagggcgttc agcctttccg tcagggaggc gccgagcgcg
      721 cggatccgcc gcagcatctc ggggtcgtcg accaggtcga ggacggcgag cgcggcgctg
      781 gaagcgaccg gtccggccgc gaaggtcgag ccgtgatcgc cggcagccag cacctccccg
      841 gtctcggggt tgccgatgca ggcgccgatc gccgtgcccc cgccgagcgc cttggccgtg
      901 gtcaggacat ccggtcgcac cggcccctgc tcgtaggccc agagcgagcc ggtccggccg
      961 atcccggtct ggacctcgtc gaagatcagc agcgccccgg cctcgtcgca ggcttcgcgg
     1021 gcggcgagca gggtgttgtc ggaaagcggg aagacgccgg cttcgccctg gatcggttcg
     1081 atcagcaccg cggcggtgtc cggaccaacg gcgtcacgaa gcgccgcggc atcgtcgaac
     1141 ggaaccgagc ggaagcccgg cagcatcggc gcgaagtccg ggttggcggc caggcccggc
     1201 gtggctgaaa gggcaccgta actgcggcca tggaagtcgc tttcaaacga gacgatttcc
     1261 gggctttcga ccccgcggct ccgggcgtgc ttccgggcga tcttgatcgc gcattcgttg
     1321 gcttcggtgc cggagttgca gaagaagacc ttgccgccca gcgagctctc cgagagccgt
     1381 ttggccagct cgaccatcgg ggcggtgtag aagaggttcg agacgtgaag gagttctccg
     1441 gcctgctgcc ggatcgcctc cacgaccgcc gggtggcagt ggccgaccga ggcgaccgag
     1501 atgcccccga ggaagtcgat gtactgacgg ccttcccggt cgaagagcag cgcgccttcg
     1561 ccccggacga actcgaccgg ggcccgtttg taggtctgca ttacaaaact ggcctcgtcg
     1621 gcccggacct gctcgttcac ggggtcacca tggtgccgat gccggcgtcg gtgaagagtt
     1681 cgagcagcag cgaatgggga cggcggccgt cgatgatgta ggcgttctgg gcgccgccgc
     1741 ggatcgcgtc cacgcaggcg gtcagcttgg gtcgcatgcc gccggagatc gactccaggg
     1801 catcctcgac ttcggccacg gtggtctcgg agatccgcg
//
LOCUS       DAOQFI010000445         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40523_length_1836_cov_3.048287, whole genome shotgun sequence.
ACCESSION   DAOQFI010000445 DAOQFI010000000
VERSION     DAOQFI010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40523_length_1836_cov_3.048287"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..985)
                     /locus_tag="PLE93_07940"
     CDS             complement(50..985)
                     /locus_tag="PLE93_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH87018.1"
                     /translation="MERERPARPHSDQRPPIACLGEAIADLICERNLAKGEAPGPFVP
                     WPGGALANVAVAIARSGAPAALVGGVGGDGWGRWLADGLESEGVSTRWLATLDGADTP
                     VALIEFDSGNEPAFQVYGEHIGPTMAATGDFLEEAITGSRAVVIGSNTMVGEVERNVT
                     RRAVELAAAAGLPVLLDPNYRPNRWTSEEPARDFCRELSGSATVVKLNQGEAEMITGI
                     GEPLAAARDLLKLGPELVVVTNGEGEIVTAGAVEARHRPEPVETVSPLGAGDAFMGGL
                     AAGLAGLDWDLSRAAEVLPRAALAAAAVCGHWGAQ"
     gene            1055..>1836
                     /locus_tag="PLE93_07945"
     CDS             1055..>1836
                     /locus_tag="PLE93_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNH87019.1"
                     /translation="MPALIVRIGEWLPRGWPDLFRQLLLYVLADTFYEMARGMADGRA
                     NVAFANGERVIDTERTLGMFFEPGVQSALHGFTWLIDIANTIYLNSQFTVALGFLIWM
                     YLFRNDYYYFFRNMLFVSMGIALVGYIGFPTAPPRMYPEYGFIDTVNAQSSVNHDSAL
                     AKLFINPFAAVPSMHCAVALMIGVTGFQVCRNWFAKTFWAGWPLVVAWVTVVTANHYW
                     IDAVLGWGVAGLSFLIASRVLAEIKPETWAWSREAERQRAEA"
BASE COUNT          277 a          665 c          569 g          325 t
ORIGIN      
        1 gccgtttcca ttccttcttc gcgactttcg cccgccaggc agccaccggt tactgggctc
       61 cccagtgccc gcagaccgcg gccgccgcga gggccgcccg ggggagcacc tcggcggccc
      121 gggaaaggtc ccagtcgagc ccggcgagac cggccgcaag gccgcccatg aaagcgtcac
      181 ccgctccgag cggacttacc gtttcgaccg gctcgggacg atgccgcgcc tcgaccgcac
      241 cggcggtgac gatttccccc tcaccgttgg tgaccacgac cagctccggg ccgagcttga
      301 ggagatcccg ggccgcggcg agcggctccc cgatcccggt gatcatttcc gcttccccct
      361 ggttcagctt gaccaccgtt gccgacccgc tcaattcccg gcagaaatcg cgggccggtt
      421 cttcgctggt ccagcggttg ggccggtagt tcgggtcgag cagcaccggc agcccggccg
      481 cggcagccag ctcgaccgcg cgccgggtca cattccgctc gacttcgccg accatcgtgt
      541 tcgaaccgat gaccaccgcc cgggaaccgg tgatcgcctc ttcaaggaag tcgccggtgg
      601 cggccatggt cggcccgatg tgttcgccgt agacctggaa agcgggctcg tttccggaat
      661 cgaactcgat cagggcgacc ggagtgtctg ccccgtcgag tgtggccagc catctggttg
      721 agacgccttc ggattccagc ccgtcggcga gccagcgccc ccagccgtca ccgccaaccc
      781 cgccgaccag ggcggccggg gcgccgcttc gggcgatcgc cacggctacg ttagccagcg
      841 cccctcccgg ccacggaacg aagggcccgg gtgcttcccc cttggccagg ttccgctcgc
      901 agatcagatc cgcgatcgcc tcgccgagac aggcgatcgg gggtcgctga tcactgtggg
      961 gtcgggctgg ccgttctcgc tccatgactg gaaagccgga gcctacccga ggcttcctga
     1021 gagaaatctg cgcgcgccat tagactgagc agtcatgccc gcgctgatcg tccggatcgg
     1081 ggagtggctc ccccgcggct ggcctgacct ctttcgccag ctcctgctct atgtgctcgc
     1141 cgacaccttc tacgagatgg cgcgcggaat ggccgatggc cgggccaatg tcgccttcgc
     1201 caacggcgaa cgggtgatcg acaccgaacg caccctcggg atgttcttcg aacctggtgt
     1261 gcagagcgcc cttcacggct tcacctggct gatcgacatc gccaacacga tctacctcaa
     1321 cagccagttc acggtggcgc tcggcttcct gatctggatg tacctgttcc gcaacgacta
     1381 ctactacttc ttccgcaaca tgctcttcgt ctcgatgggc atcgcactgg tcggctacat
     1441 cggcttcccg accgccccgc cgcggatgta ccccgagtac ggattcattg acacggtcaa
     1501 cgcccagtcc agcgtcaacc acgactccgc cctggccaag ctcttcatca accccttcgc
     1561 cgcggtgcct tcgatgcatt gcgcggtcgc cctgatgatc ggcgtcaccg gtttccaggt
     1621 ctgcaggaac tggttcgcca agaccttctg ggcgggctgg ccgctggtgg tcgcctgggt
     1681 caccgtggtc accgcgaacc actactggat cgatgccgtg ctcggctggg gcgtcgccgg
     1741 gctctccttc ctgatcgctt cgagagtgct ggccgagatc aaacccgaga cctgggcctg
     1801 gagccgcgag gcggagcgcc aacgggccga ggcctg
//
LOCUS       DAOQFI010000446         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40562_length_1835_cov_3.292135, whole genome shotgun sequence.
ACCESSION   DAOQFI010000446 DAOQFI010000000
VERSION     DAOQFI010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40562_length_1835_cov_3.292135"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..661
                     /locus_tag="PLE93_07950"
     CDS             80..661
                     /locus_tag="PLE93_07950"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HNH87020.1"
                     /translation="MAEVIVPAREARTMEVKKGQTLEIVDLEGQQVGDFTAWLASDPN
                     EYLSPSHTVSSLSKLVPEVGDSILSNHRRPLLKILRDDVGRHDLVVPCCDPERYAIDY
                     GEPDHGSCLLSLETAIEGTNLPLRGEMCWNVFMNNQLEDGKIVTYEPPHPAGSTIAME
                     VLEDMTVALSACPQDISAVNAFNPTPMAMRVSD"
     gene            691..1323
                     /locus_tag="PLE93_07955"
     CDS             691..1323
                     /locus_tag="PLE93_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNH87021.1"
                     /translation="MAEAARIITGAGLVEAFGHVSARAGDGFLITATRPLGGAGERDV
                     YRVDADGEAPEGATDLPLETPMHAAVYAARPEVQAICRTHSPAAVRAGARQVVPPLVH
                     GLGGLAGEVALCGRTDLVTDREAGGEISRDLGSADCLLIRGNGSIATGRSLAEAVVRA
                     RYLEERCLAGAGMGQEEGWRADLVDPRARWFEKETTRAWAWMRWRYGGEK"
     gene            1394..>1835
                     /locus_tag="PLE93_07960"
     CDS             1394..>1835
                     /locus_tag="PLE93_07960"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH87022.1"
                     /translation="MSFTTSWKKIMLLLAATMALAVGVAACGSSDDDSGDSGSSTAGG
                     TQDATLVLDFVPGAVHSGIYEAVQKGYYEDNGINLKIIEPTSTADTLKLIDAGKADFG
                     IADGIDLATQITDGREAQGIMALLQRPPGGLITLEDEGITDPKQL"
BASE COUNT          331 a          596 c          631 g          277 t
ORIGIN      
        1 gtgatcgacg gggaagtgcg tgcgctcagg tcccggaaca ccccggctcc cgacggggcc
       61 tgtgaaatca aggagaagaa tggctgaagt gatcgtcccg gcccgcgagg cacggaccat
      121 ggaagtgaag aaagggcaga ccctggagat cgtcgatctc gaaggccagc aggtcggtga
      181 cttcaccgcc tggctcgcgt ccgacccgaa cgagtacctg tcgccttccc acacggtctc
      241 ctcgctctcg aagctggtgc cggaagtcgg ggactcgatc ctctccaacc atcgccggcc
      301 gctgctgaag atccttcgcg atgacgtcgg ccgccacgac ctcgtcgttc cgtgctgcga
      361 tcccgagcgc tacgcgatcg actacggcga gcccgaccac ggctcctgcc tgctttcgct
      421 ggagaccgcg atcgagggca cgaatctccc gctccgcggc gagatgtgct ggaacgtctt
      481 catgaacaac cagctcgagg acggcaagat cgtcacttac gagccgcccc atccggccgg
      541 atcgacgatc gcgatggagg tgctggaaga catgaccgtc gccctttcgg cctgccccca
      601 ggacatctcc gcggtaaatg ccttcaaccc gaccccgatg gcgatgaggg tgagcgactg
      661 actggatcga ctcatacgag cgcggccgag gtggccgaag cggcccggat catcaccggg
      721 gcaggcctgg tcgaagcctt cggccacgtt tcggcgcgcg cgggtgacgg cttcctgatc
      781 accgctaccc gcccgctcgg cggagccggc gagcgggacg tgtaccgggt cgacgccgac
      841 ggcgaagcgc ccgagggggc gaccgacttg ccgctcgaga cgccgatgca cgcggcggtc
      901 tatgcggccc ggcccgaggt ccaggcgatc tgccggaccc attcgccggc cgcggtgcgg
      961 gccggcgccc ggcaggtcgt gccgcccctg gtgcatggtc tcggcgggct ggccggtgaa
     1021 gtcgcgctct gcggccgcac cgacctggtc accgatcggg aggccggggg cgagatctca
     1081 agagatcttg gcagcgcgga ttgcctgctg atccgcggga acggttcgat cgcgaccggg
     1141 cgcagcctgg ccgaagcggt ggtccgggcc cgctatctgg aggaaaggtg tctggccgga
     1201 gcgggtatgg gccaggagga aggttggagg gccgacctcg tggatccccg tgccagatgg
     1261 ttcgaaaagg aaacgacccg ggcctgggcc tggatgcgat ggaggtacgg cggagaaaaa
     1321 tagggatcgc gttgaaccgg tcccgggtga gaaccggggc gggtgtttcg aactacaacc
     1381 agagagagga ctcatgagtt tcacaaccag ttggaagaag atcatgttgc tgctggccgc
     1441 aacgatggcg ctcgccgtcg gggtcgccgc ctgtggctcg agcgacgacg actcgggtga
     1501 ctccggctcc agcacggccg gtggcaccca ggacgcaacc ctggtgctgg acttcgtgcc
     1561 cggcgccgtt cactccggca tctacgaggc cgtccagaag ggctactacg aggacaacgg
     1621 catcaacctc aagatcatcg agccgacctc gaccgccgac acgctgaagc tgatcgacgc
     1681 cggcaaggcc gacttcggca ttgccgacgg catcgacctg gcgacccaga tcaccgacgg
     1741 tcgcgaggcc cagggaatca tggcgctgct ccagcgtccg cccggaggcc tgatcaccct
     1801 ggaggatgaa ggcatcaccg acccgaagca gcttg
//
LOCUS       DAOQFI010000447         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40636_length_1833_cov_3.377953, whole genome shotgun sequence.
ACCESSION   DAOQFI010000447 DAOQFI010000000
VERSION     DAOQFI010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40636_length_1833_cov_3.377953"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..612)
                     /locus_tag="PLE93_07965"
     CDS             complement(61..612)
                     /locus_tag="PLE93_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH87023.1"
                     /translation="MPEQSRKPRVSQEARRAEVIDAALIEFGRCGYAGTSTSMIANRA
                     GIRQPYIYALFENKRALFLACHEVLNDRIRETFRNAALPEDSPYERVRKMGLAYLGLL
                     HDDDRVRCHLQVFAAAGSDDLKEPIREGFNRLFEDVLEMSEASRPEVARFFATGMLLN
                     AMAALDEPFEMIRYLEVTPEDEL"
     gene            complement(726..>1833)
                     /locus_tag="PLE93_07970"
     CDS             complement(726..>1833)
                     /locus_tag="PLE93_07970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNH87024.1"
                     /translation="QNKATMDRLREYPHWLATRNLSNEASDESVQALVQAVKGRYDLA
                     RSWYRTKAKLLEVDRLADYDRMAAIATDDVTIEWEEGRQIVQSAFDSLAPQAGEVVGR
                     FFEDRWIDAPPAPSKRGGAFSASTVPSVHPYVMLNYTDRRRDVLTLAHELGHGLHQAL
                     AAKQGIFHQDTPLTVAETASVFAEELVFGRLLAAEKDPASRLGLLSESVEGQIATIFR
                     QIAMNQFEDAVHTARREEGELSVERFGELWAATQEDLLGDSVEVTDGYRSWWSYVPHF
                     IGSPGYVYAYAYGQLLALSVYRLYEEQGEAIVPGYLEMLTAGGSRSPEQLGALVGVDL
                     SDPSFWDNGLDLVAGQIDAAQDAAEEVLRARSQG"
BASE COUNT          316 a          585 c          600 g          332 t
ORIGIN      
        1 tcccggtggc cagcagaagg ccggtcacca gcagggccgt gagtctgggg tgagggccgg
       61 tcaaagttcg tcctccgggg tgacttccag atagcggatc atctcgaacg gttcgtccag
      121 ggcagccatc gcgttgagca gcatgccggt cgcgaagaag cgggccactt cgggccggct
      181 cgcctcgctc atctcgagca cgtcttcaaa gagccggttg aacccttccc ggatcggttc
      241 cttcaggtcg tcgctgccgg ccgcggcaaa gacctggagg tggcagcgga cccggtcgtc
      301 atcgtgaagc aggccgagat aggcgagtcc catcttccgg actcgttcgt agggactgtc
      361 ctcgggcagg gccgcgttcc ggaaagtttc ccggatccgg tcgttgagaa cttcatgaca
      421 ggcgagaaag agggcccgct tgttctcgaa cagggcgtag atatacggct ggcggattcc
      481 ggcccggttg gcaatcatcg aggttgaagt gccggcgtag ccgcagcgcc cgaactcgat
      541 cagggccgcg tcgatgacct cggctcgccg agcttcctgt gaaaccctcg gttttctcga
      601 ttgctctggc atccctggcc cttcggtcaa ccgggtaacc gtcctttacc cggatccaat
      661 gttttcgcac ccttcggccg gtccgccgga aagagcgccg gtgcaaaaga cgggtcagat
      721 gtcgatcaac cctggcttcg ggcccgaagc acttcttctg ctgcgtcctg cgctgcgtcg
      781 atctgtcctg ccaccagatc caatccgttg tcccagaacg aggggtcact gagatcaacc
      841 ccgaccagcg ccccgagttg ctctggggaa cgggagccgc cggcggtcag catctccaga
      901 taaccgggca cgatcgcttc gccctgttcc tcgtagaggc ggtagaccga gagcgcgagc
      961 agctggccgt aggcgtaggc gtagacgtaa ccgggggacc cgatgaagtg gggcacatac
     1021 gaccaccagg agcggtagcc gtcggtcact tcgacggaat ctccgagcag atcctcctgg
     1081 gtcgcggccc agagctcgcc gaaccgctca accgaaagct cgccttcctc gcggcgggcg
     1141 gtgtggaccg cgtcctcgaa ctggttcatg gcgatctgcc ggaagatcgt cgcgatctgg
     1201 ccttcgaccg attcggaaag caggccgagc cgggaagccg gatccttctc ggccgccagc
     1261 aaccggccga agaccagttc ctcggcgaag accgaagcag tttcggccac ggtcagcggc
     1321 gtgtcctggt ggaagatgcc ctgctttgcc gccagcgcct ggtgaaggcc gtggccgagc
     1381 tcgtgggcca gcgtgagcac gtcccggcgc cggtcggtgt agttcagcat cacgtaggga
     1441 tgaaccgaag gcaccgtcga agccgagaag gcgccgcctc gcttgctcgg ggccggcggc
     1501 gcgtcaatcc agcggtcctc gaagaagcgt cccacgacct caccggcctg cggggcaagc
     1561 gaatcgaagg cgctctggac gatctggcgg ccttcctccc actcgatcgt cacgtcatcg
     1621 gtggcgatcg ccgccatgcg gtcgtagtcg gcgagacgat cgacttccag cagtttcgcc
     1681 ttggtccggt accaggaacg ggcgaggtcg tagcggccct tcaccgcctg gaccagggcc
     1741 tggaccgatt cgtcggaagc ttcgttgctg aggttgcgtg tggccagcca atgcggatac
     1801 tcacgcagcc ggtccatggt cgccttgttc tga
//
LOCUS       DAOQFI010000448         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40681_length_1832_cov_3.707935, whole genome shotgun sequence.
ACCESSION   DAOQFI010000448 DAOQFI010000000
VERSION     DAOQFI010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40681_length_1832_cov_3.707935"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /locus_tag="PLE93_07975"
     CDS             complement(<1..1688)
                     /locus_tag="PLE93_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNH87025.1"
                     /translation="MSSTYTPTNRSRTLADLLPNSAARHGSDGAFLFKNEAGSWEETS
                     YEKVAAEVQALTLGLKALGVQRGDKVAIIGNTRPEWTLFDFAAMSTGATVVPIYQSNS
                     PSECQYVLEHSESRVVIAENEDQLAKIREVRDSLPALENVVIMIGEADDAISMNELRA
                     KGDSGSAEDYKASYEAVEPDDLCKIVYTSGTTGPPKGCMISHGNYRAMLDMADSASIV
                     EEGETSYLFLPLAHVFALLIQYGMIDIGGRIAFWERDQLKIVPNLAEVKPESFPSVPR
                     IFEKVHDTAVATAEGEGGLKAAIFRWAFRVGKRYRKAEAQGKKPGFLLTKQFNFADKA
                     VFSKIREIFGGNLRVAISGAAPIDPEILRFYEAAGVPLFEAWGMTETSTGGTTNMPGE
                     NRIGTVGKPVDGVELRIAEDGELLIKGDNVFQGYYKNPEATAETITDGWLHTGDIASI
                     DEDGYVSIIGRKKEIIITAGGKNITPVNIEQEVKRHPLVSQCVVIGDRRPYLIALITL
                     DQEALTRFAAEQGMLDEPGAMVGSPLVRETLEQHIEEVNRNFARVEQIKRFHILP"
BASE COUNT          293 a          562 c          601 g          376 t
ORIGIN      
        1 ggcaggatgt ggaagcgctt gatctgctcg acccgggcga agttcctgtt gacttcctcg
       61 atgtgctgct cgagcgtctc gcggaccagt gggctgccga ccatcgcacc cggctcgtcc
      121 agcatgccct gctcggcggc gaagcgggtc agcgcttcct ggtcgagggt gatcagggcg
      181 atcaggtacg gccgccggtc accgatcacc acgcactggg aaacgagcgg atgccgcttg
      241 acttcctgct cgatgttgac cggggtgatg ttcttgccgc cggcggtgat gatgatctcc
      301 ttcttgcggc cgatgatcga gacgtagccg tcctcgtcga ttgaggcgat gtcaccggtg
      361 tggagccagc cgtcggtgat cgtctcggcc gtcgcctcgg ggttcttgta atacccctgg
      421 aagacgttgt cgcccttgat cagcagctcg ccatcctcgg cgatcctgag ttcgaccccg
      481 tcgaccggct tgccgaccgt gccgatccgg ttttcgcccg gcatgttggt ggtgccaccg
      541 gtcgaggtct cggtcatgcc ccaggcctcg aacaagggca ctccggccgc ttcgtagaag
      601 cggaggatct ccggatcgat cggcgcggcg ccgctgatcg cgacccgcag gttgccgccg
      661 aagatctccc gaatcttgga gaagaccgcc ttgtcggcga aattgaactg cttggtcagc
      721 aggaagccgg gctttttgcc ctgggcctcg gccttgcggt aacgcttgcc gacccggaaa
      781 gcccagcgaa agatggccgc cttgaggcca ccctcgccct cggccgtggc gaccgcggtg
      841 tcgtggacct tctcgaaaat gcgggggacc gagggaaagg actcgggctt gacctcggcc
      901 aggttgggga cgattttcag ctggtcacgc tcccagaagg cgatccggcc gccgatgtcg
      961 atcatcccgt actggatcag cagggcgaag acatgcgcga gcggcaggaa cagatagctg
     1021 gtctcgcctt cctcgacgat gcttgccgag tcagccatgt ccagcatcgc ccggtaattg
     1081 ccatgggaga tcatgcagcc cttgggcggc ccggtcgtgc cggaggtgta gacaatcttg
     1141 cagaggtcgt cgggttcgac cgcctcgtag ctcgccttgt agtcctcggc gctgccggaa
     1201 tcacccttcg cccgcagctc gttcatgctg atcgcgtcgt cggcttcgcc gatcatgatc
     1261 acgacgtttt caagggccgg cagtgaatcc ctgacctcgc ggatcttggc cagttgatcc
     1321 tcgttttcgg cgatcacgac ccgggactct gaatgctcga gcacgtactg gcactcggag
     1381 ggggagttgc tctggtagat cggcaccacg gtcgcaccgg tgctcatcgc ggcgaagtcg
     1441 aagagggtcc attcgggcct ggtgttgccg atgatcgcga ccttgtcgcc acgctggacg
     1501 ccgagggcct tcagaccgag ggtgagcgcc tggacctccg ccgccacctt ttcgtaggag
     1561 gtctcctccc agctgccggc ctcgttcttg aagagaaagg caccgtccga tccgtgcctg
     1621 gcagccgagt tcggcagcag gtcagcgagg gttcgggaac ggttggtcgg cgtgtaggtg
     1681 ctggacaagt ctctctccta tgtggctatg tggcgacgca aagcggtttg atcgattgat
     1741 cctgccacat cagatcggat tgggcaggtc gaggaattca tgttcgacct cgaagcgttg
     1801 ggcgaccagg tcgccgaggg cggcaattcc gc
//
LOCUS       DAOQFI010000449         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40730_length_1831_cov_3.387387, whole genome shotgun sequence.
ACCESSION   DAOQFI010000449 DAOQFI010000000
VERSION     DAOQFI010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40730_length_1831_cov_3.387387"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLE93_07980"
     CDS             complement(<1..297)
                     /locus_tag="PLE93_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87026.1"
                     /translation="MTGLRRFALAGLCLGLLLAGCGGSDDQATIKPAPDRSARISQLE
                     QRKQNLELQLKAQRKKEAAEAEAKTAEVGGDADANALLTGLPGEAGLVVGAPGGG"
     gene            complement(294..788)
                     /locus_tag="PLE93_07985"
     CDS             complement(294..788)
                     /locus_tag="PLE93_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87027.1"
                     /translation="MSSLKGVSAVLALLVLGLFAYGCGGDDGATQEQLDAAKAEGAKE
                     ARQQARLNQLQGQVKQLQKQSKQSSNGNSGKTGGQGDGYYQDAGSDGGPVSTCATGVQ
                     VGPNTSCSFAMNVAGEYGSNPGASTISAYSPVTGEYYTMSCGSWSGGGTVCTGGNGAA
                     VYMP"
     gene            830..>1831
                     /locus_tag="PLE93_07990"
     CDS             830..>1831
                     /locus_tag="PLE93_07990"
                     /inference="COORDINATES: protein motif:HMM:NF025174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87028.1"
                     /translation="MNFAACRPSRASLPVFACLLIAAFVLPAFLPAPAEARFGQWLNF
                     GSSYGVPSGRIGIQFTRDVGLDESKATGNRPGGRVGFLSGGVWYHATRLADTVALPDP
                     SILNTNDPAGRQIRIEVRDIGNETMRLSAEVLGSGPEPVDSIGIGFGLKDSERLLGFG
                     ERSDHVNQRGNEVESYVGEGPYQETDYPIIKNTVPSWGVRERSDATYFPMPWFISTRG
                     YGVLAGNAETSRFRLGTESKGEWSFEADARRMTLRFFAGPRPAGVLRRMTRAIGRQPK
                     PRAPWIFGPWFQTGHQNTSPNELDYVETLRDADAPVSAVETHMRYMPCGSDLGQEAAD
                     "
BASE COUNT          289 a          653 c          578 g          311 t
ORIGIN      
        1 gccacccccg ggagcgccga cgaccaagcc cgcctcgccg ggcaggccgg tcagcagggc
       61 gttcgcgtca gcgtccccgc ccacctcggc ggtcttcgcc tcggcttccg ccgcctcctt
      121 ctttcgctgc gccttcagct gaagctccag gttctgtttg cgctgctcaa gctgcgaaat
      181 ccgggcgctg cggtcagggg cgggcttgat cgtcgcctgg tcatccgatc cgccgcaacc
      241 ggccaggagc agaccaaggc agagcccggc cagcgcgaac cgccgcaggc cggtcaaggc
      301 atgtagacgg cagcgccgtt tccaccggtg cagacggtgc caccgccgct ccaggagccg
      361 cagctcatcg tgtagtactc gccggtcacc gggctgtagg cgctgatcgt agaagcaccg
      421 gggttgctgc cgtactcacc ggccacgttc atcgcgaagg aacaggaagt gttcgggccg
      481 acctgcaccc cggtcgcgca ggtcgaaacc gggccaccgt ccgaaccggc gtcctggtag
      541 tagccgtcgc cctggccgcc tgtcttgccg gagttgccgt tcgagctctg cttcgactgt
      601 ttctgcagct gcttgacctg gccctgaagc tggttcagcc tggcctgctg gcgggcttcc
      661 ttggcgccct cggccttcgc cgcatcgagc tgctcctggg ttgccccgtc gtcaccaccg
      721 catccgtatg caaagaggcc gagaacgagc aaggcgagga cggcggatac tcccttgaga
      781 ctggacatga ccgaagctta gccctggtcg tagtgttctg gaagaactca tgaattttgc
      841 tgcctgccgt ccgtcacgcg cctccctgcc cgtattcgcc tgcctgctga tcgccgcttt
      901 cgtcctgccc gccttcctgc ccgcgccggc cgaagccagg ttcggccagt ggctcaattt
      961 cggctcctcc tacggggtgc cgagcggcag gatcgggatc cagttcaccc gtgacgtcgg
     1021 cctcgatgaa tcgaaggcga ccggcaatcg cccgggcggc cgggttggct ttctttcggg
     1081 cggtgtctgg taccacgcca cgcggctggc cgataccgtc gccctgcccg acccgtcgat
     1141 cctgaatacc aacgacccgg ccggccgcca gatcaggatc gaagtgcgcg acatcggcaa
     1201 cgagacgatg cggctgagcg ccgaagttct cggcagcggc ccggaaccgg tcgactcgat
     1261 cgggatcggg ttcggcctga aggacagcga gcggctgctc gggttcggcg agcgctccga
     1321 ccacgtgaac cagcgcggca acgaggtcga aagctacgtg ggcgagggcc cctaccagga
     1381 aaccgattac ccgatcatca agaacacggt cccgtcctgg ggagtgcgtg agcgctccga
     1441 cgcgacctac ttcccgatgc cctggttcat ctccacccgc ggttacggcg tgctcgccgg
     1501 caatgccgaa accagccgct tccggctcgg aaccgagagc aagggcgaat ggtccttcga
     1561 ggccgatgcc cgccggatga ccctgcgctt ctttgccgga ccccgaccgg ccggggtgct
     1621 gcggcgcatg accagggcga tcggccgcca gcctaagccg agggcaccct ggatcttcgg
     1681 cccctggttc cagaccggcc accagaacac ttcacccaac gaactcgatt acgtcgagac
     1741 cctgcgcgac gccgatgccc cggtctccgc ggtcgagaca cacatgcgct acatgccctg
     1801 cggttccgac ctcggtcagg aagcggccga c
//
LOCUS       DAOQFI010000450         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_40734_length_1831_cov_3.160473, whole genome shotgun sequence.
ACCESSION   DAOQFI010000450 DAOQFI010000000
VERSION     DAOQFI010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40734_length_1831_cov_3.160473"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLE93_07995"
     CDS             <1..542
                     /locus_tag="PLE93_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNH87029.1"
                     /translation="ADKAARFIATCNAFNIPLLFLADIPGFMIGTAVEKQGIIRHGAK
                     MISAMSEATVPKISVVVRKAYGAGLYAMAGPAFEPDSCIALPNASIAVMGPQAAVNAV
                     FYNQIQAIEDEAEREKFVEEKRKEYAEGVDLLHLASEMVIDAVIQPEDLRTELIKRFA
                     LAEGRDRSFTERRIPITPA"
     gene            564..1214
                     /locus_tag="PLE93_08000"
     CDS             564..1214
                     /locus_tag="PLE93_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNH87030.1"
                     /translation="MERSVWRYLFLAFLCASGTAALALLFYGSTRISEFDARVTSHFL
                     AVPDSRLDQVADFGARLAEPAPLILIAFGLIVLAVVWNRPWHLLAAGGVIIAANLTTQ
                     VMKALMAHPRLQGALGVSYPIEIHYPSGHTTAALASASGLWLVTPPRWRAWAAAFGAI
                     YALAVAAGVVIAGWHFISDVIGAALVVGFWACLAMAALVQAGLERTDRSRESSRTR"
     gene            complement(1189..>1831)
                     /locus_tag="PLE93_08005"
     CDS             complement(1189..>1831)
                     /locus_tag="PLE93_08005"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935946.1"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNH87031.1"
                     /translation="QARALGAAIESWDEEGNPHIGEVGELVITKPMPSMPLFLWGDED
                     GSRYHDSYFDTYPGIWRHGDWIEITERGTAIIYGRSDSTINRGGIRMGTSEIYRAVLA
                     DEDIVDALVVDIPREGTEGWMPLFVVLAEGAELTDELKKKVAGEIRSRCSPRHVPNEM
                     IEIKAVPRTLSGKVLEVPVKRILTGTPAGKAASRDSLANPEALDFFSEYAKTL"
BASE COUNT          274 a          618 c          586 g          353 t
ORIGIN      
        1 cggccgacaa ggcggctcgc ttcatcgcta cctgcaacgc cttcaacatc cccctgctct
       61 tcctggccga cattcccggt ttcatgatcg gcaccgcggt cgagaagcag ggcatcatcc
      121 gtcacggtgc caagatgatc tcggcgatgt ccgaggcgac agtgccgaag atctcggtcg
      181 tcgttcgcaa ggcctacgga gccggtctct acgcgatggc cggcccggcc ttcgagcccg
      241 attcctgcat cgccctgccg aacgcctcga tcgccgtgat ggggccgcag gcggccgtca
      301 acgcggtctt ctacaaccag atccaggcaa tcgaggacga ggccgagcga gagaagttcg
      361 tcgaggagaa gcgcaaggaa tacgccgaag gggtggacct gctccacctc gcttccgaga
      421 tggttatcga cgcggtgatc cagcccgaag acctccgcac ggaactcatc aagcgcttcg
      481 ccctggccga gggccgtgac cgctccttca ccgagcgccg cattcccatc acccccgcct
      541 gaaggtttcc gtagggtttc cggatggagc gatccgtctg gagatatctg ttcctcgcgt
      601 ttctctgcgc cagtggaacc gccgctctgg cgctgctgtt ttatggctca accaggatca
      661 gcgagttcga tgcccgggtc acttcccact tcctcgccgt gccggactcc cggctcgacc
      721 aggttgccga cttcggggcg aggctggccg agccggctcc cctgatcctg atcgcatttg
      781 gcttgatcgt tctcgccgtc gtctggaacc ggccctggca cctgctcgcg gccggcgggg
      841 tgatcatcgc cgccaacctg acgacccagg tgatgaaggc gctgatggcc catccccggc
      901 tccagggggc gctcggggtc agctacccga tcgagattca ctacccgagc ggtcacacca
      961 ccgccgcgct cgcctcggct tccggcctct ggctggtgac gccgccacgc tggcgggcct
     1021 gggctgcggc tttcggggcg atctacgcgc tcgctgtggc cgccggcgtg gtgattgccg
     1081 gctggcactt catcagcgac gtgatcgggg cggcactggt ggtcggcttc tgggcctgcc
     1141 tcgccatggc cgccctggtc caggccggcc ttgaacgaac cgatcgctct agagagtctt
     1201 cgcgtactcg ctgaaaaagt cgagcgcttc ggggttggcc agtgagtcgc ggctggccgc
     1261 cttcccggcc ggggtgccgg tgaggatccg cttgaccgga acttcgagaa ctttgcccga
     1321 aagggtgcgg gggaccgctt tgatctcgat catctcgttg ggaacgtgtc gcggggagca
     1381 gcggctgcgg atttcgccgg cgactttctt cttcaactcg tcggtcagtt cggccccctc
     1441 ggccaggacg acgaagaggg gcatccagcc ttcggttcct tcgcggggga tgtcgaccac
     1501 gagggcatcg acgatgtcct catcggccag taccgcccgg tagatctccg aggtacccat
     1561 gcggatgcca ccccggttga tggtcgagtc ggagcggccg tagatgatcg cggttccccg
     1621 ctcggtgatc tcgatccagt cgccgtgccg ccagattccc gggtaggtgt cgaagtagct
     1681 gtcgtggtag cgggatccgt cctcgtcgcc ccagagaaac agcggcatcg acggcatcgg
     1741 cttggtgatg accagttccc cgacctcgcc gatatgcggg ttgccctctt cgtcccagga
     1801 ctcgatcgcc gccccgagcg cccgggcctg c
//
LOCUS       DAOQFI010000451         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_41006_length_1824_cov_3.398530, whole genome shotgun sequence.
ACCESSION   DAOQFI010000451 DAOQFI010000000
VERSION     DAOQFI010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41006_length_1824_cov_3.398530"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..636
                     /locus_tag="PLE93_08010"
     CDS             160..636
                     /locus_tag="PLE93_08010"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH87032.1"
                     /translation="MPSIHTTELIDASPDRVWQILADLSSYPDWNPFIIEASGELREG
                     EKLVLRMQPPGGKAMNFRPTVLKADPGQELRWLGRLGFRGIFDGEHYFRLTPEGGGTR
                     LDQGEEFTGLLPRFMGKVLSQTERGFEALNAALRKRAERDVEAPAGLAPEKETAGS"
     gene            633..1112
                     /locus_tag="PLE93_08015"
     CDS             633..1112
                     /locus_tag="PLE93_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HNH87033.1"
                     /translation="MSRRAEITAAARSILEQEGEEALTMRNLADRLGIKAPSLYKHVS
                     SRREIETLLAAEALNEMRESLAAADGLEEMGRSYRAWAIANPSLYRIATTRPLDRENL
                     PAGLEDAAAAPLLAAVDGDRARARAFWALAHGLVLLELDGRFPGDADLEAAWLAGIS"
     gene            complement(1109..1501)
                     /locus_tag="PLE93_08020"
     CDS             complement(1109..1501)
                     /locus_tag="PLE93_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH87034.1"
                     /translation="MSSLIHTCYRVFDLDESIDFYKKLGLLEHHRLPIGDEAVNVYMG
                     HEGDGARLELTFNYDQQEPYEIGTGYGHIAFVVDDLDSTLEGLADQGIAPEKPPYTVS
                     EGGSRLCFVKDPDGYRIELIEGDPGRAD"
     gene            1538..>1824
                     /locus_tag="PLE93_08025"
     CDS             1538..>1824
                     /locus_tag="PLE93_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87035.1"
                     /translation="MSTYERVAELPVKIESWSMEGLSIKPSPEFERLTTVIRLEGAGQ
                     TGLGEDITYEALDQIALQDAGATHDLTGPNTVGEFCELIGGLDLFPSPPER"
BASE COUNT          310 a          609 c          597 g          308 t
ORIGIN      
        1 acgggtcgat cgacggctac atgcgggacg gactcggcct cgaagatggc actctgctcg
       61 ccctgcggcg ccgcctgctc gatacctgag gcgcctgctt cgaggcgata cggccaacag
      121 tgtttgtctg cgctggctaa ctgtgttagc ctcgggctca tgccttcgat ccacacaacc
      181 gaattgatcg atgcctcgcc cgaccgcgtc tggcagatcc tggccgacct ttcctcctac
      241 ccggactgga acccgttcat catcgaagcc tcgggcgaac tccgggaagg cgaaaagctt
      301 gtgctcagga tgcagcctcc cgggggcaag gcgatgaact tcaggccgac ggtgctcaag
      361 gccgatcccg gccaggagct gcgctggctg ggccggctcg ggttccgggg catcttcgac
      421 ggcgagcact actttcgcct caccccggag ggcggcggga ccaggcttga tcagggcgag
      481 gaattcaccg gcctcctccc ccgcttcatg ggcaaggttc tcagccagac cgagcgggga
      541 ttcgaagctt tgaacgcggc gctcaggaag cgggccgaac gcgacgtcga agcgccggcc
      601 ggccttgcgc cggagaaaga aacggccggc agttgagccg gcgcgcagag atcaccgccg
      661 cggcccggtc gatcctcgag caggaaggcg aggaggccct gaccatgcgc aacctcgccg
      721 accgtctcgg catcaaggct ccctcgctct acaagcacgt gagcagccgt cgggagattg
      781 aaacgctgct ggccgccgag gccctcaacg agatgcgtga atcactcgcc gcagccgacg
      841 gcctggaaga gatgggccgt tcctaccggg cctgggccat cgccaacccg tcgctctacc
      901 ggatcgccac cacccggcca cttgaccgcg agaacctgcc ggccgggctc gaggacgccg
      961 ccgcggcacc tctgctcgcg gcagtcgacg gcgaccgggc acgggcccgc gccttctggg
     1021 ccctggcgca cggcctggtg ctgctcgaac tggacggccg gttccccggg gacgccgacc
     1081 tcgaagccgc ctggctggcg gggatttcct agtcggcgcg acccgggtcg ccttcgatca
     1141 gttcgatccg gtagccgtcg gggtctttca cgaagcagag cctcgacccg ccctcgctga
     1201 ccgtgtaggg aggcttttcg ggtgcgatgc cctggtctgc gaggccctcc agggtgctgt
     1261 cgagatcatc gaccacgaac gcgatgtgcc cgtagccggt gccgatctcg tagggctcct
     1321 gctggtcgta gttgaaggtc agctcgagtc gggccccgtc gccttcatgg cccatgtaga
     1381 cgttcaccgc ctcgtctccg atcggcaggc gatgatgctc cagcagaccg agcttcttgt
     1441 agaagtcgat cgactcgtcg aggtcaaaga cgcggtaaca ggtatggatc agggaggaca
     1501 tgaatggagc ctacccgccc tcagtaggct caaccccatg agcacctacg agcgtgttgc
     1561 ggagttgccg gtgaagatcg aatcgtggtc gatggagggc ctttcgatca aaccttcgcc
     1621 ggagttcgag cggttgacga ccgtgatccg cctcgaaggg gccggccaga ccggactggg
     1681 cgaggacatc acctacgagg cgcttgacca gatcgccctt caggatgccg gggccactca
     1741 tgacctgacc gggccgaaca cggtcggcga gttctgcgag ctgatcggtg gccttgacct
     1801 cttcccctcc ccgcccgagc gtga
//
LOCUS       DAOQFI010000452         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_41008_length_1824_cov_3.192764, whole genome shotgun sequence.
ACCESSION   DAOQFI010000452 DAOQFI010000000
VERSION     DAOQFI010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41008_length_1824_cov_3.192764"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLE93_08030"
     CDS             <1..326
                     /locus_tag="PLE93_08030"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH87036.1"
                     /translation="EIEVTMVAEEGNPKSAMKINACEPGRSYEVTGSDESGGWNLELK
                     LEEGEGGTRLTFIHHLGEEDKVEMIGPGWEFYLDHLLAAIEGNEKKDFSEYWPAMGPY
                     YAELG"
     gene            complement(328..401)
                     /locus_tag="PLE93_08035"
     tRNA            complement(328..401)
                     /locus_tag="PLE93_08035"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(365..367),aa:Asp,seq:gtc)
     gene            570..1418
                     /locus_tag="PLE93_08040"
     CDS             570..1418
                     /locus_tag="PLE93_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNH87037.1"
                     /translation="MRLVTYASERGPRAGVQTDQGVIDAWDLVDGEGASLRELIASGR
                     LDEVRTALDGGGGEPVMLSPGGILAPVPDPDKIICVGLNYRSHAEETGMELPKAPTIF
                     GKYRNALVPDGAKVTLPAASSKCDYEAEVAFVIGRRAREVSVEDALDHIAGYTLLNDL
                     SARDLQGLTMQWMAGKVFDGSAPCGPALVTPDEAGPHDGIGIRMLVNGEERQSSNTSD
                     LIFSVPQLVSTLSSWMTLEPGDIVATGTPSGVGLAMDPRTWLEDGDELVVSSPQLGEL
                     HTTIAR"
     gene            complement(1424..>1824)
                     /locus_tag="PLE93_08045"
     CDS             complement(1424..>1824)
                     /locus_tag="PLE93_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNH87038.1"
                     /translation="KILARPVSRVSGYRAYSGHVTLEKGDRGDQVLWLQQHLIAAGYS
                     MEPTGIFSRSTYKAVRRFQARRGLPADGVVGTRTWNRLMRIRPVRVRWSAAHRRNKAA
                     GASASSAPLSAPLSADLPPVRNEIAGAKHP"
BASE COUNT          332 a          585 c          619 g          288 t
ORIGIN      
        1 gtgaaatcga ggtgaccatg gtcgccgagg agggcaaccc gaagtcggcg atgaagatca
       61 acgcctgcga gccgggccgc tcctacgagg tgaccggctc ggatgagtcc ggcggctgga
      121 acctggaact gaagctggag gaaggggaag gcgggaccag gctgaccttc atccaccacc
      181 tcggtgagga agacaaagtc gagatgatcg ggccgggctg ggagttctat ctcgaccacc
      241 tgctggcggc aatcgaggga aacgagaaga aggacttcag cgagtactgg cccgctatgg
      301 ggccgtacta cgcggagctt ggatgaacgg gagcgacggg acttgaaccc gcggcctctg
      361 gcgtgacagg ccagcgctct aaccaactga gctacgcccc cgtgaggcga tcggtcgaaa
      421 ccgaccgggc gggcattatc gcagtctcga acgaacggcg acatctgacc cttcccgatt
      481 cccgttccgg gggcgtttac ccaccgtttg tgtggtcaat cgcccccggt tgccgatcgt
      541 ttccggcgtt cgggttctag actcgcgcca tgagactggt cacgtatgca tcggagcggg
      601 gcccgcgggc tggagtccag accgaccaag gcgtgatcga cgcctgggac ctggttgacg
      661 gcgagggcgc aagcctcagg gaactgattg cctccggccg cctcgacgag gtccggaccg
      721 cgctggacgg gggcggtggc gaacccgtga tgctgagccc gggcggaata ctcgcgccgg
      781 ttcccgatcc ggacaagatc atctgcgtcg ggctgaacta ccgctcgcac gccgaggaga
      841 ccggcatgga actgccgaag gccccgacca tcttcggcaa gtaccgcaat gccctggtcc
      901 cggacggagc gaaggtgacc ctgccagccg cgagcagcaa gtgtgattac gaggcagagg
      961 tcgcctttgt gatcggccgg cgggcaagag aggtcagcgt cgaagatgcg ctcgaccata
     1021 tcgccggcta cacccttctc aacgaccttt cggcccgcga cctgcagggc ctgaccatgc
     1081 agtggatggc cggcaaggtc ttcgacggct cggctccctg cggccccgcc ctggtcactc
     1141 cggacgaggc cgggccgcat gacgggatcg gcatcagaat gctggtcaac ggcgaggagc
     1201 gccagagctc gaacacctcc gacctgatct tcagcgtccc gcaactggtc tcgacccttt
     1261 cgagctggat gaccctggag ccgggcgaca tcgtcgccac cggaacccct tccggggttg
     1321 gcctggcgat ggacccgcga acctggcttg aagacggaga cgaactggtc gtcagctcgc
     1381 cacagctggg cgagcttcac accacgatcg cccgctagca ggctcagggg tgcttggcgc
     1441 cggcgatttc gtttcggacc gggggcagat cggcggagag cggtgcgctg agcggtgccg
     1501 atgaagcgga cgccccggcg gccttgttgc ggcggtgggc agctgaccac ctgaccctga
     1561 ccggacggat ccgcatcagg cgattccagg tccgggttcc gaccacgccg tcggccggca
     1621 gaccccgcct ggcctggaac cggcggactg ccttgtaggt gctgcgactg aagatcccgg
     1681 tcggctccat cgagtagccg gccgcgatca gatgctgctg cagccagagc acctggtcac
     1741 cgcgatctcc cttctcaagc gtcacatgac ccgagtaggc ccggtaaccg gacacccggc
     1801 tcaccggcct ggcgaggatc ttcc
//
LOCUS       DAOQFI010000453         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_41013_length_1824_cov_2.765969, whole genome shotgun sequence.
ACCESSION   DAOQFI010000453 DAOQFI010000000
VERSION     DAOQFI010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41013_length_1824_cov_2.765969"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..718)
                     /locus_tag="PLE93_08050"
     CDS             complement(260..718)
                     /locus_tag="PLE93_08050"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HNH87039.1"
                     /translation="MKKLAITAVLLICGLTASSALAADEDQVDPDITSGKAAREFRQA
                     RQKWLAAGIVDYRMKVSRGCFCAGPSSVTITVRGGKAVKVSDKPWYGPWTVPGMYRII
                     GQAIKNEVAALDVTYGAKLGIPRQTSIDYIAMAVDDEIGYGISGFKALKP"
     gene            767..1750
                     /locus_tag="PLE93_08055"
     CDS             767..1750
                     /locus_tag="PLE93_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNH87040.1"
                     /translation="MPYLHKWSPALVRTLPHTRLGTGPTPVRPLPSLGQSGCEIWLKD
                     ESGFGDGGWGGNKVRKLEWLLPDVKRRGRKTILTFGGLGTNWGLATALYGREQGIHTA
                     LGLIDQPRDQHVEEQLKRLEASGASLHFTHTKNRTIALAPWLILRHSSGLRPPYILPS
                     GGSSPLGALSYVETAFEIAAQVESGELPEPGHAVVPTGSGGTAAGLALGFALAGLGTR
                     VVGVIVTEHLRLDDEAIGKLAGKSAALLRQRGAAFPEPDLNFEMTEEWLGETYGAPTP
                     ESEAAIERARADGLELDPVYTAKAMAALIAKSAAGDFEGGPVLFINTNGPR"
BASE COUNT          306 a          649 c          586 g          283 t
ORIGIN      
        1 gtttcagcag aaaggtctgc ggcgccgggc ggtcggcctg aacacgggcg gcggaagcgc
       61 tctggcctgc ggctccagcg aacacggcca gcgccaatcc accgatgacg gcgccccacg
      121 cccttttacc ccacccgctg ctcttcatgc cgccgacctt tgctcgaatt cagatgcctg
      181 acgggtcaga ctgcgacccg gaccgccgtg agtctaaacc ccgctccggg ccgaaagtgg
      241 cggtctgcgc cgcgctggct cagggcttca gcgccttgaa gccggagatc ccgtagccga
      301 tctcgtcgtc gaccgccatc gcgatgtagt caatcgaggt ctgcctgggt atgcccagct
      361 tcgcgccgta ggtgacgtcg agcgcggcga cttcattctt gatcgcctgg ccgatgatcc
      421 ggtacatgcc gggcacggtc cacggcccgt accagggctt gtccgagacc ttgactgcct
      481 tgccaccacg aacggtgatg gtcacgctgg aaggaccggc gcaaaaacag ccccgggaaa
      541 ccttcatccg gtagtccacg atgcccgcgg caagccactt ctgccgggcc tgcctgaact
      601 ccctggccgc cttgccgctg gtgatgtccg ggtcaacctg atcctcgtcg gcggcgaggg
      661 ccgagctggc cgtcaggccg caaatcagga gaacggcggt gatggcgagt ttcttcatgg
      721 ccatattctt actacgaaac cgatcggcaa ccgtcacaat cgcgccgtgc cctatctcca
      781 caagtggagc cccgccctgg tgcgaaccct cccccacacg cgactcggca ccggaccgac
      841 gccggtcagg cccctgccct ccctcgggca aagcggctgc gagatctggc tcaaggacga
      901 aagcggcttc ggtgacggag gctggggcgg caacaaggtc cgcaaactcg agtggctcct
      961 tcccgacgtc aaacggcgcg gccggaagac gatcctgacc ttcggcggcc tcggcaccaa
     1021 ctggggcctc gcgaccgcgc tctacgggcg cgagcaggga atccacacgg ccctcgggct
     1081 gatcgaccag ccccgcgacc agcacgtcga ggagcagctg aagcgcctgg aagcctccgg
     1141 ggcaagcctc cacttcaccc acacgaagaa ccgcacgatc gccctcgccc cctggctgat
     1201 cctccgccac tcttccggcc tgcggccgcc gtacatcctg ccctcgggag gttcttcgcc
     1261 gctcggtgcg ctcagttacg tcgagacggc gttcgagatc gcggcgcagg tcgagtcggg
     1321 tgagttgccc gaaccgggcc acgcagtggt gccgaccggg tccggcggaa ccgcggcggg
     1381 cctggcgctc ggcttcgccc tcgccgggct cggcacccgg gttgtcggcg taatcgttac
     1441 cgagcacctt cggctcgatg acgaagcgat cggcaagctg gccggcaaga gcgctgccct
     1501 gctccgccag cgcggggccg cgttccccga gccggacctg aacttcgaga tgaccgagga
     1561 gtggctcggg gaaacctacg gcgcccccac cccggaatcg gaggcggcga tcgaaagggc
     1621 ccgggccgac gggctcgaac tcgacccggt ctacaccgcc aaggcgatgg ccgccctgat
     1681 cgccaagtcc gcggccggtg acttcgaagg cgggcccgtc ctcttcatca atacaaacgg
     1741 gccccgttaa ccgaagaggg tgaggtcggg ttcgcgttcg ccgcggagga ctgcgaggtc
     1801 gacttcgacg caggcgatgt cccg
//
LOCUS       DAOQFI010000454         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_41107_length_1822_cov_2.629315, whole genome shotgun sequence.
ACCESSION   DAOQFI010000454 DAOQFI010000000
VERSION     DAOQFI010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41107_length_1822_cov_2.629315"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1725
                     /locus_tag="PLE93_08060"
     CDS             223..1725
                     /locus_tag="PLE93_08060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNH87041.1"
                     /translation="MAPRKTEHPSGSTSRQNGRANGASGDAGAESAGGKALPESETKA
                     GSSESVLRPPGSGTRDRTAREASKGRTKGSRRIALPRLKGRKTKKAAGSGAAPTTAAS
                     GSATSRGKAATGNGNGQPLSIPSAPGVGGNGNGTGNGNGNGTGLPPGVLTLPVPPPRP
                     PKPKIKKLRVLFIVLGLGLLGFCSAFFGMVMAVSQDLPAIENYAQFRTAKNSILVDSQ
                     GKYIGTLSNNENQMLLDAEQISPNMKNAVVSIEDARFYEHRGIDFQGLGRALVQDIIA
                     RSAKQGASTITQQLVKQALEAQNDRSPLQKVREMSIAYHIEQQWTKDKILTEYLNTVY
                     FGNGAYGIEAAARTYFGYAHPSCGTDEEPCASLLTPAESALLAGIIQNPYGYDPKNDP
                     DAALYRRNTVLDRMLDQGYITQEQHDEAVKEALPADDDIDPPSLDSKAPYFTSWMRQQ
                     LVDRYGAGRAFFGGLKVKTTIDLDVQAATEDAIYSTIGGIGPSASVVITS"
BASE COUNT          362 a          620 c          570 g          270 t
ORIGIN      
        1 gccggccctc gggcagggac tcaaccgcgg tccgggtcag cgccgcggcc tgctcgactg
       61 cttctgctga gggttcgccg ggttcttgag tgacgtttga cacgggagag tcaatgctag
      121 actgagcagc gctacttggt cgtgtgcgcg ttgttcatga aaggcgtgca gcgcatccca
      181 cgacggcttt cgccaaggcg ctgacctact tttcgccatc tgatggcccc ccgaaagaca
      241 gagcatccgt cgggatcgac ttcccggcaa aacggacgcg ccaacggtgc gtcaggtgac
      301 gcaggcgcag aaagcgccgg cggcaaggcg ctgcccgaat ccgaaacgaa ggcgggcagc
      361 agcgaaagcg tgctccggcc gccaggcagc ggcacccgtg accgcacggc cagggaggca
      421 tcaaagggtc gcaccaaagg ttcccgccgg atcgcactgc ccaggcttaa gggccggaag
      481 accaaaaagg ccgccggctc cggtgccgcc ccgaccacgg ccgcctcggg gtccgcgacc
      541 agcagaggca aggccgcgac cggaaacgga aacgggcagc ccctctcgat cccgagcgct
      601 cccggggtcg gcggaaacgg gaacggaacc ggcaatggaa acgggaacgg aaccggcctg
      661 ccgcccggcg tgctcacgct cccggtacct ccgccccggc cgccaaaacc gaagatcaag
      721 aagctccggg tcctcttcat cgtgctcggt ctcggcctgc tcggtttctg ctcggccttc
      781 ttcggcatgg tcatggccgt ttcgcaggat ttgccggcga tcgagaacta cgcgcagttc
      841 cggaccgcca agaactcgat tctcgtcgac agccagggca agtacatcgg caccctgtcg
      901 aacaatgaga accagatgct gctcgacgcg gagcagatct cgccgaacat gaagaacgcg
      961 gtggtctcga tcgaggacgc gcgcttttac gaacaccgcg gaatcgactt tcagggtctc
     1021 ggacgggcgc tcgtacagga catcatcgcc cgcagcgcga agcagggcgc ctcgaccatc
     1081 acccagcagc tcgtaaagca ggcactcgaa gcccagaatg accgctcgcc gctccagaag
     1141 gtgcgcgaga tgtcaatcgc ctaccacatc gaacagcagt ggaccaagga caagatcctc
     1201 accgagtacc tgaacacggt ctacttcggc aacggtgcct acggaatcga ggcggccgcc
     1261 cggacctact tcggttacgc ccacccctcc tgcggcacgg acgaagagcc ctgcgcctcg
     1321 ctgctcaccc cggctgaatc ggcgctgctc gccggcatca tccagaaccc ttacggctac
     1381 gaccccaaga acgaccccga tgcggcgctc taccggcgca acaccgtgct cgaccgcatg
     1441 ctggatcagg ggtacatcac ccaggagcag cacgacgaag cggtcaagga ggccctgccg
     1501 gccgacgatg acattgaccc gccgagcctc gattcgaagg cgccctactt cacctcctgg
     1561 atgcgccagc agctggtcga ccgctacggc gccgggcgtg cgttcttcgg tggcctcaag
     1621 gtcaagacga cgatcgacct tgacgtccag gccgcaaccg aggacgccat ctacagcacc
     1681 atcggtggaa tcggtccgag cgcctcggtc gtgatcacga gctgacgtac ttcgagttcg
     1741 gcaccgcgct ggcgttctgg cacttcgcgc gggagcaggt cgacatcgcg gtcatcgagg
     1801 tcgggctggg cgggcgcctg ga
//
LOCUS       DAOQFI010000455         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_41295_length_1818_cov_1.990925, whole genome shotgun sequence.
ACCESSION   DAOQFI010000455 DAOQFI010000000
VERSION     DAOQFI010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41295_length_1818_cov_1.990925"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..817)
                     /locus_tag="PLE93_08065"
     CDS             complement(98..817)
                     /locus_tag="PLE93_08065"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNH87042.1"
                     /translation="MGLLGVIGGSGLYGLSASGRPKAVSTPWGPASGPTVIHEVGGGV
                     AFIARHGADHSIAPHRVNYRANIHALREAGCDRVLAVSSVGGIAPACQPGKLVVPDQL
                     IDYTWGREMTFQGEGDAVRHFDFTDPYSPDWRGKVTGALSELEIEFVDGGTYAAVQGP
                     RFETAAEIRRLAADGCHIVGMTGMPEAILAREAGLDYAAVCPVGNLAAGIATEELSMD
                     DVIGAVGSGMERVTSLITNLA"
     gene            complement(831..>1818)
                     /locus_tag="PLE93_08070"
     CDS             complement(831..>1818)
                     /locus_tag="PLE93_08070"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNH87043.1"
                     /translation="NLAMLIPTMEGWTVETVGDDIAWMKFGEEGRLRAVNPEAGFFGV
                     APGTGADSNPNAMATIVDNTVFTNCAKTDDGDIWWEGIGPAPEHLIDWHGNDWAPDSE
                     TPAAHPNARFAVPAAQCPTIASEWEDPAGVPIDAFLFGGRRASVVPLVSESFDWNHGV
                     YLAATMSSETTAAAAGAVGKLRFDPMAMLPFCGYNMADYFGHWLDIGQTEGAKLPKIF
                     YVNWFRKDEDGKFIWPGFGDNSRVLEWVFNRCDDRGETVETSIGLLPKVEELDLEGVD
                     VTPEQMEVLLTVDDEAVKAQLPQIEEHLDKFGDSLPAEIRNQFEALKGRLGA"
BASE COUNT          302 a          605 c          606 g          305 t
ORIGIN      
        1 agcgcggctg aatgccgcgc gtgcgtccgt cccggagatc ctcacccggc cccgcggcgc
       61 aaacacagcg accccgcggc gtcgccacca gagctggtca ggcgagattt gtgatcaggc
      121 tggttacccg ttccatgccg gagccgacgg cgccgatcac gtcgtccatg ctgagttcct
      181 cggtggcgat gccggccgcg aggtttccaa ccgggcagac ggccgcgtag tctaggccgg
      241 cctcgcgggc gaggatggct tcgggcatgc cggtcatgcc gacgatgtgg cagccgtcgg
      301 cagcgaggcg ccggatttcc gcggcggttt caaaccgcgg tccctggacc gcggcgtaag
      361 tgccgccgtc gacgaattcg atctccagct cgctcaacgc cccggtgacc ttcccgcgcc
      421 aatcgggtga atagggatcg gtgaaatcga aatggcggac cgcatcgccc tcaccctgga
      481 aggtcatctc gcgaccccag gtgtaatcga tcagctgatc ggggacgaca agctttcccg
      541 gctgacaggc cggagcgatc ccgccgacgc tggaaacggc gaggacccgg tcgcagccgg
      601 cctcacgcag ggcgtggatg ttggcccggt aattgacccg atgcggggcg atcgaatgat
      661 cggccccgtg gcgggcgatg aacgcaaccc cgcctccgac ctcgtgaatg accgtggggc
      721 ccgatgccgg gccccacggg gttgaaacag ccttgggccg gcctgaggcg ctcaggccgt
      781 agagaccgct gccgccgatg accccgagca ggcccacggc agttctgccg tcaggctccg
      841 agccggccct tgagggcttc gaactgattc cggatctcgg ccggcagtga atcgccgaac
      901 ttgtccagat gctcttcgat ctggggcaac tgtgccttga ccgcttcgtc gtccacggtc
      961 agcagcactt ccatctgttc cggggtcacg tcgacgcctt cgagatcgag ttcctcgacc
     1021 ttaggcagca gcccgatcga ggtctcgacc gtctcgcccc ggtcgtcgca gcggttgaag
     1081 acccactcga gcacgcggct gttgtcgccg aatccaggcc agatgaactt gccgtcttcg
     1141 tccttgcgga accagttgac atagaagatc ttcggcagct tcgccccctc ggtctggccg
     1201 atgtcgagcc agtggccgaa gtagtcggcc atgttgtacc cgcagaaggg cagcatcgcc
     1261 atcgggtcga agcgcagctt gccgaccgcg ccggcagcag ctgccgtggt ctcggaagac
     1321 atggttgcgg cgaggtaaac gccgtgattc cagtcaaagg actcggaaac gagcggcacc
     1381 acactggccc ggcggccacc gaagaggaag gcgtcgatcg gcacgccggc cggatcctcc
     1441 cattcggagg cgatggtggg gcactgcgcg gcaggaacgg cgaagcgggc gttcgggtgg
     1501 gctgccgggg tctccgaatc aggggcccag tcattgccgt gccagtcgat caggtgctcg
     1561 ggagcggggc cgatcccctc ccaccagata tcgccatcgt ccgtcttcgc gcagttggtg
     1621 aagaccgtgt tgtcgacgat cgtcgccatc gcgttcgggt tcgagtcggc accggtgccg
     1681 ggggcgaccc cgaagaagcc ggcctcggga ttgacggcgc gcaggcggcc ctcctcgccg
     1741 aacttcatcc aggcgatgtc gtcgccgacc gtctccacgg tccagccctc catcgtcgga
     1801 atcagcatcg cgagattg
//
LOCUS       DAOQFI010000456         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_41374_length_1816_cov_2.168654, whole genome shotgun sequence.
ACCESSION   DAOQFI010000456 DAOQFI010000000
VERSION     DAOQFI010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41374_length_1816_cov_2.168654"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLE93_08075"
     CDS             complement(<1..537)
                     /locus_tag="PLE93_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNH87044.1"
                     /translation="MSEQLWGGETTKAIENFPVSGEKIPPSLVKWLGLIKANAARANA
                     ELGLLDTDIADRIAKAGDEVGAGRHDDQFPIDIFQTGSGTSSNMNANEVIANLAGEGV
                     HQNDHVNMGQSSNDVFPSAVHLAALDQVTHDLLPAMEQLAVALEAKAEEFRDVVKAGR
                     THLMDAVPVTLGQEFGGYA"
     gene            complement(607..1119)
                     /locus_tag="PLE93_08080"
     CDS             complement(607..1119)
                     /locus_tag="PLE93_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87045.1"
                     /translation="MRRTLVISLILPALALPFAGCSDGRKVATPPACLSASATWLTAL
                     SSAPDQVLLEGVTPLSDCLTKGQSAAQHEETGKTAVETATQLAAFYKAGEKAGGNGTG
                     PGGDTSEQAAVMAGYLVGAIEKGAEDTEGIHAALVDRVEAAATNGLEGASQQIQGAYQ
                     KGYAAGQAKG"
     gene            1203..>1816
                     /locus_tag="PLE93_08085"
     CDS             1203..>1816
                     /locus_tag="PLE93_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNH87046.1"
                     /translation="MPTVREATFDLFRKLGMTTIFGNPGSTELPMLADYPDDFEYVLG
                     LQEAAVVGMADGHAQVTGKPTLVNLHTAPGLGNAVGALFNAQANHSPVVVTVGQQVRA
                     QVTMQANLTNRDAIRVGDPFVKYSYEPARAEDVPLAIAHAIHLASLPPMGPVVVSIPM
                     DDWDVEMNAAEAQHVIDRAVVGRSGADPPAPKTCRWRSPTRFTSR"
BASE COUNT          271 a          627 c          583 g          335 t
ORIGIN      
        1 ggcgtagcca ccaaactcct ggccgagggt gaccggcacc gcgtccatca ggtgggtgcg
       61 gccggccttg accacgtccc tgaattcctc ggctttggct tcaagcgcaa ccgccagctg
      121 ttccatcgcc ggcagcaggt cgtgggtgac ctgatcgagg gcagcgaggt gaacggccga
      181 ggggaagacg tcatttgacg actggcccat gttcacgtgg tcgttctggt ggactccttc
      241 gccggccagg ttggcgatca cctcgttcgc attcatgttc gaggaggtgc cggaaccggt
      301 ctggaagatg tcgatcggga actggtcgtc atgcctgcct gctccgacct cgtccccggc
      361 cttcgcgatc cggtcggcga tgtcggtgtc gagcagtccc agttccgcgt tggcccgggc
      421 ggcgttggcc ttgatcagcc ccagccactt gaccagcgag ggcggaatct tctcgccgga
      481 cacggggaaa ttttcgatcg ccttggtggt ttcgccaccc cagagttgct cgctcacttc
      541 tgtccttccg gctttgactg acttgctgcg gacagtcttt cagcccggca ccggaacggc
      601 gttcgcctag cccttcgcct gaccggccgc gtaacccttc tggtaggcgc cctgaatctg
      661 ctggctggcc ccttcgagcc cgttggtggc ggcggcctcg acccggtcga ccagagcggc
      721 gtgaattcct tcggtgtctt cggccccctt ctcgatcgcc ccgaccaggt aaccggccat
      781 caccgccgcc tgttcggagg tgtcgccacc gggcccggtc ccgttgccgc ccgccttttc
      841 gccggccttg tagaaggcgg ccagctgggt ggccgtctct accgcggtct tgccggtttc
      901 ttcatgctgg gccgcggatt gccccttggt caggcagtcg ctgagcggcg tgaccccttc
      961 gagcaggacc tgatccggtg ccgaagaaag tgccgtcaac caggtcgcgg aggcgctcag
     1021 acaggcgggc ggcgtcgcga ccttgcgacc gtcgctgcag ccggcgaagg gcagggcgag
     1081 ggcgggaagg atcagactga tcacgagagt tctgcgcacg gcacaatcct gacaggccag
     1141 gaccttcgcc ccacccttgc ctgccggtcc cgtactcacc cgttcacata taggctgcgc
     1201 ccatgcctac cgttcgcgaa gcgaccttcg accttttccg caagctgggg atgaccacga
     1261 tcttcggtaa cccgggctcg accgagctgc cgatgctggc cgattacccc gatgacttcg
     1321 agtacgtgct gggcctgcag gaggcggccg tggtgggcat ggccgacggt cacgcccagg
     1381 tcaccggcaa gccgaccctg gtcaacctcc acaccgcccc gggtctcggc aacgcggtcg
     1441 gcgcgctctt caacgcgcag gcaaaccact cgccggtggt ggtcacggtc ggccagcagg
     1501 ttcgcgccca ggtcacgatg caggcgaacc tgaccaaccg cgacgcgatc cgcgtcgggg
     1561 accccttcgt caagtactcc tacgaacccg cccgcgccga agacgtgccg ctggcgatcg
     1621 cccacgcgat tcacctcgcg tcgcttccgc cgatggggcc ggtcgtcgtt tcgattccga
     1681 tggacgactg ggatgtcgag atgaacgcag ccgaggcaca gcatgtgatc gaccgggcag
     1741 tggtcggacg atcgggcgcc gacccgcccg cgccgaagac gtgccgctgg cgatcgccca
     1801 cgcgattcac ctcgcg
//
LOCUS       DAOQFI010000457         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_41638_length_1810_cov_3.384046, whole genome shotgun sequence.
ACCESSION   DAOQFI010000457 DAOQFI010000000
VERSION     DAOQFI010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41638_length_1810_cov_3.384046"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLE93_08090"
     CDS             complement(<1..701)
                     /locus_tag="PLE93_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87047.1"
                     /translation="MSSPGRFGCVLATVLAGLMVWAAPSGAIPWDLGGAVPPEETTAP
                     VLPDPLCQQSYADDEPVPGPRIHFGVGPRLAGEIGSGQTTPLVPENWQERDRALRRLA
                     GDRDFTVRLNRLFLSDGWRGIRQFQKMARHYGRLGFGVELQVRYHPRPQQNGDIGAWL
                     DYVRDVVRAFGPIKAVRALQITNEVNLTVSPNTSDGAWLNSTRALVAGVKTAQRYSNR
                     LGFGHLKIGFNFAWR"
     gene            complement(698..>1810)
                     /locus_tag="PLE93_08095"
     CDS             complement(698..>1810)
                     /locus_tag="PLE93_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNH87048.1"
                     /translation="PGMLSDSLPDRFGNALIDAWLARQGREPGSMGAVERLCYTGRRG
                     MGALEYLPATGPEPVESHPVEVEALAELAAEILAERSGIRESFASEDRERALGEILRV
                     GTSAGGARAKALLAFNPETMEVRSGQLEVPAGFEHWILKFDGVQDQSREIGEPRGYGV
                     IEFAYAQMARAAGIEMTDCHLLEDAGRRHFMTRRFDRTESGRRLHMQSLAALGHLDFN
                     LPGANSYEQAFRMIDRLGLGRAAVEQQFLRMVFNVVARNQDDHVKNIAFLMDRGGEWA
                     LSPAFDLTWAYNPTGRWTSAHQMSVNGKRDGFEPGDFFEVGKLVSMKRGRVEAMLDKV
                     TESVSAWPELAADAGVEESVIKRIADTHRLDLGQSK"
BASE COUNT          294 a          636 c          596 g          284 t
ORIGIN      
        1 aaacgccagg cgaagttgaa gccgatcttg agatggccga agccgagccg gttcgagtaa
       61 cgctgcgcgg tcttcacccc ggcgaccagc gcccgggtcg agttcagcca ggcgccgtcc
      121 gatgtgttcg gggagacggt cagattgacc tcgttggtga tctgcagggc ccggaccgcc
      181 ttgatcggac cgaaggcgcg gaccacgtcc ctcacgtagt cgagccaggc tccgatgtca
      241 ccgttctgct gcggccgggg gtggtacctg acctggagct cgaccccgaa tcccagccgg
      301 ccgtagtgcc gggccatctt ctggaactgc cttatgccac gccagccgtc ggagaggaag
      361 agccggttga gccggaccgt gaaatcgcga tcgccggcca gacgcctcag ggcacggtcg
      421 cgctcctgcc agttctcggg caccagcggc gtggtctggc cgctgccgat ctctccggcc
      481 agccgggggc cgaccccgaa atggatccgg ggaccgggaa ccggctcgtc gtcggcgtag
      541 ctctgctggc aaagcgggtc gggcagaacc ggggccgtcg tctcctccgg gggaacggcc
      601 ccgcccaggt cccagggaat cgcgcccgaa ggagcggccc agaccatcaa gccggccagc
      661 accgtcgcca gcacgcatcc gaagcgcccc ggcgagctca cttgctctgc cccagatcga
      721 ggcgatgggt gtcagcgatc cgcttgatca cgctttcttc aacaccggca tcggccgcaa
      781 gctccggcca tgccgaaacc gattcggtca ccttatccag catcgcctcc acccgacccc
      841 gcttcatcga gaccagtttc cccacctcga agaagtctcc gggctcgaaa ccgtcgcgct
      901 tgccgttgac cgacatctgg tgggcggagg tccagcggcc ggtcgggttg taggcccagg
      961 tcaggtcgaa ggccggagaa agagcccact cgccaccgcg atccatgagg aaggcgatgt
     1021 tcttcacgtg gtcgtcctga tttcgagcca cgacgttgaa gaccatgcgc aggaactgct
     1081 gctcgaccgc cgcccggccc aggccgaggc ggtcgatcat gcggaaggcc tgctcgtagg
     1141 aatttgcccc cggcagattg aagtcgaggt gcccgagggc ggccagcgac tgcatgtgca
     1201 ggcggcggcc ggactcggtg cggtcgaagc ggcgggtcat gaagtggcgc cggccagcat
     1261 cttcgagcag gtggcagtcg gtcatctcga tccctgccgc cctcgccatc tgtgcgtagg
     1321 cgaactcgat cactccgtaa ccgcgcggct cgccgatctc gcggctttgg tcctggacac
     1381 cgtcgaactt gaggatccag tgctcgaacc cggccggcac ctcgagctga ccggagcgga
     1441 cctccatcgt ctccgggttg aaggcgagca gggccttggc ccgggccccg ccggccgagg
     1501 tgccgacccg caggatctcg ccgagcgccc gttcccggtc ctcgcttgcg aagctttccc
     1561 tgatcccgga ccgctcggca aggatctccg ccgccagctc ggccagggcc tcgacctcga
     1621 ccggatggct ctcgaccggc tctggaccgg tcgccggcag gtactcgagc gcccccattc
     1681 cccggcggcc cgtgtaacag agccgctcga ccgctcccat gctgccgggc tcgcgaccct
     1741 ggcgggccag ccaggcgtcg atcagggcat tgccgaagcg atccggcagc gaatccgaaa
     1801 gcatgccggg
//
LOCUS       DAOQFI010000458         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_41639_length_1810_cov_3.365812, whole genome shotgun sequence.
ACCESSION   DAOQFI010000458 DAOQFI010000000
VERSION     DAOQFI010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41639_length_1810_cov_3.365812"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /gene="hisG"
                     /locus_tag="PLE93_08100"
     CDS             complement(<1..640)
                     /gene="hisG"
                     /locus_tag="PLE93_08100"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNH87049.1"
                     /translation="MFDELRKPGGKLKLAVPRGALLDEVLDLLEKVGIETAAVRGDSR
                     SLVFDGGELTVITMRPSDVPTYVSAGAADLGITGKDVLLEQGDRPVYELVDLGIGPCR
                     MVLATQKGDVAPSEHQRRLGMMKIATKYKRIAEEYFAEQGRQVELVEVKGSIELAPLV
                     GLADGIVDLVATGRTLADNNLEVREEILSSTGRLVANRVSYRIRREEVDGLAQ"
     gene            complement(633..1652)
                     /gene="hisZ"
                     /locus_tag="PLE93_08105"
     CDS             complement(633..1652)
                     /gene="hisZ"
                     /locus_tag="PLE93_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNH87050.1"
                     /translation="MIRPIPPGTRDVLPDEMRELRAIQSALLGTFDAAGFSEVRTPTL
                     EYADALELGDGRSADSAYRFFDELGEMLALRTDMTIPIARLVADRFRDLDPPHRLCYF
                     ANAYRAVTPQRGELREFGQAGVELIGVPGPEGTAEVVELLDRSLTAVGLDEAVIGLGE
                     DGLWTALLQDAGAGPETIELSSELLVSHDLVGVERALAESDELDPEAIGQAATLIQLR
                     GGAEVLEAARGLGGQGFAVVLERLEENFRAITDRVSSRSVLLDLGMLREIGYYSGSTI
                     EVYDETVGDVIGGGGRYDDLMNRFGLDLPAAGFTLYTERIHKAQLERRGVGPRAATGE
                     RDSDV"
BASE COUNT          288 a          640 c          569 g          313 t
ORIGIN      
        1 gctgcgccag cccgtccact tcctcgcggc ggatccggta cgagacccgg ttcgcgacca
       61 ggcggccggt gctgctcagg atctcctccc ggacctcgag gttgttgtcg gccagggtgc
      121 gaccggtcgc gaccaggtcg acgatgccgt cggccaggcc gaccagcgga gcgagttcga
      181 tcgaaccctt gacttcgacc agttcaacct gccgtccctg ctcggcgaag tactcctcgg
      241 caatccgctt gtacttggta gcgatcttca tcatgcccag acggcgctga tgctcggaag
      301 gggcaacgtc acccttctgg gtcgccagaa ccatgcggca gggtccgatc ccgagatcga
      361 ccagttcgta gacgggccgg tcaccctgtt cgagcagcac gtccttgccg gtgatgccca
      421 ggtcggcggc gccggcggaa acgtaggtgg gcacgtccga cgggcgcatc gtgatcacag
      481 tcagctcgcc tccgtcgaag accagcgagc gggaatcgcc gcgcacggcg gcggtctcga
      541 tcccgacctt ctcgagcagg tcgagcactt cgtccagcaa cgcgcctctg ggcaccgcca
      601 gcttgagctt tccgccgggc ttgcgaagct cgtcaaacat cactgtcccg ttccccggtt
      661 gccgctcgcg ggccgacgcc ccttcgctcc agctgggcct tgtggatccg ctccgtatag
      721 agggtgaatc cggccgccgg caaatcgaga ccgaaccggt tcatcaggtc gtcgtagcgg
      781 ccgccgcctc cgatcacgtc accgaccgtc tcgtcataaa cctcgatcgt cgagccgctg
      841 tagtagccga tctcgcgcag catgcccaga tcgagcagca cgctgcgact gctgacccgg
      901 tccgtgatcg ccctgaagtt ttcttccagg cgttccagca caacggcaaa accctgaccg
      961 ccgagaccgc gggccgcttc caggacttcg gctccgcccc gaagctggat cagggtggcc
     1021 gcctgtccga ttgcttccgg gtccagttcg tcggattcgg ccagggcccg ttcgacaccg
     1081 accaggtcgt gagagaccag cagctcggag ctgagttcga tcgtctccgg ccccgccccg
     1141 gcgtcctgca gcagcgcggt ccagagtccg tcctcgccga gaccgatcac ggcctcgtcc
     1201 agtcctactg ccgtcagcga acggtcgagc aactcgacca cctcggccgt gccctccggg
     1261 cccggcacac cgatcagttc caccccggcc tggccgaact cacgcagctc gccccgctgc
     1321 ggggtgaccg cgcggtaggc gttggcgaag tagcagaggc gatgcggcgg gtcgagatcg
     1381 cggaagcggt cagcgaccag gcgggcgatc gggatcgtca tgtccgtcct gagcgccagc
     1441 atctcgccca gttcgtcgaa gaagcggtag gccgaatcgg ccgaacggcc gtcgccgagc
     1501 tccagggcat ccgcatattc gagggtcggg gtccggacct cgctgaatcc cgccgcgtca
     1561 aaggttccaa gcagggccga ctggatggcg cgaagttcgc gcatctcgtc cgggagcacg
     1621 tcccgggttc ctggggggat gggacggatc aaccttgtcc ttccctactc gtctacacgc
     1681 tcgatatggg cgccgagcga acgcagccgc tcgtcgatgt tctcgtaacc gcggtcgatc
     1741 tggcggatgt ttccgatctc cgacttgcct tccgcggcga gcgccgcgat cagcatcgcc
     1801 atcccggccc
//
LOCUS       DAOQFI010000459         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_41736_length_1808_cov_3.568169, whole genome shotgun sequence.
ACCESSION   DAOQFI010000459 DAOQFI010000000
VERSION     DAOQFI010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41736_length_1808_cov_3.568169"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PLE93_08110"
     CDS             complement(<1..1002)
                     /locus_tag="PLE93_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH87051.1"
                     /translation="MELIRNFSIIAHIDHGKSTLADRILELTKAVDPLKMQAQVLDSM
                     DLERERGITIKAQAVRVEFMASDGVTYHLHLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAAQGVEAQTVANTYLAIENGLELIPVLNKVDLPGAEPERVAAEVSDLIGVDPDDILR
                     ISAKTGEGVIEVLEAVVDRIPPPEGDPDAPARALIFDSEFDQYRGVIAYVRLMDGRFR
                     KHGKVLAMQNGTEAELDDIGFFRPVMTPVDGIDTGEVGYVITGIKDVSKLRVGDTLTN
                     LENPAAEALAGYREVKPMVFCGLFPIDTDRYEDLRDALDKMGLNDAALSWEPETSEAL
                     "
     gene            complement(1031..>1808)
                     /locus_tag="PLE93_08115"
     CDS             complement(1031..>1808)
                     /locus_tag="PLE93_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87052.1"
                     /translation="GPAGEPNGFVSGLRYLAPALAVCLALLGSGVGQRGGRARWLVAG
                     LLLILAPVTIAFGDGWDAKEWLVAIVAATVFWVVCLIALGVWRVRSKQHRLSTRSSRK
                     LFGWTAGATIAFLVLCGFGVQRYYFNHRYASPDFTIPALSESYVWANGQSGVKIGTNA
                     TRQYPLWGEDLDNEVQFIGVKQPHAGFVEAGNCRDFIAAANQGDYEYLVLTLDREGQK
                     LLVPRELTWVSRDPAVKNVIDADGGAVLALDGPLSPRSCR"
BASE COUNT          310 a          569 c          626 g          303 t
ORIGIN      
        1 cagcgcctcg gaagtctcgg gctcccagga aagggcggcg tcgttcaggc ccatcttgtc
       61 gagtgcgtcc cgcaggtcct cgtagcggtc ggtgtcgatc gggaaaaggc cgcagaagac
      121 catcggcttg acctcgcggt agccggccag cgcctcggct gcggggttct cgaggttggt
      181 cagcgtgtca ccgacccgca gcttcgaaac gtccttgatg ccggtgatca cgtagccgac
      241 ctcgccggtg tcgatcccgt cgaccggggt catgaccggg cgaaagaagc cgatgtcatc
      301 cagttcggcc tcggtgccgt tctgcatcgc cagcaccttc ccgtgcttgc ggaagcgccc
      361 gtccatcaac cgcacgtagg cgatcacgcc gcggtactgg tcgaattcgg aatcgaagat
      421 cagggcccgg gccggggcat cggggtcgcc ttccggcggc gggatgcggt cgaccaccgc
      481 ttcgagcact tcgatcacgc cctcgccggt cttggcggag atgcgaagga tgtcgtcggg
      541 atcgacgccg atcaggtccg agacttctgc cgcgacccgt tccggctcgg cgccgggcag
      601 gtcaaccttg ttcaggacgg gaatgagctc gagcccgttc tcgatcgcca ggtaggtgtt
      661 ggcgacggtc tgggcctcga ccccctgggc agcatcgacg accaggagcg caccctcgca
      721 ggcggcgagg ctgcgcgaga cctcgtatga gaagtcgacg tgaccgggag tgtcgatcag
      781 gtgcaggtgg taggtgacgc cgtcggaggc catgaactcg acccgcaccg cctgggcctt
      841 gatggttatt ccgcgctcac gctcgagatc catcgagtcc agaacctggg cctgcatttt
      901 cagcgggtcc acggccttgg tcagttcgag gatgcggtcg gccagggtcg acttgccatg
      961 gtcgatgtgg gcgatgatcg agaagttgcg aatcagttcc atcagggaca gagcagtatg
     1021 ccgcagggga ctatcggcag gatcgcggcg agagtggccc gtcgagggcg agtaccgccc
     1081 ctccgtcggc gtcgatcacg tttttgaccg cgggatcccg agaaacccag gtcagctccc
     1141 ggggcacgag cagcttctgg ccttcgcggt cgagggtgag gaccaggtac tcgtaatcgc
     1201 cctggttggc ggcggcgatg aagtcgcgac agttcccggc ttcgacgaag ccggcgtggg
     1261 gctgcttgac gccgatgaac tggacttcgt tgtcgaggtc ctcgccccaa agcgggtact
     1321 ggcgggttgc gttggtgccg atcttcactc cgctctggcc gttcgcccag acatatgact
     1381 cggacagggc cgggatcgtg aaatcgggcg aggcgtagcg gtggttgaag taatagcgct
     1441 ggaccccgaa cccgcagagg acgaggaatg cgatcgtcgc gccggcggtc cagccgaaaa
     1501 gtttccggga agagcgggtc gagagtctgt gctgtttcga ccggacccgc cagaccccga
     1561 gggcgatcag acagacaacc cagaagaccg tcgccgccac gatcgcaacc agccattcct
     1621 tggcgtccca gccatcaccg aaggcgatcg tcaccggggc caggatgagc agcagaccgg
     1681 ccacgagcca gcgggcccgg ccgccgcgct gaccgacgcc gctgccgagc agcgctaggc
     1741 agaccgccag cgccggggcc aggtaccgga gtccggagac gaatccgttc ggttccccgg
     1801 cggggccg
//
LOCUS       DAOQFI010000460         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42356_length_1794_cov_4.052329, whole genome shotgun sequence.
ACCESSION   DAOQFI010000460 DAOQFI010000000
VERSION     DAOQFI010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42356_length_1794_cov_4.052329"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLE93_08120"
     CDS             <1..182
                     /locus_tag="PLE93_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HNH87053.1"
                     /translation="LIETYIIDEEVDLYGRILRVAFVERLRGEKRFDSVDDLIAQMKR
                     DVEKTKAVCASFHRP"
     gene            224..493
                     /gene="rpsO"
                     /locus_tag="PLE93_08125"
     CDS             224..493
                     /gene="rpsO"
                     /locus_tag="PLE93_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNH87054.1"
                     /translation="MTLTKENKAELISKYGKSEGDTGSTEVQVALLTERINDLTGHLR
                     EHGKDHHSRRGLLMMVGKRRRLLRYLERTDVERYRALVADLGLRH"
     gene            490..942
                     /locus_tag="PLE93_08130"
     CDS             490..942
                     /locus_tag="PLE93_08130"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNH87055.1"
                     /translation="MIEVGAKAPDFTLPDHAGQEVSLSDFSGRRVLLAFYPLDFSPVC
                     SDQLAQYKAIQGELEEAGVTLLGVSVDSAFAHKAFRDQLGIETSLLADFEPKGAVAEA
                     YGAYLGQAGHSNRSLVLIDADGVVEWVHETATPLDIPGPDLIVEAIRA"
     gene            948..1397
                     /locus_tag="PLE93_08135"
     CDS             948..1397
                     /locus_tag="PLE93_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87056.1"
                     /translation="MKPWIASALVLAALAAGCGGGSDDSPSPAPPAGEIKVIAESDAD
                     QKIANDLAKYLDRNCPASVRQSDNPTLQQLYTATSEFCGGVTAIEVRDKRITVQSDLE
                     PGAGSETLGNVFCVYVAGSDVADFTPGHTLLDASGKKIKTCEARPLP"
     gene            complement(1418..>1794)
                     /locus_tag="PLE93_08140"
     CDS             complement(1418..>1794)
                     /locus_tag="PLE93_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87057.1"
                     /translation="GALAAAAVSLVLVLGGGDGEGTGNSGQQFAFGSLPAGVTITGNL
                     EPKATGTGIEVRVDGMKPGTLCRVYVKTSAGTLEPAGTFRYRYDTDEHESYPATLSTA
                     WDLSEIDSLVIRAGPETFETGL"
BASE COUNT          326 a          609 c          552 g          307 t
ORIGIN      
        1 tcctgatcga gacctacatc atcgacgagg aggtcgatct gtatggccgg atcctccggg
       61 tcgccttcgt cgaacggttg cgcggcgaga aaaggttcga ctcggtcgac gatctgatcg
      121 cgcagatgaa acgggacgta gagaagacga aggccgtctg tgctagcttc catcggccgt
      181 gaacgggtcc gcccgtgacc cgaaaccaag gatttttgaa gaaatgacac tgaccaaaga
      241 aaacaaggcc gaactgatca gcaagtacgg caagtccgag ggcgacaccg gctcgaccga
      301 agtccaggtg gccctgctta ccgagcggat caacgatctc accggccacc tgcgggagca
      361 cgggaaggat caccattccc gccgcgggct gctgatgatg gtcggcaagc gccgccgcct
      421 gctgcgctac ctcgagcgga ctgacgtcga gcgctaccgc gccctcgtcg ccgacctcgg
      481 cctgcgccat tgatcgaagt cggggccaag gctccggatt tcaccctgcc tgaccacgcc
      541 ggtcaggaag tttccctgtc ggacttttcc ggccgccggg tgctgctcgc cttctacccg
      601 ctggacttca gcccggtctg ctccgatcag ctggcccagt acaaggcgat tcagggcgaa
      661 ctcgaagagg ccggcgtcac cctgctcgga gtcagcgtcg attcggcctt cgctcacaag
      721 gcgttccggg accagctcgg gatcgaaacc tcgctgctcg ctgatttcga gcccaagggc
      781 gcggtcgccg aagcctacgg cgcctacctc ggccaggccg gccattcgaa ccgttccctg
      841 gtcctgattg acgccgacgg cgttgtcgag tgggtccacg agacagcgac gccgctcgac
      901 atcccgggtc ccgacctgat tgtcgaagcc atccgggcct agccgccatg aagccctgga
      961 tcgcttccgc gctggtgctt gcggcgctcg ccgccggatg cgggggagga agcgacgatt
     1021 caccatcccc ggccccgccg gccggggaaa tcaaggtcat cgccgaatcg gacgccgatc
     1081 agaagatcgc caatgacctc gccaaatacc tagaccggaa ctgcccggcc agcgttcgcc
     1141 aaagcgacaa tcccacgctg cagcagctct acacggctac ttccgagttc tgtggcggcg
     1201 tcaccgcgat cgaggtcagg gacaagagga tcacggttca aagcgacctc gagccgggcg
     1261 ccggctcgga gacgctcggc aacgtcttct gtgtatacgt cgcaggttcc gatgtcgccg
     1321 atttcacacc gggccatacc ctgctcgacg cttccggcaa aaagatcaag acctgtgagg
     1381 cccgcccgct gccctgaccg gccggtgccc gcgcggccta gagcccggtt tcgaaggtct
     1441 ccggtccggc cctgatcacg aggctgtcga tttcactcag atcccaggcc gttgagaggg
     1501 tggccgggta ggactcgtgt tcatccgtgt cgtagcggta acggaaggtt ccggctggtt
     1561 ccagcgtgcc ggccgaggtc ttcacgtaaa cccggcacag cgtgccgggc ttcatcccgt
     1621 cgaccctgac ttcgatcccg gtcccggttg ccttcggttc gagattgccg gtaatcgtca
     1681 cgccggccgg gagggagccg aaagcgaact gctgaccgct attcccggtt ccctcgccat
     1741 cgccgccgcc cagcaccaga accaggctga ccgcggccgc cgccagcgcc ccgg
//
LOCUS       DAOQFI010000461         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42481_length_1791_cov_3.161290, whole genome shotgun sequence.
ACCESSION   DAOQFI010000461 DAOQFI010000000
VERSION     DAOQFI010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42481_length_1791_cov_3.161290"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1791)
                     /locus_tag="PLE93_08145"
     CDS             complement(<1..>1791)
                     /locus_tag="PLE93_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNH87058.1"
                     /translation="VVVVTGDRDAYQLVTDTVQVMSTSRGVTETKLYDREAVKERYGV
                     YPELVTDLMGLRGDTSDNIPGVPGIGEKTAAQLLDQFGSLEAILAGVDEVSGKKRKEN
                     LIEFAEDARISKQLATMDFEVETGLDVDELMAQTPDREGLRAFMTEFELRAAMKRLEE
                     ALEDEEIPGVSEEAPAPAEVEVSKGGVGDLDEGPIAVELYGGWAATDGRKVVGGEATA
                     EQLAGMVQGKPLIAHDIKAVGGQRVGLLAALEATEQGPVISHDTLVAAYLLEPSRRNY
                     HLDELAVQAGLAPSGIDGDDAQMSLIADEQDSGSIAPEIAATVWNLARVQRPKLEEAG
                     LLDLLEQVEQPLIRVLAAMERIGLKLDRERVAEMAAGLEDRIIELETEIHGLAGHEFT
                     IGSPQQVGQVLFEELGLTRKRKGKTGFSTDARVLAQIRQEHAIVALIEQWRELTKLKN
                     TYLEPLPAAIDPATGRVHTTFMQVAAPTGRISSIQPNLQSIPIRSEVGRPLRGCFVAE
                     PGNLLTSLDYSQVELRVLAHVADEEVLKGFFRAGEDVHSATAAQVFEIPPEEVDEAQR
                     SKAKMVNFGIVYGLTGFGLADRLNIPRSVEG"
BASE COUNT          265 a          616 c          569 g          341 t
ORIGIN      
        1 ccttcgacgg agcgggggat gttcaggcgg tcggccaggc cgaagccggt caggccgtag
       61 acgatgccga agttgaccat ctttgccttc gaccgctgcg cctcgtcgac ctcttccggg
      121 ggaatctcga agacctgggc cgcggtcgcc gagtggacat cctccccggc ccggaagaat
      181 cccttcagca cttcctcgtc ggcgacatgg gccagcaccc tgagctccac ctggctgtag
      241 tcgagcgagg tgagcaggtt gcccggctcg gcgacgaagc agccccggag cgggcggccg
      301 acctcggaac ggatcgggat gctctgcaga ttgggctgga tcgaggagat ccgcccggtc
      361 ggggcggcga cctgcatgaa ggtcgtgtga acgcgcccgg tcgccgggtc gattgccgcc
      421 ggcagcggct ccaggtaggt gttcttgagc ttggtcagct cgcgccactg ctcgatcagg
      481 gcgacgatcg cgtgttcctg ccggatctgg gccagcaccc ttgcgtcggt cgagaaaccg
      541 gtcttgccct tccgcttgcg ggtcaggccc agctcctcga agagcacctg gcccacctgc
      601 tgcggcgagc cgatcgtgaa ctcgtggccg gccagtccgt ggatctcggt ttccagctcg
      661 attatccggt cctcgagacc ggcggccatt tcggcgaccc gttcacgatc gagtttcagc
      721 ccgatccgct ccatggccgc gaggacccgg atcaagggct gttccacctg ctccagcaaa
      781 tcgagcaggc cggcctcctc gagcttcggc ctctggaccc gggccaggtt ccacaccgtg
      841 gcggcgatct ccggggcgat cgatccggag tcctgttcgt cggcgatcag cgacatctgg
      901 gcgtcgtcac cgtcgatgcc ggaaggtgcc agaccggcct gcaccgcgag ttcgtcgagg
      961 tggtagttcc gcctcgaggg ttcgagcaga taggcggcga ccagtgtgtc gtgactgatc
     1021 accggcccct gttcggtcgc ttcgagcgcc gccagcaacc caacccgctg gcccccgact
     1081 gccttgatgt catgcgctat caacggcttc ccctggacca tgccggccag ttgttcggcg
     1141 gtcgcctcgc cgccaacgac tttccggccg tcggtcgccg cccatccgcc gtagagctcg
     1201 accgcgatcg gcccctcgtc gaggtctccg acaccgcctt tcgagacttc gacttcggcc
     1261 ggggccggag cttcctcgga aacgccgggg atctcttcgt cctccagggc ttcttcgagc
     1321 cgtttcatcg ccgccctgag ctcaaactcg gtcatgaagg cgcgaaggcc ctcccggtcc
     1381 ggggtctggg ccatcagctc atcgacgtcc agccccgttt caacttcgaa atccatcgtc
     1441 gcaagctgct tggagatgcg ggcatcctcg gcgaactcga tcaggttctc cttgcgcttc
     1501 ttgcccgaga cctcgtcgac gccggcgaga atcgcttcca gtgagccgaa ctggtcgagc
     1561 agctgagcgg cggtcttctc gccgatcccc gggactcccg gaatgttgtc ggaggtgtca
     1621 ccgcgaagcc ccatcaggtc ggtgaccagt tccgggtaga cgccgtatcg ctccttgacc
     1681 gcctcgcggt cgtagagctt ggtttcggtc acgcctctgg aggtgctcat gacctgaacc
     1741 gtgtcggtca ccagctggta ggcgtcacgg tcaccggtga ccacgaccac c
//
LOCUS       DAOQFI010000462         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42543_length_1790_cov_2.706628, whole genome shotgun sequence.
ACCESSION   DAOQFI010000462 DAOQFI010000000
VERSION     DAOQFI010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42543_length_1790_cov_2.706628"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLE93_08150"
     CDS             complement(<1..359)
                     /locus_tag="PLE93_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87059.1"
                     /translation="MLAFALRRLGSMVLVLFAVSVLTFLIFNVLPSGDPAVRMAGKQP
                     TETQIEAIREEWGFNEPVYVQYVKTMENLFSGNMISYFNAGTQWAVAKGNPENIAPDN
                     ACGKKIGVQKATVQVDD"
     gene            complement(359..1399)
                     /locus_tag="PLE93_08155"
     CDS             complement(359..1399)
                     /locus_tag="PLE93_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH87060.1"
                     /translation="MSGATDRPVSDLQEFSEPPAVTQEGVAGESPWRLAVRRMRKRRF
                     ALFMGAVFVLLVLTMAAAPLWAEHVAHTTPSANHLSDQIEVDGEKVNVVGLDGVPIGP
                     TWQGQFFLGADTNGRDVAVRLLYGGRNSLIIGLGAALITLVLGLVTGLLAGYFRGWTD
                     SILSRIMDVLWAFPVILLGIALGTSLALGGLKLGPVSVSGGSLAIPIFVIAIVYIPYL
                     ARPIRGEVLSLREKEFVEAARAQGMGSFRLITSEILPNVLSTGLVFFPLLVANAILLE
                     AALSFLGAGVQPPEPSWGTMIDEGVARIVTAPALAIVPGMMIVLTVLSLNVFGEAIRD
                     SLDPKAKVRIEH"
     gene            complement(1438..>1790)
                     /locus_tag="PLE93_08160"
     CDS             complement(1438..>1790)
                     /locus_tag="PLE93_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87061.1"
                     /translation="KAQTGFANWFQDFPHPLNFSFLVDGDSIQDTNNQNFGNVDDPSL
                     NKTINELTLETDLPSVADRWEAWDQELVSPPNSYVAPYGHRKLATFVSDRIDFDAIVF
                     HPLYNNDYSTFKLK"
BASE COUNT          332 a          553 c          595 g          310 t
ORIGIN      
        1 atgtcgtcga cctgcaccgt ggccttctga acgccgatct tcttgccgca ggcgttgtcg
       61 ggagcgatgt tctccggatt gcccttggcg accgcccact gggttcccgc gttgaagtag
      121 ctgatcatgt tgccgctgaa caggttttcc atggtcttca cgtactggac gtagaccggt
      181 tcgttgaagc cccactcctc cctgatcgcc tcgatctggg tttcggtcgg ctgcttgccg
      241 gccatgcgga ccgcggggtc gccgctgggc aggacgttga agatcaggaa ggtcaggacg
      301 gacaccgcga acagcaccag gaccatcgag cccagccgcc tcagagcgaa cgccagcatc
      361 agtgttcgat cctcactttc gccttcgggt caagcgagtc gcggatcgct tcgccgaaga
      421 cgttgagcga aagcacggtg agcacgatca tcatgccggg cacgatcgcc agggcgggcg
      481 cggtgacgat tcgcgcgaca ccctcgtcga tcatggtccc ccaggagggc tcgggtggct
      541 ggacaccggc tccgagaaag gagagcgcgg cttcgagcag gatcgcgttg gcgaccagga
      601 gcggaaagaa gacgagcccg gtcgaaagca cgttgggcag gatttcggag gtgatcaggc
      661 ggaaggagcc catgccctgg gcccgggccg cctcgacgaa ctccttctcc cgcaggctca
      721 gcacctcgcc ccggatcggc cgggccaggt aggggatgta gacgatcgcg atcacgaaga
      781 tcggaatcgc gaggctgccg cccgacaccg acaccggacc gagcttgagc cctccgagag
      841 ccagcgaggt gcccagggcg atccccagca ggatcaccgg gaaagcccag aggacgtcca
      901 tgatcctcga aaggatcgaa tcggtccagc cccggaaata tccggccagg aggccggtca
      961 ccagaccgag gaccagggtg atcagggccg ctccgaggcc gatgatcagc gagttccggc
     1021 caccgtagag cagccgtacg gccacgtcgc gcccgttggt gtcggcgccg aggaagaact
     1081 ggccttgcca ggtcggcccg atcggcaccc cgtccagccc gaccacgttc accttctcgc
     1141 catcgacttc gatctggtcg gagagatggt tcgcggaggg cgtggtgtga gccacgtgct
     1201 cggcccagag cggagcggcc gccatggtca ggacgagcag gacgaagacc gctcccatga
     1261 agagagcgaa ccgccgtttc cgcatccgcc tgaccgccag ccgccacgga ctctcgccgg
     1321 ccaccccctc ctgggtgacg gccggcggtt ccgagaattc ctggaggtca gatacgggcc
     1381 tgtccgtggc gccgctcact cttggtcaat gactgctttc gagggcctcg ggaatagcta
     1441 cttgagcttg aacgtggagt agtcgttgtt gtagagcgga tggaagacga tcgcgtcgaa
     1501 atcgatccgg tcggaaacga aggtcgccag tttgcgatgt ccgtacggag cgacatagct
     1561 gttcggcggc gagacgagct cctggtccca ggcctcccag cggtcggcga ccgagggcag
     1621 gtcggtttcc agggtcagct cgttgatcgt cttgttcagg ctcggatcat cgacgttgcc
     1681 gaagttctgg ttgttggtgt cctgaatcga atccccgtcc accaggaagg agaagttgag
     1741 cggatgcggg aaatcctgga accagttggc gaagcccgtc tgggctttcg
//
LOCUS       DAOQFI010000463         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42625_length_1788_cov_3.971725, whole genome shotgun sequence.
ACCESSION   DAOQFI010000463 DAOQFI010000000
VERSION     DAOQFI010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42625_length_1788_cov_3.971725"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLE93_08165"
     CDS             complement(<1..584)
                     /locus_tag="PLE93_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH87062.1"
                     /translation="MAGTARREGDLRNYVLVTAAYWADTLADGASRILVLFFFYQLGY
                     SALEVATLFLFYEFFGIVTNLVGGWIASRLGLKATLFSGLTIQIVALGMLGFAPDEWL
                     VVPYVMVSQALSGIAKDLTKMSSKSAVKLVVPEDSESSLFKWVAILTGSKNALKGVGF
                     FLGGLLLTLIGFQASMLVLLGIVATALITTASMMR"
     gene            complement(588..1613)
                     /locus_tag="PLE93_08170"
     CDS             complement(588..1613)
                     /locus_tag="PLE93_08170"
                     /inference="COORDINATES: protein motif:HMM:NF033735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNH87063.1"
                     /translation="MTDKLRIGINGFGRMGRLALRAGWDTEELEFVQINEIAGGPESA
                     AHLLEFDSVQGRWRREGIEWLEDSIRVAGKELAYTSGSDPGGVDWGKDEVDLVIEATG
                     KFRTPELLAPYFGRGVKRVLVAAPVKDDALNIVYGVNDSDYDPDRDRIVTAASCTTNC
                     LAPIVKVVHEGIGIRHGSITTLHDLTNTQVVVDAHHKDLRRARSAGTSLIPTSTGSAT
                     AIGMIFPDLVGRLDGLAVRVPLLNSSITDCCFEVDRATSVEEINDLLRTAASEELGGI
                     LGFETRPLVSVDFKGDPRSGIVDGTSTRVIDQTQVKILAFYDNEWGYANRMIDIARMI
                     ARSSGGD"
BASE COUNT          338 a          554 c          555 g          341 t
ORIGIN      
        1 cgcatcatcg acgcggtggt aatcagggcg gtggcgacga tcccgagcaa taccagcatc
       61 gaggcctgga acccgatcag ggtgagcagc agcccgccga ggaagaagcc gacgcccttg
      121 agggcattct tggaaccggt gaggatcgcc acccatttga agagagatga ttcggagtcc
      181 tctgggacga cgagcttgac cgcactcttc gagctcatct tggtcaggtc cttggcgatg
      241 ccggagagcg cctgggagac catcacgtac ggaacgacca gccactcgtc gggcgcgaaa
      301 ccgagcatcc cgagcgcgac gatctggatg gtcagcccgg agaaaagcgt cgccttcaat
      361 ccgagccggg aggcaatcca tcctccgacc aggttggtga cgatcccgaa gaactcgtag
      421 aagaggaaca gcgtcgcgac ttccagcgcg ctgtaaccga gctggtagaa gaagaacagg
      481 acgaggatgc ggctggcacc gtcggcgagc gtgtcggccc agtaggcggc cgtgaccagg
      541 acgtagttcc tgagatcacc ttctcttcgc gcggtgccgg ccatcgctca gtctccgccg
      601 gagcttcggg cgatcatcct ggcgatgtcg atcatccggt tcgcgtatcc ccactcgttg
      661 tcgtagaagg cgaggatctt gacctgggtc tgatcgatca cccgggtcga ggttccgtcc
      721 acgatgccgg accgggggtc acccttgaag tcgacggaaa ccagtggcct cgtctcgaag
      781 ccgaggattc ccccaagctc ctcgctggcg gccgtccgga gcaggtcatt gatttcttca
      841 acggaagttg cacggtcgac ctcgaagcag cagtcggtga tcgaggagtt gaggagcggc
      901 actcgcaccg ccagcccgtc cagtcgcccg acgagatcgg ggaagatcat tccgatcgcg
      961 gttgccgatc cggttgaggt cgggatcagc gaagtaccgg ccgacctcgc gcgccggagg
     1021 tccttgtggt gggcgtcgac cacgacctgc gtgttggtca ggtcgtggag ggtggtaatc
     1081 gatccgtgcc ggatgccgat gccctcgtgg acaaccttga cgatcggggc caggcagttc
     1141 gtcgtgcagg aggcggcggt gacgatgcga tccctgtcgg ggtcgtaatc ggagtcattc
     1201 acgccgtaga cgatgttcag cgcgtcatct ttgaccggtg ccgcgacaag gactctcttc
     1261 acgccccggc cgaagtaggg agccaggagt tccggtgtgc ggaactttcc ggtggcctcg
     1321 atcaccagat caacttcgtc cttgccccag tcaactcccc cgggatcgct ccccgaggtg
     1381 taggcaagtt ccttgccggc gacccggatc gagtcttcca gccactcgat tccttcccgg
     1441 cgccagcgtc cctgcaccga gtcgaactca agcaggtgcg ccgcagactc ggggcccccg
     1501 gcgatctcgt tgatctggac gaattcgagt tcctcggtgt cccagccggc tcgcagagcg
     1561 agcctcccca tccttccgaa gccgttgatt ccaattctca gcttgtctgt catccgtcgc
     1621 cctcccgggc ctcgtttgat ctatcgatga ctatcgtatc tattgacaaa cgtcaattga
     1681 actgatttgg tgaatatcga tgagctccgc actgaccctc agccccaaac agaagagtcc
     1741 gaacggccag ccttgttgcg agccggtcgt ctacccggac gtcgaacg
//
LOCUS       DAOQFI010000464         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42628_length_1788_cov_3.690133, whole genome shotgun sequence.
ACCESSION   DAOQFI010000464 DAOQFI010000000
VERSION     DAOQFI010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42628_length_1788_cov_3.690133"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="PLE93_08175"
     CDS             complement(<1..1250)
                     /locus_tag="PLE93_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNH87064.1"
                     /translation="MAKDTEKLIRQLSLISFLMAQGRPVSALEIKREVEGYSVMNEDA
                     FARRFYADRAELDSLGIELQVDRPGEGFFEAELYSLPPENFYLPEIKFTDDELAALRT
                     SLSLLTEGEFAYAEPLRLALQQVAWGHPNPLADPDNIPVSMAKFSATASRDLTQRLAR
                     IETAISRHKTITFEYHTIGSDKVNNRKVDPYHLVYRGGQFYLIGFAHERDAVRVFRLS
                     RIRGKVGYATKSEHDFSPPEDFERRDYAELADWQLGEEIGKARIFFSDRIAWLIERDF
                     GAHGEVSKADPEETGGRDGIVLETSYASERELLAWVLRWRQNAEVLGPPEFKAAAESR
                     IALLRDRHINGFDVAEAQTDFPNPIRERRQRSKDRSAAAIRPERFGRLVTLAGMLIGA
                     AKEGTALSMSDLCQRLELSEEELR"
     gene            complement(1363..>1788)
                     /locus_tag="PLE93_08180"
     CDS             complement(1363..>1788)
                     /locus_tag="PLE93_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87065.1"
                     /translation="VIKACDDPNLTWPEMIELGIEASLKFLAAEPAFARMCIVDMFSA
                     GPAALERYLSAVRMIASFVDAGRTMLEGREDVPESIANMVVGGAAVVIRAEIVDERTE
                     MLPEVGPDLLYAILVSYMDKEEALERSERYAERLGFVTS"
BASE COUNT          314 a          560 c          588 g          326 t
ORIGIN      
        1 tcccggagtt cctcttcgga aagctcgagg cgctggcaga ggtccgacat cgaaagggcc
       61 gtgccttcct tggcagcacc aatcagcatt cccgccaggg tgaccaggcg gccgaagcgc
      121 tccggccgga tcgccgccgc ggagcggtcc ttgctgcgct ggcgacgctc gcggatcgga
      181 ttcgggaagt cggtctgggc ctcggccacg tcgaagccgt tgatgtgacg gtcgcgcagc
      241 agggcgatcc gggattcggc ggccgccttg aactcgggcg gaccgagcac ctcggcgttc
      301 tggcgccagc ggagcaccca ggccagcagt tcccgttcgc tggcgtacga agtctcgagc
      361 acgattccgt cgcggccacc ggtttcttcc gggtcggcct tgctgacctc gccgtgcgcc
      421 ccgaagtccc gctcaatcag ccaggcgatg cggtcgctga agaagatgcg cgccttgccg
      481 atctcctcgc ccagctgcca gtcggccagt tcggcgtagt cgcgacgctc gaaatcctcg
      541 ggcgggctga agtcatgttc ggatttggtc gcgtagccga ccttgccgcg gatccgcgaa
      601 aggcggaaaa cccggaccgc atcgcgctcg tgggcgaaac cgatcaggta gaactgcccg
      661 ccgcggtaga ccaggtggta agggtcgacc ttgcggttgt tgaccttgtc ggagccgatc
      721 gtgtggtact cgaaggtgat cgtcttgtgg cgcgagatcg cggtttcgat ccgggccagg
      781 cgctgggtca gatcacgcga ggcagtggcc gagaacttgg ccatcgagac cgggatgtta
      841 tccgggtcgg ccagcggatt cggatggccc caggccacct gctgcagggc cagccgcagc
      901 ggctcggcgt aggcgaactc gccctccgtg agcaggctga gcgaggtgcg aagggccgcc
      961 agttcatcgt cggtgaactt gatctccggc agatagaaat tctccggcgg cagtgagtag
     1021 agctcggcct cgaagaagcc ttcaccgggc cggtccacct ggagttcgat gccgagactg
     1081 tcgagttcgg cccggtccgc gtagaaacgg cgtgcgaacg cgtcttcgtt catcacgctg
     1141 tagccctcga cctcccgttt gatttccagg gccgaaacgg gccggccctg agccatcaga
     1201 aacgagatca gcgagagctg ccggatcagc ttctcggtgt cctttgccat cagcgaatga
     1261 ctttacctgc gcggagtccc cggctacatc ccgaagtacc ccggatcccg ctttcaggcc
     1321 gcaaatgccg gtttttttaa cccttacgtg aggaagtcgt gactaagacg taacgaagcc
     1381 gagcctctcg gcgtaacgct cggagcgctc gagggcttct tccttgtcca tgtaggagac
     1441 aaggatggcg tagagaagat cggggccgac ctcgggcagc atctcggttc gctcgtcgac
     1501 gatttccgcc ctgatcacca ccgcagcacc gccgaccacc atgttggcga tcgattccgg
     1561 cacgtcctcg cgtccttcca gcatggtgcg gccagcgtca acgaaggagg cgatcatgcg
     1621 aaccgccgag aggtagcgct cgagggccgc ggggccggcc gagaacatgt cgacgatgca
     1681 catgcgggcg aaggcgggtt cggcagcaag gaacttgaga ctggcttcaa tgccgagttc
     1741 gatcatctcc ggccaggtca gattcgggtc atcgcaggcc ttaatcac
//
LOCUS       DAOQFI010000465         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42739_length_1786_cov_2.662623, whole genome shotgun sequence.
ACCESSION   DAOQFI010000465 DAOQFI010000000
VERSION     DAOQFI010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42739_length_1786_cov_2.662623"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PLE93_08185"
     CDS             <1..878
                     /locus_tag="PLE93_08185"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH87066.1"
                     /translation="AMDKEKYEVIPVYVAKDGAFWTGAHLADLESYRDIPAALARATE
                     VLLAREQGLDVVSVATVIGRPLTSMIWLKKSKVKSVRDLKGKTVSTAGIPYQDAYLKT
                     ILSRAGLSENDVKKVGVGQGLLPSIVSGRAQATLGPFWNIEGIELKKEGLKPTVSPVD
                     KLGVPTYSELVLVAKGSRIDEDPDPIRLFITALGRGTGFAVSHPDQATSTVLEANDAL
                     KPEVTAAQMKATLPLLGAPGGAKDAVPFGHQSTVKWQAFINWMVQQGVLANSQRAVDA
                     LTNELLPSKRIDVDQ"
     gene            complement(961..>1786)
                     /locus_tag="PLE93_08190"
     CDS             complement(961..>1786)
                     /locus_tag="PLE93_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87067.1"
                     /translation="ALLEGPGSPCLKEAGEPALEEGISRLRFSLGVDDDLAGFRNAFR
                     EDPLLGRALNGRLQYRPMRRPVAWEALLWAITEQLIEYSRAARIQRQLIRRFGVRLSP
                     GVALGADDGRNRLPRSPRGRKQPSLMTVPTPAAIAGAAPAALEACGLSPRRSIAMVKV
                     AREVASGRVDPADPAGDKRLLAIPEIGPWTIACLALRGRGEPDALLAGDLSQVKLVGY
                     LEGLGRLAEVEEVEEFYAPYAPWRGLAGEYIAMAYGSAVHGPGNRARIRQQLRPAA"
BASE COUNT          315 a          598 c          613 g          260 t
ORIGIN      
        1 ccgccatgga caaggagaaa tatgaggtga tcccggtcta tgtcgccaag gacggcgcct
       61 tctggaccgg ggcgcatctg gccgatctcg agagttaccg cgacatcccc gccgcgctgg
      121 cgcgggcgac cgaggtgctg ctggcccgcg agcagggtct cgacgtggtt tcagttgcga
      181 cggttatcgg ccgaccgctc acctcgatga tctggctgaa gaagtcgaag gtgaagagtg
      241 tcagggacct caagggcaag actgtttcaa ccgccgggat tccctaccag gacgcctacc
      301 tgaagacgat cctctcgcgg gcgggactga gcgagaacga cgtgaagaag gtcggggtcg
      361 gccagggcct tctgccgtcg atcgtcagcg gccgggccca ggcgacgctc ggccccttct
      421 ggaacatcga gggaatcgaa ctgaagaagg aaggcctcaa gccgaccgtg agcccggtcg
      481 acaagctcgg cgtcccgacc tatagcgagc tggtcctggt cgccaagggc agtcggatcg
      541 acgaagaccc ggacccgatc cgcctcttca tcacggcact cggccgggga accggttttg
      601 cggtctccca tcccgaccag gcaaccagca ccgtgctcga agccaatgac gccctgaagc
      661 ccgaggtcac ggccgcccag atgaaggcga ccctgccgct gctgggcgcg cccggaggcg
      721 cgaaggacgc ggttccgttc ggtcaccaga gcaccgtgaa gtggcaggca ttcatcaact
      781 ggatggtcca gcagggagtg ctggccaact cgcagcgcgc ggtcgacgcc ctgaccaacg
      841 agcttctgcc cagcaagcgg atagacgtgg atcagtaagt acgcattccg gtctgcgccg
      901 gaatgccaat gaaacccgtg cgtccgttca ggacatcaac gacttgatga acggactccg
      961 ttatgcggcg ggtcgcagct gctggcggat ccgggcccgg ttgcccgggc cgtgaaccgc
     1021 cgagccgtag gccatggcga tgtactcgcc ggccaggccc cgccagggtg cgtacggggc
     1081 atagaactct tcaacctctt cgacctcggc cagccggccc aggccttcga ggtaaccgac
     1141 cagtttgacc tggctgaggt cgccggccag cagcgcgtcg ggctcaccgc ggccacgcag
     1201 ggccaggcag gcgatcgtcc aggggccgat ctccgggatc gccagcaggc gcttgtcccc
     1261 ggctgggtcc gccgggtcga cccggcccga ggcgacctcg cgggcaacct tgaccatggc
     1321 gatcgaacgc cgtggactga gcccgcaggc ctcgagagcg gccggagcgg caccggcgat
     1381 cgccgcgggg gtgggcacgg tcatgaggct cggctgtttc cggccgcgag gcgagcgggg
     1441 aagccggttt cggccgtcat cggcgccgag cgccacaccg ggcgagaggc ggaccccgaa
     1501 gcggcggatc agctgccgct ggatccgggc cgcccggctg tactcgatca gttgctcggt
     1561 gattgcccaa agcagggcct cccaggcgac cggccgccgc atcggccggt actggagacg
     1621 accgttcagg gcccggccga gcagcggatc ctcgcgaaat gcgttgcgga acccggccag
     1681 gtcgtcgtca actccgaggc tgaagcgcaa cctggagatc ccctcctcga gcgccggttc
     1741 ccccgcctcc ttcaggcagg gcgagcccgg accttcgagc agagcc
//
LOCUS       DAOQFI010000466         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42774_length_1785_cov_3.287283, whole genome shotgun sequence.
ACCESSION   DAOQFI010000466 DAOQFI010000000
VERSION     DAOQFI010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42774_length_1785_cov_3.287283"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PLE93_08195"
     CDS             <1..1250
                     /locus_tag="PLE93_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNH87068.1"
                     /translation="FRYHHLFRDMLRSEMNLRDPGAKPGLHRRAAVWLAANGDIPRAT
                     DHAIAAGDFELAGACIWLDLPDLLSRGRVATIDRWFETIGDDRIAMIPGLLLARAHRE
                     IAMGRGDETFYWLSVAEREIAEDSPLFGDVLTLRATTGINGARGMIADGERAAGLMSP
                     TSPFQVAAKLYVGIGLGLTDQENASAVLRESAREAAMISGIIQAIALTELALLELDSG
                     RVDAAHDYASRARDVVERSGLRILGVMSLVYVVLALTKAERGRVEEAIVDLETSSRIG
                     EGILDYIVWHEAQLVLCRCRTLIRIGQFAQAESELGRARIAIGRVPDSPFLERWLEEC
                     EGELEMVRGSGGTTAVSLTRAELRTLQFLPSHHSFRSIGEQLYLSQNTVKTQANSLYR
                     KLGVNSRAEAVIEGRRLGLLKGE"
     gene            1335..1769
                     /locus_tag="PLE93_08200"
     CDS             1335..1769
                     /locus_tag="PLE93_08200"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HNH87069.1"
                     /translation="MTEEIEEMGPIDYILVEWPNNEPTGEAIPYLVDLVDSGLIRILD
                     LAFIMKNEDGSVTEMEISDLGGESESFTVFEGASSGILDESDHAEAAEALETGKAAAL
                     LVYENSWAGPFASAVRRSGGQLVASGRIPVQALIASLDSAEA"
BASE COUNT          338 a          574 c          581 g          292 t
ORIGIN      
        1 gcttccgtta ccaccacctc ttccgcgaca tgctccggtc ggagatgaac ctgcgggacc
       61 ccggcgcgaa acccgggctg caccgccggg ccgccgtctg gctggccgcg aacggtgaca
      121 ttccgagggc gaccgaccac gcgatcgccg ccggggactt cgaactcgcc ggggcctgca
      181 tctggcttga cctgccggac ctgctcagtc gtgggcgggt cgctaccatc gatcgctggt
      241 tcgaaacgat cggggacgac cggatcgcca tgatcccggg cctcttgctg gcccgtgccc
      301 accgcgaaat cgcgatgggc cgcggtgacg agaccttcta ctggctgtca gtcgcggaaa
      361 gggagatcgc tgaagactca cctctctttg gtgacgtgct cacgctccgg gcaaccacgg
      421 ggatcaacgg tgcccgcgga atgattgccg acggggaacg ggcggccggg ctgatgagtc
      481 cgaccagtcc cttccaggtc gcggccaagc tctatgtggg tatcgggctc ggcctgaccg
      541 accaggagaa cgccagcgcg gtgctgaggg agagcgccag ggaggccgcg atgatctcgg
      601 gaatcatcca ggccatcgcc ctgaccgagc tggccctgct ggagctcgac agcggccgcg
      661 tcgatgccgc ccacgactac gcttcccggg cccgggacgt ggtcgaacga agcggattgc
      721 ggatcctcgg ggtgatgtca ctggtctatg tcgtgctggc cctgaccaag gcagaacgcg
      781 gccgggtcga agaggccatc gtggatctcg agaccagttc taggatcggc gaagggatac
      841 tcgattacat cgtctggcat gaggcccaac tggtcctctg ccgctgccgg accctgatcc
      901 ggatcggaca gtttgcccag gcggaaagcg agctcggccg ggcccggatc gcgatcggcc
      961 gggttcccga ttcaccattc ctcgaacgct ggctcgagga atgcgaaggt gagctcgaga
     1021 tggtgcgggg cagcggcggg accacggccg tcagcctgac cagggccgag ttgaggaccc
     1081 tccagttcct gccgagccac cactcgttcc gctcgatcgg cgagcagctt tacctctccc
     1141 agaacaccgt gaagacccaa gccaactccc tctaccgcaa gctcggcgtg aactcgcggg
     1201 ccgaggcagt gatcgaaggc cggcgactcg gattgctcaa gggcgagtga ttaaccccga
     1261 atggatgatg gcccggaacc ggttctggct agatttcgtt caaatcccag ttgaattcga
     1321 ggaaggacag agcagtgacc gaagaaattg aagaaatggg gccgatcgac tacatcctgg
     1381 tcgagtggcc gaacaacgag ccgaccggtg aagcaatccc ctacctggtc gatctggtcg
     1441 acagcggcct gatccggatc ctcgacctcg ccttcatcat gaagaacgaa gacggaagcg
     1501 tcaccgagat ggagatcagc gacctcggcg gcgaatccga atcgttcacc gtgttcgagg
     1561 gcgcctcttc gggcatcctc gatgaatccg accacgccga agcagccgaa gccctggaga
     1621 ccggcaaggc cgcggcgctg ctggtctacg agaacagctg ggccggaccg tttgcttccg
     1681 ccgtacgccg ttccggcggg cagctggttg ccagcggccg gatcccggtc caggccctga
     1741 tcgccagcct cgactcggcc gaagcctaga aacctcacag aagca
//
LOCUS       DAOQFI010000467         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42873_length_1783_cov_2.991319, whole genome shotgun sequence.
ACCESSION   DAOQFI010000467 DAOQFI010000000
VERSION     DAOQFI010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42873_length_1783_cov_2.991319"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1783)
                     /locus_tag="PLE93_08205"
     CDS             complement(<1..>1783)
                     /locus_tag="PLE93_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNH87070.1"
                     /translation="TLLYNLTDKVTVPPTSPPDPSTFDRSLDDDHTTTVVEPDLALDK
                     SVSGDDDQDDSRTTQPGDSYTFSIKVTNNGTSPAYDLTVTDQPDPALTNIQVNHSPVF
                     EVPVLPVNSNDPIQWFIPGPIDPGESVTLTYTADLVPSAQLHNMQQVVNTAELPSYWG
                     VSAGEREDPDNAGIDYREYEGNNDTVTMTVLVPTVTLDKTTGLPQFPDTGDAQTETPF
                     PWRILITNPNTGSSLLGVDLTDTLPPNWTYVPNSAQITGTGTLTPGGQVNPTITQNPT
                     GDVLVWSNIANLVGVQNAVVSFNAIPEPEAAIDPGLTNPHVNEAEAIGEDTSGATASG
                     DGPYTDDDDATATLETPVTDIQIVKTADDPAPTAGTNTTWTLVVKNNGPKVSPDVEVS
                     DVLPAGLTYVSATSSQGTCAENPAGTMHCQIGQMNVDDEVTIHLTTAVGAGTVGQTLT
                     NPADVDDPYIDDSNPANNHSEDDVIPTESADVGIGKSIDEPLFAGQTGSYKLTVTNHG
                     PSVARGVTVTDTLPENLTYAGATTPVGSCSGSGQDFSCDLGDLDPGQTVVIDVSVNVL
                     EGGEYTNCADVASPTPDPDGDNNHSCTT"
BASE COUNT          251 a          512 c          650 g          370 t
ORIGIN      
        1 ggtggtgcag gaatggttgt tgtcgccgtc cgggtccggg gtcgggctgg ccacgtcggc
       61 gcagttcgtg tactccccgc cttcgagcac gttcaccgac acgtcgatca ccacggtctg
      121 gccggggtcg agatcaccga gatcgcagga gaagtcctgt ccggaaccgc tgcagctgcc
      181 gaccggggtc gtggcgccgg cataggtcag gttctccggc agcgtgtcgg tgacggtcac
      241 tccgcgggcg acgctggggc cgtggttggt gacggtcagt ttgtagctgc cggtctggcc
      301 ggcgaagagc ggctcgtcga tcgacttccc gatcccgacg tcggccgact cggtcgggat
      361 cacgtcgtct tctgaatggt tgttggccgg gttcgaatcg tcgatgtagg ggtcgtcgac
      421 atcggccggg ttggtcaggg tctggccgac cgtgccggcc ccgaccgccg tggtcaggtg
      481 gatcgtcact tcgtcatcga cgttcatctg cccgatctgg caatgcatgg ttcccgccgg
      541 gttttccgca caggtcccct gggaggaagt ggcgctcacg taggtgagcc cggccggcag
      601 gacgtcactt acctcgacgt caggcgagac cttgggcccg ttgttcttca ccacgagggt
      661 ccaggtcgtg ttcgtgcccg ccgtcggggc cgggtcatcg gcggtcttga cgatctggat
      721 gtcggtgacc ggagtttcga gggtcgccgt ggcgtcatcg tcgtccgtgt atggcccgtc
      781 tccggaggcg gtagcgccgg aagtgtcttc cccgattgcc tcggcctcgt tcacatgcgg
      841 gttggtcagc cccggatcga tcgccgcttc cggctccggg atcgcgttga aggagacaac
      901 ggcgttctgg actccgacca gattcgcgat gttcgaccag accaggacgt caccggtcgg
      961 gttctgggtg atcgtcggat tgacctggcc gcccggggtc agggtgccgg tgccggtgat
     1021 ctgggccgag ttcggcacat aggtccagtt cggcggcagg gtgtcggtca gatcgacgcc
     1081 gagcaggctg ctgcccgtgt tcggattggt gatcaggatc cgccacggga agggcgtctc
     1141 ggtctgggcg tcaccggtgt cggggaactg cggcagcccg gtcgtcttgt cgagggtaac
     1201 ggtcgggacc agcaccgtca tggtcacggt gtcgttgttg ccctcgtact cccggtagtc
     1261 gatgccggcg ttgtccggat cctcgcgttc cccggccgaa acgccccaat aggaaggcag
     1321 ctcggcggtg ttcactacct gctgcatgtt gtgcagctgg gcggacggca ccagatcggc
     1381 cgtgtaggta agggtgaccg actcgccggg atcgatcggg ccgggtatga accactggat
     1441 cggatcattc gaattgacgg gaaggaccgg cacctcgaag accggcgaat ggttgacctg
     1501 gatgttggtc agcgccgggt caggctggtc ggtgaccgtc aggtcatagg ccggggaagt
     1561 cccgttgttg gtgaccttga tcgagaacgt gtagctgtca ccgggctgcg tggtccggga
     1621 atcgtcttgg tcatcgtcac cggaaaccga tttgtcgagt gccaggtcgg gctcgaccac
     1681 ggtcgtggtg tgatcgtcat cgaggctccg gtcgaaggtg gagggatccg gcggagaggt
     1741 cggcggcacc gtgaccttgt cggtcaggtt gtagagcagg gtc
//
LOCUS       DAOQFI010000468         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42918_length_1782_cov_3.097279, whole genome shotgun sequence.
ACCESSION   DAOQFI010000468 DAOQFI010000000
VERSION     DAOQFI010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42918_length_1782_cov_3.097279"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PLE93_08210"
     CDS             complement(<1..1619)
                     /locus_tag="PLE93_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87071.1"
                     /translation="MPLTIIKGPPNSGRTELVRERFESRIADDPVLVVPSTDDIFGWE
                     RRLTRGSGAFLGGTVVHFKDLIELILGDGSAARSYEKGSDVACPLRRRALATDAIHAG
                     WPAIAGRLEDQPGLVDSALQVIDEFRAALISPKEFDDPAISDLAGPAAGVFRLYLDGL
                     HEAGLTDLPERSVLATTHSLDSWEGRPVFVAGFDDLTRQQLDLLRRLSGKTDVTIAIT
                     HEQGNEAMAITESLLGRLREIGGTVERETERPAEQADHEGLLVELERSFLDPGKAGTI
                     KPGGALRLIEASGRRGEAEAIGAEIARLVSNGTDPGEIAIAIDAPATNGTALLEVLTE
                     YEIPAMLEAETTAPESAIGQSAINFLLATARNGPADRLFAWLRGPLGLDPDLIDQLEF
                     RAVRSGTESAREVIAAMNPAQRRGLPGLAEGENGDPSEAVRSVVRHGARVLLAERQDS
                     IPASSIATETQMASAISRACDELEGIHGDHLSARILIDALASGSVKTWAVPTAHTVRI
                     GSPYAMRAKRVEYLFIASLQEKDLGGDQSSPL"
BASE COUNT          271 a          580 c          618 g          313 t
ORIGIN      
        1 agggggctgc tctgatcgcc accgagatcc ttctcctgaa gcgaagcgat gaacaggtat
       61 tcgacccttt ttgcccgcat tgcgtacggg ctgccgatcc tcaccgtgtg tgcggtcggc
      121 acggcccagg tcttgaccga cccgctagcc agggcatcaa tcaggatccg ggccgagaga
      181 tggtcgccgt gaatcccttc cagctcgtcg caggcacggc tgatcgcact cgccatctgg
      241 gtctcggtgg cgatcgaaga ggcagggatc gaatcctgcc gctcggccag caaaaccctt
      301 gctccgtgtc gcacaaccga tctgaccgcc tcggacgggt ctccgttctc gccctcggcg
      361 aggccaggca gcccgcggcg ctgagccggg ttcatcgctg cgatcacttc ccgcgcgctc
      421 tcggtgcccg agcgaaccgc cctgaactcg agctggtcaa tcaggtcagg gtcgagaccg
      481 agcgggcctc tcagccatgc gaacagccgg tcggcgggtc cgttccgggc ggtggccagg
      541 aggaagttga tcgcggactg gccgatcgcc gattccggtg ccgtcgtttc ggcttcgagc
      601 atcgccggga tctcgtactc ggtgagcacc tccagaagcg cggtcccgtt ggtcgccgga
      661 gcgtcgatgg cgatcgcgat ctctccggga tcggtcccgt tgctgaccag gcgcgcgatc
      721 tccgcgccga tcgcttcggc ttccccgcgg cgccccgagg cctcgatcag tctgagggcg
      781 ccaccgggct tgatcgttcc ggctttcccg gggtcgagga agcttcgctc gagctcgacc
      841 agcaggcctt cgtgatcggc ttgctccgcg ggacgttccg tttcccgttc caccgtcccc
      901 ccgatctccc tgagtcgtcc cagcaggctc tcggtgatcg ccatcgcctc gttgccctgc
      961 tcgtgagtga tcgcgatcgt cacgtcggtc tttccggaga ggcggcggag gagatcgagc
     1021 tgctgccggg tcaggtcatc gaagccggcg acgaatacgg gacgaccttc ccacgaatcg
     1081 agtgaatgag tcgtagccag cacggacctc tcgggcagat cggtcagtcc ggcctcgtgc
     1141 agtccgtcca ggtagaggcg gaagaccccc gcggcaggtc cggccagatc ggaaatggca
     1201 ggatcatcga actccttcgg ggaaatcagg gcggcccgga actcgtcgat tacctgaagc
     1261 gccgagtcga ccagcccggg ctgatcttcc agccggccgg cgatcgcagg ccagcccgcg
     1321 tggatggcgt cggtagcgag cgcgcgacga cgaagggggc aggcgacgtc gctgcccttc
     1381 tcatagctgc gtgccgccga tccatctccg aggatgagtt cgatcaggtc cttgaagtgg
     1441 accaccgttc ccccgaggaa ggctccgctt ccgcgggtca ggcgtcgctc ccagccgaag
     1501 atgtcgtcgg tggagggcac gacgaggacg ggatcgtcgg cgatccggga ctcgaagcgc
     1561 tcgcggacga gttcggtccg gccggagttc ggggggcctt tgatgatggt caggggcacg
     1621 gggcaacttt atggtcggga gcggcgggcg tttcgagtgg cccgagggac aggtctacgc
     1681 cggggaggac gaggtctacc tcgggccgcg cgcggcgatc gttgccgccg gtcggggggc
     1741 gatcgggcgc caaggacggc cggcgagcaa gctcgcggtg tg
//
LOCUS       DAOQFI010000469         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42966_length_1781_cov_2.896292, whole genome shotgun sequence.
ACCESSION   DAOQFI010000469 DAOQFI010000000
VERSION     DAOQFI010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42966_length_1781_cov_2.896292"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PLE93_08215"
     CDS             complement(<1..1245)
                     /locus_tag="PLE93_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNH87072.1"
                     /translation="MESPEVSSRPEPAVPNQGDQPHLKADAISFADGLAIALASTAPA
                     YSLAAVIGSIVVLVGLQAPAALLVAFVPMFFIAAAFYYMNKADQDCGTCFAWVTRAIG
                     PQAGWMTGWAICVTGILVVGSLADVAATSFFDLIGADSLMNSTAAVTGTAVALMIVMT
                     GVCVWGTEISANLQKVLMVVQIVPLILFIVVALFKVWSGDAPAGSVDPSLSWLNPFEI
                     PTGALSGAMLLAVFIYWGWESAVNLNEETEGDVDKPGLAGVISTTLLLITYVGVAFAI
                     VAWGGVSGAGKFDDNAAILATYAKQVLGGTLGLLVLLSVVVSALASTQTTILPSSRTS
                     LSMARAEAMPHALSEISGRFFTPVVSTVTIGVLAILWYLPSKLISENFLFDSLSALSL
                     MIAFYYAMTGYACAFYYRRELFK"
     gene            1405..>1781
                     /locus_tag="PLE93_08220"
     CDS             1405..>1781
                     /locus_tag="PLE93_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH87073.1"
                     /translation="MATTTPESTSIQALQEQARRHLWMHFSRMGGYDDQHEIPIIARG
                     DGAYVYDAHGKRYLDGLSGLFCVNAGHGRTELAEAAARQYEELDFFVIWSYAHPRAIE
                     LATKIASMTPGDLNRVFFTSGGG"
BASE COUNT          359 a          573 c          584 g          265 t
ORIGIN      
        1 cttgaagagc tcccggcggt agtagaaggc gcaggcgtat ccggtcatcg cgtagtagaa
       61 ggcgatcatc agcgagaggg ccgaaaggga atcgaagagg aagttctcgc tgatcagctt
      121 cgagggcagg taccagagga tcgccaggac gccgatcgtc acggtggaaa cgaccggggt
      181 gaagaagcgg cccgagatct cgctgagcgc atggggcatc gcttccgccc gggccatcga
      241 aagcgaggtg cgggaggagg gcaggatcgt ggtctgggtc gaggcgagtg ccgagaccac
      301 caccgaaagc aggaccagca gcccgagggt tccgcccagg acctgcttgg cgtaggtggc
      361 gaggatcgcg gcgttgtcat cgaacttccc ggccccggac acgccgcccc aggcgacgat
      421 cgcgaaagcg accccgacgt aggtgatcag cagcagcgtg gtcgagatca cgccggccag
      481 tccgggcttg tcgacgtccc cctcggtttc ctcgttcagg ttcaccgcgc tttcccagcc
      541 ccagtagatg aacaccgcga gcagcatcgc accggaaagc gccccggtcg ggatctcgaa
      601 ggggttcagc caggaaagcg acgggtcgac cgaaccggcc ggggcatcgc cgctccagac
      661 cttgaacagg gcgacgacga tgaacaggat cagcggcacg atctgcacga ccatcagaac
      721 cttctggagg ttggccgaaa tctcggtccc ccagacgcat accccggtca tcacgatcat
      781 cagggcgacg gcggtgccgg tcactgccgc ggtgctgttc atcaggctgt cggccccgat
      841 caggtcgaag aagctggttg ccgccacgtc ggccagcgag ccgaccacga ggatgccggt
      901 aacgcagatc gcccagccgg tcatccagcc cgcctggggt ccgatcgccc gggtcaccca
      961 ggcgaagcag gttccgcagt cctggtcggc cttgttcatg taatagaagg cggcagcgat
     1021 gaagaacatc ggcacgaagg cgaccagcag ggcggccggg gcctgcagcc cgaccagcac
     1081 gacgatcgag ccgatgaccg cggccaggga gtaggccggg gcggtcgagg cgagggcgat
     1141 tgcgagaccg tcggcaaaac tgatcgcgtc ggccttgagg tgaggctggt cgccctggtt
     1201 ggggacagca ggctccggac gagaagaaac ctctggactc tccatgcggg aaacctagtg
     1261 gcaaacccgg cgccaggccg aaactccgac gtccggtaac ggagcggcgc tcccttttta
     1321 cgttccgtcg acttgcctgt gtgacttacc cgacataggg tttgtccaca cccccaactt
     1381 caccatccgg aaaaggagga gcgcgtggcc accacaactc ccgaatcaac ttcaatccag
     1441 gctctgcagg aacaggctcg ccgccacctc tggatgcact tctcccgcat gggcggttac
     1501 gacgaccagc atgagattcc gatcatcgcc aggggcgacg gcgcctatgt ctacgacgcc
     1561 cacggcaagc ggtacctcga cgggctttcc gggctgttct gcgtgaacgc cggccacggc
     1621 cgcaccgaac tggccgaagc ggctgcccgc cagtacgagg aactcgactt cttcgtgatc
     1681 tggagctacg cccatccccg ggcgatcgaa ctcgccacga agatcgcctc gatgaccccg
     1741 ggcgacctca accgggtctt cttcaccagc ggcggcggcg a
//
LOCUS       DAOQFI010000470         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_42972_length_1781_cov_2.539397, whole genome shotgun sequence.
ACCESSION   DAOQFI010000470 DAOQFI010000000
VERSION     DAOQFI010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42972_length_1781_cov_2.539397"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLE93_08225"
     CDS             complement(<1..501)
                     /locus_tag="PLE93_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNH87074.1"
                     /translation="MFERFTERARQVVVLAQEEARTLKHNYIGTEHILLGLLREEEGL
                     AARVLESLEITVERVRAQVVRIVGSGEEVTSGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRENEGVAARILLDFDADSEKIRNEVIRMLSGPGGRRQGSGGGGQGGA
                     QASGGSG"
     gene            751..1377
                     /locus_tag="PLE93_08230"
     CDS             751..1377
                     /locus_tag="PLE93_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87075.1"
                     /translation="MREHKRGVEFRWLPILAAALVSLALVIPMLAPTPSGAAYPSSFR
                     RHQEQYWVWYAPRSWIASSGKYDLNISSATGTLWNKYGYSAVVCYNPAAWFKYLRNNY
                     RDTAGAGFGIYSKPLSRTHFTRIGRIKQLPKARYGPAYFRQKLLWTGRRKGSGQVIRG
                     EMIMDIFAIDSFGNCAQRFQTRGAPARGNAASIRLLRLVQSTITQRNL"
     gene            1411..>1781
                     /locus_tag="PLE93_08235"
     CDS             1411..>1781
                     /locus_tag="PLE93_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87076.1"
                     /translation="MKRSSVVTFALALATVAALLAPASPASARDEVHRCTNWSWTGPS
                     SWDAVCSKQGISISSPDGRRGIDWGFSNIFCVRANSYRQSAARFIASQRQSINGLRFV
                     RKGRLRQVGRLYFRQTFIVTAG"
BASE COUNT          286 a          590 c          567 g          338 t
ORIGIN      
        1 gcccgacccg ccggaggcct gcgcgccgcc ctggccgccg ccgccggagc cctggcgacg
       61 cccgcccggg ccggacagca tgcggatgac ctcgttgcgg atcttctcgg agtccgcgtc
      121 gaagtcgagc aggatgcgcg cggcgacgcc ctcgttctcg cggacgaggc cgagcaggat
      181 gtgctcggtg ccgatgtagt tgtggccgag gctgagcgcc tcgcgcaggg ccagctccag
      241 caccttcttc gcccgcggcg tgaaggggat ctggccggat gtgacttctt caccggagcc
      301 gacaatccgg accacctgtg cccggacgcg ctcgacggtg atttcaagtg attccaggac
      361 ccgcgcggcg agaccttctt cctcgcgaag cagcccgagc aggatgtgct cggtgccgat
      421 gtagttgtgc ttgagcgttc tcgcttcttc ctgagcgagg acgactacct gacgtgcgcg
      481 ttctgtgaat ctctcgaaca agtttcttct tctacctcaa acctcgatgg ttgcctgagt
      541 atcggtgatt ggtcggtcgg tttttctggt cggtcttgcg agtcggcaaa gtgccggctg
      601 ccctatccct gtttcagtcg tgaaacacct tgggatctga cctccggtct tgggggatcg
      661 agcgaataag ccagtataac gaggcgcctc ctcgggctgg atcggcaatg cggggttgat
      721 cggggcatct tgcggcgtta tggtcggctc atgagggagc acaagcgggg agttgagttc
      781 cggtggctgc cgatccttgc cgcggccctg gtttccctcg cgctggtgat cccgatgctg
      841 gcacccacac cttccggggc cgcctacccg tccagcttcc ggcgccatca ggagcagtac
      901 tgggtctggt acgccccccg gagctggatc gcttcctccg gcaaatacga cctcaacatc
      961 agctcggcga ccggcaccct ctggaacaaa tacggataca gcgccgtcgt ctgttacaac
     1021 cccgccgcct ggttcaagta cctgcgcaac aactaccggg ataccgccgg ggccggtttc
     1081 ggcatctact cgaagccgct gagccgcacc catttcacgc ggatcggcag gatcaagcag
     1141 ttgccgaagg ccaggtacgg ccccgcctat ttccggcaga agctgctctg gaccggccgc
     1201 cggaagggca gcggccaggt gatcaggggc gagatgatca tggacatctt cgcgatcgac
     1261 tctttcggaa actgcgccca gcgattccag acccgcggcg ccccggcccg tggaaatgcg
     1321 gcaagcatca ggctgctgcg cctggttcag tcgacgatca cccagcgaaa cctgtgaggc
     1381 aacgctgccg gtgaggttag gcttgcgaac atgaagcgct cgtccgtcgt cactttcgcc
     1441 cttgcccttg cgaccgttgc cgcgctgctg gcgccggcct cccccgcctc ggcccgggac
     1501 gaggtccacc gctgcaccaa ctggtcctgg accgggccgt cgagttggga cgcggtctgc
     1561 agcaagcagg gcatcagcat ctcctcgccg gacgggcgcc gcggaatcga ctggggcttc
     1621 agcaacatct tctgtgtccg cgcgaacagt taccggcagt cggcggcgag attcatcgcc
     1681 agccagcgcc agagcatcaa cggacttcgc ttcgtgcgca agggccggct gcgccaggtt
     1741 ggccggctct acttccgcca gaccttcatc gtcacggcgg g
//
LOCUS       DAOQFI010000471         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_43222_length_1776_cov_3.079605, whole genome shotgun sequence.
ACCESSION   DAOQFI010000471 DAOQFI010000000
VERSION     DAOQFI010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43222_length_1776_cov_3.079605"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLE93_08240"
     CDS             complement(<1..840)
                     /locus_tag="PLE93_08240"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNH87077.1"
                     /translation="MPGLDGLRGVAVLAVIAYHLKIDWASGGLLGVGVFFTLSGYLIT
                     DILLERWSERRLVLKEFWLARARRLLPALFVVLIVVMAWVTIGNPAQLSTLRGETVAS
                     ALFFSNWWFIFQDVSYFEQFGPPSPLGHIWSLGVEEQFYIFWPWILLAGLAVFGVGQK
                     ARRGVRQRVQPRLALATLGLALVSIILMAVLFTPGPDSTRAYEGTDTRAFGLLIGAAL
                     AMVWPSRRLVGKVSLQARNTLDLVGAVGLVSILILIATTDEFSPFIYRGGLVLLSIAT
                     AMVV"
     gene            1001..1588
                     /locus_tag="PLE93_08245"
     CDS             1001..1588
                     /locus_tag="PLE93_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH87078.1"
                     /translation="MRRIDREIQSWSRDSARVQLALLWDEVERDGVRSISWWDGDPWA
                     SAYLISSTQPWSGGIGKWRAFCMINFGRPEDDEILFSDDREVVGRTSPAHGMGAREIR
                     RMLELPGVEERKELCDLLIALGHRRDELDIPTITGFAGHEDRVVRYVVLAMLSWNPSI
                     ANDYLAGKFSDDPDPLVRELARRWSGGRLRRRTRA"
BASE COUNT          335 a          567 c          616 g          258 t
ORIGIN      
        1 gaccaccatc gcggtcgcga tcgaaagcag caccagcccg ccccggtata tgaagggcga
       61 gaactcgtcg gtggtcgcga tcaggatgag aatcgagacc aggccgaccg cgccaaccag
      121 gtcgagcgtg tttcgggcct gaagcgagac cttgccgacc agcctccggc tcggccagac
      181 catcgccagg gcggctccga tcaggaggcc gaaggcgcgg gtgtcggttc cctcgtaggc
      241 cctggtcgag tcggggccgg gcgtgaagag gaccgccatc aggatgatcg agacaagagc
      301 gagaccgagg gtggccaggg cgagccgggg ctggacccgc tgccgcacgc cgcgccgtgc
      361 cttctggcca accccgaaaa cggccagtcc cgccagcagg atccacggcc agaagatgta
      421 gaactgctcc tccaccccga gggaccagat gtgcccgagc ggggagggcg ggccgaactg
      481 ttcgaagtac gaaacgtcct ggaagatgaa ccaccagttg gagaagaaga gggccgaggc
      541 gacggtttcg ccccgcagcg tggagagctg ggccgggttg ccgatcgtca cccaagccat
      601 gaccacgatc aggaccacga aaagggcggg cagaaggcgc ctggcccggg ccagccagaa
      661 ctccttgagc acgagccggc gctcggacca gcgctcgagc aggatgtcgg tgatcaggta
      721 accgctgagg gtgaagaaca ccccgacgcc cagcagtccg cccgaggccc agtcaatctt
      781 gaggtggtag gcgatgaccg ccagcacggc cacgccgcgc agcccgtcca gccccggcat
      841 gtaccgctga gaactctgtc caatcggttt cggcatctac ggcgttggtt cctgcggttg
      901 cgggtgcccg gaaccggtaa ctctagttcg cggacggggc gggagatctg cacaatcacc
      961 gccgcggccg gaacccttag gctgccagcg ggagacgatc atgaggcgta tcgaccggga
     1021 gatccagtca tggagccggg actctgcgcg agtccagctt gccctgctct gggacgaagt
     1081 cgaacgggac ggcgtcaggt cgatctcctg gtgggacgga gacccgtggg cttccgcgta
     1141 tctgatctcc tcgacccagc cctggagtgg cggcatcggg aagtggaggg ccttctgcat
     1201 gatcaacttc ggccgtcccg aggatgacga gatcctcttc tcggatgatc gtgaagtggt
     1261 gggaaggacc agtcccgccc acggaatggg tgcccgcgag attcgccgga tgctcgagct
     1321 gcccggcgtg gaggaacgga aggaactctg cgacctgctg atcgccctgg gtcatcgccg
     1381 ggacgagctt gacatcccga cgatcaccgg gttcgccggg cacgaagacc gggtggtccg
     1441 ctacgtcgtg ctcgcgatgc tgtcctggaa cccttcgatc gccaacgact atctggcggg
     1501 caagttcagc gacgaccccg atccgctcgt ccgggaactt gcccggcgct ggtccggtgg
     1561 caggttgcgc cgccggaccc gcgcctgagc cggaacccga gcctgacccg gaagcgcgag
     1621 tcaggacctg agttctgcca gcaaagcctg cggagtgagg atccggactc cccggtgccg
     1681 atccaccggc agcaggtgct gccggtccct ggagacgagc acggtggccg acgattcgac
     1741 ggcgcaggcg aggatcaggt catcgtccgg gtcacc
//
LOCUS       DAOQFI010000472         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_43224_length_1776_cov_2.943056, whole genome shotgun sequence.
ACCESSION   DAOQFI010000472 DAOQFI010000000
VERSION     DAOQFI010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43224_length_1776_cov_2.943056"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..738
                     /locus_tag="PLE93_08250"
     CDS             94..738
                     /locus_tag="PLE93_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87079.1"
                     /translation="MIGGYVLQDPGMGSLAGRYLYGDLCNPNLRTLDLDAAGADPKPA
                     GFRIPAGGGTLRSFGEDSAGCVYALTSLAVFRVVSDTATGSGCPGPADAVPAATYNSF
                     IPHRQVIGKRITVAAKCSIACAASATAKVRISRNRFRRNQTVFNLKAGTRNLIAGTRS
                     SLFFRLPSARVATMKKAAGNGSRVTAWVKVSMTGADSSGGSGSGQIRLTRPVSR"
     gene            749..>1776
                     /locus_tag="PLE93_08255"
     CDS             749..>1776
                     /locus_tag="PLE93_08255"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNH87080.1"
                     /translation="MKWITGAGVVLVCWLGLSGSAFGASLVRVTPEGYLGSDPSFVTA
                     PPGDSRVFLAERGDAGTHQARIRIIKDGVPVTEPFLTIGNVDLASERGLLSIAFAPDY
                     ASSGLFYAFYVAHGADGFGGNTGDIRIVEYKVSAGDPDQADPASARLVFSTPHSAGNH
                     NGGWMAFGPDGDLYFSIGDNANSSNAQALANYYGKIMRIHPADPDGAGPATYSVPTDN
                     PFTSQAGAKKEIYALGLRNPYRASFTPDGRLVIGDVGNGTWEEVDVGDLKGANLGWPT
                     CEGFCTGLTPSFVAPIYAYNHDNGVDGQGGGCAVIGGYVVRDETLGGLTGRYLYGDLC
                     DQALRTLDL"
BASE COUNT          284 a          629 c          572 g          291 t
ORIGIN      
        1 tgtcgctttg atcggcggct cgagtaagac gggccgaacc cgcttctcac cgacccgatc
       61 cactcatacg cgcatgacgc gagcaactgt gcggtgatcg gcggctacgt gctccaggac
      121 cccggcatgg gctcgcttgc cggtcgatac ctctacggcg acctctgcaa tccgaacctc
      181 cgcacgctcg acctcgacgc ggccggggcc gacccgaagc cggccggttt caggatcccc
      241 gccggtggcg gaacgctgcg ttccttcggt gaggattccg ccggttgcgt ttacgcgctg
      301 accagcctcg ctgtcttccg ggtggtcagt gacaccgcca cagggtccgg ctgcccgggc
      361 cccgccgacg cggtgccggc cgcgacctac aactcgttca ttccccaccg ccaggtgatc
      421 ggcaaacgca tcacggtcgc cgcgaagtgc tcgattgcct gcgccgcctc ggccaccgcg
      481 aaggtccgga tcagccggaa ccggttcagg aggaaccaga cggtcttcaa cctgaaggcc
      541 ggaacccgga acctgattgc cggaacccgc agcagcctct ttttccggct gccgtcggct
      601 cgggtcgcca cgatgaagaa ggcggccggc aacgggagcc gcgtcaccgc ctgggtgaag
      661 gtctcgatga ccggcgcgga cagctccgga ggatcgggta gcgggcagat ccgtttgacc
      721 agacccgttt cgcggtagtt tcgtaaccat gaagtggatc actggggcgg gggttgtgct
      781 ggtttgctgg ctggggctca gcggttcggc cttcggggcg agcctggtcc gggtcacccc
      841 cgagggatat ctcggctccg atccgagttt cgtgaccgcg cctcccggtg actcgcgggt
      901 cttcctggcc gagcggggcg atgcgggcac ccaccaggcc cggatccgga tcatcaagga
      961 cggggtgccg gtgaccgagc ccttcctcac gatcggcaac gtcgacctgg ccagtgaacg
     1021 ggggctgctc tcgatcgcct tcgcccccga ttacgcatcc agcggtctct tctacgcctt
     1081 ctacgtcgcc cacggggcgg atggcttcgg cggcaacacg ggcgacatca ggatcgtcga
     1141 gtacaaggtc tcggccggtg atccggacca ggccgacccc gcctcggccc ggctcgtgtt
     1201 cagcaccccc cactcggccg gcaaccacaa cggcggctgg atggccttcg gcccggacgg
     1261 cgacctctac ttctcgatcg gtgacaacgc caacagctcg aacgcccagg ccctggccaa
     1321 ctactacggc aagatcatgc ggatccaccc ggccgatccc gacggggccg ggccggccac
     1381 ctactccgtg ccgaccgaca atcccttcac ctcacaggcc ggagcaaaga aggagatcta
     1441 cgcgctcggc cttcgcaacc cctaccgggc ctcgttcaca cccgacggcc ggctggtgat
     1501 cggtgacgtc ggcaacggaa cctgggagga ggtcgacgtc ggcgacctga agggcgccaa
     1561 cctgggctgg ccgacctgcg agggcttctg caccggactc accccctcct tcgtcgcgcc
     1621 gatctatgcc tacaaccacg acaacggcgt cgacggccag ggcggtggct gcgcggtgat
     1681 cggcggttat gtcgtccggg acgagacgct tggagggctg accggccgct acctctacgg
     1741 agacctttgc gaccaggcgc tccggaccct cgacct
//
LOCUS       DAOQFI010000473         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_43269_length_1775_cov_2.934302, whole genome shotgun sequence.
ACCESSION   DAOQFI010000473 DAOQFI010000000
VERSION     DAOQFI010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43269_length_1775_cov_2.934302"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..813
                     /locus_tag="PLE93_08260"
     CDS             145..813
                     /locus_tag="PLE93_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87081.1"
                     /translation="MNQSTITVQTIGNLTIDRTDQTVHVDGKKVPTLLREFQLIDQLA
                     RCEAPATFEKLGRKAFAASELRTAGAVEAVAHSLRAKLTSYGARRGLVHINGNKISMF
                     TSPQKVRVQAFHVPSARYTAGPEDGSVIPDLSLCDLIWFGELPESDDRTVLEQTFHLL
                     NIDTRPNRLFAPALTVGDIVTLQPGGAEAVSYQVDHKGWIRLRGTVQAAEADLDLVEN
                     QLAA"
     gene            846..>1775
                     /locus_tag="PLE93_08265"
     CDS             846..>1775
                     /locus_tag="PLE93_08265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNH87082.1"
                     /translation="MDGVQKSREILTDLDPDQRAAVTARNEWPLLVAAGPGAGKTRVL
                     TRRIAWLIEATGIPAGQVMALTFTNAAAKEMGERLYNLIGAESIRCRLSTIHALAARI
                     VRAHAEKVGLTHDFTIFDQDAQLKLAATVLEDGDSGIEPADLVRQVSLEKSRLREPAQ
                     LKLENRSQLAKAWEEFDQAQTKANGLDFEGLVVKAIELLGNQRIRTGWRNGISAVLVD
                     EFQDVSEAQFELLRLLAGDDGAGLSVIGDEDQSLYSWRGASTALLTGFEQHFPQCQVI
                     NLACNYRSHEAIVGTARRLIEHNEGRRGKELVAH"
BASE COUNT          406 a          584 c          505 g          280 t
ORIGIN      
        1 cgcgatcgcg atgacgcttg gcgcgctcag cctcgcagga atcatcggct gacccggaca
       61 ccccgaaaac ccaacatcca tccacattca aaggcggcca gcaggccgcc tttttcatgc
      121 ccgaaaccgg gcaaaggagc aaacatgaac caatccacca tcacggttca gacgatcgga
      181 aacctgacga tcgaccgaac cgaccagacc gttcacgtcg acggaaagaa agtgccaacg
      241 ttacttcgcg aattccagct gatcgaccag ctggcgcggt gcgaagcgcc ggccactttc
      301 gagaagctcg gccgcaaagc attcgccgca agcgaactcc gaacagccgg ggccgtggaa
      361 gcggtcgcgc acagcctccg cgcgaaactc acttcctacg gtgcccgccg gggactcgtt
      421 cacatcaacg gcaacaagat cagcatgttc acctcaccgc agaaagtgag agtccaggct
      481 ttccatgttc cctcggccag atacaccgcc gggccggaag acggcagcgt aatcccggac
      541 ctgtcgcttt gcgatctgat ctggttcggg gaacttcccg aatccgatga ccggacggtg
      601 ctcgagcaga ccttccatct gctgaacatc gacacccgcc ccaaccggct ctttgcccct
      661 gccctcacgg tcggggacat agtcaccctg cagcccggtg gtgccgaagc ggtcagctat
      721 caggttgacc acaaaggctg gatccggctt cgaggcaccg tccaggcggc ggaagccgac
      781 cttgacctgg tcgaaaacca gctcgccgcc tagaccgcct gaccctttca acgaaaggag
      841 gctccatgga cggtgtccaa aagagccgcg agatccttac cgatctcgat cctgaccagc
      901 gggcagcggt aaccgcccga aacgaatggc cgctactggt cgcggccggg cccggtgccg
      961 gcaaaacccg ggtactcacc cgtcggatcg catggcttat cgaagcgacc ggcatacctg
     1021 ccggccaggt aatggcgctc acgttcacga acgcggccgc caaagaaatg ggggaaaggc
     1081 tctacaacct gatcggcgcc gaatcaatcc ggtgccggct ctcaacgatt cacgctctcg
     1141 ccgcccgcat cgtccgggca catgccgaaa aggtcggttt gacccatgac ttcacgatct
     1201 tcgaccagga cgcgcagctg aagctcgcgg ccacagtgtt ggaggacggg gactcaggaa
     1261 tcgaaccggc cgacctggtc cggcaggttt cccttgagaa atcccggctc agggaaccgg
     1321 cccagctgaa actcgaaaac cgctcgcaac tcgccaaagc atgggaagag ttcgaccaag
     1381 ctcaaacgaa agcgaacgga ctcgacttcg aagggctcgt tgtgaaagca atcgagctgc
     1441 tcggcaacca gaggatccgg accggctggc gaaacggcat cagtgccgtg ctcgtcgacg
     1501 agttccagga cgtatccgaa gcccagttcg aactgctccg gctgctggcc ggtgacgacg
     1561 gtgccggact atcggtgatc ggtgatgaag accagtcgct ttactcatgg cgcggagcat
     1621 caaccgcgct actgaccggc ttcgaacagc atttcccgca atgccaggtg atcaaccttg
     1681 cctgcaacta ccgctcgcat gaagcgatcg tcggcacggc ccgtcgcctg atcgaacaca
     1741 acgaggggag gcgaggaaaa gaactcgtcg cccac
//
LOCUS       DAOQFI010000474         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_43270_length_1775_cov_2.909302, whole genome shotgun sequence.
ACCESSION   DAOQFI010000474 DAOQFI010000000
VERSION     DAOQFI010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43270_length_1775_cov_2.909302"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLE93_08270"
     CDS             <1..1002
                     /locus_tag="PLE93_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNH87083.1"
                     /translation="MDTFVDSSWYFLRYLDAQNSEAAWDREAADFWMPVDQYIGGVEH
                     AILHLMYARFFAKALKDMGHIGVVEPFANLFTQGMITRDGAKMSKSKGNTVSPSEIVA
                     RHGADAARTYVCFMGPPERSGDWTDEGVEGVHRFLARLWRLGEELAAAGQSGPLVLPD
                     GLDGEAREVAAKAHWAIDKATGDFNRGFQFNTVIAAVMELVNEIYLVKGNLLAAGGDG
                     AEAVRFATGTAASLIQPFAPHLAAEVFERVTGQRVWEAAWPEADPDLLKSDTVTIVVQ
                     VGGKVRDRVEVADGAPEEEILATAKAAENVARHLEGMTIVKEIVVPGKLVNLVVKPA"
     gene            1106..1747
                     /locus_tag="PLE93_08275"
     CDS             1106..1747
                     /locus_tag="PLE93_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH87084.1"
                     /translation="MPQINRQMLVYAVVGGVLLLVGFNSIRGEKSESASFGGDPAGGG
                     SRAESGADGGSGSGGFEVSGSSRKLIVDVAGAVRRPGVYRFSQGARVIDAIERAGGTT
                     RGAFVGAINRAATLSDGQQVIVPSATEAGAAGGTVSTGGTVSGTEAASAPVSLGTATQ
                     EQLEEIDGIGPVTAEKILEFRDAQGGLGSIDELDQISGIGPVTMESLRAALTP"
BASE COUNT          305 a          567 c          632 g          271 t
ORIGIN      
        1 atggacacct tcgtcgattc gtcctggtac ttcctgcgct atctggacgc gcagaactcc
       61 gaggccgcct gggaccgcga agccgccgat ttctggatgc cggtcgacca gtacatcggc
      121 ggagtcgagc acgcgatcct ccacctgatg tacgcccgct tcttcgccaa ggcgctgaag
      181 gacatggggc acatcggtgt ggtcgagccg ttcgccaacc tcttcacgca ggggatgatc
      241 actcgcgacg gtgccaagat gtcgaagtcg aagggcaaca cggtcagtcc ttccgagatc
      301 gtcgcccgcc acggggccga cgccgcccgg acctacgtct gcttcatggg cccgccggag
      361 cgttccggcg actggaccga cgaaggggtg gagggcgtcc accgtttcct ggccaggctc
      421 tggcgactcg gcgaggagct ggctgccgcc ggccagtccg ggccgctggt gttgccggac
      481 gggctcgacg gcgaggcccg cgaggttgcg gccaaggccc actgggcgat cgacaaggcg
      541 accggtgact tcaaccgcgg cttccagttc aacacggtca tcgccgccgt gatggagctg
      601 gtaaacgaga tctacctggt caaggggaac ctgctcgcgg ccggcggcga cggagcggag
      661 gcagtgcgtt tcgcgaccgg gaccgcagcc tccctgatcc agcccttcgc gccccatctg
      721 gccgccgaag tcttcgaacg agtgaccggc cagagggtct gggaagctgc ctggcccgaa
      781 gccgatccgg acctgctcaa gtccgacacg gtgaccatcg tcgtccaggt cggaggcaag
      841 gtgcgcgacc gggtcgaggt cgccgacggt gcccccgaag aagagatcct cgcgacggcc
      901 aaagccgccg aaaacgtcgc ccgccacctc gaaggaatga ccatcgtcaa ggagatcgtc
      961 gtccccggca agctggtcaa cctggtcgtc aaaccggcct gaagtagccc gaaaccgtcg
     1021 gagtgagccg gaaacggggc tccacgttac tgaatcgttg cacctgtaga gccacgtgtg
     1081 aggtttttcg tggcagtttc ggggcatgcc gcaaatcaac cgacagatgc tcgtttacgc
     1141 cgtggtcggc ggcgtgctgc tgctggtcgg cttcaactcg atccgggggg agaagtccga
     1201 gtcggccagc ttcggcgggg atcccgcggg tggcgggtcc agggcggaat cgggagcgga
     1261 tggcggctcc ggaagcggcg gcttcgaggt cagtggcagt agccgcaaac tgatcgtcga
     1321 cgtggccggg gcggtgcgga gacccggggt ctaccggttc tcccagggtg cccgggtgat
     1381 cgacgcgatc gagcgggccg gtggcaccac acgcggggcc ttcgtcgggg cgatcaaccg
     1441 ggcggcgacc ctgagcgacg gccagcaggt gatcgtgccc tcagcgacgg aggccggcgc
     1501 agccggtgga acggtttcga ccggcgggac cgtttccgga accgaagcag ctagcgcccc
     1561 ggtcagcctc ggaacggcga cccaggagca gctcgaggag atcgacggga tcgggccggt
     1621 gaccgcggag aagatcctcg agtttcggga tgcacagggt gggctcggat cgatcgacga
     1681 gctcgaccag atcagcggca tcgggccggt aaccatggag tcgctccggg cggccctcac
     1741 gccgtgaaca ccgccgccga ctggcggctc gcggc
//
LOCUS       DAOQFI010000475         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_43580_length_1768_cov_2.401635, whole genome shotgun sequence.
ACCESSION   DAOQFI010000475 DAOQFI010000000
VERSION     DAOQFI010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43580_length_1768_cov_2.401635"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLE93_08280"
     CDS             complement(<1..641)
                     /locus_tag="PLE93_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87085.1"
                     /translation="MTKRNDKSITVAICHHDPQVIDELHHEIGGPSNLRFIESPVPSG
                     LRFIARPHSTLKVLVIDQKVEPRESENIWEETALMNPETLVVVIARSGRDRKRIHGTA
                     HRIVLGPNATGKQIATAIRQLRVTRGQEARQAHIEGVIWVDPSICRVRVHDRDVPMSL
                     EEFHMLMYLAADPYETVTKVELAQRLLNGVKTPSGSNIREIEKTARKLKRTLA"
     gene            complement(738..1364)
                     /locus_tag="PLE93_08285"
     CDS             complement(738..1364)
                     /locus_tag="PLE93_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87086.1"
                     /translation="MEKTRLPETGQAAPKPLSTPGKEVTVASALAGRPIHLPRTSSFD
                     VILETHIYDPSSVMGFLTGYRKPAEIDVIKAIRNGPGTKIGTVLGGGQALKLLLAARE
                     SGGEVTFRISTSQIKLGDEFEPDLRLASITMMGCERFTSDHGWLVCEIAGYDRSPDQL
                     AAHINENAWKIVNVIEQVPLPVAAATHDLLNSKGFSNVSVRQAVPRTR"
     gene            complement(1449..>1768)
                     /locus_tag="PLE93_08290"
     CDS             complement(1449..>1768)
                     /locus_tag="PLE93_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87087.1"
                     /translation="FDGIYSQISRDSQETLEATGTLLMVVTPEPWQREGVASRNCSID
                     WLNASQAETSSQIKKQTDVMVNDLAFYSEIREIRTFGVELKNGGLFELAWEIPVPAQL
                     ATA"
BASE COUNT          274 a          501 c          580 g          413 t
ORIGIN      
        1 tcggcgaggg tgcgcttcag cttccgggcg gtcttttcga tctccctgat gttgctgccc
       61 gacggtgtct tgaccccgtt gagcaaacgc tgggcaagct caactttcgt gacggtttcg
      121 taggggtcgg ctgccaggta catgagcatg tggaactcct ccaagctcat gggaacgtcc
      181 cggtcgtgaa cccggacccg gcagatggac gggtcaaccc agatcacacc ttcgatgtgg
      241 gcctgccgtg cttcctggcc tctggtgacc ctgagctgcc ggatcgcggt ggcgatctgc
      301 ttgccggtgg cattggggcc gagcacgatc cgatgggcgg tgccgtggat ccgtttgcgg
      361 tccctgcctg agcgggcgat cactacaacc agcgtttcgg ggttcatcag ggcggtttct
      421 tcccagatgt tttcgctttc gcgcggctca accttctggt cgatcaccag caccttcagg
      481 gtgctgtgcg gccgggcgat gaacctgagt ccggacggga ccggtgattc gatgaagcga
      541 aggttcgaag gtccgccgat ctcgtggtga agctcgtcga tcacctgggg gtcgtgatgg
      601 cagatggcga cggtgatgct cttgtcgttt cgcttggtca tttctgactc tctttctggc
      661 aggggtgttt gccctgccgg tgctgcaggt ttcgtgcggg aaaaggtccc gcggcctgat
      721 tggttgggat gccccgtcta gcgggtgcgg ggtactgcct ggcggaccga cacgttgctg
      781 aagcctttgc tgttcagcag gtcatgggtg gcggcggcaa ccggcagagg cacctgttcg
      841 atcacgttca cgatcttcca ggcgttctcg ttgatgtgcg cggccagctg atccgggctg
      901 cggtcgtaac ctgcgatttc gcagaccagc cagccgtgat cggaggtgaa cctttcgcaa
      961 cccatcatgg tgatagatgc taggcgcagg tcaggttcaa attcgtctcc gagcttgatc
     1021 tggctggttg agatccggaa cgtgacttcg cctccgcttt cgcgggcggc aagcagcagc
     1081 ttcaatgcct ggccgccgcc gagaaccgtt ccgatctttg tgccgggacc gttccggatc
     1141 gctttgatca cgtcgatctc ggcgggtttc cggtaaccgg tcaaaaatcc catcacgctg
     1201 gacgggtcgt agatatgagt ttcgagaatc acatcgaaac tcgaggttcg cggaaggtgg
     1261 atcgggcggc cggcaagtgc cgaggcaacc gtcacctcct tgccgggggt ggacaggggt
     1321 ttcggggctg cctgacctgt ttcgggcagc cgtgtttttt ccaatgtcgc catcggaatc
     1381 tccttcatgt ttggaccgga cggttgccgg tcgaggggtg cgggagaaag tcccgcggct
     1441 tgaactgtct acgcggtggc cagctgcgcg ggaaccggga tctcccaggc cagctcgaac
     1501 aggccgccgt tcttgagttc cacaccgaat gtgcggatct cacggatctc gctgtagaag
     1561 gcgagatcgt tcaccatcac gtcagtctgc tttttgatct ggctgcttgt ttcggcctgg
     1621 ctggcgttca gccagtcgat cgagcagttc cgggaggcga cgccttctcg ctgccagggt
     1681 tccggggtga caaccatcag caaggtgccg gtcgcctcga gcgtttcttg gctgtcccgg
     1741 ctgatctggc tgtagatgcc atcgaaca
//
LOCUS       DAOQFI010000476         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_43673_length_1766_cov_2.970777, whole genome shotgun sequence.
ACCESSION   DAOQFI010000476 DAOQFI010000000
VERSION     DAOQFI010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43673_length_1766_cov_2.970777"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLE93_08295"
     CDS             complement(<1..336)
                     /locus_tag="PLE93_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH87088.1"
                     /translation="MGAADEAPRNRSLPEPVTAVTDTAGDWLPARMAEVEAGLLAAVE
                     GETGLAGDAVRTLNAGGKRMRPMLVLLCAGPEAGPAAVRSGIAVELIHMASLVHDDVL
                     DRAPLRRGLP"
     gene            complement(349..1071)
                     /gene="ubiE"
                     /locus_tag="PLE93_08300"
     CDS             complement(349..1071)
                     /gene="ubiE"
                     /locus_tag="PLE93_08300"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNH87089.1"
                     /translation="MNQAPPRRGDQEFAGEVNSMFDRISGVYDRMNRVMTAGLDQSWR
                     ARAAERARLEPGMKALDLCCGTGDLALMLAERVGPEGEVTGADFSRPMLDIALEKAKS
                     EGLRQARFKWADALDLPFEDESFDALTIAFGARNLADLDRGISEMRRVLVPGGRLVIL
                     EITQPKRQPLAGFFALWFDRIVPQLGKLAGDSSAYTYLPESVRSFPDAETLAGKLDAG
                     GLRHVRWTLMAGGIIALHSGVR"
     gene            complement(1079..>1766)
                     /locus_tag="PLE93_08305"
     CDS             complement(1079..>1766)
                     /locus_tag="PLE93_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNH87090.1"
                     /translation="LREYEILKKKGKPFFPYAIMKDSVMMLIVAGVLVAMSIIMGAEQ
                     GPKADPTTTTYTPRPEWYFFYLFELLRVIKPPALVPVATIGIPTICMVLLLLLPFYDR
                     GPQRVPWKRPIATTTGIMVIIAMALLTYKGAVAGSPSEIELATSPQYEKGKAIVAESG
                     CLACHAIGENGAHGGLGPDLTDIGSKIPKEAIKRSVAVGPGIMPSFQGLGEENLNEVA
                     DYLASLDGEE"
BASE COUNT          285 a          592 c          601 g          288 t
ORIGIN      
        1 gggcagtccg cgccgtagcg gcgcccggtc gagcacgtca tcgtggacca gactggccat
       61 gtgaatcagc tcgaccgcga tccccgaacg gaccgcagcc gggcctgcct ccgggccggc
      121 gcaaagcagg accagcatcg ggcgcatgcg cttgccgccc gcgttgaggg tgcggaccgc
      181 gtccccggcc agcccggtct ccccttcgac cgcggccagc agtccggctt cgacctccgc
      241 catgcgggcc ggtagccagt ccccggcggt gtcggttaca gcggtgaccg gctccgggag
      301 cgaccggttc cggggggctt cgtcagccgc gcccatggcc gagctatctc accggacccc
      361 ggagtgaagg gcgatgatcc cgcccgccat cagggtccag cggacatgcc ggaggccccc
      421 ggcgtcaagc ttgccggcca gggtctccgc atcggggaaa ctgcgcaccg attccggcag
      481 gtaggtgtag gcggaggagt cacccgcgag cttgccgagc tgaggcacga tccgatcgaa
      541 ccagagggca aagaagccgg ccaggggctg gcgcttcggc tgggtgatct cgaggatgac
      601 caggcgaccg cccggcacca ggacccggcg catctcgctg attccccggt cgaggtcagc
      661 caggttgcgg gcgccgaagg cgatcgtcaa ggcgtcgaag gattcatcct cgaagggcag
      721 gtcgagggcg tcggcccact tgaaccgtgc ctgcctcagc ccttcgcttt tggctttctc
      781 aagcgcaatg tcaagcatcg ggcgggagaa gtccgcgccg gtcacttcac cttccgggcc
      841 gaccctttcg gccagcatca gggcgaggtc accggtgccg cagcaaaggt cgagcgcctt
      901 catgccgggt tcgagccggg ccctttcggc cgcgcgcgcc cgccagctct ggtcgaggcc
      961 ggcggtcatc acccggttca tccggtcgta cacaccgctg atccggtcga acatcgagtt
     1021 cacctcgccg gcaaactcct ggtcaccccg gcggggaggt gcctggttca ttggacggct
     1081 actcttcgcc gtcgagcgaa gcgaggtagt cggcaacctc gttcaggttc tcttcaccga
     1141 gaccctggaa ggagggcatg atgcccgggc cgaccgcgac cgaacgcttg atcgcttcct
     1201 tcgggatctt cgacccgatg tcggtcaggt ccggaccgag accgccgtgg gccccgttct
     1261 cgccgatcgc gtggcaggcc agacagccgg attcggcgac gatcgccttg cccttctcgt
     1321 attgcggcga ggtggcgagt tcgatctcgc tgggcgagcc ggccaccgct cccttgtagg
     1381 tcagcagcgc catcgcgatg atcaccatga tgccggtggt ggtcgcgatc ggacgcttcc
     1441 acggcacccg ctggggtcca cggtcataga agggcagaag cagcagcagg accatgcaga
     1501 tggtcgggat gccgatcgtg gcgaccggaa ccagggccgg cggcttgatc acgcgcagca
     1561 gctcgaagag gtagaagaag taccactcgg ggcgcggcgt gtaggtggtg gtggtcgggt
     1621 ccgccttggg cccctgttcg gcacccatga tgatcgacat cgcgaccagc acgccggcga
     1681 cgatcagcat catcaccgag tccttcatga tcgcgtaggg gaagaacggc ttgcccttct
     1741 tcttcaggat ctcgtactcg cggagg
//
LOCUS       DAOQFI010000477         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_43722_length_1765_cov_3.443860, whole genome shotgun sequence.
ACCESSION   DAOQFI010000477 DAOQFI010000000
VERSION     DAOQFI010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43722_length_1765_cov_3.443860"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLE93_08310"
     CDS             complement(<1..1158)
                     /locus_tag="PLE93_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH87091.1"
                     /translation="MDFEAGSEVEGLLERVRSFMDEHIYPREAELHQAIDDEVKAGVA
                     YPVELEQIREKAKSEGLWNLFLPDEHYGAGLNNWQYAFLCEEMGRSPLVAPMAFNSAA
                     PDTGNMEILLEHGNDEQKAKYLAPLTDGEIRSCFSMTEPDTAGSDPTGLRAKAELVDG
                     EWVINGHKWFTSGAVGAEFAIAMVNTEESDNPYANASMILVPLDAPGFNLVRPVPVMG
                     HSKGPGHCEILYEDCRVPEENLLGARGAGFLIAQDRLGPGRIHHCMRAIGTAERALEM
                     MCLRAADREAFGGPLADKQFVQDFIARSRMEIDQARLLSLNAAWMMDTVGKHAARQEI
                     SMIKVVAANMVMDVLDRALQVHGALGMSDDTPLAAMWRYSRMLKLADGPDEV"
     gene            complement(1195..>1765)
                     /locus_tag="PLE93_08315"
     CDS             complement(1195..>1765)
                     /locus_tag="PLE93_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH87092.1"
                     /translation="LGSNADTEHLLGLRTRFDAVLIGGGTMRAERYGRIISRPEYRRR
                     REAAGLDGDALAVIISGDLDLPFDAPLFTNGEGRVLIFSRKKSLPETETPVELVTIDG
                     LIDSAEVLAYLRRNEGVRAVLCEGGPRVFGQLVAGDLVDELFLTMTPVATGGDATHIL
                     EANLPREARFELTGLLEADGDLFTRYRRA"
BASE COUNT          283 a          579 c          590 g          313 t
ORIGIN      
        1 gacctcgtcg ggaccgtcgg cgagcttaag catgcggctg tagcgccaca tcgcggccag
       61 cggagtgtca tccgacatgc cgagggcgcc atggacctgg agggcccggt caagcacgtc
      121 catcaccatg ttggcggcga ccaccttgat catcgagatc tcctggcggg cggcatgctt
      181 gccgaccgtg tccatcatcc aggcggcgtt cagcgaaagc aggcgggcct ggtcgatttc
      241 catgcgggac cgggcgatga aatcctggac gaactgcttg tcggccagcg gcccgccgaa
      301 ggcctcgcga tcggcggcgc gcaggcacat catctccagc gcccgttcgg cggtcccgat
      361 cgcccgcatg cagtgatgga tgcgcccggg cccgagacgg tcctgggcga tcaggaagcc
      421 ggccccccgg gcaccgagca ggttctcttc cggaacccgg cagtcttcgt aaaggatctc
      481 gcagtggccg ggacccttgg agtggcccat caccggtacc ggccggacca ggttgaagcc
      541 gggtgcgtcc agtggcacga ggatcatcga ggcgttggcg taggggttgt cgctctcctc
      601 cgtgttgacc atggcgatcg cgaactcggc gccgaccgcc ccggaggtga accacttgtg
      661 cccgttgatg acccactcgc cgtcgacgag ttccgccttg gccctgagac cggtcgggtc
      721 cgagccggcg gtgtccggtt cggtcatcga gaagcagctg cggatctcgc catcggtcag
      781 cggcgccagg tacttcgcct tctgttcgtc gttgccgtgc tcaagcagga tctccatgtt
      841 tcccgtgtcc ggggcggcgc tgttgaaggc catcggggcg accagcgggc tgcggcccat
      901 ctcctcgcag aggaaggcgt actgccagtt gttgaggccg gctccgtaat gctcgtccgg
      961 caggaagagg ttccagagac cttccgattt ggccttctcg cggatctgct cgagttcgac
     1021 cggataggcg accccggcct tcacctcgtc gtcaatcgcc tggtgaagct ccgcttcgcg
     1081 cgggtagatg tgctcgtcca tgaaggaacg gacgcgctcg aggagccctt caacttcgga
     1141 accggcttcg aaatccatgg ccgggacgct actacccgcc gatctgttgc agcgtcaggc
     1201 gcggcggtac cgggtgaaga ggtcgccgtc ggcctcgagc agcccggtca actcgaatcg
     1261 ggcttccctt ggcaggttcg cttcgagaat gtgcgtggcg tcgccgccgg tggcgactgg
     1321 cgtcatggtc agaaagagct cgtcgacgag gtcgccggca accagctggc cgaatacacg
     1381 cgggccgcct tcgcaaagca ccgcgcgaac tccctcgttc cggcgcaggt aagcgagcac
     1441 ttcggccgag tcgatcaacc cgtcgatcgt caccagctcg accggtgtct ctgtttcggg
     1501 cagggacttc ttgcgcgaga aaatcaggac ccgcccctcg ccgttcgtga agagcggagc
     1561 atcgaaaggc agatcgaggt cgccgctgat gatcaccgcc agcgcgtcgc catcaagtcc
     1621 ggcggcttcg cggcgccggc ggtattccgg acgggagatg atccggccgt accgttccgc
     1681 ccgcatcgtg ccgccgccga tcaacaccgc gtcgaagcgg gtcctgaggc ccagcaggtg
     1741 ctcggtatcg gcgttggagc cgagc
//
LOCUS       DAOQFI010000478         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_43725_length_1765_cov_3.219298, whole genome shotgun sequence.
ACCESSION   DAOQFI010000478 DAOQFI010000000
VERSION     DAOQFI010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43725_length_1765_cov_3.219298"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLE93_08320"
     CDS             <1..547
                     /locus_tag="PLE93_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87093.1"
                     /translation="AFGTPAEEKRNHRWAAASPINFAGRTDPPIFFVVPTTSPDRWVD
                     AHVMARKLGQRLGLVSHRVKTEHAGVVPLLGNPSGDMGVTAPLMRFAHAAVNPVRYTP
                     TVRGTRIVRAGRDGYGTFRLKFSSKPAARALICQVDRGVQSVCPVAMKSPPGLHRLRV
                     WAYGNTGRAAGLTTVKLRVIR"
     gene            544..1407
                     /locus_tag="PLE93_08325"
     CDS             544..1407
                     /locus_tag="PLE93_08325"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNH87094.1"
                     /translation="MSRSIRVLLLVAVAGLFALSGIATQASARTYEPSFAAVSTDKGP
                     VFREGCLIYTKRATSPPCRYGEVGSSRKVTVLGDSHALQWTPPLINLARERGWELTML
                     LHANCTAALVNIDPYCNRWRHNALKRIRREKPTLVFVASNTAPSTFVMKNGRRLGRAA
                     SEPLFRQGMYETLLKLRKYGAQVTVMRDLPMSKAFLPSVCVSENVDQPGRCTFPARRP
                     LSQAYDFAAAKRLKAVQIIDPLPKVCPGGKCRAVHGNVLKYRDRGHISATYARLLTNW
                     LNGQLQNPFKA"
     gene            1484..>1765
                     /locus_tag="PLE93_08330"
     CDS             1484..>1765
                     /locus_tag="PLE93_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNH87095.1"
                     /translation="MAGLMLVAVPAAAEPARVITDINYNADEVGPAQSTSNSLDLYLP
                     ATDDGFEGLRPVVVWVHGGAWMTGDKSNRMTDKVNLFNSLGYIVASLNYR"
BASE COUNT          300 a          603 c          555 g          307 t
ORIGIN      
        1 cgctttcggg actcccgcgg aggaaaagag gaatcaccgt tgggcggccg cttcgccgat
       61 caacttcgcc ggccggactg atccgccgat ctttttcgtg gtcccgacca ccagcccgga
      121 ccgctgggtg gatgcccacg tgatggccag aaagctgggc cagcgactgg gcctcgtctc
      181 ccaccgggtc aagaccgaac atgccggcgt cgtgccgctg ctcggcaatc cgtccggtga
      241 catgggagtc accgcaccgc tgatgcggtt cgcccacgcg gcggtcaacc cggtccgcta
      301 caccccgacc gtccgcggta cgcggatcgt gagggccgga cgggacggat acggaacctt
      361 ccggctgaag ttcagctcga agccggccgc ccgggccctc atatgccagg tcgaccgcgg
      421 cgtccagtcg gtatgtccgg tcgcgatgaa gtcgccgccg ggtcttcacc ggttgcgggt
      481 ctgggcctat ggcaacaccg gtcgcgctgc cggtctgacg acggtcaagc tgcgggtgat
      541 ccggtgagcc gttcaatccg tgttctgctg cttgtggccg tggccggcct tttcgcgctt
      601 tccgggatcg ccacgcaggc ttcggcccgc acctacgaac cctcgtttgc cgcggtaagt
      661 accgacaagg gcccggtctt ccgcgagggt tgtctgatct acacaaagcg ggccacctcg
      721 ccgccctgcc gttacggcga ggtcggttcc agccgcaagg tgacggtcct cggcgactcg
      781 catgccctgc agtggactcc accgctgatc aacctcgcca gggaacgggg ctgggagctg
      841 accatgctgc ttcatgccaa ctgcactgcc gcattggtca atatcgatcc ctactgcaac
      901 cgctggcgtc acaacgcact gaagcggatc aggcgggaga agccgaccct ggttttcgtc
      961 gcgagcaaca ccgcccccag caccttcgtg atgaagaacg gcaggcggct gggccgggca
     1021 gccagcgaac cccttttccg gcaggggatg tacgagaccc tgctgaagct gcgcaagtac
     1081 ggagcgcagg tgacggtgat gcgtgacctg cccatgtcaa aggccttcct gccctcggtc
     1141 tgcgtttcag agaacgtcga ccagcccgga cgatgcacct tcccggcccg ccggcccctt
     1201 tcccaggcct acgatttcgc cgctgcgaaa cggctcaagg cggtgcagat catcgatccg
     1261 ctgcccaagg tctgccccgg aggcaagtgc cgtgccgtcc acgggaacgt cctcaagtac
     1321 cgcgaccgcg gccacatcag cgccacctac gcccgcctgc tgaccaactg gctgaacggt
     1381 cagcttcaga atccgttcaa ggcctgaggc cgctccgttg gccccggagc cgggttttcc
     1441 gggtatgacc ggtcggatca cagccgcaat ctgttcgctc gcgatggcgg gtctgatgct
     1501 ggtcgcggtt ccggccgcag ccgaaccggc aagggtaatc accgacatca actacaacgc
     1561 cgacgaggtg gggccggcgc aatcgacctc gaacagtctc gatctctacc tgccggccac
     1621 cgacgacggc ttcgaaggtc tgcgtccggt cgtggtctgg gttcacggcg gggcctggat
     1681 gaccggggac aagtcaaacc ggatgaccga caaggtgaac ctgttcaact cgctcggcta
     1741 catcgtggcc agcctcaact accgg
//
LOCUS       DAOQFI010000479         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_43939_length_1760_cov_4.116129, whole genome shotgun sequence.
ACCESSION   DAOQFI010000479 DAOQFI010000000
VERSION     DAOQFI010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43939_length_1760_cov_4.116129"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..635)
                     /locus_tag="PLE93_08335"
     CDS             complement(138..635)
                     /locus_tag="PLE93_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH87096.1"
                     /translation="MSELTEMHKTLVTTPSDTEIRTERIFDAPPEVIFDFWTDPELLA
                     QWVGPDRLEMTVEEYDVRPGGKYRYVHKDPESGAEYVFFGEFLEIEEPRKLSQTFNFV
                     MEPQPPPSIDRLELIPCEGGRTRLVTLTTFEAREYRDGMLQSGMEKGMDEGYAKLDRL
                     LADRT"
     gene            complement(632..994)
                     /locus_tag="PLE93_08340"
     CDS             complement(632..994)
                     /locus_tag="PLE93_08340"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH87097.1"
                     /translation="MVQYLDERAGSAERAFAALADPVRLAVLDRLGEGGASISELAGP
                     AGMTLTGMKKHIRILEEAGLVRTEKRGRTRWCELATEGFDEAAFWLDEFRRRRHQQLN
                     RLEEAIEEKKEQKKEQEK"
     gene            complement(1312..>1760)
                     /locus_tag="PLE93_08345"
     CDS             complement(1312..>1760)
                     /locus_tag="PLE93_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNH87098.1"
                     /translation="HPRNGGPAVALQLFAWVIQMLSCWALMFALGLESQAGLAAAAAV
                     LLAVNVTAIVPATPSNIGVFQLAVIFVLHKGWGVSTADALAYGVILQAVEMATAAALG
                     VPALVREGLTWSDLRTQAIASAPVELDPYSKNARRQEKRRQITYTR"
BASE COUNT          301 a          586 c          562 g          311 t
ORIGIN      
        1 gcccaaattc cccgaaagac tcgactggac ccaggttctc gggcaacagt aagcagagcc
       61 cctcccagcg attacttcct tcgggtggga ggggccggtt tccatgggag gtccggcgca
      121 gtccggaccg actgcagtca ggttcggtcg gcgagcagtc gatcgagctt cgcgtagccc
      181 tcgtccatgc ccttctccat gcccgactga agcatgccgt cgcggtactc cctagcctcg
      241 aatgtagtga gggtcaccag tctggtccgg ccgccttcgc aggggatcag ttcgagccgg
      301 tcgatcgaag gcggcggctg gggctccatc acgaaattga aggtctggga aagcttccgc
      361 ggctcctcga tctcgaggaa ctcgccgaag aagacgtatt ccgcgccgga ctccgggtcc
      421 ttgtggacgt accggtattt gccgcccggg cgcacgtcgt actcctccac cgtcatctcc
      481 agccgatcgg gccccaccca ctgggcgagc agttcgggat cggtccagaa gtcgaagatg
      541 acttccggtg gcgcgtcgaa gatccgctca gtccggatct cggtgtcgct cggggtggtg
      601 accagggtct tgtgcatttc ggtcaattca ctcatttctc ctgctccttc ttttgctctt
      661 tcttctcttc gatcgcttct tccagccggt tcagctgctg gtgacggcga cgacggaact
      721 cgtcgagcca gaacgcggcc tcgtcaaagc cttcggtcgc cagctcgcac cagcgggtcc
      781 ttccccgctt ctcggtccgg accagccctg cctcctcgag gatccggatg tgtttcttca
      841 tcccggtcag ggtcatcccg gctggcccgg caagctcgga aatggaggcc ccgccctcgc
      901 ccagccggtc gagaaccgcc agccgaacgg gatcggccag ggccgcaaaa gcccgctccg
      961 ccgaacccgc tcgctcatct agatactgaa ccatacggtt cactataaca caccccattc
     1021 ccaaagcccg attttcgcca aataccgcac ccaactccga cctcgtctca acccccaaag
     1081 accgagatcg cgtccgtcgg cgctatccgc cgacaaaccg catctcgcga aatgccaccg
     1141 ccacaaaccc cgagcgggtc gtgcacaggg acctccagcc gttcgctggc aaggagacag
     1201 gccggaaatg accgcgatag tacgcgggta catgagctgt cgtttccggc cgacgacgaa
     1261 gccagcggac ggctggtggt tcctgggcgc gttgcccggg ccccgggggc gttagcgggt
     1321 gtaagtgatc tgccgccgct tctcctggcg gcgcgcattc ttcgagtagg ggtcgagttc
     1381 gaccggggcg ctggcgatcg cctgggtccg gaggtcggac caggtgaggc cttcgcggac
     1441 cagggccggc actccgagcg cggcggcggt cgccatttca actgcctgga ggatcacgcc
     1501 gtaggcgagc gcgtcggcgg tcgagacgcc ccagcccttg tgcaggacga agatcacggc
     1561 gagctggaag acgccgatgt tcgacggggt ggccgggacg atcgcggtca cgttgacggc
     1621 gaggagcacg gccgcggcgg cggcgagccc ggcctggctt tcgaggccga gggcgaacat
     1681 cagggcccag caggagagca tctggatgac ccaggcgaag agctgaaggg caacggccgg
     1741 accgccgttg cgggggtgcc
//
LOCUS       DAOQFI010000480         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44064_length_1758_cov_2.715208, whole genome shotgun sequence.
ACCESSION   DAOQFI010000480 DAOQFI010000000
VERSION     DAOQFI010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44064_length_1758_cov_2.715208"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLE93_08350"
     CDS             <1..190
                     /locus_tag="PLE93_08350"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575172.1"
                     /note="catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNH87099.1"
                     /translation="STQTLGPILDGLMRNTPEALEFIDKAESEGVGRVIEDRDGGFGD
                     YSQAPPELQPDPRHIIEP"
     gene            190..1398
                     /locus_tag="PLE93_08355"
     CDS             190..1398
                     /locus_tag="PLE93_08355"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HNH87100.1"
                     /translation="MPASRARRNPDEHYGASNLELFFDLVFVFAITQVSHLLLENLTW
                     RGALEAAMVLLVVWWAWQYTTWATNELDTDRMPVRFVLLVIMMACLLMAVAIPEAFGD
                     KGLLFALSYVAIQIGRQAFLAFSAEPHTIARARGLHILIWFCFAAPFWIVGALVDGDA
                     RIAFWLVALTIDYCGPFVTYYVPWLKKVSPDAWKIGSGHFAERFQLFTIIALGETIVL
                     TGATTAKLEFDLVTALAFMAAFVSTACLWWLYFNYMATILERVLDEAEDRTAIGRDIF
                     TYGHIGIIVGIILCAVGDEIILTHPKEYLHTPELVAVVAGPALYLLAFVPTRWRLTHG
                     LPWRRVSGAIACIAIGVFAYLVHPPALVTGGLLLLALIGVISHEYFFRWQRSDAEELD
                     LARKLNVETR"
     gene            1465..>1758
                     /locus_tag="PLE93_08360"
     CDS             1465..>1758
                     /locus_tag="PLE93_08360"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNH87101.1"
                     /translation="MPEPVERRSGQRYMPGLDGLRAIAVLAVIAYHLRVGFAPGGLLG
                     VGVFFTLSGYLITDILLEGWVTRKLSLKNFWLARARRLLPALFTMLAVVLLWVW"
BASE COUNT          260 a          597 c          555 g          346 t
ORIGIN      
        1 ctcgacccag accctcgggc cgattctcga cgggctgatg cggaacacgc cggaagcgct
       61 ggagttcatc gacaaggcgg aatccgaagg ggtggggcgg gtgatcgagg atcgcgacgg
      121 tggcttcggc gactactcgc aggcgccgcc ggagctccag cccgatcccc gccacataat
      181 cgagccctga tgccggcaag ccgcgcccgc cgcaacccgg acgagcatta cggggcatcg
      241 aaccttgagc tcttcttcga tctcgtcttc gtcttcgcga tcacccaggt ttcgcatctg
      301 ctgctggaaa acctgacctg gcggggtgcc ctcgaggcgg cgatggtgct gctcgtggtc
      361 tggtgggcct ggcagtacac gacctgggcc accaacgagc ttgacaccga ccggatgccg
      421 gtccggttcg ttctgctggt gatcatgatg gcctgcctgc tgatggcggt cgccatcccg
      481 gaggccttcg gcgacaaggg cctgctcttc gccctctcct atgtggcgat ccagatcggc
      541 cggcaggcat tccttgcctt cagcgccgaa ccgcacacga tcgcccgggc ccggggcctt
      601 cacatcctga tctggttctg ctttgccgcc ccgttctgga tcgtcggcgc gctggtcgat
      661 ggcgacgccc ggatcgcgtt ctggctggtc gccctgacga tcgactactg cggaccgttc
      721 gtgacctact acgtcccctg gctcaagaaa gtgagccccg acgcctggaa gatcggcagc
      781 ggacacttcg ccgagcggtt ccagctgttc acgatcatcg ccctggggga gacgatcgtt
      841 ctcaccggag cgaccacggc gaaactcgag ttcgacctgg tcacggccct cgcgttcatg
      901 gccgccttcg tcagtaccgc ctgcctgtgg tggctctact tcaactacat ggcgaccatc
      961 ctcgaacggg tcctcgacga ggcggaagac cgcaccgcga tcggtcgtga catcttcacc
     1021 tacggccaca tcggaatcat cgtcgggatc attctctgcg cggtcggtga cgagatcatc
     1081 ctcacccatc cgaaggagta cctccacaca ccggaactgg tcgccgtggt tgcgggcccg
     1141 gccctttacc tgctggcctt cgtgccgacc cggtggcggc tcacccacgg gcttccgtgg
     1201 cggcgggttt ccggcgcaat cgcctgcatc gcgatcgggg tctttgccta cctcgtgcat
     1261 ccgcccgccc tggtaaccgg tggcctgctt ctgctcgccc tgatcggggt gatcagccac
     1321 gagtacttct tccgctggca acgaagcgat gccgaggagc ttgacctggc gcgcaaactg
     1381 aacgtcgaga cccggtgaca gctgcctgac cggcgccacc ccgggatggt ggggtggctc
     1441 gctggcgggt agattctccg ggtcatgccg gaaccagtag aaaggcgatc cggtcagcgc
     1501 tacatgcctg gcctggacgg gctcagggcc attgccgttc tcgcggtgat cgcctaccac
     1561 ctccgggtcg gattcgcgcc gggtggcctg ctcggggttg gcgtgttctt cacgctttcc
     1621 ggctacctga tcaccgacat cctgctcgag ggctgggtga cccggaagct gagcctgaag
     1681 aacttctggc tggcccgcgc ccgccggctc ctgccggccc tgttcacgat gctggccgtc
     1741 gtcctgctct gggtctgg
//
LOCUS       DAOQFI010000481         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44239_length_1755_cov_2.022353, whole genome shotgun sequence.
ACCESSION   DAOQFI010000481 DAOQFI010000000
VERSION     DAOQFI010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44239_length_1755_cov_2.022353"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLE93_08365"
     CDS             <1..702
                     /locus_tag="PLE93_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87102.1"
                     /translation="AEIVDPDFFTKIRHGLSDPEVALVGCAGATGVRSIAWWEGAVTW
                     ASYTHRYQEFGGGEIPAICWVYEDTPSFAGTGEVDSIDGFVIAFSPWAIHNLRFDETI
                     GGKLHGYDFDISMQVKEAGKKIVTEDLRVIHHHSLELISEMEGWVDTHMQLSRKWQHL
                     LSDNGGGEPDWEERARRAEAELSATRLMAGAGEIIWEARTQELERELNEVKNSTSWKI
                     TRPLRRLRGSREKNQ"
     gene            983..>1755
                     /locus_tag="PLE93_08370"
     CDS             983..>1755
                     /locus_tag="PLE93_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87103.1"
                     /translation="MDEDESPVVLSGFTDETDGSACSGFGVSRFDSSGVPDPSFGVGG
                     LVVSDSECEPRHTLAALPGGSFVLAGPQRYGSACLEATKLLSDGSVFEGWNQGGNNGA
                     SRFCYAPAAGPAGVTALASEADDKGRVLIAGSAQWGRKHLFGTVRRIRPNGTTDSSFR
                     GTGKGWAGEPGLVRLFETGPRHYNVVNAVTTLASGKILAGGTNRGGFAIARLNPKGSA
                     DESFGSASGVARIDPDGRKCEAEGFAGSPCVASTAQDMIV"
BASE COUNT          333 a          500 c          587 g          335 t
ORIGIN      
        1 gccgaaatcg tcgatcccga cttctttacg aagatccgcc acgggctgag tgaccccgag
       61 gtcgctctgg tcggctgcgc cggtgcgacc ggggtccggt cgatcgcctg gtgggagggt
      121 gcggtcacct gggcctccta cacccaccgc taccaggagt tcggcggcgg cgagatcccg
      181 gcgatctgct gggtgtacga agacacgcct tccttcgccg gcaccggtga ggtcgattca
      241 atcgacggtt tcgtgatcgc cttctcaccc tgggccattc acaacctccg cttcgacgag
      301 acgatcggcg gcaagcttca cggttacgac ttcgacatct cgatgcaggt aaaggaagcc
      361 ggcaagaaga tcgtcaccga ggacctgcgg gtcattcacc accactcact cgagttgatc
      421 tccgagatgg agggctgggt ggacacccac atgcagcttt cgcgcaagtg gcagcacctg
      481 ctctccgaca atggtggcgg cgagcccgac tgggaggagc gagcccgccg ggccgaggcc
      541 gagctttccg cgacccgcct gatggccggc gccggagaga tcatctggga ggcccgtacc
      601 caggaactcg aacgggaact gaacgaggtc aagaacagca cctcgtggaa gatcacccgc
      661 cccctccgcc gcctccgcgg cagccgcgaa aagaaccagt aaccgaaccg ggctggacgc
      721 tacggccagc gaaggcgcga cttgcttcgt cgctacgggt tagcttggaa ggggtcacgg
      781 aatgatggag acttggcatg agttacgagg tcgcggccgg gctgtcgcct gtgggttcat
      841 agcgggcgga ctctcggcgc tcggtttctt tgcgctttgc tcagcagagg ccgtcgcagc
      901 ggcgaagctt gatccgtcgt tcggagtcgc gggccgggta gttgtgagtg atccaaggct
      961 tcatcccgca atcgaaatgg ctatggatga ggacgaatct ccggtggtgc tttccggctt
     1021 caccgatgag accgatgggt cggcatgctc tggctttggg gtatccaggt tcgattcttc
     1081 aggagttccg gacccgtctt tcggggttgg cggcctcgtg gtttctgact cggaatgtga
     1141 accgcgacat acgttggccg cgctaccagg gggcagcttt gtactggccg ggccgcaaag
     1201 gtacggcagc gcctgtcttg aagccacaaa actgctctct gacggttcag tattcgaggg
     1261 gtggaatcag gggggaaaca atggcgccag ccggttttgt tatgcgcccg ctgctggtcc
     1321 agctggcgtc acggcgcttg cgagtgaggc agatgacaag ggacgagtgc tgatcgcagg
     1381 atcagcgcag tggggaagga agcatctgtt cggcaccgtt cgacgaattc ggcccaacgg
     1441 gacaacagat tcatcatttc gcggaactgg aaaaggctgg gccggagaac ctggattggt
     1501 caggctgttc gaaacgggac cccgccacta caacgttgta aatgcagtga cgactttggc
     1561 ttccggcaag attctggccg gcggaaccaa ccggggaggg ttcgccatcg cgaggcttaa
     1621 tcccaaaggg tccgctgacg agtcgtttgg gtcagcatcc ggggtagcgc ggattgatcc
     1681 cgatggacgc aaatgtgagg cagaaggatt cgcgggcagt ccgtgcgtgg cttcgaccgc
     1741 gcaggacatg atcgt
//
LOCUS       DAOQFI010000482         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44246_length_1755_cov_1.468235, whole genome shotgun sequence.
ACCESSION   DAOQFI010000482 DAOQFI010000000
VERSION     DAOQFI010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44246_length_1755_cov_1.468235"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="PLE93_08375"
     CDS             complement(<1..1696)
                     /locus_tag="PLE93_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87104.1"
                     /translation="MASYITIPDDPTLRQLIFRKFSDTYQDAELKRIRGGEWPRAGLE
                     IVRNKKTRMIPFTIKVPGISTASESFEEGFNAPVMDKVVTVMASAPIPAEVQFVATPV
                     PRPFDRLIYHLSRFKLASSQRADAIERSEGENTANASANEGHVFMEMRVGSPNYAFSR
                     DIAAVLQGAAGGDAQIRERRPVLRKKIYQERFYKAVGNPLPSWLYSVYSTSELASIWQ
                     LPSAQLRGVATKRHNRRLVSVPPEVHYIDPDAPPPTKDTQVEDMAMWLSPERREISLI
                     NGDFLFGVGVAGQQGMGKTVLMGRMVSALLSDDRSFSGLICDPKGDLAEAVLSYLPPD
                     RKVHYIDFDDPQIGINPFEITDMADDEAIVNVILHGIIDVARTEEDESQIMASSKDFL
                     NMALHATLATTVPFGYKPTFNHLRKWISSDPHNVAWRQSLITTVLSKRRSKLWVCEGY
                     EEYHSSLKRSEAQFTTRAAAPMNKIAELLNESIAKVLRHDQAINIETAIRNNEIIIVN
                     GRNYTQADIIFRFLWQMVDQTLGRLETVRNKALRDREMGEDVKVPDPVKFLFGVDEA"
BASE COUNT          326 a          486 c          547 g          396 t
ORIGIN      
        1 gggcctcgtc aaccccgaat aggaacttga ccggatccgg aactttcacg tcctcgccca
       61 tctcccggtc tcgaagcgcc ttgtttcgaa cggtttccag ccgccccagg gtttggtcga
      121 ccatctgcca aaggaaccgg aaaataatgt cggcctgcgt gtagttgcgg ccgttgacaa
      181 tgatgatctc gttgtttctg atcgcggttt caatgttgat cgcctggtcg tggcgaagaa
      241 ccttggcgat cgactcgttg agcagctcgg cgatcttgtt catcggggcc gctgcccggg
      301 tcgtgaactg cgcctccgag cgtttgagcg aggagtggta ttcctcgtag ccctcgcaaa
      361 cccacaactt ggatctgcgt ttggaaagaa ccgtggtgat gagcgactga cgccaggcga
      421 cgttgtgcgg gtcagacgag atccacttgc gaaggtgatt gaaggttggc ttgtagccga
      481 aaggcaccgt tgttgccaga gtcgcgtgaa gcgccatgtt caggaagtcc ttggaggaag
      541 ccatgatctg cgattcgtct tcctcggtgc gggcaacgtc aatgattccg tgaaggatca
      601 cgttgacgat cgcctcgtcg tctgccatgt cggtgatctc gaacgggttg atgccgatct
      661 gcggatcgtc aaagtcgatg tagtggactt tccgatcggg aggcagatag gagagaaccg
      721 cttctgcgag gtcacctttc gggtcacaga tcaggccgga gaaactccgg tcgtcggaaa
      781 gaagtgccga aaccatgcgg cccatcagca cggttttccc catgccttgc tggccggcaa
      841 cgccgacacc gaagaggaag tcgccgttga tcagggagat ctcgcggcgt tcaggtgaaa
      901 gccacatcgc catgtcctcg acctgggtgt ctttggtcgg tggcggtgcg tccgggtcga
      961 tgtagtgaac ctccggtgga accgatacga gccggcggtt gtgccgtttg gtggcgaccc
     1021 ctcgaagttg tgctgagggc agctgccaga tgctcgccag ctcgctggtc gagtacaccg
     1081 agtaaagcca gctcggcagt ggattgccaa ccgccttgta gaagcgctcc tgataaatct
     1141 tcttgcggag caccggtcgg cgttcacgga tctgcgcgtc accgccagcc gcgccttgaa
     1201 gcaccgctgc tatgtcgcgg ctgaacgcgt agttcgggga gccaacccgc atttccatga
     1261 acacatgccc ttcgttggct gatgcattgg cggtgttttc accttcggat ctttcgattg
     1321 cgtcggcccg ctggcttgag gcgagcttga atcggctgag gtgatagatc agccggtcaa
     1381 atggcctcgg gacaggggtg gcaacgaact ggacctcggc tgggatcggg gccgatgcca
     1441 tcacggtcac gaccttgtcc atgaccggcg cgttgaaacc ctcttcaaag gactcgctgg
     1501 cagtgctgat tcctggcact ttgatggtga acgggatcat ccgggtcttc ttgttgcgga
     1561 cgatttcgag accggccctc ggccattcgc ctccacggat tcttttcagt tccgcgtcct
     1621 gataggtgtc ggaaaacttc cggaagatca gctggcggag ggttgggtcg tcaggaatgg
     1681 tgatgtagct ggccacttca ctgtccccgc gaatcctgtt cggcacagac agcaccctga
     1741 acgccattga cgggc
//
LOCUS       DAOQFI010000483         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44276_length_1754_cov_2.692760, whole genome shotgun sequence.
ACCESSION   DAOQFI010000483 DAOQFI010000000
VERSION     DAOQFI010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44276_length_1754_cov_2.692760"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLE93_08380"
     CDS             <1..719
                     /locus_tag="PLE93_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH87105.1"
                     /translation="SARAPEPQPELARLAFDDQQTQDHAVVGIFLASIISVALAWTIF
                     KLAAVFRGVVGAGLPRKLSLPFDPDRDHFLGSPDAPFVLVEYGDYECPFCGRATGMVR
                     ELRERMGDQLLYVYRHLALVDVHPHAELAAEAAEAAGEQGKFWEMHVELYDHQGDLDI
                     EDLIGYAAKLDMDVDRFVEDVQAGRLARRVQQDAASGDASGAHGTPTFFVNGVRHVGA
                     YDAVSLAAELRAAEPVPAGA"
     gene            complement(722..1738)
                     /locus_tag="PLE93_08385"
     CDS             complement(722..1738)
                     /locus_tag="PLE93_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87106.1"
                     /translation="MAVMRGQRGLGLLVVGAILASALVLLPATSPAATKKQKQCQRAI
                     ATVFTFKKGVPKKKRRKAVKIICQTMTVGKQGPKGAKGDPGARGATGATGPKGATGSA
                     NGVTGPVGPTGATGQKGATGAIGLPGITGATGATGFQGLIGPTGPIGLPGLTGATGAT
                     GLIGEIGPTGPTGLTGLIGPTGPTGLTGAVPILISDIPSFSAQAMSDVINPITNWNED
                     YDSTNSFNPVTGVFTAPTTGSYLIEPDITTGPASEVVAGGGQPPLVAVNVNNVDVDVQ
                     AFPLFNVDINLILALDVPLQTAQASPTTFQQLDAGDQVSIQIIKQNGVVYDTYGDLMI
                     TRLP"
BASE COUNT          260 a          610 c          567 g          317 t
ORIGIN      
        1 tctcagctcg tgcgccagag ccgcagccgg aactcgcccg gctcgccttc gatgaccagc
       61 agacccagga tcatgcggtg gtcggcatct tcctcgcctc gatcatctcc gtcgccctgg
      121 cctggacgat cttcaagctc gcggcggtct tccgcggcgt ggtcggcgcc ggcctgcccc
      181 gcaagctcag cctgcccttc gatccggatc gcgaccactt cctcggcagt cccgacgcgc
      241 cctttgtgct ggtcgagtac ggcgattacg aatgtccatt ctgcggccgg gcaaccggga
      301 tggtccggga acttcgcgag cggatgggcg accagctgct ctacgtttac cgccacctgg
      361 cgctggttga cgtgcacccg cacgccgaac tcgcggccga ggcagccgag gccgccgggg
      421 agcaggggaa gttctgggaa atgcatgtcg agctctacga ccatcagggc gacctcgaca
      481 tcgaagacct gatcggatac gccgcgaagc tggacatgga cgtcgaccgc ttcgtcgagg
      541 atgtccaggc cgggcgcctc gcccgtcggg tccagcagga cgccgcctct ggtgacgcct
      601 cgggtgccca cggcaccccg accttcttcg tcaacggggt ccgtcacgtc ggcgcgtatg
      661 acgcggtcag cctcgccgcg gagctccgcg ccgccgaacc cgttccggcc ggggcctgaa
      721 gttacggcag cctggtgatc atcaggtcgc cgtaggtgtc gtacaccacg ccgttctgct
      781 tgatgatctg gatcgagacc tgatcccccg cgtcgagctg ctggaaggtg gtcggcgatg
      841 cctgtgctgt ctggagggga acgtccaggg cgagaatcag gttgatgtcg acgttgaaca
      901 gggggaacgc ctgcacgtcc acgtcgacgt tgttcacgtt gacggcaacc aggggtggct
      961 ggccgccgcc ggcaaccacc tcggacgccg gtccggtcgt gatgtccggt tcgatcaggt
     1021 acgagccggt cgtcggggcg gtaaacaccc ccgtgacagg gttgaaggaa ttcgtcgagt
     1081 cgtaatcctc gttccagttg gtgatcgggt tgatcacgtc ggacatcgcc tgggccgaga
     1141 aagacgggat gtccgagatg aggatcggca cggcgcccgt caggccggtc ggcccggtcg
     1201 gcccgatgag accggtcagt ccggtgggcc cggtcggccc gatctcaccg atcaaaccag
     1261 tcgcgccggt tgccccggtc agtccgggca gcccgatcgg tccggtcggc ccgatcaatc
     1321 cctgaaagcc ggtggcgccg gtcgccccgg tgattcccgg caaaccgatc gccccggtcg
     1381 cacccttctg acccgtcgct ccggtcggcc cgacgggtcc ggtcacaccg ttcgccgatc
     1441 cggttgcccc tttaggcccg gtcgctccgg tggcgcccct ggccccggga tcacccttcg
     1501 cgcccttcgg tccctgcttg ccgaccgtca tcgtctggca gatgatcttc acggccttcc
     1561 ggcgcttctt cttcggcacg cccttcttga aggtgaagac ggtcgcgatc gcccgctggc
     1621 attgcttctg tttcttggtc gccgccgggg aggttgcggg caagaggacc agcgccgagg
     1681 cgagaatcgc tccgaccacc aacagaccca gtccccgctg tccccgcatc acggccacgc
     1741 atccactcta catc
//
LOCUS       DAOQFI010000484         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44444_length_1750_cov_2.814159, whole genome shotgun sequence.
ACCESSION   DAOQFI010000484 DAOQFI010000000
VERSION     DAOQFI010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44444_length_1750_cov_2.814159"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PLE93_08390"
     CDS             complement(<1..1202)
                     /locus_tag="PLE93_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNH87107.1"
                     /translation="MIAIAGFALSCFALLLFVWVAFGGPTPLAARGYEVKLPLTQVGQ
                     LAEQSEVDISGVEIGRVKSIDLGEGDEEGSAIVTLNLEPEYAPIPKDTRAILRAKSLL
                     GEAYIELTPGDKRDGMLDDGGELPPAQVAKSVQLDEIFRTFDEKTRQAFMQGAIDNSI
                     AISGRGATLNQTLGILPGTVTSLTDVLQVLNEQDQDVSKLIKNTGVVFDALSKRQGEL
                     SGMIRNTDTVFKTLADRDQELKDFFRVFPTFLRESRATQERLGDFSDFATPIVRKLVP
                     VAKQLSPTFKASANLAPVSERLYTNLKPVIRKAPRAFPSLRAFLDEDAPTLLGRLPDY
                     LAEFNPFLETGSLYRKEIAGLIGNAASATQPSISGGAHYVRAGVRLGPETYTSYPGTG
                     PSNGRVNP"
     gene            complement(1229..>1750)
                     /locus_tag="PLE93_08395"
     CDS             complement(1229..>1750)
                     /locus_tag="PLE93_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87108.1"
                     /translation="DAGKVNLAKSDRLSQLTRPTLLGLIPGARNLKSSLQSVQRLAID
                     TTPLVRDDIRPFTREVQPVFKELAKTSQAGEKTTPKLQATFTQLNTLLNTLAYNPPGD
                     SEGFLFYAPWASHNFNASVAFQGGLGLVRRSVNMLTCSTSQSAAAVFGANVQLETAYR
                     LNQLPPPTAICTP"
BASE COUNT          292 a          519 c          609 g          330 t
ORIGIN      
        1 tacgggttga cccggccgtt cgatgggccg gtccccgggt aagaggtgta ggtctccggc
       61 ccaagacgga cgccggcccg gacgtagtgc gcaccgccgg aaatggaggg ctgggttgcc
      121 gatgctgcgt tgccgatcag tcccgcgatt tccttgcggt agagactgcc ggtttcaagg
      181 aacggattga actcggccag gtagtcgggc agcctgccga gcagcgtcgg ggcatcctcg
      241 tcgaggaagg cccttagcga cgggaacgcc cgcggcgcct tgcggatcac cggcttcagg
      301 ttcgtgtaga gccgttcact gaccggagcc aggttggccg aggccttgaa ggtaggcgaa
      361 agctgcttgg cgacaggtac cagtttgcgg acgatcgggg tggcaaaatc tgagaagtcg
      421 ccgagccgct cctgggtggc ccgggactcg cgcaggaagg tcgggaagac ccggaagaag
      481 tccttcaatt cctggtcccg gtcggccagg gtcttgaaga cggtgtcggt gttgcgaatc
      541 atgccgctga gctcgccttg ccgtttgctc agcgcatcga agaccacgcc ggtgttcttg
      601 atcagcttcg acacatcctg gtcctgttcg ttcaggacct ggagcacgtc ggtcagcgag
      661 gtcaccgtgc ccggcaggat gccgagcgtc tggttcaggg tcgcgccgcg cccgctgatc
      721 gcgatcgagt tgtcgatcgc tccctgcatg aatgcctgcc tggtcttctc gtcaaatgtg
      781 cggaaaatct cgtcgagctg caccgacttt gcgacctggg ccggcggcag ttcgccaccg
      841 tcatcgagca tgccgtcccg cttgtcgcct ggggtcaatt caatgtaggc ctcgccgagc
      901 agactcttgg cccgcaggat cgcccttgtg tccttcggaa tcggcgcgta ttccggctcc
      961 aggttcaggg tcacaatcgc cgagccctct tcatcgcctt caccgaggtc gatcgacttg
     1021 acccggccaa tctcgactcc ggagatgtcg acctcggact gctcggccag ctggccgacc
     1081 tgggtcagcg gcagcttgac ttcatagccc cgtgccgcca gaggtgtcgg gccgccgaag
     1141 gccacccaga cgaaaaggag cagcgcgaaa cacgagagcg caaagcccgc gatggcgatc
     1201 atctgtccga ctgaaggggc ctgtttgttc atggggtgca gattgcggtc gggggcggca
     1261 actggttcag tcggtaggca gtctcaagct ggacgttggc cccgaagacg gccgcagccg
     1321 actgggaggt cgaacaggtc aacatgttga cgctgcggcg aaccaggccg aggccgccct
     1381 ggaaggccac cgatgcgttg aagttgtggc tggcccaggg cgcgtaaaag aggaagccct
     1441 cggagtcacc gggcgggttg taggcgagcg tgttgagcag cgtgttcagc tgggtgaagg
     1501 tggcctgcag cttcggggtg gtcttctccc ctgcctggct cgtcttggcc agctccttga
     1561 agaccggctg gacctcgcgg gtgaagggcc ggatgtcatc ccggaccagc ggagtcgtgt
     1621 cgattgccag gcgctggacc gactggagcg aggacttgag gttccgggcg ccggggatca
     1681 acccgagcag ggtcggcctg gtcagctgcg aaaggcggtc ggacttggcc aggttgacct
     1741 tgccggcgtc
//
LOCUS       DAOQFI010000485         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44489_length_1749_cov_3.397285, whole genome shotgun sequence.
ACCESSION   DAOQFI010000485 DAOQFI010000000
VERSION     DAOQFI010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44489_length_1749_cov_3.397285"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLE93_08400"
     CDS             complement(<1..553)
                     /locus_tag="PLE93_08400"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HNH87109.1"
                     /translation="MNPEIALSPNAVDVQSSQPATRVSLSRVGVTGVEKVISIGEDGH
                     YHAELECYVDLSPDQAGAHMSRFEENVSEAIEEVVRSQSMRAEQLAARIAELVRVKQK
                     GLRAEVSINARYAQTMPAPVSGIENQEIYNLLGTAVASPEGTRTVTGVQAQGMTACPC
                     AQGMVADLARTRLRSKGYDEDQVE"
     gene            673..>1749
                     /locus_tag="PLE93_08405"
     CDS             673..>1749
                     /locus_tag="PLE93_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH87110.1"
                     /translation="MEFETDELKALAASGRDRGYLTFDAVAEAVAELDLGKGDVEELN
                     SWLTEQGIDLISQEESELRGGDGDGRAPERNGSPVLDGTGGLDGSGPIESEQIPVVTN
                     GATVAAGEAGSDATLDSLRLYLRSIGQVDLLTAQQEVELAKRIERGDMEAKRQMVEAN
                     LRLVVSIAKGYLGRGLSFLDLIQEGSLGLIRAVEKFDYRRGYKFSTYATWWIRQAVTR
                     AVADKSRSIRIPVHMVEKLNRVHFVERQLVQDLGREPEPQEIAAELGWTVQEVRDVWR
                     VSQTPVSLEKPVGEGEETELVDLVKDENQMEPFDEASLNLRSEGIRRVLNSLPERERE
                     VLIMRYGLGGLNPRTLEECGEAFGV"
BASE COUNT          310 a          549 c          584 g          306 t
ORIGIN      
        1 cctcgacctg gtcctcgtcg taacccttgc tccggagccg ggtccgggcc aggtcggcga
       61 ccatcccctg ggcgcagggg caggcggtca tgccctgcgc ctgcactccg gtcaccgtgc
      121 gggtcccttc cggtgaagcg accgcggtgc cgagcaggtt gtagatctcc tggttttcga
      181 tcccggagac cggggccggc atggtctgcg cgtagcgggc attgatcgaa acctcggccc
      241 tgaggccctt ctgcttgacc cgcaccagtt cggcgatccg ggcggcaagc tgctcggccc
      301 gcatcgactg gctgcggaca acctcctcga tcgcctcgga gacgttctcc tcgaaccggg
      361 acatatgcgc cccggcctgg tcggggctca ggtccacgta gcactcgagt tcagcgtggt
      421 agtgaccgtc ttcaccgatg cttatgacct tctcgacacc ggtcactccg acccgggaaa
      481 ggctcacccg ggtggcgggc tgggaggact gaacgtccac ggcattgggg gaaagcgcga
      541 tttcgggatt cattttcctg agaacagcct agtaaccaga gtgagcggcc ggttaagaac
      601 actccgtata attccgcaac cgcgcacgaa ctcagggttt ccattagtgg aaattcgtta
      661 ctgggggtag ttatggagtt cgaaaccgac gaattgaagg cactggccgc ttctggcagg
      721 gatcgcggct acctgacctt cgatgccgtc gcggaagcgg ttgcggagct tgatctgggc
      781 aagggtgacg tcgaggaact caattcctgg ctgaccgaac agggcatcga cctgatcagc
      841 caggaggaat ccgagctgcg gggcggcgac ggggacggcc gggctccgga gcgaaacgga
      901 tcgcccgtgc ttgacggcac cggcgggctg gacggctcgg gcccgatcga atcggagcag
      961 atcccggtcg taaccaacgg cgccaccgtc gcggcgggtg aagccggttc cgacgccacc
     1021 ctcgattcgc tccgcctcta cctgcggtcg atcggccagg tcgatctgct caccgcccag
     1081 caggaggtcg agctggccaa gcggatcgag cgcggtgaca tggaggcgaa gcggcagatg
     1141 gtcgaagcca acctgcgcct ggtcgtctcg atcgccaagg gttacctcgg ccgcggcctc
     1201 agcttcctcg acctgatcca ggaaggctcg ctcggcctga tccgcgcggt cgagaagttc
     1261 gactatcgcc gcggttacaa gttttcgacc tacgcgacct ggtggatccg ccaggcggtg
     1321 acccgcgcgg ttgccgacaa gtcccgttcg atccgcatcc ccgtccacat ggtcgagaag
     1381 ttgaaccggg tccacttcgt cgagcgccag ctggtgcagg atctcggccg ggagccggaa
     1441 ccacaggaga tcgcggccga actcggctgg acggtgcagg aggtccgcga tgtctggcgc
     1501 gtctcgcaga ccccggtctc gcttgagaag ccggttggcg agggcgagga gacggaactg
     1561 gttgacctgg tcaaggacga aaaccagatg gagcccttcg acgaagcctc actcaacctc
     1621 cgcagcgagg gaattcgcag ggtgctgaac agcctgccgg aacgcgaacg cgaggtgttg
     1681 atcatgcgtt acggcctcgg cggcctgaat ccccgcaccc tggaggaatg cggcgaagcc
     1741 tttggcgtc
//
LOCUS       DAOQFI010000486         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44494_length_1749_cov_2.936836, whole genome shotgun sequence.
ACCESSION   DAOQFI010000486 DAOQFI010000000
VERSION     DAOQFI010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44494_length_1749_cov_2.936836"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..447
                     /gene="atpE"
                     /locus_tag="PLE93_08410"
     CDS             178..447
                     /gene="atpE"
                     /locus_tag="PLE93_08410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNH87111.1"
                     /translation="MDPSSIVPLAEVTGDVTDAGKAIALGVGAGLGSIGAGIGIGFIF
                     GKEIESVARQPELASELQSIRWLGFALTEAVAFYTFIFALIAFFL"
     gene            455..1045
                     /gene="atpF"
                     /locus_tag="PLE93_08415"
     CDS             455..1045
                     /gene="atpF"
                     /locus_tag="PLE93_08415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNH87112.1"
                     /translation="MEPVLSHIATVLPLAAEAAEEEGGSFLVTPGLGLMIWTLVLFGF
                     TFWVLKKFAFPLIGEALDKRAATIRESIEAAEKQKEESDKLLADYRQRLTEAREQADE
                     IVTKARKAAETTKSEASAEGQAKREELVDAARKDIEAEKTKALDDIRKEVANLTVLAT
                     ERVTRKSLDDAAHKELVEDALREVDFSALAGEERNN"
     gene            1045..1578
                     /gene="atpH"
                     /locus_tag="PLE93_08420"
     CDS             1045..1578
                     /gene="atpH"
                     /locus_tag="PLE93_08420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNH87113.1"
                     /translation="MEELARVYAESLFGVAREKGNLDEVQAELAQFSDALEGNRELAV
                     FFFSPYFSSKEKLDGLNKAVEGTTPEFRNFLELLIEKHRMPAIFRIRRDFDDLWKKEN
                     DRIEVTVTSAVELDPAVSREVADAVEKQTGKQVDLTSRVDEGIIGGLVLQVGNMVLDS
                     SIRNNLEKLRQSVAQAA"
BASE COUNT          354 a          554 c          539 g          302 t
ORIGIN      
        1 gcagtcctgc tcggtatcgc cgctctcggc gtcctgacct acccgatggc tttcgccttc
       61 tacgtgttcg aggtcggcat cgtggccacc ctgcaggcat tcatcttttc catcctgacc
      121 gctatctaca tcggcggagc aaccgccgaa agtcactaac caaaaggagt cccagtaatg
      181 gatcccagtt ccatcgtgcc cctcgccgaa gtcaccggtg acgtcaccga cgccggcaag
      241 gcgatcgctc tcggcgtagg cgccggactc ggttcgatcg gcgccggcat cggcatcggc
      301 ttcatcttcg gcaaggagat cgagtcggtt gcccgtcagc ccgagctcgc ttccgagctc
      361 cagtcgatcc gctggctcgg cttcgctctg accgaggccg tcgccttcta caccttcatc
      421 ttcgcgctga tcgcgttctt cctgtaggcc gaccgtggag cctgttctct cacatatcgc
      481 gaccgtgctg cctctggcag ccgaagcggc cgaggaagag ggcggcagct tcctggtgac
      541 gccggggctg ggtctgatga tctggaccct ggtgctcttc ggcttcacct tctgggtgct
      601 gaagaagttc gccttcccgc tgatcggcga agccctcgac aagcgcgcgg caacgatccg
      661 cgagagcatc gaggctgccg aaaagcagaa ggaagagtcg gacaagctgc tggccgatta
      721 ccgtcagcga ctcaccgaag cccgtgagca ggccgacgaa atcgtcacca aggcccgcaa
      781 ggccgccgag acgaccaagt ccgaagcaag cgcggaaggg caggccaagc gcgaagagct
      841 ggtcgacgcc gcccgcaagg acatcgaggc cgagaagacc aaggccctgg acgacattcg
      901 caaggaagtc gccaacctga ccgtcctcgc gaccgagcgg gtcacccgca agtcgctcga
      961 cgatgccgcc cacaaggaac tggtcgagga cgccctgcgc gaagtcgact tctccgccct
     1021 ggccggcgag gaacggaaca actgatggaa gaattggctc gggtatatgc cgagtccctc
     1081 tttggcgtag ccagggagaa gggcaacctg gacgaagtgc aggccgagct tgcacagttc
     1141 agcgatgccc tggaaggaaa ccgcgagctc gcggtcttct tcttcagtcc ctacttctcc
     1201 tcgaaggaga agctcgacgg cctgaacaag gcggtcgagg ggaccacgcc cgaattccgc
     1261 aacttcctcg agttgctcat cgaaaagcac cggatgccgg cgattttccg gatccggcgc
     1321 gatttcgatg acctctggaa gaaggaaaac gaccggatcg aagtgaccgt gaccagtgcg
     1381 gtcgagctcg atccggccgt gtcccgggaa gtcgccgacg cggttgaaaa gcagaccggc
     1441 aagcaggtcg atctgaccag ccgcgtcgat gaagggatca tcggcggact cgtacttcag
     1501 gtcgggaaca tggttctcga cagcagcatt cgcaacaact tggagaaact ccgccagagc
     1561 gtggctcagg cggcctaagg aggatttgga ccgagatgca gatcaagcca gacgagattg
     1621 ccagcattct gcggcagcgc atcgagggca tcgaccccgg tagcgccgac ctgaccgagg
     1681 tcggcaccgt gctctcggtc gctgacggta tcgcccgtat tcacgggctc gacaactgca
     1741 tggcgctgg
//
LOCUS       DAOQFI010000487         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44713_length_1744_cov_3.676140, whole genome shotgun sequence.
ACCESSION   DAOQFI010000487 DAOQFI010000000
VERSION     DAOQFI010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44713_length_1744_cov_3.676140"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLE93_08425"
     CDS             <1..639
                     /locus_tag="PLE93_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNH87114.1"
                     /translation="TVSTYMGNELAIPHGTNETKDAILASALSFVRYDGGVDWDGEKV
                     TFVAHLQPSRQDVPEYAEYLERIEALVAVVNHRHGTTDWMPIDLRIYDDFDEVVARYK
                     HCDLLMVNAIFDGMNLVAKEAPVINVRDGVLMLSENTGSHEELKEFALTVNPFDIQDQ
                     ADVMCRALTMSPEERRERAEGLKRIIHSRTPADWIDEQLADIKLARTERESL"
     gene            639..>1744
                     /locus_tag="PLE93_08430"
     CDS             639..>1744
                     /locus_tag="PLE93_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH87115.1"
                     /translation="MATVRKRDFRTKILIALPVLLLALLVASGSVAGNAGARPKLRKC
                     DIGANRPMLCGHIMVPAVRGKPEAGRQKIAFVVRSRGDWSRPSLGAFVYIEGGPGYAP
                     SSPDSFKPLAAVMAPFLKRRELIAIDQRGTGRSNALFCKGLQRGTVSYQRGLKQCARQ
                     LGVRREGYTTANSAADIEAVRKALGLPKRKMILYGDSYGTYLGQSYAARYGKGMKGLI
                     LSSAYPGNDPFWRTLYPAAQRAVTRSCNRAPNCSGNALGRLNRMLRRAGTRSKLASNV
                     LNYLVGDASSYAPGGYNHLNRVITEYLRGRGRELRQLVHPYGPDQGPADYFSVGMYAA
                     VVCNDYPVPWKRSSPIPARRRQLNRAIADFRPSN"
BASE COUNT          321 a          578 c          564 g          281 t
ORIGIN      
        1 accgtctcga cctacatggg caacgagctc gcgatcccgc acggcaccaa cgagacgaag
       61 gacgcgatcc tcgcgtcggc gctgtcgttc gtgcgctacg acggcggtgt ggactgggac
      121 ggtgagaagg tcaccttcgt cgcccatctc cagccgtcaa ggcaggacgt gcccgaatac
      181 gcggagtacc tcgaacggat cgaggctctg gtcgcggtcg tcaatcaccg tcacggaacc
      241 accgactgga tgccgatcga cctgcggatc tacgacgact tcgacgaggt cgtcgcccgc
      301 tacaagcatt gcgacctgct gatggtcaac gcgattttcg acggcatgaa tctggttgcc
      361 aaggaagctc cggtaatcaa cgtgagagac ggcgtgttga tgctgtccga gaacaccggc
      421 tcgcatgagg agctgaagga gttcgccctg acggtcaatc ccttcgacat ccaggaccag
      481 gccgatgtca tgtgccgggc gctgaccatg tcaccggagg aaaggcgcga acgggccgaa
      541 ggcctgaaac ggatcatcca ttcacggact ccggccgact ggatcgacga gcagctcgcc
      601 gacatcaagc tggcccgaac cgaacgggaa agcctctgat ggcaaccgtc cggaagcgcg
      661 atttccggac gaagattctc attgccctgc cggttctcct gctcgcgctc ctcgtcgcct
      721 cgggctcggt ggccgggaac gccggggcga ggccgaagct gaggaaatgc gacatcggcg
      781 ccaaccggcc gatgctttgc ggtcacatca tggtgccggc ggtgcggggc aaacctgaag
      841 caggccggca gaagatcgcc ttcgtggtcc gcagccgggg ggactggagc cggccctcgc
      901 tgggtgcgtt cgtctacatc gaaggcgggc ccggctacgc cccgagcagc cccgattcgt
      961 tcaagccgct ggccgcggtg atggccccgt ttctgaagcg gcgcgaactg atcgcgatcg
     1021 accagcgcgg cacgggccgc tcgaatgccc tcttctgcaa gggccttcag cgcggcacgg
     1081 tcagctatca gcgcgggttg aagcaatgcg ccaggcaact gggcgtgcgg cgagaggggt
     1141 acacgaccgc caactcggcg gcggacatcg aagcggtgag aaaggcgctg ggtctgccga
     1201 agcggaagat gattctctac ggcgactcct acggcaccta ccttggccag tcctacgcag
     1261 cccggtacgg caagggcatg aagggcctga tcctttcctc cgcctatccc ggcaacgacc
     1321 cgttctggcg caccctctac ccggccgcgc agcgcgcggt gacccgttcc tgcaatcggg
     1381 cgccgaactg ctccggcaac gcactgggcc ggctcaaccg catgctgcgc cgggccggga
     1441 ccaggtcaaa actcgcttca aatgtcctga actacctggt cggtgatgcc tcgagctacg
     1501 cgccgggcgg ttacaaccac ctcaaccggg tgatcaccga atacctgcgc ggccggggcc
     1561 gggagctgcg ccagctcgtc cacccatacg gcccggacca ggggccggcc gattacttct
     1621 cggtcgggat gtacgcggcc gtggtctgta acgactaccc cgtgccgtgg aagcgttcct
     1681 ctccgatccc ggcccggcgc cggcagctga accgggcaat cgccgacttc cggccgtcga
     1741 actg
//
LOCUS       DAOQFI010000488         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44716_length_1744_cov_3.340438, whole genome shotgun sequence.
ACCESSION   DAOQFI010000488 DAOQFI010000000
VERSION     DAOQFI010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44716_length_1744_cov_3.340438"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PLE93_08435"
     CDS             complement(<1..1189)
                     /locus_tag="PLE93_08435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNH87116.1"
                     /translation="MTTRRDAPAATVAVLFFTLIFGSLTWFAASSSEAATTPVPCAPG
                     SASCFAAAEGKAAPAATDVPTSPATTPVQTPPDTGGGDQTGSDGGAVSTPGTGGVVPG
                     ETGGSGDGNGVTDPDGSTGEITPGGSEPDFVQQDPGLTGGAGPDSSVDTDPMTSDVLT
                     VPNFVIDQFEIPPFLLPIYQACGSEYGIPWYVLASINRTETSFGTNVATSSAGANGWM
                     AFMPASWAEWGVDANGDKVRDPYNPVDAICSAANYLKYFGYEQSPYDAIFAYNHADWY
                     VQIILRYAKIYSQIPPEVISALTGLTEGARFPVAAADASYEGEVSTEAAKKEGTASQD
                     IQSSSDRQTIAITASGGSPVIAVNDGTITDVNQETGTVVLEDAYGNRYSYAGLGSVST
                     VHPV"
     gene            1329..1670
                     /locus_tag="PLE93_08440"
     CDS             1329..1670
                     /locus_tag="PLE93_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87117.1"
                     /translation="MSAENDANEGIRDRVQSKGEKAAADLAQALIGSPAFGQAVSAAV
                     AARDRAAEAQRAAMGAMGVSSKDEEERLERRVRVLSDRLEETEDRLDAALDQIRELRA
                     ELKSSGSRKKT"
BASE COUNT          289 a          531 c          628 g          296 t
ORIGIN      
        1 gtaccgggtg gacggttgaa accgaaccga ggccggcgta ggagtaccgg ttgccgtagg
       61 cgtcctcgag cacgaccgtg ccggtttcct ggttgacgtc ggtgatcgtg ccgtcgttga
      121 ccgcgatcac gggcgagccg cccgaggcgg tgatcgcgat ggtctggcga tcggacgagg
      181 actggatgtc ctgggaggcg gtgccttcct tcttcgcggc ttcggtcgat acctcgcctt
      241 cgtaggaagc gtcagcggcg gcgaccggga agcgggcccc ttcggtcaga ccggtcaggg
      301 cgctgatcac ctcgggcggg atctgcgagt aaatcttcgc gtaccgcagg atgatctgga
      361 cgtaccagtc ggcgtggttg taggcgaaga tcgcgtcgta cgggctctgc tcgtagccga
      421 agtacttgag gtagttggcg gccgagcaga ttgcgtcgac cgggttgtag gggtcccgga
      481 ccttgtcccc gttggcgtcc acgccccact cggcccagga ggcgggcatg aaggccatcc
      541 agccatttgc tccggccgag gaggtggcca cgttcgtgcc gaagctggtc tcggtccggt
      601 tgatcgaggc gagcacgtac cagggaattc cgtactcgct gccgcaggcc tggtagatcg
      661 ggaggaggaa gggcggaatc tcgaactggt cgatcacgaa gttgggaacc gtgaggacat
      721 ccgaggtcat cgggtcggtg tcaacgctgc tgtcgggccc ggcgccgccg gtcaggccgg
      781 ggtcctgctg gacgaagtcg ggttcgctgc cgccgggggt gatctcgccg gtgcttccgt
      841 cggggtcggt caccccgttg ccgtcgccgc tgccgccggt ctcgccgggc accactccgc
      901 cggtgccggg tgtgctcacg gcgccgccgt cgctgcccgt ctgatcaccg ccgccggtgt
      961 ccgggggggt ctggaccggt gtggtcgcgg gggaggtcgg aacgtcggtg gcggccggtg
     1021 ccgccttgcc ctcggctgcc gcgaagcagc tggccgatcc cggagcacag ggaaccggag
     1081 tcgtggcggc ttcggaagag gacgccgcaa accaagtcag ggagccgaaa atgagcgtga
     1141 aaaagaggac cgctactgtg gcggccggcg catcgcggcg tgtagtcatt gaaccctggt
     1201 ccacctttgg cttcccaact aaaacagaca cggccgacgc cctcccgtga cgcggcagag
     1261 gaaccgtatc gcaacggctt tcgccggtac ctcgacactt cctgcgaagc gccctttaag
     1321 atcgtgtcat gtcagctgaa aacgatgcca acgagggaat cagggaccgg gtccagtcga
     1381 agggcgagaa ggccgcggcc gacctcgccc aggcgctgat cggcagtccc gcattcggcc
     1441 aggcggtctc cgctgcggtg gccgcacggg atcgcgccgc cgaagcacag cgggcggcga
     1501 tgggtgccat gggcgtctct tcgaaagacg aggaagagcg gctcgaacgg cgggtccggg
     1561 tcctttctga ccgtctcgaa gagaccgaag accggctcga cgcggcactc gaccagattc
     1621 gggagctgcg agcggagctg aaatcgtcag gaagccggaa aaagacctga cctaaacctc
     1681 aacttgaggg ttaggttcta gccttgccaa tcggctgagc accgccttgc cggcgctccg
     1741 gaac
//
LOCUS       DAOQFI010000489         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44735_length_1744_cov_2.470693, whole genome shotgun sequence.
ACCESSION   DAOQFI010000489 DAOQFI010000000
VERSION     DAOQFI010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44735_length_1744_cov_2.470693"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLE93_08445"
     CDS             complement(<1..829)
                     /locus_tag="PLE93_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87118.1"
                     /translation="MGGSSDGTLAGADRQERRWFRAFGLLLAASAALLFAFRANIGFF
                     IDDWMLIFFRHGASDWLLPHHDHPIMIPAALYDLSLSVFGMEPMPLHLVALALFLASV
                     VMLFQWIRTLVGTPAAVLGCAVVLLLGSSTEDLIWTFQIGFCGSAVTGLGALLLLRRE
                     NTGSDALACLLLVASFLLSTLAMPFLVGAAVQLCFRGGNPGLRRMLRDSWIVLVPLLL
                     YGIWLLGWNQSGGHAATLENALKTPFFVLSAFGYGSAALNRTFPLRLINDSYLWSIAG
                     "
     gene            complement(841..>1744)
                     /locus_tag="PLE93_08450"
     CDS             complement(841..>1744)
                     /locus_tag="PLE93_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87119.1"
                     /translation="TGTFPLRLINDSYLWSIAGLAVAAGFAWILQLRRRIPPEFLIAL
                     AAGLTFWILCGLNFTPGRDFFTGRYQYPSVIFILMMLAGACAGLRPDRNWLKWLTVLA
                     AASIAVNIFALFYTFNHTIKPFEQVNIVTLAAFKLSGDAIDPDFQTPIGTGDDAPLTV
                     PIYRDAVARYGPPAFTESDIDSATADYRSRLDQLLVPALRIGPEDGLSVTPVRSTCET
                     VTADAGATDTAPVAGPLLYIRPARDAVVRLTRFGPAPGAPAWLVPGGRASGYRIPADD
                     SDRPWRIGFQGRGEVLLCEATRAG"
BASE COUNT          341 a          536 c          642 g          225 t
ORIGIN      
        1 gcccggcgat cgaccagagg tagctgtcat tgatcaggcg cagcggaaag gtgcggttca
       61 gcgcggccga gccgtagccg aaggcggaga gcacgaagaa cggggtcttc agcgcgttct
      121 cgagggtggc ggcgtgtccg ccggactggt tccagccgag cagccagatg ccatagagga
      181 gaagcggcac cagcacgatc cagctgtccc tgagcatgcg gcgaagtccc gggttaccgc
      241 cgcggaagca gagctggacg gccgcgccga cgaggaacgg catggccagg gtggagagca
      301 gaaaggaggc gaccaggagc aggcaggcga gggcgtcgct ccccgtgttc tcccggcgga
      361 gcagcagcag ggcaccaagg ccggtgaccg cggacccgca gaaaccgatc tggaaggtcc
      421 agatcaggtc ctcggtcgag gagccgagaa gcagcacgac cgcgcagccg agcaccgcgg
      481 ccggggtccc gaccagggtt cggatccact ggaacagcat gaccacgctg gccaggaaga
      541 gagccagtgc gaccaggtga agcggcatcg gctccatccc gaagaccgaa agcgagaggt
      601 cgtacagcgc cgccggaatc atgatcgggt ggtcgtggtg gggcagcagc cagtcggagg
      661 ctccgtgccg gaagaagatc agcatccagt cgtcgatgaa gaagccgatg ttcgcccgga
      721 aggcgaacag cagcgccgcc gaggcagcga gcagcaggcc gaatgcccgg aaccagcgtc
      781 gctcctgcct gtccgctccg gcgagcgttc cgtcgctgct ccctcccatt ggcgagaagg
      841 ttacccggcc cgggttgctt cgcagagcag gacctcaccc cggccctgga aaccgatccg
      901 ccagggccgg tccgaatcat cggccgggat ccggtagccg ctcgcccgcc ctccgggcac
      961 cagccaggcg ggcgctcccg gggcggggcc gaaccgggtc agccgcacca ccgcatcgcg
     1021 ggcgggcctg atgtaaagca ggggaccagc aaccggcgcc gtgtcggtcg cgccggcgtc
     1081 ggcggtgacc gtctcgcagg ttgagcggac cggagttacc gaaaggccgt cctcgggccc
     1141 gatccggagg gccgggacga gcagctggtc gagtcgcgac cggtaatccg ccgtggccga
     1201 atcgatgtcg ctctcggtga atgcgggagg tccgtaccgg gcgaccgcgt cccggtagat
     1261 ggggaccgtc agaggtgcgt catcaccggt cccgatcggg gtctggaagt ccggatcaat
     1321 cgcatccccc gacagcttga aggcggccag ggtgacgatg ttgacctgct cgaaaggctt
     1381 gatcgtgtgg ttgaaggtgt agaagagggc gaagatgttg acggcgatcg aggctgccgc
     1441 gagtacggtc agccacttca gccagttgcg gtcgggccgg agcccggcgc atgccccggc
     1501 cagcatcatc aggatgaaga tcacgctcgg gtactggtag cggccggtaa agaaatcccg
     1561 tccgggagtg aagttgagcc cgcaaaggat ccagaaggtg aggcccgccg ccagggcgat
     1621 caggaactcg ggcgggatcc ttcggcgaag ctggaggatc caggcgaaac cggccgcaac
     1681 ggcgagcccg gcgatcgacc agaggtagct gtcattgatc aggcgcagcg gaaaggtgcc
     1741 ggtc
//
LOCUS       DAOQFI010000490         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44767_length_1743_cov_3.538507, whole genome shotgun sequence.
ACCESSION   DAOQFI010000490 DAOQFI010000000
VERSION     DAOQFI010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44767_length_1743_cov_3.538507"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..645)
                     /locus_tag="PLE93_08455"
     CDS             complement(244..645)
                     /locus_tag="PLE93_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNH87120.1"
                     /translation="MDVQRKRQVRLFVALGAALLLATGLIYTSFNASSEARQPSELVD
                     AVPGKTYEMTGKVVKDSVVKTGNGIRFKVKDRDGTGEALPVTYRGVVPDPFREGREIV
                     LTGTVENGVFVGEPDTLVTKCPSKFSEKQDQ"
     gene            706..1410
                     /locus_tag="PLE93_08460"
     CDS             706..1410
                     /locus_tag="PLE93_08460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH87121.1"
                     /translation="MSEEKVLLITGASSGIGRATTRLMAGDRRLVLAGRRVEPLEELA
                     AELGGPERALVVGCDVGEWDQVETMTAAAIEAFGRIDEVFANAGFGASRGFLEESPEH
                     WKSMVQTNVLGTALTIRSTLPHLLERGSGHFIVTSSIAGRRVLPGSLYSATKFATTAI
                     GEALRQELRSRDDGKNIKVTLIEPGMVDTDFFENRPGESALKDEDIARTIQFALDQPE
                     GVDLNEILIRPISQAT"
     gene            complement(1523..>1743)
                     /locus_tag="PLE93_08465"
     CDS             complement(1523..>1743)
                     /locus_tag="PLE93_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87122.1"
                     /translation="LKSYRDLIRWLPRLRRERAQIQSARGIPASQFADLLTPDLDSPL
                     IPEFARKGLVRAMLRTYWRVVRTLLARR"
BASE COUNT          317 a          571 c          570 g          285 t
ORIGIN      
        1 ccctgcgtgg cgatgctgcc catcttcggg ccggagtggg tggcgaccac gttgaagctg
       61 aagtcgtcgg tcaggaaggc gtgttcgatc acgaccacgc agagcaccat cagggcgcag
      121 aaggcgtaga ccgcgtagcg ggaggagtca acccagcgtc ggtcgcccct ggttccggcc
      181 agcgctgcga ccgccccgaa tgtcgcggtc agcagggcaa cccagagcgc cgcgttgcct
      241 aagttactga tcctgcttct ccgagaactt cgaggggcac ttggtcacga gcgtgtccgg
      301 ttcccccacg aagaccccgt tttcgacagt gcccgtgagc acgatttcgc gcccttcgcg
      361 gaaggggtcg ggcacgaccc ctctgtaggt gacgggcagc gcctcgccgg ttccgtcacg
      421 gtccttcacc ttgaacctga tgccgttccc ggtcttcact acggagtcct tgaccacctt
      481 gccggtcatc tcataggtct ttccgggcac ggcatcgacc agctcactgg gctgacgggc
      541 ctcggaagaa gcattgaagg aggtgtaaat cagtccggtg gccagaagga gggcggcgcc
      601 cagagccacg aacaggcgca cctgccgctt gcgctgtaca tccataacgg aaggtattat
      661 cgcaagcctt ggcggccggc ggcttccccc tgacagaatc cgcatatgag cgaagagaag
      721 gtacttctga tcaccggagc aagcagcggc atcggcaggg ccacgacccg cctgatggcc
      781 ggggacaggc ggctggttct ggccggccgc cgggtcgagc cgctggagga actggcggcg
      841 gaactgggcg ggcccgagcg ggccctggtc gtcggctgcg acgtcgggga atgggaccag
      901 gtcgagacca tgaccgccgc ggcgatcgag gcattcggcc ggatcgacga ggtcttcgcc
      961 aacgccggct tcggggccag tcgcggcttc ctcgaggagt caccggaaca ctggaagtca
     1021 atggtccaga ccaacgtgct cgggaccgcc ctgaccatcc gttccaccct gccccacctg
     1081 ctcgaacggg gcagcggcca tttcatcgtg accagctcga tcgccggccg ccgggtcctg
     1141 cccggctcgc tctactcggc gaccaagttc gcgaccaccg cgatcgggga agcgctgcgg
     1201 caggaactgc ggagccggga tgacggcaag aacatcaagg tgaccctgat cgagccgggc
     1261 atggttgaca cggacttctt cgagaaccgc cccggcgaat cggccctcaa ggacgaagac
     1321 atcgcccgca cgatccagtt cgccctcgac cagcctgaag gcgtcgacct gaacgagatc
     1381 ctgatccgcc cgatctccca ggcgacttaa ctgcagtcgc gccttcggcg ctcccacagc
     1441 tttcacaccg gttggggatc cggacttcga gcaggcaccc gcccgatcac gatcatcaac
     1501 aagtgatgat cgggctccat ggctacctcc tggcgaggag ggtccggaca actcgccagt
     1561 aggtgcggag cattgccctc accaaccctt ttcgggcgaa ttcgggaatc aggggtgaat
     1621 cgaggtcggg tgtcaggagg tcggcgaact ggctggccgg gatcccccgg gccgactgga
     1681 tctgcgcccg ctcccgcctc aggcggggaa gccagcggat caggtcgcgg taggacttca
     1741 gct
//
LOCUS       DAOQFI010000491         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44823_length_1742_cov_3.415531, whole genome shotgun sequence.
ACCESSION   DAOQFI010000491 DAOQFI010000000
VERSION     DAOQFI010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44823_length_1742_cov_3.415531"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..831)
                     /gene="rsmG"
                     /locus_tag="PLE93_08470"
     CDS             complement(85..831)
                     /gene="rsmG"
                     /locus_tag="PLE93_08470"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNH87123.1"
                     /translation="MNSVTPPPPPAGPSAGEGTTGGEAEPWLDRAALARLDPMLDLLA
                     ESHVSLSSVTDPAEARRVHVADSLSGLACPEVRAAKRCADLGAGAGFPGIPLAVFLPE
                     CEFVLIDSVGRKVDFMNEAISALGLENARAIKARSEDFAKGEGREAFDLVTARAVAPL
                     AALAELASPLLREGGHLLAWKGEPEPDSEQVIAANRDRLAMEIEGVREVMPYEGSRDR
                     RLYLLVKAGPTPDGLPRRPGMARKRPLGGG"
     gene            complement(842..1321)
                     /locus_tag="PLE93_08475"
     CDS             complement(842..1321)
                     /locus_tag="PLE93_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNH87124.1"
                     /translation="MEASEWPAEPAERVRLLVERVLDELDLDGEVEMDETDDVITATV
                     EGDEDYGLLIGRRGQTIDALQLLAFQAAFRGIRERKRVVVDAAGYRARREEVISERAD
                     RAAEKAIASGSEVELDPMSARERRIVHEHLKSRPEVETFSSGDDPHRRVVIAPLITE"
     gene            complement(1353..>1742)
                     /locus_tag="PLE93_08480"
     CDS             complement(1353..>1742)
                     /locus_tag="PLE93_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNH87125.1"
                     /translation="PAIHALYPFGESFLFIPDLTDKATGGVLIALLVLYVGTQLISGV
                     ISMTTGDRNQRMLMLALPFIFVPFIISFPAGLILYWITTNVWTIGQTYVINRVIPPPV
                     MSTPEGVVVAAAAKPPPPPPKKKKRKR"
BASE COUNT          290 a          565 c          601 g          286 t
ORIGIN      
        1 ggggtgaatg gcaacatcat cggctccgac ccgaacttgg gctcactcca gcttatttgt
       61 ttgccggaag cgaccggtca ctggtcaacc gccgcccaac ggtcgctttc tggccatgcc
      121 cgggcgcctg gggaggccgt ccggggttgg ccctgccttg accagcaggt agaggcgccg
      181 gtcgcgtgat ccctcgtagg gcattacttc ccggaccccc tcgatttcca tcgcgaggcg
      241 atcccggttg gcggcgatga cctgttccga gtcgggttcc ggctcaccct tccaggcgag
      301 aaggtgccct ccctcgcgaa gcaggggaga ggcgagttcg gccagggcgg caagcggagc
      361 aaccgcccgc gccgtgacga gatcaaacgc ctcgcgcccc tcgcccttcg cgaaatcttc
      421 cgaccgggcc ttgatcgccc gggcattttc cagtcccaga gccgaaatcg cctcattcat
      481 gaaatcgacc ttgcggccca cggaatcgat caggacgaac tcgcattcgg gcaggaagac
      541 ggccagcggg atgccgggga agccggctcc ggcaccgagg tccgcgcagc gcttcgctgc
      601 cctgacttcg gggcaggcca ggcccgagag actgtcggcc acgtggaccc ggcgggcttc
      661 ggccggatcg gtcaccgaac tgagcgagac atggctctcc gcgagcaggt cgagcatcgg
      721 gtccaggcgg gccagggccg cccggtccag ccagggctct gcttcgccgc cggtcgtgcc
      781 ttcgccggcg gaggggcctg cgggcggggg tggaggggtg accgaattca cccggccagg
      841 gtcactcggt gatcaggggt gcgatcacga cccgccgatg cggatcatca ccggaactga
      901 aggtctcgac ctcgggccgg ctcttcaggt gctcgtgaac gatccggcgc tcgcgtgcgc
      961 tcatcggatc gagttccacc tcggagccgg aggcgatcgc cttctcggcg gcgcgatcgg
     1021 cccgctcgga gatcacttcc tcacgccggg cccggtagcc ggctgcgtcc accacaaccc
     1081 gcttgcgttc gcggatcccg cggaaagcgg cctggaaagc caaaagctgg agcgcgtcga
     1141 tggtctggcc gcgacggccg atcagcaggc cgtagtcctc gtcaccctcc accgtggcgg
     1201 tgatgacgtc atcggtctcg tccatttcaa cctcgccgtc gaggtcgagc tcgtcgagaa
     1261 cccgctcgac caggagtcgc acccgttctg ccggttcagc cggccattcg ctcgcttcca
     1321 tcgctcgcag tctacccgga gaaagcaggc gctcagcgct tgcgcttctt cttcttgggg
     1381 ggcggaggcg ggggcttcgc cgccgcggcg acgacgacac cttcaggggt ggacatcacc
     1441 ggcgggggga tgacccggtt gatcacgtag gtctggccga tcgtccagac gttggtcgtg
     1501 atccagtaga ggatcaggcc ggccgggaag gagatgatga agggaacgaa gatgaagggg
     1561 agagccagca tcagcatgcg ctggttgcga tcgccggtgg tcatcgagat gacgcccgag
     1621 atcagctggg ttccgacgta gaggacgagc agcgcgatca gcacgccgcc ggtcgccttg
     1681 tcggtcaggt cgggaatgaa gaggaacgac tcgccgaagg ggtaaagcgc atgaatggcg
     1741 gg
//
LOCUS       DAOQFI010000492         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_44975_length_1739_cov_3.491686, whole genome shotgun sequence.
ACCESSION   DAOQFI010000492 DAOQFI010000000
VERSION     DAOQFI010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44975_length_1739_cov_3.491686"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="PLE93_08485"
     CDS             <1..1386
                     /locus_tag="PLE93_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNH87126.1"
                     /translation="RPGAFGCAAVASNGWRAWPTGQADNSAPSTTISSSPRPSNAPAT
                     VDFEFSSSETGSQFECRLDDGGNTAGYEDCASPMRYGPLGDDLYTFSVRATDAAGNTG
                     GPATVKFRTGGPAPVLAISPAVFAFGPINVGGELGEPGEFTLSNPGSDPVNLDDGTLT
                     GPDAGSFKLLPNGSTCYFLEPAGVLPPGGSCTLVAIFDPESEGPKTASLEVTSDSVTS
                     PDQLSLSGTGLPELLPGASVTPGTLAFGNRQAGTDSPARTITLTSTGSGDLEIGNLIR
                     LGADASQFAVTGNTCSGRSMASGDSCQVQVVFSPVGTGARSATLTIPTDASGADTSVD
                     LTGTATAPPVVHRNSRVQIGGSLPRLRGSRQLRIPVRCFTTNMEFCRGRLRVKARGRA
                     LGLRHNRLVQVATDTYELRAGQRTVRVRIGNRARRRVLRRGSLPVRVIGTSRQGNGSF
                     RSVNAGRRLRR"
     gene            1445..>1739
                     /locus_tag="PLE93_08490"
     CDS             1445..>1739
                     /locus_tag="PLE93_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87127.1"
                     /translation="MRRTGGILAAILIVGAIAAVPASGANAKPRAVVPSTSTIFGYIK
                     DLTSFGYRRTGSKASLKAATYVRDSFRRFGLSEVHFQTARSYAWKVSDSSLSVD"
BASE COUNT          276 a          613 c          570 g          280 t
ORIGIN      
        1 aggccgggcg ccttcgggtg cgccgccgtc gcgagcaatg gctggcgggc ctggccgacc
       61 ggacaggccg acaacagcgc tccctcaacc acgatctctt cgtcgccgcg gccatcgaat
      121 gccccggcca cagtcgactt cgagttctcg tcaagcgaga ccggttcgca gttcgagtgc
      181 cgcctggatg acggcggcaa taccgccggc tacgaagact gcgcctcacc gatgcgttac
      241 ggcccgctcg gggacgacct ctacaccttc tctgtccggg cgacggacgc cgccggaaac
      301 accggcggtc cggccacggt gaagttccgc accggtggcc cggctccggt gctcgccatc
      361 agcccggccg tgttcgcctt cggcccgatc aacgtcggcg gtgagctggg agagccgggt
      421 gaattcacgc tcagcaaccc gggaagcgat cccgtcaacc tagatgacgg gaccctcacc
      481 ggacccgacg ccggttcctt caagctcctg cccaacggca gcacctgcta cttcctcgag
      541 ccggccgggg ttctcccgcc gggaggttcc tgcacgctcg tggcgatctt cgaccccgaa
      601 agtgaagggc cgaagaccgc cagcctcgag gtcacctccg attcggtcac ctcccccgac
      661 cagttgtccc tgtcgggaac cggccttccc gagctgcttc ccggtgcctc ggtcacgccc
      721 gggacgctgg ccttcgggaa ccggcaggcg ggcaccgact ctccggcccg gacgatcacc
      781 ctgaccagca ccggcagcgg cgatctcgag atcggcaacc tgatcaggct cggcgccgac
      841 gccagtcagt tcgcggtcac cggcaacacc tgctcgggcc gctccatggc gagcggtgat
      901 tcctgccagg tccaggtcgt cttctcgccg gtcgggaccg gggcgaggag cgccaccctg
      961 accattccga ccgacgcctc cggggccgac acctcagttg acctgaccgg aaccgcaacg
     1021 gcaccgcctg tggttcaccg caacagccgc gtccagatcg gtggcagcct gccgcgcctg
     1081 cgtgggtcca gacagttgag gatcccggtc cggtgcttca cgaccaacat ggaattctgc
     1141 cgcggaaggt tgcgggtgaa ggccaggggc cgtgcgctgg ggctgcgtca taaccggctg
     1201 gtccaggtcg cgaccgatac atacgagctg cgggcaggtc agcggacggt ccgggtgagg
     1261 atcggcaacc gggcccggcg ccgggtgctc cgccggggca gcctgccggt gcgggtgatc
     1321 ggtacttcgc gccagggcaa cggcagtttc cggtccgtga acgccggccg tcgcctgagg
     1381 cgctgaaact cgaacctccc ggttgaccgg cggggtctat ccttgtcggc atggaggagg
     1441 gagcatgagg cgaaccggag ggattcttgc cgcgatcctg atcgtcgggg caatcgctgc
     1501 cgtgccggca tcgggagcga acgcaaagcc cagggcggtg gtgccttcga ccagcacgat
     1561 cttcggctac atcaaggacc tgacttcctt cgggtaccgg cgcaccggtt cgaaagcgag
     1621 cctcaaggcc gcgacctatg tgcgggacag tttccgacga ttcggtcttt ctgaagtcca
     1681 cttccagacc gcccgctcct acgcctggaa ggtcagcgac tcctcccttt cggtggacg
//
LOCUS       DAOQFI010000493         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_45151_length_1736_cov_2.677573, whole genome shotgun sequence.
ACCESSION   DAOQFI010000493 DAOQFI010000000
VERSION     DAOQFI010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45151_length_1736_cov_2.677573"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..915)
                     /locus_tag="PLE93_08495"
     CDS             complement(76..915)
                     /locus_tag="PLE93_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNH87128.1"
                     /translation="MPEPLRRFGAACWEVLLSFDRSAGTRQAAQLSFFVLLSFPALIL
                     LAVWILSNIFDSPDVRQDLIREIINNLPIEEIQGKREIEDMLDELTKGAGGLGIFSIV
                     ILLYSVSSAIGGLRHAVETANEKGDSGPPFPQNKLLDIGITLVTLPAALIFVALALSR
                     DLARVVDDNVFLSWSADHLGGPLGIFAAALIFYTWIFWVLNPGPTSWTSAAAGSLVTS
                     ALIWLVYSGLRIWFGISGGGSAIYGALAGFLGLLLFLNLASIAVVMGAHVAAIWRVHR
                     EAG"
     gene            complement(959..1387)
                     /locus_tag="PLE93_08500"
     CDS             complement(959..1387)
                     /locus_tag="PLE93_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87129.1"
                     /translation="MPETSGSQPPGGSAAWASWVAFAGIMLMLIGVFSVIAGLAAVTD
                     DGYIANASLHEVFLVSFKALGVFWILLGILKVLAGWALIQGREWARIVTVILVFLSAI
                     SHLLDLTSQPYLSILMIALDLVILYAVTVRWEAAKIGMGD"
     gene            complement(1453..>1736)
                     /locus_tag="PLE93_08505"
     CDS             complement(1453..>1736)
                     /locus_tag="PLE93_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNH87130.1"
                     /translation="EITGNAWEWTKSWYADSHPEPVSRCCAPENPLGPGVEASRDPAS
                     GIPRKVLKGGSFLCAENYCSRYRPSSRIPESIESSTVHITFRCVVPDAG"
BASE COUNT          353 a          543 c          572 g          268 t
ORIGIN      
        1 gatcaggatg aagaagagcg ccagtcgcgg cgagatcagt gcgaagagaa ctacgaagca
       61 acccatgcct gcaatctacc cggcctcacg gtggacccgc cagatcgccg ccacgtgagc
      121 ccccatcacg accgcgatcg aggccaggtt gaggaagagc aggaggccga ggaacccggc
      181 cagcgccccg tagatcgcgc tgccgccgcc cgagatcccg aaccagatcc gcaggcccga
      241 gtacacgagc cagatcaggg ccgaagtgac cagtgaaccc gccgccgcgc tcgtccagct
      301 cgtcgggccc gggttcagca cccagaagat ccaggtgtag aagatcaggg cagcggcgaa
      361 tatgcccaac ggtccgccga ggtggtcggc gctccacgaa aggaagacgt tgtcatcgac
      421 gaccctggcg aggtcgcggg aaagggccag cgcgacgaag atcagcgccg ccggaagggt
      481 gaccagggtg atgccgatgt caagcagctt gttctggggg aagggcgggc ccgaatcgcc
      541 cttctcgttg gcggtctcga ccgcgtgacg aaggccgcca atcgccgagc tgaccgagta
      601 gagcaggatc actatggaga agattccgag cccgccggcc cccttggtca gctcatcgag
      661 catgtcctcg atttctcttt tgccctggat ctcctcgatc gggaggttgt tgatgatctc
      721 ccggatcagg tcctggcgca cgtccggcga gtcgaagatg ttgctgagga tccagaccgc
      781 gagcaggatc agggccggga agctcagcag cacgaagaac gacagctggg ccgcctgccg
      841 ggtgcctgcc gaacggtcga atgacagcag cacttcccag caggcggccc cgaaccgccg
      901 cagcggctcg ggcaccttcg aagcccaggc cttcagtttt gaagcccggg tgggcgaatc
      961 agtcccccat gccgatcttc gccgcttccc agcggacggt cacggcgtaa aggatgacca
     1021 ggtcaagagc gatcatcagg atgctcaggt agggctgcga ggtcaggtcg agcaggtggc
     1081 tgatcgccga gaggaagacc aggatcacgg tcacgatccg ggcccactcg cgaccctgga
     1141 tcaatgccca gccggcaagt accttgagga ttccaagcag gatccagaac acccctaggg
     1201 ccttgaagct gaccaggaaa acctcgtgga gggaagcgtt ggcgatgtat ccgtcatcgg
     1261 tcacggcagc caggccggcg atgacgctga acacgccgat cagcatcagc atgatgccgg
     1321 cgaatgcgac ccaggaagcc caggcggcgg atcctcccgg gggctgtgaa ccactggttt
     1381 caggcattga actcctccat cgatttcatg tttggcgggc cgccggggga cgaacgggcg
     1441 gctccatgaa ccctatccgg cgtcggggac aacgcagcgg aaggtgatgt gcacggttga
     1501 gctctcgatg ctttccggga ttcgcgagga cggccggtag cgcgagcagt agttctcggc
     1561 gcagaggaac gacccgcctt tcagcacctt gcggggaatg ccactggccg gatcacggct
     1621 cgcttcgact cccgggccga gcgggttctc gggggcgcaa cagcggctca ccggttccgg
     1681 atgcgaatcc gcgtaccagc tcttcgtcca ctcccaggcg tttccggtga tctcgt
//
LOCUS       DAOQFI010000494         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_45154_length_1736_cov_2.534206, whole genome shotgun sequence.
ACCESSION   DAOQFI010000494 DAOQFI010000000
VERSION     DAOQFI010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45154_length_1736_cov_2.534206"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLE93_08510"
     CDS             complement(<1..212)
                     /locus_tag="PLE93_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87131.1"
                     /translation="MKKFILGMTLSLFMLGAAQADAVPKYNASCMFNGCGGVSQEGQR
                     AIFTFDDMYPGPDEDFDIYERVGGQTR"
     gene            complement(299..1159)
                     /gene="gluQRS"
                     /locus_tag="PLE93_08515"
     CDS             complement(299..1159)
                     /gene="gluQRS"
                     /locus_tag="PLE93_08515"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNH87132.1"
                     /translation="MTARLDLFHPDREPEAAPGRYAPSPTGRLHLGNLRTALIAWRSA
                     RDRGARFLVRIEDIDTGRSRDEWIEVQLEELRQIGVDWDGEPVRQSERHDLYREALSK
                     LEADGLLYECFCTRAEIREAASAPHGELPEGAYPGTCRELTEAERAGRRAAGRPPALR
                     VRAAGARVGFDDHLCGRVEKTLDDFVVRRNDGDFAYNLAVTVDDADQGIGEVLRGADL
                     LDTTPRQLWLYDRLGLAAPARFSHVPLMLGEDGERLAKRHGAITLEERIEAGESPASV
                     RRELFDSADR"
     gene            complement(1156..>1736)
                     /locus_tag="PLE93_08520"
     CDS             complement(1156..>1736)
                     /locus_tag="PLE93_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNH87133.1"
                     /translation="EPEEAIEGLLEISARAHGVGTARCLRDYFRLPARESKEAIERLA
                     ADGRLIEAEVEGFSAPAFLHPEARRPRKVEARALLAPFDPLIFERRRLEELFGFHYRI
                     EIYTPKEKRRHGYYVLPFLMAEQIAGRVDLKSDREGSALLVQSAWVEPDAPAGTAAEL
                     ADELRLMAGWLGLEEVAVRPRGDLAADLAAAL"
BASE COUNT          275 a          587 c          587 g          287 t
ORIGIN      
        1 cgggtctgac cgccaacccg ctcgtagatg tcgaagtcct cgtcagggcc ggggtacatg
       61 tcatcgaagg tgaagatcgc acgctggcct tcctgggaga cgcccccgca accgttgaac
      121 atgcagctcg cgttgtactt gggcacggca tcagcctgag cggcgccgag catgaaaagg
      181 ctgagggtca tccccaggat gaacttcttc aagattttcc cctgttcgga gcgctccggc
      241 tccgtcgatt gcaatggaca aaattgaaca cggcatgttc aagaatgttg cgttgaggct
      301 accgatcagc cgaatcgaac aactccctgc ggactgacgc cgggctctct cctgcttcga
      361 tccgctcctc gagggtgatc gcgccgtggc gcttggcgag gcgctctccg tcttcgccga
      421 gcatcagggg gacatggctg aaacgggccg gggcggcaag gccgaggcgg tcgtagagcc
      481 agagctggcg gggggtggtg tcgagcaggt cggcgccgcg aagcacctcg ccgatcccct
      541 gatcggcatc gtcgacggtg accgcgaggt tgtaagcgaa gtcaccgtca ttgcggcgca
      601 ccacgaagtc atcgagggtc ttttcgaccc ggccgcagag gtgatcatcg aagccgaccc
      661 tggctccggc cgcccggacc cggagtgccg ggggccggcc ggcagcccgg cgcccggccc
      721 tctcggcctc ggtgagttcc ctacaggtgc cggggtaggc accttctggc aactcgccgt
      781 gaggggccga cgctgcttcg cggatctcgg cccgggtgca gaagcactcg tagagcaggc
      841 cgtcggcttc aagtttggaa agtgcctcgc gataaaggtc atggcgctcg ctctgacgga
      901 ccggctcgcc gtcccagtcg actccgatct ggcggagctc ctcgagctgc acttcgatcc
      961 actcgtcccg ggagcggccg gtgtcgatgt cctcgatccg gaccaggaac cgcgccccgc
     1021 gatctcgggc cgagcgccag gcgatcaggg ccgtcctcag gttgccgaga tggagccgcc
     1081 cggtcgggct cggggcgtag cggcccgggg cagcttccgg ctcccggtcg ggatgaaaca
     1141 ggtcgagcct ggcggtcaca gggccgccgc cagatccgcg gcgagatctc ccctgggccg
     1201 gaccgccacc tcttcgaggc cgagccaccc ggccatcagc ctgagttcgt cggccagttc
     1261 cgccgcggtc ccggccgggg catccggctc gacccaggcc gactgcacca gcagcgccga
     1321 tccctcccgg tcggatttga ggtccacccg gccggcgatt tgttctgcca tcagaaacgg
     1381 cagcacgtag tagccgtggc gacgcttctc cttcggggtg tagatctcga tccggtagtg
     1441 gaagccgaaa agctcctcga gtcgacgcct ctcgaagatc agcggatcga atggagcgag
     1501 cagcgcccgc gcctcgacct tgcggggtcg acgggcctcg gggtgcagga aggccggagc
     1561 cgagaaccct tcgacctcgg cctcgatcag gcgcccgtcg gcggcgagcc gttcgatcgc
     1621 ctccttcgac tcccgggccg gcaggcggaa gtagtccctg aggcagcgcg ccgtgccgac
     1681 cccgtgggcc cgggcggaga tctccagcaa tccctcgatc gcttcctccg gctcgg
//
LOCUS       DAOQFI010000495         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_45442_length_1730_cov_4.520597, whole genome shotgun sequence.
ACCESSION   DAOQFI010000495 DAOQFI010000000
VERSION     DAOQFI010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45442_length_1730_cov_4.520597"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLE93_08525"
     CDS             complement(<1..333)
                     /locus_tag="PLE93_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNH87134.1"
                     /translation="MSFPSTRLRRLRRTPALRGLVRETGLSAADLIQPLFVTAGTDLR
                     EPVESMPGVNRFSIAALVEEAGEIQAAGIPAVLVFGVPSEKDEAGSGAYDDEGVVQMA
                     VRALKESHP"
     gene            complement(449..>1730)
                     /locus_tag="PLE93_08530"
     CDS             complement(449..>1730)
                     /locus_tag="PLE93_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH87135.1"
                     /translation="LILYPIVIALLVGFAMTRDQDQTKVALYNAMPAGEPLGFGGEEV
                     DGNEIRDQLCGRVDCVEADSTADAKKMVESGEATAAVIVPEDLGDKIRSLASLNPESP
                     EIQIIVNGSDAVESRVTDDRIEAMLGEANLLLAKRLSEGAKEYLDLILQGGEFDLLGQ
                     QIEVLGLKRSEQILKQIEPKLPPGTDREQLGRAVRFAHLATDNLDLAGPLLTAITEPI
                     KAEKVEVGDDAPSLDTFAIGVTVAFALMFVTVLLVAGSLALEKEENAFTRLTAGLVSR
                     GQILVSKVGLGTVVGFVVALILLTGLGLFVGIEWGRAPYWLLALIFGAAAFSAGGAAL
                     GAAAREVRAATLAAVMICLPVALLSLVPDDAVGPVIETAINLVSAIFPFRPTLDAITG
                     GLEISGPTLWLPLLHLVILTAAYGALARLALRRF"
BASE COUNT          286 a          587 c          590 g          267 t
ORIGIN      
        1 cgggtggctc tccttcaggg cccgcaccgc catctggacc acgccctcgt cgtcgtaggc
       61 cccgctgccc gcctcgtcct tctccgaggg aaccccgaag accagcaccg ccggaatccc
      121 cgccgcctga atctcccccg cctcctcaac cagggcggcg atcgagaacc ggttgacccc
      181 cggcatcgac tcgaccggtt cgcgcaggtc ggtgccggcc gtgacgaaga gcggctggat
      241 caggtcggcc gccgacaggc cggtttcacg aaccagcccg cggagggccg gggtgcggcg
      301 cagacggcgc aggcgggtac tcggaaagct cataagtcca gtctagaagt acttcaggct
      361 gagcccctcc caaccatcag ttgtctcgaa tgggaggggc gggttcctcg tggagggcgg
      421 gtcggacccc gcccgactgg cagttaagtc aaaaacggcg tagggcgagg cgggccagcg
      481 ctccgtaggc ggcggtgagg atgaccaggt ggaggagggg gagccagagg gttggaccgg
      541 agatctcgag gccgccggtg atcgcgtcga gggttgggcg gaaggggaag atcgcgctga
      601 ccaggttgat cgcggtttcg atcaccgggc cgaccgcgtc gtcgggaacc agggagagca
      661 gagcaaccgg caggcagatc atcaccgcag ccagggtcgc cgcccgcacc tcccgggccg
      721 cggcacccag cgccgcgccg ccagccgaga aagccgccgc gccgaagatc agggcgagca
      781 gccagtaggg cgctctcccc cactcgatcc cgacgaacag ccccagcccg gtcagcagaa
      841 tcagggccac cacgaagccg accacggttc ccagaccgac cttgctcacg aggatctggc
      901 cgcggctgac cagaccggcg gtgagccggg tgaaggcgtt ctcctccttt tcgagcgcca
      961 gcgatccggc gaccagcagg acggtcacga acatcagcgc gaaggcgacg gtcaccccga
     1021 ttgcgaaggt gtccagcgag ggcgcgtcat caccgacctc gaccttctcg gctttgatcg
     1081 gttcggtgat cgcggtcaaa agcggaccgg ccaggtccag gttgtcggtc gccaggtggg
     1141 cgaagcggac cgcccggccg agctgctccc tgtcggttcc cggcggcagc ttcggctcga
     1201 tctgcttgag gatctgctcg ctcctcttca gcccgagaac ctcgatctgc tggccgagca
     1261 ggtcgaactc gccgccctgg aggatcaggt cgagatactc cttcgcgcct tcggagaggc
     1321 gcttggccag cagcaggttc gcctcgccga gcatcgcttc gatccggtcg tcggtgaccc
     1381 ggctttcgac cgcgtcggag ccgttgacga tgatctggat ctccggtgac tcggggttga
     1441 gcgaggcgag ggagcggatc ttgtcgccga ggtcttccgg cacgatcacc gcggcggtgg
     1501 cctcgccgct ttcgaccatc ttcttcgcgt ccgcggtgct gtcggcttca acgcaatcga
     1561 ccctcccgca gagctggtcg cggatctcgt tgccgtcgac ctcctccccg ccgaagccga
     1621 gcggctcacc ggccggcatc gcgttgtaga gggcgacctt ggtctggtcc tggtcgcggg
     1681 tcatcgcgaa gccgaccagc agggcgatca cgatcggata gaggatcagc
//
LOCUS       DAOQFI010000496         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_45495_length_1729_cov_3.514337, whole genome shotgun sequence.
ACCESSION   DAOQFI010000496 DAOQFI010000000
VERSION     DAOQFI010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45495_length_1729_cov_3.514337"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLE93_08535"
     CDS             <1..518
                     /locus_tag="PLE93_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNH87136.1"
                     /translation="VRSAQVSRPGETNGDSDQLEVTVRYLTPHSKWSIHSEFQDNLHM
                     LTLFRGGPVMWISEKDAERVEVKDNDWLEVYNSHGAVSCRAAVSVRVPEGIALFYHSQ
                     DRHVNVPISEVTGQRGGTDNSLTRISMKPTHMIGGYAQLSYGFNYYGPCGTQRDQLIM
                     IRKRQEEVRYQ"
     gene            515..>1729
                     /gene="narH"
                     /locus_tag="PLE93_08540"
     CDS             515..>1729
                     /gene="narH"
                     /locus_tag="PLE93_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNH87137.1"
                     /translation="MKVRAQMGMVMNLDKCIGCHTCSVTCKNVWTSRRGMEYVWFNNV
                     ETKPGRGFPVDYEDQSKWKGGWKLDRRGRLKLRAGGPLHKLLNIFSNPDLPELDDYYD
                     PWTYDYDKLISSPSSEHQPVARPKSQVTGDDLDIQWGPNWEDDLAGSAEHAAFDPNFR
                     NIQEEVKQGFEDAFMMYLPRICEHCVNPSCVASCPSGAMYKREEDGIVLVDQDACRSW
                     RFCVSGCPYKKVYFNWNTGKAEKCNFCYPRIEAGMPTVCSETCVGRLRHLGVVLIDTD
                     RVEAAASVENEHDLLNAQLDLMLDPDDPEVREQALRDGIPADWIDAASYSPVYAMAKK
                     WKIALPLHPEYRTLPMVWYVPPLSPMESVGKALVRDGEDADAENVFAAIDEMRIPVEY
                     LASMLSAGDVEPV"
BASE COUNT          332 a          567 c          543 g          287 t
ORIGIN      
        1 cggtccggtc ggcccaggta tcgaggccgg gtgagaccaa cggcgactcc gaccagctcg
       61 aggtcacggt ccggtacctg accccgcact cgaaatggtc gatccactcc gagttccagg
      121 acaacctcca catgctgacc ctgttccgcg gtggcccggt gatgtggatc tcggagaagg
      181 acgccgagcg ggtcgaggtc aaggacaacg actggctcga ggtctacaac tcccacggcg
      241 ccgtctcctg ccgggccgcg gtctcggtgc gggtgcccga ggggatcgcg ctcttctacc
      301 actcgcagga ccgtcacgtg aacgtgccga tttcggaggt gacaggtcag cgcggaggca
      361 ccgacaactc actgaccagg atttcgatga aaccgaccca catgatcggg ggctacgccc
      421 agctctccta cgggttcaac tactacggcc cttgcgggac acagcgtgac cagctgatca
      481 tgatccgcaa acgccaggag gaggttcgct accagtgaag gtccgggccc agatgggcat
      541 ggtcatgaac ctcgacaagt gcatcggctg ccacacctgt tcggtgacct gcaagaacgt
      601 gtggaccagc cggcgcggca tggagtacgt ctggttcaac aacgttgaaa ccaagccggg
      661 tcgtggcttt cccgtcgact acgaagacca gtcgaaatgg aagggcggct ggaaactgga
      721 ccgccgtggc cggctcaagc tgagggcagg ggggccgctt cacaagctgc tcaacatctt
      781 ctccaacccg gacctgccgg aacttgacga ttactacgac ccctggacct acgactacga
      841 caagctgatc tcctcgcctt cgagcgagca ccagccggtt gccaggccga agtcgcaggt
      901 aaccggtgac gacctcgaca tccagtgggg tccgaactgg gaagacgacc tcgccggctc
      961 ggccgaacac gccgcctttg acccgaactt ccgcaacatc caggaagagg tcaagcaggg
     1021 cttcgaggac gctttcatga tgtacctgcc gcggatctgc gagcactgcg tcaacccgtc
     1081 ctgcgtcgcc tcctgcccct ccggcgcgat gtacaagcgc gaggaggacg ggatcgtcct
     1141 ggtcgaccag gacgcctgcc gctcctggcg gttctgcgtc tccggctgcc cctacaagaa
     1201 ggtctacttc aactggaaca cgggcaaggc cgagaagtgc aacttctgtt acccgaggat
     1261 cgaggccggc atgccgaccg tctgctcgga aacctgcgtc ggccggctgc gtcacctggg
     1321 cgtcgtcctg atcgacaccg accgggtcga ggcggctgcc tcggtcgaga acgagcatga
     1381 cctgctcaac gcccaactcg acctgatgct ggatccggat gaccccgagg tccgcgagca
     1441 ggcactgcgg gacggcatcc cggccgactg gatcgacgcc gcttcgtact cgcccgtcta
     1501 cgcgatggcg aagaagtgga agatcgccct gccgctccac cccgagtacc ggacgctgcc
     1561 gatggtctgg tacgtgccgc cgctctcacc gatggagtcg gtcggcaagg cgctggtccg
     1621 cgatggcgaa gacgccgacg ccgagaatgt cttcgcggcg atcgacgaga tgcgaatccc
     1681 ggtcgagtac ctggcttcga tgctctcggc cggcgatgtc gagccggtc
//
LOCUS       DAOQFI010000497         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_45666_length_1725_cov_4.185629, whole genome shotgun sequence.
ACCESSION   DAOQFI010000497 DAOQFI010000000
VERSION     DAOQFI010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45666_length_1725_cov_4.185629"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLE93_08545"
     CDS             complement(<1..254)
                     /locus_tag="PLE93_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNH87138.1"
                     /translation="MTPYYDLLNKPCGGTFDDIVDSIGHTPLVELKTLSPKEDVRIFA
                     KLESANPTGSVKDRVARSMMQKAEEEGAIAEFLPPDGVLVL"
     gene            390..1250
                     /locus_tag="PLE93_08550"
     CDS             390..1250
                     /locus_tag="PLE93_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH87139.1"
                     /translation="MKKSRLIALLSALMILAAMVVVVGCGSSDDDSSSSDNGSTAASG
                     DFSSELINEGTLTVGTDTPYPPFEFGDAPDYNGFDVNLVDEIANRLGLETKWVDTSFD
                     TIFTDVAQGKFDMVASASTITPDRQKTVNFSDPYYSAQQALLVPEGSDISSVEDLDGK
                     TVGAQNGTTGKDFAENETNASRVQGYPNGPAAIEAVKNGQVEATIIDQPVAQDAIDKG
                     QTGFEVATTIPTGELYGFAFAKDTPELLAAVNKTLAEMKDDGSLDKIYQKYFSINAPE
                     EVVNGTTTNP"
     gene            1332..>1725
                     /locus_tag="PLE93_08555"
     CDS             1332..>1725
                     /locus_tag="PLE93_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH87140.1"
                     /translation="MGEFFQTYFDFSIMGDNFNAVLEGFWWTIKLSLIGGAFALAWGL
                     ILSVIRQLPGKALAPVRWLAIAYIDCLRGIPMLLTLLLIYGGLGTLQSTGTIPEWLGI
                     PEFFGWPSSFWYGVMALTLTYGAYMAEVY"
BASE COUNT          342 a          530 c          519 g          334 t
ORIGIN      
        1 agcaccaaca ccccgtccgg aggcagaaac tcggcgatcg cgccctcctc ctcggccttc
       61 tgcatcatcg accgggcgac ccggtccttg accgagccgg tcggattggc ggactcgagc
      121 ttggcgaaga tccgcacatc ttccttcggc gaaagcgtct tcagttcgac cagcggggtg
      181 tgaccgatcg agtcgacgat gtcgtcgaag gtgcccccgc agggcttgtt gagaaggtcg
      241 tagtaaggag tcatcttgtg ggcccgctgg acgggggtac ttcataaagt gtagtcactt
      301 ggacgccggt cggaagatct tcccggaaat cgtgaaatac tggccgggtt gcgcgtccag
      361 caaacaattc cctcctggag gaacctggca tgaagaaatc ccgattgatc gcgctgcttt
      421 cggcgctgat gatcctcgcg gcgatggttg tcgtggttgg ctgcggtagc agcgacgatg
      481 acagctcgag cagtgacaat ggcagcaccg ctgccagtgg cgacttttcg tccgaactga
      541 tcaacgaagg cacgctgact gtcggaaccg acaccccgta cccgcccttc gagttcggcg
      601 atgcgcctga ctacaacggc ttcgacgtga atctcgttga cgagatcgcc aatcgtctcg
      661 gcctcgaaac caagtgggtc gacacctcgt tcgacacgat cttcaccgat gttgcccagg
      721 gcaagttcga catggttgct tcggcttcca cgatcacgcc cgaccggcag aagacggtga
      781 acttctcgga cccgtactac tcggcccagc aggcgctgct cgtgcccgag ggttcggaca
      841 tcagttcggt tgaggatctc gacggcaaga ctgtcggcgc ccagaacggc accaccggca
      901 aggacttcgc cgagaacgag accaacgcca gccgtgttca gggttacccg aacggtccgg
      961 ccgcgatcga agccgtgaag aacggccagg tcgaagcgac catcatcgac cagccggttg
     1021 cgcaggacgc gatcgacaag ggtcagaccg ggttcgaggt ggcaacgacc atcccgaccg
     1081 gtgagctcta cggcttcgcc ttcgcgaagg acacgccgga gctgctggca gcggtcaaca
     1141 agacgctcgc ggagatgaag gatgatggct ctctggacaa gatctaccag aagtacttca
     1201 gcatcaacgc gcccgaagaa gtagtgaacg gaaccacgac caacccgtag ttccgaaggg
     1261 aatcgacaga aggggcgtcc ccgagacgcc ccttctgtcc ggttctcgaa caagtgacgg
     1321 gggagtatca gatgggtgag ttttttcaga catatttcga cttctccatc atgggggaca
     1381 acttcaacgc tgtcctcgaa ggtttctggt ggacgatcaa gctttccctg atcggcggag
     1441 ccttcgccct cgcgtggggc ctgatcctct ccgtcattcg ccagctgccg ggcaaggctc
     1501 tggccccggt caggtggctc gcgatcgctt acatcgactg cctccgcggc atcccgatgc
     1561 tgcttacgct gcttttgatt tacggcggtc tcggcaccct gcaatcaacc ggcacgatcc
     1621 cggagtggct gggaatcccc gagttcttcg gctggcccag ctcgttctgg tacggcgtca
     1681 tggcgctgac cctcacctac ggcgcctaca tggcggaggt ctacc
//
LOCUS       DAOQFI010000498         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46074_length_1717_cov_3.112515, whole genome shotgun sequence.
ACCESSION   DAOQFI010000498 DAOQFI010000000
VERSION     DAOQFI010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46074_length_1717_cov_3.112515"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLE93_08560"
     CDS             <1..552
                     /locus_tag="PLE93_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87141.1"
                     /translation="NIASAITLSDNDAAASLFTDLEGKHGGLEGASAAVGEMLRQAGD
                     RETIISTVGRDGFSTYGQTDWSLENQFRYMAALAGGCIGNAGNRAYLLDQMARVGGSD
                     TFGLGAAGFPARWKGGWGPGTDGKYLVRQMGVMVVNGKQVVVSMAAIPDDGTFESGAS
                     MLSAIARSVASRLAGRVSAPTGC"
     gene            602..1051
                     /locus_tag="PLE93_08565"
     CDS             602..1051
                     /locus_tag="PLE93_08565"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH87142.1"
                     /translation="MDRETVIRELRLEPLPKEGGMYRRKFKDDNSSSIYFLIEPDSPT
                     NLHRLPWPELFHFYAGAPARLHLLGPEPGEARHPVLGIGLEEGERPQMLVPAGTWQAA
                     ETTGAWTLLGTTMAPPFDWDRFELGKRERLLKYWPDQREVIERHLAT"
     gene            complement(1061..>1717)
                     /locus_tag="PLE93_08570"
     CDS             complement(1061..>1717)
                     /locus_tag="PLE93_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH87143.1"
                     /translation="EIIRAFEAEFNTPILEGYGLSETSPVACFGRVDMERKAGTIGTP
                     IRDVELRLVDEEGNVLGVGDVGELQIKGPNVMKGYWQMPEATEKAIDADGWFASGDMA
                     TVDEDGYYSIVDRKKEMILRGGYNVYPREVEEVLYEHPAVAEAAVMGVPHDELGEEIV
                     AIIAFKEGESATEDELRDHAKEGVAAYKYPRHILIVDELPKGPTGKILKREIDLSQVG
                     "
BASE COUNT          293 a          578 c          548 g          298 t
ORIGIN      
        1 aacatcgcga gcgcgatcac cctctccgac aatgacgcgg ccgcctcgct ctttaccgac
       61 ctggaaggaa aacacggcgg cctcgaaggg gcctcggcgg cagtcgggga aatgctccgg
      121 caggccggtg accgggaaac catcatttcg acggtgggcc gggacggttt ctcgacctat
      181 ggccagaccg actggtcgct ggaaaaccag ttccggtaca tggcggcact ggccggaggc
      241 tgcatcggca acgcgggcaa ccgcgcctac ctgctggacc agatggcccg ggtcggcggc
      301 agcgacacct tcgggctcgg cgcagccggc ttccccgcga ggtggaaggg aggctggggc
      361 ccgggtaccg acggcaaata cctggttcgc cagatgggcg tgatggtcgt gaacggcaaa
      421 caggtcgtgg tcagcatggc cgcgattccg gatgatggaa ccttcgagtc cggcgcgtcg
      481 atgctgagcg cgatcgcgcg atccgtcgcc tcgcgcctgg ccggccgcgt ttccgccccg
      541 actggctgct gactgtctgc cagccggggc aaaccggagc aaactggcgt atcgtccggg
      601 tatggatcgc gaaacggtaa tccgcgagct gaggctcgag ccgctgccca aagagggcgg
      661 catgtaccgg cgcaagttca aggacgacaa ctccagctcg atctactttc tgatcgaacc
      721 ggattcaccg accaaccttc accggctccc ttggcctgag ctctttcact tttacgccgg
      781 cgccccggcc cggctgcatc tgctcggtcc cgagcccgga gaagcgagac acccggtgct
      841 cggcatcggg ctcgaagagg gcgagcgtcc gcagatgctc gtgcccgccg gaacctggca
      901 ggcggccgag acgaccggcg cctggaccct gctcggcacg accatggcgc cgcccttcga
      961 ctgggaccgc ttcgaactag gcaagcgcga gcgcctgctc aagtactggc ccgatcagcg
     1021 cgaagtgatc gaacgccacc tcgccacctg agcaccaggc tcagccaacc tggctgagat
     1081 cgatttcgcg cttcaggatc ttgccggtgg ggcccttggg gagctcgtcg acgatcagga
     1141 tgtggcgcgg gtacttgtag gcggcgacgc cttccttcgc gtggtcgcgc agctcgtctt
     1201 cggtcgccga ctcgccctcc ttgaaggcga tgatcgcgac gatctcctca ccgagttcat
     1261 cgtgggggac gcccatcacg gcggcctcgg ccacggccgg atgctcgtag agcacctctt
     1321 cgacctcgcg gggatagacg ttgtaaccgc cgcgcaggat catctccttc ttgcggtcga
     1381 cgatcgagta gtaaccgtcc tcgtcgacgg tcgccatgtc gcccgaagcg aaccagccgt
     1441 cggcatcgat cgctttctcg gtcgcttccg gcatctgcca gtagcccttc atcacgttcg
     1501 gacccttgat ctgaagctca ccgacatcgc cgactccgag cacattgccc tcctcgtcga
     1561 ccagccgcag ttccacatcc ctgatcgggg tgccgatcgt gccggccttt cgctccatgt
     1621 cgacccggcc gaagcaggcg accggagaag tctcggagag gccgtaaccc tcgaggatcg
     1681 gcgtgttgaa ttcggcctcg aaggcgcgga tgatctc
//
LOCUS       DAOQFI010000499         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46163_length_1715_cov_3.174096, whole genome shotgun sequence.
ACCESSION   DAOQFI010000499 DAOQFI010000000
VERSION     DAOQFI010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46163_length_1715_cov_3.174096"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1397)
                     /locus_tag="PLE93_08575"
     CDS             complement(12..1397)
                     /locus_tag="PLE93_08575"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNH87144.1"
                     /translation="MSTEQQTEAPAEGDGGGVAAGNDTLSVTDNRTGRTYELPIEDDT
                     IKALDLRQIKVHEDDFGMMAFDPAFTNTASCRSSITYIDGDAGILEHRGIPIDQLCEH
                     STFLEVAYLLVFGELPTRPQLEAWVHDVTHHTFVHEDIKKFLTGFRYDAHPMGMLLAS
                     TGALSTFYPDSNQISDPVERYLATIRLIAKMPTLAAFSYRHNMGLPYVYPDNDLSYPE
                     NFLSMMFKMTEAKYEPDPRLSKAIDVLFILHADHEQNCSTSAVRGVGSSDVDPYSAVA
                     AGIAALYGRLHGGANEAVLQMLRRIEKVDNIPGFLEAVKNREEKLMGFGHRVYKNYDP
                     RARIIKSHADDVFEATEYNPLVEIATELEKQALDDEYFTSRKLFPNVDFYSGIIYEAL
                     RIPPGMFTVIFAIARTAGWISQWLEMTDDPDKKIARPRQIYTGAREANYVPVGERTGE
                     DRISGPAIRPS"
     gene            complement(1453..>1715)
                     /locus_tag="PLE93_08580"
     CDS             complement(1453..>1715)
                     /locus_tag="PLE93_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH87145.1"
                     /translation="SLKAHRVDVVDPTGAGDSFAGGFLGYLDGEAGGIDQQTLRTAMG
                     YGTVLASYNVEEFGTERVSRLSRDEIESRLEALRSMTSFSLT"
BASE COUNT          293 a          482 c          599 g          341 t
ORIGIN      
        1 cgacaggcga ctcaggaggg gcggatggcc gggccggaga tgcggtcctc gccggtgcgc
       61 tcgcctacgg gcacgtaatt tgcctcgcgg gcgccggtgt agatctggcg cggccgggcg
      121 atcttcttgt ccgggtcgtc ggtcatctcc agccactggg agatccaccc ggcggtgcgg
      181 gcgatcgcga agatcaccgt gaacatgcct ggcgggatcc gcagggcctc gtagatgatc
      241 ccggaataga agtcgacgtt cgggaagagc ttgcgcgaag tgaagtactc gtcgtcgaga
      301 gcctgctttt cgagctcggt cgcgatctcg accagcgggt tgtactcggt cgcttcgaag
      361 acgtcgtcgg cgtgactctt gatgatccgg gcgcgcgggt cgtagttctt gtagacccgg
      421 tgaccgaagc ccatcagctt ctcttcacgg ttcttcacgg cttcgaggaa gccggggatg
      481 ttgtcgacct tctcgatgcg gcggagcatc tgcagcactg cctcgttggc gccgccgtgg
      541 aggcgaccgt aaagggcggc gatgccggct gcgaccgccg agtagggatc cacgtcggac
      601 gagccgactc cacgcacggc gctggtcgag cagttctgct cgtggtcggc gtgcaggatg
      661 aagaggacgt cgattgcctt ggagagccgg gggtccggct cgtacttcgc ctcggtcatc
      721 ttgaacatca tcgagaggaa gttctccggg taggagaggt cgttgtccgg atagacgtaa
      781 ggcagaccca tgttgtgccg gtaggagaaa gcggccaaag tcggcatctt ggcgatcagg
      841 cggatcgtcg ccaggtagcg ctcgaccggg tcactgatct gattcgaatc agggtagaag
      901 gtcgaaagcg caccggtcga ggcgagcagc atgcccatcg ggtgggcgtc gtagcggaag
      961 ccggtgagga acttcttgat gtcctcgtga acgaaggtgt gatgggtgac gtcatggacc
     1021 caggcttcga gctgcgggcg tgtgggaagc tcgccgaaga ccagcaggta agccacttcg
     1081 aggaaggtcg agtgctcaca aagctggtcg atcgggatac cgcggtgctc gaggattccg
     1141 gcatcaccgt cgatgtaggt gatcgaggag cggcaggagg ccgtattggt gaaggccgga
     1201 tcgaaggcca tcatcccgaa gtcgtcttcg tggaccttga tctggcgcag atccagcgcc
     1261 ttgatcgtgt cgtcctcgat cggaagctcg taggtgcgac cggttcggtt gtcggtgacc
     1321 gagagggtgt cgtttccggc ggctactccg cccccatcgc cctcggcggg cgcttcggtc
     1381 tgctgttcgg tcgacatgct tctcctcgga tcctgttctc tgatctttct tcggaccagt
     1441 atctcaggtc ggttaagtca gcgagaaact ggtcatcgac cgcagcgctt cgaggcgcga
     1501 ctcgatctcg tcgcggctca ggcggctgac tctttcggtg ccgaactcct cgacgttgta
     1561 ggaggcgagc acggtgccgt agcccatcgc ggtgcgcaga gtctgctggt cgatgccgcc
     1621 ggcctcgccg tcgaggtaac cgaggaagcc gccggcgaag gaatcaccgg cgccggtcgg
     1681 gtcaacgaca tcgacgcgat gggctttcaa cgaca
//
LOCUS       DAOQFI010000500         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46187_length_1715_cov_2.309036, whole genome shotgun sequence.
ACCESSION   DAOQFI010000500 DAOQFI010000000
VERSION     DAOQFI010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46187_length_1715_cov_2.309036"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..1436)
                     /locus_tag="PLE93_08585"
     CDS             complement(285..1436)
                     /locus_tag="PLE93_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HNH87146.1"
                     /translation="MASIEQIIEGREIAICAGSGGVGKTTTSAAIALGMAARGYKVCV
                     LTIDPAKRLADSLGLKELGNEASKVNPKLLKEQGVDPESGGELWAMMLDAKETFDALV
                     ARHAEDAEARDRVLNNRIYQQISGALAGSQEYMAMEKLFELHSEGRFDLLVLDTPPSR
                     NALDFLDAPQRLTQFIEGKSLQVFIKPTGLAARVASRGTGVALSVLKRIVGFDLLADL
                     SEFFTAFSGMIDGFQDRAHRVSSLLADERTSFLIICGPQSEPIDEAVFFHSKLVENRM
                     NFGGAVVNRVRYRLGSHQVKVKDLSGRIDRELDDTALAERLARNLAEYDVLAVRDQKN
                     IDELTRALNGEPVIRIPYLDTDVHDIAGLCQINEYLFVEEDERVEKAIS"
     gene            complement(1436..>1715)
                     /locus_tag="PLE93_08590"
     CDS             complement(1436..>1715)
                     /locus_tag="PLE93_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87147.1"
                     /translation="GIAIDRVVMNALYPETFSPSEVKKLRKLKKSDDAEVRAAGRAAA
                     SQATRAAAQREQLARLEEMVETEITTLPFIFKPELDLKSARKLAEGIT"
BASE COUNT          287 a          546 c          546 g          336 t
ORIGIN      
        1 cgcgcgagac cccgattctc agccgttagg gcacccaact ccgatctcga ccgcctcccg
       61 atcccaaccc ggggcaggtg cctatggatt tcgtctatat agacgaaatc catagccacc
      121 tcctgaacct gggcgtgaac ccgactctgg cgccgacacg ggactcgccc agggtccatc
      181 gagatgcgct tttctcccgg tatagggcag aaaagcgcat ctcgcgtttc cgggatcggg
      241 gagccgcgaa cgagttggcg taaccagcag gtcagtccga gtgatcagga gatggctttt
      301 tcgactcgtt catcctcttc gacgaagagg tactcgttga tctggcagag gccggcgatg
      361 tcgtggacgt cggtgtcgag ataggggatc cggatcaccg gctctccgtt gagggctctg
      421 gtcaactcgt cgatgttctt ctggtcgcgg accgcgagca cgtcgtattc ggcgaggttg
      481 cgggccagtc gctccgcgag cgcggtgtca tccagctcgc ggtcgatccg cccgctcagg
      541 tccttgacct tcacctgatg gctgccgagc cggtagcgga cccggttgac cacggcgccg
      601 ccgaagttca ttcggttttc gaccagcttc gaatggaaga agaccgcctc gtcgatcggt
      661 tcgctctggg gtccgcagat gatcaggaag cttgtccgct catcggccag cagtgaactg
      721 acccgatggg cccggtcctg gaaaccgtcg atcatcccgc tgaaggcggt gaagaattcg
      781 gagaggtcgg cgagcaggtc gaagccgacg atccgcttca gcaccgaaag tgccaccccg
      841 gtgcctcggc tggcgacccg ggcggcaagc ccggtcggct tgatgaagac ctgaagcgac
      901 ttgccctcga tgaactgggt caggcgctgc ggcgcgtcaa ggaaatcaag cgcgttccgg
      961 ctcggcgggg tatcgagcac cagcaggtcg aagcggccct ccgaatggag ctcgaagagc
     1021 ttctccatcg ccatgtactc ctgcgaaccc gccagggccc cggagatctg ctggtagatc
     1081 cggttattca gcacccggtc ccgggcttcg gcatcttccg cgtggcgagc gaccagggcg
     1141 tcaaaggttt ccttggcgtc gagcatcatc gcccagagtt caccgccgct ctccggatcc
     1201 acaccctgtt ccttcagcag cttcgggttc accttgctgg cctcgttgcc cagttccttc
     1261 aggcccagtg aatcggccag ccgcttggcc gggtcaatgg ttagcacgca taccttgtag
     1321 ccccgggccg ccatgccaag ggcgatcgcg gccgaggtgg tcgtcttgcc gacgccgccc
     1381 gatccggcac agatcgcgat ttcgcggccc tcgatgattt gctcgatcga ggccatcagg
     1441 tgatcccctc ggccagtttg cgggccgact tcaggtcgag ttcaggcttg aagatgaagg
     1501 gaagggtggt gatttcggtc tcgaccatct cctcgagccg agcgagctgc tcgcgctggg
     1561 cggcggcccg ggtggcctgg gaggcggcgg ctctgcccgc ggcacgcacc tcggcgtcat
     1621 cgctcttctt cagcttgcgc agcttcttga cctcggacgg ggagaaggtc tcggggtaga
     1681 gcgcgttcat gaccacccgg tcgatcgcga taccg
//
LOCUS       DAOQFI010000501         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46220_length_1714_cov_3.162749, whole genome shotgun sequence.
ACCESSION   DAOQFI010000501 DAOQFI010000000
VERSION     DAOQFI010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46220_length_1714_cov_3.162749"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..813
                     /locus_tag="PLE93_08595"
     CDS             88..813
                     /locus_tag="PLE93_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH87148.1"
                     /translation="MTATPAISIRDLRVSRGGNLVLPDISVEIEKGSVTGLLGPSGCG
                     KTTLMRSIVGVQIVAGGTVEVLGRPAGSSDLRNRVGYVTQAPSVYGDLSVGENLDYFA
                     RVVGVSPERVAETIETVGLSGFTGRLVSSLSGGQRARVSLASALLNKPDLLVLDEPTV
                     GLDPLLRAELWKTFHELAESGTTLLVSSHVMDEAGACDHLILIRDGRLLRHTTPEALR
                     RETGENDLGRAFLKVIESDEEAA"
     gene            810..1547
                     /locus_tag="PLE93_08600"
     CDS             810..1547
                     /locus_tag="PLE93_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH87149.1"
                     /translation="MSPRITLATGLRVLQQIRHDPRTIALLLLVPALLLTLMKYVFES
                     EPRVFQQIGAPLCGLFPFIVMFLVTSITMLRERTTGTLERLMTLPMSKLDLLLGYALA
                     FGLLAAAQALVICLVGFGLLGLNAPHGVLIIVGLAVLNALLGMSLGLLVSAFANTEFQ
                     AVQFMPAFIFPQLLLCGLFVARDQMAPLLEAVSWVMPFTWAYDALARAVSPQPLSGRV
                     AVDAAVVLAVTIGSLIFGAMTLRRRTA"
BASE COUNT          252 a          594 c          557 g          311 t
ORIGIN      
        1 ccgggaaatg ccgctcgttg acggcgagca gacgagggcc gccgccgcgg cattcgccgc
       61 cgccctggcc gaagacctcg aagctcaatg acggcaacgc cggccatcag catccgcgac
      121 ctgcgcgttt cgcgcggggg gaaccttgtc ctgcctgaca tctcggtcga gatcgagaag
      181 ggatccgtga ccgggctgct cggcccgagc ggatgcggga agacgaccct tatgcgttcg
      241 atcgtgggag tccagatcgt ggccgggggc acggttgaag tgctcggccg gccggccggg
      301 tccagtgacc tgcgcaaccg ggtcggttac gtgacccagg cgccctcggt ctacggggac
      361 ctttccgtgg gcgagaatct cgactacttc gcgagagtgg tcggggtctc cccggaacgg
      421 gtcgcggaga ccatcgaaac ggtcggcctc tccgggttca ccggaaggct ggtctcctcg
      481 ctctcgggag ggcaacgggc ccgggtttcg ctggccagcg ctctcctcaa caaacccgac
      541 ctgctggtgc tcgacgagcc cacggtcggc ctcgatcccc tgctccgggc cgaactctgg
      601 aagaccttcc atgaactggc cgaaagtggc accaccctgc tggtttccag tcacgtcatg
      661 gacgaggccg gcgcttgcga tcacctgatt ctgattcgcg acggacgcct gctgcgccac
      721 acgacgcccg aagcactgcg ccgggaaacc ggcgagaacg acctcggccg cgccttcctc
      781 aaggtgatcg agtcggacga ggaggcggcg tgagtccgcg catcaccctg gccaccggcc
      841 tcagggtcct gcagcagatc cggcacgatc cccggacgat cgccctcctg ctgctcgttc
      901 cggcccttct cctcaccctg atgaagtacg tgttcgaaag cgaacccagg gtctttcagc
      961 agatcggcgc tccgctctgc ggcctgtttc ccttcatcgt catgttcctg gtgacctcga
     1021 tcacgatgct gcgggaacga accaccggaa cgcttgagcg gctgatgacc ctgccgatgt
     1081 caaagctcga cctgctgctc ggctacgcgc tggccttcgg gctgctcgcg gcggcccagg
     1141 cgctggtcat ctgcctggtc ggattcggcc tcctgggcct gaacgccccc cacggcgtcc
     1201 tgatcatcgt cgggctggca gtcttgaatg ccctgctcgg gatgtcgctc gggctgctcg
     1261 tcagcgcctt tgcgaacacc gagttccagg cggtccagtt catgccggcg ttcatctttc
     1321 cccagctgct gctctgcgga ctgttcgtgg cccgcgacca gatggcgcct ctgctcgaag
     1381 cggtctcctg ggtcatgccc ttcacctggg cctatgacgc cctggcccgg gccgtttccc
     1441 ctcagccgct cagcggccgg gtcgccgtcg acgccgcggt cgtactcgcg gtgacgattg
     1501 gatcactgat tttcggcgcc atgaccctcc gccggcggac tgcctagaaa gtcgcgtcgc
     1561 ggaggtacca ctcggcgtcg agggcgccct tgcagccgga gccggcggcg gtgaccgcct
     1621 ggcggtaggt gtggtcaacc aggtcgccga tcgcgaagac cccggccagg ttggtcttgg
     1681 tcgaggcggc ctcgaccaag accaacctgg ccgg
//
LOCUS       DAOQFI010000502         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46278_length_1713_cov_2.681544, whole genome shotgun sequence.
ACCESSION   DAOQFI010000502 DAOQFI010000000
VERSION     DAOQFI010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46278_length_1713_cov_2.681544"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PLE93_08605"
     CDS             <1..964
                     /locus_tag="PLE93_08605"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH87150.1"
                     /translation="GSEVTALTRDLVRRGLDTNALEPYRAGQNVTWQKWMEAAFDETD
                     DPDELRELLEVSARSIFAYVDSTMMLTADRIQQQREEFNQTGAERLETVVLLLEDNPI
                     DPHRAAARLNYSFEGTQLAAVIWKEETPDADALEVGAERLKQAAGSAKLLAVHATSAA
                     LWAWVKPTREFSEIEAGLAGDGQIRFALGTPLPGLDGFRRSHADASEVQRLVLRLDSG
                     LEVASYEAMKVAALLTHDEERSKRFTAETLGELADGPVNLKETVRTYLRNQSNATRTA
                     ELLFAHRNTVLARIAKADELLPSPLSESSFEVAAALEILHWQES"
     gene            complement(993..>1713)
                     /locus_tag="PLE93_08610"
     CDS             complement(993..>1713)
                     /locus_tag="PLE93_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNH87151.1"
                     /translation="KSEAECRAMAGFPPPAAKVVAGFEKVTGKTFPKLKPNRRFYEKP
                     TFYVANHAAVIADGDPMWWPSHTKFLDFELELAFVIGKPIADASPEEAADAVGGWFVL
                     NDWSARDVQADDARENIFGPVVKSKTFANSIGCDVITPDELPDWQEATGRVRVDGELW
                     CEGTTAGAAHDIGAMLAYASAGECLMPGDVISTGTVPGCCGLELDRWIQPGQTVELEI
                     DGIGTLTNRIDAEARPVSIAG"
BASE COUNT          299 a          582 c          549 g          283 t
ORIGIN      
        1 cggctcggag gtcaccgcgc tgacccggga cctggttcgc cggggactgg acaccaacgc
       61 cctcgagcct taccgggccg gtcagaacgt cacctggcag aagtggatgg aagccgcctt
      121 cgacgagaca gatgatccgg acgagttgcg ggagctgctt gaagtctcgg cccgctcgat
      181 ctttgcctat gtggactcga ccatgatgct taccgcggac cggatccagc agcagcggga
      241 ggagttcaac cagaccggcg ccgagcgact ggaaacggtg gtgctcctgc ttgaggacaa
      301 cccgatcgat ccccatcgcg cggcggcacg cctcaactac agcttcgagg ggacccagct
      361 cgccgccgtg atctggaagg aggaaacgcc ggacgccgac gcgctcgaag tcggggcgga
      421 gaggctgaag caggccgccg gttccgcgaa gctgctggcc gtgcacgcga ccagcgctgc
      481 cctctgggcc tgggtcaagc caaccaggga gttcagcgaa atcgaggcgg gtcttgccgg
      541 cgatggccag atccgcttcg cgctcggcac acccctcccc ggtctggacg gcttccggcg
      601 cagccacgcc gatgcctccg aggttcagcg gctggtgttg cggcttgatt ccggccttga
      661 ggttgccagc tacgaggcga tgaaggtggc cgccctgctg actcacgacg aagaacggtc
      721 gaagcggttc accgcggaga ccctcggcga gctggccgac ggccccgtca acctcaagga
      781 gacggtgcgc acctacctcc ggaaccagtc aaatgcgacc aggacggcgg aactgctctt
      841 cgcgcaccgg aacacggtcc tggcgaggat cgccaaggcc gacgaactgc tcccctcccc
      901 gctctcggaa tcctcgtttg aagtcgccgc cgccctggag atccttcact ggcaggagag
      961 ctgaccccgc ctgaaggcct gtcgcgaccc gcctatccgg cgatcgagac aggcctcgct
     1021 tcggcgtcga tccggttggt gagggtgccg ataccgtcga tttccagctc cacggtctgg
     1081 cccggctgaa tccagcggtc gagttcaagc ccgcaacagc ccggcacggt gccggtcgag
     1141 atcacgtcgc caggcatcag gcactcaccg gcggaagcgt aggcgagcat cgctccgatg
     1201 tcatgagccg ccccggcagt cgtaccctcg caccacagtt caccgtcgac ccggacccgg
     1261 cccgtagcct cctgccagtc cggcagttcg tccggggtga tcacgtcgca gccgatcgag
     1321 ttggcgaagg tctttgactt caccaccgga ccgaagatgt tctctcttgc gtcgtcggcc
     1381 tgaacgtctc gggcactcca gtcgttcaga acgaaccagc caccgacggc atcggcggcc
     1441 tcctccgggc tggcatccgc gatcggcttg ccaatcacga acgcaagctc gagttcgaag
     1501 tcgaggaact tcgtgtggct gggccaccac atcgggtcgc catcggcgat cacggcggcg
     1561 tgattggcca catagaaggt cggcttctcg tagaagcgcc tgttcggttt cagcttcggg
     1621 aaggtcttgc ctgtgacctt ctcgaaacct gccacgacct tggcggccgg cggcgggaag
     1681 cccgccatgg cgcggcactc cgcctccgac ttg
//
LOCUS       DAOQFI010000503         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46318_length_1712_cov_3.214243, whole genome shotgun sequence.
ACCESSION   DAOQFI010000503 DAOQFI010000000
VERSION     DAOQFI010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46318_length_1712_cov_3.214243"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1268)
                     /locus_tag="PLE93_08615"
     CDS             complement(240..1268)
                     /locus_tag="PLE93_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87152.1"
                     /translation="MFKVARKVVLAMLLVATLLIPAMAQAAGPPPLPKASNGAKVTRF
                     ATGVPVPTQVAFSGRNAFVAGATEGPFKGGLFVVKPGSKKAVKVPGTVKGAFGIAARK
                     GKVFVSSGRKIVAFARFDGKRFRARKVIFAGPKNFTGFSGLAIGPNGRLYAGVSLNQK
                     HDHTADPSLYGNSVVSMGLAGKNVKVVATGLRQPWMMTFAPGVRDPFVSVLGPDTPVN
                     NGAPDLIVKARPGSDFGFPECDWLIEDECDGLAEPLVRLEAQPGKKGSLNQQSPMGIG
                     AIGKRLYVALFSGTAKAGIQVMTMKTDGSGIKPFMTGFVAPVLSVATHKGYVYAGDLT
                     GTIYKVKG"
     gene            complement(1379..>1712)
                     /locus_tag="PLE93_08620"
     CDS             complement(1379..>1712)
                     /locus_tag="PLE93_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87153.1"
                     /translation="PGALDNGDYVYLPGVRKALRLKVSCDEACSGKVTVTGKGVKFAG
                     RVYLTRAGVAKLKLRPTVKARRVLARLATRKPRSTGARSLGLTARASLKDAAGNTGKA
                     SLRFKVTA"
BASE COUNT          267 a          551 c          599 g          295 t
ORIGIN      
        1 gcgacaccgg tcgcgaagac cttgaccggc ttgccgtttt cggcaaccgg caccccgggc
       61 gctgccgcag tcgcggtgtg ggccatcgcg gccaatccga tcaaggcagc tgctagcgcc
      121 gccaatgaac ccctgagctt catcattcgg tctcactttc cccggttttg gatccagcca
      181 acccacctta cctgccgcgg acacccgcaa caagaaggtg ggttggtgga ctccggggac
      241 tagcccttga ccttgtagat ggtgccggtg aggtcgccgg cgtagacgta acccttgtgg
      301 gtagcgaccg agaggaccgg agcgacgaag ccggtcatga acggcttgat gccgcttccg
      361 tcggtcttca tcgtcatcac ctggatgccg gccttggcgg tgccgctgaa gagggcgacg
      421 tagagccgct tgccgatcgc gccgatgccc atcggggatt gctggttcaa ggagcccttc
      481 ttgccgggct gggcctcgag ccggaccagc ggttcggcca ggccgtcgca ttcatcctcg
      541 atcagccagt cgcactccgg gaagccgaag tcggaaccgg gccgggcctt gacgatcagg
      601 tccggggcgc cgttgttgac cggcgtatcc ggtccgagga ccgagacgaa ggggtccctg
      661 accccggggg cgaaggtcat catccacggc tgcctgagcc cggtcgcgac caccttcacg
      721 ttcttgccgg ccagccccat cgagacgacc gagttgccgt agagcgaggg gtcggcggtg
      781 tggtcgtgct tctggttgag cgagacgccg gcgtagaggc gtccgttcgg gccgatcgcc
      841 agtccgctga agccggtgaa gttcttcggt ccggcgaaga tcaccttgcg ggcgcggaag
      901 cgcttgccgt cgaatcgggc gaaggcgacg atcttgcggc cggagctgac gaagaccttg
      961 cccttgcgcg cggcgatccc gaacgcgccc ttgacggtgc cgggaacttt cacggccttc
     1021 ttcgaaccgg gcttgacgac gaagagaccg cccttgaacg gaccctcggt cgcgccagcc
     1081 acgaaggcgt tccggccgga gaaggcgacc tgggtcggga ccggcacgcc ggtggcaaag
     1141 cgtgtcacct tggcgccgtt cgacgccttg ggcagcggcg gcgggccggc cgcctgggcc
     1201 atggcgggga tcagcagggt ggcgacgagg agcatcgcca gaactacttt tcgagctact
     1261 ttgaacattg cttcctttgt gttgttgagg ggagataact gccgctacgg ggagcgacgg
     1321 attgattccg ggggcgatcg gccaccaaat cggtggccga tcgcccccgg aagggacttc
     1381 aggcagtgac cttgaaccgg agcgaggcct tgccggtgtt gccggcggcg tccttcaggc
     1441 tggcccgtgc ggtcagcccc agggaccggg ctccggtgct ccgcggtttg cgggtggcga
     1501 gccgggccag gacacgcctg gccttcacgg tcgggcgcag cttgagcttc gccaccccgg
     1561 cccgggtcag gtagacgcgg ccggcgaact tcacgccctt gccggtgacc gtcaccttgc
     1621 ccgagcaggc ctcatcgcag ctgaccttga gacgcagcgc cttgcggacg ccgggcaggt
     1681 agacgtaatc gccgttgtcc agcgcgccgg gg
//
LOCUS       DAOQFI010000504         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46526_length_1708_cov_3.470054, whole genome shotgun sequence.
ACCESSION   DAOQFI010000504 DAOQFI010000000
VERSION     DAOQFI010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46526_length_1708_cov_3.470054"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLE93_08625"
     CDS             <1..655
                     /locus_tag="PLE93_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HNH87154.1"
                     /translation="LGALAEIGVPLIVSVMADSAEDFATLVERVAARDEVAGIELNVS
                     CPNVHSGLIVGEQPAETEALMKALRPLTGKPLIVKLTPNVANPQEVAVAAEAGGADSV
                     SMINTIKAAAIDPASSRTWLGAGAGGLSGPAVRPIALAQVRAVSSAVGIPVVGMGGIS
                     DAESALEFILAGAEVIAVGTENFRNPRAGAAVTEGLPLALRRRGFGSLAEARAATIG"
     gene            822..1163
                     /gene="mihF"
                     /locus_tag="PLE93_08630"
     CDS             822..1163
                     /gene="mihF"
                     /locus_tag="PLE93_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HNH87155.1"
                     /translation="MAPTSKTVSSPPEAPERSLDQRMDALKRANNIRTARAQLKRDLK
                     AGRTTITEVLDEPPEYLLTAKVFDMLLAVPKYGRVKTNRILKQCKVSPAKTIGGLSAR
                     QRQELVSLLQR"
BASE COUNT          311 a          582 c          551 g          264 t
ORIGIN      
        1 cctaggggcc ctggccgaaa tcggggtgcc cctgatcgtt tcggtcatgg ccgacagcgc
       61 cgaagacttc gccaccctcg tcgagcgggt cgcggccagg gatgaggtgg ccgggatcga
      121 actcaatgtt tcctgcccca acgtccactc cggactgatc gtcggcgagc agcccgccga
      181 gaccgaggcc ctgatgaagg cgctgcggcc gctgaccggc aagccgctga tcgtcaagtt
      241 gacgcccaac gtggccaacc ctcaagaggt ggctgttgcg gccgaggccg ggggagccga
      301 ctcggtctcg atgatcaaca cgatcaaggc ggccgcgatc gacccggctt ccagccgaac
      361 ctggcttggc gccggtgccg gcggactctc ggggccggcg gtcaggccaa tcgccctggc
      421 ccaggtgcgg gcggtttcat cagcggtcgg aatcccggtg gtcggcatgg gcggcatcag
      481 tgacgccgag tcggcactcg agttcatcct ggccggcgcc gaggtgattg cggtcggtac
      541 cgagaacttc cgcaaccccc gggccggggc ggccgtgacc gagggtcttc cgcttgctct
      601 ccggcgccgc gggttcggca gcctggccga ggcccgggcg gccacgatcg gctgagccgg
      661 ctcttaccga acgggcgccc gccggacatg cccggccggt gaccggttcc gcctcgccct
      721 caacctcgag tcgaggttga gatggatttg cccgaaatcg gcacttttcg ggggttttcg
      781 gcctccaaac ttctcgccag tggctatact cgccgccttc catggccccg acctcgaaga
      841 ccgtatccag cccgccggaa gccccggaaa ggtccctcga ccagaggatg gacgcactca
      901 agagggccaa caatattcgc accgccaggg cccagctgaa gcgggacctc aaggccggtc
      961 gcaccacgat caccgaggtt ctcgacgagc cgccggaata cctcctgacc gccaaggtct
     1021 tcgacatgct gctcgccgtt ccgaagtacg gcagggtcaa gaccaaccgg atcctcaagc
     1081 agtgcaaggt cagtcccgcc aagaccatcg gcggactcag cgcccgccag cgccaggaac
     1141 tcgtaagcct cctccagcgt taacccgggc aagcaccagc ctcaacccag gcaggcacca
     1201 gcctcaaccc gggtaagcac cagctcaaac ccgggcaggc agcaagacag aacccgcggc
     1261 aagcagggct ctcccgagat ccggttttcc gccaaatacc gcacccaact ccgatctcgc
     1321 ccgttcccca ctcatgatcc gtgaagggtg ccgcaggttt gcgcccacag aggtaacaaa
     1381 cctgctgtac cgaccaaaga cgaggccgaa tacccaaccc tggaggcggc aagcagctgc
     1441 gggccacctg aattcccccg tcacttgccc tgttccaccc gggtgggtcg gataaccggt
     1501 acctgggccg gaaaaccgac ccacgagtct tccgaaccgg accgggcgtt ggggtgggtc
     1561 gggtttccgg ggctggtgac ggaaatccgc cccagcggca tcgaggcacg gtccgggttg
     1621 atcgagatct gggttggggg cggtatttgg cagaaagtgg gatctcgcga aagacaggct
     1681 ggggttgcgt tggcttccgg agtggcgg
//
LOCUS       DAOQFI010000505         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46601_length_1707_cov_2.314165, whole genome shotgun sequence.
ACCESSION   DAOQFI010000505 DAOQFI010000000
VERSION     DAOQFI010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46601_length_1707_cov_2.314165"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PLE93_08635"
     CDS             <1..551
                     /locus_tag="PLE93_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87156.1"
                     /translation="PDVVQFLSDHAGKAEVFVDLGSGDGFFCAGVARKASPEVIGFET
                     NQFECKLAHELARKNGVQIETRGLATEDGLNDLPRGRLLLLSDVEGLEEDLIDPEIVP
                     RLREATMVIETHEQFRPNVIEKLNERFSDTHDISRIFKEVPGRKSCPEVKDWSDGDFE
                     IAAYDGHHSGDSWMTLVPKKPG"
     gene            621..1091
                     /locus_tag="PLE93_08640"
     CDS             621..1091
                     /locus_tag="PLE93_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNH87157.1"
                     /translation="MNRREQIEMTQEEAEAFLLERKVAVVTSFGPRGWPHSMPLWYVP
                     NGDRIRSWTFAKSQKVKNLERDPRATVLVEDGYDNYSELRGVMMETETLIHRDPNLVT
                     AYAESSVARYTPEGVELTTEMLEAFRSQAPKRVVLEFIPVRVLTWDHRKLGGTY"
     gene            complement(1137..>1707)
                     /locus_tag="PLE93_08645"
     CDS             complement(1137..>1707)
                     /locus_tag="PLE93_08645"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH87158.1"
                     /translation="VDSAGIRCDRTLTAWDHLTGEPATRLEVASDPLGPTGGAALFDR
                     VAFETIGGFDERIFLYYEDLDLALRMRMAGYSCRLARAARATHESSATLGRRAAAKYR
                     RTGFSRGYLLRKYGIMAHPGLAIMTLISDGSASLAQLLHDRTAAGLNGRIEGWQAGRD
                     IVPIDPPFELLEPPSLRRHMRVRLNRRRY"
BASE COUNT          372 a          464 c          540 g          331 t
ORIGIN      
        1 aaccggatgt tgtgcagttt ttgagcgatc atgccggaaa ggcggaagtg ttcgtcgatc
       61 tcggatccgg ggacgggttt ttctgcgcag gcgtggctcg aaaggcttcg ccagaggtga
      121 tcggctttga aacgaatcag ttcgaatgca agttggcaca cgagttggcc agaaagaacg
      181 gggttcagat cgaaacccgc ggattggcaa ctgaagacgg gctgaacgat ctccccagag
      241 gaaggctgct cctgctttcc gacgtagagg gacttgagga ggatctcatc gaccccgaga
      301 ttgttccccg gctcagagaa gcgaccatgg tgatcgaaac ccacgaacag ttccgcccga
      361 acgtaatcga aaagttgaac gaacggttct ctgacaccca tgacatctcc aggatcttca
      421 aagaagtacc cgggcgaaag tcctgcccgg aagtgaagga ctggagtgac ggagatttcg
      481 agattgcagc atacgacggg catcattccg gtgacagttg gatgactctc gtgccgaaga
      541 agcctggatg atcccgcagg atgactgccc gcccacgctt cggtcgcatg atcggacttg
      601 catcgagtta gggttagggc atgaaccggc gtgaacagat cgagatgacc caagaggaag
      661 ccgaggcttt tctacttgag cgcaaagtgg ctgtcgtgac cagtttcggc cctcggggct
      721 ggcctcattc gatgccgctc tggtacgtcc cgaacgggga ccggatcagg tcctggactt
      781 tcgccaagtc ccagaaggtc aagaacctcg aacgtgatcc gcgcgcgacc gtgctagtcg
      841 aggatggcta cgacaattat tccgagctca ggggcgtgat gatggagacc gagacgctca
      901 tacatcgtga tcccaacctg gttaccgcat acgcggagag ttcggttgcc cgatatacgc
      961 ctgaaggggt cgagctcacc accgaaatgc tcgaggcgtt tcgtagtcaa gcgccaaaga
     1021 gggtggtcct tgagttcatt ccagtcagag ttctgacctg ggatcatcgg aaactgggag
     1081 gaacctactg accggtggat cggttgcgag attgggcaag tgagccacag tggagctcag
     1141 taacgtcggc gattgagccg cacccgcatg tgacgacgca agctgggcgg ttcgagcagc
     1201 tcaaaggggg ggtcgattgg aacgatgtcg cgccccgctt gccagccttc aattcggccg
     1261 ttaaggccag ctgcagtccg gtcgtgaagc agttgggcga ggctggccga gccatcgctg
     1321 atgagggtca tgatcgccag tcctggatga gccatgatcc cgtatttcct caacaggtag
     1381 ccgcggctga agccggtccg tcggtacttg gcggcagccc tccggcctag ggtcgcggaa
     1441 ctctcgtggg tcgccctcgc cgctcttgcc agacggcagg aatagcctgc catacgcatt
     1501 cgcagggcaa ggtcgagatc ctcgtagtag aggaagattc gctcatcaaa ccccccaatc
     1561 gtttcgaaag cgacccggtc gaagagggcg gcgccgccgg tcgggcccaa ggggtccgac
     1621 gcgacttcca agcgcgttgc cggttctcca gtcagatgat cccaggcagt gagggtcctg
     1681 tcgcagcgga ttccggcaga gtccaca
//
LOCUS       DAOQFI010000506         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46699_length_1705_cov_3.240000, whole genome shotgun sequence.
ACCESSION   DAOQFI010000506 DAOQFI010000000
VERSION     DAOQFI010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46699_length_1705_cov_3.240000"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..>1705)
                     /locus_tag="PLE93_08650"
     CDS             complement(67..>1705)
                     /locus_tag="PLE93_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87159.1"
                     /translation="IGYLGFVLRFVRFFLLALLISTVVSVPARAGTIVADSGFRPGTD
                     GFSFANYGADEGYRNLDAFEVQKIYGRAACLTGKGATCVLNPGVRNWMRSTNEAMAGG
                     HCYGFSALTQLIYKNELPRFGYSSISAFGGSSPFGLNIVGNVRLQRSIARAFTYQLLP
                     SVNAQATMGTPKHVLRYLIDHLGDGSQQSWNLLIFQWGFQAGHAITPYAIEDMGGGIY
                     EIHVYDNNWPNDDTRRLVVNTNRNTWSYYASTQPGIPAAEYRGNARSGTLFLRPNTPA
                     LGIQPCPYCIGRQGANSKYNQVTLSYTADQHARLLITDSKGRQTGFKDGKPINRIPGA
                     KVIRQATSPITFAADGAIENIADDPEPVYLIPKNLKLRIRIDGRHMTVTDRESLGVVG
                     PTFDSTVENLKMGPGKVAFATLSPKAKTLSITGARGESSPRVTFGAQSRKAAYRVKVS
                     AIGAAPQSTFYFAKKPNYGLLRIGKKATGPQAWKVAINKFDARGNQTRFVRSYVLRGN
                     QIAFLYYGPLAVGKRAYVVIASPNGNKVKLLKLKRSQ"
BASE COUNT          283 a          508 c          593 g          321 t
ORIGIN      
        1 tggcccgcgc tcccatctca acccgcccct cccactcgaa gaaaaggatg gttgggaggg
       61 gctctgttat tggctcctct tgagcttcag gagcttgact ttgttgccgt tgggggaggc
      121 gatcacgacg taggcgcgct tgccgactgc gagcggcccg tagtagagga aggcgatctg
      181 gttgccgcgc aggacgtagc tgcggacgaa ccgggtctgg ttgccacggg cgtcgaactt
      241 gttgatcgcc accttccagg cctggggacc ggtcgccttc ttgccgatcc tgagcagacc
      301 gtaattgggc ttcttggcga agtagaaagt cgattgcggc gccgcgccga tcgcactcac
      361 cttgacccgg taggcggcct tcctgctctg ggccccgaag gtgacccgcg gcgaggattc
      421 acccctggcc ccggtgatcg aaagggtctt ggcctttggc gaaagggtcg cgaaggcgac
      481 cttgcccgga cccatcttca ggttctcgac ggtcgagtcg aaggtcgggc cgaccacccc
      541 cagcgattcg cggtcggtga ccgtcatgtg cctgccgtcg atccggatcc ggagcttcag
      601 gttcttgggg atgaggtaga ccggttcggg atcgtcggcg atgttttcga tcgcgccgtc
      661 ggccgcgaag gtgatcggcg aggtcgcctg ccggatgacc ttggccccgg ggatccggtt
      721 gatcggcttg ccgtccttga acccggtctg gcgccccttc gaatcggtga tcagcagccg
      781 tgcgtgctga tcggccgtgt aggagagggt gacctggttg tacttcgagt tggctccctg
      841 gcggccgatg cagtacgggc agggctggat cccgagggcg ggcgtgttcg ggcggaggaa
      901 gagggtccct gagcgggcgt tgcccctgta ctcggcggcg gggatgccgg gctgggtgga
      961 cgcgtagtag ctccatgtgt tccggttcgt gttgacgacc agcctgcggg tgtcgtcatt
     1021 cggccagttg ttgtcataga cgtgaatctc gtagatgccg ccccccatgt cctcgatcgc
     1081 gtatggggtg atcgcgtgac cggcctggaa gccccactgg aagatcagca ggttccagga
     1141 ctgctgagag ccgtccccga ggtggtcgat caggtaccgg agcacgtgct tcggagtgcc
     1201 catcgtcgcc tgcgcgttga ccgagggaag cagctggtag gtgaaggccc gggcgattga
     1261 ccgctggaga cggacgttgc cgacgatgtt caggccgaag ggggagctgc cgccgaaggc
     1321 ggagatcgag ctgtaaccga agcgcgggag ctcgttcttg tagatcagct gggtgagcgc
     1381 ggagaacccg taacagtgcc cgccggccat cgcctcgttg gtcgacctca tccagttgcg
     1441 gacgccgggg ttcagaacgc aggtcgcgcc cttcccggtc aggcaggccg cccggccgta
     1501 gatcttctgc acctcaaagg cgtccaggtt cctgtaaccc tcgtcggcgc cgtagttggc
     1561 gaacgaaaac ccgtccgttc cgggccggaa gccggagtcc gcaacgatcg tccccgcccg
     1621 ggcaggcacg gaaaccacgg tcgagatgag cagggccagc aggaaaaacc gaacaaaacg
     1681 aagcacaaac ccaaggtacc caatc
//
LOCUS       DAOQFI010000507         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46709_length_1705_cov_2.943636, whole genome shotgun sequence.
ACCESSION   DAOQFI010000507 DAOQFI010000000
VERSION     DAOQFI010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46709_length_1705_cov_2.943636"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PLE93_08655"
     CDS             <1..940
                     /locus_tag="PLE93_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNH87160.1"
                     /translation="ETWELTAERTTREANLFEKWTPFAEGLLPEIFDFDADNHVLAME
                     DLSGFAILADAFGADDQTANVERVGRLLARAFLNTSPFFLDPAEFSARLVEAENPELA
                     ELMEQVVFDQPWESEPLDHCHADLHEEFGEMLADPSLQARIGELKFIFRTVHEGLIHG
                     DLHTGSVMVAGDDLRVFDAEFARYGPIAWDVGEFIGHLRVAAVALEFEGDLAGAKACD
                     LLTARFWDAFEEEVRDGWSRRTDRTFPDPFLDRWLVGQYELAIRFAGAEIARRITGVG
                     KANQIDALPEARMLDASRKLLGEARQLISSGRPAWI"
     gene            931..1689
                     /locus_tag="PLE93_08660"
     CDS             931..1689
                     /locus_tag="PLE93_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNH87161.1"
                     /translation="MDLELEAAAATEAAVMVGPMLRRCFENGVVETRSKAVRQDVVTQ
                     ADLDAEQAIIGHLRSKWPEDGFLGEESGSSDGTSARVWCVDPLDGTANFAAGYPRWAV
                     AIALLIRGEAVLAVTFDPLREEIFVAREDVGLTSGPGLSNMRAVARIEDALVADNRPY
                     DHREPAQPLEMEFRRSRGRRESGSMALDFAWLAAGRLDVVSYERSERLWDYIGGELLV
                     AQAGGEIRIADPARDIAVAASPELVEYLCPTKDE"
BASE COUNT          296 a          534 c          573 g          302 t
ORIGIN      
        1 cgagacttgg gagctgaccg ccgaacggac cacccgcgaa gcgaacctgt tcgaaaagtg
       61 gactcccttc gccgagggac tcctgccgga gatcttcgat ttcgacgcgg acaaccacgt
      121 gctggcgatg gaggaccttt cggggttcgc catcctggcc gatgccttcg gtgccgacga
      181 ccagaccgca aacgtcgaac gggtgggtcg cctgctcgcc agggcctttc tcaacacttc
      241 gcccttcttt ctcgacccgg cggagttctc tgcccgtctc gtcgaggccg aaaatcccga
      301 actggccgag ctgatggaac aagtcgtgtt cgaccagccc tgggaaagcg agccgttgga
      361 tcactgccac gcggacctgc acgaagaatt cggggagatg ctggccgacc cgtcgcttca
      421 ggccaggatc ggggaactca agttcatctt ccggacggtc cacgaaggac tcattcacgg
      481 cgatctccac accggcagcg tcatggtggc cggggacgac cttcgagtct tcgacgccga
      541 gttcgcccgg tacggaccga ttgcgtggga cgtgggggag ttcatcgggc acctgagggt
      601 cgctgccgtt gccctcgagt tcgagggtga ccttgccggc gcaaaggcct gcgatttgct
      661 cacggcccgc ttctgggacg cgtttgagga agaggtgcgc gacggttgga gccggcggac
      721 cgaccggacg ttccccgacc ccttcctcga tcgctggctc gtcggccagt acgaattggc
      781 cattcgcttc gccggggccg agatcgcccg aaggatcacc ggggtcggca aggcgaatca
      841 gatcgatgcc ctgcctgagg cccggatgct cgatgccagc cgcaagctgc tcggcgaagc
      901 gcgccagttg atctcgtccg ggaggcccgc atggatctag aactggaagc tgccgcggcg
      961 accgaagcgg ccgtcatggt cggtccgatg cttcgccggt gtttcgagaa cggggtcgtg
     1021 gaaacccgga gcaaagcagt gcgtcaggac gtggtaaccc aggcggacct cgacgccgaa
     1081 caggcgatca tcggccacct gaggagcaag tggccagagg atggcttcct gggcgaagag
     1141 agcggaagct cagacggcac atcggcccgg gtgtggtgcg tcgatccact cgacggaacc
     1201 gccaacttcg ctgccggcta tccccgttgg gccgttgcga tcgcgctctt gattcgaggc
     1261 gaggcagtgc tggccgtcac gttcgatcct ctgcgagagg agatcttcgt cgcccgggaa
     1321 gatgtcggtc tgacttcagg cccgggtctt tcgaatatgc gtgcggtcgc ccgaatcgag
     1381 gatgccctgg tcgcggacaa ccggccgtac gatcaccgcg agccggccca gcccctggag
     1441 atggagttcc gtcggtcgcg gggccggcgc gagtccggtt cgatggctct cgactttgcc
     1501 tggctcgcgg caggccggct tgacgtggtc agttacgagc gctcggagcg gctctgggac
     1561 tacatcggcg gcgagctcct cgtagcccag gccggaggcg aaatacgaat cgccgacccg
     1621 gctcgtgaca tcgccgtcgc tgccagcccc gaacttgtcg aatacctgtg cccgacgaag
     1681 gatgagtaga tgactggaca gcctg
//
LOCUS       DAOQFI010000508         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46714_length_1705_cov_2.653939, whole genome shotgun sequence.
ACCESSION   DAOQFI010000508 DAOQFI010000000
VERSION     DAOQFI010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46714_length_1705_cov_2.653939"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLE93_08665"
     CDS             <1..577
                     /locus_tag="PLE93_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH87162.1"
                     /translation="EYGPAAFKLDLAVEDGIPWESEAARKAGTVHVIGSFEELVISEH
                     ETHAGRMPERPFILVGQQYLADPSRSNGNLHPVWAYAHVPNGFTGDATEAMVSQIERF
                     APGFRDRIRGETAMYPEDFAQYNANYVGGDIVAGANTPRQMVFRPRVTLNPYKVTEGI
                     WICSAATPPGGGVHGMGGANAARSILRTLGS"
     gene            complement(643..>1705)
                     /locus_tag="PLE93_08670"
     CDS             complement(643..>1705)
                     /locus_tag="PLE93_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87163.1"
                     /translation="GWLEEFTGGKALQGAKYIRDTRIAGGVATETAEVATSASRLTGA
                     GAKALPDAPSLGNLSNKQIDRFKQIVKNVNEKFGVKVEIQARPINEYAAKVKGGIGKV
                     EAVPTKNLTPDDVILGAPPEWVGQAGYYPPKLPKNFNKLDAAEKARLQLRFDEKTKEF
                     KQFKGVIEDPTGKTAKVHKALKKETEFDLGKNGKLKMELEATTHKSGAVLISYKKLSV
                     NGKPVFTGKPRPIISDVDMNAVVDAATGRHLPAGIRGQVELEVMRQFAKAAEEGVFPF
                     GYHGWTHSGFDIAAADFRHILKYQLMYMNEKDAVKLAKKYAAIYGIDPSEFLDGYTRG
                     KLLVKITATDAVLGPGAGL"
BASE COUNT          272 a          540 c          551 g          342 t
ORIGIN      
        1 cgagtacggg ccagccgcct tcaagctcga cctcgcggtc gaggacggga tcccctggga
       61 aagcgaagcc gcgcggaagg ccggcaccgt ccacgtgatc ggttccttcg aggaactcgt
      121 gatctccgag cacgagaccc acgccggccg gatgccggaa aggcccttca tcctggtcgg
      181 ccagcagtac ctcgccgacc cctcaaggtc gaacggaaac ctccacccgg tctgggccta
      241 cgcgcatgtg ccaaacgggt tcaccggtga cgcgaccgag gcgatggtca gccagatcga
      301 acgcttcgca cccggtttcc gcgaccggat ccgcggggag acagccatgt accccgagga
      361 tttcgcccag tacaacgcca attacgtggg cggcgacatc gtcgccgggg cgaacacccc
      421 gaggcagatg gtcttccggc cccgggtcac gctgaacccg tacaaggtca ccgagggaat
      481 ctggatctgc tccgccgcga ccccgcccgg cggcggcgtc cacggcatgg ggggcgccaa
      541 cgcggcacgc tcgatcctcc gcacgctcgg tagctgagaa gcatctgggt tgaagttatt
      601 cgttccatgt gcggataact tcaacgtaga tgcaggttca gcctagaggc cggcgcccgg
      661 gccgaggacg gcgtcggtgg cggtgatctt gaccaggagt ttgccgcggg tgtagccgtc
      721 gaggaactcg gaagggtcga tgccgtagat cgcggcgtac ttcttcgcga gcttgaccgc
      781 gtctttctcg ttcatgtaca tgagctggta cttgaggatg tgacggaagt cggcggcggc
      841 gatgtcgaat ccggagtggg tccagccgtg gtaaccgaag gggaagacac cttcctcggc
      901 ggccttggcg aattggcgca tcacctccag ctcgacctgg ccgcgaatgc cggcgggcag
      961 gtgacggccg gtggccgcat caaccactgc gttcatgtcc acgtcggaga tgatcggcct
     1021 gggcttgccg gtgaagaccg gcttgccgtt gaccgagagc ttcttgtagc tgatcagcac
     1081 cgccccggac ttgtgggtgg tcgcttcgag ttccatcttc agcttgccgt tcttgcccag
     1141 atcgaactcg gtttcctttt tcagggcctt gtgaaccttt gccgtcttgc ccgtgggatc
     1201 ctcgatgacg cccttgaact gcttgaactc cttggtcttt tcgtcaaagc gaagctggag
     1261 gcgggctttc tcggccgcgt cgagtttgtt gaagttcttc gggagcttcg gcgggtagta
     1321 accggcctgg ccgacccatt cgggcggagc cccgaggatc acgtcgtccg gggtcagatt
     1381 cttggtcggg accgcctcga ccttgccgat tccgcccttg accttcgccg cgtactcgtt
     1441 gatcggccgg gcctggattt cgaccttgac cccgaacttt tcgttgacgt tcttgacgat
     1501 ttgcttgaac cggtcaatct gcttgttgct caggttgccg agtgaaggcg cgtccgggag
     1561 ggccttggca cccgcgccgg tgagacggct ggcgctggtc gcgacctcgg cggtttccgt
     1621 ggcgacaccg ccggcgatcc gcgtatcccg aatgtacttc gccccctgca gggccttgcc
     1681 gcccgtgaac tcctcgagcc agccg
//
LOCUS       DAOQFI010000509         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46860_length_1702_cov_3.442016, whole genome shotgun sequence.
ACCESSION   DAOQFI010000509 DAOQFI010000000
VERSION     DAOQFI010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46860_length_1702_cov_3.442016"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PLE93_08675"
     CDS             complement(<1..997)
                     /locus_tag="PLE93_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNH87164.1"
                     /translation="MSRSERILITGAREHNLKGIDLDLPRDALIVVTGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGLMEKPDVDSIEGLSPAISIDQKTTSRNPRSTVG
                     TVTEIYDYLRLLWARIGHPHCPNCGKPITGQTQEQITDQLMTMEEGKRFMVMAPVVRG
                     RKGEYGKLFDQFRADGYSRVEVDGEMRMLDQEIVLDKKYKHDISVVVDRLVMKPDLRR
                     RLSESVEAAASLAEGLVEIEVIDGERQTFSESFACLECGTSMPELEPRIFSFNAPHGA
                     CPACTGLGFKKVIDPELVVPDPTLSVAEGALQPWRSGYSGYWRRLNKSVAETYGID"
     gene            complement(1070..1543)
                     /locus_tag="PLE93_08680"
     CDS             complement(1070..1543)
                     /locus_tag="PLE93_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87165.1"
                     /translation="MEREHLVRTDFPRAHGSEGFDIDSVGAHLAAVAAHVAALEARIT
                     ALQVECDTLRTQLADARDHEDGPSGLPDPAGPDPLQKLGAEVEQAPVETMPADPEPDP
                     EAGAAPGEEEAAARMLASKLAMGGTSRDTIVMQVAAQHEIDSPGALVDDVLARLA"
BASE COUNT          274 a          567 c          556 g          305 t
ORIGIN      
        1 tgtcaatgcc gtaggtctcg gcgaccgact tgttgaggcg gcgccagtat ccggagtagc
       61 cggaacgcca gggctgcagc gcgccttcgg ccaccgacag ggtcgggtcg ggcacgacca
      121 gttccgggtc gatcaccttc ttgaagccga gcccggtgca ggccggacag gcgccgtggg
      181 gcgcgttgaa ggagaagatg cgcggctcca gttccggcat cgaagtgccg cattcgaggc
      241 aggcaaaact ctccgagaag gtctggcgct cgccgtcgat cacctcgatc tctaccaggc
      301 cttcggccag tgacgccgct gcttcgaccg actcggagag gcggcggcga aggtccggct
      361 tcatgaccag ccggtcgacg accaccgaga tgtcgtgttt gtacttcttg tcgaggacga
      421 tctcctggtc cagcatccgc atctcgccgt cgacctcgac ccgcgagtat ccgtcggcgc
      481 ggaattgatc gaagagcttg ccgtactcgc ccttcctgcc gcgaaccacc ggcgccatga
      541 ccatgaaccg cttgccctcc tccatggtca tcagctggtc ggtgatctgc tcctgggtct
      601 gcccggtgat cggcttgccg cagttcgggc aatgcgggtg accgatccgg gcccagagga
      661 ggcgcaggta gtcgtagatc tcggtcaccg tgccgaccgt cgaacgggga ttccgcgagg
      721 tcgtcttctg gtcaatcgag atcgccggag aaagcccttc gatcgaatcg acatcgggct
      781 tttccatcag tccgaggaac tgcctggcgt aggcggaaag cgactcgaca tagcggcgct
      841 ggccctccgc ataaatcgtg tcgaaggcaa ggctggactt gccggaaccc gacagtccgg
      901 tcaccacgat cagggcatcg cgaggcaggt cgagatcgat gcctttgagg ttgtgctcac
      961 gggcgccggt aatcaggatg cgttcagaac ggctcacgcc ccccaatatt agggaaccga
     1021 acacacgttc gtttgcaccc gaaccccttc catgccgcgc agggcgggtt caggcgagtc
     1081 gggcgagcac gtcgtcgacg agagcgccgg gactgtcgat ctcgtgctgc gcggcgacct
     1141 gcatcacgat cgtgtcgcgg ctggtaccgc ccatcgcgag tttcgaggcg agcatccggg
     1201 ccgccgcctc ttcctcgccg ggtgcggctc ccgcctcggg atccggttcg gggtcggccg
     1261 gcatcgtctc gacgggcgcc tgctcgacct cggcacctag cttctgcagc gggtcaggcc
     1321 cggccggatc gggaaggccg ctcggaccgt cctcgtgatc gcgcgcatcg gcaagctggg
     1381 ttcgcagggt gtcgcattcc acctgcaggg cggtgatccg ggcttccagc gcggcaacgt
     1441 gcgccgccac cgcggccagg tgggcgccga ccgagtcgat gtcgaaacct tcggacccgt
     1501 gggcccgcgg aaaatccgtt cgaaccaggt gctctcgctc cattcagaaa gcctagcgct
     1561 ccccggaccg cggccgttcc ggctcaggcc ccggccttca tcgcgtcgag atcccggcgc
     1621 agttcccgga tctcgtcacg cagacgggcg gcctgctcga agcggagatc ctcggcggcg
     1681 gcatgcattt cctcttccat ct
//
LOCUS       DAOQFI010000510         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46872_length_1702_cov_3.104432, whole genome shotgun sequence.
ACCESSION   DAOQFI010000510 DAOQFI010000000
VERSION     DAOQFI010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46872_length_1702_cov_3.104432"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..546)
                     /locus_tag="PLE93_08685"
     CDS             complement(265..546)
                     /locus_tag="PLE93_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87166.1"
                     /translation="MGYEYVFIALLFGIATGIIGRAKGGSFLIWLVVGTILPGLGLIA
                     VFMMRNEVNEPERRCENCGKVLKLYVQVCPRCGYDLYLPPPEETRMPPR"
     gene            696..>1702
                     /locus_tag="PLE93_08690"
     CDS             696..>1702
                     /locus_tag="PLE93_08690"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNH87167.1"
                     /translation="MAVKAGKSTRFRSYRRQRWVDSGEGVVRLKTKVIRRRRGEKAPR
                     KDPGPRPLPRPQIPRNPWKPQPFGTYHGEFGRLEATRLLNRAGFGPRPGQAEQLAKMG
                     MKKAVLSLTRPQGQAKLIGPEPVDGDGLPLAPFDSWGHDHLWWLDRMVRTDQPFRERM
                     ALVFHDWFATSNSGVSQQRQMIDQYWTLRAGVNGTFDDLVVQITKDPAMLQWLSGGDN
                     TKWSPNENYARELMELFTLGADRGAYTEDDIREAARALTGWTYDWDDDLGAINFRFDP
                     EYHDDETKKIFSSKGNWDWRDVCRLCVTHELHASFFVDKLWSYFVPAAPSKDTRQALI
                     RL"
BASE COUNT          320 a          547 c          558 g          277 t
ORIGIN      
        1 aacgccatag tccgaacctg gcttcgctga cttaactgca agtcgaacgg ggtccccgac
       61 gccacggctg ccaggacccg gtcgagcccc gaaggtgccg caggtttcag accggatcgg
      121 gcccaaacct gcggcacctc aatcgtcgac ccgggtccaa cccggtcagc ggacggcgag
      181 atcccatttt gcgccacata ccggcacaaa gtgggcactc gatgattcag ccccacgcca
      241 acgctgcggg ccgtttgccg cgccttaccg ggggggcatt cgggtttctt ctggtggtgg
      301 caggtagagg tcgtagccgc atcttgggca gacctggacg tagagcttga ggaccttgcc
      361 gcagttctcg cagcggcgtt ccggttcgtt gacttcgttc cgcatcatga agacggcgat
      421 caggccgagc ccgggcagga tcgtgccgac gaccagccag atcaggaagg agccgccctt
      481 ggcgcggccg atgatcccgg tcgcgattcc gaagagcagg gcgatgaaga cgtattcgta
      541 acccacgccg acattgtgcc ggggtaccgc tccgcttgac ggccgatcgg gttgtggatg
      601 ggacgagacc gttcggttct ggtctggaaa cgggcgccgg cgatggggcg aagaaggttc
      661 cgcttgacgg ttggtgaaca atgtttgggc aggggatggc tgtaaaggca ggaaaaagca
      721 cgagattcag gagctacagg cgccagcgct gggtcgactc cggcgagggc gtcgtccgcc
      781 tgaagacgaa ggtgatccgt cgccgcaggg gcgagaaggc tccccgcaag gatcccggcc
      841 cccggccgct gccccggccg cagattcccc gcaacccctg gaagccgcag ccgttcggca
      901 cctaccacgg agagttcggt cggctcgaag ccacccgcct gctgaaccgg gcgggcttcg
      961 gcccgcggcc aggtcaggcg gagcagctgg cgaagatggg aatgaagaag gcggtccttt
     1021 ccctgacccg gcctcagggc caggcgaagc tgatcgggcc cgaaccggtc gacggcgacg
     1081 gcctgccgct tgcgccattc gattcctggg gtcacgatca cctctggtgg ctcgaccgca
     1141 tggtccggac cgatcagccg ttccgggaac ggatggccct ggtcttccac gactggttcg
     1201 caacctcgaa ctccggggtc tcccagcaac gccagatgat cgaccagtac tggaccctgc
     1261 gggccggcgt gaacgggacc ttcgatgacc tggttgttca gatcaccaag gatccggcga
     1321 tgctccagtg gctgagtggc ggcgacaaca ccaagtggtc accgaacgag aactacgccc
     1381 gagaactgat ggaactcttc accctcggtg ccgaccgggg cgcctacacc gaggacgaca
     1441 tccgcgaggc ggcccgggcc ctgaccggct ggacctacga ctgggacgat gacctcggcg
     1501 cgatcaattt ccgcttcgac cccgagtatc acgacgacga gaccaagaag atcttcagca
     1561 gcaaggggaa ctgggactgg cgggacgtct gccggctctg cgtcacccat gaactccacg
     1621 ccagtttctt cgtcgacaag ctctggagct acttcgttcc ggccgcgccc tcaaaggaca
     1681 cgcgacaggc cctgatccgg ct
//
LOCUS       DAOQFI010000511         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46915_length_1701_cov_3.667072, whole genome shotgun sequence.
ACCESSION   DAOQFI010000511 DAOQFI010000000
VERSION     DAOQFI010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46915_length_1701_cov_3.667072"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLE93_08695"
     CDS             <1..601
                     /locus_tag="PLE93_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87168.1"
                     /translation="SWTERDGQPVVRSTRPGHLEPGNVAVVERDGRRLDFFPLSSGAN
                     GDGQGEVPQKQPRDGAKRAAPSPGEGRETVTDRRGRQWSISGNIEALGASVEEGRIET
                     PEYPDGLGRLWSALVAPHGGDIIVSAAPGYECVDWGGAAHVPGGSHGSLRKEDSEGPL
                     LFVGCGPDSPDPETAARHRSRPQWGLQDVAPIIREFFGT"
     gene            complement(645..1445)
                     /locus_tag="PLE93_08700"
     CDS             complement(645..1445)
                     /locus_tag="PLE93_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNH87169.1"
                     /translation="MKLLFIGDVVGSPGRKGLASLMPRLREEHSPDLIVVNGENSAGG
                     LGITEKTAKEIFACGADVITLGNHTYRQRESWDYLDRAERVIRPANYRRANPGKGHCV
                     VQVNPADGSPARRVAVINLIGKIGLDAARSPFDEADLILNDLEKSGAADTVVVDFHAE
                     VTSEKVAMGWYLDGRVAAVFGTHTHVPTADGRVLPGGTAFISDVGMTGARDSVLGVKK
                     EQAIEKMRTDMPIRFESATDDVWVMGAAVEIDENGLATGFKQILEPSS"
     gene            1481..>1701
                     /locus_tag="PLE93_08705"
     CDS             1481..>1701
                     /locus_tag="PLE93_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH87170.1"
                     /translation="MQTPLTGTGPALAARGVSKSFDGRKALRGVDFEVGTGEVVALIG
                     PNGAGKTTLLSILAGITEPDSGEVTGNGVA"
BASE COUNT          269 a          580 c          582 g          270 t
ORIGIN      
        1 cagctggacc gaaagggacg gccagccggt cgttcgctcg acccgccccg gccacctcga
       61 accgggcaac gtcgcggtcg tggaaaggga cggtcgccgg ctcgacttct tccccctgag
      121 ttcgggggca aatggcgacg ggcagggaga ggtgccgcaa aaacagcccc gtgacggggc
      181 aaaacgtgcg gcaccttccc cgggtgaggg ccgtgaaacg gtgaccgacc ggcgcgggcg
      241 acagtggtcg atcagcggca acatcgaggc tctcggggcc tcggtcgagg agggccggat
      301 cgagacgccc gaatacccgg acgggctcgg ccggctctgg tcagccctcg tcgcccccca
      361 tggcggagac atcatcgtct ccgccgcgcc cggctacgaa tgcgtcgact ggggcggagc
      421 cgcccacgtg cccggcggca gccacggttc gctccgcaag gaggattccg aaggcccgct
      481 gctcttcgtc ggctgcggcc ccgactcacc ggacccggaa accgccgccc gccaccggtc
      541 ccgcccccaa tggggcctcc aggatgtggc cccgataatc agggaattct tcggcacctg
      601 agaggagccg ggtccggccc cctcccggag acatccgccc tccgctagga cgagggttcg
      661 aggatttgct tgaacccggt tgcgaggccg ttttcgtcga tctcgaccgc ggcgcccatt
      721 acccagacat catcggtggc ggattcgaag cggatcggca tgtcggttcg catcttctcg
      781 atcgcctgtt ccttcttcac tccgaggacc gaatcgcggg cgccggtcat gccgacgtcc
      841 gagatgaagg ccgtgcctcc gggaagcacc cgcccgtcgg cggtcggcac gtgggtgtgg
      901 gtgccgaaca cggcggcgac ccggccgtcc agataccagc ccatcgccac cttttcgctg
      961 gtcacctcgg cgtggaagtc aacgaccacc gtgtcggcgg cgccgctctt ctcgaggtcg
     1021 ttcaggatca ggtcggcctc gtcgaaaggt gaccgggcgg cgtcgaggcc gatcttgccg
     1081 atcaggttga tcaccgcgac ccgtcgggcc ggcgagccgt cggccgggtt cacctggacc
     1141 acgcagtggc ccttgccggg attggcccgc cggtagttgg cggggcggat cacccgctcg
     1201 gcccggtcca ggtaatccca gctttcgcgc tggcggtagg tgtgattgcc aagcgtgatc
     1261 acgtcggcgc cgcaggcgaa gatctccttt gcggtcttct cggtgattcc gaggccgccg
     1321 gccgagttct cgccgttgac cacgatcagg tccggcgaat gttcttcgcg cagtcgcggc
     1381 atcagcgaag ccagcccctt gcggccggga ctcccgacga catcaccgat gaagagcagc
     1441 ttcacgcttg cgagcctaaa ccttgggtag gttcaacctc gtgcagacac cgctcacagg
     1501 cacaggcccc gccctcgcgg cgcggggggt cagcaagtcc ttcgatggac ggaaggcgct
     1561 caggggagtc gacttcgagg tcgggaccgg cgaggtcgtg gcgctgatcg ggccgaacgg
     1621 cgccggcaag acgacgctgc tgtcgatcct ggccggcatc accgaacctg actcgggcga
     1681 ggtgacggga aacggtgtcg c
//
LOCUS       DAOQFI010000512         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_46916_length_1701_cov_3.665857, whole genome shotgun sequence.
ACCESSION   DAOQFI010000512 DAOQFI010000000
VERSION     DAOQFI010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46916_length_1701_cov_3.665857"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLE93_08710"
     CDS             <1..186
                     /locus_tag="PLE93_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87171.1"
                     /translation="KTDELTLDSASRQAIEDAGEESVQEALVELALRTGARVSSASVD
                     EVPALTEAGGVLALLRY"
     gene            257..330
                     /locus_tag="PLE93_08715"
     tRNA            257..330
                     /locus_tag="PLE93_08715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:291..293,aa:Arg,seq:cct)
     gene            391..795
                     /locus_tag="PLE93_08720"
     CDS             391..795
                     /locus_tag="PLE93_08720"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNH87172.1"
                     /translation="MAKMKRKPISTRKPPVPADDSTDVDDWTSRVMPDLQPIVASLDQ
                     QIRKAIPDLQYAVKWKKAYYGREDLGWVIEMVAYDISVNMVFLGGADFDSPPPLGDTD
                     RSRYIKIKSLEEAETPEMGAWIEQAGRVPGWK"
     gene            817..1218
                     /locus_tag="PLE93_08725"
     CDS             817..1218
                     /locus_tag="PLE93_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH87173.1"
                     /translation="MPFTRINLKQDVDNFGPNFDGSPDLDFRAATKPLELEHSFLGYQ
                     RIPPGYRFPFGHTHDSQEEVYVVVGGSGRMKLDDEIIELGKWDAVRVPPGTWRGYEAG
                     PDGLEILISGAPNLGDQVRDDVTGQRDWWQD"
     gene            1226..>1701
                     /locus_tag="PLE93_08730"
     CDS             1226..>1701
                     /locus_tag="PLE93_08730"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNH87174.1"
                     /translation="MADWADDFFVEWQRAWGTGTEAVLEFVTDDIEYWDVTLPEPIRG
                     REAYARHCDNFFTAFTECEWSLREPVICEGDRVTEPWAFTGLNSGPIWIGLPATGRRL
                     ESKGTDIFEFRDGKVCREHSFYDVVGNLRALGLWPGIGSRTEAAAMTAAGAAIRGTG"
BASE COUNT          311 a          537 c          573 g          280 t
ORIGIN      
        1 aagaccgacg agctgaccct cgattcggcc agccggcagg cgatcgagga tgcgggcgaa
       61 gaaagcgtcc aggaggctct ggttgaactc gccctccgga ccggagcccg ggtcagctcg
      121 gcctcggtcg acgaggtccc ggcattgacc gaagccggcg gcgtgctggc cttgcttcgc
      181 tattgagcgg cgcccaaggc catcccggcc ctccgccggg accgaatcag gcctccacgg
      241 atagcatccc ccttcggccc cggtagctca gctggataga gcgtccgcct cctaagcgga
      301 aggccgctgg ttcgaatcca gctcggggcg tgatcgctcg ggtgccacgc cgggcgaggc
      361 aggtgaggca accaaccagg aggattcgaa atggcaaaga tgaagcgcaa gccgatctcg
      421 acccgcaagc cgccggtgcc ggctgatgac tccactgacg tcgacgactg gacctcgcgg
      481 gtgatgccgg acctgcagcc gatcgtcgct tcactggatc agcagatccg caaggcgatc
      541 cccgacctgc agtacgcggt gaagtggaag aaggcctact acggtcggga agatctcggc
      601 tgggtcatcg agatggtcgc ctatgacatc tcggtcaaca tggtctttct cggcggtgcc
      661 gacttcgatt cgccgccgcc gctcggggac acggaccgca gccgctacat caagatcaag
      721 tcgctggaag aagccgagac accggagatg ggagcctgga tcgaacaggc cggccgcgtc
      781 ccgggctgga agtagccggg gccgtaggat cgccccatgc cgttcacgcg catcaacctc
      841 aagcaggacg tggacaactt cgggcccaac ttcgacggct cgccggacct cgatttccgg
      901 gccgcgacga agccgctcga actcgagcat tcattcctgg gttatcagcg tattccgccg
      961 ggatacagat ttcccttcgg ccacacccac gacagccagg aagaggtcta cgtggtcgtc
     1021 ggcggcagcg gcaggatgaa gctcgatgac gagatcatcg aacttgggaa atgggatgcg
     1081 gtgagggtgc cacctggcac ctggcgaggc tatgaggccg gaccggatgg cctcgagatc
     1141 ctgatttccg gcgctccgaa cctcggcgat caggttcgtg acgacgtgac cggccagcgc
     1201 gactggtggc aggactgaga tcgccttggc tgactgggcg gatgacttct tcgttgagtg
     1261 gcagcgggcc tgggggaccg ggaccgaggc ggtgctcgag ttcgtcaccg atgacatcga
     1321 gtactgggat gtgaccctgc ccgagccgat tcggggccgt gaggcctatg cgaggcattg
     1381 cgacaacttc ttcaccgcct tcactgaatg cgagtggtca ctgcgcgaac cggtgatctg
     1441 cgagggcgac cgggtgaccg agccctgggc cttcaccggg ctcaattcgg gtccgatctg
     1501 gatcggcctg cctgccaccg gccgccggct ggaatcgaag ggcaccgaca tcttcgaatt
     1561 ccgggacggc aaggtttgcc gcgagcattc cttctacgac gtggtcggca atctccgggc
     1621 cctcggcctc tggcccggca tcggcagccg gaccgaagcc gcggcaatga ccgcggcggg
     1681 ggctgcgatc cgcggcaccg g
//
LOCUS       DAOQFI010000513         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47082_length_1698_cov_4.486914, whole genome shotgun sequence.
ACCESSION   DAOQFI010000513 DAOQFI010000000
VERSION     DAOQFI010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47082_length_1698_cov_4.486914"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="PLE93_08735"
     CDS             complement(<1..1622)
                     /locus_tag="PLE93_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87175.1"
                     /translation="MPDGKRLVTVVASPDLTAPQLAITRLDGSHPKCVTCGLDTAVVP
                     GAPVDAAFGKPAPFPDGKRLMVRLGLRPGAGGSPTQNFTYGVVECTPTLVNCEERTLS
                     PIIMPGGGIDQGTQNREGRLSPDGRWFGWTEFTMNGTAMSVGRLAKNGGQYDLENVRI
                     LNPSGPLGTDSAAWRAQSPYFELKNFTSDGKSVLYSTTYDASNFDTWRVDLRNGKRTR
                     ITRGIDWEEDVSTSPDGGSYLQFSSRGFDRMPIFALMPRPPFIEFALFSWVGRYELNQ
                     VNRKCLLEPWLLDSGGERGTYFGQPVDPRPGTGWDNRTLGQWSPDGTRLLMWQARGND
                     DGTPEKPGARIVIADLPARKATGKRSNNTTPVPGWAPARSSYTGFLANSGTFTVYGKK
                     TGSATVSMEGTAFGGSWSVTYDNYSDDGKSFLDGTESTTQASILSGITWTAELTLTGK
                     HSGHLSANLQIIKGEAGAGAQVSGTVSQSLDDRTVSEFPSATCEPLARPRLRVKAARQ
                     KAGRVRVLVTGKVTADPVWRPVRYATVRLGAER"
BASE COUNT          272 a          538 c          586 g          302 t
ORIGIN      
        1 cgctcggcac caagccgcac cgtggcgtac cgcaccggcc gccagaccgg atcggccgtg
       61 accttgccgg taaccagcac gcgaacccgg cccgcctttt gacgggcagc cttgacccgg
      121 agccggggcc ttgccagcgg ctcgcaggtg gcggagggga attccgagac ggtgcggtcg
      181 tccaggctct ggctcaccgt gccggaaacc tgggcaccag cgccggcctc ccccttgatg
      241 atctggagat tcgcgctcag gtggcccgaa tgcttcccgg tcagagtcag ttcggcggtc
      301 caggtgattc cgctcaggat cgatgcctgg gtggttgact cggtgccgtc gaggaaagac
      361 ttcccgtcat cggaatagtt gtcgtaggta acgctccagc tgccgccgaa agcggtgccc
      421 tccatgctga cggtggccga tccggtcttc ttcccgtaca cggtgaaagt gcccgaattg
      481 gcgaggaaac cggtgtagga gcttctggcc ggcgcccatc cgggaaccgg cgtggtgttg
      541 ttactcctct tccctgtcgc cttgcgggcg ggaaggtcgg cgatcacgat tctcgccccc
      601 ggtttctccg gggtgccgtc atcgttgccg cgggcctgcc acatgagcag cctggttccg
      661 tccgggctcc actgccccag ggtgcggttg tcccagccgg tacccgggcg gggatcgacc
      721 ggctggccga agtaagtgcc gcgttcgcca ccggaatcga gcagccacgg ctcgagcagg
      781 cacttccggt tcacctgatt cagctcgtag cgcccgaccc aggaaaagag cgcgaattcg
      841 atgaaaggcg gcctcggcat caacgcgaag atcggcatgc ggtcaaaccc gcgagagctg
      901 aactggaggt atgagccccc gtcgggggag gtggaaacgt cctcttccca gtcgatgccc
      961 ctggtgatcc gggtccgttt gccgttccgg aggtcaactc gccaggtatc gaagttgctt
     1021 gcgtcatagg tggtggagta gaggaccgat ttgccgtccg aggtgaagtt cttgagttcg
     1081 aagtagggag actgggcgcg ccaggccgcc gagtccgtcc ccagcggccc ggaaggattg
     1141 aggatcctga cgttctcgag gtcgtactga cccccgttct ttgccagacg cccgaccgac
     1201 atcgccgtgc cgttcatggt gaactcggtc cagccgaacc agcggccgtc gggcgaaagg
     1261 cgcccctcgc ggttctgggt gccctggtct attccgccgc cgggcatgat gatcggggaa
     1321 agcgtccgct cttcgcagtt gacgagtgtc ggagtgcact ccacgacccc gtaggtgaaa
     1381 ttctgggttg ggctgccccc ggcgccaggc ctcaaaccga gccggaccat cagccgcttg
     1441 ccgtcgggga acggtgccgg cttgccgaag gccgcatcca ccggggcacc gggaacgacg
     1501 gcggtgtcga ggccgcaggt gacgcacttc ggatgtgaac cgtcgaggcg ggtgatcgcc
     1561 agctggggag cggtgaggtc gggtgacgcg acgaccgtca cgaggcgctt tccgtccggc
     1621 atccagtccg gttccgactc gcccgagtac ccggcgggaa cggggaggtg gtgcacggag
     1681 atcggctcaa ggggtgcg
//
LOCUS       DAOQFI010000514         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47102_length_1698_cov_2.950091, whole genome shotgun sequence.
ACCESSION   DAOQFI010000514 DAOQFI010000000
VERSION     DAOQFI010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47102_length_1698_cov_2.950091"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLE93_08740"
     CDS             complement(<1..698)
                     /locus_tag="PLE93_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNH87176.1"
                     /translation="MSERPLEQDFNDDGIKVGPPKKVAAGLPSVAFAMGPMLRNAGPR
                     KTLRSSFTMNHKRGFDCPSCAWISEDRPPAIDFCENGMKSLNSEIAPVTVPREFWAGN
                     SLAGMRDKSEFWLGRQGRITEPVYRPKGSGHYEVIGWEKAFDLIADRLRSLDSPDQAV
                     FYTSGRIMNEPAFLYQLFAREYGTNNLPDCSNMCHEATGAGMGWSIGVGKSTVSYSDF
                     EKADLIMVMGQNPGT"
     gene            complement(695..1585)
                     /gene="fdhD"
                     /locus_tag="PLE93_08745"
     CDS             complement(695..1585)
                     /gene="fdhD"
                     /locus_tag="PLE93_08745"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNH87177.1"
                     /translation="MGRVVSERMVTRFRSGSRPSRRRDRLAGEEPLEIRVNGTPLAVT
                     MRTPGSDYELAAGFLVSEGVISKAADFGGATYCRGGSGPGAANEYNVLDVLLGPGVEP
                     PSPELTRNFYTTSSCGICGKSGIGEIGVDSAFDITGFEGEVELELLLSLPDRLRGSQE
                     VFDETGGLHAAAIFDAASGEMLVSREDVGRHNAVDKVIGWALMEGKLPLTGCVLQVSG
                     RAGFELVQKAKMAGLPVFSAVSAPSTLAVELASASGITLAGFVRGDSLVAYSRPDRIR
                     SVGETPGTAGPDQLPRAVAP"
BASE COUNT          304 a          568 c          535 g          291 t
ORIGIN      
        1 gtgcccgggt tctggcccat gaccatgatc aggtcggcct tttcgaagtc cgaatagctg
       61 accgtcgact taccgacgcc gatcgaccag cccatgccgg ccccggtcgc ttcgtggcac
      121 atgttcgaac agtcgggcag gttgttggtc ccgtattcgc gggcaaatag ctggtagagg
      181 aacgccggtt cgttcatgat ccggccgctg gtgtagaaga ccgcctgatc gggcgaatcc
      241 agcgaacgaa ggcggtcggc gatcagatca aaggccttct cccagccgat cacctcgtaa
      301 tggccggagc ccttcggacg gtagacgggt tcggtgatcc ggccctggcg gccaagccag
      361 aactcggact tgtcgcgcat cccggccagc gagttcccgg cccagaattc ccggggcacg
      421 gtcaccgggg cgatctcgga attgagggac ttcatgccgt tttcgcagaa gtcgatcgcc
      481 ggtggcctgt cctcgctgat ccaggcgcag ctggggcaat cgaagccgcg cttgtggttc
      541 atggtgaagg aggaacgaag ggtcttgcgc ggcccggcgt tcctcagcat cgggcccatc
      601 gcgaaagcga cggagggcaa cccggcggcg accttcttgg gcgggccgac cttgatcccg
      661 tcgtcgttga agtcctgctc cagcggccgt tcgctcaagg cgcgaccgcc cggggaagct
      721 ggtccggccc ggcggtgccc ggggtttctc caacggagcg gattcggtcg ggccggctgt
      781 aggcgaccag tgaatcgccc cggacgaagc cggccagggt tatccccgaa gccgaggcca
      841 gctccaccgc gagggttgac ggtgcggaaa cggccgagaa gaccggcaaa ccggccatct
      901 tcgccttttg gaccagttcg aagccggccc ggccggagac ctggagcacg cagccggtca
      961 gtggcaactt cccttccatc agcgcccagc cgatcacctt gtcgaccgcg ttgtgacggc
     1021 caacatcctc ccgggaaacc agcatttcgc cgctggctgc gtcaaagatg gcagcagcgt
     1081 gaaggccgcc cgtctcatcg aagacctcct ggcttccccg cagccggtcc ggcaggctca
     1141 gcaggagttc aagctcgact tcgccctcga acccggtgat atcgaaggcc gaatcgactc
     1201 cgatctcgcc aatcccggac ttgccgcaga tgccgcagga actcgtcgtg tagaagttcc
     1261 tggtcagctc gggagaaggt ggctccaccc cgggaccgag cagcacgtcg agcacgttgt
     1321 attcgttcgc cgcccccgga cccgaaccgc cacggcaata ggtggcgcca ccgaaatcgg
     1381 ccgccttcga gatcacccct tccgaaacga gaaagccggc cgccagttcg tagtcgctgc
     1441 cgggcgtcct catggtcacc gccagcggcg tgccgttgac ccggatctca agcggctcct
     1501 cgccggccag tctgtcccgg cggcgacttg gccttgaacc ggagcgaaac cgggtgacca
     1561 tgcgctctga aacgactcgg cccattgggt gatcatagat gccaccccgc tccgtcaata
     1621 gcatctgccc cgtgatcaca gtcgccgaac atctcaaccg gatcctcgcc atggtgcccg
     1681 tgctcgaagt cgagaagg
//
LOCUS       DAOQFI010000515         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47269_length_1695_cov_3.157927, whole genome shotgun sequence.
ACCESSION   DAOQFI010000515 DAOQFI010000000
VERSION     DAOQFI010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47269_length_1695_cov_3.157927"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLE93_08750"
     CDS             <1..640
                     /locus_tag="PLE93_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH87178.1"
                     /translation="LSGGQRQRVALARAIVNRPRVLLLDEPLGALDLKLREQMQVELK
                     QIQGEVGITFIYVTHDQDEALTMSDRIAVFNEGRIEQLSSPRDLYEHPGNEFVAGFVG
                     VSNAIERDGRKLTIRPEKIRLIGEGEPADGLVTERGRIAESAYVGMVTRVKVELEQGG
                     AVQVALQNTESEAEPGGRVGQEVVVGWRPEHAVSIQQGERNQSVQLEEEHVS"
     gene            637..>1695
                     /locus_tag="PLE93_08755"
     CDS             637..>1695
                     /locus_tag="PLE93_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH87179.1"
                     /translation="MSYAHRNPARFAWLLVLAMVAVLAVAGCGSSDDDSSSSSDEVTS
                     IGKTEGALNLIAWAGYAEDGSTDKTVDWVTPFEKQTQCQVNTKTASTSDEMVKLMRTG
                     QYDGVSASGDASVRLIEGGDVSPVDMSILSNYADINPSLKDQDYNSKDGQNYGMPHGW
                     GANVLLYNKDAVSPAPDSWGSVFDPAQASKFSGKITAPDNPIYIADAAVYLEATQPDL
                     GITDPYELDEDQFNAAVDLLKQQNKFVGEYWPDATKNISSYTNGDSVIGSSWQYQTSA
                     LQAAGENVDADLPKEGSTGWSDTWMLSSQAEHPNCMLEWMNWMGSPKVQAQVAEWFGE
                     APANLGACDLTSDPKHCDT"
BASE COUNT          341 a          526 c          566 g          262 t
ORIGIN      
        1 gctttccggc ggccagcgcc agcgggtcgc cctcgcccgg gcgatcgtca accgtccccg
       61 ggtgctgctg ctcgacgagc cgctcggcgc cctcgacctg aaactgcgcg agcagatgca
      121 ggtcgagctg aagcagatcc agggtgaggt cgggatcacc ttcatctacg tgacccatga
      181 ccaggacgag gccctgacca tgagcgaccg gatcgccgtc ttcaacgagg gccggatcga
      241 gcagctgagc tcgccccgcg acctctacga acacccgggc aacgagttcg tggccggttt
      301 cgtcggggtg tcaaacgcga tcgagcgcga cggaaggaaa ctgacgatcc ggccggagaa
      361 gatccgcctg atcggtgaag gtgaaccggc cgacggcctg gtcaccgagc ggggccggat
      421 cgccgagagc gcctatgtgg gcatggtgac ccgggtcaaa gtcgaacttg agcagggcgg
      481 tgcggtccag gtcgccttgc agaacacaga gtctgaagcc gagcccggtg ggagagtcgg
      541 ccaggaggtg gtggtcggat ggcggccgga gcacgccgtt tcgatccagc aaggagagag
      601 aaatcagtcc gtccaattgg aggaggaaca cgtttcatga gttatgcgca tagaaatccg
      661 gcccggtttg cctggctgct cgtgctggcc atggtggccg tgctcgcggt cgccggctgc
      721 ggctcgagcg acgacgacag cagcagtagc agcgacgagg tgacctcgat cggcaagacc
      781 gaaggggcgc tcaacctgat cgcctgggcc ggttacgccg aagacggctc gaccgacaag
      841 acggtcgact gggtcacccc gttcgagaag cagacccagt gccaggtcaa caccaagacg
      901 gccagcactt cggatgagat ggtgaagctg atgcggaccg gccagtacga cggtgtctcg
      961 gcctccggcg acgcttcggt gcgcctgatc gagggtggcg acgtcagccc ggttgacatg
     1021 agcatcctct cgaattacgc cgacatcaac ccgagcctga aggaccagga ctacaactcg
     1081 aaggacggcc agaactacgg catgccgcat ggctggggcg ccaacgtcct gctctacaac
     1141 aaggatgcgg tcagtccggc tccggacagc tggggttcgg tctttgaccc ggcccaggcg
     1201 tcgaagttca gcggcaagat caccgctccg gacaacccga tctacatcgc tgacgcggcc
     1261 gtctacctcg aggccaccca gccggatctc gggatcaccg acccgtacga gctcgacgag
     1321 gatcagttca acgccgccgt cgatctgctc aagcagcaga acaagttcgt cggcgagtac
     1381 tggccggatg cgaccaagaa catctcgtcc tacaccaacg gtgactcggt gatcggctcg
     1441 agctggcagt accagaccag tgccctgcag gccgccggcg agaacgtcga tgcggacctg
     1501 ccgaaggaag gttcgaccgg ctggtcggac acctggatgc tgagctccca ggccgaacac
     1561 ccgaactgca tgctcgagtg gatgaactgg atgggttcgc cgaaggttca ggcccaggtc
     1621 gcggagtggt tcggcgaggc gccggccaat ctcggtgcct gtgatctgac ctcggatccg
     1681 aagcactgcg acacc
//
LOCUS       DAOQFI010000516         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47343_length_1694_cov_2.449664, whole genome shotgun sequence.
ACCESSION   DAOQFI010000516 DAOQFI010000000
VERSION     DAOQFI010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47343_length_1694_cov_2.449664"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLE93_08760"
     CDS             <1..606
                     /locus_tag="PLE93_08760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNH87180.1"
                     /translation="DELKAEGIETVFYAVGRRGDGALTFRKAEPKQSWTGFTDRPAFS
                     DAREIGETLASAYVDGEVDRVEVIYNRYVSPLTQHVHRLILLPVQEAEVIGEGTEDEG
                     GEEEGGELAEAHARSSWEYEPEAEQLLATVIPDYVNTSLYRNLLESAASELGSRMTAM
                     RSAAENAETMIDDLTLEMNRVRQAEITQEILEVVGGAEALG"
     gene            641..>1694
                     /gene="atpD"
                     /locus_tag="PLE93_08765"
     CDS             641..>1694
                     /gene="atpD"
                     /locus_tag="PLE93_08765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNH87181.1"
                     /translation="MSESNGNNTGRIEEIQGVVVDVLFPDKLPEIYSAVEIQVKASEG
                     REDQNLICEVQQHLGDDRVRTVAMDATDGLRRGDLVVDTGGPITVPVGDVTLGRIFNL
                     LGEPIDGGDALPADVERWPIHRPSPEATDLTPNQEILETGIKVVDLLAPYVKGGKIGL
                     FGGAGVGKTVLIQELIHNIAQEHGGLSAFCGVGERTREGTDLYIEMTESGVIDKTMMV
                     FGQMNEPPGARLRVALSGLTMAEYYREAGGQDVLLFIDNIFRFTQAGSEVSALLGRMP
                     SAVGYQPTLETEMGQLQERITSTARGSVTSVQAIYVPADDYTDPAPASVFAHLNATTA
                     LSRAISEKGIYPAVDPL"
BASE COUNT          330 a          584 c          525 g          255 t
ORIGIN      
        1 gacgagctga aggccgaagg aatcgagacg gtcttctacg cggtgggccg tcgtggcgac
       61 ggtgccctca ccttccgcaa ggcggagccg aagcagtcct ggaccggttt caccgaccgt
      121 ccggccttct cggacgcccg cgagatcggc gagacgctcg cctcggccta cgtcgacggc
      181 gaggtcgacc gggtcgaagt catctacaac cgctacgtct cgcccctcac gcagcacgtt
      241 catcgtctga tcctgctgcc ggtccaggag gccgaagtca tcggtgaagg aaccgaagac
      301 gaaggcgggg aagaagaggg cggcgagctc gcggaagccc acgcccgctc ctcctgggaa
      361 tacgagccgg aagccgagca actgctcgca acggtcatcc ccgattacgt gaacacttcg
      421 ctttaccgca accttctcga gtcggcagca tccgaactcg ggtcccggat gacggccatg
      481 cgcagcgcgg ccgagaacgc ggaaacaatg atcgacgacc tgaccctgga gatgaaccgg
      541 gtccgtcagg ccgagatcac acaggaaatt ctggaagtcg tgggcggcgc cgaggcgctg
      601 ggttaacgcg acgctaccga ctgaaactgg aactggagat atgtcagaaa gcaacggcaa
      661 caacaccgga cgaatcgagg aaattcaggg cgtcgtggtc gacgtgctct tccccgacaa
      721 actgcccgag atctactcgg ccgtcgagat ccaggtcaag gccagcgagg gccgcgaaga
      781 ccagaacctg atctgcgaag tccagcagca cctcggtgac gaccgggtac gcaccgtggc
      841 catggacgcc accgacggtc tgcgtcgcgg cgacctcgtg gtcgacaccg gcggaccgat
      901 caccgttccg gtcggcgacg tcaccctggg ccgcatcttc aacctccttg gcgagccgat
      961 cgacggtggc gacgcgctgc cggccgacgt cgagcgctgg ccgatccacc gtccgtcccc
     1021 ggaagcgacc gacctgaccc cgaaccagga gatcctcgaa accgggatca aggtcgtcga
     1081 cctcctcgcc ccgtacgtga agggcggcaa gatcggcctg ttcggcggcg ccggcgtcgg
     1141 caagaccgtt ctgatccagg agctgatcca caacatcgcc caggagcacg gcggactctc
     1201 cgccttctgc ggagtcggcg aacggacccg tgagggcacc gacctctaca tcgaaatgac
     1261 cgagtccgga gtcatcgaca agacgatgat ggtcttcggc cagatgaacg agcctcccgg
     1321 agcccgtctc cgcgtcgcgc tgtccggtct gaccatggcc gagtactacc gcgaggccgg
     1381 cggccaggac gtgctgctct tcatcgacaa catcttccgg ttcacccagg ccggctccga
     1441 ggtctcggcc ctgctcggcc gcatgccttc cgccgtcggt taccagccga ccctggaaac
     1501 cgaaatgggc cagctccagg aacgaatcac ctcgaccgcc cgtggctcgg tcacctcggt
     1561 ccaggcgatt tacgtcccgg ccgacgacta caccgacccg gctccggcct cggtgttcgc
     1621 ccacctcaac gcgaccaccg cgctttcgcg ggcgatctcg gagaagggca tctacccggc
     1681 cgtcgatccg ctcg
//
LOCUS       DAOQFI010000517         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47474_length_1691_cov_4.069682, whole genome shotgun sequence.
ACCESSION   DAOQFI010000517 DAOQFI010000000
VERSION     DAOQFI010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47474_length_1691_cov_4.069682"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1691
                     /locus_tag="PLE93_08770"
     CDS             <1..>1691
                     /locus_tag="PLE93_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HNH87182.1"
                     /translation="NLSPDKIDTDFTDGTVLDDDSDATQVGSGPSINKQVSPTVNGNC
                     GSLNDYASGITPVASPGDTICWRLTMSFPNNLTTSNVFVGDYIPPGTTYVFNSTVALP
                     SNTVGIFTGSPPQPDLFGTSGLRWALNDGGGEVQPGKVFDVVFATKVQRSTDSVDGDI
                     EGNLMKAVYTNSDGTSFPLRDEEDFERSQPEISLLKGVYKVNGQPPAGNPPNVDGAVV
                     DGGDDVTYRVDVTNDGSVNAINTQVWDILPPQVTCADVDAGSISNGGTCNAGQNRIEW
                     SGLTVPATGNLTLTYTVEIPDGLSGGDVLNNTAGVREFASSSGSGPYVQVPENNIDPT
                     LDPNANAPAAKDPSNVVIESSTTVKTRVTDINQPGNSPTTQATIGERINYTVTATIPQ
                     GTRLYGPTALVDNVSSRMTVVPLSASATLDNGTGGGPVPLPTAGLTLTQTGNVVKVDF
                     PNPYSNPEHSGDDVIVLTFGAIVRDVYPTNYAQGNSTQYTMPNTGTLSWKDTNGTNRS
                     ASGSVNTTIVEPRISIDKTVDDDGPVSPNDLLNYTIQVSNGSAARVSTANNTVLT"
BASE COUNT          362 a          586 c          483 g          260 t
ORIGIN      
        1 gaacctgagc ccggacaaga tcgacaccga cttcaccgat ggcaccgttc tcgatgacga
       61 ttcggacgcg acccaggtcg ggtccgggcc cagcatcaac aagcaggtaa gcccgaccgt
      121 caacggaaac tgcggcagcc tgaacgacta cgcgagcggc atcaccccgg tcgccagccc
      181 gggtgacacc atctgctggc gcctgaccat gtcgttcccg aacaacctga cgaccagcaa
      241 cgtgttcgtc ggcgattaca tcccgcccgg cacgacctat gtcttcaaca gcaccgtggc
      301 gctgccctcc aacacggtcg gcatcttcac cggctcgccc ccgcagcccg acctgtttgg
      361 tacctccggc ctccgctggg ccctcaacga cggcgggggt gaagtccagc ccggcaaggt
      421 cttcgacgtg gtcttcgcaa cgaaggtcca gcgcagcacc gattccgtgg acggtgacat
      481 cgaaggcaac ctgatgaagg cggtctacac caactccgac ggaacctcgt tcccgcttcg
      541 ggacgaagag gatttcgaaa ggtcccagcc cgagatcagt ctcctcaagg gggtctacaa
      601 ggtcaacgga cagccgccgg cgggcaaccc tccaaacgtt gacggggccg tcgtcgacgg
      661 aggcgatgac gtgacctatc gcgtcgacgt gaccaacgac ggctcggtga acgcgatcaa
      721 cacgcaggtc tgggacatcc tgccgccgca ggtcacctgt gccgacgtcg acgccggttc
      781 gatctcgaac ggcggtacct gcaacgcggg gcagaaccgg atcgaatggt cggggctgac
      841 cgttcccgcc accggcaacc tgaccctgac ttacaccgtt gaaatacctg atggcttgag
      901 cggcggggac gtgctcaaca acactgccgg tgttcgcgag ttcgcttctt ccagtggttc
      961 cggcccctat gtccaggtgc cggaaaacaa catcgacccg accctggatc cgaacgccaa
     1021 cgcgcccgcg gccaaggatc cctcaaacgt ggtgatcgaa tcttcaacca ccgtcaagac
     1081 cagggtcacc gacatcaacc agccgggcaa cagtccgacc acccaggcca cgatcggtga
     1141 gcgaatcaac tacacggtga ccgccacgat tccgcagggc acacgcctct acgggccgac
     1201 cgccctggtt gacaacgtga gctcccggat gaccgtggtt ccgctttctg ccagcgccac
     1261 cctggacaac ggcacgggcg gcggaccggt gccactgccg acggccgggc tcactttgac
     1321 ccagaccggc aacgtcgtaa aggtcgattt ccccaacccg tattcgaacc cggaacacag
     1381 cggcgatgac gtgatcgtcc ttactttcgg ggcgatcgtg cgggacgtct atccgaccaa
     1441 ctacgcccag ggcaactcga cccagtacac gatgccgaat accggaaccc tgagctggaa
     1501 ggacaccaac ggcaccaacc gcagcgcctc cgggtcggtc aacaccacga tcgtcgagcc
     1561 acggatctcg atcgacaaga cagtcgacga tgacggaccg gtgagtccga acgatctgct
     1621 caactacacg atccaggttt cgaacggctc ggcggctagg gtctcgaccg ccaacaacac
     1681 ggtgctgacc g
//
LOCUS       DAOQFI010000518         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47546_length_1690_cov_2.751070, whole genome shotgun sequence.
ACCESSION   DAOQFI010000518 DAOQFI010000000
VERSION     DAOQFI010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47546_length_1690_cov_2.751070"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..825
                     /locus_tag="PLE93_08775"
     CDS             160..825
                     /locus_tag="PLE93_08775"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNH87183.1"
                     /translation="MYVRYMLTEHASSQLVELLGRRRVFSETRRRTKVPGVSTGLAWT
                     PTGGDVLFIEATAMPGNGKLVITGQLGEVMRESAQAALSYVRANARDLAPGLDEKWFA
                     EHDLHIHVPAGAVPKDGPSAGVAITVALVSLITGRPVRNDVAMTGEVTLTGQVLPIGG
                     VKEKSLAAQRAGIKIVILPGRNEGDVEEIPEYERAEVEFFYADKVGDALAVALEGNLK
                     GKQ"
     gene            898..1359
                     /locus_tag="PLE93_08780"
     CDS             898..1359
                     /locus_tag="PLE93_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87184.1"
                     /translation="MGTENISGKASRAYSSARENQHVRRLIEDEELRASLIAAAVAGR
                     KAFQRVQSNRSSAVESVTQDKKVKKELSSAAESLRDAADRLKAPPKKKRHPIRNLLLV
                     GVVTGGLVLVFSESARKSLLDAIFGAEEEFVYTSTTSTETAQTNGTPVGTE"
BASE COUNT          332 a          541 c          533 g          284 t
ORIGIN      
        1 cacctgtcgc ctgggcgtca tcccggatca gtttaaggcg tacgccgcgg cgctcgatga
       61 ccagcggcac gccggtatcg accacctgat cggcgagctt gaacagctgc tggcgcaact
      121 cggtgagcga cacgggcacg gccgcgaccc cctttggaca tgtacgtacg gtacatgctg
      181 acagagcacg cgtcaagcca gcttgtcgag ctgctcggcc ggcgccgggt gttttccgag
      241 acacgacgcc gaaccaaggt tcccggggtt tcgaccggtc tcgcctggac cccgaccggg
      301 ggtgacgtgc tcttcatcga agcaacggcg atgccgggca acggcaagct ggtaataacc
      361 ggacagctcg gagaggtgat gagggaatcg gcgcaggcgg cactttccta cgtgcgggcc
      421 aatgcacggg acctggcgcc gggcctcgac gagaaatggt ttgccgaaca tgacctccac
      481 atccacgtgc cggccggcgc cgtgcccaag gatggccctt cggccggggt ggcgatcacg
      541 gtagcgctgg tttccctgat caccgggcgg ccggttcgca atgacgtcgc gatgaccggc
      601 gaagtgaccc tgaccggtca ggtgctgccg atcgggggcg tcaaggagaa atcgctggcc
      661 gcccagcggg caggaatcaa gatcgtgatc ctgcccggac gaaacgaagg cgacgtcgag
      721 gagattcccg agtacgagcg ggccgaggtc gagttcttct atgcggacaa ggtcggggac
      781 gccctggcgg tcgcgctcga gggcaatctc aagggcaaac aatgagccgg ttcgtttttc
      841 tttgcagcgg ggtaccgcta ttctcgacag acaaccattc gatcaagcga ggaacacatg
      901 ggaacagaga acatctcggg taaggcctcc agggcgtact cgtcggcgcg cgagaaccag
      961 catgtccgtc gcctgatcga ggacgaagag cttcgtgcga gcctgatcgc tgccgccgtg
     1021 gccggtcgca aggcctttca gcgggtccag tcaaaccgga gttccgcggt cgaatcggtg
     1081 acccaggaca agaaggtgaa gaaggaactt tcgagcgcgg cggaatcgct tcgtgatgcc
     1141 gccgaccgcc tcaaggcgcc gccgaagaag aagcgccacc cgatccgcaa cctgctcctg
     1201 gtcggtgtcg tgaccggcgg cctggttctg gtcttcagtg agtcggcccg caagagcctg
     1261 ctcgatgcga tcttcggcgc cgaggaagag ttcgtctaca cctcgacgac ctccaccgag
     1321 accgcccaga ccaacggaac cccggtcgga accgagtaga cccggttccg caaccagttt
     1381 caaagggccc cgggaaaccg gggccctttc tctttgcatc ctcgccggcc cggagtctct
     1441 tctccgaaga ggacgaaact cgacccgctt tcgcgggcaa tcgcggaaac cgcgaggttc
     1501 cattttccgc cacatagcgc acccaactcc gatctcgacc cggtggttca aatcgaagtc
     1561 ggagttgggt gcgctatgtg gcgaaatctc ccttctcgat tctccggcca tcacccgccc
     1621 cacggcaacc caacccatcc atcaaccagt tactgcaggg cccgggtcgg cggtgttccc
     1681 caaaaacctc
//
LOCUS       DAOQFI010000519         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47635_length_1688_cov_3.031231, whole genome shotgun sequence.
ACCESSION   DAOQFI010000519 DAOQFI010000000
VERSION     DAOQFI010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47635_length_1688_cov_3.031231"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLE93_08785"
     CDS             <1..223
                     /locus_tag="PLE93_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNH87185.1"
                     /translation="AREFDLLAYMMRHPGQVLSREQLLNGVWGYDYDPGTNIVGVYIA
                     YLRRKLAMEGRPDPIETLRSVGYRLRDGR"
     gene            226..>1688
                     /locus_tag="PLE93_08790"
     CDS             226..>1688
                     /locus_tag="PLE93_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH87186.1"
                     /translation="MPHPGLRGKLAISIALVLLIALGVTYFAVYRGTGSELKDRTESE
                     LDKGVSQIAARLKVGGADTPAEHTARAHQITSAEGFGPNARLVSISIDGGGTASNQPE
                     LVGPGLEDHHSDGEQKHAGHESGDDSEDEPAEKKQDADRILGSAPGYSTIEVEDAGKV
                     RILTREVELPGGVKATIRVGQPLAPAENALEGLSDTFVIVGIATLLLGALAGWLLAST
                     ATRPMRRMAQVAEGVDAGDLSARMPVEGTRDEARRLAESFNGMLDRLEDAFDRQRAFV
                     ADASHDLRTPLTVVRGQAEVLARNPNPSREEVTRVYELVSQATARMELMVDDLLLLAR
                     AESSEGLRLEPLELEPLISAEVEGMAFENHRDLVAGVVTGRRVNIDREQVARLLSNLI
                     SNAIAHTEEGGRIEVSATDHGSKVVLAVDDDGPGVPESDRERVFDRFARLDESRSSSL
                     GGSGLGLAIVRAIAEAHGGEARCVDSPLGGARFEIGFP"
BASE COUNT          297 a          535 c          593 g          263 t
ORIGIN      
        1 ggcccgtgaa ttcgacctgc tcgcctacat gatgcggcat cccgggcagg tgctttcccg
       61 ggagcagttg ctgaacggag tctggggcta tgactacgac ccggggacca acatcgtcgg
      121 ggtttacatc gcatacctgc ggcgcaagct ggcgatggaa ggccggccgg acccgatcga
      181 aacccttcga tcggtcggct accggctgcg tgacggccgg tgaacatgcc ccatcccgga
      241 ctgagaggca agctcgcgat ctcgatcgcc ctggtcctgc tgattgccct gggggtcacc
      301 tacttcgcgg tctatcgagg caccgggtcc gagctgaagg accgcaccga gtcggagctg
      361 gacaagggag tcagccagat cgccgcgagg ctgaaggtcg gcggcgcgga cacaccggca
      421 gaacacaccg ccagggccca tcagatcacc tcggcggagg gcttcggccc gaacgcccgg
      481 ctggtttcga tctcgatcga cggcggtggc actgccagca accagccgga gctggtcggg
      541 ccgggtctcg aagatcacca ttccgacggc gagcagaaac acgccggcca cgaaagcggt
      601 gacgacagcg aagacgagcc cgccgagaaa aagcaggatg cggaccggat cctggggtcg
      661 gctccgggct actcgacgat cgaggttgaa gacgccggca aggtccggat cctgacccgg
      721 gaggtcgaac tccccggggg cgtcaaggcg acgatccggg tcgggcaacc gctggctccg
      781 gccgagaacg cgctcgaagg cctcagcgat accttcgtca tcgtcggcat cgcgaccctg
      841 ctgctcggcg cgctggccgg ctggcttctg gccagtaccg ccacccgccc gatgcggcgc
      901 atggcccagg tcgccgaagg agtcgacgcg ggagatctct ccgcgaggat gccggtcgaa
      961 gggacccggg acgaagcccg ccggctggcc gaatcattca acgggatgct cgaccgtctc
     1021 gaggatgcct tcgaccgtca acgggctttc gtcgccgacg cttcgcatga cctgcggacc
     1081 ccgctgaccg tggtgcgggg ccaggcggag gtgcttgccc ggaacccgaa cccctcccgg
     1141 gaagaggtca cccgggttta cgagctcgtt tcccaggcga ccgcacggat ggagttgatg
     1201 gtcgatgacc tgctgctgct ggccagggcc gaatcctccg aaggcctccg gctcgagccg
     1261 ctggaactgg agccgctgat ttcggccgag gtggaaggca tggcctttga aaaccatcgc
     1321 gatttggtgg ccggagtcgt caccgggcgc cgggtgaaca tcgaccgcga acaggtcgcc
     1381 cggctgctct cgaacctgat ctcgaacgca atcgcccaca cggaagaagg ggggaggatc
     1441 gaggtgtccg caaccgacca tggatcgaag gtagttctgg ccgtggacga cgacggaccc
     1501 ggagtgcccg agagtgaccg ggagcgggtc ttcgaccgtt ttgcgcggct ggatgagtcg
     1561 cgcagcagtt cgctgggcgg ttccgggctc ggtctggcca tcgtccgggc gatcgccgag
     1621 gcccatggag gtgaagcgag gtgtgttgac tcgccactgg ggggtgcccg cttcgagatc
     1681 gggtttcc
//
LOCUS       DAOQFI010000520         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47744_length_1686_cov_3.026977, whole genome shotgun sequence.
ACCESSION   DAOQFI010000520 DAOQFI010000000
VERSION     DAOQFI010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47744_length_1686_cov_3.026977"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLE93_08795"
     CDS             complement(<1..254)
                     /locus_tag="PLE93_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87187.1"
                     /translation="MTLVDGATLKDSAVRLYSSNVNDAVDVYGSGNVVDSTLTAYRGV
                     YQMNSTGTVEIKRSALIPIDGGAVTEGGTMNIRDSVILNQ"
     gene            complement(251..766)
                     /locus_tag="PLE93_08800"
     CDS             complement(251..766)
                     /locus_tag="PLE93_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87188.1"
                     /translation="MKRALIFMAIGLASIAAFGATQAQANTYCAELPADASCDQNFTG
                     TGTSIQAAVEAAADNPGPDSVKVGPGNFVMPSPVSLSSPGDANKLTLTGSGPSTVLSG
                     DDPQLPDINFSGGTGSSINDLTVSISDDASNEYKVGLALGAGATGSRLTVTFASHLVN
                     GDPSPGKKLLA"
     gene            838..>1686
                     /locus_tag="PLE93_08805"
     CDS             838..>1686
                     /locus_tag="PLE93_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNH87189.1"
                     /translation="MDDAAKKKLEETTRWEPGETEPRIFAEWLEGGYFHPEATGTADQ
                     NYSVAIPPPNVTGVLHMGHALNGSMQDALVRMNRMSGRNTLWILGMDHAGIATQTVVE
                     KRLKSEGISRHEIGREAFTERVWEWKEEFGGSIREQYKRLGASVDYERERFTLDDGYA
                     RAVRKVFTSLYEKGYIYRDHYMVNWDPGSQSAISDLEVVNEEVTDHLYSVDYPVEGSD
                     RVLTVATVRPETMMADTAVAVNPKDERYSDLIGQHCVLPLVGRRLPIIADDHVDVEFG
                     TGALKIT"
BASE COUNT          312 a          520 c          547 g          307 t
ORIGIN      
        1 ttttgattga ggatcaccga gtcacgaatg ttcatcgtgc cgccttcggt cacggcgcca
       61 ccgtcgatcg ggatcagggc cgatctcttg atctccaccg tgccggttga attcatctgg
      121 tagaccccgc ggtaggcggt cagcgtcgaa tccaccacgt tgccggaccc atacacgtca
      181 actgcatcgt tgacgttgct gctgtagagc ctgacggccg agtccttgag ggtggccccg
      241 tccaccagcg tcacgccagt agttttttgc cagggctcgg gtctccgttg acgagatgcg
      301 aggcaaaggt gacggtcagc ctgcttccgg tcgcgccggc acccagcgca agaccgacct
      361 tgtattcgtt gcttgcgtcg tcggaaatgc tgaccgtgag gtcgttgatg ctcgagccgg
      421 tgccgccgct gaaattgatg tcgggaagct gcgggtcgtc gccggaaagg accgtgctgg
      481 gtccggaacc ggtcagggtc agcttgttgg cgtccccggg gctcgagagg gagaccggac
      541 ttggcatcac gaagttgccg gggccgacct tgaccgagtc cggccccggg ttgtcggcgg
      601 cggcctcgac cgcggcctgg atcgaggtgc ccgttccggt gaagttctgg tcgcagctcg
      661 cgtcagcagg aagctcggcg cagtaggtgt ttgcctgcgc ctgggttgcc ccgaatgccg
      721 cgattgacgc aaggccgatc gccatgaaga tgagtgcccg tttcatcaaa ccttcccggt
      781 aagacagtga agtaccgaac accctaaccc ggtggcccgt caataagatc ggattccttg
      841 gacgacgcgg caaaaaagaa gctggaagag accacccgct gggagcccgg cgagaccgag
      901 ccgcggatct ttgccgagtg gcttgaaggc ggctacttcc atcccgaggc gaccggcacc
      961 gccgaccaga actactcggt cgcgatcccg ccgccgaacg tgaccggcgt gctccacatg
     1021 ggtcacgccc tcaacggctc gatgcaggac gccctggtcc ggatgaaccg gatgagcggc
     1081 aggaacacgc tctggatcct cggcatggac catgcgggca tcgccaccca gacggtggtc
     1141 gagaagcggc tcaagtcgga ggggatcagt cgccacgaga tcggccgcga agccttcacc
     1201 gagcgggtct gggagtggaa ggaagagttc ggcggttcga tccgcgagca gtacaagcgg
     1261 ctgggtgcct cggtcgatta cgagcgcgaa cgcttcacgc tcgatgacgg atacgcccgt
     1321 gcggtccgca aggtcttcac ctcgctttac gagaagggct acatctaccg cgaccactac
     1381 atggtcaact gggatcccgg ttcgcagtcg gcgatctccg atctcgaagt cgtaaacgag
     1441 gaagttacgg atcacctcta ttcggtcgat tacccggtcg agggaagcga ccgggtgctg
     1501 accgtcgcca ccgtccgtcc ggagacgatg atggccgaca ccgcggtcgc cgtgaatccg
     1561 aaggacgagc gctacagcga cctgatcggt cagcactgcg ttctgcccct ggtcggacgc
     1621 cggctgccga tcattgccga cgaccacgtt gacgtcgagt tcggcaccgg cgcgctcaag
     1681 atcacc
//
LOCUS       DAOQFI010000521         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47793_length_1685_cov_4.020859, whole genome shotgun sequence.
ACCESSION   DAOQFI010000521 DAOQFI010000000
VERSION     DAOQFI010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47793_length_1685_cov_4.020859"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLE93_08810"
     CDS             <1..670
                     /locus_tag="PLE93_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNH87190.1"
                     /translation="IRLSALMEIPVVHWLTHDSIGLGEDGPTHQPVEQLASLRAMPNL
                     DVIRPADAAEVAEAWKVIMPRTEHPTALVLTRQKVPVIDRSKFADASGLAKGGYVIAD
                     CDGEPELILIATGSEVHIALEAYETLTIEGVKVRVVSLPSWHIFENQDEAYRESILPA
                     AVTKRISIEQASTFGWERYIGEEGVAIGMTTFGASAPFKDLQKYFGFTPDRVVEEAKN
                     LLGS"
     gene            675..>1685
                     /locus_tag="PLE93_08815"
     CDS             675..>1685
                     /locus_tag="PLE93_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87191.1"
                     /translation="MSELRESEAFKTLESHRNDIGDRHLRDLFAEDPSRGTDLVAEAC
                     GLYFDYSKNRITRETIGLLAALARDRRMPERRDAMFRGEHINVSEDRAVLHVALRMPR
                     SRSLVVDGVDVVREVHEVLDRMAAFSESVRSGEWKGQTGKPIRRIINIGIGGSDLGPV
                     MAYEALKDYSRRDLEFRFVSNVDGTDFYEATLDADPAETLFIVSSKTFTTLETMTNAH
                     TARQWLLDGLGGDESAIARHFVAVSTNAEGVSEFGIDTDNMFGFWEWVGGRYSMDSAI
                     GLSTMLAIGPDRFSEMLDGFHAMDEHFLETNLETSLPALHGLLTVWYRNFFDAETHAV
                     FPY"
BASE COUNT          299 a          574 c          526 g          286 t
ORIGIN      
        1 aatccgcctc tcggccctga tggagatccc ggtcgtccac tggctgactc acgattcgat
       61 cggcctcggc gaggacggcc cgacccatca gccggtcgag cagctcgcct cgctccgggc
      121 gatgccgaac ctggacgtga tccgcccggc cgacgcggcc gaggtcgccg aagcctggaa
      181 ggtgatcatg ccgcgcaccg aacatcccac cgccctggtc ctgacccgcc agaaggtgcc
      241 ggtgatcgac cgcagcaagt tcgccgacgc ttccgggctg gcgaagggcg ggtacgtgat
      301 cgccgactgt gacggcgagc cggagctgat cctgatcgcg actggctccg aggtccacat
      361 cgcactcgag gcatacgaaa ccctgaccat cgagggcgtc aaggtccggg tcgtttcgct
      421 gccttcctgg cacatcttcg aaaaccagga cgaggcgtac cgggagtcga tcctgcctgc
      481 cgcggtcacg aagcggatct cgatcgaaca ggcctcgacc ttcggctggg agcgctacat
      541 cggcgaggaa ggcgtggcga tcggcatgac caccttcggc gcgtcggccc cgttcaagga
      601 cctccagaaa tacttcggct tcacccccga ccgggtggtc gaagaagcga agaacctgct
      661 cgggagctga accggtgagc gagctccggg agtcagaagc cttcaagacc ctcgagagcc
      721 accgcaacga cataggcgac cgtcacctga gggatctctt cgccgaggat ccctcccgcg
      781 ggaccgatct ggtcgccgag gcctgcggtc tctacttcga ctactcgaag aaccggatca
      841 cccgtgagac gatcggcctg ctcgctgccc tcgcccggga ccgccgcatg ccggagcgtc
      901 gcgacgcgat gttccggggc gagcacatca acgtctccga ggaccgggcg gtgctgcatg
      961 tggcgctgcg gatgccgcgc agccgctcgc tggtcgtcga cggggtcgac gtggtgcggg
     1021 aagtccacga ggtcctcgac cgcatggccg ccttctccga gtcggttcgt tcgggcgaat
     1081 ggaagggcca gaccggcaaa ccgatccggc ggatcatcaa catcggcatc ggcggatctg
     1141 acctcggccc ggtgatggca tacgaggcgc tcaaggacta ttcccgccgt gacctcgagt
     1201 tccgtttcgt ttctaacgtg gacggcactg acttctacga ggcgaccctt gacgccgacc
     1261 cggccgagac gctcttcatc gtttcctcga aaaccttcac gaccttggag acgatgacca
     1321 acgcccacac cgcccgccag tggctgctcg acggcctcgg cggtgacgag tcggccattg
     1381 ccaggcactt cgtcgcggtc tcgaccaacg ccgaaggagt ctcggagttc gggatcgaca
     1441 ccgacaacat gttcggattc tgggaatggg tcgggggccg ctactcgatg gattcggcga
     1501 tcggcctctc gaccatgctg gcgatcggcc ccgatcgttt cagcgagatg ctcgacggct
     1561 tccacgcgat ggacgagcac ttcctcgaaa cgaatctcga gaccagcctg ccggcccttc
     1621 acggcctgct caccgtctgg taccggaact tcttcgatgc cgagacccac gccgtcttcc
     1681 cctac
//
LOCUS       DAOQFI010000522         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_47817_length_1685_cov_2.595706, whole genome shotgun sequence.
ACCESSION   DAOQFI010000522 DAOQFI010000000
VERSION     DAOQFI010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47817_length_1685_cov_2.595706"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLE93_08820"
     CDS             complement(<1..477)
                     /locus_tag="PLE93_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87192.1"
                     /translation="MAAAMVKTKTPGVYKRGSRYVEVWSYRGTQHKKSHRTYSEARES
                     KARRQAGERRPESRDRFGDYFSEWIQTYAGRTARGFSETTRPEYRRPIEAHALPAWEA
                     WRLTEVEPADVRDLFGKMRKAGSTTSAIKKLRAALSAMFATAVEDGKIRSNPVQGVR"
     gene            complement(1193..>1685)
                     /locus_tag="PLE93_08825"
     CDS             complement(1193..>1685)
                     /locus_tag="PLE93_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87193.1"
                     /translation="SEQRMEAARWGETVSEAEARTRLSALEVEAADVTGSMEAAKVMR
                     EKVELELHYLHVQQFEAFADHAERAGSEALEALEALRPQYEDAVRLWNEARNEWEILV
                     RAHNDGRGGGPGPIIRADGSVRNPDEAPPMLSSVSVCPLRPLVEVFGSEPPRPPELEV
                     EDR"
BASE COUNT          289 a          527 c          501 g          368 t
ORIGIN      
        1 ccggacaccc tgaaccgggt tcgagcggat cttgccgtcc tcgaccgccg tggcaaacat
       61 cgccgagagc gcggccctga gtttctttat cgcggaagtg gtcgatccgg cctttcgcat
      121 ctttccgaac agatcccgca catcggccgg ttccacttcg gtcagccgcc acgcctccca
      181 agctggcagc gcgtgagctt caatcggtcg ccggtactcg ggccgggttg tctcggagaa
      241 tccgcgagcg gtccgtccgg cataggtctg gatccactcg ctgaaatagt cgccgaagcg
      301 gtcccgagat tccggcctgc gctctccggc ctgtcgccga gccttcgact ctcgggcttc
      361 ggaataggtc cggtggcttt tcttgtgctg agtgcctcgg tagctccaga cctcgacgta
      421 ccttgacccg cgcttgtaga cgccaggggt cttggtcttg accatcgcgg cagccatcag
      481 acgccgacct tcagctcaga cttcgacttt gagacgttca ttgccgtacc tccattgttg
      541 gatttggcaa cgggctcact ctgaggcatg accgatagcg ttcggtctgc gtcggggcga
      601 gttccacctt gacccgatgc cacggccccg gagttccagc tcgcggggcc aatattctgc
      661 ccagagcata gctgcaggta ttggattgcg ttacattgcg gttcatgggg aagacaagaa
      721 gttccttctt ggtggctctg cttgctgtcg ttggattgtt ggcatcgggc gcagggacac
      781 cgacagtcgg gtccgcccaa gccagtgtca agccgccgat tgcgctttgg caggttcggt
      841 cagtttcgat gcagaaggcc cgtgactggt ttccatacgg cgcggtcggc aagagcatcc
      901 ggaggtcggt ctgcggatat aggaaccggt ctcgtaccag ggctgtttgt gtcatgagcg
      961 tgcattggac cgaaccaaac ggtctcggtt gcccaaattc gtcgagaccg ggcggtggag
     1021 ttttcggaac gtacaagttc cgggtctact accaaatttt ccagacccat cgggggaata
     1081 ccttctcgac ctggaagcat gtgtattcga aaggccagga taaggtgaac tgcgcagacc
     1141 gggacctttt tctctagcag tggtccactc tcggtacgcc cgggcgtaga gttcaacggt
     1201 cttccacttc gagctccggg ggccgaggcg gttccgatcc aaacacttcg accagcggcc
     1261 gaagagggca gaccgagacg ctcgacagca tcggcggggc ttcgtcaggg tttctcaccg
     1321 acccatcggc ccggatgatc gggccaggac cgccaccgcg accgtcattg tgagcgcgga
     1381 ccagaatctc ccattcgttc cgggcctcgt tccagagccg gaccgcgtcc tcatattgag
     1441 gtcggagcgc ttcaagggct tcgagagctt ccgatcccgc tctctcagca tggtcggcaa
     1501 acgcctcgaa ctgctgaaca tgcaggtagt gaagctccag ctcgaccttt tcccgcatca
     1561 ccttcgcggc ctccatcgaa cctgtcacgt ccgcggcttc cacttcgagg gcggacagcc
     1621 tggtccgggc ttccgcttcg ctgaccgttt cgccccaacg ggccgcctcc attcgttgct
     1681 ctgaa
//
LOCUS       DAOQFI010000523         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48004_length_1682_cov_2.043639, whole genome shotgun sequence.
ACCESSION   DAOQFI010000523 DAOQFI010000000
VERSION     DAOQFI010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48004_length_1682_cov_2.043639"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLE93_08830"
     CDS             complement(<1..673)
                     /locus_tag="PLE93_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNH87194.1"
                     /translation="MSARADRLAKRLTEAGPDDSAIDALLVTNLTNVEYLTGFTGTNG
                     ACLVSGERQVFLTDFRYAERAAGIEGWEVEIVSGQWLEGLASRLRGRTGFEDDHVTVR
                     TANLLREAAAEAELIEAGGQVERLRRVKDADEIERIAAAAAMTDELYGLLIERGMVGQ
                     TEAEVDGWLTGRMRDQGAEPSFPPIVASGINGASPHAEPGPREIGPGELVTVDMGVKL
                     DGYCSD"
     gene            complement(670..1119)
                     /locus_tag="PLE93_08835"
     CDS             complement(670..1119)
                     /locus_tag="PLE93_08835"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNH87195.1"
                     /translation="MSAATNRIAVLHGVNFDVLERRDPELYGGLSLAELEYKIGGFAG
                     EAGLEPIFFQSNSEAEFCGYLHRLAETADGAIINAGAWTHYSRAIGDAFGVEPVPAVE
                     VHLSDVESREDWRKVSVFDGLVEAKISGKGVDGYREALEHLANRLGV"
     gene            complement(1136..>1682)
                     /locus_tag="PLE93_08840"
     CDS             complement(1136..>1682)
                     /locus_tag="PLE93_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNH87196.1"
                     /translation="TLPAAELTAGMAEVVKTALLAGGGLWEEVAALEPGEILTRADLV
                     FACARYKCSVVAADERDTGLRAQLNLGHTVGHAIESVTSYERYRHGEAIALGLLAALR
                     LSGAGQLREEVREWNLRHGLPVELDPSIDPGAVVEAVQFDKKKTSRGVGFVLLEVPGQ
                     PLIDRPVEGSALLASVEELLP"
BASE COUNT          274 a          582 c          546 g          280 t
ORIGIN      
        1 agtcggagca gtagccgtcg agcttcacgc ccatgtcgac cgtgaccagc tcgcccggcc
       61 cgatctcgcg gggtccgggt tccgcatgcg gcgaggcgcc attgattcct gacgcgacga
      121 tcggcgggaa ggaaggttcg gcgccctggt cgcgcatccg gccagtcagc cagccgtcga
      181 cctctgcctc ggtctggccg accatcccgc gctcgatcag caacccgtaa agctcatcgg
      241 tcatcgcagc ggcggcagcg atcctttcga tctcgtccgc gtccttcacg cggcgcaggc
      301 gctcgacctg accgccggcc tcgatgagct ccgcctcggc cgcggcttcg cgcaacagat
      361 tcgcggtgcg aacggtgacg tgatcatctt cgaagccggt ccggccccgg agcctcgaag
      421 cgagcccttc gagccactgg ccggaaacga tttcaacttc ccacccttcg atcccggccg
      481 ccctttcggc gtagcggaag tcggtcagga agacctgccg ttcgccggag acgaggcagg
      541 ccccgttggt gccggtgaag ccggtcaggt actcgacgtt ggtgaggttg gtgaccagca
      601 gggcatcgat cgccgaatcg tccgggcccg cctcggtgag gcgtttcgcg agacgatcgg
      661 cgcgagcact catacgccga gtcggttggc caggtgctcg agggcctcgc ggtagccgtc
      721 gactcccttg ccggagatct tcgcctcgac caggccatcg aaaaccgaga ccttgcgcca
      781 gtcctcgcgg ctttccacat cggaaagatg tacctcgacc gccggcaccg gttcgacccc
      841 gaaggcgtcc ccgattgccc ggctgtaatg cgtccacgcc ccggcattga tgatcgcgcc
      901 gtcggccgtc tcggccaggc gatgaaggta gccgcagaac tccgcctcgg aattcgactg
      961 gaagaagatc ggttcgagac cggcctcgcc cgcgaagccg ccgatcttgt actcgagctc
     1021 ggccaggctg agaccgccgt agagctccgg atcccgtcgc tcgagcacgt cgaagttgac
     1081 gccgtgaagg actgcaatgc ggttggtggc tgcgctcaac ggcgaccttt cgggttcatg
     1141 gcagaagctc ctcgaccgag gcaagaagcg cgctgccttc gactggacgg tcgatgaggg
     1201 gctgtccggg gacttccaag agcacgaagc ctactccgcg cgaagtcttc ttcttgtcga
     1261 actgcaccgc ctcgaccacc gctccgggat cgatcgaggg atcgagctcg accgggagcc
     1321 cgtgacgaag gttccattcg cggacctctt cccgcagttg gcccgccccg gaaaggcgga
     1381 gcgctgccag cagcccgaga gctatcgcct caccgtgccg gtagcgctcg taggaagtga
     1441 ccgattcgat cgcgtgaccg accgtatggc cgaggttgag ctgggcccgg agaccggtgt
     1501 cccgttcgtc cgcggcgacg accgagcact tgtagcgggc acaggcgaag accagatcgg
     1561 cccgggtcag gatctccccc ggctcgagcg cggcgacctc ctcccacagc ccgcctccgg
     1621 cgagcagcgc ggtcttgacg acctcggcca tgcctgcggt cagctcggcc gcgggcaggg
     1681 tc
//
LOCUS       DAOQFI010000524         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48059_length_1681_cov_1.936039, whole genome shotgun sequence.
ACCESSION   DAOQFI010000524 DAOQFI010000000
VERSION     DAOQFI010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48059_length_1681_cov_1.936039"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLE93_08845"
     CDS             <1..647
                     /locus_tag="PLE93_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNH87197.1"
                     /translation="APHDYSVAVQRAGGIALLLPTDRAVTEDPGPLLDRLDGLILGGG
                     ADVDPGSYESEAGPLTAGTRPERDQFELALTRGALERDLPLLGVCRGFQLMNVAAGGT
                     LEQHLPDVVGHERHRSVPGEFDQHEDELAAGSRIARICGKDRLTVHSHHHQGLARIAE
                     AIEVTGVAVPDRVTEAIELPGREFALGVQWHPEVDEDDSLIAALVEAARGRMSA"
     gene            647..>1681
                     /locus_tag="PLE93_08850"
     CDS             647..>1681
                     /locus_tag="PLE93_08850"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH87198.1"
                     /translation="MISVVEPATEQVMAELPRAGVEEVDAAVAAARAAFPAWRAVTPG
                     DRATLMRRIADGVEARLEEIATLEARNAGKPISDARGEIGMVVETFRYYAGAPERLLG
                     HTIPVAGGVDFTFREPMGVIGLIVPWNFPLAITSWKVAPALAAGNTIVLKPAELTPLT
                     ALELERVALEAGLPEGVLNVVPGPGRTCGNRLVEHPDVNKIAFTGSTEVGRGIMAGAA
                     QTIKRVTLELGGKSATVVFADADIEAAAAAAPAAVFGNAGQDCCARSRILVERSRVDD
                     FLAAMQSTVNDLKVGDPLDEATQMGPLISSSHRETVASFVPEDAPVAFRGSVPDGPGF
                     WFAPTVLSPVS"
BASE COUNT          261 a          608 c          569 g          243 t
ORIGIN      
        1 tggcaccgca cgactactcg gtggcggtcc agcgggccgg agggatcgcc ctgcttctgc
       61 cgaccgaccg ggcggtcacc gaagatcccg gtccgctgct cgaccgtctc gacggcctga
      121 tcctgggggg cggcgccgac gtcgaccccg gctcctacga gtccgaagcg ggaccgctta
      181 ccgccggcac cagacccgag cgggatcagt tcgagcttgc cctgacccgc ggcgcactcg
      241 agcgggacct gccgctcctc ggggtctgcc gcggtttcca gttgatgaac gtggcggcgg
      301 gcggaacgct tgagcagcac ctccccgatg tcgtcggcca cgagcggcac cgctcggttc
      361 cgggcgagtt cgaccagcac gaggacgagc tggccgccgg ctcgcggatc gcgcggatct
      421 gtggcaagga ccggctgacc gtccactccc atcaccatca gggtctggcc cggatcgccg
      481 aggcaatcga agtgaccggc gtcgccgttc cggatcgggt cacggaagcg atcgagctgc
      541 ccggaaggga gttcgcgctg ggggtccagt ggcaccccga agtcgacgaa gacgattcgc
      601 tgatcgcggc tctggtcgag gccgcccgtg gcagaatgag cgcctgatga tttccgtagt
      661 cgaacccgca accgaacagg tcatggccga actgccccgg gccggagtcg aagaagtcga
      721 cgccgccgtg gccgccgccc gcgccgcctt ccctgcctgg cgcgcggtca cccccggcga
      781 tcgggcgacg ctgatgcgca gaatcgccga cggggtcgaa gcccggctgg aggagatcgc
      841 gaccctcgag gccaggaacg ccggcaaacc gatcagcgac gccaggggcg agatcggcat
      901 ggtcgtcgag accttccgct actacgcggg cgcacccgag cggctgctcg gccacaccat
      961 cccggtcgcc ggaggagtcg acttcacctt ccgtgagccg atgggcgtga tcggcctgat
     1021 cgtgccctgg aacttcccgc tggcgatcac ctcctggaag gtcgccccgg cgctcgccgc
     1081 cggcaacacg atcgtgctga aaccggccga actgaccccg ctcaccgccc tcgaactcga
     1141 acgggtcgcg ctcgaagccg gcctgcccga aggcgtgctg aacgtggtgc ccgggccggg
     1201 ccggacctgc ggcaaccggc tggtcgagca cccggacgta aacaagatcg cattcaccgg
     1261 ttcgaccgag gtcggccgcg ggatcatggc cggcgccgcg cagaccatca agcgcgtcac
     1321 gctcgaactc ggcggcaagt cggccacggt cgtcttcgcc gacgccgaca tcgaagccgc
     1381 ggccgccgcg gctcccgcag ccgttttcgg caacgccggc caggactgct gcgcccgatc
     1441 acggatcctg gtcgaacggt cgcgggtcga cgacttcctg gcggcgatgc agtcgaccgt
     1501 gaacgacctc aaggtcgggg atccgctcga cgaggcgacg cagatgggac ccctgatctc
     1561 ctcgtcgcac cgcgagaccg tcgcttcgtt cgtacccgaa gacgctcccg tcgcgtttcg
     1621 cggcagcgtc ccggacggcc cgggtttctg gttcgcaccc accgtccttt cgccggtctc
     1681 c
//
LOCUS       DAOQFI010000525         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48080_length_1680_cov_3.380923, whole genome shotgun sequence.
ACCESSION   DAOQFI010000525 DAOQFI010000000
VERSION     DAOQFI010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48080_length_1680_cov_3.380923"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1680
                     /locus_tag="PLE93_08855"
     CDS             <1..>1680
                     /locus_tag="PLE93_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87199.1"
                     /translation="DACDKGFIHLMDTRYEVRDAQYFAGLLADEDLIQPKKIGTAGAS
                     YGGAKSISLGALRNRVMLPDGSLIPWQSPEGKDMEIAVAAPIVPPSDFAYSLVPNGRT
                     YDYTRFNTYLGPGGDAPFGVMKSAITNGLASAGDSFSGEHGTDLDPLWDINGWKVMMN
                     AGEPVDTAVATTMTDEMTTHHSAYYIDHSIEPSPMIIAQGLTDDLFPISESIRFYNRT
                     KSEYPNSTVGLLFADIGHPRAPLEEPNEQGRPADMELGYTTVEKWFDYYLNGTLPKPD
                     DGVTAMSMVCPYSEPSGGPYTSDTWADFAPGEMRLSDPAKRVIAADGGDDNVASNFMT
                     LIPACTQQQETEEPGIAQYDFPVSSGAGYTLAGSATIIADVSVANGGESQIAGRLLEI
                     TPDGNQRLIARGVYRPESSGTQVIQLNANVYHFAPGTKMRIQLLPRDGSTGLPQLSSF
                     RPSNDQQDVTIKNVDIRVPVREEPGSLNGLVKSPSPKVLPDGYELATGFSSIGSISMA
                     DYIGNRDAKPGKLKVTAATVKGRQLTAKVSCPAAAGSCAKAKLSFKGAAGRG"
BASE COUNT          324 a          582 c          518 g          256 t
ORIGIN      
        1 gacgcctgtg acaagggctt catccatctg atggacaccc gctacgaggt cagggatgcg
       61 cagtacttcg ccggcctgct cgccgacgag gatttgatcc agccgaagaa gatcggcacg
      121 gccggagcct cctacggcgg cgccaagtcg atctcgctgg gcgccctgag gaaccgggtg
      181 atgctccccg atggttctct catcccgtgg cagagcccgg agggcaagga catggagatc
      241 gcggtcgccg ccccgatcgt gcccccgagc gacttcgcct actccctggt gccgaacggc
      301 cggacctacg actacacccg cttcaacacc tacctcggcc ctggcggcga cgcaccgttc
      361 ggagtcatga agtccgcgat caccaacggc ctcgcctcgg ccggcgactc cttcagcggc
      421 gagcacggca ccgacctcga tccgctctgg gacatcaacg gctggaaggt catgatgaac
      481 gcgggcgagc cggtcgatac ggcggtcgcc acgacgatga cggatgagat gaccacccat
      541 cactccgcct actacatcga tcacagcatc gagccgtcac cgatgatcat cgcccagggg
      601 ctgaccgacg atctcttccc gatcagcgag tcgatccgtt tctacaaccg gaccaagagc
      661 gagtacccga actccactgt cggtctgctg ttcgccgaca tcggccatcc ccgggccccg
      721 ctggaggagc ccaacgagca gggacgcccg gctgacatgg agctgggtta cacgaccgtg
      781 gagaagtggt tcgactacta cctcaacggc accctcccga aacccgatga cggggttacc
      841 gcgatgagca tggtctgccc gtacagcgag ccttccggcg ggccttacac gtccgatacc
      901 tgggccgact tcgcccccgg tgagatgcgg ctcagcgacc cggccaagag ggtgatcgcc
      961 gccgacggtg gcgacgacaa cgtggccagc aacttcatga ccctgatccc ggcctgcacc
     1021 cagcagcagg aaaccgagga accgggaatc gcccagtacg acttccctgt ttcgagcggg
     1081 gcgggataca cgctggccgg ttccgcgacc ataatcgccg acgtttcggt ggccaacggt
     1141 ggcgagtcgc agatcgccgg ccggctgctt gagatcaccc cggacggcaa ccagcgacta
     1201 atcgcccgcg gcgtctaccg accggagagc tcgggcaccc aggtcatcca gctgaacgcc
     1261 aacgtgtacc acttcgctcc gggcacgaag atgcggatcc agctgctgcc tcgggacggc
     1321 agcaccgggt tgccgcagct ctcctctttc cgcccgtcga acgatcagca ggacgtgacg
     1381 atcaagaacg tcgacatcag ggttccggtg cgggaagagc ccggctcgct gaacggcctg
     1441 gtcaagagtc catccccgaa ggtcctgccc gacggctacg agctggcgac cgggttcagc
     1501 tcgatcggat ctatcagcat ggctgactac atcggcaacc gcgacgccaa gccgggcaag
     1561 ctgaaggtga ccgctgccac ggtcaagggc aggcagctca ccgccaaggt cagttgcccg
     1621 gccgcggccg gctcctgcgc gaaggcaaag ctttccttca agggcgccgc cggcaggggc
//
LOCUS       DAOQFI010000526         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48134_length_1679_cov_3.556650, whole genome shotgun sequence.
ACCESSION   DAOQFI010000526 DAOQFI010000000
VERSION     DAOQFI010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48134_length_1679_cov_3.556650"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLE93_08860"
     CDS             complement(<1..459)
                     /locus_tag="PLE93_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87200.1"
                     /translation="MSTFATGNGGWTSSTDYGGVCVPAVTCPELSGSYQSSGGAAGGS
                     DGYIRTDSGELTVASLLSTSTHTWRSPAFTYNGVGGAQPEGLHFSVGVNPAVAQLLNL
                     GVDIGISARFTGVSGGGDQIVIDDVTPGTASGWKTLNGQIGPDALEVGKRY"
     gene            452..595
                     /locus_tag="PLE93_08865"
     CDS             452..595
                     /locus_tag="PLE93_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87201.1"
                     /translation="MLTFPLGYVTVAAFAPAGISQAISIDNAAIAAAGKSLLRIMLIS
                     SLH"
     gene            complement(737..1258)
                     /locus_tag="PLE93_08870"
     CDS             complement(737..1258)
                     /locus_tag="PLE93_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNH87202.1"
                     /translation="MESLKGRLLVAAPSLFDFFRRTVVLVVEHTDQGAFGVILNRRAD
                     TEVAELVPDLGPLVEQGAPLWVGGPVGPDSIVVLGDFNHPDQSAGVVEGSIGVVDPDG
                     DADFSRARVFVGHAGWGPGQLEGEVERDSWIVSDFDPDDVFSEGDLWAEVVRRPGGEL
                     AILATMPDDPTLN"
     gene            1365..>1679
                     /gene="rpsD"
                     /locus_tag="PLE93_08875"
     CDS             1365..>1679
                     /gene="rpsD"
                     /locus_tag="PLE93_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNH87203.1"
                     /translation="MGRYTGPKEKLSRREGVDLGLKGERALGGKSAMERRPYPPGQHG
                     RGRRRASEFSVRMRAKQRAKAMYGLREKQFAKLFDRANREEGMAGENLMRHLESRLDN
                     VVY"
BASE COUNT          310 a          562 c          531 g          276 t
ORIGIN      
        1 gtatcgcttg ccgacctcga gcgcgtcggg cccgatctgg ccgttcagcg tcttccagcc
       61 ggaggcggtg ccgggggtga catcgtcaat cacaatctga tctccaccgc cggaaacgcc
      121 ggtgaacctg gcgctgatgc cgatgtcgac acccaggttc agcaactgcg ccaccgcggg
      181 attgaccccg accgagaagt gcagcccttc gggctgtgcg ccgccgaccc cgttataggt
      241 gaaggcgggg ctccgccagg tgtgggtgga ggttgaaagc agggaggcga cggtaagttc
      301 gccggaatcg gtccggatgt acccgtcgct gccgccggct gcgccaccgc ttgactggta
      361 gctgccggaa agctcgggac aggtcactgc cggcacacat actccgccgt agtcggtgct
      421 cgaggtccag ccaccgttgc cggtggcgaa agtgctcacg ttcccgctcg gatatgtcac
      481 ggtggccgcc ttcgccccgg ccgggatcag ccaggcgatc agtatcgaca acgccgcgat
      541 tgccgcagca ggcaagagtc tgctccgcat aatgctcatc tcttctctcc attgagttcg
      601 ggttggcgat ccgtgccgtt cggcccaggg ggatcgctta ccgttcaatc gggtgacggc
      661 gttaccgcca ccgccaacgg aaccactatt cccgaagcca ccgcttcgca aacagggtgt
      721 tccggtcctc ttgctttcag ttcagagtcg ggtcatccgg catcgtggcg aggatcgcca
      781 gttcgccccc cggccggcga acgacctcgg cccagagatc gccttccgag aagacatcat
      841 cgggatcgaa gtcggaaacg atccaggaat cgcgctcgac ttcaccttcg agctgtcccg
      901 gcccccagcc ggcgtgtccg acgaagacac gggcccggga gaagtcggca tcaccgtccg
      961 ggtcaaccac cccgatgctg ccctcgacca ccccggccga ctggtccgga tggttgaaat
     1021 ccccgagcac gacgatcgag tcggggccga ccgggccacc gacccagagc ggagccccct
     1081 gctcgaccag cggcccgaga tcgggaacca gttcggccac ctcggtgtcg gcgcggcggt
     1141 tgagaatcac cccgaaggcc ccctgatcgg tgtgttcgac gaccagcaca acggtgcgcc
     1201 gaaagaaatc gaaaaggctc ggtgcggcta ccagcaagcg tcccttgagc gactccatgt
     1261 accgactata ggacgctgtt cggcgtcgag tggacgcgga ccggccatgg cgctgccgca
     1321 caaagcggga attcgcccga tccggtaggc tccttcgccg cactatgggc agatacacag
     1381 gaccaaaaga aaagctttcg cgccgtgagg gcgtagatct cggactcaag ggtgaacggg
     1441 ccctcggcgg caagagcgcc atggagcgtc gcccctaccc gcccggccag catggccgcg
     1501 gccggcgccg tgcttcggag ttctcggtcc gtatgcgtgc caagcagcgc gccaaggcga
     1561 tgtacggcct tcgcgagaag cagttcgcca agctcttcga ccgcgccaac cgtgaagaag
     1621 gcatggccgg cgagaacctg atgcgtcacc tcgagagccg tcttgacaac gtcgtttac
//
LOCUS       DAOQFI010000527         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48142_length_1679_cov_3.275862, whole genome shotgun sequence.
ACCESSION   DAOQFI010000527 DAOQFI010000000
VERSION     DAOQFI010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48142_length_1679_cov_3.275862"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PLE93_08880"
     CDS             complement(<1..1032)
                     /locus_tag="PLE93_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNH87204.1"
                     /translation="MNPTRPATGPARLKLRAFLFAALAGFVLFLTAGDSAGAAPKLGY
                     TRIATAREPTALVSPPGYPRMLFVTERAGFVRVIANGRKLKQPLLDLTSKVGSSNIER
                     GLIGLAFAPDFRTSGRFYVDYTAKNGNSIVAGYRTVPGQPTRLARGGGRLVIQIPRVN
                     NKGNHNGGHLAFRGNLLYIAVGDGFDPGDAPDHARNLNSLRGKILRIDPRPDQGTGKP
                     YRIPAGNPFVGLPGRDEIFAYGLRNPFTFSFFNSGGEAMMSIADVGQLRYEELNVLPF
                     AEARGANFGWKGFEGFEPYNCGESLCPNGADPVVATGLKWPQLVYSHDEGCAIVGGLK
                     ISDPKLTAVR"
     gene            complement(1029..>1679)
                     /locus_tag="PLE93_08885"
     CDS             complement(1029..>1679)
                     /locus_tag="PLE93_08885"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNH87205.1"
                     /translation="LYFGTGDGGGAGDEPGNAQNLNSLLGKLIRIDPRRSGKRPYTVP
                     ASNPFVGRRGRDEIFAYGLRNPFRWSFDRTSSKRNVRIAIGDVGQDAFEEVNYLNLSR
                     ARGGNFGWNRWEGYTPFNGGGAGTIKPSLVLPHPPNCSVIGGLVVRDRRLPALRGRYV
                     FTDFCSGKILSFKPHLGRAAGRPTGLNIGQVTSFGDSLGGALFVTSLNGSVYRIRQ"
BASE COUNT          286 a          520 c          597 g          276 t
ORIGIN      
        1 cctgaccgcc gtcagtttcg ggtcgctgat cttcaggccg ccgacgatcg cacagccctc
       61 gtcgtgggag tagacgagct gaggccattt caggccggtg gcaaccaccg ggtcggcgcc
      121 gttcgggcaa agtgactcgc cgcagttgta gggctcgaag ccctcgaaac ccttccagcc
      181 gaaattggcc ccccgcgctt cggcgaaggg gaggacgttc agctcctcgt agcgaagctg
      241 gccgacgtcg gcgatcgaca tcatcgcctc gccgccggag ttgaagaagc tgaaggtgaa
      301 ggggttgcgc agcccgtagg cgaagatctc gtcgcgtccg ggaaggccga cgaagggatt
      361 gccggccggg atccggtacg gctttccggt cccctgatcc ggccgggggt cgatccgcag
      421 gatcttgccg cgcaggctgt tcaggttccg ggcgtgatcc ggggcgtcac ctgggtcgaa
      481 gccgtcaccg accgcgatgt agagaagatt gccgcgaaag gcgaggtggc ctccgttgtg
      541 gttgcccttg ttgttgaccc gcgggatctg gatcacaagc cggccgccgc cccgcgcaag
      601 ccgggtcggc tggccgggaa cggtccggta cccggccacg atcgagttcc cgttcttcgc
      661 cgtgtaatcg acgtagaagc ggccgctggt ccgaaaatcc ggggcgaaag cgagtccgat
      721 cagacccctt tcgatgttgc tcgaaccgac cttcgaagtg aggtcgagca ggggctgctt
      781 cagcttcctg ccgttggcga ttacgcgaac gaaaccggcc cgctcggtga cgaagagcat
      841 ccgcgggtag ccgggcgggg aaacgagggc ggtcggttcc ctggcggtcg cgatcctggt
      901 gtaaccgagt ttcggtgcgg cgccggccga atcgcccgcg gtcaggaaga gaacgaaacc
      961 ggccagggcc gcgaacagga acgcccggag tttcaatcgg gccgggcccg tcgctggccg
     1021 ggtcggattc actgcctgat ccggtagacg ctgccattca gcgaagtgac gaaaagcgca
     1081 ccgccgaggc tgtcaccgaa agaggtgacc tggccgatgt tgaggccggt cgggcggccg
     1141 gccgcccggc cgagatgagg tttgaaactg aggatctttc cgctgcagaa atccgtgaac
     1201 acgtaccgcc cgcgcagggc cggcaggcgg cggtcacgaa ccacgaggcc accgatcacc
     1261 gaacagttgg ggggatgggg gaggaccagc gagggcttga tcgtcccggc cccgcctccg
     1321 ttgaaggggg tgtaaccctc ccagcggttc cagccgaaat tgccgcccct ggcccggctc
     1381 aggttgaggt agttgacctc ctcgaatgcg tcctggccga cgtcgccgat cgcgatccgg
     1441 acgttgcgct tcgagctggt ccggtcgaag gaccagcgga agggattgcg gaggccgtag
     1501 gcgaagatct cgtcccggcc gcgacgaccg acgaacgggt tgctggcggg caccgtgtag
     1561 ggacgctttc cgctgcggcg ggggtcgatc ctgatcagct tgccgagcag cgagttgagg
     1621 ttctgggcgt tgccgggctc gtcacccgcg ccgccaccgt caccggtgcc gaagtaaag
//
LOCUS       DAOQFI010000528         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48258_length_1677_cov_3.599260, whole genome shotgun sequence.
ACCESSION   DAOQFI010000528 DAOQFI010000000
VERSION     DAOQFI010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48258_length_1677_cov_3.599260"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /gene="moaC"
                     /locus_tag="PLE93_08890"
     CDS             complement(<1..430)
                     /gene="moaC"
                     /locus_tag="PLE93_08890"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNH87206.1"
                     /translation="MTFTHLDESGQARMVDVTAKQPTVRSATASGFVRCPPAVIAALR
                     DEDLPKGDALAVARVAGIQGAKKCAELLPLAHVIGVHGAAVSFETGDEGVSVSATVRT
                     ADRTGVEMEALTAVSVAALTLIDMFKGIDKGVTIESVRLEE"
     gene            complement(427..>1677)
                     /locus_tag="PLE93_08895"
     CDS             complement(427..>1677)
                     /locus_tag="PLE93_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNH87207.1"
                     /translation="PVITVTEHLNRILAMVPVLEVEKAGLAEARGRFLASPVTAAIPV
                     PPWTNSAMDGYAVRFDDLAGASQQSPVTLRVVGDLPAGTGEDPAIGPGEAVRIMTGAP
                     VPAAADTVIPQEMTDGGLEEVRIEEQLPRGRHIRREGEDRAQGDQVSPAGIELSPESL
                     AAMASAGIGEVIVARRPRVAVISTGDELVAPGEPVLRGRIPDSNGLLVTMLAADAGAL
                     AEADHAGDGPGQLEAALGRNGGCDALVLTGGVSVGAFDPVKALFEGDESVRFDRVAMQ
                     PGKPQAFGRLGESGPLVFGLPGNPVSAWVSFQMFVRPALRKMQGAARLFPEAVPARAT
                     EDWKTPQGRMQVIPVRISDENERKVLPAATGGSGSHLIASLAAADGYALVEPEVEKIK
                     AGDLVPTVRLRDPEQFNHPQRRQP"
BASE COUNT          253 a          599 c          556 g          269 t
ORIGIN      
        1 tctcttcgag gcgaaccgat tcgatcgtca cgcccttgtc gatccccttg aacatgtcga
       61 tcagggtgag ggcggcaacc gagaccgcgg tcagggcctc catctcgact ccggtccggt
      121 cggcggtgcg caccgtcgcg ctgacgctca ccccttcgtc cccggtctcg aatgacaccg
      181 cggcaccgtg cacgccgatc acgtgggcca gcggcaggag ctcggcgcat ttcttggccc
      241 cctggatacc ggccacccgg gccacggcca gcgcatcacc cttcggcaag tcctcatcgc
      301 gcagggccgc aatcaccgct ggcgggcagc gcacgaaacc tgaagccgtg gccgaccgca
      361 cggtaggctg cttggcggtc acgtcgacca tccgggcctg gccgctttcg tctaggtggg
      421 tgaaggtcaa ggttgtctcc tctgggggtg gttgaactgc tccgggtcac gcaggcggac
      481 ggtcggtacg agatcgccgg ccttgatctt ctcgacctcg ggctcgacca gggcgtaacc
      541 gtcggccgcc gccaggctgg cgatcaggtg ggagccggag ccaccggtcg cagccggcag
      601 cacctttcgc tcgttctcgt cggagatccg caccggaatc acctgcatcc ggccttgcgg
      661 ggtcttccag tcctccgttg cccgggccgg gaccgcctcg ggaaagagcc gggccgcccc
      721 ctgcatcttt cgcagggcgg gacggacgaa catctgaaag gaaacccagg cgctgaccgg
      781 gttgccgggc agcccgaaga cgagcggtcc gctttcaccc agtcggccga aggcctgcgg
      841 tttgccgggc tgcatcgcaa cccggtcgaa gcggaccgat tcgtctcctt cgaagagtgc
      901 cttgaccggg tcgaaggctc cgacactgac cccgccggtc aggaccaatg cgtcgcagcc
      961 accgttccgg ccaagcgccg cctcgagttg accgggcccg tcgccggcgt ggtcggcctc
     1021 ggccagggca ccggcgtcgg cagccagcat cgtgacaagc aggccattcg agtccgggat
     1081 ccggccgcgc aaaaccggtt cgccgggagc gacaagttca tcgcccgtgc tgatcaccgc
     1141 cacgcgtggc ctgcgggcga cgatcacctc gccgatgcca gccgaggcca tggcggccag
     1201 agattccggc gaaagctcga ttccggccgg cgagacctga tcaccctggg cccggtcctc
     1261 gccctcccgg cggatgtggc ggcccctggg cagctgttcc tcgatccgga cctcttcgag
     1321 gccaccgtcg gtcatctcct gcgggatgac cgtgtcggcc gcggccggga ccggtgcgcc
     1381 ggtcatgatc cgcaccgctt caccgggccc gatcgccggg tcctcgccgg tgccggccgg
     1441 gaggtcgccg accacccgca aagtcaccgg tgactgttgg ctcgctccgg ccagatcgtc
     1501 gaagcgcacg gcgtatccat ccatcgccga attggtccat ggcggcaccg gaatcgccgc
     1561 ggtgacggga cttgccagaa aacggccgcg cgcttccgcc agacccgcct tctcgacttc
     1621 gagcacgggc accatggcga ggatccggtt gagatgttcg gtgacggtga tcacggg
//
LOCUS       DAOQFI010000529         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48310_length_1676_cov_3.918569, whole genome shotgun sequence.
ACCESSION   DAOQFI010000529 DAOQFI010000000
VERSION     DAOQFI010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48310_length_1676_cov_3.918569"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLE93_08900"
     CDS             complement(<1..252)
                     /locus_tag="PLE93_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87208.1"
                     /translation="MLIGLIVLGFVLLTFTFGSGSGGVGGVLGTFFDPVTNAASRALK
                     PARDLINWFDETMDARGERDRLQSELEEARGKAVAGEVAL"
     gene            complement(462..1028)
                     /locus_tag="PLE93_08905"
     CDS             complement(462..1028)
                     /locus_tag="PLE93_08905"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNH87209.1"
                     /translation="MPACPVILASGSPQRRDLLERIGVEFEVRVSGVEEIVHGDPAEV
                     VLANALLKARSVAETAPGCLVIGCDTDVVLEDHIIGKPDDEVRAREYLAQLSGRKHRV
                     LSGLAIVGPEAEQVRTGVAESAVRFRELEKSEVDRYLASGEWRGRAGGYAIQGRGSSL
                     VAGVDGDIANVIGLPVGLLLDLAPELDL"
     gene            complement(1061..1624)
                     /locus_tag="PLE93_08910"
     CDS             complement(1061..1624)
                     /locus_tag="PLE93_08910"
                     /inference="COORDINATES: protein motif:HMM:NF037470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HNH87210.1"
                     /translation="MGVSEKVRRDDAIRLARRHYLNLERVDMQSLANELGIGRTTLYR
                     WVGDRDSLISEMLSEMVVEVIGTAIGEAEGEGLERTIDAMRRFMLTTADFAPLRHLVQ
                     NEPELGLRVMLAPGSGVSTAITGELTAEIERTRPEWSGEKASELADVLTQIGMAYEWG
                     NIAVRSEPEIDRAIRAMETLVRAADTA"
BASE COUNT          277 a          583 c          539 g          277 t
ORIGIN      
        1 gagtgcgacc tcgccggcga ccgccttgcc gcgggcttcc tcgagctcgc tctggagccg
       61 gtcccgctca ccgcgcgcgt ccatcgtctc gtcgaaccag ttgatcaggt cccgggccgg
      121 tttcagggct ctcgaggcgg cgttggtgac cgggtcgaag aaggtcccga gcacgcctcc
      181 cactccgccc gaaccactgc cgaaggtgaa tgtgagcagc acgaagccca gcacgatgag
      241 gccgatgagc acggcccggc gacgtctgac ttgtttgcgg tacatgtgag tggctggtgg
      301 tgattgagag gtcgcggaag agcgggcacc aatcgtgatc cggagtggaa tcgctccgaa
      361 cccgcgactt tctcagcttg gcaagagtgc cgcccggacg gcctggtgac tgcgggaagg
      421 attgcagcgg ccgaccaccc tacccggagg ccttcagcgg ttcataaatc cagttccgga
      481 gccaaatcca gtagcaggcc aaccggcagg ccgatcacgt tggcgatatc gccgtcgacc
      541 ccggcgacca gggaagatcc ccggccctgg atcgcgtagc cgccggcccg gccgcgccac
      601 tcgccgctcg ccaggtagcg gtcaacctcg ctcttctcca gttcacggaa gcggacggcc
      661 gattcggcca ccccggtccg gacctgttcc gcctcggggc cgacgatcgc cagcccgctc
      721 aggacccggt gtttccggcc ggaaagctgg gccaggtatt cgcgcgcccg gacctcgtca
      781 tccggtttgc cgatgatgtg gtcctcaagc accacgtcgg tgtcgcaacc gatcaccagg
      841 cagcccggcg ctgtttcggc caccgacctg gccttgagca gggcgttggc caggaccacc
      901 tccgccgggt cgccgtgcac gatctcttcg acgcccgaga cccgaacctc gaactcgacg
      961 ccgatccgtt cgagcaggtc gcggcgctgg ggtgaaccgg aagcgaggat taccgggcag
     1021 gctggcaccg aagcagaatc gcagatcgcg gtcccggcga tcaggccgtg tcggcggccc
     1081 ggaccagggt ctccatcgcc cggatcgcgc ggtcgatctc cggctcggag cggaccgcga
     1141 tgttgcccca ttcgtaggcc atgccgatct gggtgaggac gtcggccagc tcgcttgcct
     1201 tctcacccga ccattccggc cgggtccgtt cgatctcggc ggtcaactcg ccggtgattg
     1261 cggtcgaaac cccggaaccc ggagccagca tcacccgcag gcccagttcg ggttcgttct
     1321 ggaccaggtg tcgaagtggc gcgaagtccg cggtcgtcag catgaatcgg cgcatcgcgt
     1381 cgatcgtgcg ttcaaggccc tcgccctccg cctcgcctat cgcggtgccg atcacctcga
     1441 ccaccatttc ggagagcatc tccgaaatca gggagtcgcg atcccccacc caccggtaga
     1501 gggtcgtgcg gccaatcccc aactcgttgg ccagcgactg catgtcgacc cgctcgaggt
     1561 tgaggtagtg gcgccgcgcc aaccggatcg catcgtcacg acgaactttc tcggaaacac
     1621 ccacgcagca atcctagacc cgccttgagg cagtggttga gccgcggggt cgggcg
//
LOCUS       DAOQFI010000530         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48334_length_1676_cov_2.525601, whole genome shotgun sequence.
ACCESSION   DAOQFI010000530 DAOQFI010000000
VERSION     DAOQFI010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48334_length_1676_cov_2.525601"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(527..1189)
                     /gene="rlmB"
                     /locus_tag="PLE93_08915"
     CDS             complement(527..1189)
                     /gene="rlmB"
                     /locus_tag="PLE93_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNH87211.1"
                     /translation="MSDPEIIYGRNPVEEAKRGRRKVLRVWEAPETPETRLVELCGST
                     DHQGIVAEVEPFPYLSGSDLLAGEDALVVVLDQIQDPRNLGAICRSAEAAGATGVVIP
                     DRRAASVTAAAAKASAGAVEHLKIARVRNISDWIIEAKDAGFWIWGADGRADQAPWQV
                     DLTGKTALVLGTEEKGLRQRVADSCDGLIAIPQSGQVDSLNVSVAAAVLIFEAIRQRS
                     RT"
     gene            complement(1189..>1676)
                     /locus_tag="PLE93_08920"
     CDS             complement(1189..>1676)
                     /locus_tag="PLE93_08920"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNH87212.1"
                     /translation="HYRQPLAFGEGPLEQAAAGNERLRNFLRENPPAGAGEGVPGDEA
                     AAAGSRFRDALADDFNTPRAMREAFDLVRAVNQRPDDPALAGAGTVLAGMLELVGLES
                     LATGGEEGPGDEALELLEARNAARADKDWARADEIRDSLAELGWEVRDGAEGARLVPR
                     S"
BASE COUNT          267 a          560 c          563 g          286 t
ORIGIN      
        1 ggccctcctg gatcagatcg tcggaatcgc cgccgaccag gaagtaggaa gaagccttga
       61 gccggacaaa accgtggtac cgcctgatca gacgatcggc cgcatcggtc ttgccacgct
      121 tggcgaaggc gacgagggtc tcatcatcag gctcaagtcg agggttagat actgaaccgc
      181 gggatcgagc aggaacgtcg gaaaccgcat ggttgtggga aattgaggtt ggggtagcca
      241 ccgcacattc tcctttagaa gaaggcctgt ggcgccactt acctcccctg gacgcccttg
      301 ctgacagatg aatctcacac tcaagtcgag gttcaccctt tatctgtcgc ttagtttaca
      361 tatacacggg gttctgtcaa ctacctgcct ccaaatctgc aatccggctt gcagaagcga
      421 agtccaggga ggaaccgtga ggtgggtcgg atttccgtcg cctgtgcggg aaatccgacc
      481 cgcccgaagg ggcggaatgg tgatggggtg gcgcggggcc ggaggttcag gtccggctgc
      541 gctggcggat tgcttcgaag atcaggacgg cagcggcgac cgagacgttc agggagtcga
      601 cctggccgct ttgcgggatc gcgatcaggc cgtcgcacga atcggcgacc cgttgccgga
      661 ggcccttctc ctcggtgccg aggacgaggg cggtcttgcc ggtcaggtcc acctgccagg
      721 gggcctggtc tgcgcgtccg tcggctcccc agatccagaa gcccgcatcc ttggcttcga
      781 tgatccagtc actgatgttg cggacccggg cgatcttcag atgctcgacc gcgccggccg
      841 aagccttggc cgccgcggcg gtgaccgaag cggcccgtcg atcggggatc accaccccgg
      901 tcgcccccgc ggcttcggcc gaacggcaga tcgcaccgag gttgcggggg tcctggatct
      961 ggtcgaggac cacgaccagt gcgtcctccc cggccagcag gtcggacccg ctcaggtagg
     1021 ggaaaggctc gacctcggcc acgatcccct gatggtcggt cgaaccgcaa agctcgacca
     1081 gcctggtctc cggcgtttcg ggggcttccc agacccggag cacctttcgc cggccccgct
     1141 tggcctcctc gacgggattg cggccataga tgatctccgg gtcggacatc agctgcgggg
     1201 cacgagccgg gcgccttcgg ccccgtcacg gacctcccag ccgagttcgg ccagactgtc
     1261 ccggatctcg tcggcgcggg cccagtcctt gtcggccctg gccgcgtttc ttgcctcgag
     1321 cagctcgagc gcctcgtccc cgggtccctc ctccccgccc gtggcgaggg attccaggcc
     1381 gaccagctcc agcatcccgg ccagaacggt cccggcaccg gccagggcgg ggtcgtcggg
     1441 ccgctgattg acggcgcgaa ccaggtcgaa ggcctcgcgc atcgcccggg gagtgttgaa
     1501 atcatcggcc agcgcgtccc ggaaccggga accggccgcc gcggcctcgt cgccgggcac
     1561 gccctccccc gcccctgccg gcggattttc gcgcaggaag ttgcgcaggc gttcgttgcc
     1621 ggcagccgcc tgttcgagcg ggccctcgcc gaaggcgagt ggctggcggt agtgac
//
LOCUS       DAOQFI010000531         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48549_length_1672_cov_3.092146, whole genome shotgun sequence.
ACCESSION   DAOQFI010000531 DAOQFI010000000
VERSION     DAOQFI010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48549_length_1672_cov_3.092146"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLE93_08925"
     CDS             <1..331
                     /locus_tag="PLE93_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNH87213.1"
                     /translation="VRRPDRVLTEMKRVAPRLVVSLPNFAHWKMRATLALRGRMPVTD
                     ALPYQWYDTPNIHLCTIRDFEQLTGELDLKIDRRILIDATGRRTGGLARRAPNLLAER
                     VVYSLTA"
     gene            complement(357..908)
                     /locus_tag="PLE93_08930"
     CDS             complement(357..908)
                     /locus_tag="PLE93_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNH87214.1"
                     /translation="MPRIPYPTEDELEPEARERMANLPPLNLIRMFANAPTALGPLTD
                     LGQAILLQSELDPRLREVAILAVARACDSEYERLQHENICRAIGMNEEEIRAAADQNP
                     EALDEEARLIWTFADQIATSVRADEELTARVLEKLGRRQTTELVIACAFYSAMGRIIE
                     TTGVELEETLPTENIDPDEYTGD"
     gene            complement(938..1474)
                     /locus_tag="PLE93_08935"
     CDS             complement(938..1474)
                     /locus_tag="PLE93_08935"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH87215.1"
                     /translation="MSRIFGKVAQNGYVVKDIRSEMDHWVGLGVGPWFYMGRVEMDWF
                     RHRGEDSEPPEVSIALANSGDLQIELIQQRNDAPSMYREFIDAGHEGLQHMSYWSEDY
                     QSLYDKVLGLGFEIGHEGCIGGEQGRFAYLDTQTTPGTVIEISDVSGAKGMFFEHVRS
                     EAENWDGSDPIRELIPDP"
BASE COUNT          255 a          564 c          538 g          315 t
ORIGIN      
        1 ggtgcggcgg cccgaccggg tgctgaccga aatgaaacgg gtcgcgcccc ggctggtcgt
       61 ttcgctgccg aacttcgccc actggaaaat gcgcgccacg ctcgccctgc ggggacggat
      121 gccggttacc gacgccctcc cctaccagtg gtacgacacg ccgaacatcc acctctgcac
      181 gatccgcgac ttcgagcagc tgaccggcga gctcgacctg aaaatcgacc ggcggatcct
      241 gatcgacgcg accggtcgcc gcaccggagg cctcgccagg cgcgccccca acctgctggc
      301 cgaacgggtc gtctactccc tgactgcatg aacccggcag ttctctttgc tcgtgctcag
      361 tccccggtgt attcgtccgg gtcgatgttt tcggtgggga gggtctcttc gagttcgacg
      421 ccggtggttt cgatgatccg gcccatcgcc gagtaaaagg cgcaggcgat caccagctct
      481 gtggtctggc gccggccgag cttctcgagc acccgggcgg tcagttcctc gtcggcccgc
      541 acactggtgg cgatctggtc ggcgaaggtc cagatcaacc gcgcttcctc gtccagtgcc
      601 tccgggttct ggtcggcggc cgcgcggatc tcctcctcgt tcatgccgat cgcgcggcag
      661 atgttctcgt gctgaaggcg ctcgtactcg gagtcgcagg cgcgggccac cgccagaatc
      721 gccacttccc ggagccgcgg gtcgagttcg gactggagca ggatcgcctg accgagatcg
      781 gtcagcggcc cgagtgcggt cggggcgttg gcgaacatgc ggatcaggtt gagcggcggc
      841 aggttcgcca tccgttcacg cgcttcgggt tcgagctcgt cctcggtcgg gtagggaatg
      901 cggggcatgg tcggcctttc tcggggtgtt cggggtttca ggggtcgggg atcagctcgc
      961 ggatcgggtc cgagccatcc cagttctcgg cctcggagcg gacatgctcg aagaacatcc
     1021 ccttggctcc gctcacatcc gagatctcga tcacggtccc cggggtcgtc tgtgtgtcca
     1081 ggtaggcgaa gcggccctgc tcgccgccga tgcagccctc gtgcccgatc tcgaagccca
     1141 acccgaggac cttgtcgtag agggactggt aatcctcgct ccagtaggac atgtgctgga
     1201 gaccctcgtg gccggcgtcg atgaattccc tgtacataga aggggcgtcg tttcgctgct
     1261 ggatcagctc gatctgcagg tcacccgagt tggccagggc gatgctcacc tcgggcggtt
     1321 ccgaatcttc gccgcgatgg cgaaaccagt ccatctcgac ccggcccatg taaaaccagg
     1381 ggccgacgcc gagcccgacc cagtgatcca tctcggacct gatgtccttg acgacatacc
     1441 cgttctgggc gacctttccg aaaatccgac tcactccggc tcctctcctc gaccgctgac
     1501 aacccggagc ttatgaccct cgatcgggag tcgggccggc cagccctcag gcgacggcgc
     1561 gaagctggtc gaggcggtga tcggcccgct ggaccgaggt gacttcctgc caggtcaggc
     1621 gcaggccgtc gacttcactt gactcatatc ccgccggagc cccggccccg tt
//
LOCUS       DAOQFI010000532         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48556_length_1672_cov_2.598639, whole genome shotgun sequence.
ACCESSION   DAOQFI010000532 DAOQFI010000000
VERSION     DAOQFI010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48556_length_1672_cov_2.598639"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLE93_08940"
     CDS             <1..361
                     /locus_tag="PLE93_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNH87216.1"
                     /translation="DEPQTDRVYEQWATTGSKWKIPLLTQVVETIPFSGKRSVPYGTG
                     TVRDFMHAKVTVADDTVFFGSFNLSRSGEANAENMVEIEDLGIAGKMAKFIDETRALY
                     PRSTVPDRRGPRKGERT"
     gene            361..1341
                     /locus_tag="PLE93_08945"
     CDS             361..1341
                     /locus_tag="PLE93_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH87217.1"
                     /translation="MLPLRGKAAEALDGAMDFMVVPGFSRIGYEVRRRMFDWQMPDLA
                     GRSVMVTGATSGLGEAAAIDLARCGAIVHIVARNPDKAEETRVEIQSAAPGAPVSVHL
                     CDLSLTTDIRRFTDAFLKSGDRLDVLINNAGVMPAEREYTDEGFELTFATNLIGPYLL
                     TELLLPRLREGDNPRVITMSSGGMYSSGFSFRDPQLAEKDYKPTSFYAHTKRGEVMIT
                     DAWQRREPEGGVTFCSMHPGWAVTPGMSAALPGFHKVTGPILRDSHEGVDTAIWLAGA
                     TPAEAPGGTFYEDRRPRTKQRMPGTAGSSEDGERLLELLAGITSGSDAEG"
     gene            1384..>1672
                     /locus_tag="PLE93_08950"
     CDS             1384..>1672
                     /locus_tag="PLE93_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87218.1"
                     /translation="MWLAALGLSFFALLLPGHSAAARAAGECTLIPGGTIGDPEPEPV
                     PDRAGSPTDRGPGAHGANPTLKGLPTRVDLRSPSMAQNRNHYFALKNGRLFV"
BASE COUNT          294 a          569 c          540 g          269 t
ORIGIN      
        1 cgacgaacca cagacggacc gcgtctatga acagtgggcg accaccggct ctaagtggaa
       61 gattccgctg ctcacccagg tggtggaaac gattccgttc agcggcaagc ggtcggttcc
      121 ctacggcacc ggcaccgtcc gcgacttcat gcacgccaag gtcacggtcg ccgacgacac
      181 cgtcttcttc ggttccttca acctttcccg ctctggggaa gccaacgccg agaacatggt
      241 ggaaatcgag gatctgggta tcgccgggaa gatggcgaaa ttcatcgacg agaccagggc
      301 gctctacccg cgcagcaccg tgccggaccg gcgcggcccg cgcaagggcg agcgaacctg
      361 atgctcccgc tccggggcaa ggccgccgag gccctcgacg gagccatgga cttcatggtg
      421 gtgccgggct tcagccggat cggctacgaa gtccgccgtc gcatgttcga ctggcagatg
      481 ccggacctgg ccggccgcag cgtgatggtt accggggcaa cctccggcct cggtgaagcc
      541 gccgcgatcg acctcgcccg ttgcggggcg atcgtccaca tcgtggctcg caatccggac
      601 aaggccgagg aaacccgggt ggaaatccag tccgccgctc ccggtgcccc ggtcagcgtt
      661 catctctgcg atctctcgct gaccacggac attcggcgtt tcaccgacgc gttcctcaaa
      721 tcaggcgacc ggcttgacgt gctgatcaac aacgccgggg tgatgccggc ggagcgtgaa
      781 tacaccgatg agggattcga gctgactttc gccaccaacc tgattggccc gtacctgctc
      841 accgagcttc tgctgccccg gctgagggaa ggcgacaacc cgcgggtgat cacgatgagt
      901 tccggcggca tgtactcgag cgggttcagc tttcgcgacc cgcaactggc ggagaaggac
      961 tacaagccaa ccagcttcta tgcccacacc aaacggggcg aggtcatgat caccgacgcc
     1021 tggcagcggc gcgaacccga aggcggggtg accttctgct cgatgcatcc gggctgggcg
     1081 gtcacaccgg gcatgagcgc cgcactgccc ggcttccaca aggtgaccgg tccgatcctg
     1141 cgggattcgc atgaaggtgt cgacacggca atctggctcg ccggcgcgac tccggcagag
     1201 gcgccgggcg gaaccttcta cgaggatcgc cggccccgga ccaagcagcg aatgccgggg
     1261 actgccggct cctccgaaga tggcgaacgg ctgcttgagc tactggctgg tataaccagt
     1321 ggctccgatg ccgaggggtg atcattgaaa ccgggccggt tcccgcccgc gcagctgtcg
     1381 attttgtggc tggcggccct ggggctgtcc ttcttcgccc tgctgctgcc cggccattca
     1441 gcggccgcca gggcggcggg ggaatgcacg ctcatcccgg gcggcacgat cggcgacccc
     1501 gagcctgagc cggtcccgga ccgggctggt tctccgaccg accggggtcc gggcgcccac
     1561 ggcgccaacc cgaccttgaa aggcctgccg acccgggtcg acctgcgctc gccgtcgatg
     1621 gcccagaacc gcaatcacta cttcgccctc aaaaacggcc ggctcttcgt ca
//
LOCUS       DAOQFI010000533         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48597_length_1671_cov_3.244431, whole genome shotgun sequence.
ACCESSION   DAOQFI010000533 DAOQFI010000000
VERSION     DAOQFI010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48597_length_1671_cov_3.244431"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLE93_08955"
     CDS             <1..808
                     /locus_tag="PLE93_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH87219.1"
                     /translation="RDVKPGNLMIVGGPVGGGSELTVKLTDFGIARAAEQTRITQVGS
                     VVGTAAYLAPEQVRGEEATPSTDVYALGVVLYQFLTGRLPYEGSSLAELAVRQQNEKP
                     LPPRTYNADVPETVSAAVMRALEGDKAYRFNTAAALSDGLQRGLRGEDVTLQMDEEPV
                     TPAEFPQAETATRPLERTARTAHRPAPPPPRYTLPQTAPRPRQKRSFIRSFFRFVLGI
                     LALVLIAAVVAGSVILLTDRAASVKIKEWTGNSVEKISNELKQQIRDNTQ"
     gene            complement(809..1534)
                     /locus_tag="PLE93_08960"
     CDS             complement(809..1534)
                     /locus_tag="PLE93_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87220.1"
                     /translation="MRAELLKLRSMPTPRWTLAILLAFFVGGIVASVIWGVGTDSAVL
                     DLAVGLPGQIAALVVGSWMAGVEFGQNTLRRALSADPRRLRLVLSKLAVLVLAVVAVT
                     VILIVLGTLIFSLAGSGHATSIEIGTAGKILGAWLVADISWAVTAFSLTLLTRSMAGG
                     LVLSFVFFFVLDGFISLIPKVGDYTIGIALSDIDIAIRGTTDDFFGQTTTNDTGIAVI
                     VLVGWLAVFIAAGIVRTLKTEVK"
BASE COUNT          323 a          567 c          543 g          238 t
ORIGIN      
        1 ccgcgacgtc aagccgggaa acctgatgat cgtcggcggt ccggtcgggg gcggctccga
       61 gctgaccgtg aagctgaccg acttcgggat cgccagggcg gccgagcaga cccggatcac
      121 ccaggtcggc tccgtggtcg ggaccgccgc ctacctggcc cccgaacagg ttcggggtga
      181 agaggcgaca ccgtcgaccg acgtttacgc cctgggtgtc gtgctctacc agttcttgac
      241 cggtcgcctg ccatacgagg gttcctcgct ggccgagctt gcggtgcgcc agcagaacga
      301 gaagccgctg ccgccgagga cctacaacgc cgacgtgccg gaaaccgtaa gcgccgcggt
      361 gatgagggcg ctcgaaggtg acaaggccta ccgcttcaac accgccgcgg ctctttccga
      421 cgggctccag cgagggttgc ggggcgagga cgtgactctc cagatggacg aggaaccggt
      481 aactccggcc gaattcccgc aggccgagac cgcgacccgg ccgctggaac gcacggcccg
      541 caccgctcac cggccggccc cgccgccgcc ccgctacacc ctcccccaga ccgctccccg
      601 gccccgccag aagcgttcgt tcattcgctc tttcttccgc ttcgtgctgg gcatcctcgc
      661 cctggtcctg atcgccgcgg tggtcgcggg ttcagtcatc ctgctgaccg accgggcagc
      721 ctcggtcaag atcaaggaat ggacggggaa ttcggtcgag aagatctcga acgagctgaa
      781 gcagcagatc cgcgacaaca cccagtagtc atttcacttc ggtcttgagg gtgcggacga
      841 ttcccgccgc gatgaagacc gccagccagc cgaccaggac gattaccgcg atcccggtgt
      901 cattggtcgt ggtttgaccg aagaagtcat cggtggtgcc gcggatcgcg atgtcgatgt
      961 cagacagggc gatgccgatc gtgtagtccc cgaccttcgg aatcagtgaa atgaagccgt
     1021 cgaggacgaa gaagaagacg aaggaaagga ccaggccccc ggccatcgac cgggtgagaa
     1081 gggtgagcga gaaggcggtc accgcccagg aaatgtcggc gaccagccag gcgccgagaa
     1141 tcttccccgc ggtgccgatc tcgatcgagg tggcatgccc cgagccggcc agactgaaaa
     1201 tcagggttcc gaggacgatc aggattaccg tcaccgcgac cacggccagg acgagcacgg
     1261 ccagcttcga aagcaccagg cgaagccgcc gcggatcagc cgagagcgcc cgccgaagcg
     1321 tgttctgccc gaactccacc ccggccatcc aggagccgac gaccagcgcc gcgatctggc
     1381 ccggaagccc aaccgcaagg tcgagtacgg cactgtcggt ccccacgccc cagatcaccg
     1441 aagcaacgat gccgccgacg aagaaggcga gcaggatcgc cagcgtccag cgcggtgtcg
     1501 gcatcgaacg cagcttgagc agctcagctc tcatcgccgg ctccatcggt cagcgagagg
     1561 aagaccctct cgaggtcggg acgctcgcgc atcagcccgc gcagccagat gccggattcg
     1621 gcgaggatgc gggtgatctc cgagtcgtcc tcgatctcgc cccgggcgac g
//
LOCUS       DAOQFI010000534         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48657_length_1670_cov_3.536223, whole genome shotgun sequence.
ACCESSION   DAOQFI010000534 DAOQFI010000000
VERSION     DAOQFI010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48657_length_1670_cov_3.536223"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLE93_08965"
     CDS             <1..581
                     /locus_tag="PLE93_08965"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNH87221.1"
                     /translation="VMLQELGLIFPEEEVKVAWEILKALKLREKGPVLIACPTCGRLQ
                     FDMDAVVAEVEARLETYDEPIEVAVLGCAVNGIGEAKGADFGIAGAKNEGVVFAKGKA
                     IKKVPTSQLVETLFEQINLSLERDGKVEFDEKESAEGEAWVKKIEDENAGDLTPEKIA
                     KMEHEASQKAENDKLLVDEEVSPTAGRRFTRA"
     gene            674..1549
                     /locus_tag="PLE93_08970"
     CDS             674..1549
                     /locus_tag="PLE93_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH87222.1"
                     /translation="MPEGPSKTAEYVATFRAVESARPEGERLFSDPYAASFLDGKLRL
                     GAALARNGMGQRLVCRYIDRNWPGSRPSAVIRTRLIDDAVTAAIEGGAEQLAILGAGY
                     DTRGLRLPAAREIPVFELDQPPTQERKKQRLRARDGDLPSNVTYVPFDLLDEGVGAPL
                     ERAGFRTGLKSVYIWEGVLSYLTPEAVDQTLAWITASGAPGSRLVYTYVDVALLKQGG
                     EDEPPWSSQVAKVGEPFRYGLDPAKMDAFLGDRGLKPVWDQSTLEAIAQHAVPGGRNA
                     PGFYRVAEAEVLPDR"
BASE COUNT          335 a          480 c          583 g          272 t
ORIGIN      
        1 aagtcatgct tcaagagttg ggacttattt tccccgaaga agaagtcaag gtcgcctggg
       61 aaatcctcaa ggcactcaag ctgcgagaga agggcccggt gctgatcgcc tgcccgacct
      121 gcggccggct ccagttcgac atggacgccg tggtcgccga agtcgaggca agactcgaga
      181 cctacgacga gccaatcgag gtagccgtgc tcggctgcgc ggtcaacgga atcggcgagg
      241 ccaagggtgc cgatttcgga attgccggag ccaagaacga gggtgtcgtc ttcgccaagg
      301 gcaaggcgat caaaaaggtg ccgacttcac agctcgtcga aacgctcttc gagcagatca
      361 atctttcgct tgaacgcgac ggcaaggtcg agttcgacga gaaggaatcg gccgaaggcg
      421 aagcctgggt caagaagatc gaggatgaga acgccggcga tctgaccccg gagaagatcg
      481 cgaagatgga gcatgaggct tcgcagaagg cggagaacga caagcttctg gttgacgaag
      541 aggtatcacc gaccgccggt cgtcgtttca cccgagcctg atgctggggg cgggggctgg
      601 cagagattgc ctcctcggcc cgaaacgtcg tcggcaccct ctgccagtcc ccgcccgacc
      661 ggaattgcgg tagatgccgg agggtccttc caagactgct gaatacgtcg ccacgtttcg
      721 cgcggtggaa tcggcgcggc ccgaagggga gaggcttttc agtgacccct acgcagcgag
      781 ctttcttgac gggaagttga ggttgggggc ggctttggcc cggaacggga tggggcagag
      841 gctggtttgc cgctatatcg accggaactg gcctggttcg cggccttcgg cggtgatccg
      901 tacccggctg atcgacgatg cggtcaccgc ggcgatcgag ggaggggcgg agcagctggc
      961 gattctcggg gccggatacg acaccagggg tttgcggctg ccggcggcca gggagatacc
     1021 ggtcttcgag ctcgaccaac ccccgacgca ggagcgcaag aagcagagac tgagagcacg
     1081 ggacggcgat cttccgtcga acgtgaccta cgtgcctttt gatctgctcg acgagggggt
     1141 cggcgcacca ctggaacggg cagggttcag gaccggcctg aagtcggtct acatctggga
     1201 gggggtactg agctacctga caccggaagc ggtggaccag accctggcct ggatcacggc
     1261 ctcgggagca cccggaagca ggctcgtcta cacctatgtc gacgttgccc tcctcaagca
     1321 gggaggcgag gacgagcccc cgtggagcag tcaggtggca aaggtggggg agccgtttcg
     1381 ctacggcctg gatccggcga agatggatgc ctttctgggc gaccgaggcc tgaaaccggt
     1441 ctgggaccaa tcgaccctgg aggcgatcgc acagcacgcg gtgccgggtg gccggaacgc
     1501 cccgggcttc taccgggtcg cagaggccga agtccttccc gacagatagg ctccgacctc
     1561 gatgacgagg ctctcgcgca cacttctgcc taccctgaag gatccgcccg ccgacgccga
     1621 agcggtgagc cacaagctgc tggttcgggc gggaatgatc cggcaggtcg
//
LOCUS       DAOQFI010000535         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_48957_length_1665_cov_3.733540, whole genome shotgun sequence.
ACCESSION   DAOQFI010000535 DAOQFI010000000
VERSION     DAOQFI010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48957_length_1665_cov_3.733540"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLE93_08975"
     CDS             complement(<1..606)
                     /locus_tag="PLE93_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87223.1"
                     /translation="MRGAPTRRARRQNITPSGLMLVVFGFMALTFIVLLPAKAGAAYL
                     DGAGTASLQNSPVEPEAPAPDSGASGSAVSSGGGLGVPGLPEISRVICLRQCVSSSRA
                     TRGAIIAIRGENLDRVIRVIFRGKRGWLRAKYRTRSKAAVRVTVPRQAIRGYLAVVDS
                     GGHRSRSPNELRIFPISAIPIEVFPVRGPVSFGSSGSRFGAG"
     gene            844..>1665
                     /locus_tag="PLE93_08980"
     CDS             844..>1665
                     /locus_tag="PLE93_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNH87224.1"
                     /translation="MTAIRQKNVTAAQWNANGSAVESTDLTKEENVVFGSNVFSIKTQ
                     KERLPADVFDRLQGTLERGEALDVDLADAVADAMKDWALENGATHYTHVFQPLTGLTA
                     EKHDSFFRPTDEGGSIAKFKGSELIQGEPDASSFPTGGIRATFEARGYTAWDPTSPAF
                     LLDNPNGTLLCIPTAFASWTGEALDAKIPLLRSMDALSRSAVKALKLLGDEETSRVVT
                     TVGPEQEYFLIDEQYFFERPDLYTTGRTLFGAKPPKGHELDEHYFGSIPERVLAYM"
BASE COUNT          325 a          563 c          498 g          279 t
ORIGIN      
        1 gccggccccg aagcgggagc cggaactgcc gaagctgacc gggccgcgaa ccgggaagac
       61 ttcgatcgga atcgcggaga tcgggaagat cctcaattca ttcggcgagc ggctgcggtg
      121 acctcccgag tcaaccacgg cgaggtagcc ccggatcgcc tgcctgggaa cggtcactcg
      181 aaccgcggcc ttgcttcggg tccgatactt cgcacgcagc cagccccgct tgccgcggaa
      241 gatcacgcgg atgacccggt cgaggttctc gccccgaatc gcgatgatcg cgccccgggt
      301 ggccctgctg cttgaaacgc attggcgcag gcagatcacc cggctgatct ccgggagtcc
      361 gggaacgccc aggccgccgc ccgatgaaac ggccgaaccc gacgcgccgg aatcaggggc
      421 cggtgcctcc ggctcaaccg gcgaattctg aagagatgcg gtcccggctc cgtccaggta
      481 ggcggccccg gctttggccg gcagcagaac aatgaaagtg agtgccatga acccgaagac
      541 gaccagcatc agaccgctgg gggtgatgtt ctgtctcctg gccctgcgcg ttggagctcc
      601 tcgcatcgtg gacaccataa catgagttct agacgatgat tccgtgagct gctgcacaca
      661 aacttgcaga tcggactttc aggtaccccg tttcatcagt gtcggagcca agcctgtccc
      721 acgggagggc ggccctgaac taaccttgtc ctgaggtaca agcgaccgca tggccgctcg
      781 gggatagcct cgacttcgct ttttccctcg aaatcaatct tgccaccagg aggaactctc
      841 caaatgaccg cgatccgcca gaagaacgta accgctgccc agtggaacgc caacggttcg
      901 gccgttgagt ccacggacct gaccaaggaa gaaaacgtcg tattcggcag caacgtcttc
      961 tcgatcaaga cccagaagga gcggctcccc gccgatgtct tcgaccggct tcagggcacg
     1021 ctcgaaaggg gcgaggccct tgacgtcgat ctggccgacg cggtcgccga cgcgatgaag
     1081 gactgggcgc tcgaaaacgg cgccacccac tacacgcatg tcttccagcc gctgacaggt
     1141 ctgaccgcgg aaaagcacga ttcgttcttc cgcccgaccg atgaaggcgg ctcgatagcc
     1201 aagttcaagg gttcggaact gatccagggc gaaccggacg cctcctcctt ccccaccggc
     1261 ggcatccgcg ccaccttcga agcccgcggc tacaccgcct gggatccgac cagcccggcc
     1321 ttcctgctcg acaacccgaa cggaacgctg ctctgcatcc cgaccgcttt cgcttcctgg
     1381 accggcgaag ccctggacgc gaagatcccg ctgcttcgtt cgatggatgc cctttcgcgt
     1441 tcggcggtca aggcgctgaa gctgctcggt gatgaagaga cttcgcgcgt ggtcaccacg
     1501 gtcggcccgg agcaggagta cttcctgatc gacgagcagt acttctttga gcgccccgac
     1561 ctctacacca cgggccgcac gctgttcggt gccaagccac cgaagggcca cgagctcgac
     1621 gagcactact tcggttcgat ccccgagcgc gtcctcgcct acatg
//
LOCUS       DAOQFI010000536         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_49393_length_1658_cov_2.560200, whole genome shotgun sequence.
ACCESSION   DAOQFI010000536 DAOQFI010000000
VERSION     DAOQFI010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49393_length_1658_cov_2.560200"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLE93_08985"
     CDS             complement(<1..567)
                     /locus_tag="PLE93_08985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH87225.1"
                     /translation="MSRDSDTNYAGAIYGTIVSMAVIATSAKDPSLGPVLIAFWAAVT
                     ALIFWLAHVYADIVAEGYARFRDAGQVGPFVRDDLRACLPSWPAERIETLYNRIDVDA
                     VQADLLDRAAARAFLQLPQNAWVVGNVGRLHPDKDQASLIRGFARALPRLPPGSLLAI
                     MGSGRLEPMLRGLVADLQLSGQVRFLGQV"
     gene            679..>1658
                     /locus_tag="PLE93_08990"
     CDS             679..>1658
                     /locus_tag="PLE93_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87226.1"
                     /translation="MNIQSFVRRILPALIILVGGFTATGTQAIAAGKARPVKVSIAAI
                     STANQQQLIKQKKLAVKVKSTGKVSARLIATSGGEGGYFKATTVKFSRKGSKTVKLAL
                     TSSGKTALSKCGAKTVTTVASYRRKVGGKSKQAKSSKKKTLAKWAANENCIEYASVEL
                     GDDPEHCDFMDTTVCLQPWPNDYYMKDDASTPTGKRLDIAPGATPENTDGTHIAVTDI
                     NRGDGFSPGNLIVLKVPGLDTPAAFANNDFVTLSDLHAYDDPAQRVMLIDAATGERQP
                     IWTELDANPTSTDPGEDDPGGINTNPSNTTPVNLIVRPARNLEWGHRYIVA"
BASE COUNT          369 a          547 c          498 g          244 t
ORIGIN      
        1 cacctggcca agaaagcgca cctgcccgga cagttgcaag tcggcgacca gacccctcag
       61 catcggctcc agcctaccgc tgcccatgat tgccagcaga ctgcccggcg gcaaacgagg
      121 cagcgcgcga gcaaaaccgc gaatcaaact ggcctgatcc ttgtccgggt gtaatcgccc
      181 gacgttccct accacccaag cgttctgagg cagctgcaga aaggcacgtg ctgccgcgcg
      241 gtccagaaga tccgcctgta cagcatcgac gtcgatacgg ttgtacaggg tttcgattcg
      301 ttcagcgggc caggaaggca ggcaagctcg caggtcgtcg cgcacgaatg ggcccacctg
      361 gccggcatca cggaaccggg cgtaaccctc ggccacgatg tcggcgtaga catgggccag
      421 ccagaagatc agggcggtga cggcggccca gaaggcgatc agcaccggac ccagcgaagg
      481 gtccttggcc gaggtcgcaa taaccgccat cgagacgatc gtgccgtaga tcgcccccgc
      541 gtagttggtg tccgaatcgc ggctcacagc gcagaaccat agtgacccgg gcgccgtggg
      601 ctagaatgcc cccgcccttg gagagtgggt gttccggcgg aaggaatccg cggatcaaaa
      661 agcaatcagg gagacctttt gaacattcag agtttcgtcc gccggattct tccggcgctg
      721 atcatcctcg tcggcgggtt cactgccacc ggcacccagg ccattgctgc cggcaaggcc
      781 agaccggtca aggtgagcat cgcggcaatc agcactgcca atcagcagca gctgatcaag
      841 cagaagaagc tggccgtgaa ggtcaaatcg acgggcaagg tctctgcccg gctgatcgcg
      901 acgagtggtg gcgagggcgg ctacttcaag gccaccacgg tgaagttctc ccgcaagggc
      961 agcaagaccg tgaaactcgc cctcacctca tccggaaaga ccgcgctttc caaatgtgga
     1021 gcgaagaccg tgacgacggt cgccagctac cgccgcaagg tcggcggaaa gtcgaagcag
     1081 gcgaagtcct cgaagaagaa gacactcgcc aagtgggcgg ccaacgagaa ctgcatcgaa
     1141 tacgcaagcg tcgagctcgg tgacgatccg gagcactgcg acttcatgga cacaaccgtc
     1201 tgcctgcagc cctggccgaa cgactactac atgaaggatg acgcctcgac tcccaccggc
     1261 aagcggctgg acatcgctcc cggagcgact cccgagaaca ccgacgggac ccatattgcg
     1321 gtcaccgaca tcaatcgcgg tgacggcttc agcccgggca acctgatcgt gctgaaggtg
     1381 cccggactgg acacgccggc tgccttcgcc aacaacgatt tcgtcactct gagtgacctt
     1441 catgcctacg acgaccccgc ccagcgggtc atgctgatcg atgccgccac cggcgagcgc
     1501 cagccgatct ggaccgaact cgacgcgaac ccgacctcga ccgatccggg cgaggacgac
     1561 ccgggcggaa tcaacacgaa tccttccaac accacaccgg tcaacctgat cgtccgaccg
     1621 gcccggaacc tcgagtgggg ccaccgttac atcgtcgc
//
LOCUS       DAOQFI010000537         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_49498_length_1656_cov_3.672705, whole genome shotgun sequence.
ACCESSION   DAOQFI010000537 DAOQFI010000000
VERSION     DAOQFI010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49498_length_1656_cov_3.672705"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1567)
                     /locus_tag="PLE93_08995"
     CDS             complement(98..1567)
                     /locus_tag="PLE93_08995"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH87227.1"
                     /translation="MTEPDEEVDVVIVGAGISGISMAHQIGLDHPGLSYAVLENRTRI
                     GGTWDLFRYPGIRSDSGMGTFAFSFRPWPGDRHFGNGEQIQQYLEDTAAEYGIDQHIR
                     FGRKVVGADWDSAEARWTVTVAHSAENGSSRGSTSRIRCRFLFGCTGYYRYDKGFSPV
                     FEGEEDFGGELFHAQHWPEGLDPRGRRFVVIGSGATAITVVPALVEAGADVTLLQRSP
                     TYVLSIPTTGGIGGWIRSRFSPRRAAFLLRWFGILMEIVNFNLARRYPNFFRRLFISG
                     ARKQLPPDIERRHLNPRYDPWTQRVCFAPDGDYFAALSSESARIETGNIERISSDRIH
                     LREGGEIEADVIVKATGLELLLFGDIAMTVDGEPVNPAERLVYKGAMLSGIPNFVFVV
                     GYTNASWTLKSELIARFASRFIAEVDRAGAASGTPVAPEIPMKTVPLLNLDSGYILRA
                     RDRLPLAGDTSPWVLHMNYFSDRRELLGADMHDGALRYS"
BASE COUNT          322 a          507 c          550 g          277 t
ORIGIN      
        1 catgcggcga ctcgctccca tgccttgaag ttacggaacc ccaatcctat ttcctgcgaa
       61 atattcgtga atttgcaggg aaaaagctgc gaacgactca ggagtacctg agtgcgccgt
      121 catgcatgtc ggcaccgaga agttcacgcc ggtccgagaa gtagttcatg tgaagcaccc
      181 agggggaggt gtctccggcc agcggaaggc gatcccgggc ccgcagtatg taaccggaat
      241 cgaggttgag caaaggcacg gtcttcatcg ggatctccgg agcgaccggc gtgccgcttg
      301 cggcgcctgc gcgatcgacc tcggcgatga aacggctggc gaaccgggcg atcagctccg
      361 acttcagggt ccaggaggcg ttcgtgtaac cgaccacgaa gacgaaattg ggaatcccgg
      421 agagcatcgc tcccttgtag accagccgtt cggcgggatt gacgggttcg ccgtcgacag
      481 tcatggcgat atcgccgaac agcaggagtt ccagcccggt cgccttgacg atcacgtcgg
      541 cctcgatctc gccgccctcg cggaggtgga tccggtcgga gctgatccgc tcgatgttcc
      601 cggtttcgat ccgggcactc tcgctggaaa gggcggcgaa gtaatcgccg tcgggggcga
      661 aacagactcg ctgggtccag gggtcgtagc gggggttgag atggcgccgc tcgatgtcgg
      721 gcggtagctg cttgcgggcg ccgctgatga aaagccggcg gaagaagttc ggataacggc
      781 gggcgagatt gaagttgacg atctccatca ggatcccgaa ccagcggagc aggaaggccg
      841 cccgccgcgg cgagaaacgg ctacggatcc agcccccgat gccgccggtg gtcgggatcg
      901 aaagcacgta agtgggagag cgctggagca gggtcacatc ggctccggct tcgaccagag
      961 ccggcacgac ggtgatcgcg gtcgcgccac ttccgatcac cacgaaccgg cggccccggg
     1021 ggtcgagtcc ctcgggccag tgctgggcgt ggaagagctc tccgccaaag tcctcttcgc
     1081 cctcgaagac cggcgagaaa cccttgtcgt accggtaata gccggtgcag ccaaacagga
     1141 accggcagcg gatccgggag gtggatcccc gggatgaacc attttcggca ctgtgggcga
     1201 ccgtcaccgt ccagcgcgct tcggcgctgt cccagtcggc gccgaccacc ttccggccga
     1261 agcgaatgtg ctggtcgatt ccgtattcgg ccgccgtgtc ctcgaggtac tgctggatct
     1321 gctccccgtt gccgaagtgg cggtcaccgg gccagggacg aaacgaaaag gcgaaggtgc
     1381 ccatcccgga gtcggaacgg atgcccgggt agcgaaagag atcccaggtc ccgccgatcc
     1441 gcgtgcggtt ctcgagcacg gcgtagctca ggccggggtg gtcgagaccg atctgatggg
     1501 ccatcgagat gccggaaatc ccggcaccca cgatgaccac atcgacttct tcgtcaggct
     1561 cagtcactgt ccagagagta cttcccggcc agtgaaccgg ccagtcctac aggccgtagg
     1621 agcgaccaat cacttccagc atcacctcgt cggttc
//
LOCUS       DAOQFI010000538         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_49501_length_1656_cov_3.334791, whole genome shotgun sequence.
ACCESSION   DAOQFI010000538 DAOQFI010000000
VERSION     DAOQFI010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49501_length_1656_cov_3.334791"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLE93_09000"
     CDS             complement(<1..162)
                     /locus_tag="PLE93_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNH87228.1"
                     /translation="MRVLVIDNYDSFTYNLVQYLGELGAEVETVRNDVRTVDELLAEG
                     HDRLVVSPGP"
     gene            complement(162..>1656)
                     /gene="trpE"
                     /locus_tag="PLE93_09005"
     CDS             complement(162..>1656)
                     /gene="trpE"
                     /locus_tag="PLE93_09005"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNH87229.1"
                     /translation="TPDLETARALVPESNVVPVALRYTDDVETPVSALLKLRQGGPCF
                     LLESAERGQVGRYSFVGVGPHRVIRWVAGELKEFPGEAMSGEGAPIATFPAADPYDGV
                     KEYLSRYRVAETPGMPPFAGGAVGLFGYDLVRAIEPLGDPNPDPLGLPDLALMITDVL
                     LAFDHLHNELTIISCAWADEHENFDQAWEAAAERIGAVRESLRGPVPDAGETGAVEAP
                     EFTSNVTRDEFEATVARIVEYIHAGDAFQVVPSQRFSADQKAEALSIYRGLRSINPSP
                     YMYFFEFGDFQIAGASPEPLLKVNGDRVEIRPIAGTSPRGEDEEEDRRLAEKMISDPK
                     ERSEHVMLVDLARNDIGREAGYGTVEVEELMVVETYSHVMHIVSRVSGRLPEGRGALD
                     VLRSALPAGTLSGAPKVRAMQIIDEMEKVKRCSYGGAVGYLSWNGDLDTAIHIRTALI
                     KDGQVHIQAGGGTVADAKPEYEYEESVNKAKAMFRAVEIACENPDWA"
BASE COUNT          270 a          551 c          542 g          293 t
ORIGIN      
        1 gggacccggt gatacaacca gccggtcgtg gccttcggcc agcagctcgt cgaccgtccg
       61 gacatcattg cgaaccgttt ccacttccgc cccgagctca ccgaggtact ggaccaggtt
      121 gtaggtgaag gagtcgtagt tgtcgattac cagtactcgc atcaggccca gtccgggttt
      181 tcgcaggcga tctcgaccgc gcggaacatc gccttcgcct tgttgaccga ctcttcgtac
      241 tcgtactccg gcttcgcgtc ggcgaccgtg ccgcccccgg cctgaatgtg aacctggccg
      301 tccttgatca gggcggtgcg aatgtggatc gcggtgtcga ggtcaccgtt ccaggagagg
      361 tagccgacgg cgccgccgta ggagcagcgt ttgaccttct ccatctcgtc gatgatctgc
      421 atcgcccgga ccttcggcgc gccggaaaga gtgccggcgg ggagggcgga acggagcacg
      481 tcgagggcgc cgcggccctc cggcagcctg cccgagaccc ggctgacgat gtgcatcaca
      541 tgcgaatagg tctcgaccac catcagctct tccacctcga ccgtgccgta cccggcttcg
      601 cggccgatgt cgttgcgggc gaggtcaacc agcatcacgt gctcggatcg ctccttcggg
      661 tcggagatca tcttctcggc gaggcggcga tcctcttcct cgtcctcccc gcgcggcgag
      721 gtgccggcga taggccggat ctcgacgcgg tctccgttca ccttgagcaa cggctcgggt
      781 gaggctccgg cgatctggaa atcaccgaac tcgaagaagt acatgtacgg cgaggggttg
      841 atcgatcgaa gcccccggta tatcgagagg gcttcggcct tctgatcggc gctgaacctc
      901 tgtgagggca ccacctggaa ggcgtcgccg gcgtggatgt actcgacgat ccgggcgacc
      961 gttgcctcga actcgtcccg cgtcacgttg gaagtgaact cgggagcttc aaccgcgccg
     1021 gtctcgcccg cgtcgggcac cggtccgcgc aggctttccc tgaccgcccc gatccgttcg
     1081 gcggctgctt cccaggcctg gtcgaagttc tcatgctcgt cggcccaggc gcagctgatg
     1141 atggtcaact cgttgtggag atggtcgaag gcgagcagga cgtcggtgat catcagggcg
     1201 agatcgggaa ggcccagcgg gtccgggttc ggatcaccga ggggttcgat cgcgcggacc
     1261 aggtcatagc cgaagaggcc gaccgcgcca ccggcaaatg gcggcattcc cggggtctcg
     1321 gcgacccggt accggctcag gtattccttg accccgtcat aggggtccgc ggccggaaac
     1381 gtcgcgatcg gcgcgccctc gccggacatc gcctcgcccg ggaactcctt caactcgccg
     1441 gccacccagc ggatcacccg atggggaccg accccgacga acgagtagcg cccgacctga
     1501 ccgcgctcgg ccgattcgag caggaagcag gggccaccct gcctcagctt gagcagggct
     1561 gaaaccggtg tctcgacatc gtccgtgtac cgcagggcga ccggcaccac gtttgattcc
     1621 ggaaccagcg cccgggcggt ctcaagatcc ggggtg
//
LOCUS       DAOQFI010000539         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_49569_length_1655_cov_2.981875, whole genome shotgun sequence.
ACCESSION   DAOQFI010000539 DAOQFI010000000
VERSION     DAOQFI010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49569_length_1655_cov_2.981875"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLE93_09010"
     CDS             <1..574
                     /locus_tag="PLE93_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HNH87230.1"
                     /translation="ITRKACAGMKKLAIAMDMDETAMSSFRYGSPQPNYDTISMYRNE
                     ILGSQTALKPMLNLFKLARKRGMATFVITARYEPISADPLVGALMGSADLCSSSLAWT
                     GACGLDINTFNFTKVTWANLKDAGYTGLTDLYMRPPDSESKGEVKNSERAEISLRRGY
                     RIIAMFGDQNSDLEKGFYERGFKYQSPEGD"
     gene            594..>1655
                     /locus_tag="PLE93_09015"
     CDS             594..>1655
                     /locus_tag="PLE93_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH87231.1"
                     /translation="MADESFDVIVIGAGPGGYVAAIRAAQLGKTTAVVESDKAGGRCL
                     NYACIPAKTMLHTAEVLDEARNSADIGVKVQGAELDWDALGQRRTDVSASLSGGVKGL
                     FDKNKIRFIEGEGSLTADGDVEVGGTVYGAGKVILATGSVAQAIPGVDFSDRVLDTWG
                     AWSLAEQPKKIAVVGAGASGSEIASAYIRYGTEVILVEMLDQILPVEDKDVVRVVERT
                     FKKQGIEISTGTPVENVEAGKDSVRFTYGENSAEVDYLVIAGGRRPDVDALGLDAAGV
                     KLDENGRVAVDGFQKTSNDKVYAIGDLVRGSALAHKAMEEGVVAAESAAGAETHPVDL
                     DLVPGATFCHPQVASVGLTE"
BASE COUNT          315 a          527 c          551 g          262 t
ORIGIN      
        1 aatcacccgc aaggcctgcg ccgggatgaa gaaactggcg atcgcgatgg acatggacga
       61 gaccgcgatg tccagcttcc gttacggctc gccgcagccg aactacgaca cgatctcgat
      121 gtaccgcaac gagatcctcg gctcccagac cgctctgaag ccgatgctca acctgttcaa
      181 actggcccgg aagcgcggca tggcgacctt cgtgatcacc gcccgctacg agccgatttc
      241 ggccgatccg ctggtcggtg cgctgatggg ttcggccgac ctctgcagca gctcactggc
      301 ctggaccggc gcctgcggcc tggacatcaa caccttcaac ttcaccaagg tgacctgggc
      361 gaacctgaag gacgcgggct acaccggcct gaccgacctc tacatgcgcc cgccggattc
      421 ggaaagcaag ggcgaggtca agaactcgga acgggccgaa atctcgctgc gtcgcggtta
      481 ccggatcatc gccatgttcg gcgaccagaa cagtgacctg gagaagggat tctacgagcg
      541 cggcttcaag taccagagcc ccgaaggcga ctagaagata gtctcgcctg accatggctg
      601 atgaatcgtt tgacgtaatc gttatcggtg cgggaccggg cggctatgtg gccgcgatcc
      661 gcgccgccca gctcggcaag accaccgcgg tggtcgaatc ggacaaggcc ggcggacgct
      721 gtctgaacta cgcgtgcatc ccggccaaga cgatgctcca caccgccgag gttctcgatg
      781 aagccaggaa ctcggcggac atcggggtca aggtgcaggg agccgaactc gactgggacg
      841 cgctcggcca gcgccggacc gatgtctcgg ccagcctgtc cggcggcgtc aagggactct
      901 tcgacaagaa caagatcagg ttcatcgaag gcgaaggcag cctgaccgcc gacggtgacg
      961 tcgaggtcgg tggcaccgtg tacggggccg gcaaggtgat cctcgccacc ggttcggtag
     1021 cgcaggcgat tcccggggtc gatttctccg accgggtgct ggacacctgg ggtgcctggt
     1081 ctctggccga acagccgaag aagatcgccg tggtcggcgc cggtgcctcc ggctccgaga
     1141 tcgcttccgc ctacatccgc tacgggaccg aagtgatcct ggtcgagatg ctcgatcaga
     1201 tcctgccggt cgaggacaag gatgtggtcc gcgtggtcga acgcaccttc aagaagcagg
     1261 gcatcgagat ctccaccggt acgccggtcg agaacgttga ggccggcaag gactcggtca
     1321 ggttcaccta cggcgagaac tcggccgagg tcgattacct ggtgatcgcc gggggccgcc
     1381 ggccggacgt ggacgcgctt ggtcttgacg cggccggggt gaagctggac gagaacggac
     1441 gggtcgccgt cgacgggttc cagaagactt ccaacgacaa ggtctacgcg atcggtgacc
     1501 tggtccgcgg gtcggccctc gcccacaagg cgatggagga gggcgtggtc gcggccgagt
     1561 ccgccgccgg cgccgaaacc catccggtcg atctcgacct ggtcccgggc gccaccttct
     1621 gccacccgca ggtcgccagc gtcgggctga ccgag
//
LOCUS       DAOQFI010000540         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_49724_length_1653_cov_2.499374, whole genome shotgun sequence.
ACCESSION   DAOQFI010000540 DAOQFI010000000
VERSION     DAOQFI010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49724_length_1653_cov_2.499374"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLE93_09020"
     CDS             complement(<1..256)
                     /locus_tag="PLE93_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNH87232.1"
                     /translation="MVIAIDGPAGAGKSTVARGLAAELGFTYLDSGAMYRCVALTCLE
                     WGADPTASAEATSTAESIEIGFDDGDVLIDGVPVTGEIRTP"
     gene            357..1430
                     /locus_tag="PLE93_09025"
     CDS             357..1430
                     /locus_tag="PLE93_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNH87233.1"
                     /translation="MRVQTPGRGPLFPDQGPLGNRVKRRLIGGTAEIAGFILLTVLSP
                     LLMLVGLIVDLALWLKNRRPFVNIRLIPAVWWFLFGEMKAYLKLIYIYVTTGGPWGKG
                     SISRRRQVYQLRIAWARNHLLAIKFLFGLKFELEGMENTGPGPYVLMPRHVSILDNLI
                     GDVFIGHPQRIGVRYVIKEEIRALTPIDIGGRWIPTVFVKRGSLDPDTEIAAVRALTH
                     EMSPGEIMAIYPEGTRPTHKKIRRAQEVIAEKQPGIAPLAARLNHLLPPRLGGPLAIL
                     DEAANGIDIVFCGHVGFEGLRTVGDVMAGVLVGRQIKIRFWRFEGSTIPQGDQARTEW
                     LYEKWQMIDDWVGEELAARGETD"
     gene            complement(1514..1636)
                     /locus_tag="PLE93_09030"
     CDS             complement(1514..1636)
                     /locus_tag="PLE93_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87234.1"
                     /translation="MAMLGAVAGIASREGVTVDGMDAAAVSYPTFQQDLARLQG"
BASE COUNT          285 a          564 c          540 g          264 t
ORIGIN      
        1 ccggagttcg gatctccccg gtcaccggca cgccgtcgat cagcacgtcg ccatcatcga
       61 agccgatttc gatcgactcc gcggtggaag ttgcctccgc cgaggcggtc gggtcggcac
      121 cccattcgag acaggtcagg gccacgcagc ggtacatcgc cccggaatcg aggtaggtga
      181 aaccaagctc cgcggcaagg ccgcgggcaa cggttgactt tccggccccg gccggaccgt
      241 cgatcgcgat caccatgtga atctgccttt catcgaccgc ccatcctacg ggccgggaga
      301 cctgcgggta acccggcggg gcgcggaaag agcaccggcc cgaatacgat cagccgatgc
      361 gcgtccagac ccccggccgg ggcccactct ttcccgacca gggaccgctc ggcaaccgcg
      421 tcaaacggcg cctgatcggc ggcaccgccg aaatcgcggg attcatcctg ctgaccgtgc
      481 tctcgccgct tctgatgctg gtcggcctca tcgtcgacct cgctctctgg ctgaagaacc
      541 gccgcccctt cgtgaacatc cgcctgatcc ccgccgtctg gtggttcctg ttcggggaga
      601 tgaaggccta cctgaagctg atctacatct acgtcacgac cggcggcccc tggggcaagg
      661 ggtcgatttc ccggcgccgg caggtctacc agctaaggat cgcctgggcc cgcaaccacc
      721 tgctcgcgat caagttcctg ttcggcctga agttcgagct cgaaggcatg gagaacaccg
      781 gaccggggcc atatgtcctg atgccccggc acgtcagcat cctcgacaac ctgatcgggg
      841 acgtcttcat cggtcacccg caaagaatcg gggtccgcta cgtgatcaag gaggagatcc
      901 gggcgctgac cccgatcgac atcggcggcc gctggatccc gaccgtgttc gtcaagcgcg
      961 gctcgcttga ccccgacacc gagatcgccg cagtgagggc gctgacccat gagatgagcc
     1021 cgggcgagat catggcgatc tatcccgagg ggacccgccc tacccacaag aagatcaggc
     1081 gggcccagga agtgatcgcc gagaagcaac ccgggatcgc cccgctggcg gcccggctca
     1141 accacctcct gcctccgcgc ctgggtgggc cgctggcgat cctcgacgag gccgccaacg
     1201 gcatcgacat cgtcttctgc ggccacgtcg gcttcgaagg cctgcgcacg gtcggcgacg
     1261 tgatggccgg ggtgctggtt ggccgccaga tcaagatccg cttttggcgc ttcgagggga
     1321 gcaccatccc ccagggcgat caggctcgca ccgagtggct ctacgagaag tggcagatga
     1381 tcgacgactg ggtcggcgag gaactggccg cccgcggcga aaccgactga ggcaaattca
     1441 ggactgtcga gcgcggtgag gtgcgtttgg tcggcggtat tcgccgacca gacgcatctc
     1501 gcgaccgggg gcgctagccc tgaaggcggg cgagatcctg ctggaaggtc gggtagctga
     1561 ccgctgccgc atccatgccg tcgaccgtca cgccttcccg gctggcgatg ccggcgaccg
     1621 cgccgagcat ggcgatgcgg tggtcgccgt ggg
//
LOCUS       DAOQFI010000541         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_49839_length_1651_cov_2.338972, whole genome shotgun sequence.
ACCESSION   DAOQFI010000541 DAOQFI010000000
VERSION     DAOQFI010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49839_length_1651_cov_2.338972"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /gene="meaB"
                     /locus_tag="PLE93_09035"
     CDS             <1..936
                     /gene="meaB"
                     /locus_tag="PLE93_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNH87235.1"
                     /translation="AKPNTKFKGDRALARAITLIESDDPAGWDLVREVYPKTGKARII
                     GFTGPPGVGKSTLIGALTKELRKEDRQVAVLSIDPSSPFTQGALLGDRIRLTEHFLDK
                     GVFIRSMASRGALGGLSEAALQAALVMDASGKDDVLVETVGVGQGEIDIVDHADTIVL
                     ALMPGSGDSIQALKAGVMEIPDIIVVNKSDHPMTDTMIREVRGALSLAHTPGGWRVPI
                     LRTEAARGEGIEELAAKVAEHRAFIENEGTLAERRARNLRSEVLGLATARLRRRLEAA
                     IEDDPRVVELLNRVVSRELDPASAAAELLEGEIDE"
     gene            929..1330
                     /locus_tag="PLE93_09040"
     CDS             929..1330
                     /locus_tag="PLE93_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87236.1"
                     /translation="MSEKSSPQPSLEDVKAWEGYRLDDMTGQGVARVEGLFVDGESGQ
                     PTWVLVKLGRFGKVVPISIRECAAAAGRVWTPYDREVIRNAPAVDPALPLNREQEQQV
                     LEYYGIPETVGRGAELKDRPAGSTTANPITT"
BASE COUNT          307 a          520 c          561 g          263 t
ORIGIN      
        1 gcgaagccca acacgaagtt caagggcgac cgcgccctcg cccgggcgat cacgctgatc
       61 gaatccgatg accctgccgg gtgggatctg gtccgcgagg tctacccgaa gaccggaaag
      121 gccaggatca tcgggttcac cggtccgccc ggggtcggca agagcacttt gatcggcgct
      181 ctcaccaagg agctccgcaa ggaggaccgc caggtggcgg tcctttcgat cgacccgtcg
      241 agcccgttca cccagggtgc cctgctcggt gaccgaatcc gcctgaccga gcacttcctt
      301 gacaagggtg tgttcatccg gtcgatggca tcccgcgggg cgctcggcgg gctttccgag
      361 gccgcgcttc aggctgccct ggtcatggac gcttccggca aggacgacgt cctggtcgag
      421 acggtcgggg tggggcaggg ggaaatcgac atcgtcgacc atgccgacac gatcgtcctc
      481 gccctgatgc ccggctccgg tgattcgatt caggcgctga aggcgggtgt catggagatt
      541 ccggacatca tcgtggtcaa caaatccgac cacccgatga ccgacacgat gattcgcgag
      601 gtgcgcggcg cgctctcgct cgcccacaca ccgggtggct ggcgggtgcc gattcttcgc
      661 accgaggcag ctcgcggcga gggaatcgaa gaacttgccg caaaagtggc ggaacaccgc
      721 gccttcatcg agaatgaagg gacgctggcc gaaaggcggg cccgcaatct gcgctcggag
      781 gtgctggggc tggccacggc caggttgcgt cggcggctcg aagcggcaat cgaagatgat
      841 ccccgtgtgg tggagttgtt gaaccgagtg gtcagccggg aactggaccc ggcgtcggcc
      901 gcggccgaac tgctggaagg agaaatcgat gagtgagaag tcttccccgc aaccgagtct
      961 cgaggatgtc aaggcctggg agggttaccg gctggatgac atgaccggcc agggtgtagc
     1021 tcgggtcgaa ggcctgttcg tcgacggcga gtccggccag ccgacctggg tgctggtcaa
     1081 actgggccgc ttcggcaagg tggtcccgat ctcgattcgc gaatgcgcgg ctgccgccgg
     1141 ccgggtctgg actccctacg accgcgaagt cattcgcaac gcgcccgcgg tcgaccccgc
     1201 cctcccgctc aaccgggaac aggagcagca ggtactcgag tactacggaa tcccggaaac
     1261 ggtgggccgc ggagccgaac tcaaggaccg ccccgccggc tcaaccaccg caaacccgat
     1321 caccacctga cccgaacgcc cagaggagcc aggcgaaacg ccaagcagga gccaggcagg
     1381 tccggaaagc aatcgaggcc aggcaggccg ggaaaccaac cgaggccaca ggcaggtccg
     1441 gaaatcccgt ggcgagacgc aacgttgcgt ctcgttgaga tttgacgagt tgtcaaatcc
     1501 tcgggctgcc gaaccccgat caccacgttt tgtcaagtcc ccgcgagacg caacgttgcg
     1561 tctcgccagt gtttttttcg atgtgttgcg ggcccgaccg gttgggtccg gtccgaccgg
     1621 gtcccgtcgg gtcgggttgg tctgacgggg g
//
LOCUS       DAOQFI010000542         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_50097_length_1646_cov_3.246386, whole genome shotgun sequence.
ACCESSION   DAOQFI010000542 DAOQFI010000000
VERSION     DAOQFI010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50097_length_1646_cov_3.246386"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /gene="coaBC"
                     /locus_tag="PLE93_09045"
     CDS             <1..860
                     /gene="coaBC"
                     /locus_tag="PLE93_09045"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNH87237.1"
                     /translation="LVAPAMNERMYLSGATQGNLSRLRQMGVEVIDPGVGRLASKGEA
                     GVGRLPEPTELLERVEAALDEEKSADLEGLRVLVTAGGTREGIDPVRYIGNRSSGRMG
                     LALAERARKRGAEVTVICANVNLPAPAGVRRVDVESTVDLARACREQFPAHDLLLMAA
                     APADFTVADTSNEKLSRTGGEFTLTLVPTEDILAGLAANRIDGQTVVGFAAQHGGDAI
                     GRARQKLERKGADMIVLNDVSDRTIGFDSDENAVTLVTGSGEREIPKAPKTEIADRIL
                     DRAVELRAG"
     gene            860..1276
                     /gene="dtd"
                     /locus_tag="PLE93_09050"
     CDS             860..1276
                     /gene="dtd"
                     /locus_tag="PLE93_09050"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNH87238.1"
                     /translation="MRALVQRVTRASVAVEGEIVSEIGPGMLVLLGVKKGDTEAEAEK
                     VAAKLLKLRVFEDGEGRMNEPPGEREILCISQFTLYGDTRKGNRPSWIEAAGGEEAEP
                     LYDLVCEKLDAKKGIFGADMKVELVNDGPVTLLVET"
     gene            1458..>1646
                     /locus_tag="PLE93_09055"
     CDS             1458..>1646
                     /locus_tag="PLE93_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNH87239.1"
                     /translation="MNPTPEDIEKRLEAIDPEIEFLALESAGGDALRLFIDRPGGVTL
                     DICQQVTSGLQDVLVDHSL"
BASE COUNT          302 a          515 c          562 g          267 t
ORIGIN      
        1 ggctggtcgc cccggcgatg aacgagcgga tgtatctctc gggagccacc caggggaacc
       61 tttcccggct gcggcagatg ggggtggagg taatcgatcc cggggtcggc cgcctcgctt
      121 cgaagggcga agccggggtc ggccgcctgc ccgagccaac cgagctgctg gaacgggtcg
      181 aggccgccct cgacgaggag aagtcggcgg acctcgaggg tctgcgggtg ctggtcacgg
      241 ccgggggcac ccgcgagggg atcgatccgg tccgctacat cggcaaccgc tcctccggcc
      301 ggatggggct tgccctggcc gagcgggccc gcaagcgcgg ggcggaggtg accgtgatct
      361 gcgccaacgt gaacctgccg gccccggcgg gagtgagaag ggtggatgtc gaatcgacgg
      421 tcgacctcgc ccgggcatgt cgcgaacagt tcccggccca tgacctgctg ctgatggcgg
      481 cggcgccggc ggacttcacc gtggccgata cctcgaacga aaagctgtcc cggaccgggg
      541 gcgagttcac cctgaccctg gttccgaccg aggacattct cgccggcctc gccgcgaacc
      601 ggatcgatgg tcagaccgtg gtcggtttcg ccgcgcagca tggtggcgac gcgattggcc
      661 gggcccggca gaagctggag cgaaaagggg cggacatgat cgtcctcaat gacgtatccg
      721 accggacgat cggcttcgac tcggacgaga acgcggtgac gctggtcacc gggtccggcg
      781 agcgggagat ccccaaggca ccgaagaccg agatcgccga ccgcatcctc gaccgtgcgg
      841 tcgaactccg cgccggctga tgcgggcact ggttcagcgg gttacccggg cctcggtcgc
      901 ggtcgaaggc gagatcgtct cggagatcgg cccggggatg ctcgttctgc tgggagtgaa
      961 aaagggcgac accgaagccg aggccgaaaa ggtcgcggcc aaactgctca aactccgggt
     1021 cttcgaagac ggcgaaggcc gcatgaacga accgcccggc gaacgcgaga ttctctgcat
     1081 tagccagttc accctctacg gcgacacccg caagggcaac cgcccgagct ggattgaagc
     1141 cgcgggcggg gaggaagccg aaccgcttta cgacctcgtc tgcgaaaagc tcgacgcgaa
     1201 gaagggcatc ttcggtgccg acatgaaggt cgaactcgtg aacgacggcc cggtcaccct
     1261 tctggtcgaa acctgacccg gagaacaccg cgagatccga gtttttcgtc actgtgacga
     1321 aaaagtcgga gcccgctgtt cctggttgct gcccgacttg atattcgggc tatacttgtt
     1381 ggtgcttttc ccctctagct gtgtcgaaaa gtgggcctcg cccgcttttt tttggtcctg
     1441 aacccggaaa tacagacttg aatcccaccc ccgaagacat cgagaaaagg ctcgaagcga
     1501 tcgatcccga gatcgagttt ctggcgctgg aaagcgccgg aggtgacgcc ctgcgccttt
     1561 tcatcgatcg gccgggcggg gtgaccctgg acatctgcca gcaggtgacc tccggacttc
     1621 aggacgtcct ggtcgaccac tcgctc
//
LOCUS       DAOQFI010000543         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_50169_length_1645_cov_2.484906, whole genome shotgun sequence.
ACCESSION   DAOQFI010000543 DAOQFI010000000
VERSION     DAOQFI010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50169_length_1645_cov_2.484906"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLE93_09060"
     CDS             complement(<1..739)
                     /locus_tag="PLE93_09060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH87240.1"
                     /translation="MVGGGLTGLWAAVRAKERNPAREVVLIEREQLATGASGRNGGFM
                     SSSLVHGLGNGLARFPDEVPLLEKLGLENLAGIEKVIEEQGIDCDLRKPGAIEVAVTD
                     AQVADLQGGVEELAGYGHEAVMLDGEEMRAEVNSPIYRGGLWQKSGEWSVDPVRLCDG
                     LAAYAERLGVRIHESTPMEGFEETGGGVEVRTANGSIKTERVLLATSAFRVPVRQIRH
                     RVIPVYDYVLVTEPLTRAQWVSLGWQNH"
     gene            1012..>1645
                     /locus_tag="PLE93_09065"
     CDS             1012..>1645
                     /locus_tag="PLE93_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH87241.1"
                     /translation="MNFKPISEHELARLKDDELISHAVAARDARKQDAFEETLKLFVG
                     RRFGLVEYWVSRKSNANDRDEIVSRAFVSIIKGIGKFRGRSTGQMVEWMRTVTGRRIA
                     DFYRDKEKTPRMVPLGEQPKEDEDWGPTNLGNPDDTGGVEVEMLIEQTLETRNEVQQE
                     VIRLRMKGYSSKEVAEMSSDQKMSFTNVDKIFSRFRQDIEKAMSVDEVSKA"
BASE COUNT          325 a          526 c          514 g          280 t
ORIGIN      
        1 gatggttctg ccagccgagc gagacccact gggcgcgggt cagtggctcg gttaccagca
       61 catagtcgta gaccgggatc acccggtggc gaatctgccg gaccggcacc ctgaaggccg
      121 aggtcgcgag cagcaccctt tcggtcttga tcgagccgtt cgcggtgcgg acttcgactc
      181 cgcccccggt ttcctcgaag ccttccatcg gggtcgactc gtgaattcgc accccgagcc
      241 gttcggcata ggcggccagg ccgtcgcaaa gccggaccgg atcgaccgac cactcgcctg
      301 acttctgcca gaggccgccg cggtagatcg gcgagttcac ttcggccctc atctcctcgc
      361 cgtcgagcat cactgcctcg tgcccgtacc cggccagctc ttcgaccccg ccttgaaggt
      421 cggccacctg ggcgtcggtc accgcgacct cgatcgcccc gggcttcctc aggtcacagt
      481 cgatcccctg ctcctcgatt accttctcga tcccggccag attctccagg cccagcttct
      541 cgagcagcgg cacctcatcc gggaagcggg cgaggccatt gccgaggcca tggaccagcg
      601 aggaagacat gaagccgccg ttgcgtccgc tcgccccggt cgcaagctgt tcacgctcga
      661 tcagcacgac ctcccgggcc gggttgcgtt ccttcgcccg gaccgccgcc cagagcccgg
      721 tcagcccgcc cccgaccacg accagatccg cctcggagga accttcgaga gggtggtgcg
      781 gttcgggtcg cggcccggaa agccagaacg gcgaccgctc ggcttcggca aaggagcgac
      841 gatcggattc acctggttcc gattcgggcc agactgcgtt ctgtgcgccg cgggagatgg
      901 gctgactgta gccgacccag ccggtatctt ggcacttggt tccgggcgtc cgcgagcgga
      961 tttcagggcc atgtcagaag gaatgcgaag gcaacgacct ccgggacaag cttgaacttc
     1021 aagccgatca gtgaacatga actggcgaga ctgaaagatg acgaactcat ctcccacgct
     1081 gttgctgccc gggatgcgcg caaacaggac gctttcgagg agaccctcaa actctttgtc
     1141 gggcgccgct tcgggcttgt cgagtactgg gtcagcagga agtccaatgc caacgaccgg
     1201 gacgagatcg tcagccgggc gttcgtcagc atcatcaagg gcattggcaa gtttcggggc
     1261 cggagtaccg gccagatggt cgagtggatg cgaaccgtca ccggacggcg gatcgccgac
     1321 ttctacaggg acaaggaaaa gacccccagg atggtcccgc tgggcgaaca gccgaaggaa
     1381 gatgaggact gggggccgac gaacctcggc aaccccgatg acaccggcgg cgtcgaggtc
     1441 gaaatgctga ttgagcagac tcttgaaacc cggaacgagg tccagcagga agtgatccga
     1501 ttgcggatga aagggtattc ctcgaaagag gttgccgaaa tgagcagcga ccagaaaatg
     1561 tctttcacca acgtcgacaa gatcttctcc cgttttcggc aggacatcga aaaggcaatg
     1621 agtgtcgacg aggttagcaa ggcta
//
LOCUS       DAOQFI010000544         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_50240_length_1644_cov_2.438641, whole genome shotgun sequence.
ACCESSION   DAOQFI010000544 DAOQFI010000000
VERSION     DAOQFI010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50240_length_1644_cov_2.438641"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLE93_09070"
     CDS             complement(<1..672)
                     /locus_tag="PLE93_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87242.1"
                     /translation="MSEHLLIGSIGDSRGAERLAAERVLESAAGRVGVVLETGRERPR
                     PASLLAPGEAKELESELLEALPELEPKARGSLVYLSAAQSERLAGEVISLREIVDERP
                     LAVIAGAGRYRELLELGWDRVVIRSDEAGQDAPEVDAPVSGDAVAGPGALTGLVVEEA
                     GEFADRVELVRGAAGKLWPAGGRLLGRLRSTGGQATPLVLGCVFALVLATVAVVAISG
                     AVTGKA"
     gene            complement(669..1001)
                     /locus_tag="PLE93_09075"
     CDS             complement(669..1001)
                     /locus_tag="PLE93_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87243.1"
                     /translation="MNSGRSGQATVEAVCGIAALMLAGLLCFQLLATGYTATIADGAA
                     EAGAVALIRGEPVEPAVLEALPGWARDRVSVSRVGSRVKVRLRPPSLFSSMSEKLAVS
                     AEAGGRPG"
     gene            complement(1001..1360)
                     /locus_tag="PLE93_09080"
     CDS             complement(1001..1360)
                     /locus_tag="PLE93_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87244.1"
                     /translation="MDADREKAGSGGIGSRRGSVSLELIGSLPIILLAVLVAAQIAIA
                     GAALWSAGVAARAGARAVLTGNSPRGAAEHALPSVLQGGLKVSQEDGVKVGVRVPGLL
                     PGFPETHVYGSSSLGDG"
     gene            complement(1430..>1644)
                     /locus_tag="PLE93_09085"
     CDS             complement(1430..>1644)
                     /locus_tag="PLE93_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87245.1"
                     /translation="SSDVFDRLEGEFGLAGTTAPGSIDHLLSRGVEVLEQPEAWPPAR
                     RDVPDPETGLMVRLSDHAPVLARIRV"
BASE COUNT          270 a          653 c          518 g          203 t
ORIGIN      
        1 tgccttgccg gtcaccgccc cggaaatcgc gaccaccgcc acggtcgcca gcaccagtgc
       61 gaagacgcaa ccgagcacca gcggggtcgc ctgcccgccg gtcgaacgga gccggcccag
      121 gagccgcccg ccggcgggcc acagtttccc ggccgcgccc cgcacgagtt cgacccgatc
      181 ggcgaactcg cctgcctctt cgacgaccag cccggtcagg gcgccggggc cggcgacggc
      241 atctccggaa accggcgcat cgacctcggg ggcatcctgt ccggcctcgt cggatctgat
      301 caccacccgg tcccagccga gttccagcag ctcgcggtac cggccggccc ccgcaatcac
      361 ggccaggggc cgttcatcca cgatctcccg caagctgatc acttccccgg ccagccgctc
      421 tgactgggcg gctgacaggt agacgaggct cccgcgggct ttcggctcaa gctccggcag
      481 ggcctccagc agttccgatt ccagctcctt ggcctcgccg ggagccagca ggctggccgg
      541 ccggggccgc tcccgaccgg tctccagcac cacgccgacc cgcccggccg ccgattccag
      601 aacccgttcg gcggcgagcc gttcggcgcc acgcgaatcg ccgatcgacc cgatcagcag
      661 atgctcgctc atcccggcct cccgcccgcc tcggccgaaa ccgcaagctt ttcggacatc
      721 gaggagaaga gcgaaggcgg ccggagccgg acctttaccc gggacccgac cctcgacacc
      781 gagacccggt cccgggccca gccgggcaat gcctctagca cggccggctc gaccggctcg
      841 ccccggatca gcgcgaccgc gccagcctcg gccgccccgt cggcgatggt cgcggtgtaa
      901 ccggtggcaa gcagctggaa gcagagcagg ccggccagca tcagggcggc gatgccgcag
      961 accgcctcga ccgtcgcctg gcccgagcgc ccggaattca tcagccatct cccagcgaac
     1021 tcgaaccgta gacatgggtc tccgggaacc cgggcagcag cccgggcacc ctcaccccga
     1081 ctttgacccc gtcttcctgc gagaccttca ggcccccctg caggaccgat ggaagcgcgt
     1141 gttcagccgc cccgcgcggg gagttgccgg tgagcaccgc ccgggccccg gcccgggcgg
     1201 ccacccccgc cgaccagagt gccgcccccg cgatcgcgat ctgggccgcg accagcacgg
     1261 ccagcaggat gatcggcagg gaaccgatca actcgagcga gaccgagccc cgccgcgaac
     1321 cgatcccgcc cgagcctgcc ttttcgcgat ccgcatccat gcccggaccg tggcaggaaa
     1381 aagctcacat gtggctctac aactgcaacg atttgcaacg tcggggtgct cagacccgaa
     1441 tccgggcgag caccggtgcg tgatcggaga gcctgaccat caggccggtc tccggatcgg
     1501 gcacgtcgcg ccgcgccggc ggccaggctt ccggctgctc aagcacttcc accccgcggg
     1561 aaagcaggtg gtcgatcgaa cccggcgcag tggttccggc cagcccgaac tcgccttcga
     1621 gccggtcgaa aacatcgctc gaac
//
LOCUS       DAOQFI010000545         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_50299_length_1643_cov_2.732368, whole genome shotgun sequence.
ACCESSION   DAOQFI010000545 DAOQFI010000000
VERSION     DAOQFI010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50299_length_1643_cov_2.732368"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..867)
                     /locus_tag="PLE93_09090"
     CDS             complement(85..867)
                     /locus_tag="PLE93_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNH87246.1"
                     /translation="MALADSYDGFLIDLDGVVWLGHEPIEGAAKAIAALAAAGKPFVF
                     VTNSPRVTPAEQARILREQGIEADEDRVVTAGDTLLELTRKEIGEAGKVYAIGTSGFL
                     EQVEGAGFQLLPAERWQEAEAVLVTGHPRFDYDELKAASMAARAGAFLATIGRDPTMP
                     MPDGLWPGTGSIVAAVEAASGRTAVVAGKPERPIFDLGVRRLGLPEGARVAMIGDRLD
                     TDVGGAQKAGLEGILVAGNESHNPETQVTPNHNISSLLDLTD"
     gene            946..1437
                     /locus_tag="PLE93_09095"
     CDS             946..1437
                     /locus_tag="PLE93_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH87247.1"
                     /translation="MTGNRTHTEGPPPFETALEDHGPRLHAWLLARVGPSRADDVFQE
                     TMLAALAAWDRVPVQSVRAWLFTIARNKAIDEQRRDANRPRPEGEVDSWPSAEVAERL
                     EDPVWDQVRELPEKQRIAVTLRFRGDLNHREIGTVMEISEAAARRNVFEGVKRLRERI
                     EDE"
     gene            1430..>1643
                     /locus_tag="PLE93_09100"
     CDS             1430..>1643
                     /locus_tag="PLE93_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87248.1"
                     /translation="MSEIEARLAAGEVAGTGSNWEEIRTSLLRQADREGAIDLAYERH
                     DTPFGTMVIVAGEGGILRIALAAEPET"
BASE COUNT          267 a          543 c          573 g          260 t
ORIGIN      
        1 cagccgcgct ggtcctggag tgtcccggac gacgctcacc cgaccccgcg gctgcgccta
       61 cccggcggat tttcgcggga gggctcagtc ggtcaggtcg aggaggctgg agatgttgtg
      121 gttgggggtg acttgggttt cggggttgtg gctttcgttg ccggcgacga ggattccttc
      181 gaggccggcc ttctgggcgc cgccgacatc ggtgtcgagg cggtccccga tcatcgccac
      241 ccgggcccct tccggcaggc cgagccgccg gacgccgaga tcgaagatcg ggcgttccgg
      301 cttgcccgcc accaccgcgg tccggcccga ggccgcttcg accgcggcga cgattgaacc
      361 cgtcccgggc cagaggccat ccggcatcgg catggtcggg tcgcggccga tcgtcgcgag
      421 gaaggcgccg gcccgcgccg ccatcgaagc ggccttcagt tcgtcgtaat cgaagcgggg
      481 atggccggtc accagcaccg cctccgcctc ctgccagcgt tcggcaggaa gcagctggaa
      541 acctgcgcct tcgacctgct ccagaaatcc gcttgtaccg atcgcataga ccttgccggc
      601 ctcgccaatc tccttccggg tcagctccag cagggtgtcg cccgcagtca ccacccgatc
      661 ttcgtcggct tcgattccct gctcgcgcag aatcctcgcc tgctcggccg gcgtcacccg
      721 ggggctgttc gtgacgaaga cgaacggctt gcccgcggca gccagcgcgg caatggcctt
      781 cgccgctcct tcgatcggct cgtgaccgag ccagacgacc ccgtcgaggt cgatcaggaa
      841 gccgtcgtat gagtcggcca gggccacgag ccaacaatag agttgcggca tgagtccggt
      901 cggcggcgat ctcacaaaac ctgcctcggc ttcgtctcag gggcagtgac cggaaatcgc
      961 acccacaccg aggggccgcc cccattcgag acggcccttg aggatcacgg gccgaggctg
     1021 catgcctggc tgctcgcgcg agtcggaccc tcccgggccg acgatgtctt tcaggagacg
     1081 atgctcgcgg cgctggccgc ctgggacagg gtcccggttc agtcggtgcg cgcgtggctc
     1141 ttcacgatcg cccggaacaa ggcgattgac gagcagcgcc gggatgcgaa caggccccgg
     1201 ccggaaggcg aggttgacag ttggccttcg gccgaggttg ccgagcgcct ggaggatccg
     1261 gtctgggatc aagtgcggga gctgccggag aaacagcgga tcgcggtgac ccttcgcttt
     1321 cgcggtgatc tgaaccaccg tgaaatcggg accgtgatgg agatctccga agcggccgcc
     1381 cgccgcaacg tgttcgaagg cgtcaagcgc ctgcgggaaa ggatcgaaga tgagtgagat
     1441 cgaagcgaga ctggccgccg gcgaagtggc cgggaccgga agcaactggg aggagatccg
     1501 gaccagcctg cttcggcagg cagaccggga gggtgccatt gacctcgcct acgagcggca
     1561 tgacacaccg ttcgggacga tggtgatcgt cgcgggggaa gggggcatcc tccggatcgc
     1621 cctcgccgcg gaaccggaaa ccg
//
LOCUS       DAOQFI010000546         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_50513_length_1639_cov_3.530934, whole genome shotgun sequence.
ACCESSION   DAOQFI010000546 DAOQFI010000000
VERSION     DAOQFI010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50513_length_1639_cov_3.530934"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLE93_09105"
     CDS             <1..606
                     /locus_tag="PLE93_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87249.1"
                     /translation="TVESPEAKPTEPEAAVEPPAPAQEGDAGDMLEAERHHLASHPTE
                     HYDVDAAIAEEDEIDVLSESSLSDELDRALESGETAVAGGETVEYDMSSEPDSGEFEF
                     DEPDSAEMESAEHEAEPESAGQEAVEGDSAELLYQDGATEDQPVDQDEPETGEEDAEG
                     SSEFYDQNDVLEGTPDFLEETPEHDQLWFEQKEPKDFDFGD"
     gene            complement(636..1490)
                     /locus_tag="PLE93_09110"
     CDS             complement(636..1490)
                     /locus_tag="PLE93_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH87250.1"
                     /translation="MAIDDLASTLDHRDNRATGESVVRARGVNVFRWSTALRQRVMLL
                     EEIEWRVDRGQHWVMLGPNGAGKTTLLHLAAADSHPSEGTVEVLGKRLGNTDMRALRE
                     AIGLVDARTSRAIPSRRPVLETIESGAFNSIALQHQRLDASHRDRARELMAVVGLSDL
                     ATRAFGECSQGERQRILLARALMPSPELLLLDEPAAGMDLPSREQLVSALVSMAEGDP
                     GLTTVTVTHHLEEIPPTATHALLIDHGRIVAQGPIDEALTSESVSAGFGLPIDVRKAS
                     GRWTAAIS"
     gene            1554..1626
                     /locus_tag="PLE93_09115"
     tRNA            1554..1626
                     /locus_tag="PLE93_09115"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1587..1589,aa:Ala,seq:tgc)
BASE COUNT          298 a          522 c          571 g          248 t
ORIGIN      
        1 acggttgaat cacccgaggc gaaaccgacc gaacccgaag cggcggtcga gccgccggct
       61 cccgcccagg agggcgatgc cggggacatg ctcgaggccg aacgccacca ccttgccagc
      121 catccgaccg agcattacga cgtcgatgcc gcgatcgccg aggaggacga gatcgacgtg
      181 ctcagcgaga gttcactctc ggatgaactc gaccgggccc tggaaagcgg cgaaaccgcc
      241 gtggccgggg gcgagaccgt cgagtacgac atgtcatcgg aaccggactc gggcgagttc
      301 gagttcgatg aacccgactc ggccgaaatg gaatcagccg aacatgaagc ggagccggaa
      361 tcggccgggc aggaagcggt cgaaggggat tcggccgagc tgctctacca ggatggcgcc
      421 accgaggatc agccggtcga tcaggacgag ccggaaaccg gtgaagaaga cgccgagggt
      481 tcgagcgagt tctacgatca gaacgatgtg ctcgagggca ccccggactt tctcgaagaa
      541 acgcccgagc acgaccagct ctggttcgag cagaaggaac cgaaggactt cgacttcggc
      601 gactgagctg gttctgccgg tcgccgtcga accagtcagc ttatggccgc cgtccagcgg
      661 cctgacgctt tgcgaacatc gatcggcagg ccgaaaccgg cagacactga ttcagaggtg
      721 agtgcctcgt cgatcgggcc ctgggcgacg atccggccgt gatcgatcag cagtgcgtgg
      781 gtggcggtcg gcgggatctc ttccaggtgg tgggtgacgg tgaccgtggt caggccgggg
      841 tctccttcag ccatcgagac cagcgcggaa acgagctgct cgcgcgaggg cagatccatg
      901 ccggcagccg gctcgtcgag gagcagcagc tcagggctcg gcattagcgc ccgggcgagc
      961 aggatccgct gccgctcccc ctgcgagcac tcgccgaagg cccgggtggc gaggtcgctc
     1021 aaaccgacga cggccatcaa ttcccgggcc cggtcgcggt gggaagcgtc caggcgctgg
     1081 tgctggagag cgatgctgtt gaaagctccg gactcgatcg tctcgaggac cgggcggcgg
     1141 gaggggatcg cccgcgaggt gcgggcgtca accagtccga tggcttccct cagcgcccgc
     1201 atgtcggtgt tgcccagccg cttgccgagc acttcgaccg taccttcgga agggtgggaa
     1261 tcggcggcgg ccaggtggag gagggtcgtc ttgccggcgc cgttcgggcc gagcatcacc
     1321 cagtgctggc cgcggtccac gcgccactcg atttcctcca gcagcatcac ccgctgtcga
     1381 agtgcggttg accagcgaaa gacgttcacc cccctggccc ggaccacgga ttcgcccgtt
     1441 gcccggttgt cccggtgatc gagggttgag gcaaggtcgt cgatcgccac cggctcaggc
     1501 tatctgcggc agggtccggc cgcccccgaa cccgtacaat cgccgtccac tctggggcca
     1561 tagctcagtt ggcagagcgc ctgctttgca agcaggaggt cgccggttcg atcccggctg
     1621 gctccactcg cgtgaccgg
//
LOCUS       DAOQFI010000547         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_50625_length_1637_cov_3.811631, whole genome shotgun sequence.
ACCESSION   DAOQFI010000547 DAOQFI010000000
VERSION     DAOQFI010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50625_length_1637_cov_3.811631"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1637
                     /locus_tag="PLE93_09120"
     CDS             <1..>1637
                     /locus_tag="PLE93_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87251.1"
                     /translation="KALKTARQIVIARERKNPHRKRIRELTEPELRAVKRSETCSVAE
                     QRAIRKIPAIQWLVAYLLTAGRTRPFLNRLLVTAALFEMAFTVRPEFRFVFSRFRSGD
                     SINWAHGSPQGPFTIEANYHALRKVTDCGKSGATIHINLDLLDQIAATKVAGELRFPN
                     FGRFCAIDGTLIEANVPQKLPRMRSPRARKTLIREIAGKHRRMADMVFHGFGKHSDAI
                     DDQPEPGLTIRKRCFGYNLVVISCLDTCLPVIWTLMPATGNEVAAARELIEALFELRP
                     DFPMEYLVGDGLYNRGFEFPSTLYKNYGIHLVSPRTNRKSKSEWADSDGVPRCAHGLM
                     KFTRHGEVWDAAKRRAHGIRPGNLPPNGKGLNPRLRFECHEEVCREKSTRMEDDWILH
                     TFLPHIGQDKWTALREVLLARRNTSESLFAQLKQLGVGAKWPNRARWATDNGMRWLTS
                     LALLNMTARKLVHLDGSYAAALKDAYSAGIITRQQMHRFLEVEGIETDSRSRADDTPP
                     VAPSTWNDWIELPIDREFLVTPVDAYSDIPYGSNKSE"
BASE COUNT          363 a          533 c          463 g          278 t
ORIGIN      
        1 caaagccctc aagacagctc gtcagatcgt catcgcgaga gagaggaaga acccgcaccg
       61 caaacgaatc cgggaactca cagagcccga gctcagagcc gtcaagagat ctgagacttg
      121 ctcggtcgct gagcagcgag ccatcagaaa aattccggcc attcagtggc tcgttgccta
      181 tctcctgacc gcgggaagga cccgtccgtt ccttaaccga ttgcttgtca cagcggctct
      241 tttcgagatg gcgttcacgg tccgtccaga gttccggttc gtgttctcca gattccgaag
      301 tggcgactcg atcaactggg cgcatggaag tccccaaggc ccgttcacga tcgaagccaa
      361 ctaccacgcc ctgcggaaag tcactgactg tggaaagtcg ggggcaacca tccacatcaa
      421 cctggatctc ctcgaccaga tcgccgcgac gaaggtagcc ggcgaactcc ggttccccaa
      481 cttcggtcgg ttctgtgcca tcgacggaac actgatcgag gcgaacgttc cccagaaact
      541 cccaaggatg aggtcccctc gcgccaggaa gactctgatc cgcgagatcg cgggtaagca
      601 tcggcgaatg gccgacatgg tcttccacgg cttcggaaag cactccgacg ccatcgacga
      661 tcagcccgaa cccggtctca caattcggaa gcgttgcttc ggctacaacc tcgtcgtcat
      721 cagctgtctc gacacctgcc tgccggtgat ctggacgctt atgccggcaa caggaaacga
      781 ggtcgccgcc gcgagggaac tcatcgaggc tcttttcgag ctgcggcctg acttcccgat
      841 ggagtacctg gtcggggatg gcctctacaa caggggcttc gaatttccga gcaccctcta
      901 caagaactac ggcatccacc tggtctctcc aagaaccaac aggaagtcga agagcgaatg
      961 ggccgacagc gacggtgtgc cccggtgcgc acacggcctc atgaagttca cccgacatgg
     1021 cgaggtctgg gatgccgcga agcggagagc tcatggcatt cgcccgggaa accttcctcc
     1081 aaacgggaag ggcctgaacc cgcggcttcg gttcgagtgc cacgaggaag tctgccgaga
     1141 gaagagcacc cgcatggagg acgattggat cctccacacc ttcctgcccc atatcgggca
     1201 ggacaaatgg accgcactca gggaagtcct gctggcgagg cgtaacacat ccgagtccct
     1261 cttcgctcag ctcaagcaac tcggcgtcgg agcaaagtgg ccgaaccgag ccaggtgggc
     1321 caccgacaac ggcatgcggt ggctcacttc gctggccctc ctgaacatga cggcccgcaa
     1381 gctcgtccac ctcgacggga gctacgccgc cgccctcaag gacgcctaca gcgccggcat
     1441 catcacccgg cagcagatgc accgattcct cgaggttgag ggaatcgaaa cggactcccg
     1501 aagtcgtgcg gatgacaccc cgccagtcgc gccgtccacc tggaacgact ggatcgaact
     1561 cccgatcgat cgggagttcc tcgtgacccc cgtggatgcc tacagcgaca ttccctacgg
     1621 ctccaacaag tcggaga
//
LOCUS       DAOQFI010000548         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_50697_length_1636_cov_2.948134, whole genome shotgun sequence.
ACCESSION   DAOQFI010000548 DAOQFI010000000
VERSION     DAOQFI010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50697_length_1636_cov_2.948134"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLE93_09125"
     CDS             <1..997
                     /locus_tag="PLE93_09125"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNH87252.1"
                     /translation="AIAARENGASKKVARRIVLAGMANYRRVMRELAEKSYLDVWYER
                     LDVERMIEFLEEENGTDWGVSLRRDTRKASRKDSSRAQRKLTEGSAEGEPRFASRPPL
                     LVPASELAASLGVNDIDMIWGYLEQLMGQYADTLAADRKYLFEHYEFVDMARKVVGVG
                     SVGTRAWVLLFISRRRKDPLVLQVKEAQASVLAPYAGASKYRLQGRRVVEGQRLTQAA
                     SDLFLGWIRAKGPDGNERDFYIRQLWDGKLSIDIGSLDELGLTEYVRACGGTLALAHA
                     RTGDRVGIADYLGKNDSFDQAIADFSLSYAKQNQEDFKAFTAEIEAGRIDATPGI"
     gene            1023..>1636
                     /locus_tag="PLE93_09130"
     CDS             1023..>1636
                     /locus_tag="PLE93_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87253.1"
                     /translation="MKSRPFFRLLILVLLALALVPASAGASVAGAAKRLDNLRDQPQR
                     LRQFLLGMPKGTDLHTHLSGAVYAEAMIRWGAEDGKCVDPVTFVSTFPPCTGSQVPLT
                     TALTNTQLWDQILAAWSMREFRPDAFSGHNHFFATFDLFGSAFSGRQGDGLAQVTQRA
                     ASQNVQYVEPLITPQFGATLAIANQVGYIPDFAEMREAMLNAGIT"
BASE COUNT          282 a          565 c          530 g          259 t
ORIGIN      
        1 cgcgattgcc gcccgggaga acggcgccag caagaaggtc gcccgcagga tcgtcctggc
       61 cgggatggcc aattaccggc gggtgatgcg tgaacttgcc gaaaagagct atctcgatgt
      121 ctggtacgag cggcttgacg tcgagcggat gatcgagttc ctcgaagagg agaacggaac
      181 cgactgggga gtcagcctcc gccgggacac ccggaaggcc agccggaagg acagcagccg
      241 ggcccagcgc aagctgaccg aaggctcggc ggagggcgag ccgcggttcg ccagccggcc
      301 gcccctgctc gtccccgcat cggagctcgc cgcgtcactg ggcgtaaacg acatcgacat
      361 gatctgggga tatctcgagc agctgatggg ccagtacgcc gacaccctcg ccgccgaccg
      421 caaatatctc ttcgagcact acgaattcgt cgacatggcc cgcaaggtgg tcggggtcgg
      481 ctcggtcggc acccgggcct gggtgctgct cttcatttcc cgccggcgca aggacccgct
      541 ggtgcttcag gtcaaggaag cccaggcgtc cgtgctcgcc ccttacgccg gcgcttccaa
      601 gtaccgcctg cagggccgcc gggtagtcga gggccagcgg ctgacccagg cggccagcga
      661 cctcttcctc ggctggatcc gcgcgaaggg accggatgga aacgaacggg acttctacat
      721 tcgccagctc tgggacggaa agctctcgat tgacatcggc agcctcgatg agctgggtct
      781 gacggaatac gtccgggcct gcggcggcac cctggccctg gcccatgccc ggaccggtga
      841 ccgggtcggg atcgccgact acctcgggaa gaacgacagc ttcgaccagg cgatcgccga
      901 tttcagcctc agctacgcga agcagaacca ggaagacttc aaggcgttca ccgccgagat
      961 cgaggccgga aggatcgacg ccaccccggg gatctgatcc cggcagcgaa tacgatcctg
     1021 ctgtgaagtc ccgtcccttc ttccggctgc tgatccttgt cctgctggcc ctcgccctgg
     1081 ttccggccag tgccggtgcc tctgtggccg gggcggcgaa gcggttggac aacctgcgtg
     1141 accagccgca gcgcctccgg cagttcctgc tcgggatgcc gaagggcaca gacctccaca
     1201 cccacctctc cggcgcggtt tacgcggagg cgatgatccg ctggggcgcc gaggacggca
     1261 agtgcgtcga cccggtcacc ttcgtctcga ccttcccgcc ctgcaccggc agccaggtgc
     1321 cgctgaccac ggccctgacc aacacccagc tctgggacca gatcctcgcg gcctggtcga
     1381 tgcgggagtt ccggcccgac gccttttccg gtcacaacca cttcttcgcg accttcgacc
     1441 tcttcggttc cgccttcagc ggccggcagg gtgacggcct ggcccaggtg acccagcggg
     1501 cggcttccca gaacgtccag tacgtcgagc cgttgatcac gccccagttc ggcgcaacgc
     1561 tcgcgatcgc aaaccaggtc ggctacatcc cggacttcgc cgagatgcgc gaggcgatgc
     1621 tgaacgccgg gatcac
//
LOCUS       DAOQFI010000549         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_50963_length_1631_cov_2.619289, whole genome shotgun sequence.
ACCESSION   DAOQFI010000549 DAOQFI010000000
VERSION     DAOQFI010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50963_length_1631_cov_2.619289"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLE93_09135"
     CDS             complement(<1..1104)
                     /locus_tag="PLE93_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNH87254.1"
                     /translation="MRSIRVYLAALAALIGGLALIGGMATTASAKPKKLKSAPKVTSK
                     ATYTARGSVGDAYVKNAEEGQTLLLVNANNKIVRTGKADSFGSKVFYDVAPGKTFTVR
                     TPKGKGALGTKPFKVLKPGANPPQSFYDKKPDLKPGLNFVTMRDGIEIAMTVRLPSGK
                     TMDDGPFPTFIEYSGYQAAAPHDLLQSIISQLGGGDGTSDDLAPASSTAVGSVIGPLL
                     DFAVVTVQMRGSGCSGGAFDLFGWPTTYDGYDAVETVAAQDWVQGKVGMGGISFSGIS
                     QLFTAGTRPPHLAAVSPLSVTDDVYYGTGFPGGIFNNGFAFSWITERAEDAEPATEGG
                     QTWAKIMTTTGDPEVDEPLRSEQKQHCIDNQKMR"
     gene            complement(1205..1552)
                     /locus_tag="PLE93_09140"
     CDS             complement(1205..1552)
                     /locus_tag="PLE93_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87255.1"
                     /translation="MVCATWTSCHEDDLPHALGQCAPQRVEVLARDHARAYRYFGRAL
                     LVSILVTRVFVFIESQFAAVFGLALDLVLFAAISELASQDEKTRFRFGGLGQADLAEA
                     GSTPEKPSRSQNP"
BASE COUNT          285 a          501 c          551 g          294 t
ORIGIN      
        1 ccgcatcttc tggttgtcga tgcagtgctg cttctgctcg ctgcgcaggg gctcgtccac
       61 ttccgggtca ccggtcgtgg tcatgatctt ggcccaggtc tggccaccct cggtggccgg
      121 ttccgcatcc tcggcccgct cggtgatcca gctgaacgcg aagccgttgt tgaagatgcc
      181 gcccgggaaa ccggtgccgt agtagacgtc gtcggtcacc gaaaggggtg agacggcggc
      241 caggtgaggc ggccgggtgc cggcggtgaa gagctgcgaa atgccggaga aggaaatacc
      301 gcccatgccg accttgccct ggacccagtc ctgggcggcg acggtctcga ccgcgtcata
      361 gccgtcgtag gtggtcggcc agccgaacag gtcgaatgcg ccgccggaac agccggaacc
      421 gcgcatctgg acggtgacca ctgcgaagtc gagcagcgga ccgatgaccg agccgaccgc
      481 ggtcgaagag gccggagcga ggtcgtcact cgtgccgtcg ccgccaccca gctgggagat
      541 gatcgactga agcaggtcat gcggggccgc ggcctggtag ccggagtact cgatgaaggt
      601 cgggaacgga ccgtcatcca tggtcttgcc ggaaggcagg cggacggtca ttgcgatctc
      661 gatcccgtcg cgcatcgtga cgaagttgag gccgggcttg aggtcgggct tcttgtcgta
      721 gaaggactgc ggcgggttgg cgccgggctt caggaccttg aacggcttgg tgcccagggc
      781 gcccttgccc ttcggggtgc gcacggtgaa agtcttgccc ggagcgacgt cgtagaagac
      841 cttggagccg aacgagtcgg ccttgccggt gcgaacgatc ttgttgttcg cattgaccag
      901 cagaagggtc tggccctcct cggcgttctt gacataggcg tcaccgacgc tgccgcgagc
      961 cgtgtaagtc gccttcgaag ttaccttcgg cgcggacttg agcttcttcg gcttggcact
     1021 ggcggtcgtg gccatcccgc caatcagggc aagccccccg atcaatgcgg ccagagcggc
     1081 cagatacacc cgaatactgc gcatgctgcg ttcctccatc cctaatttca ctttgcctgg
     1141 aggcgcctca tgtcccccgt tggagtcatt ctcccattcc ctgcccggga aactatacgg
     1201 gaaatcaggg attctgtgaa cgggagggtt tctcaggtgt tgaaccggct tcggccagat
     1261 cggcctgacc cagcccgcca aagcggaatc gggtcttttc gtcctggctg gcaagctcgg
     1321 atatcgccgc gaagaggacc agatccaggg cgaggccgaa gaccgcggcg aactgggact
     1381 cgatgaagac gaagacccgg gtcaccagga tcgaaacgag cagggcccgg ccgaagtaac
     1441 ggtaggcccg ggcgtggtcg cgcgcaagga cttcgactcg ctgcggagca cactggccaa
     1501 gggcatgcgg cagatcatct tcgtggcagg aagtccacgt cgcgcagacc acccatgcgg
     1561 tcagctcggt ctcgcccttc gccggtcgtt tcgcttatcg accagggtca gggctccggc
     1621 tggatcggac c
//
LOCUS       DAOQFI010000550         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_51061_length_1629_cov_3.008259, whole genome shotgun sequence.
ACCESSION   DAOQFI010000550 DAOQFI010000000
VERSION     DAOQFI010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51061_length_1629_cov_3.008259"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLE93_09145"
     CDS             complement(<1..654)
                     /locus_tag="PLE93_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNH87256.1"
                     /translation="MDLSGTTVALDGLGVESGFDVLNEGIRLAAGDGIRLRVFGPADA
                     IEVSGLPGVEVIDCTDQITNDDDPVPSVRSRPDSSIVRAVADVGAGNSESVVSLGSTG
                     ATMAASTFGLKRLKGVKRPALAAQVPLPGRTVLFLDIGANVEVRAQHLVQFAWLGAAF
                     SKSVLGVERPEVGLLSVGEEAGKGREEVVEAHAALVGAGEFDFIGNVEGRDLPAGNAD
                     "
     gene            complement(664..873)
                     /gene="rpmF"
                     /locus_tag="PLE93_09150"
     CDS             complement(664..873)
                     /gene="rpmF"
                     /locus_tag="PLE93_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNH87257.1"
                     /translation="MAVPKKRTSRTRRDKRRATHKAAKARVNHCPHCHHPRLPHRVCA
                     NCGQYKGREVVAPKEQPFGTVTPDA"
     gene            complement(942..1511)
                     /locus_tag="PLE93_09155"
     CDS             complement(942..1511)
                     /locus_tag="PLE93_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNH87258.1"
                     /translation="MLIEKVDLDALGLSYGDATRIQGEVAPARVSLGGQDYEPVPAEP
                     AFLLEVSRTSAGYALRLRFEVGLSGPCFRCLEDATASVSVDAREVDQPVEPIPIQGSG
                     RIQKDDEDEDESSAAELTSPYVEEGTLDLAAWTHDALILSLPEQILCQPDCLGLCPVC
                     GESLNGADPAAHDHGQDIDPRWAKLRDLK"
BASE COUNT          275 a          540 c          537 g          277 t
ORIGIN      
        1 gtcggcgttc ccggccggca ggtcgcggcc ttcgacgttg ccgatgaagt cgaattcccc
       61 ggctccgacc agggcggcat gagcctcgac gacttcctcc cggcccttgc ccgcctcttc
      121 gcccaccgaa agcagcccga cttcgggccg ctccacaccg agcaccgact tcgaaaacgc
      181 ggcgcccagc caggcgaact gcaccaggtg ctgggcccta acctcgacgt tcgcgccgat
      241 gtcgagaaag agaacggtcc gtcccggcag cggaacctgc gcggccaggg ccggtcgttt
      301 gacccccttc agccgtttca ggccgaaggt tgacgccgcc atcgtcgccc cggttgatcc
      361 gaggctgacg accgattccg aattcccggc acctacatcg gccaccgccc ggacgatcga
      421 ggagtcggga cgcgaccgca ccgaggggac gggatcgtcg tcgttggtga tctggtcggt
      481 gcagtcgatc acctcgaccc ccggcaaacc ggaaacctcg atcgcgtcgg ccggcccgaa
      541 aaccctcagc cggattccgt ctccggcggc aagccggatt ccttcgttca ggacatcgaa
      601 gccgctttcg acgccgaggc cgtcaagcgc gaccgtagtc ccgctcaggt ccaagagttc
      661 aggctaggcg tcgggggtga cggtgccgaa gggctgctct ttgggagcga cgacctcacg
      721 gcccttgtac tggccacagt tcgcgcagac gcgatgcgga aggcgcggat ggtggcaatg
      781 cgggcagtgg ttgacgcggg ccttggcggc cttgtgggta gcgcgccgct tgtcccggcg
      841 ggtccttgag gttcgtttct tcggtacggc cataagacgc agcagtctag aggaatggcg
      901 gctgaagtga catccacccg cccgggatgg ctcccctcct gctatttcag gtcgcgcagc
      961 ttcgcccagc gggggtcgat gtcctggcca tgatcatgcg cggcggggtc ggccccgttc
     1021 agggactctc cgcaaaccgg gcaaagaccg aggcaatcag gctggcagag gatctgctcc
     1081 ggcagggaga ggatcagggc gtcgtgggtc caggcggcca ggtcgagggt gccttcctcg
     1141 acataggggc tggtcagctc cgcggcgctg gattcatctt catcctcgtc gtctttctgg
     1201 atccggcctg aaccctggat cgggatcggt tcgaccggct ggtcgacctc ccttgcgtca
     1261 accgaaacgc tggcggtcgc atcttccagg cagcggaaac agggcccgga aagaccgacc
     1321 tcgaagcgga gcctgagtgc atagccggcg ctggtccgcg agacctcgag caggaaggca
     1381 ggttcggccg gcaccggctc gtagtcctgc ccgccgaggg aaaccctggc cggagccact
     1441 tcaccctgga ttcttgtggc gtctccatag gagaggccga gggcgtcgag atcaaccttt
     1501 tcgatcagca tcccttccca gcatggcaga cgaatacgat gcgctcgatg agccagccgg
     1561 acgaaagcag ctacctcgac tcggtccgcg gccaggtgcc gctttacgac gcgcttcagg
     1621 atgcggcgg
//
LOCUS       DAOQFI010000551         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_51071_length_1629_cov_2.751588, whole genome shotgun sequence.
ACCESSION   DAOQFI010000551 DAOQFI010000000
VERSION     DAOQFI010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51071_length_1629_cov_2.751588"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PLE93_09160"
     CDS             complement(<1..1386)
                     /locus_tag="PLE93_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH87259.1"
                     /translation="MGFELTRRDLGKAALAGAAVAGLGGAAGLAGCAREGGGNGSGAS
                     KQEVIDGLPETGIPRPVDYIRTDWSHDQLAFCSYSCLAPTPYGSEIRRMLAEPVGRIA
                     FAGEATARIPATVHGAIQAGRDAGKYAAGQLRAGARVAVVGAGAAGLSCAGELERLGL
                     KPVVLEASDRVGGRVREADLDGSPVELGASWIHGVDGNPLTELAGEAGVGLHPFRYQL
                     DFPVSGQQALGAGGEHRYWQAVNSFGPRSVRHPDVPLEAILPRRRGPGLEWAIQDEVA
                     QEYGADPDQLAALATWEGNWLPGGDALLDGPYSQVIGSLSGDAGIRFGFEVRKISQRP
                     GEVRLTGAEDNDEVAADAAVVTVPIGVLKAERIEFDPPLPARTRTAIDGLGAGLLDKV
                     WLAFEEPFWDENAEGFSWIDPGRPGRWGAWVNAVPVTGEPFLLTLSGGRDAHRLSRQS
                     DLEIISGTTAAI"
     gene            complement(1462..1534)
                     /locus_tag="PLE93_09165"
     tRNA            complement(1462..1534)
                     /locus_tag="PLE93_09165"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1498..1500),aa:Glu,seq:ctc)
BASE COUNT          255 a          605 c          508 g          261 t
ORIGIN      
        1 gatcgcggcc gtcgtgccgc tgatgatttc gagatcgctc tgacgggaaa gccggtgggc
       61 gtccctgccg ccggagaggg tgagcaggaa cggctctccg gtgactggca ccgcgttcac
      121 ccaggcaccc cagcgtcccg gtctccccgg gtcgatccag ctgaaacctt cggcgttttc
      181 atcccagaac ggttcctcga aggccagcca gaccttgtcg agcaaacccg ccccgagtcc
      241 gtcgatcgcg gtacgggtcc gggccggcag tggcgggtcg aactcgatcc tttccgcctt
      301 cagtaccccg atcggaaccg tgaccacggc cgcgtcggcc gcaacttcat cgttgtcctc
      361 tgccccggtc agccggacct cccccggccg ctggctgatc ttccgcacct cgaacccgaa
      421 tcggatcccg gcgtcaccgg acaggcttcc gatcacctgc gagtagggcc cgtccagcaa
      481 ggcgtcacct ccgggcagcc agttgccttc ccaggtcgcg agcgccgcca gctgatccgg
      541 gtcggccccg tattcctggg cgacctcgtc ctggatcgcc cactcgagcc cgggtccgcg
      601 ccgccgcgga aggattgcct cgagcggaac gtccggatgg cggaccgagc ggggaccgaa
      661 ggaattgact gcctgccagt agcgatgctc gccccccgct ccgagcgcct gctgccccga
      721 caccgggaaa tcgagctggt acctgaacgg gtgaagcccg acaccggcct ccccggcgag
      781 ttcggtcagc gggtttccgt ccacgccgtg gatccacgag gcaccaagtt caaccggcga
      841 accgtcgagg tcggcctccc tgacccttcc tccgacccgg tccgaagctt caagcaccac
      901 cggcttcagg ccgaggcgtt cgagttcacc ggcgcaggag aggccggccg ccccggcccc
      961 gaccaccgcc acccgcgccc cggccctgag ctgcccggcc gcgtactttc ctgcgtccct
     1021 tcccgcctgg attgccccgt gaaccgtggc cggaatcctc gccgtggctt ccccggcaaa
     1081 ggcgatcctg ccgaccggct cggccagcat ccttctgatc tccgaaccgt agggagtcgg
     1141 ggcgaggcag gagtaggagc agaaggccag ctggtcatgc gaccagtcgg tccggatgta
     1201 gtcgaccggc ctggggatcc cggtttccgg aagaccgtca atcacctcct gcttcgaggc
     1261 tccggatccg ttgccaccgc cttcccgggc acaaccggcc agtccggccg ctcctccgag
     1321 cccggccaca gcggcgcctg cgagggccgc cttgcccagg tcccgtctgg tcaactcgaa
     1381 tcccacgctc cgattgtcgc cgatgtcatg ccgatccggt ctgaaaacgg gaaaacccgc
     1441 cggcgggcgg gctttcggag gtaccgctac ggggattcga accccggttt ccggactgag
     1501 aatccggcgt cctagtcccc tagacgatag cggcgcgacg cgcgagtcta gcgaagctcc
     1561 agcgacccgc caaacggggc cgggtgcgcc aacgagcgtg caatcgctgt tctctgagga
     1621 cagctattg
//
LOCUS       DAOQFI010000552         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_51771_length_1618_cov_2.169546, whole genome shotgun sequence.
ACCESSION   DAOQFI010000552 DAOQFI010000000
VERSION     DAOQFI010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51771_length_1618_cov_2.169546"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLE93_09170"
     CDS             complement(<1..400)
                     /locus_tag="PLE93_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNH87260.1"
                     /translation="MSRDHRKPSRYNQQFFRQWGGPGPLFFGLLALIIVLLGLYLAFT
                     KSIPFTSPGYQVQATFRNAVNIAIKSPVRIAGVNVGKVTDMEQKGDNTVVTFSVNDDG
                     RPIREDASAQIRPRLFLEGNWFIDLDPGTPD"
     gene            complement(397..>1618)
                     /locus_tag="PLE93_09175"
     CDS             complement(397..>1618)
                     /locus_tag="PLE93_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87261.1"
                     /translation="TMKLDKSVEPIPEDSTVLVRQRSAMGLKYLLLTPGKSSQGLEPG
                     GTIPESQADEPVDMDQWFNMFQEPVRKAIQKNLAEYGGGFAARGSDINQLIADLPPLL
                     RLAEPVAKNLSSKQTDLRGFVRGLSQAAAEVAPVAETQADMFVQLDRTFIALAEVARP
                     YIQDSITEGVPTELAVIRNGPRIRPFLYTSADFMKALKPGAKALGESAPIVSRSFDIG
                     IPVLRSSPQLYDELAPTARSLRRFGESTSNNEGLDMLISTNEILTPLLRQVTPSQNTC
                     NYLNLLLANVASATGGHNSEGNAVRAVSVLPQIGVNAEGNPASGMANGGTIVPPPDDP
                     TNKNYLHSNPYPNTAAPGQSPRECEPGNIPEGWFIPGGGTTWVNALQVGNLDGDQGLI
                     TQDQTQAQLNWRTK"
BASE COUNT          269 a          471 c          570 g          308 t
ORIGIN      
        1 aatccggggt gccgggatcg aggtcgatga accagttacc ttcgaggaag agacggggac
       61 ggatctgggc cgaagcgtcc tcgcggatcg gccggccatc atcgttgacg gagaaggtga
      121 ccacggtgtt gtcacccttc tgttccatgt cggtcacctt gccgacgttg acgccggcga
      181 tgcgaaccgg ggacttgatc gcgatgttta cagcgttacg gaaggtcgcc tggacctggt
      241 agccggggct tgtgaacggg atcgacttgg tgaaggcgag gtaaaggccg agcaggacga
      301 tgatcagcgc gagcaggccg aagaacagcg gaccgggacc gccccactgg cggaagaact
      361 gctggttgta gcggctgggc ttgcggtggt cgcggctcat ttggtccgcc agttcagctg
      421 cgcctgggtc tggtcctggg tgatcaggcc ctgatcgccg tcgaggtttc cgacctgaag
      481 cgcgttgacc caggtcgtgc cgccgcccgg gatgaaccag ccctcgggga tgttgcccgg
      541 ctcacattcg cgcggtgact ggcccggggc tgcggtgttc gggtacgggt tcgagtgcag
      601 gtagttcttg ttggtcgggt cgtcgggcgg gggaacgatc gtgccaccat ttgccatgcc
      661 ggatgcggga ttgccttcgg cattgacgcc gatctggggc agcacgctga ccgcccgcac
      721 cgcgttgccc tcgctgttgt ggccgccggt cgccgaggcc acgttggcga gcagaaggtt
      781 gaggtagtta caggtgttct gcgagggcgt cacctgacgc agcagcgggg tgaggatctc
      841 gttggtgctg atcagcatgt cgaggccctc gttgttcgag gtgctctcgc cgaatcgcct
      901 cagcgaacgg gcggtcgggg ccagctcgtc atagagctgc ggtgaggagc gcagcaccgg
      961 gatgccgatg tcaaacgacc tggaaacgat cggggcactc tcgccgagtg ccttcgcccc
     1021 cggcttcaac gccttcatga agtcggccga ggtatagagg aagggacgga tccggggccc
     1081 gttgcggatc accgcgagct cggtcgggac accttcagta atcgaatcct ggatgtaggg
     1141 gcgggccact tcggccagcg cgatgaaagt gcgatcgagc tggacgaaca tgtcggcctg
     1201 ggtctcggcc accggagcca cctcggctgc cgcctgcgaa agaccgcgca cgaagccccg
     1261 aaggtcggtc tgcttcgagg aaagattctt cgccactggc tcggccagcc tgagcagcgg
     1321 cggcaggtcg gcgatcagct ggttgatgtc cgagccacgg gcggcgaaac cgccaccgta
     1381 ctcggccagg ttcttctgga ttgccttgcg gaccggttcc tggaacatgt tgaaccactg
     1441 gtccatgtca accggttcat ccgcctggga ttcgggaatc gtgccacccg gctcaagacc
     1501 ctgactggac ttgcccggcg tgagcagcag gtacttcagg cccatggccg agcgctggcg
     1561 gaccagtacg gttgaatcct cggggatcgg ctcgacgctc ttgtccagtt tcatcgtc
//
LOCUS       DAOQFI010000553         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_51917_length_1615_cov_4.516667, whole genome shotgun sequence.
ACCESSION   DAOQFI010000553 DAOQFI010000000
VERSION     DAOQFI010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51917_length_1615_cov_4.516667"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="PLE93_09180"
     CDS             complement(<1..1426)
                     /locus_tag="PLE93_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87262.1"
                     /translation="MNHSSATLRRPVLLAIALVTALTMLAAWSASSRAAVICVPDAGV
                     SPDCQTSFATIQPALDSAKNSGGNDEIRLGTGSFSGDLSYDNTTAGNTLDIIGSGQNP
                     GQTVLNMVDTAGNNEGFHFTSVPGSTIRNLTYRIPIAADGSSDVGLNLNGVTADGIVV
                     DGPTASQVRAIACSTCNLTGSTIDLPIENGTIGVDQTGGPAYVGDSVIKADYGVRHSS
                     PGLTTTVERSRITAHTGASTDGGNLEIFNTLILLDDQPNAVGINLYNPNEGTYAIGAT
                     LDHLTIISGGGQHSIGIRAIADSESSGEGESVDATLTNSVIHGFNGTGEHTLDLLADK
                     GRTVTFHSSYSAYDNARTSNVTGSEGGTVDYELSPGHLDLEGGVSFVDPQSGDYTPSL
                     LGGLLDGGDPIGPLTGITDINGNPRACHGTDEGIIRQDIGAFEARTVVSSEPDDCTYP
                     VTSILAKPPASTTAKTASFTLGTDK"
BASE COUNT          252 a          440 c          572 g          351 t
ORIGIN      
        1 tcttgtcggt gcccagggtg aaggaggccg tcttcgcggt ggtgcttgcg ggaggcttgg
       61 caaggatcga agtcaccggg taggtgcagt cgtcgggctc ggagctgaca accgtccggg
      121 cctcgaaggc gccaatgtcc tgacggatga tgccttcgtc ggtcccgtgg caggcacgag
      181 ggttgccgtt gatgtcggtg atgccggtaa gggggccgat cgggtcgccg ccatcgagga
      241 ggccgccgag cagggaggga gtgtaatcgc cactctgcgg atcgacgaag gaaacgccgc
      301 cttcgaggtc gaggtggccc ggcgaaagct cgtagtcgac cgtgccgccc tccgatccgg
      361 tcacgttcga tgtcctggcg ttgtcgtagg ccgagtaact cgaatggaaa gtgaccgtcc
      421 gtcctttatc cgccaaaaga tcaagcgtgt gctcgccggt tccgttgaag ccgtggatca
      481 ccgagttggt cagggtggca tccacacttt cgccttcgcc gctcgactcg ctgtcggcga
      541 tcgcgcggat gccgatgctg tgctggccgc ccccgctgat gatcgtcagg tggtcgaggg
      601 tggcgccgat cgcataggtc ccttcgttgg ggttgtagag gttgatcccg accgcgttcg
      661 gctgatcgtc gagaaggatc agggtattga agatctcgag gttgccaccg tcggttgaag
      721 ccccggtgtg cgccgtgatt cggcttcttt cgacggttgt ggttagaccc ggggaactgt
      781 gcctgacccc atagtcggct ttgatcacgg agtcgccgac atacgccggt cctccggtct
      841 ggtcgacgcc gatagtgccg ttctcgatcg ggaggtcgat ggtcgagccg gtcaggttgc
      901 aggtgctgca tgcgatcgcc cggacctggg aggcggtcgg gccatcgacg acgattccgt
      961 cggcggtcac gccgttgagg ttgagaccga catccgagct gccatcggca gcgatgggaa
     1021 ttctgtaagt caggttcctg atcgtcgagc cggggaccga agtgaagtgg aagccttcgt
     1081 tgttgccggc cgtgtcaacc atgttcagga cggtctggcc ggggttctgc ccggagccga
     1141 tgatgtcgag cgtgttgccg gcagtggtgt tgtcgtagga gaggtctccg gaaaagctgc
     1201 cggtgcccag tcggatttca tcgttgccgc cgctgttctt ggctgagtcg agggctggct
     1261 ggatggttgc gaagctcgtc tggcaatccg ggctgacccc tgcgtccggg acgcagatca
     1321 ccgcggccct ggagctggcg ctccaggcgg ccagcatggt gagcgcagtg accagggcga
     1381 ttgcgaggag aacgggacga cgaagagtgg ctgaagaatg gttcattgga atggagatag
     1441 gaacctgtgt ttatttgcgc ttgacggcga ctgtctttac cttcgacctg ttgcctgccc
     1501 ggtctgtggc ctgaatgctg atcgagttcc tgcccttgcg gagcttcggg gtcttccagg
     1561 gtgacttgca gcttttccac ttgcccttgc cgaggcggca cttgaaggtc gagcc
//
LOCUS       DAOQFI010000554         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_51920_length_1615_cov_4.032051, whole genome shotgun sequence.
ACCESSION   DAOQFI010000554 DAOQFI010000000
VERSION     DAOQFI010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51920_length_1615_cov_4.032051"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PLE93_09185"
     CDS             <1..934
                     /locus_tag="PLE93_09185"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNH87263.1"
                     /translation="AIITDSGGFQVFSLAHGGVAEEIKGSGRKMYGLAKTLGIEEEGV
                     RFRSYKDGSELMLSPEESMRVQAALASDIALVFDECTPFHADREYTARSTERTHRWLK
                     RCLDWHEANGPAGQAVFGIIQGGTHADLRRESCEAVSSSAVDGISIGGTLGRDKEEMA
                     EVLGFTVPNLPVEAPKHLLGIGEVDDLMRGVALGLDVFDCAVPTRLARHGMALAPLPE
                     KRFRIDVRKPREIGNREPLVPGCPCAACERYDRDYLNYISKSEELTAVRLLVDHNLTY
                     MERLMRGAREAISAGTYAAYSERILGGLAPWEAG"
     gene            948..>1615
                     /locus_tag="PLE93_09190"
     CDS             948..>1615
                     /locus_tag="PLE93_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH87264.1"
                     /translation="MPETLIRLEGVTKKFGATVALDDLSLAVPEGSVCGLLGPNGSGK
                     TTAIRILLGLAGMNSGTASLLGARPGGPGFTEAVRQTGSLIEGPALYGRATARQNMQI
                     QAATLGLADAGAEIDDLLELVDLGDRADSNARTFSLGMKQRLGLAISLLGKPRLVLLD
                     EPTNGLDPSGIVEIRELIKQLPERGVTVLVSSHLLSEVQLMCDRVTIINKGRLVADGT
                     MEEIL"
BASE COUNT          267 a          541 c          543 g          264 t
ORIGIN      
        1 ggcgatcatc accgactcgg gcggcttcca ggtcttctcg ctggcccacg gcggggtggc
       61 cgaggagatc aagggctcgg gacgcaagat gtacgggctc gccaaaaccc tggggatcga
      121 ggaggaggga gtgcgattcc gttcctacaa ggacggctcc gagttgatgc tttcaccgga
      181 ggagtcgatg cgggtccagg ccgcgctcgc ttccgacatc gccctggtct tcgatgaatg
      241 cacccccttc cacgccgacc gtgagtacac cgcccgctcg accgagcgga cgcaccgctg
      301 gctgaaacgc tgcctcgact ggcacgaagc caatggcccg gccgggcagg cggtcttcgg
      361 gatcatccag ggaggcaccc acgccgacct gcgcagggag tcctgcgagg cggtctcctc
      421 atctgcggtt gacgggatct cgatcggcgg caccctcggc cgcgacaagg aagagatggc
      481 cgaagtgctc ggtttcacgg tgccgaacct gccggtcgag gctcccaagc acctgctcgg
      541 gatcggcgaa gttgatgacc tgatgcgagg cgtggcgctc ggcctcgatg tcttcgactg
      601 tgccgttccg acccggctcg cccgccacgg catggccctg gcgccgctgc cggagaagcg
      661 cttccggatc gatgtccgca agccacgcga gatcggcaac cgcgagccgc tggtccccgg
      721 ctgcccctgc gcggcctgtg agcgctacga ccgggactac ctcaactaca tctcgaagtc
      781 ggaagagctg accgccgtcc ggctgctggt cgaccacaac ctgacctaca tggaacggct
      841 gatgcgtggt gcccgcgagg cgatttcggc cggaacctac gctgcctaca gtgagcggat
      901 cctgggcggt ctggcgccct gggaagccgg ttaggattcc gcccgcgatg cccgagacgc
      961 taatccggct ggaaggagtg accaagaagt tcggggccac cgtcgccctg gacgacctct
     1021 cccttgcggt gcccgagggg tcggtctgcg ggctgctcgg tccaaacggt tccggcaaga
     1081 cgaccgcgat caggatcctg ctcggcctgg ccgggatgaa cagcggaacc gcgagcctgc
     1141 tcggagcccg gcccggcggc cctggtttca ccgaagcggt tcgccagacg ggctcgctga
     1201 tcgagggccc ggcgctctac ggccgtgcca ccgcccggca gaacatgcag atccaggccg
     1261 cgacgctcgg gctcgcggat gccggggccg agatcgacga cctgctggaa ctggtcgacc
     1321 tgggcgatcg ggccgactcc aacgcccgga ccttttcgct gggcatgaag cagcgcctcg
     1381 gtctggcgat ctccctgctc ggcaagcccc ggctggtcct gctcgatgag ccgaccaacg
     1441 gtctcgaccc ttcggggatc gtcgagatca gggaattgat caagcaactt cccgagcgcg
     1501 gcgtgaccgt gctggtctct tcccatctgc tctccgaggt ccagctgatg tgcgaccggg
     1561 tcacgatcat caacaagggc aggctggttg ctgacggaac catggaggag atcct
//
LOCUS       DAOQFI010000555         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_51991_length_1614_cov_3.706863, whole genome shotgun sequence.
ACCESSION   DAOQFI010000555 DAOQFI010000000
VERSION     DAOQFI010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51991_length_1614_cov_3.706863"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PLE93_09195"
     CDS             <1..977
                     /locus_tag="PLE93_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNH87265.1"
                     /translation="IRAITPAGEWQSRRLPGSGAGPSPDRMLLGSEGILGVVTEAWMK
                     VRPRPDFKATATVEFPPGDAAFSEAARGAVRLIAQSRLMPSNLRLIDSSEAATTMAGD
                     GSATLLIIGFESHGQPVEDRMDQAVAIAEGEGGRVASRSSSTPSDRAGNKGGGGEDEA
                     GADRWRSAFLMAPYMRDTFVACGIIAETFETAITWDRFEEFHRSVIETTRRAVAEVTG
                     APSEGEGAPRVSCRLTHVYPDGAAPYFTVLAPARRGSEVAQWDEIKAAASQAIIENGG
                     TITHHHAVGRDHRPWYDQQRPDQFAAALTAAKSAVDPDHMLNPGVLID"
     gene            complement(1051..1305)
                     /locus_tag="PLE93_09200"
     CDS             complement(1051..1305)
                     /locus_tag="PLE93_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87266.1"
                     /translation="MAAAVAGAKEEAAVPAANAFSQIHEALTDSLTGAGVKKPRASSL
                     ATLAISAIEGAIILSRTEKSTEPLNQTRQELHDIYAAALP"
     gene            complement(1317..>1614)
                     /locus_tag="PLE93_09205"
     CDS             complement(1317..>1614)
                     /locus_tag="PLE93_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH87267.1"
                     /translation="YELEQVVRFCREQGILTCLDCIQAIGATSFDLRQIDADFAVSGA
                     QKWLMGPVGCGILYAQSQHLEALDVPFLGWTSVKYPELFDLGPLQYPPDARISA"
BASE COUNT          267 a          534 c          546 g          267 t
ORIGIN      
        1 cgatccgcgc gatcaccccg gccggcgagt ggcagtcccg ccgcctgccc ggttccggcg
       61 ccgggccttc cccggaccgg atgctgctcg gctccgaggg catcctcgga gtggtaaccg
      121 aggcgtggat gaaggttcgg ccccgaccgg acttcaaggc gaccgcgacg gtcgagttcc
      181 ctcccggaga tgccgccttc tccgaggcgg cccgtggcgc ggtccggttg atcgcccagt
      241 cgcggttgat gccttccaac ctgcgcctga tcgattcctc ggaggccgcg acgacgatgg
      301 ccggtgacgg ttcggcgacc ctgctgatca tcggcttcga atcccacggc cagccggtcg
      361 aagaccgcat ggaccaggcc gtggcgatcg ccgagggcga gggcggccgg gttgcttcgc
      421 gcagttcatc gaccccgtcc gaccgggccg gcaacaaagg gggcggggga gaagacgagg
      481 ccggcgccga ccgctggcgt tcggccttcc tcatggcccc gtatatgaga gacaccttcg
      541 tcgcctgcgg gatcatcgcc gagaccttcg agaccgcgat cacctgggac cgcttcgagg
      601 agttccaccg cagcgtgatc gaaaccaccc ggcgcgccgt cgccgaggtc accggcgccc
      661 cgagcgaagg cgaaggtgcc ccgcgggtca gctgtcgcct cacccacgtc tacccggacg
      721 gcgccgcccc ctacttcacg gtcctcgcgc ctgcccgacg aggcagcgag gtagcccagt
      781 gggacgaaat caaggccgcc gcctcgcagg cgatcatcga aaacggcgga acgatcaccc
      841 accaccacgc ggtgggcagg gaccaccgcc cctggtacga ccagcagcgc cccgaccagt
      901 tcgccgctgc cctcaccgcc gccaagtcag ccgtcgaccc cgaccacatg ctcaacccgg
      961 gcgtcctgat cgattgagtg ggggctgggg aatcgagatg gcaaaaggga gcgctatatg
     1021 gccacttttg ccttctcgat ctaatgtggc tcaggggagc gcggcggcgt agatatcgtg
     1081 gagttcctgg cgggtctggt tgaggggttc ggttgatttc tcggttcggc tgaggatgat
     1141 cgctccctcg attgccgaga ttgcgagggt ggcgagggag ctggcccggg gtttcttcac
     1201 cccggccccg gtgagggaat cggtgagggc ttcgtggatc tggctgaagg cgttggcggc
     1261 ggggacggcg gcctcttcct tggctccggc gaccgcggcg gccatcaggg ggcagcctac
     1321 gcggaaatcc gtgcgtcggg tggatattgc aagggcccaa ggtcaaacag ttcgggatac
     1381 ttgacgctgg tccagcccag aaatggcaca tccagtgcct ccagatgctg gctttgcgca
     1441 tacagaatgc cgcagcccac aggtcccatc agccattttt gggctccgct gactgcaaag
     1501 tcggcgtcga tttgccgcag gtcaaaggag gtcgcaccga tggcctggat gcaatccagg
     1561 caggtcagga tgccctgctc gcgacagaag cggactactt gctccagctc gtaa
//
LOCUS       DAOQFI010000556         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_52259_length_1610_cov_2.927974, whole genome shotgun sequence.
ACCESSION   DAOQFI010000556 DAOQFI010000000
VERSION     DAOQFI010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52259_length_1610_cov_2.927974"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1610)
                     /locus_tag="PLE93_09210"
     CDS             complement(<1..>1610)
                     /locus_tag="PLE93_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87268.1"
                     /translation="KIAGNRIGTNSAGTAAIPNTFEGIKVDAAGSPFGAGILIGGITG
                     LTPGGPCTGDCNLISGNLRHGVAIDDGSQFVSILGNSIVGNQLLGIDLRDLALSGGTP
                     LVNDADDVDGGSNGFQNHPVLQAALNQAGKLRVTGILNSVADRNYRIEIFGNGAADSS
                     GYGEGETFLGAFTVSNAPGQTSVGFAETLSVPVATGNTLVATATVLDPVTGRPASSEF
                     SINSTEGCDQSGDPDPNELTASAGGEAICGQGGADVLKSGVAGDILRGDAGSDSADFS
                     GLPYAVSASLASGLARAIPLASGVADILFTVENLRGSPQDDTLTGDTDTNVILGGAGD
                     DEINPGGGPDSVYGGPDDDTINVVDGLADNLIDCGTGVDTVTADPTATEPASLFVDCE
                     TINRPGDPPPTCETDPDLCPPPTCETDESLCPPEPVVYKCDGIKATIVGTNHDETING
                     TNQRDVIVARAGKDKINPKKGNDLICAGDGVDSVSGSGGNDRALGQDGADNLKGGDGN
                     DKLLGGAKDDKLYGHAHGDILKGGEGSDR"
BASE COUNT          223 a          539 c          539 g          309 t
ORIGIN      
        1 cggtccgacc cttccccgcc cttgaggatg tcaccgtggg cgtggccgta gagcttgtcg
       61 tccttggcgc cgccgagcag tttgtcgttg ccgtcgccgc ccttgaggtt gtcggccccg
      121 tcctggccca gggcccggtc gttgccgccg ctgccggaaa ccgagtcgac cccgtcaccg
      181 gcgcagatca ggtcattgcc ctttttcggg ttgatcttgt ccttgccggc ccgggccacg
      241 atcacgtccc gctggttggt gccgttgatc gtctcgtcat ggttggtgcc gacgatcgtc
      301 gccttgattc cgtcgcattt gtagacgacc ggttccggcg gacaaaggct ttcgtcggtc
      361 tcgcaggtcg gcggcgggca gaggtcgggg tcggtctcac aggtcggcgg cgggtcgccc
      421 ggtcggttga tcgtctcgca atcgacgaac aggctggccg gttcggtcgc ggtcgggtct
      481 gcggtcaccg tgtcaactcc ggttccgcaa tcgatcaggt tgtcggccag cccgtcaacc
      541 acgttgatcg tgtcgtcgtc cgggcccccg taaacggagt cggggccgcc accggggttg
      601 atctcgtcgt ctcccgcacc gccgagaatc acgttcgtgt cggtgtctcc ggtcagggtg
      661 tcatcctgcg ggctgccgcg caggttctcg accgtgaaca ggatgtcggc aaccccggaa
      721 gcgagcggaa tggctctcgc caggccagat gccaggctgg ccgacaccgc gtacggcagg
      781 cctgaaaagt cggccgagtc cgagcccgcg tcgccccgca ggatgtcccc cgcgactccg
      841 ctcttcagca cgtcggcacc gccctgcccg cagattgctt ctccgccggc cgaggccgtc
      901 agttcgttcg ggtcgggatc cccgctctgg tcgcaaccct cggttgaatt gatcgagaac
      961 tcggagctgg caggccggcc ggtcaccggg tcgaggacgg ttgccgtggc caccagagtg
     1021 ttcccggtgg cgaccggcac cgacagcgtc tcggcgaacc ccacgctggt ctggccgggc
     1081 gcgttcgaca cggtgaaggc accgagaaag gtctcccctt cgccgtatcc gctggagtcg
     1141 gccgccccgt tgccgaagat ctcgatccgg tagttccggt cggcgacgga gttgaggatg
     1201 ccggtcaccc gcagcttccc ggcctggttc agggcggcct ggaggacggg atggttctgg
     1261 aaaccgttgc ttccgccatc aacgtcatcg gcatcgttga ccagcggcgt acccccggaa
     1321 agcgccaggt cccgaaggtc gatgccgagc agctggttgc cgacgatcga gttccccagg
     1381 atcgagacga actggctgcc gtcgtcaatc gccacgccgt gccggagatt gcccgagatc
     1441 aggttgcagt caccggtgca ggggcccccg ggtgtcaggc cggtgattcc gccgatcaga
     1501 atccccgccc cgaacggact gccggcagcg tcaaccttga tgccttcaaa agtgttgggg
     1561 atggcagcgg tcccggcgct gttcgtgccg atccggttgc cggcaatctt
//
LOCUS       DAOQFI010000557         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_52330_length_1609_cov_2.749678, whole genome shotgun sequence.
ACCESSION   DAOQFI010000557 DAOQFI010000000
VERSION     DAOQFI010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52330_length_1609_cov_2.749678"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLE93_09215"
     CDS             <1..745
                     /locus_tag="PLE93_09215"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH87269.1"
                     /translation="YSTNPNQVLAWPPDGFTLSWFPKAIQDQAARDALWNSVKVGAAA
                     TAVALVLGTLASLAVARHKFFGRESITFLVLIPIALPGIVTGIALNATFTQVFDMKLT
                     LFTVIVAHATFCIVIVFNNAAARLRRMSTTLEDASADLGATPWATFRRVTLPNMRSAL
                     VAGGLLAFALSFDEIVVTNFTIGAGDQTLPIYIFNTLFRPNQPGKVYAVAVIAILLSM
                     IPVYIANRLTKEESVTASGAGAGTPIPEP"
     gene            887..1147
                     /locus_tag="PLE93_09220"
     CDS             887..1147
                     /locus_tag="PLE93_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87270.1"
                     /translation="MERIESRVAKVAKSDKTPKAAAAVVLSGSLLGGADNVQAVTLTA
                     ATLAVAEMARSRFGVKGLRSSGTRSSDKKIGGRIGSVAGRTP"
     gene            1206..1502
                     /locus_tag="PLE93_09225"
     CDS             1206..1502
                     /locus_tag="PLE93_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87271.1"
                     /translation="MEVVSLFHSCVYTGLLICAFLLGKPQPVTFILGLSHGLLWIGMS
                     IASILAVHYRVINLRLALAITLLGCIAPFFGTIEFLLQSHRRKLAEADAEAVAS"
BASE COUNT          247 a          577 c          483 g          302 t
ORIGIN      
        1 ctactcgacc aacccgaacc aggttctcgc ctggccgccg gatggcttca cgcttagctg
       61 gttccccaag gcgatccagg accaggccgc ccgggacgcg ctctggaact cggtcaaggt
      121 cggcgccgct gccacggccg tcgcattggt cctgggcacg ctggcttcgc tggcggtcgc
      181 ccgccacaag ttcttcggcc gggaatcgat caccttcctg gtcctgatcc cgatcgccct
      241 gcccggcatc gtcaccggta tcgccctgaa cgcgaccttc acccaggtct tcgacatgaa
      301 gctgaccctg ttcactgtga tcgtcgccca cgcgaccttc tgcatcgtga tcgtcttcaa
      361 caacgcggcg gcgcgcctgc ggcgcatgtc gaccacgctc gaggacgctt ccgccgacct
      421 cggcgccacc ccctgggcca ccttccggcg ggtgacgctg ccgaacatgc gctcggccct
      481 ggttgccggc ggcctgctcg ccttcgccct ctccttcgac gagatcgtgg tcaccaactt
      541 cacgatcggg gccggcgacc agaccttgcc gatctacatc ttcaacaccc tgttccggcc
      601 caaccagccg ggcaaggtct acgcggtggc ggtgatcgcg atcctgctct cgatgatccc
      661 ggtctacatc gccaaccggc tgaccaagga ggagtcggtc accgcctccg gtgcgggagc
      721 cggaacgccg attcccgagc cgtaacgggc tttgcgaggt cgaaccggag ggcgccggga
      781 tgcgtctggt cggcgatatt cgccgaccaa gtgcatctcg cggccggcgc cggcggattc
      841 acaggatttc aacttcgcgc cctagttccg gtcgtacctt gatgccatgg aacggatcga
      901 aagcagagtg gcgaaggtcg ccaaatcgga caagaccccc aaggcggcag ctgcggtggt
      961 gctctccgga tctctcctcg gcggagccga caacgtccag gcggtcaccc tgaccgcggc
     1021 aaccctggcc gttgccgaaa tggcacgaag ccgcttcggc gtgaaaggct tgcgctcgag
     1081 tggcacccgc agctccgaca agaagatcgg cggccgaatc ggatcggtcg ccggccgcac
     1141 cccctgatcc tctctccccg agggggttcg gcacccgttt cagaaggctg gtcactttcc
     1201 atcggatgga agtggtcagc cttttccatt cctgcgttta cacgggcctt ttgatctgcg
     1261 cctttttgct cggcaagccg cagccggtca ctttcatcct cggcctctcc cacggcctgc
     1321 tctggatcgg catgtcgatc gcctcgatcc tcgcggtcca ctaccgggtg atcaacctgc
     1381 ggctcgccct cgcgatcacc ctgctcggct gcatcgcccc cttcttcggc acgatcgagt
     1441 tcctgcttca gagccaccgc cggaaactgg ctgaagccga tgccgaggcg gtggcctctt
     1501 gagcgggcat cttcgcctgg tcgtcaccaa cgcgatgctg gacggcgagg cggtctcgct
     1561 gaccgcgctt gacggagtga tcaccgcgat cgggcccgac gccggcggg
//
LOCUS       DAOQFI010000558         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_52755_length_1602_cov_2.942469, whole genome shotgun sequence.
ACCESSION   DAOQFI010000558 DAOQFI010000000
VERSION     DAOQFI010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52755_length_1602_cov_2.942469"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1602)
                     /locus_tag="PLE93_09230"
     CDS             complement(<1..>1602)
                     /locus_tag="PLE93_09230"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNH87272.1"
                     /translation="GITEVYNRQIERLSSLPDTPAGQAERSRLIALAAQVISKYGLVE
                     APTLEDPTFVRRVVFSEGGVRAGPKPKLNYIFPSPDAAQIVIRLRSDLTGAERTRAIG
                     LIKEAASDPSIVLRRSQLVVSGSPVVFNGLNDELPVRVLILAAVAVLLMSLALWFTFG
                     SIWRLLPLALALGGMAVAAGLLRLLGGEFSLASLGAAPILIGLTVDYAVQLQARFDET
                     PPLPPEQAAREAAGLGVPMIATACVATAAGFGTLIFSSLPLVSQFGLLLGGGVLICFA
                     FTFLAGFALLGLRGRRRPEPGRGRLIGRVRNLVKSVLATAIMAPGRVLALAVLLGAIG
                     WAVSTQAEVRTEVGQLLPSRAPVVRDLLDVEKTTGASGEIDLIVRAPDVTDPAVVVWT
                     DEVRNTILNQSGYDTNDPSCEGAELCPGPAIPDFVDPTARGLTSADIRGVLKALPASD
                     RKAMIAGGLTGKGDPTVAKIAFALRAGSVDGQQEVIDRMQSAISDSRGGEGPPPGVSA
                     EVTGLPVVVASSANDLADSRYLLVAA"
BASE COUNT          263 a          550 c          574 g          215 t
ORIGIN      
        1 ggcggcaacc agcagatagc gggagtcggc caggtcgttg gctgacgagg ccaccacgac
       61 cgggagtccg gtcacttcgg cgctcacgcc gggcggcggg ccctcgcccc cgcgactgtc
      121 ggagatcgcg ctctgcatgc ggtcgatcac ttcctgctgc ccgtcaaccg aaccggcccg
      181 gagcgcgaag gcgatcttcg ccacggtcgg atcccctttg ccggtcaggc cgccggcaat
      241 catcgccttg cggtcgctgg ccgggagcgc tttgaggacc ccccggatgt cggccgaagt
      301 cagcccgcgc gcggtcgggt cgacgaagtc cggaatcgcc ggcccgggac agagttcggc
      361 cccttcgcag gaagggtcgt tcgtgtcgta accggactgg ttgaggattg tgttcctgac
      421 ctcatcggtc cagacgacca ccgccggatc ggtcacgtcg ggcgcccgga cgatcaggtc
      481 gatctcaccg gaggctccgg ttgtcttttc cacgtcgagc agatcccgga cgaccggggc
      541 gcgggaaggc agaagctggc cgacctcggt ccggacctcg gcctgggtgc tgaccgccca
      601 gccgatagcg ccgagcagca cggccagggc gagcaccctg cccggtgcca tgatcgcggt
      661 tgccagaacc gacttgacga ggttccgcac ccggccgatg agccggcccc ggcccggttc
      721 ggggcggcgc cggccgcgca gccccagcag ggcaaatccg gcgaggaagg tgaaggcgaa
      781 acagatcagc accccgccgc cgagaagcag gccgaactgg ctgaccagcg gcagggagga
      841 gaagatcagc gtcccgaagc cggccgccgt cgcaacgcag gcggtggcga tcatcggcac
      901 cccgaggccg gccgcttcgc gggcggcctg ctccggtggc agcggcggtg tctcgtcgaa
      961 gcgggcctgg agctgcaccg cgtaatcgac cgtcaacccg atcaggatcg gggccgcgcc
     1021 cagcgaggcg agggagaact cgccgccgag gagcctgagc agaccggccg cgacggccat
     1081 tccaccgagg gcgagcgcga gcggcagaag gcgccagatc gagccgaagg tgaaccagag
     1141 cgccaggctc atcaggagca ccgccacggc ggccaggatc agcaccctga ccggcagctc
     1201 gtcgttgagg ccgttgaaga ccaccggcga gccggagacc acgagctgcg agcggcgaag
     1261 cacgatcgaa gggtcgcttg ccgcttcctt gatcagaccg atcgcccggg tccgctccgc
     1321 cccggtcagg tcgcttcgca gccggatcac gatctgggcc gcgtcgggtg aagggaagat
     1381 gtagttcagc ttcggcttcg gcccggcccg gaccccgccc tcgctgaaga cgaccctgcg
     1441 aacgaaggtc gggtcttcga gggtgggtgc ttcaaccagc ccgtacttcg agatgacctg
     1501 ggccgccagg gcgatcaggc ggctgcgctc ggcctgcccg gccggggtgt ccggcaggct
     1561 ggacagccgc tcgatctgcc ggttgtagac ctcggtgatg cc
//
LOCUS       DAOQFI010000559         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_52824_length_1601_cov_4.250970, whole genome shotgun sequence.
ACCESSION   DAOQFI010000559 DAOQFI010000000
VERSION     DAOQFI010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52824_length_1601_cov_4.250970"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="ispH"
                     /locus_tag="PLE93_09235"
     CDS             complement(<1..798)
                     /gene="ispH"
                     /locus_tag="PLE93_09235"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNH87273.1"
                     /translation="MPETPSTGIEKVILAAPRGYCAGVDRAVQTVERALDNHGAPIYV
                     RKEIVHNKHVVEQLAARGAIFVEEETEVPEGEIVVFSAHGVSPAVHERAAARELRTID
                     ATCPLVTKVHVEARKFAAEGYTIIMVGHEGHEEVEGTMGEAPDSIVLVQTVDEVDHLE
                     IEDPDHVALITQTTLSVDETAGIIARLRERFPGIVTAKGEDICYATTNRQIAVKQLAR
                     QCDLVLVIGSTNSSNSNRLVEVAREHGSESFLIDNHTQVQEEWLDGGE"
     gene            849..>1601
                     /locus_tag="PLE93_09240"
     CDS             849..>1601
                     /locus_tag="PLE93_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNH87274.1"
                     /translation="MEPASHELDRLTAIDAQFLTNEHENAHMHIGAVMIFEGDPPTYD
                     EFKAHIGSRLDFVPRYRQKLVYPPYGTGRPLWADDPDFNLNYHLRHTALPRPGGEREL
                     KILASRLFSQALDRSKPLWEMFLVEDLEGDRFAIIIKSHHAMIDGISGVDIGTVIFDV
                     TADAEPIVPKEEWKPKPLPSNLSLLERGAKELATEPVKLGEKAINALRKPSSLAGRVG
                     ESAEGLAEVARALADSAPDVPLNVPIGPHRRIT"
BASE COUNT          271 a          509 c          520 g          301 t
ORIGIN      
        1 ttcccccccg tcgagccact cttcctgaac ctgggtgtgg ttgtcgatca ggaaggattc
       61 ggagccatgc tcgcgcgcga cctcgaccag ccggttcgag ttcgaggagt tggtcgagcc
      121 gatcaccagg accaggtcac actggcgggc cagctgcttg accgcgatct gacggttggt
      181 ggtggcgtaa cagatgtctt cgcccttggc ggtgacgatt ccggggaacc tttcgcgcag
      241 gcgggcgatg atcccggcgg tctcatcgac cgaaagggtg gtctgggtga tcagggcgac
      301 gtggtccgga tcctcgattt caaggtggtc gacctcgtca acggtctgga ccaggacgat
      361 cgagtccggt gcctcgccca tcgtgccttc aacctcttcg tggccctcgt ggccgaccat
      421 gatgatcgtg tagccctcgg ccgcgaactt gcgggcctcg acgtggacct tggtgaccag
      481 cgggcaggtc gcgtcgatcg tgcgcagctc ccgcgccgca gcccgttcgt gaaccgccgg
      541 cgaaacgccg tgggcggaga agacgactat ctcgccttcg ggcacctcgg tttcctcttc
      601 gacgaagatc gctccccgcg ccgctagctg ctcgaccacg tgcttgttgt ggacgatttc
      661 cttgcggacg tagatcgggg caccgtggtt gtcgagcgcg cgctcgaccg tctgcacggc
      721 ccggtcgacg ccggcgcagt agccacgggg cgcggccaga atgactttct ctatcccggt
      781 ggagggtgtt tcgggcattg gacaagggta cccgagtaga gtcttgtgct agtccacgat
      841 ggggagccat ggaacccgcc agccacgaac ttgaccgact cactgcgatt gatgcgcagt
      901 ttctgaccaa cgagcacgaa aacgctcaca tgcacatcgg tgcggtgatg atcttcgaag
      961 gggatccgcc gacctatgac gagttcaagg cccacatcgg ctcgcggctc gatttcgtgc
     1021 ccaggtaccg ccagaagctg gtctatccgc cttacggcac cggccgtccg ctctgggccg
     1081 atgatcctga tttcaacctg aactaccacc tgcgccacac cgccctgccg aggccgggtg
     1141 gcgaacgcga gctgaagatt ctcgcctcgc ggctcttctc gcaggccctg gaccgttcca
     1201 agccgctctg ggaaatgttc ctggtcgagg atctggaggg cgaccggttc gcgatcatca
     1261 tcaagagcca ccacgcgatg atcgacggca tctccggggt tgacatcggc acggtgatct
     1321 ttgacgtcac tgccgacgcc gagccgatcg tccccaagga ggaatggaag ccgaagccgc
     1381 tgccctcgaa tttgagcctg ctggaacggg gcgcgaagga actcgcgacc gaaccggtga
     1441 agctgggcga gaaggcgatc aacgccttgc gcaagccttc gtcgctggcc gggcgggtcg
     1501 gggaaagtgc cgagggactg gccgaggtgg cccgggctct ggccgattcc gcgcccgacg
     1561 tgccgttgaa cgtgccgatc ggcccccatc gccggatcac c
//
LOCUS       DAOQFI010000560         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_52974_length_1599_cov_2.764896, whole genome shotgun sequence.
ACCESSION   DAOQFI010000560 DAOQFI010000000
VERSION     DAOQFI010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52974_length_1599_cov_2.764896"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /gene="tilS"
                     /locus_tag="PLE93_09245"
     CDS             <1..794
                     /gene="tilS"
                     /locus_tag="PLE93_09245"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNH87275.1"
                     /translation="QALSSSGPAVQLAEDEEALMRLAVDAVARSGAAGPGGSRGIPAK
                     SLEDQPPAVRRRMLRHLAEAELDRPIAVSPALASEVLRLAGMPEGGRLDLGGGDSLLV
                     EAGRVRVCPGGGTGHDAVPEPARIDLKAGVAAFGGWEIESSREVEAEVRSAFGDPWAA
                     FLDLDDLLIWLIEKSPGADDLLLQVRPWRFGDRIEPLGMSGSKSLQDVFTDALVPASR
                     RQSWPVLVVGEKVIWVPGLVRSRHLLIAGPDRDVISLHARPPFSI"
     gene            813..1376
                     /gene="hpt"
                     /locus_tag="PLE93_09250"
     CDS             813..1376
                     /gene="hpt"
                     /locus_tag="PLE93_09250"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNH87276.1"
                     /translation="MAATPPNELVSVGEVLITAEDLQRKVADLGAEVSRDYAGKDLVM
                     VGVLRGAVVFIADLLRHLTVPCEIDFMAVSSYGSSRDSSGVVRILKDLDDSITGRDVL
                     IVEDIVDSGLTLNYLMRNLQARDPASLEVCTLLTKPERRRVDVPIRYTGFEIPNQFVV
                     GYGLDFAQRYRNLDHVALLPEDAVPDR"
BASE COUNT          245 a          525 c          536 g          293 t
ORIGIN      
        1 tgcaggcgct cagctcgtcc ggcccggcgg tgcagctggc cgaggacgag gaagccctga
       61 tgcggctggc ggtcgacgcc gtcgctcgat ccggcgcggc cggtccggga gggagccggg
      121 gaattcccgc gaagtcgctt gaagaccagc cgccggccgt ccggcgccgc atgcttcgtc
      181 acctcgccga ggccgaactt gaccggccga tcgccgtttc gccggccctt gcctctgaag
      241 tcctgcgtct ggccggaatg cccgaaggag gcaggctgga cctcggcggc ggagactccc
      301 tgctggttga agcgggaagg gtacgggtct gcccgggcgg gggaacgggc cacgacgcgg
      361 tgcccgagcc ggcccggatc gacctcaagg ccggtgttgc cgccttcggt ggctgggaga
      421 tcgaatcctc aagggaagtc gaggccgagg tccgttccgc gttcggggat ccctgggcgg
      481 catttctcga tctcgatgac ctgctcatct ggttgatcga gaagtcaccc ggcgctgacg
      541 accttctgct tcaggtgcgg ccgtggcgtt tcggcgaccg catcgagccg cttggcatga
      601 gcggcagcaa gagcctccag gacgtcttca ccgacgcact cgtgcccgct tccagaaggc
      661 agagctggcc ggtcctggtg gtcggcgaga aggtcatctg ggtgccgggg ctggtccggt
      721 cacgccacct cctgatcgcg gggccggacc gggatgtcat tagcctccac gcccggccgc
      781 cgttctccat ctagacctct agactggcgg cgatggctgc gacgcctccg aatgaactcg
      841 tctcggtcgg cgaggtgctg atcaccgctg aagacctcca gcggaaagtc gccgacctcg
      901 gggccgaagt cagccgcgat tacgccggca aggacctggt catggtcggg gtgctgcgcg
      961 gcgcggtggt cttcatcgcc gacctgctcc ggcacctcac cgtgccctgc gagatcgact
     1021 tcatggcggt ctccagctac ggctcgtcgc gtgattcttc aggcgtggtg cggatcctca
     1081 aggacctgga tgactcgatc accggccgtg acgtgctgat cgtcgaggac atcgtcgatt
     1141 cgggcctcac gctcaactac ctgatgcgga acctccaggc ccgcgacccg gcgtcactgg
     1201 aggtgtgcac cctcctgacc aagccggaac gccgtcgggt cgacgttccg atccgctaca
     1261 ccggtttcga gatccccaat cagttcgtgg tcggttacgg gctcgatttc gcccagcgct
     1321 accggaatct cgatcatgtg gccttgctac cggaagacgc ggtccccgac cgttagcggc
     1381 ggcggcaaga aatcttcatc cgggaccgct ctcccatgcc ggttgccggg ttctccctgc
     1441 tagtctgaca attcggcctg aaggcaggta gagcctgtcg cgaggtcgtg gaaagagacc
     1501 caagatttga aacggttttt caagagcgcg gcattcccca tcctgctggt ggtcatcctg
     1561 gccttcgtcg cgcagcgcgt gatcagcccg gacagcggc
//
LOCUS       DAOQFI010000561         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_53466_length_1591_cov_2.841797, whole genome shotgun sequence.
ACCESSION   DAOQFI010000561 DAOQFI010000000
VERSION     DAOQFI010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53466_length_1591_cov_2.841797"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /gene="purD"
                     /locus_tag="PLE93_09255"
     CDS             <1..980
                     /gene="purD"
                     /locus_tag="PLE93_09255"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNH87277.1"
                     /translation="KAYAKEAMEEAGVPTAGYGVVSDRESALAKVAETGCPVVLKADG
                     LAAGKGVLICLTEEEAREAIEVFFTERRFGKTGVVIEEFLEGEELSLLAICDGERAIP
                     LAPAQDYKRIGEGDTGPNTGGMGSYSPVQTVSKDQLDELSALVHQPIVDLMARRGHPF
                     HGVLYAGLMMTADGPKVLEYNTRFGDPETQAVLPRLESDLVDLFLSASEPGGLAGTEV
                     SFADQFAVTVVLASAGYPASSSKGDVITGLDEAARSAEVVHAGTAEADGRIVTAGGRV
                     LNLTGTGPDAATARDRAYDAADAVNFDGKQMRRDIAERAVRRERNQKAT"
     gene            977..1450
                     /gene="purE"
                     /locus_tag="PLE93_09260"
     CDS             977..1450
                     /gene="purE"
                     /locus_tag="PLE93_09260"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNH87278.1"
                     /translation="MSEETQQPATLSSVEELFEEIDVEQPQVGIIMGSKNDKPKMQPA
                     GQALEDAGIAYEVRVMSAHRDPVLVAEYCTNAKMRGLKVIIAGAGMSAALPGVAAAHT
                     ELPVIGVPILGKSLGGLDALLSVAQMPPGVPVACVAVDGAKNAGVLATRIINLAG"
BASE COUNT          288 a          527 c          542 g          234 t
ORIGIN      
        1 ccaaggccta cgccaaggaa gcgatggaag aggccggcgt tccgaccgct ggttacgggg
       61 ttgtgagcga ccgggagagc gccctggcca aggtcgccga aaccggctgc ccggtggtgc
      121 tgaaggccga cgggctggcg gcaggcaagg gagttctgat ctgcctgacc gaagaggaag
      181 cccgcgaggc gatcgaggtc ttcttcaccg aacggcgctt cggaaagacc ggagtcgtga
      241 tcgaggaatt cctcgaaggc gaggagctct cgctgcttgc gatctgcgac ggcgagaggg
      301 cgatccccct ggccccggcc caggattaca agcggatcgg tgagggtgac accggcccga
      361 acaccggcgg catgggcagc tactcgccgg tccagaccgt cagcaaggat caactggacg
      421 aactgtcggc cctggtgcat cagccgatcg tcgacctgat ggcccgccgc ggccacccct
      481 tccacggggt gctctatgcg ggcctgatga tgaccgccga cggtccgaag gttctcgagt
      541 acaacacccg ctttggcgac ccggagaccc aggcggtact cccgcggctt gagtccgacc
      601 tggtcgacct cttcctttcc gcctcggagc cgggaggcct ggccggaacc gaggtcagct
      661 ttgccgatca gttcgcggtg accgtggtgc tggcctcggc cggttacccc gcctcgtcct
      721 cgaagggtga cgtgatcacc ggactcgacg aagcggcacg gtcggcggaa gtggttcacg
      781 ccggcacggc ggaagcggac ggacggatcg tcaccgccgg tggccgggtg ctgaacctca
      841 ccggtacggg acccgatgct gcaaccgcgc gcgatcgcgc ttacgatgcg gctgacgcag
      901 tcaatttcga tggaaaacag atgcgacgcg acatcgccga aagggctgtc cggcgcgaac
      961 gaaaccagaa agcaacatga gcgaagaaac ccagcagccg gcaacccttt cctcggtcga
     1021 agagctcttc gaggaaatcg acgttgaaca gccccaggtg gggatcatca tgggctcgaa
     1081 gaacgacaag cccaaaatgc agccggccgg acaggccctt gaggacgccg gcatcgccta
     1141 cgaggtgcgg gtcatgtcgg cccatcgcga ccccgtactg gtcgccgagt actgcaccaa
     1201 cgcgaagatg cgcggcctca aggtgatcat cgccggggcc ggcatgtcgg ccgccctgcc
     1261 cggagtcgcc gccgcccaca ccgagcttcc ggtgatcggc gtgccgatcc tcggcaagtc
     1321 cctgggcggg ctcgacgcgt tgctgtcggt ggcccagatg cctcccgggg tgccggtcgc
     1381 ctgcgtcgca gtcgacggcg ccaagaacgc cggcgtgctc gccacccgca tcatcaacct
     1441 ggccggctag caccgcatga tcaagcgcta cacccgcccc gggatggggg cggtctggac
     1501 ccagcaggcc aagttccagt cctggctgga cgtcgagctg gctgccaccg acgcctgggc
     1561 ggccgagggc gtggtcccgc ccgaagccgc t
//
LOCUS       DAOQFI010000562         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_53473_length_1591_cov_2.661458, whole genome shotgun sequence.
ACCESSION   DAOQFI010000562 DAOQFI010000000
VERSION     DAOQFI010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53473_length_1591_cov_2.661458"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLE93_09265"
     CDS             <1..648
                     /locus_tag="PLE93_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNH87279.1"
                     /translation="GIRHDPTAIEKVVFPNGEVDRPGRSKPVRVLSEAEAYEVTKILH
                     DNITSGTGTGAYTGCGGQAGKTGTTDNQVDAWFVGYQPNLTAAAWIGYPESNDLSTGS
                     DGGDVPASIWNSFFYNAAVPCEETPIPTETMDWGDFSGGYTVSPGTQSDYGPQPEDTT
                     GTDTGTGTGTDTGTGTVEPDGAYAPGVGQEPAGVGGGTGGTGGSTGGSTGGVTPG"
     gene            complement(680..>1591)
                     /locus_tag="PLE93_09270"
     CDS             complement(680..>1591)
                     /locus_tag="PLE93_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNH87280.1"
                     /translation="ISQWSELKEKGVEFDFIWLIPAVVMMMLYYIAGAGVWGLILRFL
                     GSPVPAMETQKIWAQPLLVRYIPGTVLFLLARILMAEKAGVQRRVTTAGIVYEQAVSI
                     AGALTIATWFLIDHPDLQDQPARWLPLLVLPVMVCLLHPRVFGPVSKRLLHALGRESL
                     PQLMPFRGVVVIYLAYVVVWAIMGVGVFFAARAVHHLNLDDLAIVAASQMIGFLAAVV
                     SAVTPAGLGVRDAAFAWAVKAALPSDSFGVGAVIAIAVRATQTVVELIYVGGVTALTR
                     KEGDEDEIELAEEAAEEAARLQTESGS"
BASE COUNT          306 a          542 c          537 g          206 t
ORIGIN      
        1 ggcatccgtc acgacccgac cgcgatcgag aaggtcgtct tcccgaacgg tgaagtggac
       61 cggccgggca ggtccaagcc ggtgcgggtc ctctccgagg ccgaggcgta cgaggtcacg
      121 aagattctgc acgacaacat caccagcggc accggcaccg gggcctacac cggctgcggc
      181 ggccaggccg gcaagaccgg caccaccgac aaccaggtcg acgcctggtt cgtcgggtac
      241 cagccgaacc tgaccgcggc cgcctggatc ggctacccgg agtcgaatga cctcagcacc
      301 ggctccgatg gcggtgacgt gcccgccagc atctggaaca gcttcttcta caacgcggcc
      361 gtgccctgcg aagaaacccc gatcccgacc gagaccatgg actggggcga tttcagcggc
      421 ggctacacgg tttcacccgg tacccagagc gactacggcc cccagcccga agacacgacg
      481 ggcaccgaca ccggaaccgg cacgggcacc gataccggta ccggcactgt cgagccggat
      541 ggcgcctacg cgcccggggt cggtcaggaa ccggccggcg tgggtggcgg caccggcggt
      601 accggtggca gcaccggcgg ttcaaccggc ggcgtcacgc ccggttagcc ggacggtcgc
      661 gggccggaac ccttcaggtt cagctgcccg attcggtctg caggcgggcc gcctcttcgg
      721 cggcttcttc ggccagttcg atctcgtctt cgtcaccctc cttgcgcgtg agcgcggtga
      781 ccccgccgac gtagatcaac tcgaccaccg tctgggtggc ccggaccgca atcgcgatga
      841 ccgctcccac cccgaagctg tccgaaggca gggcggcctt gaccgcccag gcgaacgccg
      901 cgtcacggac accgaggccg gcgggagtga cggccgagac cacggcggcg agaaatccga
      961 tcatctggga agcggcgacg atggcgagat cgtcgagatt gaggtgatgg accgcccggg
     1021 cggcgaagaa gacgccgacg cccatgatcg cccagaccac cacataggcg aggtagatga
     1081 cgacgacgcc acggaacggc atcagctgcg gcagtgattc gcgcccgagc gcgtggagca
     1141 agcgcttcga gacggggccg aagacgcggg gatgaagcag gcagaccatc accggcagga
     1201 ccagcaacgg gagccagcgg gccggctgat cctgcaggtc ggggtggtcg atcagaaacc
     1261 aggtggcaat ggtcagggca ccggcgatcg aaaccgcctg ctcgtagacg atgccggccg
     1321 tggtcacccg tcgctggacc ccggcctttt cggccatgag gatccgggcc agcaggaaca
     1381 ggacggtgcc ggggatgtaa cggaccagca gcggctgggc ccagatcttc tgggtctcca
     1441 tggccggaac cgggctgccg aggaaacgca ggatcaggcc ccataccccg gccccggcga
     1501 tgtaatagag catcatcatc accaccgccg ggatcagcca gatgaagtcg aactcgacgc
     1561 ccttctcctt cagctccgac cactgtgaga t
//
LOCUS       DAOQFI010000563         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_53508_length_1590_cov_4.029316, whole genome shotgun sequence.
ACCESSION   DAOQFI010000563 DAOQFI010000000
VERSION     DAOQFI010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53508_length_1590_cov_4.029316"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLE93_09275"
     CDS             <1..804
                     /locus_tag="PLE93_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNH87281.1"
                     /translation="PVSPGFPEVPLSYSALASYRECPARFFATRVLKLDEPERRHGDA
                     KQPLDPEMEIRKEIDATSFGVAVHELLELLPARRWIRPADAEIDSRLEANGVPGADAG
                     DLAREMLDGFLASELAGEIKGRRAEVETGLLVEVGGVMVRGFADLLVRDPVKPLILDY
                     KTNQLEETSIQELMSKYDLQRDLYALAVSKAAGVEELETAFVFLRAPDQPVRDHYDSK
                     RLSEAENSIAGLIDDIVAGRYLGGPDAGRQPCGDCWACETLGVQKGTSG"
     gene            complement(783..1115)
                     /gene="xseB"
                     /locus_tag="PLE93_09280"
     CDS             complement(783..1115)
                     /gene="xseB"
                     /locus_tag="PLE93_09280"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNH87282.1"
                     /translation="MSEETPEAPEHSYESATARLDEIIQRLDSGDAQLRETLELCEEA
                     KGLIEYCAGELAAVDQGLKELKLEDLASSLTGPGNGGSEPPVPEPAGPPEPEHEAPPP
                     PDQPDVPF"
     gene            complement(1108..>1590)
                     /locus_tag="PLE93_09285"
     CDS             complement(1108..>1590)
                     /locus_tag="PLE93_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87283.1"
                     /translation="VPARRRRRLREIRAAADRGIQGRRGSAQRVLDQCLLPAASRTNR
                     LIEAAGTRNGARPGALQARVLGRLGFAGRKLETERVALRAHDPQRTLERGYARVEDRK
                     GDPVVDSSGARRAGQVRIRFADDAVDAVVGDTRRRRSRQNEVEQEFEQIRMEGLDEGE
                     "
BASE COUNT          245 a          525 c          541 g          279 t
ORIGIN      
        1 ccggtatctc ccggttttcc ggaagtcccg ctctcctact ccgcccttgc ctcctaccgg
       61 gagtgtcccg cccggttctt cgccaccagg gtgctgaagc tggacgaacc ggaacgccgg
      121 cacggcgacg cgaagcaacc gctggatccg gaaatggaga tccgcaagga gatcgatgcc
      181 acctcattcg gggtggcggt gcacgagctg ctggaactgc tgcccgcacg ccgctggatc
      241 aggccggccg atgccgaaat cgactcgaga ctggaggcga acggggttcc gggagcagat
      301 gccggcgacc tggcccggga gatgctcgac gggtttcttg cttccgaact cgccggggaa
      361 atcaagggca gaagggccga ggttgaaacc ggtctgctgg tcgaggtcgg gggcgtgatg
      421 gtccgcggct ttgccgatct cctcgtccgc gacccggtca aacccctgat cctcgactac
      481 aagacaaacc agctcgagga gacctcgatc caggagttga tgtcgaagta cgacctccag
      541 cgggaccttt acgcgttggc agtgtcgaag gccgccggtg tcgaagaact ggaaaccgcg
      601 ttcgtctttc tgcgagcgcc ggatcagccg gtgcgcgacc actacgactc aaagcgtctc
      661 tcggaagccg agaattcgat cgccggtctc atcgacgaca tcgtggccgg ccggtacctg
      721 ggcggaccgg acgcgggaag acaaccctgc ggcgattgct gggcctgcga aaccctcgga
      781 gttcagaagg ggacgtcggg ctgatccggg ggtggcggag cctcgtgctc cggctcgggc
      841 ggcccggccg gttcggggac cggtggctcc gagccgccat ttccggggcc ggtcagggag
      901 ctggcgaggt cctctagctt gagttccttg aggccctggt cgacggcggc gagttctccg
      961 gcgcagtact cgatcaggcc tttggcttcc tcacagagtt cgagggtttc gcggagctgg
     1021 gcgtcgcccg agtcgaggcg ctggatgatc tcgtcgagcc gcgcggtggc cgactcgtag
     1081 ctgtgctcgg gcgcttcggg cgtttcttca ctcaccttcg tccagtcctt ccatcctgat
     1141 ctgctcgaac tcctgctcga cttcattctg gcgcgaccgg cggcgtctcg tgtcgccgac
     1201 cacggcatcg accgcgtcgt cggcgaaacg aatcctgacc tggccggccc gacgggctcc
     1261 gctggaatcg actaccggat ctcccttgcg gtcctcgacc cgggcataac cccgctcgag
     1321 ggtcctttgt ggatcgtggg cccggagcgc gacccgctcg gtctcgagct tcctcccggc
     1381 gaatccgagc cggcccagga ccctggcctg gagcgcgccg ggccgggccc cgttccgggt
     1441 accagcggcc tcgatcaggc ggttggtccg ggaagccgcc gggagcaggc actgatcaag
     1501 cacccgctgg gccgagcctc gacggccctg aattccgcgg tcggcggcgg cccggatttc
     1561 ccgaagacga cgtcgacgcc gagctgggac
//
LOCUS       DAOQFI010000564         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_54000_length_1583_cov_4.244764, whole genome shotgun sequence.
ACCESSION   DAOQFI010000564 DAOQFI010000000
VERSION     DAOQFI010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54000_length_1583_cov_4.244764"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLE93_09290"
     CDS             complement(<1..203)
                     /locus_tag="PLE93_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNH87284.1"
                     /translation="MSRQSEITPEGLKKLEEEVEYLSTVRRREVADRIKEARDFGDIS
                     ENAEYDDAKNEQAQLEAQIVQLE"
     gene            complement(309..1208)
                     /locus_tag="PLE93_09295"
     CDS             complement(309..1208)
                     /locus_tag="PLE93_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH87285.1"
                     /translation="MTSGAGLSDRVEAWRLKGADEEFRGRQIHACFQDGEGPLVVLLH
                     GFPTCSYDWRYLFELMPDRALLAFDFLGFGLSDKPRDHSYTLSWQADLTEELICRHWA
                     GKPAFICAHDFGASVATELLARDLADELRFELAGMLLFNGSILVERSNPTLAQKLLRG
                     PLGPLAARFSNEAFFRRQFASVFGGAHPLTDADATDYWSLMTFNDGNRLGHKLVSYMD
                     ERAIYADRWHGAVSRWKGDLHLAWGMQDPVAVPDILDGLLDMRPDLPVVRMPELGHYP
                     QVEDPDRFARILTQSLATSNSSL"
     gene            complement(1205..>1583)
                     /locus_tag="PLE93_09300"
     CDS             complement(1205..>1583)
                     /locus_tag="PLE93_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNH87286.1"
                     /translation="VPMIISGGLSSAEAARRAYEESNADAVMIARGSLGYPWVFAELT
                     GQEVEPPTRESVMDELAWVMDRAEEHLGEVRAGRYLRKFYPWYLDQLDVPKGVRNELC
                     QTAGLDRAREIVAGIRNGEPVIL"
BASE COUNT          278 a          497 c          544 g          264 t
ORIGIN      
        1 tcctcgagct ggacgatctg cgcttcgagc tgggcctgct cgttcttggc gtcgtcgtac
       61 tcggcgttct cggaaatgtc accgaagtca cgggcttcct tgatccggtc ggccacctcg
      121 cgacggcgta ccgtcgacag atactcgacc tcttcttcga gcttcttcag accttcgggc
      181 gtgatttcag actggcgact cacaggtatc aacctctctt gggaaggccg gcaatcgaag
      241 cccggccctg aaaatgacga acgccctgag cggcaccagg gggtaccggc agggcagcgg
      301 acgggcgatt ataacgagga gttgctcgtg gccagggact gggtcaggat ccgggcaaag
      361 cggtccggat cctcaacctg cgggtaatgc cccagttccg gcatgcggac caccgggaga
      421 tccggcctca tgtcaagcag gccgtcgagg atgtcgggta ctgcaaccgg atcctgcatg
      481 ccccaggcga ggtgaagatc gcctttccat cgggaaaccg cgccgtgcca gcggtcggcg
      541 tagatcgccc gttcgtccat gtacgagacc agtttgtgac cgagccggtt gccgtcgttg
      601 aaggtcatca ggctccagta atcggtggcg tcggcatcgg tcagcgggtg ggcgccgccg
      661 aagaccgagg cgaactgccg ccggaagaag gcctcgttcg agaagcgggc ggccagcggg
      721 ccaagcgggc cgcgcagcag cttctgggcc agggtcgggt tcgatcgttc aaccaggatc
      781 gagccgttga agagcagcat cccggccagt tcgaagcgca gttcatcggc caggtcccgg
      841 gccagcagct cggtcgcgac cgaggccccg aagtcatggg cgcagatgaa ggcaggtttg
      901 ccggcccagt ggcggcagat cagttcctcg gtcaggtcgg cctgccagga gagcgtgtag
      961 gagtggtcgc gcggcttgtc ggaaaggccg aaaccgagaa agtcgaaggc gagcagggcc
     1021 cggtccggca tcagctcgaa caggtagcgc cagtcatagg aacaggtcgg gaagccgtgg
     1081 agcaggacga ccagcgggcc ttcgccgtcc tggaagcagg cgtggatctg gcggccgcga
     1141 aactcctcgt cggcgccttt gagccgccag gcctcgaccc ggtcggagag cccggctccg
     1201 ctggtcacag gatcaccggc tctccgttgc ggattccggc cacgatctcg cgggcccggt
     1261 cgagaccggc ggtctggcag agctcgttgc ggaccccctt cggcacgtcg agctgatcca
     1321 ggtaccaggg gtagaacttg cgcaggtagc gaccggccct tacctcgccg agatgttcct
     1381 cggcccggtc catgacccag gccagctcgt ccatgaccga ttcacgggtc gggggctcga
     1441 cctcctggcc agtgagttcg gcaaagaccc aggggtagcc gagcgagccg cgggcgatca
     1501 tcaccgcgtc cgcattggac tcttcgtagg cccggcgggc cgcctcggcg ctgctcaggc
     1561 cgccggaaat gatcatgggc act
//
LOCUS       DAOQFI010000565         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_54242_length_1580_cov_2.197377, whole genome shotgun sequence.
ACCESSION   DAOQFI010000565 DAOQFI010000000
VERSION     DAOQFI010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54242_length_1580_cov_2.197377"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /gene="tilS"
                     /locus_tag="PLE93_09305"
     CDS             complement(<1..610)
                     /gene="tilS"
                     /locus_tag="PLE93_09305"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNH87287.1"
                     /translation="MLVSGGADSVALLLGLHLVLGAGHLVALHVNYGLRPTADDDERL
                     VRRICEQLEVELVVLRAGEAEGNMQAWARKIRLDEAERIRSERGLDWIAVGHNRSDQV
                     ETFIYRLVSSPGARSLLAMPPRSGNLIRPLLTLDRGLIRTMLERVTHSEDPTNDDPAF
                     ARNRIRLEVMPALEQVNSGAELNVVRTRAELAEDEEVLMRLAV"
     gene            complement(711..>1580)
                     /locus_tag="PLE93_09310"
     CDS             complement(711..>1580)
                     /locus_tag="PLE93_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH87288.1"
                     /translation="VMTVMGTPSDREQSLSARDIEAFKRMGEVAEAGLPVEALLQLTR
                     VYAQAVRRIADAEVRLFHLFVHEPMIRDGLSEIEMAEELGDLVRRTSPTAVPLFQYLH
                     DRYLRYFLEQDVIGHMESDFGGDTNLDQVRTTLCFIDLTGFTRFTEQEGDETAFSVVE
                     QFTEAVEATLPPEAGIVKTIGDEVMVVSPDPASLAEWAVGFLALFEDRPKPRAGIHHG
                     AVVFRDGDYYGSQVNLAHRVVNRALGGEVLITDAVAAAIEDHPDLETDAIGEVDLKGF
                     PVPTPLFLVRARS"
BASE COUNT          267 a          537 c          519 g          257 t
ORIGIN      
        1 cgaccgcaag ccgcatcagg acctcctcgt cctcggccag ctcggctctg gtccgcacca
       61 cgttgagctc cgccccggag ttgacctgct cgagcgccgg catgacctcc agccggatcc
      121 ggttgcgggc gaaggccgga tcgtcgttgg tcgggtcttc cgaatgggtg acgcgttcaa
      181 gcatcgtccg gatcaggccg cgatcgagcg tgaggagcgg cctgatcagg tttcccgacc
      241 ttggcggcat cgccagcagg gacctcgcgc ccggcgagga gacgaggcgg tagatgaagg
      301 tctcgacctg atcggagcgg ttgtggccga ccgcaatcca gtccagaccc cgctcgctgc
      361 ggatccgctc cgcttcatcc agccgaatct tccgggccca ggcctgcatg tttccctctg
      421 cctcacccgc ccggaggaca accagttcga cctccagctg ctcacagatc cggcggacca
      481 gccgctcgtc atcgtcggcg gtgggccgga ggccgtagtt gacgtggagt gcgaccaggt
      541 gcccggcgcc gagaaccaga tggaggccga gcagcagcgc gaccgaatcg gccccgcccg
      601 aaacgagcac gacaccgcga tccccttccg ataccagtcc cgattccctg atcgcgccgc
      661 caatgaaatg tgcgggcttg cccccgaacc ggaacgattc gaacccgggg tcaggagcgg
      721 gcacgaacca gaaacagcgg ggtcgggacc gggaagccct tgagatcgac ctcgccgatc
      781 gcatcggtct cgaggtccgg gtgatcctcg atcgccgccg ccacggcatc ggtgatcagc
      841 acctcgccgc caagggcccg gttgacgacc cggtgggcga ggttgacctg gctgccgtag
      901 tagtcgccat cccggaatac gaccgcaccg tgatggatgc cggcccgcgg cttcggccgg
      961 tcctcgaaca gggccaggaa cccgaccgcc cattcggcca gtgaagcggg gtcgggcgag
     1021 accaccatta cctcgtcgcc gatcgtcttg acgatcccgg cctcgggcgg cagggtcgcc
     1081 tcgaccgctt cggtgaactg ctcgaccacc gagaacgccg tttcgtcacc ctcctgttcg
     1141 gtgaaccggg tgaacccggt caggtcgatg aagcagagcg tggtcctgac ctggtcgagg
     1201 ttggtgtcgc caccgaagtc ggactccatg tgcccgatca cgtcctgttc gaggaagtac
     1261 ctgagatacc ggtcatgcag gtactggaag agcggcaccg cggtgggcga ggtgcggcgg
     1321 acaaggtcgc cgagttcctc ggccatttcg atctcgctca ggccgtcacg gatcatcggc
     1381 tcatggacga agagatggaa gagccggacc tcggcgtcgg cgatccgtcg tacggcctgg
     1441 gcgtacaccc tggtcagctg gagcagggct tcgaccggca ggccggcctc ggccacctcc
     1501 cccatccgct tgaacgcttc gatgtctctg gcggagaggc tctgctcgcg gtcggacgga
     1561 gtgcccatca cggtcatgac
//
LOCUS       DAOQFI010000566         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_54264_length_1579_cov_4.169291, whole genome shotgun sequence.
ACCESSION   DAOQFI010000566 DAOQFI010000000
VERSION     DAOQFI010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54264_length_1579_cov_4.169291"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLE93_09315"
     CDS             <1..681
                     /locus_tag="PLE93_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNH87289.1"
                     /translation="EIVKRSWSGETFDHEGEFFHLEKGGLRLGFGEVDPPRVYFGGAS
                     PPALEVAARHADTWLTWGEPVDTVAGRVAPVREKAEAAGREISCGLRIHVISRDTEEE
                     AWLEADRILEAIDPEQVAAAREKFARMESTAQARMTDLSHSSEGDALDRDALVIGPNL
                     WAGVGLVREGAGTAIVGSHQQVAERLLEYRDIGIDEFILSGYPHLEEALNFGENVRPL
                     LDAATVFA"
     gene            701..>1579
                     /locus_tag="PLE93_09320"
     CDS             701..>1579
                     /locus_tag="PLE93_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87290.1"
                     /translation="MRLINNPVRRFDFVQKLEWFLLSAVTMILIIRTQLWLTHYPQLG
                     GGGLHIAHLLYGGLFMVIAIWLGQIYLNRWGRTVQAILGGIGFGFFIDELGKFITEDN
                     NYFFKPAAGIIYLIFIFMFLAIRELSRRQSLDPRSALANALSLLPGTAIGEYRKDEAE
                     RAGRLLDMADQSDPMVGKAREMFRQAEVVPGRPPSRLTGTVNRIHGWIRGLTQRRHFE
                     GIVIAVVIIWALSSLSSVVAIDLDLGGVQDHQGPEVNSGVLGSLESLSALVSVVLVFI
                     GAWAMVRRDHARAYRYF"
BASE COUNT          247 a          533 c          516 g          283 t
ORIGIN      
        1 gagatcgtca agcgctcctg gtcgggcgaa accttcgacc acgaaggcga gttcttccac
       61 ctcgagaagg gcggcctgcg cctcgggttc ggtgaggtcg atccgccccg ggtctatttc
      121 ggcggggcct ctccgccggc cctcgaggtc gccgccaggc acgccgacac ctggcttacc
      181 tggggcgagc cggtcgacac ggtggccggg cgggtcgcgc cggtcaggga gaaggcggag
      241 gctgccggtc gcgagatcag ttgtggactg cgcattcacg tgatcagccg tgacaccgag
      301 gaagaggcct ggctcgaggc cgaccgcatc ctcgaggcga tcgacccgga gcaggttgcc
      361 gccgcgcgcg agaagttcgc ccgcatggaa tcgaccgccc aggcccggat gaccgacctc
      421 agccactctt ccgaaggaga cgcgctcgac cgggatgccc tggtcatcgg acccaacctc
      481 tgggccgggg tcgggctggt ccgcgaaggg gccggcaccg cgatcgtcgg cagccatcag
      541 caggttgccg agcgcctgct cgaataccgc gacatcggca tcgacgagtt catcctctcc
      601 ggctatccgc atctcgagga agcgctcaac ttcggcgaga acgtgcgccc gctgctcgac
      661 gccgcgaccg tcttcgcctg agcgaagcta cagtcagccc gtgcggctga tcaacaatcc
      721 ggtcaggcgg ttcgacttcg tccagaagct cgaatggttc ctgctgtcag cggtgacgat
      781 gatcctgatc atccgcacgc agctctggct gacccactac ccgcagctgg gcggcggtgg
      841 tcttcacatc gcccatctgc tctacggcgg cctcttcatg gtgatcgcga tctggctcgg
      901 ccagatttac ctgaaccgct ggggccggac cgtccaggcg atcctcggcg ggatcggttt
      961 cggcttcttc atcgatgaac tgggcaagtt catcaccgag gacaacaact acttcttcaa
     1021 gccggccgcc ggaatcatct acctgatctt catcttcatg ttcctggcca tccgggagct
     1081 ctcccgccgg cagagcctgg acccccggtc ggcgctggcc aacgcgcttt cgctgctgcc
     1141 gggcaccgcg atcggggaat accggaagga cgaggccgag cgggccggca ggctgctgga
     1201 catggccgac caatccgatc cgatggtcgg caaggcaagg gagatgttcc gccaggcgga
     1261 agtggttccg ggccggccgc cttcgcgact gaccgggacc gtgaaccgca tccacggctg
     1321 gatccgcggc ctcacgcagc gccgtcattt cgaggggatc gtgatcgcgg tggtgatcat
     1381 ctgggcgctc tcctcgcttt cctcggtggt cgcgatcgat ctcgacctgg gcggggtcca
     1441 ggaccatcag ggaccggaag tcaactccgg ggtcctcggt tcgctcgagt ctctctcggc
     1501 cctggtctcg gtcgtcctcg tcttcatcgg cgcctgggcg atggtccggc gggaccacgc
     1561 ccgcgcctac cgttatttc
//
LOCUS       DAOQFI010000567         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_54289_length_1579_cov_2.665354, whole genome shotgun sequence.
ACCESSION   DAOQFI010000567 DAOQFI010000000
VERSION     DAOQFI010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54289_length_1579_cov_2.665354"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..874)
                     /locus_tag="PLE93_09325"
     CDS             complement(128..874)
                     /locus_tag="PLE93_09325"
                     /inference="COORDINATES: protein motif:HMM:NF018496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HNH87291.1"
                     /translation="MSGPSGTGSHLDIGGVFEKTGDIYSKAFGTFWIVALVLLIPPAI
                     LQWLIGDSALGGLVSAIVNIFATAWLAGGVVRIVQDVEADGQVDYSVGDLLGSVWPRL
                     LAIVGLQIVVGIIIFFGLIFFIIPGIILALVLVVSLPALVVEDGGIFDSMGRSAALTK
                     DNRMRILAVGILMILILVGIAIVAGLLSAITPIIGALAMLVLGVLLYPYIYMLTTVLY
                     FRLVELKEGGVVAVEETVIVEEDAGPPPAV"
     gene            1039..>1579
                     /locus_tag="PLE93_09330"
     CDS             1039..>1579
                     /locus_tag="PLE93_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNH87292.1"
                     /translation="MTSASGTSTLTQDSPRFTGKELRRKASRESHADWEPAPGRDPLK
                     ILLAQGESRVQDLLPIRYGRMSASPFAFYRGGAAIMAADLAHTPATGVRVQACGDAHI
                     SNFGGYAAPDRKLVFDLNDFDETLPAPWEWDVKRMAASAAGTTAPSPAAAQRRPARPS
                     RLSSSHGTDTNAVGAIACQL"
BASE COUNT          301 a          550 c          496 g          232 t
ORIGIN      
        1 gggcctccgg ggctctctct caaaagaaca agggccgcgg ctggtgctct gcgcaccggc
       61 cgcggccctt ttgttgactg cgctgatccg ggggctccca cccggaccgg cgcaagtttg
      121 ccgccgccta cacggcgggc ggcggtccgg cgtcctcctc gacgatcacg gtctcttcga
      181 ccgcgaccac gccgccctcc ttcagctcaa ccagcctgaa gtaaagaacc gtcgtcagca
      241 tgtagatgta cgggtagagc agcaccccga gcacgagcat tgcgagcgcc ccgatgatcg
      301 gcgtgatcgc cgaaaggagg ccggccacga tcgcgatgcc gaccaggatc aggatcatca
      361 ggatgccgac cgcgaggatc cgcatgcggt tgtccttggt cagtgcggcg ctgcggccca
      421 tcgaatcgaa gattccgccg tcttcgacca caagggccgg cagcgagacg accagcacca
      481 gggcgagaat gatgcccggg atgatgaaga agatcagccc gaagaagatg atgatcccga
      541 ccacgatctg aaggccgacg atcgccagca ggcgcggcca gaccgaaccc aggagatcac
      601 cgaccgagta gtcgacctgt ccgtccgctt cgacgtcctg gacgatgcgc accaccccgc
      661 cggccagcca ggcggtggcg aagatgttga cgatcgccga aacgaggccg ccgagggcgc
      721 tgtcaccgat cagccactgc aggattgccg gcgggatcag gaggacaagg gcgacgatcc
      781 agaacgtgcc aaaggccttc gagtagatgt ctccggtctt ttcgaacacc ccgccaatgt
      841 cgaggtgact gccggtaccg cttggaccgc tcatttgttg ctcctccttg ggatttcagt
      901 tcgggaagac ccgtgaaacc gcctttacac gccatcggcc agatacctcc ccgcgggaaa
      961 agtggttcat tcgggccggg tggcgattca tccggttgga atgagaaaca gacgaccggc
     1021 gccaatatcc tcaacgccat gacgagcgca tcaggcacct ccaccctcac ccaggactcc
     1081 cctcgattca ccgggaagga actgcgcagg aaggcttcga gggaaagcca cgcggactgg
     1141 gagccggcgc cgggccgtga ccccctgaag atcctgctgg cccagggcga gagccgggtc
     1201 caggaccttc tgccgatcag gtacggccgc atgtcggcct cccccttcgc cttttaccgg
     1261 ggcggcgccg cgatcatggc cgccgacctg gcccacaccc cggccaccgg ggttcgggtc
     1321 caggcctgcg gtgacgccca catctcgaac ttcgggggct acgccgcgcc ggaccgcaag
     1381 ctcgtcttcg acctgaacga cttcgacgag accctccccg ccccctggga atgggacgtc
     1441 aagcggatgg ccgccagcgc cgcgggcacc accgccccga gtcccgcggc cgcccagagg
     1501 aggccggcgc ggccatcgcg gctcagctcg tcccacggaa ccgacacgaa cgcggtcggt
     1561 gcgatcgcct gccagctca
//
LOCUS       DAOQFI010000568         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_54423_length_1577_cov_2.299606, whole genome shotgun sequence.
ACCESSION   DAOQFI010000568 DAOQFI010000000
VERSION     DAOQFI010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54423_length_1577_cov_2.299606"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLE93_09335"
     CDS             complement(<1..618)
                     /locus_tag="PLE93_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNH87293.1"
                     /translation="MRRLVMLAVTAFVVGYLSTGSVDQVFAAIGKIPQALGVTELVDR
                     IGADTTAAKTRSKPRTATVSRVVDGDTLEVTFANGKDADVRLLAYDTPEPYRYGTPEC
                     GSKRASAFTTKLAYGKPVTLTVDSTQDTVDMYGRLLRYARIDGKDLGRQVIAAGWGAP
                     YVFNEDSPPKNIGYYRTAATNAKNAGRGVWGLCDGNFHSEEDQPWS"
     gene            complement(639..1412)
                     /locus_tag="PLE93_09340"
     CDS             complement(639..1412)
                     /locus_tag="PLE93_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87294.1"
                     /translation="MANTAKAKPNNSMMALELARKATKSRVEVLDALKANDVSLEKAL
                     DNPAIGNVRIEMVLRALPCSKTRTTDRSNPISRRNAAAILARAGISPCKPVGSLRNIV
                     TRGNLLNVAHSVSGRHAWIYESDSPPVLVERHTKGTKGRTEIQIRAAEKSLATRRRRR
                     EIKDQLRSGSMSLEEAITDPAAEKWPIGEIVVNLRRLNPDGTVKTRAWSTQILRTLNR
                     VGMPETAKAGQLTAYDRRMILLAFADEGFLAVKKGLVAA"
BASE COUNT          229 a          472 c          513 g          363 t
ORIGIN      
        1 ggaccagggc tggtcttctt cggaatggaa gttgccgtcg caaaggcccc agacgccgcg
       61 gccggcgttc ttggcgttgg tggctgcggt ccggtagtag ccgatgtttt tcggggggct
      121 gtcctcgttg aacacgtaag gggcgcccca tccggcggcg atcacctggc ggcccaggtc
      181 cttgccgtcg atccgggcgt agcggagcag ccggccgtac atgtcgaccg tgtcctgggt
      241 tgaatcgacg gtcagcgtta ccggctttcc gtaggccagt ttcgtggtga acgccgaggc
      301 tctcttggac ccacattccg gggtgccata gcggtagggc tcgggggtgt cataagccag
      361 aagccgcaca tcggcgtctt ttccgttcgc gaatgtcacc tcgagcgtgt caccgtcaac
      421 aacccggctg accgttgctg tccgcggctt cgacctggtt tttgcggcag tggtgtcggc
      481 gccgatcctg tcgaccagtt ccgttacccc gagcgcctgc gggatcttgc cgatcgcggc
      541 aaacacctga tcaacgcttc cggttgacag atacccgacc acaaaggcgg tcacggccag
      601 catcaccagc cggcgcatga ggtgtcgtgt tgcccatctc atgctgccac caggcctttc
      661 ttgaccgcga ggaatccttc gtctgcgaac gccagcagga tcatcctgcg gtcgtaggcg
      721 gtgagctgcc cggccttcgc ggtttccggc attccgaccc ggttgagggt gcggaggatc
      781 tgggtgctcc aggcacgtgt cttcacggtg ccgtcagggt tgagccgtcg aaggttcacg
      841 acgatctccc cgatcggcca cttttccgct gccggatcgg tgatcgcttc ctccagcgac
      901 attgatcctg agcggagctg atccttgatt tcgcggcgtc gcctgcgagt cgcgagggat
      961 ttctcggcag cccggatctg tatctcggtt ctgcccttcg tccccttggt gtgacgttcg
     1021 accagcaccg gcgggctgtc gctctcgtag atccatgcgt gtctgccgga caccgagtgg
     1081 gcgacgttca gcaggtttcc gcgggtgact atgttgcgaa gcgatccgac cggtttgcac
     1141 ggggagatcc cggcccgggc aaggatggcc gcggcgtttc gtctcgagat cgggttgctg
     1201 cggtcggtcg tcctggtttt gctgcaggga agcgctctga ggaccatttc gatccggacg
     1261 tttccgatcg ccggattgtc aagcgctttc tcaagcgaca catcgttggc tttcagggcg
     1321 tcgagtactt cgacccttga cttggtggct ttgcgggcca gttcgagggc catcatgctg
     1381 ttgttcggtt ttgctttggc tgtgttggcc atggggtttc ctttcttcag gcttcaagct
     1441 cggggtagcg gccagcctcg aagcgttcga ggaagccggt aacaaccgag cctttgatca
     1501 gccggaagct ccgttcgccg tcgagcgagt cgacgacgat ccggtgctcg gctatttcga
     1561 ggtgcgcggc gatcaaa
//
LOCUS       DAOQFI010000569         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_54547_length_1575_cov_3.118421, whole genome shotgun sequence.
ACCESSION   DAOQFI010000569 DAOQFI010000000
VERSION     DAOQFI010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54547_length_1575_cov_3.118421"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLE93_09345"
     CDS             complement(<1..496)
                     /locus_tag="PLE93_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNH87295.1"
                     /translation="MSLKLGLYLGYWGIGPRGKDAIEAVQFAESIGYESVWVAESYGS
                     DCVSVLAWLAAQTEKINLGAAIMQVPARPPAAAAMAGATIDYLSNGRFMFGFGPSGPQ
                     VSEGWYGVPYSKPWGRTKEYVEVVREIVAREEKLDHQGEHYQLPLPEGQGKPFRHVPL
                     FPELR"
     gene            566..>1575
                     /locus_tag="PLE93_09350"
     CDS             566..>1575
                     /locus_tag="PLE93_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNH87296.1"
                     /translation="MTEAATQVLPADDPGRGLSGSEAAERLRSLGPAQPHSSRSTSSI
                     VAGNVFTLFNLIIGIFFVLILSLGLIADAIFGMIAIVNTWIGIRQEMKAKETLDRLAI
                     LVAPRAEVIRDGQPLELLADEVVPGDLVRISPGDQLVADGRMTSSRGLTVDESMLTGE
                     SDAIGKSPGDEVFSGSFVISGSGFCEVTAVREESYAGKIAGEAKAFRHPPSPLQEEVN
                     QVILVCTWVLLPLAALLIASLIVRQVGLVDAAQTATAGLITLIPEGLVLLMSVTFAVA
                     AVKMARRDTLVQQMSATESLASVDTICVDKTGTLTDGDLTVVEVIPAKVTDRKLAARE
                     LGR"
BASE COUNT          258 a          537 c          511 g          269 t
ORIGIN      
        1 gccggagttc cgggaacagc gggacgtgcc ggaagggctt gccctggcct tcgggcaggg
       61 gcagctggta gtgctcgccc tgatggtcga gcttctcctc acgggcgacg atctcgcgga
      121 ccacctcgac gtattccttg gtccggcccc agggcttgga gtagggaacg ccgtaccagc
      181 cctcggaaac ctgcgggccc gaggggccga agccgaacat gaagcggccg ttcgagaggt
      241 agtcgatcgt ggcgccggcc atcgcggcgg cagccggcgg gcgggccggg acctgcatga
      301 tcgccgcgcc gagattgatc ttctcggtct gggcggcaag ccaggcgagg acgctgacgc
      361 agtccgatcc atacgactcg gccacccaga ccgattcgta gccgatgctt tcggcgaact
      421 gcaccgcctc gatcgcatct tttccgcggg gcccgatgcc ccaataaccg agatacagcc
      481 caagcttcag tgacatgcgg gcatcctacc cgaaagtaca aattctgttt ttgttccaac
      541 cgggcaaacc ggtaacaatc attccgtgac tgaagcggcc acccaggtac tcccagccga
      601 tgatccgggc cgcggcctgt ccggttccga agcggccgag cgcctgcgca gcctcgggcc
      661 cgcccagccc cactcctcgc ggtcgacctc cagcatcgtc gccggcaacg tcttcacgct
      721 cttcaacctg atcatcggga tcttcttcgt cctgatcctc tcgctcgggc tgatcgccga
      781 tgcgatcttc ggaatgatcg cgatcgtcaa cacctggatc ggcattcgcc aggagatgaa
      841 ggcgaaggag accctggacc ggctggcgat cctggtcgcc ccgcgggccg aggtgatccg
      901 cgatggccag ccgctggagc tgctggccga cgaggtggtg cccggcgacc tggtccggat
      961 ttctcccggg gaccagctgg tcgccgacgg ccggatgacc agctcccgcg gcctgaccgt
     1021 cgacgaatcg atgctgaccg gcgagtccga tgcgatcggc aagtccccgg gcgacgaggt
     1081 cttctccggc agcttcgtga tctccggttc cggcttttgc gaagtgaccg cggttcggga
     1141 ggagagctat gccgggaaga tcgccggcga ggcgaaggcc ttccgccacc cgccttcacc
     1201 gctccaggaa gaggtcaacc aggtgatcct ggtctgcaca tgggtcctgt tgccgctggc
     1261 cgcgctcctg atcgcgtcgc tgatagtccg ccaggtcggg ctggtcgacg ccgcccagac
     1321 cgcgaccgcc ggcctgatca ccctgatccc cgagggcctg gtcctgctga tgagcgtgac
     1381 cttcgccgtc gcggccgtga agatggcccg gcgggacact ctggtccagc agatgagcgc
     1441 gaccgaatca cttgcttcgg ttgacacgat ctgcgtcgac aagacgggca ccctgaccga
     1501 cggcgacctg accgtggtcg aggtgattcc ggccaaggtc accgaccgca agctcgccgc
     1561 ccgcgagctc ggccg
//
LOCUS       DAOQFI010000570         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_54597_length_1574_cov_3.123766, whole genome shotgun sequence.
ACCESSION   DAOQFI010000570 DAOQFI010000000
VERSION     DAOQFI010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54597_length_1574_cov_3.123766"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLE93_09355"
     CDS             <1..291
                     /locus_tag="PLE93_09355"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HNH87297.1"
                     /translation="WFLLGIGMLLGAIFPVALEWLAKAMGVRDVTIALFSVTLLILLA
                     LSLNFSVIASRQSKQITRLAQQQAIDGVEEEAASPAASSRGETPAPREQAPR"
     gene            complement(230..1330)
                     /locus_tag="PLE93_09360"
     CDS             complement(230..1330)
                     /locus_tag="PLE93_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87298.1"
                     /translation="MITLEESTSHQPRVRAELEAALRQGPSHAYLFRGPSGSGKARAA
                     RAFAAGILSAGSADPEDSRRRALLDPSPHPDLVWLRPKGMSHAVQEIRDRIIHQAPLS
                     PFEGSARVFVIEAADALNEESQNAMLKTLEEPAPHAHLILLSAESEGLLPTIASRCQL
                     IEFGSLPFEVIEAELDGLASPEALHSAAKLAGGDLDKARFLAGKRGSDLRVAAERLMT
                     CALEDEFAGSPWLDLLAKARSAGEHSGELVQADFDEEKEEGIKHTKTEIEEAVRRAQR
                     KTRTGMLDLGLHLAAAWARDWATVIAGAPDLAFNQDRLEVLEAQAEGIELGQARDAVS
                     QIQETRRSFQLNVSEELALEALAFRLEKTLRD"
     gene            complement(1380..>1574)
                     /locus_tag="PLE93_09365"
     CDS             complement(1380..>1574)
                     /locus_tag="PLE93_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87299.1"
                     /translation="FGLDRAGSDDRFEAEGVRFQERVAEAYEDIARREPNRVVPIDAT
                     GSPEQVHRLILAEVEARLPA"
BASE COUNT          218 a          541 c          538 g          277 t
ORIGIN      
        1 tggtttctgc tcggaatcgg catgctgctc ggcgcgatct tccccgtcgc cctcgaatgg
       61 ctggccaagg cgatgggcgt tcgcgacgtc acgatcgccc tcttctcggt caccctgctg
      121 atcctgctcg ccctctcgct caacttttcg gtgatcgctt cccgccagtc gaagcagatc
      181 acacggctcg cccagcaaca ggcgatcgac ggggtcgagg aagaagcggc tagtcccgca
      241 gcgtcttctc gaggcgaaac gccagcgcct cgagagcaag ctcctcgctg acgttcagct
      301 ggaagctgcg gcgggtttcc tggatctggc tgaccgcgtc acgggcctgg ccgagttcga
      361 tcccttccgc ctgggcctcg aggacttcga gccggtcctg gttgaaggcg aggtcggggg
      421 cgccggcgat gaccgtcgcc cagtcacggg cccaggcggc ggcgaggtga aggccgaggt
      481 cgagcatgcc ggtgcgggtc ttccgctggg cccttcgcac cgcctcctcg atctcggtct
      541 tggtgtgctt gatgccctct tccttttcct cgtcgaagtc ggcctggacg agctcgccgg
      601 aatgctcgcc cgccgacctg gccttggcca gcaggtcaag ccagggcgag ccggcgaact
      661 cgtcttcgag ggcgcaggtc atcagccgtt cggccgcgac cctgaggtcg ctgccccgtt
      721 tgccggccag aaaccttgcc ttgtcgaggt ccccgccggc cagtttggcg gccgagtgga
      781 gcgcctcggg acttgccagc ccgtccagtt ccgcttcaat cacctcgaac gggagcgacc
      841 cgaactcgat cagctggcag cgggaggcga tggtcggcag caggccttcg ctttcggctg
      901 aaagcaggat caggtgggca tgcggcgccg gctcttcgag ggtcttcagc atcgcgttct
      961 ggctttcctc gttcagggcg tcggccgcct cgatcacgaa gacccgggcc gagccctcga
     1021 aggggctgag cggcgcctgg tggatgatcc ggtcgcggat ctcctggacc gcgtggctca
     1081 tgcctttcgg ccgcagccag accaggtccg gatggggcga ggggtcaagc agggcgcgcc
     1141 ggcgggagtc ctcggggtcg gcgctgccgg cgctcagtat cccggccgcg aaagcccggg
     1201 cggccctggc cttgccggac ccggagggac cccggaagag ataggcatgc gatggcccct
     1261 gcctgagagc ggcttcgagc tcggcccgga cccggggctg atgggaggtg gattcctcca
     1321 gcgtgatcac gccagcatcc tcgcaagccg cgaggaggcc acgccgcctc cccgcccttt
     1381 cacgccggga gcctggcttc gacctcggcc aggatcagcc ggtggacctg ctcgggggat
     1441 ccggtcgcgt caatcggtac gacccggttc ggctcgcgac gggcgatgtc ttcgtaggcc
     1501 tcggcgaccc gttcctgaaa acgaacccct tccgcctcga agcggtcgtc ggacccggcc
     1561 cggtccagcc cgaa
//
LOCUS       DAOQFI010000571         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_54807_length_1571_cov_2.432718, whole genome shotgun sequence.
ACCESSION   DAOQFI010000571 DAOQFI010000000
VERSION     DAOQFI010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54807_length_1571_cov_2.432718"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLE93_09370"
     CDS             complement(<1..546)
                     /locus_tag="PLE93_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH87300.1"
                     /translation="MDGPPVVFVSNCDLLSRPGSQQAGVDIELAGGTVRRIGPTGESP
                     APAGAELIDGTGLLAMPGLVNAHSHSPENCLRGVGEGLRLEPWLLRMFGSSGLYSPED
                     HYVNALAGAVEMLLLGVTTVVDHLWMTPPSLDAVEGAVRAYEEAGIRAAVAPLMNDCD
                     FTGELAAASDFDLGPALMSEQV"
     gene            complement(774..>1571)
                     /locus_tag="PLE93_09375"
     CDS             complement(774..>1571)
                     /locus_tag="PLE93_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNH87301.1"
                     /translation="QEAGEKPAKYQVPAVVGAISVLHEVATSDEPGVTQAELVKSTGL
                     SKSTMHNLLATLEAHDFVRRENNSRNYRLGPALIPLGTEASRQVRLVRSTIDRVAPLA
                     AEYHLSFAVAQVVSDEECRIIERFYPTEDVHVGILIGSSYGPMDGALGKVLLAYMDPA
                     KARRIVKSRKLPAHTDATITSPENLIETVEEAKVRGWAISAGELNQNHAVATGVLGQN
                     GELALLLLALGFPADLDDDRMGEIGQLLAGIAASIMADAGISARSEA"
BASE COUNT          263 a          517 c          532 g          259 t
ORIGIN      
        1 gacctgttcg gacatcaagg ccggcccgag atcgaagtcg gatgcggcgg caagctcgcc
       61 cgtgaagtcg cagtcgttca tgagcggcgc gaccgcggcc cggatcccgg cttcctcgta
      121 ggcgcgaacc gcaccctcga ccgcgtcgag cgagggcggc gtcatccaga ggtggtcgac
      181 caccgtggtg accccgagca acagcatctc gaccgccccg gcaagcgcgt tcacgtagtg
      241 gtcctcggga ctgtagagac cggaagaacc gaacatccgc aggagccaag gctcgaggcg
      301 caggccctcg ccgacgcctc gcaggcagtt ctccggggag tgcgagtggg cgttgaccag
      361 gcccggcatg gccagcagcc cggtgccgtc gatcagctcg gcgcctgccg gagcgggtga
      421 ctcaccggtc ggaccgatcc gacggacggt gccccccgcc aactcgatgt ctacgccggc
      481 ctgctgcgag ccggggcgtg agagcaggtc gcagttgctg acgaaaacga cgggcgggcc
      541 atccacgcgg tgcaactcga caagcctcct gtggggagcg gtcaccggct cctctcttcc
      601 cgtgacctgg ggcaaacaaa gttcaataac ttgaactcag ttctaggaat gaaacatagc
      661 accggctatc gaaacttgca aagaaatctc gatatccgtt cgcgcaggac gacttcccgg
      721 ctgttcggcc ggcctctcga cgggtggagg gaagcggtct tacgaggggt gagtcaggct
      781 tcggagcggg cgctgatccc cgcgtcggcc atgatcgacg cggcgatccc ggcgagcagc
      841 tggccgatct cgcccatgcg gtcatcgtcg aggtcggccg ggaaaccgag cgcgagcaga
      901 agcagggcga gctcgccgtt ctgaccgagc acaccggtcg ccacggcgtg attctgattc
      961 agctcgccgg cgctgatcgc ccagccgcgg accttcgcct cttcgaccgt ctcgatcagg
     1021 ttctcggggc tggtgatggt cgcgtcggta tgcgccggca gctttctgga cttgacgatc
     1081 cgtcgtgcct tcgccgggtc catgtaggcc agcagcacct tgcccagggc gccgtccatc
     1141 ggcccgtagg aggaaccgat caggatcccg acgtggacgt cctcggtcgg gtagaagcgt
     1201 tcgatgatcc ggcactcctc gtcgctgacc acctgggcca ccgcgaacga gaggtggtac
     1261 tcggcggcca gcggcgcgac ccggtctatg gtcgagcgga ccagccggac ctgacggctg
     1321 gcttcggtcc cgagcgggat caaggcgggg ccgagccggt agttgcggga attgttctcg
     1381 cgccgcacga agtcgtgggc ctcgagggtc gccagcaggt tgtgcatggt cgacttggac
     1441 aaaccggtgg acttgaccag ctccgcctgc gtgaccccgg gctcgtcgct cgtggctact
     1501 tcatgcagca cgctgatcgc cccgaccacg gccggaacct gatacttggc cggcttctcg
     1561 ccggcttcct g
//
LOCUS       DAOQFI010000572         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_54943_length_1569_cov_2.602378, whole genome shotgun sequence.
ACCESSION   DAOQFI010000572 DAOQFI010000000
VERSION     DAOQFI010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54943_length_1569_cov_2.602378"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLE93_09380"
     CDS             complement(<1..358)
                     /locus_tag="PLE93_09380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH87302.1"
                     /translation="MAGSKGKQFSRFVAIGDSQTEGVGDPTGPGGLERGWADRFAERL
                     DLTRRATGEAGLLYANLAVRGKLLAQIDEDQIEPALAMEPDLVSLIGGLNDVIRPGCD
                     IDLVLARMDTMQGRLAA"
     gene            complement(399..>1569)
                     /locus_tag="PLE93_09385"
     CDS             complement(399..>1569)
                     /locus_tag="PLE93_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH87303.1"
                     /translation="VLKGKWHLTQPVGSDWSQADAERLADEYGFEGWVPPDAGENIEP
                     SHFGGGNKAGKSKEGYDEDFTRQAEEFLADPPDEPWALIFSLVNPHDVLGYPSSYEEG
                     GYSDREWADLDAVQLPPSVDETMKNKPRVHSLLKIGQLSFLGPIEGEQRLEYCRFYAH
                     LHRLADAKIGRVLEALGDPEDPDSLRSKTVIFRTSDHGDLGMSHGGLRQKMFNAYEET
                     VNVPLIVSNPQMFPEARRTNAPASLCDVLPTMAELAGADTEGLELRGRSLVPVLKEEA
                     ESVQEGTLFTYDDHQSGTAYRDVTPGANRIRAWRTPEATYAIYLNPHGGPPEFELYDR
                     TRDPDQVDNLVDRDSGRAINPADRELLEKMRAGLKAEMRRCRVPALPATIDRLPA"
BASE COUNT          231 a          512 c          547 g          279 t
ORIGIN      
        1 tggcggcaag tcgaccctgc atcgtgtcca tccgggcgag gacgaggtcg atgtcgcaac
       61 cgggccggat cacgtcattg agaccgccga tcaggctgac caggtccggt tccatcgcca
      121 gggccggctc gatctggtct tcgtcgatct gggcgagcag ctttccccgc accgcgagat
      181 tcgcgtagag cagccccgct tcgccggtgg cccgccgggt caggtcgaga cgctcggcaa
      241 agcggtccgc ccagccccgt tcgagacctc ccggcccggt cgggtcaccg accccctcgg
      301 tctggctgtc cccgatcgcc acaaaacgac tgaactgctt ccctttgctg cccgccatgc
      361 cgaaacccta tcccccaagc ggaggaattc aagggcggtc aggccgggag gcggtcgatc
      421 gttgccggca gggccgggac gcggcagcgg cgcatttcgg ccttaagacc ggcacgcatc
      481 ttctccagca gttcgcgatc ggccggattg atcgcccggc cgctgtcgcg gtcgaccagg
      541 ttgtcgacct gatccgggtc ccgggtgcgg tcatagagct cgaattccgg ggggccgcca
      601 tgcggattga ggtagatcgc ataggtcgcc tccggggtac gccaggcgcg gatccggttg
      661 gcgcccgggg tgacgtcgcg gtaggcggtg ccggactgat ggtcgtcgta ggtgaagagg
      721 gtcccttcct ggaccgactc ggcctcctct ttcagcaccg ggaccaggct gcggccgcgg
      781 agctcgagcc cttcggtgtc ggcgccggcc agttccgcca tcgtcggcag cacgtcgcag
      841 agcgacgcgg gggcgttggt ccgccgggcc tcggggaaca tctgcgggtt ggacacgatc
      901 agcggcacgt tgacggtctc ctcgtaggcg ttgaacatct tctgacggag gccgccgtgc
      961 gacatgccga ggtcgccgtg gtcagaggtg cggaagatca ccgtcttcga acggagtgaa
     1021 tccgggtcct cgggatcacc cagcgcctcc aggacgcggc cgatctttgc gtcggcgagg
     1081 cgatgcaggt gggcgtagaa acggcagtac tcgagacgct gctcgccttc gatcgggccg
     1141 aggaagctga gctggccgat cttcagcagg gaatgcaccc ggggcttgtt cttcatggtt
     1201 tcgtcgacgc tcggcggcag ctggacggcg tcgaggtcgg cccattcgcg gtcgctgtag
     1261 ccgccctcct cgtacgagga ggggtaaccg agcacgtcgt gaggattgac gagcgagaag
     1321 atcagcgccc agggctcatc gggcgggtcg gcgaggaact cctcagcctg gcgggtgaag
     1381 tcctcgtcgt agccttcctt gctcttgccg gccttgttgc cgccgccgaa gtggctcggc
     1441 tcgatgttct ctccggcatc cggcggcacc cagccctcga agccgtattc gtcggcgagg
     1501 cgctcggcgt cagcctggga ccagtcgctg ccgaccggct gggtcagatg ccacttgccc
     1561 ttgagcacg
//
LOCUS       DAOQFI010000573         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_55465_length_1562_cov_2.483079, whole genome shotgun sequence.
ACCESSION   DAOQFI010000573 DAOQFI010000000
VERSION     DAOQFI010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55465_length_1562_cov_2.483079"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PLE93_09390"
     CDS             <1..1181
                     /locus_tag="PLE93_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87304.1"
                     /translation="AELTVIPSSSDVANLTAVDQFGIGMRLATFNASGTKMETVGSAN
                     SDTIFNALQKIPGGPGATIKNSSGRIIRVLSPNKSDSYPQLGQYVKSMAGRKITLNTA
                     FYGDPFTTSQYSGTFASDGSITLNGQTNPPGAAPAQLQFPGAQVTADIYTGGNTPNNL
                     SGALYRDLLAGFSTGLWGGKYGNDAISFCSNPIINDQGKWCPSGFNKPAFGDARKSLS
                     SYPACEQYAAIINRYSDSYGNPYSDASKKVTVGLDQPATGGSVTTLQLTVLPDSGDAG
                     PAQSGNANCGAAPKPKVKKVSFYAKAKLKGNSAKVGRVTCTVACGQIKVVAKKNGKKV
                     AYRKYKVTGKKPYMTMKVTKAGRKMFHRHKKLTVKVQVQIKPAGASQVTRKHSLKFIR
                     "
     gene            1211..>1562
                     /locus_tag="PLE93_09395"
     CDS             1211..>1562
                     /locus_tag="PLE93_09395"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87305.1"
                     /translation="MGKSGKPKKLDKKGYEEELLRLQGELVQLQEWIVNQGLKVIVVF
                     EGRDTAGKGGVIKRITERTSPRIIRTVALGTPSDKEKTQWYFQRYVAHFPAAGEMVLF
                     DRSWYNRAGVERVMG"
BASE COUNT          357 a          502 c          468 g          235 t
ORIGIN      
        1 gggccgagct gacagtgatt cccagctcct ccgacgttgc caacctgacc gcggtcgatc
       61 aattcggaat cggcatgcgg ctcgcaacct tcaacgcatc cggcacgaag atggaaaccg
      121 tcggttcggc caactccgac acgatcttca acgccctgca gaagatcccc ggcggcccgg
      181 gcgcgaccat caagaactcc agcggcagga tcatccgggt cctgtcaccc aacaagagcg
      241 actcgtaccc gcaactcggc cagtacgtca agtcgatggc gggcaggaag atcaccctca
      301 acaccgcctt ctacggagac ccgttcacga ccagccagta ctcgggtacc ttcgcctcgg
      361 acggctcaat cacgctcaac ggccagacca acccgcccgg ggccgcaccg gcccaactcc
      421 agttcccggg agcgcaggtg accgctgaca tctacaccgg cggcaatacc ccgaacaacc
      481 tttccggggc gctctaccgg gatctgctgg ccggattctc taccggcctg tggggcggca
      541 agtacggcaa cgacgcaatc tccttctgct cgaatccgat catcaacgac caaggcaagt
      601 ggtgtccgag cggattcaac aagcccgcct tcggtgatgc ccgcaagtcc ctttcctcct
      661 acccggcatg cgagcagtac gccgcgatca tcaacaggta ctccgattca tacggcaacc
      721 cctacagcga cgcttcaaag aaggtcaccg tcggtctcga ccagccggca accggcggca
      781 gcgtcacgac cctccagctg accgtacttc cagacagcgg ggatgccggg cccgcgcaaa
      841 gcggcaatgc caactgcggc gccgcgccga aaccgaaggt caaaaaggtc agcttctacg
      901 ccaaggcgaa gctcaagggc aactcggcga aagtgggtcg ggtcacctgc acggtcgcct
      961 gcggacagat caaggtagtg gcgaagaaga acggcaagaa ggtcgcctac cgcaaataca
     1021 aggtgaccgg aaagaagccg tatatgacca tgaaggtgac aaaggctggc cggaagatgt
     1081 ttcacaggca caagaagctc acggtcaagg tgcaggtcca gattaagccg gccggcgcaa
     1141 gccaggtgac gcggaagcat tcgctcaagt tcatccgctg atccaggggc gcgagccata
     1201 tgcttcgcca atggggaagt ccgggaaacc gaagaagctg gacaagaagg gctacgagga
     1261 ggagttgctc cgcctgcagg gtgagctggt ccagctccag gagtggatcg tcaaccaggg
     1321 cctgaaggtg atcgtggtct tcgagggtcg cgatacggcc ggcaagggcg gcgtgatcaa
     1381 gcgaatcacc gagcggacca gcccgcggat aatccgcacg gtcgctctcg gcacaccctc
     1441 ggacaaggaa aagacgcagt ggtatttcca gcggtacgtg gctcacttcc cggcggccgg
     1501 tgagatggtc ctcttcgacc gcagctggta caaccgcgcc ggcgtcgagc gggtgatggg
     1561 ct
//
LOCUS       DAOQFI010000574         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_55481_length_1562_cov_2.025216, whole genome shotgun sequence.
ACCESSION   DAOQFI010000574 DAOQFI010000000
VERSION     DAOQFI010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55481_length_1562_cov_2.025216"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PLE93_09400"
     CDS             complement(<1..1081)
                     /locus_tag="PLE93_09400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNH87306.1"
                     /translation="MALGGAEVAARESGSPTAKAAGSNFRPDIQGIRAIAVTLVLACH
                     ASIPWAKGGFIGVDVFYVLSGFLITGLVVREIEKTGKVSIRNFYARRAKRLLPMAALV
                     LVFICIGTLFIFSLARQVEIGGDVFAAALYFVNWHFIIQGVDYFAAADGIHSPVQHYW
                     SLSVEEQFYVVWPLILLAAGALAAKFRRPTKGLVLIFIVPVAIASLIYSIHFTQVNPE
                     AAFFSTIARAWQLAFGAVLAMILPKKIGMPAWLATVIGVIAVGVLGWATYTMSASTPF
                     PGSNGLYPVGATIALLVVGASGAGITTRILSTRPFQYLGNISYSWYLWHWPFVVFALG
                     IFDNLNWHWLVVATLISWIPTQISHT"
     gene            complement(1134..>1562)
                     /locus_tag="PLE93_09405"
     CDS             complement(1134..>1562)
                     /locus_tag="PLE93_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87307.1"
                     /translation="SPEVAAAYRTDYDHARSATGSPTHLQAKTASHSTGERYTAPSVI
                     FEYGDKRAEVGGFQPVEAYDVMIANIEPGLKRMGPAEDPLEALGLYPAGLTTQEVAAI
                     MASGMDKPERVDAERKLVHLLGERKVKRVPLGDDALWIKA"
BASE COUNT          297 a          466 c          544 g          255 t
ORIGIN      
        1 gggtgtggct gatctgggtc gggatccagg agatcagggt ggcgacgacc agccagtgcc
       61 agttcaggtt gtcgaagatg ccgagcgcga agacgacgaa gggccagtgc cacagatacc
      121 aggaatacga gatgttgccg aggtactgga agggccgggt cgagaggatc cgggtggtga
      181 tgccggcacc ggaggcaccg accacaagca gggcgatcgt cgccccgacc gggtagaggc
      241 cgttggaacc ggggaatggg gtggaagccg acatcgtgta ggtcgcccag ccgagcaccc
      301 cgaccgcgat caccccgatc acggtggcca gccaggccgg catgccgatc ttcttcggca
      361 ggatcatcgc cagcaccgcg ccgaaggcga gctgccaggc ccgggcgatg gtcgagaaga
      421 aggccgcctc ggggttcacc tgggtgaagt ggatgctgta gatcagcgag gcgatcgcga
      481 ccgggacgat gaagatcagg accaggccct tggtcggacg gcggaacttc gcggccaggg
      541 caccggcggc cagcaggatc agcggccaga ccacatagaa ctgctcttcg accgacagcg
      601 accagtagtg ctggaccggg ctgtgaatgc cgtccgcggc ggcgaaataa tcgacgccct
      661 ggattatgaa atgccagttg acgaagtaga gcgcggcggc gaatacatct ccgccgattt
      721 cgacctggcg ggccagcgag aagatgaaca gggtgccgat gcagatgaag acgaggacca
      781 gcgcggccat cggcaagagg cgcttggcgc ggcgggcata gaagttcctg atcgagacct
      841 tgcccgtctt ctcgatctcg cgaacgacca ggccggtgat caggaagcct gagagaacgt
      901 agaagacatc gaccccgatg aatccaccct tcgcccaggg gatcgaagcg tggcatgcaa
      961 ggacaagggt gaccgcaatc gcgcggattc cctggatatc gggccggaag ttcgaacccg
     1021 ctgcttttgc cgtgggggag ccgctttctc tggcggcgac ctcggcaccg ccaagagcca
     1081 tcggcgcagg ctacaggttt ggggtgacgc cccgctcgct cagcgcgggt acctcaagcc
     1141 ttgatccaga gggcgtcgtc gcccagcgga acccgtttca cttttcgctc tccgagcagg
     1201 tgaaccagct ttcgttcggc gtccacgcgc tcgggcttgt ccatcccgga agccatgatc
     1261 gctgcgacct cctgagtggt caggccggcc gggtaaagtc cgagcgcctc cagcggatcc
     1321 tcggccggac ccatgcgttt caggccgggt tcgatgttgg cgatcatcac gtcgtaggcc
     1381 tccactggct ggaagccgcc cacttcggct cgcttgtcgc cgtattcgaa gatcaccgag
     1441 ggtgccgtat acctttcgcc ggtcgagtgg gaagcggtct tggcctggag gtgggtcgga
     1501 ctcccggtcg cgctgcgggc gtggtcgtaa tcggtccggt aggcggcggc cacttccggg
     1561 ct
//
LOCUS       DAOQFI010000575         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_55510_length_1561_cov_3.189907, whole genome shotgun sequence.
ACCESSION   DAOQFI010000575 DAOQFI010000000
VERSION     DAOQFI010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55510_length_1561_cov_3.189907"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLE93_09410"
     CDS             complement(<1..244)
                     /locus_tag="PLE93_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH87308.1"
                     /translation="MSALVQMRGIVKRFGDFTASREVDFDVAPGEVHALLGENGAGKT
                     TLMNILYGLVQADEGTIEVDGREVDLKGPADAIEAGV"
     gene            complement(318..1337)
                     /locus_tag="PLE93_09415"
     CDS             complement(318..1337)
                     /locus_tag="PLE93_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HNH87309.1"
                     /translation="MRIGKILSAAAACAVLATGVAACGSDDNGGSSGDSKLKVAMVLP
                     GPINDKGFNQSGYDGLKQCEADGAEINYQEKTPVPDFEKAFETLAQSNDMVIGHGFEF
                     GEIAHKVAPDFPDTDFVVTSNPLEPEDSNVQHLMPNSTQGAYLAGVAAGKTTTSNKLG
                     GIAGFDFPVLEQQMKAVEAGAQSVNPKATLETVYLGTFDDVAKGKEAARSQAASGVDV
                     IYHIADAAGVGVIDGAEEEGIKAIGWGVDQSALAPKTIIASQLVDQAKMISDSCQEAI
                     DGKWKGGLVVDGLQSGLVDLTPVKNAPAGTQEAVDKARAAIIDGSAEVPSIGGTIPGS
                     GEGSN"
     gene            complement(1340..>1561)
                     /locus_tag="PLE93_09420"
     CDS             complement(1340..>1561)
                     /locus_tag="PLE93_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87310.1"
                     /translation="HVIVDGRVVVRDGNLATADGAAVREALREQVQRRSREVASPPQG
                     TGEAMERIETFIRSLGDSAATVSNQNRRS"
BASE COUNT          214 a          551 c          485 g          311 t
ORIGIN      
        1 cgacgcccgc ttcgatcgca tccgcgggcc ccttcaggtc gacctcgcgg ccgtcgacct
       61 cgatcgtccc ctcgtcggcc tgcaccaggc cgtagaggat gttcatcagg gtcgtcttgc
      121 ccgctccgtt ctctccgagc agggcgtgga cctctccggg agcgacgtcg aagtcgacct
      181 cgcgactggc ggtgaaatca ccgaaccgct tgacgatccc ccgcatttga accagtgccg
      241 acactcaaca acctcctccc ccgggggcgg tcgcccccgg gggtcttctc gtcttcgcta
      301 cgcgttcaac ccgtaactca gttcgagcct tcgcccgaac ccgggatcgt tccgccgatg
      361 ctcggaacct cggcggagcc gtcgatgatg gccgcgcggg ccttgtcgac cgcttcctgg
      421 gttccggccg gagcgttctt gaccggggtc aggtcgacga ggccggactg caggccgtcg
      481 accaccaggc cgcccttcca cttgccgtcg atcgcctcct ggcaggagtc gctgatcatc
      541 ttcgcctgat cgacgagctg cgaggcgatg atggtcttcg gggcgagggc gctctggtcg
      601 acaccccagc cgatcgcctt gatgccttcc tcttcggcgc cgtcgatcac gccgactccg
      661 gcggcgtcgg cgatgtggta gatcacgtcg actccggaag cggcctggct gcgggcagct
      721 tccttgccct tggcgacgtc gtcgaaggtg cctaggtaga cggtctcgag cgtggccttc
      781 gggttgaccg actgggcccc cgcttcaact gccttcatct gctgctcgag caccgggaag
      841 tcgaatccgg cgatcccgcc gagcttgttc gaggtggtcg tcttgcctgc cgcgacgccg
      901 gccaggtacg cgccctgggt cgagttcggc atcagatgct ggacgttgga gtcctccggt
      961 tcaagcgggt tcgaggtcac cacgaagtcg gtgtccggga agtcgggcgc caccttgtgg
     1021 gcgatctcgc cgaactcgaa gccgtggccg atcaccatgt cattgctctg ggccagcgtc
     1081 tcgaacgcct tctcgaagtc ggggaccggg gtcttctcct ggtagttgat ctccgccccg
     1141 tcggcttcgc attgcttcag cccgtcgtac ccggactggt tgaatccctt gtcgttgatt
     1201 ggaccgggca gcaccatcgc gaccttgagc ttcgagtcgc cggatgagcc tccgttgtca
     1261 tcggaaccgc aagcggccac tcccgtcgcc agcaccgcgc acgcggcggc agcgctgaga
     1321 atctttccta tacgcacctc taactcctcc tgttttggtt ggacacagtg gccgccgagt
     1381 cccccagcga ccggatgaaa gtctcgattc gctccattgc ctcgcccgtg ccttgcggcg
     1441 gcgaggcgac ctcgcggctg cggcgctgaa cctgctccct cagggcctcc ctgaccgccg
     1501 cgccgtcggc cgtggccagg ttgccgtcgc gaacgacgac ccgtccgtcg acgatcacgt
     1561 g
//
LOCUS       DAOQFI010000576         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_55864_length_1556_cov_2.059960, whole genome shotgun sequence.
ACCESSION   DAOQFI010000576 DAOQFI010000000
VERSION     DAOQFI010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55864_length_1556_cov_2.059960"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLE93_09425"
     CDS             <1..358
                     /locus_tag="PLE93_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNH87311.1"
                     /translation="QSGHIISTDYAPTGDGIAAALMLLQALDGRELADARVMEKLPQV
                     LVNVEVADREAIAGAEPVWTEVDRLNAELEGKGRVLLRPSGTEPLVRVMAEAPSQEQA
                     QEICDSLAGLVRSELG"
     gene            421..>1556
                     /gene="glmS"
                     /locus_tag="PLE93_09430"
     CDS             421..>1556
                     /gene="glmS"
                     /locus_tag="PLE93_09430"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNH87312.1"
                     /translation="MCGIIGYVGNRPAEEILVAGLQKLEYRGYDSAGVAILAPEEGVK
                     KVRAVGNLDGLRSALQQTDLSSTDEADAEGHFTGIAHTRWATHGRVTEANAHPHGDES
                     GNVQIVLNGIVENYVKLKSRLQEDGVTFTSETDAEVVAHLIHRYYEGDLTEAVRKAYA
                     DLRGHYSIVALHSEAPGVLVGARKETPLIVGVGEHENFIASAIPAFLNETRLALTIDD
                     GEIVTLTADGVTVTKADGEPVERATEEVEWDEETAEKAGYDTFMMKEIHEQPEAIAET
                     ILDRLTEDDSVDLSDIGFGDEVFRQCSRIVIVACGTSYHAGLVGRYAIERWARVPVEM
                     DIASEYRYRDPVIGEDDLVIGITQSGETADTLAAMRLARDRGAKV"
BASE COUNT          286 a          501 c          513 g          256 t
ORIGIN      
        1 gcagtccgga cacatcattt cgaccgatta cgccccgacc ggggacggga tcgccgccgc
       61 cctgatgctg cttcaggccc tggacggtcg cgagctggcc gatgcccggg tgatggagaa
      121 gctgccccag gtcctggtta acgtcgaggt ggcagaccgc gaggcgatcg ccggggcaga
      181 gccggtctgg accgaggtcg accggctgaa cgccgaactc gaaggcaagg gccgggtgct
      241 gctgaggcct tccggcaccg agccgctggt ccgggtcatg gcggaggctc ccagccagga
      301 gcaggcacag gagatctgtg actcgctggc cgggctggtc cgctcggaac tcggctgatt
      361 tctccctccg tgaccgatct ctccccggcg gcgcgaccgc atggctaggc tttagcgctc
      421 atgtgcggaa tcatcggata tgtcggcaac cggcccgctg aggagatcct cgtggcgggt
      481 ctccaaaagc tggagtaccg tgggtacgac tctgcagggg tggcgattct cgctcccgag
      541 gagggtgtga agaaggtccg ggcggtcgga aacctcgacg ggctgcggag cgctcttcag
      601 caaaccgacc tgtcttcgac ggatgaagcc gacgccgaag gtcatttcac cggcatcgcc
      661 cacactcgct gggcgaccca cggccgggtg accgaggcca atgcccatcc ccatggtgac
      721 gagtccggca atgtccagat cgtcctcaac gggatcgtcg agaattacgt gaagctgaag
      781 tcccgcctgc aggaggatgg ggtcaccttc acctccgaaa ccgacgccga agtagtcgcc
      841 catctgattc accgctacta cgagggtgac ctgaccgaag cggtccgcaa ggcctacgcc
      901 gatctgcgcg gccactactc gatcgtcgcg ctccattcag aagctcccgg cgtgctggtc
      961 ggggctcgca aggagacgcc gctgatcgtc ggagtcggcg agcacgaaaa tttcatcgca
     1021 tccgcgatcc ccgccttcct caacgaaacc cgcctcgccc tgaccatcga tgatggcgag
     1081 atcgtcaccc tgaccgctga cggcgtcacc gtgaccaagg ccgatggcga gccggtcgag
     1141 cgggctaccg aagaggtcga gtgggatgag gagaccgccg agaaggccgg atacgacacc
     1201 ttcatgatga aggagatcca cgagcagccg gaagcgattg ctgagaccat acttgaccgt
     1261 ctgaccgaag acgacagtgt ggatctttcc gatatcggtt tcggcgacga ggtcttccgc
     1321 cagtgctcgc ggatcgtgat cgtcgcctgc ggcaccagct accacgccgg tctggtcggt
     1381 cgttacgcga tcgagcgctg ggcccgggtg ccggtcgaga tggacatcgc ctccgagtac
     1441 cgctaccgcg acccggtgat cggtgaagac gatctggtca tcggcatcac ccagtccggc
     1501 gagaccgccg acaccctggc cgcgatgcgg ctggcccgcg accgcggcgc caaggt
//
LOCUS       DAOQFI010000577         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_56327_length_1549_cov_2.908300, whole genome shotgun sequence.
ACCESSION   DAOQFI010000577 DAOQFI010000000
VERSION     DAOQFI010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56327_length_1549_cov_2.908300"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /gene="priA"
                     /locus_tag="PLE93_09435"
     CDS             <1..997
                     /gene="priA"
                     /locus_tag="PLE93_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNH87313.1"
                     /translation="PASGRERVEVRTWEALAEVREKGEKAIVMINRRGFAPWLTCQTC
                     GHHWGCPNCDVSLIVHRESDQLICHHCNHAEPLPRRCPDCGGTTLSQTGAGTQRIERL
                     LAEELAPMPVFRLDADTSAGPGGHATILSAFDQEESAILVGTQMVAKGHDFPEVTLAA
                     ILDADATLRFPDFRAEERTFSLVTQLAGRSGRSRTGGKVIVQTLAPGSPSISHAATHD
                     SAGFLAGELDRRRELGYPPFSHLIRIQLAAEAEPDVERAANLVAARLDDALPAGAEML
                     GPAPMFRARNRYRRRLLVRSTTRAESIAAVHSTIEELLADKSLRKITLAIDVDPQ"
     gene            1081..>1549
                     /locus_tag="PLE93_09440"
     CDS             1081..>1549
                     /locus_tag="PLE93_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNH87314.1"
                     /translation="MASEDETATLSDEAAAYAEGMERAEEALAEAGADGAAGEEVEVE
                     PEGGEEPVRHYDEETLRRREAALARIVQYGDPMLKSRSSEVTDFDQALTDEIERMFEL
                     MKDGLGVGLAAPQAGKLRRLLVFQTNADAEPQELVNPDIEWLSEGDSPSGRRVS"
BASE COUNT          268 a          536 c          525 g          220 t
ORIGIN      
        1 ccccgccagc ggccgcgagc gcgtggaagt gcgcacctgg gaagccctgg ccgaggtgag
       61 ggagaagggc gagaaggcga tcgtgatgat caaccgccgg ggctttgcgc cctggcttac
      121 ctgccagacc tgcgggcacc attggggctg cccgaactgt gatgtctcgc tgatcgtcca
      181 tcgggagtcg gaccagctga tctgccacca ctgcaatcac gccgaaccgc tgccccggcg
      241 ctgccccgac tgcggcggga cgacgctctc gcagaccggg gccgggaccc agcggatcga
      301 acggctgctg gccgaggagc tggctccgat gccggtcttt cgccttgacg ccgacaccag
      361 cgccggtccg ggtggccacg cgacgatcct cagtgccttc gaccaggagg agagtgcgat
      421 tctggtcggc acccagatgg tcgccaaggg gcacgacttc cccgaagtga ccctcgccgc
      481 gatcctcgac gccgatgcca ccctccgctt ccccgacttc cgggccgagg agagaacctt
      541 ttcgctggtc acccagctgg ccggccgcag cggccgcagc cggaccgggg ggaaggtgat
      601 cgttcagacg ctggctccgg gcagcccctc gataagccac gccgcgaccc atgattccgc
      661 cggctttctg gccggcgagc tcgatcgacg ccgcgaactg ggctatccgc cgttttccca
      721 tctgatccgc atccagctcg cggccgaagc cgagccggat gtggagcggg ccgcgaacct
      781 ggttgccgcc cgcctcgacg acgcccttcc cgccggagcg gaaatgctcg gcccggcccc
      841 gatgttccgg gcacgaaacc gctaccgccg ccgcctcctg gtccgctcga cgaccagagc
      901 cgaatcgatc gcggcggtcc acagcacaat cgaggaactg ctcgccgaca aatccttgag
      961 gaagatcacg ctggcaatcg acgtcgatcc ccagtgatca ggcgcagcag gcaggctcgc
     1021 cccttgacgg gggtttccac ggctcctgcg tgattccggg cgtcggtaga ctcggcgacg
     1081 atggcttccg aagatgaaac cgccaccctg agcgacgaag cggccgcata tgccgagggc
     1141 atggagcggg ccgaagaggc gctggccgag gccggcgccg atggggctgc cggggaagaa
     1201 gtggaagtcg agccggaggg cggcgaagag ccggtccgcc actacgacga agagacgctc
     1261 cgccggcgcg aggctgcgct ggcccgaatc gtccagtacg gcgacccgat gctgaagagc
     1321 cgctcctcgg aggtgaccga cttcgaccag gccctgaccg acgagatcga gcggatgttc
     1381 gagttgatga aggacggtct cggggtcgga ctggcggcac cgcaggccgg caagctgcgc
     1441 cggttgctcg tcttccagac caatgccgac gcggagccgc aggaactcgt caaccccgac
     1501 atcgaatggc tttccgaagg cgacagtccc agcggacgac gcgtctcgc
//
LOCUS       DAOQFI010000578         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_56473_length_1547_cov_2.615282, whole genome shotgun sequence.
ACCESSION   DAOQFI010000578 DAOQFI010000000
VERSION     DAOQFI010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56473_length_1547_cov_2.615282"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLE93_09445"
     CDS             complement(<1..349)
                     /locus_tag="PLE93_09445"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNH87315.1"
                     /translation="MGLFSGVEQDSVDKRFSIRGPQEPNQKVTTVLIDAETFDQLNEQ
                     WPFPRSLHGNAIDRLRKAGAKVIAYDVQFTEPTIPREDNRLLSAVRRAGNVALATDAV
                     YLNGTSDVFGGVET"
     gene            complement(605..>1547)
                     /locus_tag="PLE93_09450"
     CDS             complement(605..>1547)
                     /locus_tag="PLE93_09450"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87316.1"
                     /translation="GQLACWGKGAFGVLGDGTTADRTTPAPVTGIASAERQTLAVVKK
                     GEGTGTISSIPAGINCGSTCSGTFSPGAAVTLTTSAGPGSAFTGWSGACAGSGSTCQV
                     TMNQARVAIATFRKNPVVPTSYKLTVTKAGTGAGTVASNPVGINCGSDCEESYGPGTM
                     VTLIAAAKPGSTFQGWSGPCSGIRACQVTMSESRNVTATFSSVQSEGFRGRIAVTPKY
                     QVIHRGDWEYFRVQIESTNGVPASNVNVCPSSEWAALWFSGCAQLGNVPGGGSKSGLI
                     KVGLYSWASLGYDRAVAFTFSSPTFGSGTKEAIVVAR"
BASE COUNT          318 a          524 c          416 g          289 t
ORIGIN      
        1 gtgtctcaac tcccccgaag acgtcgctgg tcccgttcaa gtacacagca tcggtggcca
       61 gtgccacgtt accggcgcgt cgcaccgccg aaagtaaccg gttgtcctcc cgcgggatcg
      121 ttggttcggt gaactgaacg tcatatgcga tgactttcgc ccctgctttg cgcagacgat
      181 caatcgcgtt gccgtggagt gacctgggaa acggccattg ttcgttcagc tgatcaaagg
      241 tttccgcatc gatcagcacc gtcgtcacct tctggttggg ttcctgcggt cctcgaatcg
      301 agaatcgctt gtcgaccgag tcctgttcaa ccccgctgaa cagccccacc ccccagacca
      361 ggatcccgat tgccgaagca atcacggcaa cgacgatgag cgcggaccgt cgtcggttca
      421 tccgctcccc aggaccgact tctcaagact ggtcttcacc aggacagctc cctcctgagg
      481 cgtcattgcc tcgtcaagtc aagttagcag cgaataccca cgcccgcaac gccgggaccc
      541 ggtcgccgac cgggtcccga atcctccctt tcggaattcg aatttgacaa tcccgggttc
      601 acgactacct ggcaacaaca atggcttcct tggtcccgct accaaaggta ggactgctga
      661 acgtgaaggc cacggctcgg tcgtatccca gagaagccca gctataaagc ccgaccttga
      721 tcagcccgga cttcgagcca ccacccggaa cattcccgag ctgcgcgcag cccgaaaacc
      781 agagtgctgc ccactcggag gagggacaca cgttcacgtt cgaggccggc actccgttcg
      841 tgctctctat ctgcacccgg aaatattccc agtcgccgcg atgaatcacc tggtatttcg
      901 gggtcacggc aatccttcca cgaaatcctt ccgattgaac gctggaaaac gtagccgtca
      961 cattccgcga ctcactcata gttacctggc aggcacgaat gcccgagcaa ggaccagacc
     1021 aaccctgaaa cgtggaaccc ggcttggccg ccgcgatcaa cgtaaccatg gtgcccggcc
     1081 cgtaggactc ttcgcaatcc gagccgcaat tgatcccaac cggattcgag gcgaccgtgc
     1141 ccgcgccggt acccgccttc gtcaccgtca gcttgtaaga agtcggaaca actggattct
     1201 tcctgaacgt cgcgatcgcg accctggcct ggttcatcgt cacctgacag gttgaaccgg
     1261 agcccgcgca ggcgccagac caaccggtaa atgccgaacc cggaccggcg ctcgtggtca
     1321 gagtcaccgc agcgcccgga ctgaaagtac ccgaacaggt ggagccgcaa ttgatgccgg
     1381 ctggaatgct ggagatggtg ccggtacctt cacccttctt taccaccgcc agagtttggc
     1441 gctcggcgga agcgattccc gtcacgggag caggggtcgt ccggtcagct gtggtcccgt
     1501 cgccgagaac tccgaaggcg cccttccccc agcaggccaa ctggccc
//
LOCUS       DAOQFI010000579         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_56689_length_1544_cov_3.281397, whole genome shotgun sequence.
ACCESSION   DAOQFI010000579 DAOQFI010000000
VERSION     DAOQFI010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56689_length_1544_cov_3.281397"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..681)
                     /locus_tag="PLE93_09455"
     CDS             complement(133..681)
                     /locus_tag="PLE93_09455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNH87317.1"
                     /translation="MPEGSRTALLVIDMQNDFVEAGAPRQVSGATEIVPQVASAAERM
                     RMQGDLIVWVCRAYSDDGSNVEAARKPQFLKNPYAVAGTRGAALVDGLAPWPDDLSVV
                     TPSYSAFFRTDLDLKLREAGVSKLVGCGVDLSRCVRSTLVEGISLGYECSVLAPAVAS
                     RDDQALNSNLADLVDLDITVEE"
     gene            complement(871..>1544)
                     /locus_tag="PLE93_09460"
     CDS             complement(871..>1544)
                     /locus_tag="PLE93_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87318.1"
                     /translation="LVSVLMVSEGVDIPRLRVGVYATAARTELFFRQVVGRFIRTTPS
                     PRRQMSYLLLPADSRLKQLAFEIEKERRHAIELGPELADELEELDHELPERSEPGEAF
                     QALSSSGAELDDAILAQTTMQLFATDTFDSGPKPEKTLAPAPAAKKPLKTLAPRSDSA
                     FAVRERLREERKELVADVSRKTGESYQQINSRINRELGVASVSKATRQELEQGNALLE
                     RDLKI"
BASE COUNT          260 a          522 c          508 g          254 t
ORIGIN      
        1 ggcctctacc tctgcggggc cggcacccac cccggcggcg gcgtcaccgc cgcttccggc
       61 cacaacgcgg cccagcggat cctcaaggac cgccgcaggg tcaggtggcc ctggcgccgg
      121 gccgctgcct gactactcct cgaccgtgat gtcgaggtcg accaggtcgg ccaggttcga
      181 gttgagcgcc tggtcatccc ggctggcgac cgccggggcg agcaccgagc actcgtagcc
      241 gagagagatc ccctcgacca gggtcgaccg cacgcagcgg gaaaggtcga ccccgcagcc
      301 gaccagcttg gaaaccccgg cctcgcgcag cttcaggtcg aggtcggtgc ggaagaaggc
      361 cgagtaggaa ggagtgacga cgctgagatc atcgggccag ggggcgagcc cgtcgaccag
      421 tgccgcgccg cgggttcccg caaccgcgta cgggttcttc aggaactggg gcttgcgggc
      481 cgcctccacg ttcgaaccgt catccgagta ggcgcggcag acccagacga tcaggtcgcc
      541 ctgcatcctc atccgctccg ccgccgaggc gacctgcggc acgatctcgg tcgcgcccga
      601 gacctggcgg ggtgctccgg cctcgacgaa gtcgttctgc atgtcgatca ccagcagggc
      661 cgtcctggac ccttccggca tcggtgccgc agccgaaacc tcaagtggcc ccggaaggct
      721 ctgggcggca tacatgactt gcgaacttcc tctccctaat cgaacatcat tgaacagcgc
      781 gatcggagat tacccaaaaa atcctgcccc gaaacgcaga acgccaagtt tcttctagta
      841 cgcgaatggc tttggaaagc cgaaacctgg tcagatcttc agatcccgct ccagcagcgc
      901 atttccctgc tccagctcct gacgggttgc cttgctgacc gaggcaaccc cgagctcgcg
      961 gtttatgcgc gaattgatct gctggtagga ctccccggtc ttgcgggaca cgtcggcgac
     1021 cagttccttg cgttcctcac gcagccgctc ccgcaccgca aaggccgaat cggacctcgg
     1081 ggccagggtc ttcaacggct tcttcgccgc aggagccggg gccagcgtct tctcgggttt
     1141 gggaccggag tcgaaggtat ctgtggcaaa cagctgcatc gtggtctggg cgaggatcgc
     1201 gtcatcgagc tcggcgccgc ttgagctgag agcctggaag gcttctcccg gctcggagcg
     1261 ttcgggcagt tcgtggtcga gttcctccag ttcgtcggcc agctccggtc cgagctcgat
     1321 cgcgtggcgg cgctccttct cgatctcgaa ggcgagctgc ttgagccggc tgtcggcggg
     1381 caggagcaga tagctcatct gcctccgggg cgagggggtg gtgcggatga agcggccgac
     1441 gacctggcgg aagaagagct cggtccgggc ggccgtcgcg tagacaccga cccggagccg
     1501 cgggatgtcg accccctcgg agaccatcag caccgagacc agcc
//
LOCUS       DAOQFI010000580         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_57053_length_1539_cov_3.507412, whole genome shotgun sequence.
ACCESSION   DAOQFI010000580 DAOQFI010000000
VERSION     DAOQFI010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57053_length_1539_cov_3.507412"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLE93_09465"
     CDS             <1..530
                     /locus_tag="PLE93_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87319.1"
                     /translation="PPGPADGKSGREVRAIAASAGKTGARKPARKPATGKRGKPAPSD
                     SPAIRICLAVLGLSALSIALPASFAPETFYTDYPFYTALVKLLPPYNEHLITDVGGLY
                     LGFGVMFIWATIKPSRELVLPLCWGWIAAQILHFAFHVSHLTGFTTTQAVSQQIGLAL
                     YLLVAIVPIALLRRQ"
     gene            complement(728..>1539)
                     /locus_tag="PLE93_09470"
     CDS             complement(728..>1539)
                     /locus_tag="PLE93_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH87320.1"
                     /translation="EVLDDERIAAVTFTGSTGVGISVAERMASRAAPAQAEMGGQNAA
                     IVLEDADLDAAADAIISGAMGFAGQKCTATRRVVVVSQVREQLEAKLVERVRGLSVGD
                     PEQSGVTVGPLISESAVEEFEAARSAALEAGATELAVAEAPEGEGYFVSPALLRQDDP
                     AATVNQEETFGPLLTLIEVADEDAAVEAANATRFGLVGAVHGRDLGRAGRVAGRMTTG
                     LRRVNAPTPGVDFYAPFGGEGQSSFGPREQGRAAREFFTSSSTTTILPSAT"
BASE COUNT          243 a          536 c          500 g          260 t
ORIGIN      
        1 taccgccggg tcctgccgat ggaaaatcag gtcgtgaggt tcgggcgata gcagcgtcgg
       61 ccgggaagac cggcgcgaga aagccggccc gcaagccggc gaccgggaaa cggggcaagc
      121 cggcgccgtc cgactcgccg gcgatccgga tctgcctcgc ggtgctgggg ctctcggctc
      181 tttcgatcgc cctgcccgcc tcgtttgccc cggagacctt ctacaccgac tatccgttct
      241 acacggcgct ggtcaaactg ctgccgccct acaacgagca tctgatcacc gatgtcggtg
      301 gtctctacct gggcttcggg gtgatgttca tctgggccac gatcaaacct tcgcgcgaac
      361 tcgtcctgcc gctctgctgg ggctggatcg ccgcccagat cctgcacttc gccttccacg
      421 tcagccacct gaccggcttc accaccaccc aggcagtaag ccagcaaatc ggcctcgccc
      481 tctacctgct cgtagcgatc gtgcccattg cccttttgcg caggcaatag ggcaatcggg
      541 acgatcttcg gcttgatgta agtccgattg ccctgctgcg cgagcggtag cgcgagcgtg
      601 tgggggatcg agatcggagt tgggtgcgct attcgcggat aacttcaacc ttgtgaaacg
      661 ggagaagtgc gagattcact tggtcggcga ataacgccga cgaagtgcat ctcgatgcat
      721 cccccggtca ggttgcggag ggcaggatcg tcgtggtcga gctggaggtg aagaactcgc
      781 gggcggcgcg accctgttcc ctcgggccga aggaggactg gccctcgcct ccgaagggag
      841 cgtagaaatc aacccccggg gtcggcgcgt tgacccggcg gaggccggtg gtcatccgcc
      901 cggcgacccg gccagcccgg cccagatccc ggccgtggac cgcgccgacc agcccgaagc
      961 gggtggcgtt ggcagcttcg accgccgcgt cctcgtcggc cacttcgatc agggtgagca
     1021 gcgggccgaa agtttcctcc tggttgaccg tcgccgccgg gtcgtcctgg cgaagcaggg
     1081 ccggcgaaac gaaatagccc tcgccctccg gcgcctcggc gacggcaagc tccgtggcac
     1141 ccgcctcgag cgccgccgaa cgggcagcct cgaactcctc gaccgccgat tcgctgatca
     1201 ggggcccaac cgtgactccg ctctgttccg ggtccccgac cgagagcccc cggacccttt
     1261 cgaccagctt tgcttccagt tgctcccgga cctgactgac cacgaccacg cggcgggtcg
     1321 cggtgcattt ctgcccggcg aaacccatcg cgccggagat gatcgcgtcg gctgccgcgt
     1381 caaggtccgc gtcctcgagg acgatggccg cgttctggcc acccatctcg gcctgggcgg
     1441 gggccgcccg ggaggccatc cgctcggcga ccgagatccc caccccggtc gagccggtga
     1501 aggtgacggc ggcaatccgt tcgtcatcga gcacctcgg
//
LOCUS       DAOQFI010000581         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_57142_length_1538_cov_3.017532, whole genome shotgun sequence.
ACCESSION   DAOQFI010000581 DAOQFI010000000
VERSION     DAOQFI010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57142_length_1538_cov_3.017532"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLE93_09475"
     CDS             complement(<1..235)
                     /locus_tag="PLE93_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87321.1"
                     /translation="MNDLATTLCFPALGGEVLIQAEGPGSDLAVEEARELVLDLHRRL
                     TRFEPSSELCQLNRSSDRAVKASPLLLRFADEVH"
     gene            complement(232..594)
                     /locus_tag="PLE93_09480"
     CDS             complement(232..594)
                     /locus_tag="PLE93_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87322.1"
                     /translation="MNELTPKEMAEAKKLARRVRVGNIRRRIAYGATLMVAVFSGAIL
                     FRSLEQQTGTAATNVSAVTRSDESDDSEGLSALVTQVSSAFGDDGEEEEGSPSTTAGT
                     VTGSTTTSSAPPLVTSQS"
     gene            complement(609..1016)
                     /locus_tag="PLE93_09485"
     CDS             complement(609..1016)
                     /locus_tag="PLE93_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87323.1"
                     /translation="MSETHDDQKPDQNDPATTELTPEKGWSATTRRLADNPVGRWVAV
                     GAAVVVALFSGMLIGRATGQDPAEAGPASFGRPDGDGPPAGGQVPFHGDDDFGGQSYG
                     DDDSFEPPEGGDDERSESSSGGSSPSPLETGQS"
     gene            1119..>1538
                     /locus_tag="PLE93_09490"
     CDS             1119..>1538
                     /locus_tag="PLE93_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH87324.1"
                     /translation="MRILLVEDEKAIADFIARGLEAEGHAVTWVADGIDGEALARTGR
                     FDLILLDLMLPGKDGLEVLASLRREDGSTPVILLTAKGEVADRVAGLDAGANDYVTKP
                     FAFEELVARIRAQLRSPAQGSATELRTGDIELDLLRRR"
BASE COUNT          239 a          495 c          526 g          278 t
ORIGIN      
        1 ggtggacttc gtctgcgaat cggaggagga gtgggcttgc cttcacggcc cggtcgctgc
       61 tgcggttgag ctggcagagc tcgctggatg gttcgaagcg ggtcagccgg cggtgcagat
      121 ccaggaccag ctcccgggcc tcttcgaccg cgaggtcgga acccgggcct tccgcctgga
      181 tcaggacttc gccgccgagg gcggggaaac agagggttgt ggccaggtca ttcatgactg
      241 gctggtgacc agcgggggag cgcttgaagt ggtcgtggaa ccggtcaccg tgcctgccgt
      301 ggtcgaaggt gaaccttcct cttcttctcc gtcatccccg aatgccgatg aaacctgcgt
      361 gacgagcgcg ctcaggccct cggagtcatc gctctcgtcg ctgcgggtca cggccgaaac
      421 gttggtcgcg gcggttcccg tctgctgttc cagcgatcgg aagaggatgg cgccgctgaa
      481 caccgcgacc atcagcgtcg ctccgtaggc gatccggcgg cggatgttcc cgacccgaac
      541 ccggcgggcc agcttcttcg cctcggccat ttcttttggg gtcagttcat tcatgctcat
      601 gtacctcctc aggactgtcc ggtctccagc ggcgagggcg aggagccgcc ggaggaactt
      661 tcactgcgtt cgtcatcgcc cccctctggc ggttcgaagc tgtcgtcgtc gccatagctc
      721 tggccgccga agtcgtcgtc gccgtggaag ggcacctggc caccagccgg aggtccgtcg
      781 ccgtccggcc ggccgaaaga tgcgggtccg gcctcggccg ggtcctgccc ggtggcccgc
      841 ccgatcaaca ttccgctgaa gagcgcaacg acgacggcgg cacccaccgc gacccagcgg
      901 cccaccgggt tgtcggcgag cctgcgggtt gtcgccgacc accccttttc cggggtcagt
      961 tccgtggttg cgggatcgtt ctgatcgggc ttctggtcgt catgcgtttc gctcatgtcc
     1021 ggaaggttga tccagccggc ttggaggatc cgtaacgaaa catgagagtt ctcttagaaa
     1081 cgcgatcccg gccggattcg cagttacgat cggcaccgat gcgcatcctc ctggtcgaag
     1141 acgaaaaggc gatagccgac ttcatcgccc gtggccttga agcggagggt catgcggtga
     1201 cctgggtcgc ggacgggatc gacggggaag ccctcgccag aaccggacgc ttcgacctga
     1261 tcctgctcga cctgatgttg cccggcaagg acggcctcga ggtgctcgct tcgctgcgcc
     1321 gggaggacgg gtccacgccg gtgatcctgc tcaccgcgaa gggggaggtc gccgaccggg
     1381 tggccggact cgacgccggc gcgaacgact acgtcacgaa gccattcgcc ttcgaggaac
     1441 tggtcgcgag aatccgggcc cagctgagaa gcccggccca gggcagcgcg accgagctca
     1501 ggaccggtga catcgaactg gatctgctgc gccggcgg
//
LOCUS       DAOQFI010000582         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_57165_length_1538_cov_2.358058, whole genome shotgun sequence.
ACCESSION   DAOQFI010000582 DAOQFI010000000
VERSION     DAOQFI010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57165_length_1538_cov_2.358058"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLE93_09495"
     CDS             <1..244
                     /locus_tag="PLE93_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNH87325.1"
                     /translation="YAIVASKGGADEPPAWYLNLQADPTVEVQVKADKFTARARTASE
                     EEKAGIWPQMVKQWPAYDDYQAATDRPIPIVLLERA"
     gene            complement(248..>1538)
                     /locus_tag="PLE93_09500"
     CDS             complement(248..>1538)
                     /locus_tag="PLE93_09500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH87326.1"
                     /translation="NVTSTPEQVQRVYLTLMLGNTLAASFIWGINTIFLLDAGLSNLE
                     AFAANAFFTAGMVIFEIPTGVVADTIGRRVSYMAGTLTLMATTLLYVMLWEINGPFWA
                     WAVISLLLGLGFTFFSGAVDAWLVDALDETGFTGDLERVFGRGMTVTGIGMLAGSVIG
                     GYVAQVTSLGVPFVMRAVVLLAMFFFAAWAMKDLGFKPHKAESTFGEMKSITRNSIDY
                     GWRVPSVKWIMLAAPFTAGVGIYAFYALQPYLLELVGDPHAYGIAGITAALVAGSQII
                     GGFLAPRIRRRFRLRTSALLLAVSVGAVTLALIGVIEQFWVAVGLIATWAMLSSAATP
                     IRQSYINGMIPSRQRATILSFDSMLGSTGGVVVQPALGRVADTSGYATSYLYGAMITV
                     FALPFILLSRHQHPPADRATGTPEAEGTVEAGPESDR"
BASE COUNT          317 a          490 c          517 g          214 t
ORIGIN      
        1 ctacgcgatc gtcgcctcga agggcggggc ggacgaaccg ccggcctggt acctgaacct
       61 gcaggccgac ccgacggtcg aggtccaggt gaaggcggac aagttcacgg cccgggcgag
      121 gaccgcgagc gaggaagaaa aggccgggat ctggccgcag atggtcaaac agtggccggc
      181 ctacgacgat taccaggcgg caaccgaccg gccgatcccg atcgtcctgc tcgaaagggc
      241 ctgaacctca ccgatcagat tcgggcccgg cctcgaccgt gccttccgcc tccggagttc
      301 cggtcgcgcg gtcggccggc gggtgctggt gccgggacag gaggatgaac ggaagggcaa
      361 agacggtgat catcgcgccg tagaggtagg aggtcgcgta cccggaggtg tcggcgaccc
      421 ggccgagcgc gggttggacc acgaccccgc cggtcgatcc cagcatcgag tcgaaggaaa
      481 ggatcgtcgc ccgctggcgg gaggggatca tcccgttgat gtaggactgc cggatcgggg
      541 tcgccgccga cgagagcatg gcccaggtgg cgatcagtcc gaccgcgacc cagaactgct
      601 cgatcacccc gatcagggcg agcgtcaccg caccgacaga gaccgccagg agcagcgcgg
      661 atgtccgcaa cctgaaacgg cggcggatcc tcggggcgag gaagccgccg atgatctggg
      721 agcccgctac cagcgcagcg gtgatgccgg cgatgccgta ggcatgcggg tctccgacca
      781 gctccagcag gtatggctgc agcgcgtaga aggcataaat cccgaccccg gcggtgaagg
      841 gagccgcaag catgatccac ttgaccgaag ggacccgcca gccgtaatcg atcgaattcc
      901 gggtgatcga cttcatttcg ccgaaggtgc tttcggcctt gtgcggcttg aaaccgaggt
      961 ccttcatcgc ccatgcggca aaaaagaaca tcgccagcag aacgaccgct ctcatcacga
     1021 acggcacccc cagactggtc acctgggcca cgtagccgcc gatcaccgag ccggccagca
     1081 tcccgattcc ggtcacggtc atgccccggc caaagacccg ttccaggtct cccgtgaacc
     1141 ccgtttcgtc cagtgcgtca accagccagg catcgacggc gccggagaag aaagtgaaac
     1201 cgagcccgag cagcagtgat atgaccgccc aggcccagaa gggtccgttg atctcccaga
     1261 gcatcacgta gagcagcgtg gtggccatca gggtcagggt cccggccatg taggagaccc
     1321 ggcggccgat cgtgtcggcg acgaccccgg tcggaatctc gaagatcacc atgccggccg
     1381 tgaagaaggc gttggcggcg aaggcctcca ggttggaaag cccggcatcc agaaggaaga
     1441 tcgtgtttat cccccagatg aacgaggcag caagcgtgtt gcccagcatc agggtcaggt
     1501 agacccgctg aacctgctcc ggcgtggagg tcacgttg
//
LOCUS       DAOQFI010000583         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_57886_length_1529_cov_2.162822, whole genome shotgun sequence.
ACCESSION   DAOQFI010000583 DAOQFI010000000
VERSION     DAOQFI010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57886_length_1529_cov_2.162822"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLE93_09505"
     CDS             <1..348
                     /locus_tag="PLE93_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87327.1"
                     /translation="AWMCFATLLPLGIMQLYKSVDDGYFEARQLDFLTDKTTEMLEWM
                     RLPGDVVFIVFGAIPVLYMCFLGVKYTIARRGEDGDGRGEADAELFTVISGTSMEDEP
                     PEISGRPEPTPGT"
     gene            345..896
                     /locus_tag="PLE93_09510"
     CDS             345..896
                     /locus_tag="PLE93_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87328.1"
                     /translation="MNTEVLLAVGYAALLLGGALLLEWLSRHTHNRSQAWRTSGFDYD
                     AQHDFWVCHKGEQLWPTEFDREKRLVRYRAKATVCNACPAKVDCTDSHHGREVTRAVD
                     SWPHSEAGRFHRAIAVMLVVLAVFELAVVLVRNHEPSQIAVLLPLFLLALAVTWWLAV
                     DLIRTPSNFPEAKPAHGNRIGAK"
     gene            893..1330
                     /locus_tag="PLE93_09515"
     CDS             893..1330
                     /locus_tag="PLE93_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNH87329.1"
                     /translation="MKRSEALQPLSREHHHALYLAKVIRDNGGGGDSPGRFLSFWREE
                     GAIHFRIEEEVLLPGSGLAGPGQDEEVARMLDDHLEIRRAAARIEAGEAGPDELVEVA
                     ERLTAHVRFEERELFPRVERELSSEQLRELATAIEAAEQAAET"
     gene            complement(1350..>1529)
                     /locus_tag="PLE93_09520"
     CDS             complement(1350..>1529)
                     /locus_tag="PLE93_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87330.1"
                     /translation="TALPTQSECLKVLGCDGPGKVNKLAEIIAATVLCGDLSLGSAVV
                     ADEWVEAHDELGRNR"
BASE COUNT          254 a          486 c          514 g          275 t
ORIGIN      
        1 gcctggatgt gctttgcgac cctgctgccg ctggggatca tgcagctcta caagtcggtg
       61 gacgacggct atttcgaggc ccgccagctc gacttcctga ccgacaagac gaccgagatg
      121 ctcgagtgga tgcggctgcc cggtgacgtg gtattcatcg tcttcggtgc cattcccgtg
      181 ctctacatgt gtttcctcgg agtcaagtac acgattgccc gtcgcggcga agacggggac
      241 ggccggggtg aagcggacgc cgaattgttc acggtgatct ccggcacttc catggaagac
      301 gaaccgccgg agatctccgg ccggccggaa ccgaccccgg gaacgtgaac accgaggtcc
      361 tcctggcagt cggatacgcg gcccttctgc tgggaggggc tctgcttctc gagtggcttt
      421 cccgccacac tcacaaccgg tcgcaagcct ggcggacctc cggcttcgat tacgacgccc
      481 agcatgactt ctgggtctgc cacaaagggg aacagctctg gccgaccgag ttcgaccggg
      541 aaaagcggct ggtcaggtac cgggccaagg cgacggtgtg caatgcctgc ccggccaaag
      601 tggactgcac cgattcccat cacggccgcg aggtcacccg tgcggttgat tcctggccgc
      661 attcggaagc cggccgattt catcgggcga tcgcggtgat gctggttgtc ctggctgtgt
      721 tcgaactggc cgttgtgctg gtccgcaacc acgagccgtc acagatcgcg gtgctcttgc
      781 cgctgttcct gctcgccctc gcggtgacct ggtggctggc cgttgacctg attcgcaccc
      841 cgtcgaactt tcccgaggcg aagcccgccc acggaaatcg catcggtgcg aaatgaagcg
      901 gtccgaggca cttcagcccc tgtcacgcga gcatcaccac gcgctctacc tggccaaagt
      961 gatccgcgac aacgggggcg gaggggattc accggggcgt ttcctcagtt tctggaggga
     1021 ggaaggggcg atccatttcc ggatcgaaga ggaagtgctc ctgccggggt ccgggctcgc
     1081 cgggcccggg caggacgagg aagtcgcccg catgctcgac gatcaccttg agattcgccg
     1141 ggcggcggcc cggatcgagg ccggcgaggc cgggccggac gagttggtcg aagtcgccga
     1201 gaggctcacc gctcacgtcc gtttcgagga gcgggaactg ttcccgcggg tcgaacggga
     1261 gctctcgagc gagcagcttc gggaactcgc gaccgctatc gaggcggccg agcaggcggc
     1321 ggaaacctga gtcgccgggg gcgcttgccc tagcggttgc ggccgagttc gtcgtgggct
     1381 tcgacccact cgtcggcgac caccgccgag ccgagtgaaa ggtctccgca aagcacggtc
     1441 gcggcaatga tctcggccag cttgttgacc ttgcccgggc cgtcgcaacc cagaaccttc
     1501 aggcattcgc tctgggtcgg cagcgctgt
//
LOCUS       DAOQFI010000584         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_57940_length_1528_cov_2.920570, whole genome shotgun sequence.
ACCESSION   DAOQFI010000584 DAOQFI010000000
VERSION     DAOQFI010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57940_length_1528_cov_2.920570"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..903)
                     /locus_tag="PLE93_09525"
     CDS             complement(151..903)
                     /locus_tag="PLE93_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNH87331.1"
                     /translation="MQDRGLAAYIAELLGTFFLVFGITLVGVLFIAVGDNASFGSDFA
                     VVGLTYAFILTALIFAFGGPSGGHFNPAITLGAALMKKIAPVDAVIYILAQLSGGVLG
                     ALLTKGLLEDEGRAANYGAVSISPLLSGNFAGGVVECLGTFLLVLAVLSVALGVRAVK
                     DWAPLAIGLTLGVVGLILVPLTGGAVNPARWFGPALVSGEWGGVWPYLGGEIVGAVLA
                     FVVYKFVIAPGVDDGSRSEPAKKPSTIPSPEA"
     gene            complement(1085..1405)
                     /locus_tag="PLE93_09530"
     CDS             complement(1085..1405)
                     /locus_tag="PLE93_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87332.1"
                     /translation="MARVLALVPDLMLSSRVSGMLGAAGHEVTVAPTPPVAADFDFDL
                     IVCDLEACDPAAVAKLPAPAIGFYSHVDVETRDRGREAGLDLVVPRSRMARELPGLAA
                     RLLD"
BASE COUNT          298 a          502 c          517 g          211 t
ORIGIN      
        1 gttgcgatca gcctggccct cggcctcgcc gcggtcacgc tcggccaggc gatcacctga
       61 gccgggccgc ccccgacgac gaagggcggc ccggaggccg ccctttcgaa actgctcgag
      121 ttttgagccc ggcgtgccgg gcttgccgct ctaggcctcc ggcgacggga tcgtcgaggg
      181 cttcttggcc ggttcggacc gggatccgtc gtcgacgcca ggcgcgatca cgaacttgta
      241 gaccacgaag gcgaggacag caccgacgat ttcaccgccg aggtagggcc agacaccgcc
      301 ccattcgccc gaaacgaggg cggggccgaa ccagcgggcc gggttgacgg cgccaccggt
      361 cagcggaacc aggatcaggc cgacgacacc gagcgtgagt ccgatcgcca gcggagccca
      421 gtccttgacc gcacggacgc cgagggcaac cgaaagcaca gccagaacca gcaggaaggt
      481 accgaggcac tcgaccacgc cgccggcgaa gttgccgctc agcagcgggc tgatcgacac
      541 cgcaccgtag ttggcggcgc gcccttcgtc ttccagcaga cccttggtca gcagagcgcc
      601 gaggacacca ccggagagct gggcgaggat gtagatcact gcgtcaaccg gggcgatctt
      661 cttcatcagc gccgcaccga gcgtgatcgc cgggttgaag tgaccaccgc tgggcccgcc
      721 gaaggcgaat atcagcgccg tgaggatgaa ggcgtaggtc agaccgacga ccgcgaagtc
      781 agaaccgaat gaggcgttat cgcccacggc aatgaaaagc acgccgacca gagtgattcc
      841 gaacaccagg aagaacgttc ccagcagttc ggcgatatat gcggcgagac cgcgatcttg
      901 catgaagtca aacctcccgt ggcttgtatc gattgcgccc caccgtttgc ggcgagccgt
      961 ggcagtctaa ccagagcccg ttgccgaacg gttggaagcg caatacgggg tcgcatgttt
     1021 gggcctagaa atatacgccg acaaaggccc ggttcctgcc gcgggagggt cggggtgagg
     1081 gttttcagtc cagcagccgg gccgcgaggc cgggcagttc ccgggccatg cgggaccggg
     1141 gaacgaccag gtcgagaccg gcttcccggc cccggtcgcg ggtctcgacg tcgacatgcg
     1201 agtagaaacc gatcgcgggc gcaggcagct ttgccaccgc cgcggggtca caggcttcga
     1261 gatcacagac gatcaggtcg aagtcgaaat cggcggccac cggaggggtt ggcgcaaccg
     1321 tcacctcatg gccggcggca ccgagcatcc cgctgacccg gctcgaaagc atcaggtcgg
     1381 gaacaagggc gagcacgcgg gccattgcgg ttgccggacc gggcctcaga tcgagcggcg
     1441 ctgacggaac tcttcgcgca cgctctccgg ccggccccac atctgaacta cctcggagcg
     1501 gccttcacgg acctcgcgca ggttgagc
//
LOCUS       DAOQFI010000585         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_58093_length_1526_cov_2.651258, whole genome shotgun sequence.
ACCESSION   DAOQFI010000585 DAOQFI010000000
VERSION     DAOQFI010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58093_length_1526_cov_2.651258"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..756
                     /locus_tag="PLE93_09535"
     CDS             130..756
                     /locus_tag="PLE93_09535"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNH87333.1"
                     /translation="MTSDRARRELDSRELPAGQFARLLKAVVVPRPIAWVSTTSAEGV
                     DNLAPHSFFTFASEVPPVLQFTSIGHKDSLRNATGTGEFVINLADLGNLDAVNASGTA
                     YPPDEDEFAALGIEREPSVAVRPPRVAGSPVAIECRTEDTVEFDESVVVFGRVVHLAI
                     SEAVLDGDHPDIGKLAPMARLGRNEWSEIGKVHRIDRIPLDRARRIGS"
     gene            complement(819..>1526)
                     /locus_tag="PLE93_09540"
     CDS             complement(819..>1526)
                     /locus_tag="PLE93_09540"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNH87334.1"
                     /translation="ALFNALKIIDKPIEELRVLMVGLGAAGVAVTKMMLAAGLTHVVG
                     CDRKGALSVTREDYQAGELSGIKKWFADNSNPDHLLGTPDEVIEGMDVFVGLSGPGII
                     QPESLGKMNRDPIVFAMSNPDPEVAPEEAAKYVRIMATGRSDYPNQINNVLCFPGIFR
                     GALDAGAPQITEEMKMAAARGIADVVGDDLSEDYIIPSVFDRDVAPRVAEAVIQEAKR
                     AGDARFNVETGAFASLG"
BASE COUNT          245 a          507 c          510 g          264 t
ORIGIN      
        1 cgacacggtg accaccggcc ccgccgctca ccgcaacaag gtgatccgcg accgccggga
       61 caaggtcgtc tggggctggg gcctggccgc tttctagccg ttccggctgc agtccggcag
      121 aatccgggca tgacctccga ccgggccaga agggaactcg attccaggga attgccggca
      181 gggcagttcg cccggctcct gaaggcggtt gtggtgccgc gcccgatcgc ctgggtctcg
      241 accacttccg ccgagggcgt tgacaacctg gccccgcatt cgttcttcac tttcgcctcg
      301 gaggttcctc cggttctcca gttcacctcg atcggccaca aggactcact tcgcaacgcc
      361 accgggacgg gtgagttcgt gatcaacctg gccgacctcg gcaacctcga cgcggtcaac
      421 gcatccggga ccgcctaccc gccggatgag gacgagttcg cggcgctcgg gatcgaacgg
      481 gagccctcgg tcgcggtcag gccgccccgg gtggccggtt caccggtggc gatcgaatgc
      541 cggaccgaag acaccgtcga gttcgacgaa tcggtggtcg tcttcggccg ggtggttcat
      601 ctggcaatca gcgaagcggt gctggacgga gatcatcccg acatcgggaa gctcgccccg
      661 atggcccgtc tcggccgcaa cgagtggtcg gagatcggca aggtccaccg gatcgaccgg
      721 atccccctgg atcgggcgcg ccgaatcggg agctagaccg tcgaatgccc actttgtgcc
      781 ggtatgtggc gcaaaatggg ttctcgccgc agggagactc agcccaggga ggcgaaggcg
      841 cccgtctcga cgttgaagcg cgcgtcgccg gcccgcttgg cttcctggat caccgcctcg
      901 gctacccggg gtgcgacgtc acggtcgaag accgagggga tgatgtagtc ctcggagagg
      961 tcgtccccga ccacgtcggc gatgccccgg gcggcggcca tcttcatctc ctcggtgatc
     1021 tgtggcgcgc cggcgtcgag tgcgccgcgg aagattcccg ggaaacagag cacgttgttg
     1081 atctggttcg ggtagtcgga gcggccggtg gccatgatcc gcacgtattt ggcggcttcc
     1141 tcgggggcaa cctccgggtc cggattcgac atggcgaaga cgatcggatc ccgattcatc
     1201 ttgcccagcg actcgggctg gatgatgccc gggcctgaca ggccgacgaa cacgtccatg
     1261 ccttcgatca cctcgtccgg agtgccgagc agatggtccg ggttcgagtt gtcggcgaac
     1321 cacttcttga tcccgctcag ctcgccggcc tggtaatcct cgcgagtgac cgaaagggcg
     1381 cccttgcggt cgcagccgac cacgtgggtg aggccggcgg caagcatcat cttggtcacc
     1441 gccacgccgg cggcgccgag gccgaccatc agcacccgca gctcctcgat cggcttgtcg
     1501 atgatcttca gcgcgttgaa cagcgc
//
LOCUS       DAOQFI010000586         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_58151_length_1525_cov_3.308844, whole genome shotgun sequence.
ACCESSION   DAOQFI010000586 DAOQFI010000000
VERSION     DAOQFI010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58151_length_1525_cov_3.308844"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="PLE93_09545"
     CDS             complement(<1..1281)
                     /locus_tag="PLE93_09545"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNH87335.1"
                     /translation="MSPRNYSEDELVEGPTLELLESIGYEIVDGYSEPAEFGGLGRDS
                     QTEVVLKHRLAPKLEELNPELPESAIDEAVEELVRDRATMDPVRANREVYKKLRGGVK
                     VTVVNDEGDRVEEKVRLIDWNDPANNDYLAISQFWIQGPLHKRRCDIVCFVNGIPLIL
                     MELKASHKSVHEAYDKNLRDYRDTIPKLFTQNAFVVLSTGSETRVGSSFAPWERFGEW
                     KRVDDESELGVVSLETAVDGLCSPAKLLDTVENFIAYLERPGGLIKVLAQNHQVLGVN
                     AAIRALRDRETRDGRLGVFWHTQGSGKSLSMLFFTQKVLRKEPGNWTFVMVTDRSELD
                     EQLYGEFKDAGVVEGHLQATSSTNLRQLLTEDHRYVFTLIHKFRPPEGEEMPVCSDRS
                     DVIVITDEAHRSQYSTLAMNMRKALPNADFLGFTG"
     gene            complement(1278..>1525)
                     /locus_tag="PLE93_09550"
     CDS             complement(1278..>1525)
                     /locus_tag="PLE93_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87336.1"
                     /translation="GATFKEITKGAFKKFPILVPESSVLEAFSGHADPIGEQIADFES
                     HNEALKETRDLLLPRLVTGKLDISDIDLGVLEPKEAA"
BASE COUNT          285 a          469 c          440 g          331 t
ORIGIN      
        1 accggtgaag ccgagaaagt cggcgttggg cagcgccttc ctcatgttca tcgccagggt
       61 cgagtactgg ctccggtgtg cctcgtccgt aatcacgatc acgtccgagc ggtcggagca
      121 gaccggcatc tcttcgccct cgggcgggcg gaacttgtgg atcagggtga acacataccg
      181 gtggtcctcg gtcaagagct gccgcagatt cgtggacgag gtggcctgga ggtgcccttc
      241 gaccacccca gcgtccttga actctccgta gagctgttcg tcgagttcgc ttcggtcggt
      301 gaccatgacg aaggtccagt taccaggctc cttgcgcagg accttctggg tgaagaacag
      361 catcgaaagg ctcttgccgg aaccctgggt gtgccagaac actccgagtc ggccgtcccg
      421 ggtctcgcgg tcacgcagtg cccggatcgc ggcgttcacg ccgagcacct gatggttctg
      481 ggcgagcact ttgatcaacc cgcctggccg ttcgaggtag gcaatgaaat tctcgaccgt
      541 atccagcagc ttggccggcg agcaaagtcc gtcgaccgca gtttcgagcg agaccacacc
      601 gagctcggat tcgtcgtcga cccgtttcca ttcgccgaat ctctcccagg gcgcgaagct
      661 cgatccgact cttgtctctg aaccggtcga gagcacgacg aaggcgttct gggtgaacag
      721 cttcgggatg gtgtcccggt agtcccggag gttcttgtcg taggcctcgt gaaccgactt
      781 gtgggaagcc ttcagctcca tcaggatcag cggaattccg ttgacgaagc agacgatgtc
      841 gcaacggcgt ttgtgcagtg gcccctgaat ccagaactga ctgatcgcca ggtagtcgtt
      901 gtttgccgga tcgttccagt cgatcaggcg gactttttcc tcaacccggt caccttcatc
      961 gttcacgacc gtgactttga ccccgccacg cagtttcttg tagacctccc ggttcgcgcg
     1021 caccggatcc atggtcgcgc gatcacgaac cagttcctcg accgcctcgt cgatagccga
     1081 ctccggcagt tcggggttga gttcctccag tttcggagcg agccggtgct tcagcaccac
     1141 ctcggtctgg ctgtcgcgac caagaccacc aaactcggcc ggttcggagt agccatcaac
     1201 gatctcatag ccgatcgact ccagaagctc gagagtcggc ccttcaacca gctcatcctc
     1261 cgaatagttc cgagggctca agcagcctcc ttcggttcga ggactccaag gtctatatcg
     1321 gaaatgtcca gcttgccggt gaccaagcgg ggcagcaata gatccctcgt ttccttcaag
     1381 gcctcattgt gagactcgaa atcagctatc tgttcgccga tcgggtcggc gtgtccggag
     1441 aacgcctcaa gcaccgagct ttcgggaacc aggattggaa acttcttgaa ggcccctttc
     1501 gtgatttctt taaacgttgc tccag
//
LOCUS       DAOQFI010000587         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_58456_length_1521_cov_3.396999, whole genome shotgun sequence.
ACCESSION   DAOQFI010000587 DAOQFI010000000
VERSION     DAOQFI010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58456_length_1521_cov_3.396999"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PLE93_09555"
     CDS             <1..878
                     /locus_tag="PLE93_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87337.1"
                     /translation="FTVMGSPTVIARYSYSSDEPPQVAARLVDISADGTTKTLVNRGV
                     WRLPDTGFQVFQLNPNGWHVDEGHKLRLELLPFDGGNADPVAGLLSNFGRPTNNEPAI
                     TVEDLELRVPVKEQPGSLSGLVKAPAKRVLPNRPGVELAPGNEDIGSETIEQYRERNY
                     PVVGKIKITGATVKGKTLTAKVFCAGSNDSCARAKVSFKGAPKKGKGKGVVLATKSNV
                     TGPAGKTTAVRFNLTAKARALFKDTVKRKVTRKNGRKKVKKVKVSGLKSLRSQVLIGG
                     KSAGFITVKRTGKVN"
     gene            1154..>1521
                     /locus_tag="PLE93_09560"
     CDS             1154..>1521
                     /locus_tag="PLE93_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87338.1"
                     /translation="MRKFAVAFAALFMLVVVPNTFAAPPSSIFNGTIPCTTVGDGRTV
                     CQGPGGDTNATPGVNNAVANTVPSWDGTPIDVTVVLPDASTFGPGPYPLAMYFHGFGG
                     EKEGADGDLKRFTDLGFAAFS"
BASE COUNT          303 a          499 c          477 g          242 t
ORIGIN      
        1 gtttcacggt gatgggctcg ccgaccgtga tcgccaggta cagctacagc agtgacgagc
       61 cgccgcaggt tgcggcgcgc ctggtcgaca tctcggccga cggcaccacc aagacactgg
      121 tcaaccgcgg agtctggcgc ctgcccgaca ccggtttcca ggtcttccag ctgaacccga
      181 atggctggca tgttgacgag ggccacaagc tccgtctcga gttgctgccc ttcgacggcg
      241 gcaacgccga tccggtcgcc ggactgctgt ccaacttcgg gcgtccgacc aacaacgagc
      301 cggcgatcac cgtcgaggat ctcgaactca gggtcccggt caaggaacag cccgggtcgc
      361 tgagcggtct ggtcaaggca cccgccaagc gggtcctgcc gaatcgtccg ggtgttgaac
      421 tcgcccccgg caacgaggac atcggctcgg agaccatcga gcagtaccgc gagcggaact
      481 acccggtggt cggcaagatc aagatcaccg gcgccaccgt caagggcaag accctgaccg
      541 cgaaggtgtt ctgcgccggc tcgaacgact cctgcgcccg ggccaaggtg tccttcaagg
      601 gtgccccgaa gaagggcaag ggcaagggcg tcgtactggc gaccaagagc aacgtcaccg
      661 gcccggccgg caagaccacg gctgtcaggt tcaacctgac cgcaaaggcc cgcgcgctgt
      721 tcaaggacac cgtgaagcgc aaggtgaccc gcaagaacgg ccgcaagaag gtcaagaagg
      781 tcaaggtcag cggactcaag tcgctccgca gccaggtgct gatcggcggc aagtcggccg
      841 gcttcatcac cgtcaagcgg accggcaagg tcaactaaac gagagtctcc ggacgacacc
      901 gggggtacct gaaaagtaac gagcgcttcg cgctctgatt aaggagtcac ttttcaggta
      961 cccccggtac cgtcctgacc caacgagacg cttggggagt ccgtcgacgg cgggctcctt
     1021 ttcgtgttgg tttcgtgaac gggtggtaat ctcaatagcc aggggatgtg ccaaccggcg
     1081 cgtgcccgag agagtcgtcc aagggaagcc gcagggagcg gcaaccgttt caatagggag
     1141 aaacagggat caaatgcgta agttcgccgt agcgttcgct gcacttttca tgctcgtcgt
     1201 cgtgcccaat accttcgccg cgccgccgag ctcgatcttc aatggaacga tcccctgcac
     1261 gaccgttgga gacggtcgaa ccgtctgcca gggtccgggc ggcgacacca acgccacgcc
     1321 cggtgtgaac aatgcggtcg ccaacaccgt ccccagctgg gacggcaccc cgattgacgt
     1381 caccgtcgtc ctgcccgacg ccagcacctt cggccccggt ccctacccgc tggccatgta
     1441 cttccacggt ttcggcggcg agaaggaggg tgccgacggc gacctcaagc ggttcaccga
     1501 tctgggcttc gccgccttct c
//
LOCUS       DAOQFI010000588         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_58812_length_1516_cov_3.520192, whole genome shotgun sequence.
ACCESSION   DAOQFI010000588 DAOQFI010000000
VERSION     DAOQFI010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58812_length_1516_cov_3.520192"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1516)
                     /gene="lon"
                     /locus_tag="PLE93_09565"
     CDS             complement(<1..>1516)
                     /gene="lon"
                     /locus_tag="PLE93_09565"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNH87339.1"
                     /translation="DQLHGVGVVGVVTRMLKVPDGTIRVLVQGSQRVRLGDFVTEKPY
                     LVARITEMPDVIEETPELEALTRNVQKTFSDIIEQIPYLPEELQLAVTNVDDPSALSH
                     LIAGALRITTDEKQLLLEEVDVAKRLRMLSEILARELEVIQLGSQIQSEVQSSIDKGQ
                     REYFLREQLKAIQTELGEEDEQTAEINELRERIEAAKLPEHAHQAAERELSRLEKLPP
                     AAAEHGVIRTYLEWLVDLPWSKESKDNLDIEHAREVLDADHYDMEKIKDRILEYLAVR
                     KLNPDSPGPILCFVGPPGVGKTSLGQSIARALGREFERISVGGVRDEAEIRGHRRTYI
                     GAMPGTIVRALRDAGTRNPVFMIDEIDKMGSDVRGDPSSAMLEVLDPAQNDAFRDHYL
                     DMPFDLSEVMFIATANILDTIPGPLRDRMEVIQLAGYTADEKRHIARRYLVPRQIRAN
                     GLKPSQISFTDPALTAIIEEYTREAGVRNLEREIGTVCRKVARTVAEKGGKAKKV"
BASE COUNT          237 a          479 c          501 g          299 t
ORIGIN      
        1 tgaccttctt cgccttgccg cccttctcgg cgaccgtgcg ggcaaccttc cggcagacgg
       61 tgccgatctc ccgttcgaga ttccgcaccc cggcctcccg ggtgtattcc tcgatgatcg
      121 cggtcagcgc gggatcggtg aaggagatct gcgagggctt cagtccgttg gcccggatct
      181 gtcgcggcac gaggtaacga cgggcgatgt gccgcttctc gtcggcggtg taaccggcca
      241 gctggatcac ttccatgcgg tcccggagcg gccccgggat cgtgtcgagg atgttcgcgg
      301 tggcgatgaa catgacctcc gaaagatcga agggcatgtc caggtagtga tcgcggaaag
      361 cgtcgttctg ggccgggtcg agaacctcga gcatggccga ggaggggtcg ccgcgaacgt
      421 cggagcccat cttgtcgatc tcgtcgatca tgaagacggg gttcctggtt ccggcgtcgc
      481 ggagggcccg cacgattgtg ccgggcatcg ccccgatgta ggtgcggcgg tggccgcgga
      541 tttccgcttc atcgcgaact ccgccaaccg agatgcgctc gaattcacgg cccagcgccc
      601 gggcgatcga ctggccgagg ctggtcttgc caaccccggg cgggccgacg aagcagagga
      661 tcggccccgg cgagtccgga ttcagcttgc ggaccgcgag gtactcgagg atgcggtcct
      721 tgatcttctc catgtcgtag tggtcggcgt caagcacctc gcgggcatgc tcgatgtcga
      781 ggttgtcctt cgattccttc gaccacggca ggtcgaccag ccactcgagg taggtccgga
      841 tcacgccgtg ctcggccgcg gccggcggca gtttctccag ccgggaaagc tcgcgttcgg
      901 cggcctgatg ggcatgctcc ggcagtttcg ccgcctcgat ccgctcacgc agttcgttga
      961 tctcggcggt ctgctcgtcc tcttcgccga gttcggtctg gatcgccttg agttgctcgc
     1021 gcaggaagta ctcgcgctga cccttgtcaa tcgaggactg gacttcggac tgaatctgtg
     1081 aaccgagctg gatgacctcc agctcccggg cgaggatctc cgacagcatc cgcagccgtt
     1141 tggcgacgtc gacttcctcc agcagaagct gcttctcatc ggtcgtgatc ctcagagcac
     1201 cggcgatcag gtgcgaaagc gccgacgggt catcgacatt ggtcaccgcg agctggagct
     1261 cctcgggcag gtaggggatc tgctcgatga tgtcggagaa ggtcttctgg acgttgcggg
     1321 tcaatgcctc gagttcgggg gtctcctcga tgacatcggg catttcggtg atccgggcga
     1381 cgagatacgg cttctcggtc acgaaatcac ccagccgaac ccgctggctg ccctggacca
     1441 ggacccggat cgtcccgtcc ggaaccttga gcatccgggt aacgactccg accaccccga
     1501 ccccgtggag ctggtc
//
LOCUS       DAOQFI010000589         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_58986_length_1514_cov_2.809459, whole genome shotgun sequence.
ACCESSION   DAOQFI010000589 DAOQFI010000000
VERSION     DAOQFI010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58986_length_1514_cov_2.809459"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLE93_09570"
     CDS             complement(<1..474)
                     /locus_tag="PLE93_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87340.1"
                     /translation="MSFAELLRATVFLAAGGATALGAIAVIGVRDAENTTILLIGAVW
                     WIVAAWIGLSLGASDRSADAMRETLANARTVNSNSPGVSMPSPGRVAWGRLSPILGAV
                     GLAGALGVVFPEVAVVGSGYALLVALAWRNREAAVLAIEGRDGVRFLVESASPLKP"
     gene            complement(478..1314)
                     /gene="otsB"
                     /locus_tag="PLE93_09575"
     CDS             complement(478..1314)
                     /gene="otsB"
                     /locus_tag="PLE93_09575"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNH87341.1"
                     /translation="MTGTDSPLSVLLEDPANSALMSDVDGTLCRIVDRPDQARVPVRA
                     RESLETLADHLALVACITGRPSPVARKMVGAKKITYFGNHGLSFIKPGQDRPTVVIEG
                     EEAERARNFIRENDNPFWVTSKIRIEDKGPIQALHWRGAGHRTEEVIRGIADHAQSAG
                     LATHWGRKVLELRPPGMEGKAGAVDELLDGTDIDTVVFAGDDRTDYDAAVRLKKLADE
                     GQLKRLLLVAIDSDEGPPELVELADLVVAEPEEWIDLIEGLAGTFSREPAEAQPGAPQ
                     PE"
BASE COUNT          235 a          518 c          518 g          243 t
ORIGIN      
        1 cggcttgagc ggcgaggccg attcgaccag gaagcgcacg ccgtcccgtc cctcgatcgc
       61 caggactgcg gcctcgcggt ttcgccaggc caaagcgacc agcagggcgt agccggaacc
      121 gaccaccgcc acctcgggga agacgacgcc gagcgcaccg gccagaccga ccgcgccgag
      181 gatcgggctg aggcgccccc aggcgactcg ccccggcgag ggcatcgaga ctcccggcga
      241 gttggagttg acggtcctgg cgttggccag cgtttcgcgc atcgcgtcgg cggagcggtc
      301 ggacgcgccc aggctgaggc cgatccaggc ggcgacgatc caccagaccg cgccgatcag
      361 caggatcgtc gtgttctcgg catcccgaac cccgatcacc gcgatcgccc ctagcgccgt
      421 cgcaccaccg gcagccagga aaaccgtggc cctgagcagc tcggcgaagg acaagcccta
      481 ctccggttgc ggcgcgccgg gctgggcttc cgccggctcc cggctgaagg tgcccgccag
      541 accttcgatc aggtcgatcc actcctccgg ctcggccacg acaaggtccg cgagttcgac
      601 cagttccggc gggccttcgt ccgagtcgat cgcgaccagc agcaggcgct tcagctgacc
      661 ctcatcggca agtttcttga ggcggacggc ggcgtcgtaa tcggtgcggt cgtcaccggc
      721 gaagaccacc gtgtcgatgt cggttccgtc gagcagctcg tcgaccgcgc cggccttgcc
      781 ttccatcccg ggcggacgaa gctcgagtac cttccggccc caatgggtcg caaggccggc
      841 cgactgggcg tggtcggcga tgcccctgat tacttcctcg gtccggtggc cggcgccgcg
      901 ccagtggagg gcctggatcg gacccttgtc ctcgatccgg atcttcgagg tcacccagaa
      961 ggggttgtcg ttctcgcgga tgaagttccg ggcccgctcc gcctcttcgc cctcgatcac
     1021 cacggtcggc cggtcctggc cgggcttgat gaagctgagg ccgtgattgc cgaagtaggt
     1081 gatcttcttg gctccgacca tcttccgggc gaccggcgag gggcgacccg tgatgcaggc
     1141 gaccagcgcg aggtggtcgg ccagggtttc gaggctttcc cgggcccgga ccggaacacg
     1201 ggcctggtcg ggtctgtcga cgatccggca gagcgtgccg tcgacgtcac tcatcaacgc
     1261 cgagttcgcg gggtcttcca gaagtaccga gagaggagag tctgtgcccg tcatatgcga
     1321 gagactaccc cggtccgcct gcgggtcgaa aaagtctcct agtatcgcgc catggacaag
     1381 cggcgcataa tcggcctggc gggagtgggc actctggccg gagcattcag cggactcttc
     1441 ggggtcggag gcggcacggt gatcgtgccg atgctgatcg cgctctatca ctacggcgac
     1501 cgtctggcga ccgg
//
LOCUS       DAOQFI010000590         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_58995_length_1514_cov_2.530500, whole genome shotgun sequence.
ACCESSION   DAOQFI010000590 DAOQFI010000000
VERSION     DAOQFI010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58995_length_1514_cov_2.530500"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..>1514)
                     /locus_tag="PLE93_09580"
     CDS             complement(170..>1514)
                     /locus_tag="PLE93_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HNH87342.1"
                     /translation="VVVGAGLAGLMAARQLAAKGRKVKVLEARGRVGGRTYSPKHGGV
                     TLDVGGQWLGPTQDKVAALADKLGVQTFKTYWEGQAVYQRNGSRTLFNVTSPIPPDPD
                     AAETLNLVLQLESMGAGISRSEPWKDANADLYDGQTFETFKLANTTGEGARFLTDLAT
                     NAVWACEPRDISLLHLLFYISSAGNETTPGSFIRLTSTTEGAQDSRFVGGSQQLSIRM
                     AKQLGSRVQLNAPVRQIRQEKGLCYVTSDKGTWRARQVIVAVPPAVSAFIDYDPILPS
                     DRAQLIQRFPQGNAIKVQAIYDTPFWREEGLSGYVNSDEGPVKLMYDNSPHDGSAGVL
                     LGFIEGQEARLAGRMSPAARKAAVLANFKKTIGNAAGSPKAYVEKNWAEEEWTRGCYG
                     GFLPPGVLTGFGPALRKPHGRIHWAGTEHAEIWNGYMDGALRSGEDAARAALARL"
BASE COUNT          216 a          508 c          520 g          270 t
ORIGIN      
        1 ccgggccgag gtcgccggcg gcgaggtctt cggcgagccg gccatcctca cttcgggagg
       61 cgaaggctgg gacgccctcg aagcctgccg caagcttcag gtcaaggccc tccgcgcctg
      121 accgggcgct cctcgctcct tccccggcgc tcctcgccgg aggtgcgttt cagagtctgg
      181 ccagggccgc cctggcagcg tcttcgccgg aacggagggc gccgtccatg tagccgttcc
      241 agatctcggc gtgctcggtc ccggcccagt ggatgcgacc gtgaggcttg cgcagcgccg
      301 gtccgaaacc ggtcaggaca cccgggggca aaaagccgcc gtagcagccg cgggtccact
      361 cttcctcggc ccagttcttc tcgacgtagg ccttgggtga accggccgcg ttgccgatcg
      421 tcttcttgaa attggcgagc acggcggcct tgcgcgcggc cgggctcatc cgtccggcca
      481 gcctcgcttc ctggccctcg atgaacccga gcaacacgcc ggccgagccg tcgtgggggg
      541 agttgtcgta catcagcttg accgggccct catccgagtt gacgtatccg gagagccctt
      601 cctcccgcca gaacggggtg tcgtagatcg cctgcacctt gatcgcgttg ccctgtggga
      661 agcgctggat cagctgggcc cggtcggaag ggaggatcgg gtcgtaatcg atgaaggcac
      721 tgaccgccgg cggcactgcc acgatcacct gcctggcccg ccaggtgccc ttgtcgctgg
      781 tcacgtaaca aaggcccttc tcctggcgga tctgccggac cggcgcgttg agctgaaccc
      841 gggagccgag ctgtttcgcc atccggatcg aaagctgctg cgagccgccc acgaatcgcg
      901 aatcctgggc gccctcggtg gtcgaggtca gcctgatgaa actgcccggg gtggtctcgt
      961 tgccggccga ggagatgtag aagaggaggt ggagcaggga gatgtcccgc ggctcgcagg
     1021 cccagaccgc attggtggcg aggtcggtca gaaagcgcgc gccctcgccg gtggtgttgg
     1081 cgagtttgaa agtctcgaag gtctggccgt catacaggtc ggcgtttgcg tccttccacg
     1141 gttcggaacg cgagattcct gcccccatgc tctccagctg gagtacgagg ttgagggtct
     1201 cggccgcatc cgggtcgggc gggatcggcg aggtcacgtt gaacagtgtc cggctgccgt
     1261 ttcgctggta gacggcctgg ccctcccagt aggtcttgaa ggtctgcacg ccgagcttgt
     1321 cggctagggc ggcgaccttg tcctgggttg gcccgagcca ctggccgccg acgtcaagcg
     1381 tcacgccgcc gtgcttcggc gagtaggtcc tcccgccgac ccggccccgg gcctcgagga
     1441 ccttgacctt cctgcctttg gcggcgagtt gacgcgcggc catcaatccg gccagtccgg
     1501 ccccgaccac gacc
//
LOCUS       DAOQFI010000591         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_59115_length_1512_cov_4.569664, whole genome shotgun sequence.
ACCESSION   DAOQFI010000591 DAOQFI010000000
VERSION     DAOQFI010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59115_length_1512_cov_4.569664"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLE93_09585"
     CDS             <1..182
                     /locus_tag="PLE93_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH87343.1"
                     /translation="DVQNDEEITGLFENLGKHWDGLDGLVHSIAFAPSEALEGDFLNG
                     LSRSSSRRSTCRRNF"
     gene            164..364
                     /locus_tag="PLE93_09590"
     CDS             164..364
                     /locus_tag="PLE93_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87344.1"
                     /translation="MPQELLTGWIEPVAKVNPVTALLNGARSLMAGDPTGILLAYGIA
                     FGMIALLALWALRGLRRAEVAG"
     gene            449..>1512
                     /locus_tag="PLE93_09595"
     CDS             449..>1512
                     /locus_tag="PLE93_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87345.1"
                     /translation="MSKLEIRRVQSKKDLKKFVKVPFLVHRDHPEWVPPLIMDRMDFL
                     NRDKNPYFDHAEVELWIAERDGEPVGRVSAQIDRNWDEYQGGNVGQFGFFETIDDQEV
                     ASALLDAGCEWLAGQGREKVYGPMDFTTNDEIGIQISGFDIQPSLLENCHQPYFQERV
                     EGAGFSKAMDLLMWHLEMGKLAKGLEFHPAIMESAQQSLDEHGITIRNMRKSDLANEM
                     GRFHEVYNEAWGDNWGFVPITREEVEFHAKTLKMVIDEDWAMIAETADGETVGAALTL
                     PDFNQVLAKMNGRIFPFGWYHFLTGRRKIDSVRILALGVKKAYQHTGVAAALYVKHIQ
                     TTDPNGVMKGEAGWILETNEP"
BASE COUNT          289 a          485 c          483 g          255 t
ORIGIN      
        1 gcgatgttca gaacgacgaa gaaatcaccg ggctgttcga gaacctcggc aaacactggg
       61 atggccttga tggcctcgtg cattcgattg ccttcgcgcc cagcgaggcg ctggagggcg
      121 atttcctcaa cggtctttcg cgctcttcct cgcgccggtc tacgtgccgc aggaacttct
      181 gaccggctgg atcgagccgg tcgcgaaggt caatccggtg accgccctgc tcaacggcgc
      241 ccgcagcctg atggccggcg acccgaccgg gattctgctc gcctacggga tcgccttcgg
      301 aatgatcgcc ctgctggcgc tctgggctct gcgcggactg cgccgggccg aggttgccgg
      361 ctgagtccgt cgcgcggcgg caccgacctc ccggccgcca ggggcggcgc aaccggatcc
      421 cggggtccgg tccgctagtt tcccggcaat gtcgaagctt gagattcgcc gggttcagag
      481 caagaaggac ctgaagaagt tcgtcaaggt ccccttcctc gtccaccgcg accaccccga
      541 atgggtgccg ccgctgatca tggaccggat ggacttcctc aaccgtgaca agaacccgta
      601 tttcgatcac gccgaggtcg agctgtggat cgccgagcgc gacggtgagc cggtcgggcg
      661 ggtcagcgcc cagatcgatc gcaactggga tgaataccag ggcgggaacg tcgggcagtt
      721 cggcttcttc gagacgatcg acgaccagga ggtcgcttcg gccctgcttg acgcgggctg
      781 cgagtggctg gccggccagg gtcgggagaa ggtctacggg ccgatggact tcaccaccaa
      841 cgacgagatc ggcatccaga tttccggctt cgatatccag ccgtcgctgc tcgaaaactg
      901 ccatcagccc tatttccagg aaagggtcga gggtgcgggt ttcagcaagg cgatggacct
      961 gctgatgtgg catctcgaaa tgggcaagct cgccaaggga ctcgagttcc acccggcgat
     1021 catggaatcc gcgcagcaga gtctcgacga gcacgggatc acgatccgca acatgcgcaa
     1081 gtccgacctc gccaacgaga tgggacgctt ccacgaggtc tacaacgagg cctggggcga
     1141 caactggggc ttcgtcccga tcacccgcga ggaagtcgag tttcacgcca agacgctcaa
     1201 gatggtgatc gacgaggact gggcaatgat cgccgaaacc gccgacgggg agaccgtcgg
     1261 cgccgctctc accctgcccg acttcaacca ggtcctggcg aagatgaacg gccggatctt
     1321 cccgttcggc tggtaccact tcctgaccgg tcgcaggaag atcgactcgg tccgcatcct
     1381 cgccctcggg gtcaagaagg cctatcagca caccggcgtc gccgctgccc tctacgtgaa
     1441 acacatccag accaccgatc ccaacggcgt gatgaagggc gaggcgggct ggatcctcga
     1501 aacgaacgag cc
//
LOCUS       DAOQFI010000592         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_59217_length_1511_cov_3.185440, whole genome shotgun sequence.
ACCESSION   DAOQFI010000592 DAOQFI010000000
VERSION     DAOQFI010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59217_length_1511_cov_3.185440"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLE93_09600"
     CDS             complement(<1..181)
                     /locus_tag="PLE93_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87346.1"
                     /translation="MSSIEDAIQQLETSASKLKQLSVEESRAIREAVRVATREATAKV
                     KAEYKDKKDKARKEAR"
     gene            378..>1511
                     /locus_tag="PLE93_09605"
     CDS             378..>1511
                     /locus_tag="PLE93_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH87347.1"
                     /translation="MPPSRFRSSRNPIPHERAFWAMAAIAVTFPAISAVPSPLYSLYQ
                     DMWGFSDAVLTEVFAVYVVALLCSLLVFGSLSDHLGRRPVLLGAIAFEALSLLLFLLS
                     GGVAMLALARVIQGLATGVALATLSAVIVDLQPEDDPKRSGVVNGVAPLAGLAIGALG
                     CGVLIQFAPWPTTLIFLILLAALGFAAVAVWRSPETSTMKPGARESLLPKVGLPAHLR
                     PRFFSLLPIMIAGWALAGLYLSLGPSIASSFIEDDPYLTGAIVVTALCGTGAVVVYRL
                     RHREPVTVIRFAAGWLAAGITINLIGLLLDLAPLALAGTLISGIGFGASALGSFGVLA
                     TLPHPDERGELFAFAYVVSYIAFSVPALIGGFASDRYGLHATAL"
BASE COUNT          194 a          538 c          433 g          346 t
ORIGIN      
        1 ccctggcttc cttgcgggcc ttgtccttct tgtccttgta ctcggccttt accttcgcgg
       61 ttgcttccct ggttgcaacc ctgaccgctt cgcgaatcgc acggctttct tcaaccgaaa
      121 gctgcttgag cttcgaagct gaagtttcaa gttgttgaat tgcgtcttca atgctggaca
      181 tagtctctcc tgttggcgat tgtcatgtgc ctgccagaca tcgtcatgag accggcattc
      241 atgactataa ccaaacgacg ggtttcaaat gtcaattccg gtccgtaacg aaatgtcgtc
      301 ttccggatgt caggcaagtg cgcggttgct caagaggtgc cgttggtacc ttgcctgtct
      361 ccccctgact cgccctgatg cctccttcac gcttccgttc atcccgcaat ccgatccccc
      421 acgaacgcgc cttctgggcg atggcggcga tcgccgtgac cttccccgcg atctcggcgg
      481 tgccttcccc gctctactcc ctctaccagg acatgtgggg cttctccgac gcggtcctga
      541 ccgaggtctt cgccgtatac gtggtcgcgc tgctctgctc cctgctcgtc ttcgggtcac
      601 tctccgatca cctcgggagg cgcccggtgc tgctcggagc gattgccttc gaggcccttt
      661 ccctcctgct gttcctgctt tccggcggag tcgcgatgct ggccctggcc agggtgatcc
      721 agggtctcgc caccggggtg gcgctcgcca cgctcagcgc ggtgatcgtc gacctgcagc
      781 ccgaggatga cccgaagcga agcggcgtgg tgaacggagt ggcgccactc gccggcctgg
      841 cgatcggtgc gctcggctgc ggcgtgctga tccagttcgc cccctggccg accaccctga
      901 tcttcctgat cctgctggcg gcgctggggt ttgccgcggt cgctgtctgg cgctcgcccg
      961 aaacctcgac catgaaaccg ggcgcccgcg agtcactgtt accgaaggtc ggcctgccgg
     1021 cccacctcag gccccggttc ttctcgctgc tcccgatcat gatcgccggc tgggcactgg
     1081 ccgggctcta tctgtcactg ggtccttcga tcgcatcgag cttcatcgag gatgacccct
     1141 acctgaccgg cgcgatcgtg gtcacggcac tctgcgggac cggagccgtg gttgtctatc
     1201 ggctccgtca ccgcgagccg gtgaccgtga tccgcttcgc ggcaggctgg ctggccgccg
     1261 gaatcacgat caatttgatc ggcctgctgc tcgacctggc cccgctcgcc cttgccggga
     1321 ccctgatttc cggaatcggc ttcggagcct cggcgctcgg ttccttcggc gtacttgcca
     1381 ccctccccca tcccgacgaa cgtggcgagc tcttcgcctt cgcctacgtg gtcagctaca
     1441 tcgccttcag cgtcccggcc ctgatcggcg gcttcgcctc ggaccggtac ggcctccacg
     1501 ccaccgccct c
//
LOCUS       DAOQFI010000593         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_59290_length_1510_cov_3.230928, whole genome shotgun sequence.
ACCESSION   DAOQFI010000593 DAOQFI010000000
VERSION     DAOQFI010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59290_length_1510_cov_3.230928"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLE93_09610"
     CDS             <1..672
                     /locus_tag="PLE93_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573412.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNH87348.1"
                     /translation="DQAAVDAALAEVARVAGADDENIMPATIAAAKAGATTGEWAATL
                     REVFGAYRGPTGVGGAAPAGDRDSLTATREKVNEVSGQIGHRIRLLVGKPGLDGHSNG
                     SEQIALRARDVGMEVVYQGIRLTPEQIVESAVQEDVDLIGLSILSGSHLELIPEVVRL
                     LQEEGVEVPVVAGGIIPPEDAARLKEAGVAGVFTPKDFDMNEIMGEFVDLVAANHEKS
                     AAATA"
     gene            685..1278
                     /locus_tag="PLE93_09615"
     CDS             685..1278
                     /locus_tag="PLE93_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNH87349.1"
                     /translation="MNDQLYLLTHGIPGVIAGIMLALTLIGVSGSIWGIKRGLDGGEK
                     ILRVTAAILCLVGIGIAGYVVVTVEIMNQIPECVGGGGGCAAVEHSSYSRIGGIHVSI
                     FGLIGYTMLLVTSFMKGDLARIGAFFLALFGFGFSLYLTYLELWEILAICQWCVSSAI
                     TMTMLFVVNTSRLIVFFGTGESNGLAEGVEDPGPSSV"
     gene            1291..>1510
                     /locus_tag="PLE93_09620"
     CDS             1291..>1510
                     /locus_tag="PLE93_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87350.1"
                     /translation="MLEDQRFIISRDIPIEVGGLFILVALVGIGFAIWGYRTGGPRGE
                     QILRATAGVLSGLGLFVAGYIAYKTQIVN"
BASE COUNT          239 a          465 c          506 g          300 t
ORIGIN      
        1 gaccaggccg cggtcgacgc tgccctggcc gaagtcgcca gggttgccgg cgccgacgac
       61 gagaacatca tgccggccac catcgccgcc gccaaggccg gtgcgaccac cggcgagtgg
      121 gccgccaccc tgcgcgaggt attcggcgcc taccggggtc cgaccggtgt cggaggcgcc
      181 gccccggccg gtgatcgcga ttcgctgacc gcgacccggg agaaggtcaa cgaggtttcc
      241 gggcagatcg gccaccggat tcgcctgctg gtcggcaaac cgggccttga cggccattcg
      301 aacggctccg agcagattgc gcttcgcgcc cgcgacgtgg gcatggaagt cgtctaccag
      361 ggcattcgcc tcactccgga gcagatcgtc gaatcggcgg tccaggagga cgttgacctg
      421 atcgggctct cgatcctttc gggatcgcac ctcgagctga ttcccgaggt cgtgcgactg
      481 cttcaggagg agggcgtcga ggtgccggtg gtcgccggcg gcatcatccc gcccgaggac
      541 gcggcccggc tgaaggaagc cggagtggcc ggggtcttca ctccgaagga cttcgacatg
      601 aacgagatca tgggcgagtt cgtcgacctg gtcgccgcca atcacgagaa gtcggcggcc
      661 gcaacggcct aggcaggagg ttccgtgaat gatcagctct atctgctgac tcacggaatc
      721 cccggtgtaa tcgccgggat catgctggcg ttgaccctga tcggggtcag cggttcgatc
      781 tgggggatca aacgcggcct cgacggcggc gagaagatcc tccgggtcac cgccgcgatc
      841 ctctgcctgg tcgggatcgg aatcgccggt tacgtggtcg tgacggtgga aatcatgaat
      901 cagattccgg aatgcgttgg cggtggcggc ggttgcgccg cggtcgagca cagctcctac
      961 tcgcgaatcg gggggatcca cgtttcgatc ttcggcctga tcggctacac gatgctgctg
     1021 gtcacctctt tcatgaaggg tgacctggcc cggatcggcg ctttctttct ggctctcttc
     1081 ggtttcgggt tcagcctcta cctgacctat ctcgagcttt gggagattct cgcgatctgc
     1141 cagtggtgcg tttcctcggc gatcaccatg accatgttgt tcgtggtcaa tacgagcagg
     1201 ttgatcgtgt tctttggcac cggcgagagc aacggtctgg ctgaaggcgt ggaagaccct
     1261 ggcccgtcgt ctgtgtaggc tcgcttcccc atgctcgaag accagcggtt catcatcagc
     1321 cgggacatcc cgatcgaagt cggcggcctc ttcattctcg tcgctctggt gggcatcggt
     1381 ttcgcgatat ggggttaccg gaccggtggt ccgcgcggcg agcagatcct ccgtgcgacg
     1441 gccggtgtcc tttccgggct cggactgttc gtggctggct acatcgccta caagacccag
     1501 atcgtgaacg
//
LOCUS       DAOQFI010000594         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_59471_length_1508_cov_2.475568, whole genome shotgun sequence.
ACCESSION   DAOQFI010000594 DAOQFI010000000
VERSION     DAOQFI010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59471_length_1508_cov_2.475568"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PLE93_09625"
     CDS             complement(<1..1319)
                     /locus_tag="PLE93_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH87351.1"
                     /translation="MYLRSVTMKGFKSFPEKTELAFSPGVSVIVGPNGSGKSNITDAV
                     LWCLGEQSPGAIRGTSMQDVISVGGKNVGARSEAEVEVVIDNAEGQMGDEFTEISITR
                     KLDRNGDGGYWMNGARCRLSDIIELLSDANLGREMHSVISQGRVDSIVHSKPRERHLL
                     IEEAAGLGKFRKRRRRSELKLLATRDNLDRALDVEREARKALRPLKQQANSAEIGARI
                     ERQELELKSRLVAEDLRFGEARLEQARTAAEKARKVRDGLEGELTAVSRRRRAAEERF
                     AERDRERTEIWGILNRLRSGQEKVSIESSRIAEQGRRLQLRLEDLRLELAPLTLDVGE
                     SGSASERARRLVEELSEVDAGIATAAEGLRQARAEGPEAKRLKALGDVHKGAERATTH
                     ARRAESLLGERHRERLGERMAEVEKLGDALTSLTASADQTGQAIRDRV"
BASE COUNT          206 a          539 c          475 g          288 t
ORIGIN      
        1 acccggtccc gaatcgcctg accagtctga tcggccgagg cagttagtga agtcagggca
       61 tcgcccagct tctcgacctc ggccatgcgc tcacccagcc gctctcgatg cctttcaccg
      121 agcaggctct cggcccggcg ggcgtgggtt gtcgcccttt cggcgccctt gtggacgtcc
      181 cccagtgcct tcagccgctt ggcttccggc ccctcggccc gggcctgccg cagcccctcg
      241 gccgccgtgg caattccggc gtcgacttcg gagagctctt cgaccagccg cctggcccgc
      301 tcgctggcgc tgccgctctc acccacgtcg agggtcagcg gagccagctc gagccggagg
      361 tcttccaggc gaagctgcag tcgccggccc tgctcggcaa tccgcgacga ctcgattgac
      421 accttttcct ggccggaacg cagccggttg aggatgcccc agatctcggt tcgctcgcgg
      481 tccctttcgg caaagcgttc ctcggcggcg cggcggcgcc tggaaaccgc ggtaagttcg
      541 ccttcgagcc cgtcgcggac cttgcgtgcc ttttcggcgg ccgtccgggc ctgttccagg
      601 cgggcttcgc cgaagcggag atcctcggcg accagccggc tcttcagttc gagttcctgg
      661 cgttcgatcc gggccccgat ctcggccgag ttcgcctgct gcttgagcgg cctgagcgcc
      721 ttgcgggcct cgcgctcgac gtcgagagcg cggtcgaggt tgtcgcgggt ggcgagcagc
      781 ttcagctccg agcggcgccg gcgcttgcgg aacttgccga gtccggcggc ctcttcgatc
      841 agcaggtgtc gctcgcgggg cttggagtgg acgatcgaat caacccggcc ctggctgatc
      901 accgaatgca tctcccgccc gaggttggcg tcggagagaa gttcgatgat gtccgacagc
      961 cggcagcggg cgccgttcat ccagtagccg ccgtcaccgt ttcgatccag cttgcgggtg
     1021 atcgagattt ccgtgaactc gtcccccatc tgaccttcgg cgttgtcgat cacgacctcg
     1081 acctcggcct cggagcgggc gccgacgttc ttgccgccga ccgaaatcac gtcctgcatc
     1141 gaggtgccgc gaatcgcgcc cgggctctgc tcgccgagac accagagcac ggcgtcggtg
     1201 atgttcgact tcccggagcc gttcggcccg acgatcacgc tgaccccggg cgagaaggcc
     1261 agctcggtct tttccgggaa ggacttgaag cccttcatgg tcactgagcg cagatacatg
     1321 cgccttacag attgccggtt ccgccggacg gacgattccc caccccggaa cgcctcaact
     1381 ccgcagcccg actgggaaaa accgtttccc gcaaagtgca gggagtttca gtcgccgagc
     1441 ctttcgaggg cgacttcggc ggccgcctga ccggctgctt tcttgcttct gccttcaccc
     1501 ctcccgat
//
LOCUS       DAOQFI010000595         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_59554_length_1507_cov_2.502755, whole genome shotgun sequence.
ACCESSION   DAOQFI010000595 DAOQFI010000000
VERSION     DAOQFI010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59554_length_1507_cov_2.502755"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLE93_09630"
     CDS             complement(<1..680)
                     /locus_tag="PLE93_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH87352.1"
                     /translation="MATLRSRIALALIKKQLPKIVAADPVAELGDGLHVAVVGSGSPL
                     PDDKRGNPCTLVIAGEKVFVVDAGEGASETINRMGIDPGRIEALLLTHFHSDHIGGIP
                     SVNLQRWVADGTDTPMPVLGPPGAERVVKGFNDAYRLDRSYRTAHHGEDYVDPAIGPM
                     AVEEFPVPGEGESIVVLEDDGLVVSAFEVDHSPVAPVVGFRFDYQGRSAVISADTVYS
                     GNLVKAAK"
     gene            complement(714..>1507)
                     /locus_tag="PLE93_09635"
     CDS             complement(714..>1507)
                     /locus_tag="PLE93_09635"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArgP/LysG family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNH87353.1"
                     /translation="QRIKAMERSTGQVLVQRSNPVEPTTAGDIALRYGRQMRLLEDEA
                     ARALALEESGGGAVTVPLANNADSLGTWFMEALARVDSPGVVFEVFREDQEHTTSLLR
                     AGKVMAAVTSNAEAVQGCRSELLGTMTYHPVCSPGFLEGQLGGEATPANLGKATVVDF
                     DRKDQTQNRFFREFTGKPLRAPRHYIPTTADYARAILLGLGWGLLPDRQCLEEIKRGD
                     LVEFAPDQAIEMPLYWQRWTLESPLLEVLTEAVKGVAAREMHPRD"
BASE COUNT          226 a          510 c          506 g          265 t
ORIGIN      
        1 ttcttcgcgg ccttgacgag gttccccgaa tagacggtgt cggcactgat cacggcgctc
       61 cggccctgat agtcgaagcg gaaaccgacc accggcgcga ccggcgaatg gtccacctcg
      121 aaggcggaca cgaccagccc gtcatcttcg aggaccacga tcgattcccc ttcgccgggg
      181 accgggaact cctcgaccgc catcggcccg atcgccgggt caacatagtc ctcgccgtga
      241 tgggcggttc ggtagctgcg gtcgagccgg taggcgtcgt tgaacccctt cacgaccctt
      301 tcggccccgg gcggtcccag gaccggcatc ggcgtgtcgg tgccgtcggc cacccagcgc
      361 tggagattga ccgaggggat gccgccgatg tgatcggagt ggaagtgggt cagcagcagg
      421 gcctcgatcc ggcccgggtc gatgcccatc cggttgatcg tttccgaggc cccttcgccc
      481 gcgtcgacca caaagacctt ctcacccgcg atgaccaggg tgcagggatt gccgcgcttg
      541 tcgtcgggca agggagagcc cgagccgacg accgcgacgt ggagtccgtc gccgagttcg
      601 gcgaccgggt cggccgcgac gatcttcgga agctgcttct tgatcagggc tagggcgatt
      661 cgggagcgta gggtggccat gatccgaatc ctatagttga cgcgcgtcaa tttctagtcc
      721 cgggggtgca tctcccttgc tgcgacccct ttgaccgcct cggtgagcac ctccaggagc
      781 ggcgactcca gggtccagcg ttgccagtag agcggcatct cgatcgcctg gtccggggcg
      841 aactcgacca ggtctccccg cttgatctct tccaggcact ggcggtccgg cagaaggccc
      901 cagcccaggc cgagcaggat cgcccgggcg taatcggcgg tggtcgggat gtaatgccgc
      961 ggcgcccgga gcggcttgcc ggtgaattcc cggaagaaac ggttctgggt ctggtccttg
     1021 cggtcgaagt cgaccaccgt ggccttgccg aggttggccg gcgtcgcttc gccgccaagc
     1081 tgcccttcga ggaagccggg tgaacagacc gggtggtagg tcatggttcc gagcagctcg
     1141 gagcggcagc cctggaccgc ttccgcattc gaggtcacgg ccgccatcac cttgccggcc
     1201 ctgagcaggg aggtcgtgtg ttcctggtcc tcgcggaaga cctcgaagac cacccccggc
     1261 gagtcgaccc gcgccagggc ctccatgaac caggtcccga ggctgtccgc gttgttcgcc
     1321 aacggcaccg tcaccgcgcc gcccccgctt tcttccaggg ccagggcgcg ggcggcctcg
     1381 tcttccagca gtcgcatctg gcggccgtag cggagggcga tgtcgccggc cgtcgtcggt
     1441 tcgaccggat tgctccgctg gaccagcacc tgaccggttg accgttccat cgccttgatc
     1501 cgctgcg
//
LOCUS       DAOQFI010000596         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_59596_length_1506_cov_3.483115, whole genome shotgun sequence.
ACCESSION   DAOQFI010000596 DAOQFI010000000
VERSION     DAOQFI010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59596_length_1506_cov_3.483115"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLE93_09640"
     CDS             complement(<1..1157)
                     /locus_tag="PLE93_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87354.1"
                     /translation="MAGDRIIRGIIAGAATVFVSLFASGSLAQASDFTVTNLAGDNSA
                     GSLHAAMDAANANPSDKDSITFQAGLVGAISLVDDLPELTGPVEINGPGSSKLSIGRD
                     PVNYPFHAFRTSKDLTLTGMTVVDGVDPGNGGTISSTGGDLVLKDVTVTRSRAYYGKG
                     GALCVDGGSLTIEDSTFYDNTAVTGAGAWISNADSVSISNSSFSNNKARYFFAGPHPD
                     PNTGGGLRIEDTAKVEINGSVIQANTADTDGAAFSINNAGEVTLADTFVANNEMAFIE
                     SPPGYDYFGTARIIADQTTITDSQFIGNSSGSILAGLQIIGAAKISGSVIANNVTGGP
                     YSGLILGGDVEHADTNNVTATVSNTTITGNQGGSLGSGLISTVSKLNLNSST"
BASE COUNT          254 a          424 c          492 g          336 t
ORIGIN      
        1 gtcgatgaat tcaggtttag tttcgagacc gtgctgatca ggcccgatcc caggctgcct
       61 ccctgattgc cggtgatcgt cgtgttggaa acggtcgcgg tcacgttgtt ggtatcagcg
      121 tgctcgacgt ctccgccgag tatgaggccg ctgtagggcc cgccggtgac gttgtttgcg
      181 atgacgctgc cggaaatctt tgcggcaccg atgatctgga gaccagcgag gatgctcccg
      241 ctggagttgc cgatgaactg agagtcggtg atcgtggtct ggtcagcgat gatccgggcg
      301 gtaccgaaat agtcgtaacc cggcggggat tcaatgaagg ccatctcgtt gtttgccacg
      361 aaagtgtcgg cgagcgtcac ttccccggcg ttattgatcg agaaagcggc accatcggtg
      421 tcagcggtgt tggcctgaat gaccgacccg ttgatctcga ccttcgccgt gtcttcgatc
      481 cgcagcccgc cgcccgtatt cgggtccggg tggggtcctg cgaagaagta ccttgctttg
      541 ttgttggaga acgaagagtt cgaaatcgac accgaatcgg cgttgctgat ccaggcaccg
      601 gcacccgtga cagcggtgtt gtcgtaaaaa gtcgagtcct cgatggtcag gctgccgccg
      661 tccacgcaaa gcgcaccgcc cttgccgtag taggcgcggg acctggttac cgtgacgtcc
      721 ttcaggacca gatcgcctcc ggtggagctg atcgtgccgc cgtttccggg gtcgactccg
      781 tcgactacgg tcattccggt cagggtcagg tccttgcttg tccggaacgc gtgaaaggga
      841 tagttgaccg gatccctgcc gatggacaac ttgctggagc ccggtccgtt gatctccacc
      901 gggccggtca gctcgggaag gtcatcgacg agggagatcg caccgacgag tcctgcctgg
      961 aaggtgatcg agtccttgtc ggaaggattg gcgttggcgg cgtccatggc ggcgtgaagg
     1021 ctgccggccg aattgtcgcc ggcgaggttg gtgaccgtga agtcgctcgc ctgggcgagt
     1081 gatccgcttg cgaaaaggga aacgaaaacg gtagctgcgc ccgcgatgat cccgcggata
     1141 attctgtctc ctgccactgt gcctgaccaa cttccgtgta ttgggactgg cggtcctgcc
     1201 ggtccctgtt tgttccaaac tagaggaata gtccagactg tcaacccgtc tccggtgtgt
     1261 ccggagcggt tgggaggacc aaagtgtgga agaaggccga cggcccgagc taagtcggcg
     1321 ccagagggga atcctggttc tactttttcg gcttcgccct ggagagggtc accggtttgg
     1381 tcttggtcag aggcttgcct ccggtcgggg tgaacctgac ccgggccttc acttcgacct
     1441 tgcccttctt cctcagggtc ttcagggcct tgcccttcgc cttgatcctg accacggcgg
     1501 ttgagc
//
LOCUS       DAOQFI010000597         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_60123_length_1499_cov_2.468837, whole genome shotgun sequence.
ACCESSION   DAOQFI010000597 DAOQFI010000000
VERSION     DAOQFI010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60123_length_1499_cov_2.468837"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLE93_09645"
     CDS             <1..481
                     /locus_tag="PLE93_09645"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNH87355.1"
                     /translation="DLQRHRMLTCQWQTLTPDLGAGVPAEVVEAGVGGAYEEALEISR
                     NEYERLRDAGHPEAAPYALCLGYRIRYILDLNAREAMQLIELRSGREGHPTYRAVAQA
                     MHDEIAEVHPAVAGAMKHLDTSMEPRLERMLSEIRTHHKQLAAAQTSKAPPGFSELG"
     gene            591..1418
                     /locus_tag="PLE93_09650"
     CDS             591..1418
                     /locus_tag="PLE93_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HNH87356.1"
                     /translation="MLSARIPPALPCALAFLLLAMMVVFAACSSDPDQSEAKPARWQP
                     KPTTAAWQFQLQGKIDTSIDAPVFEVDGFNVPKSTVTRLHSMGRKVICYIDVGSWENY
                     RPDAKRFPGSVIGRKYEGYPNERWLDIRRYRKFAGPIKSRIRMCARKGFDGLEPDNIN
                     GWENPTGFPLTARDQLRFNRWIAKVAHNNGLAVGLKNDGRQARKLVGAFDFAVVEQCF
                     QYSECGQFLPFIRRGKAVYSVEYERPNSAFCARAETLGFSSIGKEYDLFARPWRPCA"
BASE COUNT          268 a          515 c          463 g          253 t
ORIGIN      
        1 ggatcttcag cgccatcgca tgctgacctg ccagtggcag accctgactc cggatctcgg
       61 ggccggggta ccggccgaag tggtggaagc cggggtcggc ggtgcctacg aagaggcgct
      121 cgagatctcg cgaaacgagt acgagcgact gcgcgacgcc ggccatcccg aggccgcccc
      181 ttacgcgctc tgcctcggct accggatccg ttacatcctt gacttgaacg ccagggaggc
      241 gatgcagctg atcgagctcc gatcgggcag ggaaggccat ccgacctacc gggcggtcgc
      301 ccaggcaatg catgacgaga tcgccgaagt ccatcccgcc gtcgccggtg cgatgaagca
      361 cctcgacacc tcgatggagc cccgcctcga gcgaatgctc tccgagatcc ggacccacca
      421 caagcaactc gccgccgccc agacctccaa ggccccgccc ggcttctccg aactcggctg
      481 atccggccag cggccaaacc ggccgcgaga tccgactttt tcgtcacgat ggcgaagaac
      541 tcacgcctca ccgtccccgg ccggtgtgcc cgattcccta acctgtcccg atgctctccg
      601 cccggattcc cccggcgctt ccctgcgcgc tcgccttcct gctgctggcg atgatggtcg
      661 tattcgcagc ctgttcttcc gaccccgatc agtccgaggc gaagccggcc cgctggcagc
      721 cgaaaccgac gaccgcagcc tggcagttcc agctccaggg caagatcgac acctcgatcg
      781 acgcgccggt attcgaggtc gacggcttca acgtgccgaa gtcgaccgtg accaggcttc
      841 actcgatggg tcgcaaggtg atctgttaca tcgacgtcgg cagctgggag aattaccgcc
      901 ccgacgcgaa acggttcccc gggtcggtta tcggcaggaa gtacgaggga tacccgaacg
      961 agcgctggct cgacatccgc cgctatcgga agttcgcggg gccgatcaag tcaaggatcc
     1021 ggatgtgcgc ccgcaaggga ttcgacgggc ttgagccgga caacatcaac ggctgggaga
     1081 acccgaccgg attcccgctg accgcccggg accagcttcg attcaaccgg tggatcgcga
     1141 aggtcgcgca taacaacggc cttgccgtcg gcctcaaaaa cgacggacgg caggcgcgca
     1201 agctggtcgg cgccttcgat ttcgccgtgg tcgagcaatg cttccagtac agcgagtgcg
     1261 gccagtttct gcctttcatc cgtcgtggca aggccgttta ctcggtcgaa tacgaacggc
     1321 ccaattccgc cttttgcgct cgtgcagaga cgcttgggtt tagttcgatt ggcaaggagt
     1381 acgacctctt cgcacggccc tggcgcccct gcgcctagcg gtcacgccgc gggatcgggt
     1441 gagcatcgcc gggacggacg cacgcgcggc attcagccgc gctggtcctg gagtgtccc
//
LOCUS       DAOQFI010000598         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_60269_length_1497_cov_2.667129, whole genome shotgun sequence.
ACCESSION   DAOQFI010000598 DAOQFI010000000
VERSION     DAOQFI010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60269_length_1497_cov_2.667129"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1113)
                     /locus_tag="PLE93_09655"
     CDS             complement(49..1113)
                     /locus_tag="PLE93_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH87357.1"
                     /translation="MILASIFPAQLTALRATGLVIAVLIIAFAIIRRRELRNADVILL
                     LLAGIGLAIVSGTELTDRLLSALSFNRGNGGRILGLAVIAIFILFLMALRALSVGARN
                     ARQLSAVLEGISSEQFRVADNRKKFKGKVAIVIPAYNEADNIGYVLDQIPAEVCGLPT
                     ATLVVDDGSRDGTEEVAEAHGAVVIRHVVNRGGGAALRTGYRLMVDSEAAIVVTLDAD
                     GQHLPAEMPNLVQPVLDGKADVAHGSRVLGHAEDNHFAREMGIVFFNRLVSFITRTHV
                     TDCSNGYRAVRTSVLPTLVLKQEQFHTSEFMIEAIKRGVPAVEVPVTVEQRLHGHSKK
                     PAVFRYGMGFANAIVRTWLR"
     gene            complement(1110..>1497)
                     /locus_tag="PLE93_09660"
     CDS             complement(1110..>1497)
                     /locus_tag="PLE93_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87358.1"
                     /translation="GKENLKEYLGDHPVGFVGMTFRKVGRMWSSGVGDALSSPIGRAI
                     QILLVLAGLAGLVLLAWRRRWEAIAVGLPIATVTAVAAITLAPARRNEILMTLVLPLA
                     AVALDALVNREPKPAGPASDAATETP"
BASE COUNT          262 a          467 c          535 g          233 t
ORIGIN      
        1 ggtcctggca gccgtggggt cggggacccc gcccgacttg cagttaagtc agcgaagcca
       61 ggtgcggacg atggcgttgg cgaagcccat tccgtagcgg aagacggcgg gcttcttcga
      121 gtggccgtgc aggcgctgtt cgaccgtgac cgggacctcg accgcgggca cgccccgctt
      181 gatcgcctcg atcatgaact cggaagtgtg gaactgctcc tgcttgagca ccagggtggg
      241 gaggaccgaa gtccgcaccg cccggtagcc gttggaacag tcggtgacat gggtccgggt
      301 gatgaaggag accagccggt tgaaaaagac gatgcccatt tcccgggcga agtgattgtc
      361 ctcggcgtga ccaagcaccc ggctgccatg ggcgacgtcg gccttgccgt cgaggaccgg
      421 ctggaccagg ttcggcatct cggccgggag gtgctggccg tcggcgtcga gggtgaccac
      481 gatcgccgct tcggaatcga ccatcagccg gtagccggtg cgcagtgcgg cgccgccacc
      541 gcggtttacg acatggcgga tcacgaccgc cccgtgggct tcggcgactt cctcggtgcc
      601 gtcgcgtgaa ccgtcgtcga cgaccagcgt ggcggtcgga agtccgcaga cctcggccgg
      661 gatctgatcg agcacgtatc cgatgttgtc cgcctcgttg taggcgggga tcacgatcgc
      721 caccttgccc ttgaacttct tgcggttgtc ggcgacccgg aactgctcgg aagagatgcc
      781 ttcgagtacg gccgaaagct ggcgggcgtt tctggctccg accgagagcg cccgcagcgc
      841 catcaggaag aggatgaaga tcgcgatgac cgcgagaccg aggatccggc cgccgttgcc
      901 gcggttgaag gagagggcgg agagcaggcg gtcggtgagt tcggtgccgg agacgatggc
      961 caggccgatt ccggccagga gcagcaggat cacgtcggcg ttcctcagct cgcgacgcct
     1021 gatgatcgcg aaggcgatga tcaggaccgc gatcacgagg ccggtcgccc gcagggcggt
     1081 gagctgggca gggaatatcg aggcgaggat catggggttt cggttgcggc atcggatgcc
     1141 gggccggccg gcttcggctc ccggttgacc agggcgtcga gcgcgaccgc cgccagcggc
     1201 aggacgagcg tcatcaggat ctcatttcgc cgggcgggag cgagtgtgat cgccgcgact
     1261 gcagtaaccg tggcaatcgg caagccaacc gcgatcgcct cccagcgccg gcgccaggcc
     1321 agcaaaacca ggccggccag cccggcgagg accagcagga tctgaatcgc ccggccgatc
     1381 ggcgaggaca acgcatcgcc aaccccggag ctccacatcc ggccgacctt gcggaaggtc
     1441 atgccgacaa aaccgaccgg gtgatcgccg agatactcct tcaggttctc cttgccg
//
LOCUS       DAOQFI010000599         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_60397_length_1495_cov_3.418056, whole genome shotgun sequence.
ACCESSION   DAOQFI010000599 DAOQFI010000000
VERSION     DAOQFI010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60397_length_1495_cov_3.418056"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /gene="tmk"
                     /locus_tag="PLE93_09665"
     CDS             complement(<1..340)
                     /gene="tmk"
                     /locus_tag="PLE93_09665"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNH87359.1"
                     /translation="MFITLEGVDGSGKTTQAAMLADALGASAMTLREPGGTDAAERIR
                     QLLADPETPLDPIAELMLFCAARADLVSRVIRPALEIGRTVICDRFTDSTIAYQGVAR
                     GLGIVRVRELS"
     gene            complement(344..>1495)
                     /locus_tag="PLE93_09670"
     CDS             complement(344..>1495)
                     /locus_tag="PLE93_09670"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNH87360.1"
                     /translation="ELIVRRFLATFGEPMVTESTRADIEAGSETYFVRGKVVLDPGYA
                     GIYTYARSADEEIPALEEGQQLAIEGSSWLVDKETQPPARLSEAKLVQLMDEQGLGTK
                     ATRPDIIQKLYNRRYVRNHPPEPTATGIAMYEAFKEYVPDMATSAMTASLEGEMDEIA
                     EEKMTKDEVVEDSRKLVHKTFKDLLQGEEALSKMIWEGMDQDRILGPCIVCKEAGRTK
                     EDGSPNVLRIIRAKKSGKSFVGCTGWEADNPESCDQTFPMPQPNFYEVTPLEENCSVC
                     HRTPRVNVKTRGRAGRPWKLCFNDDCPTMVEMREKKAERLAAQEAAKKAKELDEAEAK
                     KSGKKKPAKKSGARKKSSKTKTVAARIANPPDLGTRRVKKAGARKPKKG"
BASE COUNT          191 a          494 c          496 g          314 t
ORIGIN      
        1 cggagagctc gcgaaccctg actatcccca ggccgcgggc caccccctgg taggcgatgg
       61 tcgagtcggt gaagcgatcg cagatcacgg tccggccaat ctccagggca ggccggatca
      121 cccggctgac caggtcggcc cgggcggcgc agaagagcat cagttcggcg atcgggtcga
      181 gcggggtctc cgggtcggcc aggagctgcc ggatccgctc cgcggcgtcg gttccgccgg
      241 gctcgcgcag ggtcatcgcg ctcgctccga gagcgtcagc cagcatcgcg gcctgggtgg
      301 tcttgcccga gccgtcgacc ccttcgagcg tgatgaacac gcgtcagccc tttttgggct
      361 tgcgagctcc cgccttcttc acccggcggg tgccgaggtc gggcgggttg gcgatccggg
      421 cggcgacggt tttcgtcttc gaggatttct ttctggcgcc ggacttcttg gccggcttct
      481 tcttgccgga cttcttcgct tcagcttcgt ccagttcctt cgccttcttc gccgcttcct
      541 gggccgcgag cctttcggcc tttttttcgc gcatctccac catggtcggg cagtcgtcgt
      601 tgaagcagag cttccacggc cggccggccc ggccccgggt cttgacgttt acccgcgggg
      661 tgcggtggca gaccgagcag ttctcttcca gcggcgtcac ttcgtagaag ttcggctgcg
      721 gcatcgggaa ggtctggtcg caggattccg ggttgtcggc ttcccagccg gtgcagccga
      781 cgaacgactt gccggacttc ttggcccgga tgatccgcag cacgttcggc gagccatcct
      841 ccttggtccg tccggcttcc ttgcagacga tgcagggccc gaggatccgg tcctggtcca
      901 tgccttccca gatcatcttc gagagggcct cttcaccctg gagcagatcc ttgaaggtct
      961 tgtggaccag cttgcggctg tcctcgacga cctcgtcctt ggtcatcttc tcttcggcga
     1021 tctcgtccat ctcgccttcc agcgaggcgg tcatcgcgga ggtcgccatg tccggcacgt
     1081 actccttgaa tgcctcgtac atcgcgatgc cggtcgcggt cggctccggc ggatggttcc
     1141 gcacgtagcg ccggttgtag agcttctgga tgatgtcggg ccgggtcgcc ttggtgccga
     1201 ggccctgttc gtccatcagc tggaccagct tcgcctccga gaggcgggcg ggcggctggg
     1261 tttccttgtc gaccagccac gacgaaccct cgatcgcgag ctgctggccc tcttcgagag
     1321 ccgggatttc ctcgtcggcg gagcgcgcgt aggtgtagat gccggcgtaa ccgggatcga
     1381 gcacgacctt gccgcggacg aagtaggtct cggagccggc ctcgatgtcg gcccgggtcg
     1441 attcggtcac catcggctcg ccaaaggtgg cgaggaagcg ccggacgatc agctc
//
LOCUS       DAOQFI010000600         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_60409_length_1495_cov_3.029167, whole genome shotgun sequence.
ACCESSION   DAOQFI010000600 DAOQFI010000000
VERSION     DAOQFI010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60409_length_1495_cov_3.029167"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PLE93_09675"
     CDS             <1..947
                     /locus_tag="PLE93_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNH87361.1"
                     /translation="GMAWPCIENARAAPKGGWPIISYAHGTTGIADKCAPSRNTKGGV
                     ADPYISYMDPLQNAWLRAGYAVARTDYQGLGVPGVNHPFLVGVPAGRSVLDIVSASRQ
                     LGLKIGNRYLIAGHSQGGHAALFAAGLASSYSKDIKLRGTVSYAPASHFKEQASFLPG
                     LTSPNGLTALATLLVRGMSISYPQLNANEALSDTILPFYPQTLTKCLSELAGSDNLGG
                     IAPSELIRSGYDMTPLNGILEAQNPSVTTSAPILLAQGTADSTTFPFLTDQLDTELVG
                     KGDTVDYKKYEGVDHGGVVAAAQNDVMSFFESRLPSGK"
     gene            complement(1000..1428)
                     /locus_tag="PLE93_09680"
     CDS             complement(1000..1428)
                     /locus_tag="PLE93_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNH87362.1"
                     /translation="MRCMVIVKATEDSEADVMPSEKDLGDMGSFNEELVNAGVMLDGD
                     GLKSSRHGVRITYSGDEAQVTDGPFSETKELIAGYWVWEVKSLEEAIEWARRIPFHHG
                     ESVEIRPFHELEDFGEAVTDEFRERQARMEATIEEQKRDA"
BASE COUNT          257 a          521 c          432 g          285 t
ORIGIN      
        1 tcggcatggc gtggccctgc attgaaaacg caagagccgc cccgaagggc ggctggccga
       61 tcatcagtta cgcccacggc accaccggca tcgccgacaa gtgcgccccc tcgcgtaaca
      121 ccaagggtgg cgtcgccgac ccttacatct cgtacatgga tccgctgcag aatgcctggc
      181 tgagggccgg ctacgcggtt gcccgcaccg attatcaggg ccttggcgtg cccggtgtga
      241 accacccgtt cctggtcggc gttccggccg gccgcagcgt gcttgacatc gtcagcgcct
      301 cgcgtcagct cggtctgaag atcggcaaca ggtacctgat cgccggccat tcgcagggcg
      361 ggcacgcggc actgttcgcc gccggtcttg cctcgagcta ctcgaaggac atcaagctga
      421 ggggcaccgt ttcctacgca ccggcttcgc acttcaagga gcaggcctca ttcctcccgg
      481 gactcacctc ccccaacgga ctgaccgccc tggccacgct gctggttcgc ggcatgagca
      541 tctcctaccc gcagctgaac gcgaatgaag cgctttccga cacgattctg cccttctacc
      601 cgcagacgct gaccaagtgc ctgtcggagc tcgcagggtc tgacaacctc gggggcatcg
      661 cgccttccga gctgatccgc tcgggctatg acatgacccc gctcaacggg atcctcgaag
      721 cgcagaaccc gtcggttacg acttcggctc cgatcctgct ggcgcagggc acggccgatt
      781 cgaccacctt cccgttcctc accgaccagc tcgacaccga gctggtcggc aagggcgaca
      841 cggtcgacta caagaagtac gaaggcgtcg accacggcgg agtcgtggca gccgcccaga
      901 acgacgtcat gtcgttcttc gaatcgcgac tgccgtccgg caagtaggtc cggccggcag
      961 aagttctgaa agagggggcc tccgggcccc ctcttttcgt caggcgtccc gcttctgctc
     1021 ttcgatcgtt gcttccatcc gggcctgccg ttcccggaac tcgtcggtta ccgcctcgcc
     1081 gaagtcttcg agttcgtgga agggccggat ctcgaccgac tcgccgtgat ggaaggggat
     1141 ccggcgtgcc cactcgatcg cttcctcgag cgacttcacc tcccagaccc agtatccggc
     1201 gatcagctcc ttggtctcgg agaagggacc gtcggtcacc tgggcttcat ctccggagta
     1261 ggtgatccgg accccgtgac gggatgactt caatccgtcg ccgtccagca tgaccccggc
     1321 gttgaccagc tcctcgttga aactccccat gtcacccagg tccttctcgg atggcatcac
     1381 atctgcttcg gaatcctcgg ttgccttgac gatcaccatg caacgcatct caatctcctt
     1441 cggtagacgg ccggcttttc cggcctttgc atcgaagacg aacgggaagg ctgga
//
LOCUS       DAOQFI010000601         1491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_60745_length_1491_cov_1.634401, whole genome shotgun sequence.
ACCESSION   DAOQFI010000601 DAOQFI010000000
VERSION     DAOQFI010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60745_length_1491_cov_1.634401"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLE93_09685"
     CDS             complement(<1..349)
                     /locus_tag="PLE93_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH87363.1"
                     /translation="MIHKALGYWIHEAGPQPGFPGLQGDQDFDVIVVGGGFTGLWSAF
                     HLKTAVPELKVGLLESHRIGHGPSGRNGGFVDAMWVSFASLVKRYGSEPALRLARASE
                     QSVRQVGQFCEEHE"
     gene            complement(423..>1491)
                     /locus_tag="PLE93_09690"
     CDS             complement(423..>1491)
                     /locus_tag="PLE93_09690"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH87364.1"
                     /translation="ATALQFLPIMVLGAWGGSLADRFAKRDLLMVTQALMMIPPLIML
                     GVTLTGIVTPAMVLGLILVRGTLLAVDNPARQAFVMEMVGPDRIVNAVSLNSVIIHSA
                     RVAGPALAGILIATVGVAPCFGLNAATFLVMIWALHGMDPKRLSQPPKADREPGRVRA
                     AIRHVRNTPELAMPLFLMAVVGTLGLNFQVILPLLARLTFEGGASSYAVLVSSMGAGS
                     VIGALITGARGKTGMGVIAMSALAFGGLAMAAAVMPSLASEIPILALLGASAVTFAAA
                     INSTIQLAVEPEMRGRVMALYSVVFLGSTPIGGPLVGFISESADPRLALALASISGLI
                     AAAGAWRISNQGQRREALEAA"
BASE COUNT          262 a          521 c          500 g          208 t
ORIGIN      
        1 cctcatgttc ctcgcagaac tgccccacct gccgcaccga ctgctcggat gcccgggcga
       61 gccggagcgc cggttcgctg ccgtagcgct tgaccaggct ggcgaaactg acccacatcg
      121 cgtcgacgaa gccgccgttt cggccactgg gcccgtggcc gatgcggtgg gattcgagca
      181 gaccgacctt gagctccggt acggccgttt tcaggtgaaa ggcactccag agtccggtga
      241 aaccgccgcc gaccacgatc acgtcgaagt cctggtcgcc ttgaagcccg gggaagccgg
      301 gctgcggccc cgcctcgtga atccagtatc cgagtgcctt gtggatcacc tcggcgcagg
      361 ctaccggtag gccgattcgt ccgcccgcga gttcctcaac cggaccgcgg agcccggccc
      421 gctcaggcgg cttcgagcgc ttcccgccgc tggccctggt tgctgatccg ccaggcgccg
      481 gcggccgcga tcagtcccga gatcgaggcc agagcgaggg ccagcctcgg gtctgccgac
      541 tcggagatga aaccgaccag cggcccgccg atcggggttg acccgaggaa gaccaccgag
      601 tagagggcca tcacccgtcc cctcatttcg ggttccaccg ccagctggat cgtcgagttg
      661 atcgccgccg cgaaggtcac cgcggatgcc ccgagcaggg ctaggatcgg gatctccgag
      721 gcgaggcttg gcatgaccgc cgccgccatc gcgagacctc cgaaggcgag cgccgacatc
      781 gcgatcactc ccatgccggt cttgccgcgg gctccggtga tcagggcccc gatcaccgag
      841 ccagccccca tcgaggagac cagaaccgca tacgaactgg cgccgccctc gaaggtgagc
      901 cgggccagga gcggcaggat cacctggaag ttcaggccga gcgtgccgac caccgccatc
      961 agaaaaagcg gcatcgccag ctcgggggtg ttccggacgt gacggatcgc cgcccggacc
     1021 cggcccggct cacggtccgc cttcggcggc tggctcagcc gcttcggatc catgccgtga
     1081 agcgcccaga tcatgaccag gaaagtggcg gcgttgagac cgaagcaggg cgccaccccg
     1141 accgtggcga taaggatgcc ggccagcgcg ggcccggcga cgcgggccga gtggatgatc
     1201 acgctgttca ggctgaccgc gttgacgatc cggtcggggc cgaccatctc catcacgaac
     1261 gcctgccggg ccgggttgtc cacggcaagc agggttcccc gtacgaggat cagcccgagg
     1321 accatcgccg gcgtcacgat tcccgtcaag gtgacgccga gcatgatcag cggcgggatc
     1381 atcatcaggg cctgggtgac catcagaaga tcacgcttcg cgaagcggtc agccagcgaa
     1441 ccgccccagg cgccgagcac catgatcggg aggaactgaa gggcggtggc g
//
LOCUS       DAOQFI010000602         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_60805_length_1490_cov_2.243206, whole genome shotgun sequence.
ACCESSION   DAOQFI010000602 DAOQFI010000000
VERSION     DAOQFI010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60805_length_1490_cov_2.243206"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLE93_09695"
     CDS             complement(<1..1233)
                     /locus_tag="PLE93_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HNH87365.1"
                     /translation="MKKFLVLAAALLALAFTAQTAQAEPEHELVFGEITVEVHGGGTI
                     TGTGINCGADCYDDDSWYDNEIPPTNRLTATAGRGWAFGGWEGCTSVNGQSNKCDAGY
                     QEFGGTPVIASFYDVMAPSVFLQFPALNTVANDTLHAAVESGDNDRVEKVEYLIDGQV
                     AKTVDSGGIDVYIDVTGVSEGQHEIQARAYDPTGNYGITAARTFTVDHSGPDITLNAP
                     LVATNADSPAFSFSTTATDVKNTWCAIVPKGDDVNYSACSPNEPFVGPGLAEGEWIFT
                     VWATDNVGNLTQITHDFVVDRTAPVADFTSGPADGATVKPGEVTYGWSATDGLAVAQH
                     CSWDGAEATGCEGTASKNLAAGSHNFQVTIVDQAGNETTLSRSVTVKKATGPDPDPDP
                     DPDPDPDTTDRTAPVIKLA"
BASE COUNT          233 a          440 c          521 g          296 t
ORIGIN      
        1 cgccagcttg atgaccggtg ccgtccggtc cgtggtgtcc ggatccgggt cggggtcggg
       61 gtcgggatcc gggtcgggac cggtcgcctt cttcacggtg accgaacgcg agagcgtcgt
      121 ctcgtttccg gcctgatcga cgatcgtgac ctggaagttg tgcgaaccgg cggcgaggtt
      181 cttcgacgcg gtgccttcac aaccggtcgc ctcggcgccg tcccacgagc agtgctgggc
      241 aacggccagg ccgtcggtcg ccgaccagcc gtaagtgacc tcgcccggct tcacggtcgc
      301 gccgtcggcg gggcccgaag tgaaatcggc gacgggcgcg gtgcggtcga ccacgaagtc
      361 gtgggtgatc tgggtcaggt tgccgacgtt gtcagtcgcc cacacggtga agatccactc
      421 gccctcggcg agacccggtc cgacaaacgg ttcgttcgga ctgcaggcgc tgtagttgac
      481 gtcgtcgccc ttcgggacga tcgcgcacca ggtgttcttg acatcggtgg cagtggtact
      541 gaacgagaag gccggcgagt cggcgttggt cgcaaccagc ggagcgttca aggtgatgtc
      601 cggaccgctg tgatcgacgg tgaaggtgcg ggcggcggtg attccgtagt tgccggtcgg
      661 gtcgtaggcg cgggcctgga tctcgtgttg gccttcggaa actccggtga cgtcgatgta
      721 gacatcgatg ccgccgctgt ccacggtctt ggcaacctgg ccgtcgatca ggtactcgac
      781 cttttcgacc cggtcgttgt caccggattc aaccgcggcg tggagcgtgt cgttggcgac
      841 cgtgttcagg gccgggaact gcaggaacac gctcggtgcc atcacgtcat agaagctggc
      901 gatgaccggg gtgccgccga attcctgata gccggcgtcg cacttgttcg actggccgtt
      961 gaccgaggtg caaccctccc agccgccgaa ggcccagccg cggccggccg tcgcggtgag
     1021 tcggttggtc ggcgggatct cgttgtcgta ccacgagtcg tcgtcgtaac agtcggcgcc
     1081 gcagttgatg ccggtaccgg tgatcgtgcc accgccgtgg acctcaaccg tgatctcgcc
     1141 gaagacgagt tcgtgttccg gttcggcctg agccgtttgc gcagtgaatg cgagggccag
     1201 caaggccgcc gccaggacga ggaacttctt cattggattg ctcctgttga ttttcggggg
     1261 aaaggggaaa ttcacgatgg cggcaggttt ccgggaagcc ctttccgtct tgttgcggaa
     1321 actttgcggg gttcgcaagg ttcgggccga cgcccatccg aatttcaggt agaaagccga
     1381 aaaagccgag gcagacggct cgctagtgtc tgtgtatggc cgagcttccc gatcgccagg
     1441 aaagcctcga actccttggt cgcatgtgcc tgatccggcg cttcgaggag
//
LOCUS       DAOQFI010000603         1486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_61097_length_1486_cov_2.698812, whole genome shotgun sequence.
ACCESSION   DAOQFI010000603 DAOQFI010000000
VERSION     DAOQFI010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61097_length_1486_cov_2.698812"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1486)
                     /locus_tag="PLE93_09700"
     CDS             complement(<1..>1486)
                     /locus_tag="PLE93_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87366.1"
                     /translation="MAQNRNHYFALKNGRLFVKPNYERTGRRGSWRHVTTPSCLDGDI
                     RSIDADDDELTAINSQNQIFGMDQALSSPSRFNWTSRWGFPIWLGDGYTIPKDTVAWA
                     WTVISPRESRYWVDPAGNQHTVGEGKVSHVLSLRGDHRRITMNDPWLPQDRSYEVCGP
                     RRGTFRISNLAASGSVLFVIGDHGDMYTRLWDFDIAGLDNAFFDYSYEDQRGVEDPAI
                     QLPGAHWVQQPKIRGEITNRISITTIGRGTLHRILRVEGKNAKGLPGFWQKDLYTLNR
                     GSSGWKFVATPKAPITGRWLDNPPRDMSAVGAGRPEGRRYFASGANYLAEIPDFNVSC
                     GRALLRVSFDNGKSIDLNLFLDDMVRIYPRARGLDGEPRLVLGTIGVTPRARATGGDE
                     ARDFIRDVLGDRKFTTARITATTSGLEIPDLGLQFTAAGEPSAGRVPDQGFKEFHVMG
                     RKLGLAFSFETATESPASIVISRIDGDRRGPVAARLTRTGSFFWE"
BASE COUNT          236 a          464 c          519 g          267 t
ORIGIN      
        1 gctcccagaa aaacgacccg gtgcgggtga ggcgggctgc caccggtccg cgacggtcac
       61 cgtcaatccg gctgatcacg atcgaagccg ggctttccgt cgccgtctcg aaggagaagg
      121 cgagccccag cttccgaccc atcacgtgga actccttgaa gccctggtca ggtacccgtc
      181 cggccgaagg ttccccggcg gctgtgaact gaaggccgag gtccggaatc tcgagcccgg
      241 aggtcgtcgc ggtgatccgc gcggtggtga atttgcgatc gccgagcacg tcccggatga
      301 agtcgcgggc ttcgtcaccg ccggtcgccc gggcccgtgg cgtgaccccg atggttccca
      361 ggacgagccg tggttcgccg tcgaggccgc gggcgcgcgg gtagatgcgg accatgtcgt
      421 cgaggaagag attcaggtcg atgctttttc cgttgtcgaa gctgacccga agcagggccc
      481 gcccgcagga aacgttgaaa tccgggatct cggccaggta gttggctccg ctcgcgaagt
      541 agcgccggcc ttcgggccgg cccgccccga ccgcactcat gtcccggggc ggattgtcaa
      601 gccagcgacc ggtgatcggc gccttcgggg tggcgacgaa tttccagccg gacgagcccc
      661 ggttcagggt gtagaggtcc ttctgccaga acccgggcag tcctttcgcg tttttgccct
      721 cgacccggag gatgcggtgc agggtgcccc ggccgatcgt ggtgatgcta atccggttgg
      781 tgatctcgcc ccggatcttc ggttgctgga cccagtgggc gccgggcagc tgaatcgccg
      841 gatcctcgac cccgcgctgg tcctcgtagg agtaatcgaa gaaggcgttg tcgaggccgg
      901 cgatgtcgaa gtcccagagc cgggtgtaca tgtcgccgtg gtctccgatc acgaaaagca
      961 ccgaaccaga ggcggcaagg ttgctgatcc ggaaggttcc gcggcggggg ccacagacct
     1021 cgtaactccg gtcctggggc agccaggggt cattcatggt gatccgccga tggtccccgc
     1081 gcaggctgag cacatgcgag accttgccct caccgaccgt gtgttggttg ccggccgggt
     1141 cgacccagta cctcgattcc cgcggcgaga tcacggtcca ggcccaggcc acggtgtcct
     1201 tggggatcgt gtagccgtca cccagccaga tcgggaagcc ccagcgggag gtccagttga
     1261 agcgggaggg gctcgaaagc gcctggtcca tgccgaagat ctggttctgc gagttgatcg
     1321 cggtcagctc gtcatcgtcg gcgtcgatcg agcggatgtc gccgtccagg cacgacgggg
     1381 tggtgacgtg ccgccagctc ccgcgcctgc cggtccgctc gtaattcggt ttgacgaaga
     1441 gccggccgtt tttgagggcg aagtagtgat tgcggttctg ggccat
//
LOCUS       DAOQFI010000604         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_61463_length_1481_cov_3.694250, whole genome shotgun sequence.
ACCESSION   DAOQFI010000604 DAOQFI010000000
VERSION     DAOQFI010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61463_length_1481_cov_3.694250"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PLE93_09705"
     CDS             <1..1136
                     /locus_tag="PLE93_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH87367.1"
                     /translation="VPESPRWLFIHGREKEADRIVSDIEREVERETGRQSLEEPEEMI
                     EVEQRKTISLGEVASTAFRKYPKRTALGLALFVGQAFLYNAITFDLGTLLGTFYGVAS
                     GTVPIFIAVYALSNFAGPLVLGRLFDTVGRKPMIVLTYGIAAAGGLALGLLFTQDGFD
                     KYWFMALLVTIFFFASAGASSAYLTVSEIFPMETRALAIAFFYAVGTGLGGIVGPLLF
                     GHLIETGDRSTMAIGFYLGAGIMLIGALAELIFGVKAEKQPLEQIARPLTATSDQVEA
                     ESGPKRELPRRRRTGRFRPGPGTLVMARGYPLTAGDEVEAAVEVEALVAILAEQGEMS
                     REELARKAGARQWGPGRFGYVLAQAVAGGRVVRSGRRVSLPSG"
     gene            complement(1138..>1481)
                     /locus_tag="PLE93_09710"
     CDS             complement(1138..>1481)
                     /locus_tag="PLE93_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH87368.1"
                     /translation="VTVLTGVTPENDAYREEFFGPVATVYKVASEDEAVELANDTPFG
                     LGSYVFTDDPEQARRVAENLDTGMVYVNGVGLDGAELPFGGVKRSGFGRELGTYGIQE
                     FINKKLIRTLK"
BASE COUNT          243 a          484 c          485 g          269 t
ORIGIN      
        1 acgtgcccga gagccctcgc tggcttttca tccacggacg ggaaaaggaa gccgaccgga
       61 tcgtcagcga catcgaacgg gaagtcgaac gcgaaaccgg ccgccagtcg ctggaggagc
      121 cggaagagat gatcgaggtg gaacaacgca agacgatctc cctcggggaa gtcgcctcga
      181 ccgcctttcg caagtacccg aaacgaacag ccctggggct cgccctgttc gtcggccagg
      241 cctttctcta caacgcgatc acctttgacc tcgggaccct gctcggcacg ttctacggtg
      301 ttgcctccgg caccgtgccg atcttcatcg cggtctatgc cctgagcaat ttcgccgggc
      361 cactggttct gggtcgcctc tttgacacgg taggccgcaa gccgatgatc gtgctcacct
      421 acgggatcgc cgccgccggc ggacttgccc tcggcctgct gtttacccag gacggcttcg
      481 acaagtactg gttcatggcg ttgctggtca cgatcttctt cttcgcctcg gccggggcca
      541 gctcggccta cctgacggtg agcgagatct ttccgatgga gacccgggcc ctggcgatcg
      601 ccttcttcta cgcggtcggt accggcctgg gcgggatcgt cggcccgttg ctcttcggcc
      661 acctgatcga aaccggtgac cggagcacga tggcgatcgg cttctatctc ggcgccggaa
      721 taatgctgat cggtgccctg gccgaactga tcttcggggt caaagccgaa aagcagccgc
      781 tagagcagat cgccaggccg ctgaccgcca cttcggatca ggtggaggcc gaaagcggtc
      841 caaaacgcga acttccccgc cgccgtcgga ccggccgctt ccggcccggc ccgggcacgc
      901 tcgtgatggc ccgtggctac ccgcttaccg ccggtgacga ggtcgaggcc gcggttgaag
      961 tggaggcgct ggtcgcgatt ctggccgagc agggcgaaat gagccgcgag gaactggcca
     1021 ggaaggcggg cgccaggcaa tggggtcccg gccggttcgg ctacgtgctc gcccaggctg
     1081 tggccggcgg ccgcgtggtg cgcagcggcc gccgggtgag cctgccctcc ggctgaacta
     1141 cttgagggtc cggatcagct tcttgttgat gaattcctgg atgccgtagg tgcccagctc
     1201 gcggccgaaa ccggaacgct tgacgccacc gaagggaagt tcggcgccgt cgaggccgac
     1261 cccgttcaca tagaccatgc cggtgtcgag gttctcggct acccgccggg cctgttccgg
     1321 atcgtcggtg aagacgtacg aacccagccc gaacggggtg tcattggcga gttcgaccgc
     1381 ttcgtcttca ctcgccacct tgtagacggt ggccaccggc ccgaagaact cttcccggta
     1441 ggcgtcgttc tcgggggtga ctccggtcag cacggtgacc g
//
LOCUS       DAOQFI010000605         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_61797_length_1477_cov_3.372714, whole genome shotgun sequence.
ACCESSION   DAOQFI010000605 DAOQFI010000000
VERSION     DAOQFI010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61797_length_1477_cov_3.372714"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..952
                     /locus_tag="PLE93_09715"
     CDS             341..952
                     /locus_tag="PLE93_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH87369.1"
                     /translation="MSEDRRRQKERTAATRAAVLEATVELLVQRGYAGTSTRLAAEQA
                     GVSLGALQHHFPTKAELTVESMRFVTDRLADEFVSAIPRDGDELERISLALDRLLVVF
                     RGPTFAAALELHLAARTDESLREPIRRLHLEDQNRFRESAIALMPGVEDQPGFDAWLL
                     MSVSAIRGMAVATMGVAEVEDEWQLLKAQLLDSATELLRQESA"
     gene            complement(1001..>1477)
                     /locus_tag="PLE93_09720"
     CDS             complement(1001..>1477)
                     /locus_tag="PLE93_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNH87370.1"
                     /translation="VKAVNWLRERGVMIFAILFGLSMDYNVFLLSRIHEAYNEGDRPR
                     ESVIHGLSRIGKVILFAGLIMASVFSAFVTQDDVVAKMMGIGLGLAILIDVLIVRLVI
                     APAVVTLLGDKAWWLPGWLDRILPNVSLEGHLVENRDPKGEPLAPELANDPIYEKP"
BASE COUNT          255 a          491 c          504 g          227 t
ORIGIN      
        1 gacgcgatcc gcatgaggat ccgcggcgct cgcccgacgg catcgatcag gcgaccacct
       61 tcctccagaa cggaaccatc gccagctgcg aggtgatcac cccgctgccg tccggcgacg
      121 cccactgcta cgcgaacggc tacaccggcc cgaccccctg atcggagggg ccgggccggc
      181 cgccgggccc cggccgtcgc cgaacccgcc cttccggggg cgttaggcca ccatttcggt
      241 ggtcaaacgc ccccggaagc gggggagacg cggggatctg aacttacaag tcggttgacc
      301 gacagatgta tattgcgctc atgagaccgg cgaggacggc gtgagcgagg acaggcgccg
      361 gcagaaggag aggacggcgg caaccagggc cgcggtgctg gaggccacgg tcgagctgct
      421 ggtccagcgt ggctacgcgg gaacttccac gaggctggcg gccgagcagg ccggggtgtc
      481 actgggtgcg ctgcagcacc acttcccgac caaggccgaa ctcacggtgg agtcgatgcg
      541 attcgtcacc gaccggctgg ccgatgagtt cgtctcggcc ataccccgtg acggtgacga
      601 gctcgaacgc atttcgctcg ctctcgaccg tctcctcgtg gtcttccggg gcccgacctt
      661 cgccgccgcg ctggaacttc acctggcggc ccgcacggac gagtccctgc gggagccgat
      721 ccggcggctt catctcgagg atcagaaccg cttccgggag agtgcgatcg ctctcatgcc
      781 cggagtcgag gatcagcccg gtttcgatgc ctggctcctg atgtcggttt cggcgattcg
      841 cggcatggcg gtcgcgacca tgggcgtcgc cgaggtcgaa gacgaatggc agctgctcaa
      901 agcgcagctg ctcgactcgg caaccgaact gctgcggcag gaatccgcct gacccggccg
      961 ggaagccggt gcctccatgc ccgccttggc aactgccggg tcagggcttc tcgtagatcg
     1021 ggtcgttggc cagctccgga gcgagcggct cgcccttcgg gtcacggttc tcgaccaggt
     1081 ggccctcgag gctgacgttt ggcaggatcc ggtccagcca gccgggcagc caccaggcct
     1141 tgtcaccgag cagggtgacc acggccggtg cgatcaccag gcggacgatc aggacgtcga
     1201 tcaggatcgc cagaccgagc ccgatgccca tcatcttggc gaccacgtca tcctgggtca
     1261 cgaaggcgga gaagaccgag gccatgatca ggccggcgaa gaggatcacc ttgccgatcc
     1321 gcgagaggcc gtggatcacg ctttcgcgag ggcggtcacc ctcgttgtag gcctcgtgga
     1381 tgcgggacag aaggaagacg ttgtagtcca tcgagagccc gaagaggatc gcgaagatca
     1441 tcacgccgcg ctcgcggagc cagttgaccg ccttcac
//
LOCUS       DAOQFI010000606         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_61967_length_1475_cov_2.895775, whole genome shotgun sequence.
ACCESSION   DAOQFI010000606 DAOQFI010000000
VERSION     DAOQFI010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61967_length_1475_cov_2.895775"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLE93_09725"
     CDS             <1..355
                     /locus_tag="PLE93_09725"
                     /inference="COORDINATES: protein motif:HMM:NF036901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87371.1"
                     /translation="VPDGSVTAGTGTATQDVIQALARPYPVAVTGTPTSLKFDPDTRV
                     MDLTYGTAKRGGGKFPAPLPSVVSVPRYTYPTGYKAIVKGARVTSAACSPKLRLQTRP
                     GARKVSLTVKPASSC"
     gene            complement(398..802)
                     /locus_tag="PLE93_09730"
     CDS             complement(398..802)
                     /locus_tag="PLE93_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNH87372.1"
                     /translation="MIVADTSVWVDYLNGVWAPGSRELRRLLEEDETVCMTDHVLSEI
                     LQGYRREAEADEIASLLLTFPFLGLEGPGDYLAAASVYRKARSAGITLRSTVDLLIAI
                     PCIRREAFLLHNDRDFDQLARVSDLKIWQPAG"
     gene            complement(799..1008)
                     /locus_tag="PLE93_09735"
     CDS             complement(799..1008)
                     /locus_tag="PLE93_09735"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNH87373.1"
                     /translation="MRTNIVIDDELMAEAQKLTGTKTKKETVDLALREMVAHRRRQGI
                     LELPGKFKWEGDLNEMRRNFFRKFD"
     gene            complement(1101..>1475)
                     /locus_tag="PLE93_09740"
     CDS             complement(1101..>1475)
                     /locus_tag="PLE93_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87374.1"
                     /translation="RIGENVGAIPIQTFPRRYGVTDIYGYPGGAMRTRVMRTCRSHTK
                     ADWYGGRFFYGPTGLLARCNMAAGSSGGPWVSRYKAEGGGTIGVVDGLTSTGFSQGGR
                     SYLTSPYFGRILVRLIKATEGR"
BASE COUNT          269 a          491 c          452 g          263 t
ORIGIN      
        1 ggtacccgac ggatcggtaa ccgcaggcac cggcaccgcc acccaggacg taatccaggc
       61 gctggcccgc ccctatccag tggccgtcac cggcacgccc acctcactga agttcgatcc
      121 ggacacccgg gtgatggacc tgacctacgg cacggcaaag cgtggagggg gcaaattccc
      181 cgccccgctt cccagcgttg tctcggtacc ccgctacacc tacccgaccg ggtacaaggc
      241 catcgtcaag ggggccaggg tcacctccgc tgcctgctcc ccgaagttga gactgcagac
      301 caggcccggc gcccgaaagg tcagcctcac ggtgaagccc gccagctcct gctgacggca
      361 aagctcaacg ggctacctat cccggtgccg gcccgggtca gccggccggc tgccagatct
      421 tcaggtccga gaccctggca agttggtcga agtcgcgatc gttgtgcagg aggaaggctt
      481 cacgacgaat gcaaggaatg gcgatcagga gatcgaccgt gctgcggagg gttattcccg
      541 cagaccgggc ctttcgatag acgctcgctg cggccaggta atcgcctgga ccctccaggc
      601 ccagaaaggg gaaggtgagc aagaggcttg cgatttcatc cgcttccgcc tccctgcggt
      661 agccctgaag aatctcagag agaacgtgat cggtcatgca cacggtttcg tcctcctcga
      721 ggagccgacg gagctctctc gaaccgggtg cccagacacc gttcagatag tcgacccaga
      781 cgctcgtgtc agcgacaatc aatcgaactt ccggaagaaa ttccgacgca tctcgttcag
      841 gtcgccttcc cacttgaact tgcccggcaa ctccaggatc ccttgccggc ggcggtgggc
      901 caccatttcc cgcagggcga gatcgactgt ttccttcttg gtctttgtcc cggtcagctt
      961 ctgggcttcg gccatcagtt cgtcgtcgat gacgatgttg gtcctcatac acatcacgat
     1021 acacatcgcc tgaccctttc tcaacggatc gctccattga ggaggtaagt gccgggggcc
     1081 ggggattcgc cccccggggg tcagcggcct tcggtggctt tgatcaggcg gaccaggatc
     1141 cggccgaagt aggggctggt caagtaggac ctgccgccct gggagaagcc ggtgctggtc
     1201 agaccgtcga ccactcctat cgtgccgcct ccttcggcct tgtaacggga aacccagggg
     1261 ccgccgctgg agccggccgc catgttgcag cgggccagaa ggccggtcgg tccgtagaag
     1321 aaccggcctc cgtaccagtc cgccttggtg tgggaacggc aagtacgcat cacccgggtc
     1381 cgcatcgccc cgcccgggta cccgtagatg tcggtgaccc cgtatcggcg gggaaaggtt
     1441 tggatcggga tcgccccgac gttctctccg atccg
//
LOCUS       DAOQFI010000607         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_62296_length_1471_cov_2.922316, whole genome shotgun sequence.
ACCESSION   DAOQFI010000607 DAOQFI010000000
VERSION     DAOQFI010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62296_length_1471_cov_2.922316"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLE93_09745"
     CDS             <1..668
                     /locus_tag="PLE93_09745"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNH87375.1"
                     /translation="IGTATNRHGCRAKICRFTAPNTTWTTAPNAISPGVAFNGVGGLS
                     GNGAISAFWDELPAGESINGGDCGKPNAVVRDPGAVWLSSGIAEVPAPPTCQELGKDG
                     LWPDCVDIDWPDPDPVVEITKVTVTKGTVTAGQKAKIKVNVTNSGDLAATPITIKLAS
                     SSKQVKVPGQVKLNVPAGKTATASITVTATKNARGKATITASTSGKSGKAVVTVKKPR
                     KRK"
     gene            complement(767..1228)
                     /locus_tag="PLE93_09750"
     CDS             complement(767..1228)
                     /locus_tag="PLE93_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH87376.1"
                     /translation="MIEIRPVAPGEFPLFEPLFVLYQEFYEVKDIDRGRNREFFSRFL
                     VGEDGVGSADGWLLGAFDGEEAVGFGCFYRHKSSLSATDVVLMNDLFVTEAARGGGTG
                     RALIEAGVELARESGASHLTWATAPDNHTAQRLYDSTGAHKSTWLEYEIEA"
BASE COUNT          289 a          471 c          487 g          224 t
ORIGIN      
        1 cgatcggcac cgcgacgaac cgccacgggt gccgcgcgaa gatctgccgg ttcaccgcgc
       61 cgaacacgac ctggaccacc gccccgaacg cgatcagccc gggagtcgcc ttcaacgggg
      121 tcggcggact ttccggcaac ggtgcgatca gcgccttctg ggacgaattg ccggccggcg
      181 agtcgatcaa cggtggcgac tgcggcaagc ccaacgccgt cgtgcgtgac ccgggtgcgg
      241 tctggctttc gagcggcatc gccgaagtcc cggctccccc gacctgccag gaactgggca
      301 aggacgggct ctggcctgac tgcgtcgaca tcgattggcc cgatcccgat ccggtggtcg
      361 agatcaccaa ggtgaccgtg accaagggaa ctgtcacagc cggccagaag gcaaagatca
      421 aggtcaatgt caccaactcc ggggatctcg ccgcgacccc gatcaccatc aagctcgctt
      481 cgagctcgaa gcaggtcaag gtgcccggtc aggtcaagct gaacgtcccg gccggcaaga
      541 ccgcgaccgc gtcgatcacc gtgaccgcga ccaaaaatgc ccggggcaag gcgacgatca
      601 ccgccagtac ctcagggaag tccggcaagg ccgtggtgac cgtaaagaag ccgaggaagc
      661 gcaagtaacg cgagcgcttc acccgacgaa agcgcggagt gagttgcctt tcttcacgag
      721 ctacggagaa ggcgactcgc tccgcgtctt ttgcgttcgg ccgggctcag gcttcgatct
      781 cgtattccag ccaggtggac ttgtgggctc cggtgctgtc gtagagacgc tgggcggtgt
      841 ggttgtccgg ggcagtggcc caggtcaggt gtgaggcgcc ggactcgcgg gcgagctcga
      901 ccccggcctc gatcagggcc cggccggtcc ccccgccgcg cgcggcctcg gtcacgaaaa
      961 ggtcgttcat cagcacgacg tcggtagccg aaagcgagct cttgtgacgg taaaagcagc
     1021 cgaagccgac cgcttcctcg ccgtcgaagg caccgagcag ccagccatcg gcggaaccga
     1081 cgccgtcttc gcccacgagg aagcgggaga agaactcccg gttcctgccc cggtcgatgt
     1141 ccttgacctc gtagaactcc tgatagagca cgaagagcgg ttcgaagagc ggaaattccc
     1201 caggagcaac tggtcggatc tcgatcatgg gatcagcgta ccggcatgat cagagccctt
     1261 ccggcttccg gcgccggccg gagaagatgc aggtgttctt gccggggaag ggagcagggt
     1321 atggtcggag tagaaggtgt ccaacgtcgc ctgcgaccgc agttgagagg ccgcggcggc
     1381 gcttcttcta gagtgggacc aagcgagccc gctcacgacc ccggagggta gatggcaggt
     1441 ccgaccgata ccaacgagca acttctctgc a
//
LOCUS       DAOQFI010000608         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_62436_length_1469_cov_3.371287, whole genome shotgun sequence.
ACCESSION   DAOQFI010000608 DAOQFI010000000
VERSION     DAOQFI010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62436_length_1469_cov_3.371287"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLE93_09755"
     CDS             complement(<1..900)
                     /locus_tag="PLE93_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNH87377.1"
                     /translation="MSSNSKDSFSSKDTLETGGRSYEIFRLDALQKKFDVARLPYSIK
                     VLLENVLRLEDGVSVSAADVEAIASWDAKAEPSDEIPFQPARVLMQDFTGVPAVVDLA
                     AMRDAMADIGGEVSAINPLVDVDLVIDHSVQVDAFGNGMAFEINAEKEFERNLERYEF
                     LKWGQGSFNNFRVVPPATGICHQVNLEYIAQVVYSRENDGVLQAYPDTLVGTDSHTTM
                     VNGLGVLGWGVGGIEAEAAMLGQPVSMLLPQVIGFKLHGKLPAAATATDLVLTVTEML
                     RERGVVGKFVEFYGPGISSLPLTD"
     gene            complement(932..>1469)
                     /locus_tag="PLE93_09760"
     CDS             complement(932..>1469)
                     /locus_tag="PLE93_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH87378.1"
                     /translation="TGRITDLTDEQVARMLRINLESPILLAQALLPGMLERGRGKLVM
                     IGSLAGKAGSPRSSIYNATKFGLRGFAFGLAADLAGSPVSITLVAPGFVREAGMFADS
                     GADAPPGLGTTTPERVAEAVLKAADSKKIEIAVAPLVQRTMAHIGLISPHVSHRVQSG
                     PAAHKAADSLVAGQADKR"
BASE COUNT          238 a          466 c          513 g          252 t
ORIGIN      
        1 gtcggtcagc ggcagcgagc tgatgcccgg gccgtagaac tcgacgaact tgccgaccac
       61 cccgcgctcg cggagcatct cggtcacggt caggaccagg tcggtggcgg tcgcggcggc
      121 cggcagcttg ccgtgaagct tgaagccgat tacctgcggc agcagcatcg aaacgggctg
      181 gccgagcatc gccgcctcgg cttcgatgcc gccgaccccc cagcccagca cgccaaggcc
      241 gttgaccatg gtggtgtgcg agtcggtgcc gaccagggtg tccggatagg cctgaaggac
      301 accgtcgttc tcgcggctgt agacgacctg ggcgatgtat tcgaggttga cctgatggca
      361 gatgccggtc gccggcggga ccacgcggaa gttgttgaag gagccctggc cccacttgag
      421 gaactcgtaa cgctcgaggt tccgctcgaa ctccttctcg gcgttgatct cgaaggccat
      481 gccgttgccg aaggcatcga cctggaccga gtggtcgatc accaggtcga cgtcaaccag
      541 cgggttgatc gcggaaacct cgccgccgat gtcggccatc gcgtcacgca tcgcggccag
      601 gtcgaccacg gccggcaccc cggtgaagtc ctgcatcagg acccgggcgg gctggaaggg
      661 aatctcgtcc gagggctcgg ccttcgcatc ccacgaggcg atcgcctcga cgtcggcggc
      721 gctgaccgag acaccatcct caagccgcag cacgttctcg agcagaacct tgatcgagta
      781 gggcaggcgg gcgacgtcga acttcttctg cagggcgtcg agacggaaga tctcgtagct
      841 cctgccgccc gtttcgagtg tgtccttgct tgaaaacgag tctttggaat tgctgctcat
      901 ggttctcttc tctgtcttga gtgggtgagg gtcaacgttt gtcggcctgg ccggcgacca
      961 gcgagtcggc ggccttgtgg gcggccgggc ccgactggac ccggtgggag acgtgcgggc
     1021 tgatcagccc gatgtgggcc atggtccgct ggaccagtgg cgcgacggcg atctcgatct
     1081 tcttcgagtc ggcggcctta agcactgctt cggccaccct ttcgggggtg gtcgtgccga
     1141 ggccgggcgg ggcgtcggct ccggagtccg cgaacatgcc cgcctcgcgg acgaagccgg
     1201 gagcgaccag ggtgatgctg accggggaac cggccaggtc cgcggcgagc ccgaaggcga
     1261 agccgcgcaa accgaacttg gtcgcgttat agatggagga gcgggggctg cccgccttgc
     1321 cggccagcga gccgatcatc accagcttgc cccggccccg ttccagcatc cccggcaaca
     1381 gagcctgggc gagcaggatc ggtgattcga ggttgatccg cagcatgcgg gcaacctgct
     1441 cgtcggtcag gtcggtgatc cgaccggtg
//
LOCUS       DAOQFI010000609         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_62639_length_1467_cov_2.900850, whole genome shotgun sequence.
ACCESSION   DAOQFI010000609 DAOQFI010000000
VERSION     DAOQFI010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62639_length_1467_cov_2.900850"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLE93_09765"
     CDS             complement(<1..294)
                     /locus_tag="PLE93_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNH87379.1"
                     /translation="MSQTLILVKPDAFERALTGEVLARFERKGLRIAALKKLTATEQI
                     ANEHYAEHKEKPFFGELVEFITGGPLVAAVLEGPEAVVAARQLIGATNPVEAAP"
     gene            complement(386..1156)
                     /locus_tag="PLE93_09770"
     CDS             complement(386..1156)
                     /locus_tag="PLE93_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH87380.1"
                     /translation="MPIAIFGIGNTALNLAVTLAILMLVAIWIALIVFTFTDARRRIA
                     DPFLVGCATAASFFPFVGTLVYTILRPPEFLEDIRERETDITESEVRLRHLKHSSCHK
                     CGSPVESDFIRCPACRSRLKEPCPTCSKPVGLNWKVCPYCETTLIAPKRSSRSSGSKR
                     PKGESSGRPSSSRSGRSERAPEGKEERRRRSSKAPAESGARESRSSSGESRPEDSSRR
                     SGNSSSDQADSSRSPRSARRKISVSDDEKSEPINRGDS"
BASE COUNT          256 a          437 c          524 g          250 t
ORIGIN      
        1 gggcgcggct tcaaccgggt tggtggcgcc gatcagctgg cgggcggcga cgaccgcttc
       61 cgggccttcg aggacggcgg cgaccagcgg gccgccggtg atgaactcga ccagttcacc
      121 gaagaagggc ttctccttgt gctcggcgta atgctcgttg gcgatctgct cggtcgcggt
      181 cagcttcttg agggcggcga tacgaaggcc cttgcgctcg aagcgggcga gaacctcacc
      241 ggtgagggcg cgctcgaagg cgtcgggctt gaccaggatc agggtctggg acatgtcttg
      301 ttactccgtt gtgagtttgg ttgtggccga agccagcggg tttcgtttgg cgtgagccgg
      361 cgggtcagcg tacccgcgca ggggttcagc tgtcgccgcg gttgatcggt tccgacttct
      421 cgtcgtcgct gacgctgatc ttgcgccggg cggaacgagg cgaacgggac gaatcggcct
      481 ggtcggaaga agagttgccc gaacgccggg aagaatcctc ggggcggctt tcgccggacg
      541 aagaccggct ctcgcgggca cccgactcgg ccggggcctt gctggagcgg cgacggcgtt
      601 cttccttccc ttcgggagcc ctctcggagc ggccagagcg ggaagatgat ggacgacccg
      661 aagattcgcc cttggggcgc ttcgagccgg agctgcgcga tgaccgcttg ggagcgatca
      721 gggtggtctc gcagtagggg cagaccttcc agttcaggcc gaccggcttc gagcaggtcg
      781 ggcacggctc cttcagccgg gaccggcagg ccgggcagcg gatgaagtca ctctcgaccg
      841 gggagccgca cttgtggcag gagctgtgct tgaggtgccg cagccgcacc tcggactcgg
      901 tgatgtcggt ttcgcgctca cggatgtcct cgaggaactc cggcggacga aggatcgtgt
      961 agacgagcgt gccgacgaag gggaagaacg aagcggcggt ggcgcagccg accaggaagg
     1021 ggtcggcgat ccggcgacgg gcgtcggtga aggtgaagac gatgagggcg atccagatcg
     1081 cgaccagcat caggatggcc agagtgacgg ccaggttcag cgcggtattt ccaattccga
     1141 agatggcgat aggcatgtga ctagaggaac atccttccga ataggtaccc aatgccgaag
     1201 aagacgagaa tgacgatcgc cacgaccgca gcgaccagcg ccatcatcga cagcagttgg
     1261 gagccagtct gacgattcac cggctaatcg tcgcataacc ggcggctgcc gaaccctccg
     1321 ccccggcttc tgagagacct tgcccgtcgt tcagccctgg aagccggcac cttcgcccgg
     1381 tgcgagaagg aagtgcgaac cggtgatgat caccgctccg cccttctcta aagccatctc
     1441 cctggccctt tccagcgccc cggccgg
//
LOCUS       DAOQFI010000610         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_62796_length_1465_cov_2.936879, whole genome shotgun sequence.
ACCESSION   DAOQFI010000610 DAOQFI010000000
VERSION     DAOQFI010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62796_length_1465_cov_2.936879"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLE93_09775"
     CDS             <1..956
                     /locus_tag="PLE93_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH87381.1"
                     /translation="GADLQLVQRDDESDPATSADEMKELIEQVQAIAVLGPTFSNSAA
                     EADPIANQNKTPVLAVSNTGPGIVGDCDYPCEYIFRDSLGEATAIPANVKNLVDREHP
                     KFAKVLYPVDDPFGQTSAETAVQAFKDEGVKVTSMQVGANGIGQHMTDERQDAFMITA
                     SSGETGAGIVKDLRNAYGAKTPILGGNAFNSPVVAESLGQQGKGVQSAAAWYAGNHSD
                     ENHEFMANYMAKYGQEPDQFAAQAYTGVKLLADAAEEADLQFGDIAADREALKTALEG
                     VSEETPLGDFSFTPDHDVSQPIWIVQMNGKGTYDLVAQVNP"
     gene            complement(1160..>1465)
                     /gene="rpsG"
                     /locus_tag="PLE93_09780"
     CDS             complement(1160..>1465)
                     /gene="rpsG"
                     /locus_tag="PLE93_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNH87382.1"
                     /translation="EDAVETLEEAIKQLTPALEVRSRRVGGATYQVPVEVPSRRARTL
                     AVRWLVDFARARNEYSMSERLANEILDATKQQGNAYKRKDDIFRMAQANKAFAHYRW"
BASE COUNT          294 a          471 c          452 g          248 t
ORIGIN      
        1 acggggccga ccttcagctg gtccagcgcg atgacgaatc cgacccggcc acctcggccg
       61 acgagatgaa ggaactgatc gagcaggttc aggcgatagc cgtgctcggc ccgaccttct
      121 ccaactctgc cgccgaagcc gatccgatcg ccaaccagaa caagactccg gtcctcgccg
      181 tctccaacac cggccccgga atcgtcggtg actgcgacta cccctgcgag tacatcttcc
      241 gcgattccct cggcgaggcc accgcgatcc cggccaacgt caagaacctg gtggaccggg
      301 agcacccgaa gttcgccaag gtgctctacc ccgtggacga ccccttcggt cagaccagcg
      361 ccgagaccgc agttcaggcg ttcaaggacg aaggtgtcaa ggtgacatcg atgcaggtcg
      421 gtgccaacgg catcggtcag cacatgaccg acgaacgcca ggatgccttc atgatcactg
      481 cctcttccgg agagacgggg gccgggatcg tcaaggatct tcgtaacgcc tacggcgcca
      541 agactccgat cctcggcggc aatgccttca actcgccggt cgtcgccgag agcctcggcc
      601 aacagggcaa gggcgtccag tccgctgccg cctggtacgc gggcaatcac tcggatgaga
      661 accatgagtt catggcgaat tacatggcga agtacgggca ggaaccggac cagttcgccg
      721 cccaggccta caccggggtc aagttgctgg ccgacgcggc ggaggaagcc gatctccagt
      781 tcggtgacat cgccgccgac cgcgaagccc tcaagaccgc cctggaaggg gtcagcgaag
      841 aaaccccgct cggtgacttc agcttcaccc ccgaccacga cgtaagccag ccgatctgga
      901 tcgtccagat gaacggcaag ggcacctacg acctggtcgc acaggtcaat ccctaaccga
      961 actccgaccc ctcgacacga agtgggtatc tatttcgtct atatagacga aatccatagt
     1021 cacctcgctc ctggcgagcg ggaaatccga ggtgggtatg agattcgttc atatggacga
     1081 aatccatagt cacctcactg gtctgcaatg aaaagggcgg ctcggtggcc gcccctttcg
     1141 caagtcttga tttcgcctgc taccagcggt agtgggcgaa ggccttgttg gcctgggcca
     1201 tgcggaagat gtcgtccttc cgcttgtagg cgttgccttg ctgcttggtc gcgtcgagga
     1261 tctcgttggc gaggcgctcg gacatcgagt actcgttgcg ggcgcgggcg aaatcgacca
     1321 gccagcggac cgcgagggtg cgggcgcgcc gggagggaac ctcgaccggg acctggtagg
     1381 tggcgccacc gacgcggcgg gagcgaacct caagggccgg ggtgagctgc ttgattgcct
     1441 cttcgagagt ctcgacggca tcctc
//
LOCUS       DAOQFI010000611         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_62888_length_1464_cov_2.784954, whole genome shotgun sequence.
ACCESSION   DAOQFI010000611 DAOQFI010000000
VERSION     DAOQFI010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62888_length_1464_cov_2.784954"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PLE93_09785"
     CDS             complement(<1..1174)
                     /locus_tag="PLE93_09785"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNH87383.1"
                     /translation="MRYPVESFTPEEQASLAPYFTNLDQPVFALTNLPETVKGALFAR
                     YSRYGGTLRRLYLDEFAADAAAADPEGRLRPFDGEEGQRAAKLYESVFVGFGDDSIAQ
                     LGGAHVACEWVSNILTKVLQRGRLAAYLEQSTRYIAYDQEMPGGGYRYLSGDALDGQF
                     EAAMNDIFLIYSDSLTRVEAWAAERWPRGDDEPEGPWRRSIKAKALDLLRGLLPASTL
                     SHVGIYASGQAWEQLLFRMMASPLPEARRFGGMILDELNKVIPSFVSRVSRPDRGGEW
                     ISFLEQRRQATEDWVERLGLTDRDSEDSGPTVELGHVHGTEDDLLAACLYEESDAPES
                     RIRERIARLDSRQREELMAAMIGERRNRRHRPGRGFESVSYRFEIVSDYGAFRDLQR"
BASE COUNT          254 a          485 c          496 g          229 t
ORIGIN      
        1 ggcgctgaag atcccggaag gcgccgtagt cggagacgat ctcgaagcgg tacgagaccg
       61 actcgaaccc gcgaccgggg cggtggcgac ggttgcggcg ctcgccgatc atcgccgcca
      121 tgagttcttc gcgctggcgg gaatcaagcc gggcgatccg ttcccgaatc cgcgattcgg
      181 gagcgtcgga ctcctcgtaa aggcaggccg cgagcaggtc gtcttcggtg ccgtgaacat
      241 ggccaagctc aaccgtcggt ccgctgtctt cgctgtcgcg gtctgtgagg ccgagccttt
      301 cgacccagtc ttcggtcgcc tggcggcgct gctcgagaaa ggaaatccac tcgccgccgc
      361 gatcgggacg gcttacccgg gaaacgaaac tggggatcac cttgttcagc tcgtcgagaa
      421 tcatcccgcc gaagcgacgg gcctccggta acggcgatgc catcatccgg aacagcagct
      481 gctcccaggc ctggcccgag gcgtagatcc cgacatgact gagagtcgaa gccggcaaaa
      541 ggccccgcag caggtccagt gccttggcct tgatcgaacg gcgccagggg ccttcgggct
      601 catcgtcacc gcgcggccag cgctcggcgg cccaggcttc cacccgggtg agtgaatccg
      661 agtagatcag gaagatgtcg ttcatcgccg cttcgaactg gccgtcgaga gcgtcgccgc
      721 tcaggtagcg gtagccgcca ccgggcatct cctggtcgta ggcgatgtag cgggtggact
      781 gctcgaggta ggcagccagc cgaccgcgct gcagcacctt ggtcaggatg ttcgacaccc
      841 attcgcaggc gacgtgggca ccaccgagct gggcgatcga atcgtcaccg aaaccaacga
      901 agacgctctc gtagagcttc gccgcacgct ggccttcctc cccgtcgaag ggcctgaggc
      961 gcccttccgg gtcggcggcc gcggcgtcgg ctgcgaactc gtcgagatag agccttcgca
     1021 gggtcccccc gtaacgggaa tagcgggcga agagggctcc cttgaccgtt tcgggcaggt
     1081 tcgtgagcgc gaacacgggc tggtcaaggt tggtgaaata gggagcgagc gaggcctgct
     1141 cctccggagt aaagctctcg accgggtagc gcacccgtcg aacctaacag gcgcgcccga
     1201 cgctcgactg ccgggttatg tagacccgga cccgatcgcg aagtccggcg tcgccgggcc
     1261 agccgcacgc ggccccgaag gggcgggttc agtcctcggg aacccggcgc agagacccgc
     1321 ccgccccggc cgccaggacg ttgatccggt tgtgcccccg gcgcatcacc gccggcctga
     1381 ccatgaagcc gaaccggacc ttgccctggt aggtgaagct gcgggtggtg gcgacgatcg
     1441 tcccgttcca ggccagggcc atca
//
LOCUS       DAOQFI010000612         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_63271_length_1459_cov_3.239316, whole genome shotgun sequence.
ACCESSION   DAOQFI010000612 DAOQFI010000000
VERSION     DAOQFI010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63271_length_1459_cov_3.239316"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLE93_09790"
     CDS             <1..630
                     /locus_tag="PLE93_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87384.1"
                     /translation="AFIPPYAKSPPTPERKSSGFLRLAVRRARVYRLSLLFLSIYGVP
                     MILGAWMIEYLSSEGDVSKTIAGLASFLLFGLSAVMRFGGAELRAMGWTHKVLCGSLG
                     LAAAGLAAIAIDPVAGAAFLAAVLLATGFGLPYATALDEAQDLYPEEPAEPLALMTLV
                     ALLLPIVVIPLVGHAISNDAGDIAFGALALFLVVATLANLKRSGIPLTR"
     gene            658..>1459
                     /locus_tag="PLE93_09795"
     CDS             658..>1459
                     /locus_tag="PLE93_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87385.1"
                     /translation="MEPSASSEVAAKKIEPGTPGDGLILNPKSWSAPHLDAADQERLK
                     SMIQWFENRGLKALKDDDRENRWYSDFLEMQAELGIFCNFLTPARDGEGDPARRWDTT
                     RICEFNELSGFYGLPYWYTWQVSILGLGPIFQSENEAARKKAADELARGSIFAFGLSE
                     RAHGADIYSTDMVLTPAGDDGAGETYTATGGKYYIGNGNEAAMVSVFGRVDGIEGPDG
                     YAFFAASSSHSNYELVRNVVNSSNYVSQFNLNAYPVESADLLHQGPGAF"
BASE COUNT          232 a          514 c          452 g          261 t
ORIGIN      
        1 gcgttcatcc cgccttacgc gaagagcccg ccgaccccgg aaaggaagtc ctccggcttt
       61 ctccggctgg cggtcaggcg cgcccgggtc taccggctgt ccctgctctt cctttccatc
      121 tacggggtgc cgatgatcct cggcgcctgg atgatcgagt acctctccag tgagggggac
      181 gtttccaaga cgatcgccgg cctcgcttca tttctgctgt tcggcctctc ggcggtgatg
      241 cgcttcggcg gggcggagct gagggcgatg ggctggaccc acaaggtgct ttgcggctcg
      301 ctcggcctgg ccgcagccgg cctcgccgcg atcgcgatcg atcccgtcgc cggggccgcc
      361 ttcctcgccg cggtgctgct cgcaaccggg ttcggcctgc cctacgccac cgcgctcgac
      421 gaggcccagg acctctatcc cgaggagccg gccgagccgc tcgccctgat gaccctggtc
      481 gccctgctct tgccgatcgt ggtcatcccg ctggtcggcc acgcgatctc gaatgacgcc
      541 ggtgacatcg ccttcggcgc gctcgccctg ttcctcgtcg tggcgacgct cgccaatctg
      601 aagcgatccg gcatccctct gaccaggtga accctgatcc gggcgtatgc tcccggcatg
      661 gagcccagcg ccagttctga agttgccgcc aagaagatcg agcccggaac ccccggtgac
      721 ggactgatcc tcaacccgaa gagctggtcg gcgccacatc tggatgccgc cgaccaggag
      781 cggctcaagt cgatgatcca gtggttcgag aaccgtggcc tcaaagccct gaaggacgac
      841 gaccgcgaga accgctggta ctcggacttc ctcgaaatgc aggccgagct ggggatcttc
      901 tgcaacttcc tcactccggc ccgggacggt gaaggcgacc cggccaggcg ctgggacacg
      961 acccggatct gcgaattcaa cgagctgtcc ggtttctacg ggctgcccta ctggtacacc
     1021 tggcaggtct cgatcctcgg cctcggtccg atcttccagt ccgaaaacga ggccgcgagg
     1081 aagaaggcgg ccgacgagct ggccaggggc tcgatcttcg ccttcggcct ctccgagcgg
     1141 gcccacggtg ccgacatcta ttcgaccgac atggtcctta ccccggccgg tgacgatggc
     1201 gcaggggaga cctacaccgc gaccggcggc aagtactaca tcggcaacgg caacgaagcg
     1261 gcgatggtct cggtcttcgg tcgggtcgac gggatcgaag gcccggacgg ttacgccttc
     1321 ttcgcggcca gctccagcca ctcgaactac gaactggtcc gcaacgtggt caattcatcc
     1381 aactacgtct cccagttcaa cctgaacgcc tatccggtcg agtcggccga cctgcttcac
     1441 cagggcccgg gcgccttcg
//
LOCUS       DAOQFI010000613         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_63882_length_1452_cov_4.065140, whole genome shotgun sequence.
ACCESSION   DAOQFI010000613 DAOQFI010000000
VERSION     DAOQFI010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63882_length_1452_cov_4.065140"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLE93_09800"
     CDS             complement(<1..388)
                     /locus_tag="PLE93_09800"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNH87386.1"
                     /translation="MSDHGLLEMDAVREVFENSIDFTIGLEEEFAIVDPDSLELVDRY
                     PDLYAAAQDDPVLAGSAAGELIASEIEIRSGRSETMSEATKRQEEKRRHLFRLAEAHS
                     VALASLGTHPWADYLDQEIIDTPHYQR"
     gene            complement(390..1058)
                     /locus_tag="PLE93_09805"
     CDS             complement(390..1058)
                     /locus_tag="PLE93_09805"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNH87387.1"
                     /translation="MSQTPKSRTDNPLLVGEGERLALGVAARLSRYLQVLIQARKMGR
                     ETISSQELSEYTHINPTQIRRDLSGFGKFGKRGVGYRIDYLIDEIRKIMRTAGQHNIA
                     LFGAGKLGQAIAGSDVFADHGFRIVSLFDVDPEMIGKEVGQLTVRDFAELGEAVQEEA
                     IVVGVLAVPSAAAQEVADGLVEAGVTVIFNYTEQLLQVPPKVTVHTSSPAVDLLYALY
                     FYLS"
     gene            complement(1080..>1452)
                     /locus_tag="PLE93_09810"
     CDS             complement(1080..>1452)
                     /locus_tag="PLE93_09810"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNH87388.1"
                     /translation="QVQPAARLSGLPLFRRFLDRPPGQSLTGAGRTFEVTFLSRPGCH
                     LCDHALAELNEYLAGRGGDGQAVPGVIVEVINIDTDDELHRRFLERIPVILVGERIVS
                     EFSFEPEALESVLGDWSQGAA"
BASE COUNT          277 a          447 c          472 g          256 t
ORIGIN      
        1 accgctggta atggggggtg tcgatgatct cctggtcgag atagtcggcc caggggtggg
       61 tcccgagtga agccagggcg accgagtggg cttcggccag ccggaagaga tggcggcgct
      121 tctcctcctg ccgtttggtc gcctcgctca tcgtttcgga ccggcccgag cggatttcga
      181 tctccgaggc gatcagctcc ccggcggcgg acccggccag caccgggtca tcctgggccg
      241 cggcgtagag gtccggatag cgatcgacca gttccaggct gtccggatcg acgatcgcga
      301 actcttcctc gaggccgatc gtgaaatcaa tcgagttctc gaagacctcc cgcacagcat
      361 ccatttccag caggccgtgg tcggacaaat caggagagat agaagtagag ggcgtaaagc
      421 aggtcgaccg ccgggcttga ggtatggacc gtgaccttgg gcggcacctg aaggagttgc
      481 tcggtgtagt tgaagatcac ggtcacgcct gcttcgacca ggccgtctgc gacctcctgg
      541 gcggcagccg aaggcacggc cagcaccccg accacgatcg cctcctcctg gaccgcctca
      601 ccgagctcgg cgaaatcgcg aaccgtgagc tggccgacct ctttgccgat catttccgga
      661 tcaacgtcga aaagggaaac gatgcggaag ccgtgatcgg cgaacacgtc cgaaccggcg
      721 atcgcctgac cgagcttgcc cgcaccgaac agcgcgatgt tgtgctggcc ggccgtccgc
      781 atgatcttgc ggatctcgtc aatcaggtag tcgatccggt accccacccc gcgtttgccg
      841 aatttcccga agccggaaag gtcgcggcgg atctgggtcg ggttgatgtg ggtgtactcg
      901 gagagttcct gcgaggagat ggtctcgcga cccatcttcc gggcctggat cagcacctgc
      961 aggtagcgcg aaagccgtgc cgcgaccccg agagcgaggc gctccccttc gccaaccagc
     1021 agcggattgt cggtgcggga tttgggagtt tgtgacatcg ggctacaagc gtacccgtct
     1081 caggcagcgc cctgtgacca gtcgcccagg actgactcca aagcttcagg ttcgaagctg
     1141 aattcagaga cgatgcgctc gcccaccagg atcaccggga tccgttccag aaaccggcga
     1201 tggagctcat cgtcggtatc gatgttgatc acttcgacaa tcaccccggg aacagcttgc
     1261 ccatccccgc cgcgtccggc gagatactcg ttcagctcgg ccagggcgtg atcacaaagg
     1321 tggcagccgg gacggctcag gaaggtgact tcgaaagtcc ggcccgctcc ggtcaacgat
     1381 tgccccgggg gccggtcgag aaagcgtcga aagagaggta gtccggatag gcgagcggct
     1441 ggctgaacct gg
//
LOCUS       DAOQFI010000614         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_63884_length_1452_cov_3.869721, whole genome shotgun sequence.
ACCESSION   DAOQFI010000614 DAOQFI010000000
VERSION     DAOQFI010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63884_length_1452_cov_3.869721"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLE93_09815"
     CDS             complement(<1..599)
                     /locus_tag="PLE93_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87389.1"
                     /translation="MNSLAQRLPTTGADRSLARQMLNKVPEVTLYFWIIKVLCTTVGE
                     TAADYMNVDLGFGLTNTTYAIGALLVVALIFQFRSRRYVPGIYWTAVVLISVVGTLIT
                     DNLVENMNVALQTTTIGFSVALAITFVVWYAFERTLSIHSITTTRREAFYWLAILFTF
                     ALGTSAGDQIAEQYGLGYFVSILIFAGKIGSSIGAELGS"
     gene            complement(645..718)
                     /locus_tag="PLE93_09820"
     tRNA            complement(645..718)
                     /locus_tag="PLE93_09820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(682..684),aa:Thr,seq:tgt)
     gene            complement(813..1406)
                     /gene="sigH"
                     /locus_tag="PLE93_09825"
     CDS             complement(813..1406)
                     /gene="sigH"
                     /locus_tag="PLE93_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HNH87390.1"
                     /translation="MIVLAKQGDPTAYDRLVRRYYGFVRLKASSYFLAGGDADDLIQE
                     GLVGLYKAIRDYRTDRESSFRNFAELCITRQIITAVKTATRNKHVPLNQYVSFGQSPA
                     SNTESETTMEEILPGPTALDPVNQAISSEELSSLVSCLQSTLSEMESSVLGYYLEDRS
                     YEDVAALLGCETKTVDNALQRVKRKVSKHLESRDIAV"
BASE COUNT          288 a          408 c          486 g          270 t
ORIGIN      
        1 atcgatccca gctcggcgcc gatgctggaa ccgatttttc cggcaaaaat caggatggaa
       61 acgaaataac cgagcccgta ctgctcggcg atctggtcgc cggccgaggt tcccagcgcg
      121 aaggtgaaca gaattgcgag ccagtagaag gcctctctgc gggtggtcgt aatcgagtgg
      181 atcgagaggg tccgctcaaa ggcgtaccac acgacaaagg tgatcgccaa cgccaccgag
      241 aacccgatcg tcgtggtttg gagcgcgaca ttcatgttct ccacgaggtt gtcggtgatc
      301 aacgtgccaa ccacgctgat caggaccacg gcagtccagt agatccccgg cacgtagcgt
      361 ctggatctga actggaagat cagggctacg acgagcaagg cgccgatcgc ataggtggta
      421 ttggtcaacc cgaagccaag gtcgacgttc atgtaatcgg ccgcggtttc tccgaccgtg
      481 gtgcagagaa ccttgatgat ccagaagtag agggttacct ccggaacctt gttgagcatc
      541 tggcgggcca gagaccggtc cgccccggtt gtcgggagtc tttgagcaag gctgttcatg
      601 ccggaagaat ccactcccga acctgaacga ctcctgacag cgagagcccg gctccgggct
      661 cgaaccggaa acctcctgtt tacaagacag gtgctctagc caattgagct agccgggcag
      721 gaggcgtgat tctgacagcc ccggcaggct ttggccccgg ccgggtgcgg tccggcgcgg
      781 ggcgatggcc tgtcaggccg aatgccgctt gttcagacgg cgatgtcgcg tgattcgagg
      841 tgcttgctga ccttgcgctt gacccgctgg agggcgttgt cgacggtctt ggtctcgcaa
      901 ccgagcaggg cggcgacgtc ctcgtaggag cggtcctcga ggtagtagcc gagcacgctg
      961 ctttccattt ccgagagggt cgactggagg caggaaacga gggaggagag ttcttcggaa
     1021 gaaatcgcct ggttgaccgg gtcgagcgcg gtcggccccg ggaggatctc ctccatcgtg
     1081 gtctccgact cggtgttcga ggccggcgac tggccgaagg acacgtactg gttgagcggg
     1141 acgtgcttgt tgcgggtcgc ggtcttcacc gcggtgatga tttgccgggt gatgcagagc
     1201 tcggcgaagt tgcgaaaact cgactcgcgg tcggtccggt aatcccgaat cgccttgtag
     1261 aggccgacca ggccctcctg gatcaggtcg tcggcgtcgc ccccggccag gaagtacgag
     1321 gacgccttga gccggacgaa tccgtagtag cggcggacga gacgatcgta ggcggtggga
     1381 tcaccctgct tggcaaggac gatcaggaag ccgtcatcga gacgtacgtc agcctcgtgc
     1441 tcaagccgag aa
//
LOCUS       DAOQFI010000615         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_63915_length_1452_cov_2.418039, whole genome shotgun sequence.
ACCESSION   DAOQFI010000615 DAOQFI010000000
VERSION     DAOQFI010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63915_length_1452_cov_2.418039"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLE93_09830"
     CDS             <1..775
                     /locus_tag="PLE93_09830"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNH87391.1"
                     /translation="FARAYRGHPFIYVPDVITRLSRRRVLVTEFVEGIGFEEILELGD
                     EQRGRFGEILFRGSFGSIYHLQHFNADPHPGNYLLMKDGRVAFLDFGMTKKLDAEQIE
                     LEQLAVDAAVRNDPEGLRDALHNLGFVKKPSKLDAEHLMNHVMSIGGWYMEDREIEID
                     GDRVMKVIESTHDPRSEYFDLMRRESIPADELMGRRMEIGVLAVLGRLRAKRNWHRIM
                     REWVYADPPATELGREEWEYFEERGIFQVPGIKKGKLES"
     gene            772..>1452
                     /locus_tag="PLE93_09835"
     CDS             772..>1452
                     /locus_tag="PLE93_09835"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HNH87392.1"
                     /translation="MKRIKAGWQLTKKSWRVLSDSPGLVRFPLIGGLIALLITIVLIG
                     PGLYLLEDGNSAPGAILAAVGIYLAAFATFYFAVGLAHNADRQMQGETATFGDGMALA
                     SSRMGAIAGWAFVATVVMTIIRAIQERFGIAGAIVGGLAGAAWGILTFLAVPVIALEG
                     TGPIETIKRCASLVKSRWGEQIAGTIAIGGIVFLVGFLPATLLIVAGVALWTVSGIGG
                     AILIAVGVL"
BASE COUNT          241 a          452 c          485 g          274 t
ORIGIN      
        1 cttcgcccgc gcctaccgtg gccatccgtt catctacgtc cccgatgtga tcacccgtct
       61 ttcccggcgc cgggtgctgg tcaccgagtt cgtcgagggg atcggcttcg aggagatcct
      121 cgaactcggt gacgagcagc gcggccgttt cggcgagatc ctcttccgcg gcagcttcgg
      181 ctcgatctac cacctgcagc acttcaacgc cgaccctcac cccggcaatt acctgttgat
      241 gaaggacggc cgggttgcct tcctcgattt cggcatgacc aagaagcttg acgcggagca
      301 gatcgagctc gaacagctgg cggtcgacgc ggcggttcga aacgatccgg aaggccttcg
      361 cgacgcgctc cacaacctgg gcttcgtcaa gaagccgtcg aagctggatg ccgagcacct
      421 gatgaaccac gtgatgtcga tcggtggctg gtacatggaa gaccgcgaga tcgagatcga
      481 cggcgaccgg gtgatgaagg tgatcgagtc aacccacgat ccccgctccg agtacttcga
      541 cctgatgcga cgggaatcga tcccggccga cgagttgatg ggccgccgga tggagatcgg
      601 tgtgctggcc gtgctcggcc ggctgcgggc gaagcgcaac tggcaccgga tcatgcgcga
      661 gtgggtgtac gccgacccgc cggcgaccga gctgggcagg gaggaatggg agtacttcga
      721 agagagaggg atttttcagg tccccgggat caagaagggt aaattggagt catgaagcgc
      781 atcaaagctg gttggcagtt gaccaagaaa agctggcgcg ttctttcgga ctcgcccggc
      841 ctggtgcggt tcccgctgat cggcggcctg atcgcactcc tgatcacgat cgtgctgatc
      901 ggccccgggc tctacctgct cgaggacgga aacagcgctc ccggggcgat tctcgcggcg
      961 gttggcatct accttgcggc ctttgccacc ttctacttcg cggtcgggct cgcccacaac
     1021 gccgaccggc agatgcaggg ggaaaccgcc acttttggcg acggcatggc tttggcctcg
     1081 agccggatgg gagcgatcgc cggctgggcc ttcgtggcga ccgtcgtgat gacgatcatc
     1141 cgggcgattc aggagcgctt cggaatcgcc ggcgcaatcg tcggtggtct ggccggcgcc
     1201 gcctggggca tcctcacttt cctcgccgtc ccggtcatcg ctctcgaagg caccgggccg
     1261 atcgagacga tcaagcgttg cgccagtctg gtcaagagcc gctggggcga gcagatcgcc
     1321 ggcaccatcg cgatcggcgg cattgtcttt ctggtcggct tcctgccggc cacgctgctg
     1381 atcgtcgccg gggtagcgct ctggaccgtg tcgggtatcg gcggcgcgat cctgatcgcc
     1441 gtcggggtgc tc
//
LOCUS       DAOQFI010000616         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_64052_length_1450_cov_2.549104, whole genome shotgun sequence.
ACCESSION   DAOQFI010000616 DAOQFI010000000
VERSION     DAOQFI010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64052_length_1450_cov_2.549104"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1065)
                     /locus_tag="PLE93_09840"
     CDS             complement(121..1065)
                     /locus_tag="PLE93_09840"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNH87393.1"
                     /translation="MEKAEYIWHSGKFVPWDEAQVHVLSHGLHYGTGVFEGVRCYDGE
                     QGPAIFRHKEHLQRFEDSARMYFLDLPFTKEEIGEATRELIRKNGLRDCYIRPLAFRG
                     YGEMGLYAKSAPVEVIIAVWPWGAYLGEDGKKHGIRAKVSSWRRVSSASLIPHAKASG
                     QYLNSILAKTESAKAGYEEAILLDERGMVCEGSGENIFMVKEGKVFTPPQVASILDGI
                     SRKSVMQILEDKGYPVIERDIARSELYLADEIFMCGTAAEVVPVREVDDHPLGEPGEV
                     TRLVQQGYEDAIYGRSPEYSEWLDLVGEPAAKTEPSTV"
     gene            complement(1133..>1450)
                     /locus_tag="PLE93_09845"
     CDS             complement(1133..>1450)
                     /locus_tag="PLE93_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNH87394.1"
                     /translation="GSLGMLPSASIGAEGQPGLFEPVHGSAPDIAGQGVANPLATMLS
                     AAMMLRYGLGDGAGADRIDDAVDQVLAKGLRTPDIFDGGEGETRVGTEEVTAAVLAEL
                     GQG"
BASE COUNT          240 a          480 c          442 g          288 t
ORIGIN      
        1 accttgatcg ggtccgggag gactgcacac ggcatcccgc cgcgctgccg cggggtcagc
       61 gccccgcagc ccccgtaatt cgggtgatcc tgatttccct gatggcgtga agcatccgtc
      121 tcagactgta ctgggttcgg tcttcgccgc cggttcgccg accaggtcga gccattccga
      181 gtattcgggc gagcggccgt agatcgcgtc ctcgtatccc tgctggacca gccgggtgac
      241 ttcgcccggc tcgccgagcg gatggtcgtc gacctcgcgg accggaacca cttcggcggc
      301 ggtgccgcac atgaagatct cgtcggccag gtagagctcc gaacgggcga tgtcccgctc
      361 gatgaccggg tagcccttgt cctcgaggat ctgcatgacc gacttgcggc taatcccgtc
      421 gagaatcgaa gcaacctgtg gcggtgtgaa gactttgcct tccttgacca tgaagatgtt
      481 ttcgccggag ccttcacaga ccattccccg ttcgtcaagc aggatcgctt cttcgtaacc
      541 ggctttggct gattcggtct tggccaggat cgagttcagg tactgcccgg aagccttcgc
      601 atgcgggatc aagctggccg aggaaacacg gcgccaggaa gaaaccttgg cccggatgcc
      661 gtgtttcttg ccgtcctcgc cgaggtatgc cccccagggc cagaccgcga tgatgacctc
      721 gacgggagct gactttgcgt agagacccat ctcgccgtag ccgcgaaaag ccagtgggcg
      781 gatatagcag tcacgcaacc cgttcttgcg gatcagctcg cgcgtcgcct cgcctatttc
      841 ttccttggtg aaaggcaggt cgaggaagta catgcgggcc gaatcctcga agcgctgaag
      901 atgctctttg tggcggaaga tcgccgggcc ctgctcgccg tcgtagcaac gaacgccctc
      961 gaacaccccg gttccgtagt ggagaccgtg gctcagtaca tgcacctgcg cctcgtccca
     1021 ggggacgaat ttgccgctgt gccagatgta ctcagccttt tccactgctt ctccttcggg
     1081 ttgcttatcc cagtgatggt acgccagttc cctccggcgt actccaagaa gtctagccct
     1141 gacccagctc ggccagaacc gctgccgtga cttcttcggt gccgacgcgg gtttccccct
     1201 cgccaccgtc aaagatgtcc ggcgtcctca ggccctttgc gaggacctgg tcgaccgcgt
     1261 cgtcgatccg gtctgccccg gcgccgtcgc cgaggccgta gcgcagcatc atcgcggcgg
     1321 aaagcatcgt cgcgagcggg tttgccaccc cttggccggc gatgtccggg gccgatccgt
     1381 gaaccggttc aaagagcccc ggctggcctt cggccccgat cgaggccgag ggcagcatgc
     1441 ccagcgaacc
//
LOCUS       DAOQFI010000617         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_64492_length_1445_cov_2.918705, whole genome shotgun sequence.
ACCESSION   DAOQFI010000617 DAOQFI010000000
VERSION     DAOQFI010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64492_length_1445_cov_2.918705"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PLE93_09850"
     CDS             <1..923
                     /locus_tag="PLE93_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH87395.1"
                     /translation="TRPVSDRLAATLEEFEPVELDIRDAEGVNRVFADRQVELVVHTA
                     AQPSHDWAARDPQTDFGVNALGTLNLLEAARAHAPGAPFIFCSTNKVYGDTPNFLPLI
                     ETDTRLELPEDHEYFDGIDTGMSIDHSTHSLFGVSKASADLLVQEYGRYFEMPTVCFR
                     GGCLTGPQHAGAELHGFLAYLMKCVVTGRPYTVFGYDGKQVRDNIHCDDLVSAFLEFA
                     AAPRAAAVYNIGGGRGSNCSMLEAITESEQIAGKELDWTMSPENRIGDHRWWISDLGA
                     FERDYPNWQRRFGITEILEQIHEYAVEAAA"
     gene            complement(944..>1445)
                     /locus_tag="PLE93_09855"
     CDS             complement(944..>1445)
                     /locus_tag="PLE93_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87396.1"
                     /translation="TSTSTYLGMVAYAGEANDPELVLNLGPGSEVPIDRFLDQVKTSG
                     SPGYSAGEIQSRDAGVRAAADQDLIELTKPLLSGFNPGGNFDRCREYPPDVTVAVRSG
                     VRVIEIRSLGDQILNLGLARFGDPPGYPIGSTHAGSMGWLEIPAIDDPPPWQVTGDGP
                     FAVCYR"
BASE COUNT          246 a          491 c          477 g          231 t
ORIGIN      
        1 ccacccgtcc ggtcagcgac cggctcgccg cgaccctcga ggagttcgag ccggtcgagc
       61 tcgacatccg tgacgccgaa ggggtgaacc gggtcttcgc cgaccgtcag gtcgaactgg
      121 tcgtccacac cgcggcccag ccttcgcatg actgggcggc gcgggacccc cagaccgact
      181 tcggggtaaa cgcgctcggc accctgaacc tgctcgaggc ggcccgggca cacgcgccgg
      241 gtgccccctt catcttctgc tcgaccaaca aggtctacgg tgacacgccg aacttcctgc
      301 cgctgatcga aaccgacacc cggcttgaac tgcccgaaga ccacgagtac ttcgatggca
      361 tcgacaccgg catgtcgatc gaccattcga cccattcgct tttcggggtt tcaaaggcca
      421 gcgccgacct gctggtgcag gagtacggcc gctacttcga gatgccgacg gtctgcttcc
      481 ggggcggttg cctgaccggc ccccagcacg ccggggccga actccacggc ttcctcgcct
      541 acctcatgaa gtgcgtggtc accggccggc cctacacggt cttcggctat gacggcaagc
      601 aggtccgcga caacatccac tgtgacgacc tggtctcggc cttcctcgag ttcgcggccg
      661 cgccgcgggc agccgcggtc tacaacatcg gcggcggccg gggcagcaac tgctcgatgc
      721 tggaagcgat caccgagtcg gagcagatcg ccggcaagga gctcgactgg accatgagcc
      781 cggaaaaccg gatcggcgat caccgctggt ggatttccga cctcggtgcc ttcgagcgtg
      841 actacccgaa ctggcagcgc cgcttcggga tcaccgagat cctcgagcag atccacgagt
      901 acgcggtcga agccgccgcc tgaagcccgg ccggttcgcc cggtcagcgg taacagacgg
      961 cgaaggggcc gtcaccggtg acctgccatg gcggcggatc atcgatggcg gggatttcga
     1021 gccagcccat cgatccggcg tgggtggagc cgatcgggta gccgggaggg tcgccgaagc
     1081 gggcgagccc gaggttgagg atctggtccc caaggctcct gatctcgatc acccgcacgc
     1141 cggacctgac cgcaacggtg acatcgggcg ggtactcgcg gcatcggtca aagttgccgc
     1201 cggggttgaa gccggaaagc agcggtttgg tcagctcgat caggtcctga tcggcggcgg
     1261 ccctcacccc ggcatcccgg gactggatct cgccggccga atacccgggt gaaccgctgg
     1321 tcttgacctg atcgaggaag cggtcgatcg ggacctcgga ccccggtccg aggttgagta
     1381 cgagctccgg gtcattcgct tccccggcgt aggcgaccat gccgaggtag gtgctggtcg
     1441 aggtc
//
LOCUS       DAOQFI010000618         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_64599_length_1444_cov_2.185025, whole genome shotgun sequence.
ACCESSION   DAOQFI010000618 DAOQFI010000000
VERSION     DAOQFI010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64599_length_1444_cov_2.185025"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLE93_09860"
     CDS             <1..576
                     /locus_tag="PLE93_09860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNH87397.1"
                     /translation="TLLDLLDEHDAKATFFLIGKWSEREPELIREVVARGHAIGNHTF
                     THPTMPAKSDERIREELDACREAVEAAGVTFTEIAGRKLMRPPYGRRRPGTLRVLKEK
                     GYLGITWSITGYDWRESITTEKITRNCLKAGEGDIILLHDGFDREPAYDRHKSIETTR
                     NLLEHYRPQGFDFVTVPQLVEAAGTGSRYAA"
     gene            576..1298
                     /locus_tag="PLE93_09865"
     CDS             576..1298
                     /locus_tag="PLE93_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87398.1"
                     /translation="MFEDLNITLTVLLFLWCFGLGAIEIEIEGGYGWAERLPTWYLKR
                     GRTGRVYGWFMGHRPLTGYHVYAFTIPLVILHLPFGMGVEWSLPAELATIAKYFLLAV
                     IWDYCWFVLNPAYTVKRFKRGNVWWFEVPWIWRFPLDYYFGVLLSIGLVALASWDAGS
                     GEILREHLWLVAGLFVLTGLTALFAPLYHRWYRHMRRAGADDREITPKYGPPAPEEVW
                     SGGIPDLAPLEGDDGRQRESDA"
BASE COUNT          241 a          500 c          466 g          237 t
ORIGIN      
        1 accctgctcg acctgctcga cgagcacgac gccaaggcga ccttcttcct gatcggaaag
       61 tggtccgaac gggagcccga gctgatccgc gaggtggtcg cccgcggcca cgcgatcggc
      121 aaccacacct tcacccaccc gaccatgccg gccaaatccg acgagcggat ccgggaagaa
      181 ctggatgcct gccgcgaggc ggtcgaggcg gccggggtga ccttcaccga gatcgccggg
      241 cgcaagctga tgcgaccgcc ctacggccgg cggcggcccg gcaccctgcg ggtcctcaag
      301 gagaagggtt acctcgggat cacctggtcg atcaccggtt acgactggcg ggaaagcatc
      361 accaccgaga agatcacccg caactgcctc aaggcgggcg agggcgacat catcctgctc
      421 cacgacggct tcgaccggga accggcctac gaccgtcaca agtcgatcga gacgacccgg
      481 aacctgctcg agcactaccg cccgcagggc ttcgacttcg tgaccgtgcc gcagctggtc
      541 gaagcagccg gcaccgggtc ccgttacgcg gcctgatgtt cgaagacctc aacatcacgc
      601 tcaccgtcct gctcttcctc tggtgcttcg gcctcggggc catcgagatc gagatcgaag
      661 gcggctacgg atgggccgag cggctcccga cctggtacct gaagcgcggc cggaccggca
      721 gggtctacgg ctggttcatg ggccaccggc cactgaccgg ctaccacgtc tatgcgttca
      781 cgattccgct ggtcatcctc cacctgccct tcggcatggg agtggaatgg tcgctgccgg
      841 ccgagctggc gacgatcgca aagtacttcc tgctggcggt gatctgggac tactgctggt
      901 ttgtcctcaa cccggcctac acggtgaagc ggttcaagcg gggcaacgtc tggtggttcg
      961 aggttccctg gatctggcgc ttcccgctcg attactactt cggggtgctg ctctcgatcg
     1021 gcctggtcgc gctggcctca tgggatgccg gttcgggcga gatcctccgc gagcacctct
     1081 ggctggtcgc cggcctcttc gtcctgaccg gcctgaccgc cctcttcgcc ccgctctatc
     1141 accgctggta ccggcacatg cgccgggccg gcgccgacga ccgggagatc accccgaagt
     1201 acggcccacc ggccccggaa gaagtctggt ccggcggcat ccccgacctg gccccgctcg
     1261 agggcgatga tggtcggcag cgcgagtccg acgcctgagg cgaatcccgt cgcggcggcg
     1321 gcacccgaca gcgttgaagt tattcgcaca tggagcgaat aacttcaacg ctgtcgggag
     1381 cggatgcgcg ggtcagttga cgagcaggcc gccgtcgacc ggtagcaggg ccccggtcac
     1441 gtac
//
LOCUS       DAOQFI010000619         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_64704_length_1442_cov_3.179524, whole genome shotgun sequence.
ACCESSION   DAOQFI010000619 DAOQFI010000000
VERSION     DAOQFI010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64704_length_1442_cov_3.179524"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..>1442)
                     /locus_tag="PLE93_09870"
     CDS             complement(143..>1442)
                     /locus_tag="PLE93_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNH87399.1"
                     /translation="DGVDTSGINARLDVGGTISGSVTFEPGFPVPGICVYADKPGAAG
                     YWSGFARVSPDGHYRIKGLETGDYKLEFRGCGDSDNVARQFYPGKATYSEAVPVSVTQ
                     GSETPGIDADLAPGGAVTGTVFPAESPAEVCAMSVKAYDSDGNLAGSFNSPGFLYTPL
                     DYRIDQLITGNYRLHFEQGCLAIFSMDPFWVSEYFNNRNSLVEATPVSVTEGSTTSQI
                     SAVLGSESTISGTITDTEGAPLENICVEAADENGPAGIIAYSDPDGHYSLNALLGGSY
                     TLKFSDCQGTNERIVTPEYFDDKATIEEAVPVSVSAGGLRSGINARLATAPRPVPTAK
                     ISKLKVKGPAFVRKGREATFRLRITNSGDATATGVRIKGSGRGISARAPVGKIAASTT
                     RTVRVRLRPSKAGKTMVTFRVTSSNAGGRTTKHKINVRKK"
BASE COUNT          243 a          452 c          444 g          303 t
ORIGIN      
        1 tcctgatcgc acttggccga accggtcacg atcgcgtttc cgttgccggt tgcattcgtg
       61 ccggtttcgg agtcgtcatc gctgccgcac ccgaacagca tcgcggcaca ggcaatcgtc
      121 aaagttgctg ccagaacgga tttcactttt tcctcacgtt gatcttgtgt tttgtggtcc
      181 tgccgccggc gttcgatgaa gtgacccgga aggtgaccat cgtcttgccg gccttgctcg
      241 gtctcagcct gaccctgacg gttctggtcg tcgacgcggc gatctttccg accggcgccc
      301 tggcgcttat gccgcgtccg ctgcccttga tccggacccc ggtcgcggtg gcgtcaccgg
      361 agttggtgat ccgcaacctg aatgtcgctt cccggccctt cctgacgaaa gcgggaccct
      421 tcaccttcag cttgctgatt ttcgccgtgg gcactggtct cggcgcggtt gccagccggg
      481 cgttgatgcc cgatctcaac ccgccggcgg agaccgatac cggcacggct tcctcaatcg
      541 tggccttgtc gtcgaagtac tcgggggtca cgatccgctc attcgtgccc tggcagtcac
      601 tgaacttcaa tgtgtatgaa ccgccgagca gcgcgttcaa cgagtaatgg ccgtccggat
      661 cgctgtaagc gatgatcccg gccggtccgt tttcatcggc ggcctcgacg cagatgttct
      721 cgagcggagc accttcggtg tcggtgatcg tgcccgagat cgtgctttcc gatcccagta
      781 cagcgctgat ttgggacgtg gtcgaacctt cggtcaccga aaccggtgtg gcttcgacga
      841 gggaattccg gttgttgaag tactcggaaa cccagaacgg atccatcgag aagatcgcga
      901 ggcaaccctg ttcgaaatgg agcctgtagt tcccggttat gagctggtcg atcctgtagt
      961 caaggggcgt gtaaagaaaa cccggggagt tgaacgagcc ggcaagattc ccgtcggagt
     1021 cgtaggcctt gaccgacatt gcgcaaacct cggccggtga ctcggcggga aacacggtgc
     1081 cggtgacagc gcctcccggc gccaggtcgg cgtcaatccc cggggtctca gacccctggg
     1141 ttaccgatac cgggacggct tccgagtagg tcgccttgcc gggatagaac tgccgggcca
     1201 cgttgtcgga gtcaccgcat ccccggaatt cgagcttgta atcgccggtt tccaacccct
     1261 tgatccggta gtggccgtca ggggaaacgc gggcgaatcc cgaccagtag ccggcggcgc
     1321 ctggcttgtc cgcgtacacg cagattcccg ggacggggaa acccggctcg aaggtcacgg
     1381 aacccgagat cgtcccgccc acgtcgagcc gggcgtttat gcccgaagtg tcaaccccgt
     1441 cc
//
LOCUS       DAOQFI010000620         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_64709_length_1442_cov_3.043259, whole genome shotgun sequence.
ACCESSION   DAOQFI010000620 DAOQFI010000000
VERSION     DAOQFI010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64709_length_1442_cov_3.043259"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLE93_09875"
     CDS             complement(<1..1157)
                     /locus_tag="PLE93_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH87400.1"
                     /translation="MLDENRLAVLNAVCDTIVPSLERDDDPTGFWARSAADTKANEVV
                     AQTIGEMPVADQEGMGLLLDSLALQNFPSLSQASREQILVNTSLASREAAVGVAALTQ
                     MTLFFHYGLPPNPAWEQFGFPGPASPPPQVEKTIHPLTPGDGDVLEADAVIVGSGAGG
                     GVIAARLAEAGMKVIVLEMGGYFNESDFDQTELNGFARMYWRGGPTYSADFNISIQAG
                     SCLGGGTLINWTNSLKPKPWVRQEWADEYGLEDVNTPDFDRHIDSIWERSKVNHDCSE
                     LNQTQKTWIEAAEKLGWSWHKTDRNWDPEKHDPLVAGYMGWGDQSGAKQSTLKTYLQD
                     AADHGAEIIVGCQAQKVLVENGRAAGVEATIEGVGRITVRSPRVVVAGGAL"
     gene            complement(1257..>1442)
                     /locus_tag="PLE93_09880"
     CDS             complement(1257..>1442)
                     /locus_tag="PLE93_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87401.1"
                     /translation="LVVGPTRTAQFSGPGTARHCFKTLKPTFMTHPASGALEGVGLKV
                     FPHQETTREDDRLFRPA"
BASE COUNT          205 a          478 c          498 g          261 t
ORIGIN      
        1 tcgagcgcgc cgcccgcaac gacgacccgg ggcgagcgga ccgtgatccg gccgacgccc
       61 tcgatcgtcg cttcgacccc ggcggcgcgg ccgttttcga ccagcacctt ctgggcctgg
      121 cagccgacga tgatttcggc gccgtggtcg gcggcatcct gcaggtaggt cttgagggtc
      181 gactgcttgg ccccggactg atcaccccag cccatgtagc cggcaaccag cgggtcgtgc
      241 ttttccgggt cccagttgcg gtcggtcttg tgccagctcc agccgagctt ttcggccgct
      301 tcgatccagg tcttctgggt ctggttcagc tccgagcagt cgtgatttac cttcgaacgc
      361 tcccagatcg aatcgatgtg gcggtcgaag tcgggggtgt tcacgtcctc caggccgtac
      421 tcgtcggccc attcctgccg gacccagggc ttcggcttga ggctgttggt ccagttgatc
      481 agggtgccgc cgccgaggca gcttccggcc tggatgctga tgttgaaatc ggcggagtag
      541 gtcgggccgc cgcgccagta catccgcgcg aacccgttca gctcggtctg gtcgaagtcg
      601 gactcgttga agtacccgcc catctcgagc acgatcacct tcatgccggc ctcggccagt
      661 cgggcggcga tcacaccgcc accggcgccg gagccgacga tcacggcgtc ggcttcgagc
      721 acgtcgccgt cacccggggt gagcgggtgg atcgtctttt ccacctgggg agggggagag
      781 gccgggccgg ggaagccgaa ctgctcccag gccggattcg gcggcaggcc gtagtggaag
      841 aagagggtca tctgggtcag cgccgcgacc ccgaccgccg cttcgcggct ggccagcgag
      901 gtgttgacga ggatctgctc gcgcgaggcc tgcgagagcg aagggaagtt ctgcagcgcc
      961 aggctgtcga gcaagagacc catgccctcc tggtctgcca cgggcatctc gccgatcgtc
     1021 tgcgcgacga cctcgtttgc tttggtgtcg gcggcggacc gggcccagaa cccggtcggg
     1081 tcatcatccc gctcaagcga cggcacgatc gtgtcgcaga cggcattcag gacggcaagg
     1141 cggttctcgt caagcactct cgtgctcctc tctcctcggt tcctctccgg ccggatcagt
     1201 ctagcgccgg aaagccggcg ccccgctcat ccgatggggg tgattcgccg cgcgggtcag
     1261 gccggccgga agagccggtc gtcctcgcgg gtcgtctcct gatggggaaa caccttgagg
     1321 ccgacacctt cgagggcgcc gctcgccgga tgggtcatga aggtcggctt cagcgtcttg
     1381 aagcagtgcc tggcggtgcc ggggccgctg aactgcgcgg tccgggtcgg cccgacgacc
     1441 ag
//
LOCUS       DAOQFI010000621         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_64976_length_1439_cov_3.112717, whole genome shotgun sequence.
ACCESSION   DAOQFI010000621 DAOQFI010000000
VERSION     DAOQFI010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64976_length_1439_cov_3.112717"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLE93_09885"
     CDS             complement(<1..446)
                     /locus_tag="PLE93_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH87402.1"
                     /translation="MTTQKVTADMTITLDGFASGRGQSRERPFGDLDHKRLHHWMFEH
                     REDNLAEAEAIVDAGAFIMGRKMFSAEEGNEWDLDWTGWWGSDPPYHAPTFVLTHLPR
                     ASIEMEGGTTFHFVTDGIEAAMEMAREAAGERNVSIAGGPGTLNQYL"
     gene            complement(443..1291)
                     /locus_tag="PLE93_09890"
     CDS             complement(443..1291)
                     /locus_tag="PLE93_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH87403.1"
                     /translation="MFDPDSYREQSRRIWGEMASGWRDRNDWMMRVTGPVNAWLVENL
                     DPQPGQSILEVASGTGELGFLVAERVGSEGRVISTDFSPAMVEVAGRFGEARGASNVE
                     HKVLDAEQMDLDEASVDGVVCRWGYMLMADPAAALKESRRVLRPGGRFAFAVWASPEL
                     NPWAAVPGMTLVQRGHMPPPEPGAPGIFAMADPARINELVTRAGFPEPEIQEIAFTFD
                     YPDSDDVWEAIVRIAGPLAEVINHLDEDEREATRAAILENLAGFRNEDGSYSAPAASW
                     GIVSGR"
BASE COUNT          233 a          507 c          432 g          267 t
ORIGIN      
        1 aggtactggt tcagggtgcc ggggccgccg gcgatcgaga cgttcctctc accggcagcc
       61 tcccgggcca tctccatcgc cgcttcgatt ccgtcggtga cgaagtggaa ggtggtgccg
      121 ccctccattt cgatgctggc ccggggcagg tgagtgagaa cgaaggtcgg cgcgtggtag
      181 ggagggtccg aaccccacca gccggtccag tcgagatccc actcgttgcc ttcctcggcc
      241 gagaacatct tgcggcccat gatgaaagcg ccggcgtcaa cgatcgcttc ggcttcggcg
      301 aggttgtctt cccggtgctc gaacatccag tggtgcagcc gcttgtggtc gaggtcgccg
      361 aagggccgct cccggctctg gccccgcccg ctcgcaaagc cgtccagggt gatcgtcatg
      421 tcggccgtga ctttctgggt ggtcatcggc cggacactat tccccagctc gccgccgggg
      481 ccgaatagga accatcttca ttgcgaaacc ccgccaggtt ttccaggatg gcggcccgtg
      541 tcgcctctcg ctcgtcttcg tcaaggtggt tgatcacctc ggcgagcggc ccggcgattc
      601 gcacgatcgc ctcccagaca tcgtcggaat ccgggtaatc gaaagtgaag gcgatctcct
      661 gaatttccgg ttccggaaac ccggctctgg tgaccagttc attgatccgg gcagggtcgg
      721 ccatggcgaa gattccgggt gcccccggtt cggggggagg catgtggccc cgctggacca
      781 gggtcattcc gggtaccgcc gcccagggat tgagctcggg ggaagcccag accgcgaagg
      841 cgaaccggcc tccgggccgc agcacccttc ggctctcctt caaggccgct gccggatcgg
      901 ccatcagcat gtagccccag cgacagacca cgccgtccac gcttgcttca tccagatcca
      961 tctgctcggc atccagcacc ttgtgctcga cgttcgaggc gccgcgggcc tcaccgaagc
     1021 gcccggccac ctcaaccatc gccggcgaga aatcggtgga aatcacccgc ccctcgctgc
     1081 caaccctttc ggcgaccagg aaaccgagct cgccggtccc cgatgcgacc tcgaggatgc
     1141 tctggccggg ctgcggatcc aggttctcca caagccatgc gttcaccgga ccggttaccc
     1201 gcatcatcca gtcgttccgg tcgcgccagc ccgaggccat ctcgccccag atccgccggc
     1261 tctgctcacg ataggaatcg ggatcaaaca tttcaacctc ctcggttcat ctgcctgccc
     1321 tccctcgaag ctaaccggtc tcagcccggc ccgcttgctt tttttgcggg ccccggctac
     1381 tttgatctca ggcgcactcg caggcaccga tcactcgtgc gtcaaacaga cccgggcac
//
LOCUS       DAOQFI010000622         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_65440_length_1434_cov_2.374909, whole genome shotgun sequence.
ACCESSION   DAOQFI010000622 DAOQFI010000000
VERSION     DAOQFI010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65440_length_1434_cov_2.374909"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="rsmA"
                     /locus_tag="PLE93_09895"
     CDS             <1..798
                     /gene="rsmA"
                     /locus_tag="PLE93_09895"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNH87404.1"
                     /translation="HRQRSPRLRMVKLGQNFLADPKLLDAIVRDAGLSPEDVVLEVGA
                     GEGVLTERLAEVASQVHVIELDRGLEPGLSGVIGRPNVNLVWADAVKYDLAGLDPAPT
                     RMVANLPYAVATPVILRSAFELPSIESWSVMVQKEIGDRLRAGPGSKTYGAPSVLIQL
                     TGEVRQVRKVSRTVFKPQPRVDSAIIRIDRTSPGPDEATRRLVRAGFAHRRKAMARSV
                     DLAIPGSLRAVRDGLEAAGIDPGIRAEALSPAQFAKLSGMIDLAADD"
     gene            791..>1434
                     /locus_tag="PLE93_09900"
     CDS             791..>1434
                     /locus_tag="PLE93_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87405.1"
                     /translation="MTELDLPLELNAPAKLNLCLYLGPRRKDGLHELTSIFEPLTLND
                     TIRVTEGGPAALVDQVICPGVPGENLVSQVIRMMRSEGWDPPLLKVEIEKRIPVQAGL
                     GGGSADAAAMLRLGVGEVEDLAGLALLLGADVPSQLNPKPLLVEGVGQKLTTLPAPAE
                     HWVVLLPFERGLSTRAVFDEADRMKLGRSQEEIDDISGRLWAVATNAVSPLAYPA"
BASE COUNT          226 a          496 c          494 g          218 t
ORIGIN      
        1 caccgccaac gcagcccgcg tctacggatg gtgaagctcg gccagaactt cctggccgac
       61 ccgaagctgc tcgacgcgat cgtccgggat gccggcctct cgcccgaaga cgtggtcctg
      121 gaagtaggtg cgggggaggg agtgctgacc gagcgcctgg ccgaagtcgc ttcgcaggtt
      181 cacgtgatcg agctcgaccg gggcctcgag ccggggctgt ccggcgtgat cggcaggccg
      241 aacgtgaacc tggtctgggc cgacgcggtg aagtacgacc tggccggact cgacccggcc
      301 ccgacccgga tggtcgccaa cctgccctac gccgtggcga ccccggtgat cctccgcagc
      361 gccttcgagc tgccttcgat cgagagctgg agcgtgatgg tccagaagga gatcggcgac
      421 cggctgcggg ccgggccggg cagcaagacc tacggggccc cttcggtcct gatccagctg
      481 accggcgagg tgaggcaggt tcgcaaggtg agccggaccg tcttcaagcc ccaaccccgg
      541 gttgactccg cgatcatccg gatcgaccgc acctctcccg gccccgacga ggcgacccgg
      601 cggctggtcc gggccggctt cgcccaccgc cgcaaggcga tggcccgttc ggtcgacctc
      661 gccatcccgg gcagcctcag ggcggtccgg gacgggctcg aagcggccgg gatcgacccg
      721 ggcatccggg cagaggcgct ttcgcccgcc cagttcgcaa agctttccgg gatgatcgat
      781 ctcgctgccg atgactgaac ttgatctccc cctcgaactg aacgcgccgg ccaagctgaa
      841 cctctgcctt tacctggggc cgaggcgcaa ggacggcctg catgaactca cctcgatctt
      901 cgagccgctg accctcaacg acacgatccg ggtaaccgaa ggaggcccgg cggccctggt
      961 cgaccaggtg atctgcccgg gcgtgccggg cgagaacctg gtcagtcagg tgatcaggat
     1021 gatgcgctcc gagggctggg atccccctct gctcaaggtc gagatcgaaa agcggatccc
     1081 ggtccaggcc gggctcggcg gcggcagcgc cgatgccgcg gcaatgctcc gcctcggggt
     1141 tggagaggtc gaggacctgg ccgggctcgc cctgctgctc ggcgccgatg tgccttcgca
     1201 gctcaatccg aagcccctgc tggtcgaggg ggtcgggcag aaactgacca ccctcccggc
     1261 gccggccgaa cactgggtcg ttctgctgcc tttcgagcga ggactttcga cccgggcggt
     1321 gttcgatgaa gccgaccgga tgaagctcgg ccggagccag gaagagatcg acgacatttc
     1381 cgggcgcctc tgggcggttg ccaccaacgc ggtgtccccg ctcgcctacc cggc
//
LOCUS       DAOQFI010000623         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_65544_length_1433_cov_2.151669, whole genome shotgun sequence.
ACCESSION   DAOQFI010000623 DAOQFI010000000
VERSION     DAOQFI010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65544_length_1433_cov_2.151669"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLE93_09905"
     CDS             complement(<1..415)
                     /locus_tag="PLE93_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HNH87406.1"
                     /translation="MTSSLTERRTSLYETHVEAGAKMVPFAGWEMPVQYDGIKEEHIA
                     VRTHAGIFDVSHMGEIEVEGPGSLAFLQRLVSNDVSKIEIGGAQYSLLLNEDAGVIDD
                     LFVYRLGHDRYLVVTNAGNHQADLQQFGLLARDFDV"
     gene            complement(503..>1433)
                     /gene="ftsZ"
                     /locus_tag="PLE93_09910"
     CDS             complement(503..>1433)
                     /gene="ftsZ"
                     /locus_tag="PLE93_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNH87407.1"
                     /translation="DIKISIGQELTRGLGAGGRPEIGAGAAAETRDEIKEALKGADMV
                     FVTAGEGGGTGTGSAPIIAEIAKEEIGALTVGAVTKPFEFEGKKRMQQAMEGIEKLRN
                     HVDTLIIVPNEKLLQAVERRTSVLDAFRMADDILRQGVQGITDLITIPGLINLDFADV
                     RTIMRDAGSALMGVGAGQGENRAIDAAKAAITSPLIEESIKGATGMLLNITGPEELGL
                     FEVNEAAQLVQSEADPNANIIFGSVVDQSLVDEVRVTVIATGFEGFELLARTPQRVRR
                     RYESRKRVSSEDTDISNLRVGDGDIEVPPFLQD"
BASE COUNT          224 a          459 c          466 g          284 t
ORIGIN      
        1 atacgtcgaa gtccctggcg agcaggccga actgctgaag gtcggcctga tggttaccgg
       61 cattggtgac gaccaggtag cggtcatgac cgagccggta gacgaacagg tcgtcgatca
      121 ccccggcgtc ctcgttcaga agcagcgagt actgcgctcc tccgatctcg atcttcgaca
      181 cgtcattgct gaccagccgc tgcagaaagg cgagtgagcc cggcccttcc acctcgatct
      241 cgcccatgtg ggagacgtcg aagatcccgg cgtgggtgcg cacggcgatg tgttcttcct
      301 tgatcccgtc gtactggacc ggcatctccc agccggcgaa cggcaccatt ttcgcgccgg
      361 cttcgacatg ggtttcgtag aggctggtcc tgcgttcggt gagcgacgaa gtcacggggt
      421 cagcctacgc caaagcaggc accggcgaag cgccggagct catgcagttt ctgccgggtc
      481 cgcgtagccg cggggtcggt cgctagtcct gaaggaaggg cggtacttcg atgtcgccgt
      541 caccgacacg gaggtttgag atgtccgtgt cttccgatga gacccgcttg cgcgactcgt
      601 agcggcgccg gaccctctgc ggggtgcggg ccagcagctc gaagccttcg aagccggtcg
      661 cgatcacggt cacccggacc tcgtcgacca gggactggtc aacgaccgag ccgaagatga
      721 tgttcgcgtt cgggtcggct tccgactgga ccagctgggc cgcttcgttg acctcgaaga
      781 ggccgagctc ctcggggccg gtgatattca ggagcatgcc ggtcgcgccc ttgatcgatt
      841 cctcgatcaa cggcgaggtg atcgcggcct tggcggcgtc gatcgcgcgg ttttcgccct
      901 gaccggctcc gacgcccatc agggccgaac cggcgtcgcg catgatcgta cggacatcgg
      961 cgaagtcgag gttgatcaga cccgggatgg tgatcaggtc ggtgattccc tggacgccct
     1021 gccgcaggat gtcgtcggcc atgcggaatg cgtcgaggac gctggtcctc cgttcgaccg
     1081 cctgcagcag cttctcgttg ggaacgatga tcagcgtgtc gacgtggttg cgaagcttct
     1141 cgatgccttc catcgcctgc tgcatccgct tcttgccttc gaactcgaag ggcttggtga
     1201 cggcgccaac cgtcagtgcg ccaatttctt ccttggcgat ctcggcgatg atcggggccg
     1261 aaccggtgcc cgtgcctcca ccttcgcccg cggtcacgaa gaccatgtcc gcacccttga
     1321 gcgcttcctt gatctcgtcg cgggtttcgg ctgccgcgcc ggcgccgatc tcgggccggc
     1381 cgccggcacc gaggccgcgg gtcagttcct gcccgattga aatcttgatg tcc
//
LOCUS       DAOQFI010000624         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_65700_length_1431_cov_2.536337, whole genome shotgun sequence.
ACCESSION   DAOQFI010000624 DAOQFI010000000
VERSION     DAOQFI010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65700_length_1431_cov_2.536337"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLE93_09915"
     CDS             complement(<1..840)
                     /locus_tag="PLE93_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87408.1"
                     /translation="MRNEESYDNQVQEPSAGRLEALSGHESERSISSIDSGLDAVEPG
                     LVADEAYPRPADAAGSGAEGTESTRMLNPALEVAAGRERNAFSGPDSRVGYVYHRLLA
                     ISDLVAIVLAASIAVFLANTFDRSPDLDGYVAALVVMLPVWLLIAYVGGLFHQVDRRI
                     TQDYVDEIGPVVVAATAWIWLFVILRATFVSGITELTAPILIWILMIASLLFFRTALR
                     IFARRRDWNRRPVATIGDAFGLSAISERMKRHPEWSLEVRSEIEMDLTGNFVLHSSSE
                     SEPE"
BASE COUNT          324 a          445 c          408 g          254 t
ORIGIN      
        1 ctcgggctcg gattcacttg atgaatgcag cacgaagttg ccggttagat ccatctcgat
       61 ttcacttcga acctccaggg accactcggg atggcgcttc attcgttcgg aaattgcgct
      121 cagtccgaat gcgtcgccga tcgtcgcaac cggcctacgg ttccaatcgc gcctccgggc
      181 gaaaatcctg agggcggttc tgaagaacag gagcgaagcg atcatcagga tccaaatcag
      241 tatcggtgcg gtcagctctg tgatgcccga aacgaaagtc gcgcggagaa tcacgaacag
      301 ccagatccac gcggtcgcgg ccaccacaac cggtccgatt tcgtcaacgt agtcctgggt
      361 tatgcggcgg tcaacctgat ggaagagacc accgacatag gcgatgagaa gccagaccgg
      421 aagcatcacc acgagcgccg ccacgtatcc gtcaaggtcc ggacttcggt cgaatgtgtt
      481 cgccaggaac accgctatcg aggcagccag gacgatcgcc accagatccg aaatcgcaag
      541 cagacggtgg tagacatagc caacccggct gtccgggccg ctgaacgcgt tccgctcgcg
      601 acccgctgcg acttccaagg ccggattaag catcctggtg gattccgtac cctcggcacc
      661 ggaaccggcg gcatctgccg gtcgggggta ggcctcatcg gcgaccagac ccggctcgac
      721 cgcgtccaga cctgagtcta tggaagaaat ggacctttcg gactcatgtc cagacaaagc
      781 ttctagcctt ccagcgctgg gttcttgaac ctgattatcg taactctctt cattccgcaa
      841 agttctggtc gaatcgcaaa actggctacg gatgcccggg ggaaaccacc gattttccgg
      901 gtttcgcaag ataccgggat tgatagcctc ccgccgaagt cagcccacct ccgggacccg
      961 gaatcccgat gacctctgaa taccttctat gaccggtccc gaaccagcct gatgcacatt
     1021 caactctgga gttgcaacta cgatccggaa ccgatgggga tcgccccact cagcgcaatt
     1081 tgggcgaagg cgatggccga tcgagggcac gaggtcgaag tcgtggcggc tcacccgcac
     1141 tatccccagc cagattggga ttcaaagctg ctgccttacc gcgaggttcg aaacggtatt
     1201 cctgtgacgc gactgccgct gatcatcgga cgaaagcgga gatctcagcg aatgatccag
     1261 gaaatgagct tcatggcctc ccagacggac gagacgatca acgccgagat cctcggccgg
     1321 aactggatcc accttcggga cggcagcggc agccccgaca agggcgacaa cgacatcacc
     1381 gtgaccacca aggacaacgc cgccgtcggc gaggtggtca ccgtgcgcgg c
//
LOCUS       DAOQFI010000625         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_65832_length_1429_cov_4.221252, whole genome shotgun sequence.
ACCESSION   DAOQFI010000625 DAOQFI010000000
VERSION     DAOQFI010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65832_length_1429_cov_4.221252"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLE93_09920"
     CDS             <1..281
                     /locus_tag="PLE93_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH87409.1"
                     /translation="PDVERAEAERMAAIAKALGDPIRLTLVDVLRKHAGKVCVCELVP
                     LFDVSQSTLSHHLKKLRDAGIVDSERQGLWAYYYVNPEELDELSAWLS"
     gene            269..1147
                     /gene="arsM"
                     /locus_tag="PLE93_09925"
     CDS             269..1147
                     /gene="arsM"
                     /locus_tag="PLE93_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNH87410.1"
                     /translation="MAELEQVNDCCTPEAQASCCEAEARDECCGEEQGCGCQEGSERI
                     ESELREIVRERYAAAAVDAGSRDASCCANDTVITEEQRAVFGSNLYEEGDRSELPEAA
                     QLASLGCGNPTAVADLEEGQTVLDLGSGGGIDVLLSARRVGPTGKAFGLDMTDEMLEL
                     ARSNQKEAGVENVEFLKGEIEAVPLPDDSVDVVISNCVINLSGDKARVLREAARVLRP
                     GGSFAVSDVIADPDMTEETRADMREWTGCIAGALTREEFESNLAAAGFEDIQIEATHR
                     VHDQAESAIIRATLPA"
     gene            1144..>1429
                     /locus_tag="PLE93_09930"
     CDS             1144..>1429
                     /locus_tag="PLE93_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87411.1"
                     /translation="MSTDEIQLNDPQTLYERWEGAQWNPFTVPLEWDIEQWAGMGDRD
                     RGLVYWVLSSLMVAEERITTKFSGLVGAHGSEEEASFLATQQVDEARHIGY"
BASE COUNT          306 a          424 c          476 g          223 t
ORIGIN      
        1 acccggacgt cgaacgcgcc gaagccgaac gaatggccgc gatcgcaaag gcacttggcg
       61 acccgatccg gctcacactg gtcgacgtac ttcggaaaca cgccggcaag gtctgcgtct
      121 gcgagctcgt acccctgttc gacgtcagcc agtcgacgct ctcccatcat ctgaagaagc
      181 tgagggatgc cggaatcgtc gattcagaac gccagggact gtgggcctat tactacgtga
      241 accccgaaga gctggacgag ttgagcgcat ggctgagcta gagcaggtga acgactgttg
      301 caccccggag gcccaggctt cctgctgcga agccgaagcc agggatgagt gttgcggtga
      361 agagcagggt tgcgggtgcc aggaaggaag tgaacgcatc gaatccgaac tcagggagat
      421 cgtgcgagag aggtacgcgg cggcggcagt tgacgccggc tcccgggatg cgtcctgctg
      481 cgcgaatgac acggtcatca ccgaagagca gcgggcagta ttcggatcga acctctacga
      541 agaaggcgac cgaagcgaac tgcccgaagc tgcacagctc gcctcgctcg gctgcggaaa
      601 tccgactgcc gtcgctgatc tcgaagaagg tcagaccgtt ctcgatctcg gatccggtgg
      661 cggcatcgac gtcctgctct cggcccggcg ggtcggcccg accggcaagg ccttcggcct
      721 cgacatgacc gacgagatgc tcgaactcgc tcgcagcaac cagaaggaag ccggtgtcga
      781 gaacgtcgaa ttcctgaagg gggagatcga agcggtaccc ctccccgacg actcggttga
      841 cgtggtgatc tccaactgcg tgatcaacct ttccggcgac aaagccaggg tcctgaggga
      901 ggcggcgcgg gtccttcgac cgggcgggag ttttgccgtc tcggacgtga tcgccgatcc
      961 ggacatgacc gaagagacac gggcggacat gagggaatgg accggctgca tagccggcgc
     1021 cctgacccgc gaagagttcg aatcgaacct tgcggcagcc ggcttcgagg acatccagat
     1081 cgaggcgaca caccgcgtcc acgaccaggc cgaatcggcg atcatccgag ctaccctgcc
     1141 cgcatgagca ccgacgagat ccagctgaac gatccccaga ccctctacga aaggtgggaa
     1201 ggggcgcagt ggaatccgtt cacggtaccg ctggagtggg acatcgagca gtgggccgga
     1261 atgggggaca gggatcgagg actggtttat tgggtgctct cgtcactgat ggtcgccgag
     1321 gagagaatca cgaccaagtt ctccggtctg gtcggggccc acggatcgga agaggaagcg
     1381 agctttctcg cgacccagca ggtcgacgag gcccgccaca tcggctacg
//
LOCUS       DAOQFI010000626         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_66200_length_1425_cov_3.484672, whole genome shotgun sequence.
ACCESSION   DAOQFI010000626 DAOQFI010000000
VERSION     DAOQFI010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66200_length_1425_cov_3.484672"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLE93_09935"
     CDS             complement(<1..483)
                     /locus_tag="PLE93_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87412.1"
                     /translation="MNVNWDGEDLGESLPGRQGRLLFAYLALNRHRPVRRDELVEALW
                     AEEGLPSGGEALLAPPLSRLRKALGPGRLEGRSELELALGDDAWVDWEAAQAGIEEAR
                     LDESAGRHREAYELAGEAEKILSGGLLPGLEAGWIDEYRNELDDLRLQALELIARSGS
                     Q"
     gene            624..>1425
                     /locus_tag="PLE93_09940"
     CDS             624..>1425
                     /locus_tag="PLE93_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNH87413.1"
                     /translation="MAPETPEEVASALVEAGRQSRKVVVFSTGHGIPPIGDTSDRVLL
                     DMSAFDKVEIDPLARTARIGGATQWMELISAANEHGLTAPFGSAATVGVTGYMMGGGV
                     GFMSRHLGLGSSAIRSAELVMPDGRIRRIDAGSDPDLFWALRGGGGGFGVITELEIEL
                     TPVPRMAGGVLGWGIEDAERVLGAWSEWTRRIDDDVTSAVRFMHPPEGPALTVVVVTA
                     PRAAEELAAILKPLTDLKPMLNTVRDTDPEAFLREYTDPEIDPAEPPHA"
BASE COUNT          222 a          518 c          461 g          224 t
ORIGIN      
        1 ctggctgccg gacctggcga tcagctccag cgcctgcagc cgcaggtcgt cgagttcgtt
       61 gcggtattcg tcgatccagc ccgcctcgag cccgggcaac agcccgccgg aaaggatctt
      121 ctcggcttcg cccgccagct cgtaggcctc ccggtgccga ccggccgatt catccaggcg
      181 agcctcttcg atcccggcct gggctgcctc ccaatcgacc caggcatcat cgccgagggc
      241 gagctcgagt tcggagcggc cctcgagccg ccccgggcca agggccttgc gcaggcgcga
      301 aagagggggc gccagcagtg cctctccgcc acttggcaag ccctcttcgg cccaaagcgc
      361 ctcgaccagt tcatcccgtc tgaccggacg gtgccggttc agcgcaaggt acgcaaaaag
      421 cagcctgccc tggcgccccg gcagggattc gccgagatcc tcgccgtccc agttcacgtt
      481 caaacggccg caaagtgtga tccggggatt tctcatcgca aggtgacagt catttcgccg
      541 caatccgggc gcaacctgtg ccgtggattc tgccagccat gtcaacgcct tctctcataa
      601 acctccttcc cgcggcggcc cgcatggcgc cggaaacccc cgaagaagtc gcctcggccc
      661 tcgtcgaagc gggccgccag agtcgcaagg tggtcgtctt ctcgaccggg cacggcatcc
      721 cgccgatcgg tgacacctcg gatcgcgtcc tgctcgacat gagcgcattc gacaaggtcg
      781 agatcgatcc gctcgcccgg accgcccgga tcggcggcgc cacccagtgg atggagctga
      841 tctcggccgc caacgagcac gggctcaccg caccgttcgg atcggccgcc acggtcggcg
      901 tcaccggtta catgatgggt ggcggcgtcg gcttcatgtc ccgccacctg gggctgggca
      961 gcagcgcgat ccgctcggcc gaactggtca tgcctgacgg ccggatccgc cgcatcgatg
     1021 ccggatccga ccctgacctg ttctgggccc tgcgcggcgg cggtggcggc ttcggcgtga
     1081 tcaccgagct cgagatcgaa ctgacccctg tgccgcggat ggccggtggt gtgctcggct
     1141 ggggcatcga ggacgcggag cgggtgctcg gcgcgtggtc cgaatggacc cgccggatcg
     1201 acgacgacgt gaccagcgcg gtccgcttca tgcatccgcc cgaagggccg gccctgaccg
     1261 tggtggtcgt gaccgccccc cgcgcggcgg aagagctggc cgcgatcctg aagcccctga
     1321 ccgacctgaa gccgatgctg aacacggtcc gggacaccga ccccgaagct ttcctgcggg
     1381 agtacaccga cccggagatc gatccggcag agccgcccca tgcct
//
LOCUS       DAOQFI010000627         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_66259_length_1425_cov_2.024088, whole genome shotgun sequence.
ACCESSION   DAOQFI010000627 DAOQFI010000000
VERSION     DAOQFI010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66259_length_1425_cov_2.024088"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLE93_09945"
     CDS             complement(<1..395)
                     /locus_tag="PLE93_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87414.1"
                     /translation="MPTDLKSAVLLIVFNRPGTTKAVLEAIREVKPPRMYIASDGPRP
                     DRRGEEIEVENVRSLVAEGIDWDCEVKTLFRKENLGCRLAVSGAISWFFEQEEEGIIL
                     EDDCLPDRSFFFFCEDLLDRYRGDKRVMHV"
     gene            586..>1425
                     /locus_tag="PLE93_09950"
     CDS             586..>1425
                     /locus_tag="PLE93_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNH87415.1"
                     /translation="MNFGRLLDLARGPSAQTLATSIASQALTLISGVVVARALGVEGR
                     GVLALLWLLPLTIVLLGGIGIPQATTFYVARELDNARAVVSISIRISLLLAVILSLGY
                     ATGLLLLSDGQEFSASDALLSSALIPMFLAQNLGVAALLGMKRYRAFNAGRIVPALLY
                     ALLVTTLFGLSIATLSSILIATVGAWAFGALVTWLLVFRKLPSTGEVAGAATSDVLSF
                     GLRGVIGSVSPIDDVRIDQLMVGLLMDSRALGLYVAAIAFCNLPRFIAQSIGAVSFPR
                     IASA"
BASE COUNT          229 a          454 c          426 g          316 t
ORIGIN      
        1 acgtgcatca cccgcttgtc gccccggtac ctgtccagca ggtcttcgca gaagaagaag
       61 aagctgcgat ccggcaggca gtcgtcttcg aggatgatgc cctcttcctc ctgctcgaag
      121 aaccagctga tcgccccgct gacggcgagc ctgcacccca ggttctcctt ccggaagagt
      181 gtcttgacct cgcaatccca gtcgatgccc tcggcgacga ggctacgaac gttctctact
      241 tcgatttcct cgccccgacg gtcgggcctc ggcccgtcag acgcaatgta catccgcggt
      301 ggcttcactt cgcgaatcgc ctcgagaacc gccttggtcg tcccgggacg attgaaaacg
      361 atcagcagga ccgcggactt caggtcggtc ggcatcagcg ggccagtata tccgcgcaca
      421 gcttccggct tcctctctct ggctgacgga atcagggcgt gagtcatgtc cggattcagc
      481 tagcttcccg gggagttact ggaccgcagt ggtggtccag tccaagactt gggcgatcat
      541 caagagaaag aaacattcat gaagccagaa ggccggagag gcctggtgaa cttcggtcgg
      601 ctcctggacc tggcccgcgg gccgagcgcc cagaccctgg ctacctcgat cgcaagccaa
      661 gccctgactt tgatcagcgg ggtagtcgtc gcaagggccc ttggcgtcga aggccggggg
      721 gtccttgccc ttctttggct gttgccgttg acgatcgtgc tgttgggagg gatcggcatt
      781 ccccaggcca cgacgttcta cgtagcccgg gaactggaca acgccagggc cgtcgtgagc
      841 atttcaatcc ggatctcgct gctgctggcg gtgatccttt ctctcgggta cgcaaccggg
      901 ttgcttctgc tttcggacgg ccaggaattc tccgcctcgg atgccttgct cagttccgct
      961 ctgattccaa tgttcctggc acagaacctc ggggttgcgg ctctgctcgg catgaagcgt
     1021 taccgggcat tcaacgcggg acggatcgtt ccggcgcttc tctacgcgct cctggtgacc
     1081 actcttttcg gtcttagcat cgccactctt tcgtcgatcc tgattgccac agtcggcgcc
     1141 tgggcctttg gcgctctggt cacctggctc ttggtctttc gcaagctgcc gtcaaccggc
     1201 gaggttgccg gagcagccac ctcggatgtc ttgagcttcg ggctccgggg agtaatcggc
     1261 agtgtttcgc cgatcgacga cgtgcggatc gatcagctga tggtcggcct gctgatggac
     1321 agccgggcgc tcggtctgta cgtggctgcg atcgccttct gcaaccttcc gagattcatc
     1381 gcccagagca tcggcgccgt ctcttttccg cggatcgcat ccgcc
//
LOCUS       DAOQFI010000628         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_66318_length_1424_cov_2.855369, whole genome shotgun sequence.
ACCESSION   DAOQFI010000628 DAOQFI010000000
VERSION     DAOQFI010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66318_length_1424_cov_2.855369"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLE93_09955"
     CDS             <1..480
                     /locus_tag="PLE93_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNH87416.1"
                     /translation="GTGDIAMTLAQLLPQAKITIVTTPQPAAQSVARRSAEMAAKVDL
                     EVMGIIENMAGFTTPEGETFSIFGVGGGQLLADELEVPLFGSVPLSEELREHADVGNP
                     LSVDQPDSPAGQAIVEIARKIIATSPIELPMIETIQTTAPEIAPKMGKNARELPLVQ"
     gene            complement(624..887)
                     /locus_tag="PLE93_09960"
     CDS             complement(624..887)
                     /locus_tag="PLE93_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNH87417.1"
                     /translation="MSYVIAEPCIGQKDNSCVEVCPVDCIHPTPDEPGYDAVEMLFID
                     PDECIDCDACVEACPVDACFAEDQLPAEWAHFAEKNAAYFQNA"
     gene            999..>1424
                     /locus_tag="PLE93_09965"
     CDS             999..>1424
                     /locus_tag="PLE93_09965"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNH87418.1"
                     /translation="MILLTGATGSIGSRLLPRLIEDGHQVRCLVREPRKLGAHRVDVQ
                     ITMGDLRELRDPYMLRQAMRGVDTVIHLAASIRDQPPYRVEELNGLATVRLLEAAERA
                     GVKRFVFFSALNASAVQRTRFFRAKWTAEQAVANSRLDST"
BASE COUNT          252 a          483 c          449 g          240 t
ORIGIN      
        1 ggcaccggcg acatcgccat gaccctggcc cagctcctgc cgcaggcgaa gatcacgatc
       61 gtgaccacgc cgcagccggc cgcgcagtcg gtcgcccggc gttcggccga gatggccgcc
      121 aaggtcgacc tcgaggtgat gggaatcatc gagaacatgg ccggcttcac caccccggag
      181 ggcgagacct tctcgatctt cggcgtaggc ggaggtcagc tcctggccga cgaactcgag
      241 gtgccgctgt tcggatcggt gccgctctcg gaagagctcc gcgagcacgc cgatgtcggc
      301 aaccccctct cggtcgatca gccggattcc ccggccggtc aggcgatcgt cgaaatcgcc
      361 cgcaagatca tcgccacctc acccattgag ctcccgatga tcgaaacgat tcagaccacc
      421 gcccccgaaa tcgccccgaa aatgggcaag aacgcccgcg aacttcccct cgttcaataa
      481 aagccgggcg ggttagccgg tccatcaaat tcgggatcaa gtgggccggc gggcatctgc
      541 tccgggtcgg tcttgccggc cggtgcgaaa gctatgggag cgccagccct gctggcgcga
      601 ctgcagtcaa gccaaaacgc cgctcaggcg ttttggaagt aggccgcgtt cttctcggcg
      661 aagtgggccc attcggcggg gagctggtcc tcggcgaagc aggcatcgac cgggcacgct
      721 tcgacgcagg cgtcgcagtc aatgcactcg tcgggatcga tgaagagcat ttccaccgcg
      781 tcgtagcccg gctcgtcagg ggtcgggtgg atgcaatcca ccggacacac ctcgacgcag
      841 gagttgtcct tttgaccgat gcatggctct gcgatgacgt aactcatggt tcccatatta
      901 gagattgagg ccgcccgacc tccctctcgt tgacccgccg aatcactgcc tgatcacacg
      961 gaaacggccc gcaatcccgt cgctttagac tcctgcccgt gatccttttg accggagcga
     1021 caggctcgat cggctcgcgg cttctgccgc gcctgatcga agacggccat caggtccgct
     1081 gcctcgttcg cgaaccgcgc aagctcggcg cgcaccgggt tgacgtccag atcacgatgg
     1141 gggacctgcg cgaactgcgt gacccgtaca tgctgcgcca ggcgatgcgc ggggtcgaca
     1201 cggtgattca cctcgccgcg tcaatccggg accagccgcc ctaccgggtc gaggaactga
     1261 acgggctggc caccgtgcgg ctgctcgagg cggccgagcg ggccggggtg aagcgtttcg
     1321 tcttcttcag cgccctcaac gcctcggcgg tccagcggac ccgcttcttc cgggccaagt
     1381 ggacggccga gcaggcggtc gcaaattcac ggctcgactc gacg
//
LOCUS       DAOQFI010000629         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_66654_length_1420_cov_2.866667, whole genome shotgun sequence.
ACCESSION   DAOQFI010000629 DAOQFI010000000
VERSION     DAOQFI010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66654_length_1420_cov_2.866667"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLE93_09970"
     CDS             complement(<1..490)
                     /locus_tag="PLE93_09970"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNH87419.1"
                     /translation="MVERGDSSTHQGMVRIIENGQPLAEPFLTVDNVGLENERGLMSL
                     AFAPDYATSGLFYVFYVVNESGDAFGGDGGDIRIVEYQVSAGDPDKADPASARLVFST
                     PHYTTYHQGSWMAFGPDGHLYFTIGDDGYGPNAQLKDNYFGKIMRIDPADPDGNGPAS
                     HTV"
     gene            722..>1420
                     /locus_tag="PLE93_09975"
     CDS             722..>1420
                     /locus_tag="PLE93_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87420.1"
                     /translation="MLPVFLGVACLPFFASMASAGLVRTDSWGGGLVDPGFVVQSVAY
                     DSQGRAYIGESGKARVIRLAADGSVDLNWSVGQLPGEDEKKANPVDIAIDDEDNVYVL
                     DQIEQMVRKYTADGSLLDQWSAAVPCCLDVEGITVEAGSVFVISGYDVYRYTPSGVLL
                     DSWRGFDSYPEWEQPQIRGDGEGHLLIGSGRFLDEFKANKSIYKFEPDGTMVSQWTTP
                     AQGWGGPLRDHRAVG"
BASE COUNT          255 a          438 c          461 g          266 t
ORIGIN      
        1 gcaccgtgtg actggccggc ccgttcccgt cagggtcggc cgggtcgatc cgcatgatct
       61 tgccgaagta gttgtccttt agctgggcgt tcgggccgta tccgtcgtcg ccgatcgtga
      121 agtagaggtg gccgtccggg ccgaaggcca tccagctgcc ctggtgatag gtcgtgtaat
      181 ggggggtgct gaacacgagc cgggccgagg ccgggtctgc cttgtccggg tcacccgccg
      241 agacctggta ctcgacgatt ctgatgtcac cgccatcgcc gccgaaggcg tcgcccgact
      301 cgttgaccac gtagaagacg tagaagagcc cgctggttgc atagtccggg gcgaaggcca
      361 gtgacatcag gccgcgctcg ttttcgaggc cgacgttgtc cacggtcaga aagggctcgg
      421 ccaggggctg gccgttctcg atgatccgca ccatcccctg atgggtcgag ctgtcacccc
      481 gctcgaccac gaaaagcctc gaatcaccgg gggtgaagtc agaaataccg ggtcggaccc
      541 gaaatactcc tccggagtga tccgcttcag cccggccccg aatgacgcat cggccattcc
      601 gagcaggcaa gccaaagcca cccctgcgac aacgatccac ttcatgccac agaaactaca
      661 tcgaggccgt cgggttgtga tttgtgtgaa ccggtttcgt ccgaaaggtc cccttcgcct
      721 gttgctgccc gtgtttctcg gggtcgcctg cctgccgttc ttcgcctcaa tggcttcggc
      781 cggactggtc agaaccgact cctggggcgg gggtctcgtc gatcccgggt tcgtggtcca
      841 gtcggtcgcc tatgacagcc agggtcgcgc ctacatcggg gaatccggca aggcgagggt
      901 gattcgcctg gccgccgacg gttcggtcga cctcaactgg agtgtcgggc aactgcctgg
      961 cgaagacgag aagaaggcca acccggtcga catcgcgatc gacgatgaag acaacgtcta
     1021 cgtcctcgac cagatcgaac agatggtcag gaagtacacg gcagacggct ccctgctgga
     1081 tcagtggagc gccgccgttc cctgctgcct tgacgtcgaa ggcatcaccg tggaagccgg
     1141 ttcggttttc gtgatcagcg gctacgacgt ttaccgctac acgccgtccg gggtcctgct
     1201 ggacagctgg agggggtttg actcgtaccc ggaatgggag cagccgcaga tccggggcga
     1261 tggtgaaggc cacctgctga tcggcagcgg caggtttctc gacgaattca aggcgaacaa
     1321 gtcgatctac aagtttgaac ccgacggcac gatggtctcg cagtggacca cccccgccca
     1381 gggctggggt ggtccactgc gagaccatcg tgccgtcggg
//
LOCUS       DAOQFI010000630         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_66889_length_1417_cov_3.416300, whole genome shotgun sequence.
ACCESSION   DAOQFI010000630 DAOQFI010000000
VERSION     DAOQFI010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66889_length_1417_cov_3.416300"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLE93_09980"
     CDS             <1..997
                     /locus_tag="PLE93_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNH87421.1"
                     /translation="EAEANREMDRWPVDEPFPTLKSFNSITLRVILRAVFGAEGKELA
                     ELEQIIPPITRYGQMRVASSLLMRDYGRFSPGGRGERLMTRFHEIIRTLIDKHLADPG
                     LADRIDILALMLNSDLQEGRAVDYQATGEELMTILVAGHETTASSLAWNIERITRHPE
                     VLDRLTAEARTDQNEYRLAVINEVLRTRPVIGMTGRSVFAESFELDGWKLPAGTRILC
                     NIRETNVDPSAHPEADRFNPDRYVGKKPDTYSWIPFGGGLRRCLGAAFAQMEMDIVLR
                     TLLRRFDLAATSRPPEGERFKGLAYAPSRGGVAMVRYRDGKPPARLEPQVAAGAR"
     gene            1135..>1417
                     /locus_tag="PLE93_09985"
     CDS             1135..>1417
                     /locus_tag="PLE93_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87422.1"
                     /translation="MLRAAVASAAIALLVADHYLHMDHWVIICSALVLLVLICGGLVI
                     AIGSARRNVRSEALNRIKEEENLHLADIVRSSKDAIFSVDLDGKVTAWNE"
BASE COUNT          262 a          469 c          440 g          246 t
ORIGIN      
        1 cgaagccgag gcgaaccgcg aaatggaccg gtggccggtc gacgagccgt tcccgacctt
       61 gaagtccttc aattcgatca ccctgcgggt cattctccgg gcggtgttcg gggccgaggg
      121 caaagagctt gccgaactgg agcagatcat cccgccgatc acccgttacg gccagatgcg
      181 ggtcgcctcc agtctgctga tgcgcgacta cgggcggttc agcccgggcg gtcgcggaga
      241 gcggctgatg acccggttcc acgaaatcat ccggaccctg atcgacaagc acctggccga
      301 ccccggcctc gccgaccgga tcgacatcct cgccctgatg ctgaactcgg acctgcagga
      361 ggggagagcc gtcgattacc aggcgaccgg ggaagagctg atgaccattc tggttgccgg
      421 tcacgaaacg accgcttcct cactggcctg gaacatcgag cgcatcacca ggcaccccga
      481 ggtgctggac cggctcaccg ccgaagcccg gaccgatcag aacgagtacc ggcttgcggt
      541 tatcaacgaa gtcctcagaa cccgcccggt gattggcatg accgggcgtt cggtcttcgc
      601 cgagagcttc gagctcgatg gctggaagct gccggccggc accaggatcc tctgcaatat
      661 ccgggaaacc aacgtggatc cttcagctca ccccgaagcc gatcgcttca acccggaccg
      721 ctacgtcggc aagaagcccg acacctattc ctggattccc ttcgggggcg ggctgcgccg
      781 ctgccttggc gccgccttcg cccagatgga aatggacatc gtgctcagga ccctgcttcg
      841 tcgtttcgac ctcgccgcaa ccagccggcc gcccgaaggc gagaggttca agggactcgc
      901 ctacgccccg tccaggggcg gggtggcgat ggtccgctac cgcgacggca agccgcccgc
      961 ccggctggag ccgcaggtgg ccgccggagc acgctaggcc atcgcggtta tcttttagct
     1021 gagcctcagg gggcgtcgcc cacgctcgac gatcgagcaa ttttgaaacg gcaatcaaga
     1081 tccgcatcag tgagggcact ctttcgaacg gatccgttcg gggcacggtt tgccgtgctg
     1141 cgtgcagcgg tcgcgagtgc cgcgatcgca ttgctggttg ccgaccacta ccttcacatg
     1201 gatcactggg tgatcatctg ttcggcgctg gtcctgctcg tcctgatctg cggcggcctg
     1261 gtcatagcaa tcggttccgc ccgccggaat gtccgcagcg aagccctgaa ccggatcaag
     1321 gaggaagaga acctccacct cgctgacatc gtcagatcct ccaaggacgc aatcttctcg
     1381 gttgacctcg acggcaaggt gaccgcctgg aatgaag
//
LOCUS       DAOQFI010000631         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_66947_length_1417_cov_1.676211, whole genome shotgun sequence.
ACCESSION   DAOQFI010000631 DAOQFI010000000
VERSION     DAOQFI010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66947_length_1417_cov_1.676211"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..>1417
                     /locus_tag="PLE93_09990"
     CDS             141..>1417
                     /locus_tag="PLE93_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH87423.1"
                     /translation="MTDTPINSPNGSGKPPWYRQRGKVISLIAVVLVAAGAIALVAWN
                     ELKRPDDVSNPDVPFVKTKPKQVVPKDRTINWSTFRLNPQRTGYLPVNGIHPPFKRVW
                     KYGDQPLLEFPPIVVDGRLYFVDNDGNARALRADNGREIWHRKIADLNASTPTYARGR
                     LYIVNLEPGHVLSMNAKNGRIIWEKPLPCRSESSPAVIRQRVYFGCENGVLYALKARN
                     GKEVWETSVDGAIKGAPAYEDGTLFVGDYGGVVSAVRAKTGEIKWQSDSLGGSFGRAG
                     SFYSTPAVAYGRVYLGSNDGRVYSFDKNTGELAWTYSTGDWVYSGPSVAQVPGTPPTV
                     YIGSFDGNVYALNARSGEARWTFPMGGQVIGSLSVIGRTVYAATFDGTTTYGISAKSG
                     RKVFEFHTGAYMPGISDGEKLYLVGYSSIHAMKP"
BASE COUNT          262 a          463 c          437 g          255 t
ORIGIN      
        1 accaggtaga ccggctgttc ggagatctgg cgaacctgat cgatgaccgg atgcgtccgg
       61 atcagtgttg ccaggcggtt gaagtcgggg tcgattcctt gtgccatctc tcttctcccg
      121 taccatgatc ccttcttcgg atgaccgaca caccaatcaa ctctccaaac gggtcgggca
      181 agcccccctg gtaccggcag cgcggcaagg tcatttcgct gatcgcggtg gtcctggtgg
      241 ccgccggcgc gatcgccctt gtcgcctgga atgagctgaa gcggcccgat gacgtcagca
      301 atcccgacgt cccgttcgtc aagaccaagc cgaagcaggt cgtgccgaag gacaggacga
      361 tcaactggtc cactttccgc ctcaaccccc agcgcaccgg ctatctgccg gtgaacggca
      421 tccatccgcc cttcaagcgg gtctggaagt acggcgacca gccgctgctc gagttcccgc
      481 cgatcgtggt cgatggccgg ctttacttcg tcgacaacga cggtaacgcg agagccttgc
      541 gggccgacaa cggacgggag atctggcatc gaaagatcgc cgacctgaat gcctcgaccc
      601 cgacctatgc caggggccgc ctttacatcg tgaatctcga gcccggccac gtgctttcga
      661 tgaacgcgaa gaacggccgg atcatctggg agaagccttt gccctgccgt tccgaatcct
      721 cgcccgcggt gattcgccag cgggtgtact tcggttgcga gaacggggtg ctgtatgcgc
      781 tcaaggcccg aaacggcaag gaagtgtggg aaacctcggt cgacggggcg atcaagggag
      841 caccggccta tgaggacggc accctcttcg tgggcgacta cggaggcgtc gtttcggcgg
      901 tgcgggcaaa gaccggtgaa atcaagtggc agtcggacag cctcggcggc agcttcggcc
      961 gggccggttc cttctattcg acaccggcgg tcgcctacgg ccgcgtatat ctcggcagca
     1021 acgacggccg ggtctacagc tttgacaaga acaccggcga acttgcctgg acctactcga
     1081 ccggcgactg ggtctactcg ggcccgtcgg tcgcccaggt tccgggcact ccgccaaccg
     1141 tctacatcgg ctccttcgac ggcaacgtct acgccctcaa cgcccggtcc ggcgaggcac
     1201 gctggacctt cccgatgggc ggccaggtga tcggttcgct cagcgtaatc ggccgaacgg
     1261 tctacgccgc gaccttcgat ggcaccacca cctacgggat cagcgccaag tccggacgca
     1321 aggtcttcga gttccacacc ggcgcctaca tgccggggat ctcggatggc gagaagctct
     1381 atctggtcgg ctactcgagc atccacgcga tgaagcc
//
LOCUS       DAOQFI010000632         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_67060_length_1415_cov_3.654412, whole genome shotgun sequence.
ACCESSION   DAOQFI010000632 DAOQFI010000000
VERSION     DAOQFI010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67060_length_1415_cov_3.654412"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLE93_09995"
     CDS             <1..208
                     /locus_tag="PLE93_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH87424.1"
                     /translation="SPWVDSYGAGDSFAAGITYGLGLGLPVLEAARIGALCGAANLQG
                     RGPYAGQATAEDLARFDLAQDPPG"
     gene            complement(190..>1415)
                     /locus_tag="PLE93_10000"
     CDS             complement(190..>1415)
                     /locus_tag="PLE93_10000"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNH87425.1"
                     /translation="KSGGAGEGSMALAASARYVQADVTDVEGVRSVLAAVEGQPIIYF
                     ALPPEVSITACEVLRKVGCPPETRLVLEKPFGRDAESAEAFNRLLAEIVPESQVHRVD
                     HFLGLSTVLNIFGLRFANRVFEPMMNSHHVESVEILWDEDLALEGRAGYYDRAGALVD
                     MIQSHLLQVAAVLAMEPPPTTGEKDIRDGTARILRAASVWDDDPARFSRRARYSAGTI
                     KGRELPAYADEEGVEPEKMTETFAEIVIGVDTWRWAGVPFRLRSGKAIGNPRKEAVIN
                     FRDPEQIPRGLVGAEGQNRLRIGLGFGSDTVRFDIAVNGPGDPKELDFATLEATLGAG
                     YLSEYGEVLKTVLDGTPALSVRGDAAVDCWRIIDPVRQAWKTGEVPLLEYPAGSSGPE
                     PWPESGLPGSQPGGS"
BASE COUNT          232 a          490 c          446 g          247 t
ORIGIN      
        1 gtcgccgtgg gtcgacagct acggggcagg tgactcattc gccgccggaa tcacttacgg
       61 cctcggtctc ggacttccgg ttctcgaggc agcccggatc ggtgcgctct gcggggccgc
      121 gaacctgcag gggcggggtc cctacgccgg gcaggcgacg gccgaagacc tggcccggtt
      181 cgatttggct caggacccgc cgggctgaga tcccggaagg ccgctctccg gccagggctc
      241 cggtccgctc gagccggccg ggtactcgag caggggcacc tcgcccgtct tccatgcctg
      301 ccggaccggg tcgatgatcc gccagcagtc gaccgccgca tcgccacgca ccgaaagtgc
      361 cggggtgccg tcaagcaccg tcttcagcac ctcgccgtac tcggacagat acccggcgcc
      421 aagcgtcgct tcgagagttg cgaagtcgag ttccttcggg tcgccgggac cgttcaccgc
      481 gatgtcgaac cgcaccgtgt cggagccgaa gccgagaccg atcctcagac ggttctgacc
      541 ctctgcgccg accagccctc tcggtatctg ttccggatcg cggaagttga tgaccgcttc
      601 tttccgtgga ttgccgatgg ccttgcccga gcgcaggcga aagggaaccc cggcccacct
      661 ccaggtatcg acaccgatca cgatctcggc gaacgtttcg gtcatcttct ccggctcgac
      721 cccttcctcg tctgcatagg cgggaagttc gcggcccttg atcgtccccg ccgagtacct
      781 cgcccggcgg ctgaacctgg ccgggtcgtc atcccagacc gaagcggcgc gaaggatcct
      841 ggcggtgccg tcacggatgt ccttctcgcc ggtcgtcggt ggcggttcca tcgccagcac
      901 cgcagccacc tggagcagat ggctttggat catgtcgacc agcgcaccgg cacggtcgta
      961 atagccggct cgcccttcca gggcgaggtc ctcatcccag aggatctcga cgctctcgac
     1021 gtgatgggag ttcatcatcg gctcgaagac ccggttcgca aaccggagac cgaagatgtt
     1081 cagcaccgtg gagaggccga gaaagtggtc aacgcggtga acctggctct ccggaactat
     1141 ctccgccagc aaacggttga aggcttcagc cgattccgcg tcccggccaa acggcttttc
     1201 gagaacgagc cgggtttcag gtggacagcc gaccttccgc agtacctcac aggcggtgat
     1261 cgacacctcg ggcggcaacg cgaaatagat gatcggctgg ccttcgaccg cggccagaac
     1321 gcttctcacg ccttccacgt cggtgacgtc ggcctggacg tagcgggcgg aggcggcgag
     1381 tgccatggac ccctcccccg ctcctcccga cttct
//
LOCUS       DAOQFI010000633         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_67067_length_1415_cov_3.123529, whole genome shotgun sequence.
ACCESSION   DAOQFI010000633 DAOQFI010000000
VERSION     DAOQFI010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67067_length_1415_cov_3.123529"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLE93_10005"
     CDS             complement(<1..281)
                     /locus_tag="PLE93_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNH87426.1"
                     /translation="MPRILIVTWEYPPLIEGGLARHVRKLSEALVGLGVEVHVLTRGG
                     EQATIEEEMAGVQIHRIVEPERPTDLAEFVTWVERMNAELGVAMDEPAVV"
     gene            complement(297..>1415)
                     /locus_tag="PLE93_10010"
     CDS             complement(297..>1415)
                     /locus_tag="PLE93_10010"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNH87427.1"
                     /translation="VPATRAEVDRYEKAAVLLERVGGSPLAAFAAVRDRNVSLIPSSA
                     THAVLPLLATRAGLDLQIDAGLASHRRRFGPSSGFWLPECAYRPGLEDALARHRIEWF
                     CVDQSRHESGDAALAPVRTPAGPLAFTIDWPAISWLWDMSGYPSWGGYLDFHRLSMNG
                     VRLFAIDGGPYEPEAARQRADRQAGEFLDSVRERLEEYRQRTGQRGLMTFAIDTELLG
                     HWWSEGPDWLAAVLDQAGERGVRAVNLEQAAREHGPSARQSSLKASTWGEAKDFHTWD
                     SPAVADLAWASRGLELRVGEAIGSLPREQAERAVRELLMVQSSDWAFLDHRGQAGDYP
                     FERATGHAESAFEALQSPIGSRLRGLAPDLSLTPFLTP"
BASE COUNT          241 a          486 c          488 g          200 t
ORIGIN      
        1 acgaccgcgg gctcgtccat cgccaccccg agctcggcgt tcatgcgctc gacccaggtc
       61 acgaactcgg ccaggtcggt cgggcgttcc ggttcgacga tccggtggat ctggaccccg
      121 gccatctctt cctcgatggt cgcctgctca ccgccgcggg tcagcacgtg aacctcgacc
      181 ccgagcccga ccagtgcttc ggagagctta cgcacatgcc gggccaggcc gccctcgatc
      241 agcggcgggt actcccaggt gacgatgagg atgcgaggca aggtggtggc tgaaggctag
      301 ggcgtgagaa acggtgtgag actcaggtct ggagcaaggc cgcgcaaacg cgaaccgatc
      361 ggggattgta aggcttcgaa ggcgctttcc gcatggccgg tggcccgctc gaagggatag
      421 tcgccggcct ggccgcggtg gtcgaggaag gcccagtcgc tggactgaac catcaggagc
      481 tcgcgaacgg cccgctcggc ctgttcccgg ggcagggacc cgatcgcctc ccccaccctc
      541 agctccaggc cccgcgaggc ccaggcgagg tcggccacgg caggcgaatc ccaggtgtgg
      601 aagtccttag cctcgcccca ggttgaagct ttcagactgc tttggcgggc cgacgggcca
      661 tgttccctcg ccgcctgttc gaggttgacg gcacggaccc ccctctcccc ggcctggtca
      721 aggaccgcgg ccagccagtc cggaccctcc gaccaccaat gtccgagcag ttcggtgtcg
      781 atcgcaaagg tcatcagccc ccgctgcccg gtgcgctgtc ggtactcctc aaggcgctcc
      841 cggaccgagt cgaggaactc gcccgcctgc cggtccgccc gctggcgggc tgcctccggt
      901 tcgtagggac caccatcgat cgcaaagaga cgaaccccgt tcatggaaag gcgatggaag
      961 tccaggtagc cgccccagga cgggtagccg gacatatccc aaagccagga gatcgccggc
     1021 cagtcgatcg tgaaggcgag cggaccggcc ggggtccgga ccggcgcgag ggcggcgtcg
     1081 ccggattcgt gccggctctg gtcgacgcag aaccactcga tccggtgccg ggcaagagca
     1141 tcctccagac ccggacgata ggcacattcg ggaagccaga atccggatga agggccgaag
     1201 cggcgccggt gagaggcgag accggcgtcg atctggaggt cgagcccggc ccgggtcgcc
     1261 agcaggggca ggaccgcatg agtggcgctg gaggggatca gggaaacatt ccggtcccgg
     1321 accgcggcga acgcggccag cggactgccg ccgacccgct cgagcaggac cgccgccttc
     1381 tcgtaacggt cgacttccgc ccgggtcgcc ggcac
//
LOCUS       DAOQFI010000634         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_67890_length_1406_cov_2.671355, whole genome shotgun sequence.
ACCESSION   DAOQFI010000634 DAOQFI010000000
VERSION     DAOQFI010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67890_length_1406_cov_2.671355"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLE93_10015"
     CDS             <1..577
                     /locus_tag="PLE93_10015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH87428.1"
                     /translation="AAAGLSERIEVVETDYRDLTGSFDRLVSIEMIEAVGWRDFPTYF
                     AKCSELLTEDGSMLLQAITIDDDAYELEKASRSFISRYIFPGGCLPSIREIRRCLDQV
                     TDLTIAWSDELRLDYARTLAEWRRRFNAVTGRLESLGYDDRFRRMWVMYLCLAEAGFR
                     SGRNSDFQLLLTKPRSPRVVALAPASAKEAV"
     gene            696..>1406
                     /locus_tag="PLE93_10020"
     CDS             696..>1406
                     /locus_tag="PLE93_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87429.1"
                     /translation="MSERDVEPVVLTADETPRLLGVAPDEVVAFAWFEGWSKVPTGWF
                     QVPVQVDERKMIDYRPISQSDMDGPGLRGMAYADPYTWAEADGVPQTATDLEDPGHGP
                     AIPGTAGDPNLDGNDEIAMMAYEGGLPAGDRPAPEGTDPDSRTPVRFTDPLHPGEHAY
                     IYLFRSNGSLAQDAGEQLVNYRRRFVPRLAGGYRFGYDFEGTSAKAGRGPANREDSTV
                     LSTRYDTRMPGRWMLDHLV"
BASE COUNT          239 a          443 c          477 g          247 t
ORIGIN      
        1 cgcggcggcg ggtctctccg aacggatcga agtggtggaa accgactacc gcgacctgac
       61 cggaagcttt gaccgcctgg tctcgatcga gatgatcgag gcggtcggct ggcgcgactt
      121 cccgacctac ttcgccaagt gctcggagct gctgacggaa gacggctcga tgctgcttca
      181 ggcgatcacg atcgatgacg acgcctacga gctggagaag gcgtcacgga gtttcatcag
      241 ccgctacatc tttcccggcg gttgcctgcc ctcgattcgg gaaatccgcc gctgtctcga
      301 ccaggtgacc gacctgacca tcgcctggag cgacgagctc cgccttgact atgcccgcac
      361 cctcgccgag tggcggcgcc ggttcaatgc ggtgaccggc cgtctcgaat cgctcggcta
      421 cgacgatcgc ttccgccgca tgtgggtgat gtatctctgc ctggccgagg ccggtttccg
      481 ttccggccga aacagcgact tccagctgct cctgaccaag ccgcgttcgc cgagagtcgt
      541 cgctctggcg ccggcatccg ccaaagaagc ggtttgaatg aaagtgcgtc gggtgtctcg
      601 gccgcatggc cagggtttgc agggtcctgt cagccatgct gttggttggt tgtctgagcg
      661 ggatcggata cggatccgcg caagcggatg atgttttgag cgagcgtgat gtcgagccgg
      721 tcgtcctgac ggcagacgaa acgccgcgac tcctcggtgt ggcaccggac gaggttgtgg
      781 cattcgcctg gttcgagggc tggagcaagg tgccgaccgg ctggttccag gtcccggtgc
      841 aggtggacga gcggaagatg atcgattacc ggccgatcag ccagagtgac atggacggtc
      901 ccgggttgcg gggcatggcc tacgccgatc cgtacacctg ggccgaggcc gacggtgtgc
      961 cgcaaacggc gaccgacctg gaagacccgg gtcacggtcc ggcgatcccc ggaactgccg
     1021 gtgacccgaa cctggatggc aatgacgaga tcgcgatgat ggcctacgaa ggcggcctgc
     1081 cagcgggcga ccggcctgcc ccggaaggca ccgacccgga ttcgaggaca ccggtccgct
     1141 tcaccgatcc gctccacccg ggcgagcacg cctacatcta ccttttccgc agcaacgggt
     1201 cgctggccca ggacgcgggc gagcagctgg tcaactacag gcgaagattc gtgccccggc
     1261 tcgccggcgg gtaccggttc ggatatgact tcgaagggac ttccgcgaag gccggtcgcg
     1321 ggccggcgaa ccgcgaggat tcgacggtct tgagcacgcg ctatgacacg cggatgcccg
     1381 gccgctggat gctcgatcac ctcgtg
//
LOCUS       DAOQFI010000635         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_68005_length_1404_cov_5.197183, whole genome shotgun sequence.
ACCESSION   DAOQFI010000635 DAOQFI010000000
VERSION     DAOQFI010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68005_length_1404_cov_5.197183"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLE93_10025"
     CDS             <1..336
                     /locus_tag="PLE93_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87430.1"
                     /translation="GTNISEPETVVTPFSEASVAVKIDRTGPAKPKIVLKGHKVKGQF
                     RGPVRVKVIGKPDGKLPDGTAGVGLNPKSVPKTRKVTRKGKTVIKIRTRDKLGNQSPV
                     AKVVIRIRR"
     gene            complement(344..1111)
                     /locus_tag="PLE93_10030"
     CDS             complement(344..1111)
                     /locus_tag="PLE93_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH87431.1"
                     /translation="MSPSMVRLVSLTRREVSRFLKIPRQTVGAPLLETFLYISIFGAA
                     LGSRVGDLHGYEYIVFVVPGLIMMAFAINAFTNNSSSILQQKFQGAIDDQLSSPASPG
                     QLLLAFTLGGFLRGLLISVVTFLVASLLIDLPVKHFYILIPALFLVGWFFASLGVLVG
                     VRAEQFDDVSFAQTFILQPLIFLGGVFYSASLLPEPYETLTHFNPVYYMISLVRYGFL
                     GVADISVWLSLALLTAATAALTFANYRMFKSGYKLRR"
     gene            complement(1108..>1404)
                     /locus_tag="PLE93_10035"
     CDS             complement(1108..>1404)
                     /locus_tag="PLE93_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH87432.1"
                     /translation="DEPTAGVDVELRLELWHYVQQVNSEGTSILLTTHYLEEAEQLCS
                     RIAFINGGEIVDHGSSDELAERFGVGSLEDAYLELVGRKELSRAHVGIEAEDGQ"
BASE COUNT          308 a          405 c          486 g          205 t
ORIGIN      
        1 ggcaccaaca tctccgagcc ggagaccgtc gtgaccccgt tctcggaagc cagcgtcgcg
       61 gtcaagatcg accggaccgg accggcgaag ccgaagatcg tgctcaaggg tcacaaggtc
      121 aaaggccagt tcaggggccc ggtccgggtc aaggtgatcg gcaagccgga cggcaagctg
      181 ccggacggga ccgccggcgt cgggctcaac ccgaaatccg tgccgaagac ccgcaaggtc
      241 acccgcaagg gcaagacggt gatcaagatc aggacccgcg acaagctcgg caaccagtcc
      301 ccggtcgcca aggtggtcat caggatcagg cgctgatccg tcgttatctc cggagcttgt
      361 agccggactt gaacatccgg tagttggcga aggtgagtgc ggcggtggcc gcggtgagca
      421 gggcaagtga aagccagacg ctgatgtcgg cgaccccgag aaacccgtaa cggaccaggg
      481 agatcatgta gtagaccggg ttgaaatggg tcagggtttc gtagggctcg ggcaggagcg
      541 aggccgagta gaacacgccg ccgaggaaga tcagcggctg gaggatgaag gtctgggcaa
      601 acgagacgtc gtcgaactgt tccgccctga ccccgaccag cacaccgagg ctggcgaaga
      661 accagccgac cagaaacagg gcgggaatga ggatgtagaa gtgcttgacc ggcaggtcga
      721 tcagcaggct ggcgaccagg aacgtgacga ccgagatcaa gaggccgcgc aggaaacccc
      781 cgagcgtgaa ggccaggagc aactggccgg gcgaggccgg ggaggagagc tggtcatcga
      841 ttgccccctg gaacttctgc tgaaggatcg atgacgagtt gttggtgaag gcgttgatcg
      901 cgaaggccat catgatcagg cctgggacca cgaagacgat gtattcgtaa ccgtgaaggt
      961 cacccacccg ggaaccgagc gcggcaccga agatcgagat gtaaaggaag gtctcgagca
     1021 acggtgcgcc gaccgtctgg cgggggatct tgagaaagcg gctgacctcg cgcctggtca
     1081 gggagaccag ccgcaccatc gacgggctca ctggccgtcc tccgcttcga ttccgacatg
     1141 ggcacgggaa agctccttgc ggccgaccag ttccaggtag gcgtcctcga ggctgcccac
     1201 cccgaaccgc tcggccagct cgtcgctgga accgtggtcg acgatctcgc ccccgttgat
     1261 gaaggcgatc cggctgcaga gctgctcggc ttcctcgagg taatgggtgg tgagcaggat
     1321 cgaggtgcct tcggaattga cctgctggac gtaatgccag agctccaggc gaagctcgac
     1381 gtcaaccccg gcggtcggtt cgtc
//
LOCUS       DAOQFI010000636         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_68115_length_1403_cov_4.523739, whole genome shotgun sequence.
ACCESSION   DAOQFI010000636 DAOQFI010000000
VERSION     DAOQFI010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68115_length_1403_cov_4.523739"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1012
                     /locus_tag="PLE93_10040"
     CDS             2..1012
                     /locus_tag="PLE93_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH87433.1"
                     /translation="MESADEAAAADDAVGEGEAELLAGEANEETTGPIDAAVTNEPEP
                     SNGKGFVASDGAVLGRDFVIPGVGPGDNDPYKWHTGPKQKTTDNAIEIVDVVKQFGRT
                     RILNGLNLGLPDDQISMVLGPSGTGKSVLIKHIVGLLYPDHGDVIVKGDSVPDLSDDD
                     LFEMRKKFGLLFQDGALFGSMNIYDNVAFPLRQHTDKGEEEIESIVTRRLREVGLGEA
                     HFKMPNELSGGMRKRAGFARALVLDPEIVMFDEPDSGLDPVRTALLCELIKEVHAESG
                     GCYMVISHDLGTARRIADFIAVLWKGRIVESGPKDELFESDNEFVHQFLQADVVGPLA
                     MD"
     gene            1014..>1403
                     /locus_tag="PLE93_10045"
     CDS             1014..>1403
                     /locus_tag="PLE93_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87434.1"
                     /translation="MSRFFGGVAAATARRPWPVLILAVLLGAISIWAAGGMGSQNVTD
                     AFFDKGSSAYRQTEQADRYFGTDPVVIMAKGPLIETLDTYNLNRLATLETCLAGRIKR
                     GRGQLFRTCKEISDLHPVHTLAGPATFL"
BASE COUNT          263 a          463 c          445 g          232 t
ORIGIN      
        1 gatggaatcc gccgacgagg ccgccgctgc cgatgacgca gtgggggagg gtgaagccga
       61 actgctcgcc ggggaagcca acgaggagac caccggtccg atcgatgccg cggtgaccaa
      121 cgagcccgag ccctccaacg gcaagggttt cgtcgccagc gacggtgccg tgctcggtcg
      181 cgacttcgtg attcccggtg tcgggcccgg tgacaacgac ccttacaagt ggcacacggg
      241 accgaagcaa aagaccaccg acaatgcgat cgagatcgtc gacgtggtca agcagtttgg
      301 ccggacccgg atcctcaacg gcctcaacct gggcctgccg gacgatcaga tctcgatggt
      361 gctgggcccc tcgggcaccg gcaagagcgt gctgatcaag cacatcgtcg ggctgctcta
      421 ccccgaccac ggagacgtga tcgtcaaggg tgactcggtt cccgacctca gtgacgacga
      481 cctcttcgag atgcgcaaga agttcggcct gctcttccag gacggggcgc tcttcggctc
      541 gatgaacatc tacgacaacg tcgccttccc gctccggcag cacaccgaca agggtgagga
      601 agaaatcgaa tcgatcgtta cccgccggct gcgcgaggtc ggtctcggtg aagctcactt
      661 caagatgccg aacgagcttt ccggcggcat gcgcaagcgc gccggatttg cgcgagccct
      721 cgtgctcgac ccggagatcg tcatgttcga cgagcccgac tcgggtcttg acccggtgcg
      781 gacggcgctg ctttgcgagc tgatcaagga agtccatgcc gaatcaggcg gctgctacat
      841 ggtgatcagc cacgacctcg gcaccgcgcg ccggatcgcc gacttcatcg cggtgctctg
      901 gaagggccgg atcgtcgaaa gcggaccgaa ggacgaactc ttcgaatcgg acaacgagtt
      961 cgtccaccag ttcctgcagg ccgacgtggt cggcccgctc gcgatggact gagatgtcga
     1021 ggtttttcgg cggtgtcgcc gcggccaccg cccgtcggcc ctggccggtg ctgatccttg
     1081 ccgtactgct gggcgccatt tcgatctggg cggccggcgg aatgggcagc cagaacgtca
     1141 ccgacgcctt cttcgacaag gggtcgagcg cctaccgcca gaccgagcag gccgaccgct
     1201 acttcggcac ggatccggtc gtgatcatgg ccaaggggcc cctgatcgag acgctggaca
     1261 cctacaacct gaatcgcctg gccaccctcg agacctgtct ggccggaagg atcaagcggg
     1321 gcagaggtca gctcttccgc acctgcaagg aaatctcgga cctccacccg gtccacaccc
     1381 tggccggtcc tgccaccttc ctc
//
LOCUS       DAOQFI010000637         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_68128_length_1403_cov_3.399852, whole genome shotgun sequence.
ACCESSION   DAOQFI010000637 DAOQFI010000000
VERSION     DAOQFI010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68128_length_1403_cov_3.399852"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLE93_10050"
     CDS             <1..330
                     /locus_tag="PLE93_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNH87435.1"
                     /translation="ARVREETATHGTREGMLRGLAVTGGVITSAGIVLAGTFSVLAVL
                     PLVFLAEMGFTVAFGVLLDTFLVRSILVPALTLDIGSKIWWPSALAEVGDDPASGKPV
                     EPDPAAG"
     gene            392..817
                     /locus_tag="PLE93_10055"
     CDS             392..817
                     /locus_tag="PLE93_10055"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNH87436.1"
                     /translation="MAARGSAEWQGTIREGSGTFTAGDSLGGEYSFKSRFEDGPGANP
                     EQLIAGAHASCFSMALSLVLEEAGTPVESVKTDAKVTIRMLEEGPTITRIELSTVGRV
                     PGADQAAFEEAAGKAKAGCPVSKALAGVPEITLEATLEP"
     gene            complement(850..>1403)
                     /locus_tag="PLE93_10060"
     CDS             complement(850..>1403)
                     /locus_tag="PLE93_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87437.1"
                     /translation="HVLRAHGEALTAQLDGLGDPVMAQWGMMPGPAVASLHGDPEDPV
                     PAIGNSTMLDGVGDELVEAFVGAASGESGCTLIAAELRQLGGALRRPDPDGGVASMID
                     GEFIAFGVGLAPEPGMKAKTEEDLTRLMEAVKPWEGGRQYYNFAVGGAACQDCFPEDD
                     IEALKAIRHEYDPESLYLAPLPL"
BASE COUNT          213 a          480 c          500 g          210 t
ORIGIN      
        1 gcccgggtgc gcgaggaaac cgcgacccac ggcaccaggg aaggcatgct gcggggcctc
       61 gcggtgaccg gcggggtgat tacctcggcc ggcatcgtgc tggccggaac cttctcggtg
      121 ctggccgtgc tgccgctcgt cttcctggcc gagatgggct tcaccgtcgc cttcggcgtg
      181 ctgctcgaca cgttcctggt ccgttcgatt ctggtcccgg ccctgaccct ggacatcggt
      241 tcgaagatct ggtggccttc cgcgctggcc gaggtggggg atgaccccgc ttccggaaaa
      301 ccggtcgagc cggacccggc cgccggttag tctgaacctc taccgggaac cagtcggaca
      361 cccggggcaa ccgaccagtc gaggaggacc aatggcagca cgaggaagcg cagagtggca
      421 gggcacgatc cgggagggca gcggcacctt caccgccggt gactcgctgg gaggcgagta
      481 ctcgttcaaa tcaaggttcg aggacggtcc cggggccaac ccggagcagc tgatcgccgg
      541 cgcccacgcg tcatgctttt cgatggcact ttccttggtc ctcgaggagg cgggcacacc
      601 ggtcgaatcg gtcaagaccg acgccaaggt gacgatcagg atgctcgaag agggcccgac
      661 gatcaccagg atcgagctct ccacggtggg ccgcgtgccc ggcgccgatc aggctgcctt
      721 cgaggaggcg gccgggaagg ccaaggccgg ctgcccggtc tccaaggccc tggccggggt
      781 gcccgagatc accctcgaag cgaccctcga gccctgaggc cgagggcggc cccttccggc
      841 cgggccatct cagaggggta gcggcgccag gtagaggctc tcggggtcgt attcgtgacg
      901 gatcgccttg agggcctcga tgtcgtcctc ggggaaacag tcctggcagg ccgccccgcc
      961 gaccgcgaag ttgtagtact ggcggccgcc ctcccagggc ttgaccgcct ccatcaggcg
     1021 ggtcaggtcc tcctcggtct tcgccttcat gcccggctcg ggggcgaggc cgaccccgaa
     1081 agcgatgaac tcgccgtcga tcatgctggc gacgccgccg tcagggtcgg gccggcgcag
     1141 ggcgccgccg agctggcgca gttccgccgc gatcagggtg cagcccgatt ccccgctggc
     1201 ggccccgacg aaggcctcga ccagttcgtc gccgaccccg tcgagcatgg tcgaattgcc
     1261 gatcgccggc accgggtcct cggggtcgcc gtgaagcgag gcgacggcgg ggccgggcat
     1321 catgccccac tgggccatga ccggatcacc aaggccgtcc agctgggcgg tcagggcctc
     1381 gccgtgcgcc cgaaggacat gcg
//
LOCUS       DAOQFI010000638         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_68197_length_1402_cov_3.948775, whole genome shotgun sequence.
ACCESSION   DAOQFI010000638 DAOQFI010000000
VERSION     DAOQFI010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68197_length_1402_cov_3.948775"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PLE93_10065"
     CDS             <1..1082
                     /locus_tag="PLE93_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNH87438.1"
                     /translation="RKELAAAIAEHELVAEHGNPNETGIRLTTNSGIGVDVRIVEPGA
                     FGHLLQHFTGSGPHNAELRERAVAQGLHVSENGIKDDETGETEFFATEEEIYERLGYQ
                     YIPPELREDRGELDAAARGELPELIERGQIKGDLHSHTTLSDGVASLDEMAAAAEALG
                     YEYLAITDHSESHGFGNHVEADRLWQRIEEIAELNNEDRKIRLLSGSEVNIHPNGSVD
                     YEDDLLETLDWVIASIHTAFSDDAKKNTDRMITAIENPLVDCIGHPTGRLINQRDPYP
                     LDMERVIAAAVENDTLIEINSNPRRRDLNEIHARMAAEAGVGIVINTDAHRPESLDFI
                     EYGIATARRAWLTADQVRNTGPWWK"
     gene            complement(1153..>1402)
                     /locus_tag="PLE93_10070"
     CDS             complement(1153..>1402)
                     /locus_tag="PLE93_10070"
                     /inference="COORDINATES: protein motif:HMM:NF039315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HNH87439.1"
                     /translation="AFYAYAHPAEDGFAGRDLSPVPGGWNEELGEYVLDHEEAAATGN
                     PEQAVIDFCGEIFSHACSVCDWNPALAASAAGDPPPVR"
BASE COUNT          288 a          485 c          432 g          197 t
ORIGIN      
        1 cacgcaagga gctggccgcc gcgatcgccg aacacgaact ggtcgccgag cacggcaatc
       61 cgaacgagac cgggatcagg ttgaccacca actccggaat cggagtggac gtgcggatcg
      121 tcgagcccgg tgccttcggc cacctgctcc agcacttcac cggctccggc ccccataacg
      181 ccgagctccg cgaacgggca gtcgcccagg ggctgcatgt gtccgagaac gggatcaagg
      241 atgacgagac gggtgaaacg gagttcttcg cgaccgagga agagatctac gagcgcctcg
      301 gctaccagta catcccgccc gaactccgcg aggaccgggg ggaactcgac gccgccgcca
      361 ggggcgagtt gcccgagctg atcgagcggg gccagatcaa gggtgacctc cactcccaca
      421 ccaccctctc cgatggcgtc gcctcacttg acgagatggc cgcggcagcc gaagcgctcg
      481 gctacgagta cctggccatc accgaccact ccgaatccca tggcttcggc aatcacgtcg
      541 aagccgaccg gctctggcaa cggatcgaag agatcgccga gctcaacaac gaagaccgca
      601 agatccggct gctatccgga tccgaggtga acatccatcc gaacggttcg gtcgattacg
      661 aggacgacct gctcgagacc ctggactggg tgatcgcctc gatccacacc gccttcagcg
      721 atgacgcgaa gaagaacacc gaccgcatga tcaccgcgat cgagaacccg ctggtcgact
      781 gcatcggcca tccgaccggc cgcctgatca accagcgaga cccctatccc ctcgacatgg
      841 agagggtgat cgccgccgcg gtcgagaacg acaccctgat cgagatcaac tccaatcccc
      901 gccgccgcga cctgaacgag atccacgccc gcatggcggc cgaggcggga gtcgggatcg
      961 tgatcaacac cgacgcccac cggcccgaat ccctcgattt catcgagtac gggatcgcga
     1021 ccgcccgccg ggcctggctg accgccgacc aggtccggaa caccggcccc tggtggaagt
     1081 agcgaggtcg gtcaagaccc acgattttcc acaaatactg tggaaaaccg tggcttctga
     1141 tgcgaatcgg agctagcgaa ccgggggagg atcaccggcg gcgctggcgg ccagggccgg
     1201 gttccagtcg cagacgctgc aggcgtggct gaagatctcg ccgcagaagt cgatcaccgc
     1261 ctgctccggg tttccggtcg cggcggcctc ctcatggtcg agcacgtact cgcccagctc
     1321 ttcgttccac ccgcccggta ccggtgaaag gtcccggccg gcgaagccgt cctcggccgg
     1381 atgggcatac gcgtagaagg cc
//
LOCUS       DAOQFI010000639         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_68379_length_1400_cov_3.648327, whole genome shotgun sequence.
ACCESSION   DAOQFI010000639 DAOQFI010000000
VERSION     DAOQFI010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68379_length_1400_cov_3.648327"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLE93_10075"
     CDS             complement(<1..351)
                     /locus_tag="PLE93_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNH87440.1"
                     /translation="MSSENSAPRWHWFLPTSGDGRNVTNVIDQLGQTSMSRPADVEYL
                     AEVAKAAERAGFEGVLTPTGSGCEDAWVTCAAVAQHTEKLKFIVAFRPDAIAPAWAAH
                     QSASFQRLTGGRLLL"
     gene            complement(374..1306)
                     /locus_tag="PLE93_10080"
     CDS             complement(374..1306)
                     /locus_tag="PLE93_10080"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNH87441.1"
                     /translation="MSNAQFEKETDKKGAFVRQTSAFRERVTADGSSGFPAEPGRYHL
                     YVCYACPWASRTIIFRTLKGLEEVISMTAVDPVRDDQGWKFFADDPDPVNGYEYLLEA
                     YLRTDPDFSDRVTVPVLWDKETNRAVNNESSEIIRMLTSEFNQWAKNPDLDLYPEPLR
                     AEIDELNDRIYNSINNGVYRAGFATTQEAYEEAFVELFEALDVMNDRLGESRFLTGGE
                     ITEADWRFFVTLIRFDAVYVGHFKCNLRRIADYDNLAGYLRDLYQQPGIRETVNMDHI
                     KRHYYITHPRINPTQIVPMGPVLDLESDPARSHL"
BASE COUNT          232 a          413 c          482 g          273 t
ORIGIN      
        1 gagcagcagc cggccgccgg tcagccgctg gaaggaggcc gactggtgcg cggcccaggc
       61 cggcgcgatc gcatccggcc ggaaggcgac gatgaacttg agcttctcgg tgtgctgcgc
      121 gaccgcggca caggtcaccc aggcgtcctc gcaaccggaa ccggtcgggg tcagcacacc
      181 ttcgaagccg gcccgctcgg ctgccttggc aacctcggcc aggtattcga catcggccgg
      241 ccgcgacatc gaggtctgac cgagctggtc gatcacgttg gtcacgttgc gtccatcccc
      301 tgaagtggga aggaaccagt gccagcgggg cgcgctgttt tcagagctca tagtcgataa
      361 acctactcga caatcagagg tggctgcggg cggggtcact ctcgaggtcg aggacggggc
      421 ccatcgggac gatctgggtc gggttgatcc gcgggtgggt gatgtagtag tgccgcttga
      481 tgtggtccat gttgaccgtc tcgcggatgc cgggctgctg gtagaggtca cgcaggtagc
      541 cggcgaggtt gtcgtagtcg gcgatccgtc gcaggttgca cttgaagtgg ccgacgtaga
      601 cggcgtcaaa gcggatcagg gtgacgaaga agcgccagtc cgcctcggtg atctcgccgc
      661 cggtcaggaa gcggctctcc cccagccggt cgttcatcac gtcgagcgcc tcgaagagct
      721 ccacgaaggc ctcctcgtat gcctcctgcg ttgtggcgaa gccggcccgg tagaccccgt
      781 tgttgatcga gttgtagatc cggtcgttca gttcgtcgat ttccgcccgg agcggttcgg
      841 ggtaaaggtc gaggtcgggg ttcttcgccc attggttgaa ctcggaggtg agcatgcgga
      901 tgatctcgct gctctcgttg ttgaccgcgc ggttggtctc cttgtcccag agcacgggca
      961 cggtaacccg gtcgctgaag tcggggtcgg tgcgaaggta ggcctcgagc aggtactcgt
     1021 agccgttgac cgggtcggga tcgtcggcga agaacttcca gccctgatcg tcccggaccg
     1081 gatcgacggc ggtcatcgag atcacttcct ccagcccttt cagggtgcgg aagatgatcg
     1141 tccgcgaggc ccaggggcag gcgtaacaga cgtacaggtg gtacctgcca ggttccgccg
     1201 gaaagcctga agagccgtcg gcggttaccc gctccctgaa ggcgctggtc tggcggacga
     1261 acgcgccttt tttgtcggtt tccttctcaa actgtgcatt gctcacgtgg ccgactttac
     1321 ccatcggctc cggaaaacaa gccgggcgac ggattcgcgt ttatagtgtg atcagcgccg
     1381 gaaccggtcc ggctagtcga
//
LOCUS       DAOQFI010000640         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_69270_length_1391_cov_2.775449, whole genome shotgun sequence.
ACCESSION   DAOQFI010000640 DAOQFI010000000
VERSION     DAOQFI010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69270_length_1391_cov_2.775449"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLE93_10085"
     CDS             <1..729
                     /locus_tag="PLE93_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNH87442.1"
                     /translation="GSDVLAIFGLADEPRIEASSAVEGLRDRAGISEVVMLTGDSEPV
                     AAAIAKRSGITRWRAGLLPEDKLDEVIRLEREVGPTAMVGDGVNDSPALAGSTVGVAM
                     GAAGSDVALDSADVALMGDDLMRLPDAINHSRWAVRIMKQNVSVSLITKAIFVVLAPL
                     GFVSLITAIAVDMGVSLLVTLNGLRLLRRPATHSLRETPGDPDPDAVATRAEGDACEE
                     DCCSPAVPGGKAVELPIFTGNRET"
     regulatory      1007..1152
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          271 a          424 c          439 g          257 t
ORIGIN      
        1 ggatccgacg tgctggcaat cttcgggttg gcggatgagc cccggatcga agcctcgtcg
       61 gcggtcgagg gactcaggga ccgggccgga atcagcgagg tagtaatgct caccggcgac
      121 agcgagcccg tggccgcggc catcgcgaag aggagcggca tcactcgctg gcgggcaggt
      181 cttctacccg aagacaaact cgacgaggtc atacgtctcg agagggaggt cggacccacc
      241 gcaatggtcg gcgatggggt caacgactca cccgccctcg ccgggtcgac cgtcggggtc
      301 gcgatgggtg cggccggaag cgacgtcgca ctcgactcgg ccgatgtcgc cctgatgggt
      361 gacgatctca tgcgacttcc cgacgcgatc aatcactcac gatgggcggt ccggatcatg
      421 aagcagaacg tgtccgtttc cctgatcacc aaggcgatct tcgtggtcct ggcgcccctt
      481 ggcttcgtga gcctgatcac cgcaatcgcc gtcgacatgg gtgtctcact gctcgtgacc
      541 ctgaacggtc tacgtttgct gcgtcggcca gcgacccatt ccctgagaga gacccccggg
      601 gatccagatc cagacgcggt cgcgactcgc gcggaaggcg acgcatgcga agaggactgc
      661 tgttcaccgg cagtacccgg cgggaaagcg gtcgaacttc ccattttcac cggaaaccgt
      721 gaaacctaat tcctgtaact actacaggac gtcgtaggag ttgctagggt cggaatcccg
      781 ttggcctggg catcgacgct ttgaacgagc aactctccgc ttctggcctc ttggtgcgga
      841 gtagtggaca tccttgtccg ggtcaacggg atctactaca gactgtcgta gggatcgcta
      901 tgatctggat cccgccggcc cacccacaat tcggatgggc gcccccagcc gaagggccgg
      961 cgggacccca taaagggaac tgctactact tgtcgtagta tgtgagaaat ccaattagcc
     1021 gaatccggta ccaaccggat cgggggaccc acttgaggcc atcaagtctc tggggcgaat
     1081 cgcactctgc gtagcgcatc tctttcagcg cgagcccgtc agctaacccc gaaggcgcga
     1141 gaaagagaga aggaatgaat cacgattccg tcatcggacg gttggccatt agggcgttat
     1201 tgacctttgg agctctagga atgttctccg gcatcgctac cgcctcaacg ggaggtctgt
     1261 caccggcttc tccgagcccg gctccggcca agggcgcgtt cccgatccgt ggttcagtca
     1321 cctggggcga cggactagga gcgggccggg ggcatcaggg ccaggatctt ctcgccaaat
     1381 gcaccaggcc g
//
LOCUS       DAOQFI010000641         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_69457_length_1389_cov_3.278111, whole genome shotgun sequence.
ACCESSION   DAOQFI010000641 DAOQFI010000000
VERSION     DAOQFI010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69457_length_1389_cov_3.278111"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLE93_10090"
     CDS             <1..427
                     /locus_tag="PLE93_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87443.1"
                     /translation="LMPVSASAAGWGRGTFTGKVRGQFRPQPLTSVAIAARGDKVRIV
                     KLGIAFDCSDNNQFGLPLHWKTLVYSKAVRVKEGPAGGGAIIHFRVVKNYRGKKVPVR
                     GDIFLGLRDTYIRGTVDSEVARGWNPQDCSDSGIFIARR"
     gene            complement(445..>1389)
                     /locus_tag="PLE93_10095"
     CDS             complement(445..>1389)
                     /locus_tag="PLE93_10095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH87444.1"
                     /translation="PRCCSPVNGSPVASGNGRSRSFPEVTASSDLPYPPFELANRVIE
                     LPSNDFAGYVHYEMAGRQTRDELLDLLPDGYTLEGRSLLDFGCGAGRTLRQFHEEASS
                     ARVVGCDIDRESIDWMQANLAPIEAVCSEVDPPLPFESGSFDFIWAISVFTHLTGNSA
                     AWLMELHRLLKPGGLLMASYMGELNSEEVAGEPWDENRIGMNVLRHSQGWDVGGPMVL
                     MSDWWVDEHWGRAFEIRKRHFAGGQTWALLEKKDVRLSADELMAAGDDPREIAALRHN
                     VAQLQAEIDDLKSHQQGGSLARAGSALKRRASRLRRPD"
BASE COUNT          234 a          456 c          465 g          234 t
ORIGIN      
        1 cctgatgccc gtttcggctt ccgcagccgg ctggggccgc ggcaccttca ccggcaaggt
       61 gcggggtcag ttccggcctc agccgctgac cagcgtggcc attgccgcgc ggggtgacaa
      121 ggtgcggatc gtcaagctcg ggatcgcctt cgactgctcg gacaacaatc agttcggcct
      181 gccgcttcac tggaagacac tcgtctacag caaggcggtg cgggtaaagg aagggccggc
      241 cggaggcggg gcgatcatcc acttccgggt ggtcaagaac taccggggca agaaagtccc
      301 ggtcaggggc gacatcttcc tcggcctgcg cgatacctac atccggggca cggtcgattc
      361 agaggttgcc cgcggctgga atccccagga ctgctcggac agcggcatct tcatcgcccg
      421 caggtaggac gccccggcgc cggctcagtc cgggcgacgc aaacggctgg cccttcgctt
      481 cagggctgaa ccggcccggg cgagtgaccc gccctgctga tgggacttga ggtcgtcgat
      541 ctccgcctgg agctgggcga cgttgtggcg gagggcggcg atctcacggg gatcgtcgcc
      601 ggccgccatc agttcatcgg cggaaagccg gacatccttc ttttccagaa gggcccaggt
      661 ctgacctccc gcgaagtgcc gtttgcggat ctcgaaggcc cggccccagt gctcgtcaac
      721 ccaccagtcg gacatcaaga ccatcggacc gcccacatcc catccctggc tgtgacgaag
      781 cacgttcatg ccgatccggt tctcgtccca cggctcgccg gcgacctctt ccgagttgag
      841 ctcgcccatg taggaggcca tgagcagacc gcccggcttg agcaggcggt ggagttccat
      901 cagccaggcg gccgagttgc cggtcaggtg ggtgaacacc gagatcgccc agatgaagtc
      961 gaagcttccg gactcgaagg gcaggggcgg atcgacttcg gagcagaccg cctcgatcgg
     1021 cgccaggttc gcctgcatcc agtcgatgct ctcgcggtcg atgtcgcatc cgaccacccg
     1081 cgccgaggaa gcctcctcgt ggaattgccg cagggtgcgg ccggcgccgc aaccgaagtc
     1141 gagcaggctg cgaccttcca gtgtgtatcc gtcgggcagc agatccagca gctcatcccg
     1201 cgtctgccgg ccggccattt cgtagtggac gtacccggcg aagtcgttgc tgggcaactc
     1261 gattacgcgg ttggcgagct caaagggcgg ataggggagg tcgctgctcg cggtcacctc
     1321 ggggaaacta cgggatcgcc cgttcccgga ggcaaccggg ctcccgttga ccgggctgca
     1381 acatctagg
//
LOCUS       DAOQFI010000642         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_69651_length_1387_cov_3.246997, whole genome shotgun sequence.
ACCESSION   DAOQFI010000642 DAOQFI010000000
VERSION     DAOQFI010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69651_length_1387_cov_3.246997"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PLE93_10100"
     CDS             <1..1122
                     /locus_tag="PLE93_10100"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNH87445.1"
                     /translation="VAACLVEAAIFSAFDFDRYKSGSDGKEAPPSLKAVEINTGKDVA
                     AMVKVGEAVGNAGNRARYLQSLPANVATPEYLAGRAREIAEAHEKVTVEVMDRAAIIK
                     AGMGGLEAVSKGGEHVEPRLITLRYTGKGGGETLGLVGKSVTFDTGGISIKPSAGMHE
                     MKMDMSGGAAVLEGLNAIAELEIPLDIIAVLPATENMPSGTALKPGDIITQLNGKTVE
                     VTNTDAEGRLILADALVHCAREGADRLVDLATLTGAVLIGLGSTYAALISNDDDLAAA
                     VEKAGELSGELVWRLPLHDEYKDLTKGEITDLVNASAQRKAGTIYAGSFLEEFTEGKP
                     WAHLDIAGTAWDTGREYWGKGPTGFGVHLLVALAYDLSS"
     gene            complement(1129..>1387)
                     /locus_tag="PLE93_10105"
     CDS             complement(1129..>1387)
                     /locus_tag="PLE93_10105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNH87446.1"
                     /translation="GFMATLLDSVAGCAVHTTLALGEAYTTLTLEVKNLRALTRDTGS
                     VRAEGEVTYRGRRQATADAKLYAEESGKLLASATSTCLIMA"
BASE COUNT          245 a          481 c          464 g          197 t
ORIGIN      
        1 gtcgccgcct gcctggtcga agcggcgatc ttcagcgcct ttgacttcga ccgctacaag
       61 tcgggaagtg acggcaagga ggccccgccc tcactgaagg ccgtcgagat caacaccggc
      121 aaggacgtgg ccgcgatggt caaggtcggg gaagccgtcg gcaacgcggg aaaccgggcc
      181 cgctacctgc agagcctgcc ggccaacgtg gcgacccccg agtacctggc cggccgggcc
      241 cgcgagatcg ccgaagcgca cgagaaggta acggtcgaag taatggaccg cgcggcgatc
      301 atcaaggccg gcatgggtgg cctcgaggcc gtctccaagg gcggcgagca cgtcgagccg
      361 cgcctgatca ctctccgtta caccggcaag ggcgggggcg agacgctcgg cctggtcggc
      421 aagtcggtca ccttcgacac cggcggcatc tcgatcaagc cctcggccgg aatgcacgag
      481 atgaagatgg acatgtccgg cggcgccgcg gtgctcgagg gcctgaacgc gattgccgag
      541 ctggagattc cgctcgacat catcgccgtc ctcccggcga ccgagaacat gccgtccgga
      601 accgccctga agcccggcga catcatcacc cagctcaatg gcaagacggt cgaggtgacc
      661 aacaccgacg ccgagggccg gctgatcctg gccgatgccc tggtccactg cgcccgcgaa
      721 ggcgccgacc gcctggtcga ccttgcgacg ctgaccggcg cggtgctgat cggcctcggc
      781 tcgacctacg cggccctgat ctcgaatgac gacgacctcg ccgcagccgt cgagaaggcg
      841 ggcgagctga gtggcgaact ggtctggcgc ctgccgctgc acgacgagta caaggacctg
      901 accaagggcg agatcaccga tctggtcaac gcctccgccc agcgcaaggc cggcacgatc
      961 tacgccggat cattcctcga ggagttcacc gagggcaagc cctgggccca cctggacatc
     1021 gccggcaccg cctgggacac cggccgcgag tactggggca agggcccgac cggtttcggc
     1081 gttcacctgc tggtcgccct cgcgtacgac ctcagcagct gatccgggtc acgccatgat
     1141 cagacaagtc gaggtggctg acgcgagcag cttgccgctt tcctcggcgt agagcttcgc
     1201 gtcggcggtc gcctgccgcc ggccccggta ggtgacctca ccctcggccc gcacgctgcc
     1261 cgtgtcgcgg gtcagcgcac gcaggttctt cacttccagg gtgagagtcg tgtaggcctc
     1321 gcccagcgcc agcgtcgtgt gaactgcgca acccgcgact gaatcgagca gggtcgccat
     1381 gaagccg
//
LOCUS       DAOQFI010000643         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_69669_length_1387_cov_2.747748, whole genome shotgun sequence.
ACCESSION   DAOQFI010000643 DAOQFI010000000
VERSION     DAOQFI010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69669_length_1387_cov_2.747748"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..568
                     /locus_tag="PLE93_10110"
     CDS             113..568
                     /locus_tag="PLE93_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNH87447.1"
                     /translation="MIINNKDATVDAMVGGDDYDTTPFNLATQGYRQPGSTIKPFILT
                     TALGQGISPYTTFESAPQVFWRKWKIKDKKTGKWKWHREPYSVSNYGDSYAGVSDLVS
                     ATINSDNSVYAQLGIEAIDGGPKAIASMPSWATPTRSKPTRRWCSARPK"
     gene            544..1314
                     /locus_tag="PLE93_10115"
     CDS             544..1314
                     /locus_tag="PLE93_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNH87448.1"
                     /translation="MVLGTSEVTPLGWTYAFSTLANDGRRVSGTLAPDPGDDPVSFTK
                     ITDEDGKTIKGGDNEVLSKQVIDPDVASEAKTILHEVVTQGTGKNAYIGDDSQWGKTG
                     TTEDNTNAWFCGAITEITACVWVGYPEGYVQMTTEYGGAPVDGGTFPAIIWSRVVSAW
                     KEIEANRAAERAADAADGDSSDSSDVTTDSSDYSYTAPSTDYTTPTYTAPETTTPVAP
                     APTPTTTAPAPTTTVPTTPTTPSTGGGVAPSSGGASPG"
BASE COUNT          290 a          492 c          401 g          204 t
ORIGIN      
        1 aataatcccc accagctttt ctttgatctg atccgcggtg aacggtttgc gcacataacc
       61 ggtcgccccc agattgaccg cctccatcac cttgttctga ctcccctcgg tcgtgatcat
      121 caacaacaag gacgccacgg ttgacgcgat ggtcggcggc gacgattacg acaccacgcc
      181 cttcaacctc gcgacccagg ggtacaggca gcccggctcg acgatcaagc ccttcatcct
      241 caccaccgcc ctcgggcagg gcatctcgcc ctacacgact ttcgaatcgg caccccaggt
      301 cttctggcgc aagtggaaga tcaaggacaa gaagaccggc aagtggaagt ggcatcgcga
      361 gccctattcg gtcagcaact acggcgactc ctatgccggc gtaagcgacc tggtcagcgc
      421 gaccatcaac tccgacaact cggtctacgc ccagctcggc atcgaagcga tcgacggcgg
      481 ccccaaggcg atcgcttcga tgccgagctg ggctactccg acaagatcga aaccaacccg
      541 gcgatggtgc tcggcacgtc cgaagtgact ccgctcggct ggacctatgc cttctcgacc
      601 ctggccaatg acggccgccg ggtcagcggc accctcgcgc cggacccggg agacgacccg
      661 gtcagcttca ccaagatcac cgacgaggac ggcaagacga tcaagggcgg tgacaacgag
      721 gtgctcagca agcaggtgat cgacccggat gtcgccagcg aagccaagac catcctgcat
      781 gaggttgtca cccagggcac cggcaagaac gcctacatcg gcgatgacag ccagtgggga
      841 aagaccggca cgaccgaaga caacacgaac gcatggttct gtggcgcgat caccgagatc
      901 accgcctgcg tctgggtggg ttaccccgaa ggctatgtac agatgaccac cgagtacggc
      961 ggcgccccgg ttgacggtgg caccttcccg gcgatcatct ggtcccgggt ggtttcggcc
     1021 tggaaggaaa tcgaagccaa ccgtgcggcc gaacgggcag cggatgccgc cgacggcgac
     1081 agcagcgact catccgacgt gaccaccgac tcgagcgatt acagctacac cgcacccagc
     1141 accgactaca ccacgccgac ttacacggcc ccggagacga caacccccgt cgctccggcc
     1201 ccgacaccga cgacaacggc tcccgcaccg actaccacgg tgcccaccac gccgaccacg
     1261 ccttccacgg gcggtggcgt cgcaccctca tccggcggcg cttcccccgg ttagccgggt
     1321 cagcgcacgg cctcagccga tttcgcggag ccgggccttg gccaggtcga tctgggcggc
     1381 gaggctt
//
LOCUS       DAOQFI010000644         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_69674_length_1387_cov_2.683183, whole genome shotgun sequence.
ACCESSION   DAOQFI010000644 DAOQFI010000000
VERSION     DAOQFI010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69674_length_1387_cov_2.683183"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLE93_10120"
     CDS             complement(<1..278)
                     /locus_tag="PLE93_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87449.1"
                     /translation="MSQITKPTETIQLPAPSFAPAVFALGVLGLIAGTFANDFMFPAW
                     FYAFVGFFFCLFSLRSMIKKGRRAFYALPREQGDARAELPVESFTAPTA"
     gene            complement(487..>1387)
                     /locus_tag="PLE93_10125"
     CDS             complement(487..>1387)
                     /locus_tag="PLE93_10125"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNH87450.1"
                     /translation="AQPAQSGLQLDLIPMVRDYISLTKPRIISLLLLTTVATMFVADP
                     SGPALSTILWTMLGGYLAAGGAGAINHYIDRNRDARNNRTKDRPLADGRIKPINGLIF
                     GIVLGVISFFQLWLTVNLLAAGLAMVGLFGYVFLYTLWLKPLTPQNIVIGGSAGAVPP
                     LVGWAAASGSLTWEAMWPFLIIFFWTPPHFWALSLMIKDDYAKTGVPMLPVVKGEEAT
                     RQSILIYTIFMVGFTVGPYCTGLVGPFYLAASVVLGLIYIGFSFMLWRRPSPKITLQS
                     YLYSLAYLALLFISMAVAAVASA"
BASE COUNT          289 a          382 c          508 g          208 t
ORIGIN      
        1 gcggtggggg cggtgaagct ctcgaccggg agttcggccc gggcgtcacc ctgctcgcgg
       61 ggcagggcgt agaaggcgcg gcggcccttc ttgatcatgc tgcgcagcga gaagaggcag
      121 aagaagaagc cgacgaaggc gtagaaccag gccgggaaca tgaagtcgtt ggcgaaggtg
      181 ccggcgatca ggccgagcac gccgagggcg aagactgccg gggcgaagga gggagcggga
      241 agctggatcg tttcggtcgg cttggtgatc tggctcatga gttgtcccta gttcgcgatg
      301 tagaggaccg caaagacgaa agtgagggcg aagatggacg cggaagtcgc gccgagcagc
      361 atgcccgtgg tgaagttttt cttggaagtg ttgcgatcca tgggacctat ttctatcaat
      421 tcggggggcg ttccggtcgc ctgaagcgga gtcccgccgg cgaagaccgg cgcgactgcg
      481 gtcaggtcac gcggaagcca cggctgccac ggccatgctg atgaaaagca gggcgaggta
      541 ggccagcgag tagaggtagg actggagcgt gatcttcggg ctcggccggc gccagagcat
      601 gaacgagaag ccgatgtaga tcaggccgag aaccaccgag gcggccaggt agaaggggcc
      661 aaccagcccc gtgcagtagg gaccgaccgt gaatccgacc atgaagatcg tgtagatcag
      721 gatcgactgg cgggtcgctt cctcgccctt gaccaccggc agcatcggca cgccggtctt
      781 cgcataatcg tccttgatca tcagcgagag tgcccagaaa tggggcgggg tccagaagaa
      841 gatgatcagg aagggccaca tcgcctccca ggtcagggaa cccgaggcgg cggcccagcc
      901 gaccagcggc ggaacggccc cggcgctgcc accgatcacg atgttctgcg gggtcagcgg
      961 cttcagccag agcgtgtaga gaaagacgta cccgaacaga ccgaccatcg ccaggccggc
     1021 tgcgagcagg ttgaccgtga gccagagctg gaagaaggaa atgaccccga ggacgatgcc
     1081 gaagatcagc ccgttgatcg gcttgatccg accgtcggcc aggggccggt ccttggtccg
     1141 gttgttgcgc gcgtcgcggt tgcggtcgat gtagtggttg atcgctccgg cgccaccggc
     1201 cgccagataa ccgccgagca tggtccagag aatggtggag agggcggggc cggatgggtc
     1261 agccacgaac atcgtcgcca ccgtggtcaa cagcagcagg ctgatgatcc gcggtttggt
     1321 cagcgagatg taatcccgaa ccatcgggat cagatcaagc tgcaggccgg actgcgcggg
     1381 ctgcgcg
//
LOCUS       DAOQFI010000645         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_69676_length_1387_cov_2.613363, whole genome shotgun sequence.
ACCESSION   DAOQFI010000645 DAOQFI010000000
VERSION     DAOQFI010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69676_length_1387_cov_2.613363"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLE93_10130"
     CDS             complement(<1..543)
                     /locus_tag="PLE93_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87451.1"
                     /translation="MPETVSGESSANVSAFETQEPTHRVLLVADEVFRGSDLADELAA
                     HGTDRGSIGVFVISPAMTHSLIDQELGNVDQTVIEASDRLETILAELRKVGISARGEV
                     GDADPMVAIGDGVREFEPDQIMVVTHVDSEREYAEKNLWDRLGTDFHAPVTQLKVPHP
                     AGEGGIPAVESVEHFSAHQRT"
     gene            complement(670..>1387)
                     /locus_tag="PLE93_10135"
     CDS             complement(670..>1387)
                     /locus_tag="PLE93_10135"
                     /inference="COORDINATES: protein motif:HMM:NF026214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HNH87452.1"
                     /translation="KSTYTRYLGWYDGNPANLEPLVPVESGKRYVEFMGGADNVVSKA
                     RESFEKGEYRWVAEVLGHVVFADPANQEARELQADAFEQLGYQTESSTWRNAYLMGAQ
                     ELRHGTPSFPIGGTASEDSIRAMTLPMIFNFFAIKIHGVDAADLELDVNLDITDTGES
                     AVLRLSNGVLSHSMTDRDEDAPVLEMSRELLNKLVGNEITLEEAVEAGEVESGPGLEV
                     FRKFGEHLDEFDLWFPIIEP"
BASE COUNT          240 a          444 c          421 g          282 t
ORIGIN      
        1 ggttctctga tgggccgaga aatgctcaac cgactcgacc gcggggatcc cgccctcgcc
       61 agccgggtgg ggaaccttca gctgggtgac cggagcatga aagtcggtcc cgagccggtc
      121 ccagagattc ttctcggcat actcccgctc actgtcgacg tgggtgacca ccatgatctg
      181 gtcgggctcg aattcccgta ctccgtctcc gatcgccacc atcgggtcgg cgtcaccgac
      241 ctcgccacgg gccgaaatgc cgaccttgcg cagttcggcc aggatcgtct caaggcggtc
      301 cgacgcttcg atcaccgtct ggtcgacgtt tccgagttcc tggtcgatca gggaatgggt
      361 catcgcgggt gagataacga agaccccgat cgagccccga tcggttccgt gggctgccag
      421 ctcgtcggcc aggtcacttc cgcggaagac ctcatcggca accagcagca cccggtgagt
      481 tggttcctgg gtttcgaacg ccgaaacgtt cgcggatgat tcaccactaa cggtctcagg
      541 catattttca acctacccgg cttcaggcaa ccggaaccgc tttttacgga atttcccgga
      601 gcaccgggct gagcggcctc gggaaagcag cggcgaaggc gggatttttc gtctcgcctt
      661 cgattccgtt tacggctcga tgatcgggaa ccagaggtcg aactcgtcca gatgctcgcc
      721 gaacttgcgg aagacctcga gaccgggacc cgactcgact tcgcccgctt cgaccgcttc
      781 ctcgagcgtg atttcgtttc cgaccagctt gttgaggagc tcccgggaca tctcgagcac
      841 cggcgcatcc tcatcccggt cggtcatcga atggctgagc acgccgttcg agagcctcag
      901 caccgcggat tccccggtgt cggtgatgtc caggttcacg tccagttcga ggtcggccgc
      961 gtcgacgccg tgaatcttga tcgcgaagaa gttgaagatc atcggcagcg tcatcgcccg
     1021 gattgaatcc tcgctggccg tgccaccgat cgggaagctc ggagtgccgt gccggagttc
     1081 ctgggcgccc atcagatagg cgtttcgcca ggtcgatgat tcggtctggt agccaagctg
     1141 ctcgaaggcg tccgcctgca gttcgcgggc ctcctgattg gcaggatcgg cgaagacgac
     1201 gtgtccgagc acttccgcga cccagcggta ctcgcccttt tcgaatgatt cccgggcctt
     1261 gctgaccaca ttgtcggccc cgcccatgaa ctcgacgtag cgctttccgg attccaccgg
     1321 aaccagcggc tcgaggttgg ccgggttgcc gtcataccag ccgaggtacc gggtgtaggt
     1381 cgacttg
//
LOCUS       DAOQFI010000646         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_69708_length_1387_cov_2.074324, whole genome shotgun sequence.
ACCESSION   DAOQFI010000646 DAOQFI010000000
VERSION     DAOQFI010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69708_length_1387_cov_2.074324"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1036)
                     /locus_tag="PLE93_10140"
     CDS             complement(29..1036)
                     /locus_tag="PLE93_10140"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNH87453.1"
                     /translation="MTQHKMPLLDQIVNLFAVVIPFLATIAAIVLFWNQIVTPLDLAL
                     FLGMYMLTTFGITIGFHRLLTHRSFQTFKPIEYFLAIAGSMAVEGPVNTWVADHRKHH
                     AHTDEEGDPHSPHVGHGGGIKGVLKGLFHAHIGWTFTDHGKAEQEKYAKDLYEDRGMR
                     FISDFFVLWVVLGLLIPAGIGYLVTGTLAGAASGFLWGGLVRVFFVHHVTFSINSVCH
                     FLGTRRFEVDDYSTNVFWLALPSMGEAWHHNHHAFPRSANHGLKKWELDPSAWVITAM
                     EKTGLARNVVRISPERQEQKLMKSHGSEPSTQAGPGSGVPASPTRNVVSRQTEPGPPV
                     T"
     gene            1171..>1387
                     /locus_tag="PLE93_10145"
     CDS             1171..>1387
                     /locus_tag="PLE93_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87454.1"
                     /translation="MIEPELSGIADEILVSIGQLIPEYAMPLEGSFGKAVYRGVGEAL
                     DQFLLLIRDPDADRRASRKVYPILGRGE"
BASE COUNT          248 a          412 c          471 g          256 t
ORIGIN      
        1 cctgatccga tggccggagc gctagcccct aggtgacggg aggaccgggt tcggtctggc
       61 ggctgacgac gtttcgggtc ggggaagccg gtacaccgga ccccggcccc gcctgagtcg
      121 acggttcaga tccgtgcgac ttcatcagct tctgttcctg gcgttcgggg gagatgcgga
      181 cgacgtttct ggcgagtccg gtcttttcca ttgccgtgat cacccaggcg ctggggtcca
      241 gctcccactt cttcaggccg tggttggccg agcggggaaa ggcgtgatgg ttgtggtgcc
      301 aggcctcgcc cattgatggg agcgccagcc agaagacgtt ggtcgagtag tcgtcgacct
      361 cgaagcgccg cgtgcccagg aagtggcaga ccgagttgat cgagaaggtg acgtggtgga
      421 cgaagaagac gcggaccagc ccgccccaga ggaagccgct cgcggcgccg gccagggtgc
      481 cggtcaccag gtaaccgatc ccggccggga tcagcaggcc aaggaccacc cagagcacga
      541 agaagtcact gatgaacctc atgccgcggt cctcgtagag atccttcgcg tacttctcct
      601 gctcggcctt gccgtggtcg gtgaaggtcc agccgatatg cgcgtggaag aggcccttca
      661 gcacgccctt gatgccgccg ccgtggccga catgggggct gtgcggatca ccctcctcgt
      721 cggtgtgggc gtgatgcttg cgatggtcgg cgacccaggt attgaccggg ccctccaccg
      781 ccatcgaccc ggcgatcgcg aggaagtatt cgatcggctt gaaggtctgg aacgagcggt
      841 gggtgagcag ccggtggaag ccgatcgtga tgccgaaggt cgtgagcatg tacatgccga
      901 ggaacagggc gaggtcgagc ggggtcacga tctggttcca gaacaggacg atcgccgcga
      961 tcgtcgccag aaacgggatg accactgcga acaggttcac gatctggtcc agcaggggca
     1021 tcttgtgctg cgtcatgcgc ggaaatctat agtcgaaaac ctagtatttc ttcttgttct
     1081 gcgaaggtat ctgaagcagt tgttataaat cttcttataa gaactgcgcg ccaagagccc
     1141 tggcgggacc tcgatgccga ggttgccgac gtgatcgaac cggagctttc cgggatcgcc
     1201 gacgagatcc tcgtttcgat cgggcagctg atccccgagt acgcgatgcc gctcgaaggc
     1261 tcgttcggca aggccgtcta tcgcggcgtc ggcgaggccc tcgaccagtt cctgctcctg
     1321 atccgcgacc cggatgccga ccggcgggcc agccgcaagg tctacccgat cctcggccgg
     1381 ggcgagc
//
LOCUS       DAOQFI010000647         1383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_70081_length_1383_cov_2.526355, whole genome shotgun sequence.
ACCESSION   DAOQFI010000647 DAOQFI010000000
VERSION     DAOQFI010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70081_length_1383_cov_2.526355"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLE93_10150"
     CDS             complement(<1..274)
                     /locus_tag="PLE93_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87455.1"
                     /translation="MNSQSEILEREKRWSLIAGIASVGAVALFFIGQIVGRSGVGSPE
                     GTADFLAALSGHHSTAMLGAVIQGIATVLLAVPLAYLFFAALGRSDR"
     gene            complement(311..484)
                     /locus_tag="PLE93_10155"
     CDS             complement(311..484)
                     /locus_tag="PLE93_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87456.1"
                     /translation="MSSSDSDPGRIESLIVAVSPLLDLVLAAGERISRIAEPVDYEYY
                     PVRDKDEEERPGE"
     gene            507..1208
                     /locus_tag="PLE93_10160"
     CDS             507..1208
                     /locus_tag="PLE93_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87457.1"
                     /translation="MTIDLPHVPLEAGSGPENPPCPACGEPLFPWVGMPVGSGIAHRC
                     EACGLGVLSHGEKFSFPPPVGMEGATDRGKGPDSFDPGTSVDALRELDSERDEDGSYL
                     FENRASLVCWVTGGAWVGLGTDRRYRFTPAAVTDLIASRDQVVTKVRWRPLRGIAVTW
                     QSGLNMFTFGQNTVLGSLGKVFGVPADRPWKRGLDWFISVAVAIPAIIVAVPLELIGI
                     LFRRGASARAEVQVL"
BASE COUNT          215 a          456 c          483 g          229 t
ORIGIN      
        1 tccggtcgga tcggcccagc gccgcgaaga acaggtaggc gagcgggacc gcgagcagga
       61 cggtggcgat gccctggatg accgcgccca gcatcgcggt cgagtgatga ccggacaggg
      121 cggccaggaa atcggccgtc ccctcgggcg atccgactcc ggaccggccg acgatctgcc
      181 cgatgaagaa gagggcgacg gctccgaccg aagcgatgcc cgcgatcagg gaccagcgct
      241 tctcgcgctc gagtatctcg ctctgtgaat tcatgggtgg gcccgaaggc cgggcaagcc
      301 tacaggccgg tcactccccg gggcgttcct cttcgtcctt atcccggacc gggtagtact
      361 cgtaatcgac cggttcggcg atccgcgaga ttcgctcacc ggccgcgagg accagatcca
      421 gcagcggcga tacggcgacg atcagcgatt cgatcctgcc ggggtcggag tcggatgaac
      481 tcatggggca atacgctaca gcgagtttga cgatcgatct gccccacgtt cccctcgaag
      541 ccggatccgg tccggaaaat cctccctgcc cggcctgcgg cgagccgctc ttcccctggg
      601 tcggcatgcc ggtcggctcc gggatcgccc accgctgcga ggcctgcggg ctcggtgtcc
      661 tcagccacgg tgagaagttc agctttccgc cgccggtcgg aatggaggga gccacggatc
      721 gtggcaaggg gcccgattcg tttgatcccg gaacctcggt cgacgcgctt cgtgagctgg
      781 attcggaacg ggacgaggat ggttcctacc tctttgagaa ccgggcctcg ctggtttgct
      841 gggttaccgg aggggcgtgg gtcggactcg ggaccgatcg tcgctaccgg ttcactccgg
      901 ctgcggtgac cgacctgatt gcgagccgcg accaggtcgt gaccaaggtg cgctggcggc
      961 cgctgcgggg catcgcggtc acctggcagt cgggactgaa catgttcacc ttcggccaga
     1021 acaccgtgct cggctcgctg ggcaaggtct tcggcgttcc ggccgaccgg ccctggaagc
     1081 gcgggctcga ctggttcatc tcggtcgcgg ttgcgatccc ggcgatcatc gtcgccgtcc
     1141 cgctcgagct gatcgggatc ctctttcgcc gcggcgcctc ggcccgggcc gaagtccagg
     1201 tcctctaggc gagcaaggcc gcaacggcgg cgatgccgtc ccggctggaa tgaatggtcg
     1261 ccgctgccgg cacacggtcg gcgacagcct gcgaggcccg gccgagcagg tccggggttg
     1321 acggcagggt aacgacggtc acctccgatt cgagtccggc cagccggacc ggatccaggg
     1381 acc
//
LOCUS       DAOQFI010000648         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_70532_length_1378_cov_3.076342, whole genome shotgun sequence.
ACCESSION   DAOQFI010000648 DAOQFI010000000
VERSION     DAOQFI010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70532_length_1378_cov_3.076342"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="lysS"
                     /locus_tag="PLE93_10165"
     CDS             <1..1138
                     /gene="lysS"
                     /locus_tag="PLE93_10165"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNH87458.1"
                     /translation="VIFCATITFRKVLTQLSLEVTSWQVLTKALRPPPDKFHGLEDTE
                     TRYRRRELDLIANEESREIFRVRARTVSEVRRWLDDHGFFEVETPVLQPLYGGALARP
                     FVTHHNALDRQLFLRIATELYLKRCVVGGIDKVYELGKDFRNEGISMKHNPEFTMLEW
                     YEAYADYSDTAAELEELVSGVAEKVLGKAEIERDGRTISLAAPWHRVTLRDAILEHSG
                     IDIDELTDRDALSEAIGNEDETAGWGKLVDTVFSKRVEPRLVEPTFILDYPEELSPFA
                     KQHRDKPGKVERWEAFIGGVEIANSFTELNDPDEQRRRFEQQAAEIDRGDEEAQPFDE
                     SFIEALEQGMPPTGGVGLGIDRLVMMLTGAGSLREVVLFPAMRD"
     gene            complement(1169..>1378)
                     /locus_tag="PLE93_10170"
     CDS             complement(1169..>1378)
                     /locus_tag="PLE93_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87459.1"
                     /translation="GRGRNAGKGQLVFDYAAADNTGTYCYQSSKLFMVPRGDYRRGIR
                     QLIRIYHSAGYFGPGLPKGPNGEPA"
BASE COUNT          244 a          460 c          444 g          230 t
ORIGIN      
        1 tgttatcttt tgcgccacca tcaccttcag gaaggttctc actcagcttt cccttgaagt
       61 cacctcctgg caggtcctga ccaaggccct caggccgccc ccggacaagt tccacgggct
      121 cgaggacacc gagacccggt atcgccgccg cgaactcgac ctgattgcca acgaagagtc
      181 acgcgagatc ttccgcgtcc gcgcccgcac cgtgagcgag gtccgccgct ggctcgacga
      241 ccacggattc ttcgaggtcg agacaccggt cctccagccg ctttacggcg gtgccctggc
      301 ccggcctttc gtgacccatc acaacgctct cgaccgtcag ctttttctgc gcatcgcgac
      361 cgagctctac ctcaagcgtt gcgtggtcgg cggcatcgac aaggtctatg aactgggcaa
      421 ggacttccgc aacgagggca tctcgatgaa gcacaacccc gagttcacga tgctcgagtg
      481 gtacgaggcc tacgcggatt acagcgacac cgcggccgag ctcgaggaac tggtctccgg
      541 ggtcgccgaa aaggtgctcg gcaaggcgga gatcgagcgg gacggccgaa cgatcagcct
      601 cgccgcgcca tggcaccggg tcaccctccg cgacgcgatc ctcgagcatt cggggattga
      661 catcgacgag ctgaccgatc gggatgccct ttcggaggcg atcggcaacg aagacgagac
      721 cgccggctgg ggcaagctcg tggacacggt cttctcgaaa cgggtcgagc cccggctggt
      781 cgaacccacc ttcatcctcg attacccgga agagctttct cccttcgcca agcagcaccg
      841 ggacaagccg gggaaggtcg agcgctggga ggccttcatc ggcggtgtcg agatcgccaa
      901 ttcgttcacc gagctgaacg acccggacga gcagcggcgc cgcttcgaac agcaggcggc
      961 cgagatcgac cggggcgacg aagaggccca gcccttcgac gagtcattca tcgaggcgct
     1021 ggaacagggc atgccgccga ccggcggtgt cgggctcggc atcgaccgcc tggtgatgat
     1081 gctgaccggt gccggctccc tccgggaagt ggttctcttc ccggcgatgc gggactgatc
     1141 gaccggccgc ggggcgtggc cccgaccctc aggccggttc gccgttcggg cccttgggca
     1201 ggcccggtcc gaagtagccg gccgaatggt agatccggat cagctgcctg atgccgcgcc
     1261 ggtagtcgcc acgcggaacc atgaagagct tggagctctg gtagcagtag gtgccggtgt
     1321 tgtcggcggc ggcgtaatcg aagaccagct ggcccttgcc ggcgttccgt ccccggcc
//
LOCUS       DAOQFI010000649         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_70831_length_1375_cov_4.009091, whole genome shotgun sequence.
ACCESSION   DAOQFI010000649 DAOQFI010000000
VERSION     DAOQFI010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70831_length_1375_cov_4.009091"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PLE93_10175"
     CDS             complement(<1..898)
                     /locus_tag="PLE93_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNH87460.1"
                     /translation="MGKGSFMLVLLVGLTIAFGSATSAASLPRLKADPDTARGGRIVD
                     TKGREVILRGVNVNSLGQYWQGTSKPPTLPLEKKDPARIAAIGWNLVRLIVSWSRVEP
                     RPGVINTAYLDRVARWTGLMEEEGIYVMLDFHQDAWGPTLAAPDGQVCTPPAQPAFGW
                     DGAPGWATLDDGQPRCFTSNREINPAVRAAWTNFFDDKPASDGVGIQTHYVRMLKRVA
                     KRFAKDPGVAGIDIMNEPNAFGPVQTEQLGRFYGRSLKSIRAGEKAGGGFRHLVFFEP
                     GVLWSLLGSGAPGPFNFDSNVVY"
     gene            998..>1375
                     /locus_tag="PLE93_10180"
     CDS             998..>1375
                     /locus_tag="PLE93_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87461.1"
                     /translation="MDRKVGAALAVAIIGVIVGVVGIVMAKDAKDANQDTQAQLEQQI
                     KQSNKSSAAKVAAAAAAAKVIENKAASKAAESEKQDESSLKSAESKDSSQASANASDI
                     QTLEDKVDELSTEIGNVSGQLKSN"
BASE COUNT          271 a          447 c          457 g          200 t
ORIGIN      
        1 aatagacgac gttcgagtcg aagttaaagg ggccgggagc gccgctgccg agcagcgacc
       61 agagcacccc cggttcgaag aagacgagat gccggaagcc gccgcccgcc ttctcgccgg
      121 cccggatcga cttcagcgaa cgaccgtaga accggccgag ctgttcggtc tggaccgggc
      181 cgaaggcgtt gggctcgttc atgatgtcga tcccggcgac gccagggtcc ttcgcgaacc
      241 gcttcgctac ccgctttagc atccgcacgt agtgggtctg gatcccgacc ccgtccgagg
      301 ccggcttgtc atcgaagaaa ttggtccagg cggcgcgcac ggccggattg atctcgcggt
      361 tgctggtgaa gcagcggggc tggccgtcat cgagagtcgc ccagcccggg gcgccgtccc
      421 agccgaaggc aggctgagcc gggggagtgc agacctggcc atcgggggcc gccagggtcg
      481 gcccccaggc atcctgatga aaatcgagca tcacgtagat gccctcctct tccatcaggc
      541 cggtccagcg cgcgacccgg tccaggtagg cggtgttgat cacccccggc ctcggttcga
      601 cccgcgacca ggagacgatc aaccggacca ggttccatcc gatcgcggcg atccgggcgg
      661 ggtccttctt ctcgagcggc agggtgggcg gcttcgaggt gccctgccag tactggccga
      721 gggaattcac attgaccccg cggaggatca cctcccggcc cttggtgtca acgatccggc
      781 caccgcgcgc ggtgtcggga tcggccttga gccgcggcag gctggccgcc gaggttgcgg
      841 atccgaacgc gatcgtcagg ccgaccagca gcaccagcat gaaactcccc ttgcccatcg
      901 gcccagtgta gtccagccgt caggtccggc tgtcacagtc ccggcagcca gtccaaactc
      961 gcattccggc ggtcagtcct ccggggaagg aaacgaaatg gatcgcaaag tcggagcagc
     1021 cctggccgtc gcaatcatcg gcgtgatcgt cggtgtggtc gggatcgtca tggccaagga
     1081 cgccaaggac gcaaaccagg acacccaggc tcagctcgaa cagcagatca agcagagcaa
     1141 caagagctcg gcggccaagg tcgccgcggc cgcagcggca gcgaaggtga tcgagaacaa
     1201 ggccgccagc aaggcagccg agtccgagaa gcaggacgag agttcgctga agtcagccga
     1261 gtcgaaggat tcgtcccagg cgtcagccaa cgcctcggac attcagaccc tcgaagacaa
     1321 ggttgacgag ctgagtaccg agatcggcaa cgtctcgggg cagctcaaga gcaac
//
LOCUS       DAOQFI010000650         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_70933_length_1374_cov_3.549659, whole genome shotgun sequence.
ACCESSION   DAOQFI010000650 DAOQFI010000000
VERSION     DAOQFI010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70933_length_1374_cov_3.549659"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1014
                     /gene="lipB"
                     /locus_tag="PLE93_10185"
     CDS             211..1014
                     /gene="lipB"
                     /locus_tag="PLE93_10185"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNH87462.1"
                     /translation="MAALTVIELGRRDYAGMVEVQERLAADRLAGSIGDLLLLLEHPP
                     TYTLGRRSEPSDLLHGAGWYRERGIEVSRTPRGGKVTYHGPGQLIAYPIVDLRGVGEQ
                     PAGADRVDVARFVASLEGAMADSLWELGIPAGRIEGLTGLWVDDERRDPGDFTDADAA
                     SAAAGLARGSIRKIGSIGLKIRRGVTSHGLSLNVSCDLEPFDWINSCGIETCRATSIA
                     VELGGEAPSVESVGKAVVASLASRLGLEPTTGNPADFGLAEANPANSVA"
     gene            1135..1248
                     /locus_tag="PLE93_10190"
                     /pseudo
     CDS             1135..1248
                     /locus_tag="PLE93_10190"
                     /inference="COORDINATES: protein motif:HMM:NF028191.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            1231..1374
                     /locus_tag="PLE93_10195"
                     /pseudo
     CDS             1231..1374
                     /locus_tag="PLE93_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
BASE COUNT          205 a          485 c          463 g          221 t
ORIGIN      
        1 ctccgcgatc gccttcagct tcgccgttct cggctcctcc gttctggtgg cctgtgcctt
       61 ttgcgtcggg tgctttcgtt gccgccgctg cgctttctgc gtcggctgca cgaactgcgt
      121 cgactgcgtt ggctgtgtcg gttgccgggg actcaccggg gcccgtggcg tcgtcggcaa
      181 gtcggatgag gagcagaaag gaatccggcg gtggctgccc tcacggtgat cgaactcggc
      241 cggcgcgatt acgccggaat ggtcgaagtc caggaacggc tggccgccga ccggctcgcc
      301 ggctcgatcg gtgatctcct tctgctgctc gaacatccgc ccacctacac cctgggccgc
      361 cgctccgaac cgtcggacct gcttcacgga gccgggtggt accgcgagcg cggcatcgaa
      421 gtcagccgga caccgcgggg cggcaaggtc acctaccacg gcccgggcca gctgatcgcc
      481 tatccgatcg tcgacctgcg gggggttggg gagcagcccg ccggggccga ccgggtcgac
      541 gtggcccgct tcgtcgcctc actcgaaggc gcgatggccg acagcctctg ggagctggga
      601 atcccggcgg gccggattga gggcctgacc gggctctggg ttgacgatga acgccgggac
      661 ccgggggact tcaccgacgc cgacgccgcg tcggccgccg cggggctggc ccgcggcagc
      721 atccgcaaga tcggctcgat cgggctgaag atccggcggg gcgtgaccag ccatggcctt
      781 tcgttgaacg tttcctgcga tctcgagccg tttgactgga tcaactcctg cggcatcgag
      841 acctgccggg cgacctcgat cgcggtcgag ctcggtggcg aggcaccttc ggtcgagtcg
      901 gtcggaaaag ccgtggtggc cagtctggcc tcgcgcctcg gactggagcc aaccaccggc
      961 aatcctgccg acttcggcct ggccgaagcg aatcccgcca attccgtagc ctaggtggcg
     1021 gtgagcaccc cgccccccga cagcgcgacc ctcgaaggca gccccgccga gcggatccac
     1081 gtgacccgct cccgggcgaa tcccggcggc ggcaccgtcc agcccgatga cgcggtgccg
     1141 ttccgttccc gcaagccttc ctggctgaaa gtaccgccgc ccggcggccc ggtctaccgc
     1201 gagatcaagc agatgatcga tggcgagagc ctgaacacgg tctgcgagga agcgaactgc
     1261 ccgaacatcg gcgagtgctg ggaacgcggc accgccacct tcatgatcct cggcgacgtc
     1321 tgcacccgcc gctgcggctt ctgcaacgtc cagaccggca agccaaccca caac
//
LOCUS       DAOQFI010000651         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_70939_length_1374_cov_3.307051, whole genome shotgun sequence.
ACCESSION   DAOQFI010000651 DAOQFI010000000
VERSION     DAOQFI010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70939_length_1374_cov_3.307051"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="PLE93_10200"
     CDS             complement(<1..1360)
                     /locus_tag="PLE93_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH87463.1"
                     /translation="MPSFMMVMEAVIKAWDEKRDEILAQSGDVRSRLGAVSLIGAPEG
                     DLDPELPKRRAANLAEQADMDLGGFGPAPKFPPASALDFMLGQGETAAAGVTLDRMAA
                     GGIFDQLAGGFARYSVDDRWLVPHFEKMLYDNALLARTYLRAWQTTGRDRYRWVAQKT
                     LDWAMAEMLDPAGGFYASLDADSEGHEGLFYTWTPAEIEEALGQDAGEAIEAFGVTAE
                     GHLEGRSVLHLPGGAPPDEIDPGLEELTDRLLAARSARIRPDLDDKVLSSWNSLMISA
                     LSEAGAVLGRTDYLEAAQRCADFIWNGMRSPQGNLLRTWRQGEARLNAYLEDHAFLLE
                     ALLDLYQACFDPVVFDRARAVAETMIDRFSDPARGGFFTTSHDHEELIARRKDVGDHP
                     IPASSSSAALGLLRLAAMTGESAYRRKAEEVLKLLAPATSRQPAAFGHLLQALAFYHS
                     GGA"
BASE COUNT          218 a          457 c          479 g          220 t
ORIGIN      
        1 cggcgcctcc cgagtggtag aaggccaggg cctggagcag atggccgaac gccgccggct
       61 ggcgggaggt ggccggggcc agcagcttta gtacctcctc ggccttgcgt cgatatgccg
      121 attcaccggt catggccgcg aggcggagca acccgagcgc cgccgaggag gaagaagccg
      181 ggatcgggtg gtctccgacg tccttgcggc gggcgatcag ctcctcatgg tcgtgcgagg
      241 tggtgaagaa gccgccacgc gccgggtccg agaagcgatc gatcatcgtt tccgcgaccg
      301 ccctagcccg atcgaagacg acgggatcga agcaggcctg gtacaggtcg agcagggctt
      361 cgagcaggaa ggcatggtct tcgaggtagg cgttcagtct cgcttcgccc tgacgccagg
      421 tccggagcag gttgccctgc gggctccgca tcccgttcca gatgaagtcg gcacagcgct
      481 gggcggcttc gagatagtcg gtccgtccga gcaccgcgcc ggcctcggac agggccgaga
      541 tcatcagcga gttccaggaa ctgagcacct tgtcgtcgag gtccggccgg atcctcgccg
      601 agcgggccgc gagcagtcgg tcggtcagtt cctcgagacc cggatcgatc tcgtcgggag
      661 gggcgccacc cgggagatgg aggacgctgc gaccttcgag gtggccttcg gccgtgaccc
      721 cgaacgcttc gatcgcttcc cccgcgtctt gaccgagcgc ctcctctatc tcggccgggg
      781 tccaggtgta gaaaagtccc tcgtgaccct cggaatcggc gtcgagcgag gcgtagaagc
      841 cgccggccgg gtcgagcatc tcggccatcg cccagtcgag cgtcttctgg gccacccagc
      901 ggtaccggtc gcgaccggtt gtctgccagg ccctcagata ggtccgggcc agcagggcgt
      961 tgtcgtaaag catcttttcg aagtggggaa ccagccaccg gtcatcgacc gagtagcggg
     1021 cgaagccgcc ggcgagctgg tcgaagatgc cgccggccgc catccggtcg agggtcaccc
     1081 cggcggctgc cgtctcgccc tgacccagca tgaagtcgag cgcggaggcc ggcggaaact
     1141 tcggggccgg cccgaatccg ccgaggtcca tgtccgcctg ctcggccagg tttgcggccc
     1201 ggcgcttcgg cagttcgggg tcgagatctc cttccggggc gccgatcagc gacaccgccc
     1261 cgagcctcga gcgaacatcg ccggactggg cgaggatctc gtcccgcttc tcgtcccagg
     1321 ccttgatcac cgcctccatc accatcatga agctcggcat gccgcgggaa gtgt
//
LOCUS       DAOQFI010000652         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_70958_length_1374_cov_2.602729, whole genome shotgun sequence.
ACCESSION   DAOQFI010000652 DAOQFI010000000
VERSION     DAOQFI010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70958_length_1374_cov_2.602729"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1374)
                     /locus_tag="PLE93_10205"
     CDS             complement(<1..>1374)
                     /locus_tag="PLE93_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87464.1"
                     /translation="AHQIPAGCLGPKPTVTFSADTLSQTAVPLRDGDQLTLAAHVDNK
                     GESACNLSGLSIMVRPPSADEVSSDHFLLIDNVPLGAGYDAEIINHGPYVVDLADDVF
                     ESQLELSWQATIHNGQADETVTGTGPGTKLTVTRPQVELKVIPDVQSGPAPLTVTTVY
                     LLTNTSPVPTAGTPAPGLVPTGSNGPRDVLADAGCGPLNYLSGDLPADTDPVLDPGET
                     WIFTCLRTYPLPGTYASQPVITGLSTADGRAWPEQPLGAEPPSVTALGPDLVVAKSHQ
                     GDLLAGSSGSYELEVTNTGNQPTDGAVTVADQLPAGLTGTAIAGDGWTCTLASLSCSR
                     SDPLGSGNSYPEVTVTVAVSDDPPDNLVNTATVSGGGEPAGAVANNLDEDPTTVRIPS
                     QPTPPSNRIFKVRKVTSRGDGSALIKVAVPGAGVVAADDAGKSDLVQRAWQKSSKART
                     VSLVVKAG"
BASE COUNT          200 a          417 c          499 g          258 t
ORIGIN      
        1 tccggctttc acgaccagcg aaacagtgcg ggctttggag ctcttttgcc atgcccgttg
       61 aaccaggtcg gactttccgg cgtcatcggc agccaccact cctgctccgg ggacggcgac
      121 cttgatcagc gccgaaccgt cgccgcgact ggtcaccttg cgaaccttga agatcctgtt
      181 cgacggaggg gtcggctgcg aggggattcg caccgtggtc ggatcttcat ccagattgtt
      241 ggcgaccgct ccggcgggtt cgccgcctcc gctcacggtc gcggtgttga ccagattgtc
      301 aggcgggtcg tcgctgaccg ccacggtcac ggtcacctcc ggatacgaat tgccggaacc
      361 gagcggatcc gaccggctgc aactcagcga agcgagggtg caggtccatc cgtctccggc
      421 aatggcggtg ccggtcagcc cggcgggcag ctgatccgcg acggtcacgg ccccgtcggt
      481 cggctggttt ccggtgttgg tgacctccag ttcgtaagaa ccggaactcc cggccagcag
      541 atcgccctga tggctcttgg cgaccaccag gtccggcccg agcgcggtca ctgacggtgg
      601 ctccgccccc aggggttgct ccggccaggc ccttccatcg gccgtgctta ggccggtgat
      661 cacgggctgg ctcgcgtagg tgcccggcag cggataggtc ctgagacagg tgaagatcca
      721 ggtttccccc gggtcgagca cggggtcggt gtcggccggc aggtcgccgg aaaggtagtt
      781 gaggggtccg caaccggcgt cggccaggac atctcgtggt ccgttcgatc cggtgggcac
      841 aagtcccggc gccggcgttc cggccgtggg gaccgggctc gtgttggtca ggaggtagac
      901 ggtggtgacg gtgagcgggg ccggtccgga ctggacgtcg gggatcacct tgagctcgac
      961 ctggggccgg gtcacggtga gcttggtgcc gggtccggtg ccggtcacgg tttcgtccgc
     1021 ctggccattg tggatcgtgg cctgccagga gagctcgagc tgcgattcga agacgtcgtc
     1081 ggcgagatcg acgacatagg ggccgtggtt gatgatctcc gcgtcataac ctgcacccag
     1141 gggcacgttg tcgatcagca ggaagtgatc gctcgagacc tcgtcggcgc ttggcggccg
     1201 gaccatgatc gagaggccgc tcaggttgca ggctgactca cctttgttgt cgacgtgggc
     1261 cgcgagggtg agctgatcgc catccctgag tggcactgcc gtctggctga gggtgtcagc
     1321 cgagaaggtg acggttggct tggggccgag acagcctgcg ggaatctggt gggc
//
LOCUS       DAOQFI010000653         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_71528_length_1368_cov_2.853008, whole genome shotgun sequence.
ACCESSION   DAOQFI010000653 DAOQFI010000000
VERSION     DAOQFI010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71528_length_1368_cov_2.853008"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLE93_10210"
     CDS             <1..531
                     /locus_tag="PLE93_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87465.1"
                     /translation="EWLAGTNPRDKDSDDDGVNDLKEKAGVISAYDSETGDLTITLYA
                     GGELTGSVTDETRVHCESEDVADDNGTDEGDDNGTDEGDDDGTDDQGSGESEDRHGGP
                     GSNGEGHDDHGNGEGRGHDGEGPGHEGDCGGDCSVDDLAEGVEVTEAVVKYTASGKVF
                     KELEIVVPAGDTSTQA"
     gene            627..>1368
                     /locus_tag="PLE93_10215"
     CDS             627..>1368
                     /locus_tag="PLE93_10215"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNH87466.1"
                     /translation="MAEAGENVELAPLTTLKLGGPAERLIKVEDTAELIRLVQAADAE
                     REPLFFLAGGSNVVIADQGVPGTTVWIRTAGIDERELDDGRVSLTVAAGENWDALVAR
                     TVDSGLSGIECLSGIPGTVGATPIQNVGAYGQEVSSTIASVLVLDRENAEVTTLAGSE
                     CGFGYRSSRFKGDSRFLVLAVEFILSPSEMSAPVEYAQLAGALEVEVGERVPLPVARE
                     TVLELRRSKSMVVDPADPDSVSAGSFFTN"
BASE COUNT          262 a          434 c          461 g          211 t
ORIGIN      
        1 gagtggctgg ctggaaccaa cccgcgcgac aaggactcgg acgacgacgg tgtcaatgac
       61 ctcaaggaga aggccggtgt catctcggcc tacgattcgg aaaccggcga tctgacgatc
      121 accctttacg cgggcggcga actgaccggt tcggtcaccg atgagacccg cgttcactgc
      181 gaatccgaag acgtcgctga cgacaacgga accgacgaag gtgacgacaa cggaaccgac
      241 gaaggtgacg acgacggaac cgacgatcag ggctccggag aatccgaaga ccgccacggc
      301 ggccccggca gcaatggcga aggccatgac gatcacggca acggcgaagg ccgcggccac
      361 gacggagagg gccccggtca cgaaggtgac tgtggcggtg actgctcggt agacgacctc
      421 gccgaaggcg tggaagtgac cgaagccgtc gtcaagtaca cggcctcggg caaggtcttc
      481 aaggaactgg agatcgttgt cccggcgggg gacacctcca cccaagcgtg atctgacccc
      541 caacagatcg catggaaagg ggccgctgat gcggcccctt tcttgtgtca acacagccgc
      601 tccccgtttt ctggaagcat cggggcgtgg ccgaagccgg agaaaacgtc gaactcgccc
      661 ccttgaccac cctgaagctg ggcgggccgg ccgagcggct gatcaaggtc gaagacacgg
      721 ccgagctgat caggctggtg caggcggctg acgccgagcg ggaaccgctc ttcttcctcg
      781 ccggcgggtc gaatgtggtg atcgccgacc agggggtgcc cgggaccacg gtctggatcc
      841 ggaccgcggg aatcgacgag cgtgaactcg atgacggccg ggtctctctc accgtcgctg
      901 ccggcgagaa ctgggatgcc ctggtagccc ggacggtcga ttcggggctc tcgggaatcg
      961 agtgcctctc gggaatcccc ggaacggtcg gcgccacccc gatccagaac gtcggggcct
     1021 acggtcagga agtctcatcg accatcgctt ccgtcctggt gctcgatcgc gagaacgccg
     1081 aggtcaccac cctcgccgga agcgaatgcg gcttcggtta ccgttcgagc cgcttcaagg
     1141 gtgattcgcg ctttctcgtg ctggcggtcg agttcatcct cagcccttcc gagatgtcgg
     1201 cgccggtcga gtacgcccag ctcgccgggg cgctcgaagt ggaggtcggc gaacgggttc
     1261 ccctgccggt cgcccgcgaa accgtgctcg agctgaggcg ctcgaagagc atggtggtcg
     1321 acccggccga tccggattcg gtcagcgccg gctcgttttt caccaacc
//
LOCUS       DAOQFI010000654         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_72075_length_1363_cov_1.922783, whole genome shotgun sequence.
ACCESSION   DAOQFI010000654 DAOQFI010000000
VERSION     DAOQFI010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72075_length_1363_cov_1.922783"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLE93_10220"
     CDS             complement(<1..420)
                     /locus_tag="PLE93_10220"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH87467.1"
                     /translation="MSSSLVTGGQGFIGGHLVRALLERGDEVTVLDLRGAPGHAIELH
                     GLTGEVRLLEGDVADPAAVHRAIRESAAGSSGPDSVFHLAAQTLVGPAAEDPVATFRA
                     NAEGTWVLLEACREAEVGAVVVASSDKAYGPSEALPYR"
     gene            complement(417..1157)
                     /locus_tag="PLE93_10225"
     CDS             complement(417..1157)
                     /locus_tag="PLE93_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNH87468.1"
                     /translation="MPAAAENLKPPVLILSGGRGTRLREQTDSLPKPLVEVGGKPLIW
                     HVIQIYARQGFTRFFPLTGYLGEQIEEFVEGEVWPEGVSVSCLETGLDTPTGGRIAAA
                     SGQVEGTFCATYADGVADLDLDALLDFHRSSGRVATMTAVRPDLQWGVAEIDPAGEVT
                     GFIEKPRSEHWINGGFFCFEPAVFDWLESDSVLEREPLENLAAAGQLGAFRHEGFWDC
                     CDTYKDLVTLNDLWAGGEARWLGSKATA"
     gene            complement(1141..1356)
                     /locus_tag="PLE93_10230"
     CDS             complement(1141..1356)
                     /locus_tag="PLE93_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNH87469.1"
                     /translation="MSAEGATVGEVLDAVFAEHGDLRDRITEGGELRRFVNVYVGGED
                     IRFGDGLGTAVSDGAEVTILPAVAGGC"
BASE COUNT          235 a          480 c          446 g          202 t
ORIGIN      
        1 gcggtagggc aacgcctcgc ttggcccgta ggccttgtcg gaggaggcga cgacgaccgc
       61 gccgacctcc gcctcgcggc aggcctcgag cagcacccag gttccctcgg cgttggcgcg
      121 gaaggttgca accggatcct cggccgccgg cccgaccagg gtctgggcgg ccaggtgaaa
      181 gaccgaatcc ggccccgacg aaccggccgc tgactcgcgg atcgcgcgat gaacggcagc
      241 cgggtcggcg acgtcgcctt cgaggagtcg cacctcgccg gtcagtccgt gcaactcgat
      301 cgcatgaccg ggagcgccac gcaggtccag caccgtgacc tcatcgcccc gctccagcag
      361 cgcccggacg aggtgccccc cgatgaaacc ctggccgccg gtgaccagcg aggaactcaa
      421 gcggttgcct tcgagccgag ccagcgtgcc tcgccaccag cccagagatc gttcagggtg
      481 accaggtcct tgtaggtgtc acagcagtcc cagaaccctt cgtgacggaa ggcaccgagc
      541 tgccctgccg cggccaggtt ctcaagcggc tcgcgctcga ggaccgagtc cgactcgagc
      601 cagtcgaaga cggcaggttc gaaacagaaa aagccgccgt tgatccagtg ctcggacctc
      661 ggcttctcga tgaagccggt gacctcgccg gccggatcga tttccgccac gccccactgc
      721 aggtccggcc ggaccgcggt catggttgcc accctgccgc tggagcggtg aaagtcgaga
      781 agtgcgtcga ggtcgaggtc ggcgacaccg tcggcgtagg tcgcacagaa ggttccctcg
      841 acctgaccgc ttgccgcggc gattcgaccg ccggtcgggg tgtcgagccc ggtttccagg
      901 caactcacgg aaacgccctc cggccagact tcgccttcga cgaactcctc gatctgctcg
      961 ccgaggtaac cggtcagcgg gaagaacctg gtgaatccct gccgggcgta aatctggatc
     1021 acatgccaga tcagcggctt gccaccgacc tcgaccagcg gcttgggcag ggaatcggtc
     1081 tgctcgcgca gccgcgtgcc ccggccgccg ctgaggatca gaaccggggg cttcaggttt
     1141 tcagcagccg ccggcaacgg ccggaaggat cgtcacttcg gcaccatcgg aaaccgcggt
     1201 gccgagcccg tcgccgaagc ggatgtcttc accgccgacg tagacgttta cgaagcgacg
     1261 cagctcgccg ccttcggtga tccggtccct gaggtcgccg tgttcggcga agacagcatc
     1321 gaggacctcg cccaccgtgg caccctcggc gctcaggtcg gcc
//
LOCUS       DAOQFI010000655         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_72484_length_1359_cov_2.562883, whole genome shotgun sequence.
ACCESSION   DAOQFI010000655 DAOQFI010000000
VERSION     DAOQFI010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72484_length_1359_cov_2.562883"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLE93_10235"
     CDS             <1..211
                     /locus_tag="PLE93_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNH87470.1"
                     /translation="IEPDMFTVEGEIESKVYLGVGNQLTLKLAGGGRLVALEQATYRM
                     RAEEQWEVGQRVRIGWHPEHCMVLS"
     gene            complement(218..991)
                     /locus_tag="PLE93_10240"
     CDS             complement(218..991)
                     /locus_tag="PLE93_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH87471.1"
                     /translation="MSLVRTERDGPMAVITLNSPPLNLFDRAMTEELMAAFEAIQNDP
                     PRGLLVRAEGEVVSGGVNVNEFKGLTAAEGTRLWEQLLGLIDRVEGFPFPVVFAAHGL
                     TLTAAFELSLACDLIIASESAHFGLVEIVVGLTPSMGGPQRLVERAGSGRARQLVMTG
                     GLFDAATLERWNVVNEVKPDEEIDAYARKLTKRLANGPTLAHAMTKRIVRAWRDEGMA
                     GADRVTRRDAAELFESEDLKNAVDSFLENGPGKATYEGR"
     gene            complement(995..>1359)
                     /locus_tag="PLE93_10245"
     CDS             complement(995..>1359)
                     /locus_tag="PLE93_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87472.1"
                     /translation="TPRAKNLQAGGIALFANAFERLGVTGEAAGDAFHHYGSYCFGTA
                     LYAASRLNARAALGPDRSDHGELETLREEAGVTGTSDEKTRAALDRVLDLSEIDPARD
                     EHLFVLGLKQILKGFARV"
BASE COUNT          211 a          436 c          477 g          235 t
ORIGIN      
        1 gatcgagccg gacatgttca cggtcgaggg cgagatcgaa agcaaggtct acctcggggt
       61 cggcaaccag ctcaccctga agctggcggg cggcggccgg ctggtcgccc tcgagcaggc
      121 gacctaccgg atgcgggcgg aggagcagtg ggaggtcggc cagcgcgtca ggatcggctg
      181 gcaccccgaa cactgcatgg tcctctccta gggttcgtca gcggccttcg taggtcgcct
      241 tgccggggcc gttctcgagg aaggagtcga ccgcgttctt gaggtcctcc gactcgaaga
      301 gttcggccgc gtcgcgcctg gtgacccggt cggcgccggc cattccttca tcccgccagg
      361 cccggacgat ccgcttggtc atcgcgtggg cgagggtcgg cccgttggcc aggcgtttgg
      421 tcagcttgcg ggcgtaggca tcgatttcct cgtccggctt gacttcgttg accacgttcc
      481 agcgttcgag ggtcgccgcg tcgaagaggc ctccggtcat caccagttgc cgggcgcggc
      541 ccgagccggc cctttcgacc agccgctgcg ggccacccat cgagggggtc aggccgacca
      601 cgatctcgac caggccaaag tgggccgatt cagaggcgat gatcaggtcg caggccagcg
      661 agagctcgaa ggccgcggtc agggtcagac cgtgagcggc gaagacgacc gggaagggga
      721 agccctcgac ccggtcgatc agccccaaaa gctgttccca gagccgggtg ccctcggcgg
      781 cggtcaggcc cttgaactcg ttcacgttga ccccgccaga gacgacttca ccctcggccc
      841 ggaccaggag accgcggggc gggtcgttct ggatcgcctc gaaggcggcc atgagttctt
      901 cggtcatcgc ccggtcgaag aggttcagcg gcggactgtt cagcgtgatc acggccatcg
      961 gcccgtcgcg ctctgttctg actagtgaca tgcctcagac tctggcgaat cccttgagaa
     1021 tctgcttcaa gcccagtacg aagagatgct cgtcccgggc gggatcgatc tcggaaaggt
     1081 cgagcacccg gtcgagcgcg gcccgggtct tctcgtccga agtgccggtt accccggctt
     1141 cctcgcgcag ggtttccaat tcgccgtggt cggatctgtc gggtcccagg gcggcccggg
     1201 cgttgagtcg gctggccgcg taaagcgcgg tgccgaagca gtagctcccg taatggtgga
     1261 aggcgtctcc ggccgcctcg ccggtaaccc cgagcctctc gaaggcgttg gcgaagaggg
     1321 cgatgccgcc ggcctgaagg ttctttgcgc ggggagtga
//
LOCUS       DAOQFI010000656         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_72664_length_1357_cov_4.459293, whole genome shotgun sequence.
ACCESSION   DAOQFI010000656 DAOQFI010000000
VERSION     DAOQFI010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72664_length_1357_cov_4.459293"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLE93_10250"
     CDS             complement(<1..819)
                     /locus_tag="PLE93_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH87473.1"
                     /translation="MAEQTATAAASPEEAMVSSPDSPELREVTGPTALGGGWRRSLDL
                     LYLISINDFKKTYFDTVLGYVWSLFRPLILFAVLLFVFTQIFRIGDDVPHYPVMLLFN
                     IVVFTFFQEATIAAVTSVVAQEGVVRKTQFPRVVIPLAVVLTALFNFGVNLVVVLVFL
                     LASGVPVEWTWLFFPLLVLVLTVITTAVCLLVSSLFVRFRDVGIIWGVLATALFYATP
                     VLYPIEVVPERFHDIVLINPLTPIFIELRHLVIDNSAPTAVSAAGGWLGLLPAVA"
     gene            complement(812..>1357)
                     /locus_tag="PLE93_10255"
     CDS             complement(812..>1357)
                     /locus_tag="PLE93_10255"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HNH87474.1"
                     /translation="ARAYLKLNFDAETPRQETLGFLEDGEVEDDFIPGVDIQAKLIDA
                     WLDDGAGERVTNVEIGDPLRLNLEIEALRDLERPLFTFQINDAKHTQVTGFNQTLEVE
                     GPGDVIHEGQKVTISGEIVNRLTPGRYLLTCWVTRNRETSERVVQGIKLFEFVIFGMK
                     RTPGLVEAEASLNAKVAGSNG"
BASE COUNT          262 a          423 c          459 g          213 t
ORIGIN      
        1 ggcgaccgcg gggagcagac cgagccagcc gccggcggcg ctgaccgcgg tcggggcgga
       61 gttgtcgatc accaggtgcc gcagttcgat gaagatcggg gtgagcgggt tgatcagcac
      121 gatgtcgtgg aagcgctccg gcaccacctc gatcgggtag aggaccgggg tcgcataaaa
      181 gagcgccgtg gccagcacgc cccagatgat cccgacgtcc cggaatctga cgaaaagcga
      241 ggagaccagc aggcagaccg cggtcgtgat caccgtgagg acgagaacca ggagcgggaa
      301 gaacagccag gtccactcga ccggaacccc ggaggcgagc aggaagacca ggaccacgac
      361 caggttcacg ccgaagttga acagcgcggt cagcaccacc gcgagcggga tcaccacccg
      421 cgggaactgg gtctttcgca ccaccccttc ctgggccacg actgaggtca ccgcggcgat
      481 cgtcgcctcc tggaagaagg tgaagacgac gatgttgaag agcagcatca ccgggtaatg
      541 cggcacgtca tcgccgatcc ggaagatctg ggtgaagacg aagagcagca ccgcgaagag
      601 gatcagcggc cgaaacagcg accagacgta tcccagcacc gtgtcgaagt aggtcttctt
      661 gaagtcgttg atcgagatca ggtagagcaa atccagcgag cggcgccagc cgccaccgag
      721 cgcggtgggg ccggtcactt cccgcagttc gggtgaatcc ggactggaga ccatcgcctc
      781 ttcgggggat gccgccgcag tcgccgtttg ctcagccatt gctgcccgcc accttcgcgt
      841 tgaggctggc ttccgcctcg accagtccgg gggtgcgctt catcccgaag atcacgaact
      901 cgaagagctt gatcccctgg accacgcgct ccgaagtctc ccggttgcgg gtgacccagc
      961 aggtcagcag gtagcggccg ggggtgagcc ggttgacgat ctcgccgctg atcgtcacct
     1021 tctggccctc gtggatcacg tcgccggggc cctccacctc gagggtctgg ttgaagccgg
     1081 tgacctgggt gtgcttggcg tcgttgatct ggaaggtgaa aagcggccgt tcgaggtcac
     1141 ggagggcctc gatctccagg ttgagccgca gcggatcacc gatctcgacg ttggtcaccc
     1201 gctcgccggc gccgtcgtcg agccaggcgt cgatcagctt cgcctggatg tcgaccccgg
     1261 ggatgaagtc atcctcgacc tcgccatcct caaggaagcc gagggtttcc tgccgcggcg
     1321 tttcggcgtc gaagttcagc ttcaggtagg cccgggc
//
LOCUS       DAOQFI010000657         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_72698_length_1357_cov_2.751920, whole genome shotgun sequence.
ACCESSION   DAOQFI010000657 DAOQFI010000000
VERSION     DAOQFI010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72698_length_1357_cov_2.751920"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLE93_10260"
     CDS             <1..336
                     /locus_tag="PLE93_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNH87475.1"
                     /translation="VEVDGERMLFVESLETVKCLDEGVIESVADANIGSIFGIGFPGW
                     TGGVLQYIDGYTSEAHPERRGPAGFVERSRELAEKYGERFEPPASLVEKAERGETYSG
                     QPVRDLVAS"
     gene            379..1056
                     /locus_tag="PLE93_10265"
     CDS             379..1056
                     /locus_tag="PLE93_10265"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNH87476.1"
                     /translation="MPARPLSQVIDPGWAEALEPVSGQIASMGDFLRGESAAGRGYLP
                     AGENILRAFTLPLDQVRVLIVGQDPYPTPGHAVGLCFSVAPDVRPLPPSLVNIFKEYS
                     DDLGYPPPENGDLSPWFDRGVLLLNRSLSVQPGRPNAHQGKGWEGVTEQAITALARRG
                     GPLVAILWGRNARNLAPLLGPVPRIESAHPSPMAAHNGFFGSKPFSRANQLLADQGAE
                     PIDWRLP"
     gene            1168..>1357
                     /locus_tag="PLE93_10270"
     CDS             1168..>1357
                     /locus_tag="PLE93_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNH87477.1"
                     /translation="MRLIVARCEARYTGRLDTFLPEALRLVMIKSDWSVMIHADTGGY
                     KPQNWMTPPTTIEEIQAEG"
BASE COUNT          202 a          456 c          471 g          228 t
ORIGIN      
        1 gttgaagtag atggtgagcg gatgcttttc gttgaatcgc tggaaacggt caagtgcctc
       61 gacgagggcg tgatcgaatc ggtggccgac gccaacatcg gctcgatctt cgggatcggc
      121 ttcccgggct ggaccggcgg cgtgctccag tacatcgacg gctacacctc ggaagcccac
      181 cccgagcgcc gcggcccggc gggcttcgtc gagcgcagcc gggagctggc cgaaaagtac
      241 ggcgagcgtt tcgagccgcc ggcttccctg gtcgaaaagg ccgagcgtgg cgagacctac
      301 tccggccagc cggtccggga tctggtcgct tcctgacctt ccgcgggcgg cgccggcgcg
      361 aactcgtatt ctgggttcgt gccggcccgt ccgctaagtc aggtaatcga ccccggctgg
      421 gccgaggcgc tcgaaccggt ttccgggcag atcgcctcga tgggggactt cctccggggc
      481 gaatcggccg cgggccgcgg ttacctgccg gccggggaga acatcctcag ggccttcacc
      541 ctgccgctcg atcaggtccg ggtgctgatt gtcgggcagg acccgtaccc gaccccgggg
      601 catgccgtcg ggctctgctt ctcggtcgcc ccggatgtgc ggccgctgcc accgagtctg
      661 gtcaacatct tcaaggagta cagcgacgac cttggctatc cgccgccgga aaacggcgac
      721 ctttctccct ggttcgaccg cggggtgctg ctgctgaacc ggtcgctatc ggtccagccc
      781 ggccggccga acgcgcatca gggcaagggc tgggaggggg tgaccgaaca ggcgatcacc
      841 gcgctggccc ggcggggcgg gccgttggtc gcgatcctct ggggccggaa cgcccgcaac
      901 ctggcgcctt tgctcggccc ggtgccgcgg atcgaatcgg cccatcccag cccgatggcc
      961 gcccacaacg gcttcttcgg ctcgaagccc ttcagccggg ccaaccagct cctggccgac
     1021 cagggggccg agccaatcga ctggcgcctc ccgtagaggc gcggtgggtc accgaatgcc
     1081 cactttgtgc cggtatgcgg cgcaaagtgg gcactcgcgg cgcgcgggcg gccagcggcg
     1141 acctgaggct tggctactgt tccgatcgtg cgtttgatcg ttgctcgttg cgaggcccgc
     1201 tataccggcc gtctggacac ctttctgccg gaggcgctcc ggctggtgat gatcaagtcg
     1261 gactggtcgg tgatgatcca cgccgacacc ggtggctaca agccccagaa ctggatgact
     1321 cccccgacca cgatcgagga gatccaggcg gaaggcg
//
LOCUS       DAOQFI010000658         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_73006_length_1354_cov_3.136259, whole genome shotgun sequence.
ACCESSION   DAOQFI010000658 DAOQFI010000000
VERSION     DAOQFI010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73006_length_1354_cov_3.136259"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..927
                     /locus_tag="PLE93_10275"
     CDS             112..927
                     /locus_tag="PLE93_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNH87478.1"
                     /translation="MKIFSWNVNGIRAVIRKGDFGSFVEKYDPDILCMQETKAEQGQA
                     EIDLPQYEEYWNSAGKKGYSGTAIFTRQKPIAVLNDFAAEISDEYDLHNDSYGDPRAE
                     GRVITAEYDHFFLVTTYVPNAKRDLSRLKLREKQWDPALLHHMKMLDAKKPVLVCGDM
                     NVAHTEDDLANPKANAGEHGFTDEERQGFQNFLDAGFLDTFRLFAEGNGHYSWWSYMS
                     KARERNAGWRIDYFLASERLRQAITGAEILGDVMGSDHCPVSVELDEKKLAKL"
     gene            complement(994..>1354)
                     /locus_tag="PLE93_10280"
     CDS             complement(994..>1354)
                     /locus_tag="PLE93_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87479.1"
                     /translation="TDVGPTTRVASPETMQSCVGLAGHAKLKAKVVKKPRAVRNGGRG
                     FFRVYVRNYGTATAKGVYLFAPSVKKKVGWIAPGVARTIGIRARVRGRKGHSRTIRFT
                     VKGNGVSASAFARVRVK"
BASE COUNT          262 a          452 c          413 g          227 t
ORIGIN      
        1 gtcgccggaa ccagaatcga aatcgctatc gcgatcaccg ttgccaggcc ctgccagcct
       61 ctcccctccc acatgccccg aatcctaccc gccgggtagt ctggcgggcc gatgaagatc
      121 ttctcctgga acgtcaatgg cattcgggcc gtcatccgca agggtgactt cggctcattc
      181 gtcgagaagt acgaccccga catcctctgc atgcaggaga ccaaggcgga gcagggccag
      241 gccgagatcg acctcccgca atacgaggag tactggaact cggccgggaa gaagggctac
      301 tccggaaccg cgatcttcac ccggcagaag ccgatcgcgg tcctgaacga tttcgccgcc
      361 gagatctcgg acgagtacga cctgcacaac gattcatacg gcgacccgcg ggccgagggc
      421 cgggtgatca ccgccgaata cgaccacttc ttcctggtta cgacctatgt gccgaacgcc
      481 aagcgggacc tctcccggct gaagctgcgc gagaagcagt gggatccggc cctgctccat
      541 cacatgaaga tgctggacgc gaaaaagccg gtcctggtct gcggcgacat gaacgtcgct
      601 cacaccgagg atgacctggc caacccgaag gccaatgccg gcgagcacgg cttcacggac
      661 gaggagcggc aggggttcca gaactttctc gacgccggct tcctcgacac cttccgcctc
      721 ttcgccgaag gcaacggcca ttactcctgg tggtcgtaca tgtcgaaggc ccgggagcgg
      781 aacgccggct ggcggatcga ttacttcctt gcctccgaac gcctcaggca ggcaatcacc
      841 ggggcggaga tcctgggtga tgtcatgggt tccgaccact gcccggtcag cgtcgagctc
      901 gatgaaaaga aactggcgaa gctgtagggc agcccgaccg tgaagaaggg cggccgcctt
      961 ggccgccgcc cttcccgata cgcgtacgcg gcgctatttc acccggaccc tcgcaaaggc
     1021 ggaggcggac acgccatttc ccttgaccgt gaacctgatg gtccgggagt gacctttccg
     1081 gccgcgaacc ctggcccgga tgccgatcgt cctggcgacg ccgggagcga tccagccgac
     1141 cttcttcttg accgagggcg caaacaggta aacgcccttt gccgtggccg ttccgtagtt
     1201 ccttacatag acgcggaaga accccctgcc gccgttccgg accgcgcgcg gcttcttcac
     1261 aactttcgcc ttgagcttgg cgtgaccggc cagaccgacg cagctctgca tggtctcggg
     1321 ggaagcgacc cgggtggtcg gaccgacgtc ggtg
//
LOCUS       DAOQFI010000659         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_73107_length_1353_cov_3.698767, whole genome shotgun sequence.
ACCESSION   DAOQFI010000659 DAOQFI010000000
VERSION     DAOQFI010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73107_length_1353_cov_3.698767"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLE93_10285"
     CDS             complement(<1..415)
                     /locus_tag="PLE93_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH87480.1"
                     /translation="MSSSSANPPDPSPPALEITDLVKRYPTGVEALKGVSLEIADGEF
                     FGLLGPNGAGKSTLIHCSTGLASPTSGEIRVFGHDAVGDYRQAREAVGLAPQELNIDW
                     FLTVEETLDFHGGYFGMGKKDRKERVDELLESFSLV"
     gene            447..1322
                     /gene="thrB"
                     /locus_tag="PLE93_10290"
     CDS             447..1322
                     /gene="thrB"
                     /locus_tag="PLE93_10290"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNH87481.1"
                     /translation="MTERHRLVRVPASSANLGPGYDVMAVALDLHLELEVKETGTFSV
                     SCDALDVPLDRSNLVVRAFETLQPADGISFRVGGEIPLARGMGSSAAAIVAGLVAADH
                     LFELGLERQDLLERATALEGHPDNVAAAICGGFVICTGGPRLTATVIEAPEGLEGILV
                     IPEVEVSTRKAREAIPEQIPIKDAVANTASAAHLALGLARGDSDLIGMGLHDRLHQER
                     RRHLFPKSMEIVEVAGEIGAIGATISGAGPTVLVWTNWQETGGVVEQLRERCEGWAEV
                     RRVGFSPLGADVPEL"
BASE COUNT          228 a          447 c          455 g          223 t
ORIGIN      
        1 cgaccaggga aaaggattcg agcagctcgt cgactcgttc cttgcggtcc ttcttgccca
       61 tgccgaagta gccgccgtgg aagtcgaggg tctcctcgac cgtgaggaac cagtcgatgt
      121 tcagttcctg cggcgccagc ccgaccgcct cgcgggcctg ccggtagtcg ccgaccgcgt
      181 cgtggccgaa gacccggatc tcgcccgagg tcgggctggc gagaccggtc gagcaatgga
      241 tcaaagtcga cttgcccgcc ccgtttggac cgagcaggcc gaagaactcg ccgtcggcga
      301 tctcgagcga aacgcccttc agggcttcga caccggtcgg gtaacgcttg accagatcgg
      361 tgatttcaag cgcagggggg gacggatcag gtggattggc ggaggacgaa ctcacccctc
      421 aatcattagc aaggtatttt tgccgggtga ctgaacggca tcgactcgtc cgggttcccg
      481 cttcgtcggc caatctcgga ccgggctacg acgtgatggc ggtggctttg gatcttcacc
      541 tcgaactcga ggtgaaggag acggggacct tctcggtcag ctgcgacgcg ctcgacgtgc
      601 cgctcgatcg cagcaacctt gtggtccgcg ccttcgaaac cctgcagccc gcggacggca
      661 tttccttccg ggtcggcggc gagatccccc tcgcccgcgg catgggatcg agcgccgccg
      721 cgatcgtcgc cggactggtc gcggccgacc acctgtttga actcgggctc gaacggcagg
      781 atctgcttga acgcgccacg gctctcgagg gccatcccga caacgtcgcc gccgcgatct
      841 gcgggggctt cgtgatctgc accggcggcc cgcgactgac cgcaaccgtg atcgaagcgc
      901 cggaaggcct ggaggggatc ctggtcatcc cggaggtcga ggtttccacc cgcaaggccc
      961 gcgaggcgat cccggaacag atcccgatca aggacgcggt cgccaacacg gcctccgccg
     1021 cccaccttgc cctgggcctg gccaggggtg actccgacct gatcggaatg gggctccacg
     1081 accggctcca ccaggaacgg cgccgccacc tcttcccgaa atcgatggag atcgtcgaag
     1141 tggccggcga gatcggggcg atcggcgcga cgatctccgg ggccggaccc accgtcctgg
     1201 tctggaccaa ctggcaggaa accgggggag tggtcgagca gttgagggag cgttgcgaag
     1261 gctgggccga ggtaaggcga gtcggcttca gccccctcgg cgccgacgtc cccgagctct
     1321 gagatggacc tgctcgtccg gatcggcctg atc
//
LOCUS       DAOQFI010000660         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_73361_length_1351_cov_2.896605, whole genome shotgun sequence.
ACCESSION   DAOQFI010000660 DAOQFI010000000
VERSION     DAOQFI010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73361_length_1351_cov_2.896605"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(437..832)
                     /locus_tag="PLE93_10295"
     CDS             complement(437..832)
                     /locus_tag="PLE93_10295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNH87482.1"
                     /translation="MPRDSHPSATVHRRIEFSDVDSSGRYHYGTVIRLLEAAEVELLS
                     GLGLLDSIYTSMPRARLEVDYLSTLRFMDEVDVTVEVEDLGRTSVTIGFTVERGGEAC
                     ARGRLVTVFVDQQGTPAPWPAEARTAFGG"
     gene            complement(853..926)
                     /locus_tag="PLE93_10300"
     tRNA            complement(853..926)
                     /locus_tag="PLE93_10300"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(890..892),aa:Pro,seq:tgg)
BASE COUNT          252 a          440 c          451 g          208 t
ORIGIN      
        1 tggcccagcc gcctcgacct ccctgagcag atgatccggg tccgaaacct ctcccctggg
       61 ctccgtacag attgaaggtc gccgaggtga cgccgtaagg aaccgtccag gactccgggg
      121 caccggtgta gttgaaggtc acggtcgtcg cccctgccga cgacggcagc acgaaggcgc
      181 acaccaacgc aagtcccccc agcaacgatc ccgttagacg tctcaacttc ccccgattcc
      241 cgtttttgcc cgaaggcgga ttcatctcga tcctatcggg catcgcccgg gaaatcgaac
      301 aacgatcaga aacatcgaga aaccggggcg gccgggtcaa acgctcgcaa gcgggccaca
      361 agttcttgcc acgcccgcct cgtcgaatgt ccggtcgaag tgatcgatcc cctgggtgct
      421 tgcgcccccg cggctttcag ccgccgaagg cggtgcgggc ttcggcgggc cagggggccg
      481 gtgtgccctg ctggtcgacg aaaacggtga ccaggcggcc tcgggcgcag gcctctcccc
      541 cgcgttcgac cgtgaatccg atggtgaccg aggtgcggcc gagatcctcg acctcgaccg
      601 tcacgtctac ctcgtccatg aaccgcaggg tcgagagata gtcgacctcc agccgggccc
      661 gcggcatcga ggtgtagatc gaatcgagca aacccagccc ggaaagcagc tcgacctcgg
      721 ccgcctccag caaccggatc acggtcccgt agtggtaccg gcccgaagaa tcaacatcgg
      781 aaaactcgat ccggcgatga acggtggctg aggggtggga atctcgaggc atgggcgcgg
      841 gtgttcgacc aatcggggtg cccggattcg aaccggggat ctttcgcccc caaagcgaac
      901 gcgataccag gcttcgctac accccgaagc ggtgacgatt ctacttccac tcgcctcgga
      961 tgcgatccat ccatgcccga agcctgacgc gcagtcggtg tcgcttgacc ccacctgaag
     1021 gaccccgttg ctcgtggaat cgagaaggga gaagttggta catagcgctc ccaactccga
     1081 tctcgatcgg ccgctcccga tcgagctcgg agttgggtgc gatatgtggc gcaaagtgga
     1141 acctggcggt ctgggtccgg ggccggccag agaccccggg actcaacggg cggggccggg
     1201 gcccgggccg gacttggaga gaccccggga atggcggcta gcggggcccg ggccggactc
     1261 ggagagaccc agggaatggc ggctggcggg gccgggccgg cggcgtggga ggttcagagt
     1321 ccctgggact ggcgccaggc gtggaaggcc t
//
LOCUS       DAOQFI010000661         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_73657_length_1348_cov_4.300077, whole genome shotgun sequence.
ACCESSION   DAOQFI010000661 DAOQFI010000000
VERSION     DAOQFI010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73657_length_1348_cov_4.300077"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLE93_10305"
     CDS             complement(<1..757)
                     /locus_tag="PLE93_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87483.1"
                     /translation="MVEPEFDSEIELGLEVKIPGQLPAGRADALFLYGYAFHRRQDVE
                     GLEILVDGQPNRAVGRMPRLDLFKSLHPLIPEGEVSETHDAGSLEDPELKSFRSGFWS
                     TVEIPATGPGKVRIEVALKLADGSISRAPLAEIEMVDQEQRFSTDIEEDRGSRPPEAR
                     IAICMATHNPDIGLLRIQLDSLREQTVTDWVCLISDDCSLPETFREIEAEIEGDDRFL
                     ISRSEARLGFYRNFERALGMVPETAELVALCDQD"
     gene            complement(817..>1348)
                     /locus_tag="PLE93_10310"
     CDS             complement(817..>1348)
                     /locus_tag="PLE93_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87484.1"
                     /translation="GGVLAADSPKMFFEMVDSLEQAGVRDVVEGYLGERAAISAQKCT
                     LRKADPSVKGGWHQDGKFLGDVRALNVWLSLSRCGDVAPSMDIVPVRIDEFVAAGVNE
                     GDLHYQVTDETAAEAAGETGIVRPIFNPGDVLLFDEMFLHQTGSDPSMPNPRYAIESW
                     FFGPSAFPEEYAPIAF"
BASE COUNT          237 a          439 c          445 g          227 t
ORIGIN      
        1 catcctgatc acagagcgca accagttcgg ctgtctcggg gaccatgccg agcgcccgct
       61 cgaagttgcg gtagaagccc agcctcgcct cggaccggga gatcaggaag cggtcgtcgc
      121 cttcgatctc cgcctcgatc tcgcggaagg tctccgggag cgagcagtcg tcgctgatca
      181 ggcagaccca gtcggtcacc gtctgctcac gcagcgaatc aagctggatc ctgagcagcc
      241 cgatgtccgg gttgtgggtc gccatgcaga tcgcgatccg tgcctccggg gggcgggacc
      301 cacggtcttc ctcaatatct gtggaaaacc gttgttcctg atcgaccatc tcgatctcgg
      361 cgaggggggc gcggctgatg ctgccatccg caagcttcag ggccacttcg atcctgacct
      421 tgcccggtcc ggtggccggg atctcgaccg tggaccagaa accggagcgg aagctcttca
      481 gttcagggtc ctccagcgag ccggcgtcat gggtctcgga gacttcgccc tccgggatca
      541 gcgggtgaag cgacttgaag aggtcaaggc ggggcatgcg cccgaccgcc cggttcggct
      601 ggccgtcgac caggatctcc aggccctcga cgtcctggcg gcggtgaaat gcgtacccgt
      661 aaaggaagag cgcgtcggcc cggccggccg gcagctgccc ggggatcttt acctccagcc
      721 cgagctcgat ctcggaatcg aattcaggct cgaccattca gacgagacta ttgggaactc
      781 gggccggatg tggccgggaa cggcctcccg cccggctcaa aatgcgatcg gcgcgtactc
      841 ctcggggaag gccgagggtc cgaagaacca gctctcgatc gcgtaacgcg ggttcggcat
      901 cgaaggatcc gagccggtct ggtggaggaa catctcgtcg aagaggagca cgtcaccggg
      961 gttgaagatc ggccggacga tgccggtttc acccgccgct tcggccgcgg tctcgtcggt
     1021 cacctggtag tggaggtcac cttcgttgac gccggctgcg acgaactcgt cgatccggac
     1081 cgggacgatg tccatgctcg gggcgacatc accgcagcgc gaaagcgaaa gccacacgtt
     1141 cagggcacgg acgtcaccga ggaacttgcc gtcctgatgc cagccgccct tcacgctcgg
     1201 atcggccttg cgcagggtgc atttctgggc ggaaatggcg gcccgctcgc cgaggtaacc
     1261 ctcgaccacg tcgcgaaccc cggcctgctc gagcgagtcg accatctcga agaacatctt
     1321 cggcgagtcg gccgcgagga ccccgccg
//
LOCUS       DAOQFI010000662         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_74665_length_1339_cov_3.687695, whole genome shotgun sequence.
ACCESSION   DAOQFI010000662 DAOQFI010000000
VERSION     DAOQFI010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74665_length_1339_cov_3.687695"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLE93_10315"
     CDS             <1..196
                     /locus_tag="PLE93_10315"
                     /inference="COORDINATES: protein motif:HMM:NF018531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNH87485.1"
                     /translation="EDEAIRVLETARRQMQDAVETGKRPGRIYKKTGRPCPRCRAARI
                     RSRGQGEDNRTTYWCPNCQA"
     gene            336..1025
                     /locus_tag="PLE93_10320"
     CDS             336..1025
                     /locus_tag="PLE93_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH87486.1"
                     /translation="MTSAEESTVGLLRSVPLFAELDQDELEQFSRVAIPRAYPEGTRV
                     FHEGDHSDACYIVKSGTFRVTREHPDGRAITLATLTEGDIVGELAMLDGEVRSASVET
                     LGGDGELLALPASDVRALLERHPDITVKFVAALTRRLRAANERISRQSFQTVPSRVAG
                     VLSQLVAEEGEDGRQVTIRMNQADLAQLAGTSRESVSRFLADLERSGVVKAGRGQVTI
                     LEPARLGNYIF"
     gene            1035..>1339
                     /locus_tag="PLE93_10325"
     CDS             1035..>1339
                     /locus_tag="PLE93_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNH87487.1"
                     /translation="MSGLLALNFYSPVFVDQLKRGRKTATIRLGNKSAKYQRSQIVWV
                     TVGSRHSRREKIFSAVIDEVEVKRLSELSPRDIERDNPEFRRVEETRDFLQKIYDRE"
BASE COUNT          255 a          438 c          426 g          220 t
ORIGIN      
        1 tgaggacgag gcgatccgcg tgctcgaaac cgcccgccgg cagatgcagg acgcagtcga
       61 gaccggcaag cggccgggcc gcatctacaa aaagaccggc cgcccctgcc cccgctgccg
      121 cgcggccaga atccgctccc gcggccaggg agaagacaac cgcaccacct actggtgccc
      181 gaactgccag gcctgatccg actcccgtcc aaaccggggg cgatcggcca ccgttttggt
      241 gggtaaacgc ccccggaagg cacctgggaa gggtttctga cctcaattga tcagaatcgt
      301 tttgtttttc cggtcggatg cgaaatactc cgcgggtgac ttcggctgag gagagcacag
      361 tcgggctgct gagaagcgtg ccccttttcg ccgagcttga tcaggacgag ctcgagcagt
      421 tttcgcgtgt cgcgattccg cgtgcatacc ccgaagggac cagggtcttc cacgagggag
      481 accattccga cgcctgttac atcgtcaagt ccggcacctt ccgggtaacc cgcgagcatc
      541 cggacggcag ggccataacc cttgcaaccc tgaccgaagg ggacatcgtc ggtgaactgg
      601 cgatgctcga cggcgaggtg cgttccgcct cggtcgagac cctgggcggt gacggcgagc
      661 tgctcgccct gcccgcgagc gacgtgcggg ccctgctcga aaggcatccc gacatcaccg
      721 tcaagttcgt cgccgcgctg accaggcgcc tgcgagcggc aaacgaacga atttcccggc
      781 agtccttcca gaccgttcca tcacgggtgg ccggggtcct ttcccagctg gtcgccgagg
      841 agggggagga cggccgccag gtgacgatcc ggatgaatca ggccgacctg gcccagctgg
      901 ccggcacctc ccgcgaaagc gtaagccggt tcctggctga tctggaaagg tcaggcgtgg
      961 tgaaggcagg gcggggccag gtgaccatcc tcgaacccgc ccggctcggc aactacatat
     1021 tctagatcgg gcaaatgtcc ggactcctgg ctctcaactt ctattcccct gtgtttgtcg
     1081 accagctcaa gcggggtcgc aagaccgcca cgatccggct tggcaacaag tccgccaagt
     1141 accagcgcag ccagatcgtg tgggtcacgg tcggttcccg gcactccagg cgggaaaaga
     1201 tcttctccgc ggtgatcgac gaggtcgagg tcaagcggct gtccgaactc tcgccccggg
     1261 acatcgagcg cgacaacccg gaattccgcc gggtcgagga gacgcgggac ttcctccaga
     1321 agatctacga ccgtgagat
//
LOCUS       DAOQFI010000663         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_74675_length_1339_cov_3.288162, whole genome shotgun sequence.
ACCESSION   DAOQFI010000663 DAOQFI010000000
VERSION     DAOQFI010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74675_length_1339_cov_3.288162"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLE93_10330"
     CDS             <1..768
                     /locus_tag="PLE93_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87488.1"
                     /translation="GIGYGEVVCSPYQQWVRFYPSDTSRESAMMTWTYKNWDFGKGYE
                     KAVREAKYTEGTGPDFREGMNKFGPTEKHSTGTFQGIISDRGPIDGPGGVALASPTTF
                     DLDWVWDFNKEGSSYCHVKATFRTQTDQITDPMARSVQIVWRGEANATAKNTSSVVDF
                     PGLGNVQAICQAGPNGYRRLIVDSAIGGSVYTREGSDDYKVNQGVGPLIMRLPNNGML
                     FIQMDNGDRILVSSRWKVNDPVASNNWCVVSAQVYSP"
     gene            complement(869..1021)
                     /locus_tag="PLE93_10335"
     CDS             complement(869..1021)
                     /locus_tag="PLE93_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87489.1"
                     /translation="MLCCRRARGKRFNRGHEFLNPIGQRLNFKADSTYRSTEVIDGGG
                     VRMSGF"
     gene            1076..1327
                     /locus_tag="PLE93_10340"
     CDS             1076..1327
                     /locus_tag="PLE93_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87490.1"
                     /translation="MPELAMAAVAETALRGLGAVRKVASVERSNDQATFVLEVETEGD
                     LITEMRRAMPVPITVDKAPDGSLKVVLEWAWGRSAASAG"
BASE COUNT          260 a          415 c          424 g          240 t
ORIGIN      
        1 ggcatcggat acggcgaagt cgtctgcagc ccgtaccagc agtgggtgcg cttctacccc
       61 tctgatacca gccgcgagag cgcgatgatg acctggacct acaagaactg ggacttcggc
      121 aagggctacg aaaaggccgt gcgtgaagcc aagtacaccg aaggaaccgg gcccgacttc
      181 cgcgagggca tgaacaagtt cggtccgacc gagaagcatt cgaccggcac cttccagggg
      241 atcatctccg accgcggtcc gatcgacgga ccgggcggcg tggccctggc atcaccgacc
      301 accttcgatc ttgactgggt ctgggatttc aacaaggaag gcagctccta ctgccacgtg
      361 aaggcgacct tccgcaccca gaccgatcag atcaccgatc cgatggcccg ttcggtccag
      421 atcgtctggc ggggtgaggc caacgcgacc gcaaagaaca cttcgagcgt cgtcgacttc
      481 cccggactcg ggaacgtgca ggcgatctgc caggccggtc cgaacggtta ccgtcgcctg
      541 atcgtggatt cggcgatcgg cggcagcgtc tacacccggg aaggttccga cgactacaaa
      601 gtcaaccagg gcgtcggtcc gttgatcatg cgcctgccca acaacggcat gctcttcatt
      661 cagatggaca acggagaccg gatcctggtc tcttcgaggt ggaaggtcaa cgacccggtt
      721 gcctccaaca actggtgcgt ggtttccgcc caggtctatt caccttaacg gcgcccggtc
      781 ttcgccgggc accaacggaa gcccgcccgc ccacagcggc aactgattga tgggcgggct
      841 ctggtggaga taggatggga ccgtgagttc agaatcccga catgcggacg ccgccgccgt
      901 cgatgacctc cgtgcttcgg taggtcgagt cagccttgaa gttcaggcgt tggccgatcg
      961 ggttcaggaa ctcgtggccg cggttgaatc tcttgcccct cgccctccgg cagcagagca
     1021 tcccgtcgct tcggctgccc cggcacaagg cgaggtagta gtcagcgttt cccccttgcc
     1081 ggaactggca atggccgcgg tcgccgagac ggcgcttcgc ggactcggtg ctgttcgcaa
     1141 agttgcgtcc gtggaacgat cgaatgatca ggcgacgttc gtgctcgagg tcgaaacgga
     1201 aggcgatttg atcaccgaga tgcgccgggc catgccggtg ccgatcaccg tcgataaagc
     1261 ccccgacgga tcgttgaaag tggttctgga atgggcctgg ggaaggtctg ccgccagcgc
     1321 aggctgagct gatgactct
//
LOCUS       DAOQFI010000664         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_75251_length_1334_cov_3.914777, whole genome shotgun sequence.
ACCESSION   DAOQFI010000664 DAOQFI010000000
VERSION     DAOQFI010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75251_length_1334_cov_3.914777"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLE93_10345"
     CDS             <1..727
                     /locus_tag="PLE93_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HNH87491.1"
                     /translation="DVVLAVAAGSIRKWLVKRGVDTDGLELRAMVPVSVRSDDERGQL
                     GNKLATMRAPLPVYEADPVARLEIVSAAMDGLKQSKQALGAEVIARLNDFAPPTLLAQ
                     AARLTFSTRLFNLLVTNVPGPQIPLYVLGRKLDRPYPVAFLAKNHGLAVGVMSYAGQV
                     NIGLIGDFDAMPDIGQVRRGLNESLAELLAAAEEVENSIAPDKPGKASARKGSARKGS
                     PGKATASNGGGPGRAAKKSSPKG"
     gene            complement(724..1041)
                     /locus_tag="PLE93_10350"
     CDS             complement(724..1041)
                     /locus_tag="PLE93_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNH87492.1"
                     /translation="MAEDHATMPTEEEVFEALEEVIDPELGLDFVSLGLVYDVALEAP
                     DVYVTFTLTTPACPIGPQVSEQMKEFVGGLEGVRDVHPKMIFDPPWSPEMMTDDAKFA
                     LGF"
BASE COUNT          218 a          438 c          435 g          243 t
ORIGIN      
        1 cgacgtggtg ctggcggtcg ccgccggttc gattcgcaag tggctggtca agcgcggggt
       61 tgacaccgat ggccttgagc tgcgggccat ggttccggtc tcggtccgct ccgacgacga
      121 gcgcggccag ctcggcaaca agctcgccac gatgcgggcc ccgctgccgg tttacgaggc
      181 cgatccggtc gcccggctgg agatcgtcag cgccgcgatg gacggcctga agcagtccaa
      241 gcaggcgctg ggggcggaag tgattgcccg actcaatgac ttcgccccgc ccaccctgct
      301 cgcgcaggcg gcccggctga ccttctccac ccggcttttc aatctgctcg tgaccaacgt
      361 tccaggtccg cagattccgc tctatgtcct gggacgcaaa ctcgaccggc cctatccggt
      421 cgccttcctc gccaagaacc acggccttgc ggtcggcgtg atgagttacg ccgggcaggt
      481 caacatcggc ctgatcggtg acttcgacgc gatgcccgac atcggccagg tccgccgcgg
      541 cctgaacgaa tcgctggccg agctgctcgc cgccgccgaa gaggtcgaga actcgatcgc
      601 ccccgacaaa cccgggaagg cttcggccag aaagggttca gcccggaagg gcagccccgg
      661 gaaggccact gccagcaacg gcggcggccc gggccgggcg gcgaagaaga gcagcccgaa
      721 gggctagaag ccgagcgcga acttggcgtc gtcggtcatc atttccggcg accagggcgg
      781 gtcgaagatc atcttcggat gaacgtcgcg tacgccctcg agcccgccga cgaattcctt
      841 catctgctcg gagacctggg gtccgatcgg gcaggcgggc gtggtcaggg tgaatgtcac
      901 gtagacgtcc ggcgcttcca gcgcaacgtc atagacgagc cccagtgaaa cgaagtcgag
      961 cccgagttcg gggtcgatca cttcctcgag cgcttcgaaa acctcttctt cggtcggcat
     1021 ggttgcgtgg tcttcagcca tgaatgcatc ctagctgggt cgggattccg gccgccagcc
     1081 cgtgctgctt cggccggtgg gtcgggtttc cggccggggt gacggaaacc cgacccaccg
     1141 caggctgtaa cccggattgc ccgggtgcgt aacctgtatc tgcccatgtt cgtactgctc
     1201 ctggcattgc tgatcatctg gccgatcgcg gaactcttcg tgatggtgca ggtggccgac
     1261 tggatcggct tcttctggat gcttttcctg ctcttcgcct cgaccgtggc gggcatcatg
     1321 atcctcaaac accg
//
LOCUS       DAOQFI010000665         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_75259_length_1334_cov_3.363565, whole genome shotgun sequence.
ACCESSION   DAOQFI010000665 DAOQFI010000000
VERSION     DAOQFI010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75259_length_1334_cov_3.363565"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLE93_10355"
     CDS             <1..340
                     /locus_tag="PLE93_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH87493.1"
                     /translation="QLLDDVLDIAGPPERTGKARGTDLLDGTVTLPLIFAARFDPALA
                     ALDLRTLNEESAAVACDRIAATGALEEVRSRAIELIEEAKAGLIEAGLEQQQRQLLEL
                     IADGVVQRYS"
     gene            368..934
                     /gene="dcd"
                     /locus_tag="PLE93_10360"
     CDS             368..934
                     /gene="dcd"
                     /locus_tag="PLE93_10360"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNH87494.1"
                     /translation="MVLSDKTLREEIEAGRIVIDPFEEKLIQPSSIDLRVDHSYRVFN
                     NSRYPFIDVKQPMEDLTELVKPSDDEPFILHPGEFVLGQTLERVTVPDDMVARLEGKS
                     SLGRLGLLIHSTAGFVDAGFSGNLTLELSNVANLPITIYKGMPIGQLSFMRMDRAVDT
                     AYGADGKGSKYQGQAEPTASRYYLNFRD"
     gene            996..1319
                     /locus_tag="PLE93_10365"
     CDS             996..1319
                     /locus_tag="PLE93_10365"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNH87495.1"
                     /translation="MIPNIGPLEIVIVLIIALIVFGPKRLPELGKSLGRGLNEFREGM
                     SNIGNHDDDDDEDDAEDHEPAELTPPPHDEKHPLPGEEPEVTEVTTSSDAEPVEGEVV
                     PDSRD"
BASE COUNT          254 a          446 c          415 g          219 t
ORIGIN      
        1 ccagctgctc gacgacgtgc tcgacatcgc cggcccgccg gagcgaaccg gcaaggcccg
       61 gggcaccgac ctgcttgacg gcacggttac cctgccgctg atcttcgccg cccgcttcga
      121 ccccgccctg gccgcgctgg acctccggac cctgaacgaa gaatcggcgg ccgtcgcctg
      181 tgaccggatc gcggccaccg gcgccctgga ggaggtccgg agccgggcga tcgaactgat
      241 cgaggaagcc aaggccggtc tgatcgaagc cggtctcgag cagcagcagc gacagctcct
      301 ggagttgatc gccgatggag tcgtccagag atacagctga ccggctgctc tgggatactg
      361 ccgcttcatg gtcctgagtg acaagaccct gcgggaggag atcgaggccg gacggatcgt
      421 gatcgatccc ttcgaggaga agctgatcca gccctcgagc atcgacctcc gcgttgacca
      481 cagctaccgg gtcttcaaca actcgcgcta cccgttcatc gacgtgaagc agccgatgga
      541 ggatctgacc gagctggtca agcccagcga tgacgagccc ttcatcctcc acccgggcga
      601 gttcgtcctc ggccagaccc tggagcgggt cacggtcccg gatgacatgg tcgcccgcct
      661 ggaaggcaag agctcgctcg gccggctcgg tctcctgatc cattcgaccg cgggtttcgt
      721 cgacgcggga ttctccggca acctgactct cgagctctcc aacgtggcca acctgccgat
      781 cacgatttac aagggcatgc cgatcggcca gctctccttc atgcggatgg accgggcggt
      841 tgacacggct tacggagccg acggcaaggg ttcgaagtac cagggccagg ccgaacccac
      901 cgcctcgcgc tactacctga atttccggga ctgaactcct tcaccgcccg gtgatccggg
      961 cccgccggcg gttcgtaggt acgattggtg tccagatgat tccgaatatt ggcccgctcg
     1021 aaatagtcat cgtcctgatc atcgcgctga tcgttttcgg cccgaagcgc ctgcccgaac
     1081 tcggcaagtc gctgggccgg ggtctcaacg agttccgtga aggcatgagc aacatcggaa
     1141 atcacgacga tgacgacgat gaagatgacg ccgaggatca cgaaccggcc gaactgaccc
     1201 cgcccccgca tgacgagaag catccgcttc caggcgaaga gcccgaggtc accgaggtga
     1261 ccaccagctc cgacgccgaa ccggtcgagg gtgaagtggt tcccgactca cgggactgag
     1321 gccgctgtgg ccag
//
LOCUS       DAOQFI010000666         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_75276_length_1334_cov_2.869429, whole genome shotgun sequence.
ACCESSION   DAOQFI010000666 DAOQFI010000000
VERSION     DAOQFI010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75276_length_1334_cov_2.869429"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLE93_10370"
     CDS             complement(<1..534)
                     /locus_tag="PLE93_10370"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HNH87496.1"
                     /translation="MKGRILVMGGHEFDRLDGNEAIVEHVISLTGMKAPKICLLPTAS
                     GDPEDQISRFRRSFGSRGCEVSDISLFRLGANPIDVSAHLMKQDAIYVGGGSLVNLVA
                     VWKPHGIAGLIEECLERGVMVVGQSAGAMCWFEAGITSSSGRPEPAEGLGVLKGSLCV
                     HYHRDPERRTRYLAEVGK"
     gene            complement(569..1291)
                     /locus_tag="PLE93_10375"
     CDS             complement(569..1291)
                     /locus_tag="PLE93_10375"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HNH87497.1"
                     /translation="MESGNPLLDDFVVQATGLKKPRVCFMPTASGDADHYLVRFYNAF
                     RGRGEASHISLFRRERGAESIRDHLLSQDLIYVGGGSVISMLGVWRAHGIDRILREAW
                     ERGIVLCGLSAGSLCWFEECVTTYQKETRVTIGLGLLPWSNQVHFELGSTAHKAFEEK
                     LKEGLRPGYAATDGAAFHFIDTELAQVVASRPEARAYRIELKGDHPKTTQLATRYLGS
                     PDQVPLVEESPIPWASTPIEAA"
BASE COUNT          234 a          443 c          430 g          227 t
ORIGIN      
        1 cttgccgacc tcggccaggt agcgggtccg ccgttccgga tcccggtggt aatggacgca
       61 gaggctgccc ttcagcacac cgagaccctc cgccggctcg ggtcgccccg aggacgaggt
      121 gatgccggcc tcgaaccagc acatcgcgcc ggcactctgt cccaccacca tcacgccgcg
      181 ctcgaggcat tcttcgatca gcccggcgat tccatgtggc ttccagaccg cgaccaggtt
      241 gaccaggctg cccccgccga cgtagatcgc gtcctgtttc atcaggtgcg cggaaacatc
      301 gatcggattc gcaccgagcc ggaacagcga gatgtcactg acctcgcaac cgcgactgcc
      361 gaaggaccgg cgaaagcggg agatctggtc ttccgggtca cccgatgcgg tcggcagcag
      421 gcagatcttt ggcgctttca tcccggtcag cgagatgacg tgctcgacga tcgcctcgtt
      481 gccatcgaga cggtcgaact cgtgaccacc catgaccagg atccggccct tcatcgcaga
      541 acccgcttgc ggtttcggtt gcctcgcatc aggccgcttc gatcggggtt gacgcccagg
      601 gaatcggtga ctcctcgacc agcggcacct ggtcgggtga gccgaggtac ctcgtcgcga
      661 gctgggttgt cttggggtga tcgcccttga gttcgatccg gtaggcccgg gcctcgggcc
      721 gggaagccac gacctgcgcg agttcggtgt cgatgaagtg aaaggcggca ccatcggtcg
      781 ccgcgtaccc cggacgcagg ccttccttca gcttttcctc gaacgccttg tgggcagtcg
      841 agcccagttc gaagtgaacc tggttcgacc agggcagcag accgaggccg atcgtcaccc
      901 tggtttcctt ctggtaggtg gtcacgcatt cctcgaacca gcagagcgag ccggctgaaa
      961 gcccgcagag gacgatgccg cgctcccagg cctcgcggag aatccggtct atgccgtggg
     1021 cgcgccagac cccgagcatg ctgatcacgc tgccaccgcc gacgtagatc aggtcctggg
     1081 agagcaggtg gtcacggatc gattccgccc cgcgctcccg ccggaagaga gagatgtgcg
     1141 aggcctcgcc gcggccccga aaggcgttgt agaagcggac caggtaatgg tcggcgtcgc
     1201 cggaagcggt cggcatgaag cagacccggg gcttcttcag gccggtcgcc tgcaccacga
     1261 agtcatcaag caggggattg ccggattcca tcgagaaccc gcgcgtcgtg gtgctgacgc
     1321 caggccgcta caac
//
LOCUS       DAOQFI010000667         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_75914_length_1328_cov_2.865672, whole genome shotgun sequence.
ACCESSION   DAOQFI010000667 DAOQFI010000000
VERSION     DAOQFI010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75914_length_1328_cov_2.865672"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLE93_10380"
     CDS             complement(<1..569)
                     /locus_tag="PLE93_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNH87498.1"
                     /translation="MKVGVIKEIKQDEYRVAITPAGVREFVEHGHEVIIEDGAGEGSA
                     IPNTSYEAQGARILPDASSVFEAAEMILHVKELQASEIEMLKPGHLLFTYLHLAPDPE
                     QTAGLMKSGSTCVAYETVEDAEGRLPLLAPMSEIAGRIATQAGAFMLEKPLGGRGVLL
                     GGVPGVAAANVVVIGGGVVGTNAAFIAMG"
     gene            complement(641..>1328)
                     /locus_tag="PLE93_10385"
     CDS             complement(641..>1328)
                     /locus_tag="PLE93_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH87499.1"
                     /translation="GCFVPPDGYFQRVREICDKYDVLLISDEVICAWGRLGHYFGCER
                     FGYQPDIITSAKALTSSYVPMGAMIASDKVAEPFMEGTASFAHGFTFAGHPVGAAVAL
                     ANIEIFEREDLCGHVLAKEGEFRGMLESLRDIPIVGDVRGAGYFQAIELVKDQETKQS
                     FNDEESEKLLRGFLSGALYERGLICRADDRGDPVIQFSPPLICDTEQFEEIHDVLRPV
                     LTEAAAMMRN"
BASE COUNT          213 a          437 c          437 g          241 t
ORIGIN      
        1 atgcccatcg cgatgaaagc ggcattggtg ccgaccacgc cgccaccgat gaccacgacg
       61 ttggccgccg cgactccggg cacgccgccg agcagcacgc cccggccccc cagcggcttc
      121 tccagcatga aggcgccggc ctgggttgcg atccggccgg cgatctcgct catcggcgcc
      181 agcagcggca gccggccctc ggcgtcttcg accgtctcat acgcgacgca ggtcgagccg
      241 gacttcatca ggccggcggt ctgctcgggg tcgggggcga ggtgcaggta ggtgaagagc
      301 agatgcccgg gcttcagcat ctcgatctcg gatgcctgca gttccttcac atgcaggatc
      361 atctcggccg cctcgaagac ggaactggcg tcgggcagga tccgcgcacc ctgcgcctcg
      421 tatgaggtat tcgggatggc gctgccttcg ccggcgccgt cctcgatgat tacctcgtgg
      481 ccgtgctcga cgaattcacg caccccggcc ggcgtgatcg ccacccggta ctcgtcctgc
      541 ttgatctcct tgatgacgcc gaccttcatc tcttacctcg tcctctctgg tggtccgctg
      601 gggtgacttt tccgccgggc ggggtgaatg ggtgaatcga tcagttcctc atcatggccg
      661 cggcctcggt gaggaccggc ctgaggacat catgaatttc ctcgaactgc tcggtgtcgc
      721 agatgagcgg cggcgagaac tggatcacgg ggtctccccg gtcgtcggcc cggcagatca
      781 ggccgcgttc gtagagcgcg cctgaaagga agccgcgcaa cagcttctct gactcttcgt
      841 cgttgaagct ctgcttcgtt tcctggtcct tgaccagctc gatcgcctgg aagtaaccgg
      901 cgccgcgcac gtcaccgacg atcgggatgt cccggagcga ttccagcatg ccgcggaatt
      961 cgccctcctt tgccagcacg tggccgcaga ggtcctcgcg ttcgaagatc tcgatgttgg
     1021 ccagggcgac cgccgcgccg accgggtggc cggcgaaggt gaagccgtgc gcgaaggagg
     1081 cggtgccctc catgaacggt tcggcgacct tgtcggaggc gatcatcgcg cccatcggca
     1141 cgtaggagga ggtcagggcc ttggccgagg tgatgatgtc cggctggtag ccgaaacgct
     1201 cgcagccgaa gtagtggccg agccggcccc aggcgcagat cacttcgtcg gagatcagca
     1261 gcacgtcgta cttgtcgcag atttcgcgga cccgctggaa gtaaccgtcg ggcggaacaa
     1321 agcagccg
//
LOCUS       DAOQFI010000668         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_76418_length_1324_cov_2.684791, whole genome shotgun sequence.
ACCESSION   DAOQFI010000668 DAOQFI010000000
VERSION     DAOQFI010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76418_length_1324_cov_2.684791"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLE93_10390"
     CDS             <1..853
                     /locus_tag="PLE93_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNH87500.1"
                     /translation="GGLIGSAIAFVLLALRNRGYRRFETAIIGLLGIILAGFLYEAFK
                     IGPDASGVMGGFVPQLQGTDSLLLAVGIIGATVMPHVIYLHSALTQDRIQPRDDKERH
                     SLIDFQKIDVGLAMGIAGFINMSMLAIAAAVLGGTGIDSIEGAFDGLKDLVSPAAGIA
                     FGLALLASGIAASSVGTFAGQVVMQGFINRMIPLFLRRAITMAPALIILAIGLDPTRS
                     LVISQVILSFGIPFALVPLIVLTSRRDVMGEGFENHRLTTIVASIFAAIIIALNLFLL
                     FQTFTGN"
     gene            1016..>1324
                     /locus_tag="PLE93_10395"
     CDS             1016..>1324
                     /locus_tag="PLE93_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH87501.1"
                     /translation="MALYVLCAGVLMIVLDATVVNVALPAIQDDLGFSQSSLAWVVNA
                     YLIAFGGLLLLSGRLGDLISRRDVFLVGLVVFTTASLLCGFAQSDVMLVAFRFLAGIG
                     G"
BASE COUNT          190 a          469 c          391 g          274 t
ORIGIN      
        1 cggcggcttg atcggcagcg caatcgcctt cgttctcctc gcccttcgca accgcggcta
       61 ccggcgtttc gagaccgcga tcatcggcct gctcgggatc atcctggccg gctttctcta
      121 cgaggccttc aagatcggcc cggatgcttc cggggtgatg ggcggattcg ttccgcagct
      181 gcagggcacc gacagcctgc tgctcgcggt cgggatcatc ggggcgacgg tcatgcccca
      241 tgtgatctac ctgcattcgg ccctgaccca ggaccgcatc cagccgcggg atgacaagga
      301 gcggcactcc ctgatcgact tccagaagat cgacgtcgga ctggcgatgg gcatcgccgg
      361 cttcatcaac atgtcgatgc tggcgatcgc cgcggccgtg ctcggcggca ccggcatcga
      421 ctcgatcgaa ggcgccttcg acggcctcaa ggatctggtc agccccgccg ccggtatcgc
      481 cttcggcctg gccctgctcg cctcgggcat cgccgcctcc agcgtcggca cctttgccgg
      541 gcaggtggtc atgcagggct tcatcaaccg gatgatccct ctcttcctgc ggcgggcgat
      601 cacgatggcc cccgccctga tcatcctcgc gatcggcctc gacccgaccc gctcgctggt
      661 gatcagccag gtcatcctct ccttcggcat tcccttcgcg ctggtgccgc tgatcgtgct
      721 cacctcgcgc cgcgacgtaa tgggcgaggg ctttgaaaac caccggctga ccacgatcgt
      781 cgcctcgatc ttcgccgcga tcatcatcgc gctcaacctc ttcctgctct tccagacctt
      841 caccggtaac tgaggccacc ggaccggacg gaccgtccgc ggcggcccgg gccgaacggc
      901 cacttttgtt agtctcagcc gcctgaccga ccagtctgaa aacacggtaa ttccatccga
      961 ttcagactcc gataaccagc ccgaccatcc gaaggatccg gatcgttccc gctggattgc
     1021 gctctacgtc ctgtgcgccg gggtattgat gatcgttctc gacgcaaccg tcgtgaacgt
     1081 cgccctgccg gcaatccagg acgatctcgg gttttcccaa tccagcctgg cctgggtggt
     1141 caacgcgtac ctgatcgcct tcggcgggct gctgctcctc tcggggaggc tgggcgacct
     1201 gatctcccgg cgcgacgtgt tcctggtcgg cctggtcgtt ttcaccaccg cgtccctact
     1261 ttgcggtttc gcccagagtg atgtgatgct ggtcgccttc cgtttcctgg ccgggatcgg
     1321 gggg
//
LOCUS       DAOQFI010000669         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_76526_length_1323_cov_3.027603, whole genome shotgun sequence.
ACCESSION   DAOQFI010000669 DAOQFI010000000
VERSION     DAOQFI010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76526_length_1323_cov_3.027603"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLE93_10400"
     CDS             complement(<1..564)
                     /locus_tag="PLE93_10400"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNH87502.1"
                     /translation="MSTLLTCLAILVAGCLVTGISILLVRKFVPEHLRGSEERREPVF
                     AFAGVLYALVIGFVLAYALDGYQTAETAAGTEADSVSSLSRAATLFDAESRDRIGHEL
                     ICYSRAVVDDEWPAMEHGRRSELATAATDRLFQSFGKLGRNSADNSALSTSLERVSDL
                     QKARATRLLKSDTFLPTMFWVFMVVGGF"
     gene            complement(564..>1323)
                     /locus_tag="PLE93_10405"
     CDS             complement(564..>1323)
                     /locus_tag="PLE93_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87503.1"
                     /translation="VPNDNVQAAALALFMDQESVNEVFVVDDGDTYGEGLRFRFEKVA
                     RHTGIEITGGATVTRSIQIGDVAKRVIGSGADALLFAGSNLDLARDLFETVHREDNFI
                     KLFGGDGLSLSSFLGSLGDTGLDTYITAPQLPAGNYARSGESFLRGFQERYGRPAEPM
                     AVFAYEAGHVVIDSIRKGTRGDIATEPIDALRQNTRNAFFDTSERASPLGSYSIDTNG
                     DTTLTFYGAYRVEDGQLVLGRTIDVPPSLLREDE"
BASE COUNT          250 a          398 c          458 g          217 t
ORIGIN      
        1 gaagccgccg accaccatga agacccagaa catcgtgggc aggaaggtgt cggatttcag
       61 cagcctggtg gcccgggcct tttgcaggtc gctgacccgc tccagggagg ttgaaagggc
      121 cgagttgtcg gcgctgtttc gtcccagctt gccgaaggac tgaaagagcc ggtcggtggc
      181 ggcggtcgcg agctcggaac ggcggccatg ctccatcgcc ggccactcgt catcgacaac
      241 ggcgcgggag taacagatca gttcgtggcc gatccggtcc cggctctcgg catcgaaaag
      301 agtagccgcg cgggaaaggg aactgaccga atcggcttcg gtgccggctg cggtctcggc
      361 ggtctggtag ccgtcgagcg cgtaggcgag gacgaaaccg atcaccaggg cgtagagcac
      421 gccggcgaaa gcgaagacgg gctcgcggcg ctcttcgctg cccctcaggt gttcggggac
      481 gaacttgcgg accagcagga tcgagatccc ggtgaccagg catcccgcca cgaggatcgc
      541 caggcaggta agcagggtgc tcatcactcg tcctcccgaa ggagagaagg tggcacgtcg
      601 atcgtccggc cgagcaccag ctggccgtcc tcgacccggt aggcgccgta gaaggtaagg
      661 gtggtgtcac cgttcgtgtc gatcgaatag ctgccgagcg gcgaggcgcg ctcggaggtg
      721 tcgaagaagg cgttgcgggt gttctgccgg agcgcgtcga tcggttcggt tgcgatgtcg
      781 ccgcgggtgc ccttgcgaat cgaatcgatc acgacatgac ctgcctcata ggcgaagacg
      841 gccatcggct cggccggtcg tccataccgt tcctggaaac cacgcaggaa ggattcaccg
      901 ctccgcgcgt aattgccggc cgggagctga ggcgccgtga tgtacgtgtc aagcccggtg
      961 tcaccgagcg atccgaggaa ggaggaaagc gagagaccgt caccaccgaa gagcttgatg
     1021 aagttgtcct cgcggtgcac cgtctcgaag aggtcacggg ccagatcaag gttcgacccg
     1081 gcgaagagca gggcgtcggc cccggatccg atgactcgtt tcgcgacgtc gccaatctgg
     1141 atcgaacggg taacggtcgc gccgccggtg atctcgatcc cggtgtgccg ggcgaccttc
     1201 tcgaagcgga aacgcagtcc ctcgccgtag gtgtcgccgt catcgaccac gaacacctcg
     1261 ttgacgctct cctgatccat gaacagggcc agggccgcgg cctggacatt gtcattggga
     1321 acc
//
LOCUS       DAOQFI010000670         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_76644_length_1322_cov_2.688240, whole genome shotgun sequence.
ACCESSION   DAOQFI010000670 DAOQFI010000000
VERSION     DAOQFI010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76644_length_1322_cov_2.688240"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLE93_10410"
     CDS             complement(<1..527)
                     /locus_tag="PLE93_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87504.1"
                     /translation="MTPSLYETSNLLPRSRFLLLALTVAALLLPSAASPGQSQAATWT
                     VRGGGFGHGLGMSAYGAYGMGLDGYRYGQILRHYYRGIRIRKLRAKRNIKVLLDVDAT
                     PYFSGARWACGRKLKPRLTYGAALGRNGIFLRGAGGKPLKKCGGRLRTEASGNVVFKG
                     LGAYRGGVEIVRGGGS"
     gene            642..>1322
                     /locus_tag="PLE93_10415"
     CDS             642..>1322
                     /locus_tag="PLE93_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH87505.1"
                     /translation="MDHKLLLAGEWVETGVWDEVLSPWDQSPIARVAQGDEGTVDKAV
                     KAAHKVFTESPIPQYERAEILDRAAHLIRERQEDLAQTISAEAGKPIKTARVEASRAA
                     DTMTFSAVEARKLTGGTVPMEASPAGVGKLGVMLRVPLGVVGAISPFNFPLNLVCHKL
                     GPAIAAGNAVVLKPAGQTPVSSIKLAEVLIDAGLPAGWLSVIPGGGSQVGNAIVEHEL
                     TAAISFTGS"
BASE COUNT          231 a          444 c          444 g          203 t
ORIGIN      
        1 gaaccgccgc cgcgtactat ctcgaccccg ccgcggtagg cgccgagccc cttgaagacg
       61 acgttgccgc tggcctcggt ccggagccgg ccgccgcact tcttcaacgg cttgccgccg
      121 gcgccccgga gaaagatgcc gttgcggccg agcgccgccc cgtaggtgag gcgtggtttc
      181 agcttgcggc cgcaggccca acgggcgcca ctgaagtacg gggtcgcgtc aacgtcaagc
      241 aggaccttga tgtttcgctt cgccctgagc ttgcggatcc ggatgccgcg gtagtagtgg
      301 cgaaggatct ggccgtacct gtagccgtcc agccccattc cgtaggcgcc gtaggcgctc
      361 atgccgaggc catggccgaa tccgccaccg cgaacggtcc aggtcgcagc ctgggactgg
      421 ccgggtgaag ccgcggaagg cagcagcaga gcggcaaccg tcagcgccag caacaggaag
      481 cgcgaacgcg ggagaagatt tgacgtttcg tacagactgg gggtcatgcc ttgtcacaac
      541 aggttcgagg ggggaaggtc gcggcctgcc attatctttg gaaggagtct tgccccgggg
      601 ccggaccagc cccacagatt gccacatcga aggagaagcg agtggatcac aagttgctgt
      661 tggcaggtga atgggtcgaa accggcgtct gggacgaagt gctcagcccc tgggatcaga
      721 gcccgatcgc ccgcgttgcc cagggtgacg aaggcaccgt cgacaaagcc gtgaaggccg
      781 cccacaaggt cttcaccgag tcacccatcc cccagtacga gcgggccgag atcctcgacc
      841 gggccgcaca tctgatccgg gagcggcagg aagacctggc ccagacgatt tcagccgaag
      901 cgggcaagcc gatcaagacc gcccgggtcg aggcttcacg ggccgccgac acgatgacct
      961 tctccgcggt cgaggcccgc aagctgaccg gtggcacggt gccgatggaa gcctccccgg
     1021 ccggggtcgg caagctcggt gtgatgctgc gggtgccgct tggcgtggtc ggcgcgatca
     1081 gccccttcaa cttccccctc aacctggtct gccacaagct cggtccggcg atcgccgccg
     1141 gcaatgcggt ggtcctcaag ccggccggcc agactccggt ctcctcgatc aagctggccg
     1201 aggtgctgat cgacgccggc ctgcccgccg gctggctcag cgtcatcccc ggcggcggtt
     1261 cccaggtcgg caacgcgatc gtcgagcacg aactgaccgc ggcaatctcc ttcaccggtt
     1321 cg
//
LOCUS       DAOQFI010000671         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_76850_length_1320_cov_3.090119, whole genome shotgun sequence.
ACCESSION   DAOQFI010000671 DAOQFI010000000
VERSION     DAOQFI010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76850_length_1320_cov_3.090119"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLE93_10420"
     CDS             complement(<1..410)
                     /locus_tag="PLE93_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNH87506.1"
                     /translation="MALTSEQKTAIDCDDRDILLQAGAGSGKTSTTVNRYERLLTGVR
                     TDGRTSEVLEPSAILVFTFTDKAAGELRERIRRLRESGGREFSMGSIWVGTFHSICAR
                     ILRAHPVAAGVDPAFDVIDDVVAARLKESAYEAGL"
     gene            complement(401..>1320)
                     /locus_tag="PLE93_10425"
     CDS             complement(401..>1320)
                     /locus_tag="PLE93_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNH87507.1"
                     /translation="AAAALAERNVFSATALEAFIECPYRWFFERAMNPVRFGPEPEPL
                     AKGNLVHEVLAALYSSRRATIPNGDDIEDWIAAARTLVDELSGQCDLGGDSARHRIQR
                     RQVSIEIERFLRREAERETAFRPVDLERMFGFEREDSLPPLEFDGWKLRGKIDRIDQD
                     ATGRGIVFDYKSGSSSYKSRAELKREGKVQLPLYLRALEVLWDLDPAAGLYVPIFAAK
                     HQPRGLVSEAASPDLKDLNPVSKDFVEDLPEEIQQDVEQAAEAAWKILHGQIAHDPTE
                     CINHFSHAGVPDWNPDVEENFTGATFRWP"
BASE COUNT          231 a          433 c          408 g          248 t
ORIGIN      
        1 agccccgcct cgtatgcgga ctccttgagc cttgcggcga ccacgtcatc gatcacgtcg
       61 aaagccggat cgaccccggc ggcgaccgga tgtgcccgga ggatgcgggc acagatcgag
      121 tgaaaggtcc cgacccagat cgaacccatc gaaaattcac ggccgcccga ttccctgagg
      181 cggcgaatgc gctcccgaag ctcccccgcg gccttgtcgg tgaaggtgaa gaccaggatc
      241 gccgacggct ccagaacctc ggaagtcctg ccatcggtcc gaactccggt cagcagccgc
      301 tcgtaacgat ttacggtcgt gctcgtcttg cccgaaccgg caccggcctg cagcaggatg
      361 tcccggtcgt cgcagtcgat cgccgttttc tgttccgaag tcagggccat cgaaaggtcg
      421 ctccggtgaa gttctcttcg acgtcggggt tccagtcggg aactcccgca tgcgagaagt
      481 gattgatgca ctcggtcgga tcgtgcgcga tctggccgtg gagaatcttc caggcggctt
      541 cggcagcctg ttcgacatcc tgctggatct cctccggcag atcttccacg aagtccttcg
      601 aaaccggatt cagatccttc aggtcggggc ttgccgcttc ggaaaccagt cctcgcggct
      661 ggtgcttcgc ggcgaagatc ggcacgtaga gtccggccgc agggtccagg tcccagagca
      721 cttcgagggc ccgaaggtag agcgggagct ggaccttgcc ttcgcgtttc agttcggccc
      781 gcgacttgta ggaagacgag cctgacttgt agtcgaagac gatcccccgt ccggtggcgt
      841 cctggtcgat ccggtcgatc ttccccctca gtttccagcc gtcgaactcg agcggcggca
      901 gcgaatcctc cctttcgaag ccgaacatcc gctccagatc gaccggcctg aaagcggttt
      961 cccgttccgc ctcgcggcgc aggaatcgtt cgatctcgat cgagacctgc cggcgctgaa
     1021 tccggtgtcg ggccgaatct ccgcccaggt cgcattgccc ggacaattcg tccacgagcg
     1081 ttcgcgccgc ggcgatccag tcctcgatgt cgtcgccatt cgggatcgtt gcccgacgcg
     1141 aggagtagag agcggccaga acctcgtgca cgaggttgcc cttcgccagg ggttcgggct
     1201 ccggtccgaa tcgcaccgga ttcatcgccc gctcgaagaa ccatcggtag ggacattcga
     1261 tgaacgcctc aagggcggtc gccgagaaaa cgttccgttc ggcgagcgcg gcagcagccg
//
LOCUS       DAOQFI010000672         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_77113_length_1318_cov_2.534442, whole genome shotgun sequence.
ACCESSION   DAOQFI010000672 DAOQFI010000000
VERSION     DAOQFI010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77113_length_1318_cov_2.534442"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLE93_10430"
     CDS             complement(<1..732)
                     /locus_tag="PLE93_10430"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH87508.1"
                     /translation="MQAGSKLKIEGLSKTYRGAGGGENVTALKDVGIEIGAGEFVSIV
                     GPSGCGKSTLFNIIAGLIRPSSGKVLLDGRQPDELLGSVGYMPQRDCLMPWRTVLDNT
                     TLGLELNGVRKKEAHKKALAEFERFGLAGFEKHWPAKLSGGMKQRAALLRTFLAGRDV
                     MLLDEPFGALDALTRANMQQWLLDVWQEDRKTILFVTHDVEEAIYLSDRVYVMSGRPG
                     RVSLDVNVNLERPRKPEITMSPEFLV"
     gene            complement(765..>1318)
                     /locus_tag="PLE93_10435"
     CDS             complement(765..>1318)
                     /locus_tag="PLE93_10435"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH87509.1"
                     /translation="NAILNTIMKDAGGNPDDANVVTIGFNGVQSLEAGKVSAFTGFIP
                     ADGVQVEADGYPTKSFPLDENGGPSYPGLVVFSTESKIGDDPDLMQGFVDATTKGYQD
                     ALADPQAALSALLAQTQGLDKGLQEKVFDAYIPYFGPADTFGQFDKANLESLSTFMVD
                     NDLASEPISPDRYATTDFTQPKE"
BASE COUNT          221 a          413 c          426 g          258 t
ORIGIN      
        1 gaccaggaac tcgggtgaca tcgtgatctc aggcttgcgt ggccgttcca ggttcacgtt
       61 cacgtcgagg gagacgcgac ccggccggcc ggacatcacg tagacccggt cggagaggta
      121 gatcgcctcc tcgacgtcat gggtcacgaa aagaatggtc ttgcggtcct cctgccagac
      181 gtccagcagc cactgctgca tgttggcccg ggtcaacgcg tccagggcgc cgaagggctc
      241 gtcgagcagc atcacgtcac ggccggcgag gaaggtgcgc agcagggcgg cacgctgttt
      301 catgccgccg gagagcttgg ccggccagtg cttctcgaag ccggccaggc cgaagcgctc
      361 gaactcggcc agcgctttct tgtgcgcttc cttcttgcgc acgccgttca gctccagccc
      421 gagcgtcgtg ttgtcgagca ccgtgcgcca gggcatgagg cagtcgcgct ggggcatgta
      481 cccgaccgag ccgagcagtt cgtcgggttg ccggccgtcg agcagcacct tgccgctcga
      541 cggccggatc agcccggcga tgatgttgaa gagggtcgac ttgccacatc cagagggacc
      601 cacgatcgag acgaattcgc cggctcctat ttcgatgccg acatccttca gcgcggtgac
      661 gttctcgccg ccaccagcgc cccgataggt cttcgataga ccttcgatct tgagtttcga
      721 acctgcctgc aagtcaatcc tcctttgctt ctcttacgcc ctgatcactc tttgggctgg
      781 gtgaaatcgg tggtcgcgta acggtccggc gagatcggtt cgctggccag gtcgttgtcc
      841 accatgaacg tggagagtga ctccaggttc gccttgtcga actggccgaa ggtgtcggcc
      901 gggccgaagt aggggatgta ggcgtcgaac accttctcct gcagcccctt gtcgagaccc
      961 tgggtctggg ccagcagggc gctcagcgcg gcctgcggat cagccagggc atcctggtat
     1021 cccttggtgg tcgcgtcaac gaagccctgc atcaggtcag ggtcatcacc gatcttcgac
     1081 tcggtcgaga agacgaccag gcccgggtag gaaggaccgc cgttctcgtc cagggggaac
     1141 gacttggtcg ggtaaccgtc cgcttcaacc tggacgccgt cagccgggat gaacccggtg
     1201 aaggccgaga ccttgccggc ttccagcgac tgaacgccgt tgaatccgat cgtgaccacg
     1261 ttggcgtcgt cgggattgcc gccggcgtcc ttcatgatcg tgttcaggat cgcgttgt
//
LOCUS       DAOQFI010000673         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_77208_length_1317_cov_3.198098, whole genome shotgun sequence.
ACCESSION   DAOQFI010000673 DAOQFI010000000
VERSION     DAOQFI010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77208_length_1317_cov_3.198098"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLE93_10440"
     CDS             complement(<1..246)
                     /locus_tag="PLE93_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87510.1"
                     /translation="MGKQADQWQQIVSSYKGAGEVKWEPVGGINPVGAPVALCVGGSN
                     RLTGELAPDFWGASCDADEEEVGGFMRKELVPTAILAK"
     gene            330..896
                     /locus_tag="PLE93_10445"
     CDS             330..896
                     /locus_tag="PLE93_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNH87511.1"
                     /translation="MSGALIALIVVVVIALIAVLWYIATRNGIIASRNRVEESWSGID
                     VQLKRRHDLVPNLVETVKGYATHEQKVFEEVTKARADAMKAQSVDETAQAESGLNRSL
                     ADLRAVAEQYPDLKATENFQQLSRNLSELEDEIQASRRIYNSNVQAYNTKIQQFPASV
                     VANQGGFTAKEFFEIEDAAERATPNVSF"
     gene            complement(956..>1317)
                     /locus_tag="PLE93_10450"
     CDS             complement(956..>1317)
                     /locus_tag="PLE93_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87512.1"
                     /translation="AGEAATYDEEVRELWSTVLETTTEGTREEIERLQKAGSVNESLD
                     PEALAEGLVLMTERSFQVHLAQGEDDPNEVASSLTRVWWATLFGPPLPSSPPEGEGGP
                     QGSQPGPEGRGMGDPEK"
BASE COUNT          237 a          451 c          401 g          228 t
ORIGIN      
        1 cttcgcgagg atcgcggtcg ggaccagttc cttgcgcatg aatccgccga cttcctcctc
       61 gtcggcgtcg cagcttgctc cccagaagtc cggggcgagc tcgccggtca gccggttgct
      121 gccgccgaca caaagcgcga ccggtgcgcc gaccgggttt atgccgccaa ccggctccca
      181 tttcacctcg cccgccccct tgtaggaaga gacgatctgc tgccactggt cagcttgctt
      241 gcccatcgcc gatcacccta cccgtagtca cagccgaatc aggcggatag gatggccgcg
      301 atactcattt caaccgagga gacttgccta tgagtggcgc acttatcgcg ctgatagtgg
      361 tcgtcgtaat cgccctgatc gcggtgctct ggtacatcgc gacgcggaac ggaatcatcg
      421 cttcgcgcaa ccgggtcgag gaatcgtgga gcggcatcga cgtccagctg aagcgccgac
      481 atgacctggt cccgaacctg gtcgagacgg tcaagggcta cgcgactcac gagcagaagg
      541 tcttcgagga agtcaccaag gcccgtgccg atgcgatgaa ggcccagtcc gtcgacgaga
      601 ccgctcaggc ggaatccggg ctcaaccgct cgctcgccga cctgcgtgcc gtggccgagc
      661 agtacccgga cctgaaggcg accgagaact tccagcagct cagccggaac ctctcggaac
      721 tcgaggacga gatccaggct tcccgccgga tctacaactc gaacgtccag gcgtacaaca
      781 cgaagatcca gcagttcccc gcctccgtcg tcgccaatca gggcggcttc acggccaagg
      841 agttcttcga gatcgaggac gccgccgagc gcgccacccc caacgtcagc ttctgagggg
      901 gcacggcatg ggggaccccg aaaagtaacg agcgctccgc gctctgatta aggagtcact
      961 tttcggggtc ccccatcccg cgcccttccg gaccgggctg ggacccctgc gggccacctt
     1021 cgccctccgg gggtgaggag ggtaaagggg gaccgaagag agtggcccac cagactcggg
     1081 tcaggctcga ggcgacctcg ttcgggtcgt cctcgccctg ggcaaggtgg acctggaagc
     1141 tcctttcggt catcagcacc agaccttcgg caagtgcctc cggatccagt gattcgttga
     1201 ccgagcctgc cttctgcaac cgctcgatct cctcccgggt gccttcggtg gtcgtttcca
     1261 gcacggtcga ccagagctcg cgcacctctt cgtcgtaggt ggccgcctcg ccggcca
//
LOCUS       DAOQFI010000674         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_77333_length_1316_cov_2.425852, whole genome shotgun sequence.
ACCESSION   DAOQFI010000674 DAOQFI010000000
VERSION     DAOQFI010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77333_length_1316_cov_2.425852"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLE93_10455"
     CDS             complement(<1..342)
                     /locus_tag="PLE93_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNH87513.1"
                     /translation="MTLVVKLGSSIVSADDGSLREEVLDSVCAQAAALRRAGEPLVMV
                     TSGSIARGIRLLGLQARPRAIDELQAASAVGQGSLFREYESRLETGEAAAAQVLLTAS
                     DVANRTSYVNAR"
     gene            complement(468..>1316)
                     /gene="cgtA"
                     /locus_tag="PLE93_10460"
     CDS             complement(468..>1316)
                     /gene="cgtA"
                     /locus_tag="PLE93_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HNH87514.1"
                     /translation="ELRLKLLADAGLIGLPNAGKSSLLSRLTRADPKIADYPFTTLEP
                     VLGTIETPDRQVVIADIPGLIEGAAEGAGLGHEFLAHVERCAMLIHVVELAPSDGEPL
                     ENYRAVRAELAAHSTGLEKLPELLVLSKSDLWTEADIADQVEEWDRELGESVLGIIPV
                     SSATGAGLERLKESIVRFVPDQVEAPVVTSADGIEVDFEAEHLTYRPKGEGGYSVEEE
                     EEGAFRIHGHGIEVLFERFDTGNEEALAYLESRLTEIGVMAELKRAGFESGDEIRVGD
                     LEFELY"
BASE COUNT          219 a          447 c          432 g          218 t
ORIGIN      
        1 gcgggcgttc acgtaactgg tccggttggc aacgtcggat gcggtcagca gcacctgggc
       61 ggccgctgcc tcccccgtct cgagccggga ctcgtactcg cggaagagcg agccctggcc
      121 gaccgcggaa gcggcctgca gctcgtcgat cgcccgcggc ctggcctgga gaccgagcag
      181 ccggatcccc cgggcgatcg acccggaggt gaccatgacc agcggctccc cggcccgccg
      241 cagagcagcc gcctgggcgc agacggaatc gagcacctcc tcgcgcaggc tcccgtcatc
      301 cgccgaaacg atcgaagatc caagtttcac caccaaagtc atctccccac caaccctaga
      361 ggctgatgcc acgaccggca gagcccctcc caaccatcct ttgctcctga tgggaggggc
      421 gggtggagat gggagggccg gcgaagaccg gcccgactgc agttaagtca gtaaagctcg
      481 aactcgaggt ctccgacccg gatctcgtct ccggactcaa acccggcccg tttcagttcg
      541 gccatcacgc cgatttcggt gagacgggat tcgaggtagg cgagtgcttc ctcgttgccg
      601 gtgtcgaagc gttcgaagag aacctcgatg ccgtggccgt ggatccggaa ggcaccttct
      661 tcttcctcct cgaccgagta gccgccctcc cccttcggcc ggtaggtgag gtgttcggct
      721 tcgaagtcga cttcgatccc gtcggccgag gttacgaccg gcgcctcgac ttggtcaggc
      781 acgaagcgga cgatcgactc cttcagccgc tccaggccgg cgccggtcgc agatgaaacg
      841 ggaatgatcc ccagcacgga ttcgccgagc tcgcgatccc attcctcgac ctggtccgcg
      901 atgtcggcct cggtccagag gtcggacttc gaaagcacca gcagctcggg cagcttctcc
      961 agaccggtgc tgtgagcggc aagctcggcc cgtaccgccc ggtagttctc cagcggctca
     1021 ccgtcggagg gggcaagctc gacgacgtgg atcagcatcg cgcagcgttc gacgtgggcg
     1081 aggaactcgt gaccgagccc ggccccctcg gcggcaccct cgatcagtcc cgggatgtcg
     1141 gcgatcacga cctggcggtc cggggtttcg atcgttccga gaaccggctc gagcgtggtg
     1201 aacgggtagt cggcgatctt ggggtcggcc ctggtcaggc gggagagcag cgagctcttg
     1261 ccggcgttgg gcaggccgat caggccggcg tcggccagca gcttcagtct cagttc
//
LOCUS       DAOQFI010000675         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_77898_length_1311_cov_2.978503, whole genome shotgun sequence.
ACCESSION   DAOQFI010000675 DAOQFI010000000
VERSION     DAOQFI010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77898_length_1311_cov_2.978503"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLE93_10465"
     CDS             <1..825
                     /locus_tag="PLE93_10465"
                     /inference="COORDINATES: protein motif:HMM:NF037213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNH87515.1"
                     /translation="KPGNPLPVGAGKPFADLTESGIVPVTRLTHIFRQAARSMITTAA
                     HEVNEGRPPHLTPGPDQVQDFHFMERVTPERVRSAVVEMVGERVEAGLGLDPIRDAQV
                     LAPIYRGEAGIDVLNRELQQRLNPEGRKAVSDRFRIGDRLIQTRNAYEYGLMNGTICF
                     LVDDDPDEEMVVLETDDGQSVELPYAETSDLKLAYAISVHKSQGCEIPVVIFVCHRSH
                     SGMLTRPLIYTAITRAREMCVMVGDRPALENGVRRDEAGRRYSSLAPRLAGTLNHG"
     gene            918..>1311
                     /locus_tag="PLE93_10470"
     CDS             918..>1311
                     /locus_tag="PLE93_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNH87516.1"
                     /translation="MAFAAASFAAMLMLAAPASAQKLVTIDASSDYVDPSNQHLAGPV
                     TENMGPHPGKLMANVLLPDGYTPKKRYPLLLLLHGSGERFDSWADPLLGDIRNTAKGL
                     NAVIVMPEGAQGFYTDWWNEGERSDPAWE"
BASE COUNT          227 a          467 c          408 g          209 t
ORIGIN      
        1 aagcccggca atcccctgcc ggtcggggcc ggcaagccct tcgccgacct gaccgaatcg
       61 ggcatcgtcc cggtcacccg gctgacccac atcttccgtc aggccgcccg ctcgatgatc
      121 accaccgccg cccacgaggt gaacgagggc cgtccgcccc acctcacgcc gggccccgac
      181 caggtccagg acttccattt catggagcgg gtcacacccg aacgggtccg cagcgcggtc
      241 gtcgagatgg tcggcgaacg ggtcgaggcc gggctcggcc tggacccgat ccgcgacgcc
      301 caggtgcttg ccccgatcta ccgcggcgag gcgggcatcg acgtgctcaa ccgcgaactt
      361 cagcagcggc tcaacccgga gggccgcaag gcggtcagcg accgcttccg gattggcgac
      421 cggctgatcc agacccggaa cgcctacgaa tacggcctga tgaacggcac gatctgcttc
      481 ctcgtcgatg acgacccgga tgaggaaatg gtcgtgctcg agaccgatga cggccagtcg
      541 gtcgagctgc cctacgcgga gaccagcgat ctcaagctcg cctacgcgat ctcggtccac
      601 aagtcacagg gctgcgagat cccggtcgtg atcttcgtct gccaccgctc ccactcgggc
      661 atgctcaccc gccccctgat ctacaccgcg atcacccgcg cccgggaaat gtgcgtgatg
      721 gtgggagacc gccccgccct cgaaaacggc gtccgccgcg acgaagccgg ccgccgctac
      781 tcctccctcg ccccccgcct cgccggcacc ctcaaccacg ggtgagctgg ctggttctcg
      841 cgcaagggct taccatgccg cctatgggaa ccatgaagtt ggggagaagt cgttcgttca
      901 gtgctgcccg gaagctggtg gcgtttgccg cggcttcctt tgccgccatg ctgatgctgg
      961 ctgctcctgc ttcggcgcag aagctggtca cgatcgatgc ttccagcgac tatgtggatc
     1021 cctcgaacca gcatctggct ggtccggtga ccgaaaacat gggcccccac cccggcaagc
     1081 tgatggccaa cgtgctgctg ccggacggtt acacgccgaa gaagcgttac ccgctgctgc
     1141 tcctccttca cggttccggc gaaaggttcg actcctgggc agacccgctg ctcggggaca
     1201 tcaggaacac cgcgaagggc ctgaacgcgg tgatcgtcat gccggaaggc gcgcagggtt
     1261 tctacaccga ctggtggaac gaaggcgaac gcagtgaccc ggcctgggag g
//
LOCUS       DAOQFI010000676         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_78356_length_1307_cov_3.149361, whole genome shotgun sequence.
ACCESSION   DAOQFI010000676 DAOQFI010000000
VERSION     DAOQFI010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78356_length_1307_cov_3.149361"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLE93_10475"
     CDS             complement(<1..197)
                     /locus_tag="PLE93_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87517.1"
                     /translation="MRVIIRTALGAAAFVCCLALAGPAAANNFCVPTYSADCPASGTN
                     EAEPDLNAALSAYGSDGNPDRV"
     gene            complement(201..>1307)
                     /locus_tag="PLE93_10480"
     CDS             complement(201..>1307)
                     /locus_tag="PLE93_10480"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNH87518.1"
                     /translation="GGGSFTDIRLFGRNGARFGSAINVACNYPGQVTINGAGVSDSQY
                     GVLAPCSTVPVSIDRATFSGADSALQPSNGAQLTASNVLIESGDLSPVMVYNAKGAGT
                     TTLDLDHATVVATGDASMPAIRANVANVVAATDPIQVNVSNSIILGFDDTWEMIAPTD
                     PAKGDVGLSVSYSDFSPVGVVTGDSSLDTATGNIFDSPGFTGITDYHLTPGAPTVDAG
                     DPSAAGPLFDLEGNGRPIDGDFDSVAVPDMGAYELNPPPPNCPVDPSACAPKLRKVKF
                     RYRFGKGGTLRYRVSIKAKVIAVFRPVPKKAKSKRMTVRILKQGKQGANVIKLGKRLL
                     KPGRYRLAIRALNKTGDSSNVVVRKVQVRKAGRR"
BASE COUNT          203 a          413 c          459 g          232 t
ORIGIN      
        1 accctgtcgg ggttgccatc cgacccgtag gccgagagtg ccgcgttcag gtccggttcg
       61 gcttcgttgg tcccgctggc cgggcaatcc gccgagtagg tggggacgca gaagttgttg
      121 gccgccgccg gcccggccag ggccaggcaa cagacaaacg ccgccgcccc gagggcggtc
      181 cgaatgatca cgcgcatcgt ctaccgcctc ccggctttcc tgacctgcac cttgcgaacc
      241 accacgttgg atgagtcgcc cgtcttgttg agagcgcgga tcgcgagccg gtagcggccc
      301 ggcttcagca ggcgtttgcc gagcttgatc acgttggcgc cctgctttcc ctgcttgagg
      361 atgcggaccg tcatacgctt cgactttgcc ttcttcggga ccggcctgaa gacggcgatc
      421 accttcgcct tgatcgagac ccggtaccgc agggtcccgc ccttgccgaa ccggtagcgg
      481 aacttgacct tccggagttt cggggcgcag gcggacgggt cgaccgggca gttcggggga
      541 ggcgggttga gttcgtaggc gcccatgtcc gggaccgcga cgctgtcaaa gtcaccgtcg
      601 atcgggcggc cgttcccttc gaggtcgaag agcggcccgg cggcggaagg atccccggcg
      661 tcaacggtcg gcgcgccggg ggtcaggtgg tagtcggtga tcccggtgaa cccggggctg
      721 tcgaagatgt tgccggtcgc ggtgtcaagg cttgaatcgc cggtcaccac gccgaccggg
      781 gaaaagtcgg agtaactgac cgaaaggccg acgtcgcctt tggccggatc ggtcggggcg
      841 atcatctccc aggtgtcgtc gaagccgaga attatcgagt tgctcacgtt gacctgaatc
      901 gggtccgtgg cggcaacgac attggccacg ttggcccgga tcgccggcat cgacgcatcc
      961 cccgttgcga ccacggttgc gtgatcgagg tcgagcgtag tcgtgccggc gcccttggcg
     1021 ttgtagacca tgaccgggga gaggtcgcca ctttcgatca gcacgttcga ggcggtgagc
     1081 tgcgcaccgt tggacggctg cagcgccgaa tccgccccgg agaaggtggc ccggtcgatc
     1141 gaaacgggga ccgtggagca gggagccagg acgccgtact ggctgtcgga gaccccggcg
     1201 ccgttgatcg tgacctgccc cggatagttg caggcgacat tgatcgcgct gccgaaccgg
     1261 gcgccgtttc tcccgaatag ccggatgtcg gtgaagctgc ccccgcc
//
LOCUS       DAOQFI010000677         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_78577_length_1305_cov_3.012800, whole genome shotgun sequence.
ACCESSION   DAOQFI010000677 DAOQFI010000000
VERSION     DAOQFI010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78577_length_1305_cov_3.012800"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLE93_10485"
     CDS             complement(<1..297)
                     /locus_tag="PLE93_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNH87519.1"
                     /translation="MDFANNPAGISVSVVTVSDRCFSGEREDGSGPAAVSTLTAAGFA
                     TEGPTVVPDGEESVGSAVKKEIESGADVIVTLGGTGAGERDLTPEGTRPLIEREL"
     gene            440..1231
                     /locus_tag="PLE93_10490"
     CDS             440..1231
                     /locus_tag="PLE93_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH87520.1"
                     /translation="MRIRRLGWAGIEVESGGETVVVDHVLGWKSIESYIGPPRTPLLE
                     PESAGRSLAAFVTHLHTDHTDAAAVSQTLAEGGSLHRPAVMEGEPLETGGVMAAELEL
                     KEAAVDQVLMDEWESVEVGPFVATAVPAVDGFGDPQISWVIEADGKRIIHAGDTLFHG
                     SWWLIQMRFGPFDAAFLPVNGAITSFPHRQPASGLEASLSPEQAVAAARILGAKRLVP
                     IHYDGIDEPPVYQQTDDIISRLQNTLDGEESPELVVMAEGETLDL"
BASE COUNT          224 a          441 c          416 g          224 t
ORIGIN      
        1 cagttcgcgt tcgatcagcg gacgggtccc ttcgggcgtc aggtcccgtt cacccgctcc
       61 ggtgccgccg agcgtgacga tcacgtcggc ccccgactcg atctccttct tcaccgctga
      121 acccacgctt tcttccccgt cggggacaac ggtggggcct tcggtcgcga agccggccgc
      181 ggtcaaggtg ctgaccgccg cggggccgga gccatcctcc ctctctccgc tgaagcagcg
      241 gtcggagacc gtcaccacac tcacggaaat cccggccgga ttgttcgcga agtccatgaa
      301 accagcatat ggagcctgcc tcttcgccca cctcgccccc ggtcgcttga agtacgaacg
      361 atcgtatgct atctattgga tgtcaggtcg ccgggatcga gccggccgcc gcgatcgtca
      421 aagcgatgaa gggaagaaga tgcgaatcag aagacttggc tgggccggga tcgaggtcga
      481 atccggcggg gagaccgtgg tggtcgacca tgtcctcggc tggaaatcga tcgagtccta
      541 catcggcccg ccccgcaccc cgttgctgga gcccgaatcg gctggccgtt ctctggctgc
      601 cttcgtgacg cacctgcaca cggaccacac cgatgccgcc gccgtctcac agaccctggc
      661 cgaaggcggc tcgcttcacc gtccggcagt gatggagggt gagccgctgg agaccggcgg
      721 tgtgatggca gccgagctgg aactgaagga agcggcagtt gaccaggtcc tgatggatga
      781 atgggaatcg gtcgaggtag ggccgttcgt ggccaccgcc gtgccggcgg tcgacggttt
      841 cggcgacccc cagatctcct gggttatcga ggccgacggc aagcgaatca tccatgccgg
      901 cgacacgctc tttcacggtt cctggtggct gatccagatg cgtttcggac ccttcgatgc
      961 cgccttcctc ccggtcaacg gggcgatcac ctcctttccc caccggcagc cggccagcgg
     1021 cctggaagcc tcgctcagcc cggagcaggc ggtcgcggcg gcccggatcc tcggcgcgaa
     1081 gcggctggtt ccgatccact acgacggtat cgacgagccc cccgtctatc agcagaccga
     1141 cgacatcatc tcccggctcc agaacacgct cgacggcgag gaaagcccgg aactggtcgt
     1201 gatggcggaa ggcgagacgc ttgacctctg accggcggca gttcctatag tcaggccatg
     1261 agtccgcgcc ggtcgaaagc cgaagccgct tccacgcatg aggcg
//
LOCUS       DAOQFI010000678         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_79053_length_1301_cov_3.231942, whole genome shotgun sequence.
ACCESSION   DAOQFI010000678 DAOQFI010000000
VERSION     DAOQFI010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79053_length_1301_cov_3.231942"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLE93_10495"
     CDS             <1..607
                     /locus_tag="PLE93_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87521.1"
                     /translation="TPEAGKVAFAVCFAIAISLGLQYGVPLEVFVSKFSYMRFDPEGI
                     TGNPDIPFAKSMPDYIMRWLASQFIDDPDFLEEQGIMTQEVRNRKEAENAGINLANGS
                     GNGSPAGNGGNGNGNGGNGNGGSAPSASTESAAPVADTGGSAVPAGTALTDDVPVVPA
                     RLHFTPGAAELGPACDQCGGMMQRTGACYTCSSCGNNTGCG"
     gene            837..1070
                     /locus_tag="PLE93_10500"
     CDS             837..1070
                     /locus_tag="PLE93_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87522.1"
                     /translation="MGDLVRRKWHLMLIGLIAGAVIVGLVIGPLVVKVFGTVAGVYLI
                     LFLILRRRKGPDGKINPWYASNGRQKGGGIFPF"
BASE COUNT          242 a          408 c          415 g          236 t
ORIGIN      
        1 cacgccggaa gcaggcaagg tcgccttcgc ggtctgcttc gcgatcgcga tctcgctcgg
       61 cctccagtac ggggtgccgc tcgaggtatt cgtgagcaag ttcagctaca tgcggttcga
      121 cccggaaggg atcaccggca acccggacat ccccttcgcc aagtcgatgc cggactacat
      181 catgcgctgg ctcgcctccc agttcatcga cgatcccgac ttcctcgagg agcaggggat
      241 catgacccag gaggtccgca accgcaagga agccgagaac gccggcatca acctggccaa
      301 cggctccggc aacggcagcc cggccggcaa cggtggcaac gggaatggca acggcggcaa
      361 cggcaacggt ggctcggccc cgtccgcatc aaccgagtcg gcggccccgg tcgccgatac
      421 cggcggctcc gcggtcccgg ccggcacggc gctcaccgat gacgtcccgg tggtcccggc
      481 caggctccac ttcactcccg gggcagcaga gctgggcccg gcctgtgacc agtgcggagg
      541 catgatgcag cgaaccggcg cctgctacac ctgctcgtcc tgcggcaaca acaccggatg
      601 tggatgaacc acccaggttg agttcaactc gtttcgaaag ggccccggag agatccgggg
      661 cctttttcct gggcagaccc ggattcatat gcaatacgcg tggctttccg tcctcgccag
      721 gtcagtcgcc cggatcccat tttgaggtgg gtatgaaaag tgtctatatg aacgaatttc
      781 atacccacct cggccacttg gccccggttg ccgggatccg ggattaagat cgcgatatgg
      841 gagatctggt ccggaggaag tggcacctga tgttgatcgg cttgattgcg ggggcggtga
      901 tcgtcggact ggtaatcggg ccgctcgtgg tcaaggtgtt cggcaccgtc gccggcgtct
      961 acctgatcct ctttctgatt ctcaggcgcc gcaagggacc cgacgggaag atcaatccct
     1021 ggtacgccag caacggccgc cagaaaggcg gcggcatctt tcctttctga ggtcggatgt
     1081 caggtggctt tcgcagtgaa ttcaggccgt cgacttgaca aatggtgtga gtgagtgcat
     1141 actcacacct gatggcaacg acttcggaca agatcgcagc gtccccgggg accgggacgg
     1201 ccaggacccg ctcgacggga tcccgctcgc tttcgaccgc tgaagagcgc cgtgaggaac
     1261 tgattacggc ggcgattccg gtctttgccg agaagggtta c
//
LOCUS       DAOQFI010000679         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_79922_length_1294_cov_2.713479, whole genome shotgun sequence.
ACCESSION   DAOQFI010000679 DAOQFI010000000
VERSION     DAOQFI010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79922_length_1294_cov_2.713479"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLE93_10505"
     CDS             complement(<1..708)
                     /locus_tag="PLE93_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87523.1"
                     /translation="MDSTGREQLKRHLPEIILGLLMLAQASFLLVLTAPIAFVSDEWQ
                     FLFFRQGFSPGTTLDPYYEHLVAVPNYLYSTAVGIFGLGTNRPMQIAALLTFLGLNAL
                     LFVYLRRRAGGWAALIGTALILFLGAAFEDLLFAFQINYFGAIACGLGALLALDRDDR
                     RGDLIASLLLVAGVLTSSMIFPFIAAAALEWALNPRDRRRRLFVPGAAIGIWILWWIG
                     WGHQADSQAGLSGLSGLP"
     gene            complement(715..>1294)
                     /locus_tag="PLE93_10510"
     CDS             complement(715..>1294)
                     /locus_tag="PLE93_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNH87524.1"
                     /translation="LAHIRNGRLAPRLFLWMAPSSFLGAVAGGVLAGVLPERILLGAI
                     SLVVLYGAVEVWRYRRPKGDGEGNRPDRRRLLTEAVLIGFGVGLLGGFVGLILGSLRL
                     PAMLKYMGMSPQKAVGTNSAVGVIVGIGGLLGHLPSGVDWNILLIGAAAAVPGAAFGS
                     KLTGKLPEATLVRAMAFVLLVTGLAMAGQAII"
BASE COUNT          250 a          444 c          434 g          166 t
ORIGIN      
        1 gggcagacca ctcagtccgg agagcccggc ctgcgagtcg gcctgatggc cccatccgat
       61 ccaccacagg atccagatgc cgatcgccgc gcccggaacg aagagccgcc ggcgccggtc
      121 cctcgggttg agggcccatt cgagcgcggc ggcggcgatg aacggaaaga tcatgctgga
      181 ggtcagaacc ccggcgacca gcagcagcga ggcgatcaga tcgccccgcc ggtcatcccg
      241 gtccagcgcc aggagggcgc cgagcccgca ggcgatcgct ccgaagtagt tgatctggaa
      301 ggcgaagagc agatcctcga aggcagcccc gagaaagagg atcagggcag tgccgatcag
      361 ggccgcccac ccgcctgccc ggcggcgcag gtagacgaag agcagggcgt tcaggccgag
      421 gaaggtgagc agggccgcga tctgcatcgg ccggttggtg cccagcccga agatcccgac
      481 cgcggtcgag tagaggtagt tcggaaccgc gaccaggtgt tcgtagtagg gatcgagggt
      541 ggtgccgggg ctgaacccct ggcggaagaa gaggaactgc cactcgtcac tgacgaaggc
      601 gatcggtgcg gtcagcacca ggagaaagct cgcctgggcc agcatcagca gaccgagaat
      661 gatctcgggc aggtgtcttt tcagttgctc tctccccgtc gaatccatcg acggtcagat
      721 gatcgcctgc ccggccatcg cgagtcccgt taccagcagg acgaatgcca tcgcccggac
      781 caaagtcgcc tcgggcagct tcccggtcag cttcgaaccg aacgcggcac ccgggaccgc
      841 cgcggccgcg ccgatcagca ggatgttcca gtcaaccccg gaaggaaggt gcccgagcag
      901 gccgccgatc ccgacgatca ctccgaccgc ggagttggtg ccgaccgcct tctgcgggct
      961 catgcccatg tacttgagca tcgccggcag ccgcagcgac cctaggatca gaccgacgaa
     1021 tccgcccagc agcccgaccc cgaaaccgat cagcaccgct tcggtcagga gccggcgccg
     1081 gtcggggcgg ttcccctccc catcgccttt cggccggcgg tatcgccaga cctcgaccgc
     1141 gccgtaaagc acgaccaggc tgatcgcccc gagcaggatc ctttccggca gcaccccggc
     1201 gaggacaccg cccgccaccg cgccgaggaa cgagctcggt gccatccaga ggaagaggcg
     1261 gggggccagc cgcccgtttc tgatgtgggc cagg
//
LOCUS       DAOQFI010000680         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_80171_length_1292_cov_2.683913, whole genome shotgun sequence.
ACCESSION   DAOQFI010000680 DAOQFI010000000
VERSION     DAOQFI010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80171_length_1292_cov_2.683913"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="menB"
                     /locus_tag="PLE93_10515"
     CDS             complement(<1..793)
                     /gene="menB"
                     /locus_tag="PLE93_10515"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNH87525.1"
                     /translation="MEWRSAGEYSDILYEKAEGIAKITINRPEVRNAFRPQCLAELRD
                     AFEKARDDTEVGAIIFTGAGKEAFCSGGDQRIRGDDGYIGDDEVAQQGVGRLDVGDLH
                     VQIRRTPKPVIAMVAGYAIGGGHILHLVCDLTIAGDNARFGQTGPRVGSFDGGFGASL
                     LARNIGVKRAKEVWFLCRHYTAEEALDMGLVNAVVPVDDLEKETVAWCREMLHLSPLS
                     LRLLKSSFNAEEDGLTGLQQLSHDATLLFYMSEEGQEGRNAFQEGR"
     gene            927..>1292
                     /locus_tag="PLE93_10520"
     CDS             927..>1292
                     /locus_tag="PLE93_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNH87526.1"
                     /translation="MKLDNWLAQRSQSCPDRIALIADGESMTYAQLEEEAVHAARRLA
                     AWGVRRGATVAMNMRPGRDKIILVHALMKTGAVLLPISPKLSPEERAKAIESCDVSVD
                     LDDPGKLTQTEADMPLLGEH"
BASE COUNT          221 a          415 c          432 g          224 t
ORIGIN      
        1 tgcgtccctc ctggaaggcg tttcggccct cctggccctc ttcggacatg tagaagagca
       61 gcgtcgcgtc atgggagagc tgctggaggc cggtcaggcc gtcctcttcg gcgttgaagc
      121 tggacttgag aagccgcagg gagagggggg agaggtggag catctcccgg caccaggcca
      181 cggtttcctt ctcgaggtca tcgaccggca cgaccgcgtt caccagtccc atgtcgaggg
      241 cctcttcggc ggtgtagtgg cggcagagga accagacttc cttggcccgc ttgaccccga
      301 tgttccgggc cagcaggctg gcgccgaaac cgccgtcgaa ggacccgacc ctgggcccgg
      361 tctggccgaa acgggcgttg tcgccggcga tcgtcaggtc gcagaccaga tgaaggatgt
      421 gaccgccgcc gatcgcgtat ccggcgacca tcgcgatgac cggtttcggg gtgcgccgga
      481 tctggacgtg gaggtcaccg acatcgagcc ggccgacgcc ttgctgggca acttcgtcat
      541 cgccgatgta gccgtcatcg ccgcgaatgc gctggtcccc gccggaacag aaggcttcct
      601 tgccggcccc ggtgaagatg atcgccccga cttcggtgtc gtcccgggct ttctcgaagg
      661 cgtcacgcag ctccgcgagg cactggggcc ggaacgcgtt ccggacctcg ggccggttga
      721 tggtgatctt cgcgatacct tcggccttct cgtagaggat gtcggagtat tctccggccg
      781 accgccattc gatcggctcg taaagcgtct cttccggggg tgcgttgcgg tgtctttcgg
      841 gcaagtccgg tactccgttc gtcaggccgg agccaggatg gctcgcggca agcaagattt
      901 gagagcacca cgataaacga ggtcaattga aactcgacaa ctggctggcc cagagatcgc
      961 agagctgccc cgaccggatc gccctgatcg cggacggcga gtcaatgacc tacgcccagc
     1021 tcgaagagga agcggtccat gcggcccggc gccttgccgc ctggggagtg cgccgcgggg
     1081 cgaccgttgc gatgaacatg cggccgggcc gggacaagat catcctcgtc catgccctga
     1141 tgaagaccgg cgccgtgctg ctgccgatca gcccgaagct ttcaccggag gaacgggcaa
     1201 aggcaatcga gtcctgtgac gtctccgtcg acctcgacga ccccgggaag ctcacccaga
     1261 ccgaagccga catgccgctg ctcggcgagc ac
//
LOCUS       DAOQFI010000681         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_80252_length_1291_cov_4.172330, whole genome shotgun sequence.
ACCESSION   DAOQFI010000681 DAOQFI010000000
VERSION     DAOQFI010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80252_length_1291_cov_4.172330"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1291
                     /locus_tag="PLE93_10525"
     CDS             <1..>1291
                     /locus_tag="PLE93_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNH87527.1"
                     /translation="VISLVFWSITMIVSVKFVTFIMRADNDGEGGIMALIALIQGASL
                     GNRFVKAGLIGAGLFGVALFYGDGMITPAISVMSAVEGVEVAAPSLSDYVLPMTIGVL
                     VILFAAQRFGTHVIGRAFGPVMVLWFIVIGAAGIGQIAADPEILKALNPAYGVDFFSA
                     HLRIAFISLGAIVLTVTGAEALYADMGHFGRPPIRRAWFLLVFPALTLNYLGQGSLVL
                     QDPSSVSSPFFLLFPDWSHIPMVVLATIATVIASQAVISGAFSVTKQAIQLGYLPRLL
                     IGQIYMPAVNWALFFAVVILVVGFGSSAALASAYGIAVVGTLTIDAILFLVVVRKLWQ
                     KPLWMAICFGVVFLTIDFTFLAANLTKVEHGGWFPLSIGLIILTVILTWDRGYRATAA
                     KRAELEGSLTGLVDEINRKDPGPIRVRGTAVYLNARTN"
BASE COUNT          171 a          434 c          407 g          279 t
ORIGIN      
        1 gtgatctcgc tggtcttctg gtcgatcacg atgatcgtct cggtcaagtt cgtgaccttc
       61 atcatgcggg ccgacaatga cggggagggc ggcatcatgg ccctgatcgc gctgattcag
      121 ggggccagcc tgggcaaccg gttcgtgaag gcggggctga tcggggccgg actcttcggg
      181 gtcgctctct tttacggcga cggcatgatc accccggcga tctcggtcat gtccgcggtc
      241 gagggggtcg aggtcgcggc gccgtctctt tccgattacg tgctgccgat gacgatcggc
      301 gtgctggtga tcctcttcgc agcccagcgt ttcggcaccc atgtgatcgg ccgggccttc
      361 gggccggtga tggtcctctg gttcatcgtg atcggggccg ccgggatcgg ccagatcgct
      421 gccgacccgg aaatcctcaa ggccctcaac ccggcctacg gggtcgactt cttttccgcc
      481 cacctgcgga tcgccttcat ctcgctgggg gcgatcgtgc tcacggtcac cggtgccgag
      541 gccctttacg ccgacatggg ccacttcggc cggcccccga tccgccgggc ctggttcttg
      601 ctcgtctttc ccgccctgac cctgaactac ctcggccagg gatcgctggt cctgcaggac
      661 ccctcctcgg tttccagccc gttctttctg ctcttccccg actggtcgca catcccgatg
      721 gtcgtcctcg ccacgatcgc cacggtgatc gcctcgcagg cggtgatctc cggtgctttc
      781 tcggtgacca agcaggcgat ccagctgggg tacctgccgc gcctcttgat cggccagatc
      841 tacatgccgg ccgtcaactg ggcgctgttc ttcgcggtgg tgattctggt ggtcggtttc
      901 ggctcctcgg ccgcgctggc ctccgcctac gggatcgccg tggtcggcac cctgacgatc
      961 gacgcgatcc tcttcctggt cgtggtcagg aagctctggc agaaaccgct ctggatggcg
     1021 atctgtttcg gggtcgtttt cctgaccatc gacttcacct tcctcgctgc gaacctgacc
     1081 aaggtcgaac atggcggctg gttcccgctc tcgatcggcc tgatcatcct gaccgtgatc
     1141 ctgacctggg atcgcggtta ccgggcgacc gcggcaaagc gggccgagct ggaaggttcg
     1201 ctcaccggac tggtggacga gatcaaccgc aaggatccgg ggccgatccg ggtccggggc
     1261 accgccgtct acctgaacgc ccggaccaac a
//
LOCUS       DAOQFI010000682         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_80263_length_1291_cov_3.435275, whole genome shotgun sequence.
ACCESSION   DAOQFI010000682 DAOQFI010000000
VERSION     DAOQFI010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80263_length_1291_cov_3.435275"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLE93_10530"
     CDS             <1..583
                     /locus_tag="PLE93_10530"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH87528.1"
                     /translation="KSLRNGSASVVTDRIDTFTESGIRLESGKELEADIVITATGLNV
                     QLFGGIEVTIDGETFDAPDTVAYKGLMLSDVPNLVFAVGYTNSSWTLKIDLVCEYFCR
                     LLDHMDRTGAEIAVARVTDPEMERIPLLDFPAGYVLRALDSLPKQGTKEPWYLAQNYA
                     KDSRFLREGNVADEYMEFRQIERKSTAKEEIPA"
     gene            580..>1291
                     /locus_tag="PLE93_10535"
     CDS             580..>1291
                     /locus_tag="PLE93_10535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH87529.1"
                     /translation="MSEVRGKVCVVTGAGSGIGRALALELARRGARAVAISDIKLETA
                     EATAELIGAGTEIHTAALDVSDREAFAAYASEVAGTFGQVHQIYNNAGVADNDPVTEF
                     DYDRYERILNVNLWGVIYGTKEFLPHLIASGVGHVINVSSLNGIMAQQGLSAYCASKF
                     AVRGFTESVAFDLKGEGLPVRTTVVHPGGIKTSIADSAMEWARSAGLPVTPEQEARKA
                     FYNEKLLKMDPARAARIVI"
BASE COUNT          261 a          399 c          426 g          205 t
ORIGIN      
        1 caagtcactc agaaacggct cggcctcggt cgtgaccgac cgtattgaca ccttcactga
       61 aagcgggatc cggctcgagt cgggcaagga gctcgaggcc gacatagtga tcaccgcgac
      121 cgggctgaac gtccagctct tcggtggcat cgaggtcacg atcgacggcg agaccttcga
      181 tgctccggac acggttgcct acaagggcct gatgctgagc gacgtgccga acctcgtctt
      241 cgcggtcggg tacacgaact cgtcctggac gctgaagatc gacctggtct gcgagtactt
      301 ctgccggctg ctcgatcaca tggaccggac cggagcggaa atcgccgtgg cccgggtcac
      361 ggacccggaa atggagcgga ttccgctgct cgacttcccg gccggctacg tgctgcgggc
      421 tctggacagc ctgccgaagc agggcacgaa ggaaccctgg tacctggccc agaactacgc
      481 gaaggattcc cgattcctcc gggaaggtaa tgtcgcagat gagtacatgg agttccgcca
      541 gatcgagcgg aagtcaacgg cgaaggagga gatccccgct tgagtgaagt cagaggaaag
      601 gtctgcgtag taactggcgc ggggtccggc atcggtcggg ccctcgcact tgagctggca
      661 aggcggggag cccgggcggt cgcgatcagc gacatcaagc tcgaaaccgc cgaggcaacc
      721 gcggagttga ttggtgccgg gacggagatc cacaccgcag cgctcgacgt gagtgaccgc
      781 gaggcgttcg ccgcctatgc gagcgaagta gccggaactt tcggccaggt ccaccagatc
      841 tacaacaacg ccggcgtcgc cgacaacgac ccggtcaccg agttcgacta cgaccgctac
      901 gagaggatcc tgaacgtgaa tctctggggc gtgatctacg gcaccaagga gttcctgccg
      961 catctgatcg cctccggcgt cggccatgtc atcaacgtct ccagcctcaa cgggatcatg
     1021 gcccagcagg ggctgagcgc ctactgcgcg agcaagttcg cggtccgcgg cttcaccgaa
     1081 tcggtcgcct tcgacctgaa gggcgaaggg cttccggtgc ggaccacagt ggtccatccc
     1141 ggcggcatca agacgagcat cgccgattcg gcgatggagt gggccaggag cgccggactt
     1201 ccggtgacgc ccgagcagga ggcgaggaaa gccttctaca acgagaagct gctgaagatg
     1261 gaccccgccc gcgccgcccg gatcgtgatc g
//
LOCUS       DAOQFI010000683         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_80809_length_1287_cov_1.875000, whole genome shotgun sequence.
ACCESSION   DAOQFI010000683 DAOQFI010000000
VERSION     DAOQFI010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80809_length_1287_cov_1.875000"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..>1287)
                     /locus_tag="PLE93_10540"
     CDS             complement(13..>1287)
                     /locus_tag="PLE93_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87530.1"
                     /translation="PPGFKPFLPDSPGYDWSLLDQAIRLTRANGLTPIVMAFEAPKWA
                     EGPNRDPNLDERSHPGVWKPNPDQFAAFGRALASRYNGRHPDPVNPGSSLPKVRYFQA
                     WNEPNLNYYLAPQAEKGRIVSPNLYRPLLNRFYTSVKSVSPDAQVLAAGFAPLGSMKN
                     VAVAPLEFLRKLTCVTKNHKLRKGCSSFIKADIYDQHLYTSGGPTHKAGGPDEIALGD
                     LPELRRMLKAVDRFNRIKGKFKRTKLFSTEFSWDTAPDPGGLPMGLAKRWVAEALFRM
                     WQSGISVMTWQGIVDQAYPAPGDEWLWKYTIQSGFFYYATEPSKAVKKGSYTAFRFPF
                     VAFKVPRGIRIWGRTPNSKGGKIRIQQNRGSGWRRVLVVRANKFGVFSRTFRSRKKTG
                     RLRALYGASNSVPFSLKRVPDRFVRPFGGLDD"
BASE COUNT          275 a          374 c          362 g          276 t
ORIGIN      
        1 ggagtctgtc gctcaatcgt cgagcccgcc gaaaggcctg acaaagcggt caggcactcg
       61 cttgagtgag aaggggacgg agttggaagc tccataaagc gctcgcaggc gaccagtctt
      121 cttcctgctc cggaacgttc ggctgaacac cccgaacttg ttggctctaa ccaccagcac
      181 ccgccgccaa cccgaacccc tgttctgctg gatgcggatc ttgccgccct tgctgttggg
      241 agtcctgccc caaattctga tgccccttgg caccttgaag gcgacgaatg ggaaacgaaa
      301 tgctgtatac gaacccttct tgacggcttt gctgggttcc gtcgcgtagt agaaaaaccc
      361 tgactggatt gtgtatttcc acagccactc gtccccgggg gcgggatagg cctgatcgac
      421 gatgccttgc caggtcatca cggaaatgcc cgactgccac attcgaaaca gagcttcggc
      481 aacccatcgt ttggccaatc ccatcggcaa cccgcctgga tccggcgccg tgtcccacga
      541 gaactcggtc gaaaagagtt tcgtccgttt gaacttgccc ttgatccggt tgaagcgatc
      601 aactgccttg agcatccgcc ggagctctgg caggtcgccc agagcaatct cgtcgggtcc
      661 gccagccttg tgagtaggtc cacccgacgt atatagatgc tgatcgtaaa tatctgcctt
      721 gatgaagctc gaacaaccct ttctcagctt gtgattcttc gtcacacatg tgagcttgcg
      781 aagaaactcc aatggagcga cagccacatt cttcatcgag ccgagtggag cgaaaccggc
      841 cgccaagacc tgcgcatcgg gagaaaccga ctttacggag gtgtagaacc tgttgaggag
      901 tggccggtag aggttgggag agacgattct ccccttttca gcctgcggcg ccaaatagta
      961 attcagattc ggctcgttcc acgcttgaaa gtacctgacc ttggggagac ttgagccggg
     1021 gtttaccgga tcagggtgtc tgccattgta cctcgaggcc agcgccctgc cgaaagcggc
     1081 gaactgatcc gggttcggct tccagactcc tggatgggat ctctcatcaa ggttagggtc
     1141 tcggtttgga ccttcggccc acttcggagc ctcaaatgcc atgacaatcg gggtcagacc
     1201 attcgcacga gttagcctga ttgcctggtc aagaagagac cagtcatacc ccggcgagtc
     1261 aggcaggaac ggtttgaagc caggcgg
//
LOCUS       DAOQFI010000684         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_81017_length_1285_cov_2.418699, whole genome shotgun sequence.
ACCESSION   DAOQFI010000684 DAOQFI010000000
VERSION     DAOQFI010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81017_length_1285_cov_2.418699"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..>1285)
                     /locus_tag="PLE93_10545"
     CDS             complement(50..>1285)
                     /locus_tag="PLE93_10545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNH87531.1"
                     /translation="ALVVILVLIWQTSELSPFLYRGGMVILALATTVLVAVLAHPATR
                     LGPIIGCRPLRWIGVRSYAIYLWQLPVIILTTPVGSSGFHPLRAPVQVATIFLIAALS
                     WKYIEDPVRKGAVGRLWKRFRAGEFSWKKFGPEGKGFTVAGGVVVLVLLMAMVGVAPS
                     SQLAPIATTGGAGQAEVEAIATAAPVAKTVISQKDRDEFPKSLCSSVAYIGDSTSLGL
                     YGPEADQYLPDKNDQLPARLDDVGVYEQHIDISGARSSYEEVEGMPNAVEAAQAIVDA
                     GFDGCWIFAMGTNDTANVAVGSAVGMRERIDSLMGVADGNPALWINVRTLNPPNPAYS
                     EEQMQAWDDELLNACEDYPNMRVYDWANKVKDEWFIDDGIHFTSEGYKVRAQAIANAV
                     PKAFGIWSSFWNQSGCLIE"
BASE COUNT          233 a          419 c          418 g          215 t
ORIGIN      
        1 cggccgccga cgaagccagc ggacggctgg tggttcctgg cgccgcccgc tactcgatta
       61 aacagccgga ctgattccag aagctggacc agatgccgaa ggccttcggg accgcgttgg
      121 cgatcgcctg cgcgcgcacc ttgtaaccct cggaggtgaa gtggatgccg tcatcgatga
      181 accactcgtc cttgaccttg ttggcccagt cgtagacgcg catgttcggg tagtcctcgc
      241 aggcgttcag caactcgtcg tcccaggcct gcatctgctc ctccgaatag gccggattcg
      301 gcgggttcag ggtgcggacg ttgatccaga gcgccggatt tccgtcggcc acgcccatca
      361 ggctgtcgat cctctcccgc atcccgaccg ccgagccaac cgccacgttc gcggtgtcat
      421 tggttcccat cgcaaagatc cagcatccgt cgaacccggc gtcgacgatc gcctgggccg
      481 cttcgaccgc gttcggcatg ccttcaactt cctcgtagga ggagcgggcc ccggaaatgt
      541 cgatgtgctg ctcgtagaca ccgacatcgt cgaggcgggc cggcaactga tcgttcttgt
      601 cgggcaggta ctggtccgcc tccggcccgt agaggccaag cgaggttgaa tcgccgatgt
      661 aggcgaccga gctgcagaga ctcttgggga actcgtcgcg gtccttctgg ctgatcacgg
      721 tcttggcgac tggtgccgcc gtggcaatcg cctcgacctc cgcctgtccg gcgccgccgg
      781 tcgtggcgat cggggccagc tggctggaag gcgcaacccc gaccatggcc atcagcagga
      841 ccaggacgac taccccgccg gcgacggtaa atcccttgcc ttccgggccg aacttcttcc
      901 agctgaactc acccgcccgg aagcgcttcc agagccggcc gaccgctccc ttgcggaccg
      961 ggtcctcgat gtacttccag gaaagcgccg cgatcaggaa gatggtcgcg acctgaaccg
     1021 gagcccgaag cggatgaaag ccgctggaac caaccggggt ggtcaggatg atcacgggca
     1081 actgccagag atagatcgcg taggagcgaa ccccgatcca gcgcagcggg cggcaaccga
     1141 tgatcggtcc gagtctggtc gccggatgcg cgaggacggc aaccagaacg gtggtggcaa
     1201 gggcgaggat gaccatgccc ccgcggtaca ggaagggtga gagctcggag gtctgccaga
     1261 tcaggacgag gatgaccacc agggc
//
LOCUS       DAOQFI010000685         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_81021_length_1285_cov_2.312195, whole genome shotgun sequence.
ACCESSION   DAOQFI010000685 DAOQFI010000000
VERSION     DAOQFI010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81021_length_1285_cov_2.312195"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLE93_10550"
     CDS             complement(<1..632)
                     /locus_tag="PLE93_10550"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNH87532.1"
                     /translation="MSTESDPRFRLAASQVLEERATQISVLAKGVLDVTETGAADELW
                     LASRRLRAALEVFRSCFGRAQYREGRAEARRISRAAGARRDIDVVIAAYESVGEETAP
                     AESEGIDRVISSLRREQASANRALAQVVHGRRLQAFRVRIEEIADSAVEADAADRAHD
                     YRPVETLTPEAIALISKRLARLRKEAVDSLDPEGVDAQHRLRVAAERLRYS"
     gene            complement(661..>1285)
                     /locus_tag="PLE93_10555"
     CDS             complement(661..>1285)
                     /locus_tag="PLE93_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87533.1"
                     /translation="EAVAGIPVHVVSENEEGRLAFLGATKALGYQVEGRVAVVDVGGG
                     STEIIIGTADGGVEQVRSWPIGSGQLAEELISSDPPSASEIRRVRDRVDEVFAEVQIT
                     QPEQAVAVGGSATSLRKIVGARLEYETLERSIRVLSGDEALEVGRQFELDPIRVRMLA
                     TGVLILEKVSELLGQDLQIGKGGIREGIVLDMLNGGIDVSEPALTPG"
BASE COUNT          202 a          451 c          419 g          213 t
ORIGIN      
        1 gagtacctga gccgctctgc cgcgacccgc agccggtgtt gcgcgtcgac gccttccgga
       61 tcgaggctgt caaccgcttc cttgcgcagc cgggccagcc gtttggagat cagggcgatg
      121 gcttcgggag tcagggtttc aaccggccgg tagtcatggg cgcggtcggc ggcgtctgct
      181 tcgaccgccg aatcggcgat ttcctcgatc ctgacccgga acgcctgcag gcggcggccg
      241 tggaccacct gggccagggc gcggttggcg ctggcctgtt cacggcgcag cgacgagatc
      301 acacggtcga tcccctccga ttcggccggg gcggtttcct caccgacgct ttcgtaggcg
      361 gcgatcacca cgtcgatgtc gcgcctcgcc ccggcggccc gcgaaatccg cctggcttcc
      421 gcccttccct cgcgatactg ggcccggccg aaacaggagc gaaagacctc aagtgcggcg
      481 cgaagccgac gggaagcgag ccagagttcg tcggccgctc cggtctcggt cacgtcgagc
      541 acacccttgg caaggaccga gatctgggtc gcccgctctt cgagcacctg cgacgcggca
      601 aggcggaagc ggggatcgga ttcggtgctc atcaaccgga accctagacc tcaggccgac
      661 tcagccgggt gtcaatgcgg gttctgaaac gtcgattccg ccgttcagca tgtcgagcac
      721 gatcccttcg cggatcccgc ccttgccgat ctgcaggtcc tggccgagca gttcggagac
      781 cttctcgaga atcaggaccc cggtcgccag catgcggacg cgaatcggat cgagttcgaa
      841 ctggcggccg acttcgagcg cttcgtcacc gctgaggacc cggatgctgc gttccagagt
      901 ctcgtattcg agccgggcgc cgacgatctt gcgcagtgaa gtggccgaac cgccaaccgc
      961 aacggcctgc tcgggctggg tgatctggac ctcggcgaag acttcgtcga cccggtcccg
     1021 cacccggcgg atctcggaag ccgagggcgg gtccgagctg atcaattcct cggccagctg
     1081 gcccgagccg atcggccagg agcggacctg ttccaccccg ccgtcggccg tgccgatgat
     1141 gatctcggtc gaaccgccac cgacgtcgac cacggccacc cggccttcga cctgatagcc
     1201 gagcgccttg gtcgccccga ggaaggcgag ccggccttcc tcgttctcgc tgaccacgtg
     1261 aaccggaatg cccgccaccg cctcg
//
LOCUS       DAOQFI010000686         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_81118_length_1284_cov_2.713588, whole genome shotgun sequence.
ACCESSION   DAOQFI010000686 DAOQFI010000000
VERSION     DAOQFI010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81118_length_1284_cov_2.713588"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..692)
                     /locus_tag="PLE93_10560"
     CDS             complement(90..692)
                     /locus_tag="PLE93_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87534.1"
                     /translation="MTEGNGKFDPEQLGGLDDPVRRYLTRALAPGMTVPDAWSLRMKG
                     SIKVGLRLPFEAGQELDGRSFRWRARVPSNRFPLLEVLDYFDGTGGGMTGRGFGRQLF
                     ADTSADAARSAATRAVMESTMVPATLRPGTGIEWTARSETEIRFRRPAVSLAEEVISG
                     SIPTDARPRSKHRAGARKRTSRAGSSRFSAASTENGNSPE"
     gene            complement(727..>1284)
                     /locus_tag="PLE93_10565"
     CDS             complement(727..>1284)
                     /locus_tag="PLE93_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87535.1"
                     /translation="STVKVMLMTSYLRSRPVRRRNLHPGERRLIRRMIRHSDDFAATE
                     IRNRIGHSGLPHLARTVGIHAFIYSAIWGECRSNPRSQAGFMRRLPDLLPRRHRRFAL
                     RQLNRITPSQRWGIGRVRPHGWRLYFKGGWGSGSGAVDHQIALLRRGRHRIGLSIQTM
                     NNPSHAYGKVTLRGVAARLLRGLPG"
BASE COUNT          208 a          437 c          428 g          211 t
ORIGIN      
        1 ggagatggcc tcgatcgagg tgatccgggc cgagaagaac ggcttgaact caccttcgac
       61 ccagccggcg gtcatcctgg ccggcacctt cattccggcg aattcccgtt ctccgtcgaa
      121 gcggcagaga aacgggagga gcccgcccga ctggtccttt tccttgcgcc agcgcggtgc
      181 ttcgacctcg gtcgcgcgtc cgtcgggatc gatccggaga tcacctcctc ggcgagcgaa
      241 accgcggggc gccggaagcg gatctcggtt tccgagcgcg cggtccattc gatcccggtg
      301 cccggccgca gcgtggccgg caccatcgtc gattccatca cggccctggt tgcggccgag
      361 cgggcggcgt ccgcgctcgt atcggcgaag agttgccggc cgaacccgcg gccggtcatt
      421 ccgccgcccg tcccgtcgaa gtaatcgagc acctcgagga gcgggaaccg gttcgaaggt
      481 acccgggccc gccacctgaa cgacctgccg tcgagttcct gaccggcctc gaagggcaac
      541 cgcagaccga ccttgatcga acccttcatc cggaggctcc aggcatctgg aacggtcatg
      601 ccgggcgcca gggcgcgagt caggtatcgg cggaccggat catcgagtcc gcccagttgt
      661 tccggatcaa atttgccgtt tccctcggtc atcgcctcac ctgaccggaa ggttacgggc
      721 cgcgagtcag cccggcaggc cgcgcagcaa ccgggcagcc actcctcgca gggtcacctt
      781 gccgtatgcg tgggacgggt tgttcatggt ctggatcgag agtccgatcc ggtgcctgcc
      841 gcggcggagc agggcaatct gatggtcgac cgcgccgctg ccgctgcccc agccgccctt
      901 gaagtagagc cgccatccgt gcggcctcac ccgaccgatt ccccagcgct gggacggagt
      961 gatccggttg agctgccgca gggcaaaccg gcggtgacgc cgcggcaaaa ggtccggaag
     1021 gcgcctcatg aaaccggcct ggctgcgcgg gtttgaccgg cactcgcccc atatcgccga
     1081 gtagatgaaa gcgtggatgc cgaccgtccg ggcgaggtgc ggcagtcccg agtgaccgat
     1141 ccggttgcgg atctcggtcg ccgcgaagtc atcggagtgc cggatcatcc gcctgatcag
     1201 ccgacgctca ccggggtgaa ggttccggcg gcgaaccggc cggcttctca ggtatgaggt
     1261 catcagcatc accttgaccg tgct
//
LOCUS       DAOQFI010000687         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_81378_length_1282_cov_2.645477, whole genome shotgun sequence.
ACCESSION   DAOQFI010000687 DAOQFI010000000
VERSION     DAOQFI010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81378_length_1282_cov_2.645477"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..>1282)
                     /locus_tag="PLE93_10570"
     CDS             complement(3..>1282)
                     /locus_tag="PLE93_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNH87536.1"
                     /translation="SAAIERTGFSQVGVVGMGYVGLPLAVAFAEAGCQVIGLDADPTK
                     VASLNRSTTYIEDVPSSLLESIGDGIRATVDFDELAECDAIVICVPTPLTGSSEPDLT
                     YLESAGENLAEILAPGQLVVLESTTYPGTTRDLLAGKLEEGGLKAGEDFHLAFSPERI
                     DPGRTDYTVKTTPKLVGGISPECTDRAATLYETVCDQVVRLSGPEAAELSKLLENIFR
                     SVNIALVNELAQLCDRLDVDVWEVVEAASTKPFGFMPFEPGPGMGGHCLPIDPFYLAF
                     TARQNDFYPEFIELAGKINQSQPRFCVRRIERILNNQMRSVKDSKILILGVSYKAGVG
                     DTRESPAIKIIHLLAELGADLRYHDPYVPELKDAGLEHSEDLDAALEEVDLAVIVTSH
                     PEVDLDTVVDKSKSLLDLRGVTRKHEGAHITRL"
BASE COUNT          239 a          365 c          407 g          271 t
ORIGIN      
        1 ggctatagcc gcgtgatgtg ggcgccttcg tgcttccggg tgacgccacg caggtcgagt
       61 agcgacttgc tcttgtcgac gacggtgtcg agatcgacct cggggtggga ggtgactatg
      121 actgccaggt cgacctcttc gagagcagca tcgaggtctt ccgaatgctc caacccggcg
      181 tccttcaact ccggcacata cgggtcgtga tagcggaggt cggctccgag ctccgccagc
      241 agatggatga tcttgattgc cggcgattcc cgggtgtctc cgaccccggc cttgtaactg
      301 actccgagga tcaggatctt cgagtccttg actgatcgca tctgattatt gagaattcgc
      361 tcaatccgtc tgacgcagaa gcgcggctgc gactggttga tcttgccagc aagttcgatg
      421 aactcggggt aaaaatcgtt ctgccgggcg gtgaaggcga ggtagaatgg gtcgatcggc
      481 aggcagtgtc ctcccatgcc ggggccgggc tcgaacggca tgaacccgaa tggcttggtt
      541 gaggccgctt ccaccacctc ccagacatcg acgtcaagcc ggtcgcaaag ctgggccagt
      601 tcgttaacga gggcgatgtt gaccgaacgg aaaatgttct cgagcagctt cgaaagctcg
      661 gcggcttccg gccccgacag ccgaacaacc tggtcgcaca ccgtttcgta aagggttgcg
      721 gcgcggtctg tgcattctgg cgagattcct ccgaccagtt tcggtgtggt cttgaccgtg
      781 tagtcggtac gacctggatc aatccgctcc ggggagaagg ctaggtggaa gtcctctccg
      841 gccttcagtc ctccctcctc cagtttcccg gcaagaagat ccctggtggt gccggggtaa
      901 gtcgtcgatt cgagaacgac gagttgaccg ggagcaagga tctcagcgag gttctcaccg
      961 gcagactcca gatatgtgag gtctggttcg cttgacccgg tgagtggggt cgggacgcag
     1021 atgacgatcg cgtcgcattc ggccagttcg tcgaagtcga cggtggcgcg aatgccatcg
     1081 ccgatgctct cgagcaagga cgacggtacg tcctcgatgt atgtagtcga gcggttcagg
     1141 cttgcgacct tggtcggatc ggcatccagg ccgatcacct gacatccggc ttcggcgaag
     1201 gcgactgcca gtggcagccc gacatatccc attccgacga cacctacctg gctgaagccg
     1261 gttctttcta tagcggcaga gg
//
LOCUS       DAOQFI010000688         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_82108_length_1276_cov_4.563473, whole genome shotgun sequence.
ACCESSION   DAOQFI010000688 DAOQFI010000000
VERSION     DAOQFI010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82108_length_1276_cov_4.563473"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLE93_10575"
     CDS             complement(<1..610)
                     /locus_tag="PLE93_10575"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH87537.1"
                     /translation="MTNARIAEALEELAVLYQLDGADQYRVLAYTNSARTIKNEGRTV
                     EEMARNGTITELPGIGKTLEEKIVALVETGEIPAAAKLKERIPVGLLEISRIPGLGPK
                     TVRRLHDELDVNGPEDLRAAAEAQKVRELKGLGPKVEEKILAGLDRLDAEPEGPTRLR
                     LDEILPTAEELAAALRAESSCDRAEIAGSVRRLAETCHDIDLI"
     gene            654..>1276
                     /locus_tag="PLE93_10580"
     CDS             654..>1276
                     /locus_tag="PLE93_10580"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNH87538.1"
                     /translation="MPDEARTEHRGAAWVILPTYNEAGNVEKMVDALGPKLTHDDDRI
                     LIVDDNSPDGTGQIADRIATENPRVRVLHRKVKEGLGPAYIEGYRVALEGGADLIVQM
                     DADFSHDPAYLPRLLAASELADLVIGSRYVPGGGITEWGQVRRLLSRGGSLYSRTVLR
                     VPVRDMTGGFKCFRRKVLETIDLSQVAASGYSFQVEMTWRVVQAGFSV"
BASE COUNT          190 a          426 c          422 g          238 t
ORIGIN      
        1 cgatcaggtc gatgtcatgg caggtctcgg caagccggcg gacggagccc gcgatctcgg
       61 ccctgtcaca gctcgactcc gctctgagcg cggcggcgag ctcctcggcg gtcgggagga
      121 tctcatcgag acgcagccgg gtcgggcctt ccggttcggc gtcgagccgg tcgaggccgg
      181 cgaggatctt ctcttcgacc ttcggtccca gccccttgag ttcacggacc ttctgcgcct
      241 cggcggcggc gcgcaggtct tcgggcccgt tgacatcgag ttcgtcatgc aggcggcgga
      301 cggtcttcgg gccgagcccg gggatcctgc tgatctcgag caggcccacc gggatcctct
      361 ccttcagctt cgccgcggcc gggatctcgc cggtttcgac cagggcgacg atcttctctt
      421 caagcgtctt gccgatcccg ggcagttcgg tgatcgtgcc gttccgggcc atctcctcga
      481 ccgtacggcc ctcgttcttg atcgtccggg ccgagttcgt gtaggcgagg acccggtact
      541 gatcggcccc gtccagctgg tagagaacgg ccagctcttc aagcgcttcg gcgatccggg
      601 cgttggtcat acccagaaac tacctccaac cctttgaggg gtacgatccg ccggtgccgg
      661 atgaagccag gacagagcac cgcggcgcgg cctgggtcat cctgccgacc tacaacgagg
      721 ccggcaacgt cgagaagatg gtcgatgcac tcggtccgaa gctgacccat gacgacgacc
      781 ggatcctgat cgtcgacgac aattccccgg acggtaccgg gcagatcgcc gatcggatcg
      841 cgaccgaaaa tccccgggtc cgggtgcttc accgcaaggt caaggaaggt ctcggacccg
      901 cttacatcga ggggtaccgg gttgcgctcg agggcggggc cgacctgatc gtccagatgg
      961 acgccgactt ctcccacgac ccggcctacc tgccgcggct tctggccgcc tcggagcttg
     1021 ccgacctggt gatcggctcg cgctacgtgc ccggcggggg gatcaccgaa tggggccagg
     1081 tccggcgact gctcagccgt ggcggcagcc tttactcgag gacggttctg cgggttccgg
     1141 tccgggacat gacgggtggc ttcaagtgct tccggcggaa agtcctcgaa acgatcgacc
     1201 tctcccaggt tgccgcctcc ggttattcct tccaggtcga aatgacctgg cgggtcgtcc
     1261 aggccggttt ctcggt
//
LOCUS       DAOQFI010000689         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_82118_length_1276_cov_3.448812, whole genome shotgun sequence.
ACCESSION   DAOQFI010000689 DAOQFI010000000
VERSION     DAOQFI010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82118_length_1276_cov_3.448812"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1276)
                     /locus_tag="PLE93_10585"
     CDS             complement(<1..>1276)
                     /locus_tag="PLE93_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNH87539.1"
                     /translation="LGPYQIRFSDSPFGFSISRGGRYLLAGLGHSFERNGVRYATLGY
                     TPQPAKELEPPDLSAAPDEEPSTQTSYVATRANGMWFGPDVYEARPRTDSPNGRRMRL
                     TIWLRKDGTLGIRAGMMAGKAGSVYLGFRSPQDEAFHGFGGRREGTDLRGSDIKSWVL
                     DYRYPDPTTAYYSPVPGFISSRGYGLLLKGDRISRWRMASDRPTAWRVSRPGGSMEMT
                     LTSGGQARAMAGISRLTGRHRLPPAWATGTVLSRAIGIAADSGGKYQSRVESDLEHLA
                     SADFPISAYAFEGWQALPGSFVKATIRTLKEEGIHPVLYLRSFVSNDTAGTEGPGTFD
                     QAISRKLVATGKDGKPFLLPSPFPGGQAAVIDLTKPAAVRWWRNRIWNLLDLGADGFM
                     NDFGEQVEAGMRFHDGTSAALMHNRYPALQAKV"
BASE COUNT          206 a          430 c          414 g          226 t
ORIGIN      
        1 acttttgcct gcagggccgg gtagcggttg tgcatcagcg cggcactggt cccgtcgtga
       61 aatctcatgc cggcttcgac ctgttcgccg aagtcattca tgaaaccgtc ggcgccgagg
      121 tccaacagat tccagatccg gttccgccac cacctcaccg cggccggttt ggtcaggtca
      181 atcaccgccg cctgaccgcc cgggaagggc gagggcaaaa ggaacggctt cccgtccttg
      241 ccggtcgcaa ccagcttgcg ggaaatcgcc tggtcgaagg tgccggggcc ttccgtgccc
      301 gcggtgtcat tcgaaacgaa cgaacgcaga taaagcaccg ggtggatgcc ttcttccttc
      361 agggtccgga tcgtcgcttt gacgaagctc ccgggcagcg cctgccagcc ttcgaaggcg
      421 taggccgaga tcgggaaatc cgcgctggcc agatgttcaa gatctgactc gacccgcgac
      481 tggtatttgc caccggagtc cgcggcgatc ccgatcgccc gggaaagaac ggtcccggtc
      541 gcccaggcgg gcggcaggcg gtgccttccg gtcagccggg agattccggc catcgcccgg
      601 gcctgaccgc ccgaggtcag ggtcatttcc atcgacccgc cgggccggct gacccgccag
      661 gctgttggcc ggtcgctggc catccgccag cggctgatcc ggtcgccttt cagcagcagg
      721 ccatagccac gcgatgagat gaatccggga accggagagt agtaggcggt ggtcgggtcc
      781 ggatagcggt agtcgagcac ccagctcttg atgtcggaac cacgcaggtc ggttccttcc
      841 cggcggccgc cgaaaccgtg gaaggcctcg tcttgcggcg agcggaaccc gaggtagaca
      901 gagcctgcct tgcccgccat catccccgcc ctgattccca gggttccatc ctttctcagc
      961 cagatcgtca gccgcatccg ccggccgttg ggcgagtcgg tccgtggccg cgcctcgtaa
     1021 acatccgggc cgaaccacat tccatttgcc ctggtcgcca cgtacgaggt ctgcgtggac
     1081 ggctcttcgt cgggcgccgc cgacaggtcc ggcggttcga gctccttcgc cggttgcggg
     1141 gtgtatccca gggttgcgta gcgcaccccg tttcgctcga aactgtggcc cagcccggcc
     1201 agcaggtacc ggccgcccct gcttatgctg aagccgaatg gcgagtcgga gaaccggatc
     1261 tggtacgggc ccaggc
//
LOCUS       DAOQFI010000690         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_82257_length_1275_cov_3.255738, whole genome shotgun sequence.
ACCESSION   DAOQFI010000690 DAOQFI010000000
VERSION     DAOQFI010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82257_length_1275_cov_3.255738"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..290)
                     /locus_tag="PLE93_10590"
     CDS             complement(66..290)
                     /locus_tag="PLE93_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87540.1"
                     /translation="MHIFAYLDPGSVSAALAAVLAGVAGIGAAIRTFGSNLKSKLMFW
                     KKDEPAPEAAATATSAAPTDAPAEDKPAEQ"
     gene            complement(363..>1275)
                     /locus_tag="PLE93_10595"
     CDS             complement(363..>1275)
                     /locus_tag="PLE93_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase"
                     /protein_id="HNH87541.1"
                     /translation="LEMNTRVQVEHCVTEEITGVDIVREQIKIAYGEELSLKQEDVTI
                     DGWAIECRINAEKAHMNFAPAPGPIEVYDEPSGPGVRVDSGVKAGSEVTPMYDPMVAK
                     LIVWDKDREAATRRMLRALDEYEIAPLSTLIPFHKTILRTEQWARGETCKDLTEDKKW
                     LKTTAPEDTSIPEPADGEAEKVARDYLVEVSGKRFDVKVIGEATGFAAAGAAPGAGGP
                     KPKREKRAGGGGGGGGPVLASPLQGSIFKLEVAEGAEVAEGDLICVIEAMKMENEITA
                     HKAGKVTKVSISVGDAVASGDPLVTIE"
BASE COUNT          183 a          421 c          415 g          256 t
ORIGIN      
        1 catctgaaac gacgacgcgg ctgtcccggt ccctgaacga gcccggttct gtggccgcca
       61 cagccctact gctcggcggg cttgtcttcg gcgggggcgt cggtgggggc cgccgacgtg
      121 gcggtggcgg cggcctccgg ggcaggctcg tccttcttcc agaacatgag cttgctcttc
      181 agattgctgc cgaaagtgcg aatggcggcg ccgattccgg caactccggc cagaactgcg
      241 gcgagggcag cgctgacgct gccggggtcc agataggcaa aaatgtgcat tcgtgatcct
      301 ctcgtgggcg ggatcgaccg ttgcctgact ttcctgagca ggccccgacc gaccgcgaaa
      361 tgttactcaa tcgtgaccag agggtccccg gacgcgacgg catcgccaac ggaaatgctg
      421 accttggtga cctttccggc cttgtgagcg gtgatctcgt tttccatctt catcgcttcg
      481 atcacgcaga tcaggtcgcc ttcagccacc tcggcgcctt cggcgacttc gagcttgaag
      541 atcgatccct ggagcggtga ggcaagcacg gggccgcctc cgccgcctcc gccaccggcg
      601 cgcttctcgc gcttcggctt gggtcctccg gctccgggcg cggcgccggc ggcggcaaag
      661 ccggtcgctt cgccgatcac cttgacgtca aagcgcttgc cggaaacctc gaccaggtag
      721 tcgcgggcaa ccttttcggc ttcgccgtca gccggctcgg ggatcgaagt gtcctcgggg
      781 gcggtggtct tcagccactt cttgtcttcg gtcaggtcct tgcaggtctc gccacgggcc
      841 cactgttcgg tccggaggat cgtcttgtgg aacgggatca gggtcgagag cggcgcgatc
      901 tcgtactcgt cgagcgcccg cagcatccgc ctggtcgcgg cctcgcggtc cttgtcccag
      961 acgatcagct tggcgaccat cgggtcgtac atcggggtga cctcggaacc ggccttgacg
     1021 ccggaatcga cccggacgcc cggaccggaa ggctcgtcgt agacctcgat cgggcccgga
     1081 gcgggggcga agttcatgtg ggccttctcc gcgttgatgc ggcattcgat cgcccagcca
     1141 tcgatcgtta cgtcttcctg cttgaggctc agttcctcgc cgtaggcgat cttgatctgc
     1201 tcccggacga tgtcgacccc ggtgatctcc tcggtcacgc agtgctcgac ctggacccgg
     1261 gtattcatct cgagg
//
LOCUS       DAOQFI010000691         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_82279_length_1275_cov_2.609016, whole genome shotgun sequence.
ACCESSION   DAOQFI010000691 DAOQFI010000000
VERSION     DAOQFI010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82279_length_1275_cov_2.609016"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLE93_10600"
     CDS             complement(<1..845)
                     /locus_tag="PLE93_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH87542.1"
                     /translation="MSGLSRGAPDKVTIKVRGDGSPLAETGSEVEYREEEKAKAAAAQ
                     AAAGRAGNEELFERARRVLPGGVNSPVRAMGQVGIDPVFIESARGAEVLDRQGRRYLD
                     WVSSWGPMILGHADPEVLAAIEEAAGRGTSFGAATELEVELAEEVAERMPSVEMIRMT
                     SSGTEAAMTAARLARAATGREVLVKFAGAYHGHSDGLLAEAGSGVATHGIPSGPGIPE
                     AQAAGTVVIPWNDREAVAEALEKHKVAALFAEPVAANMGVVPPEEGYLEFLRQATARA
                     GAMLV"
     gene            complement(871..>1275)
                     /locus_tag="PLE93_10605"
     CDS             complement(871..>1275)
                     /locus_tag="PLE93_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNH87543.1"
                     /translation="YAAKYASAFYGPFREAAESTPAFGDRRGYQMDPANSDEAVREAK
                     LDLEEGADALMVKPAGHYLDIVRRVKDETGAPVAAYQVSGEYSAIRAAGANGWIDERA
                     AALESLVSIRRAGADFIVTYFAREAAAWLAES"
BASE COUNT          178 a          479 c          405 g          213 t
ORIGIN      
        1 aagaccagca tcgccccggc ccgggcggtc gcctgccgca ggaattcgag gtagccctcc
       61 tccggaggca cgactcccat gttggccgcc acgggttcgg cgaaaagcgc cgccaccttg
      121 tgcttctcga gggcctcggc caccgcctcg cggtcattcc aggggatgac cacggtgccg
      181 gctgcctggg cttcgggaat tccggggccg gaaggaatgc cgtgggtcgc gacccccgag
      241 ccagcttccg ccagcaaccc gtcggagtgg ccgtggtagg cgccggcgaa cttgaccagc
      301 acctcgcgac ccgtggccgc ccgggccagc cgggccgccg tcatcgcggc ctcggttccc
      361 gaactggtca tgcggatcat ctcgaccgaa ggcatccgct ctgccacctc ttcggccagt
      421 tccacctcga gctcggttgc cgcgccaaag ctcgttcccc gtccggccgc ctcctcgatc
      481 gccgccagga cctcggggtc ggcgtggccg aggatcatcg gcccccagga actcacccag
      541 tcgagatagc ggcggccttg ccggtcgagc acttccgcgc ctctggcgga ttcgatgaag
      601 acggggtcga tcccgacctg gcccatcgcc cggaccggtg aattcacccc tcccggcagg
      661 acccggcggg cccgctcgaa tagctcctcg ttgccggccc gtccggcagc cgcctgtgcc
      721 gccgcggctt tcgccttctc ctcttcccgg tattcgacct cggaaccggt ctcggccagc
      781 gggctgccgt cgccgcgtac cttgatcgtg accttgtcgg gggcgccccg gctcagacct
      841 gacatccgct tctcctcaca atgaaactcc tcacgattca gccagccacg cggctgcttc
      901 cctggcgaag taggtgacga tgaaatctgc tccggcccgc cggatcgaga ccagtgattc
      961 aagggccgcg gcccgctcgt cgatccagcc gttggccccg gccgccctga tcgccgagta
     1021 ctcgccggac acctgatagg ccgcgaccgg ggcgccggtc tcgtccttga cccggcggac
     1081 gatgtcgagg tagtggccgg cgggtttgac catcagcgcg tcggcgccct cctcgaggtc
     1141 gagcttcgcc tcgcggaccg cttcgtccga gttggccggg tccatctggt agccccgacg
     1201 atcaccgaaa gcgggggttg attcggccgc ctcgcgaaac ggcccgtaaa acgccgacgc
     1261 gtacttggcg gcgta
//
LOCUS       DAOQFI010000692         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_82898_length_1270_cov_2.925103, whole genome shotgun sequence.
ACCESSION   DAOQFI010000692 DAOQFI010000000
VERSION     DAOQFI010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82898_length_1270_cov_2.925103"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLE93_10610"
     CDS             complement(<1..540)
                     /locus_tag="PLE93_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNH87544.1"
                     /translation="MSGEDAYAKAGVSQSDADLAVSRLVAALATANIGRPTRQVDLKG
                     HYAAVMRLDNRTGIALSTDGVGTKLLVAEELGRFDTVGIDCIAMNVNDIICVGAEPVA
                     MLDYLAVEKADPEMCEQIGVGLARGAELAGIEIPGGELAQIGDLVKGFDIAGACFGTV
                     ALDSIIDGSAVAPTNVVIGL"
     gene            complement(568..>1270)
                     /locus_tag="PLE93_10615"
     CDS             complement(568..>1270)
                     /locus_tag="PLE93_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNH87545.1"
                     /translation="DPDELDPELRKSFEVALHGGAALALLITRRGEIRDEIGEFDLRR
                     ASVLLLSFLPAAAVGYGFERVIESRFGGPRGTATGLILGGLAMALADRRPQERGRGEA
                     DWKDGLALGIGQAAALIPGVSRNGATLTAARLRRFSRDQANLLSRTVALPVILGAVGL
                     KGVRLARRGISRRQARWLAIGTVTSFASTMAATDLIRMVERDRALAPYAAYRIAFALL
                     ILARLAADRGDRAAE"
BASE COUNT          208 a          443 c          432 g          187 t
ORIGIN      
        1 caggccgatc accacattgg tcggggcgac cgccgatccg tcgatgatcg agtcgagggc
       61 gaccgtgccg aagcaggcgc cggcgatgtc gaaacccttg accaggtccc cgatctgggc
      121 gagctcgccg ccggggatct cgattccggc cagttcggcg ccccgggcca ggccgacccc
      181 gatctgctcg cacatctccg ggtcggcctt ctcgaccgcg aggtaatcga gcatggcgac
      241 cggttcggct ccgacgcaga tgatgtcgtt cacgttcatc gcgatgcagt cgatcccgac
      301 cgtgtcgaaa cggccaagtt cctcggcgac cagcagcttg gtcccgaccc cgtccgtcga
      361 aagcgcgatc ccggtgcggt tgtcgagccg catcaccgcg gcgtaatggc ccttgagatc
      421 gacctgccgg gtgggccggc cgatgtttgc ggtggccagg gcggcgacca gccggctgac
      481 cgcgaggtcc gcgtcggact ggctgactcc cgccttcgcg tatgcgtctt cgccgctcat
      541 cgcagttgat tcaacattag aaggcggtca ctcggcagca cggtcaccgc gatcggcggc
      601 cagtctcgcc aggatcagca gggcgaaagc gatccggtag gccgcgtaag gggcgagtgc
      661 ccggtccctt tcgaccatcc ggatcagatc ggtcgcggcc atcgtcgagg caaaggaagt
      721 aacggtgccg atcgccagcc agcgcgcctg ccggcgtgag atgccgcgcc gggcaagccg
      781 tacccccttc aggccgaccg ccccgaggat caccggcagg gcgacggtgc gggagagcag
      841 attggcctga tcacgggaga agcggcgcaa ccgggcggcg gtcagggtgg cgccgttgcg
      901 tgagacgccg ggaatcagcg ccgccgcctg gccgatcccg agggcgagcc cgtccttcca
      961 gtccgcttcc ccccggcccc gctcctgggg ccggcgatcg gccagggcca tcgccagccc
     1021 cccgaggatc agcccggtcg cggtgccccg cggcccgccg aagcgggatt cgatgacccg
     1081 ttcgaatcca tagccgacgg cagccgccgg caggaaggaa aggagcagga ccgaagcgcg
     1141 acggaggtca aattcaccga tttcatcccg gatctctccg cgccgggtaa tcaacagcgc
     1201 cagcgctgcg ccgccgtgaa gggcgacctc gaagctcttg cgcagttccg ggtcgagctc
     1261 atccggatcc
//
LOCUS       DAOQFI010000693         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_82923_length_1270_cov_2.577778, whole genome shotgun sequence.
ACCESSION   DAOQFI010000693 DAOQFI010000000
VERSION     DAOQFI010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82923_length_1270_cov_2.577778"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLE93_10620"
     CDS             complement(<1..572)
                     /locus_tag="PLE93_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH87546.1"
                     /translation="MGAKITGERVMTPEGGFNPTWQRHVAAYKLAEPFFGPGQLLDLG
                     CGVGHSFDLLAPRKTIGVDIDPGALEGQERETVVADMRRIPFEDGSFDSVLSVHSVEH
                     VPDPEKVVAEVSRVLRPEGVAVFVTPNRLTFGRPDEIIDPFHFIEFDAAQLEALCRAG
                     FREVETSGIFGTSRYMSIHDEERDRLDRLLG"
     gene            658..>1270
                     /locus_tag="PLE93_10625"
     CDS             658..>1270
                     /locus_tag="PLE93_10625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNH87547.1"
                     /translation="MPWILVGVLTLTGLLFRVIVFGESGFGDELSTLWIVRNNDLAGV
                     VSIVHTDAEITPPLYFLLAKITSVFGPTISSVRLPSLLAGVGSLPLVYAAGRRLIGSR
                     GALAATLLATVSPFLVYFSAQARNYAVMIFLLLLATLLILEATAEGGRWWHWAGWSLV
                     AALALFSQYTAGFVILAQVLWVFWFFPAQRLRMAGFAVLAGLLF"
BASE COUNT          174 a          440 c          412 g          244 t
ORIGIN      
        1 ccgagcagcc ggtcgaggcg gtcgcgttcc tcgtcgtgga tcgacatgta ccggctggtg
       61 ccgaagatgc cgctggtttc gacctcgcgg aacccggccc ggcagagtgc ctccagctgg
      121 gccgcatcga actcgatgaa gtggaacgga tcgatgatct cgtccggccg gccgaaggtg
      181 agccggttgg gagtcacgaa aaccgcgacg ccttccggcc gcagcacccg ggagacctcg
      241 gccacgacct tctccggatc cggcacgtgc tcgaccgaat ggaccgaaag gaccgagtcg
      301 aaactgccgt cctcgaaggg aatccggcgc atgtcggcga ccacggtctc gcgctcctgg
      361 ccttccaggg cgccggggtc gatgtcgact ccgatcgttt tccgcggggc cagcaggtcg
      421 aagctgtgcc cgaccccgca gcccaggtca agcagctggc cggggccgaa gaagggttcg
      481 gccagcttgt aggcggcgac gtgccgctgc caggtcggat tgaaccctcc ctcgggcgtc
      541 atgacccttt ccccggtgat cttggcgccc attggggcga atcttgacag gaaggacgat
      601 caggcccgta gtaacctgcg ccctcctgtg gacggagcgg aagggcagct cgggcgtatg
      661 ccctggatcc tcgtgggggt cctcaccctc accggtctcc tgttccgcgt gatcgtgttc
      721 ggcgagtcgg gtttcggcga tgagctatcg accctttgga tcgtccgcaa caacgatctg
      781 gccggggtcg tctcgatcgt tcacaccgac gccgagatca ccccgccgct ctacttcctg
      841 ctggccaaga tcacctcggt tttcggtccg accatctcct cggtcaggtt gccgtcactc
      901 ctggcggggg tcggaagcct gccgctcgtc tacgccgccg ggcgccgcct gatcggcagc
      961 cggggtgccc tcgccgcaac cctgctcgcc accgtctcgc ccttcctcgt ctacttctcg
     1021 gcccaggccc gcaactacgc ggtgatgatc ttcctgctgc tgctcgccac cctgctgatc
     1081 ctcgaggcga ccgcggaagg cgggcgctgg tggcattggg ccggctggtc gctggtcgcc
     1141 gccctggcgc tcttcagcca gtacacggcg ggcttcgtga ttctcgccca ggtcctctgg
     1201 gtgttctggt tcttccccgc ccagcggctg cgaatggccg gctttgcggt tctggccggc
     1261 ctgctgttca
//
LOCUS       DAOQFI010000694         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_83815_length_1263_cov_2.271523, whole genome shotgun sequence.
ACCESSION   DAOQFI010000694 DAOQFI010000000
VERSION     DAOQFI010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83815_length_1263_cov_2.271523"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLE93_10630"
     CDS             <1..440
                     /locus_tag="PLE93_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alkanal monooxygenase"
                     /protein_id="HNH87548.1"
                     /translation="YSFAHHFSAGNTEAAVQLYRSRFEPSAFLEEPKVMIGVGVICAE
                     TDEEARWLAGPSRLAFARLRSGRPGRFPTPEEAAAHDFTPDEESVVASVAKGHIFGSP
                     ETVQAGLVDLIERTGADELIISTMVYEQKDRLRSYELVSGIAL"
     gene            551..>1263
                     /locus_tag="PLE93_10635"
     CDS             551..>1263
                     /locus_tag="PLE93_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87549.1"
                     /translation="MQTRLPQTSSGRARGFSLAAALIVAAFVIALASWARPADALTVD
                     QTQSKIDAINSSVDDLKTQVAADNRKIDALIGELSGLRARASELEAQLAAKQAELDKV
                     EAALEREKEHLAQVRARLHRALKILEEQLVAVYMNGSPDVEDMVLASSDFGDLVSSAG
                     YADAIQDRNESVITRVKDLRDQIEAIVQRMKVQEKKLQAARDEIAAETKQAQEARDAV
                     EAQKAEFEASSAARQAKIDA"
BASE COUNT          232 a          429 c          389 g          213 t
ORIGIN      
        1 cctattcctt cgcccatcac ttcagtgccg gcaacacgga ggcggcggtc cagctctacc
       61 gctcccgctt cgagccgtcc gcctttctgg aagaaccgaa ggtgatgatc ggggtggggg
      121 tgatctgcgc cgaaaccgac gaggaagccc gctggctggc cggtccctcg cggcttgctt
      181 tcgcccggct ccggagcggc cgaccgggcc ggttccccac acccgaagag gcggcggcgc
      241 atgatttcac cccggatgag gaatcagtcg tcgcttccgt ggccaagggt catatattcg
      301 gcagtcccga aaccgtgcag gccgggctgg tcgacctgat cgagcggacc ggagccgacg
      361 agctgatcat ctcgaccatg gtttacgagc aaaaggaccg gctcaggtcc tacgaactcg
      421 tctctggcat cgcgctgtag agccgttttc cttcaattgc acggtatttc ccacggaccg
      481 tcgggacctc cggcgccggg ctttctcccg ctaccttgct tcttccgcct gcaccttccg
      541 gcggaccctc atgcagacca gactcccgca aacatccagc ggccgtgccc gcggcttcag
      601 ccttgctgcg gccctgatcg tggctgcttt cgtgattgcg cttgcctcct gggcacgccc
      661 ggccgacgcg ctgacggttg accagaccca gtcgaagatc gacgcaatca acagctcggt
      721 cgacgacctc aagacccagg tcgctgccga caaccgcaag atcgacgccc tgatcggcga
      781 gctttccggg ctgcgggccc gggcctccga gcttgaggcc cagctggccg ccaagcaggc
      841 cgaactcgac aaggtggagg ctgcgctcga gcgtgagaag gaacacctcg cccaggttcg
      901 cgcccggctt caccgggccc tgaagatcct cgaagagcag cttgtcgccg tttacatgaa
      961 cggttcgcca gatgtcgagg acatggttct cgcttcctcg gactttggcg atctcgtttc
     1021 cagcgccgga tacgccgacg cgatccagga ccgcaacgaa tcggtgatca cccgggtcaa
     1081 ggacctccgt gaccagatcg aggcgatcgt ccagcggatg aaggtccagg agaagaaact
     1141 tcaggccgca cgcgacgaga tcgcggccga aacgaaacag gcccaggaag cccgcgacgc
     1201 ggtcgaggcg cagaaggccg aattcgaggc atcgagcgcg gcccgccagg ccaagatcga
     1261 cgc
//
LOCUS       DAOQFI010000695         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_84098_length_1261_cov_1.897181, whole genome shotgun sequence.
ACCESSION   DAOQFI010000695 DAOQFI010000000
VERSION     DAOQFI010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84098_length_1261_cov_1.897181"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..910)
                     /locus_tag="PLE93_10640"
     CDS             complement(74..910)
                     /locus_tag="PLE93_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HNH87550.1"
                     /translation="MSPRTPILAKVKTTEPLTPHMVRIVLQSEGLRGFPIGEFTDHYV
                     KLQLPPPGATYSPPFDPVRIRESLAKDQWHRQRTYTVRGADPDAGEITIDFVTHGSGL
                     AGPWARDARPGDPVQLVGPGGAYTPDPDADWHLLAGDDAAIPAIAVALRRIPAGIPAV
                     VVLEVENEAEQLSLVETEGLTTEGELQLIWLHRDGAEHTAEPRILNVIEQLELPGGRG
                     QAFVHGEAGMVREVRRHLVKDRGMELEDLSATGYWKFRRTEEGWREDKPEWKRLAEAD
                     VS"
     gene            complement(947..>1261)
                     /locus_tag="PLE93_10645"
     CDS             complement(947..>1261)
                     /locus_tag="PLE93_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87551.1"
                     /translation="TVSETQRGYILPARHFASDSRNRALPPMGLRLRLKAGYRLGRFS
                     GQALAILRALKKYGLIVADNGSDMFISGTPDRRWNDDVLDQLKTVPARAFQAVVTGRI
                     HR"
BASE COUNT          195 a          398 c          443 g          225 t
ORIGIN      
        1 cgggcagtgc gccccgacga cgtttcgggc cgaggagggg tgtactgccc ggaccgaccc
       61 taaacgccca tggtcaacta acgtcggcct cggccaaccg tttccattcc ggcttgtcct
      121 cgcgccaccc ctcttcggtc cgccggaact tccagtaacc ggtcgccgaa aggtcttcga
      181 gttccatgcc ccggtccttg accaggtgcc tccggacctc gcgcaccatg ccggcctcgc
      241 cgtggacgaa ggcctggccc cggccgcccg gcagttcgag ctgctcgatc acgttgagga
      301 tccgcggttc ggccgtgtgt tcggcaccgt cgcggtggag ccagatgagc tggagctcac
      361 cttccgtggt gagaccttct gtttcgacca gcgatagctg ctcggcttcg ttctcgacct
      421 ccagtaccac cacggccggg atcccggccg gaatcctccg cagggcgaca gcgatcgccg
      481 gaatcgcggc atcatcacca gccagaaggt gccagtcggc atcgggatcg ggtgtgtagg
      541 cgcctccggg cccgaccagc tggacgggat caccgggcct ggcgtcgcgg gcccaggggc
      601 cggccagccc cgatccgtgg gtcacgaagt cgatcgttat ctcgccggca tcggggtcgg
      661 cgccgcgaac ggtgtaggtc cgctggcggt gccattgatc cttcgccagg ctctcccgga
      721 tccggaccgg gtcgaagggc ggtgagtagg tggcaccggg aggcgggagc tgaagcttca
      781 cgtagtggtc tgtgaattca ccgatcggga atccccggag cccctccgac tggaggacta
      841 tgcgaaccat gtggggagtg agcggttcgg tcgtcttgac tttggcgagg atcggggttc
      901 ggggactcaa gtgattcctg tcggtcgtgg caggctgggg agaggtctat cggtggatac
      961 ggccggtcac gaccgcctgg aaggcacggg ccgggaccgt tttgagctga tcgagaacgt
     1021 cgtcattcca gcgccggtcc ggggtgccgc tgatgaacat gtcggagccg ttgtcggcga
     1081 cgatcagacc gtattttttc agggcccgga gaatcgcgag ggcctgaccg ctgaagcggc
     1141 caagccggta gccagctttg agccgcaggc gaaggcccat cgggggcagc gcccggttgc
     1201 gcgaatccga tgcgaagtgc cgggcgggga ggatgtaccc gcgctgcgtc tccgagacgg
     1261 t
//
LOCUS       DAOQFI010000696         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_84583_length_1257_cov_2.292845, whole genome shotgun sequence.
ACCESSION   DAOQFI010000696 DAOQFI010000000
VERSION     DAOQFI010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84583_length_1257_cov_2.292845"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLE93_10650"
     CDS             complement(<1..221)
                     /locus_tag="PLE93_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87552.1"
                     /translation="MTRPEQKRAFGVAAGLPAEVCAPLAAACEEAGYASVWANDHPLA
                     KGLETLAEFAKGADGVELGVAVIALDRQSP"
     gene            complement(218..>1257)
                     /gene="rlmD"
                     /locus_tag="PLE93_10655"
     CDS             complement(218..>1257)
                     /gene="rlmD"
                     /locus_tag="PLE93_10655"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNH87553.1"
                     /translation="DEQWRYRNKVEYSFGEQDGELVLGFHPRGRWDEVLDIEDCHLSS
                     EATNRARNEVLAWARAEGLNAYDPRDHRGILRNLVVREGRRTGQVQTRLVTSRANFPK
                     PPVDLHTIIDGPSGSTSGPTGVLGEEVLRERIFDLDIELSHDAFFQTNTEMAEKLYGL
                     AAEYAGLTGNEKLFDLFCGVGTIGLSMARQAGQVWGLEIVPEAIENAKRNAKANGITN
                     ARFVAANARTGMRPLIEEAGRPDVVTIDPPRAGLSAKIVRRLIECEARRIVYISCNPT
                     TLAPNAAQLVEAGYRLTRVRPVDMFPQTPHIECVAQFDLVGEVKYVESPTDRKRRERD
                     ERRQAAKEAEG"
BASE COUNT          183 a          396 c          448 g          230 t
ORIGIN      
        1 gggctctgcc ggtcgagggc gatcacggcg accccgagct cgaccccgtc ggcacccttg
       61 gcgaattcgg ccagcgtttc gagccccttg gccaggggat ggtcattggc ccagaccgag
      121 gcgtagccgg cctcctcgca ggcggcggcc aggggggcgc agacctcggc cggaagtccg
      181 gcggcaactc cgaaagcgcg cttctgttcg ggacgggtca accttcagcc tccttcgcgg
      241 cctggcgccg ctcgtctcgt tcacgacgtt tgcggtcggt gggggattcg acgtacttca
      301 cctcgccgac cagatcgaac tgggcgacgc actcgatgtg cggggtctgg ggaaacatgt
      361 caaccggcct cacccgggtc agcctataac cggcctcgac cagctgggcg gcgttcgggg
      421 ccagcgtggt cgggttgcag gagatgtaaa cgatccggcg ggcttcgcat tcgatcaggc
      481 ggcgcacgat cttggccgag agcccggccc ggggcggatc gatcgtcacc acatcgggcc
      541 ggccggcttc ctcgatcagg gggcgcatcc cggtgcgggc gttggcggcg acgaaacggg
      601 cgttggttat gccgtttgcc ttcgcgttcc gcttcgcgtt ctcgatcgcc tcgggaacta
      661 tttccaggcc ccagacctga ccggcctgcc gggccatgct gagcccgatc gtgccgactc
      721 cgcagaagag gtcgaagagt ttctcgttgc cggtcagccc ggcgtactcg gcggcgaggc
      781 cgtagagctt ctcggccatt tcggtattgg tctggaagaa ggcgtcgtgg gagagctcga
      841 tgtcgaggtc gaagatccgc tcccgcagga cttcctcgcc gagtaccccg gtgggtccgg
      901 aagtcgaacc ggagggaccg tcgatgatcg tgtgaaggtc gactggcggc ttggggaagt
      961 tggcgcgcga ggtgaccagc cgggtctgga cctgcccggt ccggcggcct tcgcggacga
     1021 ccaggttgcg caggatgccg cggtggtcac gggggtcgta ggcgttgagg ccttcggccc
     1081 gcgcccaggc gaggacttcg ttgcgagccc ggttggtcgc ttcggatgag aggtgacagt
     1141 cctcgatgtc cagcacctcg tcccagcgac cacgggggtg aaagccgagc accagctcgc
     1201 cgtcctgttc gccaaatgag tattcgacct tgttgcggta tcgccactgt tcgtcgg
//
LOCUS       DAOQFI010000697         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_84739_length_1256_cov_1.589509, whole genome shotgun sequence.
ACCESSION   DAOQFI010000697 DAOQFI010000000
VERSION     DAOQFI010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84739_length_1256_cov_1.589509"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PLE93_10660"
     CDS             <1..770
                     /locus_tag="PLE93_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH87554.1"
                     /translation="CLKLGADIVVNTDADNQYSGSDIPKLVAPILAGDAEMVVGDRKV
                     AEIDHFSPAKKVLQKFGSWVVRQLSNTEVTDTTSGFRAYNREAALQLLVVDNFTYTLE
                     SLIQAGKGNVAVGEVTISTNEKTRESRLFGSTSAYVRRNGLAILRIYTRYEPLKVFSL
                     LGLIAAVAAIAAWMPFLIDWIVNGDRDGHIQSLLLGAVLFIVSIQLFALGIIGDLLAG
                     QRVMTQRVYERVRRIELELEVEPSHRLDPPRDRSAVQ"
     gene            775..>1256
                     /locus_tag="PLE93_10665"
     CDS             775..>1256
                     /locus_tag="PLE93_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87555.1"
                     /translation="MSDSPEKTESSTRAYGRSVGLLTIGVGLTGIFTYAYFLIASHDL
                     SKDAYGEITVLWSAVFITVSTLYRPVDQLLSRHISEHIERGETDVGPVRVAAKIQICL
                     AIGFAILGGKFLDYSMLNKASPGDGEALGMTLVEYGVGITVSMVMVIIFNQLAEEQAR
                     A"
BASE COUNT          222 a          429 c          369 g          236 t
ORIGIN      
        1 cctgtctcaa gctcggggcc gacatcgtcg tcaacaccga cgccgacaac cagtactccg
       61 gaagcgacat tccgaagctg gtcgcgccga tcctggccgg tgatgccgag atggtggtcg
      121 gggaccgcaa agtggccgag atcgatcact tctcaccggc caagaaggtt ctccagaagt
      181 tcggcagctg ggtcgtgcgc cagctttcca acaccgaggt caccgatacg acttccggtt
      241 ttcgcgccta caaccgggaa gcggcgcttc agctgctggt cgtcgacaac ttcacctaca
      301 cgctcgaaag cctgatccag gccggcaagg ggaacgtcgc ggtcggcgag gtcacgatct
      361 cgaccaacga gaagacccgc gagtcccgcc ttttcggttc gacttccgcc tatgtgcgcc
      421 gcaacggcct ggcgatcctc cggatctaca cccgctacga gccgctcaag gtcttttcgc
      481 tgctcggctt gatcgccgcc gtggccgcca tcgcagcctg gatgccgttc ctgatcgact
      541 ggatcgtgaa cggcgaccgc gacggtcaca tccagtcgct gctgctcggt gcggtgctct
      601 tcatcgtctc gatccagctc ttcgcgctcg gcatcatcgg tgacctgctg gccggccagc
      661 gggtcatgac ccagcgcgtc tatgaaaggg tccgccggat cgagcttgag ctcgaagtcg
      721 agccgagcca tcgactcgat ccccctcgtg accgaagcgc cgtccagtaa tcacttgagc
      781 gattccccgg agaaaaccga atcttcgacc cgtgcctacg gccggtccgt cgggctgctc
      841 acgatcgggg tcggcctcac cggcatcttc acctacgcct acttcctgat cgcttcccat
      901 gacctgagca aggacgccta cggcgagatc accgtgctct ggtccgcggt cttcatcacc
      961 gtgtcgactc tctaccgccc ggttgaccag ctgctctccc ggcacatctc ggaacacatc
     1021 gaacggggcg agaccgacgt cggtccggtc cgggtcgcgg cgaagatcca gatctgcctg
     1081 gcgatcggct tcgcgatcct cggcggcaag ttcctcgact actcgatgct gaacaaggcg
     1141 agccctggcg acggcgaggc gctcggcatg accctcgtcg agtacggggt cggcatcacc
     1201 gtcagcatgg tcatggtcat catcttcaac cagctcgcgg aagagcaggc gagggc
//
LOCUS       DAOQFI010000698         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_85040_length_1253_cov_3.291319, whole genome shotgun sequence.
ACCESSION   DAOQFI010000698 DAOQFI010000000
VERSION     DAOQFI010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85040_length_1253_cov_3.291319"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1253)
                     /locus_tag="PLE93_10670"
     CDS             complement(<1..>1253)
                     /locus_tag="PLE93_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH87556.1"
                     /translation="AEAFEAAAEGNVILTTATASGKSLAFNLPPLNAIAADPKARALY
                     LYPTKALAQDQARALARLGSPNLRLAIYDGDTPRDERPAIRRQSNLVLTNPDMLHVGI
                     MPHHRGWGDFLANLDWVVVDEAHTYRGVFGSHVANVLRRLRRLARAYGREPRFVMASA
                     TIANPAELAERLTGSPARLIDEDGAPRAGREIRIWNPPLVNPQVGARRSALAEAADLM
                     ADLVSHEVRTICFLKSRKGIELIRKFAADRLTEKGRPDLAERIAPYRAGYTPAQRREI
                     ERKLVSGELLGVASTDALELGIDIGGLDAAICVTFPGTVAGLKQMWGRAGRRERGLAV
                     YIAGPDGLDQYFCRHPDEFLERPVEAAILDHLSPEIRDQHLIAAAYELPLTSEDEEFF
                     GSGLEDAGQALVLRGELRKAGKGLI"
BASE COUNT          175 a          417 c          468 g          193 t
ORIGIN      
        1 aatcagcccc ttgccggcct tgcgcaactc gcccctgagg accagggcct ggccggcgtc
       61 ctccaggccg gagccgaaga actcctcgtc ctcggaggtg agcggcagtt cgtaggcggc
      121 ggcgatcagg tgctggtcac ggatctcggg tgagaggtga tcgaggatcg ccgcctcgac
      181 cggccgctcg aggaactcgt ccgggtggcg gcagaagtac tggtccaggc cgtccgggcc
      241 ggcgatgtag acagcgagcc cgcgttcacg gcgtccggcc cggccccaca tctgcttcag
      301 gccggccacg gtgccgggaa aggtgacgca gatcgcggcg tcgaggccgc cgatgtcgat
      361 gccgagctcc agcgcgtcgg tcgaggcgac cccgagcagc tcgccggaaa cgagcttccg
      421 ctcgatttcg cggcgctggg ccggggtgta ccccgcccgg tagggagcga tccgctcggc
      481 caggtcaggc cggccctttt cggtcagccg gtcggcggcg aacttgcgga tcagctcgat
      541 ccccttgcgg gacttcagaa agcagatcgt ccgtacctcg tgggagacca ggtcggccat
      601 caggtccgcc gcctcggcca gggcggagcg tcgcgcgccg acctgtggat tgacgagtgg
      661 cgggttccag atccggatct cccggccggc tcgcggggcg ccgtcttcgt cgatcaggcg
      721 agccgggctg ccggtcagcc gctcggccag ctccgcgggg ttggcgatcg tcgccgaggc
      781 catcacgaag cggggctccc tgccgtaggc ccgggccagc cgcctgagcc gtcgcagcac
      841 gttcgcgaca tgcgagccaa agactccccg gtaggtgtgg gcctcgtcga caaccaccca
      901 gtcgaggttg gcgaggaagt caccccagcc gcggtggtgc ggcatgatcc cgacgtggag
      961 catgtccggg ttggtcagga ccaggttgct ctggcggcga atcgccgggc gctcgtcccg
     1021 cggggtgtca ccgtcataga tcgccagtcg caggttcggt gaaccgagcc gggccagggc
     1081 gcgggcctgg tcctgggcga gcgccttggt cgggtagagg tagagcgccc gcgccttggg
     1141 gtcggctgcg atcgcgttga gcggaggcag gttgaaggcc agcgacttgc cggaagcggt
     1201 cgcggtggtc aggatcacgt tgccctcggc cgccgcctcg aaggcctcgg cct
//
LOCUS       DAOQFI010000699         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_85099_length_1253_cov_2.353088, whole genome shotgun sequence.
ACCESSION   DAOQFI010000699 DAOQFI010000000
VERSION     DAOQFI010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85099_length_1253_cov_2.353088"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLE93_10675"
     CDS             complement(<1..395)
                     /locus_tag="PLE93_10675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH87557.1"
                     /translation="MLQPGNSTASRSRLLSGGMILAALAAVLTAFAVLAAPARSADPY
                     TVESNIPYAPANAQAGSNLLDVYVPKADSAKPRPVFVWIHGGGWFTGDKAGGTMEYKA
                     KTLVDNGFIFVSVNYRLSPDLAGPKALSKG"
     gene            complement(450..>1253)
                     /locus_tag="PLE93_10680"
     CDS             complement(450..>1253)
                     /locus_tag="PLE93_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNH87558.1"
                     /translation="SDLTEAGRSQAAHESGSLGIAISEAVEVAAGSEDANYALGSVLN
                     HVCLHQTVIGQEAIAAMEMAGEYPDVVVGCVGGGSNFAGLINPFLRENIRNGKTTRFL
                     GAEPAACPTLTRGKYAYDFGDTVGMTPLMPMYTLGHDFVPAPVHAGGLRYHGDAPSLC
                     AFVHEGFVEATAVPQGAAFEAALLFARTEGIIPGPEPAHAIRAVIEEAEAAKQAGEER
                     VILLGLSGHGHFDMSAYQAYLSGELEDLELDQEELDAAIERLPEAPAIA"
BASE COUNT          219 a          392 c          431 g          211 t
ORIGIN      
        1 cccttcgaga gcgccttcgg gccggccaga tcaggggaaa gccggtagtt gaccgaaacg
       61 aagatgaatc cgttgtcgac cagggtcttc gccttgtatt ccatggttcc accggccttg
      121 tcaccggtga accagccgcc accgtgaatc cagacgaaga ccggccgcgg tttggccgaa
      181 tcggccttcg ggacgtagac gtcaagcagg ttggagccag cctgggcgtt ggcgggcgcg
      241 tagggaatgt tggattcgac cgtgtaggga tcggccgacc tcgcaggcgc cgccaggacc
      301 gcgaaagcag tcaaaacggc cgccagagcg gcgaggatca tgcctccgga aagcaggcgc
      361 gatcgactgg ctgtggagtt ccccggctgg agcattcggg gtagttaaca acctttaccg
      421 gtcgggggtg cgcccggtcc cgggaaattt caggcgatcg ccggtgcctc cggcaggcgc
      481 tcgatcgcgg cgtccagttc ctcctgatcg agttcgagat cctcaagctc tcccgacagg
      541 tacgcctgat aggcggacat gtcgaaatga ccgtggccgg aaagcccgag caggatcacc
      601 cgctcctcac cggcctgctt cgccgcttcg gcttcctcga tgacggcgcg gatcgcatgg
      661 gccggttccg ggccgggaat gatcccttcg gtgcgggcga agagcagcgc cgcctcgaag
      721 gccgcaccct ggggaacggc ggtcgcctcg acgaaaccct cgtgaacgaa ggcgcagagg
      781 ctgggggcat caccgtgata cctgaggcct cccgcgtgaa cgggtgcggg cacgaagtca
      841 tgaccgagcg tgtacatcgg catcagcgga gtcatgccta cggtgtcacc gaagtcatag
      901 gcgtacttgc cccgggtcag ggtcgggcag gcggccggtt ccgcgccgag gaagcgggtc
      961 gtcttgccgt ttcggatgtt ctcgcgcagg aaggggttga tcaggccggc gaagttcgag
     1021 ccgccaccga cgcagccgac caccacatcc gggtattcgc cggccatctc catcgcggcg
     1081 atcgcttcct ggccgatcac ggtctggtgg aggcagacgt gattgaggac ggaacccagg
     1141 gcatagttgg cgtcctcgga gccggccgcg acctcgaccg cttccgagat cgcgatgccg
     1201 aggctgccgc tctcgtgagc tgcctgggaa cggcccgctt cggtcaggtc cga
//
LOCUS       DAOQFI010000700         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_85364_length_1251_cov_2.698161, whole genome shotgun sequence.
ACCESSION   DAOQFI010000700 DAOQFI010000000
VERSION     DAOQFI010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85364_length_1251_cov_2.698161"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PLE93_10685"
     CDS             complement(<1..777)
                     /locus_tag="PLE93_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNH87559.1"
                     /translation="MSRRNWPAVAILSLTLWLVGAGFAHAAGAPERGPKLDAKAWIVI
                     DARTGEPLAGHAVDRHLGMASTTKMMTGYLAVRKLPLREKVTAGKYDPDPAESLMGLE
                     PGQVVSVRDLLYGLFMLSGNDAAVTLAKTVSGSEAKFVKLMNRTALRLGLDDTHYDNP
                     VGLDGPSHYTSADDLAKLGRVVMGMPRLRKIAGSRVAKLTSYRPPLTIETTDSFLRDN
                     VWARGIKTGHTLGTGYTLASDGRKKATELIGAVIGTPSEAA"
     gene            complement(774..1094)
                     /locus_tag="PLE93_10690"
     CDS             complement(774..1094)
                     /locus_tag="PLE93_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNH87560.1"
                     /translation="MEEKKKNSYIARILAALALLGTILIIFVVISGVTGSDDDGTGKK
                     ANPARQQQKVKPKTKAKTYEVEEGDTLTTISRKTGIPVARLKQLNPDLDPQALQVGQE
                     LKLR"
BASE COUNT          186 a          408 c          423 g          234 t
ORIGIN      
        1 ggcggcttcc gagggggttc ctatcaccgc tccgatcaat tccgtggcct tcttgcgacc
       61 gtcggaggcg agcgtgtaac cggtacccag ggtgtggccg gttttgatcc cccgggccca
      121 gacgttgtcg cgcaggaagc tgtcggtcgt ctcgatcgtc agcggcggcc ggtagctggt
      181 cagcttcgcc accctggagc cggcgatctt ccgcaagcgc ggcattccca tgaccacccg
      241 gcccagcttc gccaggtcgt cggccgaggt gtaatggctc ggaccgtcga ggccgaccgg
      301 attgtcgtag tgggtgtcat cgagcccgag ccggagagcg gtgcggttca tcagcttgac
      361 gaacttcgcc tccgacccgg acacggtctt ggccagggtc acggcggcgt cgttgccgct
      421 cagcatgaag agcccgtaga gcaggtccct caccgatacc acctggccgg gctcgagccc
      481 catcagcgac tcggccggat cggggtcgta cttgccggcc gtcaccttct cgcggagcgg
      541 gagcttcctg accgcgaggt agccggtcat catcttggtg gtcgaagcca tgcccagatg
      601 gcgatcgacg gcgtggccgg cgagcggctc gccggtccgg gcgtcgatca cgatccaggc
      661 cttggcgtca agcttcggac cccgctccgg ggccccggca gcgtgggcga aacccgcccc
      721 gaccagccag agggtcaggc tcaggatcgc gactgccggc cagtttcgtc ggctcaacgc
      781 agcttgagtt cctggccgac ctgcagggcc tgcgggtcca ggtcgggatt cagctgcttc
      841 agcctggcca ccgggatgcc cgtcttgcgg gagatcgtcg tgagggtgtc gccttcctcg
      901 acctcgtagg tcttcgcctt cgtcttcggc ttgaccttct gctgctgcct ggccggattg
      961 gccttcttgc cggtgccgtc gtcatcgctg cccgtcactc cggagatcac gacgaagatg
     1021 atcaggatgg tgccgagcag cgccagagcg gcgaggatcc gggcgatata cgagttcttc
     1081 ttcttttctt ccatgatgga ccggagatga ctgggacagg ccagagagat tcgggccgga
     1141 aagttacaga tacggcggat cgaagcgcct gaaccggcgg gcgccgggcg cgatcacccg
     1201 gagacggacc agaccgcttc ccgcagttct tcggcggcag cggccggatc t
//
LOCUS       DAOQFI010000701         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_85426_length_1251_cov_1.862876, whole genome shotgun sequence.
ACCESSION   DAOQFI010000701 DAOQFI010000000
VERSION     DAOQFI010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85426_length_1251_cov_1.862876"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLE93_10695"
     CDS             complement(<1..244)
                     /locus_tag="PLE93_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNH87561.1"
                     /translation="MASQTDYASLIRAIPDFPEPGILFRDITPLLLDAAACRSAVSDL
                     AEWAAPHKPDLLLAAESRGFILGGALAQALDIGFIPA"
     gene            complement(254..676)
                     /locus_tag="PLE93_10700"
     CDS             complement(254..676)
                     /locus_tag="PLE93_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87562.1"
                     /translation="MPRTGLLLALAVVASLALPGVAGAAKNGTYVGRDEGKVKVVLKI
                     RNNRVTYFEGNPAGSCYGIGSTLVAFRYPSAAQRPSASSRIKADGVFSAVFKGSRFVS
                     FKDDQRTLTGKVRGNKVSGRMIVSGLCQVDQKFTATRK"
     gene            719..>1251
                     /locus_tag="PLE93_10705"
     CDS             719..>1251
                     /locus_tag="PLE93_10705"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNH87563.1"
                     /translation="MDFTTIDLPGLLLDTESAVAMKAHGSREAAVLIPIRNWPEAPRL
                     ILTERRADLRKHAGEISFPGGTSEPGDRDLEETALRESHEEIDLHPDQVEVIGAVPPV
                     GTFATSFRIQPIVGLVAEEAKVVANPDEVATILTFGFDELLDSYGMRRLVRRGVPIRT
                     PTFEMDRHLIWGATARIL"
BASE COUNT          210 a          423 c          413 g          205 t
ORIGIN      
        1 gggccgggat gaaaccgatg tccagcgcct gggccagtgc tccgccgagg atgaacccgc
       61 gtgattcagc ggccagcagg agatccggct tgtgcggcgc cgcccactcg gccaggtcgc
      121 tgaccgcgct ccggcaggca gcggcgtcga gcaggagcgg agtgatgtcg cggaagagga
      181 tgcccggctc gggaaaatcg ggtatcgccc ggatcagcga ggcgtagtcg gtctggcttg
      241 ccaaggcttc accttacttc cgggtcgcgg tgaacttctg gtccacctgg cagagtccgc
      301 tgacgatcat ccggccgctc accttgttgc ctctgacctt gccggtcagg gttcgctggt
      361 catccttgaa gctgacgaat ctggacccct tgaacacggc tgagaagacc ccgtctgcct
      421 tgatccttga actggcggag ggccgctggg cggccgacgg gtacctgaag gcgaccaggg
      481 tcgagccgat cccgtaacag gaaccggccg gattgccctc gaagtaggtc acccggttgt
      541 tcctgatctt caggacgacc ttgaccttgc cctcatccct gccgacatag gtcccgttct
      601 tcgcggcgcc ggcgaccccg ggcagggcga gggaagcgac cacggcaagg gccaggagca
      661 gtccggtacg gggcatcgcg cggcagctta ccggcggcgg gtgcggcatg atcccgctgt
      721 ggacttcacc accatcgacc ttcccggcct gcttcttgac accgagtcgg cggtggccat
      781 gaaggctcac ggcagccggg aagccgcggt actgatcccg atccggaact ggccggaagc
      841 gccgcggctg atcctcaccg agcgccgcgc cgacctgcgc aagcacgcgg gcgaaatctc
      901 ctttccgggc ggcaccagcg aacccgggga ccgcgacctc gaagaaacgg ccttgcggga
      961 atcgcacgag gagatcgacc ttcaccccga ccaggtcgaa gtgatcggag cggtgccgcc
     1021 ggtcggcacc ttcgcaacct ccttccggat ccagccgatc gtcggcctcg ttgccgaaga
     1081 agcgaaggtg gtcgccaacc cggatgaggt cgcgaccatc cttaccttcg gcttcgacga
     1141 gctgctcgac tcctacggca tgcggcgtct ggtccgccgc ggggtgccga tccgcacacc
     1201 caccttcgag atggaccgcc acctgatctg gggcgcgacg gcccgcattc t
//
LOCUS       DAOQFI010000702         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_85540_length_1250_cov_2.125523, whole genome shotgun sequence.
ACCESSION   DAOQFI010000702 DAOQFI010000000
VERSION     DAOQFI010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85540_length_1250_cov_2.125523"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLE93_10710"
     CDS             <1..688
                     /locus_tag="PLE93_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNH87564.1"
                     /translation="GLWLAEHQAKQVLRPAGIAGPAFLTATSPGACLDAAAGLSFPLV
                     LKLSAGSIRHKSELGAVRVGIEDEAALLREAEDLMSIAGQIHLEQGLAGEPSNDPPVY
                     LVEEMAGPGVELIVTARSDAVVPSLTVGLGGVWAEQLADSVVVPLPASPEQVRAALGR
                     LRGFEALLGRRGTPVTDLGALSELASGVGQLLLDYRDGEGRRFDLIELNPVLARPDGA
                     TALDALAHLA"
     gene            complement(720..>1250)
                     /locus_tag="PLE93_10715"
     CDS             complement(720..>1250)
                     /locus_tag="PLE93_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH87565.1"
                     /translation="SMGGVLMSDKVAEPFFDGRADYNNGYTFGGHPVGSAVALKSLEI
                     LERENVLENVNSNAPVAEEALKSLLDIPIVGQIRGTGHFWAIEVVRDRETKEPFKGKA
                     AEHLFKNVLSEALWHRGLLCRLDDRADPIIQIAPPLVAEPELFTEIAGILREGLEITV
                     EQAGAIAPAESSISPI"
BASE COUNT          166 a          425 c          441 g          218 t
ORIGIN      
        1 gggtttatgg ctggccgagc atcaggccaa gcaggtactg cgcccggcgg ggattgcggg
       61 gcccgccttt ctcactgcga cttccccggg cgcctgcctg gatgcggccg cggggctttc
      121 gttcccgctg gtcctgaagc tttccgccgg ttcgatccgg cacaagtccg agctcggggc
      181 ggtcagggtc gggatcgaag acgaggcggc gctgctccgc gaggccgagg atttgatgtc
      241 gatcgccggg cagatccatc tcgagcaggg cctggccgga gagccctcga atgatccgcc
      301 cgtctacctg gtcgaagaga tggccggccc cggagtcgag ttgatcgtca ctgcccgttc
      361 cgatgccgtg gtgccctcgc tcaccgtggg gctcggtggg gtctgggccg agcaactggc
      421 ggacagcgtg gtggttccgt tgccggcctc gccggagcag gtgcgggccg cgctcggccg
      481 gctgcggggc ttcgaagcgc tgctcggacg ccgcggaacc cccgtcaccg acctcggcgc
      541 gctttcggag ctggcctcgg gcgtcgggca gttgctgctc gactaccgcg acggggaagg
      601 ccgccgcttc gacctgatcg agctcaaccc ggtcctcgcc cggcccgacg gcgcaaccgc
      661 cctcgacgcc ctcgcccacc tcgcctgagg cgtcgtcgcc tggggcgttg tgtcgccggt
      721 cagatcgggc tgatcgaact ctcggcggga gcgatcgcgc cggcctgctc gaccgtgatc
      781 tccaggccct cccggaggat cccggcgatc tccgtgaaga gctccggctc ggcgaccagg
      841 ggcggggcga tctggatgat cgggtcggcc cggtcatcga gccggcagag caggccccgg
      901 tgccagaggg cctcggagag gacgttcttg aagaggtgtt cggcggcctt gcctttgaag
      961 ggctccttgg tctcgcggtc acgcaccacc tcgatcgccc agaaatgtcc ggtgccccgg
     1021 atctggccga cgatcgggat gtcaagcagc gacttcagcg cttcctcggc gaccggggcg
     1081 ttcgagttca cgttctcgag cacgttctcc cgctcgagga tctccagtga cttcagcgcc
     1141 accgccgagc cgaccggatg gccgccgaag gtgtagccgt tgttgtaatc ggcgcggccg
     1201 tcgaagaagg gttcggcaac cttgtcgctc atcagcaccc cgcccatcga
//
LOCUS       DAOQFI010000703         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_85589_length_1249_cov_5.255444, whole genome shotgun sequence.
ACCESSION   DAOQFI010000703 DAOQFI010000000
VERSION     DAOQFI010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85589_length_1249_cov_5.255444"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..630
                     /locus_tag="PLE93_10720"
     CDS             406..630
                     /locus_tag="PLE93_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87566.1"
                     /translation="MPSRDEIAATIPRLGRGARINLKLKYGGEVLGTLYGLFDDQVFF
                     EEEEIAPIDLDRIDDLLVRVHTEQPGGPAS"
     gene            complement(765..965)
                     /locus_tag="PLE93_10725"
     CDS             complement(765..965)
                     /locus_tag="PLE93_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87567.1"
                     /translation="MSGGGWRYSSGIVSQFGRYCRDCLHEKGVKTTSYSAAIEKLPSD
                     KQVEVEKLRNKWTVEDARRKPE"
BASE COUNT          252 a          414 c          333 g          250 t
ORIGIN      
        1 ccacctgagc gggcgatcgt gattcctgat caactcgacc gcaacccgct tgcgcgccgc
       61 ctcgtccggc ctgatcccgg cgtcatgctc ttccgtgcct tgaaccgcaa ctcccctcat
      121 ggggtccccc actccttaga cggcttggca ctcttcctat ccgggcgacc cgaaaattgc
      181 cagcggcaga aactacgccc acggaacctg gcccctacat tttcaaacca tcgaaaagcg
      241 tccatttgca gcgataaggg ccgcaggttc gaaaactctc cccccttcaa agtccgattt
      301 ctccgcaaat tgcgaactat tcggaatttc atgcaaattg catggaattt ctgattggtc
      361 tcagttcgga actcgcaaat tgggtcttct ggctaggctc cacccatgcc ctcgagagac
      421 gagatcgccg cgacaatccc ccgcctcggc cggggcgccc ggatcaacct gaagctgaaa
      481 tacggcggcg aagtgctcgg caccctctac ggcctcttcg acgatcaggt cttcttcgaa
      541 gaggaagaaa tcgcgccaat cgacctcgac cggatcgacg acctcctggt ccgggtccac
      601 accgaacaac ccggtgggcc ggcctcgtga gagttcgatg tccaaggtga gtgggtacac
      661 gccaatcgac gttcccgaac cgaacgagtc cttccgttcc ggcagcagtc cgttcggcac
      721 gcctcgagaa tgaccggctc ctcaagacac agccgcccgc ctgactattc cggcttcctc
      781 cgggcatcct cgaccgtcca cttattgcga agcttctcga cctcgacctg tttgtcggat
      841 ggaagcttct cgattgccgc cgagtagctc gtggttttga cccctttctc gtggagacaa
      901 tcccggcagt agcggccgaa ctgggagacg atgccggagg agtagcgcca gccgccaccg
      961 ctcaccgagt aacagcaggt ctgagcttca ccggtggtgg agcgggcacc gcaggggcat
     1021 cgggcgattt cacatcgaca acgggcagat cttctgtcgg ggtttccgtc gtagccagcc
     1081 gaataagtga atccggttcc ccaattacag gtgggacatt cacgaagtcc gatcggaatc
     1141 catcctccgg gcggctcgat tgcgacagcg acgtcattct ccgtggggac attgagaggc
     1201 agactcctcc aggcaccgca aaacgtaccg tgttgcgcac ctcgccatc
//
LOCUS       DAOQFI010000704         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_86043_length_1246_cov_2.626364, whole genome shotgun sequence.
ACCESSION   DAOQFI010000704 DAOQFI010000000
VERSION     DAOQFI010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86043_length_1246_cov_2.626364"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1044
                     /locus_tag="PLE93_10730"
     CDS             166..1044
                     /locus_tag="PLE93_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNH87568.1"
                     /translation="MPADPAATPDRVQAPFGRRLCEVAETRPSGGYRIFTLIDPEGPA
                     PAPGQFFMLAAAPDWAREGGRPYLPRAISHARFTRHPGSGQRLDFLIDEVGPGTSRLA
                     EMKPGDQCWVAGPFGGRFSLPRKLNPQAAGAILVGGGIGIAPMAVLRRELVDRGIPSR
                     SLLGFRDQAHSGGVEELFGCEETRLAFEDGSSGHKGYVTDLLLQLLEGDDSASAAVYS
                     CGPPAMLEAVRRICLEREVPVELALESPMACGYGACFGCAVPKADGGYLRLCIDGPVV
                     RGDEIATAMAGEPAVD"
     gene            1037..>1246
                     /locus_tag="PLE93_10735"
     CDS             1037..>1246
                     /locus_tag="PLE93_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87569.1"
                     /translation="MTESAKAAEPLPVMDLSTGICGIPLSGPVLNGSGTFDALAARRV
                     FGDALIEDRRHPDETQAFYARHLDRK"
BASE COUNT          176 a          436 c          435 g          199 t
ORIGIN      
        1 cgccgaccgg ttccggggcc cgggccgacg gttacgagat ccgctcggcc gcgatccggc
       61 acggcattcc ttcggtgacc acactgaccg gggcctcggc tgcggtccgg gcgatggccg
      121 cccgggccaa tggttccggg gaggtcaagt cacttcagga gcttcatgcc cgctgacccg
      181 gccgccacgc ctgaccgggt tcaggcgccg ttcggacggc gactctgcga agtcgctgaa
      241 acgaggccct cgggcggcta ccggatcttc acgctgatcg accccgaagg cccggccccg
      301 gccccgggcc agttcttcat gctggccgcc gcgcccgact gggcgaggga gggtggccgg
      361 ccctacctgc cgcgggcgat ctctcatgcc cggttcacac ggcaccccgg ctccgggcag
      421 cgacttgact tcctgatcga cgaggtcggc ccgggaactt cgaggctcgc ggagatgaag
      481 ccgggagatc aatgctgggt ggccgggccg ttcggtggcc gcttcagcct gcctcgcaag
      541 ctcaatccgc aggccgccgg ggcgatcctg gtcggcggcg ggatcggcat cgcgccgatg
      601 gcggtgctgc gccgtgaact cgtcgaccgc gggattccct cccgcagcct gctcggattc
      661 cgtgatcagg cgcattccgg cggggtggaa gaactcttcg gctgcgagga gacgaggctc
      721 gccttcgagg acggcagctc cggccacaag ggttacgtga ccgacctgct gctccagctg
      781 ctcgaaggtg acgattccgc ctcggccgcc gtctactcct gcggaccgcc ggcgatgctg
      841 gaggccgttc gccggatctg ccttgagcgg gaggtcccgg tcgagctcgc cctcgaatcg
      901 ccgatggcct gcgggtacgg agcctgcttc ggctgcgccg tgccaaaggc ggacggcggc
      961 tacctgagac tctgcatcga cggcccggtc gtccgtggcg acgagatcgc caccgcgatg
     1021 gcaggagagc cggccgttga ctgaatcggc aaaagcggca gaaccgctcc cggtcatgga
     1081 tctctccacc gggatctgcg gaattcccct cagcggtccc gtgctgaacg gttcggggac
     1141 cttcgatgcg ctggccgccc gccgggtctt cggcgacgcg ctgatcgagg accgtcggca
     1201 tccggacgaa acgcaggctt tctacgcccg gcacctcgac cgcaag
//
LOCUS       DAOQFI010000705         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_86067_length_1246_cov_2.269521, whole genome shotgun sequence.
ACCESSION   DAOQFI010000705 DAOQFI010000000
VERSION     DAOQFI010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86067_length_1246_cov_2.269521"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLE93_10740"
     CDS             complement(<1..679)
                     /locus_tag="PLE93_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNH87570.1"
                     /translation="MFVRSVRRGSDRRPSRGASARGAKAEKPAREPLRQHHLDLIGLG
                     CVLLGIYLVFVLYFGWNGGRLGSGISDGLKYLFGLGAYLAPVALFAAGAIAIFRPAIP
                     KTRPLRTGAALLAAGLLLAFAGGTFGIAGGRPERIAYFDPGWFPDHGGVIGESFYWAF
                     ASALQPFGAHLVAAFAILAGILLLTGTTVAAVLGRSGRRSGRPARHRPKRPAPLPGPQ
                     SSTTASPR"
     gene            745..>1246
                     /locus_tag="PLE93_10745"
     CDS             745..>1246
                     /locus_tag="PLE93_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH87571.1"
                     /translation="MVDEEQTGGRILVIEDDEEIVDVLRRTLRAEGYEVRATEDGGGG
                     LATASEFMPDLVVLDLGLPDMDGLEVCTRLREGSSELPILMLTARDGAGNRVTGLDSG
                     ADDYLEKPYNREELLARIRALLRRHPPRGSAMLTVGDLKLNPDSQEAMRGDREVELTK
                     REFELLE"
BASE COUNT          239 a          405 c          425 g          177 t
ORIGIN      
        1 ccctcgggct tgccgtcgtg gaggattgcg gtccgggcaa aggagcgggt cttttcggcc
       61 gatgtcgtgc cggccttcct gatcgccttc cgcttcgccc gagaaccgcc gcaaccgtgg
      121 ttccggtcag cagcagaatc ccggcgagga tcgcgaacgc cgccaccaga tgggcaccga
      181 agggctggag cgcactggcg aaagcccagt agaagctctc gccgatgacc ccgccgtggt
      241 ccgggaacca gcccgggtca aagtaggcga tccgctcggg acggccaccg gcgatcccga
      301 aggtaccccc ggcgaaggca agcagaaggc ccgcagcaag cagcgctgcc ccggtcctga
      361 gcggacgggt cttggggatg gccgggcgga agatcgcgat cgctccggcg gcaaagagcg
      421 cgaccggagc caggtaggcg ccgagaccga agaggtactt cagcccgtcg ctgattcccg
      481 aaccgagccg tccgccgttc cagccgaagt agagaacgaa aacgaggtag atcccgagca
      541 gcacacagcc cagtccgatc aggtccaggt gatgctggcg caggggctca cgagcgggct
      601 tctcggcttt ggctccgcgc gccgaggcgc cacgcgacgg gcgccggtct gaaccgcgcc
      661 ggactgaacg tacaaacatg gctttccttt caacgaaacg cgcgtttacc cttcctgtaa
      721 tcgacctcgc ttagaatccc ggccatggtg gatgaggagc agaccggcgg gcgcatactc
      781 gtcatcgagg acgacgagga aatcgtcgat gtcctcaggc gcacgctgag agccgaggga
      841 tacgaagtcc gggcgaccga ggacggtggc ggcggccttg cgaccgcttc cgaattcatg
      901 ccggacctgg tcgtgctgga ccttggcctg ccggacatgg acgggctcga ggtctgcacc
      961 cggctcaggg aaggcagcag cgagctgccg atcctgatgc tcaccgcccg cgacggcgcc
     1021 ggcaaccggg tcaccgggtt agacagcggc gccgacgact atctcgagaa gccctacaac
     1081 cgggaagagc tgctggcccg catccgggca ctgctgaggc gacatccgcc ccgtggctcg
     1141 gcgatgctca cggtcggcga cctgaagctg aacccggatt cccaggaagc gatgcgcggt
     1201 gaccgggaag tcgagctgac caaacgcgag ttcgaactgc tcgagt
//
LOCUS       DAOQFI010000706         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_86468_length_1243_cov_2.113636, whole genome shotgun sequence.
ACCESSION   DAOQFI010000706 DAOQFI010000000
VERSION     DAOQFI010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86468_length_1243_cov_2.113636"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLE93_10750"
     CDS             complement(<1..338)
                     /locus_tag="PLE93_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HNH87572.1"
                     /translation="MKLIVNVDGGARGNPGPAAIGIVVRDGEGNVLQDLGETIGEATN
                     NVAEYRALIKGIGLARELGATELQIHGDSELVVKQMLGQYKVKHPDMKPLHAEAKAAL
                     AKISATGGTN"
     gene            complement(393..>1243)
                     /locus_tag="PLE93_10755"
     CDS             complement(393..>1243)
                     /locus_tag="PLE93_10755"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HNH87573.1"
                     /translation="LLAAGTAPRTLNAYEKDLGQLRAWADGSGLGVDKVTHKDLRRYA
                     ASLSAAGRAPATVARKLAAIRGFYGFLLRTGRVGQNPADLVSAPRRKKKLPTVLTMEQ
                     MSSMLDAVPVETPLDIRDRAMFELTYSCGLRSEEVLTLTLDSIDFDDEVIRVLGKGSK
                     HRLVPVGEPARQAIELYLARARPKLATDPAERTLFLSRNGRPLSASDLSRRLAVRMRD
                     AAAAGGVSPHALRHSFATHLLEGGADLRSIQELLGHASISTTQVYTHLDAARLRDAYT
                     GSHPRA"
BASE COUNT          187 a          394 c          435 g          227 t
ORIGIN      
        1 tagtttgtgc cgccagtggc gctgatcttg gcgagcgcgg ccttggcttc ggcgtggagc
       61 ggcttcatgt ccggatgctt gaccttgtac tgaccgagca tctgcttgac gaccagttcc
      121 gaatcaccgt ggatctgaag ttcggtcgcg ccgagttccc gggccaggcc gatgcccttg
      181 atcagggccc tgtactcggc cacgttgttt gtggcttcgc cgatcgtctc gccgagatcc
      241 tggagcacgt tgccctcacc gtcccgaacg actattccga tcgcagccgg gcccggattg
      301 ccccgggcgc cgccgtccac gttgacgatc agcttcatgg cagggaatct attgcctgcg
      361 ccgggcggtc gactcccggc tgaagcgccc cttcaggcgc ggggatggct gccggtgtac
      421 gcgtcccgca ggcgggccgc gtcgaggtgg gtgtagacct gtgtggtcga gatcgaggcg
      481 tggccgagca gttcctggat cgagcgcagg tccgccccgc cttcgagcag gtgggtggcg
      541 aaggaatgcc tgagggcatg gggcgaaacc ccgccggccg cggcggcatc ccgcatgcgg
      601 accgccaggc gacgggaaag gtcggaggcc gaaaggggtc ggccgtttcg cgaaaggaac
      661 agggtcctct cggccgggtc ggtggccagt ttcggccggg ccctggcgag gtagagctcg
      721 atcgcctgcc gggccggctc gccgaccggg accagccggt gcttggaccc cttgccgagc
      781 acccggatca cttcgtcgtc gaaatcgatc gagtcgaggg tgagggtcag tacctcttcg
      841 cttcgcagac cgcaggagta ggtgagttcg aacatcgccc ggtccctgat gtcaagcggg
      901 gtttcgaccg gtaccgcgtc cagcatcgag gacatctgtt ccatcgtcag aacggtcggc
      961 agcttcttct tccggcgggg tgcggaaacc aggtcggccg ggttctgacc aacccttccg
     1021 gtcctcagaa ggaagccgta aaagccacgg atcgcggcca gtttgcgagc caccgtggcc
     1081 ggggcgcggc ccgccgccga aagcgaggcg gcgtagcggc gaagatcctt gtgggtgacc
     1141 ttgtctaccc ccaggcctga cccatcggcc caggcccgca gctggccgag gtccttttcg
     1201 taggcgttca gggttcgcgg tgccgtacct gccgcgagaa gat
//
LOCUS       DAOQFI010000707         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_86846_length_1240_cov_2.517300, whole genome shotgun sequence.
ACCESSION   DAOQFI010000707 DAOQFI010000000
VERSION     DAOQFI010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86846_length_1240_cov_2.517300"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..529)
                     /locus_tag="PLE93_10760"
     CDS             complement(167..529)
                     /locus_tag="PLE93_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87574.1"
                     /translation="MNQDDQQNSRNTATARFAGWLAVGVLFMVGCIAPISFFDWIPAA
                     LLAGFLIWRGVRSPRYFFAFAAGIGITLVGLGIVNGTYRIMLLYGSVVIAGGIVFYAA
                     FCPRTSHAEPPGGKGSQS"
     gene            complement(526..>1240)
                     /gene="murI"
                     /locus_tag="PLE93_10765"
     CDS             complement(526..>1240)
                     /gene="murI"
                     /locus_tag="PLE93_10765"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNH87575.1"
                     /translation="YLGDSGHFPYGTKSAEELRDRAQVIATGLLERGVKMLVIACNSA
                     TAAAGDEIRQLGAEAGVPVVTVVGPEAEIAATISGNGKVGVLATPATVASGAYRRALD
                     SLGRPLTVTEVAAPDLAPIIQQGPTFDQGVLDTVRSYCDPLREAGVDTLILGCTHYPL
                     VAPMLQRMLGRDVSLVTAGHAIAAAIQRELASRDELTTDRIEGDYRFLVTGDVDSFVE
                     AGTRFLQLPLAGRAEKVEL"
BASE COUNT          213 a          408 c          411 g          208 t
ORIGIN      
        1 tttcctgctg gaggacgtcc tggatcattc gaagcgccga ggcacgcttg cggtcgccgt
       61 ccttgagggc ggcctgtgcg tcttcgcgaa tctggtcggc ggctgacatg ggactagcgt
      121 acctgcccgg gcagccgaac ccgctcaccg cctgtgaaga agcggttcag ctctggcttc
      181 ccttgccgcc gggcggctcc gcatgcgaag tacgggggca aaaggcggcg tagaagacga
      241 ttcccccggc gatcacgacc gacccgtaga gcagcatgat gcggtaggtc ccgttgacga
      301 tcccgaggcc gaccagggtg atcccgatcc cggccgcgaa ggcaaagaag tatcggggcg
      361 agcgaactcc ccgccagatc aggaaaccgg ccagcagcgc cgccggaatc cagtcgaaga
      421 agctgatcgg ggcgatgcag ccaaccatga agagaacccc gaccgccagc catccggcga
      481 acctggcggt tgccgtgttg cggctgtttt gctgatcgtc ctgattcaaa gttccacctt
      541 ctcggcccgt ccggccagcg gcagttgcag gaatcgggtt ccggcttcga cgaaggaatc
      601 aacgtcaccg gtgaccagga aacggtagtc gccttcgatc cggtcggtgg tcaactcgtc
      661 gcggcttgcc agttcccgct ggattgccgc ggcgatcgcg tggccggccg tgaccaggct
      721 gacgtcccgg ccgagcatgc gctgcagcat cggagcaacc agcgggtagt gggtacagcc
      781 gaggatcaag gtgtcgacac ccgcttcacg gagcgggtca cagtagctgc ggactgtgtc
      841 gagaaccccc tggtcaaagg tcgggccttg ctggatgatc ggtgccagat caggtgccgc
      901 gacctcggtc acggtcagcg gtcgacccag ggaatcgagt gcccgccggt aggcgccact
      961 ggcgacggtc gccggcgtcg cgagcacgcc cacttttccg ttgccgctga tcgtggcggc
     1021 gatctccgct tccggaccga ccacggtcac caccggcacc ccggcttcag ccccgagctg
     1081 acgaatctcg tcgccggccg cggcggtggc cgagttgcag gcgatgacca gcatcttcac
     1141 cccgcgttcc agcagcccgg tggcgatcac ctgggcgcga tcgcgcagct cttcggcgct
     1201 cttcgtcccg taagggaagt gaccgctgtc acctaggtag
//
LOCUS       DAOQFI010000708         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_86952_length_1239_cov_3.226351, whole genome shotgun sequence.
ACCESSION   DAOQFI010000708 DAOQFI010000000
VERSION     DAOQFI010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86952_length_1239_cov_3.226351"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLE93_10770"
     CDS             complement(<1..455)
                     /locus_tag="PLE93_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87576.1"
                     /translation="MSQAMEANQPAAEPAAGTDPVVFPPSPPGVDPPEDPGKYPFATW
                     SPWVAIGGAVAALIFGVMLALPVDVVAGGENFDDYGWQVKALFQLCTVVGFLAVPFLF
                     AKELPGGVNWREAAGRLGFQRFRIGTAAKWIGLGFLAYIAFAIVYAVVFG"
     gene            560..>1239
                     /locus_tag="PLE93_10775"
     CDS             560..>1239
                     /locus_tag="PLE93_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNH87577.1"
                     /translation="MTRRQGRVRRHRGSPGKVFVAVAAVLGGLLLTAIAAAGGWVLSI
                     AADAPNPDNLKEINKGQNSVIYAGDGSKLGLINSTEIRTPIPLSKIPQSIQDATVAIE
                     DERFYQHDGVDVQGGIRALFKNLEAGEVNEGASTITMQLMRNIYISNPQRDIERKIKE
                     AKMALDYEKDHTKKQILEKYLNTAPYGTNGGRSAIGVNAAAKVYFSVKPENMSLPQAA
                     LLAGLPQAP"
BASE COUNT          252 a          416 c          387 g          184 t
ORIGIN      
        1 ccgaaaacca cggcatagac gatcgcgaag gcgatgtagg cgaggaatcc gagcccgatc
       61 cacttggccg ccgtgccgat gcgaaagcgc tggaacccga gccggccggc cgcttcccgc
      121 cagttgaccc cgcccggaag ttccttcgcg aagaggaagg gaaccgccag gaaaccgacc
      181 accgtgcaga gctggaacag cgccttgacc tgccagccgt agtcatcgaa attctcgccg
      241 ccggccacga cgtcgaccgg cagggccagc atcacaccga agatcagggc cgcaacggct
      301 ccgccgatcg cgacccacgg gctccaggtc gcgaaggggt acttcccggg atcttcgggc
      361 gggtcgaccc cgggtgggga agggggaaag acgaccgggt cggtcccggc tgccggctcc
      421 gctgcgggct ggttggcttc catggcttgg ctcattctca caattcgggg ccgcagtcgt
      481 ccgcaaccca tcctgcaccg gcgtcgcctg gaaccgccct ttagaagttc ctgaggtgct
      541 tccagctacc ctttagggga tgaccaggcg ccagggaagg gtgcgccgtc accgtggttc
      601 acccggcaag gtttttgtcg ccgtggccgc agtgctcggc ggattgctgc taaccgcgat
      661 tgcggctgcc ggcggctggg ttctttcgat cgccgccgac gccccgaatc cggacaatct
      721 caaggagatc aacaagggcc agaactcggt catctacgcc ggtgacggca gcaagctcgg
      781 tctgatcaac tcgaccgaaa tccgcacccc gatcccgctt tcgaagatcc cgcagtcgat
      841 ccaggacgca acggtcgcga tcgaggacga gcgcttctac cagcacgacg gcgttgacgt
      901 ccagggcggc attcgggccc tgttcaagaa cctcgaggcc ggtgaggtga acgaaggcgc
      961 ctcgacgatc accatgcagc tgatgcggaa catctacatc tcgaacccgc aacgggacat
     1021 cgagcggaag atcaaggaag ccaagatggc cctcgattac gagaaggacc acaccaagaa
     1081 gcagatcctc gagaagtacc tgaacaccgc cccctacggc accaacggcg gacgcagcgc
     1141 gatcggcgtg aacgcggccg ccaaggtcta cttctcggtc aagccggaga acatgagcct
     1201 gccccaggcc gccctgctgg ccggtctgcc gcaggcccc
//
LOCUS       DAOQFI010000709         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_87087_length_1238_cov_2.812342, whole genome shotgun sequence.
ACCESSION   DAOQFI010000709 DAOQFI010000000
VERSION     DAOQFI010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87087_length_1238_cov_2.812342"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLE93_10780"
     CDS             complement(<1..191)
                     /locus_tag="PLE93_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87578.1"
                     /translation="MPLLEPDVMKISPEASEPALDRAPDWVAGGTPEPLRSRLTALLG
                     EEKLLGRATDLIRYASDASP"
     gene            complement(216..>1238)
                     /locus_tag="PLE93_10785"
     CDS             complement(216..>1238)
                     /locus_tag="PLE93_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH87579.1"
                     /translation="ADLAARLKQAENPVFVAGPDIDAAGGWDEAIALAEGQNLPVWAS
                     PATGGGRLGFPESHPHWRGVLPPGIAAIAEVLKEHDLILVVGSSVFPYYPYMPGDFLG
                     ENSKLVQVTSDPDEAARAPMGEAIVADVKLTLAALNGELDGTERPAIEPWPGPTRMEE
                     TDPLNPSVVMNTLKDVMPEDAVIVLEAPTSTMALRNQLRISKPNSYFFGAGGGLGFGL
                     SSAVGIGYAEDRPVICVLGEGSVQYAVTAFWTAAAYDIPVTFLILRNEEYGILKWFAE
                     VEQVSDAPGLDLPALETAKIAEGYGIESTTAKSAEEVAAALKEAIADSRPRLVEVPVE
                     PGMSLF"
BASE COUNT          189 a          418 c          421 g          210 t
ORIGIN      
        1 gggctggcgt ccgaggcgta gcggatcagg tcggtcgccc ggccgagcag cttctcctcg
       61 ccgagcagcg cggtgagccg ggagcggagc ggttcgggtg tgccgcccgc aacccagtcg
      121 ggagcccggt cgagtgccgg ctcgctcgcc tccgggctga tcttcatgac atccggttcg
      181 agcagtggca tctcagactc cctgacagtt ggtttctaga acagcgacat tcccggctcg
      241 accggcactt cgaccagccg cggcctggaa tccgcgatcg cttccttcag cgccgcggct
      301 acttcctctg cactcttcgc cgtcgtgctc tcgattccgt acccctcggc gatcttcgcc
      361 gtctccaggg ccggcaggtc gagcccgggg gcatcggaaa cctgctcgac ctcggcgaac
      421 cacttgagga tcccgtactc ctcgttgcga aggatcagga aggtgaccgg gatgtcatag
      481 gcggcggcgg tccagaaggc agtcaccgcg tactgcacag aaccttcgcc gagcacgcag
      541 atcaccgggc gatcctcggc gtaaccgatg ccgaccgcgg aggaaaggcc gaagccgagt
      601 ccgccgccgg ccccgaagaa gtaactgttc ggcttcgaga tccggagctg gttgcggagc
      661 gccatggtcg aggtcggagc ttcgagcacg atcaccgcgt cttccggcat cacgtccttc
      721 agcgtgttca tcacgaccga cgggttgagc ggatcggtct cttccatccg ggtcggcccc
      781 ggccacggtt cgattgccgg ccgctcggtg ccgtcgagct cgccgttcag ggcggccagg
      841 gtcagtttca cgtcggcaac gatcgcctcg cccatcgggg cgcgggccgc ctcgtccggg
      901 tcggaggtga cctggaccag cttcgagttc tcgccgagga agtcgccggg catgtacggg
      961 tagtagggaa agacggacga gccgaccacc aggatcaggt catgttcctt cagcacctcg
     1021 gcgatcgcgg caatgccggg cggcagcacc ccgcgccaat gcgggtgact ttccgggaag
     1081 ccaagccggc cgccaccggt tgcgggggag gcccagaccg gcaggttctg gccttcggcc
     1141 agggcgatcg cttcgtccca gccgccggcc gcgtcaatgt cgggtccggc cacgaagacc
     1201 gggttctctg cctgcttgag gcgggcggcg aggtcggc
//
LOCUS       DAOQFI010000710         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_87328_length_1236_cov_3.288738, whole genome shotgun sequence.
ACCESSION   DAOQFI010000710 DAOQFI010000000
VERSION     DAOQFI010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87328_length_1236_cov_3.288738"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1006
                     /locus_tag="PLE93_10790"
     CDS             98..1006
                     /locus_tag="PLE93_10790"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNH87580.1"
                     /translation="MKLETMVCETGEGQGYIVLNRPERGNGLTPELIRELAETVELLD
                     LDPEVRVIVLSGNGSGFCGGYDLVASAEKMLEGEFGSEDAPAGSPLDPMVQAANHDPS
                     KTWDPMVDYAGMSRNVRAFMSLFHCTKPVICKVHGFCVAGGTDLALCSDLLVIADDAK
                     IGYPPARVWGSPTTSLWAYRLGAQRAKRLLFTGDSLTGREAAEAGLAIESHPREKLDE
                     AVDALAARIAMVPLNQLQMMKLLVNQSLYAQGLHQTQLIGTVFDGITRHTPEGFAFQQ
                     RAAEAGFKQAVRERDEPFGDFGLKPD"
     gene            complement(1023..>1236)
                     /locus_tag="PLE93_10795"
     CDS             complement(1023..>1236)
                     /locus_tag="PLE93_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87581.1"
                     /translation="DSGAIAWPYEALGVAYCAFGLGLIFFGVQRGKLSAEHHVEKPTV
                     PAGGAFLAAGGLILGIATILVVALAR"
BASE COUNT          238 a          405 c          405 g          188 t
ORIGIN      
        1 ctgacgacac cgccgactgc gaccagggag agctccacgg cagcgaagcc taaccgcgaa
       61 actaacctcc ggctcaaggc ccggttagca tcgcctcatg aagctggaga cgatggtctg
      121 cgagacaggg gagggtcagg gatacatcgt cctgaaccgt ccggagcgcg gcaatggact
      181 gacgccagag ctgatccgcg aactggcgga aacggtggag cttctcgacc tcgaccccga
      241 ggtccgggta atcgttctct ccggcaacgg cagcgggttc tgcgggggat acgacctcgt
      301 ggcaagcgcc gaaaagatgc tggaaggtga attcggctcc gaggacgcgc ccgccggctc
      361 accgctcgat ccgatggtgc aggcggccaa ccatgacccg tcgaagacct gggatccaat
      421 ggtcgactac gccggcatga gccggaacgt gcgggccttc atgtcgctgt ttcactgcac
      481 caaaccggtg atctgcaagg ttcacggctt ctgcgtcgcc ggtggcaccg acctggccct
      541 gtgcagcgac cttctggtga tcgccgacga cgccaagatc ggctacccgc cggcccgggt
      601 ctggggttct ccgaccactt cgctctgggc ttacaggctc ggggcgcaac gggccaagcg
      661 gctgctcttc accggtgaca gccttaccgg ccgggaagcg gcagaagccg gtctggcaat
      721 cgagtcgcac ccgcgtgaga agctcgacga ggcagtcgac gcgctggccg cccggatcgc
      781 aatggttccc ctgaaccagc tgcagatgat gaagctcctc gtcaaccagt cgctctacgc
      841 ccagggcctc caccagaccc agttgatcgg cacggtcttt gacggcatca cccgccacac
      901 cccggagggc tttgccttcc agcaaagggc ggccgaagcc ggcttcaaac aggcggttcg
      961 cgaacgcgac gaaccattcg gggacttcgg cctgaaaccg gactgagccg ggcgggtccg
     1021 gctcagcggg cgagagcgac cacgaggatc gttgcgatcc cgagaatgag tcctcccgcg
     1081 gcgaggaacg ctccgccagc cggcacggtc ggcttctcga catggtgttc ggccgagagc
     1141 ttgccgcgct ggacgccgaa gaagatcagg cccaggccga aggcgcagta ggcaacgccc
     1201 agggcttcat agggccaggc gatcgctccg gaatcg
//
LOCUS       DAOQFI010000711         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_88037_length_1231_cov_2.738095, whole genome shotgun sequence.
ACCESSION   DAOQFI010000711 DAOQFI010000000
VERSION     DAOQFI010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88037_length_1231_cov_2.738095"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..605)
                     /locus_tag="PLE93_10800"
     CDS             complement(54..605)
                     /locus_tag="PLE93_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87582.1"
                     /translation="MTVFEPTLSSEKLTEATGKNWDEWLELLDQAGAIKRTHTEIARW
                     LTDEQGVPGWWAQSITVGYERARGLREPGQRADGWSVTASKTIEVPVEELFEAFERDT
                     IREKWLPDAELHLRTATAPVSARYDWGDGTTRLIVGFESLGPNRSKVALEHEKLPDAQ
                     VADRMKDWWRERVAELKELLEKG"
     gene            672..956
                     /locus_tag="PLE93_10805"
     CDS             672..956
                     /locus_tag="PLE93_10805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH87583.1"
                     /translation="MPETSEDNVEVTDQPDASRYEIREAGELAGFAEYRLHGDVITFT
                     HTEVDPERGGRGLGSRLIEFAVTDARSRELSIIPRCPFVRDWIAEHPEED"
     gene            953..>1231
                     /locus_tag="PLE93_10810"
     CDS             953..>1231
                     /locus_tag="PLE93_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87584.1"
                     /translation="MTATRQQAEAAADQVPGSKLSARLYEPFLWLGEKTGFRRWRQKV
                     VSGVSGSVLELGAGTGLNLVHYPAGLDRLVLVEPDFHKAGILATKPAPA"
BASE COUNT          199 a          436 c          384 g          212 t
ORIGIN      
        1 gcttgcgatc tacctgatga tcgcccaacc cggagcctga acccacgggc ggatcagccc
       61 ttttcgagaa gttccttgag ctcggcgacg cgttcgcgcc accagtcctt catccggtcg
      121 gcgacctggg cgtcgggcag cttctcgtgc tccagggcga ccttgctccg gttcgggccg
      181 aggctctcga acccgacgat cagccgggtg gttccgtcac cccagtcgta gcgggccgaa
      241 accggggcgg tcgccgtgcg caggtgaagc tcggcgtcgg gcagccactt ctcccggatc
      301 gtgtcccgct cgaaggcttc gaacagctct tcgaccggaa cctcgatcgt ctttgaggcc
      361 gttaccgacc agccgtcggc tcgctggccc ggctccctca ggccgcgggc cctttcgtat
      421 ccgaccgtga tcgactgggc ccaccagccg gggacgccct gctcatcggt cagccagcgg
      481 gcgatctcgg tgtgggtccg cttgatcgcg ccggcctggt cgagcaactc gagccactcg
      541 tcccagttct tgccggtcgc ctcggtgagc ttttcgctcg aaagcgtcgg ttcgaagacg
      601 gtcatgcctc aagatcattt aaccaccagc cgcccggtcg aaacagaaat ccacctctct
      661 aacattggac catgccggaa acctcagaag acaacgtcga agtcaccgac cagccagacg
      721 ccagccgata tgaaatccgg gaggccggcg agctcgccgg cttcgccgaa tacaggctcc
      781 acggggacgt gatcaccttc acccacaccg aggtagaccc ggagcgcggc ggccggggcc
      841 tcggatcccg gctgatcgag ttcgcggtca ccgacgcccg ctcgcgcgag ctctcgatca
      901 tccccaggtg ccccttcgtc cgggactgga tcgccgaaca cccggaggaa gattgaccgc
      961 gaccaggcag caggccgaag cggcggccga ccaggttccc ggcagcaagc tctcggcccg
     1021 tctctacgaa ccgttcctct ggctcggtga gaagaccggg tttcgccgct ggcgccagaa
     1081 ggtcgtttcc ggggtttccg gatcggtgct cgagctcggg gccgggaccg ggctgaacct
     1141 ggttcattac ccggccggtc tcgaccggct ggtcctggtc gagccggatt tccacaaggc
     1201 cgggatcctg gccaccaagc ccgcgccggc c
//
LOCUS       DAOQFI010000712         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_88465_length_1228_cov_2.637681, whole genome shotgun sequence.
ACCESSION   DAOQFI010000712 DAOQFI010000000
VERSION     DAOQFI010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88465_length_1228_cov_2.637681"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1228
                     /locus_tag="PLE93_10815"
     CDS             <1..>1228
                     /locus_tag="PLE93_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87585.1"
                     /translation="GEVWQRTNSFNLEWTEPDQGPASPIVLSYFRLTGPDGYAGGPWY
                     RTDPGKISGITLPGPGEYKARVWLADQAGNSDEAHAAEATLRLDNVPPTGYFVDPPED
                     DPALIRVSVADRFSGVQGGSIAWRPADGGDWHPISSDYLGDRQMLEARFPDDLPRGSW
                     VLRAAIADGAGNLTVTDRRANGSQMIVRTPLLDETQVSASLGSSRASRGRGTVEIGYG
                     ERAHLEGRLTGVESGGLGGATLTVTETPFPGSRAKVTSRQVRTDARGYFDLWLARGPG
                     RRVVVGFAGTRRLQGSDSGVLELRVRGRLGFKARPKRLRTGRRVYFTGRVAARGAWQP
                     TRGNLIQIQYFEESARRWRPIALTRTNRYGRYRSSYRFRYITGLARIRLRALLVPSSR
                     FPYSGAASKPVVIRVRG"
BASE COUNT          182 a          440 c          433 g          173 t
ORIGIN      
        1 ggcgaagtct ggcagcggac caacagcttc aacctcgagt ggaccgaacc cgatcagggg
       61 ccggcctccc cgatcgtcct cagttacttc cgcctgaccg gtcccgacgg gtatgccggc
      121 ggcccctggt accggaccga cccgggaaag atcagcggaa tcaccctgcc cggaccgggg
      181 gaatacaaag cccgggtctg gctggccgac caggccggca acagcgacga ggcccacgcg
      241 gccgaagcga cgctccgcct cgacaacgtt cccccgaccg gttacttcgt cgaccccccg
      301 gaagatgacc cggccctgat ccgggtttcg gtcgccgacc gcttctccgg ggtgcaaggc
      361 ggaagcatcg cctggcggcc ggccgacggc ggcgactggc acccgatctc atccgactac
      421 ctgggcgacc ggcagatgct cgaagcccgg ttccccgatg acctgccgcg aggcagctgg
      481 gtgttgcggg cggcgatcgc cgacggggcc ggcaacctga ccgtgaccga ccgccgggcc
      541 aacggctcgc agatgatcgt tcgcacgccg ctgctggacg agacgcaggt ttccgcctcg
      601 ctgggttcga gccgggccag ccggggccgg ggcacggtcg agatcggtta cggggagagg
      661 gcccatctcg aaggccggct gaccggggtc gagagcggtg gcctcggcgg ggcgacgctg
      721 accgtcaccg agactccctt cccgggctcc cgggccaagg tgacgagccg gcaggtccgg
      781 accgatgccc gcggctactt cgacctctgg ctggcccgcg gaccgggtcg tcgggttgtc
      841 gtcggcttcg ccggaaccag gcggctccag ggatcggatt cgggcgtgct cgaactgagg
      901 gtgcggggcc ggctcggctt caaggcgagg ccgaagcggc tgaggaccgg ccggcgggtc
      961 tacttcaccg gccgggttgc cgcccgcgga gcctggcagc cgacccgcgg caacctgatc
     1021 cagatccagt acttcgagga gtcggcccgc cgctggcggc caatcgcgct gacccggacc
     1081 aaccgttatg gccggtaccg gagcagctac cgcttccgct acatcacggg cctcgcccgg
     1141 atcaggctgc gggcgctcct cgtcccctcg tcccgcttcc cctacagcgg ggcggcctcg
     1201 aaacctgtgg tcatacgggt tcggggct
//
LOCUS       DAOQFI010000713         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_88686_length_1226_cov_3.761742, whole genome shotgun sequence.
ACCESSION   DAOQFI010000713 DAOQFI010000000
VERSION     DAOQFI010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88686_length_1226_cov_3.761742"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..562)
                     /locus_tag="PLE93_10820"
     CDS             complement(2..562)
                     /locus_tag="PLE93_10820"
                     /inference="COORDINATES: protein motif:HMM:NF020028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87586.1"
                     /translation="MSDSDTALAEVTWNLDDLLEGDHADPEAAVTALLDEAEKASEKF
                     AAEYEGKVSELDGPGLIVAMTKLADISDRAGRALNYAHLRFAADTADPANGALLQMGS
                     ERATRIQTQLLFFDLEWAALDDEVAEALLATPGLEFCEHHLRNERKYRDHLLSAPEEK
                     IVTELSVTGAGAWSRLFDELTSARWS"
     gene            complement(559..900)
                     /locus_tag="PLE93_10825"
     CDS             complement(559..900)
                     /locus_tag="PLE93_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87587.1"
                     /translation="MSTSEDLSPQAPGGAREIRFLNIIAGVAILDFLLLIPLVWASRW
                     FADKHDIVSVLGPIHGFFFVVLIGLCAYGSLEKWWGWWFPILTLVTGGAIGSLIGDLV
                     VRRQLREKAAA"
     gene            complement(1007..>1226)
                     /locus_tag="PLE93_10830"
     CDS             complement(1007..>1226)
                     /locus_tag="PLE93_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87588.1"
                     /translation="LIALGVLLITGMMMASHFQLWDYGPFQVKMTLVIATIVAVLIHT
                     LRGNSHVLMGITFLLTLATVYAGVNLTH"
BASE COUNT          217 a          379 c          422 g          208 t
ORIGIN      
        1 gtcagctcca cctcgccgag gtgagctcgt cgaagagccg ggaccaggct ccggcgcccg
       61 tgaccgagag ttcggtcacg atcttttctt ccggggcgct cagcagatgg tcgcggtact
      121 tgcgctcgtt tctcaggtgg tgctcgcaga actcgagacc cggtgtggcc agcagcgctt
      181 cggccacctc gtcatcgagg gcggcccact cgaggtcgaa gaagagcagc tgggtctgga
      241 tccgggtcgc ccgctccgag cccatctgga gcagggcccc gttggccgga tcggcggtgt
      301 cggccgcgaa gcgcaggtgg gcgtagttga gcgcccggcc ggcccggtcg gagatgtcgg
      361 ccagtttggt catggcgacg atcaggccgg gaccgtccag ctcggagacc ttgccttcgt
      421 actcggctgc gaacttctcc gaagccttct ccgcttcgtc gagcagcgcc gtgaccgctg
      481 cctccgggtc ggcgtggtcg ccttcgagca ggtcgtcgag gttccaggtg acttcggcca
      541 gggcggtgtc ggaatcgctc atgctgccgc cttctccctg agctggcgcc gaacgaccag
      601 gtcaccgatc agggagccga tcgctccgcc ggtgaccagg gtcaggatcg ggaaccacca
      661 gccccaccac ttctcgagtg acccgtaggc gcagagcccg atcaggacca cgaagaagaa
      721 gccgtggatc ggcccgagca ccgagacgat gtcgtgcttg tcggcgaacc agcgcgaggc
      781 ccagaccagc gggatcagca gcaggaagtc gaggatcgcg accccggcga tgatgttgag
      841 gaagcggatt tcgcgggcac cgccaggtgc ctgaggggaa aggtcttcag aggtgctcat
      901 ggacccgtga agctatcaac ccgtcgatcc cgcccggttg tcaccgagaa ggcaaaagtg
      961 gccacatagc gctcactttg gccttctcga tccggtccgg gggaaatcag tgggtcaggt
     1021 tcactccggc gtagaccgtc gcgagggtca gcaggaaggt gattcccatc agcacgtggc
     1081 tgttaccgcg cagggtgtgg atcaggaccg cgacgatggt ggcgatgacc agggtcatct
     1141 tgacctggaa aggcccgtag tcccagagct ggaaatggga ggccatcatc atgccggtga
     1201 tcagcagcac gccgagcgcg atcagg
//
LOCUS       DAOQFI010000714         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_88903_length_1225_cov_2.276923, whole genome shotgun sequence.
ACCESSION   DAOQFI010000714 DAOQFI010000000
VERSION     DAOQFI010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88903_length_1225_cov_2.276923"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1225)
                     /gene="glmM"
                     /locus_tag="PLE93_10835"
     CDS             complement(<1..>1225)
                     /gene="glmM"
                     /locus_tag="PLE93_10835"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNH87589.1"
                     /translation="VGEELNAELALAIGRAGTESLRRARDIERPRVLIIRDTRESGPM
                     LESALAAGITEAGGDVYLGGVLPTPAAALVSGHHGFDLAAVVSASHNPFHDNGIKLFG
                     GDGGKLDDATEAGVEALIHEPPPPAGDPGRIRELIGADQDYLRALESFFRLDLGGLKV
                     VLDCANGATYRVAGEIFTRLGADVELIAIEPDGRNINDGCGSTDLGPLSAKVAASDAA
                     LGFAFDGDGDRVLAVDREGNPFDGDEMISLIAAERAATGSLGGGVAVTVMSNYGFHQA
                     MKEAGVEVEVTNVGDRYVLEALREKNWVLGGEQSGHIISTDYAPTGDGIAAALMLLEA
                     LNGRDLAEARVMEKLPQVLVNVKVSDREAIARADTVWTEVDRLNSELEGQGRVLLRAS
                     GTEPLVRVMAEAPTEA"
BASE COUNT          177 a          440 c          412 g          196 t
ORIGIN      
        1 ggcctcggta ggagcttccg ccatgacccg caccagcggc tcggtaccgg aggcccggag
       61 cagcacccgc ccctggcctt cgagttcgga attgagccgg tcgacctcgg tccagaccgt
      121 gtcggccctg gcgatcgcct cccggtcgct caccttgacg ttgaccagca cctgcggcag
      181 tttctccatc acccgggcct cggcgaggtc gcgtccgttc agggcctcaa gcagcatcag
      241 agcggctgcg atgccgtccc cggtcggggc gtaatcggtc gagatgatgt ggcccgactg
      301 ctcgccgccg agcacccagt tcttctcgcg cagggcctcg agcacgtagc ggtccccgac
      361 gttggtcacc tcgacctcca ccccggcctc cttcatcgcc tggtggaagc cgtagttgct
      421 catcacggtc accgccaccc cgccgccgag gctcccggtg gcagcgcgct cggccgcgat
      481 cagactgatc atctcgtccc cgtcaaaggg gttgccttca cggtcaacgg cgagcacgcg
      541 gtcgccgtcg ccgtcaaagg cgaagcccag cgcggcatcg gaagcagcga cctttgccga
      601 cagcggtccg aggtcggttg atccgcagcc gtcgttgatg ttgcggccgt cgggctcgat
      661 cgcgatcagc tcgacatccg ccccgagccg ggtgaagatc tccccggcga cccggtaagt
      721 ggcaccgttg gcgcagtcga gcactacctt gaggccgccc agatcgagcc ggaagaagct
      781 ctccagggcc cgcaggtaat cctggtcggc cccgatcagt tcgcggatcc ggccggggtc
      841 gccggccggc ggcgggggct cgtggatcag cgcctccacg ccggcttcgg tcgcgtcatc
      901 gagcttgccg ccgtctccgc cgaagagctt gatcccgttg tcgtggaagg ggttgtgcga
      961 agccgaaacc accgcggcaa ggtcaaagcc gtgatggccg gagaccagcg cggcagccgg
     1021 ggtcggcagg acgccgccga ggtagacgtc ccctcccgct tcggtgatgc cggcggccag
     1081 cgccgattcg agcatcggcc ccgactcgcg ggtatcgcgg atgatcagga cccgcgggcg
     1141 ttcgatgtcg cgggcacggc gcagcgattc ggtgccggcc cggccgatcg cgagggccag
     1201 ctccgcgttc agttcctcgc cgacg
//
LOCUS       DAOQFI010000715         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_89491_length_1221_cov_2.235849, whole genome shotgun sequence.
ACCESSION   DAOQFI010000715 DAOQFI010000000
VERSION     DAOQFI010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89491_length_1221_cov_2.235849"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..335
                     /locus_tag="PLE93_10840"
     tRNA            264..335
                     /locus_tag="PLE93_10840"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Glu,seq:ttc)
     gene            414..1208
                     /locus_tag="PLE93_10845"
     CDS             414..1208
                     /locus_tag="PLE93_10845"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH87590.1"
                     /translation="MIPEISLGPITLQTFGLAFAAAFIVSGWIVHLRLEEIGKPPDWA
                     YEMVFAALIGGLIGARAYFIFDNYADVKDDLLGNIFSGSGLTWYGGAIGGAIGVILWA
                     WWRKFLNLWLLDTAAPALAAGYAVGRIGCQLSGDGDYGKPWDGPWAMSYPDGTVPTPD
                     GVTVHPTPIYETLTMGLGAYILWRLRDRFRLGILFAIYLIYAGTERLLVEFLRRNTDV
                     FLGLTQAQLESVAMILGGIIWILVVRRRYGSLARPADQMSAADVPA"
BASE COUNT          182 a          443 c          379 g          217 t
ORIGIN      
        1 ccgcctccac gcccagaagg ccctgctcga gtacctgctc gccccctgac acccgctcgc
       61 cccctgacac ccgctcaccc ctgacacccg ctcacccctg acacccgctc gcccctgaca
      121 cctgctcgcc ccctggcctc gggatctgcc cgaatcgaga gggctaaatg gagcgctata
      181 tggccacttt tgccttctcg cgcggccggg gaaccggtcg gcaagccgcc actcgcgttc
      241 aactgagaga atgtccgccg cgcggccccg tggtgtaacg gttagcacgc caggttttca
      301 acctggaagc gcgggttcga ctcccgccgg ggctactgct cgccctttgc cagggccggc
      361 gaagcggtcc ggccggaatc gcaggcctga cccgcaccgg tagggtccgg ggcgtgattc
      421 cggagatctc gctcggcccg ataactctgc agaccttcgg tctcgctttc gcggccgcct
      481 tcatcgtttc cggatggatc gtccacctgc gactggagga gatcggcaaa ccgccggact
      541 gggcttacga gatggtcttc gccgcgctga tcggcggttt gatcggggcc cgggcctact
      601 tcatcttcga caactacgcc gatgtcaagg acgacctgct cggcaacatc ttcagtggtt
      661 ccggcctgac ctggtacggc ggcgcgatcg gcggcgcgat cggggtgatc ctctgggcct
      721 ggtggcggaa gttcctcaac ctgtggcttc tggacaccgc cgcgccggct ctcgcagcgg
      781 gttatgcggt cggccggatc ggctgccagc tctccggtga cggggattac gggaagccct
      841 gggacggacc gtgggcgatg agctacccgg acggcaccgt cccgaccccc gacggtgtca
      901 ccgtccaccc gaccccgatc tacgagaccc tgaccatggg gctgggcgcg tacatcctct
      961 ggcgcctcag ggaccggttc cggctgggga tacttttcgc gatctacctg atctacgccg
     1021 gaaccgaacg actcctggtc gagttcctgc ggcggaacac cgacgtcttt ctcggactca
     1081 cccaggccca gctcgagagc gtggcgatga tcctcggcgg cattatctgg atcctggtcg
     1141 tcaggcgccg ctacggcagc ctcgcccggc cggccgacca gatgagcgct gccgacgtcc
     1201 ccgcctgacc ggaccgcgaa c
//
LOCUS       DAOQFI010000716         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_89614_length_1220_cov_2.630901, whole genome shotgun sequence.
ACCESSION   DAOQFI010000716 DAOQFI010000000
VERSION     DAOQFI010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89614_length_1220_cov_2.630901"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1220
                     /locus_tag="PLE93_10850"
     CDS             <1..>1220
                     /locus_tag="PLE93_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNH87591.1"
                     /translation="QYGEIKLNIIDTPGHADFGGEVERGLTMVDGVVLLVDASEGPLP
                     QTRFVLGKALEAGLPVILVVNKVDRPDARIGEVVDEVYELFMDLGADGDQIEFPIVYT
                     NGKKGWASLEEGLEGEDLKPLLDLMVEHIPAPEYEEGHPLQAWVTNLDASPYLGRLAL
                     CRVMQGTIKAGSQVAWCRADGTVERAAVSELFVTEALERVSTDQAGPGEIIAVAGIPE
                     VTIGETLSDPEDPRPLPVIKVDEPSLSIVVGINTSPLAGRDGDKVTARQIQNRLDQEL
                     LGNVAIRVLPTDRPDAWEVQGRGELQLVVLLEMMRREGFELTAGQPRVLTRDVDGQMH
                     EPMERLVIDVPEEHVGAVTQLLAARKGRMENMTAQSDSWTRLDYVIPARGLVGFRTEF
                     LTETRGTGILHSIF"
BASE COUNT          210 a          401 c          420 g          189 t
ORIGIN      
        1 ccagtacggc gagatcaagc tgaacatcat cgacaccccc ggccacgccg acttcggcgg
       61 cgaggtcgag cgcggcctga ccatggtcga cggggtggtg cttctggtcg acgcctccga
      121 ggggccgctg ccccagacgc ggttcgtgct cggcaaggcg ctggaggcgg gcctgccggt
      181 gatcctcgtg gtcaacaagg tggaccggcc ggacgcccgc atcggcgagg tcgtggacga
      241 ggtctacgag ctcttcatgg acctcggcgc cgacggggac cagatcgagt tcccgatcgt
      301 ctatacgaac ggcaagaagg gctgggcttc cctcgaggag ggcctcgaag gcgaggacct
      361 caagccgctg ctcgacctga tggttgaaca cattccggcc cccgaatacg aggagggcca
      421 tcctcttcag gcctgggtga ccaacctcga cgcctccccg tacctgggcc ggctcgcact
      481 atgccgggtg atgcagggca cgatcaaggc cggctcgcag gtcgcctggt gccgggccga
      541 cggcacggtc gagcgggccg cggtgtccga gctcttcgtc accgaagcgc ttgagcgggt
      601 atcaacggac caggccgggc cgggcgagat catcgccgtg gccggcattc cggaagtgac
      661 gatcggcgag accctctccg acccggagga tccgcggccg ttgccggtga tcaaggtcga
      721 tgaaccttca ctttcgatcg tggtcggaat caacacttcg ccgctggcgg gccgggacgg
      781 cgacaaggtc accgcccgcc agatccagaa ccggctcgat caggagctgc ttggcaatgt
      841 ggcgatcagg gtgctgccga ccgaccggcc cgatgcctgg gaagtccagg gacggggcga
      901 gctgcagctg gtcgtactgc tcgagatgat gcgacgcgaa ggtttcgagc tcaccgccgg
      961 ccagccgcgg gtgcttaccc gcgatgtcga cggccagatg cacgagccga tggagcgact
     1021 ggtgatcgac gtcccggaag aacacgtcgg cgccgtcacc cagctgctgg ccgcccgcaa
     1081 gggccggatg gagaacatga ctgcccagtc cgacagctgg acccggctcg actatgtgat
     1141 cccggcccgc ggcctggtcg gcttccggac cgagtttctg accgaaaccc gtggcaccgg
     1201 catcctccac agcatctttg
//
LOCUS       DAOQFI010000717         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_89724_length_1219_cov_3.248282, whole genome shotgun sequence.
ACCESSION   DAOQFI010000717 DAOQFI010000000
VERSION     DAOQFI010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89724_length_1219_cov_3.248282"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..>1219
                     /gene="prfA"
                     /locus_tag="PLE93_10855"
     CDS             216..>1219
                     /gene="prfA"
                     /locus_tag="PLE93_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNH87592.1"
                     /translation="MSDPEVIGDRERYAEVGREYRQIQPANALVTEYRTLSDDLEGAR
                     ELLEEGEDEDLEKVVREAPARLEELEEEIRLAMVEPDPNDDKNVIVEIRAGTGGDEAA
                     LFAGDLYKMLTRYAAERGYSTEVLSQSAAEQGGFKDVTFEVRGAGAYSVYKYEGGTHR
                     VQRVPETESQGRIHTSTATVAVLPEAEEVEVDIDQNDLQIDVYRSSGPGGQSVNTTDS
                     AVRITHQPTGIVVSMQDEKSQLQNREKAMRVLRARLYERQLAEQQAAVAAERKAQVGT
                     GERSEKIRTYNFPQGRVTDHRIKLTAHDLDGIMNGNLNQFTDALAADGQVRDMRVMVR
                     L"
BASE COUNT          242 a          374 c          415 g          188 t
ORIGIN      
        1 agcccgaaga gaacgcgtac ctggagccga tcgaaatcgt ggcgtgaccg cagtcgggcc
       61 ggtcttcgcc ggccctccaa attgaacccg ccccttcctc tcacattcac tgacggtagg
      121 aaggggctct gctgtcgttt attcgggaca atggttttgt gattgagaag ttggcggaac
      181 agattgaggc tcgctttgcg gagcttgagc agcagatgag cgaccccgag gtgatcgggg
      241 accgcgagcg gtatgccgag gtcggtcgcg agtaccgcca gattcagccg gccaatgcgc
      301 tggtcaccga gtaccggacc ctgagcgacg atctcgaggg ggcccgggaa ctgctcgagg
      361 agggcgagga cgaggatctc gaaaaggtgg ttcgtgaagc cccggcccgg ctggaagagc
      421 tcgaggagga gatccgcctg gccatggtcg agccggatcc gaacgacgac aagaacgtga
      481 tcgtagaaat ccgggctggc accggtggcg acgaggccgc cctgtttgcc ggtgatctct
      541 acaagatgct cacccgttac gccgccgagc gcggctactc gaccgaggtg ctttcccagt
      601 cggcggccga gcagggtggc ttcaaggacg tgaccttcga ggtccggggc gccggcgcct
      661 actcggttta caagtacgag ggcgggaccc accgggtcca gcgcgtgccg gagaccgaat
      721 cccagggccg gatccacacc tcgaccgcga ccgtggccgt gctgccggag gccgaggagg
      781 tcgaagtgga catcgaccag aacgatcttc agatcgacgt ctaccgctcg tcggggccgg
      841 gcgggcagtc ggtcaacacc accgattcag ccgtccggat cacccatcag ccgaccggca
      901 tcgtcgtgtc gatgcaggac gagaagtccc agcttcaaaa cagggagaag gcgatgcggg
      961 tgttgcgggc ccgtctttac gagcggcaac tggccgaaca gcaggcggcg gtggccgctg
     1021 aacgcaaggc ccaggtcgga accggggagc gctccgagaa gatccgcacc tacaacttcc
     1081 cccagggccg ggtgaccgac caccggatca agctgaccgc ccacgacctt gacggaatca
     1141 tgaacgggaa cctcaaccag ttcaccgacg cgctggccgc cgacggccag gtgcgcgaca
     1201 tgcgcgtgat ggttcgtct
//
LOCUS       DAOQFI010000718         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_90455_length_1214_cov_2.590164, whole genome shotgun sequence.
ACCESSION   DAOQFI010000718 DAOQFI010000000
VERSION     DAOQFI010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90455_length_1214_cov_2.590164"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLE93_10860"
     CDS             <1..374
                     /locus_tag="PLE93_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="BtuF-related (seleno)protein"
                     /protein_id="HNH87593.1"
                     /translation="GHWVPEMIEIAGGRDLVGKAGLKSAEHGWDELANLDPDLTVVMP
                     CGYYVDEAATQADDYMDRIRSTGAERTVAVDAAASFSRPGPRLIDGIELLAGLLHPEV
                     FPLEEGQIQYREMQTGDRFGG"
     gene            complement(315..1184)
                     /locus_tag="PLE93_10865"
     CDS             complement(315..1184)
                     /locus_tag="PLE93_10865"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNH87594.1"
                     /translation="MATCYRHPNVETGVSCSSCGRPICPDCMTTTPVGMRCPECATEK
                     TRVRSGPAATSQVGGYPATIALIAINVVVFLAEMATGSGGLSSTSSDLISDFGLRGAQ
                     VADGETYRLVTSGFLHAGFMHIAFNMIALYFLGRVLEPSIGTVRFLMVYFASLLAGSF
                     GAVLLSDNLALTVGASGAVFGLFAATFIIAYGRGLGHVANEIGLIIGINLLLTFTISG
                     ISIGGHLGGLIGGGLCGILVIAGERGYLGSNARLLEYGGMIAIAAVSVFAAISAAEPV
                     PGLHFAVLNLPFL"
BASE COUNT          254 a          371 c          402 g          187 t
ORIGIN      
        1 gcggccactg ggtgccggaa atgatcgaga tcgccggcgg ccgcgacctg gtcggcaaag
       61 caggcctgaa gtcagctgaa catggatggg acgaactggc caacctcgat ccggacctga
      121 ccgtggtcat gccctgcggc tactacgtcg atgaggccgc cacccaggcc gacgactaca
      181 tggaccggat caggtcaacc ggagcagagc ggaccgtcgc ggttgacgcc gccgcctcgt
      241 tctcaaggcc aggccctcgc ctcatcgacg gaattgaatt gctggcgggt ctgttgcatc
      301 ccgaggtttt tcctctagag gaagggcaga ttcagtaccg cgaaatgcag accggggacc
      361 ggttcggcgg ctgagatcgc ggcgaatacc gaaactgcgg cgatcgcgat catccctccg
      421 tactcgagca ggcgggcatt cgaacccagg taaccacgct cgccggcgat gaccagaatt
      481 ccgcacaacc cgccgccgat cagaccaccg agatggccgc cgatgctgat gccgctgatc
      541 gtgaaggtga ggagcaggtt gataccgatg atcaatccga tctcgttcgc cacatggccg
      601 agcccacggc cgtaggcgat gatgaaagtc gccgcgaaga gcccgaagac ggcgccggag
      661 gcaccgacgg tcagggcgag gttgtcgctg agcaggacgg ccccgaaaga accggccaga
      721 agcgacgcga agtagaccat caggaagcgc accgtgccga tcgaaggctc gagtacccgt
      781 cccaggaagt aaagggcgat catgttgaag gcgatgtgca tgaagccggc gtggagaaaa
      841 ccgctggtga ccagccggta agtctcgccg tcggccacct gtgcgccgcg caggccgaaa
      901 tcgctgatca gatctgaact ggtcgagctg agaccaccgg atccggtcgc catttccgcc
      961 aggaatacga ccacgttgat cgcgatcagg gcgatcgtgg ccgggtagcc gccgacctgg
     1021 gaggttgccg caggaccgct gcggacccgg gtcttttcgg tcgcacattc cgggcagcgc
     1081 atgccgaccg gggtcgtcgt catgcagtcg gggcagatcg gacgaccgca gctcgaacat
     1141 gaaacgcctg tctcgacgtt cgggtggcgg taacaggtag ccaaggccgt caaaccctat
     1201 cggtgtgccc gggt
//
LOCUS       DAOQFI010000719         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_91539_length_1207_cov_2.394097, whole genome shotgun sequence.
ACCESSION   DAOQFI010000719 DAOQFI010000000
VERSION     DAOQFI010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91539_length_1207_cov_2.394097"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..598
                     /locus_tag="PLE93_10870"
     CDS             206..598
                     /locus_tag="PLE93_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87595.1"
                     /translation="MNPAYRHLEEPVKIGDFTLGQIAGFLVAAMLAMLWGFVISPLPP
                     IWSLFTAIYIGGLPAMAVLFASTTDFDAFRMVKAYVLFQRRPGRFVPGAGRLEDRGYV
                     VKKPPVEKHRPVNTKTDEILIGEALWGD"
     gene            631..>1207
                     /locus_tag="PLE93_10875"
     CDS             631..>1207
                     /locus_tag="PLE93_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87596.1"
                     /translation="MPEAGEIFEIDALDRDGLMIRSDGTFIRMLEVTPRNPELLSIEE
                     AQAMSATYAQMLARLRPKQSIQFIIDSRPVHVDRVLEASRKEVERVAGPAPTTPELRR
                     SKTKLERNRWQMFGAMSQSIHQRSDSEGAVEMRAYLVLPYTPLSESDNFGVRDMIPGL
                     KGKTEEFSERERAAHERGVNESRNHAETIRAD"
BASE COUNT          284 a          363 c          373 g          187 t
ORIGIN      
        1 acaggtccgt gaatcagtga tcaccggcgg gcaaggctac gggggaggcg gcaacaccca
       61 aacgccgagg ccggcacccg cacaagcgaa cggcaacggc aacggcaacg gcggcggaac
      121 cccgcggccg ggatccggaa ccaaagccgt accggacccg accgaaggac ataaacgacc
      181 tgcctccaag aggggagaaa gctaaatgaa cccggcctac aggcaccttg aggaaccggt
      241 caagatcggt gacttcacac tcggccagat cgcaggtttc ctggttgcgg cgatgctcgc
      301 aatgttgtgg ggattcgtga tcagtccgct cccgccgatc tggtcgctgt tcaccgccat
      361 ttacatcggc gggcttccgg cgatggcggt cctgttcgcc tcaaccaccg actttgacgc
      421 gttccggatg gtgaaggcct atgtgctctt tcaacgacgt ccgggccggt tcgtgccagg
      481 tgccggccgt ctcgaggacc gcggctacgt ggtcaagaag ccgccggtcg aaaagcatcg
      541 tccggtcaac acgaaaaccg acgaaatcct gattggagaa gcactgtggg gggactagcc
      601 gaaaagatcc ggccaaccga agttgaggac atgcccgaag cgggcgagat cttcgagatt
      661 gacgcgctcg accgggacgg actgatgatc cgctccgacg gcacgttcat ccggatgctc
      721 gaggtgaccc ctcgaaaccc ggagctcttg tcgatcgagg aagcgcaggc aatgtcggcc
      781 acctacgcgc aaatgctggc gaggctccgg ccgaagcaaa gcatccagtt catcatcgac
      841 tcgcggccag tccatgtcga ccgggtactt gaagcgtccc gaaaggaagt cgaaagggtt
      901 gccggtccgg ccccgacaac accggagctt cggcgctcga aaacgaaact ggaacggaac
      961 cgctggcaga tgttcggggc aatgagccag tcaattcacc aacgctccga cagcgaaggt
     1021 gccgttgaga tgcgcgcata cctggttctg ccttacacgc cactctcgga atccgacaat
     1081 ttcggggtca gggacatgat tcccggactc aaaggcaaga cggaagaatt ctcagaaagg
     1141 gaacgggccg cccatgagag aggtgtcaac gaatcccgaa accatgcgga aacgatccgg
     1201 gccgaca
//
LOCUS       DAOQFI010000720         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_91765_length_1205_cov_3.821739, whole genome shotgun sequence.
ACCESSION   DAOQFI010000720 DAOQFI010000000
VERSION     DAOQFI010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91765_length_1205_cov_3.821739"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1205)
                     /locus_tag="PLE93_10880"
     CDS             complement(<1..>1205)
                     /locus_tag="PLE93_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNH87597.1"
                     /translation="GFDPEKIWITVFGGDDELGLGPDEEAIGIWKGLGVPDERIVKLP
                     RSENFWQGGPTGPCGPCSEMYLDRGLDFGSVDDRPGDDSDRFLEYWNHVFMSYDQSED
                     GKLTPLPKKNIDTGMGLERMASILQGVESVYDTDLFRPLIELAEEKSGLKYGEDEAVT
                     RAMRVISDHSRGMTFLLAAGVVPSNEDRGYVLRRVMRRAIQRGRALGLEGSWLGDFTD
                     RTIEMMGDAHPQVVAERERIDRWVKAEEESFGRTLDRGTALLEDLVDRALTEKNSWIS
                     ADDAFKLHDTFGFPYDLTREMVEERGLSVDDQGFEELMEQQRNQSRSNASGGSEGADR
                     HGRILDFAGQGAESEFVGYEYMRTETGIGSLQAVNGSALAKLEQSPFYAEGGGQVAVD
                     VGGGAAPIA"
BASE COUNT          194 a          419 c          377 g          215 t
ORIGIN      
        1 cgcaattgga gcggcgcctc caccaacatc aacggcgacc tggccgccac cctcggcgta
       61 gaagggactc tgttcgagct tggcgagagc cgagccgttg accgcctgaa gcgacccgat
      121 tccggtttct gtccgcatgt actcgtagcc gacgaactcc gattcggcgc cctggccggc
      181 gaagtcgagg atccgcccgt gacgatccgc cccctcggag ccgccggagg cgttgctgcg
      241 ggactggttg cgctgctgtt ccatcaactc ttcgaagccc tggtcatcaa ccgaaaggcc
      301 ccgctcctcg accatctcgc gggtcaggtc gtaggggaag ccgaaagtgt catggagctt
      361 gaaggcgtcg tcggcactga tccacgagtt cttctccgtg agggcccgat cgaccagatc
      421 ctcgagcagg gcggtgccgc ggtcgagggt gcgaccaaag ctctcctcct cggccttgac
      481 ccagcgatcg atccgctccc gctccgccac cacctgcggg tgggcgtcac ccatcatctc
      541 gatcgtgcgg tcggtgaagt cgcccagcca cgagccttcg agaccgagcg cccggccacg
      601 ctggatcgcc cgccgcatca cccgccgaag cacgtacccg cggtcttcgt tcgagggcac
      661 caccccggcg gcgaggagga aggtcatgcc gcggctgtgg tcgctgatca cccgcatcgc
      721 ccttgtaacg gcctcgtctt cgccgtactt caaaccggac ttctcctcgg ccagttcgat
      781 caggggccgg aagaggtcgg tgtcatagac cgactccaca ccctgcagga tcgaggccat
      841 gcgctcgagg cccatgccgg tgtcgatgtt cttcttcggc agcggggtca gtttgccgtc
      901 ctcggactgg tcgtagctca tgaagacgtg gttccagtac tcgaggaagc ggtcgctgtc
      961 atcgccggga cggtcgtcca cggaaccgaa gtcgaggccg cggtccagat acatctcgga
     1021 gcaggggccg caggggccgg tcggcccgcc ctgccagaag ttctcggagc ggggcagctt
     1081 cacgatccgt tcgtccggga ccccgaggcc cttccagatc ccgatcgcct cctcgtcggg
     1141 tccgagcccg agctcgtcgt ccccgccgaa gacggtgatc cagatctttt ccgggtcgaa
     1201 tccga
//
LOCUS       DAOQFI010000721         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_91957_length_1204_cov_2.714534, whole genome shotgun sequence.
ACCESSION   DAOQFI010000721 DAOQFI010000000
VERSION     DAOQFI010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91957_length_1204_cov_2.714534"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLE93_10885"
     CDS             <1..260
                     /locus_tag="PLE93_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87598.1"
                     /translation="APGPSDWKPGMRSRGNLLLIDGDFKLGAFEVVDATQWRRDGRGG
                     SFEDNDLDPDARASTAARARATAACAPRPTRLEKLKATASS"
     gene            284..>1204
                     /locus_tag="PLE93_10890"
     CDS             284..>1204
                     /locus_tag="PLE93_10890"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNH87599.1"
                     /translation="MRDRQGGMLAGVCSGIALRLKVDPTLVRVVAVVLTVITGGVAAI
                     GYGLLWVALPVAGEEAIVRRFKPRQGSREKVVAKPAPARPFKGSWAVAAGAGFVTLGL
                     LFVFRETGVWWSDSLVWPLVIAAAGAALIWRQFSQSSRTILGPFRGGVGIALLLGGAV
                     LFLYINGALSAGGDVALAGLVAVIVIGLLAAPIWARSARQLSTERAERIRSEERAEVA
                     AHLHDSVLQTLALIQKNADDSRQVSTLARSQERELRDWLSGKTDRDRADSLAESLKAV
                     AAEVEERHQVPIDAVTVGDRPLDENSIALVA"
BASE COUNT          173 a          432 c          414 g          185 t
ORIGIN      
        1 gcgctcccgg cccgtccgac tggaagcccg gcatgcgaag cagaggcaac ctgctgctga
       61 tcgacggcga tttcaaactc ggagccttcg aggtcgtgga cgcgacccag tggcgtcggg
      121 atggccgcgg cggcagcttc gaagacaacg atctcgatcc cgatgcgcgg gcatcgaccg
      181 ccgcacgcgc acgggcgacc gcagcttgcg cacctcgccc gacgcgcctc gagaagctga
      241 aggcgacggc cagctcctga acgaaccacc cgacaagcgg ctcatgcgcg accgccaggg
      301 cgggatgctg gccggggtct gctccggcat cgcgctgcgg ctcaaggtcg acccgaccct
      361 ggtccgggtt gtcgcggtgg tgttgaccgt gatcaccggc ggggtcgcgg cgatcggcta
      421 cggcctgctc tgggttgccc ttccggtggc cggcgaagag gcgatcgtcc gccgcttcaa
      481 accaaggcag ggcagccggg agaaggtcgt cgcaaaaccg gctccggcgc gacccttcaa
      541 aggctcctgg gcggttgccg ccggggccgg tttcgtcacc ctcgggctgc tcttcgtctt
      601 ccgtgagacc ggggtctggt ggtcggactc cctggtctgg ccgctggtca tcgccgccgc
      661 cggggcggcg ctcatctggc gccagttcag ccagtcatcc cgcaccatcc tcggaccctt
      721 ccggggcggg gtcggcatcg ccctgctgct gggcggggcc gtcctcttcc tctacataaa
      781 cggggccctc agcgccggtg gcgacgtcgc cctggctggg ctggtcgcgg tgatcgtgat
      841 cggtctcctc gccgccccga tctgggcccg ctcggcccgc cagctcagca ccgagagagc
      901 cgagcggatc cgctccgagg aaagggccga agtcgccgcc cacctgcatg attcggtcct
      961 ccagaccctt gccctgatcc agaagaacgc cgacgactcc cgtcaggtct cgaccctcgc
     1021 ccgctcgcag gagcgggaac tgcgcgactg gctctcgggc aagaccgacc gcgaccgggc
     1081 cgactcgctg gccgagtcgc tgaaggcggt cgccgccgaa gtcgaggaga ggcatcaggt
     1141 gccgatcgat gcggtcaccg tcggtgaccg gcccctcgac gagaactcga tcgccctggt
     1201 cgcc
//
LOCUS       DAOQFI010000722         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_92547_length_1200_cov_2.571179, whole genome shotgun sequence.
ACCESSION   DAOQFI010000722 DAOQFI010000000
VERSION     DAOQFI010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92547_length_1200_cov_2.571179"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLE93_10895"
     CDS             <1..829
                     /locus_tag="PLE93_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87600.1"
                     /translation="IATIQGSLPTVSITGPGDGLTFDFNSAHFLGLTCGTGAGPDLES
                     CLARDQGPDGETNLVTGDRLPTDQPGTHTLTATATNELGLTATDTVTYEVGPGCFADG
                     VRLASVQPRGAKVKLLGAADPSSAGRQARILRAGKRAGTATVTGDGRIEATVSAPKSK
                     RLRPGTRYRLVVGGSRSNPMKPSGAIRVLSRKPLPDADLIKGKLAGVRKQGRFTLRAR
                     PLCGGPATSRQVSFNRSGVFSVRIGFTSATRTFSIRRSGGEVPLPVVMPAARFITGN"
     gene            complement(837..>1200)
                     /locus_tag="PLE93_10900"
     CDS             complement(837..>1200)
                     /locus_tag="PLE93_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNH87601.1"
                     /translation="EDARKAVDAGVDGVAVSNHGGRQLDAVPSTAAALPGVAEAVGDE
                     TTVLADGGIRNGLDMVKMLALGAEAVIIGRAWAWAVAAEGQAGVEKMLRVLKSDADVA
                     LALTGQNSVAGLGPDSLV"
BASE COUNT          186 a          443 c          400 g          171 t
ORIGIN      
        1 gatcgccacg attcagggca gccttccgac cgtgtcgatc acgggccccg gggacgggct
       61 cacgttcgac ttcaactcgg ctcatttcct tgggcttacc tgcggtaccg gggccgggcc
      121 ggatctcgag tcctgcctcg ccagggacca gggcccggac ggggagacga atctggtcac
      181 cggcgaccgg ctgccgaccg atcagccggg cacccacacc ctgaccgcga ccgcgaccaa
      241 cgagctgggg ctgaccgcga ctgacacggt cacctacgag gtcgggccgg gctgcttcgc
      301 tgacggggtc aggctggcct cggtccagcc ccggggagca aaggtgaagc tgctcggtgc
      361 cgccgacccc tccagcgccg gccgccaggc caggatcctg cgggccggca agcgggccgg
      421 caccgcgacc gtcaccggag acggcaggat cgaagcgacg gtcagcgccc cgaaatcgaa
      481 gcggcttcgc cccgggacgc gctaccggct ggtcgtcggt ggaagcaggt cgaatccgat
      541 gaagccaagc ggagcgatca gggtcctttc ccgcaagccg cttccggatg ccgacctgat
      601 caagggaaaa ctggccgggg tcaggaagca gggccggttt acactgcggg cccgcccgct
      661 ctgcggcggt ccggccactt cgcgccaggt cagcttcaac cgcagcggcg tcttttcggt
      721 ccggatcggt ttcacgagcg ccacccggac cttctcgatc aggcggtccg gcggggaagt
      781 ccccctcccg gtggtcatgc cggccgcccg gttcatcacc gggaactgaa ccccgctcag
      841 accagcgagt ccggacccag gccggcgacc gagttctgac cggtcagcgc gagggcgacg
      901 tcggcgtcgg acttcagcac ccggagcatc ttctccaccc cggcctggcc ttccgcggca
      961 accgcccagg cccaggcccg gccaatgatc acggcttcgg ccccgagggc gagcatcttg
     1021 accatgtcga ggccgtttct tatgccgccg tcggcaagca ccgtggtctc gtccccgacc
     1081 gcttcggcaa ccccgggcag ggcggctgcg gtcgatggca ccgcgtcaag ctgccgccca
     1141 ccgtgattgg agacggccac cccgtcaacc ccggcgtcga ccgccttgcg ggcatcctcc
//
LOCUS       DAOQFI010000723         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_92740_length_1199_cov_2.164336, whole genome shotgun sequence.
ACCESSION   DAOQFI010000723 DAOQFI010000000
VERSION     DAOQFI010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92740_length_1199_cov_2.164336"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLE93_10905"
     CDS             complement(<1..792)
                     /locus_tag="PLE93_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNH87602.1"
                     /translation="MESLLAEYVADRSHESEPAPGSFTGAAGGAACGDLARISLVVEN
                     GVVSRTCFGTEGCAATRAACACVCELIEGESVLEAARIGAARIEEELGGLSPARRHAA
                     MLAGDALHRALSAVASSGELISPPVEGRVLVAVSGGVDSAVAALGEKEAGAEVVAVTV
                     KLWADPDTDGTKACCSPEAVLGARSLTHDLGIPHFTLDLEGPFRERVVGEFLRGYGEG
                     RTPNPCVLCNGEVRIAAMVDLADRLGAESLVTGHYARVVEDEGGAL"
     gene            841..>1199
                     /locus_tag="PLE93_10910"
     CDS             841..>1199
                     /locus_tag="PLE93_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH87603.1"
                     /translation="MSKGWNADDITDQTGRTAVVTGGNSGIGLITARELLRHGARVII
                     GCRNEDKGKAAIEQIASDLGTPGIATGIEVRRLDLASLDSVREFAESVDADLPDGLDL
                     LINNAGVMAPPRLLTED"
BASE COUNT          209 a          433 c          390 g          167 t
ORIGIN      
        1 cagggcgccg ccttcgtcct cgacgacccg ggcgtagtga ccggtaacaa ggctttcggc
       61 gccgagccgg tcggccaggt cgaccatggc cgcgatcctc acctcgccgt tgcagagcac
      121 gcaggggttc ggggttctcc cctcgccgta gccgcgcagg aattcgccga ccacccgctc
      181 gcggaacggg ccttcgaggt cgagagtgaa gtgcgggatg ccgaggtcgt gagtcagaga
      241 gcgggcgccg agcactgctt cgggcgagca gcaggccttg gtgccgtccg tgtccgggtc
      301 agcccagagc ttcacggtca ccgcgacgac ttcggccccg gcctccttct cgcccagtgc
      361 ggccacggcg ctgtcaacgc cacccgagac cgcgaccagc accctgccct cgaccggtgg
      421 tgaaatcagc tcgcccgagg aagcgacggc cgaaagcgcc cggtgaagcg cgtcgccggc
      481 cagcatcgcc gcgtgacgcc gggccgggct gagcccgccg agctcctcct cgatccgggc
      541 cgccccgatc cgggccgcct ccagcacgct ttcaccctcg atcagctcac aaacgcaggc
      601 gcaggcggcc ctggttgcag cgcagccctc ggttccgaaa caggtgcgcg aaaccacgcc
      661 attttcgacc accagggaaa tccgggcgag atcaccgcag gcggcgccgc cggcggcccc
      721 ggtgaacgat ccgggggccg gttccgactc atgcgagcgg tcggcgacat attcggcgag
      781 cagcgactcc atcccctaga ggttaaatgg tcgattcccc cagcggatac gctgccccgc
      841 atgagcaaag gctggaacgc agatgacatc accgatcaga cgggtcgcac cgcggtcgtc
      901 accgggggca acagtggcat cgggctgatc accgcccgtg aactgctgcg gcacggagcg
      961 cgggtgatca tcggctgccg aaatgaagac aagggcaagg ccgcgatcga gcagatcgcc
     1021 tccgacctcg gcacgccagg tatcgccacc gggatcgaag tgcgacggct cgacctcgcc
     1081 tccctggatt cggtccgcga attcgccgag tctgtcgacg ccgacctgcc cgacggactt
     1141 gacctcctga tcaacaacgc cggcgtcatg gctccgcccc ggctgctcac ggaagacaa
//
LOCUS       DAOQFI010000724         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_93013_length_1197_cov_3.146235, whole genome shotgun sequence.
ACCESSION   DAOQFI010000724 DAOQFI010000000
VERSION     DAOQFI010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93013_length_1197_cov_3.146235"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1197
                     /locus_tag="PLE93_10915"
     CDS             <1..>1197
                     /locus_tag="PLE93_10915"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87604.1"
                     /translation="VLPVGGIKEKILAAHRAGIRTIILPSKNERDIEEVPGHVKRKLA
                     FIYVEHMDQVIASALREVAPLNDGEAKRNGKRKKTAKSSRKLAPQKSLCDVPAGVDLA
                     SSDKCDFIAGPGNACLAPFPNDFYTREDEGSTTGKRLDLDPDSTPANSSEGKHIAVNV
                     LNQSDGFSPGPLLSVRIPGMDNQAAFDQSGIVSIKEMSAAYAPAQPVVLIDAATGERQ
                     LIWAELDSQATTDADRNLIIRPAKNLTNGHRYIVAIRNMKDSSGDPIQAPAAFRLYRD
                     TDRTGDNRIESRRAHFESIFKTLGDAGIQRKSLYLAWDFTVASTENITGRMLSIRDRA
                     FAGLGDTNLTDGVVQGDAPAYHIDSVTDYTGNHTGAGTEDVRVVKGTVTVPCFLNQAG
                     CPSGST"
BASE COUNT          232 a          424 c          365 g          176 t
ORIGIN      
        1 cgtgctgccg gtcggcggca tcaaggagaa gatcctcgcc gcccaccgcg ccggcatccg
       61 cacgatcatc ctgccgtcca agaacgagcg cgatatcgaa gaggtgccgg gccacgtcaa
      121 gcgcaagctg gcgttcatct acgtcgagca catggaccag gtgatcgcca gcgcactgcg
      181 cgaggtcgct ccactcaacg atggagaggc gaagcgcaac ggcaagcgca agaagaccgc
      241 aaagtccagc cgcaagctgg ccccccagaa gtcgctctgc gacgtgccgg ctggcgttga
      301 cctcgccagt tccgacaagt gtgacttcat cgccggaccg ggcaatgcct gtctggcccc
      361 cttcccgaac gacttctaca ccagggagga cgagggctcg accaccggaa agcggctcga
      421 cctcgacccc gattccacgc cggcgaactc ctccgagggc aagcacatcg ccgtgaacgt
      481 gttgaatcag tccgacgggt tcagccccgg tccgctgctt tcggtgcgga tccccggcat
      541 ggacaaccag gccgccttcg accagtccgg gatcgtttcg atcaaggaga tgagcgccgc
      601 ctacgccccc gcccagccgg ttgtgctgat cgacgcggcg accggcgagc gccagctgat
      661 ctgggccgag ctcgacagtc aggcgaccac ggatgcggat cgcaatctga tcatccggcc
      721 cgcgaagaac ctgacgaacg gccaccggta catcgtcgcg atccgcaaca tgaaggattc
      781 ctccggtgac ccgatccagg ctccggccgc gttccggctg taccgcgaca ccgaccgcac
      841 cggcgacaac aggatcgaga gccgccgggc ccacttcgag tcgatcttca agaccctcgg
      901 cgatgccgga atccagcgga agtcgctcta cctggcctgg gacttcacgg tggcgagcac
      961 cgagaacatc accggccgca tgctttcaat ccgggaccgg gccttcgccg ggctcgggga
     1021 caccaacctg accgacggcg tggtccaggg cgatgccccg gcctaccaca tcgattcggt
     1081 aaccgactac acgggcaatc acactggcgc cggcaccgag gacgtccggg tggtcaaggg
     1141 caccgtcacg gtgccctgct tcctgaacca ggccggttgc ccgtccggtt ccacgtt
//
LOCUS       DAOQFI010000725         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_93296_length_1195_cov_3.315789, whole genome shotgun sequence.
ACCESSION   DAOQFI010000725 DAOQFI010000000
VERSION     DAOQFI010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93296_length_1195_cov_3.315789"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1195)
                     /locus_tag="PLE93_10920"
     CDS             complement(<1..>1195)
                     /locus_tag="PLE93_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87605.1"
                     /translation="VTDVMRNIMEELREVGIPDRNTDLADYLEKAPTVVSWGTRAVTE
                     LFDEHYLSERSRAVILGLAGDYATPPSRAPVTIHAGVLDHYLQEGAFYVRGGGQELAG
                     HLVDLIHSYGGRVRTHAEVEHIATASTEDGGHRVTGVELKGGEVIDTGNVIATGDIKH
                     LFLDLLDSEAVPEGIREQVKAYRMATPLFTVYLGLDFDITERMPATNYWLHSRYDQEA
                     YYDPIAAGEFDTDPPVYISSASAKDPGHEGHAPEGCSTVELMTWATPDPEAWGVDESE
                     DAPKYSRQAAYREAKDRLTEQLIDRAEDQLGEIRSHILWKEAATPMTQTRYTLSTGGS
                     SYGIELATDQFGPLRLEYRTPIEGLVLGGVSSRRGHGIVGAMSGGVESASAVLGRNLR
                     AEVAGG"
BASE COUNT          182 a          387 c          398 g          228 t
ORIGIN      
        1 gccgccggcg acctcggccc ggaggttccg gccgaggacg gccgaggcgg attccacccc
       61 gccgctcatc gccccgacga tgccgtgacc gcggcggctc gagactccgc caaggaccag
      121 gccctcgatc ggtgttctgt actcgaggcg gagcggaccg aactggtcgg tggccagttc
      181 gatcccgtag gaggaaccac cggtggaaag cgtgtacctg gtctgggtca tcggcgtcgc
      241 cgcttccttc cagaggatgt gcgaacggat ctcgccgagc tgatcctcgg cccggtcgat
      301 cagctgctcg gtcaggcggt ctttcgcctc gcggtaggcg gcttgccggc tgtacttcgg
      361 ggcatcttcg ctctcgtcga ctccccaggc ttcggggtcg ggggtggccc aggtcatcag
      421 ctcgacggtt gaacatcctt cgggcgcatg accttcatga cccggatcct tggcgctggc
      481 cgaggagatg taaaccgggg ggtcggtgtc aaactcgccg gccgcgatcg ggtcgtagta
      541 agcctcctgg tcatagcggc tgtgcagcca gtaattggtg gctggcatcc tctccgtgat
      601 gtcgaagtcg agccccagat agaccgtgaa gaggggggtc gccatgcgat aggccttgac
      661 ctgttcgcgg atcccttccg gcacggcctc ggagtcgagc aggtcgagga agaggtgctt
      721 gatgtctccg gtcgcgatca cgttcccggt atcgatcacc tcgccgcctt tcagctcgac
      781 cccggtcacg cggtggccgc cgtcttcggt cgaagcggtg gcgatgtgct cgacttcggc
      841 gtgagtgcga acccttcccc cgtagctgtg aatgagatcg acgaggtgac cggccagctc
      901 ctgcccgccg ccccggacgt agaaggcacc ctcctgcagg tagtggtcga gaaccccggc
      961 gtgaatggtc accggagccc tcgacggagg cgtggcgtag tcgcctgcga ggcccaggat
     1021 cactgcccgc gagcgctcac tcagatagtg ctcgtcgaaa agttcggtca ccgctctcgt
     1081 accccagctg accaccgtcg gcgccttctc gaggtagtcg gcgaggtccg tgttccgatc
     1141 ggggataccg acctcgcgaa gctcctccat gatgttgcgc atcacgtcgg tgact
//
LOCUS       DAOQFI010000726         1194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_93436_length_1194_cov_4.172081, whole genome shotgun sequence.
ACCESSION   DAOQFI010000726 DAOQFI010000000
VERSION     DAOQFI010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93436_length_1194_cov_4.172081"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1091
                     /gene="meaB"
                     /locus_tag="PLE93_10925"
     CDS             90..1091
                     /gene="meaB"
                     /locus_tag="PLE93_10925"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNH87606.1"
                     /translation="MGRTSPSVEELKEGIARGDRGALSRAITLAESRRPDDQEHAQEL
                     IADLLPETGEAIRVGITGVPGAGKSTAIEALGLELIRHGHKVAVLTIDPTSARTGGSI
                     LGDKTRMHKLSVEEDVFIRPSPSGGVLGGVARKTREAMLLCEAAGFDVIIVESVGVGQ
                     SEAELAEMVDCLMLLLVPGAGDELQGIKRGIMELADLIVVNKADGDRVALAKRARGDY
                     RHALRMLPPSTPGWETPVQIASAAEGTGLKEIWESVEAHRALLEESGLLHERRRQQTE
                     RWLDSMIEEAVLAAVHSRQGVDATIKQARIDVDAEKITVPQATRKVIEAAGLAGEQK"
BASE COUNT          205 a          396 c          422 g          171 t
ORIGIN      
        1 ggcaccgtga tcggcgaagc agcgaccgag ctgctgctgc tgctcggcga gaggctcgac
       61 ctggacatcc agaccggctc cccgtcgtaa tgggcagaac cagcccttcg gtcgaggagc
      121 tgaaggaggg catcgcccgc ggtgaccgcg gggcgctctc aagggcgatc accctggctg
      181 aaagccgccg gcccgatgac caggagcacg cccaggagct gatcgccgac ctgctgccgg
      241 aaaccggtga ggcgatccgg gtcgggatca cgggcgtgcc cggggccggc aagtcgaccg
      301 cgatcgaggc gctcggtctt gagctgattc gccacggtca caaggtcgcg gtgctcacga
      361 tcgatccgac ctcggcccgc accggcggtt cgatcctcgg tgacaagacc cgcatgcaca
      421 agctgagcgt cgaggaggat gtcttcatcc gtccttctcc ctcgggtggg gtgctcggcg
      481 gggtggcccg caagacccgt gaggcgatgc tgctctgcga ggcggccggt ttcgacgtga
      541 tcatcgtcga gtcggtcggg gtcggccagt ccgaggcgga gctggcggag atggtcgact
      601 gcctgatgct gctactggtt cccggcgccg gcgacgagct tcaggggatc aagcggggca
      661 tcatggaact ggccgacctg atcgtggtca acaaggccga cggtgaccgg gtggcgctgg
      721 ccaagcgggc ccggggtgac taccgccacg cgctccgcat gctgccgccc tcgactcccg
      781 gctgggagac cccggtccag atcgcctcgg ccgccgaggg caccggactc aaggagatct
      841 gggaatcggt cgaggcccac cgggcactgc tcgaggaaag cggcctgctc cacgaacgcc
      901 gccgccagca gaccgagcgc tggctcgatt cgatgatcga ggaagccgtc ctcgccgccg
      961 tccattcccg gcagggggtg gacgcgacga tcaaacaggc ccgcattgac gtggacgccg
     1021 agaagatcac cgtcccccag gccacccgca aggtgatcga agccgccggt ctcgcagggg
     1081 agcagaaatg atcggaccga ccgatgtcga gatccgcgtg ctcggctgcc tgatcgagaa
     1141 gcagcggacg accccggacc agtacccgct gaccctgaac tcgctgcggc tggc
//
LOCUS       DAOQFI010000727         1194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_93456_length_1194_cov_3.129939, whole genome shotgun sequence.
ACCESSION   DAOQFI010000727 DAOQFI010000000
VERSION     DAOQFI010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93456_length_1194_cov_3.129939"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>1194
                     /locus_tag="PLE93_10930"
     CDS             129..>1194
                     /locus_tag="PLE93_10930"
                     /inference="COORDINATES: protein motif:HMM:NF017073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HNH87607.1"
                     /translation="MGRPPSRQERQKRLKTRALPLVGVALISFIVGMVKGCPGSPNRD
                     AAEQYVEYWAEGNYVAMYDMLSTKSREGITQERFIARYGEASAEATIESIEQSDAEGD
                     ETNAEIPMEVDTLAFGTIDQSLKVTFGSDGIAWQNSLLFPGLELNEELTRVTKLPGRA
                     KILAEDGQVMAEGPVDAREHPLGDSMIDVTGVTGPPDQEEGPDLTSLALGYEPGEDTG
                     ISGLELAYNARLTGTPGGTLFAKPIEGGTDGQGRELGSGTPAPAKPLKTTIDPDLQES
                     AVANLGGVSGGVTVLDAKKGFIKALAGSAYSVLQPPGSTMKMVTATAALETGAATMDS
                     SYDYVTEAPADGRMIQNAHGE"
BASE COUNT          248 a          391 c          388 g          167 t
ORIGIN      
        1 gcaagcagga aggtgaaggc ggcgatgccc cagtccagcg ctgtcacccg gccaatttag
       61 cccccgggcg gcaccgatgg tcagccgcaa catctaacca ccggcgaccc cggtattttt
      121 cagcccagat ggggcgtcct ccttcacgcc aggaacggca aaaacgactc aagacgaggg
      181 ctttgcctct ggtcggagtc gcgctgatct ctttcatcgt cggaatggtc aagggatgcc
      241 ccggcagccc caaccgtgat gccgccgagc agtacgtcga gtactgggcc gaaggcaatt
      301 acgtcgcgat gtacgacatg ctcagcacca agtcccggga agggatcacc caggagcgat
      361 tcatcgccag gtacggggaa gcttcggccg aggcgacgat cgagagcatc gaacaaagcg
      421 atgccgaagg cgatgaaacc aacgccgaaa tcccgatgga agtggacact ctcgctttcg
      481 ggacaatcga ccagtcgctc aaggtcacct tcggttcgga cggaatcgcc tggcagaaca
      541 gcctgctctt ccccgggctc gaactgaacg aagagctgac cagggtcacc aagctgcccg
      601 gccgggcgaa gatcctcgcc gaagacgggc aggtgatggc cgagggtccg gtcgacgccc
      661 gcgagcatcc gctcggagac tcgatgatcg acgtgaccgg ggtcaccgga ccgcccgacc
      721 aggaagaggg cccggatttg accagcctcg ccctcggtta cgaacccggc gaggacaccg
      781 gcatcagcgg gcttgaactc gcttacaacg cgcggctcac cggcaccccc gggggcaccc
      841 tgttcgccaa gccgatcgag ggtggaaccg atggccaggg gcgcgagctc ggcagcggca
      901 ccccggcccc ggccaaaccg ctgaagacca cgatcgaccc ggatctgcag gagtcggcgg
      961 tggcaaacct cggcggggtc tccggcggcg tgacggtgct cgacgcgaag aagggcttca
     1021 tcaaggctct ggccggttcc gcctattcgg tgctgcagcc gcccggatcg acgatgaaga
     1081 tggtcaccgc caccgcggcc ctggaaacgg gggccgcgac catggattcc tcatacgact
     1141 acgtgactga ggccccggcc gacgggcgca tgatccagaa cgcccacggg gagg
//
LOCUS       DAOQFI010000728         1194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_93484_length_1194_cov_2.648815, whole genome shotgun sequence.
ACCESSION   DAOQFI010000728 DAOQFI010000000
VERSION     DAOQFI010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93484_length_1194_cov_2.648815"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLE93_10935"
     CDS             <1..507
                     /locus_tag="PLE93_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNH87608.1"
                     /translation="GQAGQFEEPGIITVEGGETRSHSVANGLAAITADGDPGMVVVHD
                     AARPLVSPALIDAAVIGLDSDPELHALIAAAPMTDTVKRLGEDGIVVETLDRSELVSV
                     QTPQVFRIPNLQAAIAAGDLSAATDDASLVEAVGGSVGVIESPSGNIKVTVPADLELA
                     ALLLERSR"
     gene            504..>1194
                     /locus_tag="PLE93_10940"
     CDS             504..>1194
                     /locus_tag="PLE93_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNH87609.1"
                     /translation="MTDPVITDYHLHLRGDELSARAEDHFTTENIARYLEAAGQVGVT
                     DLGCAEHMYRFTEALTVWRHPFWEEWAIDDIDQYVEAVRDSPIKLGIEGDFIVGAEEK
                     LESMLGSRPFDYVVGSVHFLGDYSLDTEDYTIWDGSQDPDEIWRRYFETLASAARSGL
                     FDILAHPDLVKVWGRAGRLPSVDPRTMYEPAIEAIAETGIAVEVSTAGLRKGVEEIYP
                     APAFMEMCVEAG"
BASE COUNT          206 a          394 c          398 g          196 t
ORIGIN      
        1 ggtcaggcgg gccagttcga ggaaccggga atcatcacgg tggagggggg cgagacccgt
       61 tcccactcgg ttgccaacgg tctggcggcg atcaccgcgg acggtgatcc cggcatggtg
      121 gtcgttcacg atgccgcccg cccgctggtc agtccggccc tgatcgacgc cgccgtgatc
      181 ggcctggact ccgaccccga gctccacgcg ctgatcgccg cggctccgat gaccgacacg
      241 gtcaaacgcc tcggcgagga cggcatcgtg gtcgagaccc tcgatcgttc cgaactggtc
      301 tcggtgcaga cgccacaggt cttccggatc ccgaacctcc aggcggcaat cgcggccggg
      361 gacctttccg cggcgaccga cgacgcctcg ctggtcgagg cggtcggggg gtcggtgggc
      421 gtgatcgaat cgccttcagg caacatcaag gtcacggtcc ccgcggacct tgaactcgcc
      481 gctctcctcc tcgaaagaag ccgatgaccg acccagtgat aaccgactat cacctccacc
      541 tgcgcggtga cgaactctcg gcccgggcgg aagatcactt cacgaccgaa aacattgcgc
      601 gctacctcga agcggcgggg caggtcggag tcaccgacct cggctgtgcc gagcacatgt
      661 accgcttcac cgaggccttg accgtctggc ggcatccctt ctgggaggaa tgggcgatcg
      721 atgacatcga ccagtacgtc gaggcggtca gggacagccc gatcaaactc gggatcgagg
      781 gtgatttcat cgtcggggcg gaggagaagc tcgagtcgat gctcggctcg cgccccttcg
      841 actacgtggt cggatcggtt cacttcctcg gggactactc gcttgacacc gaggactaca
      901 cgatctggga cggcagccag gatcccgatg aaatctggcg ccgctacttc gagaccctcg
      961 ccagcgcggc ccgctccggg ctcttcgaca tcctggccca tcccgacctg gttaaggtct
     1021 ggggccgggc cggacgcctt ccctcggtcg atccgaggac catgtacgag ccggcgatcg
     1081 aagcgatcgc ggagaccgga atcgccgtcg aggtatcgac cgcgggcctg cgcaaggggg
     1141 tggaggagat ctaccctgcc ccggcgttca tggagatgtg cgtcgaggcg gggg
//
LOCUS       DAOQFI010000729         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_93950_length_1191_cov_2.905810, whole genome shotgun sequence.
ACCESSION   DAOQFI010000729 DAOQFI010000000
VERSION     DAOQFI010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93950_length_1191_cov_2.905810"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..719)
                     /locus_tag="PLE93_10945"
     CDS             complement(15..719)
                     /locus_tag="PLE93_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH87610.1"
                     /translation="MADGVWLLTGDIKGAMNIYFIEGPDGEIVQFDAATKSMVKANLR
                     VIREFGGIDRVVLGHAHADHRGTAPYLGVPVHCHPDEVADATSSASIAPYMYLKELEF
                     APVRWIYPFLLRRWDGGPVEIAGTVEEGDEIAGFKVIHFPGHAPGLIGLWRESDRVAL
                     VSDTVYFVDSARMRPLPEGEASVPHPAWAWDHQKAKESVRKLAGLGPKVVAAGHEHPL
                     RGEDLTDVLLRAADRH"
     gene            809..958
                     /locus_tag="PLE93_10950"
     CDS             809..958
                     /locus_tag="PLE93_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87611.1"
                     /translation="MPANWRILMIVICVFLVASVTIGAIQLLSTPTEIFGAGFEGLPK
                     QEFPR"
     gene            1011..>1191
                     /locus_tag="PLE93_10955"
     CDS             1011..>1191
                     /locus_tag="PLE93_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNH87612.1"
                     /translation="MSLFKRIVVGTDGSESAREAIRQAAELARMSGASLDVVSAFEPV
                     PDRRHQEEASGAPADV"
BASE COUNT          192 a          395 c          377 g          227 t
ORIGIN      
        1 cagtcgcccc ggagctagtg cctgtcggct gccctgagca gcacgtcggt caggtcctcg
       61 ccacggagcg ggtgctcgtg cccggcggcc accaccttcg gccccagtcc ggccagtttg
      121 cgcaccgact ccttggcctt ctgatggtcc caggcccagg ccggatgcgg taccgacgcc
      181 tcgccctccg gcaacggtct catccgggcc gaatccacga agtagacggt gtcgctgacc
      241 agggcgaccc ggtccgattc acgccacagt ccgatcaaac ccggggcatg cccggggaag
      301 tggatcacct tgaagcccgc gatctcgtcg ccttcctcga cggttccggc gatctcgacc
      361 ggaccgccat cccaacgccg cagcagaaag gggtagatcc agcggaccgg ggcaaactcg
      421 agttccttca ggtacatgta gggcgcgatc gaggcgctgg aagtggcatc cgcgacctcg
      481 tcgggatggc aatggaccgg cacgccgagg tagggggcgg tgccgcgatg gtcggcgtgc
      541 gcgtggccga gcacgaccct gtcgataccg ccgaattccc tgatcacccg caggttggcc
      601 ttgaccatcg atttcgtggc cgcgtcgaac tggacgatct ccccgtccgg gccttcgatg
      661 aagtagatgt tcatcgcgcc cttgatgtcg ccggtcagca gccagactcc gtccgccact
      721 tcctcgagtc ccttcggcgc tacccgattc gcgttcactt gctggcttcc ggtgtcatct
      781 tgagcgtctt ttccataacc tgaatcccgt gccggccaac tggagaattc tgatgatcgt
      841 gatctgcgta ttcctcgtgg cctcggtcac catcggcgca atccagttgc tttccactcc
      901 gaccgaaatc ttcggtgccg gttttgaggg tttgccaaag caggagtttc ccagatagga
      961 ctggtcccgg gggtaggatg ccggtgaccg cccacgacga tcggagacca atgtccctgt
     1021 tcaagcgcat agttgtcggt accgacggtt ccgagtcggc ccgcgaagcg atccgtcagg
     1081 cggccgaact ggcccggatg tcgggggctt cgctcgatgt cgtgagcgcc ttcgagccgg
     1141 ttccggaccg gcgtcaccag gaagaggcga gcggggcgcc ggctgacgtg g
//
LOCUS       DAOQFI010000730         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_94107_length_1190_cov_3.238767, whole genome shotgun sequence.
ACCESSION   DAOQFI010000730 DAOQFI010000000
VERSION     DAOQFI010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94107_length_1190_cov_3.238767"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLE93_10960"
     CDS             <1..497
                     /locus_tag="PLE93_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNH87613.1"
                     /translation="NQGRAIDMDWLDHDRLGFNYRMSDVTAAIGVAQLGRLDELLAGR
                     ARVAGLYLDRLAGVEGLELPAPDRPPAKRSWFVFPVRLPEGSDRDGVIRKLGEQGIPA
                     KAYLPSIHLFPHLAELGYREGQFPVAESVAARSLALPFHAGLSEGDIDRVASALADAL
                     GSGA"
     gene            506..>1190
                     /locus_tag="PLE93_10965"
     CDS             506..>1190
                     /locus_tag="PLE93_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNH87614.1"
                     /translation="MARHTDKSTSRFREPPDPRFWRLNRSIDFDRHLAPYDVAQSRAH
                     AAALNRIGVIADDEIKVIDEGLAQIADELKAGEFSFEQGDEDIHMAVERRLGELVGDL
                     AGKLHTGRSRNDQVATDICMAVMDAADGAGQRIATAMNGLLQLAENHRDWTMPGYTHL
                     QRAQPVYLGHHLLSWFWMLSRDFERFAAARRAAAVMPLGAGALAGVNWEIDRRRVAAD
                     LGFESVTINS"
BASE COUNT          185 a          407 c          401 g          197 t
ORIGIN      
        1 ggaaccaggg ccgggcgatc gacatggact ggctggacca cgaccggctc ggcttcaact
       61 accggatgtc cgatgtgacc gccgcaatcg gcgtcgccca gctcgggcgc ctggatgaac
      121 tgctggccgg ccgcgcccgg gtcgccgggc tctacctcga ccgcctggcc ggggtagagg
      181 gcctcgagct tcccgccccg gaccggccac cggcaaagcg cagctggttc gtgttcccgg
      241 ttcgacttcc cgaagggtcc gaccgtgacg gggtgatcag gaagctgggg gagcagggga
      301 ttcccgccaa ggcgtatctg ccctcgattc acctcttccc gcatctggcc gaactgggtt
      361 accgcgaagg ccagttcccg gtcgccgaat cggtcgcggc ccgttccctg gccctgcctt
      421 tccacgccgg gctgagcgag ggtgacatcg accgggttgc ctccgcactc gccgatgcgc
      481 tcggctcagg ggcataatca gcgccgtggc aagacacacc gacaagtcga cttcacgctt
      541 ccgcgagccg ccggatcccc gtttctggcg gctgaaccgg tcgatcgatt tcgaccgtca
      601 tctggcgcct tacgacgtcg cccagtcccg ggcccatgcc gcggccctga accggatcgg
      661 cgtgatcgcc gacgacgaga tcaaggtgat cgacgaggga ctggcacaga tcgccgatga
      721 actgaaggcc ggcgagttca gcttcgagca gggcgacgaa gacattcata tggcggttga
      781 gcgccgcctc ggcgagctgg tcggcgacct ggccggcaag ctccacaccg gtcggtcacg
      841 caacgaccag gtcgcgaccg acatctgcat ggccgtgatg gatgcggccg acggcgcggg
      901 gcagcggatc gcgactgcga tgaacgggct gcttcagctg gccgaaaatc accgcgactg
      961 gacgatgccc ggttataccc acctgcagcg ggcccagccg gtctatcttg gccaccacct
     1021 cctgtcctgg ttctggatgc tttcgcgcga tttcgagcgc ttcgccgccg cccgcagggc
     1081 tgcggcggtg atgccgctcg gggccggcgc cctggccggg gtcaactggg agatcgatcg
     1141 ccggcgggtt gcggccgacc tcggtttcga gtcggtcacg atcaactcgc
//
LOCUS       DAOQFI010000731         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_94126_length_1190_cov_2.772687, whole genome shotgun sequence.
ACCESSION   DAOQFI010000731 DAOQFI010000000
VERSION     DAOQFI010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94126_length_1190_cov_2.772687"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLE93_10970"
     CDS             <1..530
                     /locus_tag="PLE93_10970"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87615.1"
                     /translation="EQEATYASDGFASAYGNARFSAEARELPDPEPDAGILPSSSVPF
                     DQLVAFDAAHYFGPRPAFLRAWIKGEGRQSLVATDGTSITGFVASRPTRTGNRIGPFF
                     AANATIAGNLLLELATRLTGPVSVDAPEPNQAASDLFTSLGMKRSFPTIRMYRGLPPD
                     LPLDRIFGITTLELG"
     gene            587..>1190
                     /locus_tag="PLE93_10975"
     CDS             587..>1190
                     /locus_tag="PLE93_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87616.1"
                     /translation="MRAASVRENSRLRNLLREIGEIFAGIFHVYRAWWVDILVLSFCI
                     FVPIGLLDAADNQALESIGPGHDLTATALGIAALVITATALLGEVFLAGAVGLSLIHA
                     EDGRPPSLRFIARHLSYGRLIAVDILFVLAVALGLVFLVVPGMAAFAYLALAGPVVEI
                     EDRGIASSFRRSFQLVRGRFWLVFLILFPIEAVGGAVGQGS"
BASE COUNT          167 a          439 c          345 g          239 t
ORIGIN      
        1 cggaacagga agcgacctac gcctcggacg gtttcgcttc ggcgtatggc aacgccaggt
       61 tctcggcgga ggctcgcgag cttcccgatc cggaacctga cgccggcatc ctcccttcct
      121 cctcggttcc ctttgaccag ctggtcgcct ttgacgccgc tcattacttc ggccccagac
      181 cggccttcct tcgcgcatgg atcaagggag agggccgcca gagccttgtc gcaaccgacg
      241 gcacctcgat caccggcttc gtcgcctccc gcccgacccg caccggcaac cggatcggcc
      301 ccttctttgc cgccaacgca acgatcgccg gaaatcttct gcttgaactc gcgaccaggc
      361 tcaccggtcc ggtctcggtc gacgcccccg aacccaatca ggccgcttcc gacctcttca
      421 ccagcctggg gatgaaacgg agcttcccca ccatccgcat gtaccgcggc ctgccccccg
      481 accttcctct agaccgaatc ttcggcatca ccaccctcga actcggctga tcccttccga
      541 cccggtccga tcgcacgggg ccaacgtgat tgagagactt ctcacgatgc gcgccgcctc
      601 agtcagggag aacagccgct tgcggaacct gcttcgtgag atcggggaga tctttgccgg
      661 gatctttcat gtctaccggg cctggtgggt ggacatcctg gtgctttcgt tctgcatttt
      721 cgtcccgatc ggactgcttg acgcagccga caaccaggcg ctcgaatcga tcggcccggg
      781 tcacgacctg acggcgacgg ccctgggaat cgcggccctg gtcatcaccg ctacggccct
      841 gctcggcgag gtctttctcg ccggtgccgt cgggctctcg ctgatccatg cggaagacgg
      901 ccggccgccg tcgcttcgct tcatcgcccg ccacctcagc tacggccggc tgatcgccgt
      961 cgacatcctc ttcgtgctgg cggtggccct cgggctggtc tttctggtgg ttcccggcat
     1021 ggccgccttc gcctacctcg cgctggccgg gccggtggtc gaaatcgagg accgtggcat
     1081 cgcgagctcg ttcagacgca gcttccagct cgtccgcgga cgcttctggc tcgtcttcct
     1141 gatcctcttt ccgatcgagg cggtcggcgg cgccgtcggg cagggctctg
//
LOCUS       DAOQFI010000732         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_94182_length_1190_cov_2.116300, whole genome shotgun sequence.
ACCESSION   DAOQFI010000732 DAOQFI010000000
VERSION     DAOQFI010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94182_length_1190_cov_2.116300"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..693
                     /locus_tag="PLE93_10980"
     CDS             172..693
                     /locus_tag="PLE93_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87617.1"
                     /translation="MTQPTFQQFQRPLPGLSSHDRLLLFHALPESVQQSMWDRLAIQV
                     EDRRYADGDGPFIDRRPKPKRRPIRTASNLPSDSWLRGTEALASIPAETYLTVLAPES
                     EPTRGKCRCPLPDHEDHHPSATYKDTVWYCHVCGIGGTCFTIAAAKTGLNTRGRDFIE
                     LRKWIARQMLGAA"
     gene            690..>1190
                     /locus_tag="PLE93_10985"
     CDS             690..>1190
                     /locus_tag="PLE93_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH87618.1"
                     /translation="MIPLTPEQEAACLAEVQDSDPPRLLTLDQLADLPEPSYLVEDFI
                     RARGMTVLYGPSGGGKSFAALDWSLCIATGQPFYGQAVQQGPVVYIAAEGASGYYSRA
                     QAWCNSRGVAEVPDFRFWIEAPNFFRSETTALEVALAELDKPPVLIVIDTLARCLVGG
                     DENSARD"
BASE COUNT          233 a          422 c          342 g          193 t
ORIGIN      
        1 cctgaagccg aaggaagtcg ccgagctact cggcacaact acccgcaatg tcagcgccct
       61 ggcgagcaag ggacacctga ccagaatcgt cctcgggaac cgctcgaccc ggtacgaact
      121 cgaagacgtg cgccggttca tccgagatcg ccgaaacgtc gaggggccgg tttgacgcaa
      181 ccgaccttcc agcagttcca gcgtcctctt cccggtctgt cgagtcacga ccggctgctg
      241 ctctttcatg ctcttcccga aagcgtccag cagtcaatgt gggaccgcct cgccattcag
      301 gtcgaggacc gccgctatgc ggatggcgac ggcccgttca ttgaccggcg accgaaaccg
      361 aagcgtcgtc cgatccggac cgcgagcaac ctgccgagcg actcctggct aagaggcacc
      421 gaagcgctcg cctcgatccc ggccgagacg tacctgaccg ttctcgcccc cgaaagcgaa
      481 ccgacccgcg ggaagtgccg ctgtccgctg cctgaccatg aagaccacca cccatccgcc
      541 acctacaaag acacggtctg gtactgccac gtctgcggca ttggaggaac ttgcttcacc
      601 atcgcggcgg ccaagaccgg actcaacacg cggggccgtg atttcatcga actgcggaaa
      661 tggatcgccc gtcaaatgct tggggcggcg tgatcccgct gacccccgag caggaagccg
      721 cttgtctggc cgaagtccag gactcggatc ctccacggct gctcaccctc gaccagctcg
      781 ccgatcttcc cgagccgagc tacctggttg aggacttcat ccgggcgcga ggaatgaccg
      841 tcctctacgg gccgagcggc ggcggaaaga gcttcgccgc gctcgactgg agcctctgca
      901 tagctaccgg ccaacccttc tacggtcagg ccgtccagca gggtccagtc gtctacatcg
      961 cggccgaagg cgcttccggc tactacagcc gagcgcaggc atggtgcaac tcccgagggg
     1021 ttgccgaagt gccggacttc cgattctgga tcgaagcccc caacttcttc cggtccgaaa
     1081 cgacagccct cgaagtcgca ctcgccgaac tcgacaagcc cccggtcctg atcgtcatag
     1141 acaccctcgc ccggtgtctg gtcggcggcg acgaaaactc agcccgagac
//
LOCUS       DAOQFI010000733         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_94555_length_1187_cov_4.611307, whole genome shotgun sequence.
ACCESSION   DAOQFI010000733 DAOQFI010000000
VERSION     DAOQFI010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94555_length_1187_cov_4.611307"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLE93_10990"
     CDS             complement(<1..515)
                     /locus_tag="PLE93_10990"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNH87619.1"
                     /translation="MERALRDFAAEATPAEMTKALTQGEKERAICWGTLRELVADSAG
                     HKGNTMLSTEIEGWNPAFVDTGSDPEIDFLRMIRKREMPWPAVNVKLGHNVPDFLWWH
                     LRLAVELDPYGTHSGAGSHRRDHRKGIELETMGLRVVRFTGEDLYRHEERTGDELWIV
                     IRQQSRLLRCP"
BASE COUNT          218 a          372 c          348 g          249 t
ORIGIN      
        1 atcgggcatc gaaggagccg ggactgctgt cgaatgacaa tccagagttc gtcaccggtc
       61 ctttcttcat gccggtagag gtcttcgccc gtgaagcgga ctactcgcag gcccatggtt
      121 tcgagttcaa tccccttgcg atggtcgcga cggtgagatc ccgctccgct gtgcgttccg
      181 tacgggtcca gttcgaccgc cagccgtaga tgccaccaga ggaagtccgg gacgttgtgg
      241 cccagcttca cgttgaccgc gggccagggc atttcccgct tgcggatcat tcgaaggaag
      301 tcgatctccg gatcggagcc cgtatcgaca aaggctggat tccatccctc aatctcggtc
      361 gacagcatcg tgttcccctt gtgtccggcc gaatcggcca ccagctcgcg aagggtgccc
      421 caacaaattg cccgttcctt ctctccctgg gtcaatgcct ttgtcatttc ggccggagtt
      481 gcttcggccg cgaaatcgcg tagtgctcgc tccaccgtgg tcgtcggtat tccattcacg
      541 acggtgatct ggctgtctgg aagttgacgg gtttcgtgcc gtatgacctt gaagccgtag
      601 ttgtcgctgg accatctgcc ggatcgggtg ccgccagacc ctctcgaaac gccggtgagg
      661 tgaactaccc cgggatcgcg aacaacatcg tgaagtgcca acgcgctcga gtgcgagaca
      721 actgccccct cacctgccga acagaccccg gcgcgacacc ggcctcgaag tctcaaccgg
      781 gttcctggca gggcgaaaga ccccttgagg accggaatca accttccgga ctccttcctt
      841 gtctgaatca tcttgcgagt cagaccgatg gccgttaggt cttcggcagt cagaacaccg
      901 tcatgagtca gggcgacctc cctcatctgc cggttcagtt cactctcgat gcccgtccat
      961 ctgggatcca tccctggagc atgagcaaga aatgctcaca tgtggctcga caagagcaac
     1021 aattctgcaa cagaaaccgg ttcatgatgg ctgcggatcg gacccgcatc gggaggtgcc
     1081 gcaggtttcg ctccgtcgcg aggcgggagg tgccgcaggt ttcgccccat caaggggctg
     1141 gatttgcggc acctctgcca gtggtgaccc cgggggtggc cagcgac
//
LOCUS       DAOQFI010000734         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_94613_length_1187_cov_2.577739, whole genome shotgun sequence.
ACCESSION   DAOQFI010000734 DAOQFI010000000
VERSION     DAOQFI010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94613_length_1187_cov_2.577739"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLE93_10995"
     CDS             <1..336
                     /locus_tag="PLE93_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNH87620.1"
                     /translation="IERHKVVTPGELARIEGVQRPTMTRAISRLADAGLIDRREDPED
                     RRSALISVSQEGHRYLEAHRSRKSAWLANLIEEMPQEDADTLSRAAEILSHALESHKT
                     GEFSLPEDR"
     gene            333..>1187
                     /locus_tag="PLE93_11000"
     CDS             333..>1187
                     /locus_tag="PLE93_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH87621.1"
                     /translation="MTAALRRSFDSLQVPNFRRYFKGQVVSLSGNWMQTVAEVWLILS
                     LTGSGFAVGIATALQFLPILLLGAWGGSLADRFAKRDLLMVTQALMMIPPLIMLGVTA
                     AGVVTPAMIFALIFVRGTLLAVDNPARQAFVMDMVGPDRIVNAVSLNSVIVHSARVAG
                     PAVAGILIATVGVTPCFGLNAATFVVMIWALHGMDPKRLTEPPKSDREPGRVRAAIRH
                     VRNTPELKMPLLLMAVVGTLGLNFQVILPLLARLTFDGGASSYAVLVSSMGAGSVIGA
                     LITGARGKT"
BASE COUNT          190 a          407 c          370 g          220 t
ORIGIN      
        1 atcgagcgcc acaaggtcgt aactccaggt gaactcgccc ggatcgaggg cgtacagcgg
       61 ccgaccatga cccgggcaat cagccggctg gccgatgccg gtctgattga ccgccgtgaa
      121 gacccggagg accgtcgctc ggcgctgatc tcggtcagcc aggagggcca tcgctacctg
      181 gaggcccatc ggtcccgaaa gagcgcctgg ctcgcgaacc tgatcgagga aatgccacag
      241 gaggacgcgg acaccctttc ccgggcggcc gagatcctct ctcacgccct tgaatcgcac
      301 aagaccggcg agttctcgtt gccggaggac cggtgaccgc ggcgctgcgg cggtccttcg
      361 attcccttca ggtcccaaac ttccgccgct atttcaaggg acaggtcgtt tccctctcgg
      421 gaaactggat gcagaccgtc gccgaggtct ggctgatcct cagcctcacc ggctccggat
      481 tcgcggtcgg catagcaacc gcgctccagt tcctgccgat cctgctgctt ggagcgtggg
      541 gcggttctct cgccgatcgc ttcgccaagc gcgacctgct catggtcacc caggcgctga
      601 tgatgatccc gccactgatc atgctcggag tgaccgctgc cggcgtggtc acgcccgcca
      661 tgatcttcgc gctgatcttc gtccgcggaa ccctgctcgc ggtcgacaac cctgcccggc
      721 aggccttcgt catggatatg gtcggccccg accggatcgt caacgcggta agcctcaaca
      781 gcgtgatcgt tcattcggcc cgcgtcgccg gaccggccgt ggcgggcatc ctgatcgcta
      841 ccgtcggggt cacaccctgt ttcggtctga acgcggccac cttcgtggtc atgatctggg
      901 ccctccatgg catggatccg aagcgcttga ccgaaccccc gaagtccgac cgtgaaccgg
      961 gaagggtccg ggccgcgatc cggcacgtca gaaacacgcc ggaactcaag atgccactgc
     1021 tcctgatggc cgtggtcggg accctgggac tgaacttcca ggtgattctg ccgctgcttg
     1081 cccggctcac ttttgacggt ggggccagct cctacgcggt cctggtttcg tcgatgggtg
     1141 ccggttcggt gatcggcgcc ctgatcaccg gggcccgcgg caagacc
//
LOCUS       DAOQFI010000735         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_94622_length_1187_cov_2.520318, whole genome shotgun sequence.
ACCESSION   DAOQFI010000735 DAOQFI010000000
VERSION     DAOQFI010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94622_length_1187_cov_2.520318"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLE93_11005"
     CDS             <1..739
                     /locus_tag="PLE93_11005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HNH87622.1"
                     /translation="AEQFGTLESLNPGRIDLGIGRAPGTDQITAAALRRGVDPLSDED
                     LPRQLGELLGFFTGEWRSGHPYQAITAVPGEGAMPEIWLLGSSGYTAQVAGLLGMPYS
                     FAHHFSAGNTEAAVQLYRSRFEPSDFLEEPKVMIGVGVICAETDEEARWLAGPSRLAF
                     ARLRSGRPGRLPTPEEAEAHDFTPDEEAVVSSVARGHIFGSPETVRTGLVDLIERTGA
                     DELILSTMVYEQKDRLRSYELVADIEL"
     gene            850..1128
                     /locus_tag="PLE93_11010"
     CDS             850..1128
                     /locus_tag="PLE93_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87623.1"
                     /translation="MQTRLPQTSSGRARGFSLAAALIVAAFVIGLAFWARPADALTVD
                     QTQSKIDAINSSVDDLKAQVAEDNRSIDALIGELSGLRARANELEAQI"
BASE COUNT          203 a          419 c          365 g          200 t
ORIGIN      
        1 ggccgaacag ttcggcaccc tggagtcact caacccgggt cgcatcgacc tcggaatcgg
       61 ccgggcgccc gggaccgatc agatcaccgc ggccgcgctc cggcgcgggg tggaccccct
      121 ttccgacgag gacctacccc gtcagctagg cgagttgctc ggctttttca cgggggagtg
      181 gcgctccggc cacccctacc aggcaatcac cgcagttccc ggcgagggcg cgatgccgga
      241 gatctggctg ctcggctcca gcggctacac cgcccaggtc gcgggcctgc tcggcatgcc
      301 ctattccttc gcccatcact tcagtgccgg caacacggag gcggcggtcc agctttaccg
      361 ctcccggttc gagccctcgg acttcctcga agagccaaag gtgatgatcg gcgtcggggt
      421 gatctgtgcg gaaaccgatg aggaagcccg ctggctggcc ggaccctccc gactcgcctt
      481 cgcccggctc aggagcggcc ggccgggaag actcccgact cccgaagaag cggaggcgca
      541 cgatttcacc ccggatgagg aggcggtcgt ctcctccgtg gccaggggcc atatattcgg
      601 cagtcccgaa accgtgcgga ccggcctggt cgatctgatc gagcggaccg gagccgacga
      661 actgatcctc tcgaccatgg tctacgaaca gaaggaccgc ctcaggtcct atgaactggt
      721 cgcagacatc gagctgtaga gccagtttcc ttcaactgca cggtatttcc cacgaccccc
      781 ggacacgacg actgtcgacc ctgaggtcgc taccttgctc tttccgcccg cgctctgcgg
      841 cgggccctta tgcagaccag actcccgcaa acatccagcg gccgcgcccg cggcttcagc
      901 cttgctgcgg ccctgatcgt ggctgccttc gtgatcggtc tcgccttctg ggcgcgaccg
      961 gccgacgcgc tgaccgtcga tcagacccag tcgaagatcg acgcaatcaa cagctcggtc
     1021 gatgacctca aggcgcaggt agccgaggac aaccgcagca tcgacgcgct gatcggcgaa
     1081 ctttccggtc tgcgcgcccg cgcgaacgaa ctcgaggccc agatttgatc accggtttca
     1141 gcaggtcggc ggggcggcag tcgataatct cctccttacc gatcgcg
//
LOCUS       DAOQFI010000736         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_95113_length_1184_cov_2.314438, whole genome shotgun sequence.
ACCESSION   DAOQFI010000736 DAOQFI010000000
VERSION     DAOQFI010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95113_length_1184_cov_2.314438"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="rfbB"
                     /locus_tag="PLE93_11015"
     CDS             complement(<1..970)
                     /gene="rfbB"
                     /locus_tag="PLE93_11015"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNH87624.1"
                     /translation="MKLLVTGGAGFIGSNFVHHMVESGKGDGLVVLDKLTYAGRPENL
                     GGLDVELVEGDICDRDLVRGLVDRVDAVVNFAAESHVDRSIESPGEFITTDVFGTFVL
                     LEAARDAGIRHLQISTDEVYGSIDEGSFTEASPIVPSSPYSASKAGGDLIVGAFQHTY
                     GADTLIARASNNYGPQQHPEKLIPLAILNAIHDDPIPVYGDGSQVRNWLFVRDFCAAI
                     EQVLDQGEAGQVYNVGGPDELPNIQVIRTILELTGRDESLITHVGDRLGHDVRYSLAS
                     KKTEGLGWEAAVGFREGIGKTVDWYRENKWWWGPLRSGEYREYYQRQ"
     gene            complement(967..>1184)
                     /locus_tag="PLE93_11020"
     CDS             complement(967..>1184)
                     /locus_tag="PLE93_11020"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNH87625.1"
                     /translation="SWYDFAKEIFKLSEMEVTTLSATTEMFAAKAPRPAWSVLESGIE
                     HAITLPDWQDGLAAYLEQRRSLEGTGS"
BASE COUNT          217 a          371 c          387 g          209 t
ORIGIN      
        1 actgccgctg gtagtactcg cggtactcgc cggagcggag cgggccccac caccacttgt
       61 tctcccggta ccagtcgacc gtcttcccga tcccctcgcg gaagccgacc gcggcttccc
      121 agccgagacc ctcggtcttc ttcgaggcga gcgaataacg gacatcatgt ccgagccggt
      181 caccgacatg ggtgatcagc gactcgtccc tgccggtcag ctcgaggatg gtccggatca
      241 cctggatgtt cggaagctca tccggacccc cgacgttata gacctgaccg gcctcaccct
      301 ggtcgagcac ctgctcgatc gccgcgcaga agtcgcggac aaacagccag ttacggactt
      361 ggctgccgtc accgtaaacg gggatcggat catcgtggat cgcgttgaga atggccagcg
      421 ggatcagctt ctcgggatgc tgctgcgggc cgtaattgtt cgaggcccgg gcaatcaggg
      481 tgtcggctcc gtaggtgtgc tggaaagcgc cgacgatcag gtcgccaccg gccttcgagg
      541 ccgaatatgg cgaggagggg acgatcgggg aagcctcggt gaaggagccc tcgtcgatcg
      601 acccatagac ctcgtcggtc gagatctgga gatgacggat accggcgtcg cgagccgctt
      661 caagcagcac gaaggtgccg aagacatcgg tggtgatgaa ctcgccgggc gattcgatcg
      721 agcggtcgac atgggattcg gccgcgaagt tgaccaccgc gtccacccgg tcgaccagac
      781 cgcggaccag gtcccggtcg cagatgtcgc cttcgaccag ctcgacgtcg agccctccga
      841 gattctccgg ccggccggca taggtcagct tgtcgagcac gacgaggccg tcgcccttgc
      901 cggactcgac catgtgatga acgaaattgg agccgatgaa gccggccccg ccggtgacca
      961 gcagcttcac gagccagtcc cttcgaggct gcggcgctgc tcgaggtagg cggcaaggcc
     1021 gtcctgccag tcgggcaggg tgatcgcgtg ttcgatgccg ctttcaagca ccgaccaagc
     1081 tggtcggggc gccttggcgg cgaacatctc ggtggtcgca gaaagggtgg tgacttccat
     1141 ttccgacagt ttgaagattt ccttggcgaa gtcgtaccag gagc
//
LOCUS       DAOQFI010000737         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_96151_length_1177_cov_4.087344, whole genome shotgun sequence.
ACCESSION   DAOQFI010000737 DAOQFI010000000
VERSION     DAOQFI010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96151_length_1177_cov_4.087344"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLE93_11025"
     CDS             complement(<1..217)
                     /locus_tag="PLE93_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HNH87626.1"
                     /translation="MLSERQELILGLVVDAYRETGRPVGSKAIAGDSRLEWGASTVRA
                     ELAALEAGGYLTHPHTSAGRVPTDLGYR"
     gene            complement(263..658)
                     /locus_tag="PLE93_11030"
     CDS             complement(263..658)
                     /locus_tag="PLE93_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNH87627.1"
                     /translation="MSAKEKGEKIAGLKVTPDKFLPHRYAGASGDFNPIHIDKEFAQM
                     VGLPSTILHGLYVMGLVAKAATEEAGGDPRALKRLSVQFRGMSMPETEIEVSGEVIEA
                     DGADVTFDLIAAQGENQVIRNATADVTYS"
     gene            complement(655..1101)
                     /locus_tag="PLE93_11035"
     CDS             complement(655..1101)
                     /locus_tag="PLE93_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HNH87628.1"
                     /translation="MALKTDAVGKSWPAYEYEVGREKIREYAQVIGLDNPVHFDAEAA
                     RAAGYRGIVAPPMFVVVYAAGAMGPVLFDPDVEMNFAAMVHGGQEFEWGEPVISGDLI
                     TTTTTCRDIYPKDGKGFYVFETESKNQNGDLVCKATWTNIVRGIEE"
BASE COUNT          188 a          400 c          352 g          237 t
ORIGIN      
        1 gccggtagcc gaggtcggtc ggaacccgcc cggccgaagt gtggggatgg gtcagatagc
       61 cgcccgcctc aagcgcggca agctccgccc tgaccgtcga ggcaccccac tccagccggg
      121 aatccccggc aatcgccttc gaccccaccg gacgcccggt ttcccggtaa gcatccacaa
      181 ccagcccaag aatcagttcc tgtcgctcgg agagcatcac ttcattgtat gaagccgcgt
      241 tttccgcgac ctgcgagccg tcctacgagt aggtgacgtc ggcggtggcg ttccggatca
      301 cctggttctc gccctgggcc gcgatcaggt caaaggtgac gtcggcaccg tcggcctcga
      361 tcacttcgcc cgagacctcg atctccgttt ccggcatgct cataccgcgg aactggaccg
      421 aaagccgctt cagtgcccgc gggtctccgc cggcttcttc ggttgccgcc ttggcgacca
      481 gtcccatcac gtagaggcca tgcaggatgg tcgagggcag gccgaccatc tgggcgaatt
      541 ccttgtcgat gtggatcggg ttgaagtccc cggatgcccc ggcgtagcgg tgcggcagaa
      601 acttgtccgg agtgaccttg aggccggcga tcttctcgcc cttttccttc gcgctcattc
      661 ttcaatcccc ctgacgatgt tggtccaggt ggccttgcag acgaggtcac cgttctggtt
      721 tttcgactcg gtctcgaaga cgtagaaacc cttgccgtcc ttcgggtaga tgtccctgca
      781 ggtggtcgtg gttgtgatca ggtcgccgga gatcaccggt tccccccatt cgaattcctg
      841 gccgccgtgg accatcgcgg cgaagttcat ctccacgtcg gggtcgaata gcaccggacc
      901 catcgccccg gccgcataca cgacgacgaa catcggcggg gccactatcc cgcggtatcc
      961 cgccgccctg gcagcttcgg cgtcgaagtg gaccgggttg tcgagcccga tcacctgcgc
     1021 gtattcgcgg atcttttccc ggcctacctc gtactcgtag gccggccagc tcttgccaac
     1081 ggcatcggtc ttcagcgcca tgccggaaac ttacacccgg cccgggttca ccgagtgccc
     1141 ggtttgtgcc ggtatgcggc acaaagtggg atctcgc
//
LOCUS       DAOQFI010000738         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_96216_length_1177_cov_2.402852, whole genome shotgun sequence.
ACCESSION   DAOQFI010000738 DAOQFI010000000
VERSION     DAOQFI010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96216_length_1177_cov_2.402852"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1177
                     /locus_tag="PLE93_11040"
     CDS             29..>1177
                     /locus_tag="PLE93_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87629.1"
                     /translation="MVKVYSVRAKKDGLGPIELALTAPDRAAGEVALTYAVPAIGGYT
                     LLARISHSKDRYAFITPLGGARLDFEQVRKDYSGPRCQHCGLARARKTTFLLRGEDGE
                     IVQVGSTCLKEFLGDSDPKRALLQADLVSQARELIIAAAPAEKEDRTGRLPTVEEFLS
                     NVLAITEQEGGFVYKKNSGEDQTSTAELAIENFRMNQKNTFDPLGRPAWVEVRPEHVR
                     QTKEKIAEFTDLLSRQNEKHSDFDHRILQALEKDVAWPEKQGMLATVFERTKRLRQLE
                     TRLQGDGPWLGEVGETASVWATLVWVGKPFRAPSGGESIALHFSDTETGAAITCFQPA
                     GELPAWLAPRWNYPLSGTVRKHDEFKGQKVTVLDGWAIDLGGQGKMPRP"
BASE COUNT          252 a          344 c          363 g          218 t
ORIGIN      
        1 ccctgaccgc ctcgatcggc tcgtctcgat ggtcaaggtc tattccgttc gggccaagaa
       61 ggatggtctc ggaccgatcg agctggcgtt gaccgctccc gaccgcgcag ccggcgaggt
      121 cgcattgacc tacgcggtgc cggcgatagg tggctacacc cttctagccc ggatctccca
      181 ttcaaaagac cggtatgcgt tcatcacccc tttgggcggc gcccgattgg acttcgaaca
      241 ggttcgcaag gactattcgg ggccgcgctg tcagcattgt ggcctggcca gggcccgcaa
      301 gacaacgttc ctgttaaggg gcgaggacgg ggaaatagtc caggtcgggt cgacctgcct
      361 gaaagagttt ctcggtgaca gcgacccgaa acgggcgctt cttcaagccg acctggtctc
      421 gcaggcaagg gagctgatca ttgcggccgc accagctgaa aaggaagacc gaacgggacg
      481 tctccccaca gttgaagagt tcctttccaa tgtgcttgcg atcaccgagc aggaaggcgg
      541 attcgtctac aagaaaaact cgggcgaaga tcagacctcg actgcggaac tggcaatcga
      601 gaacttccgg atgaaccaga aaaacacgtt cgacccactg ggtcggccgg cctgggttga
      661 ggttcgcccg gagcatgttc gtcaaacgaa agagaagatc gctgagttca ccgatcttct
      721 ttcccggcaa aacgaaaagc acagcgattt cgatcaccgg atccttcaag ccctcgaaaa
      781 ggatgtggcc tggcctgaaa agcaggggat gctcgccacc gtgttcgagc gaaccaaacg
      841 gttgcgtcaa ctcgagacac gccttcaagg tgacggacca tggttgggcg aggtggggga
      901 gaccgcctcc gtgtgggcaa ccctagtttg ggttggcaaa ccgttccggg caccctccgg
      961 gggcgagagc atcgcccttc acttctccga caccgaaacc ggggcggcaa tcacctgttt
     1021 tcagccggcc ggggagctgc ctgcctggct tgccccccgc tggaactatc cgctgtccgg
     1081 aacggttcga aagcacgatg agttcaaggg ccagaaggtg actgttcttg acggctgggc
     1141 gatcgacctt ggtggccagg ggaagatgcc tagacca
//
LOCUS       DAOQFI010000739         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_96367_length_1176_cov_2.538805, whole genome shotgun sequence.
ACCESSION   DAOQFI010000739 DAOQFI010000000
VERSION     DAOQFI010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96367_length_1176_cov_2.538805"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLE93_11045"
     CDS             complement(<1..360)
                     /locus_tag="PLE93_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNH87630.1"
                     /translation="MSEKRDYYEILGVSREATDAEIKKAFRKVARELHPDVNAHDPEA
                     EEKFKEAAEAYEVLSDAERRGTYDQFGHDGLRSGGYQSQAQGFGSIDDLFSAFFGGGF
                     GASRGPAPGADVGVRTEI"
     gene            complement(357..>1176)
                     /locus_tag="PLE93_11050"
     CDS             complement(357..>1176)
                     /locus_tag="PLE93_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HNH87631.1"
                     /translation="GYRQYVESLKSRAVTRGAPAATLDLSDLRREVDGAMQEATSALA
                     QITDLLALVVAPPLGTARIHRVEALLLQPEVVMVIVITSTGDVTRRAFTFASPVDPGL
                     VEWASSYLNESLSGIDLGARRIASKLADPALGPLEANFIEQLAPALVDLEQGLEGTLY
                     VDGASRLLDQRRAADLPRIDRLMTTLEEKATVLRMMRSAIDERSVFLWIGAENPQPEL
                     QDVTVIGANYGLGHRNLGSVGVVGPTRMDYEKAIASVSEAARELSRYFEFVYDA"
BASE COUNT          189 a          391 c          399 g          197 t
ORIGIN      
        1 gatctcggtt cggaccccga cgtcggcacc cggcgccgga ccccgcgatg ctccgaagcc
       61 gccgccgaag aaggcgctga acaggtcgtc gatcgaaccg aacccctggg cctgcgactg
      121 gtagccaccg gagcgaagcc cgtcgtgacc gaactggtcg taggttcccc gccgctcggc
      181 gtcggagagc acctcgtagg cctcggccgc ctccttgaac ttctcttcgg cctcgggatc
      241 gtgagcgttc acgtccgggt ggagttcccg ggcgaccttg cggaatgcct tcttgatctc
      301 cgcgtcggtc gcttcccggc ttaccccgag aatctcgtag taatcgcgct tttcgctcat
      361 gcgtcgtaga cgaattcgaa gtagcgggaa agctcgcggg cggcttcact gacgctggcg
      421 atcgccttct cgtaatccat gcgggtcggg ccgaccaccc cgaccgagcc caggttccgg
      481 tggccgagcc cgtaattggc gccgatcacg gtcacgtcct gcagttcggg ctggggattc
      541 tcggccccga tccagaggaa gacggagcgc tcgtcgattg ccgagcgcat catcctgagc
      601 acggtcgcct tctcctccag tgtggtcatc aggcggtcga tccggggcag gtcggcagcc
      661 ctgcgctgat cgagcagccg ggaggcacca tcgacgtaga gcgtgccctc gagaccctgt
      721 tcgaggtcga ccagggccgg cgcaagctgc tcgatgaaat tggcctcaag cggaccgagc
      781 gccggatcgg caagtttcga ggcgatccgg cgagccccga gatcgatgcc ggagagcgac
      841 tcgttcaggt agctggaggc ccactcgacc agaccgggat cgactggcga ggcgaaggtg
      901 aaggcccgcc gggtgacgtc accggtcgat gtgatcacga tcaccatcac cacctcgggc
      961 tgcagcagca gggcttcgac ccggtggatc cgggccgtcc ccagtggcgg agccacgacc
     1021 agggcgagca ggtcggtgat ctgggccagg gccgaggtcg cttcctgcat cgccccgtcg
     1081 acctcgcgac gcaggtcgct gaggtcgagg gtggccgcgg gcgcaccgcg ggttaccgcg
     1141 cgggacttca gcgattcgac gtactgccgg tagccg
//
LOCUS       DAOQFI010000740         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_96468_length_1175_cov_4.026786, whole genome shotgun sequence.
ACCESSION   DAOQFI010000740 DAOQFI010000000
VERSION     DAOQFI010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96468_length_1175_cov_4.026786"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..766)
                     /locus_tag="PLE93_11055"
     CDS             complement(140..766)
                     /locus_tag="PLE93_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH87632.1"
                     /translation="MTRVGQAFDNPVTGERVVVLTDPFEHPDRVLAAHLTVAPGGRVA
                     MAHIHPAVRERFHVLGGQVGFLVGDEEVTLGPGESAEVPAGVVHDWWQIGEEEASVVV
                     EATPGDRLSEFITSIFGLCRDGKVNEDGVPRLLQIMVMVNEYSDEMILSSPPPAIQKT
                     IAAMLAPIGRLRGLKPSYPEYTTSTETVEPAPEALALLDEDGRLKGFA"
BASE COUNT          216 a          399 c          372 g          188 t
ORIGIN      
        1 cgagcagatc aagcgcttcc acatcctgcc ccatgacctg agccaggaag gcggcgagct
       61 gacgccgacc ctgaaaatca agcgcccggt cgtggcaacc aagtacgaga aggaaatcga
      121 agacctctac gccagctgac taggcgaacc ctttcaggcg accgtcctcg tcgagcagcg
      181 ccagagcctc cggcgcaggt tccacggttt cggtcgaggt cgtgtactcc gggtaggagg
      241 gtttgaggcc cctcaggcgg ccgatcgggg ccagcatcgc cgcgatcgtc ttctggatcg
      301 ccggcggcgg tgaggacaga atcatctcgt cgctgtactc gttgaccatg accatgatct
      361 gaaggagacg gggcacgcca tcctcgttca ccttgccgtc gcggcagagc ccgaagatgc
      421 tggtgatgaa ttcggagagg cgatcgcccg gcgtcgcctc gaccaccacg ctcgcctcct
      481 cttccccgat ctgccaccag tcgtgaacca cgccggccgg cacctcggcc gactcgcccg
      541 gaccgagggt cacttcttcg tccccgacca ggaacccgac ctgcccaccc agcacgtgga
      601 agcgctcgcg aacggccggg tggatgtgcg ccatcgcgac ccggccaccc ggcgccacgg
      661 tcagatgcgc ggccagaacc cggtcgggat gctcaaaggg atcggtgagc actaccaccc
      721 gttccccggt aaccggatta tcgaacgcct ggccaacccg agtcatgtcc agaacctatc
      781 cgcaacgggc ccccacggcg agagccacgc tgcccgttca ggtgaagttc atcgagatcg
      841 cattcatcgg cgctttacgc cgacaaaccg catctcgcga aaagaattca ccacttgcct
      901 cagcgggtcg tgcccaaggc gctcctgcgg gtggatgcca gggccttcag ccgtgctctg
      961 ccggggaggc atcgcccggg ttcgagtagc cgtggggtcg ggcaggcgtc tccgggacgc
     1021 tcaaacagag ggcggctgaa tgccgcccga ctgttgtccc ggtgatgcct gcccgatccc
     1081 gcggcgtgat ccaggagacc cgggctagga ggtgccggtt tggcggagga gagcgccggt
     1141 ggtctgctcg aagatgcgtc gccagaccga agctt
//
LOCUS       DAOQFI010000741         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_97131_length_1171_cov_3.658602, whole genome shotgun sequence.
ACCESSION   DAOQFI010000741 DAOQFI010000000
VERSION     DAOQFI010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97131_length_1171_cov_3.658602"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PLE93_11060"
     CDS             complement(<1..1154)
                     /locus_tag="PLE93_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87633.1"
                     /translation="MVPPASAGIAARLALDVKPGASGITSTGFSSYDQLTPIGDQLYF
                     TPYFARYGRELWRTDGRTLEMVRDIDPGPGYGYRSEQKPVEFQGRYYFQANSPGTGDE
                     LWRLSPDGSDAELVADIYPGPESGYPRALTVFKGQLYFAAGTPASGQELFRYDGTTVE
                     QFTDIKPGAGDAWAGFGKDTILDGYAYFLADDGVHGMELWRTDGENTELWLDATPGAE
                     GSYFFSLKAAGGRLYFATRTDYGLNTRAWRSDGVTNDVLGNFEIWQTTQVGQRVYLLS
                     GHSLMVVTGPDRQPEYKSLPFSAEVDNLTAIGNSLYFTATDRVHGSEPWRIKDGKLEL
                     LGSEAYDDQGSIGWYPGQTGPFVEFDGEAFITMSDSRYGSELWKVQDGKAV"
BASE COUNT          201 a          376 c          381 g          213 t
ORIGIN      
        1 accgccttgc cgtcctgcac cttccagagt tcactcccgt accgggagtc gctcatggtg
       61 atgaacgcct cgccgtcgaa ctcgacgaac ggccccgtct gccccgggta ccagccgatc
      121 gaaccctggt cgtcatacgc ctccgatccc aggagctcga gcttgccgtc cttgatccgc
      181 cagggctcgg acccgtggac gcggtcggtg gccgtgaagt agaggctgtt gccgatcgcg
      241 gtcaggttgt ccacctcggc gctgaaagga aggctcttgt actcgggctg acgatcgggt
      301 cccgtcacca ccatcaggct gtggcccgag aggaggtaga ccctttggcc aacctgggtc
      361 gtctgccaga tttcgaaatt gcccaggaca tcattggtca cgccgtccga gcgccacgcg
      421 cgggtattca gtccgtagtc ggtccgggtc gcgaagtaga gccggcctcc cgccgccttc
      481 aggctgaaga agtacgaacc ctcggctccc ggcgtcgcgt ccagccagag ctcggtgttc
      541 tcgccgtcgg tccgccagag ttccatgccg tgaacgccgt cgtcggccag gaagtacgcg
      601 taaccgtcga ggatcgtgtc cttgccgaac ccggcccagg catctcccgc gccgggcttg
      661 atgtcggtga actgctcgac ggtggtgccg tcgtaacgga aaagctcctg cccggaagcg
      721 ggggtgccgg ccgcgaaata gagctggccc ttgaagaccg tgagtgcgcg tggatatccg
      781 ctctccggac cgggatagat gtcagcgacc agctcggcat cggagccatc cggggagagt
      841 cgccagagct cgtcgccggt gccgggggaa ttcgcctgaa agtagtagcg gccctggaat
      901 tcgaccggtt tctgttccga tctgtagcca tatccgggtc cgggatcaat gtcccgaacc
      961 atctccaaag tccgaccatc ggtccgccac agctcgcggc cgtaccgggc gaagtagggg
     1021 gtgaagtaga gctggtcgcc gatcggcgtc agctggtcat acgagctgaa gccggtagac
     1081 gtgattccac tggcgccggg cttcacgtcg agcgccagcc ttgcggcgat tccggccgaa
     1141 gccgggggga ccatgatcag gcatgcgaag a
//
LOCUS       DAOQFI010000742         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_97164_length_1171_cov_2.801075, whole genome shotgun sequence.
ACCESSION   DAOQFI010000742 DAOQFI010000000
VERSION     DAOQFI010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97164_length_1171_cov_2.801075"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLE93_11065"
     CDS             complement(<1..359)
                     /locus_tag="PLE93_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid hydroxylase"
                     /protein_id="HNH87634.1"
                     /translation="MGSSERTERLAASPDLFENRLLNFFSRVHPVVPLLIYGPIIALL
                     VWLGVDRGLSALATVGLFVGGILIWTLSEYWLHRLLFHWTPKFRGGDKINFIIHGVHH
                     DHPNDKMRLVMPPAASIP"
     gene            417..>1171
                     /locus_tag="PLE93_11070"
     CDS             417..>1171
                     /locus_tag="PLE93_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNH87635.1"
                     /translation="MSETATATQDFKVADLSLADYGRKEIRLAEHEMPGLMATRREFA
                     DSKPLAGARITGSLHMTIQTAVLIETLVELGADVRWASCNIFSTQDHAAAAIAVGPNG
                     TPEDPQGVPVYAWKGETLEEYWWCTEQALTWPGTGADGSVGPNMILDDGGDATLLIHK
                     GVEFEKAGAVPDPAGADSEEFRIVLQTLTDSLAADSSKWTEIAKGIKGVTEETTTGVH
                     RLYEMLENGELLFPAINVNDSVTKSKFDNLYGCR"
BASE COUNT          242 a          371 c          380 g          178 t
ORIGIN      
        1 gggatgctgg cggccggggg catgaccagc cgcatcttgt cgttcggatg gtcgtggtga
       61 accccgtgaa tgatgaagtt gatcttgtcg ccgcccctga acttcggcgt ccagtggaaa
      121 agcaggcggt ggagccagta ctcggaaagg gtccagatca ggattccgcc gacgaaaagg
      181 ccgaccgtgg cgagggccga gaggccgcga tcgacgccga gccagaccag cagcgcgatg
      241 atcggcccgt aaatcagcag gggcacgacc gggtggaccc gggagaagaa attgagcagc
      301 cggttctcga agaggtcagg ggaagcggcg agcctctcgg tgcgttcgga cgatcccatt
      361 gcctgtggaa tggtagcgtg attggtcacc ctaactctca ggagaaaccc gcagtgatga
      421 gcgaaaccgc caccgcaaca caggacttca aggtcgccga tctttccctc gccgattacg
      481 gacgcaagga aatccgcctc gccgagcatg aaatgcccgg tctgatggcg acccgccgcg
      541 agtttgccga ctcgaagccg ctggcgggag cccggatcac cggctcgctc cacatgacca
      601 tccagaccgc cgtcctgatc gaaaccctgg tcgaactcgg cgccgatgtg cgctgggctt
      661 cctgcaacat cttctcgacc caggatcacg ctgccgccgc gatcgcggtc ggcccgaacg
      721 gcacccccga ggacccgcaa ggcgtcccgg tctacgcctg gaagggcgag accctggagg
      781 agtactggtg gtgcaccgag caggccctga cctggcccgg gacgggcgcc gacggttcgg
      841 ttggtccgaa catgattctc gatgacggcg gtgacgcgac cctgctgatc cacaagggag
      901 tcgagttcga gaaggccgga gccgtgccgg atccggccgg agccgactcc gaggaattcc
      961 gcatcgtcct gcagaccctg accgactcgc ttgccgcgga cagcagcaag tggaccgaaa
     1021 tcgcaaaggg catcaagggc gtgaccgaag agaccaccac cggggtccac cggctctacg
     1081 agatgctgga aaacggcgag ctcctcttcc cggcgatcaa cgtcaacgac tcggtcacca
     1141 agtccaagtt cgacaacctc tacggctgcc g
//
LOCUS       DAOQFI010000743         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_97483_length_1169_cov_2.832136, whole genome shotgun sequence.
ACCESSION   DAOQFI010000743 DAOQFI010000000
VERSION     DAOQFI010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97483_length_1169_cov_2.832136"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PLE93_11075"
     CDS             <1..973
                     /locus_tag="PLE93_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87636.1"
                     /translation="KETLVPILVGILFVLLAANVDMGQVIDLGWQGLLVIAALIVIAR
                     PLAALTLVGLPFTWREKVFFGSMAPRGIVAASTASAFGLELTEREVPGAEYVIPVTFL
                     IIIGTVFYYSLLTPWLAKVLGLAGEHRPTLMMIGAPPWALTIGEGLTQAGAEVLVWTE
                     SVEDAEAAARRGLVSFGGPLDPEEHEADSPLEDVAAFAVVSEDDTLNQLLSFQLAEIY
                     EANQVYRRRSPSDSSPIVRTEALELFHDGDTETEVQKRLSAGEPVSVVGPLARVPEGA
                     IPIAAVIPRRGTMPAKIHLATEDKPIKADGRSTLLVLNAVGSSPDGQ"
BASE COUNT          182 a          392 c          384 g          211 t
ORIGIN      
        1 caaggaaacg ctggtgccga tcctggtcgg gatcctcttc gtcctgctgg ccgcaaacgt
       61 ggacatgggt caggtgatcg accttggctg gcagggcctg ctggtcatcg ccgccctgat
      121 cgtcatcgcc cggccgctgg ctgcgctcac tttggtcggg ctgcccttca cctggcggga
      181 aaaggtcttc ttcgggtcaa tggctccgcg cggcatcgtc gccgcttcaa ccgcctccgc
      241 cttcggcctc gagctgaccg agcgcgaagt gccgggggcc gaatacgtca ttccggtgac
      301 cttcctgatc atcatcggca cggtcttcta ctactcgctg ctgaccccct ggctggccaa
      361 ggtcctgggt ctcgccgggg agcaccggcc gaccctgatg atgatcgggg caccgccgtg
      421 ggcgctgacc ataggggagg gcctgaccca ggccggagcc gaagtgctgg tctggaccga
      481 atcggttgag gacgccgagg ccgcggcccg gcgcgggctc gtttccttcg gtggaccgct
      541 cgatcccgag gagcatgagg cggattcccc gctggaggat gtcgccgctt tcgccgtggt
      601 cagcgaggat gacacgctga accagcttct gtccttccag ctggcggaaa tctacgaagc
      661 caaccaggtc taccgccggc gatcaccttc cgacagctcg ccgatcgtcc gcaccgaggc
      721 gcttgaactg ttccacgacg gcgacaccga gaccgaggtc cagaagaggc tttcggccgg
      781 cgagccggtt tcggtggtcg gcccgctggc ccgggttccc gagggtgcga ttccgatcgc
      841 cgccgtcatc ccccgccgcg gaacgatgcc ggccaagatt cacctggcga ccgaggacaa
      901 accgatcaag gcggacggcc gctcaacgct cctcgtcctt aacgccgtcg ggtcttcgcc
      961 cgacggccaa tgaaacccgc ccgcccacag agcaaaagaa tggatgggcg ggctccggtt
     1021 ttctagccgc gccagcgtcg ttggaagggg aggcgccagc ggtttggttc gatcaggtcg
     1081 tggatttccc gaggacccca ggaaccgcgt tcgtaggtca gcaccccggg cgggttctca
     1141 tcgacggcgt cgggaccggc cttgatctt
//
LOCUS       DAOQFI010000744         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_97622_length_1168_cov_3.233603, whole genome shotgun sequence.
ACCESSION   DAOQFI010000744 DAOQFI010000000
VERSION     DAOQFI010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97622_length_1168_cov_3.233603"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLE93_11080"
     CDS             complement(<1..223)
                     /locus_tag="PLE93_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87637.1"
                     /translation="MIALILLASATALATGLGAIPVMLLGNRARSIVPLLLGIAGGVM
                     AVASVMGLIIPALDRGTVAEVVGGLAIGTG"
     gene            305..685
                     /locus_tag="PLE93_11085"
     CDS             305..685
                     /locus_tag="PLE93_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH87638.1"
                     /translation="MTEIIAPKVGKNSPRQAWLLDFNLAFVGGDIEQTLGFVAEDVTW
                     ELVGEGTIKGRDGMRAWLQSMAGKQARRVEFRLFITHGRSAAVNGSYEMESGSKFEFC
                     DIYEFANAKNDSPITKYTSYVVRV"
     gene            703..>1168
                     /locus_tag="PLE93_11090"
     CDS             703..>1168
                     /locus_tag="PLE93_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87639.1"
                     /translation="MELKPKQSDKPGKERGNDFERVHGDLLRSFLEPGESLLGVAAVN
                     WQRSMFKQTVSALGVTGSRLIIQPLDRKGKAATEAPTFIRKEEITKGSYGGGGGMGNS
                     PTSLIMDSASIEVKLKTSGGEKYKLLLMTGEGMFGSMAGGPAQRNGAEALVSF"
BASE COUNT          237 a          349 c          413 g          169 t
ORIGIN      
        1 aaccggtccc gatcgccagg cccccgacga cctcggctac ggtgccgcgg tcgagggccg
       61 ggatgatcag ccccattacg gaagccacgg ccattacccc tccggcgatg ccgagcagca
      121 ggggcacgat ggaccgggcc cggttgccga gcagcatcac cgggatcgcc ccgagtccgg
      181 tggcgagggc ggtggcgctg gcgaggagaa tcagtgcgat caagacgact caaagcgtag
      241 gggcgacggc ggaggcggtg accggccctt cgccgagcac tccttcagcg actaggctgg
      301 ccgcatgacc gagatcatcg cgccgaaggt cgggaagaac tcgccgcgtc aggcctggct
      361 gcttgatttc aacctcgcct tcgtcggcgg ggatatcgag cagacccttg gcttcgtggc
      421 cgaggacgtc acctgggagc tggtcggcga ggggacgatc aagggtcgcg acggaatgcg
      481 ggcctggctt cagtcgatgg ccggcaagca ggcccggcgg gtcgagttca ggctcttcat
      541 cacccacggc cgatctgccg cggtcaacgg cagctacgag atggaaagcg gttcgaagtt
      601 cgagttttgc gacatctacg aattcgccaa tgcgaagaac gacagcccga tcacgaaata
      661 cacctcgtat gtagtgcgag tctgaaagga gcggggcggc gcatggaact caaaccgaag
      721 cagtccgaca agccgggaaa ggagcgggga aacgacttcg agcgggtcca cggggatctg
      781 ctcaggtcct tcctcgagcc gggcgagagc ctgctcgggg tggcggcggt caactggcag
      841 cgcagcatgt tcaagcagac ggtcagcgcg ctgggggtga ccgggagcag gctgatcatc
      901 cagccgcttg accgcaaggg caaggcggcc acggaggccc cgactttcat ccgcaaggag
      961 gagatcacca agggcagcta cgggggaggt ggcggcatgg gcaactcgcc gacctctctg
     1021 atcatggact ccgcatcgat cgaggtgaag ctgaagacct ctggcggcga gaagtacaag
     1081 ctgctcctga tgaccggcga aggcatgttc ggcagcatgg ccggcgggcc ggcacaaaga
     1141 aacggcgccg aagccctggt cagcttcc
//
LOCUS       DAOQFI010000745         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_97913_length_1166_cov_3.235824, whole genome shotgun sequence.
ACCESSION   DAOQFI010000745 DAOQFI010000000
VERSION     DAOQFI010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97913_length_1166_cov_3.235824"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLE93_11095"
     CDS             complement(<1..233)
                     /locus_tag="PLE93_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87640.1"
                     /translation="MLLERTHGVRDYFGGRIADQDPIQRAANATGYGQASTVAAALEA
                     LIAQGESSLTPMELEALVAQCTGRGAPNFAMQA"
     gene            complement(342..>1166)
                     /locus_tag="PLE93_11100"
     CDS             complement(342..>1166)
                     /locus_tag="PLE93_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87641.1"
                     /translation="WIEASPPTFFHRPDMMLATAGAAVGLVAAAWRLAGGRLNLPQGE
                     RARGVWLAVAMVASVFICTLVLLALGTDIFGARNLAPAWVGMPFLFGSVFVAAGRRAG
                     WVFLILMIAGFMVSTTRLMDPNRSTINYSRVADQLDDSGEKGTILDASIASPAPLTSL
                     DAYLETDLPVFKMTAIEDRPDYIKQLFATYDAQKILDRAFSGAGPVRVVTADDFRAPR
                     EITSGGAAGGYEIQVNGQTAVIPPGWEPVSSHRYPGLEPLTVTVFERSGQTGKSDR"
BASE COUNT          196 a          382 c          400 g          188 t
ORIGIN      
        1 tgagcctgca tcgcgaagtt cggcgcgccg cggcccgtac actgagcaac cagcgcctca
       61 agctccatgg gagtaagcga ggattccccc tgggcaatca aggcctcgag cgccgcggcc
      121 accgtgctgg cctgtccgta gcccgtcgcg ttggccgccc tttgaatggg atcctgatcg
      181 gcaattcgac cgccgaaata gtcgcgaacc ccgtgcgttc gttcgagtag caccgtgagc
      241 ggcgcacctt gtggtgggct gtaccgggga tgctcgatcc agaacgcgag ggcgttgcgg
      301 aaatcgccgg cccgctccca gccatttctc gatccgctcg ttcaccggtc gctctttccg
      361 gtttggccgc ttcgctcgaa gaccgtgacg gtgagcggtt ccaggcccgg gtaccggtgg
      421 ctggagacgg gttcccagcc gggcgggata acggctgtct gtccgttgac ctggatttcg
      481 tatccgccag ccgccccgcc gctcgtgatc tcccggggcg cccggaagtc atcggcggtc
      541 accacccgca ccggacccgc gccgctgaag gcacggtcga ggatcttctg ggcgtcgtag
      601 gtggcgaaga gctgcttgat gtaatcgggg cggtcctcga tcgcggtcat cttgaagacc
      661 ggcaggtcgg tttccagata ggcgtcgagc gaggtcaggg gcgcggggct ggcgatcgag
      721 gcgtcgagga tcgtcccctt ctcacccgag tcgtcgagct ggtcggcgac ccgggaataa
      781 ttgatcgtcg agcggttcgg gtccatcagg cgagtggtcg agaccatgaa cccggcgatc
      841 atcaggatca aaaagaccca gccggcccgg cggccggcgg cgacgaagac cgaaccgaag
      901 aggaatggca tgccgaccca ggccggagcc aggttgcggg ctccgaagat gtcggttccg
      961 agggcgagca ggaccagagt gcagatgaag accgaggcca ccatcgccac cgccagccag
     1021 actccgcgcg ccctttcgcc ctgcggcaga ttcagtcggc caccagccag gcgccaggcc
     1081 gcagcgacca gcccgaccgc cgccccggcc gtggcgagca tcatgtccgg ccggtggaag
     1141 aaggtcgggg ggctggcctc gatcca
//
LOCUS       DAOQFI010000746         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_98480_length_1163_cov_2.385379, whole genome shotgun sequence.
ACCESSION   DAOQFI010000746 DAOQFI010000000
VERSION     DAOQFI010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98480_length_1163_cov_2.385379"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLE93_11105"
     CDS             complement(<1..816)
                     /locus_tag="PLE93_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH87642.1"
                     /translation="MDSMELRGKVVLITGGARGIGFETARLAREQGASVALIDLDPEI
                     VSESALALGGDTIGLAADVTDLGAMEAAVAQTVERLGGIDAVIASAGITPPKTTARAI
                     DPTVWEKVVEVNMVGVYNTARAAVEPIISSRGHMVLIASVAAQANGALNTSYAASKAG
                     VESLGRSLRAELEPHGASAGVAYFGYVETDLISEVFGNELADSFRREIAPDFLTRTMP
                     VSQVSGALIEGIRSRAPRIIEPPAWRPILGMRGAVGPFSDRALERNPKVGEFIR"
     gene            913..>1163
                     /locus_tag="PLE93_11110"
     CDS             913..>1163
                     /locus_tag="PLE93_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87643.1"
                     /translation="MASTELTSGARTLIPFRRLAAATAFATFALIVVGGIVRVSESGL
                     GCGPAGSGLEGWPLCTGRVVPSVGGATLVEFTHRVLAGLV"
BASE COUNT          191 a          400 c          376 g          196 t
ORIGIN      
        1 ccggatgaac tcgccgactt tcgggttccg ctcaagggcg cggtcgctga acgggcccac
       61 cgctccccgc atgccgagga tcggacgcca ggcgggcggt tcgatgatcc gcggcgcccg
      121 ggaacggatg ccctcgatca gggctccgga cacctggctg accggcatcg tccgggtcag
      181 gaagtccgga gcaatctccc gtcgaaagct gtcggcgagc tcattgccga agacttcgct
      241 gatcaggtcg gtctccacgt aaccgaagta ggcaaccccg gcagaagctc cgtgcggttc
      301 gagttcggcc ctcagtgaac ggccgaggga ttccaccccg gccttcgagg cggcgtagga
      361 agtgttgagg gccccgttcg cctgggcggc tactgacgcg atcagcacca tgtgaccccg
      421 actcgagatg atcggctcca ccgcggcccg ggccgtgttg tacacgccga ccatgttgac
      481 ctcgacgacc ttctcccaga ccgtcggatc gatcgcccgc gccgtcgttt tcggcggagt
      541 gatgccggcg ctcgcgatca ccgcgtcgat accgcccagc cgttcaacgg tctgggcgac
      601 ggctgcttcc atcgcgccca gatcggtcac gtcggcggcc aggccgatcg tgtccccgcc
      661 cagcgcgaga gcagactcgg aaacgatttc cggatcgaga tcgatcaaag cgacggaagc
      721 tccctgctcc cgggccagcc gcgccgtctc gaaaccgatt ccccgggcac caccggtgat
      781 cagcacgact ttgcccctca gctccatcga atccatcaca ccactctaga gcctgtttgc
      841 cggtggaaaa ccgcaggctg gatgtggtta aaccgcaatc ccgaggagat tctgcgaact
      901 atcctcggcg ggatggccag cacggagctg acatccgggg ccagaaccct gatccctttc
      961 aggcggctcg cggccgcgac cgcattcgcc accttcgcgc tgatcgtggt cggtggcatc
     1021 gtcagggtga gcgagtcggg actgggctgc gggccggccg gcagcggact cgagggatgg
     1081 ccactctgta ccggcagggt ggtcccttcg gtcgggggcg cgaccctggt cgagttcacc
     1141 caccgcgtgc tcgccggcct ggt
//
LOCUS       DAOQFI010000747         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_98762_length_1161_cov_3.169982, whole genome shotgun sequence.
ACCESSION   DAOQFI010000747 DAOQFI010000000
VERSION     DAOQFI010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98762_length_1161_cov_3.169982"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLE93_11115"
     CDS             <1..678
                     /locus_tag="PLE93_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
                     /protein_id="HNH87644.1"
                     /translation="VPSMAMAHQGNPDYRSEITSVQPEALGEGLTIEIVNFDDHVRLV
                     NKTGKVVLIKGYDGEPYARVEADGKVFVNLNSPAYYLNEDRFAAVEVPKRADAKAAPD
                     WKQIADNGTFEWHDHRSHYMGTGTPSQVTDESKETKVFDYVIPIEVGGEPAKINGTLT
                     WVGTGSKAPVIPFVILGLAIIAAIVFWLMRRRRSEDDDGPREGGSGEAGGSGDDNPDE
                     SGKSEAW"
     gene            675..>1161
                     /locus_tag="PLE93_11120"
     CDS             675..>1161
                     /locus_tag="PLE93_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNH87645.1"
                     /translation="MRRSLLTSLLFLACLLGLASSASAHAVLEGSSPTRGAELNSSPG
                     HVDFTFNEPVEASLGAVRVFNTSGDQVQTDDLERPGENADAVGASLPADLPDGLYTAT
                     YHVISADSHPVSGGITFTIGKPGKDASAFTQGKTISELLAESEAGKVTEGAFWADRWI
                     GY"
BASE COUNT          223 a          365 c          375 g          198 t
ORIGIN      
        1 gttccttcga tggcgatggc ccaccagggc aatcccgact accggtccga gatcacctcg
       61 gtccagccgg aagcgctggg cgagggactc acgatcgaga tcgtgaactt cgatgaccat
      121 gttcgcctgg tcaacaagac cggaaaggtt gttctgatca agggatatga cggtgagcct
      181 tacgcccggg tcgaggccga cggcaaggtt ttcgtgaacc tgaattcgcc ggcctactac
      241 ctcaacgagg accggttcgc ggcggtggaa gtgccgaagc gggccgatgc gaaagccgct
      301 cccgactgga agcagatcgc cgacaacggc accttcgagt ggcatgacca ccgctcgcat
      361 tacatgggca cgggaacgcc ctcgcaggtg accgacgagt cgaaggagac caaggtcttc
      421 gactatgtga tcccgatcga ggtcggtggc gagccggcga agatcaacgg caccctgacc
      481 tgggtcggta ccggatcgaa agcgccggtg atccccttcg tgatcctcgg gctggcgatc
      541 atcgccgcga tcgtcttctg gttgatgcgg cgacgccgca gcgaagatga tgacggacct
      601 cgcgaaggcg gatccgggga agcaggtgga tcgggcgatg acaacccgga tgaatcaggc
      661 aagagtgagg cctggtgagg cgcagcctcc tcacaagtct gcttttcctg gcctgcctgc
      721 tcgggctggc cagttcggcc tctgcccatg ccgtgctcga gggttcatcc ccgactcggg
      781 gggcggagct caactcctca cccggccacg tggacttcac cttcaacgag ccggtcgaag
      841 cgtcgctggg tgcggtccgg gtcttcaaca catccggtga ccaggtccag accgatgacc
      901 tcgagcgtcc gggtgaaaac gcggacgcgg tcggagccag cctgcccgcg gacctgcccg
      961 acgggcttta caccgcgacc taccatgtga tctcggccga ttcccacccg gtttcgggcg
     1021 gcatcacttt cacgatcggc aaacccggca aggacgcatc cgccttcacc cagggcaaga
     1081 cgatttcgga actcctggcc gaaagcgagg cgggcaaggt caccgagggt gccttctggg
     1141 ccgatcgctg gatcggctac c
//
LOCUS       DAOQFI010000748         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_99211_length_1159_cov_1.637681, whole genome shotgun sequence.
ACCESSION   DAOQFI010000748 DAOQFI010000000
VERSION     DAOQFI010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99211_length_1159_cov_1.637681"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="rfbD"
                     /locus_tag="PLE93_11125"
     CDS             complement(<1..665)
                     /gene="rfbD"
                     /locus_tag="PLE93_11125"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNH87646.1"
                     /translation="MKITVLGARGMLGNDVMLAAENEGHEVHGYGHAEVDITDPDSVA
                     RRLRLDRPDAVVNCAAWTDVDGAEDEAEGALAVNGTGAGIVAEAAAAVDAKVLYVSSD
                     YVFDGSKTTPYVETDQTGPVSSYGVSKLAGEVATLNANRRCFVVRSSWLFGINGGNFV
                     DTMIRLGKTQKQVLVVRDQVGCPTYTWHLAYGLVRLLDSDAYGIHHMAGGGNCSWYDF
                     AKE"
     gene            complement(665..>1159)
                     /locus_tag="PLE93_11130"
     CDS             complement(665..>1159)
                     /locus_tag="PLE93_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HNH87647.1"
                     /translation="GELEITEAIQTLIDDGHDVRSEVVSGWWKDTGQLVDMLEANRLV
                     LDDIGSRLDGTIEDSRVEGRVIVEEGAVLKNSVVRGPAVIGAGAVIESAYIGPYTSIG
                     DGVRVVNSEIEHSILLSGSSVENLGARIEASLLGREVRVTRSDSLPKTMRLVDGDRAE
                     IEIV"
BASE COUNT          199 a          401 c          351 g          208 t
ORIGIN      
        1 atttccttgg cgaagtcgta ccaggagcag ttcccgcccc cggccatgtg atggatgccg
       61 taggcatcgg agtcgagcag acggaccagc ccataggcga gatgccaggt gtaggtcggg
      121 cagccgacct gatcgcggac caccagcacc tgcttctggg tcttgccgag ccggatcata
      181 gtgtcgacga agttgccacc gttgatgccg aaaagccagg aggagcggac cacgaaacag
      241 cgccggttcg cgttcagggt cgcgacctcg ccggccagtt tcgagacgcc gtaggacgag
      301 accggaccgg tctgatcggt ctcgacatag ggcgtggtct tgctgccgtc gaagacatag
      361 tcgctcgaca catagagcac cttcgcgtca accgccgccg ctgcttccgc gacgatcccg
      421 gcgccggttc cgttgaccgc aagcgcgcct tcggcctcgt cttcggcccc gtcgacatca
      481 gtccaggcgg cgcagttgac gaccgcgtcg gggcgatcga gccggagccg tcgggcgacc
      541 gaatcggggt cggtgatgtc aacctccgcg tgaccgtagc cgtggacctc gtggccttcg
      601 ttttccgcgg ccagcatcac atcgttgccc agcatgccgc gggctccgag cacggtgatc
      661 ttcatcagac gatctcgatc tcggcccggt cgccgtccac cagccgcatc gtcttcggca
      721 gggaatcgct gcgggtgacc cggacctccc ggcccagcag gctcgcctcg atccgggcgc
      781 ccaggttctc gaccgaggaa ccggagagca ggattgaatg ctcgatctcg gagttgacga
      841 cccggacccc gtccccgatc gaggtgtagg ggccgatgta ggcactttcg atcactgctc
      901 cggcaccgat caccgccggc cccctgacca ccgagttctt cagcacggca ccctcttcga
      961 cgatcacccg gccttcaacc cgcgaatcct cgatggtccc atcgagccgg ctgccgatgt
     1021 catcgagtac cagccggttg gcttccaaca tgtcgaccag ctgaccggtg tccttccacc
     1081 agccgctgac cacttcggaa cgaacatcgt ggccatcgtc aatcagtgtc tggatcgcct
     1141 cggtgatctc cagctcgcc
//
LOCUS       DAOQFI010000749         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_99668_length_1156_cov_2.008174, whole genome shotgun sequence.
ACCESSION   DAOQFI010000749 DAOQFI010000000
VERSION     DAOQFI010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99668_length_1156_cov_2.008174"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLE93_11135"
     CDS             complement(<1..542)
                     /locus_tag="PLE93_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNH87648.1"
                     /translation="MHLIVAEKNISAHRIASILAGKTKVATDKDGGVPVYRFGDTAVI
                     GLRGHVVEIDFEEGYSNWRSTERTPRSLIDARTIKVPTEKKIVQVLQKLAKKSSLITI
                     ATDFDTEGELIGKEAYELIRQVSREVPVRRARFSAITPDEIRQAFSGTSELDFDLASA
                     GEARQVIDLMWGASLTRFISL"
     gene            complement(548..>1156)
                     /locus_tag="PLE93_11140"
     CDS             complement(548..>1156)
                     /locus_tag="PLE93_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87649.1"
                     /translation="AQRLGRAAHVRDVDEGLRAHRRADHRRRAAGDEAEGDLRVAEFY
                     EQVAGIDPVFVATWVADTLLGELNYRDMSINRVSPDWFRELIVLVHKGNVTDKGGIEV
                     LRSLLDQIRESGSCETPGVCVGRLGLRVLERTAETMSEDDPVVKAAREVIAEQPQAVA
                     DFAAGKKEAFNFLVGKVMKKTRGRAKPADVNQVLLAILGKEG"
BASE COUNT          200 a          368 c          343 g          245 t
ORIGIN      
        1 aggctgatga accgggtgag ggaagccccc cacatcaggt cgatcacctg ccgggcttcc
       61 cctgcggagg cgaggtcaaa atcgagttcc gaggttccgc tgaatgcctg cctgatctca
      121 tcgggggtga tagcactgaa ccgggcccgc cggacaggaa cctcccggct cacctggcgg
      181 atcagttcgt acgcctcctt cccgatcagc tccccttcgg tgtcgaaatc ggttgcgatc
      241 gtgatgaggc tggacttctt tgccagtttc tggaggacct ggacgatctt cttctcggtc
      301 gggaccttga tcgtccgggc gtcgatcagg gaccgggggg tccgttcggt gctccgccag
      361 ttggagtaac cctcttcaaa atcgatctcg accacgtggc ctctgagccc gatgaccgcg
      421 gtatccccga accggtagac cgggaccccc ccatccttgt cggtggccac tttggtttta
      481 ccggcaagga tggaggcgat gcggtgagcc gagatattct tctcggcaac gatcaggtgc
      541 atcgcgttca gccctctttt ccgaggattg ccaacaatac ctgattcaca tcagcgggct
      601 tggcacggcc cctggtcttt ttcatgactt tcccgacgag gaaattgaac gcttccttct
      661 ttccggccgc aaaatcggcg actgcctggg gttgctcggc aatcacttcc cgtgccgcct
      721 tcaccactgg gtcgtcttcc gacatggttt cggcggtccg ttccaggacc cggaggccga
      781 gtctcccgac acaaacgccc ggggtttcac aggatcccga ctccctgatc tggtcgagga
      841 gggatcggag cacttcgatc ccacctttat cggtcacgtt ccctttgtgg acgaggacta
      901 tcagctcccg gaaccagtcg ggggagaccc tgttgatact catgtcccgg tagttgagtt
      961 caccaagaag ggtatcggca acccaggtcg caacgaacac cgggtcgatt ccggcaacct
     1021 gctcgtagaa ttccgccacc cggagatcac cttccgcttc gtcgccggcg gcgcggcgcc
     1081 ggtgatcggc gcgacggtgg gcacgcagac cttcgtcaac gtcacgcacg tgggcggcac
     1141 ggccgaggcg ctgggc
//
LOCUS       DAOQFI010000750         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_99685_length_1156_cov_1.851953, whole genome shotgun sequence.
ACCESSION   DAOQFI010000750 DAOQFI010000000
VERSION     DAOQFI010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99685_length_1156_cov_1.851953"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLE93_11145"
     CDS             <1..775
                     /locus_tag="PLE93_11145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH87650.1"
                     /translation="YLLPYFLLMMIGLSPIEAGIALIPATVPIMLAGPLAGRLFDRVG
                     GRIPLVIGFLVLGASGIAFALAVSEREYVYLLPGLILQGIGLGMVLTVNDPTGLDSVP
                     PEDQGQAAGVINTSEQLGGAVGIALFSAVLINTSIHRTNESLAAKGIHPTARQTAEWK
                     EFIQTINSKGLGHIDISKQSETIRFALNDVLSAHVAGYEAVFYSTAGIALLGALACFF
                     LVRKTDRVEQGRVFVRRSRWVSSNTGRTPAVTKHPPRPK"
     gene            complement(804..>1156)
                     /locus_tag="PLE93_11150"
     CDS             complement(804..>1156)
                     /locus_tag="PLE93_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87651.1"
                     /translation="IPIVGQVRGTGHFWAIEVVRDQETKEPFKGEAAEELFKNVLSEA
                     LWERGLLCRLDDRADPIIQIAPPLIAEGALFEEIAGILREGLEITAKQAGTGSRPDHG
                     SSTRAPEESSISPI"
BASE COUNT          184 a          394 c          360 g          218 t
ORIGIN      
        1 ctacctcctg ccgtacttcc tgctgatgat gatcgggctg agtccgatcg aagcgggcat
       61 cgccctgatc ccggcgaccg tgccgatcat gttggccgga cccctcgccg gccggctgtt
      121 cgaccgggtc ggaggccgca tcccgctggt catcggcttc ctggtccttg gcgcttcagg
      181 gattgccttc gccctggccg tcagcgagcg cgagtacgtg tatctgctcc ccggtctgat
      241 cctgcagggc atcgggctcg gcatggtcct cacggtgaac gacccgaccg gcctcgattc
      301 ggtcccgccg gaagaccagg ggcaggccgc cggggtgatc aacaccagcg agcagctcgg
      361 gggtgcggtc ggaatcgcat tgttctcagc ggtcctgatc aacacttcga ttcaccgcac
      421 caatgagtca ctggccgcga agggcatcca tcccaccgcc cgtcagacgg cggagtggaa
      481 ggagttcatc cagacgatca actccaaggg tctcggacac atcgacatct caaagcagtc
      541 cgagacgatc aggttcgccc tcaacgacgt gttgagcgcc catgtcgccg gttacgaggc
      601 tgtcttctac tcgaccgccg gcatcgccct gctgggtgcg ctcgcctgtt tcttcctggt
      661 ccgcaagacc gaccgggtgg aacagggcag ggttttcgtc cgccgttctc gctgggtcag
      721 ctcgaacacc ggccggaccc cggcggtcac aaagcatccg ccccggccca agtagccggc
      781 tctccgtacg aggtcgcgct cgatcagatc gggctgatcg agctttcctc gggcgcccgg
      841 gtcgaggaac cgtgatcggg ccgcgaaccg gtgcctgcct gcttcgcggt gatctcgagg
      901 ccttcgcgga ggatgccggc gatctcttcg aaaagtgccc cttcggcgat cagcggcggt
      961 gcgatctgga tgatcgggtc cgcccggtcg tccagccggc acagcaggcc gcgctcccag
     1021 agcgcctcgg aaaggacgtt tttgaaaagt tcctcggccg cctcaccctt gaacggttcc
     1081 ttcgtctcct ggtcacgaac cacctcgatc gcccagaagt gaccggtccc gcggacctgg
     1141 ccgacgatcg gaatgt
//
LOCUS       DAOQFI010000751         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_100083_length_1153_cov_3.328780, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000751 DAOQFI010000000
VERSION     DAOQFI010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100083_length_1153_cov_3.328780"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLE93_11155"
     CDS             <1..312
                     /locus_tag="PLE93_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HNH87652.1"
                     /translation="WSARLSPFKRAADLEPEEAERLLASIHDRFQAAIDFYESGLTLP
                     LPDKLPKPLEVHGREGEACSRCGELIHAVHYSDYTLCYCPREQTGGEPLKDRRLSRLL
                     K"
     gene            complement(335..595)
                     /locus_tag="PLE93_11160"
     CDS             complement(335..595)
                     /locus_tag="PLE93_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87653.1"
                     /translation="MTGPWRDLEQRLEFENEAERPLRESGEGESEGFEEAEAELIHQS
                     EDPDLGRDPELDAIVEAEDAGSVYSEADEILSSEEPDSDHDG"
     gene            complement(652..>1153)
                     /locus_tag="PLE93_11165"
     CDS             complement(652..>1153)
                     /locus_tag="PLE93_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87654.1"
                     /translation="GPSRYQRKMSDRELGIYLNDHLTGATGGVALARRAAANATDQER
                     KAMWKSIAHEVEEDRVTLTHVRDLIAVKPNRAKYALAWTAEKLGRLKLNGRLTGNSEL
                     GQMLELEALVIGVTGKLSLWKALARLEDPRLEGIDFDRLVERAESQRRRLEQFRINLV
                     PAAIGR"
BASE COUNT          177 a          401 c          356 g          219 t
ORIGIN      
        1 tggagcgccc gcctctcccc gttcaaacgg gcggccgacc ttgaacccga ggaagcggaa
       61 cggttgcttg cgtcgatcca cgaccgcttc caggcggcga tcgatttcta cgaatccggg
      121 ctcaccctgc cgctaccgga caaactgccg aagccgctcg aggttcacgg gcgtgagggt
      181 gaagcctgct cgcgctgcgg cgagctgatc cacgccgtcc attacagcga ctacacgctc
      241 tgctactgcc cgcgggagca gaccggcggc gagccgctca aggaccgtcg cctctcccgg
      301 ctcctgaaat aaacggggcg ggtcgagccg gcttctagcc gtcgtggtcg gagtccggtt
      361 cctcggaact gaggatctcg tcggcttcgg aatagaccga gcccgcgtcc tccgcttcga
      421 caatcgcgtc gagttcgggg tcgcgaccga ggtccggatc ctcggactgg tggatcagct
      481 ccgcttcggc ttcctcgaag ccttcggact caccttcgcc ggattcgcgc aaaggccgct
      541 cggcctcgtt ctcgaattcc agcctttgct caagatcacg ccagggaccg gtcatggcga
      601 caagatactg gccgggcaat actgctggac cgtccctccg cccgtcgatc gctacctgcc
      661 gatcgcggcc ggaaccaggt tgatccggaa ttgctcgagc cggcgccgct ggctttctgc
      721 ccgctcgacc aggcgatcga agtcgattcc ttccagacgg ggatcctcca gcctggcgag
      781 ggccttccag agcgagagct tcccggtcac gccgatcacc agcgcctcaa gctccagcat
      841 ctggccgagt tcggagtttc cggtcaggcg gccattcaac ttcagccggc cgagtttttc
      901 ggccgtccag gcgagggcgt acttggcgcg gttcggtttc accgcgatca ggtcacggac
      961 atgggtcagg gtcacgcggt cttcctccac ctcgtgcgcg atcgacttcc acatcgcctt
     1021 gcgctcctgg tcggtggcgt tggcggccgc tcgccgcgcc agagccacac ccccggtggc
     1081 gccggtcagg tggtcgttca ggtagatgcc cagttcgcga tccgacatct ttctctggta
     1141 ccgcgacggc ccg
//
LOCUS       DAOQFI010000752         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_100103_length_1153_cov_2.878871, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000752 DAOQFI010000000
VERSION     DAOQFI010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100103_length_1153_cov_2.878871"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1153
                     /locus_tag="PLE93_11170"
     CDS             <1..>1153
                     /locus_tag="PLE93_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87655.1"
                     /translation="GALIALAVIACAGFLVRAWVVVNPLADPGDDALAYRALAEALYV
                     DGTFGYGKDSSSGPEFKSPSDWSPGAPLIYAGSYYLTGGVRDGVARGVEALFGTAAIL
                     LAFLLTARLLGPPRQRNWRHALPPLVAAGLVAFYPPFIHSTGALMSEPPAFFMLPASV
                     LAFLWADRRMGEATGSGASGGKGSLWSRVWPWLLPGLGFGLTALIRPEYLSVCAAFTI
                     FLLIRAWMKSGAGPALAASALFVLAVLVPIVPWTVHNYHELDRVVPLSTGSGKALFTG
                     TYYPGDGEYQQVKAELYFQQTGNRLDPGSAELEAVDPVPLFDRVANEYKAANNLPNLD
                     RDDALGRLGKENLKEYLGDHPVGYVGMTFRKVGRMWSSGVGDAMSSPIGR"
BASE COUNT          149 a          421 c          371 g          212 t
ORIGIN      
        1 cggggcgctg atcgcccttg ccgtgatcgc atgcgccggg ttcctcgtcc gggcctgggt
       61 cgtggtcaac ccgctcgcgg accccggaga tgacgcgctc gcctaccggg cgctggccga
      121 agccctttac gttgacggga ccttcggcta cgggaaggac tcctccagcg gacccgagtt
      181 caaatcaccc agcgactggt cgccgggcgc cccgctcatc tacgccggct cctactacct
      241 gaccggcggc gtcagggacg gagttgcgcg cggcgtcgag gcccttttcg gcaccgccgc
      301 gatcctgctg gccttcctcc tgaccgcccg cctgctcggt ccgccgcgcc agcgcaactg
      361 gcggcatgcc cttccgcctt tggtcgccgc cggactggtc gccttctatc ctccgttcat
      421 ccactcgacc ggggcgctga tgagcgagcc gccggccttc ttcatgctgc cggcctccgt
      481 cctcgccttt ctctgggccg accgcaggat gggcgaagcc accggtagcg gcgcgagcgg
      541 cggaaagggt tcgctgtgga gtcgcgtctg gccctggctt ttgcccggcc tcggtttcgg
      601 cctgaccgcg ctgattcgcc ccgagtacct ctcggtctgc gccgccttca cgatcttcct
      661 gctgatccgg gcctggatga agtcgggggc cggaccggcg cttgcggcct cggcgctctt
      721 cgtgctcgcc gtgctggttc cgatcgttcc ctggacggtc cacaactacc acgagctcga
      781 ccgcgtcgtc ccgctttcca ccggcagcgg caaggccttg ttcaccggca cctactaccc
      841 cggtgacggc gagtaccagc aggtcaaggc cgagctctac tttcagcaga ccggcaaccg
      901 gcttgacccg ggttcggccg agctggaagc ggtcgatccg gttcccctct tcgaccgggt
      961 cgccaacgag tacaaggccg cgaacaacct gccgaacctc gaccgggacg acgccctggg
     1021 ccggctcggc aaggagaacc tgaaggagta tctcggcgat cacccggtcg gctatgtcgg
     1081 catgaccttc cgcaaggtcg gccggatgtg gagttccggg gttggcgacg cgatgtcctc
     1141 gccgatcggc cgg
//
LOCUS       DAOQFI010000753         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_100191_length_1153_cov_1.975410, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000753 DAOQFI010000000
VERSION     DAOQFI010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100191_length_1153_cov_1.975410"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLE93_11175"
     CDS             complement(<1..891)
                     /locus_tag="PLE93_11175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH87656.1"
                     /translation="MRCLVAAFGDPGHVFPAVSLALALKGRGHDVILETWPQWQGVVE
                     GVGLGFVSADQYQVFPPPASGSPGAGQAAVALMRLLEEFRPDVVVNDILTVAPALAAE
                     AHGCAWGTLIPHIYPVQDHGMPLFSVGGLPPRTGIGRGFWSFTERALKFGLERGRDDL
                     NDQRALVGLPPTDRFHGGTSPDLAMVATYPQLEYPRDWPGSVHVTGPMPFETPYEDIA
                     LPPGDEPLILVAPSTSQDPDNRLVRSALAALGDQPVRVVATTNRVVPSRPIEVPANSV
                     LVDWLSYSQLMPESSLVICHG"
     gene            complement(915..>1153)
                     /locus_tag="PLE93_11180"
     CDS             complement(915..>1153)
                     /locus_tag="PLE93_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87657.1"
                     /translation="KSTQKELMGTGVAVTAICPGFVATDMTEFAQQAVPAEEMMQPED
                     IAEAVSFLLKVSPACAVPELVMARPFAGPETQGM"
BASE COUNT          215 a          367 c          393 g          178 t
ORIGIN      
        1 gccgtgacag atcaccagcg aggactcggg catcagctgt gagtagctca gccagtcaac
       61 cagcaccgag ttcgcgggga cttcgatcgg ccggctcgga acgacccggt ttgtggtcgc
      121 caccacccga accggttgat cgccgagggc agcgagagct gatctgacga gacggttgtc
      181 gggatcctgc gaggtgctgg gagcgaccag gatcagcggt tcatcgccgg gtggcaaagc
      241 aatgtcttcg tacggggttt cgaagggcat cggcccggtg acgtggacag aaccgggcca
      301 gtcgcgaggg tactcgagtt gcgggtaggt agcgaccatt gccaggtcgg gactggtccc
      361 cccgtggaat cggtcggtcg ggggaaggcc gaccaacgcg cgctggtcgt tcaggtcatc
      421 ccgtccccgt tccagcccga acttcagggc tcgttcggtg aagctccaga agccgcggcc
      481 gatcccggtt cgtggcggca ggccaccgac cgagaagagg ggcatcccgt gatcctggac
      541 cgggtagata tgcgggatca gggttcccca ggcgcagccg tgagcttcgg cggccagggc
      601 gggagccacg gtgagaatgt cgtttaccac cacatcaggc cggaactcct caagcagtcg
      661 catcagagcc accgctgcct ggccggctcc gggggaaccg gaagccggag gcgggaacac
      721 ctggtactga tcggcgctga caaaacccag gccgacccct tcgaccacgc cctgccattg
      781 gggccatgtc tcgaggatca catcatggcc ccgtcccttg agcgcaaggg cgaggctgac
      841 cgcgggaaag acatggccgg gatcgccgaa agcggcgacc aggcaacgca aacttccgga
      901 acctgcggcc aggtctacat cccctgggtt tcgggtccgg cgaacggacg ggccattacc
      961 agctccggca ccgcgcaggc gggggaaacc ttgagcagga agctgaccgc ctcggcgatg
     1021 tcctccggct gcatcatctc ctcggccggc acagcctgct gggcgaactc ggtcatgtcg
     1081 gtggcgacga agcccggaca aatagcggtc accgcaaccc cggtgcccat cagctccttc
     1141 tgggtcgact tcg
//
LOCUS       DAOQFI010000754         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_100435_length_1151_cov_3.134124, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000754 DAOQFI010000000
VERSION     DAOQFI010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100435_length_1151_cov_3.134124"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLE93_11185"
     CDS             <1..265
                     /locus_tag="PLE93_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87658.1"
                     /translation="VLEKDGKPAPTLAVEGLRVDGRVGRKYGPDAGLVAALRRADDPP
                     VWLVTGGTDQAVRMAAAAPNPGDLARRYAAVVNDGQVSSLPLP"
     gene            274..>1151
                     /locus_tag="PLE93_11190"
     CDS             274..>1151
                     /locus_tag="PLE93_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNH87659.1"
                     /translation="MKKLSPIAFVPRRETVQSASLAPVALYLGAYLLVCFFSSDPLVL
                     VFATAGAALAGYGCGAGRAVNFSLKLGAFLALTMVIVNALVTSRGATVLARLGDWPLL
                     GQVEVTAEAIAAGMLIGLRVIGTMVVIGVWSACVDPDRILQAVHGIARRSALTATLIS
                     RLVPLAATDYARIGEAAELRGPAASPVSRGAMVHRLLAGSLDRAVDVAATLELRGYGL
                     SAESTRFRPAPSRFDRRFWIGGLLLTLLAFFELLAGSGRFVTYPGISYPVGAEGPLAA
                     LTFLLCGFVTWRRGGRG"
BASE COUNT          138 a          408 c          390 g          215 t
ORIGIN      
        1 cgttctcgag aaagatggca aaccggctcc gacccttgca gtcgaaggac taagggtcga
       61 tggccgcgtg ggaaggaagt acggccccga tgccggcctc gtggccgcat tgcgccgagc
      121 cgacgatccg cccgtctggc tggtcaccgg cggaacggac caggcggttc ggatggcggc
      181 cgcggcgccc aacccgggcg accttgcccg caggtacgcg gcggttgtaa acgacggaca
      241 ggtctcgtcc ctgcccttac cctgaggccg gccatgaaga agctctcccc gatcgccttc
      301 gtgccccggc gggaaaccgt tcagtcggcc tcgctcgcgc cggttgcgct ctacctcggc
      361 gcctacctgc tggtctgctt cttttcttcc gaccccctgg tactggtttt cgccacggcc
      421 ggcgcggcgc tcgccggtta cggatgcgga gccggccggg cggtgaactt ctcgctgaag
      481 ctcggtgcct tcctcgctct gaccatggtc atcgtcaacg cgctggtcac cagccgcgga
      541 gccaccgttc tcgcgcggct cggtgactgg cccctgctgg ggcaggtcga agtaaccgcg
      601 gaggcgatcg ctgcgggcat gctgatcggt cttagggtga tcggcacgat ggtcgtgatc
      661 ggcgtctggt cggcctgcgt cgacccggac cggatcctgc aggcggtcca cggcatcgcc
      721 cgccgctccg ctctcaccgc aacccttatc tcgcgcctgg ttcccctcgc ggccaccgat
      781 tacgcccgga tcggggaggc ggcggagctt cgcggcccgg cggccagccc ggtcagccgg
      841 ggggcgatgg tgcaccggct gctggccggt tcactcgacc gggcagttga cgtggcagcc
      901 acgctcgagc ttcgcggtta cgggctatcg gctgagtcca cccggttcag gccggcccct
      961 tcgcgtttcg atcgccgctt ctggatcggc ggtttgctcc tgaccctgct cgccttcttc
     1021 gagctcctgg ccgggtccgg tcgtttcgtc acctaccccg ggatcagcta cccggtcggg
     1081 gccgaagggc cgctcgccgc cctcaccttc ctgctttgcg gattcgtgac ctggcggcgg
     1141 ggtggcaggg g
//
LOCUS       DAOQFI010000755         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_100739_length_1150_cov_1.557078, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000755 DAOQFI010000000
VERSION     DAOQFI010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100739_length_1150_cov_1.557078"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..1119)
                     /locus_tag="PLE93_11195"
     CDS             complement(295..1119)
                     /locus_tag="PLE93_11195"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNH87660.1"
                     /translation="MTSDGRSIARPAGDAGGASGRHELTALARGPWQEDAAHGGAPAG
                     LLARAAESLGADENLRLASISLAFNGPVFLGEIEVRAEVVKPGRRQRVVAVAIDAGGR
                     TLIEGRAVLIRRGEVDLPDGVRVVEDSWPGPDQGRPIEQELWAHGESEAFHRTSNTVV
                     AIEGGPDRIGTTGMVWIRLDYPLVPGEELTGAQRAVAAADFGNGVAHPVGWGDFLFIN
                     CDLNVSLLREPTGEWIGLESRTEVDANGSGLPTPRLHDANGHFGNASQILFVDRAA"
BASE COUNT          185 a          390 c          376 g          199 t
ORIGIN      
        1 atcacttcct tgcgtgaaat cgaactcgtg ttggtgacca gcctgatgcc tccggccagt
       61 tcgcgcagct gattcagcgt ctcagtggcg ccatcgatcg cctcgccctg gatatagagc
      121 acaccctcga catcgagcag gatcgcatcg ggcttttcgg gcaaaccggg catgtaccaa
      181 atgtattccg tggccagagg ccgaaacctg tcccgcggga caaaccggct gggctgaaat
      241 ccactgagat tgaatccgtc ggcaaatagc gccgacagaa tcaaggtcag gccatcaggc
      301 ggcccggtcc acgaagagga tctgactggc gtttccgaag tggccgtttg cgtcgtggag
      361 gcggggggtg gggaggccgc tgccgtttgc gtctacctcg gtccttgact cgaggccgat
      421 ccactctccg gtcggttccc tgaggagtga gacgtttaga tcacagttga tgaacaggaa
      481 gtcgccccag ccgaccgggt gggcgacgcc gttgccgaag tcggcagcgg cgaccgctcg
      541 ctgggctccg gtcaattcct cgccgggcac cagcgggtag tcgagccgga tccagaccat
      601 gcccgtggtg ccgatccggt ccgggccgcc ctcgatcgcg acgaccgtat tgctggtccg
      661 gtggaatgcc tcgctttccc cgtgcgccca cagttcctgc tcgatcggcc gcccctggtc
      721 cggacccggc caggaatctt caaccaccct gacgccgtcc ggcaggtcca cctcgccacg
      781 gcggatcagt accgcccggc cttcgatcag ggtgcgtccg ccagcgtcga tcgccacggc
      841 gacgacccgc tggcggcggc ccggcttgac gacttccgcc ctgacctcga tctcgccgag
      901 gaagaccgga ccgttgaagg caagagagat cgaagccagc ctcaggttct cgtcagcccc
      961 gaggctctcg gcggcgcggg ccagcagacc ggccggggca ccgccgtggg cggcgtcttc
     1021 ctgccacggc ccgcgggcca gggcggtcag ttcgtggcgt ccggaggcgc cgccggcatc
     1081 acctgccggc ctggcgatcg accgtccgtc gctggtcatt cagcgttgct cgcggcaagg
     1141 aggattccgg
//
LOCUS       DAOQFI010000756         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_100793_length_1149_cov_3.124314, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000756 DAOQFI010000000
VERSION     DAOQFI010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100793_length_1149_cov_3.124314"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..928
                     /locus_tag="PLE93_11200"
     CDS             194..928
                     /locus_tag="PLE93_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HNH87661.1"
                     /translation="MKLRLLSLYPDQMNIYADRGNILFLQHRCEWRGIEFEVTRCGPG
                     QRFDPANYDLLYMGGGQDRDQKAVARDLIETKQASMRAAKDGGVAVLAVCGGYQLLGE
                     SYELEGETIAGLGLVDLKTIREPGPRLIGPVSISVNLDGEEKVLAGFENHGGRTRLGQ
                     ASPLGKVIHGHGNNGEDGFEGVREDNLIGTYLHGPLLPKNAWLADHLIATGLEHHHGE
                     RPELEPLEDSLETAAHEAALKAARKG"
     gene            complement(925..>1149)
                     /locus_tag="PLE93_11205"
     CDS             complement(925..>1149)
                     /locus_tag="PLE93_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNH87662.1"
                     /translation="GISLVNAPGLVDAGYRGEVKVLLLNNDPAEVFRINPGDRIAQMV
                     VIPLAAVEPVATDSLSETERGEGGFGSSGR"
BASE COUNT          220 a          388 c          371 g          170 t
ORIGIN      
        1 ggctcgttcg aggaatcccg agaccgcatc accctggacc ggatcgaccg gaagtcgctg
       61 gccgatgaac tcgccgaagc cggcctttcg gcggttcgtg cgatcgacgt gccggagacc
      121 gaccgacaca tcgcgaccgt gatcgtggtc gcccgtcaca gtccccgagg gggctgaacc
      181 ctagggtttg cgtatgaagc ttcgcctgct cagcctctac ccggaccaga tgaacatcta
      241 cgccgaccgg ggcaacatcc tctttctgca gcaccgctgc gagtggcgcg ggatcgagtt
      301 cgaagttacc cgctgcgggc cgggccagcg cttcgacccg gccaattacg acctgctcta
      361 catgggtgga ggccaggacc gggaccagaa ggcggtcgcc agggacctga tcgaaaccaa
      421 acaggcttcg atgcgcgcgg ccaaggatgg cggcgtggcc gtgctcgccg tctgtggcgg
      481 ctatcagctc ctgggcgagt cctatgagct ggaaggcgaa acgatcgccg gcctcggcct
      541 ggttgacctg aaaaccatcc gcgaaccggg cccaaggctg atcggaccgg tttcgatcag
      601 cgtcaacctt gacggtgaag agaaagtcct ggccgggttc gagaaccacg gcggaaggac
      661 ccgcctcggc caggcgtcac cgctcgggaa ggtcatccac ggccacggga acaacggaga
      721 ggacggtttc gaaggggtcc gcgaggacaa cctgatcggc acctacctgc acggccccct
      781 gctcccgaag aacgcctggc tggccgatca cctgatcgcg accgggctcg agcatcacca
      841 cggcgagcgg cccgaactcg agccgctcga agattcactc gaaacagccg cccacgaagc
      901 ggcgctcaag gcagcccgca agggctagcg gccgctcgag ccgaagccgc cctcgccccg
      961 ttcggtctcc gacagcgagt cggtcgcgac cggttccacc gcggcaagcg ggatcacgac
     1021 catctgagcg atgcggtcgc ctgggttgat ccggaatacc tcggccggat cattgttcag
     1081 cagcagcacc ttcacctcac cgcggtagcc ggcgtcgacc aggcccggcg cgttgaccag
     1141 ggaaatgcc
//
LOCUS       DAOQFI010000757         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_100981_length_1148_cov_3.352242, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000757 DAOQFI010000000
VERSION     DAOQFI010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100981_length_1148_cov_3.352242"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1148
                     /locus_tag="PLE93_11210"
     CDS             <1..>1148
                     /locus_tag="PLE93_11210"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HNH87663.1"
                     /translation="QITGMTSDTNGGCEPLSAGADVIRAQEGCNEDVVPVSVIFDPLS
                     NPGGVRCTLWDNMINVYGADPATGYARRTLDNVGVQYGLGALKGGSINMKRFLDLNEQ
                     IGGYDNNGNFQAGRTVADQDALKTSYQTGRIQQGAGGWTQVPIIDVRNYQDDVAAGNV
                     HQYVNTYRTRARLDRFNGNHDNQVMFRAIGGPSVKAMNDTAIDTLSTWLDSIVADDSS
                     KSLAQKVVDNKPADAVDACWIAGNRVDGVAQAGDTNTCETTYPPHSLPVNVAGKPLDS
                     ITAKCTLKPVNPADYGSPTPEQITRLNQIFPDGVCDWSQAGPEETTVQGTDLSFGPSH
                     EAGDANRHLGLKLNRYKLNYSRKGGSVTATATLGNCPAVTWQKVRFER"
BASE COUNT          238 a          406 c          344 g          160 t
ORIGIN      
        1 gacagatcac cggcatgacc tcggacacca acggcggctg tgaaccgctt tcggccggcg
       61 ccgacgtcat tcgcgcccag gaaggctgca acgaggatgt ggtccccgtc tccgtgatct
      121 tcgatcccct gtccaatccg ggcggcgtcc gctgcacgct ctgggacaac atgatcaacg
      181 tctacggggc tgacccggcc accggctacg cccgccgcac gctcgacaac gtcggggtgc
      241 agtacggcct cggggcgctt aagggcggat cgatcaacat gaagcgattc ctcgacctga
      301 acgagcagat cggcggatac gacaacaacg gcaacttcca ggccggtcga accgtggctg
      361 accaggatgc gctgaagacc agctaccaga ccggccgcat ccagcaggga gccgggggat
      421 ggacccaggt gccgatcatc gacgtccgca actaccagga cgacgtagcg gccggcaacg
      481 tccatcaata cgtcaacacc taccgcaccc gggcccggct cgaccgcttc aacggaaatc
      541 acgacaacca ggtcatgttc cgggcgatcg gcggccccag cgtgaaggcg atgaatgaca
      601 cggcgatcga cacgctctcc acctggctcg actcgatcgt cgccgatgat tcttcgaagt
      661 cactggccca gaaggtggtc gacaacaagc ccgccgacgc ggtcgacgcc tgctggatcg
      721 ccggcaaccg ggtcgacggc gtcgcccagg ccggtgacac gaacacctgc gagacgacct
      781 acccgccgca ttcgctgccg gtgaacgtgg ccggcaagcc gctcgattcg attaccgcca
      841 agtgcaccct caaaccggtc aatccggccg attacggctc acccaccccg gagcagataa
      901 cccgcctcaa ccagatcttc ccggacgggg tctgcgactg gtcgcaggcg ggaccggaag
      961 agaccaccgt ccagggcacc gatctctcct tcgggccttc ccatgaggcc ggcgatgcga
     1021 accgccacct gggcctgaag ctgaaccggt acaagctcaa ctacagccgc aagggcggca
     1081 gcgtcacggc gaccgcgacc ctgggcaact gccccgcggt cacctggcag aaagtccgct
     1141 tcgagcga
//
LOCUS       DAOQFI010000758         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_101019_length_1148_cov_2.605672, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000758 DAOQFI010000000
VERSION     DAOQFI010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101019_length_1148_cov_2.605672"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLE93_11215"
     CDS             <1..220
                     /locus_tag="PLE93_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87664.1"
                     /translation="RFERQLRQGRKWVWKNIGSKMVTGKDCQASLTVRNIKGKTNLRA
                     RALAITGFKAANSKLRTVTVKKPKRHRR"
     gene            271..344
                     /locus_tag="PLE93_11220"
     tRNA            271..344
                     /locus_tag="PLE93_11220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:305..307,aa:Gly,seq:gcc)
     gene            377..447
                     /locus_tag="PLE93_11225"
     tRNA            377..447
                     /locus_tag="PLE93_11225"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:409..411,aa:Cys,seq:gca)
     gene            complement(534..980)
                     /locus_tag="PLE93_11230"
     CDS             complement(534..980)
                     /locus_tag="PLE93_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HNH87665.1"
                     /translation="MAAATLVLALGISACGSSSDDSGESSDTGTTTSTTQAPANAQSV
                     KISLTEMAISASPDSVKAGDVAFDVTNDGNLEHELVVVKTNKKAGDLPTDASGKADET
                     GSVGDTEVQAGESSELDLKLAAGHYALICNLPGHYKAGMYTDFTVK"
BASE COUNT          201 a          356 c          365 g          226 t
ORIGIN      
        1 ccgcttcgag cgacagctca ggcagggccg caagtgggtc tggaagaaca tcggttcgaa
       61 gatggtcacc ggaaaggatt gccaggcctc gctcacggtc aggaacatca agggcaagac
      121 gaacctgcgg gcccgggcgc tggcgatcac cggcttcaag gccgcgaact cgaagctccg
      181 cacggtcacg gtgaagaagc cgaagcgtca ccgtcgctga ccggcggggc gtcgcctgag
      241 acccttcccc ggatatactc cgccgacttc gcggctgtag ctcagttggc tagagcgtct
      301 gcttgccatg cagaaggtcg agggttcgaa tcccttcagc cgcttccggg acaccccttg
      361 cggggcattc ccattcggcg ccttggcgga gtggctacgc agcggcctgc aaagccgttt
      421 acaccggttc gactccggta ggcgcctttc tgccggcgtc cctgaacggt gcggattgaa
      481 aagcgaggcg gacggtcggc tgcccgcccc tctttccgtc cggaattccc ggtctacttg
      541 accgtgaagt ccgtgtacat accagccttg tagtggccgg gcaggttgca gatgagcgcg
      601 tagtggccgg cagcgagctt caggtcgagc tctgaactct cgcccgcctg aacctcggtg
      661 tctccaaccg aaccggtctc gtccgctttc ccggaagcgt cggtaggcag atcgcccgcc
      721 ttcttgttcg tcttgaccac taccagctcg tgctcgaggt tcccgtcatt ggtcacgtcg
      781 aaggccacgt ccccggcctt cacggagtcc ggcgaagcgc tgatcgccat ctcggtcaaa
      841 ctgatcttga cggactgggc gttggccgga gcctgggtag tcgaggttgt ggtgccggtg
      901 tcgctgctct ctccggaatc atcgctcgag gatccacaag cggagattcc cagggcaaga
      961 acaagcgttg ccgcagccac agatacacgc aatgcgtttg gcttcaaagg gaggcctttc
     1021 tgactaggtt caccaggacg gaagacctgg cgacgcttga gtttacgcct gggagtcgag
     1081 ggcctggttg accagcgcgt cggcttcggc gttctgttca cggcggacgt gactgatcga
     1141 ccacgagt
//
LOCUS       DAOQFI010000759         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_101150_length_1147_cov_3.971612, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000759 DAOQFI010000000
VERSION     DAOQFI010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101150_length_1147_cov_3.971612"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PLE93_11235"
     CDS             complement(<1..576)
                     /locus_tag="PLE93_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH87666.1"
                     /translation="MRVVIADDHGIVRSGIRLLLERQGDLEVIAEAADGIEARDTVVR
                     ERPDLAILDVKMPGLTGLQATREIKAQTPETNVLILSMHDDERYLFEALKAGASGYVL
                     KSQADTDLLDAIRSVERGEPFLSPGAQQTLIRDLLERGEPAGSDLTPREEEIVKLVAE
                     ANTTREIAGMLHLSEKTVENHRANAMKKLGMR"
     gene            complement(573..>1147)
                     /locus_tag="PLE93_11240"
     CDS             complement(573..>1147)
                     /locus_tag="PLE93_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNH87667.1"
                     /translation="LHDQVNQSLTGVLLRLEATRSEAPEELAPEIEETSRLAHQAMDE
                     LLTVARQLRPTALDDLGLRAAIAGLVEQLDRPGLTATFTCDDDPGGLDPDIQLVIYRV
                     SQEAIGNAVRHSGASRVDAVLARNGDRFELKVTDDGSGFSFDQATSGLGLGGMRERAL
                     LVGGELDVESRTGHGTTIRLSVPVREGNPG"
BASE COUNT          163 a          380 c          402 g          202 t
ORIGIN      
        1 ccgcatgccg agcttcttca tcgcgttggc ccggtggttt tcaacggtct tctcggaaag
       61 gtggagcatc ccggcgattt ccctcgtcgt gttggcctcg gccacgagct tcacgatctc
      121 ttcctcccgc ggtgtgagat cggacccggc cggttcgccc cgttcgagca gatcgcgaat
      181 cagggtctgc tgggcgccgg gggagaggaa gggctcgccg cgctcgaccg accgaatcgc
      241 gtcaagcagg tcggtgtcgg cctgggactt gagcacgtaa ccggaggcgc cggccttcag
      301 cgcctcgaac aggtagcgct cgtcgtcgtg catcgagagg atcaggacat tggtctccgg
      361 ggtctgggcc ttgatctcgc gggtcgcctg cagtccggtc agaccgggca tcttcacgtc
      421 gaggatggcc aggtcgggcc tttcgcggac aaccgtgtcg cgggcctcga tcccgtcggc
      481 ggcttcggcg atcacctcga gatcgccctg gcgctcgagc agcagccgga ttccggaacg
      541 gacgatcccg tggtcgtcgg caatcacgac cctcatccgg ggtttccttc ccggaccggg
      601 accgacaacc ggatcgtggt cccgtggccg gtgcgcgatt cgacgtcgag ttccccgccg
      661 accaggagcg cccgctcacg catgccgccg aggccgagcc ccgaggtggc ctggtcgaag
      721 ctgaaaccgc tgccgtcatc ggtgaccttc agctcgaagc ggtccccgtt gcgggcaagg
      781 acggcatcga cccggctggc cccgctgtgg cggaccgcgt tgccgatcgc ttcctgggag
      841 acccggtaga tgaccagctg gatgtccggg tcaagcccgc cggggtcgtc atcgcaggtg
      901 aaggtggcgg taaggccggg gcggtcgagc tgttcaacca ggccggcgat cgcagcgcga
      961 aggccgaggt catccagcgc ggttggccgc agctggcggg ccacggtcag aagctcatcc
     1021 atcgcctgat gcgcgagccg gctggtctcc tcgatttccg gcgccagctc ctcgggggcc
     1081 tcggagcggg tcgcctcgag cctcagcagc acgccggtca gcgactggtt gacctggtcg
     1141 tggaggt
//
LOCUS       DAOQFI010000760         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_101178_length_1147_cov_3.020147, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000760 DAOQFI010000000
VERSION     DAOQFI010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101178_length_1147_cov_3.020147"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PLE93_11245"
     CDS             complement(<1..565)
                     /locus_tag="PLE93_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87668.1"
                     /translation="MPLSEDSRALLQLLLGRGKNYADISGLLGIDEAEVRSRAGTALA
                     ELDDSNRPPDPELTDYLLGQADPIGRADVGNRISSDPALAERAESITDQLRLLVPGAE
                     LPRPGSAEGGKPKPATKPPAPVAAAAPETGPAADPAGAQGRSGGFSSITSHQRRLIAV
                     LLGAALLAAVVILLVTGVFGGGSGDDEK"
     gene            672..1100
                     /locus_tag="PLE93_11250"
     CDS             672..1100
                     /locus_tag="PLE93_11250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNH87669.1"
                     /translation="MPDVTLPESPFDNLLGTEWVSSDPDQAIVRLTMRDDHRQPMAIM
                     HGGVMASLVESVCSMATARAVLPEGRIAMGQNISVNLLRPISSGEVEVVATAVHRGRM
                     TWVWRAEVKDDEGRVCAISQMTMAVREAPEGADLSQFVKK"
BASE COUNT          182 a          379 c          394 g          192 t
ORIGIN      
        1 ttttctcgtc atcgccggag ccgccgccga agaccccggt caccagaagg attacgaccg
       61 cggccagcag ggcggcgcca agcagcaccg cgatcaggcg tcgctggtga ctggtgatcg
      121 aggagaaacc gccggagcga ccctgagcgc cggccgggtc tgcggcgggc ccggtctctg
      181 gcgccgcggc agcgaccggt gcggggggct ttgttgccgg cttcggcttg ccaccttcgg
      241 ccgatcccgg acgcggcagt tcggccccgg ggacgagcaa cctgagctga tcggtgatcg
      301 actccgcccg ttcggcaagg gcggggtccg atgaaatcct gttgccgacg tcggcccggc
      361 cgatcggatc ggcctgaccg agcagatagt cggtgagttc cgggtccggg ggccggttcg
      421 agtcatccag ttcggccagc gcggtgccgg cgcggctgcg cacctcggcc tcgtcgatcc
      481 ccagcaggcc cgaaatgtcg gcgtaattct tccctcggcc aagcagcagc tgcagcagcg
      541 cccggctgtc ctcactcaac ggcatgacgg tgaaccctac gggttcggcg cggttggctg
      601 tttcctgagt ggaccctgtg aagaagccgg gttcaccggg cctccggttc ggacccgtat
      661 gatcccggcc gatgcccgac gtaacgcttc ccgaatcccc gttcgacaac ctgctcggca
      721 ccgagtgggt ttcctccgac cctgatcagg ccattgtccg cctgaccatg cgtgacgacc
      781 atcggcagcc gatggcgatc atgcacggcg gggtcatggc cagcctggtc gagagcgtct
      841 gctcgatggc cacggcccgg gccgtgctgc ccgagggaag gatcgccatg gggcagaaca
      901 tctcggtcaa cctgctgcga ccgatctcga gcggtgaggt cgaggtagtc gccaccgcgg
      961 ttcaccgggg ccgcatgacc tgggtctggc gggccgaagt caaggacgac gaggggcgtg
     1021 tctgcgcgat ctcacagatg accatggccg tccgcgaagc ccccgaaggc gcagaccttt
     1081 cgcagtttgt gaagaagtaa ctcagtcaga gccctcccat ccattgaatg tctcagtggg
     1141 cgggcgg
//
LOCUS       DAOQFI010000761         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_101650_length_1144_cov_5.072544, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000761 DAOQFI010000000
VERSION     DAOQFI010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101650_length_1144_cov_5.072544"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLE93_11255"
     CDS             <1..861
                     /locus_tag="PLE93_11255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH87670.1"
                     /translation="HLARRFNAPLVTTIHATEFGRHQGWVDKHPQSHIHGVEKWIVNR
                     SDRVIACSHFMREQIADIFGVEEGRISVIPNGIDPDDLPAEDPVELARLRAQFADPDE
                     NLVLLIGRLVYEKGFQLALEAMPAVIEKLPNTRFLVAGSGTHEAELHKQAKDLGLMEH
                     GTFLGWIGDDVLHSLYRIADLTVVPSIYEPFGLVALEAMASECPCIAADTGGLREVVP
                     IEGAGLRFRASDPEHLAEAAIQVLGDPELSKRMIEEGLAHIRRFDWDEIGEQTLDLYE
                     GLVASTDVRG"
     gene            872..>1144
                     /locus_tag="PLE93_11260"
     CDS             872..>1144
                     /locus_tag="PLE93_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87671.1"
                     /translation="MKRLWGSLIVLLAFALAFTATSQAAFSVSYTDRGSLWLSSLDGK
                     HKRKLAAKPNGKVKWIETAQSDNGRVIAVRRDPAKIANLNSYSLWGP"
BASE COUNT          198 a          371 c          365 g          210 t
ORIGIN      
        1 cacctcgccc ggcgcttcaa cgctccgctg gtcacgacga tccacgcgac cgagttcggc
       61 cgccaccagg gctgggtcga caagcatccc cagtcgcaca tccacggcgt ggaaaagtgg
      121 atcgtcaacc gttccgaccg ggtgatcgcc tgttcccatt tcatgcgtga acagatcgcc
      181 gacatcttcg gcgtcgagga aggccggatc tcggtgatcc cgaacggaat cgaccccgac
      241 gacctgccgg ccgaggaccc ggtcgagctt gcgcgcttgc gggcgcagtt cgcggatccg
      301 gatgagaacc tggtcctgct gatcggccgc ctcgtctacg agaagggttt ccagcttgcg
      361 ctggaggcga tgccggcggt gatcgagaag ctgccgaaca cccggttcct ggtcgccggg
      421 tccggaaccc acgaggctga acttcacaag caggccaagg acctcggcct gatggaacac
      481 ggcaccttcc tcggctggat cggggacgac gtgcttcatt cgctctaccg gatcgccgac
      541 ctgacggtcg ttccgtcgat ttacgagccc tttggtctgg tcgccctcga agcgatggct
      601 tcggaatgtc catgcatcgc cgccgacact ggcggcttgc gggaggtggt gccgatcgag
      661 ggcgccggcc ttcgctttcg tgcgtcggac ccggaacacc tggccgaggc cgcgattcag
      721 gttctcggtg accccgaact cagcaagcgg atgatcgaag agggtctcgc ccacatccgc
      781 cgttttgact gggatgagat cggcgagcag acgctcgatc tctacgaagg actggtcgct
      841 tcgaccgacg ttcgcggcta gtatcagggc catgaagagg ctatgggggt ccctgatcgt
      901 gttgctggcg ttcgctctgg cgttcaccgc gacttcacag gccgctttct cggtttccta
      961 caccgaccgg ggcagcctct ggctttcctc gctggacggg aagcacaagc gcaagctcgc
     1021 cgcgaagccg aatggcaagg tcaagtggat cgagaccgcc cagtcggaca acggcagggt
     1081 gatcgccgtc cgtcgcgacc cggccaagat cgcgaacctg aactcctact ccctgtgggg
     1141 gccg
//
LOCUS       DAOQFI010000762         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_101758_length_1144_cov_2.042241, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000762 DAOQFI010000000
VERSION     DAOQFI010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101758_length_1144_cov_2.042241"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PLE93_11265"
     CDS             <1..1073
                     /locus_tag="PLE93_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87672.1"
                     /translation="IPLAWLLARRLAPPEDPGVAGIVAAAAVAFYPCLIADAGMLLTE
                     ALAGTLVTGALLSMLRARDHLGEPGRPLWLRTTDWLLPGALLGMTAMVRPEYLPISLA
                     LILVLALISRGHGLRPVLFGSALMLLAVGLVITPWGTRNLDHSGRLVPLSTGGGQTLF
                     TGSYLASAGNPQEVMPDLLARNPDLARRIQVQNDASGEGPASITPERVLALLAAERMP
                     GVPTDVALARIGRENYLRALRQDPARLLGYFAAKSARIWWRGRKDLTGTTTGLVSHMT
                     LVAGALLGLLLLGFRRQPEFWLILALAVGVTLIGAVFVASPRRTLAFWPVIACLSGIG
                     FSLALRLARDAIAARWRPVPIA"
BASE COUNT          134 a          440 c          358 g          212 t
ORIGIN      
        1 cgattcccct ggcctggctt ctggccagaa ggctcgcccc tccggaggat cccggggtcg
       61 ccggaatcgt cgcggccgcg gcggtcgcct tctacccctg cctgatcgcc gacgccggca
      121 tgctgctgac cgaagcgctg gccggcaccc tggtcacggg tgccctgctg agcatgctcc
      181 gggcccgcga tcatctgggc gagccgggcc gtccgctctg gctcaggacc accgactggt
      241 tgctgccggg agccctgctc ggaatgaccg cgatggtcag gcccgagtac ctgccgattt
      301 cgctggccct gatccttgtc ctcgccctca tctcccgtgg ccacggactt cgaccggtcc
      361 tgttcgggtc ggccctgatg ctgctggcgg tcgggttggt gatcactccc tggggcaccc
      421 ggaacctcga ccattccggc cggctcgttc cgctctccac cgggggcggc cagacgcttt
      481 tcaccggcag ctacctggct tcggccggca atccgcagga ggtgatgcct gatctgctcg
      541 cccgcaatcc cgacctcgcc agaaggatcc aggtccagaa cgacgccagc ggggaaggtc
      601 ccgcctcgat cacaccggaa cgggtcctgg cgctgctggc cgccgagcgc atgcccggcg
      661 ttcccaccga cgtggccctc gctcgcatcg gccgggaaaa ctacctccgc gccctgcggc
      721 aggaccccgc ccgcctgctc gggtatttcg ctgccaaatc ggcccggatc tggtggcggg
      781 gccgcaagga cctgaccggg accacgactg gcctggtatc ccacatgacc ctggtcgccg
      841 gcgccctgct cggtctgctc ctgctcgggt tccgccgcca gcccgagttc tggctgatcc
      901 ttgccctggc cgttggcgtc accctgatcg gagcggtctt cgtcgcctcg ccccgccgca
      961 cccttgcctt ctggccggtg atcgcctgcc tttccgggat cggcttctca ctggctctcc
     1021 gccttgcgag agatgccatc gccgccagat ggcgcccggt tcccatagcc tgaccggccc
     1081 ggatgccgct tctcaaggat tacttcaccc gctacggccg cggggcgctg gtcgcccttg
     1141 ccgt
//
LOCUS       DAOQFI010000763         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_101952_length_1143_cov_2.153493, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000763 DAOQFI010000000
VERSION     DAOQFI010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101952_length_1143_cov_2.153493"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLE93_11270"
     CDS             complement(<1..1067)
                     /locus_tag="PLE93_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87673.1"
                     /translation="MPGSLKSVSRKLIDQFHSFPLAFIGVGKPGAWDRLVILRPKLVK
                     GRSQAITLSRLVVDPVAPTTHDLSVISGLQWDSTKSDTENHQAIDNALDVERVTRQFF
                     RELNSHYQAIVQGVEKLAKEDPSIYSGVDAAGGHERVALRIITQTLFCYFLQKKGLLE
                     KNPKWLSVQFDNWTKSGEEGSFYADVMESLFYGMLNTPVDERDSSLAGRQVPFLNGGL
                     FERRYGGISIELPNELFNTDDGLLGFLDGWNFTVSEERADETEVAVDPEMLGKVFENL
                     MADHDKKSQGAVYTPRPVVQFMCREALVPYLQRKADIPENQARILIDGDDPFVELAEA
                     TTVEHSAEVADAVEEAMTTIQV"
BASE COUNT          223 a          347 c          321 g          252 t
ORIGIN      
        1 acctggatgg tggtcatcgc ctcttcgacg gcgtctgcca cctcggctga gtgctcgacc
       61 gttgtcgcct ctgccagctc aacgaatgga tcgtcaccgt cgatcaggat ccgcgcctgg
      121 ttctccggga tatcggcttt gcgctggagg tacggcacca aggcttcccg acacatgaac
      181 tgaaccacag gcctgggcgt gtagacggca ccctgagatt tcttgtcatg gtcagccatc
      241 aggttctcga aaaccttccc gagcatttcc gggtcgacag caacctcggt ttcgtcagcg
      301 cgctcctccg acaccgtgaa gttccacccg tcaaggaaac cgagcagccc atcatcggtg
      361 ttgaacagct cattcgggag ctcgatggat attccgccgt agcggcgttc gaaaagacca
      421 ccgttcagga agggaacctg tcggccagca agactggaat cacgctcatc aaccggggtg
      481 ttgagcatcc cgtagaagag gctctccatg acgtcggcgt aaaagcttcc ctcttccccg
      541 ctcttggtcc agttgtcgaa ctgaaccgaa agccacttgg ggttcttctc gagcagcccc
      601 ttcttctgga ggaaatagca gaagagggtc tgagtgatga tccgcaacgc aacccgttca
      661 tgtccgccgg ccgcgtccac ccccgagtag atcgaaggat cctcctttgc cagcttctct
      721 accccttgaa cgattgcctg gtagtggctg ttgagttcac gaaagaactg gcgggtgacg
      781 cgttcaacgt caagcgcatt gtcgatcgcc tgatgattct cggtgtccga cttggtggaa
      841 tcccactgca gtccggatat gacgctcaga tcgtgggtgg ttggcgcgac cggatccacg
      901 accagtcgac ttagggtgat ggcctggctt ctccccttga ccagtttggg gcgaagaatc
      961 accagtcggt cccaggcacc gggctttccg acccctatga acgcgagcgg gaagctgtgg
     1021 aactggtcga tgagtttccg gcttaccgac ttcagcgatc ccggcaacga ttccacttcc
     1081 gcgacaacga gaccgtagct cttgtgtttg gcgttcgcgc cacggatcct gaatgcggtc
     1141 cct
//
LOCUS       DAOQFI010000764         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_102197_length_1141_cov_3.348066, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000764 DAOQFI010000000
VERSION     DAOQFI010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102197_length_1141_cov_3.348066"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1141)
                     /gene="carB"
                     /locus_tag="PLE93_11275"
     CDS             complement(<1..>1141)
                     /gene="carB"
                     /locus_tag="PLE93_11275"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934674.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNH87674.1"
                     /translation="DPMGVHTGDSITVAPAQSLTDPVYQSLRDIAIAVIREVGVETGG
                     SNVQFAVNPADGEIVVIEMNPRVSRSSALASKATGFPIAKMAAKLAVGYALEEIPNDI
                     TRATPASFEPTIDYVVTKIPRFAFEKFPGADEKLSTYMQSVGEVMSIGRTFRESFAKA
                     LASRELDSRPEVPESDNGLLNAIATPTPGRIDLVLAAFERGLTVEQVHEQAKIDPWFL
                     RELELLATDGDGTGGLERIYNSVDTCAAEFEAATPYYYSSHEPEGSPSEIRRGEKESV
                     VILGAGPNRIGQGIEFDYCCVHAAMTARECGRDAVMVNCNPETVSTDYDTSDRLYFEP
                     LTLEHVLAVCEKEQPEGVIVMFGGQTPLKLAEGLKEAGINLLGTPV"
BASE COUNT          177 a          376 c          384 g          204 t
ORIGIN      
        1 cgaccggggt gccgagcagg ttgatgccgg cttccttgag accctcggcc agcttcagcg
       61 gcgtctggcc gccgaacatc acgatcaccc cttccggctg ttccttctcg cagaccgcca
      121 gcacgtgttc gagcgtcagc ggctcgaagt agagccggtc cgaagtgtcg taatcggtcg
      181 agaccgtttc cggattgcag ttgaccatca ccgcgtcgcg tccgcactcg cgggcggtca
      241 tcgcggcgtg gacgcagcag tagtcgaact cgatcccctg gccgatccgg ttcgggccgg
      301 ccccgaggat caccacagat tccttctcac cccggcgaat ctcggagggt gacccttccg
      361 gctcatgcga cgagtagtag tacggggtcg ccgcctcgaa ctcggccgcg caggtgtcga
      421 ccgagttgta gatccgctcg aggccgccgg tgccgtcgcc gtcggtggcc agcagttcca
      481 gttcgcgcag gaaccagggg tcgatcttcg cctgttcgtg aacctgctcg acggtcagcc
      541 cgcgctcgaa ggcggcgagg accaggtcga tccggcccgg ggtcggggtg gcgatcgcgt
      601 tcagcagccc gttgtcgctc tccggcactt cgggccggga atcgagctcg cgggaagcca
      661 gcgccttggc gaaggattcg cggaaggtcc tcccgatcga catgacctcg ccgacgctct
      721 gcatgtaggt cgagagcttc tcgtcggctc cggggaactt ctcgaaggcg aagcgcggga
      781 tcttggtcac cacgtaatcg atggtcggct cgaatgaggc cggggtcgcc cgggtgatgt
      841 cgttcgggat ctcctcgagc gcgtagccga ccgccagctt ggccgccatc ttggcgatcg
      901 ggaagccggt tgccttcgag gccaaagccg acgagcgcga cacccgcggg ttcatctcga
      961 tcaccacgat ttcgccgtcg gccgggttga ccgcgaactg gacgttggag ccaccggtct
     1021 cgaccccgac ctcgcgaatc acggcgatcg cgatgtcccg cagcgactgg tagaccgggt
     1081 cggtcagcga ctgggccgga gcgacggtga tcgaatcgcc ggtgtggacc cccatagggt
     1141 c
//
LOCUS       DAOQFI010000765         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_103472_length_1134_cov_2.427247, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000765 DAOQFI010000000
VERSION     DAOQFI010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103472_length_1134_cov_2.427247"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLE93_11280"
     CDS             complement(<1..189)
                     /locus_tag="PLE93_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87675.1"
                     /translation="MPGESTDLPVVQMGAAQIAAAIRAGELTSAEAVEASIDRIEKVN
                     PRLNAVVTPTFESAREAAA"
     gene            complement(192..>1134)
                     /locus_tag="PLE93_11285"
     CDS             complement(192..>1134)
                     /locus_tag="PLE93_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNH87676.1"
                     /translation="LIGEPNRGLAYMFHMMNEARIGVGLGATALGYTGYLKSLRYARE
                     RPQGRNLASKNPEDPQIPISEHTDVKRMLLAQKSYVEGALGLELYCSSLVDDEMTAPD
                     EDDRTRAGLLLEILTPIAKSWPSQWCLEANSLAIQVHGGYGYAREYDVEQHYRDNRLN
                     PIHEGTHGIQALDLLGRKVTMQGGAAFGALFEVITATIDEAKGAGGEAGELADQLAAS
                     LGRLAETTGKLWADRDPELALANATLYLEAAGHIVLAWIWLQQYLAAAGKEGEFYDGK
                     RQATRYFFRFELPKTGPQFDLLASLDRTTLEMEDAWF"
BASE COUNT          167 a          375 c          375 g          217 t
ORIGIN      
        1 ggcggcggct tcccgggcgg attcgaaggt cggggtgacc accgcattca gccgcggatt
       61 gactttttcg atccggtcga tgctcgcctc gaccgcctcc gccgaggtca gctcgccagc
      121 ccggatggcc gcggcgatct gcgccgcgcc catctggact accggtagat cggtgctctc
      181 cccgggcatg actagaacca ggcgtcctcc atctcgaggg tggtccggtc gaggctggcc
      241 agcaggtcga actgcggccc ggtcttcggc agctcgaagc ggaagaagta gcgggtcgcc
      301 tgccgcttgc cgtcgtagaa ctctccttcc ttgccggccg cagcgaggta ctgctgcagc
      361 cagatccagg ccaggacgat gtgcccggcc gcctcgaggt acagggtcgc gttggccagg
      421 gcgagttcag ggtcgcggtc agcccagagc ttgcccgtcg tttcggcgag ccgtcccagt
      481 gaagccgcca gctgatcggc gagctcgcct gcctcgcccc cggccccctt cgcctcgtcg
      541 atcgtcgccg tgatcacttc gaacagcgcg ccgaaggcgg cgccgccctg catcgtcacc
      601 tttcgaccga gcaggtcgag cgcctggatc ccgtgggttc cttcgtggat cggattcagc
      661 cggttgtcgc ggtagtgctg ttcgacgtcg tactcgcgcg cgtagccgta gccaccgtga
      721 acctggattg ccagagaatt ggcttccaga caccactgcg agggccagct cttggcgatc
      781 ggggtgagga tttccagcag cagaccggcc ctggtgcggt cgtcttcatc cggagcggtc
      841 atctcgtcat cgaccagact tgagcagtag agctcgaggc cgagcgcacc ctccacgtag
      901 ctcttctgtg cgagcagcat gcgcttcacg tcggtgtgtt cggagatcgg gatttgcggg
      961 tcttccggat tcttcgaagc gaggttgcgg ccctgggggc gttccctcgc ataacgaagg
     1021 gacttgaggt accccgtgta accgagcgcc gtcgcgccga gaccgacccc gatccgggcc
     1081 tcgttcatca tgtggaacat gtaggcgagg ccccggttcg gctcgccgat cagg
//
LOCUS       DAOQFI010000766         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_103590_length_1133_cov_4.949907, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000766 DAOQFI010000000
VERSION     DAOQFI010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103590_length_1133_cov_4.949907"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..504)
                     /gene="rplQ"
                     /locus_tag="PLE93_11290"
     CDS             complement(151..504)
                     /gene="rplQ"
                     /locus_tag="PLE93_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNH87677.1"
                     /translation="MRHAKKRNKLGRDSAHRKALLSNLSKEIIEHERIKTSQAKAKAA
                     KPEIEKLITLGKRGDLHARRQALSVLRQDKFAVHKLFEEIAPRYAERNGGYTRIVKIG
                     PRKSDSTEMVFLELV"
     gene            complement(534..>1133)
                     /locus_tag="PLE93_11295"
     CDS             complement(534..>1133)
                     /locus_tag="PLE93_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNH87678.1"
                     /translation="DAPIATLEKKTRLEMYVTIGRGRGYRPAEENKDADQPIGVIPID
                     SIFSPIRRASYTVEAARVGQRTDFDKLTLELETDGSIEPNTALREASEILIKSLAIFS
                     DPDRVEELTAREPVVAEGIVADNGAPVATGGDNDILIDELELSVRSYNCLKRAGIETI
                     GQLLAKSESELAAIPNFGSKSIEEVIENLEGRGHHLRQD"
BASE COUNT          187 a          329 c          379 g          238 t
ORIGIN      
        1 taggcgcagt atcagaaaca ccaaataacc ggaaaagaag cgctttcaaa ccggttccac
       61 cggcccgatg gaggttggag caagcgaaag gggagccgat ggctcccctc ttgcggcgac
      121 ttgcctgttc agaacccgtc tgaccggaga tcagacaagt tcgaggaaga ccatttcggt
      181 cgaatcggac ttgcggggac cgatcttgac gatccgggtg tagccgccgt tccgctcggc
      241 gtagcggggg gcgatctcct cgaacagctt gtggacggcg aacttgtcct ggcgcaggac
      301 cgaaagggcc tggcgccggg cgtgaaggtc accgcgcttg ccgagggtga tcagcttctc
      361 gatctccggc ttggcagcct tggccttggc ctggctggtc ttgatccgct cgtgctcgat
      421 gatctccttg gagagattgc tgagcagcgc cttccggtgg gcggaatccc gtcccagctt
      481 gttccttttc tttgcgtggc gcatcgtctc tccttaaggg ttactcggta atgctagtcc
      541 tggcgcaggt gatgcccgcg gccttcgagg ttctcgatga cctcttcaat cgacttcgaa
      601 ccgaagttcg ggatcgcggc aagctcggat tcggacttgg ccagcagctg gccgatcgtc
      661 tcgatgccgg cccgcttgag gcagttgtaa gaacggacgc tgagctcgag ttcgtcgatc
      721 aggatgtcgt tgtcgccgcc ggtggcaacc ggggcgccgt tgtcggcgac gattccttcg
      781 gcaacgaccg gctcgcgggc agtcagctct tcgacccggt ccgggtcact gaagatcgcg
      841 aggctcttga tcaggatctc ggaagcttcg cggagcgccg tgttcggttc gatcgaaccg
      901 tcggtctcca gctcgagggt gagcttgtcg aagtcggtgc gctgaccgac acgggcagcc
      961 tcgacggtgt aggaggcgcg gcggatcggc gagaagatcg agtcgatcgg gatcacgccg
     1021 atcggctgat cggcatcctt gttctcctcg gccgggcggt aaccgcggcc gcggccgatg
     1081 gtcacgtaca tctcgaggcg ggtcttcttc tcgagcgtgg cgatcggagc gtc
//
LOCUS       DAOQFI010000767         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_103842_length_1132_cov_2.482823, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000767 DAOQFI010000000
VERSION     DAOQFI010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103842_length_1132_cov_2.482823"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLE93_11300"
     CDS             complement(<1..1084)
                     /locus_tag="PLE93_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNH87679.1"
                     /translation="MEDVPSSVLESVSDRLQATVDYADLADCDAIIICVPTPLTGSSE
                     PDLTYLESAARGLASILVPGQLVVLESTTYPGTTRDLLAGQLEAGGLIAGRDFHLAFS
                     PERIDPGRTDYTVKTTPKLVGGITPECTDRAASLYETVCDRVVRLSGPEAAELSKLLE
                     NIFRSVNIALVNELAQLCDRLDVDVWEVVEAASTKPFGFMPFEPGPGMGGHCLPIDPF
                     YLAFTARQNDFYPEFIELAGKINESQPRFCVSRIERILNGEGKPVKGSKILILGVSYK
                     AGIGDIRESPALKIIRLLGELGADLSYHDPHVPELDEFGLKHTESLEPALENADLAVI
                     VTNHPEVSLDTVVGKSQLLLDLRGVTR"
BASE COUNT          226 a          327 c          344 g          235 t
ORIGIN      
        1 tccgggtcac gccgcgcagg tcgagcaaca attggctctt gccaaccacc gtgtcgaggc
       61 tgacctcggg atggttggtg acgattaccg ccaagtcggc attttcgagg gccggctcaa
      121 ggctttcggt gtgcttcagg ccgaattcgt ccaactccgg cacatgcggg tcgtggtagc
      181 tcagatcggc accgagctct ccaagtagcc ggatgatctt cagcgccggc gattcgcgga
      241 tgtccccgat accggccttg tagctgactc cgaggatcag gatcttggat cccttgaccg
      301 gcttgccttc accgttcagg atccgctcga tccgggacac gcagaagcgc ggctgagact
      361 cgttgatctt gcccgccagt tcgatgaact cggggtagaa gtcattctgc cgggcggtga
      421 atgccagata aaagggatcg atcggaagac aatgtccgcc catgcccgga ccgggctcga
      481 acggcatgaa cccgaatggc ttggtggagg ccgcttccac gacttcccaa acatctacgt
      541 cgagtcggtc gcagagctgg gcaagctcat tgacgagagc tatgttgacc gagcgaaaga
      601 tgttttccag cagttttgag agctctgccg cttccggacc cgaaagcctg accacccgat
      661 cgcaaaccgt ttcgtagagg gacgctgcgc gatcagtgca ctcgggtgtt attccgccga
      721 caagcttcgg cgtggtcttg accgtgtagt cggtccgccc cggatcgatc cgttccggcg
      781 aaaatgcgag gtggaagtcc cggccggcga tcaacccgcc tgcttccaac tggccagcca
      841 acaggtccct tgtcgttcca gggtaggtcg tcgattcgag cactacgagc tgaccgggaa
      901 ctagaatcga agccagtcca cgggcggcgg actccaagta ggtcaggtcc ggttcgctcg
      961 aaccggtaag tggtgtggga acgcagataa tgatcgcgtc gcaatcggcc agatccgcgt
     1021 agtcaactgt tgcctggagt cgatcactga cactttcgag taccgacgaa ggaacatcct
     1081 cgatgtaggt cttcgactga ttgagacttg cgactttgga cggattggcg tc
//
LOCUS       DAOQFI010000768         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_103964_length_1131_cov_4.150558, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000768 DAOQFI010000000
VERSION     DAOQFI010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103964_length_1131_cov_4.150558"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..731)
                     /locus_tag="PLE93_11305"
     CDS             complement(6..731)
                     /locus_tag="PLE93_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH87680.1"
                     /translation="MADVTSIETGTEKVRLDFPAEAVARLKISNPPKRNALDHEILDA
                     ITATVPRLDQGIEVRCLVITGEDPVFSAGYDIGGIPAESFETDAEALVAHPFTAALDA
                     IEDFPWPTVAALNGHCLGGALEMAVTCDLRIAAPHAKLGMPPAKLGLIYGHTGLIRFI
                     RTIGLPRTRELFFTGDMLPAGRADEIGLVNRVTAGDEDFAVEAVEFAAGIAASAMPSP
                     PPSTRMRRAPGNAARPGWTRASW"
     gene            complement(756..989)
                     /locus_tag="PLE93_11310"
     CDS             complement(756..989)
                     /locus_tag="PLE93_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNH87681.1"
                     /translation="MAIKFRLMANIEAHITGNVWKIQVKVGDEVSEEDEVIILESMKM
                     EIPVESEDDGVVKEILCEEGQSVTEGDVLIVLE"
BASE COUNT          179 a          372 c          381 g          199 t
ORIGIN      
        1 taccgctacc acgaggccct ggtccatcct ggcctcgcgg cattgcccgg cgcgcgccgc
       61 atcctggtgc tgggcggcgg cgacggcatg gcgctggcgg cgatgccggc cgcgaattcg
      121 accgcctcga ccgcgaaatc ctcgtccccg gcggtaaccc ggttgaccag accgatttcg
      181 tcggcccggc cggcgggcag catgtcaccg gtgaagaaaa gctcgcgggt gcggggcagg
      241 ccgatggtgc ggatgaagcg gatcaggccg gtgtggccgt agatcagacc gagcttggcc
      301 ggcggcatgc cgagcttcgc gtgcggggcg gcgatcctca ggtcacaggt gaccgccatc
      361 tccagcgctc ctccgaggca gtgaccgttc agggcggcga cggtcggcca gggaaagtcc
      421 tcgatcgcgt cgagggcggc ggtgaatggg tgggcgacga gagcctcggc gtcggtttcg
      481 aagctctcgg ccgggatgcc gccgatgtca taccccgccg agaagaccgg gtcctcgccg
      541 gtgatgacca ggcaacggac ctcgatgcct tgatcgagcc ggggcaccgt ggccgtgatc
      601 gcgtcgagaa tctcgtggtc gagcgcgttc cgcttgggcg ggttcgaaat cttcaggcgg
      661 gcgaccgctt ccgcggggaa gtcaagccgg accttctccg tgccggtttc gatcgaggtc
      721 acgtcagcca tcggcgtcgg ctcgccgggg gacggctact cgaggacgat caggacgtcg
      781 ccttcggtca cggactggcc ctcttcacag aggatctcct tgaccacgcc gtcgtcctcg
      841 gactcgaccg ggatctccat cttcatcgac tcgaggatga tcacttcgtc ttcttcggag
      901 acttcgtccc cgaccttgac ctggatcttc cagacgttcc cggtgatgtg agcttcgatg
      961 ttcgccatga gccggaactt tatcgccacg gccatccaca gaggtcgcgg cctcaacctc
     1021 tagagtccgc ccacggtgga gagttcgaag accaactcac tggcggtcct gatcgcggca
     1081 cgaaacgagg ccgacgtgat cgccgccacg atcggctctc tccgcgaagc c
//
LOCUS       DAOQFI010000769         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_104184_length_1130_cov_2.720930, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000769 DAOQFI010000000
VERSION     DAOQFI010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104184_length_1130_cov_2.720930"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLE93_11315"
     CDS             <1..690
                     /locus_tag="PLE93_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH87682.1"
                     /translation="LDLEIPDGSFFGLLGPNGAGKTTLIGAVCNLIRVTGGEISVFGR
                     PGTGMAARRMIGIAEQEPNLDKFLTVYETLLYHGGYYGMDGREASERAEQMIDAFDLR
                     AKRDVEAPKLSGGMRRRLLLARALLHRPRLVILDEPTAGVDLELRHELWRYIRGLHEQ
                     GTTILLTTHYLEEAEALCDEIALISGGHIVARDSAEGLKRSHGVETLEEVYLKTVAGG
                     RIAMDQLEEVE"
     gene            687..>1130
                     /locus_tag="PLE93_11320"
     CDS             687..>1130
                     /locus_tag="PLE93_11320"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH87683.1"
                     /translation="MSNAASGVGYIGLGIMGRPCALNLIAAGYQLTVNTRTRSKAGAV
                     LDAGAAWADTPAGVAANSEVVFLNLPDTPDVEAVIFGKDGITSAAGFNGVVVDMSTID
                     PGRTTEIAAELAGGGIGFVDAPVSGGEKGAIEGNLSVMAGGSETDF"
BASE COUNT          193 a          378 c          390 g          169 t
ORIGIN      
        1 ctcgacctcg agatccccga cggcagcttc ttcgggctgc tcgggccgaa cggagccggc
       61 aagacgacgc tgatcggggc ggtctgcaac ctgatccgcg tgaccggcgg cgagatctcg
      121 gttttcggcc ggcccggcac cggcatggcc gcccgccgga tgatcggcat cgccgagcag
      181 gagccgaacc tcgacaagtt cctcaccgtt tacgagaccc tgctctacca cggcggctac
      241 tacggcatgg acggtcgcga ggcgagtgaa cgggccgagc agatgatcga cgccttcgac
      301 ctgcgagcca aacgggacgt ggaagcgccg aagctctccg gtggcatgcg tcgccgcctg
      361 ctgctggccc gggccctgct tcaccggccc cggctggtca tcctcgacga gccgaccgcc
      421 ggcgtggacc tggaattgcg gcatgagctc tggcgctaca tccggggcct tcacgagcag
      481 ggcaccacga tcctcctgac cacccactac ctggaggaag ccgaggcgct ctgcgacgag
      541 atcgccctga tcagcggcgg tcacatcgtc gcccgggatt cagccgaagg gctgaagcgc
      601 tcgcatgggg tcgaaaccct cgaagaggtc tacctgaaga cggtcgccgg gggccggatc
      661 gccatggatc aactggagga agtcgaatga gcaacgcagc ttccggagtc gggtacatcg
      721 ggctgggcat catgggtcgg ccctgtgccc tgaacctgat cgcggccgga taccagctga
      781 ccgtgaacac ccgcacccga tcgaaggccg gcgcggtcct ggacgccggg gccgcctggg
      841 cggacactcc ggccggggtt gccgcgaaca gtgaggtcgt cttcctcaac ctccccgaca
      901 ccccggacgt cgaggcggtg atcttcggca aggacgggat cacctcggcc gccggcttca
      961 acggagtcgt ggttgacatg agcacgatcg accccggccg caccaccgag atcgcggccg
     1021 agctggcggg tggcggcatc ggattcgtcg acgctcccgt ttccgggggc gagaagggcg
     1081 cgatcgaggg gaacctttcg gtgatggcgg gtggcagcga gaccgacttc
//
LOCUS       DAOQFI010000770         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_104776_length_1127_cov_2.090485, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000770 DAOQFI010000000
VERSION     DAOQFI010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104776_length_1127_cov_2.090485"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLE93_11325"
     CDS             complement(<1..296)
                     /locus_tag="PLE93_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87684.1"
                     /translation="MRTHWTSSMVLPDVNVLLRAFRPELQAHEVSAAWLQNQIEGPAP
                     FAMADRVIEGFVRIVTHPGGFDRPEPMPSALDFVEMLLSLENCVRVNPGPLQWE"
     gene            complement(253..522)
                     /locus_tag="PLE93_11330"
     CDS             complement(253..522)
                     /locus_tag="PLE93_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNH87685.1"
                     /translation="MRTTVRLDEQLMRDAKALAAKEGKTFTALIEDSLRERLARSRNW
                     ESRKRRNFPVSKMSGGLREGLDPEILSDNSKLADALDEFDGFAGR"
     gene            648..>1127
                     /locus_tag="PLE93_11335"
     CDS             648..>1127
                     /locus_tag="PLE93_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87686.1"
                     /translation="MTVSEAPTDPQEPEGAAAPATDPTGLTSAEAARRLEERGPLPKP
                     ESSRSYRSIVVGNIFTVFNAILLAFGLLTIFFGNPKDALFMGILVANATIGIGQEVRA
                     KLALDKLAALIAVKAEVMRDGQKVEIPVDDVVVGDLVLIGPGDQVIADGEILTADGLQ
                     "
BASE COUNT          211 a          371 c          332 g          213 t
ORIGIN      
        1 atttcccact gaagcggtcc tgggttcacc ctgacgcagt tctccagact gagaagcatc
       61 tcgacaaaat ccagcgcgct gggcataggt tcaggacggt cgaacccacc cgggtgggtc
      121 acgatccgga cgaacccctc aatgactcgg tcggccatcg cgaaaggtgc cggcccttcg
      181 atctggttct ggagccaggc tgccgagact tcatgggcct gaagctcggg gcgaaacgcc
      241 ctcagcagga cgttaacgtc cggcaaaacc atcgaactcg tccagtgcgt ccgcaagctt
      301 ggaattgtcg ctcaggatct ccgggtccag accctcgcgc agtcctccag acatcttcga
      361 tacggggaaa tttcgacgct tgcgtgattc ccaatttcgt gacctcgcga gtcgctcgcg
      421 gagagaatcc tcgatcagtg ccgtaaaggt ctttccttcc ttggcggcaa gggccttcgc
      481 atcccgcatc aactgctcgt caagtctcac ggtggtcctc atgctttcca gattcccact
      541 ttgacataaa tatgtcaagt gaacgccttc tcgatcccat ggcccattca gtgcccggaa
      601 ccggaaactc gccccctctc gggccgggca aatagagtcc gggcagcttg accgtctctg
      661 aggccccgac cgacccccag gaacctgaag gcgcagccgc gccggcgacc gacccgacag
      721 gactgacctc ggccgaggcc gcccggcgcc tcgaggagcg cgggcccctt ccgaagcctg
      781 aatcgagccg gtcttaccgg tcgatcgtgg tcggcaacat cttcacggtc ttcaacgcga
      841 tcctgctcgc cttcggcctg ttgacgatct tcttcggcaa tcccaaggac gcgcttttca
      901 tggggatcct ggtcgccaac gcgacgatcg ggatcggcca ggaggtccgt gcgaagctgg
      961 ccctcgacaa gctcgccgcc ctgatcgcgg tcaaggccga ggtgatgcgg gacggccaga
     1021 aggtcgagat cccggtcgac gatgtggtgg tcggggacct ggtcctgatc gggccgggtg
     1081 accaggtgat cgccgacggt gagatcctca ccgcagacgg cctccag
//
LOCUS       DAOQFI010000771         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_107143_length_1114_cov_2.497639, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000771 DAOQFI010000000
VERSION     DAOQFI010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107143_length_1114_cov_2.497639"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLE93_11340"
     CDS             <1..597
                     /locus_tag="PLE93_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87687.1"
                     /translation="PQGIQDVAIPVLSNDSATARFTLSIPGPPLEVEKPGGGTMIVKG
                     QDPNYARAKCSTGEWVTGGTFPLGERAFPAGTDTGPEIELEATPYETTCDGKSGQARL
                     GNAKASGPKLLKRGRSRIFRVSVRNNGTATARDIKVTVSGSGKGQARAANIAPGKSLT
                     IPVKVKATGPKGKPAKLAFRISGQGTRTRAGFRGRIAG"
     gene            752..>1114
                     /locus_tag="PLE93_11345"
     CDS             752..>1114
                     /locus_tag="PLE93_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87688.1"
                     /translation="MKKYIALVIAMIVPFALVANANAANETVTATTTLAPQGGGNYFK
                     EVTSSSQLTIRAEVNTPSDSPKVNPMKNVKITVPERHDLQAEQLEDAGLHRQQAEHHL
                     EPGRSRGRRRFLPQVGGWF"
BASE COUNT          245 a          368 c          333 g          168 t
ORIGIN      
        1 ccgcagggca tccaggatgt cgccatcccg gttctcagca acgactcggc gactgcgaga
       61 ttcaccctga gcattcccgg gccgccactc gaggtggaaa agccgggcgg cgggacgatg
      121 atcgtgaaag gccaggaccc gaactatgcc cgggcgaaat gctcgaccgg cgagtgggtc
      181 accggcggca ctttccccct gggtgagcgg gcctttcccg ccggcaccga caccggaccg
      241 gagatcgaac tggaagcgac cccgtacgaa acgacctgtg acggcaagtc cggccaggcc
      301 aggcttggca acgcgaaggc cagcgggccg aagcttctga agcggggaag aagcaggatc
      361 ttccgcgtca gcgtacgcaa caacggaacc gcaactgcca gagacataaa ggtgaccgtt
      421 tcgggaagcg gcaagggcca ggcccgggcc gcgaacatcg ctcccggcaa gtcactgacg
      481 attccggtca aggtcaaggc gaccggtccg aaaggcaaac cggcgaagct cgctttccgg
      541 atctccggcc agggcaccag gacccgggcc gggttccggg gccgaatcgc cggctgaccc
      601 cccgaagccg cctggcgggt tcgcaattaa ggttgggtaa acggcgaaac aaagccgtct
      661 ggcgagggta tttacttgtg acggaccgca aatttcggcg gcccgtctcg ttctctccaa
      721 cccacctgat tgaggcccac caatgaatcc catgaagaaa tacatagccc tcgtgatcgc
      781 catgatcgtt cccttcgcgc tggtagcgaa tgccaacgcg gcgaacgaga ccgtgaccgc
      841 gaccaccacc ctcgcccctc agggcggcgg caactacttc aaggaagtca cttcgtcttc
      901 ccagctcacg attcgagccg aagtaaacac tccgtccgat tctccgaagg tcaacccgat
      961 gaagaacgtg aagatcaccg ttcccgaacg gcatgacctt caagccgaac aactcgaaga
     1021 cgccggcctg caccgacagc aagctgagca tcacctcgaa cctggccgat cccgcgggcg
     1081 tcgtcgcttc ctgccccaag tcggtggttg gttc
//
LOCUS       DAOQFI010000772         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_107266_length_1113_cov_3.532136, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000772 DAOQFI010000000
VERSION     DAOQFI010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107266_length_1113_cov_3.532136"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLE93_11350"
     CDS             complement(<1..291)
                     /locus_tag="PLE93_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNH87689.1"
                     /translation="MAERRGMGRGLGAILPESATGPDMRELPVKSIDRNPEQPRTNFS
                     EEALEALAESIRASGVVQPLIVRPAGRGKYEIIAGERRWRAAQMAGVEKVPVV"
     gene            complement(284..>1113)
                     /locus_tag="PLE93_11355"
     CDS             complement(284..>1113)
                     /locus_tag="PLE93_11355"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNH87690.1"
                     /translation="PLHEAFFVHVRIPSCPDLFGDRGIQLIYSVANQKGGVGKTTTAV
                     NLGAAYAQLGKRVLVVDLDQQCNATVAFGLEKQDSPTTYEVLSGEVTLSEAAKPAGPE
                     NVWMVPASRDLAGAVVELPRLERPNHRLQEQVGSVDEDFDVVFIDCPPALGPITVNSL
                     VASDRVIVPVQAEYLALEGLVEFLDTLSTVRREMNPTLELAGVLVTMHDERTRLAQDV
                     ERELRDHFSETVFRTVIPRTVRVAEAPSYGIPVTDYSPSSRGSEAYLALAEEVMARG"
BASE COUNT          189 a          375 c          358 g          191 t
ORIGIN      
        1 taccaccggg actttttcga ccccggccat ctgtgcggcc cgccagcgcc gctcgccggc
       61 gatgatctcg tactttccgc gcccagccgg ccgcacgatg agtggttgga ccactccact
      121 ggcccggatc gattcggcga gggcctcgag agcctcctcg gaaaagttgg tccggggctg
      181 ctccggattg cggtcgatcg actttaccgg cagctcgcgc atgtcaggcc cggtggcgct
      241 ttccggcagg atcgccccga ggccccgccc cattccgcgc cgctcagcca cgtgccatca
      301 cctcttcggc cagggcaaga taggcctcgg aaccccttga cgaaggggaa tagtcggtca
      361 ccgggatgcc gtaactcggg gcctccgcga cgcgcacggt ccttggtatc accgtccgga
      421 acacagtctc ggagaagtga tcgcgaagct cacgctcgac atcctgggca agtctggtcc
      481 gctcatcatg catggtgacg aggacgccag ccagctccag ggtcgggttc atctcccgcc
      541 gcacggtcga aagggtgtcg agaaactcga ccaggccttc gagcgccagg tactcggcct
      601 ggaccggcac gatcacccgg tcggaggcga ccagcgagtt gacggtgatc ggtccgagcg
      661 cgggcgggca gtcgatgaag accacgtcga agtcctcgtc gaccgatccg acctgctcct
      721 gcagccggtg attcggtcgt tcgagccggg gcagttcaac caccgctccg gccaggtcac
      781 ggctggccgg gaccatccag acgttctccg gaccggccgg cttggccgcc tcggagaggg
      841 tcacctcgcc gctcagcacc tcataggtgg tgggtgaatc ctgcttctcg aggccgaagg
      901 cgaccgtcgc gttgcattgc tggtcgagat cgacgaccag cacccgcttg cccagctggg
      961 cgtaggccgc cccgaggttg acggcagtgg tggtcttgcc gactcctccc ttctggttgg
     1021 cgaccgaata gatcaactga atgccccggt caccgaagag gtccggacag gaaggaatcc
     1081 gcacgtggac gaagaatgcc tcatgtagag gcc
//
LOCUS       DAOQFI010000773         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_107353_length_1113_cov_2.224953, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000773 DAOQFI010000000
VERSION     DAOQFI010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107353_length_1113_cov_2.224953"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLE93_11360"
     CDS             complement(<1..468)
                     /locus_tag="PLE93_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87691.1"
                     /translation="MDRPEPQTEEEFEREFRRAGLPLLNENYSASEDIFNRAVPLLGL
                     VLVLEVTGPLNKSWSTLGNVAAVATAMLLMLVALGLMNRMRGRAFFSLPRKVGRPELA
                     AFVIVPALLPGIISGHWGIAALTALFNLALLLVILGVVGFGVISIIHWALARLL"
     gene            509..1045
                     /locus_tag="PLE93_11365"
     CDS             509..1045
                     /locus_tag="PLE93_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNH87692.1"
                     /translation="MKQLDLKLLRIMRTRGHAAPVEKSAHLISKSGENGYLWFGIGLA
                     GAVFDGEHRKEWLLASLLGPVAIGLNFLVKLVVRRPRPELEGLPPLGGAPSSLSFPSA
                     HATASFAAATAMSRIRPELKLALFGAAAVMAVSRPYLGMHYPSDIAAGAVLGTVLGTA
                     AGNFLAGPEEDENSPYNF"
BASE COUNT          199 a          361 c          359 g          194 t
ORIGIN      
        1 gagcaggcgg gccagggccc agtggatgat cgagatcacg ccgaaaccga cgactccgag
       61 gatgacgagg agcagggcaa ggttgaagag ggccgtcagg gccgcgattc cccagtggcc
      121 ggaaatgatt cccggcagga gggccggcac gattacgaag gcggcgagct cggggcgtcc
      181 cactttgcgc ggcagcgaga agaaggcgcg gccgcgcatc cggttcatca ggccgagagc
      241 gaccagcatc aacagcatcg cggtcgccac cgcggcgacg ttgccaaggg tcgaccagct
      301 cttgttcaga ggtccggtca cttccaggac caggaccagt ccaagcagcg gcacggcccg
      361 gttgaagatg tcctccgaag ccgagtaatt ctcgttcagg agcggcaggc cggcgcggcg
      421 gaattcccgc tcgaactctt cttcagtctg gggttcggga cgatccacgc cgcgatcatc
      481 ccgaatggcc ccctattctg ctcacttcgt gaagcaactg gatttgaaac tgctgcgcat
      541 catgcgcacc cgcggtcacg ccgctccggt cgagaagtcg gctcacctga tctcgaagtc
      601 gggcgagaac ggctacctct ggttcgggat cggcctggcc ggcgcggttt ttgacggaga
      661 gcaccgcaag gagtggctgc ttgcttcgct gctcggcccg gtggcgatcg gccttaactt
      721 cctggtcaag ctggtggtaa ggcgtccgcg gccggagctt gaaggcctgc ccccgctggg
      781 cggtgcgccc tcttcgcttt cgttcccctc cgcccatgcc acggcttcgt ttgccgcggc
      841 tacggcgatg agccggatcc gcccggaatt gaaactcgcc ctcttcggag ccgccgccgt
      901 gatggcggtc agccgtccct acctcggcat gcactacccc agcgacatcg ccgctggggc
      961 ggttcttggc acagtcctcg gaaccgccgc cggaaacttc ctcgccggcc ccgaagaaga
     1021 cgaaaactcg ccttacaact tctagccacg gatcaccgaa tgcccacttt gtgccggtat
     1081 gtggcgcaaa gtgggatgtc gatgaatccg agg
//
LOCUS       DAOQFI010000774         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_107430_length_1113_cov_1.459357, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000774 DAOQFI010000000
VERSION     DAOQFI010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107430_length_1113_cov_1.459357"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLE93_11370"
     CDS             <1..604
                     /locus_tag="PLE93_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNH87693.1"
                     /translation="AAARQGNGLIEITARGSLQIRGLTAQSAVELAAEVDGLGIAVRT
                     GVPVETPPLAGLDTAEVVDPRPLAHRIREAIAAAGLEGRRFDLAIDMVGGETLQAMLR
                     QLRPGGRAVIYGVAGGEPAKVNTGALLKNGLTVSGLWLGYSEGWSLREIMAGLFELVE
                     TGKLAPTLGPTFPLARAGEAHRAVEARTGVGKVTLDCRNP"
     gene            complement(651..>1113)
                     /locus_tag="PLE93_11375"
     CDS             complement(651..>1113)
                     /locus_tag="PLE93_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HNH87694.1"
                     /translation="VFVRSPYLFEGYFDESGIQSPIGPDGFMTVGDVGVLDQRGLSLV
                     GRASNMLITGGKNVHPEEVEALLAEHESVTECVVVGVPDPRWGEEIVAFVVPADRSSG
                     PEVAELREHLKAGLASYKVPKRWFTVSEIPRTRAGKTDRSPERLFQSATGI"
BASE COUNT          181 a          380 c          388 g          164 t
ORIGIN      
        1 agcggcggcg cggcagggta acgggctgat cgagatcacg gcgcgcggca gcctgcagat
       61 acgcggactg acggcgcagt ccgcggttga gcttgccgcc gaggtcgatg ggttgggcat
      121 cgcggtacga actggtgtgc cggtggaaac gccgccgctc gccgggctgg atacggccga
      181 ggtggtcgac ccgcgcccac tcgcccaccg cattcgcgag gcaatagccg cggcggggct
      241 ggaaggccgc aggttcgacc tcgcgatcga catggtcggc ggcgagacgc ttcaggcgat
      301 gctgcggcag ctccgtcccg gaggccgggc ggtgatctac ggggtggccg ggggagagcc
      361 ggcaaaggtc aacaccgggg cgctgctgaa gaacggcctt accgtctccg ggctctggct
      421 gggctactcg gaaggctggt cactgcggga aatcatggcc gggctcttcg agctggtcga
      481 aaccggcaag ctggctccga ccctgggccc gacctttccg ctcgcccggg ccggcgaggc
      541 ccaccgggcg gtcgaggccc ggaccggcgt cggcaaggtc actctcgact gtcgcaaccc
      601 ctgactgccg gaaccggcca gccgcctgaa ccagccggag gtggccggac tcagatcccg
      661 gtcgccgact gaaagagccg ctcgggggag cggtcggtct tgccggcccg ggtccgcggg
      721 atctcactga cggtgaacca gcgtttcggc accttgtacg aagccagccc cgctttcagg
      781 tgttctctca gctctgccac ctcggggccg gaactccggt ccgcggggac cacgaaagcc
      841 acgatctctt cgccccagcg gggatcggga accccgacca ccacgcattc ggtgaccgac
      901 tcatgttcgg ccagcagggc ctcgacctcc tccgggtgca cgttcttgcc accggtgatc
      961 agcatgttcg aggcccgacc gaccagtgac agccctcgct ggtcgagcac cccgacgtcg
     1021 ccgaccgtca tgaaaccgtc cggcccgatc ggcgactgga ttcccgactc gtcgaagtaa
     1081 ccctcaaaga gatacgggct gcggacgaag acc
//
LOCUS       DAOQFI010000775         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_107462_length_1112_cov_3.871334, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000775 DAOQFI010000000
VERSION     DAOQFI010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107462_length_1112_cov_3.871334"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>1112
                     /locus_tag="PLE93_11380"
     CDS             78..>1112
                     /locus_tag="PLE93_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNH87695.1"
                     /translation="MPFAQAAKVPAGAGSGAVAPIAHKGRWFVDATGRVVQLRGVNEV
                     YKTAPYYPAADGFGADDVNLLSKLGLNVVRLGVDLRGLMPTPGKIETAYIDKLAQTVN
                     QLTKAGIYTQVDIHQDGYAPKYNGNGFPDWMAIDDGLPNPDLPFPVYYFGNTAMQRAW
                     DHFWDNSEVNGKGLQEYVIQSLTALVKKFRGNPHVVGYDVFNEPWPGTDFAPCAEATG
                     CPQLERELMMPFARKAVKATRKLTKTQPVFVEPFLFFNLGRPTVLPGSTGAWLSAHNY
                     ATDENDALMVKETVKAATRDQVPAILTEFGATSDNATIDRLTSIYDARIVSWIFWAYN
                     ENIVTNGKVPA"
BASE COUNT          217 a          379 c          334 g          182 t
ORIGIN      
        1 cgactactgt ttgcactctc acgaacccga ttcgcgctgg tctagccacc ctggccgtag
       61 ccgcatcgct cgtcgcggtg ccgttcgcac aggccgccaa ggttcccgcc ggggcaggtt
      121 ccggcgcggt tgccccgatc gctcacaaag gccgctggtt cgtggatgcg accgggcggg
      181 tcgtgcagct ccgcggggtc aacgaggtct acaagacggc gccgtattac ccggcggcag
      241 acggattcgg agccgatgac gtcaacctgc tgagcaagct cggactcaac gtggttcggc
      301 tgggggtcga cctgcgtggc ctcatgccga cccccggcaa gatcgagacc gcctacatcg
      361 acaagctggc gcagacggtc aaccagttga ccaaggccgg gatctacacc caggtcgaca
      421 tccaccagga cggttacgcc cccaaataca acggcaacgg ctttcccgac tggatggcaa
      481 tcgacgacgg cctgccgaac ccggatctgc cattccccgt ctactacttc ggcaacaccg
      541 ccatgcagcg ggcctgggat cacttctggg acaactcgga ggtcaacggg aagggcctgc
      601 aggaatacgt gatccagagc cttaccgccc tggtcaagaa gttccggggc aacccccatg
      661 tcgtcggtta cgacgtcttc aacgagccgt ggcccgggac cgacttcgcg ccctgcgccg
      721 aagcaaccgg ctgcccgcaa ctcgagcgcg aactgatgat gcccttcgcc cgcaaggcag
      781 tcaaggcgac ccgcaagttg acgaagaccc agccggtgtt tgtcgagccg ttcctcttct
      841 tcaacctcgg ccgtccgacc gttttgccgg ggtcaaccgg cgcctggcta tcggcccaca
      901 actatgcgac cgacgaaaac gacgccctga tggttaagga gacggtcaag gcggccaccc
      961 gggaccaggt tccggcgatc ctgaccgagt tcggcgcgac cagcgataac gcgacgatcg
     1021 accggctgac cagcatctac gatgcccgga tcgtctcctg gatcttctgg gcctacaacg
     1081 agaacatcgt caccaacggg aaggtgccgg cg
//
LOCUS       DAOQFI010000776         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_108713_length_1106_cov_1.870599, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000776 DAOQFI010000000
VERSION     DAOQFI010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108713_length_1106_cov_1.870599"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1106
                     /locus_tag="PLE93_11385"
     CDS             <1..>1106
                     /locus_tag="PLE93_11385"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNH87696.1"
                     /translation="LIPERLEGIGAGTVLEVPFGRRRITGVVVEVAETSEVPPGKLAE
                     PIRVLGSGTNPDLIELGRWIASEYCSTPARGLGLVLPPGTGTGGERTRFRVEKLNSIT
                     EAGREALDGGARLGSKQKLALQQLLEGDLNGRQLAARAGIDSATLKRLEERGLIERQQ
                     VHVRRRPDGAGVGSAATSAPDLTGAQAAAVARLVANLDQPSAGEGVLLNGVTGSGKTE
                     VYLAVVEEVLARGRTAIVLVPEIGLTPQAVGRFQARLGDRVAVLHSALTAGQRFDEWQ
                     RLKAGDATVCVGPRSAVFAPLENLGLIVIDEEHDPSYKQEGDPRYDAREVARKRALDT
                     GSLLVLGTATPRPESWDRLVRIDLPDRVDGQAMP"
BASE COUNT          164 a          383 c          404 g          155 t
ORIGIN      
        1 acctgattcc ggaaaggctg gaggggatcg gcgcgggcac ggtgctggaa gtgcctttcg
       61 gccggcgccg catcaccggt gtggtggtgg aggtggcgga gacctccgag gtgccgcccg
      121 ggaagctggc cgagccgatc cgggtcctgg gatccggcac caatccggat ctgatcgagc
      181 tcggccgctg gatcgcctcc gagtactgct cgaccccggc ccgcggactc ggcctggtgc
      241 tgccgcccgg caccggtacc ggcggtgaac ggacccgctt ccgggtcgag aaactgaact
      301 cgatcaccga ggcgggccgg gaagcactgg acggtggcgc ccgcctcggc tccaaacaga
      361 agctggccct tcagcaactg ctcgagggtg acctgaacgg ccgccagctg gccgcccggg
      421 cgggcataga ttccgcgacc ctcaagcgcc ttgaggaacg gggcctgatt gagaggcagc
      481 aggttcacgt gcggcgacgg cccgacgggg ccggggtcgg cagcgcggcg accagcgccc
      541 ccgacctgac cggggcacag gcggccgcgg tcgccaggct ggtcgcaaac cttgatcaac
      601 cttccgccgg ggagggagtg ttgctgaacg gggtgaccgg ttccggcaag accgaggtct
      661 acctcgccgt ggtcgaggag gttctggccc ggggccggac cgcgatcgtg ctggtgccgg
      721 aaatcggcct gaccccgcag gccgtaggcc gcttccaggc ccggctcggt gaccgggtcg
      781 cggtccttca ctccgccctc accgccggcc agcgctttga cgagtggcag cgcctgaagg
      841 cgggcgacgc caccgtctgc gtcggcccgc ggtcggccgt cttcgctccg ctggagaacc
      901 ttggcctgat cgtgatcgac gaggaacacg atccctctta caagcaggaa ggcgaccccc
      961 gttacgacgc ccgcgaggtg gcccgcaagc gggcgctcga cacgggttcc ctgctagtgc
     1021 tcggcaccgc caccccccgg cccgagagct gggatcgcct ggtccggatc gacctgccgg
     1081 accgggtcga cggccaggcg atgccg
//
LOCUS       DAOQFI010000777         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_108828_length_1105_cov_2.700000, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000777 DAOQFI010000000
VERSION     DAOQFI010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108828_length_1105_cov_2.700000"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1010
                     /locus_tag="PLE93_11390"
     CDS             27..1010
                     /locus_tag="PLE93_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNH87697.1"
                     /translation="MRAMVLESQPGDLVPRELPVPEPGPGQILIRVSTCGVCRTDLHI
                     VDGELSEPKLPLVPGHQIVGTVEAAGEDADRFGTGDRIGVPWLGWTDGDCRFCREGRE
                     NLCENARFTGYDSDGGYAEFAVADQRFCFPLSAAIDDTHVAPLLCAGMIGYRALAIAG
                     DGERLGLYGFGSSAHILCQITAAEGRRVFAFTRPGDQAGQAFARSLGAEWAGGSDETP
                     PEPFDAAIVFAPVGSLMVDALRASERGARIVSAGIHMSDIPSFPYELLWEERSLHSVA
                     NLTRADGTALLELAARHRVEPMVELLPLESANQALDGIRSGASEGSTVLTV"
BASE COUNT          154 a          375 c          393 g          183 t
ORIGIN      
        1 cggcctgaat cgggaggggc cgggcgatgc gcgcgatggt cctcgaaagc cagcccggcg
       61 acctcgtccc gcgggaactt cccgtccccg agcccggtcc cggccagatt ctgatccggg
      121 tttcaacctg cggggtttgc cggaccgacc tgcacatcgt tgacggcgag ctgagcgagc
      181 cgaagctgcc gctggttccc ggccaccaga tcgtcggcac ggtcgaggcc gcgggagaag
      241 acgcggaccg cttcgggacc ggtgaccgga tcggcgtgcc ctggctgggc tggaccgacg
      301 gggactgtcg cttctgccgg gagggccggg agaacctctg tgagaacgcc cgcttcaccg
      361 gttatgactc cgacggcggg tacgcggagt tcgcggtcgc cgaccagagg ttctgtttcc
      421 ccctttccgc ggcgatcgat gacactcacg tggcaccgct cctttgcgcc gggatgatcg
      481 gctaccgggc gctggcgatc gccggagacg gcgagcgact gggtctctac ggtttcggat
      541 cctccgctca catcctctgc cagatcacgg ccgccgaggg ccgccgcgtt ttcgctttca
      601 ccagacccgg tgaccaggcc ggccaggcat ttgcccgctc gctcggggcc gagtgggccg
      661 gggggtcgga cgagactccg ccggagccgt tcgacgcggc gatcgtcttc gccccggtcg
      721 gttcgctgat ggtcgatgcc ctccgggcca gcgagcgcgg ggcgaggatc gtcagcgccg
      781 gcatccacat gagcgacatc ccctcgttcc cctacgagct gctctgggag gagcggagcc
      841 ttcattcggt ggcgaacctg acccgggccg acggcaccgc cctgctggag ctcgcggccc
      901 ggcaccgggt cgagccgatg gtcgagctgc ttcccctcga aagtgcgaat caggcgctgg
      961 acgggatccg gagcggcgcc tcggaaggtt ccaccgtact caccgtttga cggtgtactc
     1021 gagcgcgacg gtgccgcccg ggaagcgctg gagatcggtc agttcaaggt cccgccgggt
     1081 tccgttcccg gggaagaacg gcttg
//
LOCUS       DAOQFI010000778         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_108989_length_1104_cov_3.093422, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000778 DAOQFI010000000
VERSION     DAOQFI010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108989_length_1104_cov_3.093422"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..>1104)
                     /locus_tag="PLE93_11395"
     CDS             complement(108..>1104)
                     /locus_tag="PLE93_11395"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HNH87698.1"
                     /translation="GPRAILLGNLFRDQLDRYGETEALADAWAGLAARRAGRTSFILN
                     SDDPLIADIGNSSGPDTIYFGIEDEAQALTELQHAFDAKLCRNCGHELTYDRAFVGHL
                     GHYHCESCGMRRPRPEISATGITLKGMSGLTATINSPEGSVELDLPLPGLYNLYNALG
                     AIATARQIGVPVSESVRSLAGMKAVFGRVERIRIGEVDLSILLVKNPAGANEVIRTLS
                     LEPAPIDLWIALNDRIADGRDVSWIWDADFEMLADRVGRVTCTGTRASEMALRLKYAG
                     WPTDRTEVIEDPEAAIDRALAQSGEQLFALPTYTALLDLRKLLADRGDTEKYWEG"
BASE COUNT          169 a          344 c          388 g          203 t
ORIGIN      
        1 tgatgcacct tccgcaagcc cgctccagac cggaaggtcg gccgtgtatc cgcaggactc
       61 gaccagctgc cagatcgcag cttcgggtga gttctcgtcg ctgaggatca accttcccag
      121 tacttctcgg tgtcgccgcg atcggccagc agttttcgca gatcgagcag cgccgtatag
      181 gtcggcaggg cgaagagctg ctctccgctt tgcgcgaggg cccggtcgat cgcggcttcg
      241 ggatcttcaa tgacctcggt gcggtcggtg ggccagccgg cgtacttgag ccggagcgcc
      301 atttccgaag ccctggttcc ggtgcatgtg accctgccta cccggtcggc gagcatctcg
      361 aagtcggcgt cccagatcca ggagacgtcg cggccgtcgg cgatccggtc gttcagcgca
      421 atccagagat cgatcggcgc gggttccagt gagagggtcc ggatcacctc gttggcgccg
      481 gccgggttct tgaccagcag aatcgacagg tcgacttccc cgatcctgat ccgctcgacc
      541 cggccgaaga ccgctttcat gccggccagg ctccggaccg attcggagac cggcactccg
      601 atctggcggg ccgtggcgat tgccccgagc gcgttataga ggttgtagag gccggggagg
      661 ggcagatcga gctcgacgct gccttcgggg gaattgatcg tggcggtgag gcccgacatg
      721 cccttcaggg tgatgccggt cgccgagatt tccggccggg gccgcctcat gccgcagctt
      781 tcgcagtggt agtggccgag atggccgacg aaggcccggt cataggtcag ctcgtggcca
      841 cagttgcggc agagcttggc gtcaaaggcg tgctggagct cggtcagggc ctgggcttca
      901 tcctcgatcc cgaaatagat cgtgtccggg ccgcttgagt tgccgatgtc ggcgatcaac
      961 gggtcgtcgg agttgaggat gaacgaggtc cggccggccc gtcgggcggc cagcccggcc
     1021 caggcgtcgg cgagcgcctc ggtctccccg tagcggtcga gttgatcgcg gaacaggttg
     1081 ccgagcagga tcgcccgggg tccg
//
LOCUS       DAOQFI010000779         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_109035_length_1104_cov_2.429933, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000779 DAOQFI010000000
VERSION     DAOQFI010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109035_length_1104_cov_2.429933"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PLE93_11400"
     CDS             <1..928
                     /locus_tag="PLE93_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87699.1"
                     /translation="SAKIDQARTRLRQRAESESGAGGLEVFEPADGKGSPDADALAAS
                     IGSLSLMPGRRYLLADRIEKWGKNQSATVAEALLAAPDETTVVLIARGKVPAGIAEAV
                     KKVGGEDLSYEAPEGARLPVHLIESAAARGFELTPEAARFMIARMGESLPRLGNELDR
                     LALWAGPDGLVDVEDLEEMVTDTSETSNFALGDAVISGDRREVIAVAERLLAQGVSAG
                     STVFSTSTSIRRAHKALVRLEAGEDPSRLERELGVPPFVARRLISSLTGTTVESMREA
                     AIAFAQLEIWTRGGAEYPDDLALDLALLAATA"
BASE COUNT          179 a          360 c          392 g          173 t
ORIGIN      
        1 cagcgccaag atcgaccagg cccgcacccg gttgcggcag cgggccgagt ccgagagcgg
       61 tgccggtggt ctggaggtct tcgaaccggc cgacggcaag ggaagccccg acgccgatgc
      121 gcttgccgcc tcgatcggtt cgctctcgct gatgccgggc cggcgatacc tgctcgccga
      181 ccggatcgag aaatggggca agaaccagtc cgcgacggtc gccgaggcgc tgctggccgc
      241 gcccgacgag acgacggtgg tacttatcgc gcggggcaag gttccggccg ggatcgcgga
      301 agccgtgaag aaggtcgggg gcgaggatct ttcctatgaa gctcccgaag gcgcccggct
      361 gccggtgcac ctgatcgaga gtgcagcggc ccggggattc gagctgaccc cggaggccgc
      421 ccgcttcatg atcgcccgga tgggggagag cctgccccgg ctcgggaacg aacttgaccg
      481 gctggcgctc tgggccggcc ccgacgggct ggtcgacgtc gaggacctcg aagagatggt
      541 caccgacaca tccgaaacca gcaacttcgc tctcggcgat gcggtaatca gcggggaccg
      601 gcgggaagtg atcgccgtcg ccgagcgcct gctcgcccag ggtgtgagtg cgggcagcac
      661 cgtcttctcg acttcgacct cgatccgccg ggctcacaag gcactggtca ggctggaagc
      721 cggggaggat ccgtcacgac tcgaacggga actcggggtg ccgccgtttg tcgccagacg
      781 gctgatcagc tcgttgaccg gaaccacggt cgaatcgatg cgtgaggcgg cgattgcctt
      841 cgcacaactg gagatctgga cccgtggcgg cgccgaatac ccggacgacc tcgccctcga
      901 cctcgccctc ctggccgcca ccgcgtgaag ctaggagccg gaggcgatac gggcagcgcg
      961 cgcttttttc ctcgcgccgt tgttgcggtg gatcgcgccc ttctgaaccg ccttgtctat
     1021 ccgcgagacc agagcgcgat gctcggcctg gacggagtcg gcgtcgcctt catcggcggc
     1081 cttctcgagg cggttgaagt aagt
//
LOCUS       DAOQFI010000780         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_109217_length_1103_cov_3.011450, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000780 DAOQFI010000000
VERSION     DAOQFI010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109217_length_1103_cov_3.011450"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..>1103
                     /locus_tag="PLE93_11405"
     CDS             40..>1103
                     /locus_tag="PLE93_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH87700.1"
                     /translation="MIGAGPSGFYAADQIIKDPETHAQVDLFDRLPTPYGLVRGGVAP
                     DHPKIKSVTRVYEKTAAKEGFRFFGNVEVGRDLNVEDFEQHYHAIIYTFGAEADRELG
                     IPGEDLPGSYAATAFVGWYNAHPDYADRNFKLDQAKRAVVIGNGNVALDVARMLALDD
                     AELRKTDIADHAIELLDASTIEEIVVLGRRGPAQAAYTNPEVKELGELEQADVIVSPE
                     EIELDPASKAWIESDEADKTARVNVEIVQEYATRQPTGKPKKVALRFLSSPVEIKGGD
                     RVESIVIGRNELVDVDGNLKARDTGEREELACDLILRSVGYTGVPLRGVPFDERRGTI
                     LNEDGRVLESHGGDHRTGHYT"
BASE COUNT          220 a          374 c          350 g          159 t
ORIGIN      
        1 agtaacccag gaacccccga aaacccgctt cgggtagcga tcatcggcgc cggaccatcc
       61 ggcttctacg ctgccgacca gatcatcaaa gatcccgaga cccacgccca ggtggatctc
      121 tttgaccgcc tgcccacccc ctacgggctg gtgcgcggcg gtgtcgcccc ggaccatccg
      181 aagatcaagt cggtgacccg ggtctacgag aagaccgctg ccaaggaggg gttccgcttc
      241 ttcggcaacg tcgaggtcgg ccgcgacctg aacgtcgagg atttcgagca gcactaccac
      301 gcgatcatct acaccttcgg ggccgaagcg gaccgtgagc tcggcatccc cggcgaggat
      361 ctgcccggca gctatgccgc cactgccttc gtcggctggt acaacgccca ccccgattac
      421 gccgaccgga acttcaagct ggaccaggcg aaacgggcgg tcgtgatcgg caacggcaac
      481 gtcgccctcg atgtggcccg catgctcgcc ctcgatgacg ccgagctgcg aaagaccgac
      541 atcgccgacc atgcgatcga gttgctcgac gcatcgacga tcgaggaaat cgttgtgctc
      601 ggccggcgcg gcccggccca ggctgcctac accaaccccg aggtcaagga gctcggggag
      661 ctcgagcagg cggacgtgat cgtaagtccg gaggagatcg agctcgaccc ggccagcaag
      721 gcgtggatcg agtcggacga ggccgacaag acagcccggg tcaacgtcga gatcgttcag
      781 gagtacgcca cgcgccagcc caccggcaag ccgaagaagg tcgcgctccg ctttctctcc
      841 tcgccggtcg aaatcaaggg tggcgacagg gtcgaaagca tcgtgatcgg ccgcaacgag
      901 ttggtcgacg tcgacggcaa tctcaaggcc cgcgacaccg gcgagcgcga ggaactggct
      961 tgtgacctga tcctgcgctc ggtcggatac accggggtcc cgcttcgcgg ggttcccttc
     1021 gacgaacggc ggggcacgat cctcaacgag gacggacggg tgctcgaaag ccacggcggg
     1081 gatcaccgca ccggccacta cac
//
LOCUS       DAOQFI010000781         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_109441_length_1102_cov_2.518625, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000781 DAOQFI010000000
VERSION     DAOQFI010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109441_length_1102_cov_2.518625"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..>1102
                     /gene="ftsZ"
                     /locus_tag="PLE93_11410"
     CDS             250..>1102
                     /gene="ftsZ"
                     /locus_tag="PLE93_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNH87701.1"
                     /translation="MPDAQDRLKRIFSEAPQDRDDGPRYGRQEPSSTPGAGVPHNPVI
                     RVIGVGGGGVNAINRMIEAQIPGVEFMAVNTDLQSLQLCMADLTVHIGGDETRGLGAG
                     ADPSLGFRAAFDEQDRIKRLLKGSDMVFIAAGAGGGTGTGAAPVIARLARDIGALTVG
                     IVTKPFTFEGSRRQKQADQGIEALAGEVDTLIVIPNDRLLEVLDQSTPMVDAFQVADD
                     VLRQGVQGISDLVTLPSVINLDFADVRSIMSDAGRALLGIGMGSGPDRALIAAEKATS
                     SPLLETPM"
BASE COUNT          185 a          379 c          371 g          167 t
ORIGIN      
        1 gccgccggcc agcaggttga aggcgagtgt agcagaggga gtcgtgggac caggaccgat
       61 gaggacggga aagacaaagc gggccgtgta gaagacgacc cgccctgcct ctccctccgc
      121 ttcggtcgcc gcgacccgct ggagggccga caggaagaac cggctcccgc caccccggcc
      181 gagcctgccc cgcgggcgga ggatccgggc ccgctttcgg cccccgaccc ggctgctgaa
      241 acttccaatg tgcccgacgc ccaggaccga ctgaagcgga ttttctccga ggctccgcag
      301 gatcgcgacg acggaccccg ctacggccgt caggaacctt cctcgactcc cggagccgga
      361 gttccacaca acccggtgat ccgggtgatc ggagtcggcg ggggtggcgt caacgcgatc
      421 aaccgcatga tcgaggccca gatcccgggt gtcgagttca tggcggtcaa caccgacctt
      481 cagtcgctcc agctctgcat ggcggacctg accgtccaca tcggtggcga cgagacccgc
      541 ggactcggtg ccggagcgga cccttcgctc ggtttccggg cagcctttga cgagcaggac
      601 cggatcaagc ggctgctcaa gggctccgac atggtcttca tcgcggccgg tgccggtggt
      661 ggcaccggaa ccggtgccgc gccggtcatt gcccggctgg cccgcgacat cggtgcgctt
      721 accgtcggga tcgtgaccaa gccgtttacc ttcgagggca gccgtcgcca gaagcaggcc
      781 gaccagggga tcgaagccct cgccggtgaa gtcgacacgc tgatcgtgat tccgaacgac
      841 cggctgctcg aggtgctcga ccagtcgacc ccgatggtcg acgccttcca ggtcgccgac
      901 gacgtgctgc ggcagggtgt tcagggcatt tccgacctgg tcacgctgcc ctcggtgatc
      961 aacctcgact tcgccgacgt gcggtcgatc atgtccgacg ccggtcgggc gctgctcgga
     1021 atcggcatgg gcagcggccc cgaccgggcg ctgatcgcgg ccgagaaggc aaccagctca
     1081 cctctgctgg aaaccccgat gg
//
LOCUS       DAOQFI010000782         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_109765_length_1100_cov_3.367464, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000782 DAOQFI010000000
VERSION     DAOQFI010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109765_length_1100_cov_3.367464"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PLE93_11415"
     CDS             <1..931
                     /locus_tag="PLE93_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87702.1"
                     /translation="LPDGVASGIQTTIGLLIAAGLAALLVWLLGKGRANPLFFAGAAT
                     LLTMWALVIVVPVEDRNFDSSRYMYPFLLASVITFGSLIRPGKPGRTLVWVAGIAVAA
                     LCLNGVVEQARHNDQQRAGVTVLLRSGLAGVEAVGPTDPSLTADDLATQEEDFFLNLP
                     FRAVDDLEIPSLAAYTAAVDQYGRLGFTGSEIGENGPGSALIADRSAYRVSGIGSPRP
                     LPEGARDLPCRRPAYLLPGPRGVAVPPGLFELRNPSGHPAALRVFRFSHAGTPAGRVP
                     ARSAALVGGPQNHGNAGLRMRSDLTLTVCHLAQ"
BASE COUNT          152 a          405 c          354 g          189 t
ORIGIN      
        1 cctgccagac ggggtcgcct ccggaataca gacgacgatc ggcctgctga tcgccgccgg
       61 tctggccgcg ctgctggtct ggctgctcgg gaagggccgg gccaatcccc tgttcttcgc
      121 gggggcggcg acgcttctga ccatgtgggc gctggtcatc gtggttccgg tcgaggatcg
      181 caacttcgac agctcccgct acatgtatcc gttcctgctg gcaagcgtga tcaccttcgg
      241 cagcctgatc aggcccggga agccgggccg caccctggtc tgggtggccg gaatcgcggt
      301 tgccgcgctc tgcctcaacg gcgtggtcga acaggctcgc cacaatgacc agcagcgtgc
      361 cggggtgacc gtcctgcttc gctccggact ggcgggggtg gaagcggtcg gccccaccga
      421 cccctcgctg accgccgatg acctcgccac ccaggaagag gacttcttcc tcaacctccc
      481 cttccgggcg gtcgacgatc tggagatccc ctcgctggcc gcctacaccg cagccgtcga
      541 tcagtacggc cggctgggtt tcaccggatc agagatcggc gagaacggcc ccggctccgc
      601 cctgatcgcc gaccggtccg cttaccgggt ctccggcatc ggatctccgc gccccctgcc
      661 cgaaggtgcc agggacctgc cttgccgacg cccggcctac cttctccccg gaccacgagg
      721 ggtcgcggtc ccgcctggcc tttttgaact ccgcaaccct tccggccacc cggccgctct
      781 ccgcgtcttc cgtttcagcc acgccggcac cccggccggc cgggtaccgg cccgttcggc
      841 agctctggtt ggcggcccgc agaaccatgg caacgccggg ttgcggatga gaagcgacct
      901 gacgctgacc gtctgccacc tggctcagta gccaacgttg atcaggctgt cggcgttggc
      961 ggcgagcgca gcgacgatca gaagcaggac cgacgggacc agaatcaggg cgatcaccag
     1021 ctggatcttc ggcgcggccc gggcggcctg ttcctcgatc gtccgccgct gatcctgcct
     1081 gagggtcgcc gactgccttc
//
LOCUS       DAOQFI010000783         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_110338_length_1097_cov_2.996161, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000783 DAOQFI010000000
VERSION     DAOQFI010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110338_length_1097_cov_2.996161"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1097)
                     /locus_tag="PLE93_11420"
     CDS             complement(<1..>1097)
                     /locus_tag="PLE93_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87703.1"
                     /translation="RSYGIYLWQSPIILLTTPALAGFSLPRAVIQVGATFLIAALSWK
                     FIEDPIRHGAIGKFMKKWRDRRIRLRKPVTPEGWGGTVAVGLIFFFALLGFAGANPKN
                     QLLPIAVTNNDPLAELGTMTVSLESEGGAGPVPSQVAGDPTKTACTSVAYVGESTSLG
                     MVESSILPNPAERLDARLKAVGATDQHIDIAGSRSVDASDPGTLSGLDAAQAIRDSGF
                     KGCWVFALAVNDSGLVAADPSGSASSRIDQMLAVADGDPVLWVNGSIRESGTLGYMKP
                     EIGDWNEALVETCQAHPEMRVYDWDEEVDPDWFEYDGVHYTPTGYSQRSRLTADALVA
                     AFPGDQPLPSGQEAGDPESCLVSTGQSTTPE"
BASE COUNT          183 a          341 c          383 g          190 t
ORIGIN      
        1 tattcgggcg tagtgctctg gccggtgctt acgaggcagc tttcggggtc gccggcctcc
       61 tggcccgagg gaaggggctg gtctccgggg aaagcggcga cgagggcgtc ggcggtgagg
      121 cggctgcgct ggctgtaacc ggtcggggtg tagtggaccc cgtcgtattc gaaccagtcc
      181 gggtccactt cttcgtccca gtcgtacacc ctcatttcag gatgggcctg acaggtctcg
      241 accagagcct cgttccagtc cccgatctcc ggcttcatgt agcccagggt tccgctttcg
      301 cggatcgaac cgttcaccca gagcaccgga tcgccgtcgg cgacggccag catctggtcg
      361 atccggctgg aagcgctgcc ggaaggatcg gcggcgacca gcccggaatc gttgactgcg
      421 agcgcgaaca cccaacagcc cttgaagccg gagtcgcgga tcgcctgggc ggcgtcgagg
      481 ccgctgagcg tgccggggtc cgaagcgtcg accgagcgtg aaccggcgat gtcgatgtgc
      541 tggtcggttg ctccgaccgc tttcaggcgg gcgtcgagcc tttcggcggg gttgggcagg
      601 atcgacgact cgaccattcc cagtgaagtg gactcgccga cataggcgac cgaagtgcag
      661 gcggtcttgg tcgggtctcc cgcaacctgg ctcgggacgg ggcccgcgcc tccttcggac
      721 tccagggaga cggtcatcgt gccgagctcg gccagcggat cgttattggt cacggcaatc
      781 ggcaggagct ggtttttcgg gttggccccg gcaaatccga gcagggcgaa gaagaagatc
      841 agccctaccg caacggtccc gccccagccc tccggggtca ccggctttcg caggcgaatc
      901 ctccggtccc gccacttctt catgaacttg ccgatcgccc cgtggcggat cggatcctcg
      961 atgaacttcc aggagagcgc ggcgatcagg aaagtggccc cgacctgaat cacggcacgg
     1021 ggcagcgaaa agccggccag ggcgggcgtg gtcagcagaa tgatcgggga ttgccagaga
     1081 tagatgccgt aggaacg
//
LOCUS       DAOQFI010000784         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_111346_length_1092_cov_2.873674, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000784 DAOQFI010000000
VERSION     DAOQFI010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111346_length_1092_cov_2.873674"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLE93_11425"
     CDS             <1..354
                     /locus_tag="PLE93_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87704.1"
                     /translation="DEEGIGQKAIAVWRGPVGYTLKMAIEAVEKVHPEFLRGVATGDV
                     VGEGTWTLATTADQWTSIEFDWNVRANRKWMEFLAPLARPLFVSSHDHVMKEGAEGLA
                     GHLSCEMRDFSASVG"
     gene            586..873
                     /gene="groES"
                     /locus_tag="PLE93_11430"
     CDS             586..873
                     /gene="groES"
                     /locus_tag="PLE93_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNH87705.1"
                     /translation="MKLKPLGDRLIVKPVDEEETTASGLVLPDTAKEKPQKGKVVAVG
                     DGAVADDGSRRPLDVAEGDEVLYSKYGGTDITVEGEELLVLRESDILAKVA"
     gene            915..>1092
                     /locus_tag="PLE93_11435"
     CDS             915..>1092
                     /locus_tag="PLE93_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNH87706.1"
                     /translation="MAHKELKFAEDARGALQAGVDAVADAVKVTLGPKGRYVVLDKKF
                     GAPTITNDGVTIARE"
BASE COUNT          226 a          327 c          346 g          193 t
ORIGIN      
        1 gacgaggaag ggatcggcca gaaggcgatc gcggtctggc gcggaccggt cggttacacc
       61 ctgaagatgg cgatcgaagc ggtcgagaag gtccacccgg agtttcttcg cggagtggcg
      121 accggcgacg tggtcgggga aggtacgtgg accctcgcga ccacggccga ccagtggacc
      181 tcgatcgaat tcgactggaa cgtacgggcc aaccgcaagt ggatggaatt cctcgccccg
      241 ctcgcacgcc ccctcttcgt ctccagccac gaccatgtga tgaaagaggg agccgaaggc
      301 ctcgccggtc atctctcatg cgagatgcgt gacttttcgg cttccgtcgg ctgagcccct
      361 ctgcttgcgg actggagccg ggtttccccg gcttgagtcg agtggactca gatttttcac
      421 atttcggggt tgaccgacgg tctcaattct gtatggttgt cagttgctgg cactctcccc
      481 ccgagagtgc caagagactg aggcgcccga atcatcgatt tcccggcgcc agaagcgatg
      541 tatggaacct gaaacacgta tgaacatgac catcggaggt aatcaatgaa gctgaagccc
      601 ctcggggacc ggcttatcgt caagccggta gatgaagagg agacgaccgc cagcggtctg
      661 gtcctcccgg acaccgccaa ggaaaagccg cagaagggca aggtcgtcgc tgtcggtgac
      721 ggcgcggtcg ccgacgacgg cagccgtcgt ccgctcgacg tcgctgaagg cgacgaggtc
      781 ctctactcca agtacggcgg aaccgacatc accgtcgagg gcgaagagct tctcgtcctg
      841 cgtgagtcgg acatcctcgc caaggtcgcc tagcgcgtcc tgacaaccag actttcagga
      901 ggtattagaa agatatggct cacaaagagc tgaagttcgc agaagatgcc aggggtgccc
      961 tgcaggccgg cgttgacgcc gtcgctgacg cagtcaaggt aaccctcggc cccaagggtc
     1021 gctacgtcgt gctcgacaag aagttcggcg ctccgaccat caccaacgac ggtgtcacga
     1081 tcgcccgcga gg
//
LOCUS       DAOQFI010000785         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_111400_length_1092_cov_2.246866, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000785 DAOQFI010000000
VERSION     DAOQFI010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111400_length_1092_cov_2.246866"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLE93_11440"
     CDS             <1..304
                     /locus_tag="PLE93_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87707.1"
                     /translation="WTFTVEERPSDPDPDPILDTHMNLSQAKFYARAQTKKRFYRAPF
                     RVSCWRKSSSSFGCFSFYRQNWRKRFRAKWVFAHVVDDNQLWYTTSFKGKSRYSGW"
     gene            complement(392..465)
                     /locus_tag="PLE93_11445"
     tRNA            complement(392..465)
                     /locus_tag="PLE93_11445"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(429..431),aa:Met,seq:cat)
     gene            complement(538..>1092)
                     /locus_tag="PLE93_11450"
     CDS             complement(538..>1092)
                     /locus_tag="PLE93_11450"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HNH87708.1"
                     /translation="KLSTQIGTGAELVFVIAHAFWGIGMILYGIFATLFANRIFFFRV
                     TPEEMNPLFWVVMGAAAITVNAGSAMIIAQSQIGYLADLEPFVEGASLVLWAWATWWI
                     PLLVIFGIWRHVVRKVPLTYSPMYWSLVFPLGMYTVATWQFATADNFSFMKEIPSYTI
                     WIALTAWLVTATGLVRSIFRRPTT"
BASE COUNT          233 a          320 c          351 g          188 t
ORIGIN      
        1 gtggaccttt actgtggagg aacggccaag cgaccccgat ccagacccga tcttggacac
       61 gcacatgaac ctctcgcaag cgaagttcta cgcgagggcg cagacgaaga agcgcttcta
      121 ccgagcaccg tttcgagtca gttgttggcg aaagtcctcc tccagcttcg gctgtttcag
      181 cttctaccgc cagaactggc ggaaaaggtt ccgtgcgaag tgggtcttcg cacacgtagt
      241 cgatgacaac cagctctggt acacaacaag cttcaaaggt aagagccgct actcgggctg
      301 gtaggcatcg ccggactaga acgttgccac tttcgttgcc accttgcgga ttttcgggcc
      361 gaatgcgaga gtgatttggc tccagctcta gtaccggggg agggactcga acccccgaca
      421 cgcggattat gattccgctg ctctaaccag ctgagctacc ccggcagggg accggtgagt
      481 ctagatgatc aggggctggc tgggcctgtc ggggacctcg ccccggggcg gcggcgatca
      541 ggtcgtgggg cggcggaaga tcgagcggac gagaccggtc gcggtgacca gccaggcggt
      601 cagggcgatc cagatcgtgt acgacgggat ctccttcatg aaggagaaat tgtcggccgt
      661 cgcgaactgc caggtggcga ccgtgtacat gcccagaggg aagaccaggc tccagtacat
      721 cgggctgtag gtgagcggca cctttctcac gacatgccgc cagatcccga agatcaccag
      781 caggggaatc caccaggtcg cccaggccca gaggaccagg gaggcgccct ccacgaacgg
      841 ctcgagatcg gccagatatc cgatctgcga ctgggcgatg atcatcgccg aaccggcgtt
      901 caccgtgatc gccgcggcac ccatcacgac ccagaagagc gggttcatct cctccggggt
      961 gacccggaag aagaagatcc ggttggcgaa cagggtggcg aagattccgt aaaggatcat
     1021 gccgattccc cagaaggcat gggcgatcac gaagacgagc tcggcgccgg tcccgatctg
     1081 ggtgctgagc tt
//
LOCUS       DAOQFI010000786         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_111651_length_1091_cov_1.849421, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000786 DAOQFI010000000
VERSION     DAOQFI010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111651_length_1091_cov_1.849421"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1091
                     /locus_tag="PLE93_11455"
     CDS             <1..>1091
                     /locus_tag="PLE93_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNH87709.1"
                     /translation="IPRSEGEEFVKRYHERFPAVTAFREQVVEEATETGFVKTLLGRR
                     RPLPELRSNQAQVRAQGVRLAMNTVVQGTAADIIKIAMIRSHQALLDEGLSSRLVLQI
                     HDELLFEGPAEEAEAVTELARREMSARCWAHRVPTRRWRSTPAPARAGSRPSSGSPDS
                     GMSDSLVVLLMVLVGVQFAAQAPVTGGLGRHTGGLVAALISFLVGNVVLLAIVAVAGQ
                     LGNFSSLGEVPLWQLAGGLIGATYVALAATTISRIGAGVIAAVTVTGQLACSVVVDHF
                     GWFGIEVHDVTGPRILGLVLLLAGAMAMLYSREEAGGGGASRAGRQTGHGIGSMASGA
                     ENRERLVICAVIFLASLAVGIQHPLNSEL"
BASE COUNT          156 a          363 c          388 g          184 t
ORIGIN      
        1 acatcccccg ctcggaaggg gaggaattcg tcaagcgcta ccacgaaagg ttcccggcgg
       61 tcaccgcctt ccgggagcag gtggtggagg aggcgaccga gaccggtttc gtcaagaccc
      121 tgctcggccg gcggcgaccg ttgccggagt tgcgttcaaa ccaggcccag gtccgggccc
      181 agggggtgcg gctggcgatg aacaccgtgg tgcagggcac cgcggccgac attatcaaga
      241 tcgcgatgat ccgcagccac caggcgcttc tcgacgaagg tctctcgagc cgcctcgtgc
      301 tgcagatcca cgacgaactg cttttcgaag gcccggcgga ggaagccgaa gcggtcaccg
      361 aactggcccg gcgggaaatg tccgcgcgct gctgggcaca ccgagtgccg acccgccgct
      421 ggaggtcgac gccggctccg gcgagagctg gctcgcggcc aagtagcggc agcccggatt
      481 ccggcatgag cgattccctg gtcgtcctgc tgatggtcct ggtcggggtc cagtttgccg
      541 cccaggcccc ggtcaccggc gggctcggcc gccacactgg cgggctggtc gcggctctga
      601 tctccttcct ggtcggcaac gtggtgctgc tggccatcgt cgcggtcgcc ggccagctcg
      661 gcaacttctc ttccctcggt gaggtaccgc tctggcagct ggccggcggg ctcatcggtg
      721 ccacctatgt ggccctcgcg gcgaccacga tctcgcggat cggtgccggc gtaatcgccg
      781 cggtcacggt taccggtcaa ctcgcctgtt cggtggtggt cgatcacttc gggtggttcg
      841 ggatcgaagt ccacgacgtg accgggccgc gaatcctcgg tctcgtgctg ctcctggccg
      901 gggcaatggc gatgctctac agccgggagg aggcaggcgg cggaggggct tcccgggccg
      961 gccggcagac gggtcacggg atcgggtcga tggccagcgg cgccgagaac cgcgagcgtc
     1021 tggtcatctg cgcggtgatc ttcctcgcct ccctggcggt cggaatccag catccgctca
     1081 actccgagct g
//
LOCUS       DAOQFI010000787         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_112760_length_1085_cov_3.463107, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000787 DAOQFI010000000
VERSION     DAOQFI010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112760_length_1085_cov_3.463107"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLE93_11460"
     CDS             complement(<1..359)
                     /locus_tag="PLE93_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH87710.1"
                     /translation="MPTSMERRHNRRRRHERTRQELIEAALNQSAAGSFKDLTVEGVT
                     REAGVARSAFYVYFGDKEELLLGALEDLIASHQNRLGRCWQQGDDPRRDIERGIYGMA
                     RIYAGNSALLALAGEAAT"
     gene            complement(411..>1085)
                     /locus_tag="PLE93_11465"
     CDS             complement(411..>1085)
                     /locus_tag="PLE93_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87711.1"
                     /translation="PDCSDSCVFRNHLALAKIDPGTGDLVESFGSGGERLIPPNECSR
                     GQAGKAGDWNICRVRPPGINGSVRFAGAGSKHPSLVLKASLGDPPEGLVGTRQRLTVD
                     LPRKLRLRPGGLKGRVRVSVSPATPRTVKVQGKRIAILAKPEYNPYETAGESDAKDSR
                     LTFRITVNRGALAPITRPGRLHKRVFGLRGEFNPVWNGFPTPEGGPWFSPNSAFRRIK
                     AAAPSS"
BASE COUNT          174 a          372 c          354 g          185 t
ORIGIN      
        1 gtggccgcct cgccggccag ggcaagcagg gccgagttgc cggcgtagat ccgggccatt
       61 ccgtagatgc cccgttcgat gtcgcggcgg gggtcgtcac cctgctgcca gcagcggccg
      121 agccggttct ggtgggaggc gatcagatcc tccagcgccc ccagcagcag ctcttccttg
      181 tcgccgaaat agacgtagaa cgccgagcgg gcgaccccgg cctcgcgggt taccccttcg
      241 acggtcaggt ccttgaacga accggccgcc gactggttca gggcagcctc gatcagctcc
      301 tgccgggtcc gctcatgacg gcgtcgtcgg ttatgtcggc gctccatcga ggtcggcacc
      361 cgccaagcat atgcaccacc cccgccggaa acccgaccgg cacgaatcgc tcaggaagag
      421 ggcgcggccg ccttgatccg gcggaaggcg gaattggggc tgaaccaggg cccgccttcc
      481 ggggtcggga acccgttcca gaccggattg aactcgccgc gcaacccgaa gacccgcttg
      541 tggagccggc ccggcctggt gatcggggcc agagcgccgc ggttgaccgt gatcctgaaa
      601 gtcagcctcg aatcctttgc gtctgactca cccgcagtct cgtaggggtt gtattcgggc
      661 ttggccagga tcgctatccg tttgccctgg accttgaccg tgcgcggggt ggcgggactt
      721 accgatacgc gaacgcgacc cttcagcccg cccggacgca gccgcagctt ccttggcaga
      781 tcgacggtga gcctttgccg ggtaccgacc aatccttccg gcgggtcgcc gaggcttgcc
      841 ttgagtacca gcgacggatg tttacttccg gctccggcga acctgaccga accgttgatc
      901 ccgggcggtc gcacccgaca gatgttccag tcacccgcct tgccggcctg cccccggctg
      961 cattcgttcg gcggaatcag gcgttcaccg ccggaaccga agctctcgac gagatcgccg
     1021 gtccccgggt cgatcttggc cagggccagg tgatttcgaa agacacagga atcggagcag
     1081 tccgg
//
LOCUS       DAOQFI010000788         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_112769_length_1085_cov_3.219417, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000788 DAOQFI010000000
VERSION     DAOQFI010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112769_length_1085_cov_3.219417"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..663)
                     /locus_tag="PLE93_11470"
     CDS             complement(34..663)
                     /locus_tag="PLE93_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87712.1"
                     /translation="MPFFARHRPRRGFLPALFASLALSLLLAGCGEDRSNLIPEETSN
                     SLISKINRAEALAADHKCFAAGDVAASAQAEIEGLGGKIDPELKRSLIDGVTSLQTML
                     SDQDICIDEGTVTEEPIQTEVPETDTGTGGTTGETGTTDTQTTTTGTQGTTTDENQNT
                     DQTQNGDNGQQTEPTPPVTNPTSPTTPTNPTTPTTPPSTGPGSGGLGPG"
     gene            complement(718..>1085)
                     /locus_tag="PLE93_11475"
     CDS             complement(718..>1085)
                     /locus_tag="PLE93_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNH87713.1"
                     /translation="EYVVDSDLAEDNLDFQEQRVALIGHSHIALYFTRVDEMSRVSSV
                     LAPEGTELEMSAGKWLLNPGSAGQPRDGDPRSAWAELDTESFQARFHRVQYPVEEAAE
                     AIRKAGLPSHLGDRLFKGQ"
BASE COUNT          155 a          321 c          375 g          234 t
ORIGIN      
        1 gtcagcgaga atcgtcggtt cggtcatcgt ggattacccg gggccgagcc cgccagagcc
       61 agggcctgtg ctcggtggtg tggtcggggt cgtggggttg gtcggggtcg tcggactggt
      121 cggattggtg accggcgggg tgggctcggt ctgctggccg ttgtcgccgt tctgggtctg
      181 atccgtgttc tggttttcat cggtggtggt gccctgggtg ccggtggtgg tcgtttgggt
      241 gtccgtggtg ccggtctctc cggtcgtgcc tccggtcccg gtgtcggttt ccggcacttc
      301 ggtctggatc ggctcttccg taacggttcc ctcgtcgatg cagatgtcct gatcactgag
      361 catggtctgg agggatgtca caccgtcgat cagtgaacgt ttcagctccg ggtcgatttt
      421 gccgccgagt ccttcgatct cggcctgggc cgaagccgcc acgtcaccgg ccgcaaaaca
      481 cttgtgatcg gcggccagcg cctcggcacg gttgatcttg gagatcagcg aattcgacgt
      541 ttcctcaggt atcaggttac tccgatcctc gccacatccg gccaggagca gggagagggc
      601 caggctggca aacagggcag gcagaaaacc ccggcggggg cggtgtcggg caaaaaacgg
      661 cattcccgcc gagtgtagat ggttggaccg ctggcctttg tgacgcatcc cggaccttta
      721 ctggccctta aagagccggt cacccagatg ggagggaaga ccggcctttc ggatcgcttc
      781 tgcggcctct tcgaccgggt actgaacccg gtggaaacgg gcctggaagc tctccgtgtc
      841 cagttccgcc caggctgacc gtgggtcgcc gtcccgcggc tgcccggcgc tgcccgggtt
      901 gagcagccac ttcccggcgc tcatctcgag ctcggtgcct tccggcgcca gcacggacga
      961 aaccctggac atctcgtcaa cccgggtgaa gtagagggcg atgtgggaat ggccgatcag
     1021 ggcgacccgc tgttcctgga agtcgagatt gtcttcggcc agatcgctgt caacgacgta
     1081 ttccc
//
LOCUS       DAOQFI010000789         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_112917_length_1085_cov_1.577670, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000789 DAOQFI010000000
VERSION     DAOQFI010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112917_length_1085_cov_1.577670"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLE93_11480"
     CDS             complement(<1..388)
                     /locus_tag="PLE93_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNH87714.1"
                     /translation="MSADKRVLILGSTGSIGTQALEIIAGREDLEVAGLAVGRDWQTA
                     VAQAKGFSVGTIAVADQAAAAEASAGFDGNVLAGEPGVIELIGATAPDIVLNGIVGAA
                     GLGPTIAALTQGIDVALANKESLVIGG"
     gene            complement(436..>1085)
                     /locus_tag="PLE93_11485"
     CDS             complement(436..>1085)
                     /locus_tag="PLE93_11485"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNH87715.1"
                     /translation="ESKGVRETDECVAGLLGASWDALAATVFAGQGDADRFTGQYGAA
                     RRKDRTGVTGSVAATLLGIYWIGLALAHAVLLRELPDHGGALVVDVLVGTFACDTGAY
                     AVGRMFGSHKFAPNLSPNKTIEGLIGGFLIGMLGVWCAGLYQDWLSGVDALILGAAVA
                     AVAPVGDLFESMIKRDLGTKDTGNLFGPHGGLLDRLDAVFFTIVVGYYVSVALVY"
BASE COUNT          191 a          385 c          347 g          162 t
ORIGIN      
        1 ccccgccgat gaccaggctt tccttgttgg cgagggcgac atcgatgccc tgtgtcaggg
       61 ctgcgatggt cgggccgagt ccggccgccc cgacgatccc gttcagcacg atgtcgggag
      121 cggtggcgcc gatcagctcg atcactcccg gctcgccggc cagcacgttc ccgtcgaaac
      181 cggccgaggc ttcggcggca gcggcctgat cggcgaccgc gatggtcccg accgagaagc
      241 ccttggcctg ggcgaccgcc gtctgccagt cccggccgac cgccaggcct gccacttcca
      301 ggtcttcccg gccggcgatg atctcgaggg cctgggtgcc gatcgagccg gtcgaaccga
      361 ggatcaggac ccgcttgtca gcactcattt tcctgattcc ggaagtccgg gaattgagga
      421 atcagcgcta atggcctagt acaccagcgc tacggagacg tagtagccga ccacgatcgt
      481 gaagaagacc gcgtcgaggc ggtcgagcag accgccgtgc gggccgaaca ggttgccggt
      541 gtccttggtc cccagatccc gcttgatcat cgactcgaag aggtcaccca ccggggcgac
      601 ggcggccact gccgcaccca gaatcagtgc gtccactccg gaaagccagt cctggtagag
      661 accggcgcac cagaccccga gcatgccgat caggaatccg ccgatcagcc cttcgatcgt
      721 cttgttcggt gaaaggttcg gcgcgaactt gtggctgccg aacatccggc cgaccgcgta
      781 ggccccggtg tcacaggcga aggtgccgac caaaacgtcg accacgaggg cgccaccgtg
      841 atcaggaagt tcgcgcagca gtaccgcatg agcgagggcc agaccgatcc agtagatccc
      901 gagcaaggta gccgcgaccg agccggtcac ccccgtccgg tccttgcggc gggccgcccc
      961 gtactggccg gtgaaccggt ccgcgtcgcc ttggccggcg aacaccgtcg ccgccaacgc
     1021 atcccacgac gcccccaaca ggccggcgac gcactcgtcg gtctcgcgga cacccttcga
     1081 ctcgg
//
LOCUS       DAOQFI010000790         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_113196_length_1083_cov_3.156615, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000790 DAOQFI010000000
VERSION     DAOQFI010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113196_length_1083_cov_3.156615"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..>1083)
                     /locus_tag="PLE93_11490"
     CDS             complement(130..>1083)
                     /locus_tag="PLE93_11490"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNH87716.1"
                     /translation="GRSSQDLAAKIAGKLSLAVGDVTLKTFSDGEIYGRYEESIRGAD
                     VFLVQSTCANVETDMTPNDALMELMIMADAAQGASAHRIIAVMPWFGYARQDKKSAPR
                     EPITSRVVARSLEAVGIDRVLTMDLHAGQIQGFFTVPVDHMTAQPMLTQWFADQYDPS
                     ELVIVSPDAGRAKAARNFARKVGTEWAIMEKERPKQQVAEIGYVVGDVKDKIAIISDD
                     MIDTAGTLCAAARTVLDEGAKSVIACATHGVFSGPAFERLPYEKSGLEKIVVTDTIPL
                     RPGAPDNIHVLSTAGTLADSIRRIFTDDSVSEIFAGENQLF"
BASE COUNT          175 a          324 c          384 g          200 t
ORIGIN      
        1 accgcttcac cgacctcgtc ctccgcggca tacgtgtgta gcgatcagtt ctcaaagatc
       61 gagatggcga aagtgagcgc tatatggcca cttttgcctt ctcgattcct gcgcgactgc
      121 tgctaagccc taaaacagct ggttttcgcc ggcgaagatc tcgcttaccg agtcgtcggt
      181 gaagatgcgg cggatcgagt cggcgagggt tccggcggtg ctgaggacgt ggatgttgtc
      241 cggggcgccc gggcggagcg ggatcgtgtc ggtgacgacg atcttctcga ggcccgactt
      301 ctcgtagggc agccgctcga aggcggggcc ggagaagacg ccatgggtgg cgcaggcgat
      361 caccgacttg gcgccctcgt cgaggacggt gcgggcggcg gcgcagagcg tgccggcggt
      421 gtcgatcatg tcgtcggaga tgatcgcgat cttgtccttc acgtccccga ccacgtagcc
      481 gatctcggcg acctgctgct tgggccgctc cttctccatg atcgcccact cggtcccgac
      541 cttgcgggcg aagttgcggg cggccttggc gcgaccggcg tccggggaga cgatcaccag
      601 ctccgaaggg tcgtactggt cggcgaacca ctgggtcagc atcggctggg cggtcatgtg
      661 gtcgaccggg accgtgaaga agccctggat ctggccggcg tgaaggtcca tggtcaggac
      721 ccggtcgata ccgacggctt cgagtgaacg agcaaccacg cgggaggtaa tcggctcgcg
      781 gggggccgac ttcttgtcct ggcgggcgta cccgaaccag ggcatgacgg cgatgatccg
      841 gtgggcggag gcgccctggg cggcgtcggc catgatcatc agttccatca gggcgtcgtt
      901 cggcgtcatg tccgtctcga cgttggcgca ggtcgactgg acaaggaaga cgtcagcgcc
      961 gcgaatcgat tcctcgtagc ggccgtagat ctcgccgtcg gaaaaggtct tcagggtgac
     1021 gtcaccgacc gcaagcgaaa gcttgccggc gatcttcgcg gccaggtcct gggagctccg
     1081 gcc
//
LOCUS       DAOQFI010000791         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_113382_length_1082_cov_3.986368, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000791 DAOQFI010000000
VERSION     DAOQFI010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113382_length_1082_cov_3.986368"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..600
                     /locus_tag="PLE93_11495"
     CDS             94..600
                     /locus_tag="PLE93_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNH87717.1"
                     /translation="MAFQPSPGEQIIFQGHPSWRSILAFYLKGLLIGLIVAVIAKLLG
                     EGFATVFIIVLIALGLSVLIGFIKRMATVYTITNRRLNIKRGIFARDIQETRLERVQN
                     VSYNQTVFQRMLRVGDVDFDTAATDQDNLFCFHGVADPQEVVTEVDKATSLGASGSHG
                     LGEPSGQY"
     gene            690..>1082
                     /locus_tag="PLE93_11500"
     CDS             690..>1082
                     /locus_tag="PLE93_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87718.1"
                     /translation="MTDSLASQSPELLAINTIRTLSIDAIDKANSGHPGTAMALAPLA
                     YKIWQDYLRYDPADPHWPNRDRFVLSAGHASMLIYSVLHLAGVKKVEHDGTISDRLSV
                     TLEDIENFRQLGSSTPGHPESHLTTGVET"
BASE COUNT          193 a          379 c          306 g          204 t
ORIGIN      
        1 ggacaccggt cgaggttacg cgggcgaacg gacgagcgac gccagaccgg ccggctttca
       61 cacatatccg ctccggtcga atatgctcgc gccgtggctt ttcaacctag tcccggcgag
      121 cagattattt tccagggcca cccgtcctgg cgttcgattc tcgccttcta cctgaagggc
      181 cttttgatcg gcctgatcgt cgcggtcatc gcgaaattgc tcggcgaggg tttcgcgacg
      241 gtcttcatca tcgtcctgat cgcgctcgga ctttccgtgc tgatcggttt catcaagcgg
      301 atggcgacgg tctacacgat caccaaccgc cggttgaaca tcaagcgggg catcttcgcc
      361 cgcgacatcc aggagacccg gctcgagcgg gtccagaacg tcagctacaa ccagaccgtg
      421 ttccagcgga tgctgagggt cggcgacgtc gacttcgaca ccgccgcaac cgaccaggac
      481 aaccttttct gctttcacgg ggtcgccgat ccgcaggaag tggtgaccga ggtggacaaa
      541 gcgacctcgc tcggcgccag cggttcgcac gggctcggcg agcccagcgg ccagtactga
      601 gcgcagcgcc gacgcgcccc cggcgcattc acgcaaaatc cgcatttcct gttctctggc
      661 ctgaagcggt tttccgtagg attgcgggcg tgaccgactc cctcgcttcc cagtctcccg
      721 agctcctggc aatcaacacc atccggacgc tttcgatcga tgcgatcgac aaggccaact
      781 caggccaccc cggcaccgcg atggccctgg ccccgctggc ctacaagatc tggcaggact
      841 acctccgcta cgacccggcc gatccgcact ggccgaaccg tgaccgcttc gtactttcgg
      901 ctggtcacgc ctcgatgctg atctactcgg tcctgcacct cgccggagtc aagaaggtcg
      961 agcacgacgg cacgatttcc gatcgtctct cggtcaccct cgaggacatc gagaacttcc
     1021 gccagctcgg ctcttccacc cccggtcacc cggaatcgca cctgaccacc ggcgtcgaga
     1081 cg
//
LOCUS       DAOQFI010000792         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_114086_length_1079_cov_2.543945, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000792 DAOQFI010000000
VERSION     DAOQFI010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114086_length_1079_cov_2.543945"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1079)
                     /locus_tag="PLE93_11505"
     CDS             complement(<1..>1079)
                     /locus_tag="PLE93_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNH87719.1"
                     /translation="SEIPLDDPVTYEMLARGDSVGVFQLESDGMREAMRRVVPTEFDD
                     ITALVSLYRPGAMKEIPRYAEGKHDPESVEYSDPRLKEITESTYGCFLYQEQLMAVAK
                     QMAGFEPSEADDLRKAIGKKKREMMAAMKPKFMEGLKASGTDHALATYLWSINEAAAD
                     YSFNKSHAACYALISYRTAYLKANYPAEYMAAVISSVMSTKDKVPFFVNHCSSMGIRV
                     LPPDVNISDHSFVVDEGNIRFGLDAVKNVGYSAVDAIINARNDRPFESLWDFCERVDN
                     RTVNKRAVECLIKCGAFDLLPGSRQGMLEKLPDAAAAGAKAQSDLMLGQESIFSLGGA
                     DEGAGGGSHEPISEVEFDQKELLAL"
BASE COUNT          185 a          345 c          335 g          214 t
ORIGIN      
        1 ccagggcgag cagctccttc tggtcgaact cgacctcgct gatcggctcg tgactgcctc
       61 cgcctgctcc ttcgtcggcc cctcccagac tgaaaatcga ttcctgcccc agcatcaggt
      121 cggactgggc cttggcgccg gccgccgccg cgtcgggcag cttctccagc atgccctggc
      181 gggagccggg cagcaggtcg aaggcgccgc acttgatcag gcattcgacc gcccgtttgt
      241 tgaccgtgcg gttgtcgacc cgttcgcaga aatcccaaag cgattcgaag ggccggtcat
      301 tgcgggcatt gatgatcgcg tcaaccgccg agtagccgac attcttgacc gcgtcgaggc
      361 cgaaacgaat gttgccctcg tcgaccacga agctgtggtc ggagatgttc acgtccggcg
      421 gcagcacccg gatgcccatc gaggagcagt ggttgacgaa gaacggcacc ttgtccttgg
      481 tgctcatcac cgaggagatc acggcggcca tgtactcggc cgggtagttc gccttcaggt
      541 acgcggtgcg gtaggagatc agcgcgtaac aggcagcatg ggacttgttg aaggagtagt
      601 cggcggcggc ttcgttgatg ctccagaggt aggtggcgag ggcatggtcg gtgccggaag
      661 ccttcagccc ctccatgaac ttcggcttca tcgcggccat catctcgcgc ttcttcttgc
      721 cgattgcctt gcgcaggtca tccgcttcgg atggttcgaa gccggccatc tgcttggcga
      781 ccgccatcag ctgctcctgg tagaggaagc agccataggt cgattcggtg atctccttca
      841 gccgggggtc tgaatactcg accgattccg gatcatgctt gccctccgcg tacctgggga
      901 tctccttcat cgcgccgggg cggtagagcg acaccagcgc ggtgatgtca tcgaactcgg
      961 tcggaacaac ccgccgcatc gcctcccgca tgccgtccga ctcaagctga aacaccccga
     1021 ccgaatcgcc gcgggccagc atctcgtaag tgaccggatc atcgagcggg atctcgctg
//
LOCUS       DAOQFI010000793         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_114443_length_1077_cov_2.709393, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000793 DAOQFI010000000
VERSION     DAOQFI010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114443_length_1077_cov_2.709393"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLE93_11510"
     CDS             complement(<1..329)
                     /locus_tag="PLE93_11510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH87720.1"
                     /translation="MNLRLLTTLAAAQFLMVLDQAVMNVSISQLVEDFDTTVTTIQAI
                     IAFYALVMAGLMLIGGKLGDIYGRKRIFKIGLVIYACGSALTAASWSVASLALGWSVL
                     EGIGAAMV"
     gene            complement(363..>1077)
                     /locus_tag="PLE93_11515"
     CDS             complement(363..>1077)
                     /locus_tag="PLE93_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNH87721.1"
                     /translation="TIEPATRDFGTRLTDKGPGTPQTFTLTSTGTTDLELGAAALTGA
                     ASTQFEITSDACSNQTLPPSASCEIGVAFGPTTAGEKVAELSVPIAGVGPATAQLTGS
                     GQKPVPPPDPCAPVAIRKVAYYKPSIKKRSNIPGVRARITTAGPAVVRISSKLIYHLN
                     GRQGQISYKQRQFAVAGHSVNYKVAIPGRLRARLKPKKQVRFVITYASKARNPQCTKF
                     ARKKTRNLTTRIVWVIPNA"
BASE COUNT          180 a          329 c          371 g          197 t
ORIGIN      
        1 accatcgcgg caccgatccc ctcgaggacg gaccagccca gggcgaggct ggcgacgctc
       61 caggaagcgg cggtaagcgc cgagccgcag gcgtagatga cgaggccgat cttgaagatc
      121 cgcttgcgcc cgtagatgtc accgagcttg ccgccgatca gcatcaggcc ggccatgacc
      181 agcgcgtaga aagcgatgat cgcctggatc gtggtgaccg tggtgtcgaa gtcctcgacc
      241 agctgggaga tcgagacgtt catcaccgcc tggtcgagca ccatcaggaa ctgggcagcg
      301 gccagcgtcg tcagcaatcg caggttcatg gcgtgattat ccgtcagcca gccgacggga
      361 catcatgcgt tcgggatgac ccagacgatc cgcgtggtca ggttccgggt cttcttccgg
      421 gcgaacttcg tgcattgggg attgcgagcc ttgctggcgt aggtgatcac gaagcggacc
      481 tgcttcttcg gcttgagcct ggctcgcagc ctgcccggaa tcgcgacctt gtagttgacc
      541 gaatgcccgg cgacggcaaa ctgccgctgc ttgtaactga tctggccctg cctgccgttc
      601 aggtggtaga tcagcttcga ggaaatcctg accacggccg ggccggcggt ggtgatccgc
      661 gccctcaccc cgggaatgtt cgagcgcttc ttgattgatg gcttgtagta ggcgaccttc
      721 ctgatcgcga ccggcgcgca gggatcgggc ggagggaccg gcttctgccc ggaaccggtc
      781 aactgcgccg tggccgggcc gaccccggcg atcggaaccg agagttcggc caccttttcg
      841 cccgccgtcg tcggtccgaa agccaccccg atctcacagc tcgccgacgg gggcagggtc
      901 tggttcgagc aggcgtccga agtgatctcg aactgggtgg acgcggcgcc cgtaagtgcc
      961 gcggcaccaa gctcgaggtc ggtggttccg gtgctggtca gggtgaaggt ctgcggagtc
     1021 ccgggacctt tgtcggtcag gcgggtgccg aaatccctgg tggcaggttc gatggtg
//
LOCUS       DAOQFI010000794         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_114844_length_1075_cov_3.059804, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000794 DAOQFI010000000
VERSION     DAOQFI010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114844_length_1075_cov_3.059804"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLE93_11520"
     CDS             <1..1011
                     /locus_tag="PLE93_11520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH87722.1"
                     /translation="WRMTRPLRSISDAARRLRGRETEIPWAEVMVEEKVEPDWTSWLD
                     TFFGEELERIDAALAETPPEEAWQLFRGLGDDLWAVLLTREYDLYPNIKALLPGAPEP
                     GLQWMWNGATGLELLGQSKNFYARVRELNERHGRAPLDQARILDFGCGWGRLTRFFAR
                     DAMPDRLFGCDPTPEILEVCAETGVPGELRQTGFLPERLPFENLDLAFSFSVFTHISE
                     AAHEACLKAIHAALAPGGLAVITVRPPAYLDHDPLMEAARAELSDDFASREMKEPRFI
                     FVPHPVEDGHPQYDEGEMTYGDTVISLPYIEENWTGLFEIVETGFLTGDMYQVPIALK
                     KR"
BASE COUNT          185 a          370 c          347 g          173 t
ORIGIN      
        1 tggcggatga cccgtccgct ccggtcgatc agcgatgctg cccggcgctt gcgcggccgc
       61 gaaacggaga ttccgtgggc cgaggtgatg gtcgaggaga aggtcgaacc ggactggacc
      121 tcctggctcg acaccttctt cggcgaggag ctggaaagga tcgacgctgc cttagccgag
      181 accccgccgg aggaggcgtg gcaactgttc cgcggactcg gcgatgacct ctgggccgtc
      241 ctgctgaccc gcgagtacga cctctacccg aacatcaaag cgctcctgcc cggcgccccg
      301 gaacccggcc ttcagtggat gtggaacggc gcgaccggcc tcgaactgct gggccagagc
      361 aagaacttct acgccagggt ccgagagctg aacgagcggc acggccgggc cccgctcgac
      421 caggcccgga tcctcgactt cggctgtggt tggggccggc tgacccgctt cttcgcccgg
      481 gacgcaatgc cggaccggct cttcggctgc gaccccaccc ccgagatcct cgaagtctgc
      541 gccgagaccg gcgtgcccgg cgaactccgg cagaccggtt tcctgcccga gcggcttccg
      601 ttcgagaacc tcgacctggc cttcagcttc tcggtcttca cccacatttc cgaagcggct
      661 cacgaggcct gcctcaaggc gatccacgcc gccctggcgc ccggtggcct cgccgtgatc
      721 acggtgcgtc cgccggccta cctggaccac gacccgctaa tggaagcggc ccgggcggag
      781 ctttcggatg atttcgccag ccgggaaatg aaggagccga ggttcatctt cgtgccccac
      841 ccggtcgagg atggtcaccc tcaatacgac gagggcgaga tgacctacgg cgacacggtg
      901 atcagcctcc cctacatcga agagaactgg accggccttt tcgagatcgt cgaaaccgga
      961 ttcctcaccg gcgacatgta ccaggtcccg atcgccctga agaagaggta ggccggggcg
     1021 aagctcgccg acctagagtt cttcggcgat gcggggggct tcgcggctga agacc
//
LOCUS       DAOQFI010000795         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_115107_length_1074_cov_2.643768, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000795 DAOQFI010000000
VERSION     DAOQFI010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115107_length_1074_cov_2.643768"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1074
                     /locus_tag="PLE93_11525"
     CDS             <1..>1074
                     /locus_tag="PLE93_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87723.1"
                     /translation="YDPDHGNELWRFNGNSAELVKDIWPGPEDSTPREFVRVGNRIFF
                     VAHEPTSGRELWQIDAGGATLVADINSGTGTSNPSYLTAYLDKVYFSAYTAAHGYELW
                     SSDGTTTAEVGDLRPGSASSDPGSLTVAGGTLFMVANDGTNGYELFKSSGGPATLVAD
                     IQPGGNSSSPTSMLPFNNKLIFTANTSVSGSEPWITDGVSVTQIGDLAPGIDSSYASY
                     YSRMNGKVYFVAYLSDPRLYETDGVTASPTTATFPEDASGFYNLAVVGNRLYLSIGTP
                     KTGLEPWVFDGAGARMIRDILPGPDSSYPTAYAKRGNGVIFAADDGTHGAEPWLLHPD
                     REVKLRPGSSRLGVSKQGFAGLKL"
BASE COUNT          202 a          379 c          309 g          184 t
ORIGIN      
        1 ctacgatccc gatcacggca acgagctctg gcgcttcaac gggaattccg cggagctggt
       61 caaggacatc tggccgggcc ccgaagattc gaccccccgg gaattcgtgc gggtcggaaa
      121 ccgcatcttc tttgtggccc acgagccgac cagcggccgc gagctctggc agatcgatgc
      181 cggcggagca accctggtcg ctgacatcaa ttcgggaacc ggcacttcga accccagtta
      241 cctgaccgcg tacctggaca aggtttactt ctcggcctac accgccgccc acggatacga
      301 actgtggtcg agcgacggaa ccaccacggc cgaagtcggt gacctccgcc ccggttccgc
      361 ttcctcggac ccgggttccc tgacagttgc cggcggaacg ctgttcatgg tcgcaaacga
      421 cggcaccaac ggctacgaac tcttcaagag ctccggcggg ccggccaccc tggtcgccga
      481 catccagccc ggcggcaact catcttcacc aacctcgatg ctgcccttca acaacaaact
      541 catattcacg gccaacacga gtgtgtccgg ctccgaaccc tggatcaccg atggcgtcag
      601 cgtcacccag atcggcgacc ttgccccggg gatcgattct tcctacgcca gctactacag
      661 ccggatgaac ggcaaggtct actttgtcgc ctacctttcc gatccccggc tgtacgagac
      721 cgacggtgtc acggccagtc ccacgaccgc caccttcccg gaagacgcct ccggctttta
      781 caacctggcc gtggtcggca accggctcta cctgtcgatc ggcactccga agaccgggtt
      841 ggaaccctgg gttttcgacg gagccggcgc ccgcatgatc agggacatcc tgcccggtcc
      901 ggattcctcg tatccgaccg cgtacgccaa acggggcaac ggggtgattt tcgcggccga
      961 tgacggcacc cacggggccg agccctggct gctccacccc gaccgggagg tgaagctcag
     1021 gccgggaagt tcaaggctgg gcgtctcgaa gcagggcttc gccggactga agct
//
LOCUS       DAOQFI010000796         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_116380_length_1068_cov_2.961500, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000796 DAOQFI010000000
VERSION     DAOQFI010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116380_length_1068_cov_2.961500"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLE93_11530"
     CDS             <1..454
                     /locus_tag="PLE93_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87724.1"
                     /translation="ASEGVSMANTGDARIVSDDELTGLPEEVGHSLFWAGPKSGTQVE
                     FSSDGTGNVHLRYLTDGAEAGDPNQSYLNIGSYPFADAYEATRKLANGPDVVKVTEGE
                     GTGFYDPNNPYSVIIAWPSEPDLQVEVYDPVKNRALEVVRSGDIVPVS"
     gene            523..>1068
                     /locus_tag="PLE93_11535"
     CDS             523..>1068
                     /locus_tag="PLE93_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87725.1"
                     /translation="MVRGRVLPWTLLPATGFAAIIAVGGIITCFPGLAEFTTGAVTGL
                     AVAGYLALFTGGPRGPARSPAFFKPRSEWVWPAVAAAGVFFVFGAPVILSGSATFAGY
                     VKLDDTSTWLAFTDHVMAHGHSVAGLAPSSYEATVQINLSSGYPLGAFIPLGVGHELT
                     GQDSAWLFQPYLALMASFMSLV"
BASE COUNT          158 a          364 c          330 g          216 t
ORIGIN      
        1 ggcctcggag ggcgtttcta tggccaacac cggtgatgcc cggatcgtca gcgatgacga
       61 actcaccggc ctgccggaag aggtgggtca cagtttgttc tgggcgggac cgaagtcggg
      121 cacccaggtc gagttcagct ccgacggaac cggcaacgtc cacctccgtt acctgaccga
      181 cggggccgaa gccggtgacc cgaaccagag ctacctcaac atcggcagct accccttcgc
      241 cgacgcttat gaggcgaccc ggaagctcgc caacggaccg gatgtggtca aggtcaccga
      301 aggtgaagga accggatttt acgacccgaa caatccttac agcgtgatca tcgcctggcc
      361 gagcgagccc gacctccagg tcgaggtgta tgacccggtc aagaaccggg ccctcgaagt
      421 cgtccgcagt ggcgatatcg tgcctgtgtc ctgattctcg cctgggtcat cttccctctc
      481 gtcctgctgg cgctaagcgt cggtgtcggt ctgctggccg aagtggtccg cggccgggtt
      541 ctgccctgga cgctcctgcc ggcgaccggt ttcgcggcca tcatcgcggt cggcgggatc
      601 atcacctgtt tcccgggcct ggccgagttc accaccggag ccgttaccgg gctggcggtg
      661 gccggttacc tggcgctctt caccggtggt ccccgcggac cggcgcgcag tccggccttc
      721 ttcaagcccc ggtccgaatg ggtctggcct gccgttgccg cggccggggt cttcttcgtt
      781 ttcggggccc cggtcatcct ctcgggatcg gccacgtttg ctggttacgt caagctggac
      841 gacacctcga cctggctcgc cttcaccgac cacgtgatgg ctcacggaca ttcggttgcc
      901 ggcttggccc cgtccagtta cgaagcgacc gttcagatca atctctcatc cggctatccg
      961 ctcggcgcgt tcattcccct gggggtcggt cacgagttga ccggccagga ctcggcctgg
     1021 ctcttccagc cctatctggc cctgatggcc agcttcatgt ccctcgtc
//
LOCUS       DAOQFI010000797         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_117270_length_1064_cov_2.815659, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000797 DAOQFI010000000
VERSION     DAOQFI010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117270_length_1064_cov_2.815659"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLE93_11540"
     CDS             <1..298
                     /locus_tag="PLE93_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNH87726.1"
                     /translation="EISAAARKNPLSAAGGLLLKPALGGLRHDLHPDTTGGAILLGLR
                     KTAVVGHGSSGSDGVANAIRLAARSARVDAPGLTEEMLHRVGVNRGSIDSATDV"
     gene            311..556
                     /gene="acpP"
                     /locus_tag="PLE93_11545"
     CDS             311..556
                     /gene="acpP"
                     /locus_tag="PLE93_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNH87727.1"
                     /translation="MNRDEVMDLVRNHLVTELELGPEQITETARFKEDFDADSLDLYE
                     LVMELEDQYGITVSEQEAAEIKTVGDALDFVVAKAVA"
     gene            531..>1064
                     /locus_tag="PLE93_11550"
     CDS             531..>1064
                     /locus_tag="PLE93_11550"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HNH87728.1"
                     /translation="MSWLKRSRERAGGHDPVTPDSGLASLIEGLPEDLKVPALTHSSW
                     TSERTDSYERLALLGDSVLGLAVADELYRTLPGLSAGALTKILNQTVSGTSCAEVGRE
                     LGIPDMLLAADPGEPDGKQTPARLLLEGERPLPEITEALIGACYLHYGFGATSSAVIE
                     AFRSRISLVRENQTDFKS"
BASE COUNT          199 a          349 c          347 g          169 t
ORIGIN      
        1 cgagatttcc gccgcggccc ggaagaaccc cctgtcggcg gccggcgggc tgctgctcaa
       61 gccggcgctg ggcggtctcc ggcacgacct tcatcccgac acgaccggcg gagcgatcct
      121 gctcggattg cgaaagacag ccgtggtcgg gcatggcagc tccggctccg acggggtggc
      181 aaacgccatc cgcctggccg cccggtcggc ccgggtcgac gcccccgggc tgaccgaaga
      241 gatgcttcac cgggtcggcg ttaaccgcgg ctcgatcgat tccgcaactg acgtctgata
      301 ggaaacggga atgaaccgcg acgaagtaat ggatctggtc cgcaaccacc tggtcaccga
      361 actggaactg ggacccgaac agatcaccga aacggcgagg ttcaaggaag acttcgacgc
      421 cgattcactc gatctctatg aactcgtgat ggaactcgag gaccagtacg ggatcacggt
      481 ctccgaacag gaggccgcgg aaatcaagac cgttggggac gcgcttgact ttgtcgtggc
      541 taaagcggtc gcgtgagcgt gccggggggc acgacccggt aaccccggat tcgggcctgg
      601 cgagcctgat cgaaggcctg ccggaggacc tcaaggttcc ggccctgacc cattcctcct
      661 ggaccagcga gcggacagac tcatacgaac ggcttgccct gctcggtgac agcgtcctcg
      721 gactggccgt cgcggacgag ctttaccgca cccttcccgg gctgagtgcg ggggccctga
      781 ccaagatcct caaccagacg gtcagtggaa cctcctgcgc cgaggtcggt cgcgagctcg
      841 gcatcccgga catgttgctg gccgcggacc cgggcgaacc tgacggcaag cagaccccgg
      901 cccgcctgct gctggagggc gagcggccgc tgccggaaat caccgaagca ttgatcggcg
      961 cctgttacct ccactacgga ttcggagcca cttcgagtgc cgtgatcgaa gccttccggt
     1021 cccggatctc cctggtccgg gagaatcaga cggatttcaa gtcg
//
LOCUS       DAOQFI010000798         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_117936_length_1061_cov_3.318091, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000798 DAOQFI010000000
VERSION     DAOQFI010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117936_length_1061_cov_3.318091"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLE93_11555"
     CDS             <1..753
                     /locus_tag="PLE93_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNH87729.1"
                     /translation="ALGAPPETTDAYLEALAGGVAAAAEEFGAELAGGDVGASPVLFL
                     SVTVIAHIDDETPVVGRKSAAGGEVLISGPIGGAAAGLALLNGLEVSSLSPEDRAYLI
                     DRQLRPNPRLDVAVAASNDGALAMIDISDGLLADLGHVARASGFHIRLPAHRIPIDRG
                     VADVARALGRDPLEFAVNGGEDYELAFIYPGPILRHNYEIFHLPVPVYWIGQSVTPDH
                     GGPLVGVHDGQGFLDLGHGGHDHFRGSDRPSR"
     gene            765..>1061
                     /locus_tag="PLE93_11560"
     CDS             765..>1061
                     /locus_tag="PLE93_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87730.1"
                     /translation="MPKRFSILVLVALLGLAVLAGCGSDSGADSNGDPGSDQDAKPIA
                     TSDADPVLLDSNGSEFNPSKVYAETIDGVVSIRSIFGDIENSPATTSVAGGSGFV"
BASE COUNT          161 a          324 c          349 g          227 t
ORIGIN      
        1 gccctcggcg ctccgcccga aaccacggac gcctaccttg aggcgctggc cgggggtgtc
       61 gccgcggcgg cggaagaatt cggagccgaa ctggccggcg gggatgtggg cgcctccccg
      121 gtcctcttcc tcagcgttac cgtgatcgcc cacatcgacg acgaaacgcc ggtggtcggc
      181 cggaagagtg cggctggagg ggaagtgttg atttctggtc cgatcggcgg ggccgcagcc
      241 gggcttgccc tccttaatgg gctcgaagta tcttcgcttt cacctgaaga tcgcgcctat
      301 ctgattgatc gccagttgcg cccgaatccg agactggacg ttgcggtggc ggcatcgaac
      361 gacggtgcct tggccatgat tgacatcagt gacggacttc tggctgacct cggtcacgtg
      421 gcaagggcgt caggtttcca cattcggctg cccgctcacc gcattccgat agaccggggc
      481 gtcgcagatg tggcgcgggc tttggggcgg gacccccttg aatttgctgt gaacggtggc
      541 gaggattatg aactggcgtt tatctatccc gggccgatcc tgcgccacaa ctacgagatt
      601 tttcatctgc ctgttccggt ttattggatc ggtcaatcgg taacgccgga tcatggtggc
      661 cccttggtcg gggtgcatga cggtcagggg tttctcgatt taggtcatgg tggccacgac
      721 cacttccgcg ggtcggatcg cccttcccgg taggctccgg cgccatgccg aagcgctttt
      781 cgatcctggt tctggttgcc ctgctcgggc tggccgtgct tgccggctgt ggctcggatt
      841 ccggtgccga ttcaaacgga gacccgggaa gcgatcagga cgcgaagccg atcgcgacct
      901 ccgacgccga tccggtcctg ctcgactcga atggttccga gttcaacccg tcgaaggttt
      961 acgccgagac gatcgacggg gtcgtctcga tccgttcgat cttcggcgac atcgagaact
     1021 cgccggccac cacctcggtc gccggcggct ccggattcgt g
//
LOCUS       DAOQFI010000799         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_117945_length_1061_cov_2.936382, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000799 DAOQFI010000000
VERSION     DAOQFI010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117945_length_1061_cov_2.936382"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1061)
                     /locus_tag="PLE93_11565"
     CDS             complement(<1..>1061)
                     /locus_tag="PLE93_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH87731.1"
                     /translation="VPARTEADALDALRNSGQVAYAGRAPVALPPPVTPDFSPGQLHL
                     DMAPTGIGAEYGETEPRVRGGGVTVVDLEYYWTRTHEDLQLPASSDIGGGEYVQYTAF
                     DDEHGTAVFGILNADDNGFGVTGIVPEATMKGISPTMAPDFGYNPAGALTYLADKLSP
                     GDVVLIEQQADGPGPGPSDYVPMEWNQASFDAIRQLGDLGIVVVETGGNGGYDLDSPA
                     MLGRFNRANRDSEAIVVGAGDSVTHAPLWFTSHGSRIDLQGYGNNLVTTGSNGDLQGP
                     GPGEKDIRYTSSFDGTSGAGPVVAGAVAAVLSYLKATGQPPLDADQLVGLLRSTGTPQ
                     ASPELGQIGPLPDIPAAIA"
BASE COUNT          159 a          349 c          377 g          176 t
ORIGIN      
        1 tggcgatcgc cgccggaatg tccggcagcg gtccgatctg ccccagttcc ggactcgcct
       61 ggggagtgcc ggtgctgcgg agcaacccga ccagctggtc cgcgtccagc ggcggctgac
      121 cggtcgcctt caggtaggaa agaaccgcgg cgaccgcgcc cgcgacaacc ggcccggccc
      181 ccgaggttcc gtcgaagctg ctggtgtagc ggatgtcctt ttcgcccgga cccgggccct
      241 ggaggtcacc gttactgccg gtcgtgacca ggttgttgcc gtagccctgg aggtcgatcc
      301 gcgatccgtg cgaggtgaac cagagcgggg cgtgagtgac cgagtccccg gctccgacca
      361 cgatcgcttc ggagtcccgg ttcgcacggt tgaaacggcc gagcatggcg ggggagtcga
      421 ggtcgtagcc cccgttgccc ccggtttcca cgaccacgat tccgaggtcg ccgagctgcc
      481 ggatcgcgtc gaagctcgcc tgattccatt ccatcggcac gtaatcggaa gggccgggac
      541 cgggaccgtc ggcctgctgc tcgatcagca cgacgtcacc gggactcagc ttgtcggcaa
      601 ggtaggtcag agccccggcc gggttgtagc cgaagtcggg tgccatggtc ggggagatgc
      661 ccttcatcgt cgcctcgggc acgatcccgg tcacgccgaa cccgttgtcg tcggcgttga
      721 ggatcccgaa gaccgcggtg ccgtgctcgt catcgaaggc ggtgtactga acgtactcgc
      781 cgccaccgat gtcggaggac gcaggcagct ggagatcctc gtgagtccgg gtccagtagt
      841 actcgaggtc gaccacggtc acgcccccgc cccggactcg tggctcggtc tctccgtact
      901 cggcgccgat accggtggga gccatgtcga ggtgaagctg gcccggactg aaatcgggcg
      961 tgaccggtgg cggaagggcg accggggccc ggccggcata ggcgacctga cccgagttgc
     1021 ggagcgcatc gagggcgtct gcttcggtgc gcgctggtac c
//
LOCUS       DAOQFI010000800         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_118022_length_1061_cov_2.213718, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000800 DAOQFI010000000
VERSION     DAOQFI010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118022_length_1061_cov_2.213718"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLE93_11570"
     CDS             <1..272
                     /locus_tag="PLE93_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNH87732.1"
                     /translation="ETIAVEYQLEYGSDSLEVHSDSIFNGARVLVHDDLIATGGTAQA
                     KIDAIERLGGEVVGCAFLIELEGLGGRHHIAPHKMHTLVTLPAQN"
     gene            complement(358..>1061)
                     /locus_tag="PLE93_11575"
     CDS             complement(358..>1061)
                     /locus_tag="PLE93_11575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH87733.1"
                     /translation="VLMVSRDPEQLAAMREVVRAEGGEVEVVVKDITTDCAGQHIIAA
                     ARQAFGKVDCLVNNAGVAEWKPLEEASEEDFRFQFEMSVIAPLNLMRAAIPEMAERGW
                     GRVVNVSSTAGKRPSSMMPDYSVGKAAMLSLSRQFAERHAAEGVMVNAICPGPTASDL
                     WMAEGGLLDQSKEAAGHASREEALKAAGGKRPIGRLATPEEIASAIVFLCSERASYVS
                     GAAWGVDGGTVPIII"
BASE COUNT          185 a          375 c          319 g          182 t
ORIGIN      
        1 ccgagacgat cgcggtcgag tatcagctcg agtacgggtc ggactcgctt gaagtccatt
       61 ccgactcgat cttcaacggg gcgagggtgc tggtccacga tgacctgatc gcgaccggcg
      121 gtaccgccca ggcgaagatc gacgcgatcg agcggctcgg tggcgaggta gtcggctgcg
      181 cgttcctgat cgaactcgaa ggtctcggcg gccgccacca catcgccccc cacaaaatgc
      241 acaccctcgt aaccctcccc gcccagaact gatcccacca cctaacggag ccctcccatc
      301 catccatttc ttctgtggga gggcgggttc aatttgcggc ccgaacgggc cgccccttta
      361 aatgatgatc gggacggtgc cgccgtccac gccccaagca gctcccgaga cataggacgc
      421 acgctccgag cagaggaaga caatcgccga ggcaatctct tcgggcgtgg cgagccgacc
      481 gatcggccgc ttcccgccgg ccgccttcag ggcttcctcc cggctggcat gtccggccgc
      541 ttccttcgac tggtcgagca ggccgccttc ggccatccat agatccgagg cagtaggacc
      601 ggggcagatc gcgttgacca tcacgccctc ggccgcgtgg cgctcggcga actggcgcga
      661 aagcgagagc atcgccgcct tgcccaccga gtagtcgggc atcatcgagg agggacgctt
      721 gccggcggtc gaggaaacgt tgacgacccg gccccagccg cgctcggcca tctccgggat
      781 tgctgcccgc atcaggttca gcggcgcgat aacgctcatc tcgaactgga atcgaaagtc
      841 ttcctctgag gcttcctcga gcggcttcca ctcggctacc ccggcgttgt tgaccaggca
      901 atcgaccttg ccgaaggcct gcctggcggc ggcgatgatg tgctgcccgg cgcagtcggt
      961 ggtgatgtcc ttgaccacca cctcgacttc accgccctcg gcccggacca cttcccgcat
     1021 cgccgcgagc tgttcgggat cacgcgaaac catcagtacc t
//
LOCUS       DAOQFI010000801         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_118375_length_1059_cov_2.630478, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000801 DAOQFI010000000
VERSION     DAOQFI010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118375_length_1059_cov_2.630478"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..412)
                     /locus_tag="PLE93_11580"
     CDS             complement(209..412)
                     /locus_tag="PLE93_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87734.1"
                     /translation="MLKPHRVVIDKVSSYTDEAAAFMRRRRHHRREFVRIRFQSGAES
                     DLPSGAPEADRIAAAARRLIELG"
     gene            complement(405..848)
                     /locus_tag="PLE93_11585"
     CDS             complement(405..848)
                     /locus_tag="PLE93_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87735.1"
                     /translation="MADVNGKSNESGANLPGDDGLAAASARVLENLAELEPQLIAGGI
                     FDSERNPVATTSDSPTWAREAAALLEALEAGSGEEEFDSAHVASTEGEVFAVTESGLS
                     LVAVTGRFVLASLTSYDMRMALRDVVRSNPRNPASEAGNGGSDDA"
     gene            869..1039
                     /locus_tag="PLE93_11590"
     CDS             869..1039
                     /locus_tag="PLE93_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87736.1"
                     /translation="MSLAQASWIIVVVICAIATAVTAIAGYAGYAATIAAVGVAASVN
                     LLPHLGAHSTEN"
BASE COUNT          178 a          353 c          327 g          201 t
ORIGIN      
        1 cagggtgtcg gtaatcgcgt ccaggacggc gtcgacggcg tcaggcgcgg ccttcttgct
       61 tccgatgcgc tcgtcaccgg cgaccttctc aagaaactct gctttggtca cgtcagtaat
      121 cctcctcggt ttagggaatg gcggaaccgt aacaccgcat gcggacaccg tttctgccac
      181 ctttggcttc agggcgggga tgcagaactc agccgagttc gataaggcgg cgcgccgcgg
      241 cggcaatccg gtcggcttcg ggggccccgg agggcagatc cgactcggct ccgctctgga
      301 agcggattcg gacgaattcg cggcggtgat gccggcgccg gcgcatgaaa gccgccgctt
      361 cgtcggtgta ggaactcacc ttgtcgatca ccacgcggtg aggcttaagc atcgtctgat
      421 cctccgtttc cggcttcaga agcgggattg cgaggattcg accgaaccac gtcccgcagc
      481 gccatcctca tgtcatagga ggtcagcgag gccagaacga agcgtcccgt cacggcgacc
      541 agcgagagcc cggattcagt gaccgcaaag acttctccct cggtcgaggc gacgtgagcg
      601 gagtcgaact cttcctcccc ggaaccggct tcgagagcct caagcagcgc cgccgcttcc
      661 ctggcccagg tcggggaatc cgaagtggtc gcaaccggat ttcgctccga atcgaagatt
      721 ccgccggcga tcagctgcgg ttcgagctcg gccaggtttt cgagcacccg ggcggatgct
      781 gccgccaggc cgtcgtcacc gggcaggttc gcgcccgatt cattgctctt gccgttcaca
      841 tccgccacgg tgtaggaaca taccgtccat gtcccttgct caggcctcct ggatcatcgt
      901 cgtggtgatc tgcgcgatcg ccacggcggt caccgcaatc gccggttacg ccggttacgc
      961 cgcgacgatt gccgccgtcg gagtggccgc ctcggtcaat ctgctccccc acctcggcgc
     1021 ccactcgacc gaaaactgat tcccgggcta gttcccgat
//
LOCUS       DAOQFI010000802         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_118416_length_1059_cov_2.231076, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000802 DAOQFI010000000
VERSION     DAOQFI010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118416_length_1059_cov_2.231076"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLE93_11595"
     CDS             complement(<1..331)
                     /locus_tag="PLE93_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87737.1"
                     /translation="MSEKGSRLEGGALTGEVIDSSRKVLTGGRNGTGVGLQQYFTPPQ
                     VGELLAKVFNRDLPTLDLTAGDGGLLEWVSDEHRYGIEIDRDQVSNGNYTKHAITGDI
                     QKVYPLLR"
     gene            complement(436..>1059)
                     /locus_tag="PLE93_11600"
     CDS             complement(436..>1059)
                     /locus_tag="PLE93_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87738.1"
                     /translation="EDPETEKDLVIMRFETGTVFTRDQVDPIEGKALSVDPPKPEPVT
                     GDSHQHLIGKLTGFAEKTGYKVKELDPEESPGCHGYHDKEGMVIAYRPEQPKNAIVRV
                     LVHELIHSLGTSYQKFGRARAETITDSATFMVCRMLGLDVEKASVPYVAGWGGGDVKK
                     ISDDLNLMNQLANQIIDGAKLESDLALSEAESSMRQNKTNSATAKTA"
BASE COUNT          159 a          306 c          316 g          278 t
ORIGIN      
        1 ctcgaaggag cggatacacc ttctggatgt cgcctgtgat cgcgtgcttg gtgtagttcc
       61 cgttgcttac ctggtcacgg tcgatttcga tgccgtaacg gtgttcgtcg ctcacccatt
      121 cgagcaggcc gccgtcgccg gcggtcaggt caagggtggg caggtcgcgg ttgaatactt
      181 tggccagcag ctccccgacc tggggtgggg tgaagtactg ctggaggccc acaccggtgc
      241 cgtttcggcc accggtgaga accttccttg acgagtcgat gacttcgcct gtcagggcgc
      301 caccttcaag gcggctgcct ttttcactca tgacgtttcc tttcgtttca actccgcagg
      361 agctacctgc gaaacagatt ccgtctttcg acggactaca gattttaggt aatgctcagg
      421 cgtaaaagcc tgttttcatg cggttttcgc ggttgcgctg ttggtcttgt tctggcgcat
      481 tgacgattcg gcctctgaca gcgccaggtc gctttcgagt ttcgcgccgt cgatgatctg
      541 gttggccagc tggttcatca ggttgaggtc gtcactgatc ttcttgacgt ctcccccgcc
      601 ccatccggcg acatacggca ccgatgcttt ctccacgtcg aggccgagca tccggcagac
      661 catgaacgtg gccgagtcgg tgatcgtttc tgccctggct ctaccgaatt tttggtagga
      721 ggtgccaagg gaatggatca gttcatggac gagaacccgg acgatcgcgt tcttcggctg
      781 ctctggccgg taggcgatca ccatgccttc cttgtcgtgg tagccgtggc agccggggct
      841 ttcttccggg tccagttcct tgaccttgta cccggttttt tccgcgaacc cggtcagctt
      901 gccgatcagg tgctggtggc tgtcaccggt caccggttcg ggtttcggcg gatcaactga
      961 cagggctttc ccttcgatcg ggtcgacctg gtcgcgggtg aacacggtcc cggtttcgaa
     1021 tcgcatgatc accaagtcct tttcggtttc gggatcttc
//
LOCUS       DAOQFI010000803         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_118642_length_1058_cov_2.258225, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000803 DAOQFI010000000
VERSION     DAOQFI010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118642_length_1058_cov_2.258225"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..797)
                     /locus_tag="PLE93_11605"
     CDS             complement(333..797)
                     /locus_tag="PLE93_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNH87739.1"
                     /translation="MADELLAKALAAISRKDRTEAEMRAWLAAREAGPEEIDQVIDYL
                     VENLALDDRRFAFGYTSDKRQLAGWGRDRIRENLLRRGIDRYLVEEALNQSAQGGEEV
                     ETEVDRAVRVLGEKGADLSDDRGRQRALGLLARRGYDAEEAYAAIRIAGRAA"
     gene            complement(826..>1058)
                     /locus_tag="PLE93_11610"
     CDS             complement(826..>1058)
                     /locus_tag="PLE93_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87740.1"
                     /translation="SFFSYGETRLGQGRNNSKQFLAENPEIAQEIEDRILANLGISRD
                     GSPIESSAPEGVDAETGEVIETAGGDAELKAA"
BASE COUNT          161 a          380 c          314 g          203 t
ORIGIN      
        1 gcaaaaaatc aatttatttt ggcgcgtgcg aggagaatcg cctagggtcg atgctgggag
       61 gtgctccgtg tcaagcacgc gcgatctgtt cgctgagaca gcgacattaa tcttcccgga
      121 ccggttccgg atgccggccc tggcgccgag ccgttggttg ccggcccgct ggtctctttc
      181 ccgccttcag cctccggttt cggagaccgg ttccgcagcc aggccgggat gatcgactcg
      241 aacgccatgc ctccctcaag ggtagccgac gcttcaactc cccttttcga cagcccccgc
      301 aggcaagatt cagtaccggt ccggtgctcc gctcaggccg cgcgaccggc gatgcggatc
      361 gcggcgtagg cctcctcggc gtcatatccg cgccgcgcga gcagcccgag ggcacgctgc
      421 cttccccgat cgtcggagag gtcagcgccc ttctcgccga gcacccgcac tgccctgtcc
      481 acttcggttt cgacctcttc cccgccctga gcggactggt tcaaagcctc ctcgaccagg
      541 taacggtcga tgccccgccg gagcaggttc tcccggatcc ggtcacggcc ccagccggca
      601 agctgtcgct tgtcactggt gtatccgaaa gcaaaccggc ggtcgtcaag cgcaaggttc
      661 tcgaccagat agtcgatcac ctggtcgatc tcttcgggcc cggcttcgcg cgcggcgagc
      721 caggcccgca tttccgcctc ggtccggtcc ttgcggctta tcgccgcgag ggctttcgca
      781 agcagctcgt cagccatcaa ccggacctgg ccgggctgac cggcctcagg ccgccttcag
      841 ttcggcatcg cccccggcag tctcgatcac ctcaccggtc tcggcatcca cgccttccgg
      901 cgccgaactc tcgatcggcg agccgtcccg cgaaatcccg aggttggcga ggatccggtc
      961 ctcgatctcc tgcgcgatct ccgggttctc ggccagaaac tgcttcgagt tgttgcgacc
     1021 ctgcccgagc cgggtttctc cgtaggagaa gaaggaac
//
LOCUS       DAOQFI010000804         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_118778_length_1057_cov_3.178643, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000804 DAOQFI010000000
VERSION     DAOQFI010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118778_length_1057_cov_3.178643"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLE93_11615"
     CDS             <1..236
                     /locus_tag="PLE93_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNH87741.1"
                     /translation="LYRDELKKVERDETNGRTSYRIYGYEPGLDDEPGTDIGAIARGR
                     LTVTPLSLDWTHHDEIEGLRGRDFERLLGAATS"
     gene            233..>1057
                     /locus_tag="PLE93_11620"
     CDS             233..>1057
                     /locus_tag="PLE93_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNH87742.1"
                     /translation="MSEEEAAAGEPSPDDASRIAELREVLNHHIVLYYEKDDPEISDE
                     EYDRLFAELQELEGRNPELITGDSPTQRVGAPASTSFAPVRHAVPMLSLANARTEEEL
                     TAWENRIRNHLLRRDVDPGEITFVTEPKIDGLAISLTYENGRLTKGATRGDGQVGEDV
                     TLNIETIRTVPKQIDDAPPLIEVRGEVYLPIADFAELNQKRVDAGLPAFANPRNSAAG
                     SIRQNDPKAAAERPLAVWSYGIGAMDGIRFASHSEEIEWLRDHGFHVNPDIAVHDSI"
BASE COUNT          212 a          364 c          339 g          142 t
ORIGIN      
        1 ggctctaccg cgacgagctg aagaaggtgg agcgggacga gaccaacggc cgtacctcct
       61 accggatcta cggctacgag ccgggcctcg acgacgagcc cggcaccgac atcggcgcga
      121 ttgcccgcgg ccggctcacg gtcaccccgc tcagcctgga ctggacccat cacgacgaga
      181 tcgaaggcct gcgcggccgc gatttcgaac ggctgctggg agcggccacc tcttgagcga
      241 ggaagaggca gcggccgggg aaccgtcacc cgacgacgcc agtcggatcg ccgaactgcg
      301 tgaagtcctg aaccatcaca tcgtcctcta ttacgagaag gacgacccgg agatctccga
      361 cgaggaatac gaccggcttt tcgccgaact ccaggaactc gagggccgga acccggaact
      421 gatcaccggt gactcgccga cgcagcgggt cggcgccccg gcttcgacca gcttcgcccc
      481 ggtcaggcat gcggtcccga tgctttctct ggccaacgcc agaaccgaag aagagctgac
      541 cgcctgggag aacaggatcc ggaaccacct gctgcgccgt gacgtggacc cgggcgagat
      601 caccttcgtc accgaaccga agatcgacgg cctggcgatc tcgctcacct acgagaacgg
      661 gagactgacc aagggcgcga cccgcggtga cggtcaggtc ggggaggacg tcaccctcaa
      721 catcgagacg atcaggacgg tgccgaagca gattgacgac gccccgcccc tgatcgaggt
      781 tcggggcgag gtctacctgc cgatcgccga tttcgccgag ctcaaccaga agcgggttga
      841 cgccggcctg cccgccttcg ccaacccgcg caactccgcg gccggatcga tccggcagaa
      901 cgacccgaag gccgcggccg agcgtccgct cgcggtctgg tcatacggga tcggggcgat
      961 ggacgggatc aggttcgcca gccactccga agagatcgag tggttgcggg accacggctt
     1021 ccacgtcaac cccgacatcg ccgtccacga ctcgatc
//
LOCUS       DAOQFI010000805         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_118987_length_1056_cov_2.937063, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000805 DAOQFI010000000
VERSION     DAOQFI010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118987_length_1056_cov_2.937063"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLE93_11625"
     CDS             complement(<1..206)
                     /locus_tag="PLE93_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87743.1"
                     /translation="MCDGSEMNRDDERIIEGMRRQSEGISKSGAGGAANLERLGWKAG
                     FGTGATMALIGTSQPLAGFLTKATL"
     gene            313..714
                     /locus_tag="PLE93_11630"
     CDS             313..714
                     /locus_tag="PLE93_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87744.1"
                     /translation="MTACGSDDSSSDDSSESITKAEFVAQANEICAESNKTIDAAEKE
                     AFSGGQPTQADVDSFINDTVLPQVESQINDIEALPVPEGEEDQISAILDAANKALEEG
                     KSDPSSLQGNGDPFAEANKLANAYGMTECGG"
     gene            complement(771..>1056)
                     /locus_tag="PLE93_11635"
     CDS             complement(771..>1056)
                     /locus_tag="PLE93_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87745.1"
                     /translation="AIPLAAAGASGRRQFWMAVASSVPQPIGAVIAYLLVQEISALLP
                     VSFGFAAGAMLALTLLEILPEAWIENPRQTFLGLATSIPLMVALSLALGV"
BASE COUNT          209 a          336 c          344 g          167 t
ORIGIN      
        1 agcgtcgcct tcgtcaggaa ccctgccagt ggctggctgg tcccgatcag tgccatcgtc
       61 gccccggtgc cgaacccggc cttccagccg agccgctcca ggttcgcggc cccacccgca
      121 cccgactttg aaatgccttc ggactgtcgc cgcatcccct cgatgattcg ctcgtcatcc
      181 cggttcattt cactcccgtc gcacatttgc ggttagtttc aactgagacc agtcaaccga
      241 agggggagta tgagtgcatt gaaggttggg aagatcggcg tctgttcgtt gctccttatg
      301 gtctcgctgg gcgtgacagc ctgcgggtcc gatgacagct cgtccgacga ttcttcggaa
      361 tcgatcacca aggcggagtt cgtcgcccag gcgaacgaga tctgcgccga gagcaacaag
      421 acgatcgacg cggccgagaa ggaagcgttc tccggcggcc agccgaccca ggcggatgtc
      481 gactcgttca tcaatgacac cgtgctgcct caggtggagt cccagatcaa cgacatcgag
      541 gcgcttccgg ttccggaggg cgaagaggac cagatcagcg cgatcctcga tgcggccaac
      601 aaggctctcg aggagggcaa gtcagatcct tcctccctgc aaggcaacgg ggatccgttc
      661 gcggaagcga acaaactcgc caacgcatac ggcatgaccg aatgcggagg ctgaccatcc
      721 gataggtgcg agagcggccg gcctcgtcag gcgaaggcgc agccggtcgc tcagaccccg
      781 agcgccaggc tcagggcgac catcaaaggg atcgaggtgg ccaggccgag aaaggtctgg
      841 cgggggtttt cgatccaggc ctcggggagg atctcgagca gggtcagggc gagcatcgcc
      901 ccggcagcga agccgaaaga gaccggcagc agggcggaaa tctcctggac cagcaggtag
      961 gcgatcaccg caccgatcgg ctgcggcacg ctgctcgcga ccgccatcca gaactggcgg
     1021 cggcccgagg cacccgcggc ggcaagcggg atcgcg
//
LOCUS       DAOQFI010000806         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_119369_length_1054_cov_4.863864, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000806 DAOQFI010000000
VERSION     DAOQFI010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119369_length_1054_cov_4.863864"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLE93_11640"
     CDS             complement(<1..480)
                     /locus_tag="PLE93_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH87746.1"
                     /translation="MSEPLLSVRDLEVSFRTEGGLLQAVGGVSFDLQRGETLSIVGES
                     GCGKSVTAMTLMGLTSGPNTEFKGSVKLDGQELLGASNEVMRPIRGDRMSMVFQNPMT
                     SLNPVHRIGNQIVEQIRAHRDVSKKEAMARAIELLDAVGVPDAAARARAYPHEFSGGM
                     "
     gene            complement(477..>1054)
                     /locus_tag="PLE93_11645"
     CDS             complement(477..>1054)
                     /locus_tag="PLE93_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH87747.1"
                     /translation="LVGISMPVFWLGALMNQYLGFELTKKLGFELFPNGGYVEFSQSP
                     WEWFHHLILPWIALSVLFIGFYSRVLRANILETMDEDYVRTARAKGLSERRVMLSHVL
                     RNSMISVVTLWGLDFAVVVGGGAILTETVFNLQGVGQYAADAIGQLDQPPIMAVTMFG
                     AFFVVFFNAVIDIVYAALDPRIRLQDGDTAL"
BASE COUNT          199 a          341 c          333 g          181 t
ORIGIN      
        1 catgccgccc gagaactcgt gcgggtaggc ccgggcccgg gcggctgcgt ccggcacacc
       61 gaccgcgtcg agcagctcga tcgccctggc catcgcttcc ttcttgctga cgtcgcgatg
      121 ggcccggatc tgttcgacga tctggttgcc gatccggtgg accggattca gggaggtcat
      181 cgggttctgg aagaccattg acatgcggtc gccccggatc ggccgcatca cttcgttgct
      241 ggcaccgagc agttcctggc cgtcgagctt gaccgagccc ttgaactcgg tgttgggacc
      301 gctggtcagg cccataaggg tcatcgcggt taccgacttg ccgcagcccg actcgccgac
      361 gatcgagagc gtctcgccgc gctggagatc gaacgaaacg cccccgacgg cctggagcaa
      421 cccgccctcg gtgcggaagc tgacctcgag gtcgcgcacg ctgagcagcg gttcactcac
      481 agggctgtgt ccccgtcctg cagacggata cgcgggtcca gtgccgcgta gacgatgtcg
      541 atcacggcgt tgaagaagac cacgaagaag gcgccgaaca tggtcaccgc catgatcggc
      601 ggctggtcta gctgcccgat cgcgtcggct gcatactggc cgacgccctg gaggttgaag
      661 acggtttcgg tcaggatcgc gccgccaccg accaccaccg cgaagtcaag tccccagagg
      721 gttacgaccg agatcatcga gttgcggagg acgtggctga gcatcaccct tcgctccgac
      781 agccccttcg cccgggcggt ccggacgtag tcctcatcca tcgtttcgag gatgttcgcc
      841 cgaaggacgc gggagtagaa gccgatgaac aggaccgaga gcgcgatcca gggcaggatc
      901 agatggtgaa accactccca ggggctttgc gagaactcga cgtacccccc attcggaaat
      961 aactcgaagc cgagtttctt ggtcaactcg aacccaaggt attggttcat cagcgctccg
     1021 agccagaaca ccggcatcga gattccgacc agcg
//
LOCUS       DAOQFI010000807         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_119871_length_1052_cov_3.456369, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000807 DAOQFI010000000
VERSION     DAOQFI010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119871_length_1052_cov_3.456369"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1052
                     /locus_tag="PLE93_11650"
     CDS             <1..>1052
                     /locus_tag="PLE93_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87748.1"
                     /translation="AGIRVYAWESSGEEVTGFPVTSDLGKCGAALESQPLSHPKCGFI
                     ASPAIARLQGPDAAPDIVVPGLDGYLYAFSGQGDAVPGYPVRLVDPDIPAGEQMLAES
                     INSPAVGDLNGDGRDDAVVATNETYDAQPPDFESISGGVVGSALTDILANAAGGSERV
                     YAVDSRGNGFEGNGGSPYLDGWPIKLNGAIQSTLPLIGPGQDPAITMVGGEPRVVASA
                     TGSATIGIYKPDGTVALNVEQAVPGAASVADGAIGTGAVNLFESASLGKLTPGATNPS
                     IVKYGLGLPGILNLLLVGQNLPYNHLIGAYDTATGLSAPSFPRVTEDFQFLSSSTIAN
                     LNGSQLSSQVLAGTGLG"
BASE COUNT          170 a          365 c          348 g          169 t
ORIGIN      
        1 gccggaatca gggtctacgc ctgggaatcc agcggtgaag aagtcaccgg gttcccggtc
       61 acgtcggacc tcggcaagtg cggtgccgcg ctcgaaagcc aaccgctttc gcatcccaag
      121 tgcggattca tcgccagccc cgcgatcgcg cgacttcagg gtccggacgc ggcgcccgac
      181 atcgtcgtcc ccgggctcga cggttacctc tacgcgttta gcggccaggg cgacgccgtt
      241 ccggggtacc cggtgcgtct ggtcgacccc gacatcccgg cgggcgagca gatgctggcc
      301 gaatcgatca actcgcccgc ggtcggtgac ctgaacggcg acggtcgcga cgatgccgtg
      361 gtcgcaacca acgagaccta tgacgcccag ccgccggatt tcgagagcat ctccggcggc
      421 gtggtcgggt cggccctcac cgacatcctc gccaacgcgg cgggaggttc cgagcgggtc
      481 tacgccgttg actcacgcgg caacggtttt gaaggcaacg gcggaagccc ctatctggac
      541 ggctggccga tcaagctgaa cggcgcgatc cagtccaccc tgcccctgat cgggccggga
      601 caggatccgg cgatcacgat ggtgggcggt gaaccgcggg tcgtcgcgtc ggccacgggt
      661 tcggccacga tcggcatcta caagccggac ggcaccgtgg ccctgaacgt cgagcaggcg
      721 gtcccgggag cggcttcggt ggccgacggg gcgatcggaa cgggtgcggt caatctcttc
      781 gaatcggctt cgctcgggaa gctcacgccg ggcgccacga atccgagcat cgtcaagtac
      841 ggtctcggcc tgccgggcat cctgaacctg ctgctggtcg gccagaacct gccctacaac
      901 catctgatcg gggcctacga cacggcgacc ggcctttcgg ccccgtcctt cccccgggtt
      961 accgaggact tccagttcct ttcctcctcg acgatcgcca acctgaacgg cagccagctt
     1021 tccagccagg tgctggccgg gaccgggctc gg
//
LOCUS       DAOQFI010000808         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_120561_length_1049_cov_3.126761, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000808 DAOQFI010000000
VERSION     DAOQFI010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120561_length_1049_cov_3.126761"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1049
                     /locus_tag="PLE93_11655"
     CDS             <1..>1049
                     /locus_tag="PLE93_11655"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNH87749.1"
                     /translation="SKSRAADQPFKIDFDQSSMQIGLLSDLPLGDLATTGSLDGTIDE
                     EGNVTIPKGNFILPEIGITDPIAIKVFMGVESDATGTYDANTGELVLNAKAGVWFSVD
                     LQALLSTLGTLGVQLPGGSGIIDTILGQVTDLTCGFSPMDVTFTTGETSQGSGAPFSE
                     GPLGPGALTAEWSQLGPFAGKTKILGLVDACQAIKMYAPTLLESALGSSGIDLASLGL
                     GDIDLASLLDNLDNLNLGPSSLTLTRTLDESPDPPAAEPVLKMSVKGPNRKVKAGSKA
                     EFTVKVKNSGQGDATGVRVCLKSSGPVTVQGSRCRTLGKVMAGATQTRHFKVKAKKGK
                     KKSAKISFSLKSSNG"
BASE COUNT          198 a          358 c          325 g          168 t
ORIGIN      
        1 cctcgaagtc ccgggctgcg gatcagcctt tcaagattga cttcgaccag agcagcatgc
       61 agatcggcct gctcagcgat ctgccgctcg gcgacctcgc gaccaccggc tccctcgatg
      121 ggacgatcga tgaggagggc aacgtcacca tccccaaggg caacttcatc ctccccgaga
      181 tcggcatcac cgacccgatc gcgatcaagg tgttcatggg tgtcgagagc gacgccaccg
      241 ggacctacga tgccaacacc ggtgaactgg tcctcaacgc caaggcgggg gtctggttct
      301 cggtcgacct ccaggccctg ctctccacgc tcggcacgct gggagtccag ctgccggggg
      361 gttcgggaat catcgacacg atccttggcc aggtgaccga cctgacctgt gggttctcgc
      421 cgatggacgt caccttcacc accggtgaga cctcgcaggg ttccggcgcc ccgttcagcg
      481 aggggccgct cgggcccggt gcgctgaccg ccgagtggtc gcagctcggg cccttcgcag
      541 gcaagaccaa gatcctcggt ctggtcgacg cctgccaggc gatcaagatg tacgcgccca
      601 ccctgctcga gagtgccctt ggcagctccg gcatcgatct cgccagcctc gggctcggtg
      661 acatcgacct ggcttccctg ctcgacaacc tcgacaacct gaatctcggc cccagctctc
      721 taaccctgac ccggacgctc gacgagagcc cggacccgcc cgcggcggag ccggtgctga
      781 agatgagcgt caagggaccg aaccgcaagg tcaaggccgg cagcaaggcg gagttcaccg
      841 tcaaggtcaa aaactcgggt caaggtgacg cgaccggcgt ccgggtctgc ctgaagtctt
      901 ccgggccggt gaccgtccag ggaagccgct gccggaccct cggcaaggtg atggccggtg
      961 ccacccagac ccgtcatttc aaggtgaagg cgaagaaggg caagaagaag tccgccaaga
     1021 tcagcttctc gctcaagtcg tcaaatggc
//
LOCUS       DAOQFI010000809         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_120787_length_1048_cov_3.477341, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000809 DAOQFI010000000
VERSION     DAOQFI010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120787_length_1048_cov_3.477341"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..>1048
                     /locus_tag="PLE93_11660"
     CDS             289..>1048
                     /locus_tag="PLE93_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNH87750.1"
                     /translation="MSTGANIISPSDPGFAEAKFAYDLHDQKPAAICLPTTVAEVQQA
                     VRWARDEGLRIANQATGHVAVALPDLEDALLLKPQIDTEPVVDPAAGYCRVGAGTRWQ
                     PVVEAAAAHGMAAMHGSSPTVGVVGYHLGGGLSFYSREHGLACNKVRAIEVVTADGEL
                     ARIDRENEPDLFWALRGGGGLFATVTAIEFDLLPVGEVYGGASFWPVETAEAALSTWL
                     DWTRTAPESVTSTFRIMNLPPFEVVPEPIRGKQLV"
BASE COUNT          169 a          356 c          357 g          166 t
ORIGIN      
        1 ggggtggccg aggcgaggaa actcccgccg ggatgggccg ccagctccgc cagggcgagc
       61 ttctgccagc tccgaaggcc ccgctcctgc ggccacgggg ggaggttccg gcccggtcca
      121 ggggttggtt cgggctgtgg cgcgggctgg gtcaaggcag cggaacctac cctgaccgac
      181 ggtcggaacg ccgtccgatc cacccgtgat atgagggccg atttcccctt ggcctcaacc
      241 tgaatcaggc acttgcgaag aggttgtccg gtgggctagt ttgggcgaat gagcaccggc
      301 gcgaacatca tcagtccatc ggaccccggc ttcgcagaag cgaaattcgc ctacgacctt
      361 catgaccaga agcctgccgc gatctgcctg ccgacgacgg ttgcggaggt acagcaggcc
      421 gttcgctggg cccgggacga gggccttagg atcgccaacc aggcgaccgg tcacgtcgcc
      481 gtggccctgc ccgacctcga ggacgccctg ctgctcaaac cgcagatcga caccgaaccg
      541 gtcgtcgatc cggccgccgg ttactgccgg gtcggggccg gaacccgttg gcagccggta
      601 gtggaagccg ccgccgctca cggcatggcc gccatgcatg gctcctcgcc taccgtaggc
      661 gtggtcggct accacctcgg cggcggcctc agtttctaca gccgcgagca cggactcgcc
      721 tgcaacaagg tgcgggcgat cgaggttgtc accgccgacg gggaactggc ccggatcgac
      781 cgcgaaaacg agcccgacct gttctgggcg ctccgcggcg gcggcgggct cttcgccacg
      841 gtgaccgcga tcgaattcga cctgcttccg gtcggcgagg tctacggcgg ggcgagcttc
      901 tggccggtcg aaacggccga ggcggcactc tcgacctggc tcgactggac ccggaccgct
      961 cccgaatcgg tgacctccac tttccgcatc atgaatctgc ctcccttcga agtggtgccc
     1021 gaaccgattc ggggaaagca actggtgg
//
LOCUS       DAOQFI010000810         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_121051_length_1047_cov_2.849798, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000810 DAOQFI010000000
VERSION     DAOQFI010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121051_length_1047_cov_2.849798"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1047
                     /gene="aspS"
                     /locus_tag="PLE93_11665"
     CDS             <1..>1047
                     /gene="aspS"
                     /locus_tag="PLE93_11665"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNH87751.1"
                     /translation="DEDLRADRQPDFTQLDIEMSFVTGEDVIEVNERLIRHVLAEMGV
                     EVEIPFERMCYDEAIGRFGSDKPDLRYGYELTDLTEILRGTEFKAFGGVIEGGGQVKG
                     INFGQRELSRAQLDGLIDDAKELGAKGLVWAFREGDGWRAPIAKFLSEGELKNLNEAM
                     GAEEGDLMLVAADQPAMVAAILGGLRTRLAERWDLIPADAGNRFCWIVDWPLFEFNEE
                     EDRWDALHHPFTAPKGDFDPENPGEARAEAYDLVWNGVEIGGGSIRINQPELQSQIFR
                     ALGISDEEAEERFGFLLEALRYGAPPHGGIAYGVDRFAALLAGVESIRDVIAFPKTAS
                     GGDPLTGAPAPIDDV"
BASE COUNT          187 a          346 c          348 g          166 t
ORIGIN      
        1 gacgaggacc tgcgcgccga ccgccagccc gacttcaccc agctcgacat cgagatgagt
       61 ttcgtcaccg gcgaagacgt gatcgaggtc aacgagcgcc tgatccggca tgtgctggcc
      121 gagatggggg tcgaagtgga gatccccttc gagcgcatgt gttacgacga ggcaatcggc
      181 cgcttcggct ccgacaaacc ggaccttcgc tatggctacg aactgaccga cctgaccgaa
      241 atcctccgcg gcaccgaatt caaggccttc ggcggcgtga tcgaaggcgg agggcaggtc
      301 aagggcatca acttcggcca gcgcgagctt tcccgggccc agctcgacgg cctgatcgac
      361 gacgccaagg agctcggggc caagggcctg gtctgggcct tccgtgaggg cgacggctgg
      421 cgggccccga tcgcgaagtt cctctctgaa ggcgaactga agaacctgaa cgaggcgatg
      481 ggtgccgaag aaggcgacct gatgctggtc gccgccgacc agccggccat ggtcgcggcc
      541 atcctcggcg gccttcgcac ccggcttgcc gagcgctggg acctgatccc ggccgatgcc
      601 ggcaaccggt tctgctggat cgtcgactgg ccgcttttcg aattcaacga ggaagaagac
      661 cgctgggatg ccctgcacca tcccttcacc gcgccgaagg gcgatttcga tccggaaaac
      721 ccgggcgagg ccagggccga ggcctatgac ctggtctgga acggggtcga gatcggcggc
      781 ggttcgatcc gcatcaacca gccggaactc cagtcacaga tcttcagggc cctgggaatc
      841 tccgatgagg aagccgagga gcgattcggc tttctgctcg aggccctccg ctacggggca
      901 ccgcctcacg gcggcatcgc ctacggggtc gatcgcttcg cggcgctgct ggccggggtc
      961 gaatcgatcc gtgacgtgat cgccttcccc aagaccgctt ccggcggcga tccgctgacc
     1021 ggcgcgccgg ccccgatcga cgacgtc
//
LOCUS       DAOQFI010000811         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_122102_length_1042_cov_3.425532, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000811 DAOQFI010000000
VERSION     DAOQFI010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122102_length_1042_cov_3.425532"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLE93_11670"
     CDS             complement(<1..727)
                     /locus_tag="PLE93_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNH87752.1"
                     /translation="MSETTTAPIDLFEKIKNHERREQLEAANEGGLNPYFRLLTSQAG
                     PVVEMEGRETIMLGSNNYLGLTGDERVKNAAREALDQYGTGVTGSRLLNGTTPIHVEL
                     EKELAGWMNTEDAIVFSTGYQANVGCLQAILGPADTVICDSGDHASLLDGMKLSGAKL
                     RPFRHNQTDKLEKMLQRAQGDGGGILVVVDGVYSMEGDLPDLPRIVELSKHYGARIMV
                     DEAHGVGVLGERGAGATELFGLED"
     gene            complement(798..>1042)
                     /locus_tag="PLE93_11675"
     CDS             complement(798..>1042)
                     /locus_tag="PLE93_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNH87753.1"
                     /translation="SSNLTGITDRLEERGLVKRTLDPDDRRVRLLVLTAKGRKVQGRV
                     NAFLRQPPLSMEALDNEELETLTRLLAKLDPAGLPG"
BASE COUNT          146 a          316 c          380 g          200 t
ORIGIN      
        1 cgtcttcgag accgaagagt tcggtcgcgc cggcaccgcg ctcgcccagc acgccgaccc
       61 cgtgggcctc gtcgaccatg atccgggcgc cgtaatgctt cgatagttcg acgatccggg
      121 gcaggtcggg caggtcgcct tccatcgagt agacgccgtc gaccacgacc aggatgccgc
      181 cgccgtcgcc ctgggcccgc tggagcatct tttccagctt gtcggtctgg ttgtggcgga
      241 aggggcggag cttggccccg gaaagcttca tgccgtcaag cagcgaggcg tggtcgccgg
      301 agtcgcagat caccgtgtcg gccgggccga ggatcgcctg caggcagccg acgttggcct
      361 ggtatccggt cgagaagacg atcgcgtcct cggtgttcat ccagccggcc agttcctttt
      421 cgagttcgac gtggatcggc gtggtgccgt tcaggaggcg cgagccggtg accccggtgc
      481 cgtactggtc gagggcctcg cgggccgcgt tcttgacccg ttcgtcgccg gtcagcccga
      541 ggtagttgtt ggaaccgagc atgatcgttt cccggccttc catttcaacc accgggccgg
      601 cctgggaggt gagcaggcgg aagtaggggt tgaggccgcc ttcattggcg gcttcgagct
      661 gctctcggcg ctcgtggttc ttgatcttct cgaagagatc gatcggtgcg gtcgtggtct
      721 cggacatgcc cggattctct cagaaccggg gccggggttg acggaattga ccggggcggc
      781 gaagcaagtg gccgcggtca gccgggaagg ccggcagggt ccagtttcgc cagcagccgg
      841 gtcagggttt cgagttcctc gttgtcgagg gcttccatcg agagtggcgg ctggcgaaga
      901 aaggcgttga cccggccctg gaccttgcgg cccttggcgg tgaggaccag caggcggacc
      961 cggcggtcgt cgggatcgag ggtgcgcttg accaggccgc gttcctccag acggtcggtg
     1021 atcccggtca ggttcgagct gt
//
LOCUS       DAOQFI010000812         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_123516_length_1036_cov_3.590214, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000812 DAOQFI010000000
VERSION     DAOQFI010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123516_length_1036_cov_3.590214"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /gene="ribD"
                     /locus_tag="PLE93_11680"
     CDS             complement(<1..780)
                     /gene="ribD"
                     /locus_tag="PLE93_11680"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNH87754.1"
                     /translation="MPAAQPTSEADYWQRAIQLAEHGLGWTSPNPAVGAVLVKDGRVI
                     GEGWHRRHGELHAEREALADAERRGNDPAGATIFVTLEPCAHTGSQPPCADGLIEAGV
                     GEVVIAAGDPTEKTRGVGPARLEDAGIRVRWAEGPAADAALALIQDFRKRALTGKPLV
                     TLKMAMSLDGKVATRTGDSKWISGEESRALVHRWRAEMDAVAVGSGTLTADDPRLTAR
                     HTDGEPVRQPARVIFDSGLLITTGAALFADIDEAPLLIIAGP"
     gene            799..>1036
                     /locus_tag="PLE93_11685"
     CDS             799..>1036
                     /locus_tag="PLE93_11685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH87755.1"
                     /translation="MKVIIQIPCLNEEETLPATLADLPREIEGVDEIETLVIDDGSTD
                     RTIEVARQCGVDHIVKLPNNRGLATGFQSGLDACL"
BASE COUNT          162 a          357 c          343 g          174 t
ORIGIN      
        1 gggaccggca atgatcaaga gcggcgcctc gtcgatatcg gcgaagaggg ctgccccggt
       61 cgtgatgagc agcccagagt caaagatcac cctcgccggc tgccggaccg gttcgccgtc
      121 ggtgtggcgc gcggtcagtc tcgggtcgtc ggcggtcaag gtgccggagc cgaccgccac
      181 cgcgtccatc tcggcccgcc agcggtgaac gagtgcccgg ctctcttcgc cggaaatcca
      241 ttttgagtcc ccggtccggg tcgcgacctt gccgtcgagg ctcatcgcca tcttcaaggt
      301 aaccaggggt ttgccggtca gggcccgctt gcgaaagtcc tgaatcaggg cgagcgccgc
      361 gtcggcagcc ggcccttcgg cccagcggac ccggatcccg gcgtcttcga gccgggccgg
      421 gccgacgccc cgggtctttt cggtcgggtc cccggcggcg atcaccacct cgcctacccc
      481 ggcctcaatc agcccgtcgg cgcagggcgg ctggctgccg gtgtgagcgc agggttcgag
      541 ggtcacgaag atcgtggcgc cggccgggtc gttgccgcgc ctttccgcat cagccaatgc
      601 ctcccgttcg gcgtggagct ccccgtggcg gcgatgccag ccttcaccga tcacccggcc
      661 gtccttgacc agcaccgcac cgaccgccgg attggggctg gtccagccga ggccgtgttc
      721 ggccagttgg atggcccttt gccagtagtc ggcctcggag gtgggttgag cggcaggcat
      781 gggagaatcc tcagcctaat gaaggtaatc atccagatcc cctgcctcaa cgaggaggag
      841 accctgcctg caactctcgc ggacctgccg cgcgagatcg aaggcgtgga tgaaatcgag
      901 accctggtca tcgatgacgg ctcaaccgac cggacgatcg aagtcgcccg gcaatgcggc
      961 gtcgaccaca tcgtcaagct gcccaacaac cgcggtctgg caaccggttt ccagtccgga
     1021 cttgatgcct gtctca
//
LOCUS       DAOQFI010000813         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_123806_length_1035_cov_2.584694, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000813 DAOQFI010000000
VERSION     DAOQFI010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123806_length_1035_cov_2.584694"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      198..358
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            406..>1035
                     /locus_tag="PLE93_11690"
     CDS             406..>1035
                     /locus_tag="PLE93_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87756.1"
                     /translation="MACLTPASFGSTAASGATSGGGGLVIPELPEISDVICLTGCTKL
                     REVSAGGSVQITGTNMKSVSYVAFRGTNRNIRVKPDVVTETRVEATVPDGAASGRVRV
                     VSNTNSVSDPSSQILTVGEGQFTRTGKLTITDASTSPTKAYQYGLRRPVLNFVVNGST
                     PRVDLRVDVVNSSGDVVRSRFLNDVPTGSTEKVGWSGLLARRKTAPNGSY"
BASE COUNT          200 a          327 c          320 g          188 t
ORIGIN      
        1 cgacgcgcgg ccacggaata ataggggaat cacagttgaa gggtcttctc gtcggctggc
       61 cgcaagacag agtcggccca tccattctta tgttctgtgg gccgacgggt tgctttggga
      121 ggtccggcga agaccggatc gacttcttac gttgacaaac atcacacccg acgtcgtggg
      181 gcctgtctat actgacgcgc gtcaataagc cgaacccttt ccttccaggg gaaggggcgg
      241 aggaaccaac ggggaactgt ttccccaggg gcgaatcgga ggcaagcatt gctccgtagc
      301 gcatctcttt cagcgcgagc ccgccagcta actccgcagg caaccggaaa gagattgaag
      361 acgagtagac gaaaaacctt ctcgctcgcc gcttttgcgg tcgcgttggc ctgcctgacc
      421 ccggcctcct tcggctcgac cgcggccagc ggcgcaacca gcggtggcgg cggactggtg
      481 attcccgaac tgcccgagat ctccgatgtg atctgcctga ccggctgcac caagctccgc
      541 gaggtttcgg ccggaggctc ggtccagatc accggtacga acatgaagtc ggtctcctat
      601 gtggcattcc gcggtacgaa taggaacatc cgggtgaagc cggatgtggt gaccgagacc
      661 agggttgaag ccacggttcc cgacggcgcg gcctcgggcc gggttcgcgt cgtctccaac
      721 accaactcgg tttccgatcc gtccagccag atcctcacgg tcggcgaggg gcagttcacc
      781 cgcaccggca agctgacgat caccgacgcc agcacttccc cgaccaaggc ctaccagtac
      841 gggctgcgcc gtccggtcct caacttcgtg gtcaacggct cgaccccgag ggtggacctc
      901 cgggtcgatg tagtcaattc atcgggtgat gtggtccgca gccggttcct caatgacgtg
      961 ccgacgggat caaccgaaaa ggtcggctgg agcggacttc tcgccaggcg taagaccgcc
     1021 ccgaatggct cctac
//
LOCUS       DAOQFI010000814         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_126903_length_1022_cov_4.657704, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000814 DAOQFI010000000
VERSION     DAOQFI010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126903_length_1022_cov_4.657704"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLE93_11695"
     CDS             complement(<1..676)
                     /locus_tag="PLE93_11695"
                     /inference="COORDINATES: protein motif:HMM:NF015441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNH87757.1"
                     /translation="MAGTVSWISIAPVKGMRIQSLNEVELTCDGVPGDRAFFLADEDG
                     AMVNGKRFGSLMEIIPAHDRESGRLSLEFPDGATVEGEVGLGEPEEVTFFGQPERARP
                     VTGDFSDAVSRHTGARLRLMAHPEGRPGVDRGKWGGVTLLGAASLESLREAGRDFNCA
                     EPSSEALPGGTASDPDPGPIDGRRFRMTFGVEGTRPYEEDDWTGREIRIGEALVTLDT
                     QALQVVR"
     gene            complement(700..>1022)
                     /locus_tag="PLE93_11700"
     CDS             complement(700..>1022)
                     /locus_tag="PLE93_11700"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNH87758.1"
                     /translation="VTASDAPRDPLLVIVTGQAKALLGAREARPQYFLGGLIGLIVVV
                     TSLSAVTLIGAAGLTAALVTGQMIGSLLLDRFGAFGLTRIPIDRVRLAAAAALLIGTF
                     LATG"
BASE COUNT          166 a          366 c          346 g          144 t
ORIGIN      
        1 ggcggacgac ctgcagggcc tgcgtgtcga gcgtgaccag ggcttcgccg atccgaattt
       61 cccggccggt ccagtcgtcc tcctcataag gcctggttcc ttcgacgcca aaggtcatgc
      121 ggaagcgccg cccgtcgatc ggccccgggt cgggatcgct cgcagttccg cccggcagcg
      181 cttcgcttga aggctccgcg cagttgaaat cccgccccgc ctcgcgcagg ctctccagcg
      241 aagccgcacc gagcagggtc acgccgcccc atttgccgcg atccaccccg ggccgccctt
      301 ccgggtgggc catcagccgc agcctggccc cagtgtgcct cgagaccgcg tcggagaaat
      361 caccggtgac cggccgggcc cgctcgggct ggccgaagaa agtgacctcc tcgggctcac
      421 cgagcccgac ctcgccctca acggtcgcgc cgtcgggaaa ctcaaggctc agcctgcccg
      481 attcccggtc gtgtgcgggg atgatttcca tgagcgaacc gaagcgcttg ccgttgacca
      541 tcgcgccgtc ttcatcggcg aggaagaagg cccggtcgcc ggggactccg tcgcaggtca
      601 actcgacctc gttcagcgac tggatgcgca tccccttgac cggggcgatc gatatccagg
      661 agacggttcc ggccacgccc ggatcctctc atgcccggat cagccggtgg cgaggaaggt
      721 gccgatcagc agggccgccg ccgcggccag ccggacgcgg tcgatcggga tgcgggtcag
      781 gccgaaggcg ccaaaccggt cgagcagcag cgagccgatc atctggccgg tgaccagggc
      841 cgcggtcagg ccggccgcac cgatcaaggt cacggctgaa agcgaagtga cgaccacgat
      901 caggccgatc aggccgccaa ggaagtactg cggccgggcc tcgcgggccc cgagcagcgc
      961 ctttgcctgc ccggtgacga tcaccagcag ggggtcgcgg ggggcgtcag aagcggtcac
     1021 cg
//
LOCUS       DAOQFI010000815         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_127499_length_1020_cov_2.107772, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000815 DAOQFI010000000
VERSION     DAOQFI010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127499_length_1020_cov_2.107772"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..703)
                     /gene="rfbC"
                     /locus_tag="PLE93_11705"
     CDS             complement(155..703)
                     /gene="rfbC"
                     /locus_tag="PLE93_11705"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNH87759.1"
                     /translation="MARRLETGLEGLALIEPQVFGDERGFFVETFSRDAWRELGVEAD
                     FVQHNHSRSSKGTLRGLHFQTSPGQAKLLRCPRGKIFDVAVDLRRDSATYGKWEGYVL
                     DDEKHHQLYVPVGFAHGFCVLSELADVTYLVSSLYDPETEAGIRWNDPEIGVEWPVGE
                     PLLSQRDIEAPTLGEVAAGLPW"
     gene            complement(706..>1020)
                     /locus_tag="PLE93_11710"
     CDS             complement(706..>1020)
                     /locus_tag="PLE93_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNH87760.1"
                     /translation="AGQVYNVGGPDELPNIEVVRTILELTGRDESLITHVGDRLGHDV
                     RYSLASEKTEGLGWEAAVAFREGIAKTVDWYRENEWWWGPLRSGEYRHYYQRQYGQAL
                     SE"
BASE COUNT          162 a          349 c          338 g          171 t
ORIGIN      
        1 gcctgatccg caagcggggc tttaccgcca ggggttcggt ctacgccaag gtgcggggcg
       61 gccagaccgc ggttgccttc ggcctctgga agaccaagga catctaccag ccgcccttcg
      121 gctgatctga ctcaccccgg tcaccggctt cgccctacca gggcaggccg gcggccactt
      181 caccgagcgt aggcgcctcg atgtcccgct gtgaaagcag cggttccccg accggccact
      241 cgaccccgat ttcggggtcg ttccagcgga tcccggcttc ggtctccggg tcgtagaggc
      301 tggaaaccag gtaggtgacg tcggccagtt cggacagcac gcagaagccg tgggcgaagc
      361 cgaccggcac gtagagctga tggtgcttct cgtcatcgag gacgtatccc tcccacttgc
      421 cgtaggtggc cgagtcgcgg cgcaggtcga cggcgacgtc gaagatcttc ccgcgggggc
      481 agcgcaggag cttcgcctgg ccgggagaag tctggaagtg aaggccgcgc agcgtgccct
      541 tcgaggagcg ggagtggttg tgctggacga agtccgcctc caccccgagc tcgcgccagg
      601 cgtcccggct gaaggtctcc acgaagaagc cgcgctcatc cccgaagacc tgcggttcga
      661 tcagcgccag accttccagt ccggtttcca gtcgccgggc catgcctact cggagagcgc
      721 ctggccgtac tgccgctggt agtagtggcg gtactcgccg gaacggagcg ggccccacca
      781 ccactcgttc tcgcgatacc agtcaaccgt cttcgcgatg ccctcgcgga aggcgaccgc
      841 cgcttcccag ccgagtccct cggtcttctc cgaggcaagc gagtaacgga catcgtggcc
      901 gagccggtcg ccgacgtggg tgatcagcga ttcgtcccgc ccggtcagtt cgaggatcgt
      961 ccggaccacc tcgatgttcg ggagctcgtc ggggccaccg acgttgtaga cctgcccggc
//
LOCUS       DAOQFI010000816         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_128477_length_1016_cov_1.935484, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000816 DAOQFI010000000
VERSION     DAOQFI010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128477_length_1016_cov_1.935484"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLE93_11715"
     CDS             <1..635
                     /locus_tag="PLE93_11715"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HNH87761.1"
                     /translation="FNFCVGTGEATFIGASPELLVRCAGRGVSTVGLAGSTRRSSDPA
                     VDDHLAQQLLGSSKDRGEQAVVVRRIVKSLSKLSVWVEAADEPEIIKVANIQHLGTPV
                     YAQLAQPRTAIELAGLLHPTPAVGGEPWKKARRVMAEVERMDRGWYAGPVGWMDGSGD
                     GEFCVALRSALIRDREAHLFAGVGVVADSDPAAELAETEIKLGALLPLLS"
     gene            complement(701..>1016)
                     /locus_tag="PLE93_11720"
     CDS             complement(701..>1016)
                     /locus_tag="PLE93_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH87762.1"
                     /translation="VITGDLGFFHDVGSLALARGLEVPVRIVVLNNDGGGIFERLPQK
                     QAMTGGEFEQLMRTPSGLSVEDAARLYGLPFHLAPRLDALEAAFTADAESVSVIEVPV
                     TG"
BASE COUNT          166 a          354 c          349 g          147 t
ORIGIN      
        1 gtttcaactt ctgcgtcggg accggcgagg ccaccttcat cggggccagc ccggagcttc
       61 tggttcgctg cgccggccgc ggcgtttcca cggtcggcct ggccggatcg acccgccgaa
      121 gcagcgaccc ggcggttgac gaccatctcg cccagcagct tctgggcagc agcaaggacc
      181 ggggcgaaca ggcggtggtg gtgcgacgga tcgtcaagtc gctcagcaaa ctgagcgtct
      241 gggtcgaggc ggcggacgag ccggagatca tcaaggtggc caacatccag cacctcggga
      301 caccggtcta cgcccagctc gcccagcccc gcaccgcaat cgagctcgcc ggtctgctcc
      361 atccgacccc tgcggtcggc ggtgagccct ggaaaaaggc ccgcagggtc atggccgagg
      421 tcgaacggat ggaccggggc tggtacgccg gcccggtcgg gtggatggac ggctccggcg
      481 acggcgagtt ctgtgtcgcc ctgcggagcg ccctgattcg cgaccgcgag gcccatctct
      541 tcgccggggt cggggtggtc gccgattccg acccggccgc cgaactggcc gagaccgaga
      601 tcaagctcgg cgccctgctg cccctgctca gctagccacc ctctcgtcga gaacccgctt
      661 tgcgccacat accggcacaa accgggcact cgccgcggat tcagccggtg accgggactt
      721 cgatcaccga gaccgattcg gcgtcagccg tgaaggcggc ctcgagcgcg tcaagccggg
      781 gagcgaggtg gaagggcagg ccgtagagcc gcgccgcatc ctcgacgctc aggccggaag
      841 gcgtccgcat caactgctcg aactcgcccc cggtcattgc ctgcttctgc ggcaggcgct
      901 cgaagattcc gccgccatcg ttgttcagga ccacgatccg gaccgggacc tcgaggccgc
      961 gggccagggc gaggctgccg acgtcatgga agaagccgag atcaccggtg atgacc
//
LOCUS       DAOQFI010000817         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_128611_length_1015_cov_2.978125, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000817 DAOQFI010000000
VERSION     DAOQFI010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128611_length_1015_cov_2.978125"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..933)
                     /locus_tag="PLE93_11725"
     CDS             complement(256..933)
                     /locus_tag="PLE93_11725"
                     /inference="COORDINATES: protein motif:HMM:NF022805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HNH87763.1"
                     /translation="MSEVHRTEDQVEVELDQKEEINLLDELGGPQGIADSSIPGLVFV
                     IAYTVSGQKLELAAGVAVGIALLIALIRLVRGERARYAASGFFGVALAAFIATRTGKA
                     EDFFLPGLLFNAGYSLAYAISIAIRWPLMGVFIGPLIGEGMTWREDPERVKLFNKISW
                     LWVGTFLLRLAVQLPLYLSGALVALGIAKTAMGLPIFALAIYLSYLMLKAAGIDLQQK
                     KEAEEAG"
BASE COUNT          191 a          327 c          316 g          181 t
ORIGIN      
        1 gtcccggtac tcgcccggcc tctccggtgg gtgggtccgg accaccattt cgaaggggcg
       61 aatccggtac aggccttcgg aagcgccgac ccgaagtcgg atcggaacgt ccttgcgctt
      121 cgggcgcggg ttcgcgtcgc cattggcgcc ggcgcgacct ggcgcctcgc cggttctggt
      181 gggtgatgaa gacggagaca aaagcggcca gacattgtat gcctttgccg cggcggggcc
      241 gttcacgccc ggcgatcagc ccgcttcttc ggcttctttc ttctgctgaa ggtcgatgcc
      301 ggcggctttc agcatcaggt agctgagata tatcgcaagc gcaaagatcg gcagtcccat
      361 cgcggttttc gcgattccca gagcgaccag tgctcccgaa agatagaggg gcaactgcac
      421 ggccaggcga agcagaaagg ttccgaccca aagccagctg atcttgttga acagcttgac
      481 ccgctcggga tcttcacgcc aggtcattcc ctccccgatc aggggcccga tgaagacgcc
      541 catcaggggc cagcggatcg cgattgaaat cgcgtatgcg agcgagtaac cggcgttgaa
      601 cagcagcccc ggaagaaaga aatcctcggc cttgccggtt cgggtcgcaa tgaaggcagc
      661 cagggcgaca ccgaagaagc cggaagcggc atagcgggcc cgctcccccc ggaccagtct
      721 gatcagcgcg atcagcaacg cgattccgac cgcgaccccg gccgccagtt ccagcttctg
      781 cccactgacc gtgtaggcga tgacgaagac cagcccggga atgctcgaat cggctattcc
      841 ctggggtccg cccagctcgt caaggaggtt gatctcctct ttctggtcga gctcgacttc
      901 gacctgatcc tctgtcctgt gaacttcgct caccggccga ttgtctcagc ccgaaccggc
      961 cgatggcggc aaggcgcgtg aacgtcaggc cgcgaggcgg tcttcgaaca ctgcc
//
LOCUS       DAOQFI010000818         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_128625_length_1015_cov_2.843750, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000818 DAOQFI010000000
VERSION     DAOQFI010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128625_length_1015_cov_2.843750"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>1015)
                     /locus_tag="PLE93_11730"
     CDS             complement(115..>1015)
                     /locus_tag="PLE93_11730"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNH87764.1"
                     /translation="GLAVDLGRSWILIDCGEGTQRQMRRSMGLVQVDEIYLTHFHLDH
                     CLGLPGLLKTYDLNDREEPLMLFGPVGLHGLMKDLQPLIGRLDYHLEVEELEGGDVVP
                     HVDYEIECFPVEHRTRALGYALLEDDRPGRFDPAEAERLGVKAGPDFKRLQDGEEVDG
                     RDGKVRPDQVMGEDRLGRAVALTGDTGPCKATVEAARDADLLIHDASFMVEDAGRAKE
                     TGHSTGTEAAEVAAEAGAKMLALVHISSRYHVGKALEEARAVFTRTVAPRDFDTVEIP
                     FPERGEPILVPNGARRETQPATE"
BASE COUNT          150 a          335 c          337 g          193 t
ORIGIN      
        1 gccggagtcg atgttggccg aaggtgccgc aattctggcg ccattacggt gctggatgtg
       61 cggcaccttt gaggttcgat cgggctcttt cagccgtggc caattccggg aggcctactc
      121 ggtggctggc tgggtttctc gcctggctcc gttagggacg aggatcggtt cgcctcgttc
      181 ggggaagggg atttcgaccg tgtcgaagtc gcggggagcg accgtcctgg tgaagaccgc
      241 cctggcctcc tcgagggcct taccgacgtg atagcgggaa gagatgtgaa cgagggcgag
      301 catcttcgcg ccggcctcgg ccgccacttc ggccgcttcg gtcccggtcg aatgcccggt
      361 ttccttcgcc cgcccggcgt cctcgaccat gaagctggcg tcgtggatca acaggtcggc
      421 gtcacgagca gcttccacgg ttgccttgca gggtccggtg tctccggtca gtgccactgc
      481 ccggccgagc cgatcctcgc ccatcacctg gtccggccgg accttgccgt cccttccatc
      541 gacctcctcc ccgtcctgaa gcctcttgaa gtcgggcccg gccttcacgc cgagccgctc
      601 cgcctctgcg gggtcgaagc ggccgggccg gtcatcctcg aggagggcgt agccgagcgc
      661 ccgggtgcgg tgttcgaccg ggaaacactc gatctcgtaa tcgacgtggg gaaccacgtc
      721 tccgccttcg agctcctcga cctcgaggtg gtaatcgagc cggccgatca gcggctgcag
      781 gtccttcatc agcccgtgaa ggccgaccgg gccgaagagc atgagcggct cctcgcggtc
      841 attcaggtca taggtcttga gcagaccggg caggccgagg cagtgatcga ggtggaagtg
      901 agtcaggtag atctcgtcga cctggaccag ccccatcgat cgccgcatct gccgctgggt
      961 tccctcgccg cagtcgatca gaatccatga gcgtccgagg tcgaccgcca gccca
//
LOCUS       DAOQFI010000819         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_129450_length_1012_cov_2.477534, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000819 DAOQFI010000000
VERSION     DAOQFI010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129450_length_1012_cov_2.477534"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLE93_11735"
     CDS             <1..803
                     /locus_tag="PLE93_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87765.1"
                     /translation="EGRQHRMPADTPTIVATSMVVSGLHRVWQVFEDEQARSLTVDSS
                     WFVLDDLPRTSDTGRSFCWAYSPVDEQQVLNATLKGSRLIAQAIDAGLAGGETDRARK
                     AATASARFVIEYQEGDGGWPYAVGSDSRTWRDHHHTGYVLECLSTYRSILSDRQFDSE
                     IEKGWDHYRSSFFNRDNLPRYYDDRDGPLDATAAGQAFITLAQFDDVEFGFEVAGACL
                     PVLARPDGTFTYRRTGRRLTRTHFIRWSTAWMFAGMARLLSKESRFPR"
     gene            800..>1012
                     /locus_tag="PLE93_11740"
     CDS             800..>1012
                     /locus_tag="PLE93_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH87766.1"
                     /translation="MTYWVDVDNPPQAQYLSPIARELRDRGREVLVTARNHAPTLEVL
                     ANRGEVAVPVGGTFGTSSVSKVLGTLR"
BASE COUNT          182 a          322 c          317 g          191 t
ORIGIN      
        1 gggaggggag gcagcaccgc atgccagccg atacaccgac gatcgtcgcg acctcgatgg
       61 tggtcagcgg ccttcaccgg gtctggcagg tcttcgagga tgaacaggcc aggagtctga
      121 ccgttgacag ctcctggttc gtcctcgacg atctgccccg aacatcagac accgggcgat
      181 cgttctgctg ggcctattct ccggtcgacg agcagcaggt tctgaacgca acgctgaagg
      241 gcagccggtt gatcgcccag gcgatcgatg cgggactggc cggcggcgag actgaccgtg
      301 cccggaaggc agcaacggcc tcagcccggt tcgtcatcga atatcaggag ggagacggcg
      361 gctggcccta cgccgtcggc tcggattcga gaacgtggcg cgaccatcac cacaccggct
      421 acgtgctgga gtgcttaagc acctatcgct cgattctgtc tgaccgccag ttcgattctg
      481 agatagagaa gggctgggat cactacaggt ccagcttctt caaccgggat aatcttccgc
      541 gttactacga cgaccgtgac ggtccgctcg acgcgaccgc cgccggccag gcgttcatca
      601 cacttgccca gttcgacgac gtcgaattcg gattcgaggt cgccggtgcc tgcctgccgg
      661 ttctggcccg gcccgacggt accttcacgt acaggcggac cggcaggcgc ctgacccgga
      721 cgcatttcat ccgttggtca acggcctgga tgttcgccgg aatggcgcgt cttctctcca
      781 aagagtcccg ttttccccga tgacttactg ggttgatgtc gacaatccgc cccaggctca
      841 gtacctgagt ccgatcgccc gggaactccg ggatcgtgga cgtgaggtcc tggtcaccgc
      901 ccgaaaccac gctccgaccc tggaggtact cgcgaaccgt ggcgaagtgg cagttccggt
      961 gggggggact ttcggaacct cctcggtttc gaaagtactc ggaaccctcc gg
//
LOCUS       DAOQFI010000820         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_131711_length_1003_cov_4.363924, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000820 DAOQFI010000000
VERSION     DAOQFI010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131711_length_1003_cov_4.363924"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLE93_11745"
     CDS             <1..983
                     /locus_tag="PLE93_11745"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNH87767.1"
                     /translation="ADTKVTAPEATYAPADEAMCDRARERFTADRNRRLPRRKSKTAT
                     LPNMIIIGGLKCGTTSIHHYLGVHPDIHMSKPKELNFFAAEQNWDLGFDWYENRFDST
                     VRINGESSPHYTNRPRFNDVAERIHKHIPDVKLIYMVRDPIKRILSHWVHATGAGYET
                     REFVETLSLPDTAYIQRSQYWMQLQPYLEYFDRDQIKVISQEDLGKKRDETMKDAFAY
                     LGVDESFTSEQWDRQWEKSSAKHDDKYQFMEKLIKLPGFRAFDRNFDRLPESMRWMVE
                     KVVHDPDKPPAPKPEIPDDLMETLKDRFRDDVAGLKQWSGRDFDGWHDYS"
BASE COUNT          224 a          344 c          276 g          159 t
ORIGIN      
        1 ccgccgatac aaaagtgacc gcccccgagg ccacctacgc ccccgctgac gaagcgatgt
       61 gcgaccgcgc ccgcgagcgc ttcaccgccg atcgcaaccg tcgcctgccg cgccgcaaga
      121 gcaagaccgc gaccctgccg aacatgatca tcatcggcgg cctgaagtgc ggaaccactt
      181 cgatccacca ctacctcggc gtccaccccg acattcacat gtcgaagccg aaggaactca
      241 acttcttcgc cgccgaacag aactgggatc tcggtttcga ctggtacgaa aaccgcttcg
      301 attcgaccgt caggatcaac ggcgaatcct ccccgcatta cacaaaccgc ccccgcttca
      361 acgacgtcgc cgagcggatc cacaagcaca tccccgacgt gaagctgatc tacatggtcc
      421 gcgacccgat caagcgaatc ctttcccact gggtccacgc gaccggagcc ggttatgaaa
      481 cccgggaatt cgtcgagacc ctctcgctcc cggacaccgc ctacatccag cgttcgcagt
      541 actggatgca gctgcagcct tacctcgagt acttcgaccg ggaccagatc aaggtcatct
      601 cccaggaaga ccttggcaag aagcgggacg agacgatgaa ggatgcgttc gcctacctcg
      661 gagttgacga gagcttcacc tccgagcagt gggaccgcca atgggagaag tcttcggcca
      721 agcacgacga caagtaccag ttcatggaaa agctgatcaa gctgcccggg ttccgtgcct
      781 ttgaccggaa tttcgaccgt ctgccggagt cgatgcgctg gatggtcgag aaggtcgttc
      841 acgacccgga caagccgccg gccccgaagc cggagatccc ggatgacctg atggaaaccc
      901 tcaaggaccg gttccgcgat gacgtcgccg gcctgaagca gtggtcgggc cgcgacttcg
      961 acggctggca cgactactcc tgagcagtcg cgcccggaaa gcg
//
LOCUS       DAOQFI010000821         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0708
            NODE_132024_length_1002_cov_2.826822, whole genome shotgun
            sequence.
ACCESSION   DAOQFI010000821 DAOQFI010000000
VERSION     DAOQFI010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810340
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:37:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,320
            Genes (coding)                    :: 2,314
            CDSs (with protein)               :: 2,314
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132024_length_1002_cov_2.826822"
                     /isolate="AMR_MDS_0708"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLE93_11750"
     CDS             complement(<1..364)
                     /locus_tag="PLE93_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1727 domain-containing protein"
                     /protein_id="HNH87768.1"
                     /translation="MIKAKASVARMVGRLSRATGKGGGTTLPGRVLLKLDANAIDKLG
                     ARLTDGTTLISATNGKTTTASMLATVLAGAGRHPVHNRAGSNMTWGVATALTEQRGCE
                     GLFEVDEAWLPEVADRLGP"
     gene            complement(361..>1002)
                     /locus_tag="PLE93_11755"
     CDS             complement(361..>1002)
                     /locus_tag="PLE93_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNH87769.1"
                     /translation="KVGLIPAGRGNDLARGLGIPTDPVQAVDCLAAGHTRAIDVGDAN
                     GKRFLGIASVGFDSDANRIANEARLLKGQLVYVYAAVRALLAWKPARFSLVENGVQSR
                     LTGYNVVVANNGFYGGGMNVAPDARIDDGQLDVITVGEAGKIRFLLNFPKVFKGTHVE
                     EVESVSFHRTSVIEIGASRPFTIYADGDPLTELPATIRVIPSALQVIVPEPAS"
BASE COUNT          148 a          329 c          356 g          169 t
ORIGIN      
        1 ggggtccgag acggtcggcg acctccggta gccaggcctc gtcaacctcg aagagtcctt
       61 cgcagccgcg ctgctcggtc agggcggtgg ccacacccca ggtcatgttc gagccggccc
      121 ggttgtggac cggatgccgg ccggcgccgg caaggaccgt ggccagcatc gaggcggtgg
      181 tcgtcttgcc gttggtcgcg ctgatcaggg tcgtgccgtc ggtcaggcgg gcgcccagct
      241 tgtcgatcgc gttggcgtcc agcttcagca ggacccggcc gggcagggtg gtgccgccgc
      301 ccttgcccgt ggcgcggctc agccggccga ccattcgggc gacgctggct tttgccttga
      361 tcaagaggcc ggctccggca cgatcacctg aagcgccgag gggatgaccc ggatcgtcgc
      421 cggaagttcg gtcagcggat caccgtccgc gtagatcgtg aacgggcggc tggcaccgat
      481 ctcgatcacg ctggtgcggt ggaacgagac cgactcgacc tcttcgacat gggttccctt
      541 gaacaccttc gggaaattga gcaggaagcg gatcttgccg gcttcgccga ccgtgatcac
      601 atcgagctgg ccgtcgtcga tccgggcgtc aggggccacg ttcatgccgc cgccgtaaaa
      661 gccgttgttg gcgacgacca cgttgtagcc ggtcaggcgg ctctggacgc cgttctcgac
      721 caggctgaag cgggccggct tccaggccag cagcgccctt accgccgcat agacatagac
      781 gagctggccc ttcaggagcc ttgcctcgtt ggcgatccgg ttcgcatcgg agtcgaaccc
      841 gaccgaggcg atgccgagga agcgcttgcc gttggcatcg ccgacgtcga tcgcgcgggt
      901 gtggccggcg gccaggcagt cgacggcctg aaccggatct gtggggatgc cgagcccgcg
      961 ggccaggtcg ttgccgcgac cggccggaat cagcccgacc tt
//
